1)gene slicing by overlap extension重叠延伸引物PCR
2)Overlap extension PCR重叠延伸PCR
1.1 as the template,the PSD-95 mutant genes were amplified by overlap extension PCR,and then were cloned into the plasmids of PSD-95-pcDNA3.1为模板,采用重叠延伸PCR法获得突变型PSD-95的局部或全长cDNA片段,并克隆至PSD-95-pcDNA3。
2.According to the amino acids sequence of OC-IΔD86 gene and Escherichia coli codon usage, we synthesized this gene by overlap extension PCR method with 7 oligonucleotides DNA fragments.根据OC-IΔD86基因序列,设计合成了7条寡核苷酸片段,通过重叠延伸PCR技术合成了OC-IΔD86基因,利用设计好的BamH I/Xho I酶切位点将OC-IΔD86基因克隆到原核表达载体pet21b中,在1mmol/L的IPTG诱导后5h,OC-I?D86融合基因在大肠杆菌中得到表达,表达产物处于可溶状态,其表达量占总蛋白的11。
3.How to create two separate site-specific mutagenesis in a DNA fragment using overlap extension PCR was explored.探讨如何利用重叠延伸PCR对同一靶DNA片段中的两个不同位点实施联合突变。
英文短句/例句
1.Overlap Extension PCR Based Site-Directed Mutants of CTV P23 Gene利用重叠延伸PCR定点突变衰退病P23基因
2.Gene of Human Adiponectin Cloning by PCR-driven Overlap Extension and Expression in Pichia Pastoris;人脂联素基因的重叠延伸PCR法克隆及其在毕赤酵母中的诱导表达
3.Construction of Xylose Metabolic Genes in Yeast by Overlap Extension PCR利用重叠延伸PCR技术在酿酒酵母中构建木糖代谢相关基因
4.An overlap extension PCR method used in isolation of long cDNAs of acetyl-CoA carboxylase from Arabidopsis重叠延伸PCR克隆拟南芥ACCase基因和植物表达载体构建
5.Site-directed Mutagenesis of Sumf 1 Gene of Mouse Based on Overlap Extension PCR and Construction of Eukaryotic Expression Vector重叠延伸PCR法构建小鼠Sumf1基因的定点突变真核表达载体
6.Cloning of Cysticercus cellulosae AgB Gene by Using Splicing Overlap Extension PCR Method and Expression in BHK Cells重叠延伸PCR法扩增猪囊尾蚴AgB基因及其在BHK-21细胞中的表达
7.Away into the distance stretched ridge after ridge of snow-clad peaks.冰雪复盖的山峰重重叠叠绵延不断地一直伸向远方。
8.A New Method of Site-directed Mutagenesis by Modifying Overlap Extension PCR改进重叠延伸法引入DNA定点突变的新方法
9.The Fusion-PCR added Self-Extension Process加入自我延伸过程的融合PCR程序
10.Synthesis of the Murine Rotavirus Antigen Gene VP4 by Overlapping PCR重叠PCR法合成轮状病毒抗原基因VP4
11.Point Mutant of OsAMT1.2 by Using Gene Splicing by Overlap Extension利用重叠PCR技术对OsAMT1.2进行定点突变
12.Study of Single Chain Fvs Splicing and Site-directed Mutagenesis Based on Optimal Overlap PCR优化重叠PCR法进行单链抗体基因扩增和点突变
13.Full-length of human papillomavirus type 18L1 gene optimized using the plant prefered condons and synthesized by overlapping PCR植物偏爱密码子优化HPV18L1全长基因的重叠PCR合成
14.To lie or extend over and cover part of.重叠位于或延至…上面且遮
15.Real-time Quantification of MiRNAs by RNA-tailing and Primer-extension RT-PCR in Gastric CarcinomaRNA加尾和引物延伸RT-PCR法实时定量检测胃癌中miRNAs的表达
16.extend in importance or range.在重要性上或者范围上延伸。
17.SYNTHESIS OF GENE ENCODING HEPATITIS B CORE BY OVERLAPPING PCR AND ITS EXPRESSION IN ESCHERICHIA COLI重叠PCR合成乙型肝炎病毒核心抗原基因及其在大肠杆菌中表达
18.Computing process and communication process overlap, so as to hide network delay.使计算过程和通信过程重叠,以隐藏网络时延。
相关短句/例句
Overlap extension PCR重叠延伸PCR
1.1 as the template,the PSD-95 mutant genes were amplified by overlap extension PCR,and then were cloned into the plasmids of PSD-95-pcDNA3.1为模板,采用重叠延伸PCR法获得突变型PSD-95的局部或全长cDNA片段,并克隆至PSD-95-pcDNA3。
2.According to the amino acids sequence of OC-IΔD86 gene and Escherichia coli codon usage, we synthesized this gene by overlap extension PCR method with 7 oligonucleotides DNA fragments.根据OC-IΔD86基因序列,设计合成了7条寡核苷酸片段,通过重叠延伸PCR技术合成了OC-IΔD86基因,利用设计好的BamH I/Xho I酶切位点将OC-IΔD86基因克隆到原核表达载体pet21b中,在1mmol/L的IPTG诱导后5h,OC-I?D86融合基因在大肠杆菌中得到表达,表达产物处于可溶状态,其表达量占总蛋白的11。
3.How to create two separate site-specific mutagenesis in a DNA fragment using overlap extension PCR was explored.探讨如何利用重叠延伸PCR对同一靶DNA片段中的两个不同位点实施联合突变。
3)overlapping extension PCR重叠延伸PCR
1.Cell culture-adaptive mutations were carried out by the method of overlapping extension PCR OE-PCR at the sites of E1202G T1280I and S2197P in the genes of ns3 and ns5a.方法:根据HCV全长基因组cDNA序列及突变位点设计引物,运用重叠延伸PCR法对ns3和ns5a基因的E1202G、T1280I和S2197P位点进行细胞培养适应性突变,将突变后的片段分别克隆入pBluescriptIIKS(+)、pRSET-A载体,经测序正确后,分别连入含有HCV全长cDNA的质粒的H/FL相应位置,置换原未突变片段,并进行PCR及酶切鉴定。
4)overlap extention PCR重叠延伸PCR
1.K88ac-STⅡ fused gene amplified by overlap extention PCR was cloned into T vector. 利用重叠延伸PCR技术将K88ac STⅡ融合基因克隆于T载体上,将重组基因质粒转化到受体菌DH10B中,蓝白斑筛选阳性菌落,通过PCR、NcoI/XcoI酶切后测序,与genbank报道的K88ac结构基因序列进行比对,证明所克隆的目的片段为K88ac STII融合基因。
5)splicing overlap extension PCR重叠延伸PCR
1.Methods: The VH-linker-VL, namely scFv was prepared by amplifying the VH and VL genes of plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension PCR(SOE PCR).方法:设计以SfiⅠ、NotⅠ为酶切位点、以(Gly4Ser)3为linker的2对引物,从抗人PAF单克隆抗体可变区基因的克隆载体中扩增VH和VL基因,用重叠延伸PCR在VH和VL基因间引入连接短肽,构建VH-linker-VL的scFv基因。
2.Methods The VH-linker-VL, namely scFv gene was prepared by amplifying the VH and VL genes of plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension PCR (SOE PCR).方法 从抗TfR单克隆抗体重链和轻链可变区基因的克隆载体 pGEM T VH和 pGEM T VL中扩增重链可变区 (VH)和轻链可变区 (VL)基因 ,用重叠延伸PCR的方法 ,在VH和VL基因间引入连接短肽 (Linker) ,构建VH Linker VL的单链抗体 (singlechainFv ,scFv)基因。
3.Then, the complete gene of AgB was cloned by splicing overlap extension PCR method using 35 nucleotides overlap between the upper and downer fragments and inserted into the vector pVAX1.采用重叠延伸PCR法(splicing overlap extension PCR method,SOE-PCR)把具有35个相同碱基的上下半段扩增为全长基因,并构建到真核表达载体pVAX1,将酶切、PCR、测序鉴定的阳性质粒经脂质体转染BHK-21细胞。
6)SOE PCR重叠延伸PCR
1.SOE PCR and its Application in Genetic Engineering;重叠延伸PCR技术及其在基因工程上的应用
2.【Method】 Based on the DNA sequence from GenBank,seven single-strand DNA were designed for amplification of SUMO4 through SOE PCR(gene splicing by overlap extension PCR).【方法】根据Gen-Bank提供的核酸序列,设计7条单链DNA,采用重叠延伸PCR方法,合成SUMO4基因编码区。
3.The tat41-101 and tat1-101 gene fragments, encoding Tat41-101aa and Tat1-101aa respectively, were amplified by PCR and used to generate fusion gene tat(B41-101N)by splic-ing by overlap extension PCR(SOE PCR).方法在HIV-1HXB2株天然Tat蛋白N-端添加Tat41-101位氨基酸(aa),采用PCR法从HIV-1HXB2株tat基因中扩增分别编码Tat41-101aa和Tat1-101aa的tat41-101和tat1-101两个基因片段,重叠延伸PCR法扩增其融合基因ta(tB41-101N),并构建其原核表达质粒pET32a-ta(tB41-101N),经双酶切及测序验证后,转化大肠杆菌BL21(DE3),IPTG诱导表达。
延伸阅读
重叠1.见"重迭"。
