1)shuttle expression vector穿梭表达载体
1.Construction of shuttle expression vector in eukaryocyte plant cells;Kringle5的真核植物细胞穿梭表达载体的构建
2.Development of a Shuttle Expression Vector, pFC9, for Cryphonectria Parasitica;一个可在板栗疫病菌中自主复制的穿梭表达载体
3.GFP labeling shuttle expression vector pW425et-GFP was constructed by inserting green fluorescent protein(GFP)based on erythromycin drug resistance gene /thyA gene dipl-bolting pressure shuttle expression vector pW425et.以红霉素耐药性基因/thyA基因双筛选压力大肠杆菌—乳酸杆菌穿梭表达载体pW425et为基本骨架,将绿色荧光蛋白(GFP)基因插入该载体,构建GFP标记的穿梭表达载体pW425et-GFP。
英文短句/例句
1.Construction and Use in Biotin Systhase Gene Expression of an Expressional Shuttle-Vector pYG43;穿梭表达载体pYG43的构建及在生物素合成酶基因表达中的初步应用
2.Construction of a novel expression vector which can produce human IL-10 in both Escherichia coli and Bifidobacterium longum大肠埃希菌—双歧杆菌穿梭表达载体的构建及白介素-10基因的表达
3.Development of a Shuttle Expression Vector, pFC9, for Cryphonectria Parasitica;一个可在板栗疫病菌中自主复制的穿梭表达载体
4.Construction and application of a novel shuttle expression vector based on haloarchaeal plasmid pSCM201极端嗜盐古菌质粒pSCM201衍生穿梭表达载体的构建及应用
5.Construction of GFP Labeling Shuttle Expression Vector and Preparation of Restitution Lactobacillus绿色荧光蛋白标记穿梭表达载体构建及重组乳酸菌制备
6.Construction and identification of adenoviral shuttle vector with radiation-inducible smac gene辐射诱导Smac表达腺病毒穿梭载体构建及鉴定
7.Construction of hepatitis B virus X gene mutants shuttle vector and its expression in yeast cells乙型肝炎病毒X变异体穿梭载体的成功构建及在酵母中的表达
8.Construction of Deinococcal bacteria-Escherchia coli shuttle vector and expression of Luciferase gene抗辐射菌属-大肠杆菌间的穿梭载体的构建及荧光基因的表达
9.Expression of the Capsid Protein VP1 Antigen and Construction and Identification of the Recombinant Adenovirus Shuttle Plasmid Containing the Capsid Protein VP1 of Enterovirus 71;肠道病毒71型VP1基因抗原的表达及其腺病毒载体穿梭质粒的构建与鉴定
10.Construction of Prokaryotic Expression Vector of the Enterotoxin Gene of Clostridium perfringens Type C产气荚膜梭菌肠毒素基因原核表达载体的构建
11.The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt链霉菌和大肠杆菌穿梭质粒载体的构建
12.Construction and Optimization of E.coli and B.Subtilis Shuttle Vector;大肠杆菌枯草杆菌穿梭载体的构建及优化
13.Prokaryotic Expression Plasmid Construction, Expression, Purification and Function Detection of Penetratin/NF-κB Antagonizing Polypeptide;穿膜肽/NF-κB拮抗多肽原核表达载体的构建、表达、纯化与功能检测
14.Construction and identification of the recombinant adenovirus shuttle vector carrying the human Toll-like receptor 2 extracellular domain gene人Toll样受体2细胞外区基因重组腺病毒穿梭载体的构建及鉴定
15.The Construction of Shuttle Vector between Zymomonas Mobilis and Escherichia Coli;运动发酵单胞菌和大肠杆菌间穿梭质粒载体的构建
16.Construction and Expression of Shuttle Plasmid for Helicobacter pylori Urea Channel Protein UreI Gene人幽门螺杆菌尿素通道蛋白UreI基因穿梭质粒的构建及表达
17.Study on Expression and Regulation of Drought-Induced Genes in Haloxylon Ammodendron;梭梭干旱诱导表达基因及其调控研究
18.Construction of Recombinant Plasmid pEGFP-VEGF165 in vitro and Expression in 293T CellsVEGF165真核表达载体的构建与体外表达
相关短句/例句
constitutive shuttle vector组成型穿梭表达载体
3)Escherichia coli-Streptomyces shuttle expression vector链霉菌-大肠杆菌穿梭表达载体
4)Shuttle vector穿梭载体
1.Construction of shuttle vector consisted of human autophagic gene hAPG12 and its expression in yeast;含人自噬基因hAPG12穿梭载体构建及其在酵母自噬中的作用
2.Construction of Deinococcal bacteria-Escherchia coli shuttle vector and expression of Luciferase gene抗辐射菌属-大肠杆菌间的穿梭载体的构建及荧光基因的表达
3.Then, the fragment containing PpsbA and rhTNF cDNA was linked with the shuttle vector PDC_8, and resulted in expressing vector PDC_TNF.应用DNA重组技术,将人肿瘤坏死因子(rhTNF)cDNA插到质粒PRL439的PpsbA启动子下游,得到中间载体PRL_TNF;再把PRL_TNF上含PpsbA和(rhTNF)cDNA的片段连到穿梭载体PDC_8上,构建成穿梭表达载体PDC_TNF。
5)shuttle vectors穿梭载体
6)shuttle vector pMG36e穿梭载体pMG36e
1.lactis shuttle vector pMG36e,resulting in the plasmid pMG36e-NisI.【方法】根据GenBank报道的乳链菌肽Nisin抗性基因nisI的序列,以pLEB590为模板,设计特异性引物,PCR扩增出nisI片段,经TA克隆、酶切鉴定及测序之后,亚克隆至大肠杆菌-乳酸乳球菌穿梭载体pMG36e中,重组子命名为pMG36e-NisI,再将pMG36e-NisI电转化至对Nisin敏感的乳酸乳球菌MG1363中。
延伸阅读
穿梭轧制穿梭轧制shuttle rolling ChUOnSUO ZhOZhi穿梭轧制(shuttle rolling)轧件在同一机架不同孔型间或同一机列不同机架上首尾交替往复穿行的轧制方法。这种轧制方法广泛用于初轧机、开坯机、轨梁轧机、大中型型钢轧机等横列式轧机上。在小型横列式轧机上生产直径(或边长)大于20mm的圆钢和方钢时,也常用穿梭轧制。穿梭轧制是一种传统的轧制方法,它的优点是:所用设备简单,造价低;生产灵活,适应性强,适于多品种小批量生产;无张力轧制,适于生产断面较复杂的产品;操作简单、调整方便。缺点是:各道次之间间隙时间长,生产率低;轧件长度和线材盘重受到限制;不便于实现自动化。 (成友义)
