谷类第4组主要致敏原的dna序列和重组制品的制作方法

专利名称：谷类第4组主要致敏原的dna序列和重组制品的制作方法
背景技术：
本发明涉及到来自谷类(小麦族(Triticeae))的第4组主要致敏原的DNA序列的提供，本发明也包括具有降低过敏作用的片段、部分序列的新组合及含有点突变的突变体。重组的DNA分子和其衍生而来的多肽、片段、部分序列的新组合和变异体可以被用于花粉过敏性疾病的治疗。由重组方法制备的蛋白可以用于花粉过敏的体外和体内诊断。
I型过敏反应是全球性的，在工业国家中有多至20％的人口患有如过敏性鼻炎、结膜炎或支气管哮喘。这些过敏由从各种来源如植物花粉、螨类、猫和狗释放到空气中的致敏原(空气致敏原)引起，多至40％的I型过敏患者有对禾本花粉致敏原的特异性IgE反应，对谷类花粉致敏原尤甚(Freidhoff et al.，1986，J.Allergy Clin.Immunol.78，1190-2001)，在谷类花粉致敏原中，又以黑麦致敏原为最重要。
触发I型过敏反应的物质为蛋白、糖蛋白或多肽，通过粘膜被摄取后，在已被致敏的个体体内这些致敏原与结合于肥大细胞表面的I，分子反应，如果两个IgE分子通过致敏原相互交叉连接，即会导致效应细胞释放介质(如组胺、前列腺素)和细胞因子，并引起相应的临床症状。
根据各种致敏原与过敏患者的IgE反应的相对频率，将致敏原区分为主要致敏原和次要致敏原。
禾本科(Poaceae)来源的各种花粉被划分为不同组，各组之间相互同源。
特别值得一提的是主要致敏原第4组中的分子相互间具有很强的交叉免疫反应，对鼠单克隆抗体和人IgE抗体均如此(Fahlbusch et1993 Clin.Exp.Allergy 2351-60；Leduc-Brodard et 1996，J.AllergyClin.Immunol.981065-1072；Su et 1996，J.Allergy Clin.Immunol.97210；et 1998，Clin.Exp.Allergy 28799-807；Gavrovic-et 2000，10(6)361-367；Stumvoll et al.2002，Biol.Chem.3831383-1396；Grote et2002，Chem.3831441-1445；Andersson und Lidholm，2003，Arch.Allergy Immunol.13087-107；Mari，Clin.Exp.Allergy，33(1)43-51)。
迄今为止，尚没有一个第4组主要致敏原的完整DNA序列被弄清楚。
迄今第4组致敏原中的鸭茅(Dactylus glomerata)致敏原只可能通过酶消化获得多肽并进行序列测定DIYNYMEPYVSK(SEQ ID NO 13)，VDPTDYFGNEQ(SEQ ID NO 14)，ARTAWVDSGAQLGELSY(SEQ ID NO 15)和GVLFNIQYVNYWFAP(SEQ NO 16，Leduc-Brodard et J.AllergyClin.Immunol.981065-1072)。
通过蛋白水解获得肽并测定了序列的还有致敏原第4组中的亚热带狗牙根(Cynodon dactylon)KTVKPLYIITP(SEQ ID NO 17)KQVERDFLTSLTKDIPQLYLKS(SEQ ID NO 18)TVKPLYIITPITAAMI(SEQ ID NO 19)LRKYGTAADNVIDAKVVDAQGRLL(SEQ ID NO 20)KWQTVAPALPDPNM(SEQ ID NO 21)VTWIESVPYIPMGDK(SEQ ID NO 22)GTVRDLLXRTSNIKAFGKY(SEQ ID NO 23)TSNIKAFGKYKSDYVLEPIPKKS(SEQ ID NO 24)YRDLDLGVNQVVG(SEQ ID NO 25)SATPPTHRSGVLFNI(SEQ ID NO 26)和AAAALPTQVTRDIYAFMTPYVSKNPRQAYVNYRDLD(SEQ IDNO 17，Liaw et 2001，Biochem.Biophys.Research Communication 280738-743)).
对于黑麦草(Lolium perenne)，作为第4组基本致敏原的具有以下序列的肽片段已有描述FLEPVLGLIFPAGV(SEQ ID NO 28)和GLIEFPAGV(SEQ ID NO 29 Jaggi et 1989，Int.Arch.342-348)。
尚未发表的来自梯牧草(Phleum pratense)的PhI p 4序列(SEQ IDNO 11)，作为第一个第4组致敏原序列，本专利申请的发明者已在国际申请WO 04/000881中对其进行了阐述。
迄今为止还不知道来自谷类(小麦族(Triceae))的第4组致敏原的任何序列。
因此，本发明的目的是要提供来自谷类的第4组致敏原的DNA序列，特别是来自黑麦(Secale cerale)的Sec c 4(SEQ ID NO 1、3)、来自大麦(Hordeum vulgare)的Hor v 4(SEQ ID NO 5)和来自小麦(Triticum aestivum)的Tri a 4(SEQ ID NO 7、9)以及相应的重组DNA分子，以这些重组DNA分子为基础，致敏原能以无修饰的或修饰过的蛋白形式表达进而得到，用于有意义的药物开发。PhI p 4序列(SEQID NO 11)是本发明的起始点。
本发明的序列表与SEQ ID NO 1-10对应的成熟致敏原的DNA和蛋白序列前面带有信号序列，其编码区终止于DNA序列上的TGA或TAG终止密码子。
-Sec c 4DNA序列(a)同工型Sec c 4.01(SEQ ID NO 1)，(b)同工型Secc 4.02(SEQ ID NO 3)-来源于与SEQ ID NO 1和3相应的DNA序列的蛋白序列(SEQ IDNO 2、4)-Hor v 4的DNA序列(SEQ ID NO 5)-来源于与SEQ ID NO 5相应的DNA序列的蛋白序列(SEQ ID NO 6)
-Tria 4 DNA序列(a)同工型Tria 4.01(SEQ ID NO 7)，(b)同工型Tria 4.02(SEQ ID NO 9)-来源于与SEQ ID NO 7和9相应的DNA序列的蛋白序列(SEQ IDNO 8、10)-对应于WO 04/000881中的SEQ ID NO 5的PhI p 4DNA序列(SEQID NO 11)-对应于WO 04/000881中的SEQ ID NO 6的PhI p 4蛋白序列(SEQID NO 12)发明描述本发明目前第一次提供谷类花粉主要致敏原Sec c 4、Hor v 4和Tria 4的DNA序列，对应的序列号为SEQ ID NO1、3、5、7和9。本发明因此涉及到选自对应于SEQ ID NO1、3、5、7和9的核酸序列的DNA分子。
本发明进一步涉及到与本发明的DNA序列同源的序列和来源于其他禾本科(poaceae)的第4组致敏原的相应的DNA分子，这类植物有黑麦草(Lolium perenne)、鸭茅(Dactylis glomerata)、草地早熟禾(Poapratensis)、狗牙根(Cynodon dactylon)和绒毛草(Holcus Lanatus)，由于存在同源性，它们能在严格条件下与本发明的DNA序列杂交或与本发明的致敏原发生交叉免疫反应。
本发明也包括具有降低过敏反应作用的片段、部分序列的新组合和含有点突变的突变体。
本发明因此进一步涉及到编码来自禾本科(poaoaceae)的第4组致敏原的免疫调节、T细胞反应片段的对应的部分序列、部分序列的组合，或含有取代、删除或添加的突变体。
随着对自然发生的致敏原DNA序列的了解，目前，已经有可能制备能够用于过敏性疾病的诊断和治疗的这些致敏原的重组蛋白(Scheiner and Kraft，1995，Allergy 50384-391)。
经典的有效治疗过敏的方法为特异性免疫治疗或脱敏治疗(Fiebig，1995，J.4(6)336-339，Bousquet et 1998，J.Alllergy Clin.Immunol.102(4)558-562)。在此方法中，自然致敏原的提取物以不断提高的剂量给予病人皮下注射。然而这种方法有引起过敏，甚至过敏性休克的危险，为了最大限度地降低这种危险，使用了新型的类致敏原制剂，它们是经过化学修饰的致敏原提取物，其IgE反应性已明显降低，但T细胞反应与未处理的提取物相同(Fiebig，1995，Allergo J.4(7)377-382)。
用重组方法制备的致敏原使更有实质意义的优化治疗成为了可能。通过重组方法获得的且任选性地与病人个体的过敏模式匹配的高纯度的致敏原“鸡尾酒”可以取代自然致敏原来源的提取物，因为除了各种致敏原外，它们含有相对大量的具有免疫原性但无过敏性的二级蛋白。
用表达产物引起可靠的脱敏反应的现实前景因使用经过特异性突变删除了IgE表位，但不损害治疗作用所必需的T细胞表位的重组致敏原而产生(Schramm等，1999，J.Immunol.1622406-2414)。
治疗过敏患者体内TH细胞失衡的另一个可能方法是免疫治疗性DNA疫苗接种，它涉及到用编码相关致敏原的表达性DNA进行治疗。通过给啮齿动物注射致敏原编码DNA已得到致敏原特异性免疫应答影响的初步实验证据(Schramm et 1999，J.Immunol.1622406-2414)。
本发明因此涉及到以上或以下描述的作为药物的DNA分子和作为药物的相应的重组表达载体。
通过重组方法制备的相应的蛋白可以被用于花粉过敏的治疗及体外及体内诊断。
为了制备重组致敏原，克隆的核酸被连接到表达载体上，该构建体在一个适合的宿主生物体内表达，经过生物化学纯化，即可通过已建立的方法用重组致敏原检测IgE抗体。
本发明进一步涉及到含有以上或以下描述的功能性连接到表达控制序列的DNA分子的重组表达载体和被所述DNA分子或所述表达载体转化的宿主生物。
本发明还涉及到至少一种以上描述的DNA分子或至少一种以上描述的表达载体在制备用于禾本科(poaceae)第4组致敏原，优选是小麦族(Triticeae)，尤其是Sec c 4、Hor v 4、Tri a 4参与触发的过敏病人的免疫治疗性DNA药物和/或预防这些过敏的药物中的用途。
正如已经陈述的那样，本发明可以作为重组致敏原或含核酸制剂的基本成分用于特异性免疫治疗，许多种可能在这里呈现，一方面，制剂的组成物可以是一级结构无改变的蛋白；另一方面，根据本发明，可以通过整体特异性删除分子上的IgE表位或产生编码T细胞表位的单一片段产生低致敏(类致敏原)形式来进行治疗以避免不必要的副作用；最后，核酸本身如果被连接到真核表达载体而制成制剂直接使用，能够治疗性地调节过敏免疫状态。
本发明进一步涉及到由以上描述的一个或多个DNA分子编码的多肽，特别是它们作为药物的效用。
它们是具有与SEQ ID NO 2、4、6、8或10一致的氨基酸序列的蛋白，特别值得一提的是它们是成熟蛋白(无信号序列成分)，起始于第23位氨基酸(SEQ ID NO 2、4和6)和第22位氨基酸(SEQ ID NO 8和10)。本发明进一步涉及到含有这些氨基酸序列或这些序列的组成部分的蛋白。
本发明因此也涉及到通过培养宿主生物制备这样的多肽和从培养物中分离相应多肽的方法。
本发明同样涉及到至少一种上述多肽在制备用于诊断和/或治疗禾本科(poaceae)，特别是小麦族(Triticeae)的第4组致敏原，尤其是Secc4、Hor v 4、Tri a 4参与触发的过敏反应的药物和预防这些致敏原的药物中的用途。
在确定本发明的蛋白和DNA序列时，应用了以下步骤
来自黑麦的Sec c 41.从PhI p 4 DNA序列(SEQ ID NO 12，WO04/000881)入手，合成来自PhI p 4序列的特异性引物(表1)，通过PCR用引物#87和#83从黑麦花粉DNA上获得5个克隆，扩增出的对应这些克隆的Sec c 4基因片段1编码与PhI p 4(SEQ ID NO 12)上第68-401位氨基酸序列对应的多肽。
2.用Sec c 4的部分序列在EST数据库进行了检索，在专门的黑麦EST数据库中没有发现与之同源的序列，然而，发现单个的、同源的、非重叠的EST片段在专门的大麦和小麦的EST数据中存在。单个的EST片段延伸至对应基因的5’-UTR区，其他延伸至3’-UTR区(UTR＝非翻译区)。
3.然而，无法从EST数据库中的EST序列拼接出小麦和大麦的完整的第4组基因，因为这些序列不重叠，而且不知道第4组基因的同源序列。但是，参考PhI p 4序列(SEQ ID NO 11)和在步骤1获得的Sec c 4片段可以定位这些EST序列，这些序列可用作制备PCR引物的模板。
4.有了通过这种途径制备的引物#195和#189的帮助，用PCR得到3个克隆，引物#195来自大麦EST序列，引物#189为PhI p 4特异性引物，并与PhI p 4的终止密码子和PhI p 4的C末端的10个氨基酸序列重叠。通过这种途径扩增的Sec c 4基因片段2编码一个起始于信号序列，终止于与PhI p 4对应的490位的多肽。该多肽覆盖Sec c 4的N末端。
5a.用引物#195和#202通过PCR获得了另外3个克隆。这两个引物均来自大麦的EST序列，扩增的Sec c 4基因片段3编码起始于Sec c 4的信号序列，终止于Sec c 4的C末端的氨基酸。由此成熟Secc 4的完整序列以确定的序列被呈现出来。
以下5b和5c两个步骤用于对步骤5a获得的结果的“双保险”确定。5b.通过PCR用引物#195和#203获得了另外3个克隆，引物#195来自大麦的EST序列，引物#203来自小麦EST序列。该扩增的Sec c 4基因编码起始于Sec c 4的信号序列，终止于Sec c 4的C末端的对应氨基酸，由此成熟Sec c 4的完整序列以确定的序列被呈现出来。
5c.用引物#195和#198通过PCR获得了另外1个克隆，(alsoprimer#198)，该扩增的Sec c 4基因编码起始于Sec c 4的信号序列，终止于Sec c 4的C末端的对应氨基酸，由此成熟Sec c 4的完整序列以确定的序列被呈现出来。
发现有Sec c 4.01和Sec c 4.02两种同工型，成熟致敏原起始于对应于SEQ ID NO 2、4和6序列的第23位氨基酸。来自大麦的Hor v 4有通过以上描述获得的Sec c 4序列的帮助，发现同源的EST片段在大麦(Hordeum vulgare)的EST数据库中存在。这些片段重叠、但从中得不出完整基因。参考已发现的EST片段，有可能设计出Horv 4特异性引物，用于从染色体DNA中扩增Hor v 4基因。
总共分析了15个克隆4个克隆用引物#195和#198通过PCR获得。
4个克隆用引物#195和#202通过PCR获得。
3个克隆用引物#194和#198通过PCR获得。
4个克隆用引物#194和#202通过PCR获得。
其衍生的蛋白序列起始于Hor v 4的信号序列并延伸至该蛋白的C末端(从SEQ ID NO 6的第23位氨基酸起)来自小麦的Tri a 4有通过以上描述获得的Sec c 4序列的帮助，发现同源的EST片段在小麦(Triticum aestivum)的EST数据库中存在。这些片段重叠，但从中得不出完整的基因。参考已发现的EST片段，有可能设计出Tria 4特异性引物#199、#203、#204和#206，用于从染色体DNA中扩增Tri a 4基因。
总共分析了13个克隆4个克隆用引物#204和#203通过PCR获得。
4个克隆用引物#204和#199通过PCR获得。
4个克隆用引物#206和#203通过PCR获得。
4个克隆用引物#206和#199通过PCR获得。
其衍生的蛋白序列起始于Tri a 4的信号序列并延伸至该蛋白的C末端。发现有Tri a 4.01(从SEQ ID NO 8的第22位氨基酸起)和Tri a4.02(从SEQ ID NO 10的第22位氨基酸起)两个变异体。
为了制备本发明的重组致敏原，对应于SEQ ID NO 1、3、5、7和9的DNA序列被重组进表达载体(例如pProEx、pSE 380)，应用了E.coli优化密码子来编码从该蛋白序列测定所知的N端氨基酸。
在转化进E.coli后，通过各种分离技术对本发明的重组致敏原进行表达和纯化，得到的蛋白质进行重新折叠。两种致敏原均可用于禾本花粉过敏的高特异性诊断，诊断可以是在体外通过检测特异性抗体(IgE、IgG1-4、IgA)并与携带IgE效应细胞(如血液中的嗜碱性白细胞)反应来进行，也可以是在体内通过皮试反应和在反应器官进行诱发来进行。
通过致敏原特异性刺激T淋巴细胞引起的增殖和细胞因子合成可以检测到禾本花粉过敏患者的T淋巴细胞与本发明的致敏原的反应，细胞可以是新鲜制备的血淋巴细胞中的T细胞，也可以是已经建立的n Sec c 4、nHor v 4或nTri a 4反应性T细胞系和克隆。
对编码半胱氨酸的三联子通过特异性点突变技术进行修饰，使其编码其他氨基酸，优选丝氨酸，制备出了单个半胱氨酸被替代的变异体和那些各种组合的两个半胱氨酸残基或所有半胱氨酸被修饰的变异体，这些半胱氨酸点突变体表达的蛋白与过敏患者的IgE抗体的反应被大大降低或与IgE抗体无反应，但能与这些病人的T淋巴细胞发生反应。
本发明因此涉及到以上或以下描述的DNA分子，这些DNA分子对应的多肽中的一个、多个或所有半胱氨酸残基通过特异性点突变技术被另一个氨基酸所取代。
含有T细胞表位和有低致敏性点突变(例如半胱氨酸取代)的多肽对应的低致敏性片段的免疫调节活性可以通过它们与禾本花粉过敏患者的T细胞进行反应来检测。
这样的低致敏片段或半胱氨酸点突变体可以用作过敏患者的脱敏制剂，因为它们与T细胞的反应效力不变，但由于与IgE的反应性降低或完全缺失所以它们引起的IgE介导的副作用会很低。
如果本发明的编码低致敏性致敏原变异体的核酸或本发明的未经修饰的DNA分子被连接到人体表达载体，这些构建体同样可用作免疫治疗(DNA疫苗接种)制剂。
最后，本发明涉及到用于禾本科(poaceae)，特别是小麦族(Triticeae)的第4组致敏原，尤其是Sec c 4、Hor v 4、Tri a 4参与触发的过敏反应的免疫治疗或预防这些过敏反应的药用组合物，该药用组合物包括至少一种上述DNA分子或至少一种上述表达载体和可选的其他活性成分和/或佐剂。
本发明的另一组适用于所述过敏反应的诊断和/或治疗的药用组合物包含至少一种上述多肽，而不是DNA。
本发明意义上的药用组合物包含作为活性成分的本发明的多肽或表达载体和/或它们各自的药学上适用的衍生物，包括以各种比率混合的它们的混合物。本发明的活性成分可以与至少一种固体、液体和/或半液体赋形剂或佐剂做成合适的剂量形式，并可选择性地与一种或多种其他活性成分联用。
特别合适的佐剂为免疫刺激DNA或含有CpG基序的寡核苷酸。
这些组合物可以用作人类或兽类医疗方面的治疗药物或诊断试剂。合适的赋形剂为适合于肠外给药且对本发明的活性成分的功效没有负面影响的有机或无机物质。适合于肠外使用的，具体有溶液剂，优选油性或水性溶液剂，此外有混悬剂、乳剂或植入剂。本发明的活性成分也可以被冷冻干燥，形成的冻干物用于制备注射制剂。所指组合物可以是灭菌的和/或含有佐剂，如防腐剂、稳定剂和/或润湿剂、乳化剂、调节渗透压的盐、缓冲物质和/或多种其他活性成分。
此外，可以通过相应配制本发明活性成分，例如将其吸附于氢氧化铝而获得缓释制剂。
本发明还作为病人特异性过敏谱的致敏原触发成分鉴别试验的一部分用于改善体外诊断，本发明同样用于禾本花粉过敏的特异性免疫治疗的显著改善制剂的制备。
表1.所用引物a)Sec c 4
b)Hor V 4
c)Tri a 4
序列表<110>默克专利股份公司<120>谷类第4组主要致敏原的DNA序列和重组制品<130>P 03/239<140>DE 10359351.9<141>2003-12-16<160>44<170>PatentIn version 3.1<210>1<211>1603<212>DNA<213>黑麦(Secale cereale)<220>
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<400>1aac tat agg gcc ttc gcg ctg gcg ctc ctc ttc tgc gcc ttg tcc tgc48Asn Tyr Arg Ala Phe Ala Leu Ala Leu Leu Phe Cys Ala Leu Ser Cys15 10 15caa gcc gcc gcg gcc gcc tac gcg ccc gtg cct gcc aag gcg gac ttc 96Gln Ala Ala Ala Ala Ala Tyr Ala Pro Val Pro Ala Lys Ala Asp Phe20 25 30ctc gga tgc ctc atg aag gag ata ccg gcc cgc ctc ctc tac gcc aag144Leu Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys35 40 45agc tcg cct gac tac ccc acc gtg ctg gcg cag acc atc agg aac tcg192Ser Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser50 55 60cgg tgg tcg tcg ccg cag aac gtg aag ccg atctac atc atc acc ccc 240Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Ile Tyr Ile Ile Thr Pro65 70 75 80acc aac gcc tcg cac atc cag tcc gcg gtg gtg tgc ggc cgc cgg cac288Thr Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His85 90 95ggc atc cgc ctc cgc gtg cgg agc ggc ggc cac gactac gag ggc ctg 336Gly Ile Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu100 105 110tcg tac cgg tct gag aaa ccc gag acg ttc gcc gtc gtc gac ctc aac384Ser Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn115 120 125aag atg cgg gca gtg tcg gtc gac ggc tac gcc cgc acg gcg tgg gtc432Lys Met Arg Ala Val Ser Val Asp Gly Tyr Ala Arg Thr Ala Trp Val130 135 140gaa tcc ggc gcg cag ctc ggc gag ctc tac tac gcg atc gcc aag aac480Glu Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn145 150 155 160agc ccc gtg ctc gcg ttc ccg gct ggc gtc tgc ccgtcc atc ggc gtc 528Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val
l65 l70 l75ggc ggc aac ttc gca ggc ggc ggc ttt ggc atg ctg ctg cgc aag tac576Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr180 185 190ggc atc gcc gct gag aac gtc atc gac gtc aag gtg gtc gac ccc aac624Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn195 200 205ggc aag ctg ctc gac aag agc tcc atg agc gcg gac cac ttc tgg gcc672Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala210 215 220gtt agg ggc ggc ggc gga gag agc ttt ggc atc gtc gtc tcg tgg cag720Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln225 230 235 240gtg aag ctc ctg ccg gtg cct ccc acc gtg acc gtg ctc aag atc ccc768Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Leu Lys Ile Pro245 250 255aag acg gtg caa gaa ggc gcc ata gac ctc gtc aac aag tgg cag ctg816Lys Thr Val Gln Glu Gly Ala Ile Asp Leu Val Asn Lys Trp Gln Leu260 265 270gtc ggg ccg gca ctt ccc ggc gac ctc atg atc cgc atc atc ctt gcc864Val Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Ile Ile Leu Ala275 280 285ggg aac agc gcg acg ttc gag gcc atg tac ctg ggc acc tgc agt acc912Gly Asn Ser Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Ser Thr290 295 300ctg acg ccg ctg atg agc agc aaa ttc ccc gag ctt ggc atg aac ccc960Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro305 310 315320tcg cac tgc aac gag atg tcc tgg atc aag tcc atc ccc ttc atc cac1008Ser His Cys Asn Glu Met Ser Trp Ile Lys SerIle Pro Phe Ile His325 330335ctc ggc aag cag aac ctc gac gac ctc ctc aac cgg aac aac acc ttc1056Leu Gly Lys Gln Asn Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe340 345 350aaa cca ttc gcc gaa tac aag tcg gac tac gtg tac cag ccc ttc ccc1104Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro355 360 365aag ccc gtg tgg gag cag atc ttc ggc tgg ctt gtg aag ccc ggc gcg1152Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Val Lys Pro Gly Ala370 375 380ggg atc atg atc atg gac ccc tat ggc gcc acc atc agc gct acc ccc1200Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro385 390 395 400gaa gcg gcg acg ccg ttc cct cac cgc cag ggc gtc ctc ttc aac atc1248Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile405 410 415cag tac gtc aac tac tgg ttc gct gag tca gcc ggc gcg gcg ccg ctg1296
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ser Ala Gly Ala Ala Pro Leu420 425 430cag tgg agc aag gac ata tac aag ttc atg gag ccg tac gtg ago aaa1344Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Tyr Val Ser Lys435 440 445aat ccc agg cag gcg tat gcc aac tac agg gac atc gac ctt ggc agg1392Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg450 455 460aat gag gtg gtg aac gac atc tcc acc tac agc agc ggc aaa gtg tgg1440Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp465 470 475 480ggt gag aag tac ttc aag ggc aac ttc caa agg ctc gcc att acc aag1488Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys485 490 495ggc aag gtg gat cct cag gac tac ttc agg aac gag cag agc atc ccg1536Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro500 505 510cca ctg gtc gag aag tac tga tcgaggacct tgcatggaaa tttagtgcgt 1587Pro Leu Val Glu Lys Tyr515ggttggcgtt tcacat 1603<210>2<211>518<212>PRT<213>黑麦(Secale cereale)<400>2Asn Tyr Arg Ala Phe Ala Leu Ala Leu Leu Phe Cys Ala Leu Ser Cys1 5 10 15Gln Ala Ala Ala Ala Ala Tyr gla Pro Val Pro Ala Lys Ala Asp Phe20 25 30Leu Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys35 40 45Ser Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser50 55 60Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Ile Tyr Ile Ile Thr Pro65 70 75 80Thr Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His
85 90 95Gly Ile Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu100 105 110Ser Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn115 120 125Lys Met Arg Ala Val Ser Val Asp Gly Tyr Ala Arg Thr Ala Trp Val130 135 140Glu Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn145 150 155 160Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val165 170 175Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr180 185 190Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn195 200 205Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala210 215 220Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln225 230 235 240Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Leu Lys Ile Pro245 250 255Lys Thr Val Gln Glu Gly Ala Ile Asp Leu Val Asn Lys Trp Gln Leu260 265 270Val Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Ile Ile Leu Ala275 280 285Gly Asn Ser Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Ser Thr290 295 300Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro305 310 315 320Ser His Cys Asn Glu Met Ser Trp Ile Lys Ser Ile Pro Phe Ile His325 330 335
Leu Gly Lys Gln Asn Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe340 345 350Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro355 360 365Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Val Lys Pro Gly Ala370 375 380Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro385 390 395 400Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile405 410 415Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ser Ala Gly Ala Ala Pro Leu420 425 430Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Tyr Val Ser Lys435 440 445Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg450 455 460Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp465 470 475 480Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys485 490 495Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro500 505 510Pro Leu Val Glu Lys Tyr515<210>3<211>1644<212>DNA<213>黑麦(Secale cereale)<220>
<221>stop_codon
<222>(1561)..(1563)<223>
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<221>signal_sequence_DNA<222>(1)..(66)<223>
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<400>3aac tcg agg gcc ttt gct ctg gtg ccc ctc ctc atc tgc gtc ttg tcc48Asn Ser Arg Ala Phe Ala Leu Val Pro Leu Leu Ile Cys Val Leu Ser1 5 10 15tgc cac gcc gcc gtc tcc tac gcg gcg gcg ccg gtg ccg gcc aag gag96Cys His Ala Ala Val Ser Tyr Ala Ala Ala Pro Val Pro Ala Lys Glu20 25 30gac ttc ttc gga tgc ctg gtg aag gag ata ccg gcc cgc ctc ctc tac144Asp Phe Phe Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Tyr35 40 45gcc aag agc tcg cct gcc ttc ccc acc gtc ctg gcg cag acc atc agg192Ala Lys Ser Ser Pro Ala Phe Pro Thr Val Leu Ala Gln Thr Ile Arg50 55 60aac tcg cgg tgg tcg tcg ccg cag agc gtg aag ccg ctc tac atc atc240Asn Ser Arg Trp Ser Ser Pro Gln Ser Val Lys Pro Leu Tyr Ile Ile65 70 75 80acc ccc acc aac gcc tcc cac atc cag tcc gcg gtg gtg tgc ggc cgc288Thr Pro Thr Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg85 90 95cgg cac ggc gtc cgc atc cgc gtg cgg agc ggc ggc cac gac tac gag 336Arg His Gly Val Arg Ile Arg Val Arg Ser Gly Gly His Asp Tyr Glu
100 105 110ggc ctg tcg tac cgg tcc gag cgc ccc gag gcg ttc gcc gtc gtc gac384Gly Leu Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp115 120 125ctc aac aag atg cgg gcc gtg gtg gtc gac ggc aag gct cgc acg gcg 432Leu Asn Lys Met Arg Ala Val Val Val Asp Gly Lys Ala Arg Thr Ala130 135 140tgg gtg gac tcc ggt gcg cag ctc ggc gag ctc tac tac gcc atc gcc 480Trp Val Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala145 150 155 160aag aac agc ccc gtg ctc gcg ttc ccg gcc ggc gtt tgc ccg acc att528Lys Asn Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile165 170 175ggt gta ggc ggc aac ttc gct ggc ggc ggc ttc ggc atg ctg ctg cgc576Gly Val Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg180 185 190aag tac ggc atc gcc gcc gag aac gtc atc gac gtg aag gtg gtc gac624Lys Tyr Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp195 200 205gcc aac ggc aca ctg ctc gac aag agc tco atg agc gcg gat cac ttc 672Ala Asn Gly Thr Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe210 215 220tgg gcc gtc agg ggc ggc ggc gga gag agc ttc ggc atc gtc gtg tcg 720Trp Ala Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser225 230 235 240tgg cag gtg aag ctc ctc ccg gtg cct ccc acc gtg acc gtg ttc aag768Trp Gln Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys245 250 255atc ccc aag acg gtg caa gaa ggc gcc gta gag ctc atc aac aag tgg816Ile Pro Lys Thr Val Gln Glu Gly Ala Val Glu Leu Ile Asn Lys Trp260 265 270cag cta gtc gcg ccg gcc ctc ccc gac gac ctg atg atc cgc atc atc864Gln Leu Val Ala Pro Ala Leu Pro Asp Asp Leu Met Ile Arg Ile Ile275 280 285gct ttc ggc ggc acc gcc aag ttc gag gcc atg tac ctg ggc acc tgc912Ala Phe Gly Gly Thr Ala Lys Phe Glu Ala Met Tyr Leu Gly Thr Cys290 295 300aaa gcc ctg aca ccg ctg atg agc agc aga ttc ccc gag ctc ggc atg960Lys Ala Leu Thr Pro Leu Met Ser Ser Arg Phe Pro Glu Leu Gly Met305 310 315 320aac gcc tcg cac tgc aac gag atg ccc tgg atc aag tcc gtc cca ttc1008Asn Ala Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe325 330 335atc cac cttggc aag cag gcc acc ctc tcc gac ctc ctc aac cgg aac 1056Ile His Leu Gly Lys Gln Ala Thr Leu Ser Asp Leu Leu Asn Arg Asn340 345 350aac acc ttc aaa ccc ttc gcc gag tac aag tcg gac tac gtc tac cag1104
Asn Thr Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln355 360 365ccc gtc ccc aag ccc gtc tgg gcg cag atc ttc gtc tgg ctc gtc aaa1152Pro Val Pro Lys Pro Val Trp Ala Gln Ile Phe Val Trp Leu Val Lys370 375 380ccc ggc gcc ggg atc atg gtc atg gac ccc tac ggc gcc gcc atc agc1200Pro Gly Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Ala Ile Ser385 390 395 400gcc acc ccc gaa gcc gcc acg ccg ttc cct cac cgc aag gac gtc ctc1248Ala Thr Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Asp Val Leu405 410 415ttc aac atc cag tac gtc aac tac tgg ttc gac gag gca ggc ggc gcc1296Phe Asn Ile Gln Tyr Val Asn Tyr Trp Phe Asp Glu Ala Gly Gly Ala420 425 430gcg ccg ctg cag tgg agc aag gac atg tac agg ttc atg gag ccg tac1344Ala Pro Leu Gln Trp Ser Lys Asp Met Tyr Arg Phe Met Glu Pro Tyr435 440 445gtc agc aag aac ccc aga cag gcc tac gcc aac tac agg gac atc gac1392Val Ser Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp450 455 460ctc ggc agg aac gag gtg gtc aac gac atc tcc acc tat gcc agc ggc1440Leu Gly Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ala Ser Gly465 470 475 480aag gtc tgg ggc gag aag tac ttc aag ggc aac ttc caa agg ctc gcc1488Lys Val Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala485 490 495att acc aag ggc aag gtg gat cct cag gac tac ttc agg aac gag cag1536Ile Thr Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln500 505 510agc atc ccg ccg ctg cta ggg aag tag tagtactctt gcttgcatgg 1583Ser Ile Pro Pro Leu Leu Gly Lys515 520agatttgtag tgcgtctttc gcgtttcaaa tgcccaacta gtagaataag gatcgtgcgt 1643a 1644<210>4<211>520<212>PRT<213>黑麦(Secale cereale)<400>4Asn Ser Arg Ala Phe Ala Leu Val Pro Leu Leu Ile Cys Val Leu Ser1 5 10 15
Cys His Ala Ala Val Ser Tyr Ala Ala Ala Pro Val Pro Ala Lys Glu20 25 30Asp Phe Phe Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Tyr35 40 45Ala Lys Ser Ser Pro Ala Phe Pro Thr Val Leu Ala Gln Thr Ile Arg50 55 60Asn Ser Arg Trp Ser Ser Pro Gln Ser Val Lys Pro Leu Tyr Ile Ile65 70 75 80Thr Pro Thr Asn Ala Ser His Ile Gln Ser ALa Val Val Cys Gly Arg85 90 95Arg His Gly Val Arg Ile Arg Val Arg Ser Gly Gly His Asp Tyr Glu100 105 110GlV Leu Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp115 120 125Leu Asn Lys Met Arg Ala Val Val Val Asp Gly Lys Ala Arg Thr Ala130 135 140Trp Val Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala145 150 155 160Lys Asn Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile165 170 175Gly Val Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg180 185 190Lys Tyr Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp195 200 205Ala Asn Gly Thr Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe210 215 220Trp Ala Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser225 230 235 240Trp Gln Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys245 250 255Ile Pro Lys Thr Val Gln Glu Gly Ala Val Glu Leu Ile Asn Lys Trp260 265 270
Gln Leu Val Ala Pro Ala Leu Pro Asp Asp Leu Met Ile Arg Ile Ile275 280 285Ala Phe Gly Gly Thr Ala Lys Phe Glu Ala Met Tyr Leu Gly Thr Cys290 295 300Lys Ala Leu Thr Pro Leu Met Ser Ser Arg Phe Pro Glu Leu Gly Met305 310 315 320Asn Ala Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe325 330 335Ile His Leu Gly Lys Gln Ala Thr Leu Ser Asp Leu Leu Asn Arg Asn340 345 350Asn Thr Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln355 360 365Pro Val Pro Lys Pro Val Trp Ala Gln Ile Phe Val Trp Leu Val Lys370 375 380Pro Gly Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Ala Ile Ser385 390 395 400Ala Thr Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Asp Val Leu405 410 415Phe Asn Ile Gln Tyr Val Asn Tyr Trp Phe Asp Glu Ala Gly Gly Ala420 425 430Ala Pro Leu Gln Trp Ser Lys Asp Met Tyr Arg Phe Met Glu Pro Tyr435 440 445Val Ser Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp450 455 460Leu Gly Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ala Ser Gly465 470 475 480Lys Val Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala485 490 495Ile Thr Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln500 505 510Ser Ile Pro Pro Leu Leu Gly Lys
515 520<210>5<211>1608<212>DNA<213>大麦(Hordeum vulgare)<220>
<221>stop_codon<222>(1555)..(1557)<223>
<220>
<221>signal_sequence_DNA<222>(1)..(66)<223>
<220>
<221>signal_sequence_PROT<222>(1)..(22)<223>
<220>
<221>CDS<222>(1)..(1557)<223>
<400>5agc tcg agg gcc ttc gct ctg gtg ctc ctc ctc tgc gcc ttg tcc tgc48Ser Ser Arg Ala Phe Ala Leu Val Leu Leu Leu Cys Ala Leu Ser Cys1 5 10 15cac cac gct gcc gtc tcc tcc gcg cag gtg ccg gcc aag gac gac ttc96His His Ala Ala Val Ser Ser Ala Gln Val Pro Ala Lys Asp Asp Phe20 25 30ctg gga tgc ctc gtg aag gag ata ccg gcc cgc ctc ctc ttc gcc aag144
Leu Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Phe Ala Lys35 40 45agc tcg cct gcc ttc ccc gcc gtc ctg gag cag acc atc agg aac tcg 192Ser Ser Pro Ala Phe Pro Ala Val Leu Glu Gln Thr Ile Arg Asn Ser50 55 60cgg tgg tcg tcg ccg cag aac gtg aag ccg ctc tac atc atc acc ccc 240Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro65 70 75 80acc aac acc tcc cac atc cag tct gct gtg gtg tgc ggc cgc cgg cac 288Thr Asn Thr Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His85 90 95ggc gtc cgc ctc cgc gtg cgg agc ggc ggc cac gac tac gag ggc ctg 336Gly Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu100 105 110tcg tac cgg tcc gag cgc ccc gag gcg ttc gcc gtc gta gac ctc aac 384Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp Leu Asn115 120 125aag atg cgg acc gtg ttg gtc aac gaa aag gcc cgc acg gcg tgg gtg 432Lys Met Arg Thr Val Leu Val Asn Glu Lys Ala Arg Thr Ala Trp Val130 135 140gac tcc ggc gcg cag ctc ggc gag ctc tac tac gcc atc gcc aag aac 480Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn145 150 155 160agc ccc gtg ctc gcg ttc cca gcc ggc gtt tgc ccg tcc att ggt gta 528Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val165 170 175ggt ggc aac ttc gct ggc ggc ggc ttc ggc atg ctg ctg cgc aag tac 576Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr180 185 190ggc atc gcc gcc gag aac gtc atc gac gtc aag ctg gtc gac gcc aac 624Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn195 200 205ggc aag ctg ctc gac aag agc tcc atg agc ccg gac cac ttc tgg gcc 672Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala210 215 220gtc agg ggc ggc ggc gga gag agc ttc ggc atc gtc gtc tcg tgg cag 720Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln225 230 235 240gtg aag ctt ctc ccg gtg cct ccc acc gtg act gtg ttt cag atc ccc 768Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Gln Ile Pro245 250 255aag aca gtg caa gaa ggc gcc gta gac ctc atc aac aag tgg cag ctg 816Lys Thr Val Gln Glu Gly Ala Val Asp Leu Ile Asn Lys Trp Gln Leu260 265 270gtc gcg ccg gcc ctt ccc ggc gac atc atg atc cgc atc atc gcc atg864Val Ala Pro Ala Leu Pro Gly Asp Ile Met Ile Arg Ile Ile Ala Met275 280 285
ggg gac aaa gcg acg ttc gag gcc atg tac ctg ggc acc tgc aaa acc912Gly Asp Lys Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr290 295 300ctg acg ccg ctg atg agc agc aaa ttc ccg gag ctt ggc atg aac cco960Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro305 310 315 320tcg cac tgc aac gag atg ccc tgg atc aag tcc atc ccc ttc atc cac1008Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His325 330 335ctt ggc aag cag gcc acc ctg gcc gac ctc ctc aac cgg aac aac acc1056Leu Gly Lys Gln Ala Thr Leu Ala Asp Leu Leu Asn Arg Asn Asn Thr340 345 350ttc aaa ccc ttc gcc gaa tac aag tcg gac tac gtc tac cag ccc gtc1104Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Val355 360 365ccc aag ccc gtg tgg gag cag ctc ttc ggc tgg ctc acg aaa ccc ggc1152Pro Lys Pro Val Trp Glu Gln Leu Phe Gly Trp Leu Thr Lys Pro Gly370 375 380gcg ggg atc atg gtc atg gac cca tac ggc gcc acc atc agc gcc acc1200Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr385 390 395 400ccc gaa gcg gcg acg ccg ttc cct cac cgc aag ggc gtc ctc ttc aac1248Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn405 410 415atc cag tac gtc aac tac tgg ttc gcc gag gca gcc ggc gcc gcg ccg1296Ile Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Gly Ala Ala Pro420 425 430ctg cag tgg agc aag gac att tac aaa ttc atg gag ccg ttc gtg agc1344Leu Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Phe Val Ser435 440 445aag aac ccc agg cag gcg tac gcc aac tac agg gsc atc gac ctc ggc1392Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly450 455 460agg aac gag gtg gtg aac gac atc tca acc tac agc agc ggc aag gtg1440Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val465 470 475 480tgg ggc gag aag tac ttc aag ggc aac ttc caa agg ctc gcc atc acc1488Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr485 490 495aag ggc aag gtg gat ccc cag gac tac ttc agg aac gag cag agc atc1536Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile500 505 510ccg ccg ctg ctg ggc aag tag tgaccgagag tcttgcatgg agatttgtag 1587Pro Pro Leu Leu Gly Lys515tgcgtgcttg gcgtttctga t1608
<210>6<211>518<212>PRT<213>大麦(Hordeum vulgare)<400>6Ser Ser Arg Ala Phe Ala Leu Val Leu Leu Leu Cys Ala Leu Ser Cys1 5 10 15His His Ala Ala Val Ser Ser Ala Gln Val Pro Ala Lys Asp Asp Phe20 25 30Leu Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Phe Ala Lys35 40 45Ser Ser Pro Ala Phe Pro Ala Val Leu Glu Gln Thr Ile Arg Asn Ser50 55 60Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro65 70 75 80Thr Asn Thr Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His85 90 95Gly Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu100 105 110Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp Leu Asn115 120 125Lys Met Arg Thr Val Leu Val Asn Glu Lys Ala Arg Thr Ala Trp Val130 135 140Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn145 150 155 160Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val165 170 175Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr180 185 190Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn195 200 205
Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala210 215 220Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln225 230 235 240Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Gln Ile Pro245 250 255Lys Thr Val Gln Glu Gly Ala Val Asp Leu Ile Asn Lys Trp Gln Leu260 265 270Val Ala Pro Ala Leu Pro Gly Asp Ile Met Ile Arg Ile Ile Ala Met275 280 285Gly Asp Lys Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr290 295 300Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro305 310 315 320Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His325 330 335Leu Gly Lys Gln Ala Thr Leu Ala Asp Leu Leu Asn Arg Asn Asn Thr340 345 350Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Val355 360 365Pro Lys Pro Val Trp Glu Gln Leu Phe Gly Trp Leu Thr Lys Pro Gly370 375 380Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr385 390 395 400Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn405 410 415Ile Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Gly Ala Ala Pro420 425 430Leu Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Phe Val Ser435 440 445Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly450 455 460
Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val465 470 475 480Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr485 490 495Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile500 505 510Pro Pro Leu Leu Gly Lys515<210>7<211>1603<212>DNA<213>小麦(Triticum aestivum)<220>
<221>stop_codon<222>(1555)..(1557)<223>
<220>
<221>signal_sequence_DNA<222>(1)..(63)<223>
<220>
<221>signal_sequence_PROT<222>(1)..(21)<223>
<220>
<221>CDS
<222>(1)..(1557)<223>
<400>7aac tat agg gcc ttc acg ctg gtg ctc ctc ttc tgc gcc ttg tcc tgt48Asn Tyr Arg Ala Phe Thr Leu Val Leu Leu Phe Cys Ala Leu Ser Cys1 5 10 15caa gcc gcc gcc acc tac gcg ccg gtg cct gcc aag gag gac ttc ctc96Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu20 25 30gga tgc ctc atg aag gag ata ccg gca cgc ctc ctc tac gcc aag agc144Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser35 40 45tcg cct gacttc ccc acc gtc ctg gcg cag acc atc agg aac tcg cgg 192Ser Pro Asp Phe Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg50 55 60tgg ttg tcg ccg cag aac gtg aag ccg ctc tac atc atc acc ccc acc240Trp Leu Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr65 70 75 80aac gcc tcg cac atc cag tcc gcg gtg gtg tgc gga cgc cgg cac agc288Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser85 90 95gtc cgc ctc cgc gtc cgg agc ggc ggc cac gac tac gag ggc ctg tcg336Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser100 105 110tac cgg tcc gag aaa ccc gag acg ttc gcc gtc gtc gac ctc aac aag384Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys115 120 125atg cgg gca gtg ttg atc gac ggc tac gcc cgc acg gcg tgg gtc gaa432Met Arg Ala Val Leu Ile Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu130 135 140tcc ggc gcg cag ctc ggc gag ctc tac tac gcc atc gcg aaa aac agc480Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser145 150 155 160ccc gtg ctc gcg ttc ccg gcc ggc gtc tgc ccg acc atc ggc gtc ggc528Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly165 170 175ggc aac ttc gca ggc ggc ggc ttt ggc atg ctg ctg cgg aag tac ggc576Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly180 185 190atc gcc gcc gag aac gtc atc gac gtc aag gtg gtc gac ccc aac ggc624Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn Gly195 200 205aag ctt ctc gac aag agc tcc atg agc ccg gac cac ttc tgg gcc gtc672Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala Val210 215 220
agg ggc ggc ggc gga gag agc ttt ggc atc gtc gtg tcg tgg caa gtg720Arg Gly Gly Gly Gly Glu Ser Phe GlyIle Val Val Ser Trp Gln Val225 230235 240aag ctc ctg ccg gtg cct ccc acc gtg acc gtg ttc aag atc ccc aag768Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys245 250 255aca gtg caa gaa ggc gcc gta gac ctc gtc aac aag tgg caa ctg gtc816Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val260 265 270ggg ccg gcc ctt ccc ggc gac ctc atg atc cgc gtc atc gct gcg ggg864Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly275 280 285aac acc gcg aca ttc gag ggc atg tac ctg ggc acc tgc caa acc ctg912Asn Thr Ala Thr Phe Glu Gly Met Tyr Leu Gly Thr Cys Gln Thr Leu290 295 300acg ccg ttg atg agc agc caa ttc ccc gag ctt ggc atg aac ccc tat960Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr305 310 315 320cac tgc aac gag atg ccc tgg atc aag tcc atc ccc ttc atc cac ctc1008His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His Leu325 330 335ggc aaa gag gcc agc ctg gtc gac ctc ctc aac cgg aac aac acc ttc1056Gly Lys Glu Ala Ser Leu Val Asp Leu Leu Asn Arg Asn Asn Thr Phe340 345 350aag ccc ttc gcc gaa tac aag tcg gac tac gtg tac cag ccc ttc ccc1104Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro355 360 365aag ccc gtg tgg gag cag atc ttc ggc tgg ctc acg aag ccc ggt ggg1152Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Thr Lys Pro Gly Gly370 375 380ggg atg atg atc atg gac cca tac ggc gcc acc atc agc gcc acc ccc1200Gly Met Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro385 390 395 400gaa gcg gcg acg ccg ttc cct cac cgc cag ggc gtt ctc ttc aao atc1248Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile405 410 415cag tac gtc aac tac tgg ttc gcc gag gca gcc gcc gcc gcg ccg ctg1296Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Ala Ala Ala Pro Leu420 425 430cag tgg agc aag gac atg tac aat ttc atg gag ccg tac gtg agc aag1344Gln Trp Ser Lys Asp Met Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys435 440 445aac ccc agg cag gcg tac gcc aac tac agg gac att gac ctc ggc agg1392Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg450 455 460aac gag gtg gtg aac gac atc tca acc tat agc agc ggc aag gtt tgg1440Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp465 470 475 480
ggc gag aag tac ttc aag ggc aac ttc caa agg ctc gct att acc aag1488Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys485 490 495ggc aag gtg gat cct cag gac tac ttc agg aac gag cag agc atc ccg1536Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro500 505 510ccg ctg ctc gag aag tac tga tcgaggacct tgcatggaga tttagtgcgt 1587Pro Leu Leu Glu Lys Tyr515ggttgccgtt tcacat 1603<210>8<211>518<212>PRT<213>小麦(Triticum aestivum)<400>8Asn Tyr Arg Ala Phe Thr Leu Val Leu Leu Phe Cys Ala Leu Ser Cys1 5 10 15Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu20 25 30Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser35 40 45Ser Pro Asp Phe Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg50 55 60Trp Leu Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr65 70 75 80Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser85 90 95Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser100 105 110Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys115 120 125Met Arg Ala Val Leu Ile Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu130 135 140
Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser145 150 155 160Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly165 170 175Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly180 185 190Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn Gly195 200 205Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala Val210 215 220Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln Val225 230 235 240Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys245 250 255Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val260 265 270Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly275 280 285Asn Thr Ala Thr Phe Glu Gly Met Tyr Leu Gly Thr Cys Gln Thr Leu290 295 300Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr305 310 315 320His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His Leu325 330 335Gly Lys Glu Ala Ser Leu Val Asp Leu Leu Asn Arg Asn Asn Thr Phe340 345 350Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro355 360 365Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Thr Lys Pro Gly Gly370 375 380Gly Met Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro385 390 395 400
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile405 410 415Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Ala Ala Ala Pro Leu420 425 430Gln Trp Ser Lys Asp Met Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys435 440 445Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg450 455 460Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp465 470 475 480Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys485 490 495Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro500 505 510Pro Leu Leu Glu Lys Tyr515<210>9<211>1603<212>DNA<213>小麦(Triticum aestivum)<220>
<221>stop_Codon<222>(1555)..(1557)<223>
<220>
<221>CDS<222>(1)..(1557)<223>
<220>
<221>signal_sequence_DNA<222>(1)..(63)<223>
<220>
<221>signal_sequence_PROT<222>(1)..(21)<223>
<400>9aac tgt agg gcc ttc gcg cag gtg ctc ctc ttc ttc gcc ttg tcc tgc48Asn Cys Arg Ala Phe Ala Gln Val Leu Leu Phe Phe Ala Leu Ser Cys1 5 10 15caa gcc gcc gcc acc tac gcg ccg gtg cct gcc aag gag gac ttc ctc96Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu20 25 30gga tgc ctc atg aag gag ata ccg gcc cgc ctc ctc tac gcc aag agc144Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser35 40 45tcg cctgac tac ccc acc gtg ctg gcg cag acc atc agg aac tcg cgg 192Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg50 55 60tgg tcg acg cag cag aac gtg aag ccg ctg tac atc atc acc ccc acc240Trp Ser Thr Gln Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr65 70 75 80aac gcc tcc cac atc caa tcc gcg gtg gtg tgc ggc cgc cgg cac ggc288Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Gly85 90 95gtc cgc ctc cgc gtg cgg agc ggc ggc cac gac tac gag ggc ctg tcg336Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser100 105 110tac cgg tcc gag aaa ccc gag acg ttc gcc gtc gtc gac ctc aac aag384Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys115 120 125atg cgg gca gtg gtt gtc gac ggc tac gcc cgc acg gcg tgg gtc gaa432Met Arg Ala Val Val Val Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu130 135 140tcc ggc gcg cag ctc ggc gag ctc tac tac gcc atc gcg aag aac agc480Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser145 150 155 160
ccc gtg ctc gcg ttc ccg gcc ggc gtc tgc ccg tcc atc ggc gtc ggc528Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val Gly165 170 175ggc aac ttc gca ggc ggc ggc ttc ggc atg ctg ctg cgc aag tac ggc576Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly180 185 190atc gcc gcc gag aac gtc atc gac gtc aag gtg gtc gac ccc gac ggc624Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asp Gly195 200 205aag ctg ctc gac aag agc tcc atg agc gcg gac cac ttc tgg gcc gtc672Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala Val210 215 220agg ggc ggc ggc gga gag agc ttc ggc atc gtc gtc tcg tgg cag gtg720Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln Val225 230 235 240aag ctc atg cca gtg cct ccc acc gtc acc gtg ttt aag atc ccc aag768Lys Leu Met Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys245 250 255acg gtg caa gaa ggc gcc gta gac ctc gtc aac aag tgg cag ctg gtc816Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val260 265 270ggg ccg gca ctt ccc ggc gac ctc atg atc cgc gtc atc gct gcc ggg864Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly275 280 285aac acg 9cg acg ttc gag gcc ttg tac ctg ggc acc tgc aaa acc ctg912Asn Thr Ala Thr Phe Glu Ala Leu Tyr Leu Gly Thr Cys Lys Thr Leu290 295 300acg ccg ctg atg agc agc caa ttc ccc gag ctt ggc atg aac ccc tat960Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr305 310 315 320cac tgc aac gag atg ccc tgg atc aag tcc gtc ccc ttc atc cac ctc1008His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe Ile His Leu325 330 335ggc aaa cag gct ggc ctg gac gac ctc ctc aac cgg aac aac acc ttc1056Gly Lys Gln Ala Gly Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe340 345 350aag ccc ttc gcc gaa tac aag tcg gac tac gtg tac cag ccc ttc ccc1104Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro355 360 365aag ccc gtg tgg gag cag atc ttc ggc tgg ctc gcg aag ccc ggc gcg1152Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Ala Lys Pro Gly Ala370 375 380ggg atc atg atc atg gac ccc tac ggc gcc acc atc agc gcc acc ccc1200Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro385 390 395 400gaa gcg gcg acg ccg ttc cct cac cgc cag ggc gtc ctc ttc aac atc1248Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile405 410 415
cag tat gtc aac tac tgg ttc gcc gag cca gcc ggc gcc gcg ccg ctg1296Gln Tyr Val Asn Tyr Trp Phe Ala Glu Pro Ala Gly Ala Ala Pro Leu420 425 430cag tgg agc aag gac att tac aat ttc atg gag ccg tac gtg agc aag1344Gln Trp Ser Lys Asp Ile Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys435 440 445aac ccc agg cag gcg tac gcc aac tac agg gac atc gac ctc ggc agg1392Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg450 455 460aat gag gtg gtg aac gac atc tca acc tac agc agc ggc aag gtg tgg1440Asn Glu ValVal Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp465470 475 480ggc gag aag tac ttc aag agc aac ttc caa agg ctc gcc att acc aag1488Gly Glu Lys Tyr Phe Lys Ser Asn Phe Gln Arg Leu Ala Ile Thr Lys485 490 495ggc aag gta gat cct cag gac tac ttc agg aat gag caa agc atc ccg1536Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro500 505 510ccg ctg atc gag aag tac tga tcgaggacct tgcatggaga tttagtgcgt 1587Pro Leu Ile Glu Lys Tyr515ggttggcgtt tcacat 1603<210>10<211>518<212>PRT<213>小麦(Triticum aestivum)<400>10Asn Cys Arg Ala Phe Ala Gln Val Leu Leu Phe Phe Ala Leu Ser Cys1 5 10 15Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu20 25 30Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser35 40 45Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg50 55 60Trp Ser Thr Gln Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr65 70 75 80
Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Gly85 90 95Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser100 105 110Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys115 120 125Met Arg Ala Val Val Val Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu130 135 140Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser145 150 155 160Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val Gly165 170 175Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly180 185 190Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asp Gly195 200 205Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala Val210 215 220Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln Val225 230 235 240Lys Leu Met Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys245 250 255Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val260 265 270Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly275 280 285Asn Thr Ala Thr Phe Glu Ala Leu Tyr Leu Gly Thr Cys Lys Thr Leu290 295 300Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr305 310 315 320His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe Ile His Leu325 330 335
Gly Lys Gln Ala Gly Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe340 345 350Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro355 360 365Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Ala Lys Pro Gly Ala370 375 380Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro385 390 395 400Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile405 410 415Gln Tyr Val Asn Tyr Trp Phe Ala Glu Pro Ala Gly Ala Ala Pro Leu420 425 430Gln Trp Ser Lys Asp Ile Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys435 440 445Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg450 455 460Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp465 470 475 480Gly Glu Lys Tyr Phe Lys Ser Asn Phe Gln Arg Leu Ala Ile Thr Lys485 490 495Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro500 505 510Pro Leu Ile Glu Lys Tyr515<210>11<211>1503<212>DNA<213>梯牧草(Phleum pratense)<220>
<221>CDS<222>(1)..(1503)<223>
<400>11tac ttc ccg ccg ccg gct gct aaa gaa gac ttc ctg ggt tgc ctg gtt48Tyr Phe Pro Pro Pro Ala Ala Lys Glu Asp Phe Leu Gly Cys Leu Val1 5 10 15aaa gaa atc ccg ccg cgt ctg ttg tac gcg aaa tcg tcg ccg gcg tat96Lys Glu Ile Pro Pro Arg Leu Leu Tyr Ala Lys Ser Ser Pro Ala Tyr20 25 30ccc tca gtc ctg ggg cag acc atc cgg aac tcg agg tgg tcg tcg ccg144Pro Ser Val Leu Gly Gln Thr Ile Arg Asn Ser Arg Trp Ser Ser Pro35 40 45gac aac gtg aag ccg ctc tac atc atc acc ccc acc aac gtc tcc cac192Asp Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr Asn Val Ser His50 55 60atc cag tcc gcc gtg gtg tgc ggc cgc cgc cac agc gtc cgc atc cgc240Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser Val Arg Ile Arg65 70 75 80gtg cgc agc ggc ggg cac gac tac gag ggc ctc tcg tac cgg tct ttg288Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Arg Ser Leu85 90 95cag ccc gag acg ttc gcc gtc gtc gac ctc aac aag atg cgg gcg gtg336Gln Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys Met Arg Ala Val100 105 110tgg gtg gac ggc aag gcc cgc acg gcg tgg gtg gac tcc ggc gcg cag384Trp Val Asp Gly Lys Ala Arg Thr Ala Trp Val Asp Ser Gly Ala Gln115 120 125ctc ggc gag ctc tac tac gcc atc tat aag gcg agc ccc acg ctg gcg432Leu Gly Glu Leu Tyr Tyr Ala Ile Tyr Lys Ala Ser Pro Thr Leu Ala130 135 140ttc ccg gcc ggc gtg tgc ccg acg atc gga gtg ggc ggc aac ttc gcg480Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly Gly Asn Phe Ala145 150 155 l60ggc ggc ggc ttc ggc atg ctg ctg cgc aag tac ggc atc gcc gcg gag528Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly Ile Ala Ala Glu165 170 175aac gtc atc gac gtg aag ctc gtc gac gcc aac ggc aag ctg cac gac576Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn Gly Lys Leu His Asp180 185 190aag aag tcc atg ggc gac gac cat ttc tgg gcc gtc agg ggc ggc ggg624Lys Lys Ser Met Gly Asp Asp His Phe Trp Ala Val Arg Gly Gly Gly195 200 205ggc gag agc ttc ggc ate gtg gtc gcg tgg cag gtg aag ctc ctg ccg672Gly Glu Ser Phe Gly Ile Val Val Ala Trp Gln Val Lys Leu Leu Pro
210 215 220gtg ccg ccc acc gtg aca ata ttc aag atc tcc aag aca gtg agc gag720Val Pro Pro Thr Val Thr Ile Phe Lys Ile Ser Lys Thr Val Ser Glu225 230 235 240ggc gcc gtg gac atc atc aac aag tgg caa gtg gtc gcg ccg cag ctt768Gly Ala Val Asp Ile Ile Asn Lys Trp Gln Val Val Ala Pro Gln Leu245 250 255ccc gcc gac ctc atg atc cgc atc atc gcg cag ggg ccc aag gcc acg816Pro Ala Asp Leu Met Ile Arg Ile Ile Ala Gln Gly Pro Lys Ala Thr260 265 270ttc gag gcc atg tac ctc ggc acc tgc aaa acc ctg acg ccg ttg atg864Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr Leu Thr Pro Leu Met275 280 285agc agc aag ttc ccg gag ctc ggc atg aac ccc tcc cac tgc aac gag912Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro Ser His Cys Asn Glu290 295 300atg tca tgg atc cag tcc atc ccc ttc gtc cac ctc ggc cac agg gac960Met Ser Trp Ile Gln Ser Ile Pro Phe Val His Leu Gly His Arg Asp305 3l0 315 320gcc ctc gag gac gac ctc ctc aac cgg aac aac tcc ttc aag ccc ttc1008Ala Leu Glu Asp Asp Leu Leu Asn Arg Asn Asn Ser Phe Lys Pro Phe325 330 335gcc gaa tac aag tcc gac tac gtc tac cag ccc ttc ccc aag acc gtc1056Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro Lys Thr Val340 345 350tgg gag cag atc ctc aac acc tgg ctc gtc aag ccc ggc gcc ggg atc1104Trp Glu Gln Ile Leu Asn Thr Trp Leu Val Lys Pro Gly Ala Gly Ile355 360 365atg atc ttc gac ccc tac ggc gcc acc atc agc gcc acc ccg gag tcc1152Met Ile Phe Asp Pro Tyr Gly Ala Thr Ile Ssr Ala Thr Pro Glu Ser370 375 380gcc acg ccc ttc cct cac cgc aag ggc gtc ctc ttc aac atc cag tac1200Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn Ile Gln Tyr385 390 395 400gtc aac tac tgg ttc gcc ccg gga gcc gcc gcc gcg ccc ctc tcg tgg1248Val Asn Tyr Trp Phe Ala Pro Gly Ala Ala Ala Ala Pro Leu Ser Trp405 410 415agc aag gac atc tac aac tac atg gag ccc tac gtg agc aag aac ccc1296Ser Lys Asp Ile Tyr Asn Tyr Met Glu Pro Tyr Val Ser Lys Asn Pro420 425 430agg cag gcg tac gca aac tac agg gac atc gac ctc ggc agg aac gag1344Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg Asn Glu435 440 445gtg gtc aac gac gtc tcc acc tac gcc agc ggc aag gtc tgg ggc cag1392Val Val Asn Asp Val Ser Thr Tyr Ala Ser Gly Lys Val Trp Gly Gln450 455 460aaa tac ttc aag ggc aac ttc gag agg ctc gcc att acc aag ggc aag1440
Lys Tyr Phe Lys Gly Asn Phe Glu Arg Leu Ala Ile Thr Lys Gly Lys465 470 475 480gtc gat cct acc gac tac ttc agg aac gag cag agc atc ccg ccg ctc1488Val Asp Pro Thr Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro Pro Leu485 490 495atc aaa aag tac tga1503Ile Lys Lys Tyr500<210>12<211>500<212>PRT<213>梯牧草(Phleum pratense)<400>12Tyr Phe Pro Pro Pro Ala Ala Lys Glu Asp Phe Leu Gly Cys Leu Val1 5 10 15Lys Glu Ile Pro Pro Arg Leu Leu Tyr Ala Lys Ser Ser Pro Ala Tyr20 25 30Pro Ser Val Leu Gly Gln Thr Ile Arg Asn Ser Arg Trp Ser Ser Pro35 40 45Asp Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr Asn Val Ser His50 55 60Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser Val Arg Ile Arg65 70 75 80Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Arg Ser LeuGln Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys Met Arg Ala Val100 105 110Trp Val Asp Gly Lys Ala Arg Thr Ala Trp Val Asp Ser Gly Ala Gln115 120 125Leu Gly Glu Leu Tyr Tyr Ala Ile Tyr Lys Ala Ser Pro Thr Leu Ala130 135 140Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly Gly Asn Phe Ala145 150 155 160
Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly Ile Ala Ala Glu165 170 175Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn Gly Lys Leu His Asp180 185 190Lys Lys Ser Met Gly Asp Asp His Phe Trp Ala Val Arg Gly Gly Gly195 200 205Gly Glu Ser Phe Gly Ile Val Val Ala Trp Gln Val Lys Leu Leu Pro210 215 220Val Pro Pro Thr Val Thr Ile Phe Lys Ile Ser Lys Thr Val Ser Glu225 230 235 240Gly Ala Val Asp Ile Ile Asn Lys Trp Gln Val Val Ala Pro Gln Leu245 250 255Pro Ala Asp Leu Met Ile Arg Ile Ile Ala Gln Gly Pro Lys Ala Thr260 265 270Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr Leu Thr Pro Leu MetSer Ser Lys Phe Pro Glu Leu Gly Met Asn Pro Ser His Cys Asn Glu290 295 300Met Ser Trp Ile Gln Ser Ile Pro Phe Val His Leu Gly His Arg Asp305 310 315 320Ala Leu Glu Asp Asp Leu Leu Asn Arg Asn Asn Ser Phe Lys Pro PheAla Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro Lys Thr Val340 345 350Trp Glu Gln Ile Leu Asn Thr Trp Leu Val Lys Pro Gly Ala Gly Ile355 360 365Met Ile Phe Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro Glu Ser370 375 380Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn Ile Gln Tyr385 390 395 400Val Asn Tyr Trp Phe Ala Pro Gly Ala Ala Ala Ala Pro Leu Ser Trp405 410 415
Ser Lys Asp Ile Tyr Asn Tyr Met Glu Pro Tyr Val Ser Lys Asn Pro420 425 430Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg Asn Glu435 440 445Val Val Asn Asp Val Ser Thr Tyr Ala Ser Gly Lys Val Trp Gly Gln450 455 460Lys Tyr Phe Lys Gly Asn Phe Glu Arg Leu Ala Ile Thr Lys Gly Lys465 470 475 480Val Asp Pro Thr Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro Pro Leu485 490 495Ile Lys Lys Tyr500<210>13<211>12<212>PRT<213>鸭茅(Dactylus glomerata)<400>13Asp Ile Tyr Asn Tyr Met Glu Pro Tyr Val Ser Lys1 5 10<210>14<211>11<212>PRT<213>鸭茅(Dactylus glomerata)<400>14Val Asp Pro Thr Asp Tyr Phe Gly Asn Glu Gln1 5 10<210>15<211>17
<212>PRT<213>鸭茅(Dactylus glomerata)<400>15Ala Arg Thr Ala Trp Val Asp Ser Gly Ala Gln Leu Gly Glu Leu Ser1 5 10 15Tyr<210>16<211>15<212>PRT<213>鸭茅(Dactylus glomerata)<400>16Gly Val Leu Phe Asn Ile Gln Tyr Val Asn Tyr Trp Phe Ala Pro1 5 10 15<210>17<211>11<212>PRT<213>狗牙根(Cynodon dactylon)<400>17Lys Thr Val Lys Pro Leu Tyr Ile Ile Thr Pro1 5 10<210>18<211>22<212>PRT<213>狗牙根(Cynodon dactylon)<400>18Lys Gln Val Glu Arg Asp Phe Leu Thr Ser Leu Thr Lys Asp Ile Pro1 5 10 15
Gln Leu Tyr Leu Lys Ser20<210>19<211>16<212>PRT<213>狗牙根(Cynodon dactylon)<400>19Thr Val Lys Pro Leu Tyr Ile Ile Thr Pro Ile Thr Ala Ala Met Ile1 5 10 15<210>20<211>24<212>PRT<213>狗牙根(Cynodon dactylon)<400>20Leu Arg Lys Tyr Gly Thr Ala Ala Asp Asn Val Ile Asp Ala Lys Val1 5 10 15Val Asp Ala Gln Gly Arg Leu Leu20<210>21<211>14<212>PRT<213>狗牙根(Cynodon dactylon)<400>21Lys Trp Gln Thr Val Ala Pro Ala Leu Pro Asp Pro Asn Met1 5 10<210>22<211>15<212>PRT
<213>狗牙根(Cynodon dactylon)<400>22Val Thr Trp Ile Glu Ser Val Pro Tyr Ile Pro Met Gly Asp Lys1 5 10 15<210>23<211>19<212>PRT<213>狗牙根(Cynodon dactylon)<220>
<221>MISC_FEATURE<222>(8)..(8)<223>未确定氨基酸<400>23Gly Thr Val Arg Asp Leu Leu Xaa Arg Thr Ser Asn Ile Lys Ala Phe1 5 10 15Gly Lys Tyr<210>24<211>23<212>PRT<213>狗牙根(Cynodon dactylon)<400>24Thr Ser Asn Ile Lys Ala Phe Gly Lys Tyr Lys Ser Asp Tyr Val Leu1 5 10 15Glu Pro Ile Pro Lys Lys Ser20<210>25
<211>13<212>PRT<213>狗牙根(Cynodon dactylon)<400>25Tyr Arg Asp Leu Asp Leu Gly Val Asn Gln Val Val Gly1 5 10<210>26<211>15<212>PRT<213>狗牙根(Cynodon dactylon)<400>26Ser Ala Thr Pro Pro Thr His Arg Ser Gly Val Leu Phe Asn Ile1 5 10 15<210>27<211>36<212>PRT<213>狗牙根(Cynodon dactylon)<400>27Ala Ala Ala Ala Leu Pro Thr Gln Val Thr Arg Asp Ile Tyr Ala Phe1 5 10 15Met Thr Pro Tyr Val Ser Lys Asn Pro Arg Gln Ala Tyr Val Asn Tyr20 25 30Arg Asp Leu Asp35<210>28<211>14<212>PRT<213>黑麦草(Lolinm perenne)
<400>28Phe Leu Glu Pro Val Leu Gly Leu Ile Phe Pro Ala Gly Val1 5 10<210>29<211>9<212>PRT<213>黑麦草(Lolium perenne)<400>29Gly Leu Ile Glu Phe Pro Ala Gly Val1 5<210>30<211>22<212>DNA<213>黑麦(Secale cereale)<400>30ggctcccggg gcgaaccagt ag22<210>31<211>23<212>DNA<213>黑麦(Secale cereale)<400>31accaacgcct cccacatcca gtc 23<210>32<211>49<212>DNA<213>黑麦(Secale cereale)<400>32
gataagcttc tcgagtgatt agtacttttt gatcagcggc gggatgctc49<210>33<211>23<212>DNA<213>黑麦(Secale cereale)<400>33gctctcgatc ggctacaatg gcg23<210>34<211>25<212>DNA<213>黑麦(Secale cereale)<400>34cacgcactac aaatctccat gcaag 25<210>35<211>30<212>DNA<213>黑麦(Secale cereale)<400>35catgcttgat ccttattcta ctagttgggc 30<210>36<211>30<212>DNA<213>黑麦(Secale cereale)<400>36tacgcacgat ccttattcta ctagttgggc 30<210>37<211>30
<212>DNA<213>大麦(Hordeum vulgare)<400>37gccttgtcct gccaccacgc cgccgccacc 30<210>38<211>23<212>DNA<213>大麦(Hordeum vulgare)<400>38gctctcgatc ggctacaatg gcg23<210>39<211>25<212>DNA<213>大麦(Hordeum vulgare)<400>39cacgcactac aaatctccat gcaag 25<210>40<211>30<212>DNA<213>大麦(Hordeum vulgare)<400>40catgcttgat ccttattcta ctagttgggc 30<210>41<211>23<212>DNA<213>小麦(Triticum aestivum)<400>41
cacgcactaa atctccatgc aag 23<210>42<211>30<212>DNA<213>小麦(Triticum aestivum)<400>42tacgcacgat ccttattcta ctagttgggc30<210>43<211>23<212>DNA<213>小麦(Triticum aestivum)<400>43aagctctatc gcctacaatg gcg 23<210>44<211>25<212>DNA<213>小麦(Triticum aestivum)<400>44ggtgctcctc ttctgcgcct tgtcc 2权利要求
1.一种DNA分子，其与选自SEQ ID NO 1、3、5、7和9的序列之一的谷类花粉主要致敏原的核苷酸序列相对应。
2.一种DNA分子，其在严格条件下与权利要求1中的DNA分子杂交并来源于禾本科物种的DNA序列。
3.一种编码多肽的DNA分子，其与来自黑麦(Secale cerale)、大麦(Hordeum vulgare)或小麦(Triticum aestivum)的主要致敏原Sec c4、Hor v4或Tri a4有免疫交叉反应并来源于禾本科物种的DNA序列。
4.一种DNA分子，其与权利要求1到3中的一项或多项部分序列或部分序列的组合相对应并且编码禾本科的第4组致敏原的免疫调节、T细胞反应片段。
5.一种与权利要求1到4中的一项或多项编码免疫调节性T细胞反应片段的核苷酸序列相对应的DNA分子，其特征是通过对单个密码子的特异性突变、删除或添加而特异性修饰所述核苷酸序列。
6.权利要求5中的DNA分子，其特征是所述突变导致了相应多肽的一个、多个或所有半胱氨酸被其他氨基酸替代。
7.一种重组DNA表达载体或克隆系统，其包含功能性连接到表达控制序列的权利要求1到6中一项或多项的DNA分子。
8.一种宿主生物，其用权利要求1到6中一项或多项的DNA分子或权利要求7中的表达载体转化。
9.一种制备由权利要求1到6中一项或多项的DNA序列编码的多肽的方法，所述方法为对权利要求8中的宿主生物进行培养并从培养物中分离相应的多肽。
10.一种与SEQ ID NO 2、4、6、8和10中氨基酸序列之一对应的多肽，其由权利要求1到6中一项或多项的DNA序列编码。
11.一种与权利要求10中的氨基酸序列的成熟致敏原相应的多肽，选自以下氨基酸序列-一段SEQ ID NO 2、4或6的氨基酸序列，起始于第23位氨基酸；-一段SEQ ID NO 8或10的氨基酸序列，起始于第22位氨基酸。
12.权利要求10或11中的多肽，其用作药物。
13.一种包含至少一种权利要求12中的多肽并任选包含其他活性物质和/或佐剂的药用组合物，其用于诊断和/或治疗来自禾本科的第4组致敏原参与触发的过敏反应。
14.至少一种权利要求12中的多肽在制备用于诊断和/或治疗来自禾本科的第4组致敏原参与触发的过敏反应的药物中的用途。
15.权利要求1到6中一项或多项的DNA分子，其用作药物。
16.权利要求7中的重组表达载体，其用作药物。
17.一种包含至少一种权利要求15中的DNA分子或至少一种权利要求16中的表达载体并任选包含其他活性成分和/或佐剂的药用组合物，其用于免疫治疗性DNA疫苗接种来自禾本科的第4组致敏原参与触发的过敏反应病人和/或预防这样的过敏反应。
18.至少一种权利要求15中的DNA分子或至少一种权利要求16中的表达载体在制备用于免疫治疗性DNA疫苗接种来自禾本科的第4组致敏原参与触发的过敏反应病人和/或预防这样的过敏反应的药物中的用途。
全文摘要
本发明涉及到来自谷物类的第4组主要致敏原的DNA序列的提供，本发明也包括具有降低过敏作用的片段、部分序列的新组合及含有点突变的突变体。重组的DNA分子和其衍生而来的多肽、片段、部分序列的新组合和变异体可用于花粉过敏性疾病的治疗。由重组方法制备的蛋白可用于花粉过敏的体外和体内诊断。
文档编号A61K39/36GK1894410SQ200480037231
公开日2007年1月10日 申请日期2004年12月1日 优先权日2003年12月16日
发明者H·费比希, A·南迪, O·克洛姆维尔 申请人:默克专利股份公司