RACE,Rapid amplification of cDNA ends英语短句,例句大全

RACE,Rapid amplification of cDNA ends
1)Rapid amplification of cDNA endsRACE
1.The porcine CD8α full-length cDNA was amplified and cloned from total RNA of lymphocytes by RT-PCR and RACE(rapid amplification of cDNA ends).应用RT-PCR和RACE技术从猪胸腺细胞总RNA中扩增克隆了猪CD8α的全长cDNA基因。
2.By the methods of RT-PCR and rapid amplification of cDNA ends(RACE) we isolated a HSP70 gene(CsHSP70) from Capparis spinosa,a specific resistant plant in arid zone.以干旱区特有的抗逆性植物刺山柑为材料,利用RT-PCR结合cDNA末端快速扩增(rapid amplification ofcDNA ends,RACE)方法,克隆了一个HSP70基因,将该基因进行原核表达,通过对大肠杆菌进行温度胁迫实验,并计算存活率来验证该基因对细胞的保护作用。

英文短句/例句

1.Cloning of Alfalfa Phytochrome A Gene Using Rapid Amplification of cDNA Ends;RACE法克隆紫花苜蓿光敏色素A基因
2.Amplification Carbo-tip of L Gene by 3 -RACE and Sequence Analysis of Rabies Virus;RV野毒株L基因C端3’-RACE扩增及序列分析
3.Research of Adonis CBF1 by SMART RACE and Realtime PCR;RACE法获取顶冰花CBF1基因及其抗冻性质研究
4.Cloning of △～6-Fatty Acid Desaturase Gene cDNA from Oenothera Biennis Using Technology of Race;应用RACE技术克隆月见草△～6-脂肪酸脱氢酶基因cDNA
5.Application of RACE method on the construction of full-length cDNA library for floral buds in Forsythia suspenseRACE法在连翘花蕾全长cDNA文库构建中的应用
6.Amplification and cloning of angtiotension-coverting enzyme 2 gene from Rhinolophus sinicus by RACE TechniqueRACE技术扩增及克隆中华菊头蝙蝠ACE2基因
7.As its name suggests, RACE will replace the cesium so familiar to clock makers with a different alkali element.顾名思义，RACE将以另一种硷金属元素取代众所熟悉的铯。
8.The Cloning Dehydrin Gene wzy2 by Race and Analysis of Free Amino Acids in Wheat under Water-Stress;水分胁迫下小麦脱水素基因wzy2的RACE克隆及游离氨基酸的研究
9.Amplification of the MIF Gene by Using RACE Method and Identification in Early Stage Squamous Carcinoma of the Cervix;RACE法扩增早期宫颈鳞癌淋巴转移相关基因（MIF）的全长及其克隆、验证
10.Cloning and Sequencing of Two cDNAs Encoding the Catalytic Beta Subunit of Protein Phosphatase 1 and Metalloprotease of Flatfish Turbot Scophthalmus Maximus by RACE Technique;RACE技术克隆大菱鲆蛋白磷酸酶1催化亚基基因和金属蛋白酶基因及其序列分析
11.Preliminary Cloning the 3-ketoacyl-CoA Synthase (KCS) with RACE Technique and Establishment of Regeneration System in Linumusita TissimumRACE初步克隆胡麻β-酮脂酰-CoA合酶基因以及胡麻再生体系建立的研究
12.imperfect rhyme不完全韵(love, move, phase, race等)
13.Compare run, trot, jog, gallop, sprint and race.试比较run、 trot、 jog、 gallop、 sprint、 race这几个词.
14.Race suggests a need to run very fast,not always in competition .race指有快速跑的必要,但不一定总是指比赛
15.Molecular cloning and analysis of the 3′ end sequence of four strains of duck hepatitis virus by 3′ RACERace法扩增4株鸭肝炎病毒3′末端序列及其克隆分析

相关短句/例句

3 RACE3'RACE
1.3 RACE Amplification and Sequence Analysis of a Strain of Norovirus;一株诺如病毒的3’RACE扩增及基因序列分析
2.Objective:To amplify the 3 -cDNA end of phospholipase D(PLD)gene of Agropyron mongolicum Keng by 3 RACE technique and to analyze the sequence in the experiment,the 3 -cDNA end cloning establish foundation in order to obtain the cDNA sequence and research the function of PLD gene.目的:采用3’RACE方法对蒙古冰草磷脂酶D(PLD)基因3’端全序列进行了快速扩增和序列分析,为得到全序列及研究该基因的功能奠定基础。
3.The cDNA of DMrt1(DM-related transcription factor1)was amplified by 3 RACE-PCR(Rapid Amplification of cDNA Ends-Polymerase chain reaction)strategy using the total isolated RNA as template.从奥利亚罗非鱼精巢提取总RNA,采用RT和3’RACE(rapid amplification of cDNA ends)法分离和测定了奥利亚罗非鱼DMRT1(DM-related transcription factor1)cDNA的3’序列,扩增得到1086bp的片段,阅读框共编码254个氨基酸,序列分析结果表明,奥利亚罗非鱼DMRT1基因的氨基酸序列与尼罗罗非鱼DMRT1基因的同源性为99%,与黑鲷、黄鳝、新月鱼、虹鳟、青鳉鱼等动物的DMRT1cDNA和尼罗罗非鱼、奥利亚罗非鱼等动物的DMO(DM-domain gene in Ovary)cDNA的同源性分别为:80%、78%、73%、62%、41%、26%、26%。
3)3'-RACE3-'RACE
1.In this study,a cDNA fragment of dehydroascorbate reductase(DHAR) including whole 3 -end was cloned from Royal Gala by RT-(nest) PCR and 3′-RACE.以皇家嘎拉苹果叶片为材料提取总RNA,合成cDNA第一链后,先利用巢式PCR获得苹果脱氢抗坏血酸还原酶(DHAR)基因中间片段,再利用3-′RACE方法进一步获得3′末端核酸序列,共得到840 bp包含完整3′末端的DHAR基因cDNA片段,其序列与烟草、水稻、番茄中DHAR基因序列的同源性均大于70%,证明所克隆到的核苷酸片段确为苹果DHAR基因cDNA片段。
4)3-RACE3'-RACE
1.Amplification Carbo-tip of L Gene by 3 -RACE and Sequence Analysis of Rabies Virus;RV野毒株L基因C端3’-RACE扩增及序列分析
2.Methods:An objective gene was isolated by using a RT-PCR and 3 -RACE approachs and two degenerate PCR primers against conserved sequences in the MADS-box and in the C-terminal region of several plants.方法:根据多种植物的MADS-box保守区及其C-端序列设计一对简并性引物,利用RT-PCR及3’-RACE的方法分离目的基因。
5)RACE methodsRACE法
6)3 RACE or 5 RACE3 RACE或5 RACE