短发夹RNA,Short hairpin RNA
1)Short hairpin RNA短发夹RNA
1.Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定
2.The effect of short hairpin RNA on hTERT expression;短发夹RNA对人端粒酶催化亚基表达的影响
3.Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。
英文短句/例句

1.Construction of the shRNA expression vectors targeting survivinSurvivin靶向的短发夹RNA表达载体的构建
2.The Studies on Depressant Effect of Short-Hairpin RNA of Bcl-xL Gene Expression in HepG-2 Cells;短发夹RNA抑制人肝癌HepG-2细胞Bcl-xL基因表达的探讨
3.The Studies on Interference Effect of Short Hairpin RNA on Expression of TGF-β1 Gene in Human Lung Fibroblast;短发夹RNA抑制人肺成纤维细胞TGF-β1基因表达的研究
4.RNA interfering effects of CCL20 shRNA expressing vectors on human embryonic kidney cellsCCL20基因短发夹RNA表达载体转染人胚胎肾细胞的干扰效应
5.Inhibitory effect of β-catenin short hairpin RNA on the growth of human colon cancer xenografts in nude mice靶向β-catenin短发夹RNA抑制人结肠癌裸鼠移植瘤的实验研究
6.Silence Effect of Short Hairpin RNA on Expression of PIM-1 in Prostate Cancer Cell Lines短发夹RNA对前列腺癌细胞中PIM-1基因沉默效应的研究
7.Construction of LRIG2 Specific Short-hairpin RNA Expressing Vector and Screening of Stably Transfected Cell CloneLRIG2基因短发夹RNA表达载体的构建、鉴定和稳定株的筛选
8.Inhibitory effect of short hairpin RNA targeting IRAK-M gene for endotoxin tolerance in RAW264.7 cellsIRAK-M短发夹RNA对RAW264.7细胞内毒素耐受性的抑制作用
9.RNA interference targeting hypoxia inducible factor-1α in BGC-823 cells inhibits cell proliferation and induces cell apoptosis短发夹RNA干扰胃癌低氧诱导因子-1α表达及其临床意义
10.Gene Silencing by Systemic Delivery of Adenovirus Vector-mediated Short Hairpin RNA of Rat Angiotensin Receptor Subtype 1 in Spontaneously Hypertensive Rats;腺病毒介导的大鼠血管紧张素Ⅱ1型受体短发夹RNA在SHR的基因沉默作用研究
11.Effect of Suppressing COX-2 Expression by shRNA on Adhesion and Invasion of Human Hepatocellular Carcinoma Cell;短发夹状RNA抑制COX-2表达对人肝癌细胞株黏附侵袭力的影响
12.The Research about Interference of Nucleostemin-Specific Short Hairpin RNA in Vitro and in Vivo;核干细胞因子特异性短发夹状RNA的体内外干扰作用研究
13.Construction and Identification of Multidrug Resistance Gene MDR1 Short Hairpin Expression Plasmids;多药耐药基因MDR1短发夹状RNA表达质粒的构建与鉴定
14.Mechanism of Short Hairpin RNA Silence NF-κB Promotion Apoptosis of Hepatic Stellate Cell;应用短发夹状RNA沉默NF-κB基因促进肝星状细胞凋亡机制研究
15.The Effect of Short Hairpin RNA of Nuclear Factor Kappa B on the Apoptosis of Hepatic Stellate Cell;核因子-κB亚单位p65短发夹状干扰RNA对肝星状细胞凋亡的影响
16.Inhibition of Bcl-2 by shRNA to Induce Apoptosis in Human Lens Epithelial Cells短发夹状RNA干扰Bcl-2诱导晶状体上皮细胞凋亡的实验研究
17.Construction and identification of lentiviral vector-mediated short hairpin RNA of human EGFL7 gene人EGFL7基因短发夹结构RNA慢病毒载体的构建及鉴定
18.Reversal of Leukemia Multidrug Resistance by Sequence-Specific Short Hairpin RNA序列特异性短发夹状RNA逆转白血病多药耐药性研究
相关短句/例句

shRNA短发夹RNA
1.Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响
2.Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。
3.The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。
3)short hairpin RNA(shRNA)短发夹RNA
1.Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。
4)small hairpin RNA短发夹RNA
1.Objective:To construct FXYD6-targeted small hairpin RNA(shRNA) expression vector with pCGM30 plasmid and evaluate the inhibition effect of FXYD6 shRNA on the proliferation and protein level of FXYD6 in pancreatic cancer cells sw1990 in vitro.目的:构建FXYD6短发夹RNA(shRNA)表达载体,体外评价其对胰腺癌细胞sw1990的增殖与FXYD6蛋白表达的抑制效果。
5)Short-hairpin RNA短发夹RNA
1.Objective: To construct short-hairpin RNA expressing vectors aimed at nuclear receptor-related factor 1 (Nurrl) and study the function of Nurrl with RNA interference technology.目的:构建针对小鼠核受体相关因子1(Nurrl)的短发夹RNA(shRNA)表达载体,为进一步体内外研究Nurrl基因的功能奠定基础。
2.Objective To construct effective short-hairpin RNA (shRNA) expression vector encoding shRNA targeting LRIG2 gene, and to screen the stably transfected cell clone.目的构建针对富含亮氨酸重复序列免疫球蛋白样蛋白2(LRIG2)基因的短发夹RNA(shRNA)表达载体,建立稳定转染的细胞株,观察其对目的基因表达的影响。
6)hPARP1短发夹-RNA
延伸阅读

感染性RNA病原RNA分子式:CAS号:性质:又称感染性RNA病原RNA;壳病毒,是一种和病毒(virus)相似的感染性颗粒。为无蛋白外壳的单链RNA,分子量1.1×105~1.3×105。它是比已知病毒都小的能在宿主细胞内自主复制的病原体之一。已知的近20种类病毒中,大部分已测得了一级结构,都是无蛋白外壳的共价闭合的单链环状RNA分子。在天然状态下类病毒RNA以高度碱基配对的棒状结构形式存在。最先是由T. O. Diener等人(1969)在马铃薯纤块茎病(potato spindle tuber disease)的病株上首先发现的,在电镜下可见到这RNA分子呈50nm长的杆状分子,共有359个碱基对,并证实是游离的RNA,为此正式命名为类病毒。它通常在宿主细胞核内,借助汁液传染,分子量75000~130000,比最小病毒还小80倍。后又相继在菊花矮缩病(chrysanthemum stunt)、菊花绿斑病(chrysanthenum chlorotic mottle)、柑橘剥皮病(citrus excortis)等患病植株中分离到低分子量的病原RNA。推测它也可能存在于其他植物、动物甚至人体内。绝大部分类病毒均具有共同的结构特征:(1)位于棒状结构中心有一个高度保守的序列;(2)靠近这一保守中心区的左侧有一个多聚嘌呤区;(3)棒状结构左侧序列保守性强,右侧变异性大。它可能是通过核苷酸序列或结构改变直接与寄主细胞相互作用、干扰细胞的代谢而致病。对类病毒的研究可能为揭示生命起源和进化、生命过程的实现等生命科学的重大理论问题作出贡献。