RT-PCR,RT PCR英语短句,例句大全

RT-PCR,RT PCR
1)RT PCRRT-PCR
1.RT PCR was then performed.目的建立灵敏外周血人乳房珠蛋白(hMAM)表达 RT-PCR 检测方法,探索 hMAM 在乳腺癌诊治中的应用价值。
2.IgM positive tissues from 12 spontaneous abortions were studied by RT PCR for the data .方法用ELISA法检测749例有异常妊娠史的育龄妇女和305例正常对照组妇女RV抗体RV-IgM、IgG,同时检测其中361例自然流产少于3次和52例自然流产3次以上的育龄妇女,并结合RT-PCR技术对12例IgM阳性的自然流产组织进行检测,数据行卡方统计。
3.It was used in the RT PCR to amplify DEN RNA extracted from DEN infected mosquitos.方法利用标记地高辛(Dig)的登革病毒通用引物进行RT-PCR扩增蚊媒体内的病毒RNA,然后以反向斑点杂交法检测病毒扩增片段并进行分型。

英文短句/例句

1.Rapid Detection of Avian Influenza Virus by Diplex RT-PCR and Real-Time RT-PCR;禽流感病毒双重RT-PCR及荧光RT-PCR快速检测
2.Detection of Five Citrus Diseases by RT-PCR and Multiplex RT-PCR;五种柑橘病害的RT-PCR及多重RT-PCR检测研究
3.Development of a multiplex PCR/RT-PCR assay for detection of porcine reproductive and respiratory syndrome virus,porcine parvovirus,pseudorabies virus and porcine circovirus type 2多重PCR/RT-PCR技术检测PRRSV、PPV、PRV和PCV-2
4.Diagnosis Establishment Formulation of RT-PCR、Fluorescence Quantitative PCR and Mutiplex RT-PCR Detection for Japanese Encephalitis Virus;猪乙脑病毒RT-PCR、荧光定量PCR及多重RT-PCR诊断方法建立
5.Establishment of Nested RT-PCR and Real-Time Fluorescence RT-PCR Methods to Detect West Nile Virus;西尼罗病毒套式RT-PCR和实时荧光RT-PCR检测方法的建立
6.Research of RT-PCR and Multiplex RT-PCR for Detection of H5N1 Subtype of Avian Influenza Virus;H5N1亚型禽流感病毒RT-PCR及多重RT-PCR检测技术的研究
7.Establishment of Rapid Diagnostic Methods for Detection of West Nile Virus by Nest RT-PCR and TaqMan Real-time RT-PCR;西尼罗病毒巢式RT-PCR和荧光定量RT-PCR两种检测方法的建立
8.Studies on the Detection of Prunus Necrotic Ringspot Virus with Real-time FluorescenRT-PCR and Hybridization CaptureRT-PCR-ELISA;实时荧光RT-PCR和杂交诱捕RT-PCR-ELISA检测李坏死环斑病毒的研究
9.Detection of Bean pod mottle virus in soybean by one step assay of RT-PCR and real-time fluorescent RT-PCRRT-PCR和实时荧光RT-PCR一步法检测大豆中菜豆荚斑驳病毒
10.A QUICK AND MORE SENSITIVE METHOD TO IDENTIFY PORK IN PROCESSED AND UNPROCESSED FOOD BY PCR AND REALTIME-PCR METHOD高效快速检测食物中猪成分的PCR和RT-PCR方法
11.Detection of Impatiens necrotic spot tospovirus(INSV) by RT-PCR and Nested PCR凤仙花坏死斑病毒的RT-PCR和巢式PCR检测
12.Detection of hepatitis C virus by using a new europium fluorescent chelate as a labelEu标记RT-PCR检测丙型肝炎病毒RNA
13.One-step RT-PCR for detection of SARS associated coronavirus一步法RT-PCR检测SARS冠状病毒技术研究
14.Detecting HCV RNA by one-step short-product RT-PCR一步短片段RT-PCR方法检测hcv RNA
15.Quantitative Determination of IFN-γ mRNA by the Competitive RT-PCR Method竞争RT-PCR定量测定IFN-γmrna方法的建立
16.Establishment of the Diagnostic Method for the RT-PCR and ELISA of Vesicular Stomatitis;水泡性口炎的RT-PCR及ELISA诊断方法建立
17.Study on Diagnosis by Reverse Transcription Polymerase Chain Reaction and Antibody Detection of Hog Cholera;猪瘟RT-PCR诊断及抗体监测研究
18.Detection of Food-and-Mouth Disease by RT-PCR and Immune Antibody Surveillance;口蹄疫的RT-PCR检测及免疫抗体监测

相关短句/例句

reverse transcription-polymerase chain reactionRT-PCR
1.With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep.以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。
2.MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR).方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。
3.Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues.方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。
3)Reverse transcription polymerase chain reactionRT-PCR
1.Then the level of VEGF mRNA in kidney was determined by reverse transcription polymerase chain reaction (RT-PCR); the expression of VEGF and VEGF.【方法】取3月龄、12月龄大鼠作为对照组,24月龄大鼠作为观察组各6只,提取其肾脏组织的RNA,应用逆转录聚合酶链反应技术(RT-PCR)检测肾脏组织内VEGFmRNA转录水平,以及应用免疫组化技术检测肾小球VEGF和VEGFR蛋白的表达;应用酶联免疫法检测肾组织培养液的VEGF总含量。
2.Methods Reverse transcription polymerase chain reaction (RT PCR) was used to evaluate the amount of insulin like growth factor mRNA in exercise induced damaged skeletal muscle, and specimens were used for ultrastructural observation.方法利用IGF引物对不同损伤程度骨骼肌标本进行RT-PCR检测,同时观查损伤骨骼肌超微结构改变。
3.Methods NIT-1 cells were exposed to cyclosporin A (10 μmol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction.放射免疫测定法(RIA)检测胰岛素释放,半定量RT-PCR检测CsA处理NIT-1胰岛β细胞后线粒体氧化磷酸化酶系中的几种主要成员(Nuox23、Cox7c和Atp5K)在mRNA水平上的表达。
4)RTPCRRT-PCR
1.Expression of cbfa1 gene in rat osteosarcoma UMR106 cell was detected by RTPCR after the 72?h s treatment of FSK88 liquid with different mol concentration(25,50,100?μmol/L),Retinoic Acid(RA,10?μmol/L) and mixed liquid of FSK88 and RA(1∶1).以大鼠成骨肉瘤细胞———UMR106为模型,应用RT-PCR方法,检测用不同浓度的FSK88药液(25,50,100μmol/L)、维甲酸(RA,10μmol/L,阳性对照)以及FSK88+RA(等体积)混合药液处理细胞72 h后,细胞内cbfa1基因表达量的变化。
2.by RTPCR and 3′-RACE technology.]实生苗为材料,采用RT-PCR及RACE技术,获得了PPF-1(开花、衰老相关基因)同源基因的cDNA全长序列PtPPF-1,该cDNA全长1 493 bp,编码452个氨基酸,与豌豆PPF-1、拟南芥ALB3、水稻PPF-1及葡萄一未命名基因的氨基酸序列同源性较高,说明PPF-1基因在进化过程中可能变异较小。
5)RT PCRRTPCR
6)real time RT-PCR实时RT-PCR
1.To establish the real time RT-PCR method to measure the expression of c-erbB-2 mRNA in breast cancer tissues of beside tumors and tissues of adenosis of mammary glands, to find out the relationship of c-erbB-2 protein.建立LightCycler实时RT-PCR方法检测乳腺癌、癌旁组织及乳腺腺病组织c-erbB-2基因mRNA的表达。