产生C6-C10脂肪酸衍生物的经遗传修饰的细胞的制作方法

这项工作由美国能源部根据合同号de-ee0070007支持。美国政府具有本发明的某些权利。本发明涉及产生c6-c10脂肪酸和脂肪酸衍生物如c6-c10脂肪酸酯和c6-c10脂肪醇的经遗传修饰的细胞。脂肪酸和脂肪酸衍生物可用作漆(lacquer)、油漆(paint)、清漆(varnish)和其它组合物的溶剂；作为有机树脂的增塑剂、作为芳香剂和调味剂、作为喷气式飞机(jet)和其它内燃机的燃料以及作为制造各种下游产品的原料。因此，脂肪酸和衍生物目前在工业上使用不可再生的矿物燃料作为碳源或者由源自植物如棕榈或椰子的油生成。对特定链长的选择性是这些方法中的重要缺点—产物倾向于是具有碳原子数范围的化合物的混合物。将化合物分离成离散的链长是困难的，并且通常将大部分原料转化为不具有所需碳原子数的较低价值产物。生物细胞天然产生脂肪酸衍生物。例如，几乎所有活细胞都产生脂肪酸的甘油三酯以及其它脂肪酸酯。甘油三酯和其它酯在细胞的代谢、细胞结构和其它生物过程中起重要作用，并且可执行其它有用的功能，如储存能量。生物过程潜在地提供在工业上生产脂肪酸衍生物的方式。除了其它潜在优点之外，在一些情况下以生物方式产生的脂肪酸和脂肪酸衍生物产生可依赖于每年可再生的碳源如糖，而不是矿物燃料。生物系统中产生的脂肪酸基团倾向于具有12个碳原子或更大的链长。因此，天然存在的细胞是c12和更高级脂肪酸和衍生物的良好来源。例如，可将由这些细胞天然产生的甘油三酯水解以产生c12或更高级脂肪酸，其进而可转化为其它衍生物，如酯或醇。在另一方面，很少细胞以大量天然产生c6-c10链长的脂肪酸。短链脂肪酸可由以生物方式产生的油和脂肪(和/或其组成脂肪酸)产生，但这样做需要用于必要的加工设备和操作成本的进一步资金成本以实现转化为较短链长。此外，由于天气对作物生产的影响，原料的成本每年或从一个地理位置到另一个地理位置会有很大差异。生物细胞通过天然代谢途径产生脂肪酸衍生物，所述天然代谢途径以乙酰辅酶a和丙二酰基-acp开始。乙酰辅酶a与丙二酰基-acp缩合，同时失去二氧化碳和辅酶a，以产生3-酮丁酰基-acp。随后的酶促反应将3-酮丁酰基-acp依次转化为3-羟基丁酰基-acp，然后转化为反式-2-丁烯酰基-acp(伴随失水)，并且最后转化为丁酰基-acp。丁酰基-acp可重新进入这种反应循环代替乙酰基-辅酶a以产生己酰基-acp。此循环自身重复，从而通过一次添加两个碳原子在每次重复中产生更长的碳原子链，直到通过一些其它细胞过程终止。然而，这种天然acp依赖性途径具有c6-c10链长的低终止率，从而导致c6-c10脂肪酸和脂肪酸衍生物的低产生。okamura等人,于pnas第107卷,第25期,第11265-11270页(2010)中报告由土壤分离的链霉菌属(streptomyces)菌株的npht7基因产生的酶催化乙酰基-辅酶a和丙二酰基-辅酶a的单一缩合以产生乙酰乙酰基-辅酶a。如us2014/0051136中进一步描述的，c4脂肪酸衍生物可经由辅酶a依赖性途径而不是天然acp依赖性途径、通过经修饰以包含npht7基因和编码3-酮酰基-辅酶a还原酶、3-羟基酰基-辅酶a脱水酶和反式-2-烯醇-辅酶a还原酶的其它非天然基因的细胞产生。然而，npht7基因对乙酰基-辅酶a和丙二酰基-辅酶a反应以产生c4脂肪酸和衍生物具有高度选择性。与天然acp依赖性途径不同，基于npht7基因的辅酶a依赖性途径产生很少具有c6或更长链长的脂肪酸衍生物。因此，如us2014/0051136中所示，以这种方式修饰的细胞仅产生小比例的c6或更高级脂肪酸衍生物。wo2015/10103描述对npht7酶的修饰，所述修饰允许其更有效地催化长链酰基-辅酶a化合物与丙二酰基-辅酶a的缩合。修饰某些菌株以包含野生型npht7基因和所述基因的变体。这些细胞产生更大相对量的较长脂肪酸，但选择性较差。c6-c10脂肪酸衍生物特别地仅以少量制备。因此，仍然需要以良好产率和对c6-c10脂肪酸或衍生物具有良好选择性产生脂肪酸的方法。申请人已经发现c6-c10脂肪酸或衍生物可通过用突变型3-酮酰基-辅酶a合酶基因修饰细胞来产生，所述基因在细胞中产生某些突变型3-酮酰基-辅酶a合酶。包含子序列seqidno.87、seqidno.105或seqidno.106或与这些中的任一者紧密同源的子序列中的至少一者的天然存在的3-酮酰基-辅酶a合酶当在细胞中异源表达时倾向于主要产生c10或更高级脂肪酸或衍生物，通常伴有显著比例的c12或更高级化合物。本申请人已经发现，通过在这些子序列内进行特定突变，脂肪酸或衍生物的链长向下移动，其中c6-c10链长度变得占优势，并且c12和更高级化合物的产生变得可忽略不计。在特定情况下，观察到对c6和/或c8链长的高选择性。因此，本发明在一方面是一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括以下中的至少一者：a)与seqidno.1具有至少80％同一性的子序列，条件是氨基酸残基8是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸，并且氨基酸残基2是亮氨酸或甲硫氨酸；或与seqidno.160具有至少80％同一性的子序列，条件是氨基酸残基8是半胱氨酸、亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸，并且氨基酸残基2是亮氨酸、苏氨酸或甲硫氨酸；b)与seqidno.2具有至少80％同一性的子序列，条件是氨基酸残基6是异亮氨酸或甲硫氨酸；以及c)与seqidno.3具有至少80％同一性的子序列，条件是氨基酸残基6是异亮氨酸、甲硫氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸。本发明在另一方面是一种经遗传修饰的细胞，所述经遗传修饰的细胞包含编码这种3-酮酰基-辅酶a合酶的异源核酸序列。本申请人进一步发现，在某些异源表达的3-酮酰基-辅酶a合酶中进行特定突变可增加c6至c10链长的脂肪酸(和脂肪酸衍生物)的产生(例如增加的滴度)。因此，在其它方面，本发明是一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括seqidno.50、seqidno.56、seqidno.60、seqidno.65、seqidno.69以及seqidno.170中的一者或多者；以及一种经遗传修饰的细胞，所述经遗传修饰的细胞包含编码这种3-酮酰基-辅酶a合酶的异源核酸序列。本发明在特定方面是具有seqidno.119的突变型3-酮酰基-辅酶a合酶；以及一种经遗传修饰的细胞，所述经遗传修饰的细胞包含编码这种突变型3-酮酰基-辅酶a合酶的异源核酸序列。已发现这些突变型3-酮酰基-辅酶a合酶提高具有直链烷基的化合物的产生以及对此类化合物中的c6-c10链长的特异性。在特定实施方案中，所述突变型3-酮酰基-辅酶a合酶可具有以下中的任一者：seqidno.9、seqidno.10、seqidno.11、seqidno.12、seqidno.13、seqidno.14、seqidno.15、seqidno.16、seqidno.17、seqidno.18、seqidno.19、seqidno.20、seqidno.21、seqidno.22、seqidno.23、seqidno.24、seqidno.25、seqidno.26、seqidno.27、seqidno.28、seqidno.29、seqidno.30、seqidno.31、seqidno.32、seqidno.33、seqidno.34、seqidno.36、seqidno.37、seqidno.38、seqidno.39、seqidno.92、seqidno.93、seqidno121-157中的任一者或seqidno.172-204中的任一者。本发明在其它方面包括使用任何前述方面的微生物来制备具有直链烷基的化合物的方法。在特定方面，本发明是一种用于制备一种或多种具有直链烷基的化合物的方法，所述方法包括在发酵培养基中培养本发明的经遗传修饰的细胞以及从所述发酵培养基中回收具有直链烷基的所述一种或多种化合物。以下表1包括本文提及的3-酮酰基-辅酶a合酶序列和子序列的总结。在表1中，“asch”是指申氏不动杆菌(acinetobacterschindleri)cip107287；“asch-2”是指申氏不动杆菌niph900；“ajoh-2”是指约氏不动杆菌(acinetobacterjohnsii)sh046；“alwo”是指鲁氏不动杆菌(acinetobacterlwoffii)sh145；“aniph71”是指不动杆菌属种niph713；“asch同系物”是指asch、asch-2、ajoh-2、alwo以及aniph71；“pstu”是指施氏假单胞菌(pseudomonαsstutzeri)atcc17588，并且“aagr”是指土壤异希瓦氏菌(alishewanellaagri)bl06。表1—3-酮酰基-辅酶a合酶序列和子序列的总结本文所述的所有氨基酸序列中的氨基酸残基按n-末端至c-末端方向排序。“上游”是指在朝向n-末端的方向上，并且“下游”是指朝向c-末端方向。氨基酸序列的“起始”是在n-末端方向上的第一个氨基酸残基。本文所述的任何序列或子序列的第一个氨基酸残基(氨基酸残基1)是其n-末端处的氨基酸残基。“子序列”是包含在较大氨基酸序列内的氨基酸残基的序列。“同一性”在本文中用于指示两个(核苷酸或氨基酸)序列在比对中在相同位置具有相同残基的程度。除非在本段中另有说明，否则同一性在本文中表述为如使用blast(国家生物信息中心(ncbi)基本局部比对搜索工具(nationalcenterforbiologicalinformation(ncbi)basiclocalalignmentsearchtool))版本2.2.31软件、使用默认参数确定的同一性％。使用蛋白质blast用以下参数测定氨基酸序列之间的同一性：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：6；查询范围内的最大匹配：0；矩阵：blosum62；空位成本：(存在：11，延伸：1)；组合调整：条件组合评分矩阵调整；过滤：无选择；掩码：无选择。使用标准核苷酸blast用以下默认参数测定核酸序列之间的核酸序列同一性％：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：28；查询范围内的最大匹配：0；匹配/错配分数：1，-2；空位成本：线性；过滤：低复杂度区域；掩码：仅用于查找表的掩码。如以这种方式测定的与参考序列具有xx％(例如，80％)的同一性得分％的序列出于本发明的目的被认为与参考序列xx％相同或等同地与参考序列具有xx％序列同一性。出于本发明的目的，当在下列情况时，所研究的序列或子序列的氨基酸残基与参考序列或子序列的氨基酸残基“比对”：i)在整个序列的情况下，使用blast版本2.2.31软件以上述方式对序列进行比对，并且所研究的序列的氨基酸残基在比对中与参考序列的氨基酸残基占据相同的位置；ii)在子序列的情况下，将含有所研究的子序列的序列与参考序列进行比对，并且所研究的子序列的氨基酸残基在比对中与参考子序列的氨基酸残基占据相同的位置。例如，野生型申氏不动杆菌cip107287(seqidno.8)的3-酮酰基-辅酶a合酶包含以下9个氨基酸残基的子序列。氨基酸残基lnlsevdad在子序列中的位置123456789在整个序列中的位置239240241242243244245246247当使用如所述的blast软件将野生型不动杆菌属种niph713(seq.id.no.89)的3-酮酰基-辅酶a合酶与seqidno.8进行比对时，以下氨基酸残基在所述比对中占据相同的位置：不动杆菌属种niph子序列的亮氨酸(l)与申氏不动杆菌子序列的氨基酸残基位置1处的亮氨酸对齐，并且还与申氏不动杆菌3-酮酰基-辅酶a合酶序列的位置239处的亮氨酸对齐。不动杆菌属种niph子序列的缺失氨基酸残基与申氏不动杆菌子序列的氨基酸残基位置6处的缬氨酸(v)对齐，并且还与申氏不动杆菌3-酮酰基-辅酶a合酶序列的胺位置244的缬氨酸对齐。不动杆菌属种niph子序列的第二天冬酰胺(n)残基与申氏不动杆菌子序列的位置7处的天冬氨酸残基对齐，并且还与申氏不动杆菌3-酮酰基-辅酶a合酶序列的位置245处的天冬氨酸残基对齐。出于本申请的目的，如果遗传物质如基因、启动子和终止子是(i)宿主细胞非天然的和/或(ii)是细胞天然的，但存在于不同于其中所述遗传物质存在于野生型宿主细胞中的位置处和/或(iii)处于非天然启动子和/或非天然终止子的调控控制之下，那么它是“异源的”。出于本发明的目的，天然遗传物质的额外拷贝被认为是“异源的”，即使此类额外拷贝与所述遗传物质存在于野生型宿主菌株中的相同基因座处。如果酶(如3-酮酰基-辅酶a合酶)不由野生型宿主细胞产生，则它是“异源的”。“3-酮酰基-辅酶a合酶”是催化酰基-辅酶a与丙二酰基-辅酶a的缩合反应以形成3-酮酰基-辅酶a的酶。可通过使用5,5'-二硫代双-(2-硝基苯甲酸)(dtnb)试剂、丙二酰基-辅酶a作为供体底物(donorsubstrate)以及c4-c8酰基-辅酶a作为引物底物(primersubstrate)在酶存在下测量游离辅酶a-sh的释放来评价所述酶催化这种反应的能力。从任何这些引物底物形成相应的3-酮酰基-辅酶a表明所述酶是3-酮酰基-辅酶a合酶。还可使用丁酰基-辅酶a作为引物底物和丙二酰基-辅酶a作为供体底物、在5mmmg++盐存在下通过测量303nm处的吸光度随着与3-酮己酰基-辅酶a产物形成mg++-络合物的增加的增加来评价酶催化这种反应的能力。3-酮酰基-辅酶a合酶将从丁酰基-辅酶a引物产生3-酮己酰基-辅酶a，从己酰基-辅酶a引物产生3-酮辛酰基-辅酶a，并且从辛酰基-辅酶a引物产生3-酮癸酰基-辅酶a。ec编号由国际生物化学与分子生物学联合会命名委员会(nc-iubmb)确立(enzymenomenclature1992[academicpress,sandiego,california,isbn0-12-227164-5(精装本),0-12-227165-3(平装本)]具有增刊1(1993)、增刊2(1994)、增刊3(1995)、增刊4(1997)以及增刊5(eur.j.biochem.1994,223,1-5；eur.j.biochem.1995,232,1-6；eur.j.biochem.1996,237,1-5；eur.j.biochem.1997,250；1-6以及eur.j.biochem.1999,264,610-650中)。本文引用的ec编号来源于kegg配体数据库，所述数据库由京都基因和基因组学百科全书(kyotoencyclopediaofgenesandgenomics)维护，部分由东京大学赞助。除非另有说明，否则ec编号如截至2009年4月在所述数据库中所提供。出于本发明目的，“脂肪酸”是具有至少四个碳原子的直链一元烷酸。脂肪酸的“衍生物”是在脂肪酸(或相应的辅酶a化合物)的末端羧基位点处的一系列一个或多个反应中形成的具有直链烷基的化合物，所述一个或多个反应将末端羧基(不损失羧基碳)转化为不同的端基，例如像酯、醇基、氨基、醛基、酮、甲基或烯基。脂肪酸的直链烷基的长度保留在任何这种衍生物中，并且在一些情况下可延伸。脂肪酸酯是对应于脂肪酸或脂肪酰基-辅酶a与醇(伴随失水)的反应产物的酯化合物。本文有时用简写“cx”指示脂肪酸和衍生物的链长，其中x是指定碳原子数的数字。在每种情况下指定的碳原子数表示由本发明的细胞通过反应循环的一次或多次重复形成的直链化合物的碳长度(在除去辅酶a或acp辅酶之后)：酰基-辅酶a(或酰基-acp)+丙二酰基-辅酶a形成3-酮酰基化合物；3-酮酰基化合物还原形成3-羟基酰基化合物；3-羟基酰基-辅酶a脱水形成3-烯酰基酰基化合物；以及3-烯酰基酰基化合物还原为相应的酰基化合物。这种反应循环的每次重复将两个碳原子添加至起始酰基-辅酶a或酰基-acp中。碳原子数不包括可在脂肪酸的任何衍生物的形成过程中添加的额外碳原子，例如像酯基中包含的碳。因此，出于本发明的目的，己酸甲酯被认为是“c6”脂肪酸酯化合物，甲酯基团的碳不计数在内。在一些实施方案中，所述经遗传修饰的细胞是原核细胞。在一些实施方案中，所述经遗传修饰的细胞是真核细胞。在一些实施方案中，所述经遗传修饰的细胞是微生物，并且可以是单细胞微生物。宿主细胞可以是植物细胞，包括来自绿藻门(chlorophyta)、轮藻门(charophyta)、地钱门(marchantiophyta)、角苔门(anthocerotophyta)、苔藓植物门(bryophyta)、石松门(lycopodiophyta)、蕨类植物门(pteridophyta)、苏铁门(cycadophyta)、银杏门(ginkgophyta)、松柏门(pinophyta)、买麻藤门(gnetophyta)或被子植物门(magnoliophyta)植物中的任一种内的植物的细胞。这种植物细胞可以是，例如，来自拟南芥属(arabidopsis)、甜菜属(beta)、大豆属(glycine)、麻风树属(jatropha)、芒属(miscanthus)、黍属(panicum)、草芦属(phalaris)、杨属(populus)、甘蔗属(saccharum)、柳属(salix)、希蒙得木属(simmondsia)和玉蜀黍属(zea)中的任一种内的植物的细胞。宿主细胞可以是真菌、微藻、藻类或红藻(长短鞭毛体)细胞。宿主细胞可以是酵母细胞。酵母或真菌细胞可以是产油酵母或真菌，和/或可以是crabtree阴性酵母或真菌。术语“产油真菌”是指酵母或丝状真菌，其累积至少10％、12.5％、15％、17.5％、优选至少20％或甚至至少25％(w/w)的其生物质作为脂质。它们甚至可累积至少30％、40％、50％、60％、70％、80％(w/w)或更多的其生物质作为脂质。生物质通常作为细胞干重(cdw)进行测量。“crabtree阳性”生物体是能够在氧存在下产生乙醇的生物体，而“crabtree阴性”生物体不是能够在氧存在下产生乙醇的生物体。具有crabtree阴性表型的酵母细胞是不显示crabtree效应的任何酵母细胞。术语“crabtree阴性”是指天然存在的和经遗传修饰的生物体两者。简言之，crabtree效应被定义为当在需氧条件下培养时由于高浓度葡萄糖(例如，10g-葡萄糖l-1)的存在而抑制微生物的氧消耗。换句话说，由于葡萄糖的存在，具有crabtree阳性表型的酵母细胞继续发酵而不考虑氧利用率，而具有crabtree阴性表型的酵母细胞不展现葡萄糖介导的氧消耗的抑制。crabtree阳性酵母产生过量的醇而不是产生生物质。合适的酵母细胞的实例包括毕赤酵母属(pichia)、假丝酵母属(candida)、克雷伯氏菌属(klebsiella)、汉逊酵母属(hansenula)、克鲁维酵母属(kluyveromyces)、丝孢酵母属(trichosporon)、酒香酵母属(brettanomyces)、管囊酵母属(pachysolen)、伊萨酵母属(issatchenkia)、yamadazyma酵母属、裂殖酵母属(schizosaccharomyces)、接合酵母属(zygosaccharomyces)、德巴利氏酵母属(debaryomyces)、隐球酵母属(cryptoococcus)、红酵母属(rhodotorula)、红冬孢酵母属(rhodosporidium)、油脂酵母属(lipomyces)以及耶氏酵母属(yarrowia)。具体宿主酵母细胞的实例包括萨纳瑞西斯假丝酵母(c.sonorensis)、马克斯克鲁维酵母(k.marxianus)、耐热克鲁维酵母(k.thermotolerans)、c.methanesorbosa、博伊丁酵母(saccharomycesbulderi)(s.bulderi)、东方伊萨酵母(i.orientalis)、朗比可假丝酵母(c.lambica)、山梨木糖假丝酵母(c.sorboxylosa)、c.zemplinina、c.geochares、膜璞毕赤酵母(p.membranifaciens)、z.kombuchaensis、c.sorbosivorans、c.vanderwaltii、山梨假丝酵母(c.sorbophila)、双孢接合酵母(z.bisporus)、迟缓接合酵母(z.lentus)、贝酵母(saccharomycesbayanus)(s.bayanus)、卡氏德巴利酵母(d.castellii)、博伊丁假丝酵母(c,boidinii)、埃切假丝酵母(c.etchellsii)、乳酸克鲁维酵母(k.lactis)、杰丁毕赤酵母(p.jadinii)、异常毕赤酵母菌(p.anomala)、酿酒酵母(saccharomycescerevisiae)(s.cerevisiae)、盔形毕赤酵母(pichiagaleiformis)、毕赤酵母属yb-4149(nrrl名称)、嗜酒假丝酵母(candidaethanolica)、沙漠毕赤酵母(p.deserticola)、膜璞毕赤酵母(p.membranifaciens)、发酵毕赤酵母(p.fermentans)、圆红冬孢酵母(rhodosporidiumtoruloide)、斯达氏油脂酵母(lipomycesstarkeyii)、产油油脂酵母(l.lipoferus)、拉考夫假丝酵母(candidarevkaufi)、美极梅奇酵母(c.pulcherrima)、热带假丝酵母(c.tropicalis)、产朊假丝酵母(c.utilis)、trichosporonpullas、皮状丝孢酵母(t.cutaneum)、粘红酵母(rhodotorulaglutinous)、禾本红酵母(r.garminis)、解脂耶氏酵母(yarrowialipolytica)以及山楂覆膜孢酵母(saccharomycopsiscrataegensis)(s.crataegensis)。合适的马克斯克鲁维酵母(k.marxianus)和萨纳瑞西斯假丝酵母(c.sonorensis)菌株包括在wo00/71738a1、wo02/42471a2、wo03/049525a2、wo03/102152a2和wo03/102201a2中描述的那些。合适的东方伊萨酵母(i.orientalis)菌株是atcc菌株32196和atcc菌株pta-6648。在一些实施方案中，宿主细胞是细菌细胞。细菌可以是革兰氏阳性或革兰氏阴性细菌。它可以是细菌的衣原体门、绿色非硫、放线菌门、浮霉菌门、螺旋体门、梭杆菌门、蓝菌门、嗜热硫酸盐还原菌、酸杆菌门或变形菌门分类中的任一种内的细胞(ciccarelli等人,science311(5765):1283-7(2006)。合适的细菌细胞的实例包括例如以下属中的任一种内的那些：梭菌属(clostridium)、发酵单胞菌属(zymomonas)、埃希氏菌属(escherichia)、沙门氏菌属(salmonella)、红球菌属(rhodococcus)、假单胞菌属(pseudomonas)、链霉菌属(streptomyces)、芽孢杆菌属(bacillus)、乳杆菌属(lactobacillus)、肠球菌属(enterococcus)、产碱杆菌属(alcaligenes)、克雷伯氏菌属(klebsiella)、类芽孢杆菌属(paenibacillus)、节细菌属(arthrobacter)、棒状杆菌属(corynebacterium)、噬菌体(bacteriophage)、短杆菌属(brevibacterium)、刺菊石属(acanthoceras)、acanthococcus、acarvochloris、曲壳藻属(achnanthes)、细曲壳藻属(achnanthidiun)、星状藻属(actinastrum)、actinochloris、辐环藻属(actinocyclus)、辐射鼓藻属(actinotaenium)、双金藻属(amphichrsis)、前沟藻属(amphidiniunm)、尖粒藻属(amphikrikos)、双肋藻属(amplhipleura)、茧形藻属(amphiprora)、分须藻属(amphithrix)、双眉藻属(amphora)、鱼腥藻属(anabaena)、项圈藻属(anabaenopsis)、aneumnastus、纤维藻属(ankistrodesmius)、锚藻属(ankyra)、异菱藻属(anomoeoneis)、虚幻球藻属(apatococcus)、束丝藻属(aphanizomenon)、隐球藻属(aphanocapsa)、隐毛藻属(aphanochaete)、隐杆藻属(aphanothece)、梨囊藻属(apiocystis)、顶丝藻属(apistonema)、四棘鼓藻属(arthrodesmus)、节旋藻属(artherospira)、ascochloris、星杆藻属(asterionella)、绿星球藻属(asterococcus)、奥杜藻属(audouinella)、沟链藻属(aularoseira)、棍形藻属(bacillaria)、巴尔比亚藻属(balbiania)、似竹鼓藻属(bambiusina)、红毛菜属(bangia)、鞭毛虫属(basichlamys)、串珠藻属(batrarhospermum)、骈胞藻属(binurlearia)、角藻属(bitrichia)、盘苔属(blidingia)、拟气球藻属(botrdiopsis)、气球藻属(botrydium)、葡萄藻属(botryococcus)、球葡萄藻属(botryosphaerella)、咸胞藻属(brachiomonas)、短纹藻属(brachysira)、海雹菜属(brachytrichia)、brebissonia、毛鞘藻属(bulbochaete)、柱杆藻属(bumnilleria)、拟柱杆藻属(buinilleriopsis)、美壁藻属(caloneis)、眉藻属(calothrix)、马鞍藻属(campylodiscus)、盒管藻属(capsosiphon)、四鞭藻属(carteria)、catena、cavinula、刺棘藻属(cenritractus)、centroniella、角藻属(ceratiunt)、角毛藻属(chaetoceros)、chaetochloris、硬毛藻属(chaetomorpha)、卡盾藻属(chaetonella)、毛丝藻属(chaetonemna)、毛楯藻属(chaetopeltis)、胶毛藻属(chaetophora)、毛球藻属(chaetosphaeridium)、管孢藻属(chamaesiphion)、轮藻属(chara)、characiochloris、拟小椿藻属(characiopsis)、小椿藻属(characium)、轮藻目(charales)、缘胞藻属(chilomonas)、厚胞藻属(chlainomonas)、盖毛藻属(chlamydoblepharis)、chlamydocapsa、衣藻属(chlamydomonas)、chlamydomonopsis、衣粘藻属(chlamydomnyxa)、chlamydonephris、chlorangiella、拟绿囊藻属(chlorangiopsis)、小球藻属(chlorella)、绿囊藻属(chlorobotrys)、绿幅藻属(chlorobrachis)、绿点藻属(chlorochytrium)、绿球藻属(chloroccun)、绿胶藻属(chlorogloea)、拟绿胶藻属(chlorogloeopsis)、绿梭藻属(chlorogonium)、绿带藻属(chlorolobion)、拟衣藻属(chloromonas)、吊兰属(chlorophysema)、绿藻门(cholorphyta)、绿胶囊藻属(cholorosaccus)、背包藻属(cholorosarcina)、索囊藻属(choricystis)、色植藻属(chromophyton)、单鞭金藻属(chromulina)、拟甲色球藻(chroococcidiopsis)、色球藻属(chrococcus)、色指藻属(chroodactylon)、蓝隐藻属(chroomonas)、色合藻属(chroothece)、金变形藻属(chrysamoeba)、金网藻属(chysapsis)、金星藻属(chrysidiastrum)、金囊藻属(chrysocapsa)、chrysocapsella、chrysochaete、金色藻属(chrysohromulina)、金颗藻属(chrysococcus)、chrysocrinus、chrynsolepidomonas、chrysolykos、chrysonebula、金藻门(chrysophyta)、金钟藻属(chrysopyxis)、chrysosaccus、chrysophaerella、金环藻属(chrysotephanosphaera)、刚毛藻属(clodophora)、链孢藻属(clastidium)、拟新月藻属(closteriopsis)、新月藻属(closterium)、胶球藻属(coccomyxa)、卵形藻属(cocconeis)、经纹球藻(coelastrella)、空星藻属(coelastrum)、腔球藻属(coelosphaerium)、帽球藻属(coenochloris)、无胶集球藻属(coenococcus)、聚囊藻属(coenocystis)、柄裸藻属(colacium)、鞘毛藻属(coleochaete)、collodictyon、compsogonopsis、弯枝藻属(compsopogon)、conjugatophyta、conoehaete、冠星藻属(coronastrum)、鼓藻属(cosmarium)、cosmnioneis、胶球鼓藻属(cosmocladium)、crateriportula、格形藻属(craticula)、发毛针藻属(crinalium)、十字藻属(crucigenia)、卵胞藻属(crucigeniella)、cryptoaulax、隐藻属(cryptomonas)、隐藻门(cryptophyta)、栉水母门(ctenophora)、蓝网藻属(cyanodictyon)、cyanonephron、蓝载藻属(cyanophora)、蓝藻门(cyanophyta)、蓝杆藻属(cyanothece)、cyanothomonas、环胞藻属(cyclonexis)、环冠藻属(cyclostephanos)、小环藻属(cyclotella)、筒藻属(cylindrocapsa)、柱胞鼓藻属(cylindrocystis)、柱孢藻属(cylindrospermum)、细柱藻属(cylindrotheca)、波缘藻属(cymatopleura)、桥弯藻属(cymbella)、cymbeilonitzschia、胞甲藻属(cystodinium)、蓝纤维藻属(dactylococcopsis)、单板藻属(debarya)、细齿藻属(denticula)、dermatochrysis、皮果藻属(dermorarpa)、小皮果蓝细菌属(dermocarpella)、缢带藻属(desmatractum)、角丝鼓藻属(desmidium)、鼓藻属(desmococcus)、带线藻属(desmonema)、desmosiphon、长刺藻属(diacanthos)、diacronema、等列藻属(diadesmis)、等片藻属(diatoma)、等隔藻属(diatomella)、双细胞藻属(dicellula)、双须藻属(dichothrix)、叉球藻属(dichtotomococcrus)、dicranochaete、网绿藻属(dictyochloris)、球网藻属(dictyococcus)、胶网藻属(dictyosphaerium)、双囊藻属(didymocystis)、对囊藻属(didymogenes)、双楔藻属(didymosphenia)、丝藻属(dilabifilum)、双形藻属(dimorphoccus)、锥囊藻属(dinobryon)、异常球菌属(dinocuccus)、双绿藻属(diplochloris)、双壁藻属(diploneis)、双十藻属(diplostauron)、distrionella、基纹鼓藻属(docidium)、竹枝藻属(draparnaldia)、杜氏藻属(dunaliella)、异形藻属(dysmorphaocuccus)、延胞藻属(ecballocystis)、纺锤藻属(elakatothrix)、ellerbeckia、内丝藻属(encyonema)、浒苔属(enteromorpha)、内枝藻属(entocladia)、苗形藻属(entomoeis)、石囊藻属(entophysalis)、附金藻属(ephichrysis)、附钟藻属(epipyxis)、窗纹藻属(epithemia)、独球藻属(eremosphaura)、拟凹顶藻属(euastropsis)、凹顶鼓藻属(euatstrum)、立方藻属(eucapsis)、真卵形藻属(eucocconeis)、空球藻属(eudorina)、裸藻属(euglena)、裸藻门(euglenophyta)、短缝藻属(eunotia)、眼点藻门(eustigmatophyta)、双鞭藻属(eutreptia)、曲解藻属(fallcia)、侧生藻属(ficherella)、脆杆藻属(fragilaria)、fragilariforma、被棘藻属(franceia)、肋缝藻属(frustulia)、curcilla、双胞藻属(geminella)、短水绵属(genicularia)、灰胞藻属(glaucocystis)、灰藻门属(glaucophyta)、拟薄甲藻属(glenodiniopsis)、薄甲藻属(glenodinium)、胶胞藻属(gloeomonas)、gloeoplax、粘杆藻属(gloeothece)、胶丝藻属(geloeotila)、胶刺藻属(gloeotrichia)、gloiodictyon、多芒藻属(golenkinia)、拟多芒藻属(golenkiniopsis)、孢根藻属(gomontia)、楔桥弯藻属(gomphocymbella)、异极藻属(gomphonema)、束球藻属(gomphosphaeria)、棒形鼓藻属(gonatozygon)、gongrosia、链瘤藻属(gongrosira)、角绿藻属(goniochloris)、盘藻属(gonium)、膝口藻属(gonyostomum)、粒绿藻属(granulochloris)、拟粒囊藻属(granulocystopsis)、groenbladia、裸甲藻属(gymnodiunium)、缢丝鼓藻属(gymnozyga)、布纹藻属(gyrosignma)、红球藻属(haematocuccus)、哥胞藻属(hafniomonas)、hallassia、双尖藻属(hammatoidea)、汉氏藻属(hannaea)、菱板藻属(hantzchia)、软管藻属(hapalosiphon)、haplotaenium、定鞭藻门(haptophyta)、海氏藻属(haslea)、半沟甲藻属(hemidinuim)、hemitonia、鸾壳藻属(heribaudiella)、异毛藻属(heteromastix)、异丝藻属(heterothrix)、黄金色藻纲(hibberdia)、胭脂藻属(hildenbrandia)、隐鞭藻属(hillea)、浩罗藻属(holopedium)、须藻属(homoeothrix)、管鞘藻属(hormanthonema)、皮襟藻属(hormotila)、hyalobrachion、hyalocardium、明盘藻属(hyalodiscus)、透明梭藻属(hyalogonium)、圆丝鼓藻属(hyalotheca)、hydrianum、水胞藻属(hydrococcus)、水鞘藻属(hydrocoleum)、水条藻属(hydrocoryne)、水网藻属(hydrodictyon)、水涟藻属(hydrosera)、水树藻属(hydrurus)、蓝枝藻属(hyella)、膜胞藻属(hymenomonas)、细绿藻属(isthmochloron)、拟丝藻属(johannesbaptistia)、肾粒藻属(juranyiella)、卡氏藻属(karayevia)、尖目艮藻属(kathablepharis)、下甲藻属(katodinium)、金杯藻属(kaphyrion)、角球藻属(keratococcus)、蹄形藻属(kirchneriella)、克里藻属(klebsormidium)、嵩草属(kolbesia)、koliella、komarekia、korshikoviella、kraskella、顶棘藻属(lagerheimia)、烧瓶藻属(lagynion)、丽枝藻属(lamprothamnium)、鱼子菜属(lemanea)、鳞孔藻属(lepocinclis)、细链藻属(leptosira)、lobococcus、lobocystis、叶衣藻属(lobomonas)、泥栖藻属(luticola)、鞘丝藻属(lynbya)、malleochloris、鱼鳞藻属(mallomonas)、mantoniella、射星藻属(marssoniella)、martyana、鞭枝藻属(mastigocloleus)、胸隔藻属(gastogloia)、直链藻属(melosira)、平裂藻属(merismopedia)、中斑藻属(mesostigma)、中带鼓藻属(mesotaenium)、微芒藻属(micractinium)、微星鼓藻属(micrasterias)、微毛藻属(microchaete)、微鞘藻属(microcoleus)、微囊藻属(microcystis)、软壳藻属(microglena)、微胞藻属(micromonas)、微孢藻属(microspora)、小丛藻属(microthamnion)、柄球藻属(mischococcus)、单鞭金藻属(monocrysis)、蒜头藻属(monodus)、monomastix、单针藻属(monoraphidium)、礁膜属(monostroma)、转板藻属(mougeotia)、拟转板藻属(mougeotiopsis)、喙藻属(myochloris)、myromecia、粘囊藻属(myxocarcina)、瓶丝藻属(naegeliella)、微拟球藻属(nannochloris)、类球藻属(nautoccus)、舟形藻属(navicula)、neglectella、长蓖藻属(neidium)、nephroclamys、肾形藻属(nephrocytium)、nephrodiella、肾藻属(nephroselmis)、梭形鼓藻属(netrium)、丽藻属(nitella)、拟丽藻属(nitellopsis)、菱形藻属(nitzschia)、节球藻属(nodularia)、念珠藻属(nostoc)、棕鞭藻属(ochromonas)、鞘藻属(oedogonium)、oligochaetophora、棘接鼓藻属(onychonema)、oocadrium、卵囊藻属(oocrystis)、槌棒藻属(opephora)、黄管藻属(ophiocytium)、orthoseira、颤藻属(oscillartoria)、oxyneis、厚枝藻属(pachycladella)、四集藻属(palmella)、palmodictyon、实球藻属(pnadorina)、pannus、帕拉藻属(paralia)、巴喧氏藻属(pascherina)、paulshulzia、盘星藻属(pediastrum)、柄钟藻属(pedinella)、平藻属(pedinomonas)、指藻属(pedinopera)、海网藻属(pelagodictyon)、柱形鼓藻属(penium)、袋鞭藻属(peranema)、拟多甲藻属(peridiniopsis)、多甲藻属(peridinium)、peronia、岩生藻属(petroneis)、壳衣藻属(phacotus)、扁裸藻属(phacus)、phaeaster、褐皮藻属(phaeodermatium)、褐藻门(phaeophyta)、phaeoshaera、金枝藻属(phaeothamnion)、席藻属(phormidium)、叶楯藻属(phycopeltis)、叶绿藻属(phyllariochloris)、phyllocadium、phyllomitas、羽纹藻属(pinnilaria)、pitophora、盘状藻属(placoneis)、游丝藻属(planctonema)、浮球藻属(planktophaeria)、平面藻属(planothidium)、织线藻属(plectonema)、杂球藻属(pleodorina)、pleurastrum、厚皮藻属(pleurocapsa)、侧枝藻属(pleurocladia)、双盘藻属(pleurodiscus)、斜纹藻属(pleurosigma)、侧链藻属(pleurosira)、宽带鼓藻属(pleurotaenium)、pocillomanas、podohedea、多鞭藻属(polyblepharides)、多胶毛藻属(polychaetophora)、多角藻属(polyedriella)、多突藻属(polyedriopsis)、多角绿藻属(polygoniochloris)、polyepidomanas、polytaenia、素衣藻属(polytoma)、polytomella、紫球藻属(porphyridium)、posteriochromonas、prasinochloris、绿枝藻属(prasinocladus)、绿枝藻门(prasinophyta)、溪菜属(praisola)、原绿藻门(prochlorphyta)、斜纹藻属(psammodictyon)、沙生藻属(psammothidium)、假鱼腥藻属(pseudanabaena)、pseudenoclonium、拟四鞭藻属(psuedocarteria)、pseudochate、pseaudocharacium、pseudococcomyxa、伪胶网藻属(pseudodictyosphaerium)、假金杯藻属(pseudokephyrion)、伪瘤皮藻属(pseudoncobrysa)、伪并联藻属(pseudoquadrigula)、pseudophaerocystis、假十字趾藻属(pseudostaurastrum)、pseudostraurosira、甲藻门(pyrrophyta)、四毛藻属(quadrichloris)、四辧藻属(quadricoccus)、并联藻属(quadrigula)、福球藻属(radiocuccus)、radiobetalum、尖头藻属(raphidiopsis)、raphidocelis、针丝藻属(raphidonema)、绿胞藻门(raphidophyta)、peimeria、棒条藻属(rhadorderma)、杆胞藻属(rhabomonas)、根枝藻属(rhizoclonium)、红胞藻属((rhodomonas)、红藻门(rhodiphyta)、弯楔藻属(rhoicosenia)、棒杆藻属(rhopalodia)、胶须藻属(rivularia)、浅罗氏藻属(rosenvingiella)、罗西藻属(rossithidium)、弯柱鼓藻属(roya)、栅藻属(scenedesmus)、scherffelia、裂衣藻属(schizochlamydella)、裂壁藻属(schizochlamys)、裂线藻属(schizomeris)、裂须藻属(schizothrix)、弓形藻属(schroederia)、scolioneis、螺翼藻属(scotiella)、scotiellopsis、斯氏藻属(scourfieldia)、伪枝藻属(scytonema)、月牙藻属(slenastrum)、月绿藻属(selenochloris)、鞍型藻属(sellaphora)、semiorbis、褐胞藻属(siderocelis)、拟铁囊藻属(diderocystopsis)、dimonsenia、管线藻属(siphononema)、链枝藻属(sirocladium)、链膝藻属(sirogonium)、骨条藻属(skeletonema)、群星藻属(sorastrum)、spermatozopis、sphaerellocystis、拟球藻属(sphaerellopsis)、sphaerodinium、环藻属(sphaeroplea)、瘤接鼓藻属(sphaerozosma)、刺胞藻属(spiniferomonas)、水绵属(spirogyra)、螺带鼓藻属(spirotaenia)、螺旋藻属(spirulina)、椎葚属(spondylomorum)、顶接鼓藻属(spondylosium)、sporotetras、泡沫藻属(spumella)、角星鼓藻属(staurastrum)、叉链藻属(stauerodesmus)、辐节藻属(stauroneis)、十字脆杆藻亚属(staurosira)、staurrosiella、长羽藻属(stenopterobia)、stephanocostis、冠盘藻属(stephanodiscus)、冠孔藻属(stephanoporos)、stephanoshaera、裂丝藻属(stichoccus)、粘胶藻属(stichogloea)、毛枝藻属(sigeoclonium)、真枝藻属(stigonema)、柄球藻属(stipitocuccus)、斯鞭虫属(stokesiella)、陀螺藻属(stombomonas)、柄胞藻属(stylochrysalis)、柄甲藻属(stylodinium)、柱钟藻属(styloyxis)、绿柄球藻属(stylosphaeridium)、双菱藻属(surirella)、sykidion、束藻属(symploca)、聚球藻属(synechococcus)、集胞藻属(synechocystis)、针杆藻属(synedra)、聚赫胞藻属(synochromonas)、黄群藻属(synura)、平板藻属(tabellaria)、tabularia、泰林鼓藻属(teilingia)、切孢藻属(temnogametum)、裂顶鼓藻属(tetmemorus)、四球藻属(tetrachlorella)、四环藻属(tetracyclus)、四链藻属(tetrademus)、骈列藻属(tetraedriella)、四角藻属(tetraedron)、四爿藻(tetraselmis)、四孢藻属(tetraspora)、四星藻属(tetrastrum)、海链藻属(thalassiosira)、丛毛藻属(thanmiochaete)、胸甲藻属(thoakochloris)、红索藻属(thorea)、鸟巢藻属(tolypella)、单歧藻属(tolypothrix)、囊裸藻属(trachelomonas)、粗盘藻属(trachydiscus)、共球藻属(trebouxia)、橘色藻属(trentepholia)、四刺藻属(treubaria)、黄丝藻属(tribonema)、束毛藻属(trichodesmium)、tricodiscus、小箍藻属(trochiscia)、盘杆藻属(tryblionella)、丝藻属(ulothrix)、辐尾藻属(uroglena)、尾丝藻属(uronema)、尾管藻属(urosolenia)、尾孢藻属(urospora)、uva、周泡藻属(vacuolaria)、无隔藻属(vaucheria)、团藻属(volvox)、似团藻属(volvulina)、韦斯藻属(westella)、网甲藻属(woloszynskia)、多棘鼓藻属(xanthidium)、黄藻门(xanthophyta)、exencoccus、双星藻属(zygenema)、拟双星藻属(zygnemopsis)以及zygonium。细菌宿主细胞的具体实例包括大肠杆菌、食羧寡养菌(oligotrophacarboxidovorans)、假单胞菌属真养产碱杆菌(alcaligeneseutrophus)(钩虫贪铜菌(cupriavidusnecator))、地衣芽孢杆菌(bacilluslicheniformis)、多粘类芽孢杆菌(paenibacillusmacerans)、红串红球菌(rhodococcuserythropolis)、恶臭假单胞菌(pseudomonasputida)、植物乳杆菌(lactobacillusplantarum)、屎肠球菌(enterococcusfaecium)、鹑鸡肠球菌(enterococcusgallinarium)、粪肠球菌(enterococcusfaecalis)、枯草芽孢杆菌(bacillussubtilis)、巴塞尔贪铜菌(cupriavidusbasilensis)、肯彭贪铜菌(cupriaviduscampinensis)、基氏贪铜菌(cupriavidusgilardi)、cupriaviduslaharsis、耐重金属贪铜菌(cupriavidusmetallidurans)、草酸盐贪铜菌(cupriavidusoxalaticus)、罕见贪铜菌(cupriaviduspauculus)、cupriaviduspinatubonensis、呼吸系统贪铜菌(cupriavidusrespiraculi)、台湾贪铜菌(cupriavidustaiwanensis)、耻垢分枝杆菌(mycobacteriumsmegmatis)、脓肿分枝杆菌(mycobacteriumabscessus)、鸟分枝杆菌(mycobacteriumavium)、牛分枝杆菌(mycobacteriumbovis)、结核分支杆菌(mycobacteriumtuberculosis)、麻风分枝杆菌(mycobacteriumleprae)、海洋分枝杆菌(mycobacteriummarinum)、以及溃疡分枝杆菌(mycobacteriumulcerans)。在其它实施方案中，细菌是诺卡氏菌属种nrrl5646、皮疽诺卡菌(nocardiafarcinica)、灰色链霉菌(streptomycesgriseus)、海洋放线菌(salinisporaarenicola)或密执岁棍状杆菌(clavibactermichiganenesis)。宿主细胞可以是合成细胞或由合成基因组产生的细胞，如美国专利公布2007/0264688或2007/0269862中所描述。宿主细胞可以是cho细胞、cos细胞、vero细胞、bhk细胞、hela细胞、cv1细胞、mdck细胞、293细胞、3t3细胞或pc12细胞。在某些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.1或seqidno.160，或与seqidno.1或seqidno.160具有至少80％同一性的子序列，条件是在每种情况下与氨基酸残基8对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的任一者，并且与氨基酸残基8对齐的氨基酸残基在seqidno.1的情况下是亮氨酸或甲硫氨酸并且在seqidno.160的情况下是亮氨酸、甲硫氨酸或苏氨酸。seqidno.1与seqidno.87的不同之处在于seqidno.87的氨基酸残基位置8中的苏氨酸在seqidno.1中被亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的任一者替代。seqidno.160与seqidno.87的不同之处在于seqidno.87的氨基酸残基位置8中的苏氨酸在seqidno.160中被亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的任一者替代，并且位置2中的氨基酸残基也可以是苏氨酸。seqidno.87表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.87例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基177-185，氨基酸残基178为甲硫氨酸)；申氏不动杆菌niph900(作为seqidno.86的氨基酸残基177-185，氨基酸残基178为甲硫氨酸)；约氏不动杆菌sh046(作为seqidno.35的氨基酸残基177-185，氨基酸残基178为亮氨酸)；鲁氏不动杆菌sh145(作为seqidno.88的氨基酸残基177-185，氨基酸残基178为亮氨酸)；不动杆菌属种niph713(作为seqidno.89的氨基酸残基177-185，氨基酸残基178为亮氨酸)。在子序列seqidno.1或seqidno.160的氨基酸残基8处指示的苏氨酸的这种取代已被发现使脂肪酸产生(和脂肪酸衍生物的产生)转向c6、c8和/或c10脂肪酸和衍生物。3-酮酰基-辅酶a合酶在一些实施方案中包含选自a)seqidno.4或seqidno.161、b)seqidno.5以及c)seqidno.6或seqidno.162的至少一个子序列。3-酮酰基-辅酶a合酶优选地包含a)、b)和c)中的每一个。在其中seqidno.4或seqidno.161存在于3-酮酰基-辅酶a合酶中的一些实施方案中，子序列seqidno.4存在于子序列seqidno.1或seqidno.160(视情况而定)的上游。子序列seqidno.4或seqidno.161(视情况而定)的起始在这种3-酮酰基-辅酶a合酶中可位于例如子序列seqidno.1或seqidno.160(视情况而定)的起始上游的25至30个氨基酸残基、尤其是正好27个氨基酸残基处。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.44和/或seqidno.45，或与那些中的任一者具有至少85％同一性的子序列。如可通过比较序列看出，seqidno.44和45中的每一个包含seqidno.4(其作为seqidno.44和45的氨基酸残基1-7出现)和seqidno.1(其作为seqidno.44和45的氨基酸残基28-36出现)。在子序列seqidno.44和45中，子序列seqidno.4的起始位于子序列seqidno.1的起始上游的27个氨基酸残基处。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.164和/或seqidno.165，或与那些中的任一者具有至少85％同一性的子序列。如可通过比较序列看出，seqidno.164和165中的每一个包含seqidno.161(其作为seqidno.164和165的氨基酸残基1-7出现)和seqidno.160(其作为seqidno.164和165的氨基酸残基28-36出现)。在子序列seqidno.164和165中，子序列seqidno.161的起始位于子序列seqidno.160的起始上游的27个氨基酸残基处。在其中seqidno.5存在于3-酮酰基-辅酶a合酶中的一些实施方案中，子序列seqidno.5优选地存在于子序列seqidno.1或seqidno.160(视情况而定)的下游。子序列seqidno.5的起始在这种3-酮酰基-辅酶a合酶中可位于例如子序列seqidno.1或seqidno.160(视情况而定)的起始下游的100至120个氨基酸残基、108至116个氨基酸残基或正好112或113个氨基酸处。在其中seqidno.6或seqidno.162存在于3-酮酰基-辅酶a合酶中的一些实施方案中，子序列seqidno.6或seqidno.162(视情况而定)优选地存在于子序列seqidno.1或seqidno.160(视情况而定)的下游。子序列seqidno.6或seqidno.162的起始在这种3-酮酰基-辅酶a合酶中可位于例如子序列seqidno.1或seqidno.160(视情况而定)的起始下游的140至160个氨基酸残基、145至155个氨基酸残基、尤其是正好147或148个氨基酸残基处。在其中子序列seqidno.5和6两者均存在的一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.46。在seqidno.46中，子序列seqidno.5作为氨基酸残基1-7出现，并且子序列seqidno.6作为氨基酸残基36-42出现。在其中子序列seqidno.5和162两者均存在的一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.166。在seqidno.166中，子序列seqidno.5作为氨基酸残基1-7出现，并且子序列seqidno.162作为氨基酸残基36-42出现。在其中子序列seqidno.4、5和6中的每一个均存在的一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.47。在seqidno.47中，子序列seqidno.4作为氨基酸残基1-7出现；子序列seqidno.1作为氨基酸残基28-36出现；子序列seqidno.5作为氨基酸残基141-147出现，并且子序列seqidno.6作为氨基酸残基176-182出现。在其中子序列seqidno.161、5和162中的每一个均存在的一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.167。在seqidno.167中，子序列seqidno.161作为氨基酸残基1-7出现；子序列seqidno.160作为氨基酸残基28-36出现；子序列seqidno.5作为氨基酸残基141-147出现，并且子序列seqidno.162作为氨基酸残基176-182出现。在任何前述实施方案中，3-酮酰基-辅酶a合酶可包含子序列seqidno.48或seqidno.168。seqidno.48或seqidno.168优选地位于子序列seqidno.1或seqidno.160(视情况而定)的上游以及子序列seqidno.4或161、5和6或162中的每一个(当存在时)的上游。在一些实施方案中，3-酮酰基-辅酶a合酶包含seqid.no49或与seqidno.49具有至少80％、至少90％或至少95％同一性的序列，条件是与seqidno.49的位置184对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的一者。在一些实施方案中，3-酮酰基-辅酶a合酶包含seqidno.169或与seqidno.169具有至少80％、至少90％或至少95％同一性的序列，条件是与seqidno.169的位置184对齐的氨基酸残基是半胱氨酸、亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的一者。在一些实施方案中，3-酮酰基-辅酶a合酶具有seqid.no110或与seqidno.110具有至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a的与seqidno.110的氨基酸残基184对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的一者。在一些实施方案中，3-酮酰基-辅酶a合酶具有seqidno.119或与seqidno.119具有至少80％、至少90％或至少95％同一性的序列，条件是与seqidno.119的位置184对齐的氨基酸残基是半胱氨酸、亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的一者。在其它实施方案中，3-酮酰基-辅酶a合酶与seqidno.8、35、86、88或89中的任一者具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是在每种情况下3-酮酰基-辅酶a合酶的与seqidno.8、35、86、88或89中的任一者的氨基酸残基184对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸。在具体实施方案中，3-酮酰基-辅酶a合酶具有seqidno.9-34、36-39、92-93、121-157或172-204中的任一者或与seqidno.9-34、36-39、92-93、121-157或172-204中的任一者具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是在每种情况下3-酮酰基-辅酶a的与seqidno.9-34、36-39、92-93、121-157或172-204中的任一者的氨基酸残基184对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸中的一者。在某些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.2或与seqidno.2具有至少80％同一性的序列，条件是3-酮酰基-辅酶a的与seqidno.2的氨基酸残基6对齐的氨基酸残基是异亮氨酸或甲硫氨酸。在其它方面与seqidno.2相同、但是其中与seqidno.2的氨基酸残基6对齐的氨基酸残基是半胱氨酸的子序列出现在天然存在的3-酮酰基-辅酶a合酶中，如施氏假单胞菌atcc17588的3-酮酰基-辅酶a合酶(seqidno.90，其中这种序列作为氨基酸残基181-190出现(其中半胱氨酸作为氨基酸残基186出现))。已发现半胱氨酸的这种取代使脂肪酸产生(和脂肪酸衍生物的产生)转向c6、c8和/或c10脂肪酸和衍生物，并且远离更长链化合物。如之前，包含子序列seqidno.2的3-酮酰基-辅酶a合酶可包含选自a)seqidno.4或seqidno.161、b)seqidno.5以及c)seqidno.6或seqidno.162的至少一个子序列。3-酮酰基-辅酶a合酶优选地包含这些子序列中的每一个。当子序列seqidno.4或seqidno.161存在时，子序列seqidno.4或seqidno.161存在于子序列seqidno.2的上游，并且其起始可位于例如子序列seqidno.2的起始上游的25至35个氨基酸残基、28至32个氨基酸残基或尤其是正好30个氨基酸残基处。当子序列seqidno.5存在时，子序列seqidno.5优选地存在于子序列seqidno.2的下游，并且子序列seqidno.5的起始可位于例如子序列seqidno.2的起始下游的100至120个氨基酸残基、110至118、尤其是正好115或116个氨基酸残基处。当子序列seqidno.6或seqidno.162存在时，子序列seqidno.6或seqidno.162优选地存在于子序列seqidno.2的下游，并且子序列seqidno.6或seqidno.162的起始在这种3-酮酰基-辅酶a合酶中可位于例如子序列seqidno.2的起始下游的140至160个氨基酸残基、145至155个氨基酸残基、尤其是正好148、149或150个氨基酸残基处。在其中子序列seqidno.2存在的一些实施方案中，3-酮酰基-辅酶a合酶与seqidno.40具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a合酶的与seqidno.40的氨基酸残基186对齐的氨基酸残基是异亮氨酸或甲硫氨酸。在某些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.3或与seqidno.3具有至少80％同一性的序列，条件是3-酮酰基-辅酶a的与seqidno.3的氨基酸残基6对齐的氨基酸残基是异亮氨酸、甲硫氨酸、亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺和酪氨酸中的任一者。在其它方面与seqidno.3相同、但是其中与seqidno.3的氨基酸残基6对齐的氨基酸残基是丙氨酸的子序列出现在天然存在的3-酮酰基-辅酶a合酶中，如土壤异希瓦氏菌bl06的3-酮酰基-辅酶a合酶(seqidno.91，其中这种序列作为氨基酸残基181-190出现(其中丙氨酸作为氨基酸残基186出现)。已发现丙氨酸的这种取代大大减少了c12脂肪酸和衍生物的产生，有利于c6、c8和/或c10脂肪酸和衍生物。如之前，包含子序列seqidno.3的3-酮酰基-辅酶a合酶可包含选自a)seqidno.4或seqidno.161、b)seqidno.5以及c)seqidno.6或seqidno.162的至少一个子序列。3-酮酰基-辅酶a合酶优选地包含这些子序列中的每一个。当子序列seqidno.4或seqidno161存在时，子序列seqidno.4或seqidno.161存在于子序列seqidno.3的上游，并且其起始可位于例如子序列seqidno.3的起始上游的25至35个氨基酸残基、28至32个氨基酸残基或尤其是正好30个氨基酸残基处。当子序列seqidno.5存在时，子序列seqidno.5优选地存在于子序列seqidno.3的下游，并且子序列seqidno.5的起始可位于例如子序列seqidno.3的起始下游的100至120个氨基酸残基、110至118、尤其是正好115或116个氨基酸残基处。当子序列seqidno.6或seqidno.162存在时，子序列seqidno.6或seqidno.162优选地存在于子序列seqidno.3的下游，并且子序列seqidno.6或seqidno.162的起始在这种3-酮酰基-辅酶a合酶中可位于例如子序列seqidno.3的起始下游的140至160个氨基酸残基、145至155个氨基酸残基、尤其是正好148、149或150个氨基酸残基处。在其中子序列seqidno.3存在的一些实施方案中，3-酮酰基-辅酶a合酶与seqidno.42中的任一者具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a合酶的与seqidno.42的氨基酸残基186对齐的氨基酸残基是异亮氨酸、甲硫氨酸、亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺和酪氨酸中的任一者。在其中子序列seqidno.3存在的一些实施方案中，3-酮酰基-辅酶a合酶与seqidno.43中的任一者具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a合酶的与seqidno.43的氨基酸残基186对齐的氨基酸残基是异亮氨酸。在其中子序列seqidno.3存在的一些实施方案中，3-酮酰基-辅酶a合酶具有seqidno.42或seqidno.43。在其中子序列seqidno.3存在的一些实施方案中，3-酮酰基-辅酶a合酶具有seqidno.42，其中：a)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者；b)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，并且氨基酸残基241是甲硫氨酸、苯丙氨酸、谷氨酸、亮氨酸、酪氨酸或天冬氨酸中的任一者；c)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，并且氨基酸残基239是谷氨酰胺、天冬氨酸或天冬酰胺中的任一者；d)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，并且氨基酸残基246是赖氨酸或精氨酸中的任一者；e)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，氨基酸残基239是谷氨酰胺、天冬氨酸或天冬酰胺中的任一者，并且氨基酸残基241是甲硫氨酸、苯丙氨酸、谷氨酸、亮氨酸、酪氨酸或天冬氨酸中的任一者；f)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，氨基酸残基239是谷氨酰胺、天冬氨酸、酪氨酸或天冬酰胺中的任一者，并且氨基酸残基246是亮氨酸和精氨酸中的任一者；g)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，氨基酸残基241是甲硫氨酸、苯丙氨酸、谷氨酸或天冬氨酸中的任一者，并且氨基酸残基246是赖氨酸和精氨酸中的任一者；或h)氨基酸残基186是异亮氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸中的任一者，氨基酸残基243是谷氨酸，并且氨基酸残基246是组氨酸、赖氨酸和精氨酸中的任一者。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqid.no.170，或与seqid.no.170具有至少80％同一性的子序列，条件是(1)3-酮酰基-辅酶a的与seqidno.170的氨基酸残基1对齐的氨基酸残基是丙氨酸、半胱氨酸、天冬氨酸、谷氨酸、苯丙氨酸、组氨酸、异亮氨酸、赖氨酸、亮氨酸、甲硫氨酸、天冬酰胺、脯氨酸、谷氨酰胺、丝氨酸、苏氨酸、色氨酸或酪氨酸中的任一者，并且(2)3-酮酰基-辅酶a的与seqidno.170的氨基酸残基4对齐的氨基酸残基是缬氨酸或丙氨酸。seqidno.170与seqidno.171的不同之处在于seqidno.171的氨基酸位置1中的甘氨酸在seqidno.170中被丙氨酸、半胱氨酸、天冬氨酸、谷氨酸、苯丙氨酸、组氨酸、异亮氨酸、赖氨酸、亮氨酸、甲硫氨酸、天冬酰胺、脯氨酸、谷氨酰胺、丝氨酸、苏氨酸、色氨酸或酪氨酸中的任一者替代，并且进一步在于位置4中的氨基酸可以是缬氨酸。seqidno.171表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.171例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基51-60，其中氨基酸残基55为谷氨酸)；申氏不动杆菌niph900(作为seqidno.86的氨基酸残基51-60，其中氨基酸残基55为谷氨酸)；约氏不动杆菌sh046(作为seqidno.35的氨基酸残基51-60，其中氨基酸残基55为谷氨酸)；鲁氏不动杆菌sh145(作为seqidno.88的氨基酸残基51-60，其中氨基酸残基5为天冬氨酸)；不动杆菌属种niph713(作为seqidno.89的氨基酸残基51-60，其中氨基酸残基55为谷氨酸)。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.50或与seqidno.50具有至少80％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.50的氨基酸残基4对齐的氨基酸残基是精氨酸。seqidno.50与seqidno.108的不同之处在于seqidno.108的赖氨酸在seqidno.50中被精氨酸替代。seqidno.108表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.108例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基275-281，其中氨基酸残基280为谷氨酰胺)；申氏不动杆菌niph900(作为seqidno.86的氨基酸残基275-281，其中氨基酸残基279为谷氨酰胺)；约氏不动杆菌sh046(作为seqidno.35的氨基酸残基275-281，其中氨基酸残基280为天冬酰胺)；鲁氏不动杆菌sh145(作为seqidno.88的氨基酸残基274-280，其中氨基酸残基279为谷氨酰胺)；不动杆菌属种niph713(作为seqidno.89的氨基酸残基274-280，其中氨基酸残基279为谷氨酰胺)。已经发现赖氨酸的这种取代增加脂肪酸产生速率(和脂肪酸衍生物的产生速率)。在一些实施方案中，包含子序列seqidno.50的3-酮酰基-辅酶a合酶包含选自seqidno.51、seqidno.4、seqidno.5和seqidno.6的至少一个子序列或其任何两个或更多个的任何组合。3-酮酰基-辅酶a合酶优选地包含子序列seqidno.51、4、5和6中的每一个。当子序列seqidno.4存在时，它优选地位于子序列seqidno.50的上游。子序列seqidno.4的起始可以是例如子序列seqidno.50的起始上游的120至130、122至128或124至125个氨基酸残基。当子序列seqidno.51存在时，它优选地位于子序列seqidno.50的上游。子序列seqidno.51的起始可以是例如子序列seqidno.50的起始上游的93至103、95至100或97至98个氨基酸残基。当子序列seqidno.5存在时，它优选地位于子序列seqidno.50的下游。子序列seqidno.5的起始可以是例如子序列seqidno.50的起始下游的10至20、12至18或正好15个氨基酸残基。当子序列seqidno.6存在时，它优选地位于子序列seqidno.50的下游。子序列seqidno.5的起始可以是例如子序列seqidno.50的起始下游的45至56、49至53或正好51个氨基酸残基。包含子序列seqidno.50的3-酮酰基-辅酶a合酶还可包含子序列seqidno.52、子序列seqidno.53、子序列seqidno.46、子序列seqidno.48(条件是氨基酸残基278为精氨酸)或子序列seqidno.110(再次条件是氨基酸残基278为精氨酸)中的任何一个或多个。所述子序列seqidno.52或53的起始可以是子序列seqidno.50的起始上游的120至130、122至128或124至126个氨基酸残基。子序列seqidno.52的起始可以是子序列seqidno.50的起始上游的120至130、122至128或124至126个氨基酸残基。子序列seqidno.46的起始可以是例如子序列seqidno.50的起始下游的10至20、12-18或正好15个氨基酸残基。包含子序列seqidno.50的3-酮酰基-辅酶a合酶可包含子序列seqidno.54，条件是seqidno.54的氨基酸残基129是精氨酸。包含seqidno.50的3-酮酰基-辅酶a合酶子序列可包含seqidno.55，或者包含与seqidno.55具有至少50％、至少75％、至少80％、至少90％或至少95％同一性的序列，条件是3-酮酰基-辅酶a的与seqidno.55中的氨基酸残基278对齐的氨基酸残基是精氨酸。包含seqidno.50的3-酮酰基-辅酶a合酶子序列可具有seqidno.111，或者与seqidno.111具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a的与seqidno.111中的氨基酸残基278对齐的氨基酸残基是精氨酸。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.56或与seqidno.56具有至少80％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.56的氨基酸残基6对齐的氨基酸残基是丙氨酸。如将通过比较序列看出，seqidno.56与seqidno.109的不同之处在于seqidno.109的氨基酸残基位置6中的缬氨酸在seqidno.56中被丙氨酸替代。seqidno.109表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.109例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基291-300)和申氏不动杆菌niph900(作为seqidno.86的氨基酸残基291-300)。已经发现丙氨酸对缬氨酸的这种取代增加脂肪酸产生速率(和脂肪酸衍生物的产生速率)。在一些实施方案中，包含子序列seqidno.56的3-酮酰基-辅酶a合酶包含选自seqidno.51、seqidno.4和seqidno.6的至少一个子序列或其任何两个或更多个的任何组合。3-酮酰基-辅酶a合酶优选地包含子序列seqidno.51、4和6中的每一个。子序列seqidno.51的起始可以是子序列seqidno.56的起始上游的109至120、112至116或114个氨基酸残基。子序列seqidno.4的起始可以是子序列seqidno.56的起始上游的135至148、139至143或141个氨基酸残基。子序列seqidno.6的起始可以是子序列seqidno.56的起始下游的30至40、33至37或35个氨基酸残基。包含子序列seqidno.56的3-酮酰基-辅酶a合酶还可包含子序列seqidno.52、子序列seqidno.53或子序列seqidno.48中的任何一个或多个。子序列seqidno.52或53的起始可以是子序列seqidno.56的起始上游的135至148、139至143或141个氨基酸残基。子序列seqidno.48的起始可以是子序列seqidno.56的起始上游的285至295、288至292或290个氨基酸残基。包含子序列seqidno.56的3-酮酰基-辅酶a合酶可包含子序列seqidno.58。包含seqidno.56的3-酮酰基-辅酶a合酶子序列可具有seqidno.59，或者与seqidno.59具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a的与seqidno.59中的氨基酸残基296对齐的氨基酸残基是丙氨酸。包含seqidno.56的3-酮酰基-辅酶a合酶子序列可具有seqidno.112，或者与seqidno.112具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a的与seqidno.112中的氨基酸残基296对齐的氨基酸残基是丙氨酸。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqid.no.60，或与seqid.no.60具有至少80％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.60的氨基酸残基2对齐的氨基酸残基是亮氨酸，并且3-酮酰基-辅酶a的与seqidno.60的氨基酸残基10对齐的氨基酸残基是谷氨酰胺。如将通过比较序列看出，seqidno.60与seqidno.116的不同之处在于seqidno.116的氨基酸残基位置2中的甲硫氨酸在seqidno.60中被亮氨酸替代。seqidno.116表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.116例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基177-190)和申氏不动杆菌niph900(作为seqidno.86的氨基酸残基177-190)。已经发现亮氨酸对甲硫氨酸的这种取代增加脂肪酸产生速率(和脂肪酸衍生物的产生速率)。包含子序列seqidno.60的3-酮酰基-辅酶a合酶可进一步包含子序列seqidno.4。子序列seqidno.4的起始可以是子序列seqidno.60的起始上游的22至32、25至29或27个氨基酸残基。这种3-酮酰基-辅酶a合酶可具有子序列seqidno.61或子序列seqidno.62。在一些实施方案中，包含子序列seqidno.60的3-酮酰基-辅酶a合酶包含选自seqidno.5和seqidno.6的至少一个子序列。子序列seqidno.5的起始可以是子序列seqidno.60的起始下游的109至120、11至115或112至113个氨基酸残基。子序列seqidno.6的起始可以是子序列seqidno.60的起始上游的143至153、146至151或148至149个氨基酸残基。3-酮酰基-辅酶a合酶优选地包含子序列seqidno.4、5和6中的每一个。包含子序列seqidno.60、seqidno.4、seqidno.5和seqidno.6的3-酮酰基-辅酶a合酶可包含子序列seqidno.63。包含子序列seqidno.60的3-酮酰基-辅酶a合酶还可包含子序列seqidno.48。子序列seqidno.48的起始可以是子序列seqidno.60的起始上游的173至183、175至179或177个氨基酸残基。包含seqidno.60的3-酮酰基-辅酶a合酶子序列可包含seqidno.64，或者包含与seqidno.64具有至少50％、至少75％、至少80％、至少90％或至少95％同一性的序列，条件是位置178中的氨基酸残基是亮氨酸，并且位置186中的氨基酸残基是谷氨酰胺。包含seqidno.60的3-酮酰基-辅酶a合酶子序列可具有seqidno.113，或者与seqidno.113具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是位置178中的氨基酸残基是亮氨酸，并且位置186中的氨基酸残基是谷氨酰胺。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.65或与seqidno.65具有至少80％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.65的位置2中的氨基酸残基对齐的氨基酸残基是丙氨酸。如将通过比较序列看出，seqidno.65与seqidno.97的不同之处在于seqidno.97的氨基酸残基位置2中的缬氨酸在seqidno.65中被丙氨酸替代。seqidno.97表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.97例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基316-331，其中氨基酸残基319为亮氨酸并且氨基酸残基322为天冬氨酸)；申氏不动杆菌niph900(作为seqidno.86的氨基酸残基316-331，其中氨基酸残基319为亮氨酸并且氨基酸残基322为天冬氨酸)；约氏不动杆菌sh046(作为seqidno.35的氨基酸残基316-331，其中氨基酸残基319为异亮氨酸并且氨基酸残基322为天冬氨酸)；鲁氏不动杆菌sh145(作为seqidno.88的氨基酸残基315-330，其中氨基酸残基318为异亮氨酸并且氨基酸残基321为天冬酰胺)；不动杆菌属种niph713(作为seqidno.89的氨基酸残基315-330，其中氨基酸残基318为甲硫氨酸并且氨基酸残基321为天冬酰胺)。已经发现用丙氨酸对缬氨酸的这种取代增加脂肪酸产生速率(和脂肪酸衍生物的产生速率)。包含子序列seqidno.65的3-酮酰基-辅酶a合酶可进一步包含子序列seqidno.5。子序列seqidno.65的起始可以是子序列seqidno.65的起始上游的20至31、24至28或26个氨基酸残基。包含子序列seqidno.65和子序列seqidno.5的3-酮酰基-辅酶a合酶可包含子序列seqidno.66。包含子序列seqidno.65的3-酮酰基-辅酶a合酶可进一步包含子序列seqidno.51。子序列seqidno.51的起始可以是子序列seqidno.65的起始上游的134-144、136至140或138至139个氨基酸残基。包含子序列seqidno.65的3-酮酰基-辅酶a合酶可包含子序列seqidno.4。包含子序列seqidno.65和子序列seqidno.4的3-酮酰基-辅酶a合酶可包含子序列seqidno.53或子序列seqidno.67。子序列seqidno.4、53或67的起始可以是子序列seqidno.65的起始上游的160至170、163至168或165至166个氨基酸残基。包含子序列seqidno.65的3-酮酰基-辅酶a合酶可进一步包含子序列seqidno.48。子序列seqidno.48的起始可以是子序列seqidno.65的起始上游的310至320、313至316或314至315个氨基酸残基。包含子序列seqidno.65的3-酮酰基-辅酶a合酶可包含子序列seqidno.68，或者与seqidno.68具有至少50％、至少75％、至少80％、至少90％或至少95％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.68的氨基酸残基317对齐的氨基酸残基是丙氨酸。包含子序列seqidno.65的3-酮酰基-辅酶a合酶可具有seqidno.114，或者与seqidno.114具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a的与seqidno.114的氨基酸残基317对齐的氨基酸残基是丙氨酸。在一些实施方案中，3-酮酰基-辅酶a合酶包含子序列seqidno.69或与seqidno.69具有至少80％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.69的位置6中的氨基酸残基对齐的氨基酸残基是异亮氨酸。如将通过比较序列看出，seqidno.69与seqidno.107的不同之处在于seqidno.107的氨基酸残基位置6中的甲硫氨酸在seqidno.69中被异亮氨酸替代。seqidno.107表示在一系列天然存在的3-酮酰基-辅酶a合酶中发现的子序列。seqidno.107例如在以下各项的天然存在的3-酮酰基-辅酶a合酶中出现：申氏不动杆菌cip107287(作为seqidno.8的氨基酸残基266-274，其中氨基酸残基268为缬氨酸并且氨基酸残基274为赖氨酸)；申氏不动杆菌niph900(作为seqidno.86的氨基酸残基266-274，其中氨基酸残基268为缬氨酸并且氨基酸残基274为赖氨酸)；约氏不动杆菌sh046(作为seqidno.35的氨基酸残基266-274，其中氨基酸残基268为缬氨酸并且氨基酸残基274为丙氨酸)；鲁氏不动杆菌sh145(作为seqidno.88的氨基酸残基265-273，其中氨基酸残基267为缬氨酸并且氨基酸残基273为丙氨酸)；不动杆菌属种niph713(作为seqidno.89的氨基酸残基265-273，其中氨基酸残基267为缬氨酸并且氨基酸残基273为丙氨酸)。已经发现用异亮氨酸对甲硫氨酸的这种取代增加脂肪酸产生速率(和脂肪酸衍生物的产生速率)。包含子序列seqidno.69的3-酮酰基-辅酶a合酶可进一步包含子序列seqidno.51。子序列seqidno.51的起始可以是子序列seqidno.69的起始上游的85至95、87至91或88至89个氨基酸残基。包含子序列seqidno.69的3-酮酰基-辅酶a合酶可进一步包含子序列seqidno.4、5和6中的任一个或全部。包含子序列seqidno.69和子序列seqidno.4的3-酮酰基-辅酶a合酶可包含子序列seqidno.52或子序列seqidno.53。包含子序列seqidno.69、子序列seqidno.5和子序列seqidno.6的3-酮酰基-辅酶a合酶可包含子序列seqidno.46。包含子序列seqidno.69、4、5和6的3-酮酰基-辅酶a合酶可包含子序列seqidno.70。包含子序列seqidno.69的3-酮酰基-辅酶a合酶可包含子序列seqidno.48。包含子序列seqidno.69的3-酮酰基-辅酶a合酶可包含子序列seqidno.71，或者包含与子序列seqidno.71具有至少50％、至少75％、至少80％、至少90％或至少95％同一性的子序列，条件是3-酮酰基-辅酶a的与seqidno.71的氨基酸残基271对齐的氨基酸残基是异亮氨酸。这种3-酮酰基-辅酶a合酶可具有seqidno.115，或者与子序列seqidno.115具有至少50％、至少75％、至少80％、至少90％或至少95％同一性，条件是3-酮酰基-辅酶a的与seqidno.115的氨基酸残基271对齐的氨基酸残基是异亮氨酸。非天然脂肪酸合成途径中的其它反应步骤酰基-辅酶a与丙二酰基-辅酶a的反应产生3-酮酰基-辅酶a化合物，所述3-酮酰基-辅酶a化合物必须被还原为相应的酰基化合物，然后它才能与丙二酰基-辅酶a的另一个分子缩合以延伸链。还原以三个步骤发生，第一步骤是将3-酮酰基还原为相应的3-羟基酰基。第二个反应是脱水为相应的3-烯酰基酰基化合物，所述3-烯酰基酰基化合物在第三步骤中还原为相应的酰基-辅酶a。第一反应步骤由酮基-辅酶a还原酶(kcr)酶(ec1.1.1.35)酶促催化。第二步骤由3-羟基-酰基-辅酶a脱水酶(3hdh)酶(ec4.2.1.17)酶促催化。一些双功能酶催化第一步骤和第二步骤反应两者(ec1.1.1.35和ec4.2.1.55)。第三反应步骤由烯酰基-辅酶a还原酶(ecr)酶(ec1.1.1.32)酶促催化。因此，经遗传修饰的细胞优选地还包含以下中的至少一种：(1)编码kcr酶的异源kcr基因；(2)编码3hdh酶的异源3hdh基因；(3)编码双功能酶的异源基因，所述双功能酶催化第一和第二反应步骤两者(ec1.1.1.35和4.1.2.55)；以及(4)编码ecr酶的异源ecr基因。优选地，所述经遗传修饰的细胞含有至少(1)、(2)和(4)或至少(3)和(4)。在每种情况下，基因优选地处于宿主细胞中有活性的启动子和/或终止子序列的控制之下。kcr酶可以是，例如，由铜绿假单胞菌(p.aeruginosa)pafadb基因编码的和/或具有与seq.id.no.103具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的kcr酶；由铜绿假单胞菌fadg基因编码的和/或具有与seq.id.no.102具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的kcr酶；由拜氏梭菌(c.beijerinckii)hbd基因编码的和/或具有与seq.id.no.101具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的kcr酶；以及如在wo2015/010103中描述的其它kcr酶。3hdh酶可以是，例如，由丙酮丁醇梭菌(c.acetobutylicum)crt(短链烯酰基-辅酶a水合酶)基因编码的和/或具有与seq.id.no.99具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的3hdh酶；由恶臭假单胞菌(p.putida)ech(烯酰基-辅酶a水合酶/醛缩酶)基因编码的和/或具有与seq.id.no.100具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的3hdh酶；和如在wo2015/010103中描述的其它3hdh酶。催化第一和第二反应步骤的合适的双功能酶包括，例如，由大肠杆菌fadb基因编码的和/或具有与seq.id.no.98具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的双功能酶；由褐家鼠(r.novegicus)ech2基因编码的双功能酶；以及如在wo2015/010103中描述的其它双功能酶。合适的ecr酶包括，例如，由齿垢密螺旋体(t.denticola)ter基因编码的和/或具有与seq.id.no.84具有至少80％、至少90％、至少95％、至少99％或100％同一性的氨基酸序列的ecr酶。在一些实施方案中，本发明的经遗传修饰的细胞还包含不同于上述经修饰的3-酮酰基-辅酶a合酶的至少一种异源3-酮丁酰基-辅酶a合酶基因，所述至少一种异源3-酮丁酰基-辅酶a合酶基因编码3-酮丁酰基-辅酶a合酶。所述异源3-酮丁酰基-辅酶a合酶基因可编码与wo2015/10103的鉴定为seqidno.1-120的那些中的任一者具有至少80％、至少90％、至少95％、至少99％或100％同一性的3-酮丁酰基-辅酶a合酶。在一些实施方案中，所述异源3-酮丁酰基-辅酶a合酶基因是链霉菌属种cl190基因和/或编码与seqidno.83具有至少80％、至少90％、至少95％、至少99或100％同一性的npht7酶的基因。在一些实施方案中，本发明的经遗传修饰的细胞包含编码如wo2015/10103中所述的经修饰的npht7酶的至少一种基因。经修饰的npht7酶包含与seqidno.83具有至少70％但小于100％的氨基酸序列。经修饰的npht7酶可具有，例如，一个或多个选自由以下组成的组的氨基酸取代：h100l、i147t、f217v、y144l、v157f、g309s、g288s、氨基酸残基86-89的pdrp至hflq取代、i147f、i147m、i147q、i147s、i147c、1147e、i147n、i147w、i147d、i147r、i147p、i147l、v196g、i147g、i147h、i147k、i147v、i147a、i147y、f217g、f217a、f217l、f217i、f217m、f217t、f217p、f217s、f217e、f217l、f217v、f217w、s323a和s323v以及其任何两者或更多者的任何组合。在一些实施方案中，经修饰的npht7酶包含选自由i147v、i147s、i147t组成的组的至少一个氨基酸取代和选自h100l、f217v、s323a和s323v的至少一个另外的氨基酸取代。在一些实施方案中，经修饰的npht7酶对应于seqidno.82。在一些实施方案中，经修饰的npht7酶包含i147v、i147s或i147t氨基酸取代和s323a氨基酸取代(对应于seqidno.82，其中氨基酸100是h，氨基酸147是v、s或t，氨基酸217是f，并且氨基酸323是a)。在一些实施方案中，经修饰的npht7酶包含h100l取代、i147v、i147s或i147t氨基酸取代、f217v取代和s323a氨基酸取代(对应于seqidno.82，其中氨基酸残基100是l，氨基酸残基147是v、s或t，氨基酸残基217是v，并且氨基酸残基323是a)。在某些实施方案中，本发明的经遗传修饰的细胞包含(1)链霉菌属种cl190npht7基因和/或编码与seqidno.83具有至少80％、至少90％、至少95％、至少99％或100％同一性的npht7酶的基因；和(2经)修饰的npht7酶，其具有一个或多个选自由以下组成的组的氨基酸取代：h100l、i147t、f217v、y144l、v157f、g309s、g288s、氨基酸残基86-89的pdrp至hflq取代、i147f、i147m、i147q、i147s、i147c、1147e、i147n、i147w、i147d、i147r、i147p、i147l、v196g、i147g、i147h、i147k、i147v、i147a、i147y、f217g、f217a、f217l、f217i、f217m、f217t、f217p、f217s、f217e、f217l、f217v、f217w,s323aands323v以及其任何两者或更多者的任何组合。在优选的实施方案中，经遗传修饰的细胞包含编码具有seqidno.83的酶的基因和编码具有seqid.no.82的酶的另一种基因。在特别优选的实施方案中，经遗传修饰的细胞包含编码具有seqidno.83的酶的基因和编码具有seqid.no.82的酶的另一种基因，其中氨基酸残基100是h或l，氨基酸残基147是s、t或v，氨基酸残基271是f或v，并且氨基酸残基323是a。本发明的经遗传修饰的细胞产生一种或多种酶，所述一种或多种酶终止酰基延伸循环并产生具有所需链长的产物。这种终止酶可以是异源的，或者可以不是异源的。终止酶的选择可取决于所需产物是脂肪酸还是其衍生物如脂肪醇、脂肪醛、脂肪烯烃、脂肪酰胺、脂肪酯或脂肪烷烃。在一些实施方案中，本发明的细胞包含编码硫酯酶、如酰基辅酶a酯酶的异源硫酯酶基因，在这种情况下，产物将是脂肪酸。合适的硫酯酶包括wo2015/101013的表11中描述的那些。在一些实施方案中，本发明的细胞包含编码酯合酶的基因，在这种情况下，产物通常是脂肪酸酯。合适的酯合酶具有与以下中的任一者具有至少80％、至少90％、至少95％、至少99％或至少100％同一性的氨基酸序列：水油海杆菌(marinobacteraquacolei)maq1酶(wo2015/10103的seqidno.289)、盐晶嗜冷杆菌(psychrobactercryohaloentis)pcry1酶(wo2015/10103的seqidno290)、约氏红球菌(rhodococcusjostii)rjos1酶(wo2015/10103的seqidno291)、泊库岛食烷菌(alcanivoraxborkumensis)菌株sk2abork1酶(wo2015/10103的seqidno292)和济州岛霍氏菌(hahellachejuensis)hche基因(seqidno.104)。酯合酶可具有与济州岛霍氏菌hche酯合酶(seqidno.104)具有至少80％、至少90％、至少95％、至少99％或至少100％同一性的氨基酸序列。本发明的经遗传修饰的细胞还可包含编码以下中的一种或多种的一种或多种基因：脂肪酰基-辅酶a还原酶(醇或醛形成)、脂肪醛还原酶、酰基-acp还原酶、酰基辅酶a:acp酰基转移酶、酰基-辅酶a水解酶、羧酸还原酶、醛脱氢酶和/或酰基-acp还原酶。本发明的经遗传修饰的细胞还可包含(a)编码羧基转移酶亚基α酶(ec6.3.1.2)如大肠杆菌acca酶或与其具有至少80％、至少90％、至少95％或至少99％同一性的酶的一种或多种基因；(b)编码生物素羧基载体蛋白(ec6.4.1.2)如大肠杆菌accb酶或与其具有至少80％、至少90％、至少95％或至少99％同一性的酶的一种或多种基因；(c)编码生物素羧化酶亚基酶(ec6.3.4.14)如大肠杆菌accc酶或与其具有至少80％、至少90％、至少95％或至少99％同一性的酶的一种或多种基因；(d)羧基转移酶亚基β(ec6.4.1.2)，如大肠杆菌accd酶或与其具有至少80％、至少90％、至少95％或至少99％同一性的酶，或其任何两者或更多者的组合。在一些实施方案中，存在全部(a)–(d)。在一些实施方案中，本发明的经遗传修饰的细胞还包含一种或多种另外的遗传修饰，所述一种或多种另外的遗传修饰完全或部分抑制以下酶中的一种或多种的产生：甲基乙二醛合酶(ec4.2.3.3)，例如由大肠杆菌mgsa基因编码的甲基乙二醛合酶。乳酸脱氢酶(ec1.1.1.27)，例如由大肠杆菌ldha基因编码的乳酸脱氢酶。磷酸转乙酰酶(ec2.3.1.8)，例如由大肠杆菌pta基因编码的磷酸转乙酰酶。乙酸激酶(ec2.7.2.1)，例如由大肠杆菌acka基因编码的乙酸激酶。酰基-辅酶a合酶(ec6.2.1.3)，例如由大肠杆菌fadd基因编码的酰基-辅酶a合酶。丙酮酸甲酸裂解酶(ec2.3.1.54)，例如由大肠杆菌pflb基因编码的丙酮酸甲酸裂解酶。丙酮酸氧化酶(ec1.2.2.2)，例如由大肠杆菌poxb基因编码的丙酮酸氧化酶。融合乙醛-辅酶a脱氢酶(ec1.2.1.10)。触发因子(ec5.2.1.8)，例如由大肠杆菌tig基因编码的触发因子。限制性核酸内切酶(ec3.1.21.3)，例如由大肠杆菌hsdr514基因编码的限制性核酸内切酶。atodaeb操纵子。酰基-辅酶a硫酯酶(ec3.1.2.-)，例如由大肠杆菌tesb或ycia基因编码的酰基-辅酶a硫酯酶。酰基-辅酶a脱氢酶(ec1.3.8.7)，例如由大肠杆菌fade基因编码的酰基-辅酶a脱氢酶。3-酮酰基-辅酶a硫解酶(ec2.3.1.16)，例如由大肠杆菌fada基因编码的3-酮酰基-辅酶a硫解酶。l-核酮糖激酶(ec2.7.1.16)，例如由大肠杆菌arab基因编码的l-核酮糖激酶。l-核酮糖-5-磷酸-4-差向异构酶(ec5.1.3.4)，例如由大肠杆菌arad基因编码的l-核酮糖-5-磷酸-4-差向异构酶。β-d-半乳糖苷酶(ec3.2.1.23)，例如由大肠杆菌lacz基因编码的β-d-半乳糖苷酶。λ相溶素原。鼠李糖-1-磷酸醛缩酶(ec4.1.2.19)，例如由大肠杆菌rhad基因编码的鼠李糖-1-磷酸醛缩酶。鼠李糖激酶(ec2.7.1.5)，例如由大肠杆菌rhab基因编码的鼠李糖激酶。f交配因子。rph-1基因。其它遗传修饰可存在于细胞中，包括wo2015/10103中描述的那些中的任一种。任何异源基因可与在宿主菌株中有功能的启动子和/或终止子序列可操作地连接。启动子可以是仅在某些条件下起作用的诱导型启动子。例如，低磷酸盐诱导型启动子如野生型大肠杆菌phoe基因(pphoe)启动子)的启动子是3-酮酰基合酶基因的有用启动子。这种启动子在低磷酸盐环境中具有活性。因此，其中本发明的3-酮酰基合酶基因处于大肠杆菌phoe启动子或另一种低磷酸盐诱导型启动子控制下的微生物可在含有不超过25mm磷酸盐、尤其是不超过20mm、不超过2mm、不超过1mm、不超过0.5mm或不超过0.25mm磷酸盐的发酵培养基中培养)。任何异源基因可整合到宿主菌株的基因组中和/或存在于一种或多种质粒中。如果整合到基因组中，则可将异源基因插入在靶向或随机位置处。本领域中已知的转化方法如电穿孔和化学方法(包括氯化钙和/或乙酸锂方法)是合适的。合适的转化方法的实例描述于例如molecularcloning:alaboratorymanual,第4版springharborpress2012中。通常，不需要特殊的转化方法来产生本发明的经遗传修饰的细胞。天然基因的缺失和/或破坏可通过转化方法、通过诱变和/或通过强制进化方法来进行。在诱变方法中，在足以实现细胞的高杀伤率(60％-99.9％、优选90％-99.9％)的条件下，将细胞暴露于紫外线辐射或诱变物质。然后将存活的细胞接种并选择或筛选具有缺失或破坏的代谢活性的细胞。可通过pcr或southern分析方法确认所需天然基因的破坏或缺失。本文描述的经遗传修饰的细胞用于产生具有直链烷基的化合物。使细胞在使得它们产生此类化合物的条件下生长，并回收所述化合物。当宿主细胞是植物细胞时，可使植物生长并且可从所述植物或其任何部分(如根、茎、叶、花、种子、种子荚等)中回收具有直链烷基的化合物，其中所述化合物在植物的生长期间累积。本发明的单细胞和其它微细胞可用于培养过程以产生此类化合物。通常通过形成培养基来进行培养，所述培养基包含至少一种能够被细胞代谢以产生特定细胞可能需要的产物化合物和营养物的碳源。营养物可包括，例如，至少一种氮源(如酵母提取物、蛋白胨、胰蛋白胨、大豆粉、玉米浆或酪蛋白)、至少一种磷源、一种或多种维生素(如生物素、维生素b12和维生素b12的衍生物、硫胺素、泛酸)、一种或多种痕量金属等。发酵培养基还可含有另外材料，如消泡剂、杀生物剂、缓冲剂等。在一些情况下，如脂肪酸酯的生产，培养基还可包含与直链化合物反应以产生所需产物的试剂。在脂肪酸酯的具体情况下，例如培养基优选地含有烷醇，如甲醇、乙醇或c3-c8烷醇。烷醇反应以产生相应的酯。细胞可表达天然或异源酯合酶或其它合适的酶以催化这种反应。通常，培养基接种有本发明的细胞，并在培养基中培养接种物，以使得细胞密度增加至适于生产的细胞密度。然后将培养基保持在足以使细胞产生所需产物的条件下。合适的培养条件当然将取决于特定宿主菌株的需要。培养基的温度可以是例如20℃至70℃，对于大多数细胞，25℃至40℃的温度是优选的。培养基的ph可以是，例如，2.0至10.0、3.0至9.0或6.0至ph8.5。预期本发明的实施方案可使用分批、补料分批或连续过程来实施，并且任何已知的生物生产模式将是合适的。培养可根据需要在需氧、微需氧或厌氧条件下进行，或者可由特定细胞耐受。通常，不需要特殊的培养设备来进行发酵。所述设备可包括，例如，适于容纳细胞和培养基的罐；用于将内容物从培养罐排出到提取和/或分离容器的管线；以及适于从细胞培养废物中除去化学产物的提取和/或分离容器。碳源是一种或多种含碳化合物，其可被本发明的细胞代谢为碳源。合适的碳源的实例包括糖，如葡萄糖、蔗糖、果糖、乳糖；c-5糖，如木糖和阿拉伯糖；甘油和多糖，如淀粉和纤维素。其它合适的碳源包括如可通过预处理和糖化的方法从纤维素和木质纤维素生物质获得的可发酵的糖，如例如在美国专利公布号2007/0031918a1中所描述；半纤维素、木质素、淀粉、低聚糖和/或单糖。其它合适的碳源包括高果糖玉米糖浆、奶酪乳清渗透液、玉米浆、甜菜糖蜜和大麦芽。其它合适的碳源还包括二氧化碳、一氧化碳、甲醇、甲胺和葡萄糖胺。可继续培养过程，直至所需产物的滴度达到每升培养基至少0.01、至少0.05、至少0.1、至少0.25、至少0.5或至少1g(g/l)。发酵过程可持续直到滴度达到例如高达40、高达45、高达50、高达80、高达100或高达120g/l。比生产率可以是，例如，基于干重每小时每克细胞0.01和0.60克的所需产物(g化学产物/gdcw-hr)。所达到的体积生产率可以是每小时每升至少0.005g的所需产物(g/l-hr)、至少0.01g/l-hr、至少0.1g/l-hr或至少0.5g/l-hr，并且可高达例如10g/l-hr、高达5g/l-hr或高达1g/l-hr。在一些实施方案中，如在24小时发酵(培养)期内测量的比生产率可以是每克dcw的细胞大于约0.01、0.05、0.10、0.20、0.5、1.0、2.0、3.0、4.0、5.0、6.0、7.0、8.0、9.0、10.0、11.0或12.0克的化学产物(基于在24小时时期结束时的最终dcw)。提供以下实施例以说明本发明，但不意图限制其范围。除非另外指明，否则所有份数和百分比都是按重量计。实施例如所指示，以下宿主大肠杆菌菌株用于以下实施例中。宿主菌株1是指定为bw25113的大肠杆菌菌株的突变体，其可从大肠杆菌遗传菌株中心(cgsc#7636；dept.ofmolecular,cellular,anddevelopmentalbiology,yaleuniversity,newhaven,connecticut)获得，具有以下另外的遗传修饰：宿主菌株2是bw25113大肠杆菌菌株的突变体，具有以下另外的遗传修饰：宿主菌株3是bw25113大肠杆菌菌株的突变体，具有以下遗传修饰：3-酮酰基-辅酶a合酶基因和突变体的产生基于各种野生型3-酮酰基-辅酶a合酶基因的公开序列信息合成3-酮酰基-辅酶a合酶基因。通过寡核苷酸定向诱变产生合成的3-酮酰基-辅酶a合酶基因的位点特异性突变体。野生型基因的来源和本文中用于它们中的每一个的简写名称以及由野生型基因产生的天然酶的氨基酸序列如下：源物种名称所编码的酶(野生型菌株)申氏不动杆菌cip107287aschseqidno.8申氏不动杆菌niph900asch-2seqidno.86约氏不动杆菌sh046ajoh-2seqidno.35鲁氏不动杆菌sh145alwoseqidno.88不动杆菌属种niph713anip71seqidno.89施氏假单胞菌atcc17588pstuseqidno.90土壤异希瓦氏菌bl06aagrseqidno.91在每种情况下，将3-酮酰基-辅酶a合酶基因与编码含有6个组氨酸残基和蛋白酶识别位点的蛋白质片段的dna序列融合，并且并入具有cole1复制起点和卡那霉素抗性标记的pet质粒中。由野生型基因编码的氨基酸残基的突变在本文中用野生型菌株的简写命名，在括号中后接由表示天然酶中命名氨基酸残基的第一个字母、表示所述氨基酸残基在所述天然酶中的位置的1-、2-或3-位数数字以及命名突变酶中所述位置中的氨基酸残基的最后一个字母组成的3-、4-或5字符代码。单字母名称是iupac氨基酸缩写，如例如在eur.j.biochem.138:9-37(1984)中所报告。例如，名称“asch(t184i)”表示野生型申氏不动杆菌cip107287酶中氨基酸残基位置184处的苏氨酸(t)已被异亮氨酸(i)替代。多重突变的3-酮酰基-辅酶a合酶基因的产生。使用易错pcr作为诱变方法，从野生型或突变的(亲本)3-酮酰基-辅酶a合酶基因制备多重突变基因。使用具有seqidno.94和seqidno.95的引物以及econotaqdna聚合酶(lucigen)与热循环程序：94℃2分钟、30x[94℃20秒、55℃20秒、72℃72描]、72℃10分钟、4℃保持来进行3-酮酰基-辅酶a合酶基因的易错pcr。此外，易错pcr反应含有50、100、150或200μmmncl2。用dnacleanandconcentrator试剂盒(zymoresearch)纯化pcr片段，用dpni在37℃下消化1小时，并再次纯化。使用2xhifiassemblymastermix(2倍摩尔过量的插入物与质粒)组装质粒和插入物，并在50℃孵育1小时。由多重突变基因产生的基因的氨基酸序列用如上所述的简写命名，其中突变依次列出。例如，“asch(t184i,s328v)”表示野生型申氏不动杆菌cip107287酶中氨基酸残基位置184处的苏氨酸(t)已被异亮氨酸(i)替代，并且位置328处的丝氨酸已被缬氨酸替代。突变型大肠杆菌菌株的产生使用标准电穿孔方法制备突变型大肠杆菌菌株。在每种情况下，宿主菌株用“1型”质粒和“2型”质粒转化，如下所述。1型质粒是含有p15a复制起点和氯霉素抗性标记的pacyc质粒。以下实施例中使用的1型质粒是：1a型：这种质粒包含编码具有h100l、i147s、f217v和s323a突变的3-酮酰基-辅酶a合酶的突变的链霉菌属种npht7基因(“lsva”npht7突变体，seqidno.82)、大肠杆菌双功能3-羟基酰基-辅酶a脱氢酶/脱水酶(fadb)基因和齿垢密螺旋体烯酰基-辅酶a(ter)基因盒，所述基因都在天然大肠杆菌pstsih启动子和天然大肠杆菌终止子下。这种质粒还含有在大肠杆菌phoe启动子下的与编码含有6个组氨酸残基和蛋白酶识别位点的蛋白质片段的dna序列融合的济州岛霍氏菌酯合酶基因，和包含具有大肠杆菌tpia启动子的融合的大肠杆菌acca和accd基因的acc(乙酰基-辅酶a羧化酶)盒以及包含在大肠杆菌rpia启动子下的大肠杆菌accb和大肠杆菌accc基因的盒。1b型：这种质粒包含在天然大肠杆菌pstsih启动子和天然大肠杆菌终止子下的编码具有i147s和f217v修饰的npht7酶的链霉菌属种基因(“sv”npht7突变体，seqidno.96)和齿垢密螺旋体烯酰基-辅酶a(ter)基因盒。所述质粒还含有在天然大肠杆菌pstsih启动子下的大肠杆菌双功能3-羟基酰基-辅酶a脱氢酶/脱水酶(fadb)基因；和在大肠杆菌phoe启动子下的与编码含有6个组氨酸残基和蛋白酶识别位点的蛋白质片段的dna序列融合的济州岛霍氏菌酯合酶基因。2a型质粒包含cole1复制起点、卡那霉素抗性标记以及在大肠杆菌启动子和大肠杆菌终止子下的待评价的3-酮酰基-辅酶a合酶基因(与编码含有6个组氨酸残基和蛋白酶识别位点的n-末端蛋白质片段的dna序列融合，除非另外说明)。大肠杆菌启动子是天然psts基因的启动子(ppstsih启动子)或天然phoe基因的启动子(pphoe启动子)，其中后者是低磷酸诱导型。3-酮酰基-辅酶a合酶和3-酮酰基-辅酶a合酶基因的启动子如下文的具体实施例中所示。质粒2b包含cole1复制起点、卡那霉素抗性标记以及在ppstsih(seqidno.118)或pphoe启动子(seqidno.117)和大肠杆菌终止子下的待评价的3-酮酰基-辅酶a合酶基因(在一些情况下没有his标签)。3-酮酰基-辅酶a合酶及其基因的启动子如下文的具体实施例中所示。小规模发酵方法将含有盐、葡萄糖、nh4cl并补充有维生素、酵母提取物、35μg/ml卡那霉素和20μg/ml氯霉素的合成培养基的培养物接种待测试的菌株并在30℃下生长过夜。测定这种培养物的1:10稀释液的od600，并且离心对应于8个od单位的原始培养物的体积，并丢弃上清液。将沉淀的细胞充分重悬于4ml新鲜培养基中，所述培养基含有30g/l葡萄糖、0.158mm磷酸盐(低磷酸盐培养基)、1％(v/v)甲醇(以产生脂肪酸甲酯)或乙醇(以产生脂肪酸乙酯)、氯霉素和如上的卡那霉素，并且将1-ml等分试样分配到含有64μl十七烷或十四烷酸甲酯的一式三份的16-mm玻璃管中。这代表有限的磷酸盐培养基，其促进低磷酸盐诱导型启动子如大肠杆菌pphoe启动子(seqidno.117)的活性。将管在30℃、250rpm下孵育4小时。然后将孵育温度升至37℃并继续孵育另外20小时。用甲基叔丁基醚萃取整个培养物，并通过气相色谱分析萃取物的脂肪酸酯。用于实施例80-116的小规模发酵方法将含有盐、葡萄糖、nh4cl并补充有维生素、酵母提取物、35μg/ml卡那霉素和20μg/ml氯霉素的合成培养基的培养物接种待测试的菌株并在32℃下生长过夜。测定这种培养物的1:10稀释液的od600，以将种子2烧瓶接种至0.3的最终od600。种子2烧瓶含有25-30ml合成培养基，所述合成培养基含有盐、葡萄糖、nh4cl并补充有维生素、酵母提取物、35μg/ml卡那霉素和20μg/ml氯霉素、0％-2％甲醇。将种子2烧瓶在32℃下孵育6-7小时。测定这种培养物的1:10稀释液的od600，以将生产烧瓶接种至0.01-0.025的最终od600。生产烧瓶含有25ml合成培养基，所述合成培养基含有盐、葡萄糖、nh4cl并补充有维生素、酵母提取物、35μg/ml卡那霉素和20μg/ml氯霉素、1.25-2.5mm磷酸盐、0％-2％甲醇、3-6g/l葡萄糖、15-40g/l甘油。这代表有限的磷酸盐培养基，其促进低磷酸盐诱导型启动子如大肠杆菌pphoe启动子(seqidno.117)的活性。将生产烧瓶在32℃下孵育。当磷酸盐耗尽时，进行以下添加：2ml的肉豆蔻酸甲酯、1ml的12.5％tween80、1-1.25ml的50％甘油。在生产测定开始时或在磷酸盐耗尽后立即添加甲醇(0-0.5ml)。在磷酸盐耗尽后可添加额外的甲醇以补偿甲醇蒸发。将烧瓶在35℃下再孵育24小时。取得24小时时的样品并用在mtbe(甲基叔丁基醚)中的0.1％hcl萃取，并通过气相色谱分析萃取物的脂肪酸酯。用于实施例117-148的小规模发酵方法将含有盐、葡萄糖、nh4cl并补充有维生素、酵母提取物、35μg/ml卡那霉素和20μg/ml氯霉素的合成培养基的浅96孔板接种待测试的菌株并在30℃下生长过夜。来自浅96孔板的培养物用于以每孔2-3μl接种深孔生产板。生产板的每个孔含有400μl合成培养基，所述合成培养基含有盐、葡萄糖、nh4cl并补充有维生素、35μg/ml卡那霉素和20μg/ml氯霉素、1.25mm磷酸盐、2％甲醇、3g/l葡萄糖、30g/l甘油。这代表有限的磷酸盐培养基，其促进低磷酸盐诱导型启动子如大肠杆菌pphoe启动子(seqidno.117)的活性。将生产板在32℃下孵育。当磷酸盐耗尽时，向每个孔中添加26μl肉豆蔻酸甲酯。将板在35℃下再孵育20小时。取得20小时时的样品并用乙腈稀释，并通过气相色谱分析脂肪酸酯。实施例1-15和对比样品a-c-asch(t184i)变体突变型大肠杆菌菌株实施例1和2含有编码3-酮酰基-辅酶a合酶asch(t184i)的修饰的asch基因(seqidno.9)。对比样品a和b含有野生型基因(编码seqidno.8)。菌株构建的细节如下：*不是本发明的实施例。使用上述小规模方法培养实施例1和2以及对比样品中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示。对c8和c10脂肪酸酯的选择性在相应的“％”栏下表示；这些被计算为c8或c10脂肪酸酯相对于总脂肪酸酯产量的滴度。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。*不是本发明的实施例。**在此表和后续表中的“wt”表示野生型酶。野生型asch酶产生约82％的c10脂肪酸甲酯，具有最少量的c8酯。通过将位置184的苏氨酸改变为异亮氨酸，产生从几乎唯一的c10脂肪酸酯转变为主要是c8脂肪酸酯，同时也观察到对c6脂肪酸酯的一些增加的选择性。突变型菌株实施例1和2可用于产生富含c8酯的脂肪酸酯的混合物。突变型大肠杆菌菌株实施例3-5类似地含有编码突变的3-酮酰基-辅酶a合酶(asch(t184l)、asch(t184m)或asch(t184v))的经修饰的asch基因。对比样品c再次含有野生型基因。菌株构建的细节如下：*不是本发明的实施例。使用上述小规模方法培养实施例3-5以及对比样品c中的每一个以产生脂肪酸乙酯。所产生的总脂肪酸乙酯(faee)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总faee产值中。*不是本发明的实施例。如上文在对比样品a和b情况下所看出，野生型asch酶主要导致c10脂肪酸酯产生。t184l、t184m和t184v变异都将产生从c10脂肪酸酯转向主要是c6和c8脂肪酸酯。实施例4中的t184m变异将c10脂肪酸酯产生降低至小于2％。对于t184v变异，对c8脂肪酸酯的选择性几乎为50％。突变型大肠杆菌菌株实施例6-15类似地含有突变的asch基因，所述突变的asch基因编码具有多个突变的突变的3-酮酰基-辅酶a合酶，如下表中所示。菌株构建的细节如下：使用上述小规模方法培养实施例6-15中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。如此数据所示，含有t184突变的多重突变酶都将产生从c10脂肪酸酯转向主要是c8脂肪酸酯。具有s328g变异的突变酶在这种评价中对c8脂肪酸酯尤其具有选择性，其中选择性接近80％。实施例16-18和对比样品d–asch-2变体突变型大肠杆菌菌株实施例16-18含有编码3-酮酰基-辅酶a合酶(asch-2(t184m)、asch-2(t184v)和asch-2(t184i)，分别seqidno.22-24)的经修饰的asch-2基因。对比样品d含有野生型基因(编码seqidno.86)。菌株构建的细节如下：使用上述小规模方法培养实施例16-18以及对比样品d中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总faee产值中。*不是本发明的实施例。野生型asch-2基因几乎完全产生c10脂肪酸酯。t184变异都使产生从c10脂肪酸酯转向c6和c8脂肪酸酯。通过对asch-2基因的这些修饰，对c8脂肪酸酯的选择性从0增加至约30％-45％。实施例19-22和对比样品e–alwo变体突变型大肠杆菌菌株实施例19-22含有编码突变的3-酮酰基-辅酶a合酶(alwo(t184l)、alwo(t184m)、alwo(t184v)和alwo(t184i)，分别seqidno.26-29)的经修饰的alwo基因。对比样品e含有野生型基因(编码seqidno.88)。菌株构建的细节如下：使用上述小规模方法培养实施例19-22以及对比样品e中的每一个以产生脂肪酸乙酯。所产生的总脂肪酸乙酯(faee)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总faee产值中。*不是本发明的实施例。alwo酶中的t184变异的影响类似于在asch和asch-2突变中所观察到的影响。尽管野生型alwo酶产生超过90％的c10脂肪酸酯且没有c8脂肪酸酯，但t184l、m、v和i变异都使产生转向c6和c8脂肪酸酯。在此方面，t184m和t184i变异特别有效，在那些情况中的每种下c10脂肪酸酯选择性被降低至20％以下，并且在t184i情况下c8脂肪酸酯选择性超过60％。实施例23-26和对比样品f–ajoh-2变体突变型大肠杆菌菌株实施例23-26含有编码3-酮酰基-辅酶a合酶(ajoh-2(t184l)、ajoh-2(t184m)、ajoh-2(t184v)和ajoh-2(t184i)，分别seqidno.36-39)的经修饰的ajoh-2基因。对比样品f含有野生型基因(编码seqidno.35)。菌株构建的细节如下：使用上述小规模方法培养实施例23-26以及对比样品f中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总faee产值中。*不是本发明的实施例。在ajoh-2基因修饰情况下可观察到相同的一般模式。野生型基因对c10脂肪酸酯产生的高选择性转向c6和c8脂肪酸酯产生。在此方面，t184m和t184i变异特别有效。与t184l、m和i变异相比，t184v变异使得总体生产率大幅提高。实施例27-29和对比样品g–anip71变体突变型大肠杆菌菌株实施例27-29含有编码3-酮酰基-辅酶a合酶(anip71(t184m)、anip71(t184v)和anip71(t184i)，分别seqidno.32、34和31)的经修饰的anip71基因。对比样品g含有野生型基因(编码seqidno.89)。菌株构建的细节如下：使用上述小规模方法培养实施例27-29以及对比样品g中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。*不是本发明的实施例。如之前，t184变异都使产生从c10脂肪酸酯转向c8脂肪酸酯，其中t184m和t184i变异特别有效。与t184m和t184i变异相比，t184v变异使得总体生产率大幅提高。t184i变异对c8脂肪酸酯表现出最高的选择性。实施例30-31和对比样品h–pstu变体突变型大肠杆菌菌株实施例30-31含有编码3-酮酰基-辅酶a合酶(分别pstu(c186m)和pstu(c186i)，seqidno.40，其中位置186分别是m和i)的经修饰的pstu基因。对比样品h含有野生型基因(编码seqidno.90)。菌株构建的细节如下：使用上述小规模方法培养实施例30-31以及对比样品h中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。*不是本发明的实施例。野生型pstu酶几乎完全产生c10脂肪酸酯。c186m和i变异两者均使产生转向c6和c8脂肪酸酯，其中对c8脂肪酸酯的选择性为约40％-60％。实施例32-61和对比样品i–aagr变体突变型大肠杆菌菌株实施例32-61含有编码3-酮酰基-辅酶a合酶的经修饰的aagr基因，如下表中所示。对比样品i含有野生型基因(编码seqidno.91)。菌株构建的细节如下：在上述小规模方法中培养实施例32、35-42、44和50-61以及对比样品i中的每一个以产生脂肪酸乙酯。所产生的总脂肪酸乙酯(faee)以及c8和c10脂肪酸酯的量如下文在下表中所示，对c8、c10和c12脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总faee产值中。“c12产生”栏下的“少量”意味着表中未具体说明的大多数faee产生是c6乙酯，其中产生的c12脂肪酸酯少于5％。*不是本发明的实施例。在此评价中，野生型aagr酶产生18％的c12脂肪酸酯。野生型aagr的替代减少c12脂肪酸酯产生，在大多数情况下有利于对c8和/或c10脂肪酸酯的更高选择性。使用上述小规模方法培养实施例32-34和43-49中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c8和c10脂肪酸酯的量如下表中所示，对c8、c10和c12脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。“c12产生”栏下的“少量”意味着表中未说明的大多数fame产生是c6甲酯，其中产生的c12脂肪酸酯少于5％。如前两个表中的数据所示，野生型aagr酶产生显著比例(18％)的c12脂肪酸酯。a186i修饰本身或伴随另外的修饰在faee和fame产生两者中减少c12脂肪酸酯产生，有利于c8和/或c10脂肪酸酯，并且在一些情况下还有利于c6脂肪酸酯。实施例62-71和对比样品j和k—asch变体突变型大肠杆菌菌株实施例62-71含有编码3-酮酰基-辅酶a合酶的经修饰的asch基因，如下表中所示。对比样品j和k含有野生型基因(编码seqidno.8)。仅在实施例62-64和对比样品j中，突变型asch基因与编码含有6个组氨酸残基和蛋白酶识别位点的蛋白质片段的dna序列融合。菌株构建的细节如下：使用上述小规模方法培养62-71以及对比样品j和k中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。如此数据所示，与野生型asch酶相比，k278r、v296a、v317a、m271i和m178l突变都使得细胞的总生产率提高。在这些实施例中，生产率提高了多达36％。与对比j和实施例62-64相比，对比k和实施例65-71的通常较高的生产率据信可归因于在低磷酸盐诱导型启动子控制下具有3-酮酰基合酶连同低磷酸盐培养基的选择的组合。实施例72-74—asch(t184i)变体突变型大肠杆菌菌株实施例72-74类似地含有突变的asch基因，所述突变的asch基因编码具有多个突变的突变的3-酮酰基-辅酶a合酶，如下表中所示。菌株构建的细节如下：使用上述小规模方法培养实施例72-74中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c6、c8和c10脂肪酸酯的量如下表中所示，对c8和c10脂肪酸酯的选择性也如下表中所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。实施例75-77通过使用前述电穿孔方法用“1型”质粒和“2型”质粒转化大肠杆菌菌株bw25113来制备大肠杆菌突变体。菌株构建的细节如下：当使用小规模发酵方法评价时，所有实施例75-77对c6-c10脂肪酸酯表现出良好的选择性。实施例78酿酒酵母菌株imx581(mans,r.,h.m.vanrossum,等人(2015).crispr/cas9:amoleculartoolforsimultaneousintroductionofmultiplegeneticmodificationsinsaccharomycescerevisiae.femsyeastres15(2))具有整合在其染色体中的cas9核酸酶，以使得它可用作使用crispr技术操纵基因组的宿主菌株(us20140068797a1)。引导rna(grna)由pmel或pros系列质粒表达。使用这种技术将非天然脂肪酸和脂肪酸酯途径的基因整合在imx581的染色体中。grna序列使用yeastriction在线工具设计(robertmans,harmenm.vanrossum,melaniewijsman,antoonbackx,nielsg.a.kuijpers,marcelvandenbroek,pascaledaran-lapujade,jackt.pronk,antoniusj.a.vanmaris,jean-marcg.daran(2015)crispr/cas9:amolecularswissarmyknifeforsimultaneousintroductionofmultiplegeneticmodificationsinsaccharomycescerevisiae.femsyeastresearch16)。使用具有50bp同源性的互补引物将grna序列引入pmel质粒中并进行page纯化。将引物溶解在蒸馏水中至100μm的最终浓度，将引物以1:1摩尔比混合，并将混合物加热至95℃持续5分钟且通过冷却至室温进行退火。将引物与作为模板的pmel10混合，并使用q5highfidelity2xmastermix(newenglandbiolabs(ipswich,mass.)扩增混合物。将pcr产物用dpni消化30分钟，并将pcr产物在琼脂糖凝胶上纯化。mans等人(同上)中描述了同时整合和缺失的方案。使用所述方案，将编码下表中列出的蛋白质的基因整合到如下文所列的酿酒酵母染色体的基因座中。用于表达所述基因的终止子和启动子也列于表中。使工程改造的酵母在250ml摇瓶中在30℃在25ml补充有10g/l葡萄糖作为碳源的合成限定培养基中生长。将烧瓶以200rpm振荡24小时。在上清液中测量脂肪酸或脂肪酸甲酯酸。实施例79产油酵母解脂耶氏酵母菌株lgams(7)1(papanikolaous.,andaggelisg.,bioresour.technol.82(1):43-9(2002))。crispr/cas9：一种用于在解脂耶氏酵母中同时引入多种遗传修饰的分子工具。将宿主用整合在其染色体中的cas9核酸酶工程改造，以使得它可用作使用crispr技术操纵基因组的宿主菌株(us20140068797a1)。引导rna(grna)由pmel或pros系列质粒表达。使用这种技术将非天然脂肪酸和脂肪酸酯途径的基因整合在解脂耶氏酵母的染色体中。grna序列使用yeastriction在线工具设计(robertmans,harmenm.vanrossum,melaniewijsman,antoonbackx,nielsg.a.kuijpers,marcelvandenbroek,pascaledaran-lapujade,jackt.pronk,antoniusj.a.vanmaris,jean-marcg.daran(2015)crispr/cas9:amolecularswissarmyknifeforsimultaneousintroductionofmultiplegeneticmodificationsinsaccharomycescerevisiae.femsyeastresearch16)。使用具有50bp同源性的互补引物将grna序列引入pmel质粒中并进行page纯化。将引物溶解在蒸馏水中至100μm的最终浓度，将引物以1:1摩尔比混合，并将混合物加热至95℃持续5分钟且通过冷却至室温进行退火。将引物与作为模板的pmel10混合，并使用q5highfidelity2xmastermix(newenglandbiolabs(ipswich,mass.)扩增混合物。将pcr产物用dpni消化30分钟，并将pcr产物在琼脂糖凝胶上纯化。mans等人(同上)中描述了同时整合和缺失的方案。使用所述方案，将编码下表中列出的蛋白质的基因整合到解脂耶氏酵母染色体的基因座中。用于表达所述基因的终止子和启动子的实例也列于表中。使工程改造的酵母在250ml摇瓶中在30℃在25ml补充有10g/l葡萄糖作为碳源的合成限定培养基中生长。将烧瓶以200rpm振荡24小时。在上清液中测量脂肪酸或脂肪酸甲酯酸。实施例80-96—asch(t184i)变体具有seqidno.20的突变型3-酮酰基-辅酶a合酶被选择作为通过另外的突变进一步改善的有希望的候选物。通过将具有seqidno.20的突变型3-酮酰基-辅酶a合酶引入到大肠杆菌宿主菌株3中来产生本发明的对照a。本发明实施例充当用于比较另外的突变体实施例80-96的基础。突变型大肠杆菌菌株实施例80-96含有突变的asch基因，所述突变的asch基因编码具有与seqidno.20相同的突变以及一种或多种另外的突变的突变的3-酮酰基-辅酶a合酶。与野生型3-酮酰基-辅酶a合酶(seqidno.8)不同的所有突变在下表中示出。菌株构建的细节如下：使用上述摇瓶方法培养实施例80-96中的每一个以产生脂肪酸甲酯。测量c8和c10脂肪酸酯的量。如所示记录每个实施例增加c8和/或c10脂肪酸酯的产生的能力。如所示记录每个实施例增加对c8和/或c10的特异性的能力。*(+)＝相对于对照菌株增加**(+)＝相对于对照菌株增加；(-)＝相对于对照菌株无变化；(o)＝相对于对照菌株减少实施例97-99—asch(t184i)变体具有seqidno.20的突变型3-酮酰基-辅酶a合酶(除了出现在氨基酸30和317处的缬氨酸各自被丙氨酸替代外)被选择作为通过另外的突变进一步改善的候选物。通过将这种突变型3-酮酰基-辅酶a合酶引入到大肠杆菌宿主菌株2中来产生本发明的对照b。本发明实施例充当用于比较另外的突变体实施例97-99的基础。突变型大肠杆菌菌株实施例97-99含有突变的asch基因，所述突变的asch基因编码具有与本发明对照b的突变相同的突变以及一种或多种另外的突变的突变的3-酮酰基-辅酶a合酶。与野生型3-酮酰基-辅酶a合酶(seqidno.8)不同的所有突变在下表中示出。菌株构建的细节如下：使用上述摇瓶方法培养实施例97-99中的每一个以产生脂肪酸甲酯。测量c8和c10脂肪酸酯的量。如所示记录每个实施例增加c8和/或c10脂肪酸酯的产生的能力。如所示记录每个实施例增加对c8和/或c10的特异性的能力。*(+)＝相对于对照菌株增加**(+)＝相对于对照菌株增加；(-)＝相对于对照菌株无变化；(o)＝相对于对照菌株减少实施例100-116—asch(t184i)变体对应于seqidno.8的具有如下表所示的六种特定突变的突变的3-酮酰基-辅酶a合酶被选择作为通过另外的突变进一步改善的候选物。通过将这种突变型3-酮酰基-辅酶a合酶引入到大肠杆菌宿主菌株3中来产生本发明的对照c。本发明实施例充当用于比较另外的突变体实施例100-116的基础。突变型大肠杆菌菌株实施例100-116含有突变的asch基因，所述突变的asch基因编码具有与本发明对照c的突变相同的突变以及一种或多种另外的突变的突变的3-酮酰基-辅酶a合酶。与野生型3-酮酰基-辅酶a合酶(seqidno.8)不同的所有突变在下表中示出。菌株构建的细节如下：使用上述摇瓶方法培养实施例100-116中的每一个以产生脂肪酸甲酯。测量c8和c10脂肪酸酯的量。如所示记录每个实施例增加c8和/或c10脂肪酸酯的产生的能力。*(+)＝相对于对照菌株增加**(+)＝相对于对照菌株增加；(-)＝相对于对照菌株无变化；(o)＝相对于对照菌株减少实施例117-133—asch(g51)变体突变型大肠杆菌菌株实施例117-133类似地含有突变的asch基因，所述突变的asch基因编码具有多个突变的突变的3-酮酰基-辅酶a合酶，如下表中所示。菌株构建的细节如下：使用上述摇瓶方法培养实施例117-133中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c8和c10脂肪酸酯的量如下表所示，c8和c10脂肪酸的相对百分比也如下表所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。实施例134-148—asch(g51)变体对应于seqidno.8的具有如下表所示的七种特定突变的突变的3-酮酰基-辅酶a合酶被选择作为通过另外的突变进一步改善的候选物。通过将这种突变型3-酮酰基-辅酶a合酶引入到大肠杆菌宿主菌株3中来产生本发明的对照d。本发明实施例充当用于比较另外的突变体实施例134-148的基础。突变型大肠杆菌菌株实施例134-148含有突变的asch基因，所述突变的asch基因编码具有与本发明对照d的突变相同的突变以及一种或多种另外的突变的突变的3-酮酰基-辅酶a合酶。与野生型3-酮酰基-辅酶a合酶(seqidno.8)不同的所有突变在下表中示出。菌株构建的细节如下：使用上述摇瓶方法培养实施例134-148中的每一个以产生脂肪酸甲酯。所产生的总脂肪酸甲酯(fame)以及c8和c10脂肪酸酯的量如下表所示，c8和c10脂肪酸的相对百分比也如下表所示。高和低碳数脂肪酸酯的量未单独示出，但包括在所指示的总fame产值中。实施方案1.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括以下中的至少一者：a)与seqidno.1具有至少80％同一性的子序列，条件是与seqidno.1的氨基酸残基8对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸，并且氨基酸残基2是亮氨酸或甲硫氨酸；b)与seqidno.2至少具有80％同一性的子序列，条件是与seqidno.2的氨基酸残基6对齐的氨基酸残基是异亮氨酸或甲硫氨酸；以及c)与seqidno.3具有至少80％同一性的子序列，条件是与seqidno.3的氨基酸残基6对齐的氨基酸残基是异亮氨酸、甲硫氨酸、苏氨酸、半胱氨酸、缬氨酸、谷氨酰胺、苯丙氨酸、天冬氨酸、天冬酰胺或酪氨酸。2.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括以下中的至少一者：a)seqidno.1、b)seqidno.2和c)seqidno.3。3.如实施方案2所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.1。4.如实施方案1-3中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括以下中的至少一者：a)seqidno.4或seqidno.161、b)seqidno.5以及c)seqidno.6或seqidno.162。5.如实施方案4所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.44。6.如实施方案5所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括与seqidno.45具有至少85％同一性的子序列，条件是所述3-酮酰基-辅酶a合酶的与seqidno.5的氨基酸残基35对齐的氨基酸残基是亮氨酸、缬氨酸、异亮氨酸或甲硫氨酸。7.如实施方案5所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.45。8.如实施方案4-7中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.4或seqidno.161、seqidno.5以及seqidno.6或seqidno.162。9.如实施方案7所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.46。10.如实施方案8所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.47。11.如实施方案1-10中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.48。12.如实施方案3所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.49具有至少80％同一性。13.如实施方案3所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列具有seqidno.49。14.如实施方案1-13中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.8具有至少50％同一性。15.如实施方案14所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.8具有至少80％同一性。16.一种3-酮酰基-辅酶a合酶，其具有选自由以下组成的组的氨基酸序列：seqidno.9、seqidno.10、seqidno.11、seqidno.12、seqidno.13、seqidno.14、seqidno.15、seqidno.16、seqidno.17、seqidno.18、seqidno.19、seqidno.20、seqidno.21、seqidno.22、seqidno.23、seqidno.24、seqidno.25、seqidno.26、seqidno.27、seqidno.28、seqidno.29、seqidno.30、seqidno.31、seqidno.32、seqidno.33、seqidno.34、seqidno.36、seqidno.37、seqidno.38、seqidno.39、seqidno.92、seqidno.93、seqidno121-157中的任一个或seqidno.172-204中的任一个。17.如实施方案2所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.2。18.如实施方案17所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括以下中的至少一者：a)seqidno.4或seqidno.161、b)seqidno.5以及c)seqidno.6或seqidno.162。19.如实施方案18所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.40具有至少50％同一性。20.如实施方案19所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.40具有至少80％同一性。21.如实施方案18所述的3-酮酰基-辅酶a合酶，其具有seqidno.40。22.如实施方案2所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.3。23.如实施方案22所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括a)seqidno.4或seqidno.161、b)seqidno.5以及c)seqidno.6或seqidno.162。24.如实施方案22所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.41。25.如实施方案22所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.42或43具有至少50％同一性。26.如实施方案22所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列与seqidno.42或43具有至少80％同一性。27.如实施方案22所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列是seq.idno.42或43。28.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括seqid.no.50。29.如实施方案28所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.51。30.如实施方案28所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.52。31.如实施方案28所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.53。32.如实施方案23-31中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.46。33.如实施方案28所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.54。34.如实施方案28-33中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.48。35.如实施方案28所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.55。36.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括seqid.no.56。37.如实施方案36所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.57。38.如实施方案36或37所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.51。39.如实施方案36或37所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.52。40.如实施方案36或37所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.53。41.如实施方案36所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.58。42.如实施方案36-41中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.48。43.如实施方案36所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.59。44.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括seqid.no.60。45.如实施方案44所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.61。46.如实施方案44所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.62。47.如实施方案44所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.63。48.如实施方案44-46中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸包括seqidno.46。49.如实施方案44-48中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.48。50.如实施方案44所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.64。51.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括seqid.no.65。52.如实施方案51所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.51。53.如实施方案51或52中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.66。54.如实施方案51-53中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.48。55.如实施方案51-54中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括以下中的至少一者：a)seqid.no.4或seqidno.161以及b)seqidno.5。56.如实施方案51-55中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.53。57.如实施方案51所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.67。58.如实施方案51所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.68。59.一种3-酮酰基-辅酶a合酶，其包含编码具有以下氨基酸序列的3-酮酰基-辅酶a合酶的异源核酸序列，所述氨基酸序列的特征在于包括seqid.no.69。60.如实施方案59所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列还包括seqidno.51。61.如实施方案59或60所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.52。62.如实施方案59或69所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.53。63.如实施方案56-62中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括以下中的至少一者：a)seqidno.5和b)seqidno.6或seqidno.162。64.如实施方案59-63中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.46。65.如实施方案59或60所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.70。66.如实施方案59-65中任一项所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括seqidno.48。67.如实施方案59所述的3-酮酰基-辅酶a合酶，其中所述氨基酸序列包括idno.71。68.一种酮酰基-辅酶a合酶，其具有seqidno.110或与seqidno.110具有至少80％同一性，所述酮酰基-辅酶a合酶在每种情况下包含特征i)-xiii)中的至少一种：i)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基30对齐的氨基酸残基是丙氨酸；ii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基39对齐的氨基酸残基是缬氨酸；iii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基69对齐的氨基酸残基是缬氨酸；iv)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基111对齐的氨基酸残基是半胱氨酸；v)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基152对齐的氨基酸残基是半胱氨酸、亮氨酸、甲硫氨酸或苏氨酸；vi)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基178对齐的氨基酸残基是亮氨酸；vii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基184对齐的氨基酸残基是异亮氨酸、亮氨酸、甲硫氨酸或缬氨酸；viii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基268对齐的氨基酸残基是丙氨酸；ix)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基271对齐的氨基酸残基是异亮氨酸；x)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基278对齐的氨基酸残基是精氨酸；xi)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基296对齐的氨基酸残基是丙氨酸；xii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基317对齐的氨基酸残基是丙氨酸；xiii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基328对齐的氨基酸残基是甘氨酸。69.一种酮酰基-辅酶a合酶，其具有seqidno.119或与seqidno.119具有至少80％同一性，所述酮酰基-辅酶a合酶在每种情况下包含特征i)-xliii)中的至少一种：i)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基18对齐的氨基酸残基是丙氨酸；ii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基22对齐的氨基酸残基是甲硫氨酸；iii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基30对齐的氨基酸残基是丙氨酸；iv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基38对齐的氨基酸残基是缬氨酸；v)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基39对齐的氨基酸残基是缬氨酸；vi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基51对齐的氨基酸残基是丙氨酸、半胱氨酸、天冬氨酸、组氨酸、异亮氨酸、赖氨酸、亮氨酸、甲硫氨酸、天冬酰胺、脯氨酸、谷氨酰胺、精氨酸、丝氨酸、苏氨酸、缬氨酸、色氨酸或酪氨酸；vii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基54对齐的氨基酸残基是缬氨酸；viii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基69对齐的氨基酸残基是缬氨酸；ix)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基83对齐的氨基酸残基是天冬酰胺；x)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基94对齐的氨基酸残基是苏氨酸、亮氨酸、谷氨酸或丙氨酸；xi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基111对齐的氨基酸残基是半胱氨酸；xii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基116对齐的氨基酸残基是甘氨酸；xiii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基127对齐的氨基酸残基是苏氨酸；xiv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基130对齐的氨基酸残基是甘氨酸；xv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基152对齐的氨基酸残基是半胱氨酸、亮氨酸、甲硫氨酸或苏氨酸；xvi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基154对齐的氨基酸残基是甘氨酸；xvii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基178对齐的氨基酸残基是亮氨酸或苏氨酸；xviii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基190对齐的氨基酸残基是苯丙氨酸或酪氨酸；xix)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基210对齐的氨基酸残基是缬氨酸；xx)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基223对齐的氨基酸残基是组氨酸；xxi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基231对齐的氨基酸残基是异亮氨酸；xxii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基232对齐的氨基酸残基是缬氨酸；xxiii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基236对齐的氨基酸残基是亮氨酸或甲硫氨酸；xxiv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基241对齐的氨基酸残基是脯氨酸；xxv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基268对齐的氨基酸残基是丙氨酸；xxvi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基271对齐的氨基酸残基是异亮氨酸；xxvii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基274对齐的氨基酸残基是谷氨酸；xxviii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基278对齐的氨基酸残基是精氨酸、谷氨酸、天冬氨酸、谷氨酰胺；xxix)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基280对齐的氨基酸残基是异亮氨酸；xxx)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基282对齐的氨基酸残基是甘氨酸；xxxi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基296对齐的氨基酸残基是丙氨酸；xxxii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基302对齐的氨基酸残基是苏氨酸；xxxiii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基312对齐的氨基酸残基是天冬氨酸；xxxiv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基313对齐的氨基酸残基是谷氨酸或甲硫氨酸；xxxv)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基315对齐的氨基酸残基是赖氨酸；xxxvi)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基317对齐的氨基酸残基是丙氨酸；xxxvii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基322对齐的氨基酸残基是甘氨酸；xxxviii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基328对齐的氨基酸残基是甘氨酸；xxxix)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基329对齐的氨基酸残基是甘氨酸；xl)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基344对齐的氨基酸残基是谷氨酸、天冬氨酸或天冬酰胺；xli)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基356对齐的氨基酸残基是甘氨酸或丝氨酸；xlii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基368对齐的氨基酸残基是精氨酸；并且xliii)所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基370对齐的氨基酸残基是苏氨酸。70.如实施方案69所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基184对齐的所述氨基酸残基是异亮氨酸、亮氨酸、甲硫氨酸或缬氨酸。71.如实施方案69所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基184对齐的所述氨基酸残基是异亮氨酸。72.如实施方案69-71中任一项所述的酮酰基-辅酶a合酶，其中：所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基268对齐的所述氨基酸残基是丙氨酸；所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基296对齐的所述氨基酸残基是丙氨酸；并且所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基328对齐的所述氨基酸残基是甘氨酸。73.如实施方案69-72中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基317对齐的所述氨基酸残基是丙氨酸。74.如实施方案70-73中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基30对齐的所述氨基酸残基是丙氨酸。75.如实施方案70-74中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基368对齐的所述氨基酸残基是精氨酸。76.如实施方案69-75中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基51对齐的氨基酸残基是丙氨酸、半胱氨酸、天冬氨酸、组氨酸、异亮氨酸、赖氨酸、亮氨酸、甲硫氨酸、天冬酰胺、脯氨酸、谷氨酰胺、精氨酸、丝氨酸、苏氨酸、缬氨酸、色氨酸或酪氨酸。77.如实施方案69-76中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.119的氨基酸残基51对齐的所述氨基酸残基是丙氨酸。78.一种3-酮酰基-辅酶a合酶，其具有选自由seqidno.121-157中的任一个组成的组的氨基酸序列。79.一种酮酰基-辅酶a合酶，其具有seqidno.110或与seqidno.110具有至少80％同一性，所述酮酰基-辅酶a合酶在每种情况下包含特征i)-xiii)中的至少一种：i)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基30对齐的氨基酸残基是丙氨酸；ii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基39对齐的氨基酸残基是缬氨酸；iii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基69对齐的氨基酸残基是缬氨酸；iv)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基111对齐的氨基酸残基是半胱氨酸；v)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基152对齐的氨基酸残基是半胱氨酸、亮氨酸、甲硫氨酸或苏氨酸；vi)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基178对齐的氨基酸残基是亮氨酸；vii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基184对齐的氨基酸残基是异亮氨酸、亮氨酸、甲硫氨酸或缬氨酸；viii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基268对齐的氨基酸残基是丙氨酸；ix)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基271对齐的氨基酸残基是异亮氨酸；x)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基278对齐的氨基酸残基是精氨酸；xi)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基296对齐的氨基酸残基是丙氨酸；xii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基317对齐的氨基酸残基是丙氨酸；xiii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基328对齐的氨基酸残基是甘氨酸。80.如实施方案79所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基184对齐的所述氨基酸残基是异亮氨酸、亮氨酸、甲硫氨酸或缬氨酸。81.如实施方案79所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基184对齐的所述氨基酸残基是异亮氨酸。82.如实施方案79-81中任一项所述的酮酰基-辅酶a合酶，其中：所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基268对齐的所述氨基酸残基是丙氨酸；所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基296对齐的所述氨基酸残基是丙氨酸；并且所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基328对齐的所述氨基酸残基是甘氨酸。83.如实施方案79-82中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基317对齐的所述氨基酸残基是丙氨酸。84.如实施方案80-83中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基30对齐的所述氨基酸残基是丙氨酸。85.如实施方案80-84中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.120的氨基酸残基368对齐的所述氨基酸残基是精氨酸。86.一种酮酰基-辅酶a合酶，其具有seqidno.110或与seqidno.110具有至少80％同一性，所述酮酰基-辅酶a合酶在每种情况下包含特征i)-xiii)中的至少一种：i)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基30对齐的氨基酸残基是丙氨酸；ii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基39对齐的氨基酸残基是缬氨酸；iii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基69对齐的氨基酸残基是缬氨酸；iv)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基111对齐的氨基酸残基是半胱氨酸；v)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基152对齐的氨基酸残基是半胱氨酸、亮氨酸、甲硫氨酸或苏氨酸；vi)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基178对齐的氨基酸残基是亮氨酸；vii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基184对齐的氨基酸残基是异亮氨酸、亮氨酸、甲硫氨酸或缬氨酸；viii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基268对齐的氨基酸残基是丙氨酸；ix)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基271对齐的氨基酸残基是异亮氨酸；x)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基278对齐的氨基酸残基是精氨酸；xi)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基296对齐的氨基酸残基是丙氨酸；xii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基317对齐的氨基酸残基是丙氨酸；xiii)所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基328对齐的氨基酸残基是甘氨酸。87.如实施方案86所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基184对齐的所述氨基酸残基是异亮氨酸、亮氨酸、甲硫氨酸或缬氨酸。88.如实施方案86所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基184对齐的所述氨基酸残基是异亮氨酸。89.如实施方案86-88中任一项所述的酮酰基-辅酶a合酶，其中：所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基268对齐的所述氨基酸残基是丙氨酸；所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基296对齐的所述氨基酸残基是丙氨酸；并且所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基328对齐的所述氨基酸残基是甘氨酸。90.如实施方案86-89中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基317对齐的所述氨基酸残基是丙氨酸。91.如实施方案87-90中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基30对齐的所述氨基酸残基是丙氨酸。92.如实施方案87-91中任一项所述的酮酰基-辅酶a合酶，其中所述酮酰基-辅酶a合酶的与seqidno.110的氨基酸残基368对齐的所述氨基酸残基是精氨酸。93.一种具有以下氨基酸序列的3-酮酰基-辅酶a合酶，所述氨基酸序列的特征在于包括seqid.no.170。94.一种3-酮酰基-辅酶a合酶基因，其编码如实施方案1-93中任一项所述的3-酮酰基-辅酶a合酶。95.一种经遗传修饰的细胞，其包含编码如实施方案1-93中任一项所述的3-酮酰基-辅酶a合酶的异源核酸序列。96.一种经遗传修饰的细胞，其包含如实施方案94所述的3-酮酰基-辅酶a合酶基因。97.如实施方案95或96所述的经遗传修饰的细胞，其中所述3-酮酰基-辅酶a合酶是丙二酰基-辅酶a依赖性的。98.如实施方案95-97中任一项所述的经遗传修饰的细胞，其还包含编码丙二酰基-辅酶a依赖性3-酮己酰基-辅酶a合酶的异源核酸序列。99.如实施方案98所述的经遗传修饰的细胞，其中所述编码丙二酰基-辅酶a依赖性3-酮己酰基-辅酶a合酶的异源核酸序列包含seqid.no.82。100.如实施方案98所述的经遗传修饰的细胞，其中所述编码丙二酰基-辅酶a依赖性3-酮己酰基-辅酶a合酶的异源核酸序列包含seqid.no.82，其中氨基酸残基100是亮氨酸，氨基酸残基147是丝氨酸、苏氨酸或苯丙氨酸，氨基酸残基217是缬氨酸，并且氨基酸残基323是缬氨酸。101.如实施方案95-100中任一项所述的经遗传修饰的细胞，其还包含编码丙二酰基-辅酶a依赖性3-酮丁酰基-辅酶a合酶的异源核酸序列。102.如实施方案101所述的经遗传修饰的细胞，其中所述编码丙二酰基-辅酶a依赖性3-酮丁酰基-辅酶a合酶的异源核酸序列包含seqid.no.83。103.如实施方案95-102中任一项所述的经遗传修饰的细胞，其还包含编码3-酮酰基-辅酶a还原酶的异源核酸序列。104.如实施方案103所述的经遗传修饰的细胞，其中所述3-酮酰基-辅酶a还原酶具有seq.id.no.103、seq.id.no.102或seq.id.no.101中的任一个或与seqidno:103、seqidno:102或seqidno:101中的任一个具有至少80％同一性。105.如实施方案95-104中任一项所述的经遗传修饰的细胞，其还包含编码3-羟基酰基-辅酶a脱水酶的异源核酸序列。106.如实施方案95-105中任一项所述的经遗传修饰的细胞，其还包含编码酶的异源核酸序列，所述酶使3-酮酰基-辅酶a还原以形成相应的3-羟基酰基-辅酶a并且使所述3-酮酰基-辅酶a脱水以形成相应的2-反式-烯酰基-辅酶a。107.如实施方案106所述的经遗传修饰的细胞，其中所述编码酶的异源核酸序列具有seq.id.no.98或与seq.id.no.98具有至少80％同一性，所述酶使3-酮酰基-辅酶a还原以形成相应的3-羟基酰基-辅酶a并且使所述3-羟基酰基-辅酶a脱水以形成相应的2-反式-烯酰基-辅酶a。108.如实施方案95-107中任一项所述的经遗传修饰的细胞，其还包含编码烯酰基-辅酶a还原酶的异源核酸序列。109.如实施方案95-108中任一项所述的经遗传修饰的细胞，其还包含编码酯合酶的异源核酸序列。110.如实施方案95-109中任一项所述的经遗传修饰的细胞，其还包含天然ldh基因的缺失。111.如实施方案95-110中任一项所述的经遗传修饰的细胞，其还包含天然丙酮酸甲酸裂解酶基因的缺失。112.如实施方案95-111中任一项所述的经遗传修饰的细胞，其还包含天然甲基乙二醛合酶基因的缺失。113.如实施方案95-112中任一项所述的经遗传修饰的细胞，其还包含天然磷酸转乙酰酶基因的缺失。114.如实施方案95-113中任一项所述的经遗传修饰的细胞，其还包含天然硫酯酶基因的缺失。115.如实施方案95-114中任一项所述的经遗传修饰的细胞，其还包含天然adhe基因的缺失。116.如实施方案95-115中任一项所述的经遗传修饰的细胞，其还包含天然atodaeb操纵子的缺失。117.如实施方案95-116中任一项所述的经遗传修饰的细胞，其还包含天然fadd基因的缺失。118.如实施方案95-111中任一项所述的经遗传修饰的细胞，其还包含天然磷酸转乙酰酶基因的缺失。119.如实施方案95-118中任一项所述的经遗传修饰的细胞，其还包含编码以下中的任一者的异源核酸序列：脂肪酰基-辅酶a还原酶、脂肪醛还原酶、酰基-acp还原酶、酰基-辅酶a:acp酰基转移酶、硫酯酶、酰基-辅酶a水解酶、羧酸还原酶、辅酶a水解酶、醛脱氢酶、羧酸还原酶和酰基-acp还原酶。120.如实施方案95-119中任一项所述的经遗传修饰的细胞，其是细菌。121.如实施方案120所述的经遗传修饰的细胞，其中所述细菌是大肠杆菌。122.一种用于制备一种或多种具有直链烷基的化合物的方法，所述方法包括在发酵培养基中培养如实施方案95-121中任一项所述的经遗传修饰的细胞以及从所述发酵培养基中回收所述具有直链烷基的化合物。123.如实施方案122所述的方法，其中至少40重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有6-10个碳原子。124.如实施方案122所述的方法，其中至少60重量％的所一种或多种述具有直链烷基的化合物在所述直链烷基中具有6-10个碳原子。125.如实施方案122-124中任一项所述的方法，其中所述一种或多种具有直链烷基的化合物是脂肪醇。126.如实施方案122-124中任一项所述的方法，其中所述一种或多种具有直链烷基的化合物是脂肪酰胺。127.如实施方案122-124中任一项所述的方法，其中所述一种或多种具有直链烷基的化合物是脂肪二酸或脂肪二酸酯。128.如实施方案122-124中任一项所述的方法，其中所述一种或多种具有直链烷基的化合物是脂肪酸。129.如实施方案122-124中任一项所述的方法，其中所述一种或多种具有直链烷基的化合物是脂肪酸酯。130.如实施方案129所述的方法，其中所述脂肪酸酯是甲酯和/或乙酯。131.如实施方案130所述的方法，其中所述发酵培养基包括甲醇和/或乙醇。132.如实施方案122-131中任一项所述的方法，其中至少60重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有8个碳原子。133.如实施方案122-131中任一项所述的方法，其中至少80重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有8个碳原子。134.如实施方案122-131中任一项所述的方法，其中至少90重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有8个碳原子。135.如实施方案122-131中任一项所述的方法，其中至少60重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有10个碳原子。136.如实施方案122-131中任一项所述的方法，其中至少80重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有10个碳原子。137.如实施方案122-131中任一项所述的方法，其中至少90重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有8个碳原子。138.如实施方案122-131中任一项所述的方法，其中至少95重量％的所述一种或多种具有直链烷基的化合物在所述直链烷基中具有8个碳原子。139.如实施方案122-138中任一项所述的方法，其中每小时每升发酵液产生至少0.05克的所述一种或多种具有直链烷基的化合物。140.如实施方案122-138中任一项所述的方法，其中每小时每升发酵液产生至少0.1克的所述一种或多种具有直链烷基的化合物。141.如实施方案122-138中任一项所述的方法，其中每小时每升发酵液产生至少0.25克的所述一种或多种具有直链烷基的化合物。142.如实施方案95-121中任一项所述的经遗传修饰的细胞用于产生一种或多种具有直链烷基的化合物的用途，其中至少60重量％的所述一种或多种化合物在所述直链烷基中具有6至10个碳原子。145.如实施方案95-121中任一项所述的经遗传修饰的细胞用于产生一种或多种具有直链烷基的化合物的用途，其中至少80重量％的所述一种或多种化合物在所述直链烷基中具有8至10个碳原子。146.一种细胞，其产生如实施方案1-93中任一项所述的3-酮酰基-辅酶a合酶。序列表<110>嘉吉公司(cargillincorporated)<120>产生c6-c10脂肪酸衍生物的经遗传修饰的细胞<130>n00513<150>us62/453,817<151>2017-02-02<160>204<170>patentinversion3.5<210>1<211>9<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(2)..(2)<223>leu或met<220><221>misc_feature<222>(8)..(8)<223>leu、val、ile或met<400>1leuxaavalasnvalgluilexaaser15<210>2<211>10<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(6)..(6)<223>ile或met<400>2valasnprogluilexaathrglyhismet1510<210>3<211>10<212>prt<213>人工(artificial)<220><223>3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(6)..(6)<223>ile、met、leu、thr、cys、val、gly、phe、asp、asn或tyr<400>3valasnprogluphexaaserproglnval1510<210>4<211>7<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his或asn<400>4aspmetxaavalalacysser15<210>5<211>7<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(7)..(7)<223>ala、ile、leu或val<400>5trpleuhisglnalaasnxaa15<210>6<211>7<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(4)..(4)<223>gly或ser<400>6alaasnthrxaaseralagly15<210>7<211>9<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(2)..(2)<223>leu或met<400>7leuxaavalasnvalgluileileser15<210>8<211>370<212>prt<213>申氏不动杆菌(acinetobacterschindleri)cip107287<400>8metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluilethrserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thrlysglnleuglulysasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnvalasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370<210>9<211>370<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基合酶<400>9metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluilevalserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnph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yglutyrglyvalleucysser340345350pheglyalaglytyrservalglyserileleuvalglnlysargval355360365ala<210>35<211>370<212>prt<213>约氏不动杆菌(acinetobacterjohnsonii)<400>35metglyileargilethrglythrglyleutyrhisprogluaspile151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleuaspasnalaasplysilealaserglygluleugluala354045argargglyserseralaglupheileglulysalaserglyvallys505560argargtyrvalvalglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalilealaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnleugln115120125argalatyrproalavalalailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalatyraspalailelysalaglyglyargargval165170175leuleuvalasnvalgluilethrserglyhisthrasptyrargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthraspserlysthrglyphegluilegluaspileasnleu210215220phethrglnpheserasnasnileargasnasnpheglypheleuasn225230235240leusergluvalaspalaaspilegluasnasnargphealaglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thralaglnleuglulysasnasnilealaprothraspvallysarg275280285phetrpleuhisglnalaasnalasermetasngluleuileleulys290295300leuvalvalglylysgluasnalaglnproaspleuvalproileile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaaspglnvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyalavalleuvallyslyshis355360365valala370<210>36<211>370<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<400>36metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalavallysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thrlysglnleuglulysasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnvalasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyglyalaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370<210>37<211>370<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<400>37metglyileargilethrglythrglyleutyrhisprogluaspile151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleuaspasnalaasplysilealaserglygluleugluala354045argargglyserseralaglupheileglulysalaserglyvallys505560argargtyrvalvalglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalilealaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnleugln115120125argalatyrproalavalalailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalatyraspalailelysalaglyglyargargval165170175leuleuvalasnvalgluilemetserglyhisthrasptyrargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthraspserlysthrglyphegluilegluaspileasnleu210215220phethrglnpheserasnasnileargasnasnpheglypheleuasn225230235240leusergluvalaspalaaspilegluasnasnargphealaglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thralaglnleuglulysasnasnilealaprothraspvallysarg275280285phetrpleuhisglnalaasnalasermetasngluleuileleulys290295300leuvalvalglylysgluasnalaglnproaspleuvalproileile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaaspglnvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyalavalleuvallyslyshis355360365valala370<210>38<211>370<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<400>38metglyileargilethrglythrglyleutyrhisprogluaspile151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleuaspasnalaasplysilealaserglygluleugluala354045argargglyserseralaglupheileglulysalaserglyvallys505560argargtyrvalvalglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalilealaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnleugln115120125argalatyrproalavalalailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalatyraspalailelysalaglyglyargargval165170175leuleuvalasnvalgluileileserglyhisthrasptyrargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthraspserlysthrglyphegluilegluaspileasnleu210215220phethrglnpheserasnasnileargasnasnpheglypheleuasn225230235240leusergluvalaspalaaspilegluasnasnargphealaglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thralaglnleuglulysasnasnilealaprothraspvallysarg275280285phetrpleuhisglnalaasnalasermetasngluleuileleulys290295300leuvalvalglylysgluasnalaglnproaspleuvalproileile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaaspglnvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyalavalleuvallyslyshis355360365valala370<210>39<211>370<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<400>39metglyileargilethrglythrglyleutyrhisprogluaspile151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleuaspasnalaasplysilealaserglygluleugluala354045argargglyserseralaglupheileglulysalaserglyvallys505560argargtyrvalvalglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalilealaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnleugln115120125argalatyrproalavalalailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalatyraspalailelysalaglyglyargargval165170175leuleuvalasnvalgluileleuserglyhisthrasptyrargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthraspserlysthrglyphegluilegluaspileasnleu210215220phethrglnpheserasnasnileargasnasnpheglypheleuasn225230235240leusergluvalaspalaaspilegluasnasnargphealaglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thralaglnleuglulysasnasnilealaprothraspvallysarg275280285phetrpleuhisglnalaasnalasermetasngluleuileleulys290295300leuvalvalglylysgluasnalaglnproaspleuvalproileile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaaspglnvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyalavalleuvallyslyshis355360365valala370<210>40<211>373<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(186)..(186)<223>ile或met<400>40mettyrasnvalvalileserglythrglyleutyrthrproalaser151015serileserasnaspgluleuvalgluserpheasnthrtyrvalhis202530argpheasnsergluasnalaalaalaileglualaglygluvalgln354045proleualagluserserseralapheileglulysalaserglyile505560lysserargtyrvalthrasplysalaglyileleuaspprogluarg65707580metvalproargileprogluargserasnaspglutrpserileleu859095cysglumetservallysalaalagluglualaleualaargalagly100105110lysthralaalaaspileaspglyvalilevalalacysserasnleu115120125glnargalatyrproalailealailegluvalglnalaalaleugly130135140ilelysglypheglypheaspmetasnvalalacysserseralathr145150155160pheglyileglnasnalavalasnserilelysleuglyglnalaarg165170175alaileleumetvalasnprogluilexaathrglyhismetasnphe180185190argaspargaspserhispheilepheglyaspalacysthralaval195200205valilegluarggluaspleualathrseralahisglntrpgluval210215220leuserthrlysleuvalthrglupheserasnasnileargasnasn225230235240pheglypheleuasnargthralagluglutyrmetserasnproasp245250255lysleupheileglngluglyarglysvalphelysgluvalcyspro260265270metvalalagluleuileglygluhisleusergluasnglyileala275280285valgluservallysargphetrpleuhisglnalaasnleuasnmet290295300asnhisleuilevalarglysleuleuglyargaspalathrgluglu305310315320glualaprovalileleuaspthrtyralaasnthrserseralagly325330335servalilealaphehislyshisglnaspaspleuproserglyser340345350leuglyvalleuserserpheglyalaglytyrserileglyserval355360365ileleuarglysarg370<210>41<211>10<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶的子序列<400>41valasnproglupheileserproglnval1510<210>42<211>372<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(186)..(186)<223>ile、met、leu、cys、val、gln、phe、asp、asn、try<400>42metglnglnvalvalileserglyserglyleuphethrproglnhis151015argileserasnglugluleuvalglnsertyrasnglntyrvalasp202530glnpheasnglugluhisalaalaalailealaalaglygluileglu354045alaleuglutyrserserthrglupheileglulysalaserglyile505560lysalaarghisvalleutyrlysaspglyileleuaspprolysile65707580methisprovalphearglysargglygluaspgluleuproglumet859095valglumetalavalglnalaalathrglnalaleualaglnalaasn100105110lysthralaalaaspileaspleuileilecysalaalaserasnmet115120125glnargprotyrproalaleuservalgluleuglnglnalaleugly130135140alaglyglytyralapheaspmetasnvalalacysserseralathr145150155160phealaileserasnalavalasnalaileargglyglythralalys165170175valvalleuvalvalasnprogluphexaaserproglnvalasptyr180185190argserargaspserhispheilepheglyaspvalcysthralathr195200205ileileglualaglusersercysserserglnglnalapheargile210215220leuglymetargleulysthrthrpheserasnasnileargcysasp225230235240ileglytyrthrgluhiscysphethrgluglnaspprolysalapro245250255phephelysglnglnglyarglysvalphelysgluleuleuproile260265270valalaaspvalileglnaspglumetalaalaglnasnleualapro275280285aspaspleulysargleutrpleuhisglnalaasnileasnmetasn290295300ilephealaalalyslysileleuglyargaspproleuprogluglu305310315320alaproleuvalleuaspthrtyralaasnthralaseralaglyser325330335ileilealaphehislystyrglnglnglyleuvalserglyasplys340345350alaileleucysserpheglyalaglytyrservalglycysvalval355360365leuglulyscys370<210>43<211>372<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶<400>43metglnglnvalvalileserglyserglyleuphethrproglnhis151015argileserasnglugluleuvalglnsertyrasnglntyrvalasp202530glnpheasnglugluhisalaalaalailealaalaglygluileglu354045alaleuglutyrserserthrglupheileglulysalaserglyile505560lysalaarghisvalleutyrlysaspglyileleuaspprolysile65707580methisprovalphearglysargglygluaspgluleuproglumet859095valglumetalavalglnalaalathrglnalaleualaglnalaasn100105110lysthralaalaaspileaspleuileilecysalaalaserasnmet115120125glnargprotyrproalaleuservalgluleuglnglnalaleugly130135140alaglyglytyralapheaspmetasnvalalacysserseralathr145150155160phealaileserasnalavalasnalaileargglyglythralalys165170175valvalleuvalvalasnproglupheileserproglnvalasptyr180185190argserargaspserhispheilepheglyaspvalcysthralathr195200205ileileglualaglusersercysserserglnglnalapheargile210215220leuglymetargleulysthrthrpheserasnasnileargcysasp225230235240ileglytyrthrgluhiscysphethrgluglnaspprolysalapro245250255phephelysglnglnglyarglysvalphelysgluleuleuproile260265270valalaaspvalileglnaspglumetalaalaglnasnleualapro275280285aspaspleulysargleutrpleuhisglnalaasnileasnmetasn290295300ilephealaalalyslysileleuglyargaspproleuprogluglu305310315320alaproleuvalleuaspthrtyralaasnthralaseralaglyser325330335ileilealaphehislystyrglnglnglyleuvalserglyasplys340345350alaileleucysserpheglyalaglytyrservalglycysvalval355360365leuglulyscys370<210>44<211>36<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(13)..(13)<223>leu、ile<220><221>misc_feature<222>(17)..(17)<223>ala或tyr<220><221>misc_feature<222>(22)..(22)<223>ala或ser<220><221>misc_feature<222>(24)..(24)<223>ala或gly<220><221>misc_feature<222>(29)..(29)<223>leu或met<220><221>misc_feature<222>(35)..(35)<223>leu、val、ile或met<400>44aspmetxaavalalacysseralaalathrpheglyxaalysglnala151015xaaaspalailelysxaaglyxaaargargvalleuxaavalasnval202530gluilexaaser35<210>45<211>89<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(13)..(13)<223>leu或ile<220><221>misc_feature<222>(17)..(17)<223>ala或tyr<220><221>misc_feature<222>(22)..(22)<223>ala或ser<220><221>misc_feature<222>(24)..(24)<223>ala或gly<220><221>misc_feature<222>(29)..(29)<223>leu或met<220><221>misc_feature<222>(35)..(35)<223>leu、val、ile或met<220><221>misc_feature<222>(37)..(37)<223>ala或gly<220><221>misc_feature<222>(41)..(41)<223>phe或tyr<220><221>misc_feature<222>(45)..(45)<223>asp或gly<220><221>misc_feature<222>(61)..(61)<223>glu或asn<220><221>misc_feature<222>(63)..(63)<223>asp或glu<220><221>misc_feature<222>(64)..(64)<223>ser或thr<220><221>misc_feature<222>(71)..(71)<223>leu或glu<220><221>misc_feature<222>(73)..(73)<223>ser或ile<220><221>misc_feature<222>(74)..(74)<223>glu或asn<220><221>misc_feature<222>(89)..(89)<223>phe或tyr<400>45aspmetxaavalalacysseralaalathrpheglyxaalysglnala151015xaaaspalailelysxaaglyxaaargargvalleuxaavalasnval202530gluilexaaserxaahisthraspxaaargserargxaacyshisphe354045ilepheglyaspvalalathralaserileilegluxaathrxaaxaa505560lysthrglyphegluilexaaaspxaaxaaleuphethrglnpheser65707580asnasnileargasnasnpheglyxaa85<210>46<211>49<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(7)..(7)<223>ala、val、ile或leu<220><221>misc_feature<222>(8)..(8)<223>ser或asn<220><221>misc_feature<222>(15)..(15)<223>lys、met或gly<220><221>misc_feature<222>(17)..(17)<223>ile或val<220><221>misc_feature<222>(20)..(20)<223>glu或lys<220><221>misc_feature<222>(22)..(22)<223>his或asn<220><221>misc_feature<222>(24)..(24)<223>gln或lys<220><221>misc_feature<222>(25)..(25)<223>pro或ser<220><221>misc_feature<222>(26)..(26)<223>gly、asp或glu<220><221>misc_feature<222>(28)..(28)<223>val或ala<220><221>misc_feature<222>(30)..(30)<223>met、ile或leu<220><221>misc_feature<222>(31)..(31)<223>ile或val<220><221>misc_feature<222>(33)..(33)<223>asp或asn<220><221>misc_feature<222>(39)..(39)<223>ser或gly<220><221>misc_feature<222>(45)..(45)<223>ile或val<400>46trpleuhisglnalaasnxaaxaametasngluleuileleuxaaleu151015xaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaaleu202530xaagluphealaasnthrxaaseralaglyvalilexaaalaleuhis354045arg<210>47<211>189<212>prt<213>人工(artificial)<220><223>3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(13)..(13)<223>ile或leu<220><221>misc_feature<222>(17)..(17)<223>ala或tyr<220><221>misc_feature<222>(22)..(22)<223>ala或ser<220><221>misc_feature<222>(24)..(24)<223>gly或ala<220><221>misc_feature<222>(29)..(29)<223>leu或met<220><221>misc_feature<222>(35)..(35)<223>leu、val、ile或met<220><221>misc_feature<222>(37)..(37)<223>ala或gly<220><221>misc_feature<222>(41)..(41)<223>phe或tyr<220><221>misc_feature<222>(45)..(45)<223>asp或gly<220><221>misc_feature<222>(61)..(61)<223>glu或asn<220><221>misc_feature<222>(63)..(63)<223>asp或glu<220><221>misc_feature<222>(64)..(64)<223>ser或thr<220><221>misc_feature<222>(71)..(71)<223>leu或glu<220><221>misc_feature<222>(73)..(73)<223>ser或ile<220><221>misc_feature<222>(74)..(74)<223>glu或asn<220><221>misc_feature<222>(89)..(89)<223>phe或tyr<220><221>misc_feature<222>(92)..(92)<223>thr或leu<220><221>misc_feature<222>(95)..(95)<223>val或缺失<220><221>misc_feature<222>(96)..(96)<223>asn或asp<220><221>misc_feature<222>(100)..(100)<223>asp或glu<220><221>misc_feature<222>(101)..(101)<223>asp或asn<220><221>misc_feature<222>(102)..(102)<223>lys或asn<220><221>misc_feature<222>(105)..(105)<223>ala或arg<220><221>misc_feature<222>(119)..(119)<223>ala或val<220><221>misc_feature<222>(122)..(122)<223>ile或met<220><221>misc_feature<222>(125)..(125)<223>ala或lys<220><221>misc_feature<222>(129)..(129)<223>lyr或arg<220><221>misc_feature<222>(131)..(131)<223>asn或gln<220><221>misc_feature<222>(133)..(133)<223>ala或glu<220><221>misc_feature<222>(136)..(136)<223>gly、asp或asn<220><221>misc_feature<222>(147)..(147)<223>ala、val、ile或leu<220><221>misc_feature<222>(148)..(148)<223>ser或asn<220><221>misc_feature<222>(155)..(155)<223>lys、met或gln<220><221>misc_feature<222>(157)..(157)<223>ile或val<220><221>misc_feature<222>(160)..(160)<223>glu或lys<220><221>misc_feature<222>(162)..(162)<223>asn或his<220><221>misc_feature<222>(164)..(164)<223>lys或gln<220><221>misc_feature<222>(165)..(165)<223>pro或ser<220><221>misc_feature<222>(166)..(166)<223>gly、asp或glu<220><221>misc_feature<222>(168)..(168)<223>val或ala<220><221>misc_feature<222>(170)..(170)<223>met、ile或leu<220><221>misc_feature<222>(171)..(171)<223>ile或val<220><221>misc_feature<222>(173)..(173)<223>asn或asp<220><221>misc_feature<222>(179)..(179)<223>ser或gly<220><221>misc_feature<222>(185)..(185)<223>ile或val<400>47aspmetxaavalalacysseralaalathrpheglyxaalysglnala151015xaaaspalailelysxaaglyxaaargargvalleuxaavalasnval202530gluilexaaserxaahisthraspxaaargserargxaacyshisphe354045ilepheglyaspvalalathralaserileilegluxaathrxaaxaa505560lysthrglyphegluilexaaaspxaaxaaleuphethrglnpheser65707580asnasnileargasnasnpheglyxaaleuasnxaasergluxaaxaa859095alaaspilexaaxaaxaaargphexaaglnaspglyarglysvalphe100105110lysgluvalcysproleuxaaalalysxaailethrxaaglnleuglu115120125xaaasnxaailexaaprothrxaavallysargphetrpleuhisgln130135140alaasnxaaxaametasngluleuileleuxaaleuxaavalglyxaa145150155160gluxaaalaxaaxaaxaaleuxaaproxaaxaaleuxaaglupheala165170175asnthrxaaseralaglyvalilexaaalaleuhisarg180185<210>48<211>149<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>als或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<400>48metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyr145<210>49<211>338<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>valine或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asn或asp<220><221>misc_feature<222>(250)..(250)<223>asp或glu<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<400>49metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisarg<210>50<211>7<212>prt<213>人工(artificial)<220><223>突变型3-酮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<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(382)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<400>71metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysileile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisarg<210>72<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>72metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluilethrserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnvalasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370<210>73<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>73metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluilethrserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thrlysglnleuglulysasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370<21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00105110promettrpglucyshisleuilegluglyileargglyargargphe115120125alavaltyrtyrlysalahishiscysmetleuaspglyvalalaala130135140metargmetcysvallyssertyrserpheaspprothralathrglu145150155160metproproiletrpalaileserlysaspvalthrproalaargglu165170175thrglnalaproalaalaglyaspleuvalhisserleuserglnleu180185190valgluglyalaglyargglnleualathrvalprothrleuilearg195200205gluleuglylysasnleuleulysalaargaspaspseraspalagly210215220leuilepheargalaproproserileleuasnglnargilethrgly225230235240serargargphealaalaglnsertyralaleugluargphelysala245250255ileglylysalapheglnalathrvalasnaspvalvalleualaval260265270cysglyseralaleuargasntyrleuleuserargglnalaleupro275280285aspglnproleuilealametalaprometserileargglnaspasp290295300seraspserglyasnglnilealametileleualaasnleuglythr305310315320hisilealaaspprovalargargleugluleuthrglnalaserala325330335arggluserlysgluargpheargglnmetthrprogluglualaval340345350asntyrthralaleuthrleualaproserglyleuasnleuleuthr355360365glyleualaprolystrpglnalapheasnvalvalileserasnval370375380proglyproasnlysproleutyrtrpasnglyalaargleuglugly385390395400mettyrprovalserileprovalasptyralaalaleuasnilethr405410415leuvalsertyrargaspglnleuglupheglyphethralacysarg420425430argthrleuprosermetglnargleuleuasptyrilegluglngly435440445ilealagluleuglulysalaalaglyval450455<210>105<211>10<212>prt<213>人工(artificial)<220><223>各种天然存在的3-酮酰基-辅酶a合酶的子序列<400>105valasnprogluilecysthrglyhismet1510<210>106<211>10<212>prt<213>人工(artificial)<220><223>各种天然存在的3-酮酰基-辅酶a合酶的子序列<400>106valasnprogluphealaserproglnval1510<210>107<211>9<212>prt<213>人工(artificial)<220><223>各种天然存在的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>val或ala<220><221>misc_feature<222>(9)..(9)<223>ala或lys<400>107proleuxaaalalysmetilethrxaa15<210>108<211>7<212>prt<213>人工(artificial)<220><223>各种天然存在的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(6)..(6)<223>gln或asn<400>108glnleuglulysasnxaaile15<210>109<211>10<212>prt<213>人工(artificial)<220><223>来自各种天然存在的3-酮酰基-辅酶a合酶的子序列<400>109leuhisglnalaasnvalasnmetasnglu1510<210>110<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或gln<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(223)<223>ser或ile<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>110metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>111<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、asp或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>thr、leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或glu<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>111metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>112<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>thr、leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>tyr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>112metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>113<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(109)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、arg或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>113metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuleuvalasnvalgluilethrserglyhisthrasppheargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>114<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>thr、leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(319)..(391)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gln<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>114metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleualaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>115<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>thr、leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile、leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或asn<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>115metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysileile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>116<211>14<212>prt<213>申氏不动杆菌(acinetobacterschindleri)cip107287<400>116leumetvalasnvalgluilethrserglyhisthraspphe1510<210>117<211>215<212>dna<213>大肠杆菌(escherichiacoli)<400>117gatcttgatatcaaacgaacgttttagcaggactgtcgtcggttgccaaccatctgcgag60caaagcatggcgttttgttgcgcgggatcagcaagcctagcggcagttgtttacgctttt120attacagatttaataaattaccacattttaagaatattattaatctgtaatatatcttta180acaatctcaggttaaaaactttcctgttttcaacg215<210>118<211>121<212>dna<213>大肠杆菌(escherichiacoli)<400>118aaatcagactgaagactttatctctctgtcataaaactgtcatattccttacatataact60gtcacctgtttgtcctattttgcttctcgtagccaacaaacaatgctttatgaatcctcc120c121<210>119<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile,phe、thr或ser<220><221>misc_feature<222>(18)..(18)<223>thr或ala<220><221>misc_feature<222>(22)..(22)<223>leu或met<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu、gln、glx、arg或gly<220><221>misc_feature<222>(33)..(33)<223>phe或gln<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(38)..(38)<223>ala或val<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp、gly、ala、cys、his、ile、lys、leu、met、asn、pro、gln、arg、ser、thr、val、trp或tyr<220><221>misc_feature<222>(54)..(54)<223>ala或val<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>lys、gln、glu或gly<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn、leu、asn、his或phe<220><221>misc_feature<222>(85)..(85)<223>pro、his、arg、gln或asp<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile、leu,thr、glu或ala<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(116)..(116)<223>asp或gly<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile、leu或thr<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(154)..(154)<223>ala或gly<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu、met或thr<220><221>misc_feature<222>(184)..(184)<223>leu、val、ile、met、cys或thr<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu、asn或val<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu、his、gln、ser或asn<220><221>misc_feature<222>(227)..(227)<223>gln、lys、thr或asp<220><221>misc_feature<222>(231)..(231)<223>asn或ile<220><221>misc_feature<222>(232)..(232)<223>ile或val<220><221>misc_feature<222>(236)..(236)<223>phe或leu<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr、leu或pro<220><221>misc_feature<222>(243)..(243)<223>glu、phe、gly或pro<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala、lys或glu<220><221>misc_feature<222>(278)..(278)<223>lys、glu、asp、gln或arg<220><221>misc_feature<222>(280)..(280)<223>gln、ala、his、glu或asn<220><221>misc_feature<222>(282)..(282)<223>glu、ala或gly<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(302)..(302)<223>ile或thr<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(312)..(312)<223>ala或asp<220><221>misc_feature<222>(313)..(313)<223>lys、gln、glu或met<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp、glu或lys<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn、asp或gly<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(329)..(329)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu、pro、asp或asn<220><221>misc_feature<222>(356)..(356)<223>ala、gly或ser<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<220><221>misc_feature<222>(370)..(370)<223>ala或thr<400>119metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrxaapheserasnasnileargasnasnpheglyxaaleuasn225230235240xaaserxaaxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyxaaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valxaa370<210>120<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile或phe<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu或gln<220><221>misc_feature<222>(33)..(33)<223>phe或gln<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp或gly<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln或lys<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn或leu<220><221>misc_feature<222>(85)..(85)<223>pro或his<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile或leu<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile或leu<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu或met<220><221>misc_feature<222>(184)..(184)<223>thr、leu、val、ile或met<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu或asn<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu或asn<220><221>misc_feature<222>(238)..(238)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr或leu<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asp或glu<220><221>misc_feature<222>(250)..(250)<223>asp或asn<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala或lys<220><221>misc_feature<222>(278)..(278)<223>lys或arg<220><221>misc_feature<222>(280)..(280)<223>gln或asn<220><221>misc_feature<222>(282)..(282)<223>glu或ala<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(313)..(313)<223>lys或gln<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp或glu<220><221>misc_feature<222>(317)..(317)<223>val或ala<220><221>misc_feature<222>(319)..(319)<223>met、ile或leu<220><221>misc_feature<222>(320)..(320)<223>ile或val<220><221>misc_feature<222>(322)..(322)<223>asn或asp<220><221>misc_feature<222>(328)..(328)<223>ser或gly<220><221>misc_feature<222>(334)..(334)<223>ile或val<220><221>misc_feature<222>(341)..(341)<223>his、asp或asn<220><221>misc_feature<222>(342)..(342)<223>glu或gln<220><221>misc_feature<222>(344)..(344)<223>glu或asn<220><221>misc_feature<222>(362)..(362)<223>ser或ala<220><221>misc_feature<222>(363)..(363)<223>val或ile<220><221>misc_feature<222>(366)..(366)<223>gln或lys<220><221>misc_feature<222>(368)..(368)<223>arg或his<400>120metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilethrasnglugluleuvalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnalaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserseralaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluaspileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyraspmetxaavalalacysseralaalathrphe145150155160glyxaalysglnalaxaaaspalailelysxaaglyxaaargargval165170175leuxaavalasnvalgluilexaaserxaahisthraspxaaargser180185190argxaacyshispheilepheglyaspvalalathralaserileile195200205gluxaathrxaaxaalysthrglyphegluilexaaaspxaaxaaleu210215220phethrglnpheserasnasnileargasnasnpheglyxaaleuasn225230235240xaasergluxaaxaaalaaspilexaaxaaxaaargphexaaglnasp245250255glyarglysvalphelysgluvalcysproleuxaaalalysxaaile260265270thrxaaglnleugluxaaasnxaailexaaprothrxaavallysarg275280285phetrpleuhisglnalaasnxaaxaametasngluleuileleuxaa290295300leuxaavalglyxaagluxaaalaxaaxaaxaaleuxaaproxaaxaa305310315320leuxaagluphealaasnthrxaaseralaglyvalilexaaalaleu325330335hisargthralaxaaxaavalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyxaaxaaleuvalxaalysxaa355360365valala370<210>121<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>121metglyileargilethrglythrglyleuphehisprothraspphe151015ileth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aasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>150<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>150metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalglycysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuthrleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>151<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>151metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserservalglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalglycysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuthrleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>152<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>152metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserservalglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuthrleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>153<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>153metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserservalglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>154<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>154metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuthrleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>155<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>155metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserservalglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalglycysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>156<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>156metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalglycysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysileile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>157<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(51)..(51)<223>ala、cys、asp、his、ile、lys、leu、met、asn、pro、gln、arg、ser、thr、val、trp或tyr<400>157metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargxaaserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysmetile260265270thrlysglnleuglulysasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370<210>158<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(51)..(51)<223>cys、asp、glu、phe,his、ile、lys、met、asn、pro、arg、ser、thr、trp或tyr<400>158metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargxaaserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysmetile260265270thrlysglnleugluargasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>159<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<220><221>misc_feature<222>(16)..(16)<223>phe、thr、val或ser<220><221>misc_feature<222>(32)..(32)<223>gln、leu、arg或gly<220><221>misc_feature<222>(64)..(64)<223>lys、gln、glu或gly<220><221>misc_feature<222>(83)..(83)<223>leu、asn、his或phe<220><221>misc_feature<222>(85)..(85)<223>his、arg、gln或asp<220><221>misc_feature<222>(94)..(94)<223>ile、leu、glu或ala<220><221>misc_feature<222>(184)..(184)<223>thr、cys、leu、met或ile<220><221>misc_feature<222>(223)..(223)<223>glu、his、gln或ser<220><221>misc_feature<222>(227)..(227)<223>gln、lys、thr或asp<220><221>misc_feature<222>(243)..(243)<223>glu、phe、gly或pro<220><221>misc_feature<222>(278)..(278)<223>lys、glu、asp或gln<220><221>misc_feature<222>(280)..(280)<223>gln、ala、his或glu<220><221>misc_feature<222>(344)..(344)<223>asn、glu、pro或asp<400>159metglyileargilethrglythrglyleuphehisprothraspxaa151015ilethrasnglugluleuvalgluserleuasnalatyrvalgluxaa202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargalaserseralaglupheileglulysalaserglyilexaa505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproxaaleuxaagluargserasnaspgluleuserxaaglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluilexaaserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilexaaleu210215220phethrxaapheserasnasnileargasnasnpheglytyrleuasn225230235240leuserxaavalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleuvalalalysmetile260265270thrlysglnleugluxaaasnxaailegluprothrasnvallysarg275280285phetrpleuhisglnalaasnvalasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrserseralaglyvalileilealaleu325330335hisargthralaasngluvalxaaaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370<210>160<211>9<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(2)..(2)<223>leu、met或thr<220><221>misc_feature<222>(8)..(8)<223>leu、val、ile或met<400>160leuxaavalasnvalgluilexaaser15<210>161<211>7<212>prt<213>人工(artificial)<220><223>3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his或asn<220><221>misc_feature<222>(5)..(5)<223>ala或gly<400>161aspmetxaavalxaacysser15<210>162<211>7<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(4)..(4)<223>gly或ser<220><221>misc_feature<222>(5)..(5)<223>ser或gly<400>162alaasnthrxaaxaaalagly15<210>163<211>9<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(2)..(2)<223>leu、met或thr<400>163leuxaavalasnvalgluileileser15<210>164<211>36<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(5)..(5)<223>ala或gly<220><221>misc_feature<222>(13)..(13)<223>leu或ile<220><221>misc_feature<222>(17)..(17)<223>ala或tyr<220><221>misc_feature<222>(22)..(22)<223>ala或ser<220><221>misc_feature<222>(24)..(24)<223>ala或gly<220><221>misc_feature<222>(29)..(29)<223>leu、met或thr<220><221>misc_feature<222>(35)..(35)<223>lauval、ile、met或thr<400>164aspmetxaavalalacysseralaalathrpheglyxaalysglnala151015xaaaspalailelysxaaglyxaaargargvalleuxaavalasnval202530gluilexaaser35<210>165<211>89<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(5)..(5)<223>ala或gly<220><221>misc_feature<222>(13)..(13)<223>leu或ile<220><221>misc_feature<222>(17)..(17)<223>ala或tyr<220><221>misc_feature<222>(22)..(22)<223>ala或ser<220><221>misc_feature<222>(24)..(24)<223>ala或gly<220><221>misc_feature<222>(29)..(29)<223>leu、met或thr<220><221>misc_feature<222>(35)..(35)<223>leu、val、ile或met<220><221>misc_feature<222>(37)..(37)<223>ala或gly<220><221>misc_feature<222>(41)..(41)<223>phe或tyr<220><221>misc_feature<222>(45)..(45)<223>asp或gly<220><221>misc_feature<222>(61)..(61)<223>glu、asn或val<220><221>misc_feature<222>(63)..(63)<223>asp或glu<220><221>misc_feature<222>(64)..(64)<223>ser或thr<220><221>misc_feature<222>(71)..(71)<223>leu或glu<220><221>misc_feature<222>(73)..(73)<223>ser或ile<220><221>misc_feature<222>(74)..(74)<223>glu、asn、his、gln或ser<220><221>misc_feature<222>(78)..(78)<223>gln、lys、thr或asp<220><221>misc_feature<222>(82)..(82)<223>asn或ile<220><221>misc_feature<222>(83)..(83)<223>ile或val<220><221>misc_feature<222>(87)..(87)<223>phe或leu<220><221>misc_feature<222>(89)..(89)<223>phe或tyr<400>165aspmetxaavalxaacysseralaalathrpheglyxaalysglnala151015xaaaspalailelysxaaglyxaaargargvalleuxaavalasnval202530gluilexaaserxaahisthraspxaaargserargxaacyshisphe354045ilepheglyaspvalalathralaserileilegluxaathrxaaxaa505560lysthrglyphegluilexaaaspxaaxaaleuphethrxaapheser65707580asnxaaxaaargasnasnxaaglyxaa85<210>166<211>49<212>prt<213>人工(artificial)<220><223>突变的3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(7)..(7)<223>ala、val、ile或leu<220><221>misc_feature<222>(8)..(8)<223>ser或asn<220><221>misc_feature<222>(13)..(13)<223>ile或thr<220><221>misc_feature<222>(15)..(15)<223>lys、met或gly<220><221>misc_feature<222>(17)..(17)<223>ile或val<220><221>misc_feature<222>(20)..(20)<223>glu或lys<220><221>misc_feature<222>(22)..(22)<223>his或asn<220><221>misc_feature<222>(23)..(23)<223>ala或asp<220><221>misc_feature<222>(24)..(24)<223>gln、lys、glu或met<220><221>misc_feature<222>(25)..(25)<223>pro或ser<220><221>misc_feature<222>(26)..(26)<223>glu、gly、asp或lys<220><221>misc_feature<222>(28)..(28)<223>val或ala<220><221>misc_feature<222>(30)..(30)<223>met、ile或leu<220><221>misc_feature<222>(31)..(31)<223>ile或val<220><221>misc_feature<222>(33)..(33)<223>asp、asn或gly<220><221>misc_feature<222>(39)..(39)<223>ser或gly<220><221>misc_feature<222>(40)..(40)<223>ser或gly<220><221>misc_feature<222>(45)..(45)<223>ile或val<400>166trpleuhisglnalaasnxaaxaametasngluleuxaaleuxaaleu151015xaavalglyxaagluxaaxaaxaaxaaxaaleuxaaproxaaxaaleu202530xaagluphealaasnthrxaaxaaalaglyvalilexaaalaleuhis354045arg<210>167<211>189<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(3)..(3)<223>ile或leu<220><221>misc_feature<222>(5)..(5)<223>ala或gly<220><221>misc_feature<222>(13)..(13)<223>ile或leu<220><221>misc_feature<222>(17)..(17)<223>ala或tyr<220><221>misc_feature<222>(22)..(22)<223>ala或ser<220><221>misc_feature<222>(24)..(24)<223>gly或ala<220><221>misc_feature<222>(29)..(29)<223>leu、met或thr<220><221>misc_feature<222>(35)..(35)<223>leu、val、ile、met或cys<220><221>misc_feature<222>(37)..(37)<223>ala或gly<220><221>misc_feature<222>(41)..(41)<223>phe或tyr<220><221>misc_feature<222>(45)..(45)<223>asp或gly<220><221>misc_feature<222>(61)..(61)<223>glu、asn或val<220><221>misc_feature<222>(63)..(63)<223>asp或glu<220><221>misc_feature<222>(64)..(64)<223>ser或thr<220><221>misc_feature<222>(71)..(71)<223>leu或glu<220><221>misc_feature<222>(73)..(73)<223>ser或ile<220><221>misc_feature<222>(74)..(74)<223>glu或asn<220><221>misc_feature<222>(78)..(78)<223>gln、lys、thr或asp<220><221>misc_feature<222>(82)..(82)<223>asn或ile<220><221>misc_feature<222>(83)..(83)<223>ile或val<220><221>misc_feature<222>(87)..(87)<223>phe或leu<220><221>misc_feature<222>(89)..(89)<223>phe或tyr<220><221>misc_feature<222>(92)..(92)<223>thr、leu或pro<220><221>misc_feature<222>(94)..(94)<223>glu、phe、gly或pro<220><221>misc_feature<222>(95)..(95)<223>val或缺失<220><221>misc_feature<222>(96)..(96)<223>asn或asp<220><221>misc_feature<222>(100)..(100)<223>asp或glu<220><221>misc_feature<222>(101)..(101)<223>asp或asn<220><221>misc_feature<222>(102)..(102)<223>lys或asn<220><221>misc_feature<222>(105)..(105)<223>ala或arg<220><221>misc_feature<222>(119)..(119)<223>ala或val<220><221>misc_feature<222>(122)..(122)<223>ile或met<220><221>misc_feature<222>(125)..(125)<223>ala、lys或glu<220><221>misc_feature<222>(129)..(129)<223>lys、arg、glu、asp或gly<220><221>misc_feature<222>(131)..(131)<223>asn、gln、ala、his或glu<220><221>misc_feature<222>(133)..(133)<223>ala、glu或gly<220><221>misc_feature<222>(136)..(136)<223>gly、asp或asn<220><221>misc_feature<222>(147)..(147)<223>ala、val、ile或leu<220><221>misc_feature<222>(148)..(148)<223>ser或asn<220><221>misc_feature<222>(153)..(153)<223>ile或thr<220><221>misc_feature<222>(155)..(155)<223>lys、met或gln<220><221>misc_feature<222>(157)..(157)<223>ile或val<220><221>misc_feature<222>(160)..(160)<223>glu或lys<220><221>misc_feature<222>(162)..(162)<223>asn或his<220><221>misc_feature<222>(163)..(163)<223>ala或asp<220><221>misc_feature<222>(164)..(164)<223>lys、gln、glu、met<220><221>misc_feature<222>(165)..(165)<223>pro或ser<220><221>misc_feature<222>(166)..(166)<223>gly、asp、glu或lys<220><221>misc_feature<222>(168)..(168)<223>val或ala<220><221>misc_feature<222>(170)..(170)<223>met、ile或leu<220><221>misc_feature<222>(171)..(171)<223>ile或val<220><221>misc_feature<222>(173)..(173)<223>asn、asp或gly<220><221>misc_feature<222>(179)..(179)<223>ser或gly<220><221>misc_feature<222>(180)..(180)<223>ser或gly<220><221>misc_feature<222>(185)..(185)<223>ile或val<400>167aspmetxaavalxaacysseralaalathrpheglyxaalysglnala151015xaaaspalailelysxaaglyxaaargargvalleuxaavalasnval202530gluilexaaserxaahisthraspxaaargserargxaacyshisphe354045ilepheglyaspvalalathralaserileilegluxaathrxaaxaa505560lysthrglyphegluilexaaaspxaaxaaleuphethrglnpheser65707580asnxaaxaaargasnasnxaaglyxaaleuasnxaasergluxaaxaa859095alaaspilexaaxaaxaaargphexaaglnaspglyarglysvalphe100105110lysgluvalcysproleuxaaalalysxaailethrxaaglnleuglu115120125xaaasnxaailexaaprothrxaavallysargphetrpleuhisgln130135140alaasnxaaxaametasngluleuxaaleuxaaleuxaavalglyxaa145150155160gluxaaxaaxaaxaaxaaleuxaaproxaaxaaleuxaaglupheala165170175asnthrxaaxaaalaglyvalilexaaalaleuhisarg180185<210>168<211>149<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile、phe、thr、val或ser<220><221>misc_feature<222>(18)..(18)<223>thr或ala<220><221>misc_feature<222>(22)..(22)<223>leu或met<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu、gln、arg或gly<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(38)..(38)<223>ala或val<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp、gly、cys、asp、glu、phe、his、ile、lys、met、asn、pro、gln、arg、ser、thr、val、trp、tyr<220><221>misc_feature<222>(54)..(54)<223>val或ala<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln、lys、glu或gly<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn、leu、his或phe<220><221>misc_feature<222>(85)..(85)<223>pro、his、arg、gln或asp<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile、leu、thr、glu或ala<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(116)..(116)<223>asp或glu<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile、leu或thr<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<400>168metglyileargilethrglythrglyleuxaahisproxaaxaaxaa151015ilexaaasnglugluxaavalgluserleuasnalatyrxaagluxaa202530xaaasnxaaxaaasnxaaxaalysilealaxaaglygluxaagluxaa354045xaaargxaaserserxaaglupheileglulysalaserglyxaaxaa505560argargtyrvalxaaglulysxaaglyileleuaspprolysargleu65707580argproxaaleuxaagluargxaaxaaaspgluxaaserxaaglnala859095glutrpglyxaaxaaalaalalysglnalametxaaasnalaxaaval100105110thralagluxaaileaspxaavalileleusercysserasnxaagln115120125argxaatyrproalaxaaalailegluileglnthralaleuglyile130135140xaaglytyralatyr145<210>169<211>338<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶的子序列<220><221>misc_feature<222>(11)..(11)<223>phe或tyr<220><221>misc_feature<222>(14)..(14)<223>glu或thr<220><221>misc_feature<222>(15)..(15)<223>his、asn或asp<220><221>misc_feature<222>(16)..(16)<223>val、ile、phe、thr或ser<220><221>misc_feature<222>(18)..(18)<223>thr或ala<220><221>misc_feature<222>(22)..(22)<223>leu或met<220><221>misc_feature<222>(30)..(30)<223>val或ala<220><221>misc_feature<222>(32)..(32)<223>leu、gln、argandgly<220><221>misc_feature<222>(33)..(33)<223>phe或tyr<220><221>misc_feature<222>(35)..(35)<223>his或leu<220><221>misc_feature<222>(36)..(36)<223>glu或asp<220><221>misc_feature<222>(38)..(38)<223>ala或val<220><221>misc_feature<222>(39)..(39)<223>asp或val<220><221>misc_feature<222>(43)..(43)<223>ala或ser<220><221>misc_feature<222>(46)..(46)<223>ile或leu<220><221>misc_feature<222>(48)..(48)<223>glu或ala<220><221>misc_feature<222>(49)..(49)<223>arg或leu<220><221>misc_feature<222>(51)..(51)<223>asp、gly、ala、cys、glu、phe、his、ile、lys、leu、met、asn、pro、gln、arg、ser、thr、val、trp或try<220><221>misc_feature<222>(54)..(54)<223>val或ala<220><221>misc_feature<222>(63)..(63)<223>val或ile<220><221>misc_feature<222>(64)..(64)<223>gln、lys、glu或gly<220><221>misc_feature<222>(69)..(69)<223>ala或val<220><221>misc_feature<222>(72)..(72)<223>ser或thr<220><221>misc_feature<222>(83)..(83)<223>asn、leu、his或phe<220><221>misc_feature<222>(85)..(85)<223>pro、his、arg、gln或asp<220><221>misc_feature<222>(88)..(88)<223>ala或ser<220><221>misc_feature<222>(89)..(89)<223>asp或asn<220><221>misc_feature<222>(92)..(92)<223>ile或leu<220><221>misc_feature<222>(94)..(94)<223>ile、leu、thr、glu或ala<220><221>misc_feature<222>(100)..(100)<223>val或ile<220><221>misc_feature<222>(101)..(101)<223>ala或ile<220><221>misc_feature<222>(108)..(108)<223>glu或asp<220><221>misc_feature<222>(111)..(111)<223>gly或cys<220><221>misc_feature<222>(116)..(116)<223>asp或glu<220><221>misc_feature<222>(119)..(119)<223>ile或val<220><221>misc_feature<222>(127)..(127)<223>ile、leu或thr<220><221>misc_feature<222>(130)..(130)<223>ala、gly或pro<220><221>misc_feature<222>(134)..(134)<223>leu或val<220><221>misc_feature<222>(145)..(145)<223>lys或gln<220><221>misc_feature<222>(152)..(152)<223>cys、met、his、asn或thr<220><221>misc_feature<222>(154)..(154)<223>ala或gly<220><221>misc_feature<222>(162)..(162)<223>leu或ile<220><221>misc_feature<222>(166)..(166)<223>tyr或ala<220><221>misc_feature<222>(171)..(171)<223>ala或ser<220><221>misc_feature<222>(173)..(173)<223>gly或ala<220><221>misc_feature<222>(178)..(178)<223>leu、met或thr<220><221>misc_feature<222>(184)..(184)<223>leu、val、ile、met或cys<220><221>misc_feature<222>(186)..(186)<223>ala或gly<220><221>misc_feature<222>(190)..(190)<223>phe或tyr<220><221>misc_feature<222>(194)..(194)<223>asp或gly<220><221>misc_feature<222>(210)..(210)<223>glu、asn或val<220><221>misc_feature<222>(212)..(212)<223>asp或glu<220><221>misc_feature<222>(213)..(213)<223>ser或thr<220><221>misc_feature<222>(220)..(220)<223>leu或glu<220><221>misc_feature<222>(222)..(222)<223>ser或ile<220><221>misc_feature<222>(223)..(223)<223>glu、asn、his、gln或ser<220><221>misc_feature<222>(227)..(227)<223>gln、lys、thr或asp<220><221>misc_feature<222>(231)..(231)<223>asn或ile<220><221>misc_feature<222>(232)..(232)<223>ile或val<220><221>misc_feature<222>(236)..(236)<223>phe、leu<220><221>misc_feature<222>(238)..(289)<223>phe或tyr<220><221>misc_feature<222>(241)..(241)<223>thr、leu或pro<220><221>misc_feature<222>(243)..(243)<223>glu、phe、gly或pro<220><221>misc_feature<222>(244)..(244)<223>val或缺失<220><221>misc_feature<222>(245)..(245)<223>asn或asp<220><221>misc_feature<222>(249)..(249)<223>asn或asp<220><221>misc_feature<222>(250)..(250)<223>asp或glu<220><221>misc_feature<222>(251)..(251)<223>lys或asn<220><221>misc_feature<222>(254)..(254)<223>arg或ala<220><221>misc_feature<222>(268)..(268)<223>val或ala<220><221>misc_feature<222>(271)..(271)<223>met或ile<220><221>misc_feature<222>(274)..(274)<223>ala、lys或glu<220><221>misc_feature<222>(278)..(278)<223>lys、arg、glu、asp或gln<220><221>misc_feature<222>(280)..(280)<223>gln、asn、ala、his或glu<220><221>misc_feature<222>(282)..(282)<223>glu、ala或gly<220><221>misc_feature<222>(285)..(285)<223>gly、asp或asn<220><221>misc_feature<222>(296)..(296)<223>ala、val、ile或leu<220><221>misc_feature<222>(297)..(297)<223>ser或asn<220><221>misc_feature<222>(302)..(302)<223>ile或thr<220><221>misc_feature<222>(304)..(304)<223>lys、met或gln<220><221>misc_feature<222>(306)..(306)<223>val或ile<220><221>misc_feature<222>(309)..(309)<223>glu或lys<220><221>misc_feature<222>(311)..(311)<223>asn或his<220><221>misc_feature<222>(312)..(312)<223>ala或asp<220><221>misc_feature<222>(313)..(313)<223>lys、gln、glu或met<220><221>misc_feature<222>(314)..(314)<223>pro或ser<220><221>misc_feature<222>(315)..(315)<223>gly、asp、glu或lys<220><221>misc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isargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>203<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>203metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyralaglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargtyrserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnasnileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysmetile260265270thrlysglnleuglulysasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleualaproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslysarg355360365valala370<210>204<211>370<212>prt<213>人工(artificial)<220><223>突变型3-酮酰基-辅酶a合酶<400>204metglyileargilethrglythrglyleuphehisprothraspphe151015ilethrasnglugluleuvalgluserleuasnalatyrvalglugln202530tyrasnleugluasnalaasplysilealaalaglygluilegluglu354045leuargglyserseralaglupheileglulysalaserglyilelys505560argargtyrvalalaglulysthrglyileleuaspprolysargleu65707580argproleuleuhisgluargserasnaspgluleuserileglnala859095glutrpglyvalalaalaalalysglnalametgluasnalaglyval100105110thralagluaspileaspvalvalileleusercysserasnilegln115120125argalatyrproalaleualailegluileglnthralaleuglyile130135140glnglytyralatyraspmetasnvalalacysseralaalathrphe145150155160glyilelysglnalaalaaspalailelysserglyalaargargval165170175leumetvalasnvalgluileileserglyhisthrasppheargser180185190argaspcyshispheilepheglyaspvalalathralaserileile195200205glugluthrgluthrlysthrglyphegluilegluaspilegluleu210215220phethrglnpheserasnileileargasnasnpheglytyrleuasn225230235240leusergluvalaspalaaspileaspasnasnargpheargglnasp245250255glyarglysvalphelysgluvalcysproleualaalalysmetile260265270thrlysglnleuglulysasnglnilegluprothrasnvallysarg275280285phetrpleuhisglnalaasnalaasnmetasngluleuileleulys290295300leuilevalglylysgluhisalalyssergluleuvalproleuile305310315320leuaspgluphealaasnthrglyseralaglyvalileilealaleu325330335hisargthralaasngluvalasnaspglyglutyrglyvalleucys340345350serpheglyalaglytyrservalglyserileleuvallyslyshis355360365valala370当前第1页12