多聚酶链式反应,PCR
1)PCR多聚酶链式反应
1.Analysis of FMR-1 Gene Mutation by PCR Assay;应用多聚酶链式反应快速分析FMR-1基因突变
2.Development in Study on the Technology of PCR;多聚酶链式反应技术研究
3.Methods PCR was used to detect TSER in primary foci in 160 cases of primary gastrointestinal carcinoma and DNA sequence was an alyzed by ABI PRISM 310 gene auto-analyzer.方法 采用多聚酶链式反应 (PCR)技术检测 16 0例原发性胃肠恶性肿瘤患者 (胃癌、结直肠癌各 80例 )肿瘤原发灶TSER的表达 ,并测定其DNA序列。
英文短句/例句

1.Studies On Polymerase Chain Reaction(PCR)and Anti-HCV Antibody in Liver Diseases肝病患者丙型肝炎抗体与多聚酶链式反应检测
2.PCR Assay for Diagnosis of Streptococcus Suis Serotype 2聚合酶链式反应诊断2型猪链球菌
3.Study on Multiplex Reverse Transcription Polymerase Chain Reaction for Detection of H5,H7 and H9 Subtypes Avian Influenza Virus多重反转录聚合酶链式反应检测H5、H7和H9亚型禽流感病毒的研究
4.EXPERIMENTAL STUDY ON CALORIMETRY OF POLYMERASE CHAIN REACTION聚合酶链式反应(PCR)量热学的实验研究
5.The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
6.A MULTIPLEX PCR TO DETECT ENCARSIA FORMOSA HOST-FEEDING ON BEMISIA ARGENTIFOLII检测丽蚜小蜂取食寄主银叶粉虱的多重聚合酶链式反应
7.Diagnosis of Swine Edema Disease by the Establishment and Application of Polymerase Chain Reaction(PCR)应用聚合酶链式反应(PCR)技术诊断仔猪水肿病
8.Species identification of animal cells by polymerase chain reaction应用聚合酶链式反应鉴定实验细胞的来源种属
9.Preparation of Single-Stranded DNA Template for Pyrosequencing by Linear-after-the-Exponential-Polymerase Chain Reaction指数线性聚合酶链式反应法制备焦磷酸测序反应的单链模板
10.Determination of Rh Del phenotype by polymerase chain reaction聚合酶链式反应技术检测Rh血型D~(el)表型
11.Establishment of polymerase chain reaction(PCR) for detecting porcine parvovirus(PPV)聚合酶链式反应检测猪细小病毒方法的研究
12.Rapid Diagnosis of Fungal Keratitis Using Seminested Polymerase Chain Reaction;半巢式聚合酶链反应快速诊断真菌性角膜炎
13.Numerical Simulation of Heat Transfer for Polymerase Chain Reaction Microchip;聚合酶链式反应微芯片传热过程的数值分析
14.Diagnosis ofβ-thalassemia by single cell nested polymerase chain reaction单细胞巢式聚合酶链反应诊断β地中海贫血
15.Relief on Amplification Inhibition in PCR Added the Mixture with BSA、SSB and Nano-Ag聚合酶链式反应抑制剂的复合消除效果研究
16.Praeparation of Template DNA for Polymerase Chain Reaction一种简便快速制备多聚酶链反应模板的方法
17.Detection of Yersina pestis by Polymerase Chain Reaction with Multiplex Primers用多引物聚合酶链反应检测鼠疫耶尔森菌
18.DETECTION OF RICKETTSIA TSUTSUGAMUSHI DNA BY POLYMERASE CHAIN REACTION多聚酶链反应检测恙虫病立克次体核酸
相关短句/例句

polymerase chain reaction多聚酶链式反应
1.Total DNA was extracted from the nitrifying biofilm in strong ammonia wastewater treatment system, and two pairs of polymerase chain reaction (PCR) primers based on the 16S rDNA sequences of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were used for PCR amplification.从实验室处理高氨氮废水的生物流化床硝化系统生物膜中提取细菌总DNA,以其为模板,利用氨氧化细菌(AOB)和亚硝酸盐氧化细菌(NOB)的16S rDNA特异性引物进行多聚酶链式反应(PCR)扩增,获得AOB和NOB的特异片段。
2.Using the special primer for YRRM1,the YRRM1 gene was analyzed by polymerase chain reaction and agar gel electrophoresis method.方法 :运用多聚酶链式反应对 10例男性无精症或严重少精症的患者进行YRRM1基因位点缺失检测。
3)polymerase chain reaction(PCR)多聚酶链式反应
1.In the new proposed algorithm,all the feasible solutions of the CPP were obtained by utilizing the Polymerase Chain Reaction(PCR) techniques to eliminate false solutions from all the possible solutions,and then,the optimal solutions were extracted from those feasible solutions by utilizing surface-based techniques for DNA computation and fluorescence-labeling.新算法首先利用多聚酶链式反应技术来排除非解,从而得到中国邮递员问题的所有可行解;然后,结合基于表面的DNA计算方法与荧光标记等技术,最终从所有可行解中析出最优解。
4)RT-PCR反转录多聚酶链式反应
1.METHODS: The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.方法:采用反转录多聚酶链式反应(RT-PCR)技术,检测培养细胞中釉原蛋白mRNA的表达,采用蛋白质免疫印迹技术检测培养细胞中釉原蛋白的表达。
5)RT-PCR反转录-多聚酶链式反应
6)nest polymerase chain reaction巢式多聚酶链式反应
延伸阅读

聚合酶链式反应分子式:CAS号:性质:又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性,分解为单链。在较低温度(37~63℃)与引物退火:然后变温到72℃左右,在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链,然后又变性→退火→延伸反复进行。引物大大过量,dNTP过量,酶在高温中是较稳定的,这样经过几十个循环,目标DNA片段就按2n数递增。用此法可从mRNA中,cDNA库中扩增出目标基因。过去一般合成500~1000bp较好,现已发展出大片段(75kb)的PCR,且差错甚少。PCR技术的发展还可用于定点突变,质粒重组及FLP等方面。这一技术的作用范围日益扩大,已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破,已在分子生物学、医学、法学等领域发挥了极大的作用。