1)Two-hybrid system techniques双杂交系统技术
2)two-hybrid techniques双杂交技术
英文短句/例句
1.To Fish the Human UROC-28 Binding Protein by the Yeast Two Hybrid System;酵母双杂交技术钓取人UROC28结合蛋白
2.Screening for Proteins Interacting with AtBAG5 by Yeast Two-hybrid System;酵母双杂交技术筛选AtBAG5的下游蛋白
3.Progress in the study of HIV-1 based on two-hybridization techniques双杂交技术在HIV-1研究中的应用进展
4.Screening of the Proteins Interacting with Porcine Calsarcin-2 by Yeast Two-Hybrid Techniques应用酵母双杂交技术筛选猪Calsarcin-2蛋白结合蛋白
5.Screening of Axin-interacting Proteins by Yeast Two-hybrid System酵母双杂交技术筛选与Axin相互作用的蛋白质
6.Screening of proteins interacting with retinoic-acid-inducible gene-Ⅰ by yeast two-hybrid technique酵母双杂交技术筛选与RIG-Ⅰ相互作用蛋白
7.Screening of proteins interacting with the cytoplasmic tail of NECL1 by yeast dual-hybrid system酵母双杂交技术筛选NECL1胞内区的结合蛋白
8.Screening of Proteins Binding Gene2 Tranactivated by Hepatitis C Virus NS3 Protein from Liver cDNA Library by Yeast Two-hybrid Technique;酵母双杂交技术筛选与NS3TP2蛋白结合的肝细胞蛋白编码基因
9.Screen of ER Interacted Proteins by Yeast Two-hybrid Assasy and Functional Analysis for the Protein Complex;酵母双杂交技术筛选与ER相互作用蛋白质及其功能研究
10.Screening of SOX4-interacting Protein by Yeast Two-hybrid Assasy and Functional Analysis of the Protein Complex;酵母双杂交技术筛选与SOX4相互作用的蛋白质及其功能研究
11.Yeast Two-hybrid Based Screen for PPARg Agonist and Antagonist;基于酵母双杂交技术的PPARg激动剂和拮抗剂的筛选
12.Screen of eIF5A Interacted Proteins by Yeast Two-hybrid Assasy and Functional Analysis for the Protein Complex;酵母双杂交技术筛选与eIF5A相互作用蛋白质及其功能研究
13.Screening of CUEDC2-interacting Proteins by Yeast Two-hybrid Assasy and Functional Analysis of the Protein Complex;酵母双杂交技术筛选与CUEDC2相互作用的蛋白质及其功能研究
14.Screening of p53 Interacted Proteins by Yeast Two-Hybrid System;酵母双杂交技术筛选与p53蛋白相互作用的蛋白质研究
15.Identification of Partner Genes Interact with AtMT-A70B by Yeast-2-Hybrid System;酵母双杂交技术鉴定与拟南芥AtMT-A70B相互作用基因的研究
16.Screening of Proteins Binding to HCV Core Protein from Human Pancreas cDNA Library by Yeast Two-hybrid;用酵母双杂交技术筛选HCV核心蛋白与人胰腺cDNA文库结合蛋白基因
17.Breed New Strains of Pleurotus Cornucopiae and Agaricus Bisporus by the Technique of Single Spore Crossbreeding;应用单孢杂交技术选育双孢蘑菇、姬菇新品种
18.Detection of chromosome X and Y by two - color fluoresence in situ hybridization in human blood and bloodstain for sex identification;用双色荧光原位杂交技术鉴定血痕性别
相关短句/例句
two-hybrid techniques双杂交技术
3)Two-hybrid system双杂交系统
1.Screen for proteins that can interact with N-acetylglucosaminyltransferase Ⅲ with yeast two-hybrid system;以酵母双杂交系统筛选与N-乙酰氨基葡萄糖基转移酶Ⅲ相互作用的蛋白质
2.The yeast two-hybrid system is a very effective molecular genetic method in studying protein-protein interaction .酵母双杂交系统是研究蛋白质间相互作用的一种非常有效的分子生物学方法,具有快速和高效的特点,但也存在一些局限性。
4)two hybrid system双杂交系统
1.First part, N acetylglucosaminyltransferase Ⅲ(GNTⅢ) was used as the bait, and then from human fetal liver cDNA library, a protein which can interacts with GNTⅢ were obtained with yeast two hybrid system.用 N -乙酰氨基葡萄糖基转移酶 (GNT )和β 1 ,4-半乳糖基转移酶 (GT )作诱铒 ,以酵母双杂交系统分别从胎肝 c DNA文库中筛选到一种能与 GNT 发生相互作用的蛋白质 ,筛选到两种能与 GNT 发生相互作用的蛋白质 。
2.The yeast two hybrid system is a powerful technique for detecting in vivo protein interaction and becoming wildly used in the function study of protein.酵母双杂交系统是一种用来在体内研究蛋白质之间相互作用的一个非常有效的手段 ,并且已经得到了广泛的应用 ,它包括双杂交、反向双杂交和三杂交系统等 。
5)twohybrid system双杂交系统
1.It has been recently reported that the yeast twohybrid system is a quickly developing genetic assay which provides a highly sensitive means to det ect proteinprotein interaction in vivo.国际上出现了一种直接于细胞内检测蛋白——蛋白交互作用且灵敏度很高的遗传学新方法,酵母双杂交系统(yeasttwo-hybridsystem),可迅速鉴别新的信息传递蛋白。
6)Yeast Two-Hybrid酵母双杂交技术
1.Factors effecting on Yeast Two-Hybrid experiments and their responding Strategies;酵母双杂交技术的影响因素及其实验策略
延伸阅读
反向酵母双杂交系统确定了蛋白之间的相互作用后, 更重要的工作是研究其功能、结构及其作用的条件调节。鉴定在相互作用的一对蛋白中的任一蛋白突变对其相互作用的抑制作用, 不仅有助于探索相互作用的结构单位, 而且可作为研究体内功能的遗传学方法。这对于多个作用分子的蛋白就更为重要。 1996年vidalix x等建立了反向酵母双杂交系统(reverse two-hybrid system), 提供了简便的鉴定阻断蛋白间相互作用的方法。反向酵母双杂交系统的关键是掺入一种表达产物对细胞生长有毒的报导基因, 用于监测蛋白间的相互作用。例如酵母ura3基因表达产物是尿嘧啶合成所必需的, 同时它又可催化5-氟乳清酸(5-foa)转化为有毒物质。vidal等构建了一酵母细胞株, 其ura3的表达由含gal4结合位点的启动子严密控制。此细胞株在缺乏尿嘧啶的培养基中培育需要gal4激活结构域(gad)和gal4 dna结合结构域(gbd)的融合蛋白的相互作用的表达。而在含5-foa的完全培养基中则受gad和gbd融合蛋白相互作用的抑制。因丝赏ü秆?-foa抗性克隆从随机突变库中鉴定阻断蛋白相互作用的突变体。反向双杂交系统可更有效地蛋白间作用的关键位点或起决定作用的个别氨基酸, 进而分析蛋白结构和功能的关系。此外此系统还可筛选能阻止某些蛋白间相互作用的肽或小分子物质用作临床治疗制剂。
