1)recombinant PCR重组PCR
1.Construction of chicken ScFv by recombinant PCR in vitro;应用体外重组PCR技术构建鸡单链抗体基因的研究
2.Construction of Ppdc-ldh funsion gene by recombinant PCR in vitro应用体外重组PCR技术构建Ppdc-ldh融合基因
3.In this study, the gene s transmembrane domain was deleted by recombinant PCR, then the recombinant DNA was inserted into pGEX-6p-1 vector and transformed into BL21(DE3).实验尝试用重组PCR消除跨膜区,连接入pGEX-6p-1载体,构建重组表达载体,用IPTG诱导表达,SDS-PAGE鉴定结果发现,在58kD处检测到蛋白,与预期目的蛋白位置相符。
英文短句/例句
1.Construction of Ppdc-ldh funsion gene by recombinant PCR in vitro应用体外重组PCR技术构建Ppdc-ldh融合基因
2.Constructing RNAi Structare in Plant Gene by Recombiant PCR应用重组PCR技术构建植物基因RNA干扰的结构
3.Construction of GFP Gene Expression Vector Suitable for Fungal ATMT Transduction by Recombinant PCR Technology利用重组PCR技术构建适合真菌ATMT转化的GFP基因表达载体
4.Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification碱裂解法提取重组质粒DNA及PCR验证
5.The Detection of HBsAg Encoding Plasmid Copy Number in Recombinant Yeast by Real-time PCR;实时PCR法检测重组酵母中编码HBsAg质粒的拷贝数
6.Studies on the Identification of Recombinant Virus vFV282 with Nucleic Acid Probe and PCR Methods;核酸探针与PCR方法对重组病毒vFV282株的鉴定研究
7.Preparation methods' comparison of recombinant Pichia pastoris Genomic DNA for PCR毕赤酵母重组子PCR模板制备方法的比较
8.Determination of residual host genomic DNA in recombinant products by real-time PCR实时定量PCR检测重组技术产品中宿主基因组DNA残留
9.Optimization of PCR Conditions for Immunoglobulin Gene Rearrangement in Paraffin-Embedded Tissues of Non-Hodgkin's Lymphoma;NHL石蜡包埋组织免疫球蛋白基因重排PCR检测条件的优化
10.A Rapid Method by Type-specific Multiplex PCR for Clostridium perfringens on Surface of Traumatic Tissues型特异性多重PCR快速检测创伤组织中的产气荚膜梭菌
11.A Novel Method for the Determination of Recombinant Lentiviral Titer and Infectivity by qRT-PCR应用荧光实时定量PCR方法检测重组慢病毒滴度及其感染效率
12.Estimation of the HBsAg Gene Copy Number of Recombinant Hansenula Polymorpha Yeast by Fluorescence Quantitative PCR荧光定量PCR法检测重组汉逊酵母中HBsAg基因的拷贝数
13.Establishment of Standard Curves for Detection of Porcine Th1/Th2 Cytokines Genes Recombinant Plasmids Using Real-time PCR猪Th1/Th2型细胞因子重组质粒实时荧光定量PCR标准曲线的建立
14.Establishment of standard curves for detection of porcine antiviral protein genes recombinant plasmids using Real-time PCR猪抗病毒蛋白基因重组质粒实时荧光定量PCR标准曲线的建立
15.Development of a multiplex PCR/RT-PCR assay for detection of porcine reproductive and respiratory syndrome virus,porcine parvovirus,pseudorabies virus and porcine circovirus type 2多重PCR/RT-PCR技术检测PRRSV、PPV、PRV和PCV-2
16.Diagnosis Establishment Formulation of RT-PCR、Fluorescence Quantitative PCR and Mutiplex RT-PCR Detection for Japanese Encephalitis Virus;猪乙脑病毒RT-PCR、荧光定量PCR及多重RT-PCR诊断方法建立
17.Establishment of RT-PCR Assay for the Detection of Vesicular Stomatitis Virus Indiana Type and Expression of Nucleoprotein Recombinant Antigen;印第安纳型水泡性口炎病毒RT-PCR检测方法的建立及核衣壳蛋白重组抗原的表达
18.Rapid Detection of Avian Influenza Virus by Diplex RT-PCR and Real-Time RT-PCR;禽流感病毒双重RT-PCR及荧光RT-PCR快速检测
相关短句/例句
Recombinant PCRPCR重组
1.Construction of a Expression and Secretion Vector in Escherichia coli by the Recombinant PCR;应用PCR重组技术构建大肠杆菌分泌型表达载体
3)PCR mediated recombinationPCR介导重组
4)Recombinant PCR technology重组PCR技术
5)multiplex PCR多重PCR
1.Detection of Salmonella spp.,Escherichia coli and Staphylococcus aureus by Multiplex PCR;沙门菌、大肠杆菌和金黄色葡萄球菌的多重PCR检测
2.Simultaneous Detection of Common Food-borne Bacterial Pathogens in Innocuous Poultry and Seafood by Multiplex PCR Assay;多重PCR检测无公害畜禽肉和水产品中4种致病菌
3.The clinical application of multiplex PCR in diagonsis of deafness with mtDNA mutation;多重PCR诊断线粒体突变聋病的临床应用价值
6)Multiplex polymerase chain reaction多重PCR
1.Multiplex polymerase chain reaction assay for sex determination from a single cell.;应用单细胞多重PCR进行性别鉴定
2.Objective To develop a novel multiplex polymerase chain reaction (PCR) to detect multidrug-resistant Acinetobacter baumannii.目的建立一种适于临床微生物实验室快速检测多耐药鲍曼不动杆菌基因的多重PCR方法。
3.A multiplex polymerase chain reaction(PCR)-based method was designed for simultaneous detection of four main viruses involved in respiratory and/or reproductive failure in piglets(porcine circovirus type 2 ,porcine parvovirus ,porcine reproductive and respiratory syndrome virus ,and classical swine fever virus ).本试验建立一种可同时检测猪圆环病毒2型(PCV-2),猪细小病毒(PPV),猪繁殖与呼吸综合征病毒(PRRSV),猪瘟病毒(CSFV)4种病毒的多重PCR方法。
延伸阅读
诺德人体生长激素,基因重组人生长激素,健高买,重组人生长激素药物名称：重组人生长激素英文名：Somatropin别名：诺德人体生长激素,基因重组人生长激素,健高买,重组人生长激素外文名：Saizen, Norditropin, Genotropin, Norditropin, DNA-rhGH药理作用：为DNA重组技术生产的人体生长激素,是促进生长的机制之一,能刺激IGF-1和蛋白质合成,促进骨骼和体细胞的生长,利于脂肪分解和蛋白质合成,使肌肉增加,脂肪减少.适应症：用于内源性生长激素分泌不足或先天性性腺发育不全(特纳综合征)所引起的生长发育障碍,青春期前慢性肾功能不全所引起的生长发育障碍,成人生长激素不足的替代疗法(用于心功能不全等).注意点：1.配制药液时不可振荡,以免变性.2.每周剂量分7天皮下注射，注射部位应更换，如有一天漏注，则第二天不必倍量补注。3.糖尿病、妊娠、乳母慎用。4.恶性肿瘤术后，对本品过敏者，各种活动性恶性肿瘤禁用。5.不良反应可能会有甲状腺功能减退，液体潴留、伴周围水肿，曾有少数良性颅内高压病例的报告。用量用法：成人：生长激素不足：0.125iu/(kg.周） 。 先天性子宫发育不全： 1iu/(kg.周），以上剂量均分作7天皮下注射。规格：针剂：4iu, 10iu, 12iu, 16iu.类别：生殖系统药/促进子宫成熟药
