能够抑制农杆菌生长的大豆花叶病毒序列及其应用的制作方法

本发明属于基因工程领域，涉及能够抑制农杆菌生长的大豆花叶病毒序列及其应用。

背景技术

农杆菌属于细菌界变形菌门根瘤菌目根瘤菌科，是一种革兰氏阴性菌。农杆菌能够将自身的一段dna序列转移并插入到植物的dna中，这使其成为一种重要的植物转基因工具，广泛应用于植物的遗传转化和瞬时表达实验中。然而，农杆菌很少直接用作重组克隆筛选的宿主菌。农杆菌侵染实验中所使用的重组克隆通常需要事先在大肠杆菌等其他微生物中筛选鉴定，这不仅使实验过程过于繁琐，更是增加了实验的耗时。

重组克隆筛选技术是现代分子生物科学研究的基础技术，在几十年的发展中得以快速完善。重组克隆构建筛选的常用策略有两类。第一类基于抗生素抗性基因的正筛选，这类基因可以使携带含有抗生素抗性基因质粒的微生物在含有相应的抗生素平板上生长，从而能够筛选到含有重组质粒的单菌落；第二类基于抑制微生物生长的基因的负筛选，这类基因通常预先连接到目标载体上，通过外源片段与之交换形成新的不含有生长抑制基因的重组克隆，从而在生长出的菌落里获得正确重组的克隆。第二类策略需要同第一类策略联用来实现重组克隆的构建筛选，这方面应用比较成功的为英潍捷基的gateway载体构建法(利用ccdb基因实现重组克隆的筛选)，这个方法也是应用较早的不必使用内切酶的载体高效构建方法。但是ccdb基因不能抑制农杆菌的生长，也不能用于在农杆菌中进行重组克隆的筛选。

大豆花叶病毒属于马铃薯y病毒属病毒，基因组编码11个成熟蛋白(p1,hc-pro,p3,p3n-pipo,6k1,ci,6k2,nia-vpg,nia-pro,nib和cp)，因其能够侵染大豆并在叶片上形成花叶症状而得名。在中国，我们利用10个鉴别寄主将大豆花叶病毒不同分离物划分为sc1-sc21共21个株系。大豆花叶病毒整个生活史大部分阶段为在植物体内进行复制、装配和运输，其传播可以依赖同蚜虫体内受体蛋白的互作来实现。但是，至今没有关于马铃薯y病毒属病毒与农杆菌互作的报道。

技术实现要素：

本发明的目的是提供能够抑制农杆菌生长的大豆花叶病毒序列。

本发明的另一目的是提供该序列在基因工程方面的应用。

本发明的目的可通过如下技术方案实现：

能够抑制农杆菌生长的大豆花叶病毒基因，包含如seqidno.2所示的核苷酸序列。

本发明所述的大豆花叶病毒基因在抑制农杆菌生长中的应用。

所述的大豆花叶病毒基因优选自seqidno.2或seqidno.1所示的核苷酸序列。

我们构建了3个smv的病毒克隆载体。将这3个病毒克隆载体转化到农杆菌eha105菌株，转化两天之后，转有pcb301-sc3(#sc3)和pcb301-sc7(#sc7)的农杆菌可以正常生长，然而转有pcb301-sc15(#sc15)的农杆菌却未见生长(图1)。为了得到能够抑制农杆菌生长的最短序列，我们构建了一系列包含不完整sc15的克隆载体(图2)并转化农杆菌。我们发现能够抑制农杆菌生长的基因序列包含编码p1,hc-pro,p3,p3n-pipo,6k1,ci,6k2,nia-vpg,nia-pro,nib的序列，截短后会导致抑制能力的下降。

所述的大豆花叶病毒基因在基因工程中的应用。

所述的大豆花叶病毒基因在构建重组克隆受体载体中的应用。

所述的应用，优选将所述的大豆花叶病毒基因预先构建到载体中作为受体载体，然后将目标基因片段与受体载体进行重组反应，实现目标基因片段仅直接转化农杆菌就能够进行重组克隆的目的。

本发明所述的大豆花叶病毒基因优选自seqidno.2或seqidno.1所示的核苷酸序列。

本发明设计了基于lr反应的克隆重组及筛选实验。首先我们构建了含有sc15p的用于lr反应的载体pcb301-attl-sc15p(图3a)，随后，我们将pcb301-attl-sc15p分别和pdonor-ci及pdonor-avh241进行lr反应。反应液直接转化农杆菌，两天后对长出的单菌落进行菌液pcr检测，检测结果表明所有挑取的单菌落含有的都是正确重组的克隆(图3b)。证明sc15p能够用于在农杆菌中直接实现重组克隆的筛选。

有益效果：

本发明公开的能够抑制农杆菌生长的大豆花叶病毒序列是首次有关病毒序列能够抑制农杆菌生长的发现，并且该序列对农杆菌的抑制能力极其显著，具有很大的潜在应用价值。

本发明提出并以实验证实了能够抑制农杆菌生长的大豆花叶病毒序列能够用于在农杆菌中直接实现重组克隆的筛选，并且筛选正确重组克隆的效率达到100％，实用性表现优异。

利用本发明直接在农杆菌中实现对重组克隆的筛选，有助于我们在农杆菌相关的遗传转化实验中跳过利用大肠杆菌筛选重组克隆的步骤，实现实验进程的快速推进。

附图说明

图1pcb301-sc15对农杆菌生长的抑制现象；a：病毒克隆载体的结构，显示lb与rb之间的元件；b：转化pcb301-sc3、pcb301-sc7和pcb301-sc15后的农杆菌生长状况，标尺长度为4mm；

图2#sc15截短后转化农杆菌后的生长情况；a：#sc15截短位置及转化农杆菌后的生长情况图示，+表示农杆菌能够生长，—表示农杆菌无法生长；b：截短sc15病毒克隆载体转化农杆菌生长情况照片，#s1至#sc15中标尺长度为4mm，#s4l图为#s4细节图，标尺长度为0.4mm；

图3sc15农杆菌生长抑制序列在筛选重组克隆中的应用；a：pcb301-attl-sc15p载体图示，显示lb与rb之间的元件；b：各载体转化农杆菌后的生长情况照片。其中a为pcb301-attl-sc15p，b、d分别为pdonor-ci和pdonor-avh241，c、e分别为pdonor-ci和pdonor-avh241与pcb301-attl-sc15p进行lr反应之后的产物转化农杆菌生长结果，标尺长度为4mm；c：农杆菌菌落pcr检测，c为b中c平板菌落pcr结果，e为b中e平板菌落pcr结果，其中泳道m为dnamarkerdl5000，泳道n为阴性对照，其它泳道为不同单菌落为模板的菌落pcr结果。

具体实施方式

实施例1：大豆花叶病毒中农杆菌生长抑制序列的发现

1、大豆花叶病毒克隆载体的构建

我们从新鲜的smv侵染的大豆叶片中使用trizol提取总rna；总rna使用takara的primescript^tmii1ststrandcdnasynthesiskit反转录为cdna。使用cdna为模板，我们将smv分为5段扩增并纯化回收。回收后的上述各个pcr片段(如1a、2a、3a、4a、5a五个片段)直接与使用stui和smai线性化的pcb301载体共同转化clontech公司的酵母菌株y2hgold，并使用sd/-trp培养基筛选重组克隆。从酵母单菌落中提取的质粒使用pcr及测序进行验证后进行质粒的扩繁。构建成功的病毒克隆分别命名为pcb301-sc3(#sc3)、pcb301-sc7(#sc7)和pcb301-sc15(#sc15)。各pcr片段获得策略如下：1a、2a、3a、4a、1b、2b、3b、4b、1c、2c、3c和4c片段的获得：使用相应的大豆花叶病毒sc3\sc7\sc15的cdna为模板使用表1中对应引物进行普通pcr程序进行即可；5a片段的获得：首先使用sc3的cdna为模板，使用引物smv-frag5a-f和smv-frag5a-r-1进行pcr得到第一次pcr产物，然后使用第一次pcr产物作为模板，使用smv-frag5a-f和smv-frag5a-r-2进行pcr得到第二次pcr产物，最后使用第三次pcr产物作为模板，使用smv-frag5a-f和smv-frag5a-r-3进行pcr得到5a片段；5b片段的获得：方法与5a片段的获得一致，只需将第一次的模板换成sc7的cdna；5c片段的获得：方法与5a片段的获得一致，需要将第一次的模板换成sc15的cdna，且第一次pcr引物换成smv-frag5a-f(6067)和smv-frag5a-r-1。构建各载体所用引物的序列见表1。

从酵母单菌落中提取的质粒的pcr验证方法：使用质粒为模板，使用构建相应病毒载体时扩增5个片段所用的引物进行pcr(5a、5b和5c片段的扩增使用第三次pcr的引物，即smv-frag5a-f和smv-frag5a-r-3)，所有片段大小符合预期即可。

pcr体系及程序：每个反应50μl：模板1μl，上下游引物各1μl(浓度10μm)，双蒸水9.5μl，primestarmaxdnapolymerase(takara公司)25μl。程序：预变性：95℃，2min；30个循环：变性98℃，5sec，退火57℃，15sec，延伸72℃，1min30sec；最后降温到12℃。

本实例所用的smv保存于国家大豆改良中心，其基因组序列分别对应genbank中的sc3(jf833015.1)、sc7(kt285170.1)和sc15(jf833015.1)。

表1构建载体所用引物

2、各克隆载体转化农杆菌及农杆菌生长抑制现象的发现

我们使用上述构建成功的大豆花叶病毒克隆载体转化农杆菌菌株eha105，转化方法为冻融法，转化后的菌液于28℃复苏2小时后涂板于直径9cm的yep培养基平板上(含有50ng/ml的kan和25ng/ml的rif)在28℃的恒温箱中培养48小时，并使用体视显微镜拍照。观察发现，转有pcb301-sc3(#sc3)和pcb301-sc7(#sc7)的农杆菌可以正常生长，然而转有pcb301-sc15(其中#sc15基因序列如seqidno.1所示)的农杆菌却未见生长(图1)。

pcb301-sc15中抑制农杆菌生长的具体序列的明确

为了得到能够抑制农杆菌生长的最短序列，我们构建了一系列包含不完整sc15的克隆载体(#s1至#s12)(图2a)。#s1构建过程：首先以#sc15的质粒作为模板，使用引物hcpro-recf和pcb301-rec-r进行pcr扩增获得片段1g并纯化回收，然后将回收后的1g片段与使用stui和smai线性化的pcb301载体进行同源重组反应，反应产物转化大肠杆菌dh5α并涂含有卡那霉素的lb平板培养基上培养16小时。最后挑取单菌落摇菌提取质粒，使用引物hcpro-recf和pcb301-rec-r检测片段是否正确插入。#s2至#s12的构建过程和#s1一致。同源重组所用的试剂盒为南京诺唯赞公司的clonexpress-iionestepcloningkit试剂盒，操作说明见说明书。构建各载体所用引物见表2。观察转化#s1与#s2转化结果发现只要p1序列被删除，克隆载体就不能再抑制农杆菌生长，这进一步验证了上面的实验结果(图2b)。观察转化#s3、#s10、#s11、#s12的农杆菌发现，utr序列和cp序列的删除并没有影响到克隆载体对农杆菌的抑制，说明utr和cp序列不是抑制农杆菌生长所必须的。观察转化#s4至#s9，我们发现随着3’端病毒序列的截短，农杆菌生长越来越明显，抑制能力显著下降(图2b)。我们将#s3中能够够抑制农杆菌生长的较小序列命名为sc15p，序列如seqidno.2所示。

表2构建载体所用引物

实施例2：sc15农杆菌生长抑制序列在高效筛选重组克隆中的应用

1、pcb301-attl-sc15p载体的构建

我们将pcb301载体lb和rb之间的序列替换成attl1及attl2序列，并在attl1和attl2序列之间插入能够抑制农杆菌生长的较小片段sc15p，构建成为的载体命名为pcb301-attl-sc15p(图3a，pcb301-attl-sc15p全序列见seqidno.3)。pcb301-attl-sc15p的构建过程如下：首先用pgwb5质粒为模板，使用引物attl1-301rec和attl2-301rec进行pcr扩增获得片段1s，用pcb301质粒为模板，使用引物301-frag-f和301-frag-r进行pcr扩增获得片段2s，将纯化后的1s和2s片段通过南京诺唯赞公司的clonexpress-iionestepcloningkit试剂盒进行连接并转化大肠杆菌dh5α，然后挑取单菌落摇菌并提取质粒获得载体pcb301-attl-ccdb；然后用pcb301-attl-ccdb质粒为模板，使用引物attr1和attr2进行pcr扩增获得片段1t，用#sc15质粒为模板，使用引物nib-attr2-recr和p1-attr1-recf进行pcr扩增获得片段2t，将纯化后的1t和2t片段通过南京诺唯赞公司的clonexpress-iionestepcloningkit试剂盒进行连接并转化大肠杆菌dh5α，然后挑取单菌落摇菌并提取质粒获得载体pcb301-attl-sc15p。转化农杆菌表明，pcb301-attl-sc15p依然能成功抑制农杆菌的生长(图3b)。

2、pcb301-attl-sc15p与pdonor-ci、pdonor-avh241之间的lr反应

各取pcb301-attl-sc15p体积2μl分别和pdonor-ci及pdonor-avh241各2μl混匀，然后加入lr重组酶，放入25℃金属浴中1小时进行lr反应，反应产物待用。其中pdonor-ci和pdonor-avh241为使用载体pdonor-zeocin分别与smv株系sc4的ci蛋白序列和大豆疫霉菌的avh241效应因子序列进行bp重组反应构建的载体。pdonor-ci和pdonor-avh241具体构建过程如下：分别取被sc4株系及大豆疫霉菌侵染的大豆组织提取总rna，然后分别反转录为cdna得到用于pcr的模板。其中1u片段的扩增使用引物attb1-ci-f及attb1-ci-r；1v片段的扩增使用引物attb1-avh241-f及attb2-avh241-r。扩增后的1u及1v分别纯化回收后与载体pdonor-zeocin进行bp重组反应。反应产物转化大肠杆菌dh5α后涂含有zeocin的lb平板，培养16小时候挑取单菌落摇菌并提取质粒，最后使用m13通用引物进行pcr验证及测序验证。上述smv株系sc4及大豆疫霉菌保存于国家大豆改良中心，构建各载体所用引物见表3。

表3构建载体所用引物

3、lr反应产物转化农杆菌及单菌落的检测

我们将lr反应产物取出3μl加入农杆菌感受态eha105中进行转化实验，没有进行lr反应的各质粒转化农杆菌作为对照。转化使用冻融法，转化后的菌液于28℃复苏2小时后涂板于直径9cm的yep培养基平板上(含有50ng/ml的kan和25ng/ml的rif)在28℃的恒温箱中培养48小时，并使用体视显微镜拍照(图3b)。从长出的单菌落中各挑取20个单菌落用于菌落pcr扩增(所用引物为表3中的attl1-301rec及attl2-301rec)，扩增产物跑胶检测。检测结果表明所有挑取的单菌落含有的都是正确重组的克隆(图3b)。这一结果也通过测序验证。

序列表

<110>南京农业大学

<120>能够抑制农杆菌生长的大豆花叶病毒序列及其应用

<160>3

<170>siposequencelisting1.0

<210>1

<211>16295

<212>dna

<213>大豆花叶病毒(soybeanmosaicvirus)

<400>1

gtctggccgtccttctcgtacttggtattccgaatcttgccctgcacgaataccagcgac60

cccttgcccaaatacttgccgtgggcctcggcctgagagccaaaacacttgatgcggaag120

aagtcggtgcgctcctgcttgtcgccggcatcgttgcgccacatctaggtactaaaacaa180

ttcatccagtaaaatataatattttattttctcccaatcaggcttgatccccagtaagtc240

aaaaaatagctcgacatactgttcttccccgatatcctccctgatcgaccggacgcagaa300

ggcaatgtcataccacttgtccgccctgccgcttctcccaagatcaataaagccacttac360

tttgccatctttcacaaagatgttgctgtctcccaggtcgccgtgggaaaagacaagttc420

ctcttcgggcttttccgtctttaaaaaatcatacagctcgcgcggatctttaaatggggt480

gtcttcttcccagttttcgcaatccacatcggccagatcgttattcagtaagtaatccaa540

ttcggctaagcggctgtctaagctattcgtatagggacaatccgatatgtcgatggagtg600

aaagagcctgatgcactccgcatacagctcgataatcttttcagggctttgttcatcttc660

atactcttccgagcaaaggacgccatcggcctcactcatgagcagattgctccagccatc720

atgccgttcaaggtgcaggacctttggaacaggcagctttccttccagccatagcatcat780

gtccttttcccgttccacatcataggtggtccctttataccggctgtccgtcatttttaa840

atataggttttcattttctcccaccagcttatataccttagcaggagacattccttccgt900

atcttttacgcagcggtatttttcgatcagttttttcaattccggtgatattctcatttt960

agccatttattatttccttcctcttttctacagtatttaaagataccccaagaagctaat1020

tataacaagacgaactccaattcactgttccttgcattctaaaactttaaataccagaaa1080

acagctttttcaaagttgttttcaaagttggcgtataacatagtatcgacggagccgatt1140

ttgaaaccacaattatgggtgatgctgcaactcgagcgggccgggagggttcgagaaggg1200

ggggcaccccccttcggcgtgcgcggtcacgcgcacagggcgcagccctggttaaaaaca1260

aggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaaaaac1320

gggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaat1380

aggtgcgcccctcatctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcat1440

ctgtcagtagtcgcgcccctcaagtgtcaataccgcagggcacttatccccaggcttgtc1500

cacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgaggctggc1560

cagctccacgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggt1620

gagtcggcccctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttcc1680

gcgaggtatccacaacgccggcggccgcggtgtctcgcacacggcttcgacggcgtttct1740

ggcgcgtttgcagggccatagacggccgccagcccagcggcgagggcaaccagcccggtg1800

agcgtctctagtggactgatgggctgcctgtatcgagtggtgattttgtgccgagctgcc1860

ggtcggggagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacgc1920

ttagacaacttaataacacattgcggacgtttttaatgtactggggtggttttaagcttg1980

catgcctgcaggtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaag2040

atacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaa2100

acctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaagg2160

aaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcct2220

ctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaag2280

acgttccaaccacgtcttcaaagcaagtggattgatgtgataacatggtggagcacgaca2340

cacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgaga2400

cttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtc2460

actttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgata2520

aaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccac2580

ccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggatt2640

gatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacc2700

cttcctctatataaggaagttcatttcatttggagaggaaattaaaactactcataaaga2760

caacaaacaattcaagcgcaaacagaaattttcgtaactacatttctacaagcaaccatt2820

actccagctatttgcagtttcacattttctcacagcagcagtaagtcaaatggcaacaat2880

catgattggaagcatggcgatctctgtgccaaacactcacgtcttgtgcgcatcgaattc2940

tgtgatgccggttcaagcagttcagatggcaaaacaagtgccttccgctcgaggggtgtt3000

atacacacttaaaagagagggcaacacgcaagtgcataagcacgaggaggcgttgcgcaa3060

atttcaagaagcattcgaccaagatgttgacattcagcgaaggcttttggtaaacaagca3120

tagttccatacaatccacaaagaagaatggtttaaccttgcgtcgcttaactttagagca3180

ggctcgagcaaaagaagcggcaattgcaaggcgaaggcaagaagaggaagactttctcaa3240

tgggaaatatgaacagcaattttatgctggtgtttccgctacaaagtccatgaaatctga3300

aggagggagtgttggattcagaacaaaatactggagaccaactccaaagaagactaaaga3360

aaggcgtgcaacatcgcagtgtaggaaaccaacatatgttttggaggaggttctttccat3420

agcttcaaggagtggtaagctggttgaatttatcacaggaggcaaaggaaagagagtcaa3480

aatctgttatgtgcgcaagcatggcgcaatattgcccaagttctccctcccgcatgaaga3540

aggcaaatatatccatcaggagcttcagtatgcaagcacatatgagtttcttccttatat3600

ttgcatgtttgcaaaatataagagcataaatgcggatgatataacttatggagatagtgg3660

tttactgtttgatgagcgatcatctttaaccacaaatcatactaagttaccgtactttgt3720

cgttcggggaaggaagaatgggaagcttgttaacgctcttgaagtggttgaaaacatgga3780

tgatattcagcactactcccaaaatcctgaagctcagtttttccgtggttggaaaaaggt3840

gtttgataaaatgcctcctcatgtggagaatcatgaatgcaccattgatttcacaaatga3900

gcaatgtggtgaattggcagcggctataagccaatcaatttttccagttaagaaactatc3960

atgtaagcaatgtcggcagcacattaagcacctcagttgggaagagtataaacaattcct4020

cctggctcatatgggctgccatgggactgaatggaaaactttccaagaaattgatggcat4080

gaagtatgtaaagagagtgattgagacatcaactgcggaaaacgcaagtctgcagacatc4140

actggagattgtgcgtttaacgcagaactataagagcactcacatgcttcaaatacagga4200

cattaataaggccctgatgaagggtccatcggtaacacagagcgagctggagcaagcttc4260

caagcagctgctcgcaatgacacaatggtggaagaatcacatggctttgactgatgaaga4320

tgcacttaaagtgttcaggaataagagatcttccaaagcactactcaacccaagtttact4380

ttgtgataaccagttggacaagaatggtaattttgtttggggagagcgtggcaggcattc4440

aaagcgattctttgcgaattattttgaagaggtggttccttctgaagggtatagtaagta4500

tgtgatcagaaagaatccaaacggacaaagggagttggcaattgggtcacttattgtacc4560

gttgaatttcgagcgcgctcgaatggcattacagggcaagagcgtaccaagaaagccaat4620

tacactgtcatgtatctcaagacaagacggaaactttgtatatccttgttgttgtgtcac4680

acatgatgatggaaaagctttctattctgagctcaagagtcctacaaagcgccatttggt4740

tattggaacgtcaggtgacccgaagtacattgatctaccagccactgatgcagacaggat4800

gtacatagctaaagaaggattttgttatcttaacatcttcttggcaatgttggttaatgt4860

aaatgaagatgaggccaaggacttcacaaagatggtgagggatgttattataccacagtt4920

aggaaagtggccgacaatgttagatgtagcaacagctgcatacatgctaacagtttttca4980

ccctgagaccaggaatgccgagctcccacgtattttagttgaccatgcgtgtcaaaccat5040

gcatgtgattgactcttttggatccttgacagttgggtaccatgttcttaaagctggtac5100

agtgaatcaattaattcaatttgcttctaatgaccttcagagtgagatgaaattctacag5160

agttggtggtgaagtgcaacagagaatgaagtgtgaaacagcacttataacaagcatttt5220

caaacctaagagaatggttcaaatccttgaaaatgacccatacattctcttgatgggctt5280

ggtttcaccttctatcttgattcacatgtatcgtatgaagcattttgagaaaggggtgga5340

gttgtggataagtaaagaacatagtgtggcaaagattttcatcatattggaacaactcac5400

taagagggtcgctgcaaatgatgtgctacttgagcaacttgaaatgatttcagaaacttc5460

tgagaggttcatgagtatcttagaggattgccctcaagcaccacattcatacaagacggc5520

aaaagatttgttgacaatgtacatagaaaggaaagcatccaatagccaattggtggagaa5580

tggttttgtagatatgaatgacaaattgtacatggcatatgaaaaaatctactcagatcg5640

cttgaagcaggaatggcgcgcattaagctggttggaaaaattttctacaacatggcaatt5700

gaaaagatttgctccacatacggagaaatgtttgacaaagaaagttgtagaagaaagcag5760

cgcatcttcaggaaactttgcgagtgtgtgcttcatgaatgcccagtcacacctaagaaa5820

tgtaagaaatacactttttcaaagatgtgaccaggtttggacagcatcggtgcgagcctt5880

tgtgaggttcataatttcaacacttcacagatgctacagtgatatagtttatctggtgaa5940

catctgtataatcttttcattgctcgtccaaatgactagtgtgcttcagaacattgtcaa6000

cacagcaaggagagacaaagcactcttgaatggatggaaaaggaaagaagatgaggaggc6060

cgtgattcatttgtatgaaatgtgtgaaaagatggaaggtggacatccgagtattgagaa6120

atttttggaccatgtcaagggagtcagacccgatctactccctatagcagtgagcatgac6180

aggacaatcagaggatgtctccgcacaggccaaaacagtaactcaattacaacttgagaa6240

aatcgtggcattcatggctttgttgaccatgtgtattgataatgaaaggagtgatgcggt6300

tttcaaagtattgagtaagttgaaggcatttttcagcacaatgggtgaggatgttaaagt6360

gcagagtctcgatgagattcaaaatattgatgaagataaaaagctcacaattgatttcga6420

ccttgaaacgaataaggagtcttccagtgtctcttttgatgtcaaatttgaagcctggtg6480

gaatagacagttggaacagaatagagtaattccacactacaggtcgacaggtgagtttct6540

ggaattcacaagagaaacagcagccaagattgcaaatttggtagctacttcaagccacac6600

agaatttttgattagaggtgcagttggctcagggaaatcaacaggtttaccacaccacct6660

ttcaaagaagggcaaagttctgctactggagccaactagaccattagcagaaaatgtcag6720

taagcagttgagccttgaacctttctatcacaatgtaacactgagaatgagagggctgag6780

taagtttggttcaagcaacatagttgtcatgacaagtgggtttgcgtttcattactatgt6840

taacaatccacaacagttatctgatttcgattttatcataatagatgaatgccatgtgca6900

agatagcccaacgattgcattcaactgtgcgcttaaagagtttgaattcagtggcaagct6960

tataaaagtgtctgcaacacctccagggagagagtgcgagttcacaacgcagcatccagt7020

gaagttgaaggttgaagaccacttatcttttcagaactttgtgcaagctcaaggtacagg7080

atcaaatgctgatatgatccaacatgggaacaacttacttgtatatgttgcaagctacaa7140

tgaagttgaccaattgtcacgattattaactgaaaaacattacaaggtgacaaaggttga7200

tgggagaacaatgcaaatgggaaatgtagagattgcaaccacaggcacggaaggaaaacc7260

acacttcatagtcgcaacaaacatcattgagaatggagtgactcttgatattgattgtgt7320

aattgattttggacttaaagtggtggctactcttgatacagataaccggtgtgtgcgtta7380

caacaaacagtcagtttcttatggagagcgaattcagagacttggcagagttggtcgttg7440

taaacctgggtttgcgcttaggattggacacacgggaaaaggagttgaggaagttcccga7500

gttcatagctacagaggcagcctttctatcctttgcttatgggttgccagttacaacaca7560

gagtgtctcgaccaatatactgtcccgttgcacagtaaaacaagctcgagtggctttgaa7620

ttttgagctaactccattcttcaccaccaacttcataaagtatgatggtagcatgcaccc7680

agaaattcacagattgctcaagtcctataaactcagggagtctgagatgttgttgaccaa7740

gctagccataccatatcagtttgttgggcagtggataacagtcaaggaatatgaacgtca7800

aggtgtccaccttaattgcccagagaaagtgaaaatacctttctatgtgcatggaatacc7860

agacaagttgtatgagatgttgtgggacacagtttgtaaatacaagaatgatgctggatt7920

cggctcaattaagagtgtgaatgcaacgaagattagttacactctaagcactgatccgac7980

agcaattcctcgcacacttgcaatactggaccatttactgagcgaggaaatgactaagaa8040

gagtcattttgacacaattggctctgctgtcactgggcattccttttctcttgcaggtat8100

agctgacggaattagaaagaggtacttgaaggactacacacagcacaatatagccgtctt8160

acaacaggctaaagcacagctgctagaatttgactgcaacaaagttgacatcaacaacct8220

gcacaatgttgagggtataggcattttaaatgcagttcaactacagagcaagcaagaggt8280

gagcaaatttttgcagcttaaaggaaagtgggatggaaagaaattcatgaatgatgctgt8340

agtggctatcttcactttagtgggcggtggttggatgttatgggattacctcacaagagt8400

tatacgtgaaccagtatcaactcaaggaaagaagaggcaaatacaaaagctcaaattcag8460

ggatgcctttgacagaaaagtaggccgtgagatatacgcggatgattacaccatggagca8520

tacctttggggaggcatacaccaagaaaggaaagcagaaaggtagcactcgtacaaaagg8580

gatgggtcgcaagtcgaggaacttcatacatctgtatggagttgagccagagaattacag8640

catgattaggtttgtagacccgctaactggacacacaatggatgaacaccctagagttga8700

catcagaatggttcaacaagagtttgaggagataaggaaagacatgattggggagggtga8760

actggatcgccaaagagtctaccacaatcctggtctacaggcttattttattgggaagaa8820

tacagaggaagcactcaaggttgatctcacaccacacagacctacacttctttgccaaaa8880

tagcaatgctatagcaggttttcctgagagggaggatgaactgcgtcagacaggattgcc8940

gcaagtagttcccaggttagatgtcccacgtgccaaagaaagagttgaaatggaaagcaa9000

gtctgtctataaaggactcagagattatagtggcatttctacattaatatgtcaactcac9060

aaattcatcagatggacacaaagaaacaatgtttggggttggctatggttccttcattat9120

cacaaatggacacttgtttagaaggaacaatggaatgcttacagttaagacatggcatgg9180

tgagtttgtgatacacaacactacacagctcaagatacattttatccaggggaaggatgt9240

gattttgattcgcatgccaaaggattttcctccattcggaaagcgcaacttcttcagaca9300

accaaagcgtgaggaacgggtttgcatggttgggacaaatttccaggaaaagagcttgcg9360

tgcaacagtttcagaatcttcaattatattacctgaagggaaaggctctttctggataca9420

ctggatcacaacccaagatggtttttgtggattacctcttgtctctgtgaatgatgggca9480

tattgttggtatacatgggttaacatctaatgattcagagaaaaatttcttcgttccact9540

cactgatgggtttgagaaggagtacctagagaatgctgataatttgtcgtgggataagca9600

ctggttttgggaaccaagcaagatagcatggggctctttgaacttagttgaggaacaacc9660

aaaagaggagttcaaaatatcaaagcttgtgtcggatctttttggaaacacagtgacagt9720

tcaagggagaaaagagagatgggttttggatgcaatggaaggcaatttagtggcttgtgg9780

gcaggctgacagtgcattggtaacaaagcatgtggttaaaggaaaatgcccctatttcgc9840

acaatatctttcagtgaatcaggaggcaaaatctttctttgagccactcatgggtgcgta9900

tcaaccaagccggttaaacaaagatgcattcaaacgagatttcttcaaatacaacaaacc9960

agttgttctgaatgaagttgatttccactcttttgagaaggcagtagctggagtgaaact10020

gatgatgatggaatttgattttaaagaatgcgtgtatgtgactgatcctgatgacatata10080

tgactctttgaatatgaaagctgcagttggtgcacagtacaaagggaagaagcaagatta10140

tttctctggaatggacagttttgacaaggaacgcctgctttatctcagttgtgaaaggtt10200

gttctatggagaaaaaggagtgtggaatggatccctgaaagcagagttgaggccaattga10260

aaaagtgcaagcaaacaaaactagaacattcacagcagcaccaattgacacactacttgg10320

agccaaggtttgtgttgatgatttcaacaatcagttctacagcctcaaccttacatgtcc10380

atggacagttggtatgaccaaattttacagaggttgggacaagttgatgagaagtttacc10440

tgatggatgggtttattgtcatgcagatggctcacaatttgatagttccttgacaccctt10500

attactgaacgcagttcttgatgttaggagcttcttcatggaagactggtgggtcggaag10560

agaaatgcttgaaaatctctatgctgaaatagtctacacaccaatcttagcacctgacgg10620

cacaatttttaaaaagttcagaggaaacaacagtgggcaaccatccacagttgtggacaa10680

taccttgatggtggtcattgccatgtactactcgtgttgtaagcaagggtggtcagatga10740

ggacatccaagaaaggttggtgtttttcgccaatggtgatgatattatcctggcagttag10800

tgatagagatacatggttatatgacactcttagcatttcgttcgctgagcttggcctcaa10860

ctacaactttgatgaacggacaaagagaagagaggagttatggttcatgtcacacaaagc10920

catgttagttgatgggatctacattccaaaacttgaacctgagagaattgtctctattct10980

agagtgggacaggagcaaagagcttatgcatcgcactgaggcgatatgtgcagcaatgat11040

tgaggcatggggatacactgagttgctacaagagattcgcaagttctatttgtggctctt11100

aagcaaggatgaatttaaggagctcgcttcgtctggaaaagcaccatatattgcagagac11160

agctttgagaaagctgtatacagatgttaatgcgcagacaagtgagctacaaagatatct11220

tgaggtactggactttaaccatgctgatgattgttgtgaatcagtgtccttacaatcagg11280

caaagagaaggaaggagacatggatgcaggcaaagatccaaagaagaacgccagcagtag11340

caaaggagctggtgcgagcagtaaggacgtgaatgttgggtcaaaagggaaggtggttcc11400

gcgtttgcagaagattacaaggaagatgaatcttccaatggttgaagggaagattatcct11460

cagtttagaccacttgcttgagtataaacctaatcaagttgatttattcaatactcgggc11520

aacaagaacacagtttgaggcatggtacaatgcagtcaaagatgaatatgaacttgatga11580

tgagcagatgggtgtcgtcatgaatggttttatggtatggtgcattgataatggcacgtc11640

tccagatgccaatggcgtgtgggtaatgatggatggagaggagcagattgaatacccact11700

gaagcccatcgttgaaaatgcaaaaccaactctgaggcaaatcatgcaccatttctcaga11760

tgcagcagaagcttacattgagatgagaaattctgaaagtccatatatgcctaggtatgg11820

actactgaggaatttgagagatagggagttagcccgttatgccttcgatttctatgaggt11880

cacctccaaaacaccgaacagggcaagagaggcaatagcacaaatgaaggctgcagctct11940

ctcgggagttaacaacaagctgtttgggcttgatggaaacatctcgaccaactccgaaaa12000

tactgaaaggcacactgcaagagatgtgaatcaaaacatgcattctcttttgggcatggg12060

cccacagcagtaaaggctaggtaaactggccacagttatcatttcgggtcgctttatagt12120

ttactatagtatagtagttgcacttcctttaagtatagtgtgattgcatcacctaataat12180

acttttgtttagagtggtttaaccaccttagtgtgctttatattatagtttatgaatggc12240

agggagaaccattgcaatgccggagcccttttcaagagtgattctatcatgcatagtggc12300

cgaggtacggcaatgtttgttgtcctaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa12360

aaaaaaaaaaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacc12420

tgggcatccgaaggaggacgcacgtccactcggatggctaagggagagccactgggtcag12480

ggagagctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaa12540

tcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgt12600

aataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtccc12660

gcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaatt12720

atcgcgcgcggtgtcatctatgttactagatcggaattcagattgtcgtttcccgccttc12780

agtttaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcg12840

tttattagaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttg12900

tatgtgcatgccaaccacaggagatctcagtgccacctgggtccttttcatcacgtgcta12960

taaaaataattataatttaaattttttaatataaatatataaattaaaaatagaaagtaa13020

aaaaagaaattaaagaaaaaatagtttttgttttccgaagatgtaaaagactctaggggg13080

atcgccaacaaatactaccttttatcttgctcttcctgctctcaggtattaatgccgaat13140

tgtttcatcttgtctgtgtagaagaccacacacgaaaatcctgtgattttacattttact13200

tatcgttaatcgaatgtatatctatttaatctgcttttcttgtctaataaatatatatgt13260

aaagtacgctttttgttgaaattttttaaacctttgtttatttttttttcttcattccgt13320

aactcttctaccttctttatttactttctaaaatccaaatacaaaacataaaaataaata13380

aacacagagtaaattcccaaattattccatcattaaaagatacgaggcgcgtgtaagtta13440

caggcaagcgatccgtctaagaaaccattattatcatgacattaacctataaaaataggc13500

gtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacaca13560

tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagccc13620

gtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcag13680

agcagattgtactgagagtgcaccatagatcacgacattactatatatataatataggaa13740

gcatttaatagaacagcatcgtaatatatgtgtactttgcagttatgacgccagatggca13800

gtagtggaagatattctttattgaaaaatagcttgtcaccttacgtacaatcttgatccg13860

gagcttttctttttttgccgattaagaattaattcggtcgaaaaaagaaaaggagagggc13920

caagagggagggcattggtgactattgagcacgtgagtatacgtgattaagcacacaaag13980

gcagcttggagtatgtctgttattaatttcacaggtagttctggtccattggtgaaagtt14040

tgcggcttgcagagcacagaggccgcagaatgtgctctagattccgatgctgacttgctg14100

ggtattatatgtgtgcccaatagaaagagaacaattgacccggttattgcaaggaaaatt14160

tcaagtcttgtaaaagcatataaaaatagttcaggcactccgaaatacttggttggcgtg14220

tttcgtaatcaacctaaggaggatgttttggctctggtcaatgattacggcattgatatc14280

gtccaactgcatggagatgagtcgtggcaagaataccaagagttcctcggtttgccagtt14340

attaaaagactcgtatttccaaaagactgcaacatactactcagtgcagcttcacagaaa14400

cctcattcgtttattcccttgtttgattcagaagcaggtgggacaggtgaacttttggat14460

tggaactcgatttctgactgggttggaaggcaagagagccccgaaagcttacattttatg14520

ttagctggtggactgacgccagaaaatgttggtgatgcgcttagattaaatggcgttatt14580

ggtgttgatgtaagcggaggtgtggagacaaatggtgtaaaagactctaacaaaatagca14640

aatttcgtcaaaaatgctaagaaataggttattactgagtagtatttatttaagtattgt14700

ttgtgcacttgccgatctatgcggtgtgaaataccgcacagatgcgtaaggagaaaatac14760

cgcatcaggaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaa14820

tcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaat14880

agaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacg14940

tggactccaacgtcaaagggcgaaaaaccgtctgtaaagcgctggctgaacccccagccg15000

gaactgaccccacaaggccctagcgtttgcaatgcaccaggtcatcattgacccaggcgt15060

gttccaccaggccgctgcctcgcaactcttcgcaggcttcgccgacctgctcgcgccact15120

tcttcacgcgggtggaatccgatccgcacatgaggcggaaggtttccagcttgagcgggt15180

acggctcccggtgcgagctgaaatagtcgaacatccgtcgggccgtcggcgacagcttgc15240

ggtacttctcccatatgaatttcgtgtagtggtcgccagcaaacagcacgacgatttcct15300

cgtcgatcaggacctggcaacgggacgttttcttgccacggtccaggacgcggaagcggt15360

gcagcagcgacaccgattccaggtgcccaacgcggtcggacgtgaagcccatcgccgtcg15420

cctgtaggcgcgacaggcattcctcggccttcgtgtaataccggccattgatcgaccagc15480

ccaggtcctggcaaagctcgtagaacgtgaaggtgatcggctcgccgataggggtgcgct15540

tcgcgtactccaacacctgctgccacaccagttcgtcatcgtcggcccgcagctcgacgc15600

cggtgtaggtgatcttcacgtccttgttgacgtggaaaatgaccttgttttgcagcgcct15660

cgcgcgggattttcttgttgcgcgtggtgaacagggcagagcgggccgtgtcgtttggca15720

tcgctcgcatcgtgtccggccacggcgcaatatcgaacaaggaaagctgcatttccttga15780

tctgctgcttcgtgtgtttcagcaacgcggcctgcttggcctcgctgacctgttttgcca15840

ggtcctcgccggcggtttttcgcttcttggtcgtcatagttcctcgcgtgtcgatggtca15900

tcgacttcgccaaacctgccgcctcctgttcgagacgacgcgaacgctccacggcggccg15960

atggcgcgggcagggcagggggagccagttgcacgctgtcgcgctcgatcttggccgtag16020

cttgctgggccatcgagccgacggactggaaggtttcgcggggcgcacgcatgacggtgc16080

ggcttgcgatggtttcggcatcctcggcggaaaaccccgcgtcgatcagttcttgcctgt16140

atgccttccggtcaaacgtccgattcattcaccctccttgcgggattgccccgactcacg16200

ccggggcaatgtgcccttattcctgatttgacccgcctggtgccttggtgtccagataat16260

ccaccttatcggcaatgaagtcggtcccgtagacc16295

<210>2

<211>8406

<212>dna

<213>大豆花叶病毒(soybeanmosaicvirus)

<400>2

atggcaacaatcatgattggaagcatggcgatctctgtgccaaacactcacgtcttgtgc60

gcatcgaattctgtgatgccggttcaagcagttcagatggcaaaacaagtgccttccgct120

cgaggggtgttatacacacttaaaagagagggcaacacgcaagtgcataagcacgaggag180

gcgttgcgcaaatttcaagaagcattcgaccaagatgttgacattcagcgaaggcttttg240

gtaaacaagcatagttccatacaatccacaaagaagaatggtttaaccttgcgtcgctta300

actttagagcaggctcgagcaaaagaagcggcaattgcaaggcgaaggcaagaagaggaa360

gactttctcaatgggaaatatgaacagcaattttatgctggtgtttccgctacaaagtcc420

atgaaatctgaaggagggagtgttggattcagaacaaaatactggagaccaactccaaag480

aagactaaagaaaggcgtgcaacatcgcagtgtaggaaaccaacatatgttttggaggag540

gttctttccatagcttcaaggagtggtaagctggttgaatttatcacaggaggcaaagga600

aagagagtcaaaatctgttatgtgcgcaagcatggcgcaatattgcccaagttctccctc660

ccgcatgaagaaggcaaatatatccatcaggagcttcagtatgcaagcacatatgagttt720

cttccttatatttgcatgtttgcaaaatataagagcataaatgcggatgatataacttat780

ggagatagtggtttactgtttgatgagcgatcatctttaaccacaaatcatactaagtta840

ccgtactttgtcgttcggggaaggaagaatgggaagcttgttaacgctcttgaagtggtt900

gaaaacatggatgatattcagcactactcccaaaatcctgaagctcagtttttccgtggt960

tggaaaaaggtgtttgataaaatgcctcctcatgtggagaatcatgaatgcaccattgat1020

ttcacaaatgagcaatgtggtgaattggcagcggctataagccaatcaatttttccagtt1080

aagaaactatcatgtaagcaatgtcggcagcacattaagcacctcagttgggaagagtat1140

aaacaattcctcctggctcatatgggctgccatgggactgaatggaaaactttccaagaa1200

attgatggcatgaagtatgtaaagagagtgattgagacatcaactgcggaaaacgcaagt1260

ctgcagacatcactggagattgtgcgtttaacgcagaactataagagcactcacatgctt1320

caaatacaggacattaataaggccctgatgaagggtccatcggtaacacagagcgagctg1380

gagcaagcttccaagcagctgctcgcaatgacacaatggtggaagaatcacatggctttg1440

actgatgaagatgcacttaaagtgttcaggaataagagatcttccaaagcactactcaac1500

ccaagtttactttgtgataaccagttggacaagaatggtaattttgtttggggagagcgt1560

ggcaggcattcaaagcgattctttgcgaattattttgaagaggtggttccttctgaaggg1620

tatagtaagtatgtgatcagaaagaatccaaacggacaaagggagttggcaattgggtca1680

cttattgtaccgttgaatttcgagcgcgctcgaatggcattacagggcaagagcgtacca1740

agaaagccaattacactgtcatgtatctcaagacaagacggaaactttgtatatccttgt1800

tgttgtgtcacacatgatgatggaaaagctttctattctgagctcaagagtcctacaaag1860

cgccatttggttattggaacgtcaggtgacccgaagtacattgatctaccagccactgat1920

gcagacaggatgtacatagctaaagaaggattttgttatcttaacatcttcttggcaatg1980

ttggttaatgtaaatgaagatgaggccaaggacttcacaaagatggtgagggatgttatt2040

ataccacagttaggaaagtggccgacaatgttagatgtagcaacagctgcatacatgcta2100

acagtttttcaccctgagaccaggaatgccgagctcccacgtattttagttgaccatgcg2160

tgtcaaaccatgcatgtgattgactcttttggatccttgacagttgggtaccatgttctt2220

aaagctggtacagtgaatcaattaattcaatttgcttctaatgaccttcagagtgagatg2280

aaattctacagagttggtggtgaagtgcaacagagaatgaagtgtgaaacagcacttata2340

acaagcattttcaaacctaagagaatggttcaaatccttgaaaatgacccatacattctc2400

ttgatgggcttggtttcaccttctatcttgattcacatgtatcgtatgaagcattttgag2460

aaaggggtggagttgtggataagtaaagaacatagtgtggcaaagattttcatcatattg2520

gaacaactcactaagagggtcgctgcaaatgatgtgctacttgagcaacttgaaatgatt2580

tcagaaacttctgagaggttcatgagtatcttagaggattgccctcaagcaccacattca2640

tacaagacggcaaaagatttgttgacaatgtacatagaaaggaaagcatccaatagccaa2700

ttggtggagaatggttttgtagatatgaatgacaaattgtacatggcatatgaaaaaatc2760

tactcagatcgcttgaagcaggaatggcgcgcattaagctggttggaaaaattttctaca2820

acatggcaattgaaaagatttgctccacatacggagaaatgtttgacaaagaaagttgta2880

gaagaaagcagcgcatcttcaggaaactttgcgagtgtgtgcttcatgaatgcccagtca2940

cacctaagaaatgtaagaaatacactttttcaaagatgtgaccaggtttggacagcatcg3000

gtgcgagcctttgtgaggttcataatttcaacacttcacagatgctacagtgatatagtt3060

tatctggtgaacatctgtataatcttttcattgctcgtccaaatgactagtgtgcttcag3120

aacattgtcaacacagcaaggagagacaaagcactcttgaatggatggaaaaggaaagaa3180

gatgaggaggccgtgattcatttgtatgaaatgtgtgaaaagatggaaggtggacatccg3240

agtattgagaaatttttggaccatgtcaagggagtcagacccgatctactccctatagca3300

gtgagcatgacaggacaatcagaggatgtctccgcacaggccaaaacagtaactcaatta3360

caacttgagaaaatcgtggcattcatggctttgttgaccatgtgtattgataatgaaagg3420

agtgatgcggttttcaaagtattgagtaagttgaaggcatttttcagcacaatgggtgag3480

gatgttaaagtgcagagtctcgatgagattcaaaatattgatgaagataaaaagctcaca3540

attgatttcgaccttgaaacgaataaggagtcttccagtgtctcttttgatgtcaaattt3600

gaagcctggtggaatagacagttggaacagaatagagtaattccacactacaggtcgaca3660

ggtgagtttctggaattcacaagagaaacagcagccaagattgcaaatttggtagctact3720

tcaagccacacagaatttttgattagaggtgcagttggctcagggaaatcaacaggttta3780

ccacaccacctttcaaagaagggcaaagttctgctactggagccaactagaccattagca3840

gaaaatgtcagtaagcagttgagccttgaacctttctatcacaatgtaacactgagaatg3900

agagggctgagtaagtttggttcaagcaacatagttgtcatgacaagtgggtttgcgttt3960

cattactatgttaacaatccacaacagttatctgatttcgattttatcataatagatgaa4020

tgccatgtgcaagatagcccaacgattgcattcaactgtgcgcttaaagagtttgaattc4080

agtggcaagcttataaaagtgtctgcaacacctccagggagagagtgcgagttcacaacg4140

cagcatccagtgaagttgaaggttgaagaccacttatcttttcagaactttgtgcaagct4200

caaggtacaggatcaaatgctgatatgatccaacatgggaacaacttacttgtatatgtt4260

gcaagctacaatgaagttgaccaattgtcacgattattaactgaaaaacattacaaggtg4320

acaaaggttgatgggagaacaatgcaaatgggaaatgtagagattgcaaccacaggcacg4380

gaaggaaaaccacacttcatagtcgcaacaaacatcattgagaatggagtgactcttgat4440

attgattgtgtaattgattttggacttaaagtggtggctactcttgatacagataaccgg4500

tgtgtgcgttacaacaaacagtcagtttcttatggagagcgaattcagagacttggcaga4560

gttggtcgttgtaaacctgggtttgcgcttaggattggacacacgggaaaaggagttgag4620

gaagttcccgagttcatagctacagaggcagcctttctatcctttgcttatgggttgcca4680

gttacaacacagagtgtctcgaccaatatactgtcccgttgcacagtaaaacaagctcga4740

gtggctttgaattttgagctaactccattcttcaccaccaacttcataaagtatgatggt4800

agcatgcacccagaaattcacagattgctcaagtcctataaactcagggagtctgagatg4860

ttgttgaccaagctagccataccatatcagtttgttgggcagtggataacagtcaaggaa4920

tatgaacgtcaaggtgtccaccttaattgcccagagaaagtgaaaatacctttctatgtg4980

catggaataccagacaagttgtatgagatgttgtgggacacagtttgtaaatacaagaat5040

gatgctggattcggctcaattaagagtgtgaatgcaacgaagattagttacactctaagc5100

actgatccgacagcaattcctcgcacacttgcaatactggaccatttactgagcgaggaa5160

atgactaagaagagtcattttgacacaattggctctgctgtcactgggcattccttttct5220

cttgcaggtatagctgacggaattagaaagaggtacttgaaggactacacacagcacaat5280

atagccgtcttacaacaggctaaagcacagctgctagaatttgactgcaacaaagttgac5340

atcaacaacctgcacaatgttgagggtataggcattttaaatgcagttcaactacagagc5400

aagcaagaggtgagcaaatttttgcagcttaaaggaaagtgggatggaaagaaattcatg5460

aatgatgctgtagtggctatcttcactttagtgggcggtggttggatgttatgggattac5520

ctcacaagagttatacgtgaaccagtatcaactcaaggaaagaagaggcaaatacaaaag5580

ctcaaattcagggatgcctttgacagaaaagtaggccgtgagatatacgcggatgattac5640

accatggagcatacctttggggaggcatacaccaagaaaggaaagcagaaaggtagcact5700

cgtacaaaagggatgggtcgcaagtcgaggaacttcatacatctgtatggagttgagcca5760

gagaattacagcatgattaggtttgtagacccgctaactggacacacaatggatgaacac5820

cctagagttgacatcagaatggttcaacaagagtttgaggagataaggaaagacatgatt5880

ggggagggtgaactggatcgccaaagagtctaccacaatcctggtctacaggcttatttt5940

attgggaagaatacagaggaagcactcaaggttgatctcacaccacacagacctacactt6000

ctttgccaaaatagcaatgctatagcaggttttcctgagagggaggatgaactgcgtcag6060

acaggattgccgcaagtagttcccaggttagatgtcccacgtgccaaagaaagagttgaa6120

atggaaagcaagtctgtctataaaggactcagagattatagtggcatttctacattaata6180

tgtcaactcacaaattcatcagatggacacaaagaaacaatgtttggggttggctatggt6240

tccttcattatcacaaatggacacttgtttagaaggaacaatggaatgcttacagttaag6300

acatggcatggtgagtttgtgatacacaacactacacagctcaagatacattttatccag6360

gggaaggatgtgattttgattcgcatgccaaaggattttcctccattcggaaagcgcaac6420

ttcttcagacaaccaaagcgtgaggaacgggtttgcatggttgggacaaatttccaggaa6480

aagagcttgcgtgcaacagtttcagaatcttcaattatattacctgaagggaaaggctct6540

ttctggatacactggatcacaacccaagatggtttttgtggattacctcttgtctctgtg6600

aatgatgggcatattgttggtatacatgggttaacatctaatgattcagagaaaaatttc6660

ttcgttccactcactgatgggtttgagaaggagtacctagagaatgctgataatttgtcg6720

tgggataagcactggttttgggaaccaagcaagatagcatggggctctttgaacttagtt6780

gaggaacaaccaaaagaggagttcaaaatatcaaagcttgtgtcggatctttttggaaac6840

acagtgacagttcaagggagaaaagagagatgggttttggatgcaatggaaggcaattta6900

gtggcttgtgggcaggctgacagtgcattggtaacaaagcatgtggttaaaggaaaatgc6960

ccctatttcgcacaatatctttcagtgaatcaggaggcaaaatctttctttgagccactc7020

atgggtgcgtatcaaccaagccggttaaacaaagatgcattcaaacgagatttcttcaaa7080

tacaacaaaccagttgttctgaatgaagttgatttccactcttttgagaaggcagtagct7140

ggagtgaaactgatgatgatggaatttgattttaaagaatgcgtgtatgtgactgatcct7200

gatgacatatatgactctttgaatatgaaagctgcagttggtgcacagtacaaagggaag7260

aagcaagattatttctctggaatggacagttttgacaaggaacgcctgctttatctcagt7320

tgtgaaaggttgttctatggagaaaaaggagtgtggaatggatccctgaaagcagagttg7380

aggccaattgaaaaagtgcaagcaaacaaaactagaacattcacagcagcaccaattgac7440

acactacttggagccaaggtttgtgttgatgatttcaacaatcagttctacagcctcaac7500

cttacatgtccatggacagttggtatgaccaaattttacagaggttgggacaagttgatg7560

agaagtttacctgatggatgggtttattgtcatgcagatggctcacaatttgatagttcc7620

ttgacacccttattactgaacgcagttcttgatgttaggagcttcttcatggaagactgg7680

tgggtcggaagagaaatgcttgaaaatctctatgctgaaatagtctacacaccaatctta7740

gcacctgacggcacaatttttaaaaagttcagaggaaacaacagtgggcaaccatccaca7800

gttgtggacaataccttgatggtggtcattgccatgtactactcgtgttgtaagcaaggg7860

tggtcagatgaggacatccaagaaaggttggtgtttttcgccaatggtgatgatattatc7920

ctggcagttagtgatagagatacatggttatatgacactcttagcatttcgttcgctgag7980

cttggcctcaactacaactttgatgaacggacaaagagaagagaggagttatggttcatg8040

tcacacaaagccatgttagttgatgggatctacattccaaaacttgaacctgagagaatt8100

gtctctattctagagtgggacaggagcaaagagcttatgcatcgcactgaggcgatatgt8160

gcagcaatgattgaggcatggggatacactgagttgctacaagagattcgcaagttctat8220

ttgtggctcttaagcaaggatgaatttaaggagctcgcttcgtctggaaaagcaccatat8280

attgcagagacagctttgagaaagctgtatacagatgttaatgcgcagacaagtgagcta8340

caaagatatcttgaggtactggactttaaccatgctgatgattgttgtgaatcagtgtcc8400

ttacaa8406

<210>3

<211>14217

<212>dna

<213>人工序列(artificialsequence)

<400>3

ccaaatacttgccgtgggcctcggcctgagagccaaaacacttgatgcggaagaagtcgg60

tgcgctcctgcttgtcgccggcatcgttgcgccacatctaggtactaaaacaattcatcc120

agtaaaatataatattttattttctcccaatcaggcttgatccccagtaagtcaaaaaat180

agctcgacatactgttcttccccgatatcctccctgatcgaccggacgcagaaggcaatg240

tcataccacttgtccgccctgccgcttctcccaagatcaataaagccacttactttgcca300

tctttcacaaagatgttgctgtctcccaggtcgccgtgggaaaagacaagttcctcttcg360

ggcttttccgtctttaaaaaatcatacagctcgcgcggatctttaaatggggtgtcttct420

tcccagttttcgcaatccacatcggccagatcgttattcagtaagtaatccaattcggct480

aagcggctgtctaagctattcgtatagggacaatccgatatgtcgatggagtgaaagagc540

ctgatgcactccgcatacagctcgataatcttttcagggctttgttcatcttcatactct600

tccgagcaaaggacgccatcggcctcactcatgagcagattgctccagccatcatgccgt660

tcaaggtgcaggacctttggaacaggcagctttccttccagccatagcatcatgtccttt720

tcccgttccacatcataggtggtccctttataccggctgtccgtcatttttaaatatagg780

ttttcattttctcccaccagcttatataccttagcaggagacattccttccgtatctttt840

acgcagcggtatttttcgatcagttttttcaattccggtgatattctcattttagccatt900

tattatttccttcctcttttctacagtatttaaagataccccaagaagctaattataaca960

agacgaactccaattcactgttccttgcattctaaaactttaaataccagaaaacagctt1020

tttcaaagttgttttcaaagttggcgtataacatagtatcgacggagccgattttgaaac1080

cacaattatgggtgatgctgcaactcgagcgggccgggagggttcgagaagggggggcac1140

cccccttcggcgtgcgcggtcacgcgcacagggcgcagccctggttaaaaacaaggttta1200

taaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaaaaacgggcgga1260

aacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcg1320

cccctcatctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcatctgtcag1380

tagtcgcgcccctcaagtgtcaataccgcagggcacttatccccaggcttgtccacatca1440

tctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgaggctggccagctcc1500

acgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggtgagtcgg1560

cccctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggt1620

atccacaacgccggcggccgcggtgtctcgcacacggcttcgacggcgtttctggcgcgt1680

ttgcagggccatagacggccgccagcccagcggcgagggcaaccagcccggtgagcgtct1740

ctagtggactgatgggctgcctgtatcgagtggtgattttgtgccgagctgccggtcggg1800

gagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacgcttagaca1860

acttaataacacgggggactctaatcaaacaagtttgtacaaaaaagctgaacgagaaac1920

gtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacata1980

atactgtaaaacacaacatatccagtcactatggcggcccctcgaggggccgcattaggc2040

accccaggctttacatggcaacaatcatgattggaagcatggcgatctctgtgccaaaca2100

ctcacgtcttgtgcgcatcgaattctgtgatgccggttcaagcagttcagatggcaaaac2160

aagtgccttccgctcgaggggtgttatacacacttaaaagagagggcaacacgcaagtgc2220

ataagcacgaggaggcgttgcgcaaatttcaagaagcattcgaccaagatgttgacattc2280

agcgaaggcttttggtaaacaagcatagttccatacaatccacaaagaagaatggtttaa2340

ccttgcgtcgcttaactttagagcaggctcgagcaaaagaagcggcaattgcaaggcgaa2400

ggcaagaagaggaagactttctcaatgggaaatatgaacagcaattttatgctggtgttt2460

ccgctacaaagtccatgaaatctgaaggagggagtgttggattcagaacaaaatactgga2520

gaccaactccaaagaagactaaagaaaggcgtgcaacatcgcagtgtaggaaaccaacat2580

atgttttggaggaggttctttccatagcttcaaggagtggtaagctggttgaatttatca2640

caggaggcaaaggaaagagagtcaaaatctgttatgtgcgcaagcatggcgcaatattgc2700

ccaagttctccctcccgcatgaagaaggcaaatatatccatcaggagcttcagtatgcaa2760

gcacatatgagtttcttccttatatttgcatgtttgcaaaatataagagcataaatgcgg2820

atgatataacttatggagatagtggtttactgtttgatgagcgatcatctttaaccacaa2880

atcatactaagttaccgtactttgtcgttcggggaaggaagaatgggaagcttgttaacg2940

ctcttgaagtggttgaaaacatggatgatattcagcactactcccaaaatcctgaagctc3000

agtttttccgtggttggaaaaaggtgtttgataaaatgcctcctcatgtggagaatcatg3060

aatgcaccattgatttcacaaatgagcaatgtggtgaattggcagcggctataagccaat3120

caatttttccagttaagaaactatcatgtaagcaatgtcggcagcacattaagcacctca3180

gttgggaagagtataaacaattcctcctggctcatatgggctgccatgggactgaatgga3240

aaactttccaagaaattgatggcatgaagtatgtaaagagagtgattgagacatcaactg3300

cggaaaacgcaagtctgcagacatcactggagattgtgcgtttaacgcagaactataaga3360

gcactcacatgcttcaaatacaggacattaataaggccctgatgaagggtccatcggtaa3420

cacagagcgagctggagcaagcttccaagcagctgctcgcaatgacacaatggtggaaga3480

atcacatggctttgactgatgaagatgcacttaaagtgttcaggaataagagatcttcca3540

aagcactactcaacccaagtttactttgtgataaccagttggacaagaatggtaattttg3600

tttggggagagcgtggcaggcattcaaagcgattctttgcgaattattttgaagaggtgg3660

ttccttctgaagggtatagtaagtatgtgatcagaaagaatccaaacggacaaagggagt3720

tggcaattgggtcacttattgtaccgttgaatttcgagcgcgctcgaatggcattacagg3780

gcaagagcgtaccaagaaagccaattacactgtcatgtatctcaagacaagacggaaact3840

ttgtatatccttgttgttgtgtcacacatgatgatggaaaagctttctattctgagctca3900

agagtcctacaaagcgccatttggttattggaacgtcaggtgacccgaagtacattgatc3960

taccagccactgatgcagacaggatgtacatagctaaagaaggattttgttatcttaaca4020

tcttcttggcaatgttggttaatgtaaatgaagatgaggccaaggacttcacaaagatgg4080

tgagggatgttattataccacagttaggaaagtggccgacaatgttagatgtagcaacag4140

ctgcatacatgctaacagtttttcaccctgagaccaggaatgccgagctcccacgtattt4200

tagttgaccatgcgtgtcaaaccatgcatgtgattgactcttttggatccttgacagttg4260

ggtaccatgttcttaaagctggtacagtgaatcaattaattcaatttgcttctaatgacc4320

ttcagagtgagatgaaattctacagagttggtggtgaagtgcaacagagaatgaagtgtg4380

aaacagcacttataacaagcattttcaaacctaagagaatggttcaaatccttgaaaatg4440

acccatacattctcttgatgggcttggtttcaccttctatcttgattcacatgtatcgta4500

tgaagcattttgagaaaggggtggagttgtggataagtaaagaacatagtgtggcaaaga4560

ttttcatcatattggaacaactcactaagagggtcgctgcaaatgatgtgctacttgagc4620

aacttgaaatgatttcagaaacttctgagaggttcatgagtatcttagaggattgccctc4680

aagcaccacattcatacaagacggcaaaagatttgttgacaatgtacatagaaaggaaag4740

catccaatagccaattggtggagaatggttttgtagatatgaatgacaaattgtacatgg4800

catatgaaaaaatctactcagatcgcttgaagcaggaatggcgcgcattaagctggttgg4860

aaaaattttctacaacatggcaattgaaaagatttgctccacatacggagaaatgtttga4920

caaagaaagttgtagaagaaagcagcgcatcttcaggaaactttgcgagtgtgtgcttca4980

tgaatgcccagtcacacctaagaaatgtaagaaatacactttttcaaagatgtgaccagg5040

tttggacagcatcggtgcgagcctttgtgaggttcataatttcaacacttcacagatgct5100

acagtgatatagtttatctggtgaacatctgtataatcttttcattgctcgtccaaatga5160

ctagtgtgcttcagaacattgtcaacacagcaaggagagacaaagcactcttgaatggat5220

ggaaaaggaaagaagatgaggaggccgtgattcatttgtatgaaatgtgtgaaaagatgg5280

aaggtggacatccgagtattgagaaatttttggaccatgtcaagggagtcagacccgatc5340

tactccctatagcagtgagcatgacaggacaatcagaggatgtctccgcacaggccaaaa5400

cagtaactcaattacaacttgagaaaatcgtggcattcatggctttgttgaccatgtgta5460

ttgataatgaaaggagtgatgcggttttcaaagtattgagtaagttgaaggcatttttca5520

gcacaatgggtgaggatgttaaagtgcagagtctcgatgagattcaaaatattgatgaag5580

ataaaaagctcacaattgatttcgaccttgaaacgaataaggagtcttccagtgtctctt5640

ttgatgtcaaatttgaagcctggtggaatagacagttggaacagaatagagtaattccac5700

actacaggtcgacaggtgagtttctggaattcacaagagaaacagcagccaagattgcaa5760

atttggtagctacttcaagccacacagaatttttgattagaggtgcagttggctcaggga5820

aatcaacaggtttaccacaccacctttcaaagaagggcaaagttctgctactggagccaa5880

ctagaccattagcagaaaatgtcagtaagcagttgagccttgaacctttctatcacaatg5940

taacactgagaatgagagggctgagtaagtttggttcaagcaacatagttgtcatgacaa6000

gtgggtttgcgtttcattactatgttaacaatccacaacagttatctgatttcgatttta6060

tcataatagatgaatgccatgtgcaagatagcccaacgattgcattcaactgtgcgctta6120

aagagtttgaattcagtggcaagcttataaaagtgtctgcaacacctccagggagagagt6180

gcgagttcacaacgcagcatccagtgaagttgaaggttgaagaccacttatcttttcaga6240

actttgtgcaagctcaaggtacaggatcaaatgctgatatgatccaacatgggaacaact6300

tacttgtatatgttgcaagctacaatgaagttgaccaattgtcacgattattaactgaaa6360

aacattacaaggtgacaaaggttgatgggagaacaatgcaaatgggaaatgtagagattg6420

caaccacaggcacggaaggaaaaccacacttcatagtcgcaacaaacatcattgagaatg6480

gagtgactcttgatattgattgtgtaattgattttggacttaaagtggtggctactcttg6540

atacagataaccggtgtgtgcgttacaacaaacagtcagtttcttatggagagcgaattc6600

agagacttggcagagttggtcgttgtaaacctgggtttgcgcttaggattggacacacgg6660

gaaaaggagttgaggaagttcccgagttcatagctacagaggcagcctttctatcctttg6720

cttatgggttgccagttacaacacagagtgtctcgaccaatatactgtcccgttgcacag6780

taaaacaagctcgagtggctttgaattttgagctaactccattcttcaccaccaacttca6840

taaagtatgatggtagcatgcacccagaaattcacagattgctcaagtcctataaactca6900

gggagtctgagatgttgttgaccaagctagccataccatatcagtttgttgggcagtgga6960

taacagtcaaggaatatgaacgtcaaggtgtccaccttaattgcccagagaaagtgaaaa7020

tacctttctatgtgcatggaataccagacaagttgtatgagatgttgtgggacacagttt7080

gtaaatacaagaatgatgctggattcggctcaattaagagtgtgaatgcaacgaagatta7140

gttacactctaagcactgatccgacagcaattcctcgcacacttgcaatactggaccatt7200

tactgagcgaggaaatgactaagaagagtcattttgacacaattggctctgctgtcactg7260

ggcattccttttctcttgcaggtatagctgacggaattagaaagaggtacttgaaggact7320

acacacagcacaatatagccgtcttacaacaggctaaagcacagctgctagaatttgact7380

gcaacaaagttgacatcaacaacctgcacaatgttgagggtataggcattttaaatgcag7440

ttcaactacagagcaagcaagaggtgagcaaatttttgcagcttaaaggaaagtgggatg7500

gaaagaaattcatgaatgatgctgtagtggctatcttcactttagtgggcggtggttgga7560

tgttatgggattacctcacaagagttatacgtgaaccagtatcaactcaaggaaagaaga7620

ggcaaatacaaaagctcaaattcagggatgcctttgacagaaaagtaggccgtgagatat7680

acgcggatgattacaccatggagcatacctttggggaggcatacaccaagaaaggaaagc7740

agaaaggtagcactcgtacaaaagggatgggtcgcaagtcgaggaacttcatacatctgt7800

atggagttgagccagagaattacagcatgattaggtttgtagacccgctaactggacaca7860

caatggatgaacaccctagagttgacatcagaatggttcaacaagagtttgaggagataa7920

ggaaagacatgattggggagggtgaactggatcgccaaagagtctaccacaatcctggtc7980

tacaggcttattttattgggaagaatacagaggaagcactcaaggttgatctcacaccac8040

acagacctacacttctttgccaaaatagcaatgctatagcaggttttcctgagagggagg8100

atgaactgcgtcagacaggattgccgcaagtagttcccaggttagatgtcccacgtgcca8160

aagaaagagttgaaatggaaagcaagtctgtctataaaggactcagagattatagtggca8220

tttctacattaatatgtcaactcacaaattcatcagatggacacaaagaaacaatgtttg8280

gggttggctatggttccttcattatcacaaatggacacttgtttagaaggaacaatggaa8340

tgcttacagttaagacatggcatggtgagtttgtgatacacaacactacacagctcaaga8400

tacattttatccaggggaaggatgtgattttgattcgcatgccaaaggattttcctccat8460

tcggaaagcgcaacttcttcagacaaccaaagcgtgaggaacgggtttgcatggttggga8520

caaatttccaggaaaagagcttgcgtgcaacagtttcagaatcttcaattatattacctg8580

aagggaaaggctctttctggatacactggatcacaacccaagatggtttttgtggattac8640

ctcttgtctctgtgaatgatgggcatattgttggtatacatgggttaacatctaatgatt8700

cagagaaaaatttcttcgttccactcactgatgggtttgagaaggagtacctagagaatg8760

ctgataatttgtcgtgggataagcactggttttgggaaccaagcaagatagcatggggct8820

ctttgaacttagttgaggaacaaccaaaagaggagttcaaaatatcaaagcttgtgtcgg8880

atctttttggaaacacagtgacagttcaagggagaaaagagagatgggttttggatgcaa8940

tggaaggcaatttagtggcttgtgggcaggctgacagtgcattggtaacaaagcatgtgg9000

ttaaaggaaaatgcccctatttcgcacaatatctttcagtgaatcaggaggcaaaatctt9060

tctttgagccactcatgggtgcgtatcaaccaagccggttaaacaaagatgcattcaaac9120

gagatttcttcaaatacaacaaaccagttgttctgaatgaagttgatttccactcttttg9180

agaaggcagtagctggagtgaaactgatgatgatggaatttgattttaaagaatgcgtgt9240

atgtgactgatcctgatgacatatatgactctttgaatatgaaagctgcagttggtgcac9300

agtacaaagggaagaagcaagattatttctctggaatggacagttttgacaaggaacgcc9360

tgctttatctcagttgtgaaaggttgttctatggagaaaaaggagtgtggaatggatccc9420

tgaaagcagagttgaggccaattgaaaaagtgcaagcaaacaaaactagaacattcacag9480

cagcaccaattgacacactacttggagccaaggtttgtgttgatgatttcaacaatcagt9540

tctacagcctcaaccttacatgtccatggacagttggtatgaccaaattttacagaggtt9600

gggacaagttgatgagaagtttacctgatggatgggtttattgtcatgcagatggctcac9660

aatttgatagttccttgacacccttattactgaacgcagttcttgatgttaggagcttct9720

tcatggaagactggtgggtcggaagagaaatgcttgaaaatctctatgctgaaatagtct9780

acacaccaatcttagcacctgacggcacaatttttaaaaagttcagaggaaacaacagtg9840

ggcaaccatccacagttgtggacaataccttgatggtggtcattgccatgtactactcgt9900

gttgtaagcaagggtggtcagatgaggacatccaagaaaggttggtgtttttcgccaatg9960

gtgatgatattatcctggcagttagtgatagagatacatggttatatgacactcttagca10020

tttcgttcgctgagcttggcctcaactacaactttgatgaacggacaaagagaagagagg10080

agttatggttcatgtcacacaaagccatgttagttgatgggatctacattccaaaacttg10140

aacctgagagaattgtctctattctagagtgggacaggagcaaagagcttatgcatcgca10200

ctgaggcgatatgtgcagcaatgattgaggcatggggatacactgagttgctacaagaga10260

ttcgcaagttctatttgtggctcttaagcaaggatgaatttaaggagctcgcttcgtctg10320

gaaaagcaccatatattgcagagacagctttgagaaagctgtatacagatgttaatgcgc10380

agacaagtgagctacaaagatatcttgaggtactggactttaaccatgctgatgattgtt10440

gtgaatcagtgtccttacaacttatacacagccagtctgcaggtcgaccatagtgactgg10500

atatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatata10560

ttgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggttcgatct10620

agaggatccgccttcagtttaaactatcagtgtttgacaggatatattggcgggtaaacc10680

taagagaaaagagcgtttattagaataatcggatatttaaaagggcgtgaaaaggtttat10740

ccgttcgtccatttgtatgtgcatgccaaccacaggagatctcagtgccacctgggtcct10800

tttcatcacgtgctataaaaataattataatttaaattttttaatataaatatataaatt10860

aaaaatagaaagtaaaaaaagaaattaaagaaaaaatagtttttgttttccgaagatgta10920

aaagactctagggggatcgccaacaaatactaccttttatcttgctcttcctgctctcag10980

gtattaatgccgaattgtttcatcttgtctgtgtagaagaccacacacgaaaatcctgtg11040

attttacattttacttatcgttaatcgaatgtatatctatttaatctgcttttcttgtct11100

aataaatatatatgtaaagtacgctttttgttgaaattttttaaacctttgtttattttt11160

ttttcttcattccgtaactcttctaccttctttatttactttctaaaatccaaatacaaa11220

acataaaaataaataaacacagagtaaattcccaaattattccatcattaaaagatacga11280

ggcgcgtgtaagttacaggcaagcgatccgtctaagaaaccattattatcatgacattaa11340

cctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtg11400

aaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccg11460

ggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggctta11520

actatgcggcatcagagcagattgtactgagagtgcaccatagatcacgacattactata11580

tatataatataggaagcatttaatagaacagcatcgtaatatatgtgtactttgcagtta11640

tgacgccagatggcagtagtggaagatattctttattgaaaaatagcttgtcaccttacg11700

tacaatcttgatccggagcttttctttttttgccgattaagaattaattcggtcgaaaaa11760

agaaaaggagagggccaagagggagggcattggtgactattgagcacgtgagtatacgtg11820

attaagcacacaaaggcagcttggagtatgtctgttattaatttcacaggtagttctggt11880

ccattggtgaaagtttgcggcttgcagagcacagaggccgcagaatgtgctctagattcc11940

gatgctgacttgctgggtattatatgtgtgcccaatagaaagagaacaattgacccggtt12000

attgcaaggaaaatttcaagtcttgtaaaagcatataaaaatagttcaggcactccgaaa12060

tacttggttggcgtgtttcgtaatcaacctaaggaggatgttttggctctggtcaatgat12120

tacggcattgatatcgtccaactgcatggagatgagtcgtggcaagaataccaagagttc12180

ctcggtttgccagttattaaaagactcgtatttccaaaagactgcaacatactactcagt12240

gcagcttcacagaaacctcattcgtttattcccttgtttgattcagaagcaggtgggaca12300

ggtgaacttttggattggaactcgatttctgactgggttggaaggcaagagagccccgaa12360

agcttacattttatgttagctggtggactgacgccagaaaatgttggtgatgcgcttaga12420

ttaaatggcgttattggtgttgatgtaagcggaggtgtggagacaaatggtgtaaaagac12480

tctaacaaaatagcaaatttcgtcaaaaatgctaagaaataggttattactgagtagtat12540

ttatttaagtattgtttgtgcacttgccgatctatgcggtgtgaaataccgcacagatgc12600

gtaaggagaaaataccgcatcaggaaattgtaagcgttaatattttgttaaaattcgcgt12660

taaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatccctt12720

ataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtc12780

cactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctgtaaagcgctgg12840

ctgaacccccagccggaactgaccccacaaggccctagcgtttgcaatgcaccaggtcat12900

cattgacccaggcgtgttccaccaggccgctgcctcgcaactcttcgcaggcttcgccga12960

cctgctcgcgccacttcttcacgcgggtggaatccgatccgcacatgaggcggaaggttt13020

ccagcttgagcgggtacggctcccggtgcgagctgaaatagtcgaacatccgtcgggccg13080

tcggcgacagcttgcggtacttctcccatatgaatttcgtgtagtggtcgccagcaaaca13140

gcacgacgatttcctcgtcgatcaggacctggcaacgggacgttttcttgccacggtcca13200

ggacgcggaagcggtgcagcagcgacaccgattccaggtgcccaacgcggtcggacgtga13260

agcccatcgccgtcgcctgtaggcgcgacaggcattcctcggccttcgtgtaataccggc13320

cattgatcgaccagcccaggtcctggcaaagctcgtagaacgtgaaggtgatcggctcgc13380

cgataggggtgcgcttcgcgtactccaacacctgctgccacaccagttcgtcatcgtcgg13440

cccgcagctcgacgccggtgtaggtgatcttcacgtccttgttgacgtggaaaatgacct13500

tgttttgcagcgcctcgcgcgggattttcttgttgcgcgtggtgaacagggcagagcggg13560

ccgtgtcgtttggcatcgctcgcatcgtgtccggccacggcgcaatatcgaacaaggaaa13620

gctgcatttccttgatctgctgcttcgtgtgtttcagcaacgcggcctgcttggcctcgc13680

tgacctgttttgccaggtcctcgccggcggtttttcgcttcttggtcgtcatagttcctc13740

gcgtgtcgatggtcatcgacttcgccaaacctgccgcctcctgttcgagacgacgcgaac13800

gctccacggcggccgatggcgcgggcagggcagggggagccagttgcacgctgtcgcgct13860

cgatcttggccgtagcttgctgggccatcgagccgacggactggaaggtttcgcggggcg13920

cacgcatgacggtgcggcttgcgatggtttcggcatcctcggcggaaaaccccgcgtcga13980

tcagttcttgcctgtatgccttccggtcaaacgtccgattcattcaccctccttgcggga14040

ttgccccgactcacgccggggcaatgtgcccttattcctgatttgacccgcctggtgcct14100

tggtgtccagataatccaccttatcggcaatgaagtcggtcccgtagaccgtctggccgt14160

ccttctcgtacttggtattccgaatcttgccctgcacgaataccagcgaccccttgc14217