PCR-TRAP端粒酶重复序列扩增法
1.Methods Telomerase activity was measured by PCR-TRAP in cancerous lesions brushing cells from patients with lung cancer and lesions brushing cells from patients with noncancerous pulmonary disorders;CEA in bronchial washing fluid and in blood was measured by radioimmunoassay ,in which the specimens were obtained by fiberoptic bronc.方法 采用端粒酶重复序列扩增法和放免法测定分别检测肺癌病变组织 (12 0例 )、非癌性肺疾病 (30例 )纤支毛刷刷脱细胞的端粒酶活性和毛刷涮洗液、血CEA浓度 ,并和病理学及细胞学检查结果进行比较。
3)telomerase repeat amplification protocol (TRAP)端粒重复序列扩增技术
1.Methods: Telomerase repeat amplification protocol (TRAP) assay was used to examine telomerase activity in forty-eight brain gliomas and eight normal brain tissues.方法 :应用端粒重复序列扩增技术 (TRAP)对 4 8例胶质瘤标本和 8例正常脑组织的端粒酶活性进行检测。
4)Telomeric repeat amplification protocol端粒重复序列扩增
1.Telomerase activity was examined in 25 glioblastomas by telomeric repeat amplification protocol-silver staining.[方法]采用端粒重复序列扩增 -银染法检查了25例胶质母细胞瘤的端粒酶活性。
5)telomeric repeat amplification protocol-enzyme linked immunosorbent assay酶联免疫端粒重复序列扩增法
6)Real-time fluorescent quantitative TRAP实时荧光定量端粒重复序列扩增法
延伸阅读
分子扩增分子式：CAS号：性质：又称无性繁殖，克隆繁殖，分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质，即DNA大分子提取出来，在离体条件下进行切割后，取其片段，或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来，然后再导入某一受体细胞中，让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始，它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。
