实时定量RT-PCR,real-time quantitative RT-PCR
1)real-time quantitative RT-PCR实时定量RT-PCR
1.The clinical value of real-time quantitative RT-PCR detection for fusion genes in leukemia;实时定量RT-PCR技术检测白血病融合基因的临床应用价值
2.Development and application of Taqman real-time quantitative RT-PCR assay for detection of taura syndrome virus in shrimps;对虾Taura综合症病毒Taqman实时定量RT-PCR检测方法的建立与应用
3.And expressions of TLR9 mRNA on peripheral blood,were detected by using real-time quantitative RT-PCR method.选取HSV阳性样品50例,选取HSV阴性10例做为对照组;采用实时定量RT-PCR方法进行TLR9 mRNA检测。
英文短句/例句

1.Research and Development of Real-time Quantitative RT-PCR for Measurement of Bcr-abl mRNA Transcripts;实时定量RT-PCR检测bcr-abl mRNA的研究
2.Detection of PML-RARa Transcripts in Acute Promyelocytic Leukemia by Real-time Quantitative Reverse Transcriptase Polymerase Chain Reaction;实时定量RT-PCR检测急性早幼粒细胞白血病PML-RARa融合基因
3.Monitoring bcr-abl mRNA Levels in Chronic Myeloid Leukemia Patients by Real-time Quantitative RT-PCR;实时定量RT-PCR检测慢性粒细胞白血病患者bcr-abl mRNA水平
4.Studies on SYBR Green I Real-time Fluorescence Quantitative RT-PCR for Detecting Norovirus in Shellfish贝类中诺如病毒SYBR Green I实时定量RT-PCR检测方法研究
5.Quantitative real-time RT-PCR analysis of nestin expression in human adult bone marrow-derived neural stem cells成人骨髓源性神经干细胞中nestin表达的实时定量RT-PCR检测
6.Investigation of Expression of CD109, RFP and BRDT Genes Using Real-Time Fluorescence Quantitative RT-PCR;实时荧光定量RT-PCR检测CD109、RFP和BRDT基因表达
7.The mRNA expression of Musashi-1 gene in clonic cancer by quantitative real-time fluorescence RT-PCR实时荧光定量RT-PCR检测结肠癌中Musashi-1 mRNA的表达
8.Application of real-time fluorescent RT-PCR for quantitative detection of SARS-Coronavirus实时荧光RT-PCR在SARS病毒定量检测中的应用
9.Expression of CK19-mRNA in Peripheral Blood of Lung Cancer Patients by Real-time Fluorescent Quantitative RT-PCR and Its Clinical Significance;实时荧光定量RT-PCR检测肺癌外周血中CK19mRNA的表达及临床意义
10.Detection of iASPP mRNA in Hepatocellular Carcinoma and Hepatoma Cell Lines by Fluorescence Quantitative RT-PCR.;实时荧光定量RT-PCR检测肝癌及人肝癌细胞株iASPP mRNA
11.Development and Application of a One-step Real-time TaqMan RT-PCR Assay to Detect Duck Hepatitis Virus Type 1实时荧光定量RT-PCR检测1型鸭病毒性肝炎方法的建立和应用
12.Detection and Clinical Significance of hPMS2 mRNA in Gastric Cancer Using Real-time Fluorescent Quantitative RT-PCR实时荧光定量RT-PCR对胃癌组织中hPMS2 mRNA的检测及其临床意义
13.Real-time Quantification of MiRNAs by RNA-tailing and Primer-extension RT-PCR in Gastric CarcinomaRNA加尾和引物延伸RT-PCR法实时定量检测胃癌中miRNAs的表达
14.Establishment of a real-time fluorescent quantitative RT-PCR assay for detection of chicken IL-1β,IL-2 and IL-6 genes鸡IL-1β和IL-2及IL-6基因实时荧光定量RT-PCR检测方法的建立
15.Detection of FOXP3 mRNA expression in human peripheral blood mononuclear cells by real-time fluorescent quantitative RT-PCR实时荧光定量RT-PCR检测人外周血单个核细胞中FOXP3 mRNA的表达
16.Development of Real- Time Quantitative RT- PCR for the Detection of Dengue Virus登革热病毒RNA实时荧光定量RT-PCR检测方法的建立
17.Development of a Real-Time TaqMan RT-PCR Assay for the Detection of Foot-and-Mouth Disease Virus口蹄疫病毒实时TaqMan荧光定量RT-PCR检测方法的建立
18.Establishment of respiratory syncytial virus detection by real-time fluorescent RT-PCR呼吸道合胞病毒实时荧光RT-PCR定量检测方法的建立
相关短句/例句

real-time RT-PCR实时定量RT-PCR
1.The viruses from concentrated samples were detected by real-time RT-PCR.向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。
2.By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last.通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。
3.The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique.利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。
3)real time quantitative RT-PCR实时定量RT-PCR
1.This paper introduced the principle, method and application of Real Time Quantitative RT-PCR which includes SYBR Green I, AmpliSensor, Lightcycle and Complex technologies, In Situ PCR and Degenerate PCR.介绍了实时定量RT-PCR的原理、方法和应用,包括SYBR Green I、AmpliSensor、Lightcycle技术和Complex探针技术;同时还对原位PCR技术的原理、方法和应用,简并PCR的原理、方法和应用作了综述。
2.To investigate the feasibility of using exogenous salicylic acid(SA) to regulate the allelopathic weed suppression of rice,this paper studied the effects of different concentrations exogenous SA on the weed-suppression and physiological-biochemical characteristics of allelopathic rice PI312777,and the relative expression quantity of gene ZB8 in the rice by real time quantitative RT-PCR(FQ-PCR).为了探讨外源水杨酸(SA)调控水稻化感抑草效应的可行性,研究了不同浓度的外源SA对强化感水稻PI312777抑草效应的影响及其生理生化特性,并运用实时定量RT-PCR(FQ-PCR)技术检测SA介导的关键酶苯丙氨酸解氨酶基因(ZB8)的相对表达量。
4)Real time RT-PCR实时定量RT-PCR
1.The HOXB4 expression at mRNA level was assayed by using real time RT-PCR.本研究采用实时定量RT-PCR的方法在mRNA水平上检测HOXB4基因的表达,以观察体外扩增脐血CD34+细胞自我更新的水平。
2.To investigate the mechanism of action of anti-inflammatory and immunological regulation,the change of interleukin-2 gene in the level of mRNA in the spleen of healthy and immunosuppressive mouse was detected with the technology of real time RT-PCR.采用实时定量RT-PCR技术检测正常小鼠和免疫抑制模型小鼠脾脏内白介素-2(IL-2)基因mRNA水平表达量的变化。
5)realtime PCR/RT-PCR实时定量PCR/RT-PCR
6)Quantitative real-time reverse transcriptase-poly merase chain reaction定量实时RT-PCR
延伸阅读

immune PCR分子式:CAS号:性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。