T7启动子,T7 promoter
1)T7 promoterT7启动子
1.Construction of expression system controlled by T7 promoter at different expression level in mammalian cells;在哺乳类动物细胞中呈现不同表达水平的T7启动子表达系统构建
2.Methods cDNA of TIMP-1 was obtained by RT-PCR,and coloned PGEM-T vector,and identified by PCR and sequencing;and coloned recombinant plasmid PGEM-T/TIMP-1 was used as template to obtain cDNA of TIMP-1 with T7 promoter by PCR;and dsRNA of TIMP-1 was obtained by transcription.方法 RT PCR获得TIMP 1cDNA ,克隆于PGEM- T载体上,PCR鉴定并进行序列分析;以PGEM- T/TIMP- 1重组质粒为模板,PCR获得带有T7启动子的TIMP 1cDNA ;体外转录获得TIMP- 1dsRNA。
3.Flask_shaking experiments and batch fermentations were performed to investigate the effects of medium, inducer and glucose_feeding mode on bFGF formation directed by a T7 promoter.在前期试验的基础上 ,重点探索培养基、诱导剂及葡萄糖添加方式对T7启动子指导下的bFGF合成的影响 。
2)T7 promoter systemT7启动子系统
3)T7 terminatorT7终止子
4)promoter/non-promoter启动子/非启动子
5)promoter[英][pr?'m??t?(r)][美][pr?'mot?]启动子
1.A DNA Autonomous Replication Origin with Promoter Function in Yeast;一个具有启动子功能的酵母DNA自主复制起始区
2.Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1;人内皮细胞高表达脂多糖相关因子1启动子荧光素酶报告基因重组体的构建
3.Effect of Aspirin on humanα_2(Ⅰ) collagen promoter induced by rhCD40L;rhCD40L及阿司匹林对人I型胶原α_2(I)链启动子活性表达的影响
英文短句/例句

1.promoter suppression启动子抑制,启动子阻抑[一个启动子抑制另一个启动子的转录]
2.core promoter element核心启动子元件,启动子核心元件
3.promoter occulsion启动子封阻[上游启动子对下游启动子的阻碍作用]
4.chicken ovalbumin upstream promoter鸡卵白蛋白上游启动子
5.Study on the Promoter Activity in the HCV 5 UTR Sequence;HCV 5 UTR启动子活性研究
6.Screening and Characterization of Promoters in Rice Promoter Trap Lines;利用水稻启动子捕获系筛选启动子的研究
7.Natural Compounds Induce Klotho Promoter Activity and Klotho Promoter Analysis天然化合物诱导Klotho启动子活性及Klotho启动子分析
8.The structure of the seed-specific promoter matches the characteristics of the promoter Ⅱ, having a basal promoter, initiator and upstream element.种子特异性启动子的结构符合Ⅱ类启动子的特点,具有基本启动子、起始子和上游元件。
9.Cloning and Functional Analysis of OsBTF3p, a Rice Gene Promoter Directing GUS Expression;水稻基因启动子OsBTF3p的克隆及其启动活性分析
10.Study on Promoter Sequence with Function for Transcription Initiation of BMPR-2 Gene;BMPR2基因启动子具有启动功能片段的研究
11.Molecular Cloning and Activity Analysis of a Rice Gene Promoter OsBTF3p水稻基因启动子OsBTF3p的克隆和启动活性分析
12.The Preliminary Analysis of Core Sequences at Human Integrin β1 Promoter人β1整合素启动子核心启动序列初步分析
13.promoter damping启动子减弱(作用)[邻近的或插入的启动子对增强子活性的减弱作用]
14.The Molecular Mechanism Study of LPS Induced the uPA Promoter ElementsLPS诱导uPA启动子的分子机制的研究
15.The Study on Transcription Factor Promoters of Oryza Sativa L. by ECOTILLING栽培稻转录因子启动子的ECOTILLING分析
16.Cloning and Function Analysis of ats1A Gene Promoter and ats2bB Gene Promoter from Arabidopsis Thaliana;拟南芥atslA基因启动子和ats2B基因启动子的克隆及其功能分析
17.Study on Cpcp (Pollen Coat Protein Gene) Promoter Expression of Tea Plant (Camellia Sinensis)茶树花特异表达启动子—花粉壁蛋白基因启动子表达研究
18.major late promoter主要晚期启动子[如见于腺病毒]
相关短句/例句

T7 promoter systemT7启动子系统
3)T7 terminatorT7终止子
4)promoter/non-promoter启动子/非启动子
5)promoter[英][pr?'m??t?(r)][美][pr?'mot?]启动子
1.A DNA Autonomous Replication Origin with Promoter Function in Yeast;一个具有启动子功能的酵母DNA自主复制起始区
2.Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1;人内皮细胞高表达脂多糖相关因子1启动子荧光素酶报告基因重组体的构建
3.Effect of Aspirin on humanα_2(Ⅰ) collagen promoter induced by rhCD40L;rhCD40L及阿司匹林对人I型胶原α_2(I)链启动子活性表达的影响
6)35s promoter35s启动子
1.The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.建立了玉米转基因成分中35S启动子非同源模板的竞争定量PCR检测系统。
2.However, the most generally used consititutive CaMV 35S promoter often fail in some incidences, especially for those genes in male organgenesis and male gametogenesis.然而,在拟南芥遗传学研究中最常用的花椰菜花叶病毒35S启动子在花器官中,尤其在雄蕊中往往并不能做到持续而强烈地表达目的基因,所以有利用35S启动子无法互补突变体表型,或者观察不到异位表型的事例的报导。
3.And also it is strong recommended that the biosafe construct should have no any uncertain element and exogenous DNA sequence such as antibiotics gene,35S promoter etc.本研究以pCAMBIA1300载体为基础,基因操作去除pCAMBIA1300质粒上的潮霉素抗性基因和花椰菜花叶病毒的35S启动子序列,构建了两种只含有水稻蜡质基因启动子引导蜡质基因反义片段的表达载体,p13AWY-1和p13AWY-2。
延伸阅读

启动子分子式:CAS号:性质:(一)在DNA分子上,可被RNA聚合酶识别并与之结合的一段序列,位于结构基因的上游,在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子,以期取得更多的表达量。(二)一种致癌因素,在癌变过程的第二阶段或多阶段机制中造成第二阶段(促进阶段);一种辅助致癌物。