清洁具有包含脂肪酸的污垢的表面的方法以及用于该方法的消费产品组合物与流程

序列表的引用本申请包含计算机可读形式的序列表。所述计算机可读形式以引用方式并入本文。本发明涉及将包含脂肪酸的污垢转化为活性脂肪酸衍生物物质的方法以及包含表面活性剂和污垢转化酶的消费产品组合物。
背景技术：
：消费产品组合物，例如用于清洁表面的那些，应具有良好的起泡特征，尤其是在油脂污垢的存在下具有持久的起泡特征，同时提供良好的污垢和/或油脂清洁性。使用者通常将泡沫视为洗涤剂组合物性能的指标。此外，洗涤剂组合物的使用者还可使用起泡特征和泡沫的外观(例如密度、白度)作为洗涤溶液仍含有活性洗涤剂成分的指示剂。因此，希望洗涤剂组合物在洗涤剂与水的初始混合期间和/或整个洗涤操作期间提供“良好的起泡特征”，该起泡特征包括良好的泡沫高度和/或密度以及良好的泡沫持续时间。已经发现，某些类型的污垢，特别是包含脂肪酸的油脂污垢，可起到抑泡剂的作用，从而引发消费者比所需的更频繁地更换产品。因此，需要提供具有所需泡沫性能，尤其是在油脂污垢存在下，甚至是在包含脂肪酸的油脂污垢存在下，并且同时提供良好的污垢和油脂去除性质的洗涤剂组合物。还期望在消费产品组合物中使用较少的表面活性剂物质。然而，使用较少的表面活性剂可能会降低消费产品组合物的泡沫生成和/或清洁性能。仍然希望提供用于清洁具有包含脂肪酸的污垢的表面的消费产品组合物，该消费产品组合物提供有效的泡沫生成和/或清洁性能，尤其是当消费产品组合物在组合物中包含相对低量的表面活性剂时。技术实现要素：本发明涉及一种清洁上面存在包含脂肪酸的污垢的表面的方法，该方法包括使该污垢与消费产品组合物接触，该消费产品组合物包含表面活性剂和污垢转化酶，该污垢转化酶选自氢过氧脂肪酸生成酶、氢过氧脂肪酸转化酶、多结构域酶、羟基脂肪酸生成酶、以及它们的混合物。该方法还包括将污垢的脂肪酸转化为选自氢过氧脂肪酸、氢过氧脂肪酸衍生物、羟基脂肪酸、以及它们的混合物的活性脂肪酸衍生物物质。本发明还涉及消费产品组合物，该消费产品组合物包含：表面活性剂，该表面活性剂选自阴离子表面活性剂、非离子表面活性剂、两性离子表面活性剂、两性表面活性剂、阳离子表面活性剂、以及它们的混合物；和污垢转化酶，该污垢转化酶选自氢过氧脂肪酸生成酶、氢过氧脂肪酸转化酶、多结构域酶、羟基脂肪酸生成酶、以及它们的混合物。具体实施方式定义如本文所用，当用于权利要求中时，冠词“一个”和“一种”被理解为是指受权利要求书保护或描述的一种或多种。如本文所用，术语“大体上不含”是指按组合物的重量计，所指示的物质以不超过约5重量％，优选不超过约2％，并且更优选不超过约1重量％的量存在。如本文所用，术语“基本不含”是指按组合物的重量计，所指示的物质以不超过约0.1重量％的量存在，或优选地不以分析上可检测的水平存在于此组合物中。其可包括这样的组合物，其中所指示的物质仅作为有意加入此类组合物中的一种或多种物质的杂质。如本文所用，术语“洗涤剂组合物”是指设计用于清洁脏污表面的组合物或制剂。此类组合物包括但不限于盘碟洗涤组合物、衣物洗涤剂组合物、织物软化组合物、织物增强组合物、织物清新组合物、衣物洗涤预洗剂、衣物洗涤预处理剂、衣物洗涤添加剂、喷雾产品、干洗剂或组合物、衣物洗涤冲洗添加剂、洗涤添加剂、后冲洗织物处理剂、熨烫助剂、硬质表面清洁组合物、单位剂量制剂、延迟递送制剂、在多孔基底或非织造片材上或之内包含的洗涤剂、以及根据本文的教导内容对本领域的技术人员显而易见的其他合适的形式。此类组合物可被用作清洁预处理剂、清洁后处理剂，或也可在清洁过程的冲洗循环或洗涤循环期间添加。洗涤剂组合物可具有选自以下的形式：液体、粉末、单相或多相单位剂量或小袋形式、片剂、凝胶、糊剂、棒或薄片。优选地，组合物用于手动洗涤。优选地，本发明的洗涤剂组合物为盘碟洗涤剂。优选地，组合物为液体形式。如本文所用，术语“片段”是指参考序列的至少30、60、100、150(或其间的任何整数)个连续氨基酸的氨基酸序列。本文所用术语“同一性”是指两个或更多个序列之间的同一性，并且以序列之间的同一性或相似性来表示，该同一性或相似性如通过将序列的全长与参考序列的全长进行比对来计算。序列同一性可以同一性百分比来量度；百分比越高，序列同一性越高。同一性百分比通过比较的长度来计算。例如，通常通过将序列的全长与参考序列的全长进行比对来计算同一性。用于比较的序列比对方法是本领域熟知的，并且同一性可以通过许多已知方法来计算。本领域中描述了各种程序和比对算法。应当注意，术语“序列同一性”和“序列相似性”可以互换使用。如本文所用，术语“增加的泡沫寿命”是指在使用包含一种或多种氢过氧脂肪酸生成酶和一种或多种氢过氧脂肪酸转化酶的组合物清洁脏污制品的洗涤过程中，与不存在氢过氧脂肪酸生成酶和氢过氧脂肪酸转化酶的情况下由相同组合物和方法提供的泡沫寿命相比，可见泡沫的持续时间增加。如本文所用，术语“脏污表面”非特定地指任何类型的由天然纤维或人造纤维的网络构成的柔性材料，包括天然纤维、人造纤维和合成纤维，诸如但不限于棉、亚麻布、羊毛、聚酯、尼龙、丝绸、丙烯酸类等、以及各种共混物和组合。脏污表面还可指任何类型的硬质表面，包括天然表面、人造表面或合成表面，诸如但不限于砖材、花岗石、灰浆、玻璃、复合材料、乙烯树脂、硬木、金属、烹饪表面、塑料等、以及共混物和组合。本申请的主要目标脏污表面是脏污盘碟。如本文所用，术语氢过氧脂肪酸生成酶或氢过氧脂肪酸转化酶的“变体”是指当氢过氧脂肪酸生成酶或氢过氧脂肪酸转化酶被选自取代、插入、缺失及其组合的一个或多个氨基酸修饰或在这些氨基酸处被修饰(例如1、2、3、4、5、6、7、8、9或10个或更多个氨基酸修饰)时的氨基酸序列。变体可以具有“保守”取代，其中被取代的氨基酸具有与取代它的氨基酸相似的结构或化学性质，例如用异亮氨酸替代亮氨酸。变体可以具有“非保守”改变，例如，用色氨酸替代甘氨酸。变体还可以包括具有氨基酸缺失或插入或两者的序列。使用本领域熟知的计算机程序可以找到确定哪些氨基酸残基可以被取代、插入或缺失而不消除蛋白质活性的指导。变体还可以包括衍生自野生型氢过氧脂肪酸生成酶或氢过氧脂肪酸转化酶的截短形式，例如具有截短的n-末端的蛋白质。变体还可以包括通过向野生型蛋白质添加额外的氨基酸序列，例如n-末端标签、c-末端标签或在蛋白序列中间的插入物而衍生的形式。如本文所用，术语“水硬度”或“硬度”是指存在于水中的未络合阳离子(即，ca2+或mg2+)，这些阳离子在碱性条件下可能与阴离子表面活性剂或任何其他带阴离子电荷的洗涤剂活性物质一起沉淀，从而削弱表面活性剂的表面活性和清洁能力。另外，术语“高水硬度”或“提升的水硬度”可互换使用，并且出于本发明的目的，是相关术语，并且旨在包括但不限于包含至少12克钙离子每加仑水(gpg，“美国格令硬度”单位)的硬度水平。消费产品组合物本发明涉及一种包含表面活性剂和污垢转化酶的消费产品组合物。当用于接触上面存在包含脂肪酸的污垢的脏污表面时，所述消费产品组合物可将污垢的脂肪酸转化为活性脂肪酸衍生物物质。就这一点而言，在消费产品组合物中使用较低含量的表面活性剂时，本发明的消费产品组合物可表现出改善的清洁性能，或等同的清洁性能。本发明的消费产品组合物包括但不限于用于下列的组合物：处理毛发(人类、狗和/或猫)，包括漂白、着色、染色、调理、生长、除去、延迟生长、用洗发剂洗发、定型；除臭剂和止汗剂；个人清洁；与处理皮肤(人、犬和/或猫)相关的产品和/或方法，所述处理包括施用乳霜、洗剂、以及消费者使用的其他局部施用的产品；剃刮；身体喷雾剂；用于处理织物、硬质表面以及任何其他在织物和居家护理领域中的表面的组合物，包括：空气护理、汽车护理、餐具洗涤、织物调理(包括软化)、衣物洗涤去垢、衣物洗涤和冲洗添加剂和/或护理、硬质表面清洁和/或处理、和用于消费者或企业使用的其他清洁；掺入与一次性吸收制品和/或非吸收制品相关的产品中的组合物，这些产品包括成人失禁衣服、围兜、尿布、训练裤、婴儿和幼儿护理擦拭物；洗手液、洗发剂、洗剂、口腔护理工具；诸如湿或干的卫生纸、面巾纸、一次性手帕、一次性毛巾和/或擦拭物的产品；掺入与月经垫、失禁衬垫、阴唇间衬垫、卫生护垫、阴道栓剂、卫生巾、棉塞和棉塞施用装置和/或擦拭物的产品中的组合物。在优选的方面，消费产品组合物为洗涤剂组合物。本文中优选的消费产品组合物包括织物清洁组合物、硬表面清洁组合物、盘碟洗涤组合物和毛发清洁组合物。此类组合物通常包含一种或多种消费产品助剂成分。优选的消费产品组合物为手动盘碟洗涤组合物，优选液体形式。其通常包含按所述组合物的重量计30％至95％，优选40％至90％，更优选50％至85％的液体载体，其他必要组分和任选组分溶解、分散或悬浮在所述液体载体中。液体载体的一个优选组分是水。优选地，将本发明的消费产品组合物的ph调节至在3至14之间，更优选在4至13之间，更优选在6至12之间，并且最优选在8至10之间，所述ph在20℃下以在去离子水中的10％产品浓度测量。可使用本领域已知的ph调节成分来调节消费产品组合物的ph。污垢转化酶消费产品组合物包含：a)一种或多种污垢转化酶，其选自氢过氧脂肪酸生成酶、氢过氧脂肪酸转化酶、氢过氧脂肪酸生成和转化酶、过加氧酶、脂肪酸水合酶、以及它们的混合物；和b)表面活性剂。所述消费产品组合物还可包含如本文所述的消费产品助剂成分。在一个方面，所述消费产品组合物中表面活性剂的含量按所述组合物的重量计小于约2％，优选小于约1％，优选小于约0.5％，优选小于约0.1％，或优选0％，同时通过将油性和/或油脂污垢转化为表面活性剂而仍然提供增强的对此类污垢的起泡和/或清洁有益效果。据信，起泡和/或清洁有益效果的增加是由于脂肪酸转化为更含氧的脂肪酸，表面活性剂性质增强，并且在硬水存在下沉淀的趋势减小。此外，据信表面活性剂的浓度按所述组合物的重量计低于约2％可有利于消费产品组合物中酶的稳定性和活性。氢过氧脂肪酸生成酶在分子氧(o2)存在下脂肪酸可以被氢过氧脂肪酸生成酶如双加氧酶氧化，以产生氢过氧脂肪酸。这些氢过氧化化合物可进一步被酶促处理或自发地转化为不同组的含氧脂肪酸和其他衍生物。在本申请的上下文中，“氢过氧脂肪酸生成酶”是能够将至少一种脂肪酸转化为含氧化合物的混合物的酶，该混合物至少包含作为中间体或作为最终产物的氢过氧脂肪酸。氢过氧脂肪酸生成酶的非限制性实例为脂氧合酶、α-双加氧酶、二醇合酶和含有具有双加氧酶(dox)活性的结构域的其他酶。在本发明的一个实施方案中，所述清洁组合物包含脂氧合酶。脂氧合酶(ec1.13.11.-)是一类(非血红素)、含铁或锰的双加氧酶(dox)，其催化分子氧插入不饱和脂肪酸中以生成相应的氢过氧脂肪酸。本发明包括不同组的脂氧合酶，包括亚油酸脂氧合酶、花生四烯酸脂氧合酶和油酸脂氧合酶。尽管亚油酸脂氧合酶、花生四烯酸脂氧合酶和油酸脂氧合酶通常分别识别亚油酸/亚油酸酯、花生四烯酸/花生四烯酸酯以及油酸/油酸酯作为优选底物，但术语“亚油酸脂氧合酶”、“花生四烯酸脂氧合酶”和“油酸脂氧合酶”在本文中可互换使用，且不暗示任何底物特异性，即，相应的酶可作用于这些底物中的任一种。区域特异性脂氧合酶催化不饱和脂肪酸的位置特异性氢过氧化。例如，动物12-和15-脂氧合酶和微生物15-脂氧合酶将花生四烯酸转化为对应的12-和15-氢过氧脂肪酸；而来自珊瑚和海胆的11-脂氧合酶产生11-氢过氧脂肪酸作为中间体或最终产物。类似地，植物和细菌的9-和13-脂氧合酶和真菌11-和13-脂氧合酶将亚油酸转化为其9-、11-和13-氢过氧脂肪酸衍生物。此外，一些双加氧酶能够催化分子氧在不饱和脂肪酸的若干位置处的结合。作为本发明一部分的脂氧合酶的非限制性实例包括表1中所列的野生型脂氧合酶及其表现出脂氧合酶活性的变体。优选的脂氧合酶表现出与表1中所列野生型脂氧合酶中的一种或多种至少20％，优选至少30％，优选至少40％，优选至少50％，优选至少60％，优选至少70％，优选至少80％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性。表1：脂氧合酶在本发明的另一个实施方案中，所述清洁组合物包含α-双加氧酶。α-双加氧酶是另一类双加氧酶(dox)，其通过立体选择性双加氧作用将饱和与不饱和脂肪酸转化为其对应的2-氢过氧脂肪酸。所得氢过氧脂肪酸可以自发脱羧为较短的醛。α-双加氧酶与脂氧合酶的不同之处在于在氧化过程中不需要不饱和碳键。α-双加氧酶通常由不同种类的植物和真菌编码，其中它们在针对病原体攻击的宿主防御反应期间被上调，但在细菌中也发现同源物。作为本发明一部分的α-双加氧酶的非限制性实例包括表2中所列的野生型及其表现出α-双加氧酶活性的变体。优选的α-双加氧酶表现出至少20％，优选至少30％，优选至少40％，优选至少50％，优选至少60％，优选至少70％，优选至少80％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性，如通过将序列的全长与选自表2中所列野生型α-双加氧酶的至少一个参考序列的全长进行比对所计算的。表2：α-双加氧酶氢过氧脂肪酸转化酶氢过氧脂肪酸可被催化或自发地转化为含氧衍生物，包括羟基-、二羟基-、氧代-、环氧-和酮脂肪酸、二乙烯基醚和醛(andreou,a.等人(2009)，prog.lipidres.48(3-4):148-170)。本发明的清洁组合物还可包含一种或多种氢过氧脂肪酸转化酶，所述氢过氧脂肪酸转化酶能够将氢过氧脂肪酸转化为氢过氧脂肪酸的一种或多种衍生物。优选地，氢过氧脂肪酸转化酶选自：环加氧酶(ec1.14.99.1)、丙二烯氧化物合酶(ec4.2.1.92)、氢过氧化物异构酶(ec4.2.1.92、ec5.3.99.1、ec5.4.4.5、ec5.4.4.6)、氢过氧化物裂解酶(ec4.2.1.92)、氢过氧化物脱水酶(ec4.2.1.92)、二乙烯基醚合酶(ec4.2.1.121、ec4.2.1.b8、ec4.2.1.b9)、9,12-十八碳二烯酸8-氢过氧化物8r-异构酶(ec5.4.4.5)、9,12-十八碳二烯酸8-氢过氧化物8s-异构酶(ec5.4.4.6)、7,10-氢过氧化物二醇合酶、环氧醇合酶、以及它们的混合物。优选地，氢过氧脂肪酸转化酶选自：氢过氧化物异构酶(ec4.2.1.92、ec5.3.99.1、ec5.4.4.5、ec5.4.4.6)、氢过氧化物裂解酶(ec4.2.1.92)、氢过氧化物脱水酶(ec4.2.1.92)、7,10-氢过氧化物二醇合酶、环氧醇合酶、以及它们的混合物，优选7,10-氢过氧化物二醇合酶。氢过氧脂肪酸转化酶的非限制性实例包括表2中列出的野生型及其表现出氢过氧脂肪酸转化酶活性的变体。优选的氢过氧脂肪酸转化酶表现出与表3中所列野生型中的一种或多种至少20％，优选至少30％，优选至少40％，优选至少50％，优选至少60％，优选至少70％，优选至少80％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性。优选的氢过氧脂肪酸转化酶也可以是表3中所列野生型的片段(例如n-末端结构域或c-末端结构域)。表3：氢过氧脂肪酸转化酶由氢过氧脂肪酸转化酶转化氢过氧脂肪酸而形成的氢过氧脂肪酸衍生物优选选自二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇、以及它们的混合物，优选二羟基脂肪酸。多结构域酶在本发明的另一个实施方案中，至少一种氢过氧脂肪酸生成酶和至少一种氢过氧脂肪酸转化酶在多肽链中连接在一起。在另一个实施方案中，所述清洁组合物包含含有多肽结构域的一条或多条多结构域多肽链，所述多肽结构域包括：a)氢过氧脂肪酸生成结构域，和b)氢过氧脂肪酸转化结构域，优选地所述氢过氧脂肪酸生成结构域是双加氧酶(dox)结构域，并且优选地所述氢过氧脂肪酸转化结构域选自丙二烯氧化物合酶(aos)结构域、环氧醇合酶(eas)结构域、氢过氧化物裂解酶(hpl)结构域和氢过氧化物异构酶(hpi)结构域。在反应的第一步中，这些酶的dox结构域将不饱和脂肪酸转化为氢过氧脂肪酸，随后往往进行由hpi、aos或eas结构域催化的另外的转化。据信当两种酶连接在一起时，转化速率和对氢过氧脂肪酸的期望衍生物的选择性可得到增强。包括a)氢过氧脂肪酸生成结构域和b)氢过氧脂肪酸转化结构域的多结构域酶的若干实例存在于自然界中。本发明包括的这些多结构域酶的实例为二醇合酶。在本申请的上下文中，“二醇合酶”是能够将至少一种不饱和脂肪酸转化为包含至少二羟基脂肪酸的氧脂素混合物的酶。本领域已经报道了至少两种不同类的二醇合酶。i类真菌二醇合酶，也称为psi因子生成加氧酶(ppo)，其包含n-末端双加氧酶(dox)结构域和c-末端细胞色素p450/氢过氧化物异构酶(hpi)结构域；而ii类细菌二醇合酶由n-末端丙二烯氧化物合酶(aos)结构域和c-末端双加氧酶(dox)结构域组成。在反应的第一步中，不饱和脂肪酸(例如亚油酸)通过dox结构域被转化为氢过氧脂肪酸衍生物，随后往往通过hpi结构域或aos结构域被异构化为二羟基脂肪酸。若干个氨基酸残基在i类二醇合酶中是保守的。例如，在dox结构域中，含有催化性tyr的yr(w/f)h基序是高度保守的。在hpi结构域中，srs-4区域基序anqxq、exxg基序和血红素特征基序(g/e)(p/a)hx(c/s)(l/f/g)也经常存在于二醇合酶中。血红素基序的his残基和cys残基以及srs-4区的最后一个asn与异构化步骤和反应期间产生的氧脂素的类型有关。本发明包含不同组的二醇合酶，包括亚油酸二醇合酶和油酸二醇合酶。尽管油酸二醇合酶通常将油酸/油酸酯识别为优选的底物，亚油酸二醇合酶将亚油酸/亚油酸酯识别为优选的底物，但术语“油酸二醇合酶”和“亚油酸二醇合酶”在本文中可互换使用并且不暗示任何底物特异性，即，各自的酶可作用于两种底物。基于反应产物，已表征了若干种二醇合酶：5,8-亚油酸二醇合酶(5,8-lds)、7,8-亚油酸二醇合酶(7,8-lds)、8,11-亚油酸二醇合酶(8,11-lds)和9,14-亚油酸二醇合酶(9,14-lds)。尽管它们经常被称为亚油酸二醇合酶，但是它们可以转化不同于亚油酸酯(例如油酸酯)的底物。本发明包括的二醇合酶的非限制性实例列于表4中。例如，构巢曲霉ppoa(seqidno:56)将c16和c18不饱和脂肪酸(包括棕榈油酸、油酸、亚油酸和α-亚麻酸)转化成5,8-二羟基脂肪酸，并将c20不饱和脂肪酸(包括二十碳烯酸、二十碳二烯酸和二十碳三烯酸)转化成7,10-二羟基脂肪酸(brodhun,f.等人(2009),j.biol.chem.284(18):11792-11805，以及jerneren,f.等人(2010),biochim.biophys.acta,mol.cellbiol.lipids1801(4):503-507)。在另一个实例中，小麦全蚀病菌7,8-lds(seqidno:67)将油酸、亚油酸和α-亚麻酸转化为7,8-二羟基脂肪酸作为主要产物，但当γ-亚麻酸、二十碳三烯酸、花生四烯酸和二十碳五烯酸用作底物时，该酶不显示活性。类似地，苹果炭疽病菌7,8-lds(seqidno:66)将棕榈油酸、油酸、亚油酸和α-亚麻酸转化为7,8-二羟基脂肪酸，而且通过仅使用n-末端双加氧酶结构域将二十碳烯酸、二十碳二烯酸、二高-γ-亚麻酸和花生四烯酸加工成8-氢过氧脂肪酸(seo,m.-j.等人(2016),appl.microbiol.biotechnol.100(7):3087-3099)。此外，产黄青霉8,11-lds(seqidno:70)将亚油酸和α-亚麻酸转化为8,11-二羟基脂肪酸，而油酸和棕榈油酸转化为8-羟基脂肪酸。有意思的是，苹果炭疽病菌7,8-lds的q898e或q898l变体也将亚油酸转化为8,11-二羟基脂肪酸(shin,k.-c.等人(2016),j.lipidres.57(2):207-218)。念珠藻属pcc71209,14-lds(seqidno:2)将亚油酸与9,10-二羟基、8-11-二羟基和9-羟基脂肪酸转化为主产物9,14-二羟基脂肪酸；将α-亚麻酸转化为9,16-二羟基、9,13-二羟基和9-羟基脂肪酸；并且将γ-亚麻酸转化为9,14-二羟基和9-羟基脂肪酸(lang,i.等人(2008),biochem.j.410(2):347-357)。表4：二醇合酶在自然界中已经观察到其他二羟基化模式，但是酶还没有被表征。例如，巨大芽孢杆菌(bacillusmegaterium)ala2和棍状杆菌属(clavibactersp.)ala2从亚油酸产生12,13-二羟基脂肪酸。在另一个实例中，红藻龙须菜(gracilariopsislemaneiformis)从花生四烯酸产生9,10-二羟基、12,12-二羟基和12-羟基脂肪酸。最后，水节霉(leptomituslacteus)将亚油酸转化为8,11-二羟基、11,16-二羟基、11,17-二羟基、7-羟基和8-羟基脂肪酸。因此，二醇合酶可用于将不饱和脂肪酸转化为不同的羟基化产物(kim,k.-r.和d.-k.oh(2013),biotechnol.adv.31(8):1473-1485)。在另一个实施方案中，二醇合酶选自：亚油酸二醇合酶(ec1.13.11.44)、5,8-亚油酸二醇合酶(ec1.13.11.60和ec5.4.4.5)、7,8-亚油酸二醇合酶(ec1.13.11.60和ec5.4.4.6)、9,14-亚油酸二醇合酶(ec1.13.11.b1)、8,11-亚油酸二醇合酶、油酸二醇合酶、以及它们的混合物。在另一个实施方案中，二醇合酶与选自以下的野生型二醇合酶中的一种或多种具有至少60％，优选至少70％，优选至少80％，优选至少85％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性：构巢曲霉ppoa(seqidno:56)、烟曲霉ppoa(seqidno:61)、土曲霉ppoa(seqidno:62)、白曲霉ppoa(seqidno:63)、棒曲霉ppoa(seqidno:64)、黑曲霉ppoa(seqidno:65)、苹果炭疽病菌7,8-lds(seqidno:66)、小麦全蚀病菌7,8-lds(seqidno:67)、稻瘟病菌7,8-lds(seqidno:68)、点形念珠藻8,11-lds(seqidno:69)、产黄青霉8,11-lds(seqidno:70)、指状青霉8,11-lds(seqidno:71)、念珠藻属pcc71209,14-lds(seqidno:2)、推定的深海单细胞蓝细菌9,14-lds(seqidno:72)和推定的念珠藻属nies-41039,14-lds(seqidno:73)。优选地，二醇合酶是5,8-亚油酸二醇合酶(ec1.13.11.60和ec5.4.4.5)。优选地，5,8-亚油酸二醇合酶(ec1.13.11.60和ec5.4.4.5)与选自以下的野生型二醇合酶中的一种或多种具有至少60％，优选至少70％，优选至少80％，优选至少85％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性：构巢曲霉ppoa(seqidno:56)、烟曲霉ppoa(seqidno:61)、土曲霉ppoa(seqidno:62)、白曲霉ppoa(seqidno:63)、棒曲霉ppoa(seqidno:64)和黑曲霉ppoa(seqidno:65)、以及它们的混合物，更优选构巢曲霉ppoa(seqidno:56)。在包含a)氢过氧脂肪酸生成结构域和b)氢过氧脂肪酸转化结构域的多结构域酶的另一个实例中，含有双加氧酶(dox)结构域和丙二烯氧化物合酶(aos)结构域的酶产生一系列不同的不饱和脂肪酸的含氧衍生物并且包括在本发明中。这些dox-aos酶的非限制性实例列于表5中。在另一个实例中，酶可以含有双加氧酶(dox)结构域和环氧醇合酶(eas)结构域并且也包括在本发明中。。这些dox-eas酶的非限制性实例列于表5中。表5：其他多结构域酶优选地，基于组合物中的活性蛋白质，氢过氧脂肪酸生成酶以组合物重量的0.0001重量％至1重量％的量存在。更优选地，基于组合物中的活性蛋白质，氢过氧脂肪酸生成酶以组合物重量的0.001重量％至0.2重量％的量存在。在本发明的一个实施方案中，通过氢过氧脂肪酸生成酶转化的脂肪酸选自：单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、饱和脂肪酸、以及它们的混合物；优选肉豆蔻油酸、肉豆蔻酸、十五烷酸、棕榈油酸、棕榈酸、十六碳烯酸(sapienicacid)、十七烷酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、硬脂酸、鳕油酸、花生酸、山萮酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物，优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物，更优选油酸。在另一个实施方案中，由脂肪酸与氢过氧脂肪酸生成酶的转化反应所形成的所得氢过氧脂肪酸选自2-氢过氧脂肪酸、8r-氢过氧脂肪酸、8s-氢过氧脂肪酸、9r-氢过氧脂肪酸、9s-氢过氧脂肪酸、10r-氢过氧脂肪酸、11r-氢过氧脂肪酸、11s-氢过氧脂肪酸、12r-氢过氧脂肪酸、12s-氢过氧脂肪酸、13r-氢过氧脂肪酸、13s-氢过氧脂肪酸、14r-氢过氧脂肪酸、14s-氢过氧脂肪酸、15s-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；优选2r-氢过氧脂肪酸、不饱和5s-氢过氧脂肪酸、不饱和8r-氢过氧脂肪酸、不饱和9r-氢过氧脂肪酸、不饱和11r-氢过氧脂肪酸、不饱和12r-氢过氧脂肪酸、不饱和12s-氢过氧脂肪酸、不饱和13s-氢过氧脂肪酸、不饱和15s-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；更优选2r-氢过氧脂肪酸。所得氢过氧脂肪酸可自发或酶促转化为单羟基脂肪酸、二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇或其他衍生物。单羟基脂肪酸的非限制性实例为2-羟基脂肪酸、不饱和5-羟基脂肪酸、不饱和8-羟基脂肪酸、不饱和9-羟基脂肪酸、不饱和11-羟基脂肪酸、不饱和12-羟基脂肪酸、不饱和13-羟基脂肪酸、不饱和15-羟基脂肪酸、它们的衍生物、以及它们的混合物。二羟基脂肪酸的非限制性实例为5,8-二羟基脂肪酸、7,8-二羟基脂肪酸、7,10-二羟基脂肪酸、8,11-二羟基脂肪酸、9,14-二羟基脂肪酸、以及它们的混合物。醛的非限制性实例为1-链烷醛、链烯-1-醛、链二烯-1-醛、链三烯-1-醛、链四烯-1-醛、以及它们的混合物。羟基脂肪酸生成酶本发明的污垢转化酶可以是羟基脂肪酸生成酶，优选选自过加氧酶、脂肪酸水合酶、以及它们的混合物。过加氧酶脂肪酸可以在过氧化氢(h2o2)存在下通过过加氧酶而氧化，从而生成羟基脂肪酸。在本发明的另一个实施方案中，所述清洁组合物包含一种或多种酶，所述一种或多种酶选自：非特异性过加氧酶(ec1.11.2.1)、植物种子过加氧酶(ec1.11.2.3)、脂肪酸过加氧酶(ec1.11.2.4)、以及它们的混合物。过加氧酶，也称为氢过氧化物氧化还原酶，可以使用过氧化氢作为共底物催化脂肪酸转化为羟基化衍生物。本发明包括的过加氧酶的非限制性实例列于表6中。例如，少动鞘氨醇单胞菌(sphingomonaspaucimobilis)过加氧酶催化脂肪酸在α-位的羟基化，而枯草芽孢杆菌过加氧酶催化α-位(40％)和β-位(60％)处的羟基化。表6：过加氧酶在本发明的一个实施方案中，通过过加氧酶转化的脂肪酸选自单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、饱和脂肪酸、以及它们的混合物；优选肉豆蔻油酸、肉豆蔻酸、十五烷酸、棕榈油酸、棕榈酸、十六碳烯酸、十七烷酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、硬脂酸、鳕油酸、花生酸、山萮酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物，优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物，更优选油酸。在另一个实施方案中，由脂肪酸与过加氧酶的转化反应所形成的所得羟基脂肪酸选自2-羟基脂肪酸、3-羟基脂肪酸、4-羟基脂肪酸、5-羟基脂肪酸、6-羟基脂肪酸、以及它们的混合物；优选2-羟基脂肪酸、3-羟基脂肪酸、以及它们的混合物。脂肪酸水合酶不饱和脂肪酸可以在水(h2o)存在下通过脂肪酸水合酶而水合，从而生成羟基脂肪酸。在另一个实施方案中，所述清洁组合物包含一种或多种脂肪酸水合酶(ec4.2.1.53)。脂肪酸水合酶催化水对不饱和脂肪酸双键的区域特异性且不可逆加成，从而生成羟基脂肪酸。不饱和脂肪酸的水合在细菌中是常见的。例如，还原亚硝酸盐寡养单胞菌(stenotrophomonasnitritireducens)在c-10位处对亚油酸进行水合，而鹑鸡肠球菌(enterococcusgallinarum)、黄杆菌属(flavobacteriumsp.)、乳杆菌属(lactobacillussp.)、乳酸片球菌(pediococcusacidilactici)和反刍动物月形单胞菌(selenomonasruminantium)对亚油酸进行水合。油酸水合酶可以将顺-9和顺-12不饱和脂肪酸转化为10-或13-羟基脂肪酸或10,13-二羟基脂肪酸。油酸水合酶的非限制性实例列于表7中。表7：脂肪酸水合酶在本发明的一个实施方案中，通过水合酶转化的脂肪酸选自单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、以及它们的混合物；优选肉豆蔻油酸、棕榈油酸、十六碳烯酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、鳕油酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物；优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物；更优选油酸。在另一个实施方案中，由脂肪酸与水合酶的转化反应所形成的所得羟基脂肪酸选自10-羟基脂肪酸、13-羟基脂肪酸、10,13-二羟基脂肪酸、以及它们的混合物。附加的考虑本发明还包括酶的变体。如本文所用，酶的变体包括当相应蛋白质的野生型蛋白质被一个或多个氨基酸(例如1、2、5或10个氨基酸)修饰或在所述一个或多个氨基酸处被修饰时产生的序列。本发明还包括来源于野生型酶的截短形式的变体，例如具有截短的n-末端或截短的c-末端的蛋白质。一些酶可以包括在被细胞分泌时可能被除去的n-末端信号肽。本发明包括没有n-末端信号肽的变体。生物信息学工具，例如signalpver4.1(petersentn.,brunaks.,vonheijneg.和nielsenh.(2011),naturemethods,8:785-786)可用于预测这类信号肽的存在和长度。本发明还包括通过向野生型蛋白添加额外的氨基酸序列，例如n-末端标签、c-末端标签或在蛋白序列中间的插入物而衍生的变体。标签的非限制性实例为麦芽糖结合蛋白(mbp)标签、谷胱甘肽s-转移酶(gst)标签、硫氧还蛋白(trx)标签、his-标签和本领域的技术人员已知的任何其他标签。标签可用于在发酵期间改善溶解度和表达水平或作为酶纯化的柄。重要的是酶的变体保留并优选地改善野生型蛋白质催化不饱和脂肪酸转化的能力。当然，可以容许变体的给定性质的一些性能下降，但是变体应当保持并且优选地改善它们所针对的相关应用的合适性质。筛选野生型之一的变体可用于鉴定它们是否保留并优选改善适当的性质。变体可以具有“保守”取代。保守取代的合适实例包括酶中的一个保守取代，例如以下序列中的保守取代：seqidno:1、seqidno:2、seqidno:3、seqidno:4、seqidno:5、seqidno:6、seqidno:7、seqidno:8、seqidno:9、seqidno:10、seqidno:11、seqidno:12、seqidno:13、seqidno:14、seqidno:15、seqidno:16、seqidno:17、seqidno:18、seqidno:19、seqidno:20、seqidno:21、seqidno:22、seqidno:23、seqidno:24、seqidno:25、seqidno:26、seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32、seqidno:33、seqidno:34、seqidno:35、seqidno:36、seqidno:37、seqidno:38、seqidno:39、seqidno:40、seqidno:41、seqidno:42、seqidno:43、seqidno:44、seqidno:45、seqidno:46、seqidno:47、seqidno:48、seqidno:49、seqidno:50、seqidno:51、seqidno:52、seqidno:53、seqidno:54、seqidno:55、seqidno:56、seqidno:57、seqidno:58、seqidno:59、seqidno:60、seqidno:61、seqidno:62、seqidno:63、seqidno:64、seqidno:65、seqidno:66、seqidno:67、seqidno:68、seqidno:69、seqidno:70、seqidno:71、seqidno:72、seqidno:73、seqidno:74、seqidno:75、seqidno:76、seqidno:77、seqidno:78、seqidno:79或seqidno:80。其他合适的实例包括蛋白质中10个或更少(例如五个或更少)的保守取代。因此，本发明的酶可以包括1、2、3、4、5、6、7、8、9、10或更多个保守取代。含有一个或多个保守取代的酶可以如下方式产生：通过使用例如标准方法，例如定点诱变或pcr，对编码该酶的核苷酸序列进行操纵。可以取代酶中原始氨基酸并被认为是保守取代的氨基酸的实例包括：ser取代ala；lys取代arg；gln或his取代asn；glu取代asp；asn取代gln；asp取代glu；pro取代gly；asn或gln取代his；leu或val取代ile；ile或val取代leu；arg或gln取代lys；leu或ile取代met；met、leu或tyr取代phe；thr取代ser；ser取代thr；tyr取代trp；trp或phe取代tyr；以及ile或leu取代val。变体包括“经修饰的酶”或“突变酶”，其包括具有至少一个氨基酸取代、插入和/或缺失的蛋白质。经修饰的酶可具有1、2、3、4、5、6、7、8、9或10或更多个氨基酸修饰(选自取代、插入、缺失及其组合)。酶可以通过多种化学技术修饰，以产生具有与未经修饰的酶基本上相同或优选改善的活性，并任选具有其他所需性质的衍生物。例如，蛋白质的羧酸基团，无论是羧基末端还是侧链，可以以药学上可接受的阳离子盐的形式提供或酯化，例如以形成c1-c6烷基酯，或转化为酰胺，例如式conr1r2的酰胺，其中r1和r2各自独立地为h或c1-c6烷基，或组合成杂环，例如5-或6-元环。酶的氨基基团，无论是氨基末端还是侧链，可以是药学上可接受的酸加成盐形式，例如hci、hbr、乙酸、苯甲酸、甲苯磺酸、马来酸、酒石酸和其他有机盐，或者可以被修饰成c1-c20烷基或二烷基氨基或进一步转化为酰胺。蛋白质侧链的羟基基团可以使用公知的技术转化为烷氧基或酯基，例如c1-c20烷氧基或c1-c20烷基酯。蛋白质侧链的苯基环和酚环可以被一个或多个卤素原子(诸如f、cl、br或i)，或被c1-c20烷基、c1-c20烷氧基、羧酸及其酯，或此类羧酸的酰胺取代。蛋白质侧链的亚甲基基团可以延伸到同源c2-c4亚烷基。硫醇可以用许多公知的保护基中的任何一种(例如乙酰胺基团)保护。本领域的技术人员还将认识到用于将环状结构引入本公开的蛋白质中以选择并提供对结构的构象限制从而导致增强的稳定性的方法。如本文所提及，关于本发明的同一性或同源性百分比是可以用gap程序计算的那些，该gap程序可从gcg(geneticscomputergroupinc.,madison,wl,usa)获得。另选地，可以执行手动比对。对于酶序列比较，可以使用以下设置：比对算法：needleman和wunsch,j.mol.biol.1970,48:443-453。使用blosum62矩阵作为氨基酸相似性的比较矩阵(henikoffs.和henikoffj.g.,p.n.a.s.usa1992,89:10915-10919)。使用以下空位评分参数：空位罚分：12，空位长度罚分：2，末端空位无罚分。通常将给定序列与下列的全长序列进行比较，以得到分数：seqidno:1、seqidno:2、seqidno:3、seqidno:4、seqidno:5、seqidno:6、seqidno:7、seqidno:8、seqidno:9、seqidno:10、seqidno:11、seqidno:12、seqidno:13、seqidno:14、seqidno:15、seqidno:16、seqidno:17、seqidno:18、seqidno:19、seqidno:20、seqidno:21、seqidno:22、seqidno:23、seqidno:24、seqidno:25、seqidno:26、seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32、seqidno:33、seqidno:34、seqidno:35、seqidno:36、seqidno:37、seqidno:38、seqidno:39、seqidno:40、seqidno:41、seqidno:42、seqidno:43、seqidno:44、seqidno:45、seqidno:46、seqidno:47、seqidno:48、seqidno:49、seqidno:50、seqidno:51、seqidno:52、seqidno:53、seqidno:54、seqidno:55、seqidno:56、seqidno:57、seqidno:58、seqidno:59、seqidno:60、seqidno:61、seqidno:62、seqidno:63、seqidno:64、seqidno:65、seqidno:66、seqidno:67、seqidno:68、seqidno:69、seqidno:70、seqidno:71、seqidno:72、seqidno:73、seqidno:74、seqidno:75、seqidno:76、seqidno:77、seqidno:78、seqidno:79或seqidno:80。酶可通过添加剂颗粒(例如酶颗粒)或以包封物的形式掺入清洁组合物中，或者可以液体制剂的形式加入。优选地，酶通过包封物掺入清洁组合物中。包封酶促进了酶在组合物中的稳定性，并有助于抵消组合物中存在的任何不利化合物例如漂白剂、蛋白酶、表面活性剂、螯合剂等的影响。存在于添加剂颗粒中的氢过氧脂肪酸生成酶可以是添加剂颗粒中唯一的酶，或者可以与一种或多种附加的辅酶组合存在于添加剂颗粒中。必要时，该组合物包含，提供获取途径或原位形成对于酶的有效功能而言所必需的任何附加底物。例如，提供分子氧作为脂氧合酶、α-双加氧酶、二醇合酶和其他含有dox结构域的酶的附加底物。分子氧可以从大气中获得，或者从可被转化以原位生成氧的前体获得。在许多应用中，来自大气的氧可以足够的量存在。类似地，过氧化氢作为过加氧酶的附加底物提供，并且可添加到清洁组合物中或在清洁过程中原位生成，例如通过醇氧化酶对其对应底物的作用。此外，需要将水作为脂肪酸水合酶的附加底物，并且水可以是清洁组合物的一部分或在清洁过程中加入。表面活性剂本发明的消费产品组合物还包含表面活性剂，所述表面活性剂的含量按所述组合物的重量计优选为约0.01％至约60％，优选约5％至约50％，更优选约8％至约40％。在一些方面，所述消费产品组合物包含相对低含量的表面活性剂，因为污垢转化酶可将直接位于被清洁表面上的含脂肪酸的污垢转化为活性脂肪酸衍生物物质，以便有助于从表面清洁污垢。因此，此类活性脂肪酸衍生物物质可减少在消费产品组合物中掺入较大量的表面活性剂的需求。此外，较低含量的表面活性剂可有利于本文所用的污垢转化酶的稳定性和活性。因此，所述消费产品组合物优选包含按所述组合物的重量计约0.01％至约2％，优选约0.01％至约1.5％，优选约0.01％至约1％，优选约0.01％至约0.5％，优选约0.01％至约0.2％，优选约0.01％至约0.1％的表面活性剂。合适的表面活性剂选自阴离子表面活性剂、非离子表面活性剂、两性离子表面活性剂、两性表面活性剂、阳离子表面活性剂、以及它们的混合物；如果该组合物是衣物洗涤剂或洗发剂，则通常采用阴离子和非离子表面活性剂。如果组合物是织物柔软剂或毛发调理剂，则通常使用阳离子表面活性剂。适用于所述组合物中的阴离子表面活性剂包括烷基硫酸盐和烷基醚硫酸盐。其它合适的阴离子表面活性剂为有机硫酸反应产物的水溶性盐。其它合适的阴离子表面活性剂为用羟乙磺酸酯化并且用氢氧化钠中和的脂肪酸的反应产物。其它类似的阴离子表面活性剂描述于美国专利2,486,921；2,486,922；和2,396,278中。用于所述组合物中的示例性阴离子表面活性剂包括月桂基硫酸铵、月桂基聚氧乙烯醚硫酸铵、月桂基硫酸三乙基胺、月桂基聚氧乙烯醚硫酸三乙基胺、月桂基硫酸三乙醇胺、月桂基聚氧乙烯醚硫酸三乙醇胺、月桂基硫酸单乙醇胺、月桂基聚氧乙烯醚硫酸单乙醇胺、月桂基硫酸二乙醇胺、月桂基聚氧乙烯醚硫酸二乙醇胺、月桂酸单甘油酯硫酸钠、月桂基硫酸钠、月桂基聚氧乙烯醚硫酸钠、月桂基硫酸钾、月桂基聚氧乙烯醚硫酸钾、月桂基肌氨酸钠、月桂酰肌氨酸钠、月桂基肌氨酸、椰油酰肌氨酸、椰油酰硫酸铵、月桂酰硫酸铵、椰油酰硫酸钠、月桂酰硫酸钠、椰油酰硫酸钾、月桂基硫酸钾、月桂基硫酸三乙醇胺、月桂基硫酸三乙醇胺、椰油酰硫酸单乙醇胺、月桂基硫酸单乙醇胺、十三烷基苯磺酸钠、十二烷基苯磺酸钠、椰油酰羟乙磺酸钠以及它们的组合。在另一个实施方案中，阴离子表面活性剂为月桂基硫酸钠或月桂基聚氧乙烯醚硫酸钠。所述组合物可包含非离子表面活性剂。组合物可包含按所述组合物的重量计至多0.01％至约30％，或约0.01％至约20％，还或约0.1％至约10％的非离子表面活性剂。在一些示例中，非离子表面活性剂可包括乙氧基化非离子表面活性剂。适用于本文的为式r(oc2h4)noh的乙氧基化醇和乙氧基化烷基酚，其中r选自含有约8至约20个碳原子的脂族烃基团、和其中烷基基团含有约8至约12个碳原子的烷基苯基基团，并且n的平均值为约5至约15。合适的非离子表面活性剂为式r1(oc2h4)noh的那些，其中r1为c10-c16烷基基团或c8-c12烷基苯基基团，并且n为3至约80。在一个方面，尤其可用的材料为c9-c15醇与每摩尔醇约5至约20摩尔环氧乙烷的缩合产物。合适的阳离子表面活性剂包括可递送织物护理有益效果的那些，其非限制性实例包括：脂肪胺；季铵表面活性剂；和咪唑啉季铵盐材料。阳离子表面活性剂的非限制性示例为n,n-双(硬脂酰-氧乙基)n,n-二甲基氯化铵、n,n-双(牛油酰-氧乙基)n,n-二甲基氯化铵、n,n-双(硬脂酰-氧乙基)n-(2-羟乙基)n-甲基甲硫酸铵；1,2二(硬脂酰氧基)3三甲基丙基氯化铵；二亚烷基二甲基铵盐诸如二低芥酸菜籽基二甲基氯化铵、二(硬)牛油二甲基氯化铵、二低芥酸菜籽基二甲基甲酯硫酸铵；1-甲基-1-硬脂酰氨基乙基-2-硬脂酰基咪唑啉甲基硫酸盐；1-牛油基酰氨基乙基-2-牛油基咪唑啉；n,n″-二烷基二亚乙基三胺；用脂肪酸酯化的n-(2-羟乙基)-1,2-乙二胺或n-(2-羟基异丙基)-1,2-乙二胺与乙醇酸的反应产物，其中脂肪酸为(氢化)牛油脂肪酸、棕榈脂肪酸、氢化棕榈脂肪酸、油酸、油菜籽脂肪酸、氢化油菜籽脂肪酸；聚甘油酯(pge)、油性糖衍生物和蜡乳液以及上述物质的混合物。阳离子表面活性剂可用作消费产品组合物中的调理剂，诸如在织物软化组合物或毛发调理组合物中用作调理剂。适用于毛发护理组合物中的两性去污表面活性剂包括被广泛地描述为脂族仲胺和叔胺的衍生物的那些表面活性剂，其中脂族基团可为直链或支链，并且其中脂族取代基中的一个包含约8至约18个碳原子，并且一个脂族取代基包含阴离子基团诸如羧基、磺酸根、硫酸根、磷酸根或膦酸根。用于本发明的毛发护理组合物中的示例性两性去污表面活性剂包括椰油酰两性基乙酸盐、椰油酰两性基二乙酸盐、月桂酰两性基乙酸盐、月桂酰两性基二乙酸盐、以及它们的混合物。适用于毛发护理组合物中的两性离子去污表面活性剂包括被广泛地描述为脂族季铵、鏻鎓和锍鎓化合物的衍生物的那些表面活性剂，其中脂族基团可为直链或支链，并且其中脂族取代基中的一个包含约8至约18个碳原子，并且一个脂族取代基包含阴离子基团诸如羧基、磺酸根、硫酸根、磷酸根或膦酸根。在另一个实施方案中，选择两性离子化物，诸如甜菜碱。合适的两性离子表面活性剂或两性表面活性剂的非限制性示例描述于美国专利5,104,646和5,106,609中，这些文献全文以引用方式并入本文。其他适用于组合物中的阴离子表面活性剂、两性离子表面活性剂、两性表面活性剂或任选的附加表面活性剂的非限制性示例描述于mccutcheon的《emulsifiersanddetergents》(1989年鉴，由m.c.publishingco.)出版和美国专利3,929,678、2,658,072；2,438,091；2,528,378中，这些文献全文以引用方式并入本文。在一个方面，可优选的是消费产品组合物不含硫酸盐表面活性剂。在此类方面，表面活性剂优选为糖基表面活性剂或氨基酸基表面活性剂，例如选自癸基葡糖苷、羟乙基磺酸盐、谷氨酸盐以及它们的混合物的表面活性剂。在一个方面，所述消费产品组合物可包含阴离子表面活性剂和辅助表面活性剂，所述辅助表面活性剂选自两性表面活性剂、两性离子表面活性剂以及它们的混合物。优选地，两性表面活性剂是氧化胺表面活性剂，并且两性离子表面活性剂是甜菜碱表面活性剂。优选地，阴离子表面活性剂与一种或多种辅助表面活性剂的重量比小于9:1，更优选5:1至1:1，更优选4:1至2:1。优选地，主要辅助表面活性剂体系是两性表面活性剂。优选地，主要辅助表面活性剂体系是氧化胺表面活性剂，并且其中所述组合物包含重量比小于9:1，更优选5:1至1:1，更优选4:1至2:1，优选3:1至2.5:1的阴离子表面活性剂和氧化胺表面活性剂。优选地，对于本发明的组合物，其中表面活性剂体系包含一种或多种阴离子表面活性剂和一种或多种辅助表面活性剂，其中阴离子表面活性剂为烷基硫酸盐和烷基烷氧基硫酸盐的混合物，辅助表面活性剂为烷基二甲基氧化胺，并且其中阴离子表面活性剂与辅助表面活性剂的重量比为4:1至2:1。优选的氧化胺是烷基二甲基氧化胺或烷基酰氨基丙基二甲基氧化胺，更优选地烷基二甲基氧化胺，并且尤其是椰油二甲基氧化胺。氧化胺可具有直链或支链的烷基部分。所述组合物还可包含c10ao，尤其是正癸基二甲胺，并且优选包含按总氧化胺的重量计小于5％，优选小于3％的c8氧化胺，例如c8二甲基氧化胺。辅助表面活性剂可为两性离子表面活性剂。两性离子表面活性剂的合适实例包括甜菜碱，优选烷基甜菜碱、烷基酰胺基甜菜碱、以及它们的混合物。最优选椰油酰胺丙基甜菜碱。所述消费产品组合物还可包含辅助表面活性剂，所述辅助表面活性剂包括非离子表面活性剂。合适的非离子表面活性剂包括脂族醇与1摩尔至25摩尔环氧乙烷的缩合产物。脂族醇的烷基链可为直链或支链、伯烷基或仲烷基，并通常含有8至22个碳原子。尤其优选的是具有具有10至18个碳原子，优选地10至15个碳原子的烷基的醇与每摩尔醇2摩尔至18摩尔，优选地2摩尔至15摩尔，更优选5摩尔至12摩尔的环氧乙烷的缩合产物。高度优选的非离子表面活性剂是格尔伯特醇与每摩尔醇2摩尔至18摩尔，优选地2摩尔至15摩尔，更优选地5摩尔至12摩尔的环氧乙烷的缩合产物。优选地，非离子表面活性剂是烷基乙氧基化表面活性剂，优选在其烷基链中包含9至15个碳原子并且每摩尔醇包含5至12个环氧乙烷单元。用于本文的其他合适的非离子表面活性剂包括脂肪醇聚乙二醇醚、烷基多聚葡糖苷和脂肪酸葡糖酰胺，优选烷基多聚葡糖苷。优选地，烷基多聚葡糖苷表面活性剂是c8-c16烷基多聚葡糖苷表面活性剂，优选c8-c14烷基多聚葡糖苷表面活性剂，优选具有在0.1至3之间，更优选在0.5至2.5之间，甚至更优选在1至2之间的平均聚合度。最优选地，烷基多聚葡糖苷表面活性剂具有在10至16，优选在10至14之间，最优选在12至14之间的平均烷基碳链长度，其中平均聚合度为在0.5至2.5之间，优选在1至2之间，最优选在1.2至1.6之间。c8-c16烷基多聚葡糖苷可从几个供应商处商购获得(例如，购自seppic公司的表面活性剂；和购自basf公司的600csup、650ec、600csup/mb和650ec/mb)。优选地，组合物包含2:1至50:1，优选2:1至10:1的比率的阴离子表面活性剂和非离子表面活性剂。消费产品助剂成分本文的消费产品组合物可任选地包含许多其他消费产品助剂成分，诸如酶稳定剂、辅酶、盐、水溶助长剂、螯合剂、助洗剂、分散剂、染料转移抑制剂、漂白剂、稳定剂/增稠剂、香料、调理剂、调色剂、结构剂、溶剂、含水载体、以及它们的混合物。消费产品助剂成分还包括洗擦颗粒、恶臭控制剂、颜料、染料、遮光剂、ph调节剂和缓冲物(例如，羧酸如柠檬酸、hcl、naoh、koh、链烷醇胺、磷酸和磺酸，碳酸盐如碳酸钠、碳酸氢盐、倍半碳酸盐、硼酸盐、硅酸盐、磷酸盐、咪唑等)。酶稳定剂优选地，本发明的组合物还包含酶稳定剂，所述酶稳定剂选自化学稳定剂和物理稳定剂，优选地，所述物理稳定剂包括包封所述酶。合适的酶稳定剂可选自由下列构成的组：(a)优选选自由下列构成的组的单价、二价和/或三价阳离子：碱土金属、碱金属、铝、铁、铜和锌的无机或有机盐，优选碱金属和碱土金属，优选碱金属和碱土金属盐与卤化物、硫酸盐、亚硫酸盐、碳酸盐、碳酸氢盐、硝酸盐、亚硝酸盐、磷酸盐、甲酸盐、乙酸盐、丙酸盐、柠檬酸盐、马来酸盐、酒石酸盐、琥珀酸盐、草酸盐、乳酸盐、以及它们的混合物。在优选的实施方案中，盐选自由下列构成的组：氯化钠、氯化钙、氯化钾、硫酸钠、硫酸钾、乙酸钠、乙酸钾、甲酸钠、甲酸钾、乳酸钙、硝酸钙以及它们的混合物。最优选的是选自由下列构成的组的盐：氯化钙、氯化钾、硫酸钾、乙酸钠、乙酸钾、甲酸钠、甲酸钾、乳酸钙、硝酸钙以及它们的混合物的盐，特别是选自由下列构成的组的钾盐：氯化钾、硫酸钾、乙酸钾、甲酸钾、丙酸钾、乳酸钾以及它们的混合物。最优选的是乙酸钾和氯化钾。优选的钙盐是甲酸钙、乳酸钙和硝酸钙，包括硝酸钙四水合物。可优选甲酸钙和甲酸钠盐。这些阳离子以按组合物的总重量计至少0.01重量％，优选至少0.03重量％，更优选至少0.05重量％，最优选至少0.25重量％至最多2重量％或甚至最多1重量％的量存在。这些盐被配制为相对于组合物总重量的0.1重量％至5重量％，优选0.2重量％至4重量％，更优选0.3重量％至3重量％，最优选0.5重量％至2重量％。其它酶稳定剂可选自由下列构成的组：(b)选自由低聚糖、多糖以及它们的混合物构成的组的碳水化合物，如wo201219844中所述的单糖甘油酸酯；(c)高效可逆蛋白酶抑制剂，高效可逆蛋白酶抑制剂选自由下列构成的组：苯基硼酸以及它们的衍生物，优选4-甲酰基苯基硼酸；(d)醇类，如1,2-丙烷二醇、丙二醇；(e)肽醛稳定剂，如三肽醛，如cbz-gly-ala-tyr-h，或二取代的丙氨酰胺；(f)羧酸，如wo2012/19849中所述的苯基烷基二羧酸或如wo2012/19848中所述的在苄基的至少两个碳原子上包含羧基的多取代苄基羧酸，邻苯二甲酰谷氨酰胺酸，邻苯二甲酰基天冬酰胺酸，氨基邻苯二甲酸和/或低聚氨基-联苯-低聚羧酸；以及(g)它们的混合物。辅酶本发明优选的组合物包含选自淀粉酶、脂肪酶、蛋白酶、纤维素酶、脂氧合酶、二醇合酶以及它们的混合物的一种或多种附加的辅酶。本发明的甚至更优选的组合物包含选自脂肪酶、蛋白酶、纤维素酶、淀粉酶以及它们的任意组合的一种或多种辅酶。本发明的最优选的组合物包含选自脂肪酶、蛋白酶、淀粉酶以及它们的任意组合的一种或多种辅酶。特别优选的是，本发明的组合物另外包含蛋白酶辅酶。由于油酸和其他泡沫抑制不饱和脂肪酸存在于体垢甚至人体皮肤中，因为蛋白酶用作皮肤护理剂或分解蛋白质污垢，因此释放的脂肪酸被分解，防止泡沫抑制。特别优选的是，本发明的组合物另外包含淀粉酶辅酶。由于油性污垢通常被捕获在淀粉质污垢中，所以淀粉酶和不饱和脂肪酸转化酶一起协同作用：通过用淀粉酶分解淀粉质污垢来释放脂肪酸污垢，因此，不饱和脂肪酸转化酶特别有效地确保不会对洗涤液体中的泡沫产生负面影响。特别优选的是，本发明的组合物另外包含脂肪酶辅酶。脂肪酶将脂肪酯污垢分解成脂肪酸，然后氢过氧脂肪酸生成酶、过加氧酶和脂肪酸水合酶作用于脂肪酸，生成泡沫或清洁促进剂。在本发明的另一个实施方案中，消费产品组合物包含一种或多种辅酶，所述一种或多种辅酶选自：非特异性单加氧酶(ec1.14.14.1)、烷烃1-单加氧酶(ec1.14.15.3)、油酸12-羟化酶(ec1.14.18.4)、亚油酸异构酶(ec5.2.1.5)、亚油酸(10e,12z)-异构酶(ec5.3.3.b2)、脂肪酸脱羧酶(olet样)、铁依赖性脱羧酶(unda样)、其他cyp450单加氧酶、以及它们的混合物。当存在时，辅酶通常以按组合物的重量计(基于活性蛋白质)约0.0001％至约1％，优选约0.0005％至约0.5％，更优选约0.005％至约0.1％的量存在。盐本发明的组合物可任选地包含按总组合物的重量计0.01％至3％，优选0.05％至2％，更优选0.2％至1.5％，或最优选0.5％至1％的盐，优选一价、二价无机盐或它们的混合物，优选氯化钠。最优选地，所述组合物可选地或进一步包含按组合物的重量计0.01％至2％，优选0.1％至1％，更优选0.2％至0.8％的量的多价金属阳离子，优选地所述多价金属阳离子是镁、铝、铜、钙或铁，更优选地是镁，最优选地所述多价盐是氯化镁。据信，多价阳离子的使用有助于在油水和气水界面处形成增强泡沫的蛋白质/蛋白质、表面活性剂/表面活性剂或混合蛋白质/表面活性剂网络。水溶助长剂本发明的组合物可以任选地包含按总组合物的重量计1％至10％，或优选0.5％至10％，更优选1％至6％，或最优选0.1％至3％，或它们的组合的水溶助长剂，优选异丙基苯磺酸钠。适用于本文的其他水溶助长剂包括阴离子型水溶助长剂，尤其是二甲苯磺酸钠、二甲苯磺酸钾和二甲苯磺酸铵，甲苯磺酸钠、甲苯磺酸钾和甲苯磺酸铵，异丙基苯磺酸钠、异丙基苯磺酸钾和异丙基苯磺酸铵、以及它们的混合物，如美国专利3,915,903中所公开的。螯合剂本文洗涤剂组合物可包含按总组合物的重量计0.1％至20％，优选0.2％至5％，更优选0.2％至3％含量的螯合剂。合适的螯合剂包括柠檬酸盐；含氮的无磷氨基羧酸盐，诸如edds、edta和dtpa；氨基膦酸盐，诸如二亚乙基三胺五亚甲基膦酸和乙二胺四亚甲基膦酸；无氮膦酸盐，例如hedp；以及含氮或氧的无磷无羧酸根的螯合剂，诸如具有某些大环n-配体的一般类别化合物，诸如已知用于漂白催化剂体系中的那些。用于本发明组合物的其他合适的螯合剂包括羧酸盐螯合剂、氨基羧酸盐螯合剂、氨基膦酸盐螯合剂、以及它们的混合物。优选地，螯合剂选自mgda(甲基甘氨酸-n,n-二乙酸)、glda(谷氨酸-n,n-二乙酸)、以及它们的混合物。助洗剂所述组合物还可包含按所述组合物的重量计约0.1％至80％的助洗剂。液体形式的组合物一般包含按所述组合物的重量计约1％至10％的助洗剂组分。颗粒形式的组合物一般包含按所述组合物的重量计约1％至50％的助洗剂组分。洗涤剂助剂是本领域熟知的，并且可包括例如磷酸盐以及各种有机和无机无磷助洗剂。可用于本文的水溶性无磷有机助洗剂包括聚乙酸、羧酸、聚羧酸和多羟基磺酸的各种碱金属盐、铵盐和取代的铵盐。聚乙酸盐助洗剂和聚羧酸盐助洗剂的示例为乙二胺四乙酸、次氮基三乙酸、氧联二琥珀酸、苯六甲酸、多羧酸苯和柠檬酸的钠、钾、锂、铵和取代的铵盐。其它聚羧酸盐助洗剂为氧联二琥珀酸盐和醚羧酸盐助洗剂组合物，该醚羧酸盐助洗剂组合物包含酒石酸一琥珀酸盐和酒石酸二琥珀酸盐的组合。用于液体洗涤剂中的助洗剂包括柠檬酸。合适的无磷无机助洗剂包括硅酸盐、硅铝酸盐、硼酸盐和碳酸盐，诸如碳酸钠和碳酸钾、碳酸氢钠和碳酸氢钾、倍半碳酸钠和倍半碳酸钾、十水四硼酸钠和十水四硼酸钾、以及具有约0.5至约4.0、或约1.0至约2.4的sio2与碱金属氧化物的重量比的硅酸钠和硅酸钾。也可用的是硅铝酸盐，包括沸石。分散剂所述组合物可包含按所述组合物的重量计约0.1％至约10％的分散剂。合适的水溶性有机材料是均聚酸或共聚酸或它们的盐，其中多元羧酸可含有至少两个彼此分离不超过两个碳原子的羧基基团。分散剂也可为聚胺的烷氧基化衍生物和/或季铵化衍生物。染料转移抑制剂所述组合物还可包含按所述组合物的重量计约0.0001％，约0.01％，约0.05％，至按所述组合物的重量计约10％，约2％，或甚至约1％的一种或多种染料转移抑制剂，如聚乙烯吡咯烷酮聚合物、聚胺n-氧化物聚合物、n-乙烯基吡咯烷酮和n-乙烯基咪唑的共聚物、聚乙烯噁唑烷酮和聚乙烯咪唑或它们的混合物。漂白剂体系适用于本文的漂白剂体系包含一种或多种漂白剂。合适的漂白剂的非限制性示例包括催化金属配合物；活性过氧源；漂白活化剂；漂白增效剂；光漂白剂；漂白酶；自由基引发剂；h2o2；次氯酸盐漂白剂；过氧源，包括过硼酸盐和/或过碳酸盐以及它们的组合。合适的漂白活化剂包括可全水解的酯和可全水解的酰亚胺，诸如四乙酰基乙二胺、辛酰基己内酰胺、苯甲酰氧基苯磺酸酯、壬酰氧基苯磺酸酯、苯甲酰基戊内酰胺、十二烷酰氧基苯磺酸酯。其它漂白剂包括过渡金属与具有指定稳定常数的配体的金属配合物。稳定剂/增稠剂所述组合物可包含一种或多种稳定剂和增稠剂。可使用任何合适含量的稳定剂；示例性含量包括按组合物的重量计约0.01％至约20％、约0.1％至约10％、或约0.1％至约3％。适用于本文的稳定剂的非限制性示例包括结晶的含羟基稳定剂、三羟基硬脂酸甘油酯、氢化油、或它们的变型、以及它们的组合。在一些方面，结晶的含羟基稳定剂可为水不溶性蜡状物质，包括脂肪酸、脂肪酸酯或脂肪皂。在其它方面，结晶的含羟基稳定剂可为蓖麻油的衍生物，诸如氢化蓖麻油衍生物，例如蓖麻蜡。含羟基稳定剂公开于美国专利6,855,680和7,294,611中。其它稳定剂包括增稠稳定剂，诸如树胶和其它类似多糖，例如结冷胶、角叉菜胶和其它已知类型的增稠剂和流变添加剂。该类示例性稳定剂包括树胶型聚合物(例如黄原胶)、聚乙烯醇及其衍生物、纤维素及其衍生物(包括纤维素醚和纤维素酯)、和罗望子胶(例如包括木葡聚糖聚合物)、瓜尔胶、刺槐豆胶(在一些方面，包括半乳甘露聚糖聚合物)、以及其它工业树胶和聚合物。调理剂消费产品组合物可包含一种或多种调理剂。调理剂包括用于向表面例如毛发或织物提供特定调理或软化有益效果的材料。可用于本发明的消费产品组合物中的调理剂通常包括水不溶性、水分散性、非挥发性、可形成乳化的液体颗粒的液体。合适的调理剂为特征通常为有机硅、有机调理油或它们的组合的那些调理剂，或者在含水表面活性剂基质中以其他方式形成液体、分散颗粒的那些调理剂。阳离子表面活性剂，例如上文所述的季铵化合物，也可以用作本发明组合物中的调理剂。一种或多种调理剂以按所述组合物的重量计约0.01％至约10％，约0.1％至约8％，和约0.2％至约4％存在。有机硅合适的有机硅包含si-o部分，并且可选自(a)非官能化硅氧烷聚合物，(b)官能化硅氧烷聚合物，以及它们的组合。有机硅的分子量通常通过参考材料的粘度而示出。在一个方面，有机硅在25℃下可具有约10厘沲至约2,000,000厘沲的粘度。在另一方面，合适的有机硅在25℃下可具有约10厘沲至约800,000厘沲的粘度。合适的有机硅可为直链、支链或交联的。在一些示例中，有机硅可包括环状有机硅。环状有机硅可包括式[(ch3)2sio]n的环甲基硅酮，其中n为可在约3至约7、或约5至约6范围内的整数。在一些示例中，有机硅可包括官能化硅氧烷聚合物。官能化硅氧烷聚合物可包含一个或多个官能团部分，该官能团部分选自氨基、酰氨基、烷氧基、羟基、聚醚、羧基、氢离子、巯基、硫酸根、磷酸根、和/或季铵部分。这些部分可通过二价亚烷基基团直接连接到硅氧烷主链(即“侧基”)，或可为主链的一部分。合适的官能化硅氧烷聚合物包括选自以下的材料：氨基有机硅、酰氨基有机硅、有机硅聚醚、有机硅-氨基甲酸酯聚合物、季abn有机硅、氨基abn有机硅、以及它们的组合。在一些示例中，官能化硅氧烷聚合物可包括有机硅聚醚，其还称为“聚二甲基硅氧烷共聚多元醇”。一般来讲，有机硅聚醚包含聚二甲基硅氧烷主链与一个或多个聚氧化亚烷基链。聚氧化亚烷基部分可作为侧链或作为末端嵌段掺入聚合物中。在一些示例中，官能化硅氧烷聚合物可包括氨基有机硅。在一些示例中，有机硅可包括胺abn有机硅和季abn有机硅。此类有机硅一般通过二胺与环氧化物反应来制备。在一些示例中，官能化硅氧烷聚合物可包括有机硅-氨基甲酸酯。这些可以商品名从wackersilicones商购获得。有机硅材料通常用作消费产品组合物中的调理剂，诸如在织物软化组合物或毛发调理组合物中用作调理剂。有机调理材料本发明的组合物的调理剂还可包含单独或与其它调理剂诸如上述有机硅组合的至少一种有机调理材料诸如油或蜡。有机材料可以是非聚合的、低聚的或聚合的。它可为油或蜡的形式，并且可以纯制剂或以预乳化形式添加。有机调理材料的一些非限制性示例包括但不限于：i)烃油；ii)聚烯烃；iii)脂肪酯；iv)氟化调理化合物；v)脂肪醇；vi)烷基葡糖苷和烷基葡糖苷衍生物；vii)季铵化合物；viii)具有至多约2,000,000的分子量的聚乙二醇和聚丙二醇，包括具有ctfa名称peg-20200、peg-400、peg-600、peg-1000、peg-2m、peg-7m、peg-14m、peg-45m的那些、以及它们的混合物。香料消费产品助剂成分可包含香料，该香料为除了微胶囊之外另外添加到消费产品组合物中的纯香料。因此，消费产品组合物可包含纯香料和包含香料作为微胶囊的芯材料的微胶囊。纯香料和微胶囊的香料可相同或可不同。调色剂组合物可包含调色剂(有时被称为遮光剂、上蓝剂或美白剂)。调色剂通常向被处理表面例如织物提供蓝色或紫色色调。调色剂能够单独使用或组合使用，以产生特定的调色色调和/或对不同的表面类型调色。这可例如通过将红色和蓝绿色染料混合以产生蓝色或紫色色调来提供。调色剂可选自任何已知化学类别的染料，包括但不限于吖啶、蒽醌类(包括多环醌类)、吖嗪、偶氮(例如，单偶氮、双偶氮、三偶氮、四偶氮、多偶氮)、包括预金属化偶氮、苯并二呋喃和苯并二呋喃酮、类胡萝卜素、香豆素、花菁、二氮杂半花菁、二苯甲烷、甲臜、半花菁、靛蓝类、甲烷、萘酰亚胺、萘醌、硝基和亚硝基、噁嗪、酞菁、吡唑类、二苯乙烯、苯乙烯基、三芳基甲烷、三苯甲烷、氧杂蒽以及它们的混合物。合适的织物调色剂还包括染料、染料-粘土缀合物、以及有机颜料和无机颜料。合适的染料包括小分子染料和聚合物染料。合适的小分子染料包括选自以下的小分子染料：属于酸性、直接、碱性、活性、或水解的活性、溶剂或分散染料的颜色索引(c.i.)分类的染料，例如被分类为蓝、紫、红、绿或黑的染料，并且单独地或组合地提供期望的色调。在另一方面，合适的小分子染料包括选自色素索引(societyofdyersandcolourists，bradford，uk)的下列编号的小分子染料：直接紫染料诸如9、35、48、51、66和99，直接蓝染料诸如1、71、80和279，酸性红染料诸如17、73、52、88和150，酸性紫染料诸如15、17、24、43、49和50，酸性蓝染料诸如15、17、25、29、40、45、75、80、83、90和113，酸性黑染料诸如1，碱性紫染料诸如1、3、4、10和35，碱性蓝染料诸如3、16、22、47、66、75和159，分散染料或溶剂染料(us8,268,016b2)，或如us7,208,459b2中所公开的染料，以及它们的混合物。在另一方面，合适的小分子染料包括选自以下的小分子染料：c.i.编号酸性紫17、酸性蓝80、酸性紫50、直接蓝71、直接紫51、直接蓝1、酸性红88、酸性红150、酸性蓝29、酸性蓝113或它们的混合物。合适的聚合物染料包括选自以下的聚合物染料：含有共价结合(有时被称为缀合)的色原体的聚合物(染料-聚合物缀合物)(例如具有共聚至该聚合物主链中的色原体的聚合物)以及它们的混合物。聚合物染料包括描述于us7,686,892b2中的那些。在一些示例中，适宜的聚合物染料包括选自下列物质的聚合物染料：以商品名(milliken，spartanburg，southcarolina，usa)销售的织物-实体着色剂、由至少一种活性染料形成的染料-聚合物缀合物、以及选自包含以下部分的聚合物的聚合物，所述部分选自羟基部分、伯胺部分、仲胺部分、硫醇部分、以及它们的混合物。在一些示例中，适宜的聚合物染料包括选自下列的聚合物染料：紫ct，与活性蓝、活性紫或活性红染料共价结合的羧甲基纤维素(cmc)，如与c.i.活性蓝19缀合的cmc(由megazyme,wicklow,ireland以产品名azo-cm-cellulose，产品代码s-acmc销售)、烷氧基化的三苯基-甲烷聚合物着色剂、烷氧基化的噻吩聚合物着色剂、以及它们的混合物。合适的染料粘土缀合物包括选自以下的染料粘土缀合物：至少一种阳离子/碱性染料和绿土粘土、以及它们的混合物。在另一方面，合适的染料粘土缀合物包括选自以下的染料粘土缀合物：一种阳离子/碱性染料，其选自：c.i.碱性黄1至108，c.i.碱性橙1至69，c.i.碱性红1至118，c.i.碱性紫1至51，c.i.碱性蓝1至164，c.i.碱性绿1至14，c.i.碱性棕1至23、ci碱性黑1至11以及粘土，该粘土选自蒙脱石粘土、锂蒙脱石粘土、皂石粘土以及它们的混合物。在另一个方面，合适的染料粘土缀合物包括选自以下的染料粘土缀合物：蒙脱石碱性蓝b7c.i.42595缀合物、蒙脱石碱性蓝b9c.i.52015缀合物、蒙脱石碱性紫v3c.i.42555缀合物、蒙脱石碱性绿g1c.i.42040缀合物、蒙脱石碱性红r1c.i.45160缀合物、蒙脱石c.i.碱性黑2缀合物、锂蒙脱石碱性蓝b7c.i.42595缀合物、锂蒙脱石碱性蓝b9c.i.52015缀合物、锂蒙脱石碱性紫v3c.i.42555缀合物、锂蒙脱石碱性绿g1c.i.42040缀合物、锂蒙脱石碱性红r1c.i.45160缀合物、锂蒙脱石c.i.碱性黑2缀合物、皂碱性蓝b7c.i.42595缀合物、皂碱性蓝b9c.i.52015缀合物、皂碱性紫v3c.i.42555缀合物、皂碱性绿g1c.i.42040缀合物、皂碱性红r1c.i.45160缀合物、皂c.i.碱性黑2缀合物以及它们的混合物。合适的颜料包括选自以下的颜料：黄烷士酮、靛蒽醌、含有1至4个氯原子的氯化靛蒽醌、皮蒽酮、二氯皮蒽酮、单溴二氯皮蒽酮、二溴二氯皮蒽酮、四溴皮蒽酮、二萘嵌苯-3,4,9,10-四羧酸二酰亚胺，其中酰亚胺基团可为未取代的或者被c1-c3-烷基或苯基或杂环基团取代，并且其中苯基和杂环基团可另外带有不提供水中溶解度的取代基、蒽素嘧啶羧酸酰胺、蒽酮紫、异蒽酮紫、二噁嗪颜料、每个分子可含有至多2个氯原子的铜酞菁、多氯铜酞菁或每个分子含有至多14个溴原子的多溴氯铜酞菁、以及它们的混合物。在另一方面，合适的颜料包括选自以下的颜料：群青蓝(c.i.颜料蓝29)、群青紫(c.i.颜料紫15)、单星蓝以及它们的混合物。结构剂可加入以充分悬浮包含有益剂的递送颗粒的有用结构剂材料包括多糖，例如结冷胶、蜡质玉米或马齿种玉米淀粉、辛烯基琥珀酸化淀粉、衍生的淀粉诸如羟乙基化或羟丙基化淀粉、角叉菜胶、瓜耳胶、果胶、黄原胶，以及它们的混合物；改性纤维素，诸如水解纤维素乙酸酯、羟丙基纤维素、甲基纤维素、以及它们的混合物；改性蛋白质，诸如明胶；氢化和未氢化的聚烯烃、以及它们的混合物；无机盐，例如氯化镁、氯化钙、甲酸钙、甲酸镁、氯化铝、高锰酸钾、合成锂皂石粘土、膨润土以及它们的混合物；与无机盐组合的多糖；季铵化聚合物材料，例如聚醚胺、烷基三甲基氯化铵、二酯二牛油基氯化铵；咪唑；具有小于6.0的pka的非离子聚合物，例如聚乙烯亚胺、聚乙烯亚胺乙氧基化物；聚氨酯。此类材料可得自cpkelcocorp.(sandiego,california,usa)；degussaag或dusseldorf,germany；basfag(ludwigshafen,germany)；rhodiacorp.(cranbury,newjersey,usa)；bakerhughescorp.(houston,texas,usa)；herculescorp.(wilmington,delaware,usa)；agriuminc.(calgary,alberta,canada)、isp(newjersey,u.s.a.)。溶剂本发明的组合物可任选包含有机溶剂。合适的有机溶剂包括c4-14醚和二醚、多元醇、二醇、烷氧基化二醇、c6-c16二醇醚、烷氧基化芳族醇、芳族醇、脂族直链或支链醇、烷氧基化脂族直链或支链醇、烷氧基化c1-c5醇、c8-c14烷基和环烷基烃以及卤代烃、以及它们的混合物。优选地，有机溶剂包括醇、二醇和二醇醚，或者醇和二醇。所述组合物包含按所述总组合物的重量计0％至小于50％，优选0.01％至25％，更优选0.1％至10％，或最优选0.5％至5％的有机溶剂，优选醇，更优选乙醇、聚亚烷基二醇，更优选聚丙二醇、以及它们的混合物。含水载体本文的组合物可为可倾倒的液体形式(在环境条件下)。因此，此类组合物通常包含载体，其以约20重量％至约95重量％、或甚至约60重量％至约85重量％的含量存在。所述载体可包括水，或水和有机溶剂的可混溶混合物，并且在一个方面，可包括水与最小或不显著浓度的有机溶剂，除了另外作为其它组分的微量成分附带地掺入组合物中的那些。可用于本发明组合物实施方案中的载体包括水以及低级烷基醇和多元醇的水溶液。可用于本文的低级烷基醇是具有1至6个碳的一元醇，在一个方面，为乙醇和异丙醇。可用于本文的示例性多元醇包括丙二醇、己二醇、甘油和丙烷二醇。清洁表面的方法本发明涉及一种清洁上面存在包含脂肪酸的污垢的表面的方法，所述方法包括以下步骤：(a)使存在于所述表面上的所述污垢与消费产品组合物接触，所述消费产品组合物包含表面活性剂和选自以下的污垢转化酶：(i)α-双加氧酶；(ii)氢过氧脂肪酸生成酶，所述氢过氧脂肪酸生成酶选自脂氧合酶；和氢过氧脂肪酸转化酶，所述氢过氧脂肪酸转化酶选自环加氧酶(ec1.14.99.1)、丙二烯氧化物合酶(ec4.2.1.92)、氢过氧化物异构酶(ec4.2.1.92、ec5.3.99.1、ec5.4.4.5、ec5.4.4.6)、氢过氧化物裂解酶(ec4.2.1.92)、氢过氧化物脱水酶(ec4.2.1.92)、二乙烯基醚合酶(ec4.2.1.121、ec4.2.1.b8、ec4.2.1.b9)、9,12-十八碳二烯酸8-氢过氧化物8r-异构酶(ec5.4.4.5)、9,12-十八碳二烯酸8-氢过氧化物8s-异构酶(ec5.4.4.6)、7,10-氢过氧化物二醇合酶、环氧醇合酶、以及它们的混合物，其中所述氢过氧脂肪酸转化酶能够转化所述氢过氧脂肪酸生成酶的反应产物；(iii)多结构域酶，所述多结构域酶包含氢过氧脂肪酸结构域，所述氢过氧脂肪酸结构域包括双加氧酶结构域和氢过氧脂肪酸转化结构域，所述氢过氧脂肪酸转化结构域选自丙二烯氧化物合酶结构域、环氧醇合酶结构域、氢过氧化物裂解酶结构域和氢过氧化物异构酶结构域；(iv)羟基脂肪酸生成酶，所述羟基脂肪酸生成酶选自过加氧酶、脂肪酸水合酶、以及它们的混合物；以及(iv)它们的混合物；和(b)将所述表面上的所述污垢的所述脂肪酸转化为选自氢过氧脂肪酸、氢过氧脂肪酸衍生物、羟基脂肪酸以及它们的混合物的活性脂肪酸衍生物物质。本发明的方法可在表面上原位形成活性脂肪酸衍生物物质，从而在使用期间从消费产品组合物产生附加的清洁作用。这可提供增强的清洁有益效果和/或允许在消费产品组合物中使用降低的表面活性剂含量。所述方法还可包括从所述表面除去所述消费产品组合物的步骤，例如通过从所述表面冲洗所述组合物(例如用水)或从所述表面机械地除去所述组合物(例如通过从所述表面擦拭组合物)。所述方法还可包括用水稀释所述消费产品组合物以形成稀释的消费产品组合物，然后使所述表面与稀释的消费产品组合物接触的步骤。用本发明的消费产品组合物处理的优选表面包括选自毛发、皮肤、织物、盘碟、餐具和家用硬质表面的表面。在一个方面，污垢的脂肪酸选自单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、饱和脂肪酸、以及它们的混合物。优选地，污垢的脂肪酸选自肉豆蔻油酸、棕榈油酸、十六碳烯酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、鳕油酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物。优选地，污垢的脂肪酸选自棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物。优选地，污垢的脂肪酸是油酸。在一个方面，通过所述方法形成的活性脂肪酸衍生物物质是选自以下的氢过氧脂肪酸：2-氢过氧脂肪酸、8r-氢过氧脂肪酸、8s-氢过氧脂肪酸、9r-氢过氧脂肪酸、9s-氢过氧脂肪酸、10r-氢过氧脂肪酸、11r-氢过氧脂肪酸、11s-氢过氧脂肪酸、12r-氢过氧脂肪酸、12s-氢过氧脂肪酸、13r-氢过氧脂肪酸、13s-氢过氧脂肪酸、14r-氢过氧脂肪酸、14s-氢过氧脂肪酸、15s-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；优选地选自2r-氢过氧脂肪酸、不饱和8r-氢过氧脂肪酸、不饱和9r-氢过氧脂肪酸、不饱和11r-氢过氧脂肪酸、不饱和12r-氢过氧脂肪酸、不饱和12s-氢过氧脂肪酸、不饱和13s-氢过氧脂肪酸、不饱和15s-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；并且更优选2r-氢过氧脂肪酸。在一个方面，通过所述方法形成的活性脂肪酸衍生物物质是选自以下的氢过氧脂肪酸衍生物：二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇、以及它们的混合物；优选二羟基脂肪酸。在一个方面，通过所述方法形成的活性脂肪酸衍生物物质是选自以下的羟基脂肪酸：2-羟基脂肪酸、3-羟基脂肪酸、4-羟基脂肪酸、5-羟基脂肪酸、6-羟基脂肪酸、以及它们的混合物；优选2-羟基脂肪酸、3-羟基脂肪酸、以及它们的混合物。本发明还涉及一种清洁表面的方法，包括手动洗涤脏污制品，优选盘碟的方法，包括以下步骤：将本发明的组合物递送至一定体积的水中以形成洗涤溶液，以及将所述脏污制品浸入所述洗涤溶液中，其中所述脏污制品上的污垢包含至少一种选自以下的不饱和脂肪酸：单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、以及它们的混合物。优选的不饱和脂肪酸包括：肉豆蔻油酸、棕榈油酸、十六碳烯酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、鳕油酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物，更优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物，更优选油酸。优选地，由脂肪酸与氢过氧脂肪酸生成酶的转化反应所形成的所得氢过氧脂肪酸选自8r-羟基过氧脂肪酸、8s-羟基过氧脂肪酸、9r-氢过氧脂肪酸、9s-氢过氧脂肪酸、10r-氢过氧脂肪酸、11r-氢过氧脂肪酸、11s-氢过氧脂肪酸、12r-氢过氧脂肪酸、12s-氢过氧脂肪酸、13r-氢过氧脂肪酸、13s-氢过氧脂肪酸、14r-氢过氧脂肪酸、14s-氢过氧脂肪酸、15s-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；优选不饱和的8r-氢过氧脂肪酸、不饱和的8s-氢过氧脂肪酸、不饱和的9r-氢过氧脂肪酸、不饱和的10r-氢过氧脂肪酸、它们的衍生物、和它们的混合物。由氢过氧脂肪酸转化酶转化氢过氧脂肪酸而形成的氢过氧脂肪酸衍生物优选选自单羟基脂肪酸、二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇、以及它们的混合物，优选二羟基脂肪酸。优选地，在洗涤过程中，在含水洗涤液体中，氢过氧脂肪酸生成酶和氢过氧脂肪酸转化酶各自以0.005ppm至15ppm，优选0.01ppm至5ppm，更优选0.02ppm至0.5ppm的浓度存在。因此，本文的组合物将以其稀释形式施用到盘碟上。将脏污表面例如盘碟与有效量的，通常0.5ml至20ml(每25个待处理的盘碟)，优选3ml至10ml的稀释在水中的本发明洗涤剂组合物(优选液体形式)接触。洗涤剂组合物的实际用量将基于使用者的判断，并且将典型地根据以下因素：如，具体的组合物产品制剂，包括组合物中活性成分的浓度、待清洁的脏盘碟数量、盘碟脏的程度等。通常，将0.01ml至150ml，优选3ml至40ml的本发明的液体洗涤剂组合物与2,000ml至20,000ml，更典型地5,000ml至15,000ml的水在具有1,000ml至20,000ml，更典型地5,000ml至15,000ml范围内的体积容量的池中混合。将脏污盘碟浸入包含稀释组合物的池，然后用布、海绵或类似制品接触盘碟的脏污表面来实现清洁它们。可将布、海绵或类似制品在与盘碟表面接触之前浸入洗涤剂组合物和水的混合物中，然后典型地与盘碟表面接触1秒至10秒的时间，虽然实际时间将随每个应用和使用者的不同而不同。织物、海绵或类似制品与表面的接触优选伴随着表面的同时洗擦。另选地，本发明的组合物可直接递送到盘碟上，或通过在在水的存在下用组合物清洁盘碟之前，使包含组合物的清洁工具(如海绵)与盘碟接触，以及任选地冲洗。此类直接应用的盘碟洗涤方法对于清洁油脂盘碟特别有益，尤其是在油脂已被烘烤的情况下。本发明包括一种或多种氢过氧脂肪酸生成酶和一种或多种氢过氧脂肪酸转化酶在包含污垢的含水洗涤液体中提供增加的泡沫持久性的用途，其中所述污垢包含脂肪酸。酶优选包含在用于手动洗涤盘碟的洗涤剂组合物中，特别是本发明的洗涤剂组合物中。本发明还涉及本文所述的消费产品组合物将存在于表面上的污垢的脂肪酸转化为活性脂肪酸衍生物物质的用途，所述活性脂肪酸衍生物物质选自氢过氧脂肪酸、氢过氧脂肪酸衍生物、羟基脂肪酸、以及它们的混合物。测试方法以下所述测定必须依次采用，使得本文描述并且受权利要求书保护的发明可被更完全理解。测试方法1-玻璃小瓶泡沫里程(mileage)方法玻璃小瓶泡沫里程测试方法的目的是测量在油脂污垢例如橄榄油存在下洗涤剂组合物的某种溶液产生的泡沫体积随时间的演变。此方法的步骤如下：1.通过随后在室温下向40ml玻璃小瓶(尺寸：95mmh×27.5mmd)加入以下物质的等分试样来制备测试溶液：a)10g指定洗涤剂浓度和水硬度的含水洗涤剂溶液，b)1.0g指定浓度和水硬度的含水蛋白质(或蛋白质混合物)溶液，和c)0.11g橄榄油(extravirginoliveoil(特级初榨橄榄油))。对于参考样品，用1.0ml去离子水替换蛋白质溶液。2.通过在磁力搅拌板(ika，型号rtcbs001；vwr磁力搅拌器，目录号58949-012；500rpm)上在室温下搅拌2分钟，然后手动摇动20秒并向上向下移动(每秒约2个上下循环，+/-30cm向上和30cm向下)，将测试溶液在密闭的试验瓶中混合。3.在摇动后，将密闭小瓶中的测试溶液在磁力搅拌板(ika，型号rtcbs001；vwr磁力搅拌器，目录号58949-012；500rpm)上在46℃水浴中进一步搅拌60分钟，以保持恒定温度。然后如上所述手动摇动样品另外20秒，用尺子记录初始泡沫高度(h1)。4.在46℃水浴中，在搅拌(ika，型号rtcbs001型；vwr磁力搅拌器，目录号58949-012；500rpm)的同时，再将样品温育30分钟，然后如上所述手动摇动20秒。记录最终泡沫高度(h2)。5.产生较大泡沫高度(h1和h2)的蛋白质溶液，优选与泡沫高度在h1和h2之间的较低液滴组合，是更理想的。测试方法2-小水槽泡沫里程方法当如下周期性地加入污垢负载时，可确定由液体洗涤剂组合物的溶液产生的泡沫体积的演变。将500ml液体洗涤剂组合物在15dh硬水中的溶液(最终浓度为0.12重量％，初始温度为46℃)的等分试样添加到圆柱形容器(尺寸：150mmd×150mmh)中。在测试期间将容器在水浴中温育以将溶液的温度保持在46℃和40℃之间。通过用位于容器中部的桨(尺寸：50mm×25mm)以135rpm机械搅拌120秒，在容器中产生初始泡沫体积。然后，用20ml注射器和自动泵(kdslegato110单注射器i/w泵)将0.5ml的油脂污垢的等分试样(组成：参见表3，0.5ml)添加到溶液中，同时桨以135rpm旋转至溶液中，持续14秒。混合后，在下一个循环之前将溶液再温育166秒。每180秒重复污垢注射、桨划和温育步骤，直到达到终点，并记录所需的污垢添加量。当连续两次或更多次观察到环绕叶轮至少半周的透明无泡沫环时，出现终点。将整个过程重复多次，并且计算每种液体洗涤剂组合物的所有平行测定的添加数的平均值。最后，然后如下计算泡沫里程指数：(测试液体洗涤剂组合物的污垢添加的平均数)/(参考液体洗涤剂组合物的污垢添加的平均数)×100。根据测试目的，本领域的技术人员可以选择替代的水硬度、溶液温度、产品浓度或污垢类型。表3-油脂污垢组合物成分重量％科瑞牌蔬菜油(criscooil)12.730科瑞牌起酥油(criscoshortening)27.752猪油7.638精制精炼食用牛油51.684油酸90％(techn)0.139棕榈酸，99+％0.036硬脂酸，99+％0.021测试方法3-大水槽泡沫里程方法当如下周期性地加入污垢负载时，可确定由洗涤剂组合物的溶液产生的泡沫体积的演变。用4巴的恒压将硬水(15dh)流填充到水槽(圆筒尺寸：300mmd×288mmh)中至4l。同时，通过移液管以0.67ml/秒的流速在水槽表面底部上方37cm的高度处分配洗涤剂组合物的等分试样(最终浓度0.12重量％)。由于水的压力，在水槽中产生初始泡沫体积。在测试期间，将溶液的温度保持在46℃。在记录初始泡沫体积(平均泡沫高度×水槽表面积)后，将固定量的油脂污垢(组成：参见表3，4ml)注入水槽的中部，同时桨(尺寸：10cm×5cm，以45度角定位在水槽的中部，处于气液界面处)以85rpm旋转20次到溶液中。该步骤后立即进行另一次总泡沫体积的测量。重复污垢注射、桨划和测量步骤，直到测得的泡沫体积达到设定为400cm3的最小水平。记录达到该水平所需的污垢添加量。将整个过程重复多次，并且计算每种洗涤剂组合物的所有平行测定的添加数的平均值。最后，然后如下计算泡沫里程指数：(测试洗涤剂组合物的污垢添加的平均数)/(参考洗涤剂组合物的污垢添加的平均数)×100。根据测试目的，本领域的技术人员可以选择替代的水硬度、溶液温度、产品浓度或污垢类型。实施例提供以下实施例以进一步说明本发明，而非解释为是对本发明的限制，因为在不脱离本发明实质或范围的条件下可以对本发明进行许多改变。实施例1a-铜绿假单胞菌菌株42a210s-dox铜绿假单胞菌10s-dox(seqidno:18)是氢过氧脂肪酸生成酶(油酸10s-脂氧合酶，ec1.13.11.77)，该氢过氧脂肪酸生成酶将不饱和脂肪酸(例如油酸和亚油酸)转化为对应的氢过氧化物质，并且其被包括作为本发明的一部分。设计并合成编码铜绿假单胞菌菌株42a210s-dox变体的密码子优化基因(seqidno:55)，包括含有his-tag、mbp标签和tev蛋白酶切割位点的n-末端氨基酸序列(seqidno:56)。基因合成后，用genscript(piscataway,nj)表达和纯化该蛋白质。简言之，将完整的合成基因序列亚克隆到pet28a载体中以进行异源表达。用重组质粒转化大肠杆菌bl21(de3)细胞，并将单菌落接种到含卡那霉素的lb培养基中。将培养物在15℃下以200rpm温育16小时，并加入异丙基β-d-1-硫代半乳糖苷(iptg)(最终浓度1mm)以诱导蛋白质表达。通过离心收获细胞，并通过超声处理来裂解沉淀。离心后，收集上清液，并使用镍亲和柱和本领域已知的标准工序通过一步纯化来纯化蛋白质。将蛋白质储存在含有50mmtris-hcl、150mmnacl和10％甘油的ph8.0的缓冲液中。通过bradford蛋白质测定以bsa为标准(thermofisher，目录号23236)进行测定，最终的蛋白质浓度为0.12mg/ml。实施例1b-点形念珠藻hpl点形念珠藻hpl(seqidno:40)是将氢过氧化物脂肪酸(例如10s-氢过氧亚油酸酯)转化为较小脂肪酸和醇并且被包括作为本发明的一部分的酶(氢过氧化物裂解酶，ec4.2.1.92)。设计并合成编码点形念珠藻hpl变体的密码子优化基因(seqidno:62)，包括含有his-tag、mbp标签和tev蛋白酶切割位点的n-末端氨基酸序列(seqidno:63)。基因合成后，用genscript(piscataway,nj)表达和纯化该蛋白质。简言之，将完整的合成基因序列亚克隆到pet28a载体中以进行异源表达。用重组质粒转化大肠杆菌bl21(de3)细胞，并在37℃下将单菌落接种到含卡那霉素的tb培养基中。当od600达到约0.8-1.0时，通过添加异丙基β-d-1-硫代吡喃半乳糖苷(iptg)(最终浓度0.1mm)和δ-氨基乙酰丙酸(最终浓度0.25mm)诱导蛋白质表达。将培养物在16℃下以200rpm培养16小时。通过离心收获细胞，并通过超声处理来裂解沉淀。离心后，收集上清液，并使用镍亲和柱和本领域已知的标准工序通过两步纯化来纯化蛋白质。将蛋白质储存在含有10％甘油的1xpbs缓冲液(ph7.4)中。通过bradford蛋白质测定以bsa为标准(thermofisher，目录号23236)进行测定，最终的蛋白质浓度为1.58mg/ml，通过在还原条件下考马斯蓝染色的sds-page凝胶的光密度分析估计，纯度为约75％。实施例1c-氢过氧脂肪酸生成和转化酶洗涤剂组合物在特定条件(例如，水硬度、溶液温度、洗涤剂浓度等)下，跟踪在污垢(即，橄榄油或油脂污垢)存在下由洗涤剂组合物的特定溶液所产生的泡沫体积的演变。制备下列溶液：a.硬水(15dh)：将0.75gmgcl2.6h2o(sigma-aldrich，目录号m9272)、2.10gcacl2.6h2o(sigma-aldrich，目录号21108)和0.689gnahco3(sigma-aldrich，目录号31437)溶解在5l去离子水中。b.对照参考洗涤剂组合物的洗涤剂溶液(“溶液dg-r”)使用fairydarkgreen(2017年2月在英国可商购获得)制备，稀释在上述制备的硬水(15dh)中，目标洗涤剂浓度为0.12％。c.蛋白质溶液：在进行泡沫里程方法之前，将蛋白质在去离子水中稀释至所需浓度。d.油脂污垢：根据表3中所述的组合物制备油脂污垢。实施例2：示例性手动盘碟洗涤洗涤剂组合物表5中示出包含根据本发明的a)氢过氧脂肪酸生成酶铜绿假单胞菌菌株42a210s-dox(seqidno:18)和b)一种或多种氢过氧化脂肪酸转化酶铜绿假单胞菌菌株42a27,10-ds/hp-异构酶(seqidno:38)或点形念珠藻hpl(seqidno:40)的手动盘碟洗涤洗涤剂组合物。酶可以按照实施例1a和1b中描述的方案或本领域中描述的类似方法来制备(estupinan,m.等人(2015)).plosone10(7):e0131462/0131461-e0131462/0131420)。表5：洗涤剂组合物自动盘碟洗涤组合物的实施例下列是自动盘碟洗涤组合物形式的本发明消费产品组合物的非限制性实施例。其中上表中的值以活性物质的克数给出。淀粉酶由novozymes提供的漂白活化剂weylchem提供的paanlutensolto7由basf提供的非离子表面活性剂液体聚合物由nipponshokubi提供的gt101mgda由basf提供的甲基甘氨酸二乙酸三钠蛋白酶由dupont提供的磺化聚合物由dowchemicals提供的acusol588slf180由basf提供的非离子表面活性剂洗发剂组合物的实施例下列是用于清洁毛发的洗发剂组合物形式的本发明消费产品组合物的非限制性实施例。1衍生自具有低含水粘度的天然胶的阳离子聚合物2阳离子合成共聚物3植物来源的阳离子聚合物实施例17至22：用于手洗或洗衣机(通常为顶部加载式洗衣机)的颗粒状衣物洗涤剂组合物。实施例23-28：通常用于前加载式自动洗衣机的颗粒状衣物洗涤剂组合物。实施例29-35：重垢型液体衣物洗涤剂组合物。基于总清洁和/或处理组合物重量。将酶水平报告为原料。实施例36至47：单位剂量组合物这些实施例提供了用于单位剂量衣物洗涤剂的各种配方，并且包括包含一个粉末隔室和一个液体隔室的双隔室单位剂量产品。用于包封组合物的膜为pva。通过将选自组合物a-e的液体隔室组合物与选自组合物f-k的粉末隔室组合物组合来制备每个实施例。实施例363738394041液体组合物15ga17gb20gc19gc15gc25gc固体组合物15gl14gf15gg18gh15gi12gj实施例424344454647液体组合物20gd18gd22gd32ge32ge27ge固体组合物20gk13gl15gf17gg12gh18gi下列实施例a-e是单位剂量产品的液体组合物：下列实施例f-k是单位剂量产品的粉末/固体组合物：基于总清洁和/或处理组合物/隔室重量。将酶水平报告为原料。对于上述实施例17-47，适用以下定义：ae1.8s为c12-15烷基乙氧基(1.8)硫酸盐ae3s为c12-15烷基乙氧基(3)硫酸盐ae7为c12-13醇乙氧基化物，平均乙氧基化度为7ae8为c12-13醇乙氧基化物，平均乙氧基化度为8ae9为c12-13醇乙氧基化物，平均乙氧基化度为9淀粉酶1为15mg活性物质/g淀粉酶2为29mg活性物质/g淀粉酶3为20mg活性物质/g，as为c12-14烷基硫酸盐纤维素酶2为cellucleantm，15.6mg活性物质/g木葡聚糖酶为20mg活性物质/g螯合剂1为二亚乙基三胺五醋酸螯合剂2为1-羟乙烷1,1-二膦酸螯合剂3为乙二胺-n,n′-二琥珀酸的钠盐，(s,s)异构体(edds)分散体b为糖苷水解酶，报告为1000mg活性物质/gdti1为聚(4-乙烯基吡啶-1-氧化物)(诸如)，dti2为聚(1-乙烯基吡咯烷酮-共-1-乙烯基咪唑)(诸如)。hsas为中间支化的烷基硫酸盐，如us6,020,303和us6,060,443中公开的las为具有c9-c15平均脂族碳链链长的直链烷基苯磺酸盐(hlas为酸形式)。脂肪酶为18mg活性物质/g甘露聚糖酶为25mg活性物质/g核酸酶为磷酸二酯酶seqidno1，报告为1000mg活性物质/g光学增白剂1为4,4′-双{[4-苯胺基-6-吗啉基-均-三嗪-2-基]-氨基}-2,2′-二苯乙烯二磺酸二钠光学增白剂2为4,4′-双-(2-磺基苯乙烯基)联苯二钠(钠盐)光学增白剂3为来自3vsigma的香料胶囊包封物为核-壳三聚氰胺甲醛香料微胶囊光漂白剂为磺化酞菁锌抛光酶为对硝基苄基酯酶，报告为1000mg活性物/g如本公开中所述的聚醚胺。聚合物1为双((c2h5o)(c2h4o)n)(ch3)-n+-cxh2x-n+-(ch3)-双((c2h5o)(c2h4o)n)，其中n＝20至30，x＝3至8，或其硫酸化或磺酸化变体聚合物2为乙氧基化(eo15)的四亚乙基五胺聚合物3为乙氧基化的聚乙烯亚胺聚合物4为乙氧基化的六亚甲基二胺聚合物5为acusol305，由rohm&haas提供聚合物6为接枝有乙酸乙烯酯侧链的聚乙二醇聚合物，由basf提供。蛋白酶为40.6mg活性物质/g蛋白酶2为32.89mg活性物质/g蛋白酶3为84mg活性物质/g季铵为c12-14二甲基羟乙基氯化铵s-acmc为由megazyme提供的活性蓝19偶氮-cm-纤维素去垢剂为sf2，由solvay提供结构剂为氢化蓖麻油紫罗兰dd为由milliken提供的噻吩偶氮聚合物调色染料针对酶给出的所有百分比和比率均基于活性蛋白质。除非另外指明，本文所有的百分比和比率都按重量计算。除非另外指明，否则所有百分比和比率均基于总组合物计算。应当理解，贯穿本说明书给出的每一最大数值限度包括每一较低数值限度，如同此类较低数值限度在本文中明确写出。贯穿本说明书给出的每一最小数值限度将包括每一较高数值限度，如同此类较高数值限度在本文中明确写出。贯穿本说明书给出的每一数值范围将包括落在此类较宽数值范围内的每一较窄数值范围，如同此类较窄的数值范围全部在本文中明确写出。本文所公开的量纲和值不应理解为严格限于所引用的精确数值。相反，除非另外指明，否则每个此类量纲旨在表示所述值以及围绕该值功能上等同的范围。例如，公开为“40mm”的量纲旨在表示“约40mm”。序列表<110>宝洁公司（procter&gamblecompany）velasquez,juane.gonzales,denisa.bettiol,jean-lucp.geary,nicholasw.<120>清洁具有包含脂肪酸的污垢的表面的方法以及用于该方法的消费产品组合物<130>15408<160>80<170>patentin3.5版<210>1<211>1165<212>prt<213>小麦全蚀病菌（gaeumannomycesgraminis）<400>1metthrvalserthrhishisaspaspserproglyleuserglyarg151015leuargaspleuleuhishisvalpheglyasnglnlysserprothr202530valtyrproasnalaproglyasnseralalysprovalprothrgly354045leualaaspaspileasplysleuglyphelysaspileaspthrleu505560leuilepheleuasnseralavallysglyvalasnaspaspglngln65707580pheleuleuglulysmetileglnleuleualalysleuproproala859095serarggluglylyslysleuthraspglyleuileasnaspleutrp100105110aspserleuasphisproprovalalaserleuglylysglypheser115120125phearggluproaspglyserasnasnasnilehisleuproserleu130135140glyalaalaasnthrprotyralaargserthrlysproleuvalphe145150155160glnasnproasnproproaspproalathrilepheaspthr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