反向遗传操作,reverse genetics
1)reverse genetics反向遗传操作
1.In this study,a recombinant VSV Indiana strain rVSV-EGFP expressing enhanced green fluorescent protein(EGFP)was generated by reverse genetics techniques.通过负链RNA病毒反向遗传操作技术,成功救获了表达增强绿色荧光蛋白(Enhanced green fluorescent protein,EGFP)的重组印地安纳株水疱性口炎病毒(Vesicular stomatitis virus Indiana serotype)、救获的重组病毒保持了野生型VSV高滴度生长特性,细胞连续传代10次仍保持GFP的稳定表达及生物学特性不变。
英文短句/例句

1.Reverse Genetics Systems of NDV: Basic and Applied Research Straine;新城疫病毒反向遗传操作基础与应用研究
2.The Development of the Reverse-genetics System and Gene-function Research for Infectious Bursal Disease Virus;鸡传染性法氏囊病病毒反向遗传操作系统的建立及基因功能研究
3.Evolution Analysis and the Establishment of Reverse Genetics System of an H5N1 Swine Influenza Virus;一株H5N1亚型猪流感病毒的进化分析及其反向遗传操作系统的建立
4.Reverse Genetic Manipulation of Coding Regions of Porcine Reproductive and Respiratory Syndrome Virus猪繁殖与呼吸综合征病毒(PRRSV)蛋白编码基因区的反向遗传操作
5.Study on the Establishment of Reverse Genetics System of Vesicular Stomatitis Virus Indiana Serotype and the Construction of Recombinant Viruses水疱性口炎病毒印第安纳株反向遗传操作系统的建立及重组病毒的构建
6.Establishment of H9N2 Subtype AIV Reverse Gentic System and the Pathgenicity to MammalH9N2亚型禽流感病毒反向遗传操作系统的建立及对哺乳动物致病性的研究
7.Analysis of Tobaccos Genetic Diversity and the Nitrogen Metabolism Genetic Operation;烟草遗传多样性分析及其氮代谢的遗传操作
8.[Undo] Reversible actions. Users should be allowed to recover from errors. Reversible actions also encourage exploratory learning.用户应该被允许反向操作,现实证明,反向操作有利于探索性的学习。
9.Reverse Genetics System of Rabies Virus CTN Strain狂犬病病毒CTN株反向遗传系统的建立
10.Methods Optimize the routes by using the genetic algorithm (GA) whose crossover operator is improved.方法利用遗传算法进行优化,对遗传算法的交叉操作进行改进。
11.Genetic Engineering for the Enhancement of Aluminum Tolerance in Medicago Sativa L.;提高紫花苜蓿(Medicago sativa L.)耐铝毒能力的遗传操作
12.Enhanced the HCHO Detoxification Capacity of Plant by Genetic Engineering;通过遗传操作提高植物代谢甲醛的能力
13.An Optimal Path Programming of the Manipulators Based on Hga;基于混合遗传算法的操作臂最优路径规划
14.Legitimacy Analysis of the Operatings of Self-adaptive Population Dimension Genetic Algorithm;种群规模自适应遗传算法操作中的合法性分析
15.Genetic Algorithm based Fuzzy Modeling of Operator Functional States基于遗传算法的操作员功能状态模糊建模
16.Improved Genetic Operator PSO and Its Application in VRP改进遗传操作PSO算法及其在VRP中的应用
17.Accelerating Convergency Genetic Algorithm of Improved Operator一种改进操作算子的加速收敛遗传算法
18.Enterprise interoperability resource selection based on parallel genetic algorithm基于并行遗传算法的企业互操作资源选择
相关短句/例句

reverse genetics反向遗传操作技术
1.High-yield H3N2 subtype swine influenza virus for large-scale vaccine production in cell culture was generated by reverse genetics.为拯救出一株能够在动物传代细胞中高水平复制的H3N2亚型猪流感疫苗株,利用反向遗传操作技术,将A/Goose/Dalian/3/01(H9N2)毒株的PB1、PA、NP、M、NS基因和A/PR/8/34毒株的PB2基因作为内部基因与猪流感病毒A/Swine/Henan/S4/01(H3N2)毒株的HA、NA基因进行重组,成功拯救出了具有高度细胞适应性毒株rH3N2株,该毒株接毒MDCK细胞60h后,血凝价可以达到1∶512,表明该毒株具有高度适应细胞繁殖特性,为H3N2亚型猪流感病毒细胞培养型疫苗的研制奠定了基础。
2.H9N2 influenza A virus is currently prevalent in China and reverse genetics has become very useful for influenza virus research.反向遗传操作技术的建立,为对禽流感病毒基因功能和疫苗构建等方面的研究提供了新的手段。
3)reverse genetics technique反向遗传操作技术
4)genetic operation遗传操作
1.Further,the proposed ant colony algorithm was succeeded in the continuous space optimization problem through dividing the space into sub-domains and adopting the genetic operation.新型蚁群算法通过将解空间划分成若干子域,并引入遗传操作,实现连续优化问题的寻优。
2.Then the encoding model and genetic operation about GA in solving TSP are discussed.文章介绍了TSP问题和遗传算法的基本原理以及特点 ;针对解决TSP问题 ,论述了遗传算法在编码表示和遗传操作算子等方面的应用情况 ,分别指出了顺序表示、路径表示和布尔矩阵表示的优缺点 ,阐述了三种基本的操作算子的应用现状 ;最后 ,简单说明了混合遗传算法在求解TSP问题中的应用并对遗传算法解决TSP问题的前景提出了展望 。
3.Float encoding genetic algorithm (FGA) is adopted for genetic algorithm, and corresponding genetic operation(selection, crossover and mutation) are designed.对多极值函数的全局优化问题 ,采用十进制浮点数对遗传算法进行编码 ,综合设计出相应的选择、交叉与变异遗传操作 ,得到浮点数编码的遗传算法 (Float encodingGeneticAlgorithm ,FGA) 。
5)genetic manipulation遗传操作
1.It is also discussed the important usage of these genes as the molecular target for increasing crop grain yields by genetic manipulation.本文主要论述作物矮化基因和分蘖相关基因的研究进展 ,以及这些基因作为遗传操作的分子靶标在增加粮食产量方面的重要应用价值。
2.Besides,the application of genetic manipulation of embryonic cells in animal production was forecasted.胚胎遗传操作是现代生物技术研究最为活跃的领域之一。
3.In the paper,various problems caused by it have been analysed based on the research of genetic manipulation,meanwhile some relevant countermeasure will be put forward.遗传操作已成为 2 1世纪生命科学的主导技术 。
6)reverse genetics反向遗传
1.Establishment of infectious bursal disease virus(IBDV) reverse genetics system using T7 RNA polymerase.;基于T7 RNA聚合酶的传染性法氏囊病病毒反向遗传系统的建立
2.Using reverse genetics, we constructed a series of single-gene or multiple-gene reassortants from these two viruses.利用反向遗传技术构建一系列单个和多个基因组合替换基因重排病毒,并验证重排病毒在麻鸭上的致病力。
3.The reverse genetics could translate the RNA into cDNA,it extremely p.RNA的结构不稳定成为阻碍RNA病毒研究的主要障碍,近年来兴起的反向遗传技术将RNA病毒的基因组转化为cDNA,从而使RNA病毒的基因操作成为可能,也使RNA病毒的研究获得了快速的发展。
延伸阅读

反向遗传学分子式:CAS号:性质:又称反向遗传学。通过在体外改变基因序列来观察体内的遗传学效应。为反向生物学的一个分支。