1)luciferase gene荧光素酶基因
1.Construction of expression plasmid vectors containing the luciferase gene of an algae Dunaliella salina;杜氏盐藻荧光素酶基因表达载体的构建
2.Highly efficient expression of luciferase gene cDNA eukaryotic expressing vector in Bacteria;萤火虫荧光素酶基因cDNA真核表达载体在细菌中的高效表达
3.The eukaryotic expressing vector pGL 2 of luciferase gene cDNA can express efficiently in prokaryotic cells.萤火虫荧光素酶基因 c DNA真核表达载体 p GL2 可在原核细胞中高效表达 。
英文短句/例句
1.Optimization of the expression of luc gene in E. coli荧光素酶基因luc在大肠杆菌中表达条件优化
2.Real time ex vivo detection and dynamic monitoring of in vivo expression of secreted luciferase gene injected by hydrodynamic method体外实时动态监测水动力注射分泌型荧光素酶基因的表达
3.The Gene Expression and Pathological Changes after Adenovirus-mediatedluciferase Gene Transfer to Rat Submandibular Glands;大鼠颌下腺腺病毒介导荧光素酶基因转导后基因表达及组织结构的研究
4.Construction of Dual-Luciferase reporter plasmid assay system for human NOX1 geneNOX1基因荧光素酶报告基因系统的建立
5.Construction and identification of luciferase reporter gene vector containing HMGB1 promoterHMGB1启动子荧光素酶报告基因的构建及鉴定
6.Viviperception of Transgenic Human Embryonic Stem Cells Labeled by Luciferase荧光素酶标记转基因人胚胎干细胞的活体观察
7.Creation and Identification of Luciferase Reporter Gene Containing Human Bax Promotor人Bax启动子荧光素酶报告基因的构建和鉴定
8.Construction and identification of human Puma promotor luciferase report gene vector人Puma启动子荧光素酶报告基因的构建和鉴定
9.Gene Imaging by Luciferase in Vitro Detecing Therapeutic Effect of hTRAIL for Lung Cancer A549 Cells;荧光素酶体外监测hTRAIL基因治疗肺癌A549细胞的基因成像研究
10.Construction of luciferase reporter gene plasmid containing hBD-2 and mutant correlated with Crohn's diseaseβ-防御素-2荧光素酶报告基因载体及克罗恩突变体的构建
11.Construction and analysis of the luciferase reporter vectors of human SMYD3 gene promoter人SMYD3基因启动子荧光素酶报告载体的构建与分析
12.The Construction of Luciferase Reporter Gene Vectors Containing Different Haplotypes DNA of Human PLUNC Gene Promoter RegionPLUNC基因启动子区荧光素酶报告载体的构建与鉴定
13.Temporal-spatial Expressing Analysis of zds Gene of Papaya by Real-Time QF-PCR番木瓜ζ-胡萝卜素脱氢酶基因时空表达的荧光定量PCR分析
14.Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
15.To Detect Gene Therapeutic Effect of hTRAIL for Lung Cancer A549 Cells Nude Mice Transplantated Tumor in Vivo by Luciferase Bioluminescence Imaging荧光素酶光成像适时监测hTRAIL基因治疗肺癌A549细胞裸鼠移植瘤的实验研究
16.Expression of the Glucoamylase Gene of Aspergillus Niger in Pseudomonas Fluorescens;黑曲霉糖化酶基因在荧光假单胞菌中的表达
17.An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin.荧光素酶存在于生物性发光组织的细胞中,催化荧光素氧化的酶
18.Improving the Lipase Production of Pseudomonas fluorescence 26-2 through Transform Its Lipase Gene into the Originally Strain导入脂肪酶基因提高荧光假单胞菌26-2的产酶效率
相关短句/例句
Firefly luciferase gene虫荧光素酶基因
3)Lux genes荧光素酶基因(Lux)
4)luciferase reporter gene荧光素酶报告基因
1.These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.方法提取人内皮细胞基因组DNA,设计引物克隆EOLA1基因上游不同长度的基因片段,插入到含荧光素酶报告基因的载体pGL3-Basic中,经限制性内切酶酶切鉴定、测序。
2.[Method] Clonging the promoters of CYP3A4 and CYP2B6 which contained the elements that hPXR, a kind of nuclear receptor, can recognize and bind, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.方法利用双荧光素酶报告基因系统,将包含hPXR蛋白识别和结合调控元件的CYP3A4和2B6启动子序列插入报告基因上游,将表达载体和报告载体共转染HepG2细胞,用含有利福平或DMSO培养基培养48h后裂解进行双荧光素酶活性检测。
3.Amplified apolipoprotein A-I(apoA-I) promoter gene(376 bp) containing essential transcription regulatory elements from human genomic DNA was fused upstream to the luciferase reporter gene and neomycin resistance gene in the constructed pGL3B-neo vector.PCR扩增含有apoA-I基因的转录调控序列的启动子片断(376 bp),克隆入含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,构成受apoA-I启动子调控的重组报告基因质粒pGL3B-neo::apo,稳定转染人肝癌细胞株HepG2。
5)LTR-Luc luciferase geneLTR-Luc荧光素酶基因
6)luciferase report gene荧光素酶报告基因
1.Methods The +495bp~+584bp of SCN5A promoter was amplified by polymerase chain reaction(PCR)and recombinated into pGL3-promoter luciferase report gene vector and transient transfected HEK293 cell and H9C2 cell.方法应用聚合酶链反应扩增大鼠心肌SCN5A基因启动区+495bp~+584bp片段,与荧光素酶报告基因载体pGL3-promoter重组,以pGL3-promoter空载体为对照,瞬时转染人胚肾母细胞(HEK293)和小鼠胚胎心肌细胞(H9C2),检测荧光素酶活性。
2.coli chromosome,the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology.为了应用Red重组工程技术实现外源基因在大肠杆菌染色体上的表述,寻找染色体上外源蛋白的稳定高效表达位点,使用Red重组工程系统和kan/sacB无痕迹修饰技术,将易于定量分析的荧光素酶报告基因替换DY330染色体lac操纵子中的lacZ基因。
延伸阅读
可溶性荧光素 ,荧光素钠药物名称:荧光素钠英文名:Fluorescein Sodium别名: 可溶性荧光素 ,荧光素钠外文名:Fluorescein Sodium 适应症: 1.滴眼液用于眼科诊断,正常角膜不显色,异常角膜显色。 2.针剂用于测血液循环时间,静注后,在紫外线灯下观察,以10~16秒内唇部粘膜能见到黄绿色荧光为正常 用量用法: 1.滴眼后于角膜显微镜下观察颜色。 2.测血循环时间,于臂静脉注2ml,每次用量0.4~0.8g(2~4ml) 注意事项: 滴眼液应注意灭菌并防止污染 规格: 滴眼液:2% 注射液:0.4g(2ml)类别:实验诊断用药
