抑制差减杂交,suppression subtractive hybridization英语短句,例句大全

抑制差减杂交,suppression subtractive hybridization
1)suppression subtractive hybridization抑制差减杂交
1.In order to study the genetic differences between epidemic and nonepidemic strains of Vibrio choleraeO1 E1 Tor, the suppression subtractive hybridization was used to screen for the different genes between epidemic and nonepidemic strains of V.为了克隆与分析霍乱弧菌O1E1Tor流行株与非流行株两类菌株基因组差异片段 ,采用抑制差减杂交技术 (suppressionsubtractivehybridization ,SSH)分别以国内保留的流行株Wujiang 2及非流行株Js 32一株作为被检菌 ,另一株作为参考菌进行基因组差异研究。
2.To research salt tolerance mechanism and get the salt tolerance relative genes of Eustoma grandiflorum, a cDNA library was made by suppression subtractive hybridization technique.为了研究草原龙胆抗盐性机理,初步获得其抗盐性相关基因,分别以400mmol·L-1的NaCl及清水处理的草原龙胆叶片mRNA为tester和driver方,利用抑制差减杂交技术构建了cDNA文库。
3.Suppression subtractive hybridization (SSH) was carried out by using resistant pool as tester and susceptible pool as driver and SSH DNA library was constructed.以YW642/中8601的F2代纯合的抗病和感病单株各10株提取DNA构建成近等基因池,以抗病池为试验方,以感病池为驱动方,进行抑制差减杂交(SSH),构建差减文库。

英文短句/例句

1.Principle and manipulation result analysis for suppression subtractive hybridization(SSH);抑制差减杂交技术原理及常见操作结果分析
2.Isolation of Resistance Related Genes to Rice Sheath Blight by Suppression Subtractive Hybridization抑制差减杂交分离水稻纹枯病抗性相关基因
3.Gene Expression Profiling of Powdery Mildew Resistance in Wheat Using Suppression Subtractive Hybridization;基于抑制差减杂交方法的小麦抗白粉病相关基因表达谱研究
4.Gene Expression Proflie Induced by Water Stress in Wheat Seedling Using Suppression Subtractive Hybridization;利用抑制差减杂交技术研究小麦幼苗水分胁迫诱导的基因表达谱
5.Cloning of Fragments Related to Resistance Genes in Wheat by cDNA Capturation and Suppression Subtractive Hybridization;利用cDNA捕捉法和抑制差减杂交方法克隆小麦抗病基因相关片段
6.Research on Radiation Carcinogenesis Related Genes with Single Cell RT-PCR and Suppressive Subtractive Hybridization;应用单细胞逆转录PCR和抑制差减杂交技术研究辐射致癌相关基因
7.Application of suppression subtractive hybridization (SSH) to clone differentially expressed genes in Nilaparvata lugens (Homoptera:Delphacidae) responding to rice resistance利用抑制差减杂交技术分离受水稻抗性调控的褐飞虱基因
8.Isolation of Specifically Expressed Genes in Brown Colored Cotton by Suppression Subtractive Hybridization利用抑制差减杂交技术分离棕色棉纤维特异表达基因
9.The Preliminary Study on Extraction of Total RNA from Rice Leaves in Suppression Subtraction Hybridization Technique水稻抑制差减杂交技术中叶片总RNA提取方法的探讨
10.Construction and EST Analysis of a SSH cDNA Library of Wheat Leaves Induced by Puccinia Striifomis;条锈菌诱导的小麦抑制差减杂交文库构建（SSH）及其表达序列标签（ESTs）研究
11.Construction of Suppression Subtractive Library between Salmonella Pullorum and Salmonella Enteritidis and Cloning and Expression of ipaJ、mobA Gene;鸡白痢沙门氏菌抑制差减杂交文库的构建及ipaJ、mobA基因的克隆与表达
12.Study on Screening of Differences between Indigenous and Foreign Pig Breeds Genomes by Suppressive Subtractive Hybridization;抑制消减杂交法筛选中外猪种基因组差异研究
13.Screening of Differentially Expressed Genes in Kaposi s Sarcoma by Suppression Subtractive Hybridization;用抑制性差减杂交筛选Kaposi肉瘤相关基因
14.Suppressive subtractive hybridization analysis from red and white color patterns of Oujiang color common carp红、白两种体色瓯江彩鲤的抑制性差减杂交
15.Study on Differential Expression Genes of Caski Cell Lines Induced by Exposing to the Space Environment by Suppression Subtractive Hybridization;利用抑制性消减杂交技术筛选太空诱变宫颈癌细胞差异表达基因
16.Cloning the Genes Differentially Expressed in Tremor Rat by Suppression Subtractive Hybridization;应用抑制性消减杂交克隆震颤大鼠差异表达基因
17.Screening Differential Profile of Genes Expressed during Compatible Interactions between Puccinia Striiformis and Wheat by Suppression Subtractive Hybridization;小麦与条锈菌亲和互作的抑制性差减杂交文库构建及ESTs分析
18.Construction of subtractive cDNA Library of apoptosis-related genes in NB4 cells treated by arsenic trioxide用抑制性差减杂交构建As_2O_3诱导的NB4细胞凋亡相关基因文库

相关短句/例句

SSH抑制差减杂交
1.Isolation of Genes with Enhanced Expressions in Rice Treated with Probenazole Using SSH Technique;用抑制差减杂交技术分离烯丙异噻唑诱导水稻特异表达的基因
2.Principle and manipulation result analysis for suppression subtractive hybridization(SSH);抑制差减杂交技术原理及常见操作结果分析
3.To understand the gene expression characteristics,which involved in drought tolerance,five cDNA libraries were constructed using suppression subtractive hybridization(SSH) method with wheat seedlings sampled at 1,6,12,24 and 48 hours of water stress,respectively.利用抑制差减杂交技术,分别构建小麦幼苗在水分胁迫1 h、6 h、12 h2、4 h和48 h条件下的cDNA文库,得到6 733条EST序列。
3)Suppression subtractive hybridization (SSH)抑制差减杂交
1.) Hook], a forward subtractive cDNA library was constructed using suppression subtractive hybridization (SSH) method, which was performed using the cDNA from the mutant Dugansha clone as the tester and the cDNA from the normal Jurong 0 clone as the driver.为了获得杉木木质化过程中特异表达的基因,本研究利用抑制差减杂交技术,以杉木突变体独干杉无性系为测试方(Tester),正常的句容0号无性系为驱动方(Driver),构建了正向差减文库,获得了618个克隆。
2.Suppression subtractive hybridization (SSH) was utilized for the isolation of cDNA fragments for Euonymus japonicus ‘Zhuzi’ differentially expressed genes, and forward suppression subtractive cDNA Library of cold-regulated gene was constructed.应用抑制差减杂交(suppressionsubtractivehybridization,SSH)方法,构建冷诱导表达的正向抑制差减cDNA文库,低温处理的幼苗为tester,常温处理为driver,通过cDNA宏阵列差异筛选cDNA文库,得到604个低温诱导或表达增强的候选克隆,对其中的84克隆进行DNA测序,去除冗余的cDNA,在GenBank中进行核酸和蛋白质同源性的比较和功能分析,共有36个单一序列,有12个cDNA未找到同源序列,可能为新基因,表明采用抑制差减杂交方法与cDNA宏阵列技术分离诱导表达的基因是可行的。
3.To investigate the expression profile of maize genes induced by submergence, a subtracted cDNA library of maize seedling roots was constructed using suppression subtractive hybridization (SSH).)幼苗根部cDNA为目标群体 ,未处理 (untreated ,UT)的玉米幼苗根部cDNA为对照群体 ,进行抑制差减杂交。
4)suppression subtractive hybridization(SSH)抑制差减杂交
1.A suppression subtractive hybridization(SSH) cDNA library was constructed with an incompatible combination between wheat(Triticum aestivum) cultivar Xingzi 9104 and Puccinia striiformis f.以成株期抗条锈小麦(Triticum aestivum)品种兴资9104为材料,依照CLONTECH公司PCR-SelectTMcDNA Subtraction Kit方法构建了小麦成株期条锈菌(Puccinia striiformis)诱导的抑制差减杂交(suppression subtractive hybridization,SSH)cDNA文库。
2.To clone the differential expressional genes between the pre-metamorphosis(17 day post hatching,DPH) and pro-metamorphosis stage(23 DPH),we used suppression subtractive hybridization(SSH) to construct a cDNA library of pro-metamorphosis flounder Paralichthys olivaceus,when the RNA from pre-metamorphosis larvae was used as the driver.为了克隆变态早期牙鲆头部差异表达的基因,采用抑制差减杂交法,以变态前的仔鱼头部表达的RNA作为驱动RNA,建立了牙鲆变态早期的差减cDNA文库,并利用相对定量RT-PCR对其进行了筛选。
5)suppression subtractive hybridization抑制性差减杂交
1.Suppression subtractive hybridization(SSHs) was performed to distinguish the differentially expressed genes.取培养48h的2组细胞提取总RNA后分离mRNA,应用抑制性差减杂交法分离差异表达基因,并连接于pGEM TEasyVector,转化大肠杆菌JM109,构成差减cDNA文库,经蓝白斑筛选后挑选白色菌落,提取质粒,序列测定后进行同源性比较分析。
2.A systematic study was initiated to identify differentially expressed genes in the process of sex reversal of the grouper by using suppression subtractive hybridization (SSH) technique.应用抑制性差减杂交 (SSH)技术构建了石斑鱼性反转前后性腺组织的SMARTcDNA文库及其cDNA差减文库 ,从中随机挑取 12 0 0个克隆进行了PCR和斑点杂交筛选 ,得到 12 0个差异表达cDNA片段。
3.Objective: To Construct a subtracted cDNA library of hyperlipidemia sensitive rabbits′liver by suppression subtractive hybridization(SSH).结论:用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。
6)suppression subtractive hybridization(SSH)抑制差减杂交(SSH)