反转录聚合酶链反应,RT-PCR英语短句,例句大全

反转录聚合酶链反应,RT-PCR
1)RT-PCR反转录聚合酶链反应
1.[Methods] After rats of groups A and B were administrated by gavage with liquid of Epimedium and Fructus Ligustri Lucidi for 14 days respectively, group C as the control, RT-PCR technique was used for examining mRNA expression and immunohistochemistry was used for examining protein exp.方法SD雄性大鼠,分为A、B、C3组,A、B分别给予补肾阳中药淫羊藿和补肾阴中药女贞子持续灌胃14d,C组作为空白对照,采用反转录聚合酶链反应(RT-PCR)方法检测雌激素受体ERβmRNA表达量,免疫组化染色检测ERβ蛋白的分布及表达。
2.Total RNA were abstracted from retinal and choroidal tissue and the mRNA of PEDF was detected by RT-PCR.采用半定量反转录聚合酶链反应(RT-PCR)方法检测PEDF mRNA在大鼠视网膜中的表达水平的变化。
3.Methods HUVECs were incubated with different concentrations of LHS and the TF mRNA expression was detected by reverse transcript-polymerase chain reaction (RT-PCR).测定不同浓度LHS处理前后组织因子表达,应用反转录聚合酶链反应(RT-PCR)法检测TFmRNA水平。
2)Reverse transcriptase polymerase chain reaction反转录聚合酶链反应
1.Methods The c-myc cDNA was acquired by reverse transcriptase polymerase chain reaction(RT-PCR) after isolating total RNA from tissue of human esophageal squamous cell carcinoma(ESCC).方法用反转录聚合酶链反应(RT-PCR)法从人食管鳞癌组织的总mRNA中反转录扩增c-myc cDNA,将纯化的PCR产物与pGEM-T Easy载体进行连接,转化宿主菌DH-5α,然后挑选阳性克隆重组质粒,分别用PCR扩增、限制性核酸内切酶EcoRⅠ酶切进行鉴定并测序分析。
3)reverse transcription polymerase chain reaction反转录聚合酶链反应
1.METHODS: The expression levels of mRNA and protein of survivin were investigated respectively by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods in cell lines BT325 and SHG-44.方法 :采用反转录聚合酶链反应 (RT PCR)和免疫组化方法分别检测SurvivinmRNA及蛋白在BT32 5和SHG 4 4细胞系中的表达水平。
4)reverse transcription-polymerase chain reaction反转录聚合酶链反应
5)reverse transcriptase-polymerase chain reaction反转录聚合酶链反应
1.Methods Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank.方法在2003年秋冬季腹泻流行期间在南方医院儿科收集临床诊断为病毒性腹泻患儿的粪便标本,并采用半套式反转录聚合酶链反应(RT-PCR)方法检测。
6)RT-PCR反转录-聚合酶链反应
1.Studies on the Detection of Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR;用反转录-聚合酶链反应检测猪繁殖与呼吸综合征病毒的研究
2.The expression of CGRP mRNA in trigeminal ganglion were detected with reverse transcription-polymerasa chain reaction(RT-PCR).方法:采用反转录-聚合酶链反应(RT-PCR)技术检测正畸加力和撤力后不同时期大鼠三叉神经节CGRP mRNA水平的变化。
3.Methods The mRNA expression of the survivin gene was evaluated by reverse transcription-polymerase chain reaction(RT-PCR) in 40 laryngeal carcinomas and in 52 non-neoplastic laryngeal tissues.方法应用反转录-聚合酶链反应(RT-PCR)检测喉鳞癌组织、癌旁组织、非典型增生组织和正常对照组织中Survivin mRNA的表达水平。

英文短句/例句

1.Effects of cyclic tension force on the expression of BGLAP in human periodontal ligament cells实时定量反转录-聚合酶链反应检测机械力对人牙周膜细胞骨钙素表达的影响
2.Dection of enterovirus infection by reverse transcription and polymerase chain reaction应用逆转录聚合酶链反应检测肠道病毒感染
3.Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients逆转录-聚合酶链反应在乙型脑炎诊断中的建立
4.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
5.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究
6.Rapid Detection of Tobacco Mosaic Virus from Crude Samples on Oscillatory-flow Reverse Transcription Polymerase Chain Reaction Microfluidics微流控振荡流反转录聚合酶链式反应快速检测烟草花叶病毒
7.Study on Multiplex Reverse Transcription Polymerase Chain Reaction for Detection of H5,H7 and H9 Subtypes Avian Influenza Virus多重反转录聚合酶链式反应检测H5、H7和H9亚型禽流感病毒的研究
8.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
9.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
10.Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
11.Analysis of reserved conditions of blood sample for detection of HCV RNA with RT-PCR逆转录聚合酶链反应检测HCV RNA血标本保存条件分析
12.Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
13.Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction逆转录聚合酶链式反应快速检测水稻条叶枯病毒
14.DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
15.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
16.Development of a SYBR Green Real-time RT-PCR for Detection of Mumps Virus GeneSYBR Green荧光逆转录-聚合酶链反应检测流行性腮腺炎病毒基因
17.Reverse Transcription and cDNA Amplification of the 76-118 Strain of Hantann Virus肾综合征出血热病毒76－118株的反转录及聚合酶链反应扩增、鉴定的初步研究
18.Development of an Antigen Capture Reverse Transcriptase/Polymerase Chain Reaction for Detection Foot-and-Mouth Disease Virus;抗原捕获反转录聚合酶链式反应（AC-RT/PCR）检测口蹄疫病毒方法的建立

相关短句/例句

Reverse transcriptase polymerase chain reaction反转录聚合酶链反应
1.Methods The c-myc cDNA was acquired by reverse transcriptase polymerase chain reaction(RT-PCR) after isolating total RNA from tissue of human esophageal squamous cell carcinoma(ESCC).方法用反转录聚合酶链反应(RT-PCR)法从人食管鳞癌组织的总mRNA中反转录扩增c-myc cDNA,将纯化的PCR产物与pGEM-T Easy载体进行连接,转化宿主菌DH-5α,然后挑选阳性克隆重组质粒,分别用PCR扩增、限制性核酸内切酶EcoRⅠ酶切进行鉴定并测序分析。
3)reverse transcription polymerase chain reaction反转录聚合酶链反应
1.METHODS: The expression levels of mRNA and protein of survivin were investigated respectively by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods in cell lines BT325 and SHG-44.方法 :采用反转录聚合酶链反应 (RT PCR)和免疫组化方法分别检测SurvivinmRNA及蛋白在BT32 5和SHG 4 4细胞系中的表达水平。
4)reverse transcription-polymerase chain reaction反转录聚合酶链反应
5)reverse transcriptase-polymerase chain reaction反转录聚合酶链反应
1.Methods Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank.方法在2003年秋冬季腹泻流行期间在南方医院儿科收集临床诊断为病毒性腹泻患儿的粪便标本,并采用半套式反转录聚合酶链反应(RT-PCR)方法检测。
6)RT-PCR反转录-聚合酶链反应
1.Studies on the Detection of Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR;用反转录-聚合酶链反应检测猪繁殖与呼吸综合征病毒的研究
2.The expression of CGRP mRNA in trigeminal ganglion were detected with reverse transcription-polymerasa chain reaction(RT-PCR).方法:采用反转录-聚合酶链反应(RT-PCR)技术检测正畸加力和撤力后不同时期大鼠三叉神经节CGRP mRNA水平的变化。
3.Methods The mRNA expression of the survivin gene was evaluated by reverse transcription-polymerase chain reaction(RT-PCR) in 40 laryngeal carcinomas and in 52 non-neoplastic laryngeal tissues.方法应用反转录-聚合酶链反应(RT-PCR)检测喉鳞癌组织、癌旁组织、非典型增生组织和正常对照组织中Survivin mRNA的表达水平。