用于变应原的选择性定量与检测的系统和方法与流程

本申请要求下列美国临时专利申请的申请日的权益：系列号62/035,968,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including GLY M BD 28 K”；系列号62/035,981,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including GLY M BD 30 K”；系列号62/035,997,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including Kunitz Trypsin Inhibitor 1”；系列号62/036,007,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including Kunitz Trypsin Inhibitor 3”；系列号62/036,016,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including GLY M 8(2S Albumin)”；系列号62/036,024,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including Lectin”；及系列号62/036,032,2014年8月11日提交，题为“Methods and Systems for Selective Quantitation and Detection of Allergens Including Lipoxygenase,”，每一个申请的披露内容通过引用整体并入本文。

背景

本领域当前优选用于分析植物中基因表达的方法包括基于DNA的技术(例如PCR和/或RT-PCR)；使用报告基因；Southern印迹；和免疫化学。这些方法都有各种缺点。在植物、植物部分和/或食品中检测已知和潜在的变应原是公共安全的一个重要课题。

质谱虽然先前已经公开，但是现有的手段是有限的，没有选择和灵敏的定量。仍然需要用于在植物、植物部分和/或食品中已知和/或潜在的变应原的选择的、灵敏的定量的高通量方法。

公开

本发明涉及利用生物信息学研究来鉴定候选特征肽，用于使用质谱法对来自植物，植物部分和/或食品的复杂蛋白质样品进行定量多重分析的方法和系统。提供了使用生物信息学方法选择候选特征肽用于定量的用途和方法。还提供了包括色谱和质谱的系统，用于利用所选出的特征肽。

在一个方面，提供了选择候选特征肽用于从基于植物的样品中定量已知的变应原和潜在的变应原的方法。该方法包括：

(a)基于与至少一种已知变应原蛋白质序列的同源性鉴定潜在变应原；

(b)进行所述至少一种已知变应原与步骤(a)中鉴定的潜在变应原的序列比对；

(c)基于所述序列比对选择共有序列或代表性序列；

(d)基于来自在步骤(c)中选择的共有序列或代表性序列的序列比对和计算机消化数据的保守区域或结构域，确定多个候选特征肽；和

(e)基于所述特征肽的测量(measurements)，定量所述基于植物的样品中的所述至少一种已知变应原和潜在变应原的量。

在一个实施方案中，所述定量步骤使用柱色谱和质谱。在另一个实施方案中，定量步骤包括使用高分辨率精确质量质谱(HRAM MS)测量所述多个候选特征肽。在另一个实施方案中，定量步骤包括从质谱计算候选特征肽的相应峰高度或峰面积。在另一个实施方案中，定量步骤包括将来自质谱法高碎裂模式的数据和低碎裂模式的数据进行比较。

在一个实施方案中，所述至少一种已知的变应原包含至少一种选自下组的变应原：Gly m 1，Gly m 3，Gly m 4，Gly m 5(β-伴大豆球蛋白)，Gly m 6(大豆球蛋白)Gly m 6(大豆球蛋白)G2，Gly m 6(大豆球蛋白)G3，Gly m 6(大豆球蛋白)G4，Gly m 6(大豆球蛋白)前体，Kunitz胰蛋白酶抑制剂1，Kunitz胰蛋白酶抑制剂3，Gly m Bd 28 K，Gly m Bd 30 K，Gly m 8(2S白蛋白)，凝集素和脂加氧酶。在另一个实施方案中，至少一种已知的变应原包括Gly m Bd 28 K、Gly m Bd 30 K、Kunitz胰蛋白酶抑制剂1、Kunitz胰蛋白酶抑制剂3、Gly m 8(2S白蛋白)、凝集素，或脂加氧酶。

在另一个实施方案中，步骤(c)的共有序列或代表性序列对于Gly m Bd28 K而言包含SEQ ID NO:21,28,29,30,31,32或33。在一个进一步的实施方案中，对于Gly m Bd 28 K，步骤(c)的共有序列或代表性序列包含SEQ ID NO:21或28。在另一个实施方案中，对于Gly m Bd 30 K，步骤(c)的共有序列或代表性序列包含SEQ ID NO:22、43、或44。在一个进一步的实施方案中，对于Gly m Bd 30 K，步骤(c)的共有序列或代表性序列包含SEQ ID NO:22或43。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，步骤(c)的共有序列或代表性序列包含SEQ ID NO:23、52、53、或54。在一个进一步的实施方案中，对于Kunitz胰蛋白酶抑制剂1，步骤(c)的共有序列或代表性序列包含SEQ ID NO:23或52。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，步骤(c)的共有序列或代表性序列包含SEQ ID NO:24、61、62、或63。在一个进一步的实施方案中，对于Kunitz胰蛋白酶抑制剂3，步骤(c)的共有序列或代表性序列包含SEQ ID NO:24或61。在另一个实施方案中，对于Gly m 8(2S白蛋白)，步骤(c)的共有序列或代表性序列包含SEQ ID NO:25、72、73、或74。在一个进一步的实施方案中，对于Gly m 8(2S白蛋白)，步骤(c)的共有序列或代表性序列包含SEQ ID NO:25。在另一个实施方案中，对于凝集素，步骤(c)的共有序列或代表性序列包含SEQ ID NO:26、82、83、84、85、86、87、88、89、或90。在一个进一步的实施方案中，对于凝集素，步骤(c)的共有序列或代表性序列包含SEQ ID NO:26或82。在另一个实施方案中，对于脂加氧酶，步骤(c)的共有序列或代表性序列包含SEQ ID NO:27、95、96、97、98、99、100、101、102、103、或104。在一个进一步的实施方案中，对于脂加氧酶，步骤(c)的共有序列或代表性序列包含SEQ ID NO:27或95。

在一个实施方案中，对于Gly m Bd 28 K，潜在的变应原包含选自SEQ ID NO:21和29-33的至少一个序列。在另一个实施方案中，对于Gly m Bd 30 K，潜在的变应原包含选自SEQ ID NOs:22和43-44的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，潜在的变应原包含选自SEQ ID NO:23和53-54的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，潜在的变应原包含选自SEQ ID NO:24和62-63的至少一个序列。在另一个实施方案中，对于Gly m 8(2S白蛋白)，潜在的变应原包含选自SEQ ID NO:25和72-74的至少一个序列。在另一个实施方案中，对于凝集素，潜在的变应原包含选自SEQ ID NO:26和83-90的至少一个序列。在另一个实施方案中，对于脂加氧酶，潜在的变应原包含选自SEQ ID NO:27和96-104的至少一个序列。

在另一个实施方案中，对于Gly m Bd 28 K，候选特征肽包含选自SEQ ID NO:9和34-42的至少一个序列。在另一个实施方案中，对于Gly m Bd 28 K，候选特征肽包含SEQ ID NO:34-42。在另一个实施方案中，对于Gly m Bd 30 K，候选特征肽包含选自SEQ ID NO:10和45-51的至少一个序列。在另一个实施方案中，对于Gly m Bd 30 K，候选特征肽包含SEQ ID NO:45-51。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，候选特征肽包含选自SEQ ID NO:7和55-60的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，候选特征肽包含SEQ ID NO:55-60。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，候选特征肽包含选自SEQ ID NO:8和64-71的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，候选特征肽包含SEQ ID NO:64-71。在另一个实施方案中，对于Gly m 8(2S白蛋白)，候选特征肽包含选自SEQ ID NO:11和75-81的至少一个序列。在另一个实施方案中，对于Gly m 8(2S白蛋白)，候选特征肽包含SEQ ID NO:75-81。在另一个实施方案中，对于凝集素，候选特征肽包含选自SEQ ID NO:91-94的至少一个序列。在另一个实施方案中，对于凝集素，候选特征肽包含SEQ ID NO:91-94。在另一个实施方案中，对于脂加氧酶，候选特征肽包含选自SEQ ID NO:105-120的至少一个序列。在另一个实施方案中，对于脂加氧酶，候选特征肽包含SEQ ID NO:105-120。在另一个实施方案中，基于植物的样品包含选自大豆种子或大豆种子的部分。

在另一方面，提供了用于在基于植物的样品中定量一种或多种具有已知氨基酸序列的目标蛋白质的系统。该系统包括：

(a)用于从基于植物的样品中提取蛋白质的高通量手段；

(b)用于以至少一种蛋白酶消化所提取的蛋白质的处理模块；

(c)用于在单个步骤中分离肽的分离模块；

(d)用于为至少一种已知的变应原和潜在的变应原选择多个特征肽的选择模块；和

(e)用于测量所述多个特征肽的质谱。

在一个实施方案中，分离模块包括柱色谱。在另一个实施方案中，柱色谱法包括液相柱色谱。在另一个实施方案中，质谱包括高分辨率精确质量质谱(HRAM MS)。在另一个实施方案中，选择模块使用本文提供的方法。

在一个实施方案中，基于植物的样品中的具有已知氨基酸序列的一种或多种目的蛋白质包含潜在的变应原。在另一个实施方案中，对于Gly m Bd 28 K，潜在的变应原包含选自SEQ ID NO:21和29-33的至少一个序列。在另一个实施方案中，对于Gly m Bd 30 K，潜在的变应原包含选自SEQ ID NO:22和43-44的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，潜在的变应原包含选自SEQ ID NO:23和53-54的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，潜在的变应原包含选自SEQ ID NO:24和62-63的至少一个序列。在另一个实施方案中，对于Gly m 8(2S白蛋白)，潜在的变应原包含选自SEQ ID NO:25和72-74的至少一个序列。在另一个实施方案中，对于凝集素，潜在的变应原包含选自SEQ ID NO:26和83-90的至少一个序列。在另一个实施方案中，对于脂加氧酶，潜在的变应原包含选自SEQ ID NO:27和96-104的至少一个序列。

在另一个实施方案中，对于Gly m Bd 28 K，候选特征肽包含选自SEQ ID NO:9和34-42的至少一个序列。在另一个实施方案中，对于Gly m Bd 28 K，特征肽包含SEQ ID NO:34-42。在另一个实施方案中，对于Gly m Bd 30 K，候选特征肽包含选自SEQ ID NO:10和45-51的至少一个序列。在另一个实施方案中，对于Gly m Bd 30 K，特征肽包含SEQ ID NO:45-51。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，候选特征肽包含选自SEQ ID NO:7和55-60的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂1，特征肽包含SEQ ID NO:55-60。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，候选特征肽包含选自SEQ ID NO:8和64-71的至少一个序列。在另一个实施方案中，对于Kunitz胰蛋白酶抑制剂3，特征肽包含SEQ ID NO:64-71。在另一个实施方案中，对于Gly m 8(2S白蛋白)，候选特征肽包含选自SEQ ID NO:11和75-81的至少一个序列。在另一个实施方案中，对于Gly m 8(2S白蛋白)，特征肽包含SEQ ID NO:75-81。在另一个实施方案中，对于凝集素，候选特征肽包含选自SEQ ID NO:91-94的至少一个序列。在另一个实施方案中，对于凝集素，特征肽包含SEQ ID NO:91-94。在另一个实施方案中，对于脂加氧酶，候选特征肽包含选自SEQ ID NO:105-120的至少一个序列。在另一个实施方案中，对于脂加氧酶，特征肽包含SEQ ID NO:105-120。在另一个实施方案中，基于植物的样品包含大豆种子或大豆种子的部分。

在另一方面，提供了在基于植物的样品中定量至少一种具有已知氨基酸序列的变应原和同源的潜在变应原的高通量方法。该方法包括使用本文提供的系统。

附图说明

图1示出了本文公开的方法和系统的代表性分析工作流程。

图2A-2J显示了下列选定的特征肽：SEQ ID NO:9 NKPQFLAGAASLLR；SEQ ID NO:34 LGFIYDDELAER；SEQ ID NO:35 TVVEEIFSK；SEQ ID NO:36 MMQDQEEDEEEK；SEQ ID NO:37 NAYGWSK；SEQ ID NO:38 ALHGGEYPPLSEPDIGVLLVK；SEQ ID NO:39 QGDVFVVPR；SEQ ID NO:40 YFPFCQVASR；SEQ ID NO:41 TLMGPELSAAFGVSEDTLR；SEQ ID NO:42 SFANDVVMDVF的代表性SRM LC-MS/MS，来自Gly m Bd 28 K的胰蛋白酶消化大豆样品色谱图。

图2K显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:9 NKPQFLAGAASLLR天然丰度肽和重同位素标记肽转换。

图2L显示Gly m Bd 28 K和Gly m Bd 28 K的潜在同源物之间的序列比对。

图3A-3H显示下列选定的特征肽：SEQ ID NO:10 GVITQVK；SEQ ID NO:45 SILDLDLTK；SEQ ID NO:46 FTTQK；SEQ ID NO:47 NNLNYIR；SEQ ID NO:48 FADITPQEFSK；SEQ ID NO:49 EQYSCDHPPASWDWR；SEQ ID NO:50 VTIDGYETLIMSDESTESETEQAFL SAILEQPISVSIDAK；和SEQ ID NO:51 NTGNLLGVCGMNYFASYPTK的代表性SRM LC-MS/MS，来自Gly m Bd 30 K的胰蛋白酶消化大豆样品色谱图。

图3I显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:10 GVITQVK天然丰度肽和重同位素标记肽转换。

图3J显示Gly m Bd 30 K和Gly m Bd 30 K的潜在同源物之间的序列比对。

图4A-4G显示下列选定的特征肽：SEQ ID NO:7 GGGIEVDSTGK；SEQ ID NO:55 EICPLTVVQSPNELDK；SEQ ID NO:56 EGLQAVK；SEQ ID NO:57 LVFCPQQAEDNK；SEQ ID NO:58 CEDIGIQIDDDGIR；SEQ ID NO:59 LVLSK；和SEQ ID NO:60 NKPLVVQFQK的代表性SRM LC-MS/MS，来自Kunitz胰蛋白酶抑制剂1的胰蛋白酶消化大豆样品色谱图。

图4H显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:7 GGGIEVDSTGK天然丰度肽和重同位素标记肽转换。

图4I显示Kunitz胰蛋白酶抑制剂1与Kunitz胰蛋白酶抑制剂1的潜在同源物之间的序列比对。

图5A-5I显示下列选定的特征肽：SEQ ID NO:8 GIGTIISSPYR；SEQ ID NO:64 CPLTVVQSR；SEQ ID NO:65 NELDK；SEQ ID NO:66 IGENK；SEQ ID NO:67 DAMDGWFR；SEQ ID 68 LVFCPQQAEDDK；SEQ ID NO:69 CGDIGISIDHDDGTR；SEQ ID NO:70 LVVSK；和SEQ ID NO:71 NKPLVVQFQK的代表性SRM LC-MS/MS，来自Kunitz胰蛋白酶抑制剂3的胰蛋白酶消化大豆样品色谱图。

图5J显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:8 GIGTIISSPYR天然丰度肽和重同位素标记肽转换。

图5K显示Kunitz胰蛋白酶抑制剂3和Kunitz胰蛋白酶抑制剂3的潜在同源物之间的序列比对。

图6A-6H显示下列选定的特征肽：SEQ ID NO:11IMENQSEELEEK；SEQ ID NO:75 WQHQQDSCR；SEQ ID NO:76 QLQGVNLTPCEK；SEQ ID NO:77 HIMEK；SEQ ID NO:78 DEDEEEEGHMQK；SEQ ID NO:79 CCTEMSELR；SEQ ID NO:80 ELINLATMCR；和SEQ ID NO:81 FGPMIQCDLSSDD的代表性SRM LC-MS/MS，来自Gly m 8(2S白蛋白)的胰蛋白酶消化大豆样品色谱图。

图6I显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:11 IMENQSEELEEK天然丰度肽和重同位素标记肽转换。

图6J显示Gly m 8(2S白蛋白)和Gly m 8的潜在同源物之间的序列比对。

图7A-7D显示下列选定的特征肽：SEQ ID NO:91VFSPNK；SEQ ID NO:92 ANSTNTVSFTVSK；SEQ ID NO:93 QQNLIFQGDAAISPSGVLR；和SEQ ID NO:94 TADGLAFFLAPVGSKPQSK的代表性SRM LC-MS/MS，来自凝集素的胰蛋白酶消化大豆样品色谱图。

图7E显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:91 VFSPNK天然丰度肽和重同位素标记肽转换。

FIG 7F显示凝集素和凝集素的潜在同源物之间的序列比对。

图8A-8P显示下列选定的特征肽：SEQ ID NO:105 SSDFLTYGIK；SEQ ID NO:106 GTVVLMPK；SEQ ID NO:107 NVLDFNAITSIGK；SEQ ID NO:108 GGVIDTATGILGQGVSLVGGVIDTATSFLGR；SEQ ID NO:109 IFFVNDTYLPSATPAPLLK；SEQ ID NO:110 DENFGHLK；SEQ ID NO:111 SLSHDVIPLFK；SEQ ID NO:112 SLYEGGIK；SEQ ID NO:113 TDGENVLQFPPPHVAK；SEQ ID NO:114 INSLPTAK；SEQ ID NO:115 TILFLK；SEQ ID NO:116 HLSVLHPIYK；SEQ ID NO:117 QSLINADGIIEK；SEQ ID NO:118 FIPAEGTPEYDEMVK；SEQ ID NO:119 ALEAFK；和SEQ ID NO:120 GIPNSISI的代表性SRM LC-MS/MS，来自胰蛋白酶消化大豆样品色谱图。

图8Q显示代表性SRM LC-MS/MS标准色谱图-500ng/mL合成肽SEQ ID NO:105 SSDFLTYGIK天然丰度肽和重同位素标记肽转换。

图8R显示脂加氧酶和脂加氧酶的潜在同源物之间的序列比对。

具体实施方式

启用能够选择性地检测目标蛋白质和测量目标蛋白质水平的灵敏多重测定具有重要的意义。目前，相关的蛋白质表达检测的技术严重依赖于传统的免疫化学技术，如何应对该技术中每个样品要求产生的数据量成为了一项挑战。

大豆是价值数以十亿美元计的贸易品，这是由于其平衡蛋白质、淀粉、油组成——按重量比2:2:1。许多种子，包括大豆，所含的某些蛋白质是变应原和抗营养因子。因此有人担忧，在遗传修饰的大豆品种中，与通过传统育种开发的品种相比，变应原水平潜在可能改变。迄今为止作物中的变应原水平几乎完全是通过免疫测定法，例如酶联免疫吸附测定(ELISA)或IgE-免疫印迹等来测量的；然而，这些方法的问题是灵敏度和特异性有限，且变化性高。

最近，人们对开发基于LC-MS/MS的方法以在单一分析中定量几种植物表达的蛋白质产生了兴趣。使用这些“特征肽”进行的分析包括通过定量目标蛋白质的几个高度特异性消化片段来跟踪蛋白质表达水平。这通常可以使用与选择反应监测(SRM)串联质谱联用的液相色谱法来完成。本文提供了改进的多重LC-MS/MS方法和系统，其使得在转基因和非转基因大豆中同时定量几种变应原蛋白成为可能。本文提供的方法和系统就包括准确度、精确度、线性、检测限和定量限等在内的分析优值(analytical figures of merit)，以及包括样品通量、可转移性和易用性在内的其他考虑因素得到了验证。可以使用复用型式(multiplexing format)来定量变应原，并且可以例如在一天(二十四小时)窗口(从田间到测量的数值)内从田间收获样品、处理和分析/定量。此外，所提供的方法和系统的样品制备可以完全规模放大以实现高通量，因此能够在单一批次中分析数百个样品。

代表性的大豆变应原包括例如Gly m 1，Gly m 3，Gly m 4，Gly m 5(β-伴大豆球蛋白)，Gly m 6(大豆球蛋白)G1，Gly m 6(大豆球蛋白)G2，Gly m 6)G3，Gly m 6(大豆球蛋白)G4，Gly m 6(大豆球蛋白)前体，Gly m 6(大豆球蛋白)G4前体，Kunitz胰蛋白酶抑制剂1，Kunitz胰蛋白酶抑制剂3，Gly m Bd 28 K，Gly m Bd 30 K，Gly m 8(2S白蛋白)，凝集素和脂加氧酶。

代表性的小麦变应原包括例如前纤维蛋白(Tri a12)，小麦脂质转移蛋白1(Tri a14)，凝集素同工凝集素1(Tri a18)，ω-5麦醇溶蛋白-种子储存蛋白(Tri a19)，麦醇溶蛋白(Tri a20；NCBI登录号M10092，M11073，M11074，M11075，M11076，K03074和K03075)，硫氧还蛋白(Tri a25)，高分子量麦谷蛋白(Tri a26)，低分子量麦谷蛋白(Tri a36)，和α-嘌呤硫堇(purothionin)(Tri a37)。

代表性的玉米变应原包括例如玉米脂转移蛋白(LTP)(Zea m14)和硫氧还蛋白(Zea m25)。

代表性的玉米变应原包括，例如，水稻前纤维蛋白A(Ory s12)。

在一些实施方案中，所提供的方法和系统使用偶联于串联质谱的液相色谱(LC-MS/MS)检测来自大豆的十六种不同变应原的蛋白质表达水平。在一些实施方案中，所述方法和系统能够分析单独的变应原，或分析每种变应原与其它蛋白质的组合以进行多重测定，用于植物基质中的定性和定量分析。

在一些实施方案中，用于定量研究的质谱检测可以通过在三重四极杆质谱仪上进行选择反应监测来实现。使用这种类型的仪器设备，对在源中形成的目标离子(肽)的初始质量选择，随后在MS的碰撞区域中解离该前体离子，然后是特定产物(子)离子的质量选择和计数。在一些实施方案中，用于定量研究的质谱检测可以使用选择反应监测(SRM)来完成。使用特定类型的仪器设备，在源中形成的目标离子的初始质量选择，随后在质谱仪(MS)的碰撞区域中解离该前体(蛋白质)离子，然后是特定产物(肽)离子的质量选择和计数。在一些实施方案中，单位时间的计数可以提供可积分的峰面积，从中可以确定分析物的量或浓度。在一些实施方案中，在具有HRAM能力的质谱仪上进行的定量用高分辨率精确质量(HRAM)监测可以包括但不限于混合四极-飞行时间、四极-轨道阱、离子阱-轨道阱、或四极-离子阱-轨道阱(三栅)质谱仪。使用特定类型的仪器设备，肽不经历碎裂条件，而是作为完整肽使用全扫描或靶向扫描模式(例如选择性离子监测模式或SIM)测量。可以通过为每个特定分析物产生提取离子色谱图来确定可积分的峰面积，并且可以计算分析物的量或浓度。数据的高分辨率和精确质量性质使得目标分析物(蛋白质和/或肽)的高度特异性和灵敏的离子信号成为可能。

除非另有说明，本申请中，包括说明书和权利要求书中使用的以下术语，具有下面给出的定义。必须注意，如说明书和所附权利要求中所使用的，单数形式“一”，“一个”和“该”包括复数指示物，除非上下文另有明确指示。

如本文所用，术语“生物限制”是指限制遗传修饰的植物或其遗传物质运动到指定区域。该术语包括物理的、物理化学的、生物学的限制，以及其他形式的可防止遗传修饰的植物在自然环境或人工生长条件中的存活、扩散或繁殖的限制。

如本文所用，术语“复杂蛋白质样品”用于区分样品与纯化的蛋白质样品。复杂蛋白样品含有多种蛋白质，并可另外含有其他污染物。

如本文所用的，通用术语“质谱”或“MS”是指任何合适的质谱方法、装置或配置，包括例如电喷雾电离(ESI)、基质辅助激光解吸/电离(MALDI)MS、MALDI-飞行时间(TOF)MS、大气压(AP)MALDI MS、真空MALDI MS、或其组合。质谱装置通过测量分子通过一组磁场和电场的飞行路径来测量分子的分子量(作为分子的质荷比的函数)。质荷比是在带电粒子的电动力学中广泛使用的物理量。特定肽的质荷比可以由本领域技术人员先验地计算。具有不同质荷比的两个粒子当受到相同的电场和磁场时，在真空中不会在相同的路径中移动。

质谱仪器由三个模块组成：离子源，其将样品分子分离成离子；质量分析器，其通过施加电磁场来按照质量对离子进行分类；和检测器，其测量指示物的量的值，并因此提供用于计算每种离子存在的丰度的数据。该技术可以应用于定性和定量。这些包括鉴定未知化合物，确定分子中元素的同位素组成，通过观察化合物碎裂来确定其结构，以及定量样品中化合物的量。

质谱方法和装置的详细概述可以在以下参考文献中找到，这些通过引用并入本文：Can和Annan(1997)Overview of peptide and protein analysis by mass spectrometry的潜在同源物之间的序列比对。收录于Current Protocols in Molecular Biology，Ausubel等人编，New York:Wiley,p.10.21.1-10.21.27；Paterson和Aebersold(1995)Electrophoresis 16:1791-1814；Patterson(1998)Protein identification and characterization by mass spectrometry.收录于Current Protocols in Molecular Biology，Ausubel等人编，New York:Wiley,p.10.22.1-10.22.24；和Domon和Aebersold(2006)Science 312(5771)：212-17。

如本文所用的术语，当同一样品中存在两种或更多种目的蛋白质和/或肽时，蛋白质和/或肽是“多重的”。

如本文所使用的，“植物性状”可以指植物的任何单一特征或可量化的度量。

如本文所用，短语“肽”或“肽”可以指α-氨基酸以确定的顺序连接而形成的短聚合物。肽也可以通过用蛋白酶消化多肽例如蛋白质产生。

如本文所使用的，短语“蛋白质”可以指由排列在直链中并通过相邻氨基酸残基的羧基和氨基之间的肽键连接在一起的氨基酸构成的有机化合物。蛋白质中的氨基酸序列由基因的序列定义，基因的序列以遗传密码编码。通常，遗传密码指定20个标准氨基酸，然而在某些生物体中，遗传密码可包括硒代半胱氨酸，在某些古细菌中，可包括吡咯赖氨酸。通常观察到蛋白质中的残基通过翻译后修饰作用被化学修饰，翻译后修饰作用可以在该蛋白质被细胞使用之前发生，或作为控制机制的一部分发生。蛋白质残基也可以根据本领域技术人员熟悉的技术通过设计加以修饰。如本文所用的，术语“蛋白质”涵盖包含天然存在的氨基酸、合成氨基酸、修饰的氨基酸或任何或所有上述的组合的直链。

如本文所使用的，术语“单次注射”是指MS或LC-MS装置操作中的初始步骤。当蛋白质样品在单次注射中被引入装置时，整个该样品是在单个步骤中引入的。

如本文所用，短语“特征肽”是指特定蛋白质的标识(短肽)序列。任何蛋白质可以含有平均10至100种特征肽。通常特征肽具有以下标准中的至少一种：容易通过质谱法检测；可预测且稳定地从液相色谱(LC)柱洗脱；通过反相高效液相色谱(RP-HPLC)富集；良好电离；良好碎裂；或上述者的组合。容易通过质谱法定量的肽通常具有以下标准中的至少一种：易于合成，能够被高度纯化(>97％)，可溶于≤20％乙腈，低非特异性结合，抗氧化，抗合成后修饰，疏水性或疏水性指数≥10且≤40。疏水性指数可以根据Krokhin，Molecular and Cellular Proteomics 3(2004)908计算，其通过引用并入本文。已知疏水性指数小于10或大于40的肽可能无法通过RP-HPLC柱可重复地分开或洗脱。

如本文所用的，术语“堆叠”是指掺入到植物基因组中的多个异源多核苷酸的存在。

串联质谱：在串联质谱中，从目标分子产生的母离子可以在质谱仪中被过滤，并且母离子随后被碎裂以产生一个或多个子离子，子离子随后在第二个质谱程序中被分析(检测和/或定量))。在一些实施方案中，排除串联质谱的使用。在这些实施方案中，在所提供的方法和系统中不使用串联质谱。因此，在这些实施方案中既不产生母离子也不产生子离子。

如本文所用的，术语“转基因植物”可以指在其基因组内包含异源多核苷酸的植物。通常，异源多核苷酸稳定整合在基因组内，使得多核苷酸被传递给后续世代。异源多核苷酸可以单独地或作为重组表达盒的一部分整合到基因组中。“转基因”在本文中用于包括任何这样的细胞、细胞系、愈伤组织、组织、植物部分或植物，其基因型已被异源核酸的存在改变，包括最初如此改变的那些转基因植物、以及从最初的转基因植物通过有性杂交或无性繁殖而生成的转基因植物。

在本发明的实施中可以处理任何可提供有用的植物部分的植物。实例包括提供花、水果、蔬菜和谷物的植物。

如本文所用，短语“植物”包括双子叶植物和单子叶植物。双子叶植物的实例包括烟草，拟南芥，大豆，番茄，木瓜，卡诺拉，向日葵，棉花，苜蓿，马铃薯，葡萄，木豆，豌豆，芸苔，鹰嘴豆，甜菜，油菜，西瓜，甜瓜，胡椒，花生，南瓜，萝卜，菠菜，倭瓜，西兰花，卷心菜，胡萝卜，花椰菜，芹菜，大白菜，黄瓜，茄子和莴苣。单子叶植物的实例包括玉米，水稻，小麦，甘蔗，大麦，黑麦，高粱，兰花，竹子，香蕉，香蒲，百合，燕麦，洋葱，小米和黑小麦。水果的实例包括香蕉，菠萝，橙子，葡萄，葡萄柚，西瓜，甜瓜，苹果，桃，梨，猕猴桃，芒果，油桃，番石榴，柿子，鳄梨，柠檬，无花果，和浆果。花的例子包括满天星，康乃馨，大丽花，水仙，天竺葵，非洲菊，百合，兰花，牡丹，野胡萝卜(Queen Anne’s lace)，玫瑰，金鱼草或其他插花或装饰花，盆花和花球。

在质谱方法中从复杂样品中鉴定单个蛋白质所允许的特异性是独特的，因为鉴定目标蛋白质仅需要目标蛋白质的序列。与其他形式的多重化相比，质谱法的独特之处是能够利用蛋白质的一级氨基酸序列的全长来靶向蛋白质的一级氨基酸序列的唯一标识类型部分，从而几乎消除非特异性检出。在本发明的一些实施方案中，利用独特地标识目标蛋白质的蛋白水解片段或一组蛋白水解片段来检测复杂蛋白质样品中的目标蛋白质。

在一些实施方案中，所公开的方法能够通过单次质谱分析来定量复杂蛋白质样品中的多种蛋白质或测定其比例，而不是分别多次测量每种目标蛋白质，再将单独的结果汇编成一个样品结果。

在一些实施方案中，本公开内容还提供了可用于开发和利用转基因植物技术的方法。具体地，公开的方法可以用于维持转基因植物的基因型经历连续世代。此外，本文公开的方法的一些实施方案可用于提供对如下所述的非转基因植物的高通量分析：该非转基因植物处于被来自邻近植物的转基因污染(例如通过异花授粉)的风险中。通过这些实施方案，可以促进和/或完成转基因的生物限制。在其它实施方案中，本文公开的方法可用于以高通量方式筛选植物转化程序的结果，以鉴定表现出期望的表达特征的转化体.

质荷比可以使用四极分析器来确定。例如，在“四极”或“四极离子阱”仪器中，振荡射频场中的离子受到与施加在电极之间的DC电势、RF信号的振幅、以及m/z成比例的力。可以选择电压和振幅，使得只有具有特定m/z的离子可以行进四极杆的全长，而所有其他离子被偏转。因此，四极仪器可以充当注入仪器中的离子的“质量过滤器”和“质量检测器”。

碰撞诱导解离(“CID”)通常用于产生用于进一步检测的子离子。在CID中，母离子通过与惰性气体(例如氩气)碰撞而获得能量，并且随后通过称为“单分子分解”的过程而碎裂。母离子中必须储存足够的能量，使得离子内的某些键由于能量增加而断裂。

质谱仪通常为用户提供离子扫描；即在给定范围(例如10至1200amu)内每个m/z的相对丰度。分析物测定的结果，即质谱，可以通过许多本领域已知的方法与原始样品中的分析物的量关联。例如，鉴于取样和分析参数是被仔细控制的，可以将给定离子的相对丰度与将该相对丰度转化为原始分子的绝对量的表进行比较。或者，可以与样品一起运行分子标准(例如，内标和外标)，并基于从那些标准产生的离子构建标准曲线。使用这种标准曲线，给定离子的相对丰度可以转化为原始分子的绝对量。许多其它用于将离子的存在或量与原始分子的存在或量相关联的方法是本领域普通技术人员公知的。

电离方法的选择可以基于待测量的分析物、样品类型、检测器类型、阳性对阴性模式的选择等来确定。离子可以使用多种方法产生，包括但不限于：电子电离，化学电离，快速原子轰击，场解吸和基质辅助激光解吸电离(MALDI)，表面增强激光解吸电离(SELDI)，解吸电喷雾离子化(DESI)，光子电离，电喷雾离子化和电感耦合等离子体。电喷雾离子化是指这样的方法，其中令溶液通过一段短的毛细管，管的末端施加高的正或负电位。到达管末端的溶液在溶剂蒸气中被蒸发(雾化)成非常小的溶液液滴的射流或喷雾。这种液滴的雾流过蒸发室，蒸发室被加热以防止冷凝并蒸发溶剂。随着液滴变小，电表面电荷密度增加，直到这样的时间到来：相同电荷之间的自然排斥导致离子和中性分子被释放。

LC的流出物可以直接且自动地(即，“在线”)注入到电喷雾装置中。在一些实施方案中，LC流出物中包含的蛋白质首先通过电喷雾离子化成母离子。

各种不同的质量分析器可用于液相色谱-质谱联用(LC-MS)。示例性质量分析器包括但不限于单四极杆、三重四极杆、离子阱、TOF(飞行时间)和四极杆飞行时间(Q-TOF)。

四极质量分析器可以由4个彼此平行设置的圆棒组成。在四极杆质谱仪(QMS)中，四极杆是仪器中负责基于质荷比(m/z)过滤样品离子的组成部分。基于离子在施加到杆上的振荡电场中的轨迹的稳定性，在四极中分离离子。

离子阱是电场或磁场的组合，可捕获真空系统或管的某个区域中的离子。在操作离子的量子态时，可以在质谱中使用离子阱。

飞行时间质谱法(TOFMS)是一种质谱方法，其中通过时间测量来确定离子的质荷比。离子通过已知强度的电场加速。该加速导致离子具有与任何其它具有相同电荷的离子相同的动能。离子的速度取决于质荷比。测量粒子随后到达相距已知距离的检测器所花费的时间。这个时间将取决于粒子的质荷比(越重的粒子达到的速度越低)。从这个时间和已知的实验参数，可以求出离子的质荷比。

在一些实施方案中，由所提供的方法和/或系统使用的特定仪器可以包括高碎裂模式和低碎裂模式(或可选地，非碎裂模式)。这样的不同模式可以包括交替扫描高能量和低能量获取方法以产生高分辨率质量数据。在一些实施方案中，高分辨率质量数据可以包括产物数据集(例如在高碎裂模式下从产物离子(碎片离子)得到的数据)和前体数据集(例如在低碎裂或非碎裂模式下从前体离子(未碎裂离子)得到的数据)。

在一些实施方案中，所提供的方法和/或系统使用质谱仪，质谱仪包括可用于选择步骤的过滤装置、可用于碎裂步骤的碎裂装置、和/或可用于获取和/或质谱创建步骤中的一个或多个质量分析器。

过滤装置和/或质量分析器可以包括四极。选择步骤和/或获取步骤和/或质谱创建步骤可涉及使用分辨四极(resolving quadrupole)。另外或替代地，过滤装置可包括二维或三维离子阱或飞行时间(ToF)质量分析器。该一个或多个质量分析器可以包括、或可以进一步包括下述的一种或多种：飞行时间质量分析器和/或离子回旋共振质量分析器和/或轨道阱质量分析器和/或二维或三维离子阱。

借助于基于质荷比(m/z)的选择的过滤可以通过使用能够基于m/z选择离子的质量分析器(例如四极杆)来实现；或传输宽的m/z范围，根据离子的m/z分离离子，然后通过离子的m/z值选择目标离子。后者的示例是与计时离子选择器组合的飞行时间质量分析器。所提供的方法和/或系统可包括使用色谱技术，例如液相色谱(LC)，分离和/或分开一种或多种目的蛋白质，例如从多种蛋白质中的两种或多种中分离和/或分开。该方法可以进一步包括测量目标蛋白质的洗脱时间和/或将测量的洗脱时间与预期的洗脱时间进行比较。

另外或可选地，可以使用离子迁移率技术分离目标蛋白质，其可以使用离子迁移小室进行。另外，可以通过离子迁移率漂移的次序或时间来选择目标蛋白质。该方法还可以包括测量感兴趣蛋白质的漂移时间和/或将测量的漂移时间与预期漂移时间进行比较。

在一些实施方案中，所提供的方法和/或系统是无标记的，其中定量可以通过比较注射之间和各样品之间的目标前体或产物m/z值的峰强度或质谱峰下面积来实现。在一些实施方案中，可以利用内标标准化来应对任何已知的相关分析误差。另一种无标记的定量方法，谱图计数(spectral counting)，包括以非冗余或冗余方式对每个给定肽获得的碎片离子谱或扫描的数目求和。然后将每种蛋白质的相关肽质谱相加，从而提供每种蛋白质的扫描数的度量，其与该蛋白质的丰度成比例。然后可以在样品/注射之间进行比较。

在一些实施方案中，离子源选自：(1)电喷雾离子化(“ESI”)离子源；(2)大气压光电离(“APPI”)离子源；(3)大气压化学电离(“APCI”)离子源；(4)基质辅助激光解吸电离(“MALDI”)离子源；(5)激光解吸电离(“LDI”)离子源；(6)大气压电离(“API”)离子源；(7)硅上的解吸电离(“DIOS”)离子源；(8)电子轰击(“E1”)离子源；(9)化学电离(“CI”)离子源；(10)场电离(“F1”)离子源；(11)场解吸(“FD”)离子源；(12)电感耦合等离子体(“ICP”)离子源；(13)快速原子轰击(“FAB”)离子源；(14)液体二次离子质谱(“LSIMS”)离子源；(15)解吸电喷雾离子化(“DESI”)离子源；(16)镍-63放射性离子源；(17)大气压基质辅助激光解吸电离离子源；和(18)热喷射离子源。

在一些实施方案中，所提供的方法和/或系统包括被配置为执行计算机程序元件的装置和/或控制系统，所述计算机程序元件包括用于令处理器执行用于实现所述方法的过程的计算机可读程序代码手段。

在一些实施方案中，所提供的方法和/或系统使用交替的低能量和高能量扫描功能与液相色谱分离植物提取物组合。可以提供目标蛋白质的信息列表，包括但不限于前体离子的m/z，产物离子的m/z，保留时间，离子迁移率漂移时间和迁移率变化率。在LC分离过程中，和当靶离子洗脱到质谱仪中时(以及当检测到低能前体离子或检测到高能量产物离子或者激活保留时间窗时)，所以方法的质量分析器和/或系统可以选择窄的m/z范围(具有可变化和可改变的宽度)，以将离子传递到气室。因此，可以显著增强信噪比以用于目标蛋白质的定量。

在一些实施方案中，在目标靶蛋白即将洗脱到质谱仪离子源中时的色谱保留时间，所提供的方法和/或系统的质量分析器可以根据靶前体例子选择窄的m/z范围(具有可变化和可改变的宽度)。然后将这些选定的离子转移到能够通过在高碎裂模式和低碎裂模式(或非碎裂模式)之间交替和重复切换来离解离子的仪器级，在所述高碎裂模式下样品前体离子基本上被碎裂成产物离子，在所述低碎裂模式下样品前体离子基本上不碎裂。通常在两种模式下均获得高分辨率的、准确的质谱，并且在实验结束时，通过其色谱洗脱时间和任选的其他物理化学性质的拟合紧密程度来识别相关的前体和产物离子。与目标靶蛋白相关的前体离子或产物离子的信号强度可用于确定植物提取物中蛋白质的量。

本领域技术人员将理解，在所提供的公开的基础上可以存在某些变化。因此，给出以下实施例用于说明本发明的目的，而不应解释为对本发明或权利要求的范围的限制。

实施例

实施例1

所提供的方法和系统用于测定大豆种子中的内源性大豆变应原蛋白，包括Gly m 1，Gly m 3，Gly m 4，Gly m 5(β-伴大豆球蛋白)，Gly m 6，Kunitz胰蛋白酶抑制剂1，Kunitz胰蛋白酶抑制剂3，Gly m Bd 28 K，Gly m Bd 30 K和Gly m 8(2S白蛋白)。

将100±0.5mg研磨的大豆种子样品用己烷脱脂两次并干燥，然后用含有5M尿素，2M硫脲，50mM Tris pH 8.0和65mM DTT的提取缓冲液提取。将样品在水浴中超声处理30分钟，涡旋1分钟，超声处理另外30分钟，并在4℃以>3,000rpm离心10分钟。

收集水性上清液并用提取缓冲液稀释以使内源性大豆变应原蛋白浓度达到校准标准范围。将稀释的提取物在95℃下与外加的1M Tris pH 8.0，0.5M DTT和去离子水一起变性20分钟，随后在4℃下冷藏10分钟。变性的提取物在37℃下与0.5mg/mL胰蛋白酶温育过夜(15小时)。消化反应用甲酸水(50/50v/v)淬灭并在4℃以>3,000rpm离心分钟。将消化的提取物的一份等分试样转移到自动进样器小瓶中，并与校准标准品一起分析液相色谱连同正离子电喷雾(ESI)串联质谱(LC-MS/MS)进行分析。特征肽的校准标准品如表1所列制备。

在该实施例中，内源性大豆变应原的检测限(LOD)和定量限(LOQ)列于表2，其中LOD和LOQ代表蛋白浓度(ng/mg)。

变应原的浓度从特征肽的定量(例如用于LC-MS/MS的Analyst Bioanalytical软件)计算，并通过包括酶联免疫吸附测定(ELISA)的其它方法验证。使用统计分析比较来自不同样品的变应原的计算浓度，结果显示了样品之间的良好一致性。

实施例2

从包括NCBI，Phytozome和UniProt在内的公共数据库中鉴定出几种Gly m Bd 28K的同源蛋白质序列。对鉴定的序列(SEQ ID NO:21和29-33)使用生物信息学工具加以分析，以鉴定可用的蛋白质序列之间的序列同源性和共有序列组成(参见图2L)。具体地，这涉及使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，将其在计算机上(in silico)消化，产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度来选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，所选择的特征肽的定量不仅可以测量Gly m Bd 28 K本身，还可以测量与Gly m Bd 28 K高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于Gly m Bd 28 K(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选择了数个特征肽(SEQ ID NO:9 NKPQFLAGAASLLR；SEQ ID NO:34 LGFIYDDELAER；SEQ ID NO:35 TVVEEIFSK；SEQ ID NO:36 MMQDQEEDEEEK；SEQ ID NO:37 NAYGWSK；SEQ ID NO:38 ALHGGEYPPLSEPDIGVLLVK；SEQ ID NO:39 QGDVFVVPR；SEQ ID NO:40 YFPFCQVASR；SEQ ID NO:41 TLMGPELSAAFGVSEDTLR；SEQ ID NO:42 SFANDVVMDVF)，并且这些特征肽的代表性定量显示于图2A-2J中。为SEQ ID NO:9 NKPQFLAGAASLLR合成了肽标准品用于定量和定性分析(参见图2K)。合成肽可以直接作为蛋白质定量的分析参考标准。

实施例3

从包括NCBI，Phytozome和UniProt在内的公共数据库中鉴定出Gly m Bd30 K的几个同源蛋白质序列。使用生物信息学工具分析鉴定出的序列(SEQ ID NO:22和43-44)以鉴定可用的蛋白质序列之间的序列同源性和共有序列组成(参见图3J)。具体地，这包括使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，对其进行计算机上消化以产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，选定的特征肽的定量不仅可以测量Gly m Bd 30 K本身，而且可以测量与Gly m Bd 30 K高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于Gly m Bd 30 K(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选择了数个特征肽(SEQ ID NO:10 GVITQVK；SEQ ID NO:45 SILDLDLTK；SEQ ID NO:46 FTTQK；SEQ ID NO:47 NNLNYIR；SEQ ID NO:48 FADITPQEFSK；SEQ ID NO:49 EQYSCDHPPASWDWR；SEQ ID NO:50 VTIDGYETLIMSDESTESETEQAFLSAILEQPISVSIDAK；和SEQ ID NO:51 NTGNLLGVCGMNYFASYPTK)，并且这些特征肽的代表性定量显示于图3A-3H中。为SEQ ID NO:10 GVITQVK合成了肽标准品用于定量和定性分析(参见图3I)。合成肽可以直接作为蛋白质定量的分析参考标准。

实施例4

从包括NCBI，Phytozome和UniProt在内的公共数据库中鉴定出Kunitz胰蛋白酶抑制剂1的几个同源蛋白质序列。使用生物信息学工具分析鉴定出的序列(SEQ ID NO:23和53-54)以鉴定可用的蛋白质序列之间的序列同源性和共有序列组成(参见图4I)。具体地，这涉及使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，对其进行计算机上消化以产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，所选择的特征肽的定量不仅可以测量Kunitz胰蛋白酶抑制剂1本身，而且还可以测量与Kunitz胰蛋白酶抑制剂1高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于Kunitz胰蛋白酶抑制剂1(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选择出几种特征肽(SEQ ID NO:7 GGGIEVDSTGK；SEQ ID NO:55 EICPLTVVQSPNELDK；SEQ ID NO:56 EGLQAVK；SEQ ID NO:57 LVFCPQQAEDNK；SEQ ID NO:58 CEDIGIQIDDDGIR；SEQ ID NO:59 LVLSK；和SEQ ID NO:60 NKPLVVQFQK)，并且这些特征肽的代表性定量显示在图4A-4G中。为SEQ ID NO:7 GGGIEVDSTGK合成了肽标准品用于定量和定性分析(见图4H)。合成肽可以直接作为蛋白质定量的分析参考标准。

实施例5

从包括NCBI，Phytozome和UniProt在内的公共数据库中鉴定出Kunitz胰蛋白酶抑制剂3的几个同源蛋白质序列。使用生物信息学工具分析鉴定出的序列(SEQ ID NO:24和62-63)以鉴定可用的蛋白质序列之间的序列同源性和共有序列组成(参见图5K)。具体地，这涉及使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，对其进行计算机上消化以产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，所选择的特征肽的定量不仅可以测量Kunitz胰蛋白酶抑制剂3本身，还可以测量与Kunitz胰蛋白酶抑制剂3高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于Kunitz胰蛋白酶抑制剂3(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选出了几种特征肽(SEQ ID NO:8 GIGTIISSPYR；SEQ ID NO:64 CPLTVVQSR；SEQ ID NO:65 NELDK；SEQ ID NO:66IGENK；SEQ ID NO:67 DAMDGWFR；SEQ ID 68 LVFCPQQAEDDK；SEQ ID NO:69 CGDIGISIDHDDGTR；SEQ ID NO:70 LVVSK；和SEQ ID NO:71 NKPLVVQFQK)，这些特征肽的代表性定量显示在5A-5I中。为SEQ ID NO:8 GIGTIISSPYR合成肽标准品用于定量和定性分析(参见图5J)。合成肽可以直接作为蛋白质定量的分析参考标准。

实施例6

从包括NCBI，Phytozome和UniProt在内的公共数据库鉴定了Gly m 8(2S白蛋白)的数个同源蛋白质序列。使用生物信息学工具分析鉴定出的序列(SEQ ID NO:25和72-74)，以鉴定可用的蛋白质序列之间的序列同源性和共有序列组成(参见图6J)。具体地，这涉及使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，对其进行计算机上消化以产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，选定的特征肽的定量不仅可以测量Gly m 8(2S白蛋白)本身，而且还可以测量与Gly m 8(2S白蛋白)高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于Gly m 8(2S白蛋白)(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选择了几个特征肽(SEQ ID NO:11 IMENQSEELEEK；SEQ ID NO:75 WQHQQDSCR；SEQ ID NO:76 QLQGVNLTPCEK；SEQ ID NO:77 HIMEK；SEQ ID NO:78 DEDEEEEGHMQK；SEQ ID NO:79 CCTEMSELR；SEQ ID NO:80 ELINLATMCR；和SEQ ID NO:81 FGPMIQCDLSSDD)，这些标志肽的代表性定量显示于6A-6H中。为SEQ ID NO:11 IMENQSEELEEK合成肽标准品用于定量和定性分析(参见图6I)。合成肽可以直接作为蛋白质定量的分析参考标准。

实施例7

从包括NCBI，Phytozome和UniProt在内的公共数据库鉴定出凝集素的数个同源蛋白质序列。使用生物信息学工具分析鉴定出的序列(SEQ ID NO:26和83-90)以鉴定可用蛋白序列之间的序列同源性和共有序列组成(参见图7F)。具体地，这涉及使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，对其进行计算机上消化以产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，选定的特征肽的定量不仅可以测量凝集素本身，而且还可以测量与凝集素高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于凝集素(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选择了几个特征肽(SEQ ID NO:91 VFSPNK；SEQ ID NO:92 ANSTNTVSFTVSK；SEQ ID NO:93 QQNLIFQGDAAISPSGVLR；和SEQ ID NO:94 TADGLAFFLAPVGSKPQSK)，并且这些特征肽的代表性定量显示在图7A-7D中。为SEQ ID NO:91 VFSPNK合成肽标准品用于定量和定性分析(参见图7E)。合成肽可以直接作为蛋白质定量的分析参考标准。

实施例8

从包括NCBI，Phytozome和UniProt在内的公共数据库鉴定出脂加氧酶的数个同源蛋白质序列。使用生物信息学工具分析鉴定出的序列(SEQ ID NO:27和96-104)以鉴定可用蛋白序列之间的序列同源性和共有序列组成(参见图8R)。具体地，这涉及使用Vector NTI Align X比对工具，该工具执行CLUSTAL W型比对。从该分析出发，可以确定共有序列和/或代表性序列。

一旦选择或测定了共有序列和/或代表性序列，对其进行计算机上消化以产生待通过LC-MS检测和测量的候选特征肽片段。根据本文提供的独特方法，基于可用的蛋白质序列之间的保守程度选择特征肽，使得所选出的特征肽可以用于在公共序列数据库中鉴定出的蛋白质序列中定量所有或尽可能多的蛋白质同种型。因此，选定的特征肽的定量不仅可以测量脂加氧酶本身，而且还可以测量与脂加氧酶高度同源的潜在变应原。

将大豆种子样品研磨成细粉，用己烷脱脂两次，并用合适的测定缓冲液(例如5M尿素，2M硫脲，50mM Tris(pH 8.0)，65mM DTT)提取。将样品在缓冲液中超声处理以提取蛋白质。将提取的蛋白质稀释，变性，然后通过加入胰蛋白酶蛋白酶并在37℃下孵育15-20小时进行蛋白水解消化。消化反应用甲酸(pH＝1-2)酸化，并使用LC-MS/MS分析。

所选出的特征肽可以用于脂加氧酶(单独或在多重测定型式中与其它蛋白质组合)的定性和定量分析。在该实施例中，从所有肽可能性中选择了几个特征肽(SEQ ID NO:105 SSDFLTYGIK；SEQ ID NO:106 GTVVLMPK；SEQ ID NO:107 NVLDFNAITSIGK；SEQ ID NO:108 GGVIDTATGILGQGVSLVGGVIDTATSFLGR；SEQ ID NO:109 IFFVNDTYLPSATPAPLLK；SEQ ID NO:110 DENFGHLK；SEQ ID NO:111 SLSHDVIPLFK；SEQ ID NO:112 SLYEGGIK；SEQ ID NO:113 TDGENVLQFPPPHVAK；SEQ ID NO:114 INSLPTAK；SEQ ID NO:115 TILFLK；SEQ ID NO:116 HLSVLHPIYK；SEQ ID NO:117 QSLINADGIIEK；SEQ ID NO:118 FIPAEGTPEYDEMVK；SEQ ID NO:119 ALEAFK；和SEQ ID NO:120 GIPNSISI)，并且这些特征肽的代表性定量显示在图8A-8P中。为SEQ ID NO:105 SSDFLTYGIK合成肽标准品用于定量和定性分析(参见图8Q)。合成肽可以直接作为蛋白质定量的分析参考标准。

序列表

SEQ ID NO:1 Gly m 1的示例性特征肽

SYPSNATCPR

SEQ ID NO:2 Gly m 3的示例性特征肽

YMVIQGEPGAVIR

SEQ ID NO:3 Gly m 5(β-伴大豆球蛋白)的示例性特征肽

NILEASYDTK

SEQ ID NO:4 Gly m 6(大豆球蛋白)G2的示例性特征肽

VTAPAMR

SEQ ID NO:5 Gly m 6(大豆球蛋白)G3的示例性特征肽

NNNPFSFLVPPK

SEQ ID NO:6 Gly m 6(大豆球蛋白)前体的示例性特征肽

ADFYNPK

SEQ ID NO:7 Kunitz胰蛋白酶抑制剂1的示例性特征肽

GGGIEVDSTGK

SEQ ID NO:8 Kunitz胰蛋白酶抑制剂3的示例性特征肽

GIGTIISSPYR

SEQ ID NO:9 Gly m Bd 28 K的示例性特征肽

NKPQFLAGAASLLR

SEQ ID NO:10 Gly m Bd 30 K的示例性特征肽

GVITQVK

SEQ ID NO:11 Gly m 8(2S白蛋白)的示例性特征肽

IMENQSEELEEK

SEQ ID NO:12 Gly m 1 ABA54898.1[MW＝12482.64Da]

MGSKVVASVALLLSINILFISMVSSSSHYDPQPQPSHVTALITRPSCPDLSICLNILGGSLGTVDDCCALIGGLGDIEAIVCLCIQLRALGILNLNRNLQLILNSCGRSYPSNATCPRT

SEQ ID NO:13 Gly m 3 CAA11755.1[MW＝14100.07Da]

MSWQAYVDDHLLCGIEGNHLTHAAIIGQDGSVWLQSTDFPQFKPEEITAIMNDFNEPGSLAPTGLYLGGTKYMVIQGEPGAVIRGKKGPGGVTVKKTGAALIIGIYDEPMTPGQCNMVVERLGDYLIDQGY

SEQ ID NO:14 Gly m 4P26987[MW＝16771.81Da]

MGVFTFEDEINSPVAPATLYKALVTDADNVIPKALDSFKSVENVEGNGGPGTIKKITFLEDGETKFVLHKIESIDEANLGYSYSVVGGAALPDTAEKITFDSKLVAGPNGGSAGKLTVKYETKGDAEPNQDELKTGKAKADALFKAIEAYLLAHPDYN

SEQ ID NO:15 Gly m 5(β-伴大豆球蛋白)121281[MW＝70293.13Da]MMRARFPLLLLGLVFLASVSVSFGIAYWEKENPKHNKCLQSCNSERDSYRNQACHARCNLLKVEKEECEEGEIPRPRPRPQHPEREPQQPGEKEEDEDEQPRPIPFPRPQPRQEEEHEQREEQEWPRKEEKRGEKGSEEEDEDEDEEQDERQFPFPRPPHQKEERNEEEDEDEEQQRESEESEDSELRRHKNKNPFLFGSNRFETLFKNQYGRIRVLQRFNQRSPQLQNLRDYRILEFNSKPNTLLLPNHADADYLIVILNGTAILSLVNNDDRDSYRLQSGDALRVPSGTTYYVVNPDNNENLRLITLAIPVNKPGRFESFFLSSTEAQQSYLQGFSRNILEASYDTKFEEINKVLFSREEGQQQGEQRLQESVIVEISKEQIRALSKRAKSSSRKTISSEDKPFNLRSRDPIYSNKLGKFFEITPEKNPQLRDLDIFLSIVDMNEGALLLPHFNSKAIVILVINEGDANIELVGLKEQQQEQQQEEQPLEVRKYRAELSEQDIFVIPAGYPVVVNATSNLNFFAIGINAENNQRNFLAGSQDNVISQIPSQVQELAFPGSAQAVEKLLKNQRESYFVDAQPKKKEEGNKGRKGPLSSILRAFY

SEQ ID NO:16 Gly m 6大豆球蛋白G1 121276[MW＝55706.34Da]

MAKLVFSLCFLLFSGCCFAFSSREQPQQNECQIQKLNALKPDNRIESEGGLIETWNPNNKPFQCAGVALSRCTLNRNALRRPSYTNGPQEIYIQQGKGIFGMIYPGCPSTFEEPQQPQQRGQSSRPQDRHQKIYNFREGDLIAVPTGVAWWMYNNEDTPVVAVSIIDTNSLENQLDQMPRRFYLAGNQEQEFLKYQQEQGGHQSQKGKHQQEEENEGGSILSGFTLEFLEHAFSVDKQIAKNLQGENEGEDKGAIVTVKGGLSVIKPPTDEQQQRPQEEEEEEEDEKPQCKGKDKHCQRPRGSQSKSRRNGIDETICTMRLRHNIGQTSSPDIYNPQAGSVTTATSLDFPALSWLRLSAEFGSLRKNAMFVPHYNLNANSIIYALNGRALIQVVNCNGERVFDGELQEGRVLIVPQNFVVAARSQSDNFEYVSFKTNDTPMIGTLAGANSLLNALPEEVIQHTFNLKSQQARQIKNNNPFKFLVPPQESQKRAVA

SEQ ID NO:17 Gly m 6大豆球蛋白G2 121277[MW＝54390.76Da]

MAKLVLSLCFLLFSGCFALREQAQQNECQIQKLNALKPDNRIESEGGFIETWNPNNKPFQCAGVALSRCTLNRNALRRPSYTNGPQEIYIQQGNGIFGMIFPGCPSTYQEPQESQQRGRSQRPQDRHQKVHRFREGDLIAVPTGVAWWMYNNEDTPVVAVSIIDTNSLENQLDQMPRRFYLAGNQEQEFLKYQQQQQGGSQSQKGKQQEEENEGSNILSGFAPEFLKEAFGVNMQIVRNLQGENEEEDSGAIVTVKGGLRVTAPAMRKPQQEEDDDDEEEQPQCVETDKGCQRQSKRSRNGIDETICTMRLRQNIGQNSSPDIYNPQAGSITTATSLDFPALWLLKLSAQYGSLRKNAMFVPHYTLNANSIIYALNGRALVQVVNCNGERVFDGELQEGGVLIVPQNFAVAAKSQSDNFEYVSFKTNDRPSIGNLAGANSLLNALPEEVIQHTFNLKSQQARQVKNNNPFSFLVPPQESQRRAVA

SEQ ID NO:18 Gly m 6大豆球蛋白G3 121278[MW＝54241.73Da]

MAKLVLSLCFLLFSGCCFAFSFREQPQQNECQIQRLNALKPDNRIESEGGFIETWNPNNKPFQCAGVALSRCTLNRNALRRPSYTNAPQEIYIQQGSGIFGMIFPGCPSTFEEPQQKGQSSRPQDRHQKIYHFREGDLIAVPTGFAYWMYNNEDTPVVAVSLIDTNSFQNQLDQMPRRFYLAGNQEQEFLQYQPQKQQGGTQSQKGKRQQEEENEGGSILSGFAPEFLEHAFVVDRQIVRKLQGENEEEEKGAIVTVKGGLSVISPPTEEQQQRPEEEEKPDCDEKDKHCQSQSRNGIDETICTMRLRHNIGQTSSPDIFNPQAGSITTATSLDFPALSWLKLSAQFGSLRKNAMFVPHYNLNANSIIYALNGRALVQVVNCNGERVFDGELQEGQVLIVPQNFAVAARSQSDNFEYVSFKTNDRPSIGNLAGANSLLNALPEEVIQQTFNLRRQQARQVKNNNPFSFLVPPKESQRRVVA

SEQ ID NO:19 Gly m 6大豆球蛋白G4 121279[MW＝63587.16Da]

MGKPFTLSLSSLCLLLLSSACFAISSSKLNECQLNNLNALEPDHRVESEGGLIQTWNSQHPELKCAGVTVSKLTLNRNGLHSPSYSPYPRMIIIAQGKGALGVAIPGCPETFEEPQEQSNRRGSRSQKQQLQDSHQKIRHFNEGDVLVIPPSVPYWTYNTGDEPVVAISLLDTSNFNNQLDQTPRVFYLAGNPDIEYPETMQQQQQQKSHGGRKQGQHQQEEEEEGGSVLSGFSKHFLAQSFNTNEDIAEKLESPDDERKQIVTVEGGLSVISPKWQEQQDEDEDEDEDDEDEQIPSHPPRRPSHGKREQDEDEDEDEDKPRPSRPSQGKRNKTGQDEDEDEDEDQPRKSREWRSKKTQPRRPRQEEPRERGCETRNGVEENICTLKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFQLSAQYVVLYKNGIYSPHWNLNANSVIYVTRGQGKVRVVNCQGNAVFDGELRRGQLLVVPQNFVVAEQAGEQGFEYIVFKTHHNAVTSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGSPRVKVA

SEQ ID NO:20 Gly m 6大豆球蛋白前体75221455[MW＝63876.47Da]MGKPFTLSLSSLCLLLLSSACFAISSSKLNECQLNNLNALEPDHRVEFEGGLIQTWNSQHPELKCAGVTVSKLTLNRNGLHLPSYSPYPRMIIIAQGKGALQCKPGCPETFEEPQEQSNRRGSRSQKQQLQDSHQKIRHFNEGDVLVIPPGVPYWTYNTGDEPVVAISLLDTSNFNNQLDQTPRVFYLAGNPDIEYPETMQQQQQQKSHGGRKQGQHQQEEEEEGGSVLSGFSKHFLAQSFNTNEDIAEKLQSPDDERKQIVTVEGGLSVISPKWQEQQDEDEDEDEDDEDEQIPSHPPRRPSHGKREQDEDEDEDEDKPRPSRPSQGKREQDQDQDEDEDEDEDQPRKSREWRSKKTQPRRPRQEEPRERGCETRNGVEENICTLKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFQLSAQYVVLYKNGIYSPHWNLNANSVIYVTRGQGKVRVVNCQGNAVFDGELRRGQLLVVPQNFVVAEQAGEQGFEYIVFKTHHNAVTSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGSPRVKVA

SEQ ID NO:21 Gly m Bd 28K 12697782[MW＝52944.36Da]

MGNKTTLLLLLFVLCHGVATTTMAFHDDEGGDKKSPKSLFLMSNSTRVFKTDAGEMRVLKSHGGRIFYRHMHIGFISMEPKSLFVPQYLDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFYIGGGANSHSVLSGFEPAILETAFNESRTVVEEIFSKELDGPIMFVDDSHAPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGYPNGSRAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASLLRTLMGPELSAAFGVSEDTLRRAVDAQHEAVILPSAWAAPPENAGKLKMEEEPNAIRSFANDVVMDVF

SEQ ID NO:22 Gly m Bd 30K 84371705[MW＝42757.81Da]

MGFLVLLLFSLLGLSSSSSISTHRSILDLDLTKFTTQKQVSSLFQLWKSEHGRVYHNHEEEAKRLEIFKNNLNYIRDMNANRKSPHSHRLGLNKFADITPQEFSKKYLQAPKDVSQQIKMANKKMKKEQYSCDHPPASWDWRKKGVITQVKYQGGCGSGWAFSATGAIEAAHAIATGDLVSLSEQELVDCVEESEGCYNGWHYQSFEWVLEHGGIATDDDYPYRAKEGRCKANKIQDKVTIDGYETLIMSDESTESETEQAFLSAILEQPISVSIDAKDFHLYTGGIYDGENCTSPYGINHFVLLVGYGSADGVDYWIAKNSWGEDWGEDGYIWIQRNTGNLLGVCGMNYFASYPTKEESETLVSARVKGHRRVDHSPL

SEQ ID NO:23 KTI 1 125722[MW＝22545.94Da]

MKSTIFFALFLVCAFTISYLPSATAQFVLDTDDDPLQNGGTYYMLPVMRGKGGGIEVDSTGKEICPLTVVQSPNELDKGIGLVFTSPLHALFIAERYPLSIKFGSFAVITLCAGMPTEWAIVEREGLQAVKLAARDTVDGWFNIERVSREYNDYKLVFCPQQAEDNKCEDIGIQIDDDGIRRLVLSKNKPLVVQFQKFRSSTA

SEQ ID NO:24 KTI 3 125020[MW＝24005.29Da]

MKSTIFFLFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGGIRAAPTGNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPTEWSVVEDLPEGPAVKIGENKDAMDGWFRLERVSDDEFNNYKLVFCPQQAEDDKCGDIGISIDHDDGTRRLVVSKNKPLVVQFQKLDKESLAKKNHGLSRSE

SEQ ID NO:25 Gly m 8(2S白蛋白)NP_001238443[MW＝18459.97Da]MTKFTILLISLLFCIAHTCSASKWQHQQDSCRKQLQGVNLTPCEKHIMEKIQGRGDDDDDDDDDNHILRTMRGRINYIRRNEGKDEDEEEEGHMQKCCTEMSELRSPKCQCKALQKIMENQSEELEEKQKKKMEKELINLATMCRFGPMIQCDLSSDD

SEQ ID NO:26 凝集素ADC94422[MW＝30186.22Da]

MATSNFSIVLSLSLAFFLVLLTKANSTNTVSFTVSKFSPRQQNLIFQGDAAISPSGVLRLTKVDSIDVPTTGSLGRALYATPIQIWDSETGKVASWATSFKFKVFSPNKTADGLAFFLAPVGSKPQSKGGFLGLFNSDSKNKSVQTVAVEFDTYYNAKWDPANRHIGIDVNSIKSVKTASWGLANGQIAQILITYDADTSLLVASLIHPSRKTSYILSETVSLKSNLPEWVNIGFSATTGLNKGFVETHDVFSWSFASKLSDGSTSDTLDLPSFLLNEAI

SEQ ID NO:27脂氧合酶CAA39604[MW＝96817.14Da]

MFGIFDKGQKIKGTVVLMPKNVLDFNAITSIGKGGVIDTATGILGQGVSLVGGVIDTATSFLGRNISMQLISATQTDGSGNGKVGKEVYLEKHLPTLPTLGARQDAFSIFFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTIEFVCNSWVYNFRSYKKNRIFFVNDTYLPSATPAPLLKYRKEELEVLRGDGTGKRKDFDRIYDYDVYNDLGNPDGGDPRPILGGSSIYPYPRRVRTGRERTRTDPNSEKPGEVYVPRDENFGHLKSSDFLTYGIKSLSHDVIPLFKSAIFQLRVTSSEFESFEDVRSLYEGGIKLPTDILSQISPLPALKEIFRTDGENVLQFPPPHVAKVSKSGWMTDEEFAREVIAGVNPNVIRRLQEFPPKSTLDPTLYGDQTSTITKEQLEINMGGVTVEEALSTQRLFILDYQDAFIPYLTRINSLPTAKAYATRTILFLKDDGTLKPLAIELSKPHPDGDNLGPESIVVLPATEGVDSTIWLLAKAHVIVNDSGYHQLVSHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYRDTININGLARQSLINADGIIEKSFLPGKYSIEMSSSVYKNWVFTDQALPADLVKRGLAIEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSLYYPTDAAVQQDTELQAWWKEAVEKGHGDLKEKPWWPKMQTTEDLIQSCSIIVWTASALHAAVNFGQYPYGGLILNRPTLARRFIPAEGTPEYDEMVKNPQKAYLRTITPKFETLIDLSVIEILSRHASDEIYLGERETPNWTTDKKALEAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI

SEQ ID NO:28 Gly m Bd 28 K共有序列

VLCHGVATTTMAFHDDEGGDKKSPKSLFLMSNSTRVFKTDAGEMRVLKSHGGRIFYRHMHIGFISMEPKSLFVPQYLDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFYIGGGANSHSVLSGFEPAILETAFNESRTVVEEIFSKELDGPIMFVDDSHAPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGYPNGSKAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASLLRTLMGPELSAAFGVSEDTLRRAVDAQHEAVILPSAWAAPPENAGKLKMEEEP

SEQ ID NO:29 Gly m Bd 28 K Eric BAB21619 473aa

KTTLLLLLFVLCHGVATTTMAFHDDEGGDKKSPKSLFLMSNSTRVFKTDAGEMRVLKSHGGRIFYRHMHIGFISMEPKSLFVPQYLDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFYIGGGANSHSVLSGFEPAILETAFNESRTVVEEIFSKELDGPIMFVDDSHAPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGYPNGSRAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASLLRTLMGPELSAAFGVSEDTLRRAVDAQHEAVILPSAWAAPPENAGKLKMEEEPNAIRSFANDVVMDVF

SEQ ID NO:30 Gly m Bd 28 K Ping ACD36978.1 455aa

VLCHGVATTTMAFHDDEGGDKKSPKSLFLMSNSTRVFKTDAGEMRVLKSHGGRIFYRHMHIGFISMEPKSLFVPQYLDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFYIGGGANSHSVLSGFEPAILETAFNESRTVVEEIFSKELDGPIMFVDDSHAPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGPNGSKAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASLLRTLMGPELSAAFGVSEDTLRRAVDAQHEAVILPSAWAAPRKMQEAEMEESQMLLKLCQ

SEQ ID NO:31 Gly m Bd 28 K ACD36975.1 373aa

LDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFYIGGGANSHSVLSGFEPAILETAFNESRTVVEEIFSKELDGPIMFVDDSHAPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGYPNGSKAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASLLRTLMGPELSAAFGVSEDTLRRAVDAQHEAVILPSAWAAPPENAGKLKMEEEP

SEQ ID NO:32 Gly m Bd 28 K Ping ACD36976.1 373aa

LDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFNIGGGANSHSVLSGFEPAILETAFNESRTVVEETFSKELDGPIMFVDDSHAPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGYPNGSKAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASLLRTLMGPELSAAFGVSEDTLRRAVDAQHAAVILPSAWAAPPENAGKLKMEEEP

SEQ ID NO:33 Gly m Bd 28 K Ping ACD36974.1 320aa

LDSNLIIFIRRGEAKLGFIYDDELAERRLKTGDLYMIPSGSAFYLVNIGEGQRLHVICSIDPSTSLGLETFQSFYIGGGANSHSVLSGFEPAILETAFNESRTVVEEIFSKELDGPIMFVDDSHVPSLWTKFLQLKKDDKEQQLKKMMQDQEEDEEEKQTSRSWRKLLETVFGKVNEKIENKDTAGSPASYNLYDDKKADFKNAYGWSKALHGGEYPPLSEPDIGVLLVKLSAGSMLAPHVNPISDEYTIVLSGYGELHIGYPNGSKAMKTKIKQGDVFVVPRYFPFCQVASRDGPLEFFGFSTSARKNKPQFLAGAASL

SEQ ID NO:34 LGFIYDDELAER

SEQ ID NO:35 TVVEEIFSK

SEQ ID NO:36 MMQDQEEDEEEK

SEQ ID NO:37 NAYGWSK

SEQ ID NO:38 ALHGGEYPPLSEPDIGVLLVK

SEQ ID NO:39 QGDVFVVPR

SEQ ID NO:40 YFPFCQVASR

SEQ ID NO:41 TLMGPELSAAFGVSEDTLR

SEQ ID NO:42 SFANDVVMDVF

SEQ ID NO:43 Gly m Bd 30 K Ping AAB09252.1 379aa(也充当共有序列)MGFLVLLLFSLLGLSSSSSISTHRSILDLDLTKFTTQKQVSSLFQLWKSEHGRVYHNHEEEAKRLEIFKNNSNYIRDMNANRKSPHSHRLGLNKFADITPQEFSKKYLQAPKDVSQQIKMANKKMKKEQYSCDHPPASWDWRKKGVITQVKYQGGCGRGWAFSATGAIEAAHAIATGDLVSLSEQELVDCVEESEGSYNGWQYQSFEWVLEHGGIATDDDYPYRAKEGRCKANKIQDKVTIDGYETLIMSDESTESETEQAFLSAILEQPISVSIDAKDFHLYTGGIYDGENCTSPYGINHFVLLVGYGSADGVDYWIAKNSWGEDWGEDGYIWIQRNTGNLLGVCGMNYFASYPTKEESETLVSARVKGHRRVDHSPL

SEQ ID NO:44 Gly m Bd 30 K Ping P22895.1 379aa

MGFLVLLLFSLLGLSSSSSISTHRSILDLDLTKFTTQKQVSSLFQLWKSEHGRVYHNHEEEAKRLEIFKNNSNYIRDMNANRKSPHSHRLGLNKFADITPQEFSKKYLQAPKDVSQQIKMANKKMKKEQYSCDHPPASWDWRKKGVITQVKYQGGCGRGWAFSATGAIEAAHAIATGDLVSLSEQELVDCVEESEGSYNGWQYQSFEWVLEHGGIATDDDYPYRAKEGRCKANKIQDKVTIDGYETLIMSDESTESETEQAFLSAILEQPISVSIDAKDFHLYTGGIYDGENCTSPYGINHFVLLVGYGSADGVDYWIAKNSWGFDWGEDGYIWIQRNTGNLLGVCGMNYFASYPTKEESETLVSARVKGHRRVDHSPL

SEQ ID NO:45 SILDLDLTK

SEQ ID NO:46 FTTQK

SEQ ID NO:47 NNLNYIR

SEQ ID NO:48 FADITPQEFSK

SEQ ID NO:49 EQYSCDHPPASWDWR

SEQ ID NO:50 VTIDGYETLIMSDESTESETEQAFLSAILEQPISVSIDAK

SEQ ID NO:51 NTGNLLGVCGMNYFASYPTK

SEQ ID NO:52 KTI 1共有序列

MKSTIFFALFLVCAFTISYLPSATAQFVLDTDDDPLQNGGTYYMLPVMRGKGGGIEGASTGKEICPLTVVQSPNELDKGIGLVFSSPLHALFIAERYPLSIKFGSFAVISLCGGMPTKWAIVEREGLQAVTLAARDTVDGWFNIERVSREYNDYKLVFCPQNAEDNKCEDIGIQIDNDGIRRLVLSKNKPLVVQFQKFRSSTA

SEQ ID NO:53 KTI 1 AAB23483.1 204aa

MKSTIFFALFLVCAFTISYLPSATAQFVLDTDDDPLQNGGTYYMLPVMRGKSGGIEGNSTGKEICPLTVVQSPNKHNKGIGLVFKSPLHALFIAERYPLSIKFDSFAVIPLCGVMPTKWAIVEREGLQAVTLAARDTVDGWFNIERVSREYNDYYKLVFCPQEAEDNKCEDIGIQIDNDGIRRLVLSKNKPLVVEFQKFRSSTA

SEQ ID NO:54 KTI 1 CAA56343.1 208aa

MKSTTSLALFLLCALTSSYQPSATADIVFDTEGNPIRNGGTYYVLPVIRGKGGGIEFAKTETETCPLTVVQSPFEGLQRGLPLIISSPFKILDITEGLILSLKFHLCTPLSLNSFSVDRYSQGSARRTPCQTHWLQKHNRCWFRIQRASSESNYYKLVFCTSNDDSSCGDIVAPIDREGNRPLIVTHDQNHPLLVQFQKVEAYESSTA

SEQ ID NO:55 EICPLTVVQSPNELDK

SEQ ID NO:56 EGLQAVK

SEQ ID NO:57 LVFCPQQAEDNK

SEQ ID NO:58 CEDIGIQIDDDGIR

SEQ ID NO:59 LVLSK

SEQ ID NO:60 NKPLVVQFQK

SEQ ID NO:61 KTI 3共有序列

MKSTIFFALFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGGIRAAPTGNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPTEWSVVEDLPEGPAVKIGENKDAMDGWFRLERVSDDEFNNYKLVFCPQQAEDDKCGDIGISIDHDDGTRRLVVSKNKPLVVQFQKLDKESLAKKNHGLSRSE

SEQ ID NO:62 KTI 3 CAA45777.1 217aa

MKSTIFFALFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGGIRAAPTGNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPTEWSVVEDLPEGPAVKIGENKDAMDGWFRLERVSDDEFNNYKLVFCPQQAEDDKCGDIGISIDHDDGTRRLVVSKNKPLVVQFQKLDKESLAKKNHGLSRSE

SEQ ID NO:63 KTI 3 CAA45778.1 217aa

MKSTIFFALFLFCAFTTSYLPSAIADFVLDNEGNPLDSGGTYYILSDITAFGGIRAAPTGNERCPLTVVQSRNELDKGIGTIISSPFRIRFIAEGNPLRLKFDSFAVIMLCVGIPTEWSVVEDLPEGPAVKIGENKDAVDGWFRIERVSDDEFNNYKLVFCTQQAEDDKCGDIGISIDHDDGTRRLVVSKNKPLVVQFQKVDKESLAKKNHGLSRSE

SEQ ID NO:64 CPLTVVQSR

SEQ ID NO:65 NELDK

SEQ ID NO:66 IGENK

SEQ ID NO:67 DAMDGWFR

SEQ ID NO:68 LVFCPQQAEDDK

SEQ ID NO:69 CGDIGISIDHDDGTR

SEQ ID NO:70 LVVSK

SEQ ID NO:71 NKPLVVQFQK

SEQ ID NO:72 2S白蛋白Glyma13g36400.1 BLAST 174aa

MPPPSLHFTSPINSKMTKFTILLISLLFCIAHTCSASKWQHQQDSCRKQLQGVNLTPCEKHIMEKIQGRGDDDDDDDDDNHILRTMRGRINYIRRNEGKDEDEEEEGHMQKCCTEMSELRSPKCQCKALQKIMENQSEELEEKQKKKMEKELINLATMCRFGPMIQCDLSSDD

SEQ ID NO:73 2S白蛋白NP_001234950 BLAST 158aa

MTKFTILLISLLFCIAHTCSASEWQHQQDSCRKQLQGVNLTPCEKHIMEKIQGRGDDDDDDDDDNHILRTMRGRINYIRRNEGKDEDEEEEGHMQKCRTEMSELRSPKCQCKALQKIMENQSEELEEKQKKKMEKELINLATMCRFGPMIQCDLSSDD

SEQ ID NO:74 2S白蛋白Glyma12g34160.1 BLAST 156aa

MTKLTILLIALLFIAHTCCASKWQQHQQESCREQLKGINLNPCEHIMEKIQAGRRGEDGSDEDHILIRTMPGRINYIRKKEGKEEEEEGHMQKCCSEMSELKSPICQCKALQKIMDNQSEQLEGKEKKQMERELMNLAIRCRLGPMIGCDLSSDD

SEQ ID NO:75 WQHQQDSCR

SEQ ID NO:76 QLQGVNLTPCEK

SEQ ID NO:77 HIMEK

SEQ ID NO:78 DEDEEEEGHMQK

SEQ ID NO:79 CCTEMSELR

SEQ ID NO:80 ELINLATMCR

SEQ ID NO:81 FGPMIQCDLSSDD

SEQ ID NO:82 凝集素共有序列

MATSNFSIVLSLSLAFFLVLLTKANSTNTVSFTFSKFSPRQPNLILQGDAAISSSGVLRLTKVDSNGVPTSGSLGRALYAAPIQIWDSETGKVASWATSFKFNVFAPNKTADGLAFFLAPVGSKPQSKGGFLGLFNSDSKDKSLQTVAVEFDTYSNKKWDPANRHIGIDVNSIKSVKTASWGLANGQVAQILITYDAATSLLVASLIHPSRKTSYILSETVSLKSNLPEWVSIGFSATTGLNEGSVETHDVISWSFASKLSDGSTSDALDLPSFLLNEAI

SEQ ID NO:83 凝集素XP_003535884BLAST 280aa

MATSNFSIVLSLSLALFLMLLTKANSTNTVSFTTSKFSPRQQNLILQGDAAISPSGVLRLTKVDSYGVPTSRSLGRALYAAPIQIWDSETGKVASWATSFKFNVFSPDKTADGLAFFLAPVGSKPQYKAGFLGLFNSDSKNMSLQTVAVEFDTYYNQKWDPASRHIGIDVNSIKSVKTAPWGFANGQVAQILITYNADTSLLVASLVHPSRKTSYILSETVSLKSNLPEWVNVGFSATTGANKGFAETHDVFSWSFASKLSDGSTSDTLDLASFLLNEAI

SEQ ID NO:84 凝集素Glyma10g15480.1 BLAST

MATSNFSIVLSLSLALFLMLLTKANSTNTVSFTTSKFSPRQQNLILQGDAAISPSGVLRLTKVDSYGVPTSRSLGRALYAAPIQIWDSETGKVASWATSFKFNVFSPDKTADGLAFFLAPVGSKPQYKAGFLGLFNSDSKNMSLQTVAWDPASRHIGIDVNSIKSVKTAPWGFANGQVAQILITYNADTSLLVASLVHPSRKTSYILSETVSLKSNLPEWVNVGFSATTGANKGFAETHDVFSWSFASKLSDGSTSDTLDLASFLLNEAI

SEQ ID NO:85 凝集素NP_001237210 BLAST 282aa

MATSNFSIVLSVSLAFFLVLLTKAHSTDTVSFTFNKFNPVQPNIMLQKDASISSSGVLQLTKVGSNGVPTSGSLGRALYAAPIQIWDSETGKVASWATSFKFNIFAPNKSNSADGLAFFLAPVGSQPQSDDGFLGLFNSPLKDKSLQTVAIEFDTFSNKKWDPANRHIGIDVNSIKSVKTASWGLSNGQVAEILVTYNAATSLLVASLIHPSKKTSYILSDTVNLKSNLPEWVSVGFSATTGLHEGSVETHDVISWSFASKLSDGSSNDALDLPSFVLNEAI

SEQ ID NO:86 凝集素Glyma02g18090.1 BLAST

MKVLCIIFEFKQIKAMATSNFSIVLSVSLAFFLVLLTKAHSTDTVSFTFNKFNPVQPNIMLQKDASISSSGVLQLTKVGSNGVPTSGSLGRALYAAPIQIWDSETGKVASWATSFKFNIFAPNKSNSADGLAFFLAPVGSQPQSDDGFLGLFNSPLKDKSLQTVAIEFDTFSNKKWDPANRHIGIDVNSIKSVKTASWGLSNGQVAEILVTYNAATSLLVASLIHPSKKTSYILSDTVNLKSNLPEWVSVGFSATTGLHEGSVETHDVISWSFASKLSDGSSNDALDLPSFVLNEAI

SEQ ID NO:87 凝集素ACU23599BLAST 282aa

MATSNFSIVLSVSLAFFLVLLTKAHPTDTVSFTFNKFNPVQPNIMLQKDASISSSGVLQLTKVGSNGVPTSGSLGRALYAAPIQIWDSETGKVASWATSFKFNIFAPNKSNSADGLAFFLAPVGSQPQSDDGFLGLFNSPLKDKSLQTVAIEFDTFSNKKWDPANRHIGIDVDSIKSIKTASWGLSNGQVAEILVTYNAATSLLVASLIHPSKKTSYILSDTVNLKSNLPEWVSVGFSATTGLHEGSVETHDVISWSFASKLSDGSSNDALDLPSFVLNEAI

SEQ ID NO:88 凝集素CAH60173BLAST 280aa

MASSKFSTVISFSLALFLVLLTQANSTNIFSFNFQTFDSPNLIFQGDASVSSSGQLRLTKVKGNGKPTAASLGRAFYSAPIQIWDSTTGNVASFATSFTFNILAPNKSNSADGLAFALVPVGSQPKSNGGFLGLFDNATYDSSAQTVAVEFDTYSNPKWDPENRHIGIDVNSIESIRTASWGLANGQNAEILITYDSSTKLLVASLVHPSRRTSYIVSERVDLKSVLPEWVSIGFSATTGLLEGSIETHDVLSWSFASKLSDDTTSEGLNLANFVLNKIL

SEQ ID NO:89 凝集素Glyma10g01620.2 BLAST 228aa

MATSKFHTQKPLFVVLSVVVVLLTMTKVNSTKPFLSPGTSSCRTNRTLILQGDALVTSSRKSLGRALYSTPIHIWDSEIGSVASFAASFNFTVYASDIANLADGLAFFLAPIDTQPQTRGGYLGLYNNPSNSSWGLANDQVTNVLITYDASTNLLVASLVHPSQRSSYILSDVLDLKVALPEWVRIGFSATTGLNVASETHDVHSWSFSSNLPFGSSNTNPSDFAIFI

SEQ ID NO:90 凝集素Glyma02g01590.1 BLAST 285aa

MATSKLKTQNVVVSLSLTLTLVLVLLTSKANSAETVSFSWNKFVPKQPNMILQGDAIVTSSGKLQLNKVDENGTPKPSSLGRALYSTPIHIWDKETGSVASFAASFNFTFYAPDTKRLADGLAFFLAPIDTKPQTHAGYLGLFNENESGDQVVAVEFDTFRNSWDPPNPHIGINVNSIRSIKTTSWDLANNKVAKVLITYDASTSLLVASLVYPSQRTSNILSDVVDLKTSLPEWVRIGFSAATGLDIPGESHDVLSWSFASNLPHASSNIDPLDLTSFVLHEAI

SEQ ID NO:91 VFSPNK

SEQ ID NO:92 ANSTNTVSFTVSK

SEQ ID NO:93 QQNLIFQGDAAISPSGVLR

SEQ ID NO:94 TADGLAFFLAPVGSKPQSK

SEQ ID NO:95脂加氧酶共有序列

MGGIFDKGQKIKGTVVLMPKNVLDFNAITSIGKGGVIDTATGILGAGVSLVGGVIDTATAFLGRNISMQLISATQTDGSGNGKVGKEVYLEKHLPTLPTLGARQDAFSIFFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTIEFVCNSWVYNFKSYKKNRIFFVNDTYLPSATPAPLLKYRKEELEVLRGDGTGKRKDFDRIYDYDVYNDLGNPDGGDPRPILGGSSIYPYPRRVRTGRERTRTDPNSEKPGEVYVPRDENFGHLKSSDFLTYGIKSLSHDVIPLFKSAIFQLRVTSSEFDSFEDVRSLYEGGIKLPTDILSQISPLPALKEIFRTDGENVLQFPPPHVAKVSKSGWMTDEEFAREMIAGVNPNVIRRLQEFPPKSTLDPTLYGDQTSTITKEQLEINMGGVTVEEALSTQRLFILDYQDAFIPYLTRINSLPTAKAYATRTILFLKDDGTLKPLAIELSKPHPDGDNLGPESIVVLPATEGVDSTIWLLAKAHVIVNDSGYHQLVSHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYRDTININGLARQSLINADGIIEKSFLPGKYSIEMSSSVYKNWVFTDQALPADLVKRGLAIEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSLYYPTDAAVQQDTELQAWWKEAVEKGHGDLKDKPWWPKMQTTEDLIQSCSIIIWTASALHAAVNFGQYPYGGLILNRPTLARRFIPEEGTPEYDEMVKNPQKAYLRTITPKFETLIDLSVIEILSRHASDEIYLGERDTPNWTTDKKALEAFKKFGSKLTGIEGKINARNSDPSLRNRTGPV QLPYTLLHRSSEEGLTFKGIPNSISI

SEQ ID NO:96脂加氧酶2IUK_A BLAST 864aa

MFGIFDKGQKIKGTVVLMPKNVLDFNAITSIGKGGVIDTATGILGQGVSLVGGVIDTATSFLGRNISMQLISATQTDGSGNGKVGKEVYLEKHLPTLPTLGARQDAFSIFFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTIEFVCNSWVYNFRSYKKNRIFFVNDTYLPSATPAPLLKYRKEEFEVLRGDGTGKRKDFDRIYDYDVYNDLGNPDGGDPRPILGGCSIYPYPLRVRTGRERTRTDPNSEKPGEVYVPRDENFGHLKSSDFLTYGIKSLSHDVIPLFKSAIFQLRVTSSEFESFEDVRSLYEGGIKLPTDILSQISPLPALKEIFRTDGENVLQFPPPHVAKVSKSGVMTDEEFAREVIAGVNPNVIRRLQEFPPKSTLDPTLYGDQTSTITKEQLEINMGGVTVEEALSTQRLFILDYQDAFIPYLTRINSLPTAKAYATRTILFLKDDGTLKPLAIELSKPHPDGDNLGPESIVVLPATEGVDSTIWLLAKAHVIVNDSGYHQLVSHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYRDTININGLARQSLINADGIIEKSFLPGKYSIEMSSSVYKNWVFTHQALPADLVKRGLAIEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSLYYPTDAAVQQDTELQAWWKEAVEKGHGDLKEKPWWPKKQTTEDLIQSCSIIVWTASALHAAVNFGQYPYGGLILNRPTLARRFIPAEGTPEYDEMVKNPQKAYLRTITPKFETLIDLSVIEILSRHASDEIYLGERETPNWTTDKKALEAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI

SEQ ID NO:97脂加氧酶NP_001238676 BLAST 864aa

MFGIFDKGQKIKGTVVLMPKNVLDFNAITSIGKGGVIDTATGILGQGVSLVGGVIDTATSFLGRNISMQLISATQTDGSGNGKVGKEVYLEKHLPTLPTLGARQDAFSIFFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTIEFVCNSWVYNFRSYKKNRIFFVNDTYLPSATPAPLLKYRKEELEVLRGDGTGKRKDFDRIYDYDVYNDLGNPDGGDPRPILGGCSIYPYPLRVRTGRERTRTDPNSEKPGEVYVPRDENFGHLKSSDFLTYGIKSLSHDVIPLFKSAIFQLRVTSSEFESFEDVRSLYEGGIKLPTDILSQISPLPALKEIFRTDGENVLQFPPPHVAKVSKSGWMTDEEFAREVIAGVNPNVIRRLQEFPPKSTLDPTLYGDQTSTITKEQLEINMGGVTVEEALSTQRLFILDYQDAFIPYLTRINSLPTAKAYATRTILFLKDDGTLKPLAIELSKPHPDGDNLGPESIVVLPATEGVDSTIWLLAKAHVIVNDSGYHQLVSHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYRDTININGLARQSLINADGIIEKSFLPGKYSIEMSSSVYKNWVFTHQALPADLVKRGLAIEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSLYYPTDAAVQQDTELQAWWKEAVEKGHGDLKEKPWWPKKQTTEDLIQSCSIIVWTASALHAAVNFGQYPYGGLILNRPTLARRFIPAEGTPEYDEMVKNPQKAYLRTITPKFETLIDLSVIEILSRHASDEIYLGERETPNWTTDKKALEAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI

SEQ ID NO:98脂加氧酶Glyma08g20220.1 BLAST 867aa

MLGLFDKSHKIKGTVVLMPKSVLDINDLNSVKNGGVGGVVSGIFGAVADVTGQIVDTATAIFSRNVSFKLISATSTDAKGNGKVGNETFLEKHLPTLPTLGDRRDAYDIHFEWDANFGIPGAFYIRNYTYDEFFLVSVTLEDIPNHGTIHFVCNSWVYNFKDYDKKDRIFFANKTYLPSATPGPLVKYREEELKILRGDGTGERKEHERIYDYDVYNDLGNPDEDVKLARPVLGGSSTYPYPRRVRTGRKATKKDPKSERPASELYMPRDEKFGHLKSSDFLTYGIKSLSQKLLPSLENVFDSDLTWNEFDSFEEVRDLYEGGIKVPTGVLSDISPIPIFKEIFRTDGESVLQFPPPHVVQVTKSAWMTDDEFAREMIAGVNPNVIRLLKEFPPQSKLDPSLYGDQSSTITKEHLEINMDGVTVEEALNGQRLFILDYQDAFMPYLTRINALPSAKAYATRTILLLKDDGTLKPLAIELSKPHPSGDNLGAESKVVLPADQGVESTIWLLAKAHVIVNDSGYHQLMSHWLNTHAVTEPFIIATNRRLSVLHPIYKLLYPHYRDTININGLARNALINAGGVIEESFLPGRYSIEMSSAVYKNWVFTDQALPVDLIKRGMAVEDPSSPHGLRLAVEDYPYAVDGLEIWDAIKSWVQEYVSLYYPTDLAIQQDTELQAWWKEVVEKGHGDLKDKPWWPKMQTRQELIQSCSTIIWIASALHAAVNFGQYPYGGFILNRPTLSRRWIPEPGTKEYDEMVESPQTAYLRTITPKRQTIIDLTVIEILSRHASDEIYLGERDNPNWTSDSKALEAFKKFGSKLAEIEGKITARNKDSNKKNRYGPVQLPYTLLLPTSEEGLTFRGIPNSISI

SEQ ID NO:99脂加氧酶P24095 BLAST 864aa

MFGIFDKGQKIKGTVVLMPKNVLDFNAITSIGKGGVIDTATGILGQGVSLVGGVIDTATSFLGRNISMQLISATQTDGSGNGKVGKEVYLEKHLPTLPTLGARQDAFSIFFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTIEFVCNSWVYNFRSYKKNRIFFVNDTYLPSATPAPLLKYRKEELEVLRGDGTGKRKDFDRIYDYDVYNDLGNPDGGDPRPILGGSSIYPYPRRVRTGRERTRTDPNSEKPGEVYVPRDENFGHLKSSDFLTYGIKSLSHDVIPLFKSAIFQLRVTSSEFESFEDVRSLYEGGIKLPTDILSQISPLPALKEIFRTDGENVLQFPPPHVAKVSKSGWMTDEEFAREVIAGVNPNVIRRLQEFPPKSTLDPTLYGDQTSTITKEQLEINMGGVTVEEALSTQRLFILDYQDAFIPYLTRINSLPTAKAYATRTILFLKDDGTLKPLAIELSKPHPDGDNLGPESIVVLPATEGVDSTIWLLAKAHVIVNDSGYHQLVSHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYRDTININGLARQSLINADGIIEKSFLPGKYSIEMSSSVYKNWVFTDQALPADLVKRGLAIEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSLYYPTDAAVQQDTELQAWWKEAVEKGHGDLKEKPWWPKMQTTEDLIQSCSIIVWTASALHAAVNFGQYPYGGLILNRPTLARRFIPAEGTPEYDEMVKNPQKAYLRTITPKFETLIDLSVIEILSRHASDEIYLGERETPNWTTDKKALEAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI

SEQ ID NO:100脂加氧酶Glyma07g00900.1 BLAST 866aa

MTGGMFGRKGQKIKGTVVLMPKNVLDFNAITSVGKGSAKDTATDFLGKGLDALGHAVDALTAFAGHSISLQLISATQTDGSGKGKVGNEAYLEKHLPTLPTLGARQEAFDINFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTINFVCNSWVYNFKSYKKNRIFFVNDTYLPSATPAPLLKYRKEELEVLRGDGTGKRKDFDRIYDYDVYNDLGNPDGGDPRPILGGSSIYPYPRRVRTGRERTRTDPNSEKPGEVYVPRDENFGHLKSSDFLTYGIKSLSHDVIPLFKSAIFQLRVTSSEFESFEDVRSLYEGGIKLPTDILSQISPLPALKEIFRTDGENVLQFPPPHVAKVSKSGWMTDEEFAREVIAGVNPNVIRRLQEFPPKSTLDPTLYGDQTSTITKEQLEINMGGVTVEEALSTQRLFILDYQDAFIPYLTRINSLPTAKAYATRTILFLKDDGTLKPLAIELSKPHPDGDNLGPESIVVLPATEGVDSTIWLLAKAHVIVNDSGYHQLVSHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYRDTININGLARQSLINADGIIEKSFLPGKYSIEMSSSVYKNWVFTDQALPADLVKRGLAIEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSLYYPTDAAVQQDTELQAWWKEAVEKGHGDLKEKPWWPKMQTTEDLIQSCSIIVWTASALHAAVNFGQYPYGGLILNRPTLARRFIPAEGTPEYDEMVKNPQKAYLRTITPKFETLIDLSVIEILSRHASDEIYLGERETPNWTTDKKALEAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI

SEQ ID NO:101脂加氧酶NP_001235189 BLAST 859aa

MTGGMFGRKGQKIKGTVVLMPKNVLDFNAITSVGKGSAKDTATDFLGKGLDALGHAVDALTAFAGHSISLQLISATQTDGSGKGKVGNEAYLEKHLPTLPTLGARQEAFDINFEWDASFGIPGAFYIKNFMTDEFFLVSVKLEDIPNHGTINFVCNSWVYNFKSYKKNRIFFVNDTYLPSATPGPLVKYRQEELEVLRGDGTGKRRDFDRIYDYDIYNDLGNPDGGDPRPIIGGSSNYPYPRRVRTGREKTRKDPNSEKPGEIYVPRDENFGHLKSSDFLTYGIKSLSQNVIPLFKSIILNLRVTSSEFDSFDEVRGLFEGGIKLPTNILSQISPLPVLKEIFRTDGENTLQFPPPHVIRVSKSGWMTDDEFAREMIAGVNPNVIRRLQEFPPKSTLDPATYGDQTSTITKQQLEINLGGVTVEEAISAHRLFILDYHDAFFPYLTKINSLPIAKAYATRTILFLKDDGSLKPLAIELSKPATVSKVVLPATEGVESTIWLLAKAHVIVNDSGYHQLISHWLNTHAVMEPFAIATNRHLSVLHPIYKLLYPHYKDTININGLARQSLINAGGIIEQTFLPGKYSIEMSSVVYKNWVFTDQALPADLVKRGLAVEDPSAPHGLRLVIEDYPYAVDGLEIWDAIKTWVHEYVSVYYPTNAAIQQDTELQAWWKEVVEKGHGDLKDKPWWPKLQTVEDLIQSCSIIIWTASALHAAVNFGQYPYGGYIVNRPTLARRFIPEEGTKEYDEMVKDPQKAYLRTITPKFETLIDISVIEILSRHASDEVYLGQRDNPNWTTDSKALEAFKKFGNKLAEIEGKITQRNNDPSLKSRHGPVQLPYTLLHRSSEEGMSFKGIPNSISI

SEQ ID NO:102脂加氧酶Glyma07g03910.1 BLAST 865aa

MFGILGGNKGHKIKGTVVLMSKNVLDFNEIVSTTQGGLVGAATGIFGAATGIVGGVVDGATAIFSRNIAIQLISATKTDGLGNGKVGKQTYLEKHLPSLPTLGDRQDAFSVYFEWDNDFGIPGAFYIKNFMQSEFFLVSVTLEDIPNHGTIHFVCNSWVYNAKSYKRDRIFFANKTYLPNETPTPLVKYRKEELENLRGDGKGERKEYDRIYDYDVYNDLGNPDKSNDLARPVLGGSSAYPYPRRGRTGRKPTTKDSKSESPSSSTYIPRDENFGHLKSSDFLTYGIKSIAQTVLPTFQSAFGLNAEFDRFDDVRGLFEGGIHLPTDALSKISPLPVLKEIFRTDGEQVLKFPPPHVIKVSKSAWMTDEEFGREMLAGVNPCLIECLQVFPPKSKLDPTVYGDQTSTITKEHLEINLGGLSVEQALSGNRLFILDHHDAFIAYLRKINDLPTAKSYATRTILFLKDDGTLKPLAIELSLPHPRGDEFGAVSRVVLPADQGAESTIWLIAKAYVVVNDSCYHQLMSHWLNTHAVIEPFVIATNRHLSVLHPIYKLLLPHYRDTMNINGLARQSLINAGGIIEQSFLPGPFAVEMSSAVYKGWVFTDQALPADLIKRGMAVEDPSSPYGLRLVIDDYPYAVDGLEIWSAIQTWVKDYVSLYYATDDAVKKDSELQAWWKEAVEKGHGDLKDKPWWPKLNTLQDLIHICCIIIWTASALHAAVNFGQYPYGGFILNRPTLTRRLLPEPGTKEYGELTSNHQKAYLRTITGKTEALVDLTVIEILSRHASDEVYLGQRDNPNWTDDTKAIQAFKKFGNKLKEIEDKISGRNKNSSLRNRNGPAQMPYTVLLPTSGEGLTFRGIPNSISI

SEQ ID NO:103脂加氧酶Glyma07g03920.2 BLAST 868aa

MLIGSLLNRRPKIKGTVVLMTKNVFDVNDFMATTRGGPAAVAGGIFGAAQDIVGGIVDGATAIFSRNIAIQLISATKSENALGHGKVGKLTYLEKHLPSLPNLGDRQDAFDVYFEWDESFGIPGAFYIKNYMQSEFFLVSFKLEDVPNHGTILFACNSWVYNAKLYKKDRIFFANKAYLPNDTPTPLVKYRKEELENLRGDGRGERKELDRIYDYDVYNDLGNPDENDDLARPILGGSSKHPYPRRGRTGRKPTKKDPRCERPTSDTYIPRDENFGHLKSSDFLTYAIKSLTQNVLPQFNTAFGFNNEFDSFEDVRCLFDGGVYLPTDVLSKISPIPVLKEIFRTDGEQALKFPPPHVIKVRESEWMTDEEFGREMLAGVNPGMIQRLQEFPPKSKLDPTEFGDQTSTITKEHLEINLGGLTVEQALKGNKLFILDHHDAFIPFMNLINGLPTAKSYATRTILFLQDDGTLKPLAIELSLPHPRGHEFGADSRVVLPPAAVNSAEGTIWLIAKAYVAVNDTGYHQLISHWLNTHATIEPFVIATNRHLSVLHPIHKLLLPHYRDTMNINALARQSLINADGVIERSFLPGKYSLEMSSAVYKSWVFTDQALPADLIKRGMAIEDPCAPHGLRLVIEDYPYAVDGLEIWDAIQTWVKNYVSLYYPTDDAIKKDSELQAWWKEAVETGHGDLKDKPWWPKLNTPQDLVHICSIIIWIASALHAAVNFGQYPYGGLILNRPTLTRRFLPEPGSKEYEELSTNYQKAYLRTITRKIEALVDLSVIEILSRHASDEIYLGKRDSDDWTDDQKAIQAFEKFGTKLKEIEAKINSRNKDSSLRNRNGPVQMPYTVLLPTSEEGLTFRGIPNSISI

SEQ ID NO:104脂加氧酶Glyma08g20190.1 BLAST 860aa

MYSGVKGLFNRSQKVKGTVVLMRKNVLDINSITSVRGLIGTGINIIGSTIDGLTSFLGRSVCLQLISATKADGNGNGVVGKKTYLEGIITSIPTLGAGQSAFTIHFEWDADMGIPGAFLIKNYMQVELFLVSLTLEDIPNQGSMHFVCNSWVYNSKVYEKDRIFFASETYVPSETPGPLVTYREAELQALRGNGTGKRKEWDRVYDYDVYNDLGNPDSGENFARPVLGGSLTHPYPRRGRTGRKPTKKDPNSEKPGEAYIPRDENFGHLKSSDFLTYGLKSLTRSFLPALKTVFDINFTPNEFDSFEEVRALCEGGIKLPTDILSKISPLPVLKEIFRTDGESVLKFSVPDLIKVSKSAWMTDEEFAREMIAGVNPCVIRRLQEFPPQSKLDPSVYGDQTSKMTIDHLEINLEGLTVDKAIKDQRLFILDHHDTFMPFLRRIDESKSSKAYATRTILFLKDDGTLKPLAIELSLPHPGQQQLGAYSKVILPANQGVESTIWLLAKAHVIVNDSCYHQLISHWLNTHAVIEPFVIATNRNLSILHPIYKLLFPHYRDTMNINALARQSLINADGFIEKTFLGGKYAVEISSSGYKNWVFLDQALPADLIKRGMAIEDSSCPNGLRLVIEDYPYAVDGLEIWDAIKTWVQEYVSLYYATNDAIKKDHELQAWWKEVVEKGHGDLKDKPWWPKMQTLQELIQSCSTIIWIASALHAAVNFGQYPYGGFILNRPTLSRRWIPEEGTPEYDEMTKNPQKAYLRTITPKFQALVDLSVIEILSRHASDEVYLGQRDNPNWTSNPKAIEAFKKFGKKLAEIETKISERNHDPNLRNRTGPAQLPYTVLLPTSETGLTFRGIPNSISI

SEQ ID NO:105 SSDFLTYGIK

SEQ ID NO:106 GTVVLMPK

SEQ ID NO:107 NVLDFNAITSIGK

SEQ ID NO:108 GGVIDTATGILGQGVSLVGGVIDTATSFLGR

SEQ ID NO:109 IFFVNDTYLPSATPAPLLK

SEQ ID NO:110 DENFGHLK

SEQ ID NO:111 SLSHDVIPLFK

SEQ ID NO:112 SLYEGGIK

SEQ ID NO:113 TDGENVLQFPPPHVAK

SEQ ID NO:114 INSLPTAK

SEQ ID NO:115 TILFLK

SEQ ID NO:116 HLSVLHPIYK

SEQ ID NO:117 QSLINADGIIEK

SEQ ID NO:118 FIPAEGTPEYDEMVK

SEQ ID NO:119 ALEAFK

SEQ ID NO:120 GIPNSISI

序列表

<110> T·J·阿曼

　　　B·W·谢弗

　　　R·C·希尔

　　　G·单

<120> 用于变应原的选择性定量与检测的系统和方法

<130> 2971-12526.1PC (76268-WO-PCT)

<160> 120

<170> PatentIn version 3.5

<210> 1

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> Gly m 1的示例性特征肽

<400> 1

Ser Tyr Pro Ser Asn Ala Thr Cys Pro Arg

1 5 10

<210> 2

<211> 13

<212> PRT

<213> 人工序列

<220>

<223> Gly m 3的示例性特征肽

<400> 2

Tyr Met Val Ile Gln Gly Glu Pro Gly Ala Val Ile Arg

1 5 10

<210> 3

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> Gly m 5 (beta-伴大豆球蛋白)的示例性特征肽

<400> 3

Asn Ile Leu Glu Ala Ser Tyr Asp Thr Lys

1 5 10

<210> 4

<211> 7

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 (大豆球蛋白) G2的示例性特征肽

<400> 4

Val Thr Ala Pro Ala Met Arg

1 5

<210> 5

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 (大豆球蛋白) G3的示例性特征肽

<400> 5

Asn Asn Asn Pro Phe Ser Phe Leu Val Pro Pro Lys

1 5 10

<210> 6

<211> 7

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 (大豆球蛋白)前体的示例性特征肽

<400> 6

Ala Asp Phe Tyr Asn Pro Lys

1 5

<210> 7

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> Kunitz胰蛋白酶抑制剂1的示例性特征肽

<400> 7

Gly Gly Gly Ile Glu Val Asp Ser Thr Gly Lys

1 5 10

<210> 8

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> Kunitz胰蛋白酶抑制剂3的示例性特征肽

<400> 8

Gly Ile Gly Thr Ile Ile Ser Ser Pro Tyr Arg

1 5 10

<210> 9

<211> 14

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K的示例性特征肽

<400> 9

Asn Lys Pro Gln Phe Leu Ala Gly Ala Ala Ser Leu Leu Arg

1 5 10

<210> 10

<211> 7

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 30 K的示例性特征肽

<400> 10

Gly Val Ile Thr Gln Val Lys

1 5

<210> 11

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> Gly m 8 (2S albumin)的示例性特征肽

<400> 11

Ile Met Glu Asn Gln Ser Glu Glu Leu Glu Glu Lys

1 5 10

<210> 12

<211> 119

<212> PRT

<213> 人工序列

<220>

<223> Gly m 1 ABA54898.1 [MW= 12482.64 Da]

<400> 12

Met Gly Ser Lys Val Val Ala Ser Val Ala Leu Leu Leu Ser Ile Asn

1 5 10 15

Ile Leu Phe Ile Ser Met Val Ser Ser Ser Ser His Tyr Asp Pro Gln

20 25 30

Pro Gln Pro Ser His Val Thr Ala Leu Ile Thr Arg Pro Ser Cys Pro

35 40 45

Asp Leu Ser Ile Cys Leu Asn Ile Leu Gly Gly Ser Leu Gly Thr Val

50 55 60

Asp Asp Cys Cys Ala Leu Ile Gly Gly Leu Gly Asp Ile Glu Ala Ile

65 70 75 80

Val Cys Leu Cys Ile Gln Leu Arg Ala Leu Gly Ile Leu Asn Leu Asn

85 90 95

Arg Asn Leu Gln Leu Ile Leu Asn Ser Cys Gly Arg Ser Tyr Pro Ser

100 105 110

Asn Ala Thr Cys Pro Arg Thr

115

<210> 13

<211> 131

<212> PRT

<213> 人工序列

<220>

<223> Gly m 3 CAA11755.1 [MW= 14100.07 Da]

<400> 13

Met Ser Trp Gln Ala Tyr Val Asp Asp His Leu Leu Cys Gly Ile Glu

1 5 10 15

Gly Asn His Leu Thr His Ala Ala Ile Ile Gly Gln Asp Gly Ser Val

20 25 30

Trp Leu Gln Ser Thr Asp Phe Pro Gln Phe Lys Pro Glu Glu Ile Thr

35 40 45

Ala Ile Met Asn Asp Phe Asn Glu Pro Gly Ser Leu Ala Pro Thr Gly

50 55 60

Leu Tyr Leu Gly Gly Thr Lys Tyr Met Val Ile Gln Gly Glu Pro Gly

65 70 75 80

Ala Val Ile Arg Gly Lys Lys Gly Pro Gly Gly Val Thr Val Lys Lys

85 90 95

Thr Gly Ala Ala Leu Ile Ile Gly Ile Tyr Asp Glu Pro Met Thr Pro

100 105 110

Gly Gln Cys Asn Met Val Val Glu Arg Leu Gly Asp Tyr Leu Ile Asp

115 120 125

Gln Gly Tyr

130

<210> 14

<211> 158

<212> PRT

<213> 人工序列

<220>

<223> Gly m 4 P26987 [MW= 16771.81 Da]

<400> 14

Met Gly Val Phe Thr Phe Glu Asp Glu Ile Asn Ser Pro Val Ala Pro

1 5 10 15

Ala Thr Leu Tyr Lys Ala Leu Val Thr Asp Ala Asp Asn Val Ile Pro

20 25 30

Lys Ala Leu Asp Ser Phe Lys Ser Val Glu Asn Val Glu Gly Asn Gly

35 40 45

Gly Pro Gly Thr Ile Lys Lys Ile Thr Phe Leu Glu Asp Gly Glu Thr

50 55 60

Lys Phe Val Leu His Lys Ile Glu Ser Ile Asp Glu Ala Asn Leu Gly

65 70 75 80

Tyr Ser Tyr Ser Val Val Gly Gly Ala Ala Leu Pro Asp Thr Ala Glu

85 90 95

Lys Ile Thr Phe Asp Ser Lys Leu Val Ala Gly Pro Asn Gly Gly Ser

100 105 110

Ala Gly Lys Leu Thr Val Lys Tyr Glu Thr Lys Gly Asp Ala Glu Pro

115 120 125

Asn Gln Asp Glu Leu Lys Thr Gly Lys Ala Lys Ala Asp Ala Leu Phe

130 135 140

Lys Ala Ile Glu Ala Tyr Leu Leu Ala His Pro Asp Tyr Asn

145 150 155

<210> 15

<211> 605

<212> PRT

<213> 人工序列

<220>

<223> Gly m 5 (beta-伴大豆球蛋白) 121281 [MW= 70293.13 Da]

<400> 15

Met Met Arg Ala Arg Phe Pro Leu Leu Leu Leu Gly Leu Val Phe Leu

1 5 10 15

Ala Ser Val Ser Val Ser Phe Gly Ile Ala Tyr Trp Glu Lys Glu Asn

20 25 30

Pro Lys His Asn Lys Cys Leu Gln Ser Cys Asn Ser Glu Arg Asp Ser

35 40 45

Tyr Arg Asn Gln Ala Cys His Ala Arg Cys Asn Leu Leu Lys Val Glu

50 55 60

Lys Glu Glu Cys Glu Glu Gly Glu Ile Pro Arg Pro Arg Pro Arg Pro

65 70 75 80

Gln His Pro Glu Arg Glu Pro Gln Gln Pro Gly Glu Lys Glu Glu Asp

85 90 95

Glu Asp Glu Gln Pro Arg Pro Ile Pro Phe Pro Arg Pro Gln Pro Arg

100 105 110

Gln Glu Glu Glu His Glu Gln Arg Glu Glu Gln Glu Trp Pro Arg Lys

115 120 125

Glu Glu Lys Arg Gly Glu Lys Gly Ser Glu Glu Glu Asp Glu Asp Glu

130 135 140

Asp Glu Glu Gln Asp Glu Arg Gln Phe Pro Phe Pro Arg Pro Pro His

145 150 155 160

Gln Lys Glu Glu Arg Asn Glu Glu Glu Asp Glu Asp Glu Glu Gln Gln

165 170 175

Arg Glu Ser Glu Glu Ser Glu Asp Ser Glu Leu Arg Arg His Lys Asn

180 185 190

Lys Asn Pro Phe Leu Phe Gly Ser Asn Arg Phe Glu Thr Leu Phe Lys

195 200 205

Asn Gln Tyr Gly Arg Ile Arg Val Leu Gln Arg Phe Asn Gln Arg Ser

210 215 220

Pro Gln Leu Gln Asn Leu Arg Asp Tyr Arg Ile Leu Glu Phe Asn Ser

225 230 235 240

Lys Pro Asn Thr Leu Leu Leu Pro Asn His Ala Asp Ala Asp Tyr Leu

245 250 255

Ile Val Ile Leu Asn Gly Thr Ala Ile Leu Ser Leu Val Asn Asn Asp

260 265 270

Asp Arg Asp Ser Tyr Arg Leu Gln Ser Gly Asp Ala Leu Arg Val Pro

275 280 285

Ser Gly Thr Thr Tyr Tyr Val Val Asn Pro Asp Asn Asn Glu Asn Leu

290 295 300

Arg Leu Ile Thr Leu Ala Ile Pro Val Asn Lys Pro Gly Arg Phe Glu

305 310 315 320

Ser Phe Phe Leu Ser Ser Thr Glu Ala Gln Gln Ser Tyr Leu Gln Gly

325 330 335

Phe Ser Arg Asn Ile Leu Glu Ala Ser Tyr Asp Thr Lys Phe Glu Glu

340 345 350

Ile Asn Lys Val Leu Phe Ser Arg Glu Glu Gly Gln Gln Gln Gly Glu

355 360 365

Gln Arg Leu Gln Glu Ser Val Ile Val Glu Ile Ser Lys Glu Gln Ile

370 375 380

Arg Ala Leu Ser Lys Arg Ala Lys Ser Ser Ser Arg Lys Thr Ile Ser

385 390 395 400

Ser Glu Asp Lys Pro Phe Asn Leu Arg Ser Arg Asp Pro Ile Tyr Ser

405 410 415

Asn Lys Leu Gly Lys Phe Phe Glu Ile Thr Pro Glu Lys Asn Pro Gln

420 425 430

Leu Arg Asp Leu Asp Ile Phe Leu Ser Ile Val Asp Met Asn Glu Gly

435 440 445

Ala Leu Leu Leu Pro His Phe Asn Ser Lys Ala Ile Val Ile Leu Val

450 455 460

Ile Asn Glu Gly Asp Ala Asn Ile Glu Leu Val Gly Leu Lys Glu Gln

465 470 475 480

Gln Gln Glu Gln Gln Gln Glu Glu Gln Pro Leu Glu Val Arg Lys Tyr

485 490 495

Arg Ala Glu Leu Ser Glu Gln Asp Ile Phe Val Ile Pro Ala Gly Tyr

500 505 510

Pro Val Val Val Asn Ala Thr Ser Asn Leu Asn Phe Phe Ala Ile Gly

515 520 525

Ile Asn Ala Glu Asn Asn Gln Arg Asn Phe Leu Ala Gly Ser Gln Asp

530 535 540

Asn Val Ile Ser Gln Ile Pro Ser Gln Val Gln Glu Leu Ala Phe Pro

545 550 555 560

Gly Ser Ala Gln Ala Val Glu Lys Leu Leu Lys Asn Gln Arg Glu Ser

565 570 575

Tyr Phe Val Asp Ala Gln Pro Lys Lys Lys Glu Glu Gly Asn Lys Gly

580 585 590

Arg Lys Gly Pro Leu Ser Ser Ile Leu Arg Ala Phe Tyr

595 600 605

<210> 16

<211> 495

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 大豆球蛋白 G1 121276 [MW= 55706.34 Da]

<400> 16

Met Ala Lys Leu Val Phe Ser Leu Cys Phe Leu Leu Phe Ser Gly Cys

1 5 10 15

Cys Phe Ala Phe Ser Ser Arg Glu Gln Pro Gln Gln Asn Glu Cys Gln

20 25 30

Ile Gln Lys Leu Asn Ala Leu Lys Pro Asp Asn Arg Ile Glu Ser Glu

35 40 45

Gly Gly Leu Ile Glu Thr Trp Asn Pro Asn Asn Lys Pro Phe Gln Cys

50 55 60

Ala Gly Val Ala Leu Ser Arg Cys Thr Leu Asn Arg Asn Ala Leu Arg

65 70 75 80

Arg Pro Ser Tyr Thr Asn Gly Pro Gln Glu Ile Tyr Ile Gln Gln Gly

85 90 95

Lys Gly Ile Phe Gly Met Ile Tyr Pro Gly Cys Pro Ser Thr Phe Glu

100 105 110

Glu Pro Gln Gln Pro Gln Gln Arg Gly Gln Ser Ser Arg Pro Gln Asp

115 120 125

Arg His Gln Lys Ile Tyr Asn Phe Arg Glu Gly Asp Leu Ile Ala Val

130 135 140

Pro Thr Gly Val Ala Trp Trp Met Tyr Asn Asn Glu Asp Thr Pro Val

145 150 155 160

Val Ala Val Ser Ile Ile Asp Thr Asn Ser Leu Glu Asn Gln Leu Asp

165 170 175

Gln Met Pro Arg Arg Phe Tyr Leu Ala Gly Asn Gln Glu Gln Glu Phe

180 185 190

Leu Lys Tyr Gln Gln Glu Gln Gly Gly His Gln Ser Gln Lys Gly Lys

195 200 205

His Gln Gln Glu Glu Glu Asn Glu Gly Gly Ser Ile Leu Ser Gly Phe

210 215 220

Thr Leu Glu Phe Leu Glu His Ala Phe Ser Val Asp Lys Gln Ile Ala

225 230 235 240

Lys Asn Leu Gln Gly Glu Asn Glu Gly Glu Asp Lys Gly Ala Ile Val

245 250 255

Thr Val Lys Gly Gly Leu Ser Val Ile Lys Pro Pro Thr Asp Glu Gln

260 265 270

Gln Gln Arg Pro Gln Glu Glu Glu Glu Glu Glu Glu Asp Glu Lys Pro

275 280 285

Gln Cys Lys Gly Lys Asp Lys His Cys Gln Arg Pro Arg Gly Ser Gln

290 295 300

Ser Lys Ser Arg Arg Asn Gly Ile Asp Glu Thr Ile Cys Thr Met Arg

305 310 315 320

Leu Arg His Asn Ile Gly Gln Thr Ser Ser Pro Asp Ile Tyr Asn Pro

325 330 335

Gln Ala Gly Ser Val Thr Thr Ala Thr Ser Leu Asp Phe Pro Ala Leu

340 345 350

Ser Trp Leu Arg Leu Ser Ala Glu Phe Gly Ser Leu Arg Lys Asn Ala

355 360 365

Met Phe Val Pro His Tyr Asn Leu Asn Ala Asn Ser Ile Ile Tyr Ala

370 375 380

Leu Asn Gly Arg Ala Leu Ile Gln Val Val Asn Cys Asn Gly Glu Arg

385 390 395 400

Val Phe Asp Gly Glu Leu Gln Glu Gly Arg Val Leu Ile Val Pro Gln

405 410 415

Asn Phe Val Val Ala Ala Arg Ser Gln Ser Asp Asn Phe Glu Tyr Val

420 425 430

Ser Phe Lys Thr Asn Asp Thr Pro Met Ile Gly Thr Leu Ala Gly Ala

435 440 445

Asn Ser Leu Leu Asn Ala Leu Pro Glu Glu Val Ile Gln His Thr Phe

450 455 460

Asn Leu Lys Ser Gln Gln Ala Arg Gln Ile Lys Asn Asn Asn Pro Phe

465 470 475 480

Lys Phe Leu Val Pro Pro Gln Glu Ser Gln Lys Arg Ala Val Ala

485 490 495

<210> 17

<211> 485

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 大豆球蛋白 G2 121277 [MW= 54390.76 Da]

<400> 17

Met Ala Lys Leu Val Leu Ser Leu Cys Phe Leu Leu Phe Ser Gly Cys

1 5 10 15

Phe Ala Leu Arg Glu Gln Ala Gln Gln Asn Glu Cys Gln Ile Gln Lys

20 25 30

Leu Asn Ala Leu Lys Pro Asp Asn Arg Ile Glu Ser Glu Gly Gly Phe

35 40 45

Ile Glu Thr Trp Asn Pro Asn Asn Lys Pro Phe Gln Cys Ala Gly Val

50 55 60

Ala Leu Ser Arg Cys Thr Leu Asn Arg Asn Ala Leu Arg Arg Pro Ser

65 70 75 80

Tyr Thr Asn Gly Pro Gln Glu Ile Tyr Ile Gln Gln Gly Asn Gly Ile

85 90 95

Phe Gly Met Ile Phe Pro Gly Cys Pro Ser Thr Tyr Gln Glu Pro Gln

100 105 110

Glu Ser Gln Gln Arg Gly Arg Ser Gln Arg Pro Gln Asp Arg His Gln

115 120 125

Lys Val His Arg Phe Arg Glu Gly Asp Leu Ile Ala Val Pro Thr Gly

130 135 140

Val Ala Trp Trp Met Tyr Asn Asn Glu Asp Thr Pro Val Val Ala Val

145 150 155 160

Ser Ile Ile Asp Thr Asn Ser Leu Glu Asn Gln Leu Asp Gln Met Pro

165 170 175

Arg Arg Phe Tyr Leu Ala Gly Asn Gln Glu Gln Glu Phe Leu Lys Tyr

180 185 190

Gln Gln Gln Gln Gln Gly Gly Ser Gln Ser Gln Lys Gly Lys Gln Gln

195 200 205

Glu Glu Glu Asn Glu Gly Ser Asn Ile Leu Ser Gly Phe Ala Pro Glu

210 215 220

Phe Leu Lys Glu Ala Phe Gly Val Asn Met Gln Ile Val Arg Asn Leu

225 230 235 240

Gln Gly Glu Asn Glu Glu Glu Asp Ser Gly Ala Ile Val Thr Val Lys

245 250 255

Gly Gly Leu Arg Val Thr Ala Pro Ala Met Arg Lys Pro Gln Gln Glu

260 265 270

Glu Asp Asp Asp Asp Glu Glu Glu Gln Pro Gln Cys Val Glu Thr Asp

275 280 285

Lys Gly Cys Gln Arg Gln Ser Lys Arg Ser Arg Asn Gly Ile Asp Glu

290 295 300

Thr Ile Cys Thr Met Arg Leu Arg Gln Asn Ile Gly Gln Asn Ser Ser

305 310 315 320

Pro Asp Ile Tyr Asn Pro Gln Ala Gly Ser Ile Thr Thr Ala Thr Ser

325 330 335

Leu Asp Phe Pro Ala Leu Trp Leu Leu Lys Leu Ser Ala Gln Tyr Gly

340 345 350

Ser Leu Arg Lys Asn Ala Met Phe Val Pro His Tyr Thr Leu Asn Ala

355 360 365

Asn Ser Ile Ile Tyr Ala Leu Asn Gly Arg Ala Leu Val Gln Val Val

370 375 380

Asn Cys Asn Gly Glu Arg Val Phe Asp Gly Glu Leu Gln Glu Gly Gly

385 390 395 400

Val Leu Ile Val Pro Gln Asn Phe Ala Val Ala Ala Lys Ser Gln Ser

405 410 415

Asp Asn Phe Glu Tyr Val Ser Phe Lys Thr Asn Asp Arg Pro Ser Ile

420 425 430

Gly Asn Leu Ala Gly Ala Asn Ser Leu Leu Asn Ala Leu Pro Glu Glu

435 440 445

Val Ile Gln His Thr Phe Asn Leu Lys Ser Gln Gln Ala Arg Gln Val

450 455 460

Lys Asn Asn Asn Pro Phe Ser Phe Leu Val Pro Pro Gln Glu Ser Gln

465 470 475 480

Arg Arg Ala Val Ala

485

<210> 18

<211> 481

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 大豆球蛋白 G3 121278 [MW= 54241.73 Da]

<400> 18

Met Ala Lys Leu Val Leu Ser Leu Cys Phe Leu Leu Phe Ser Gly Cys

1 5 10 15

Cys Phe Ala Phe Ser Phe Arg Glu Gln Pro Gln Gln Asn Glu Cys Gln

20 25 30

Ile Gln Arg Leu Asn Ala Leu Lys Pro Asp Asn Arg Ile Glu Ser Glu

35 40 45

Gly Gly Phe Ile Glu Thr Trp Asn Pro Asn Asn Lys Pro Phe Gln Cys

50 55 60

Ala Gly Val Ala Leu Ser Arg Cys Thr Leu Asn Arg Asn Ala Leu Arg

65 70 75 80

Arg Pro Ser Tyr Thr Asn Ala Pro Gln Glu Ile Tyr Ile Gln Gln Gly

85 90 95

Ser Gly Ile Phe Gly Met Ile Phe Pro Gly Cys Pro Ser Thr Phe Glu

100 105 110

Glu Pro Gln Gln Lys Gly Gln Ser Ser Arg Pro Gln Asp Arg His Gln

115 120 125

Lys Ile Tyr His Phe Arg Glu Gly Asp Leu Ile Ala Val Pro Thr Gly

130 135 140

Phe Ala Tyr Trp Met Tyr Asn Asn Glu Asp Thr Pro Val Val Ala Val

145 150 155 160

Ser Leu Ile Asp Thr Asn Ser Phe Gln Asn Gln Leu Asp Gln Met Pro

165 170 175

Arg Arg Phe Tyr Leu Ala Gly Asn Gln Glu Gln Glu Phe Leu Gln Tyr

180 185 190

Gln Pro Gln Lys Gln Gln Gly Gly Thr Gln Ser Gln Lys Gly Lys Arg

195 200 205

Gln Gln Glu Glu Glu Asn Glu Gly Gly Ser Ile Leu Ser Gly Phe Ala

210 215 220

Pro Glu Phe Leu Glu His Ala Phe Val Val Asp Arg Gln Ile Val Arg

225 230 235 240

Lys Leu Gln Gly Glu Asn Glu Glu Glu Glu Lys Gly Ala Ile Val Thr

245 250 255

Val Lys Gly Gly Leu Ser Val Ile Ser Pro Pro Thr Glu Glu Gln Gln

260 265 270

Gln Arg Pro Glu Glu Glu Glu Lys Pro Asp Cys Asp Glu Lys Asp Lys

275 280 285

His Cys Gln Ser Gln Ser Arg Asn Gly Ile Asp Glu Thr Ile Cys Thr

290 295 300

Met Arg Leu Arg His Asn Ile Gly Gln Thr Ser Ser Pro Asp Ile Phe

305 310 315 320

Asn Pro Gln Ala Gly Ser Ile Thr Thr Ala Thr Ser Leu Asp Phe Pro

325 330 335

Ala Leu Ser Trp Leu Lys Leu Ser Ala Gln Phe Gly Ser Leu Arg Lys

340 345 350

Asn Ala Met Phe Val Pro His Tyr Asn Leu Asn Ala Asn Ser Ile Ile

355 360 365

Tyr Ala Leu Asn Gly Arg Ala Leu Val Gln Val Val Asn Cys Asn Gly

370 375 380

Glu Arg Val Phe Asp Gly Glu Leu Gln Glu Gly Gln Val Leu Ile Val

385 390 395 400

Pro Gln Asn Phe Ala Val Ala Ala Arg Ser Gln Ser Asp Asn Phe Glu

405 410 415

Tyr Val Ser Phe Lys Thr Asn Asp Arg Pro Ser Ile Gly Asn Leu Ala

420 425 430

Gly Ala Asn Ser Leu Leu Asn Ala Leu Pro Glu Glu Val Ile Gln Gln

435 440 445

Thr Phe Asn Leu Arg Arg Gln Gln Ala Arg Gln Val Lys Asn Asn Asn

450 455 460

Pro Phe Ser Phe Leu Val Pro Pro Lys Glu Ser Gln Arg Arg Val Val

465 470 475 480

Ala

<210> 19

<211> 562

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 大豆球蛋白 G4 121279 [MW= 63587.16 Da]

<400> 19

Met Gly Lys Pro Phe Thr Leu Ser Leu Ser Ser Leu Cys Leu Leu Leu

1 5 10 15

Leu Ser Ser Ala Cys Phe Ala Ile Ser Ser Ser Lys Leu Asn Glu Cys

20 25 30

Gln Leu Asn Asn Leu Asn Ala Leu Glu Pro Asp His Arg Val Glu Ser

35 40 45

Glu Gly Gly Leu Ile Gln Thr Trp Asn Ser Gln His Pro Glu Leu Lys

50 55 60

Cys Ala Gly Val Thr Val Ser Lys Leu Thr Leu Asn Arg Asn Gly Leu

65 70 75 80

His Ser Pro Ser Tyr Ser Pro Tyr Pro Arg Met Ile Ile Ile Ala Gln

85 90 95

Gly Lys Gly Ala Leu Gly Val Ala Ile Pro Gly Cys Pro Glu Thr Phe

100 105 110

Glu Glu Pro Gln Glu Gln Ser Asn Arg Arg Gly Ser Arg Ser Gln Lys

115 120 125

Gln Gln Leu Gln Asp Ser His Gln Lys Ile Arg His Phe Asn Glu Gly

130 135 140

Asp Val Leu Val Ile Pro Pro Ser Val Pro Tyr Trp Thr Tyr Asn Thr

145 150 155 160

Gly Asp Glu Pro Val Val Ala Ile Ser Leu Leu Asp Thr Ser Asn Phe

165 170 175

Asn Asn Gln Leu Asp Gln Thr Pro Arg Val Phe Tyr Leu Ala Gly Asn

180 185 190

Pro Asp Ile Glu Tyr Pro Glu Thr Met Gln Gln Gln Gln Gln Gln Lys

195 200 205

Ser His Gly Gly Arg Lys Gln Gly Gln His Gln Gln Glu Glu Glu Glu

210 215 220

Glu Gly Gly Ser Val Leu Ser Gly Phe Ser Lys His Phe Leu Ala Gln

225 230 235 240

Ser Phe Asn Thr Asn Glu Asp Ile Ala Glu Lys Leu Glu Ser Pro Asp

245 250 255

Asp Glu Arg Lys Gln Ile Val Thr Val Glu Gly Gly Leu Ser Val Ile

260 265 270

Ser Pro Lys Trp Gln Glu Gln Gln Asp Glu Asp Glu Asp Glu Asp Glu

275 280 285

Asp Asp Glu Asp Glu Gln Ile Pro Ser His Pro Pro Arg Arg Pro Ser

290 295 300

His Gly Lys Arg Glu Gln Asp Glu Asp Glu Asp Glu Asp Glu Asp Lys

305 310 315 320

Pro Arg Pro Ser Arg Pro Ser Gln Gly Lys Arg Asn Lys Thr Gly Gln

325 330 335

Asp Glu Asp Glu Asp Glu Asp Glu Asp Gln Pro Arg Lys Ser Arg Glu

340 345 350

Trp Arg Ser Lys Lys Thr Gln Pro Arg Arg Pro Arg Gln Glu Glu Pro

355 360 365

Arg Glu Arg Gly Cys Glu Thr Arg Asn Gly Val Glu Glu Asn Ile Cys

370 375 380

Thr Leu Lys Leu His Glu Asn Ile Ala Arg Pro Ser Arg Ala Asp Phe

385 390 395 400

Tyr Asn Pro Lys Ala Gly Arg Ile Ser Thr Leu Asn Ser Leu Thr Leu

405 410 415

Pro Ala Leu Arg Gln Phe Gln Leu Ser Ala Gln Tyr Val Val Leu Tyr

420 425 430

Lys Asn Gly Ile Tyr Ser Pro His Trp Asn Leu Asn Ala Asn Ser Val

435 440 445

Ile Tyr Val Thr Arg Gly Gln Gly Lys Val Arg Val Val Asn Cys Gln

450 455 460

Gly Asn Ala Val Phe Asp Gly Glu Leu Arg Arg Gly Gln Leu Leu Val

465 470 475 480

Val Pro Gln Asn Phe Val Val Ala Glu Gln Ala Gly Glu Gln Gly Phe

485 490 495

Glu Tyr Ile Val Phe Lys Thr His His Asn Ala Val Thr Ser Tyr Leu

500 505 510

Lys Asp Val Phe Arg Ala Ile Pro Ser Glu Val Leu Ala His Ser Tyr

515 520 525

Asn Leu Arg Gln Ser Gln Val Ser Glu Leu Lys Tyr Glu Gly Asn Trp

530 535 540

Gly Pro Leu Val Asn Pro Glu Ser Gln Gln Gly Ser Pro Arg Val Lys

545 550 555 560

Val Ala

<210> 20

<211> 562

<212> PRT

<213> 人工序列

<220>

<223> Gly m 6 大豆球蛋白前体 75221455 [MW= 63876.47 Da]

<400> 20

Met Gly Lys Pro Phe Thr Leu Ser Leu Ser Ser Leu Cys Leu Leu Leu

1 5 10 15

Leu Ser Ser Ala Cys Phe Ala Ile Ser Ser Ser Lys Leu Asn Glu Cys

20 25 30

Gln Leu Asn Asn Leu Asn Ala Leu Glu Pro Asp His Arg Val Glu Phe

35 40 45

Glu Gly Gly Leu Ile Gln Thr Trp Asn Ser Gln His Pro Glu Leu Lys

50 55 60

Cys Ala Gly Val Thr Val Ser Lys Leu Thr Leu Asn Arg Asn Gly Leu

65 70 75 80

His Leu Pro Ser Tyr Ser Pro Tyr Pro Arg Met Ile Ile Ile Ala Gln

85 90 95

Gly Lys Gly Ala Leu Gln Cys Lys Pro Gly Cys Pro Glu Thr Phe Glu

100 105 110

Glu Pro Gln Glu Gln Ser Asn Arg Arg Gly Ser Arg Ser Gln Lys Gln

115 120 125

Gln Leu Gln Asp Ser His Gln Lys Ile Arg His Phe Asn Glu Gly Asp

130 135 140

Val Leu Val Ile Pro Pro Gly Val Pro Tyr Trp Thr Tyr Asn Thr Gly

145 150 155 160

Asp Glu Pro Val Val Ala Ile Ser Leu Leu Asp Thr Ser Asn Phe Asn

165 170 175

Asn Gln Leu Asp Gln Thr Pro Arg Val Phe Tyr Leu Ala Gly Asn Pro

180 185 190

Asp Ile Glu Tyr Pro Glu Thr Met Gln Gln Gln Gln Gln Gln Lys Ser

195 200 205

His Gly Gly Arg Lys Gln Gly Gln His Gln Gln Glu Glu Glu Glu Glu

210 215 220

Gly Gly Ser Val Leu Ser Gly Phe Ser Lys His Phe Leu Ala Gln Ser

225 230 235 240

Phe Asn Thr Asn Glu Asp Ile Ala Glu Lys Leu Gln Ser Pro Asp Asp

245 250 255

Glu Arg Lys Gln Ile Val Thr Val Glu Gly Gly Leu Ser Val Ile Ser

260 265 270

Pro Lys Trp Gln Glu Gln Gln Asp Glu Asp Glu Asp Glu Asp Glu Asp

275 280 285

Asp Glu Asp Glu Gln Ile Pro Ser His Pro Pro Arg Arg Pro Ser His

290 295 300

Gly Lys Arg Glu Gln Asp Glu Asp Glu Asp Glu Asp Glu Asp Lys Pro

305 310 315 320

Arg Pro Ser Arg Pro Ser Gln Gly Lys Arg Glu Gln Asp Gln Asp Gln

325 330 335

Asp Glu Asp Glu Asp Glu Asp Glu Asp Gln Pro Arg Lys Ser Arg Glu

340 345 350

Trp Arg Ser Lys Lys Thr Gln Pro Arg Arg Pro Arg Gln Glu Glu Pro

355 360 365

Arg Glu Arg Gly Cys Glu Thr Arg Asn Gly Val Glu Glu Asn Ile Cys

370 375 380

Thr Leu Lys Leu His Glu Asn Ile Ala Arg Pro Ser Arg Ala Asp Phe

385 390 395 400

Tyr Asn Pro Lys Ala Gly Arg Ile Ser Thr Leu Asn Ser Leu Thr Leu

405 410 415

Pro Ala Leu Arg Gln Phe Gln Leu Ser Ala Gln Tyr Val Val Leu Tyr

420 425 430

Lys Asn Gly Ile Tyr Ser Pro His Trp Asn Leu Asn Ala Asn Ser Val

435 440 445

Ile Tyr Val Thr Arg Gly Gln Gly Lys Val Arg Val Val Asn Cys Gln

450 455 460

Gly Asn Ala Val Phe Asp Gly Glu Leu Arg Arg Gly Gln Leu Leu Val

465 470 475 480

Val Pro Gln Asn Phe Val Val Ala Glu Gln Ala Gly Glu Gln Gly Phe

485 490 495

Glu Tyr Ile Val Phe Lys Thr His His Asn Ala Val Thr Ser Tyr Leu

500 505 510

Lys Asp Val Phe Arg Ala Ile Pro Ser Glu Val Leu Ala His Ser Tyr

515 520 525

Asn Leu Arg Gln Ser Gln Val Ser Glu Leu Lys Tyr Glu Gly Asn Trp

530 535 540

Gly Pro Leu Val Asn Pro Glu Ser Gln Gln Gly Ser Pro Arg Val Lys

545 550 555 560

Val Ala

<210> 21

<211> 476

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28K 12697782 [MW= 52944.36 Da]

<400> 21

Met Gly Asn Lys Thr Thr Leu Leu Leu Leu Leu Phe Val Leu Cys His

1 5 10 15

Gly Val Ala Thr Thr Thr Met Ala Phe His Asp Asp Glu Gly Gly Asp

20 25 30

Lys Lys Ser Pro Lys Ser Leu Phe Leu Met Ser Asn Ser Thr Arg Val

35 40 45

Phe Lys Thr Asp Ala Gly Glu Met Arg Val Leu Lys Ser His Gly Gly

50 55 60

Arg Ile Phe Tyr Arg His Met His Ile Gly Phe Ile Ser Met Glu Pro

65 70 75 80

Lys Ser Leu Phe Val Pro Gln Tyr Leu Asp Ser Asn Leu Ile Ile Phe

85 90 95

Ile Arg Arg Gly Glu Ala Lys Leu Gly Phe Ile Tyr Asp Asp Glu Leu

100 105 110

Ala Glu Arg Arg Leu Lys Thr Gly Asp Leu Tyr Met Ile Pro Ser Gly

115 120 125

Ser Ala Phe Tyr Leu Val Asn Ile Gly Glu Gly Gln Arg Leu His Val

130 135 140

Ile Cys Ser Ile Asp Pro Ser Thr Ser Leu Gly Leu Glu Thr Phe Gln

145 150 155 160

Ser Phe Tyr Ile Gly Gly Gly Ala Asn Ser His Ser Val Leu Ser Gly

165 170 175

Phe Glu Pro Ala Ile Leu Glu Thr Ala Phe Asn Glu Ser Arg Thr Val

180 185 190

Val Glu Glu Ile Phe Ser Lys Glu Leu Asp Gly Pro Ile Met Phe Val

195 200 205

Asp Asp Ser His Ala Pro Ser Leu Trp Thr Lys Phe Leu Gln Leu Lys

210 215 220

Lys Asp Asp Lys Glu Gln Gln Leu Lys Lys Met Met Gln Asp Gln Glu

225 230 235 240

Glu Asp Glu Glu Glu Lys Gln Thr Ser Arg Ser Trp Arg Lys Leu Leu

245 250 255

Glu Thr Val Phe Gly Lys Val Asn Glu Lys Ile Glu Asn Lys Asp Thr

260 265 270

Ala Gly Ser Pro Ala Ser Tyr Asn Leu Tyr Asp Asp Lys Lys Ala Asp

275 280 285

Phe Lys Asn Ala Tyr Gly Trp Ser Lys Ala Leu His Gly Gly Glu Tyr

290 295 300

Pro Pro Leu Ser Glu Pro Asp Ile Gly Val Leu Leu Val Lys Leu Ser

305 310 315 320

Ala Gly Ser Met Leu Ala Pro His Val Asn Pro Ile Ser Asp Glu Tyr

325 330 335

Thr Ile Val Leu Ser Gly Tyr Gly Glu Leu His Ile Gly Tyr Pro Asn

340 345 350

Gly Ser Arg Ala Met Lys Thr Lys Ile Lys Gln Gly Asp Val Phe Val

355 360 365

Val Pro Arg Tyr Phe Pro Phe Cys Gln Val Ala Ser Arg Asp Gly Pro

370 375 380

Leu Glu Phe Phe Gly Phe Ser Thr Ser Ala Arg Lys Asn Lys Pro Gln

385 390 395 400

Phe Leu Ala Gly Ala Ala Ser Leu Leu Arg Thr Leu Met Gly Pro Glu

405 410 415

Leu Ser Ala Ala Phe Gly Val Ser Glu Asp Thr Leu Arg Arg Ala Val

420 425 430

Asp Ala Gln His Glu Ala Val Ile Leu Pro Ser Ala Trp Ala Ala Pro

435 440 445

Pro Glu Asn Ala Gly Lys Leu Lys Met Glu Glu Glu Pro Asn Ala Ile

450 455 460

Arg Ser Phe Ala Asn Asp Val Val Met Asp Val Phe

465 470 475

<210> 22

<211> 379

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 30K 84371705 [MW= 42757.81 Da]

<400> 22

Met Gly Phe Leu Val Leu Leu Leu Phe Ser Leu Leu Gly Leu Ser Ser

1 5 10 15

Ser Ser Ser Ile Ser Thr His Arg Ser Ile Leu Asp Leu Asp Leu Thr

20 25 30

Lys Phe Thr Thr Gln Lys Gln Val Ser Ser Leu Phe Gln Leu Trp Lys

35 40 45

Ser Glu His Gly Arg Val Tyr His Asn His Glu Glu Glu Ala Lys Arg

50 55 60

Leu Glu Ile Phe Lys Asn Asn Leu Asn Tyr Ile Arg Asp Met Asn Ala

65 70 75 80

Asn Arg Lys Ser Pro His Ser His Arg Leu Gly Leu Asn Lys Phe Ala

85 90 95

Asp Ile Thr Pro Gln Glu Phe Ser Lys Lys Tyr Leu Gln Ala Pro Lys

100 105 110

Asp Val Ser Gln Gln Ile Lys Met Ala Asn Lys Lys Met Lys Lys Glu

115 120 125

Gln Tyr Ser Cys Asp His Pro Pro Ala Ser Trp Asp Trp Arg Lys Lys

130 135 140

Gly Val Ile Thr Gln Val Lys Tyr Gln Gly Gly Cys Gly Ser Gly Trp

145 150 155 160

Ala Phe Ser Ala Thr Gly Ala Ile Glu Ala Ala His Ala Ile Ala Thr

165 170 175

Gly Asp Leu Val Ser Leu Ser Glu Gln Glu Leu Val Asp Cys Val Glu

180 185 190

Glu Ser Glu Gly Cys Tyr Asn Gly Trp His Tyr Gln Ser Phe Glu Trp

195 200 205

Val Leu Glu His Gly Gly Ile Ala Thr Asp Asp Asp Tyr Pro Tyr Arg

210 215 220

Ala Lys Glu Gly Arg Cys Lys Ala Asn Lys Ile Gln Asp Lys Val Thr

225 230 235 240

Ile Asp Gly Tyr Glu Thr Leu Ile Met Ser Asp Glu Ser Thr Glu Ser

245 250 255

Glu Thr Glu Gln Ala Phe Leu Ser Ala Ile Leu Glu Gln Pro Ile Ser

260 265 270

Val Ser Ile Asp Ala Lys Asp Phe His Leu Tyr Thr Gly Gly Ile Tyr

275 280 285

Asp Gly Glu Asn Cys Thr Ser Pro Tyr Gly Ile Asn His Phe Val Leu

290 295 300

Leu Val Gly Tyr Gly Ser Ala Asp Gly Val Asp Tyr Trp Ile Ala Lys

305 310 315 320

Asn Ser Trp Gly Glu Asp Trp Gly Glu Asp Gly Tyr Ile Trp Ile Gln

325 330 335

Arg Asn Thr Gly Asn Leu Leu Gly Val Cys Gly Met Asn Tyr Phe Ala

340 345 350

Ser Tyr Pro Thr Lys Glu Glu Ser Glu Thr Leu Val Ser Ala Arg Val

355 360 365

Lys Gly His Arg Arg Val Asp His Ser Pro Leu

370 375

<210> 23

<211> 203

<212> PRT

<213> 人工序列

<220>

<223> KTI 1 125722 [MW= 22545.94 Da]

<400> 23

Met Lys Ser Thr Ile Phe Phe Ala Leu Phe Leu Val Cys Ala Phe Thr

1 5 10 15

Ile Ser Tyr Leu Pro Ser Ala Thr Ala Gln Phe Val Leu Asp Thr Asp

20 25 30

Asp Asp Pro Leu Gln Asn Gly Gly Thr Tyr Tyr Met Leu Pro Val Met

35 40 45

Arg Gly Lys Gly Gly Gly Ile Glu Val Asp Ser Thr Gly Lys Glu Ile

50 55 60

Cys Pro Leu Thr Val Val Gln Ser Pro Asn Glu Leu Asp Lys Gly Ile

65 70 75 80

Gly Leu Val Phe Thr Ser Pro Leu His Ala Leu Phe Ile Ala Glu Arg

85 90 95

Tyr Pro Leu Ser Ile Lys Phe Gly Ser Phe Ala Val Ile Thr Leu Cys

100 105 110

Ala Gly Met Pro Thr Glu Trp Ala Ile Val Glu Arg Glu Gly Leu Gln

115 120 125

Ala Val Lys Leu Ala Ala Arg Asp Thr Val Asp Gly Trp Phe Asn Ile

130 135 140

Glu Arg Val Ser Arg Glu Tyr Asn Asp Tyr Lys Leu Val Phe Cys Pro

145 150 155 160

Gln Gln Ala Glu Asp Asn Lys Cys Glu Asp Ile Gly Ile Gln Ile Asp

165 170 175

Asp Asp Gly Ile Arg Arg Leu Val Leu Ser Lys Asn Lys Pro Leu Val

180 185 190

Val Gln Phe Gln Lys Phe Arg Ser Ser Thr Ala

195 200

<210> 24

<211> 216

<212> PRT

<213> 人工序列

<220>

<223> KTI 3 125020 [ MW= 24005.29 Da]

<400> 24

Met Lys Ser Thr Ile Phe Phe Leu Phe Leu Phe Cys Ala Phe Thr Thr

1 5 10 15

Ser Tyr Leu Pro Ser Ala Ile Ala Asp Phe Val Leu Asp Asn Glu Gly

20 25 30

Asn Pro Leu Glu Asn Gly Gly Thr Tyr Tyr Ile Leu Ser Asp Ile Thr

35 40 45

Ala Phe Gly Gly Ile Arg Ala Ala Pro Thr Gly Asn Glu Arg Cys Pro

50 55 60

Leu Thr Val Val Gln Ser Arg Asn Glu Leu Asp Lys Gly Ile Gly Thr

65 70 75 80

Ile Ile Ser Ser Pro Tyr Arg Ile Arg Phe Ile Ala Glu Gly His Pro

85 90 95

Leu Ser Leu Lys Phe Asp Ser Phe Ala Val Ile Met Leu Cys Val Gly

100 105 110

Ile Pro Thr Glu Trp Ser Val Val Glu Asp Leu Pro Glu Gly Pro Ala

115 120 125

Val Lys Ile Gly Glu Asn Lys Asp Ala Met Asp Gly Trp Phe Arg Leu

130 135 140

Glu Arg Val Ser Asp Asp Glu Phe Asn Asn Tyr Lys Leu Val Phe Cys

145 150 155 160

Pro Gln Gln Ala Glu Asp Asp Lys Cys Gly Asp Ile Gly Ile Ser Ile

165 170 175

Asp His Asp Asp Gly Thr Arg Arg Leu Val Val Ser Lys Asn Lys Pro

180 185 190

Leu Val Val Gln Phe Gln Lys Leu Asp Lys Glu Ser Leu Ala Lys Lys

195 200 205

Asn His Gly Leu Ser Arg Ser Glu

210 215

<210> 25

<211> 158

<212> PRT

<213> 人工序列

<220>

<223> Gly m 8 (2S albumin) NP_001238443 [MW= 18459.97 Da]

<400> 25

Met Thr Lys Phe Thr Ile Leu Leu Ile Ser Leu Leu Phe Cys Ile Ala

1 5 10 15

His Thr Cys Ser Ala Ser Lys Trp Gln His Gln Gln Asp Ser Cys Arg

20 25 30

Lys Gln Leu Gln Gly Val Asn Leu Thr Pro Cys Glu Lys His Ile Met

35 40 45

Glu Lys Ile Gln Gly Arg Gly Asp Asp Asp Asp Asp Asp Asp Asp Asp

50 55 60

Asn His Ile Leu Arg Thr Met Arg Gly Arg Ile Asn Tyr Ile Arg Arg

65 70 75 80

Asn Glu Gly Lys Asp Glu Asp Glu Glu Glu Glu Gly His Met Gln Lys

85 90 95

Cys Cys Thr Glu Met Ser Glu Leu Arg Ser Pro Lys Cys Gln Cys Lys

100 105 110

Ala Leu Gln Lys Ile Met Glu Asn Gln Ser Glu Glu Leu Glu Glu Lys

115 120 125

Gln Lys Lys Lys Met Glu Lys Glu Leu Ile Asn Leu Ala Thr Met Cys

130 135 140

Arg Phe Gly Pro Met Ile Gln Cys Asp Leu Ser Ser Asp Asp

145 150 155

<210> 26

<211> 280

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 ADC94422 [MW= 30186.22 Da]

<400> 26

Met Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Leu Ser Leu Ala Phe

1 5 10 15

Phe Leu Val Leu Leu Thr Lys Ala Asn Ser Thr Asn Thr Val Ser Phe

20 25 30

Thr Val Ser Lys Phe Ser Pro Arg Gln Gln Asn Leu Ile Phe Gln Gly

35 40 45

Asp Ala Ala Ile Ser Pro Ser Gly Val Leu Arg Leu Thr Lys Val Asp

50 55 60

Ser Ile Asp Val Pro Thr Thr Gly Ser Leu Gly Arg Ala Leu Tyr Ala

65 70 75 80

Thr Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp

85 90 95

Ala Thr Ser Phe Lys Phe Lys Val Phe Ser Pro Asn Lys Thr Ala Asp

100 105 110

Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Lys Pro Gln Ser Lys

115 120 125

Gly Gly Phe Leu Gly Leu Phe Asn Ser Asp Ser Lys Asn Lys Ser Val

130 135 140

Gln Thr Val Ala Val Glu Phe Asp Thr Tyr Tyr Asn Ala Lys Trp Asp

145 150 155 160

Pro Ala Asn Arg His Ile Gly Ile Asp Val Asn Ser Ile Lys Ser Val

165 170 175

Lys Thr Ala Ser Trp Gly Leu Ala Asn Gly Gln Ile Ala Gln Ile Leu

180 185 190

Ile Thr Tyr Asp Ala Asp Thr Ser Leu Leu Val Ala Ser Leu Ile His

195 200 205

Pro Ser Arg Lys Thr Ser Tyr Ile Leu Ser Glu Thr Val Ser Leu Lys

210 215 220

Ser Asn Leu Pro Glu Trp Val Asn Ile Gly Phe Ser Ala Thr Thr Gly

225 230 235 240

Leu Asn Lys Gly Phe Val Glu Thr His Asp Val Phe Ser Trp Ser Phe

245 250 255

Ala Ser Lys Leu Ser Asp Gly Ser Thr Ser Asp Thr Leu Asp Leu Pro

260 265 270

Ser Phe Leu Leu Asn Glu Ala Ile

275 280

<210> 27

<211> 864

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 CAA39604 [MW= 96817.14 Da]

<400> 27

Met Phe Gly Ile Phe Asp Lys Gly Gln Lys Ile Lys Gly Thr Val Val

1 5 10 15

Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser Ile Gly

20 25 30

Lys Gly Gly Val Ile Asp Thr Ala Thr Gly Ile Leu Gly Gln Gly Val

35 40 45

Ser Leu Val Gly Gly Val Ile Asp Thr Ala Thr Ser Phe Leu Gly Arg

50 55 60

Asn Ile Ser Met Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly Ser Gly

65 70 75 80

Asn Gly Lys Val Gly Lys Glu Val Tyr Leu Glu Lys His Leu Pro Thr

85 90 95

Leu Pro Thr Leu Gly Ala Arg Gln Asp Ala Phe Ser Ile Phe Phe Glu

100 105 110

Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys Asn Phe

115 120 125

Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp Ile Pro

130 135 140

Asn His Gly Thr Ile Glu Phe Val Cys Asn Ser Trp Val Tyr Asn Phe

145 150 155 160

Arg Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr Tyr Leu

165 170 175

Pro Ser Ala Thr Pro Ala Pro Leu Leu Lys Tyr Arg Lys Glu Glu Leu

180 185 190

Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Lys Asp Phe Asp Arg

195 200 205

Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Gly Gly

210 215 220

Asp Pro Arg Pro Ile Leu Gly Gly Ser Ser Ile Tyr Pro Tyr Pro Arg

225 230 235 240

Arg Val Arg Thr Gly Arg Glu Arg Thr Arg Thr Asp Pro Asn Ser Glu

245 250 255

Lys Pro Gly Glu Val Tyr Val Pro Arg Asp Glu Asn Phe Gly His Leu

260 265 270

Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser His Asp

275 280 285

Val Ile Pro Leu Phe Lys Ser Ala Ile Phe Gln Leu Arg Val Thr Ser

290 295 300

Ser Glu Phe Glu Ser Phe Glu Asp Val Arg Ser Leu Tyr Glu Gly Gly

305 310 315 320

Ile Lys Leu Pro Thr Asp Ile Leu Ser Gln Ile Ser Pro Leu Pro Ala

325 330 335

Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Val Leu Gln Phe Pro

340 345 350

Pro Pro His Val Ala Lys Val Ser Lys Ser Gly Trp Met Thr Asp Glu

355 360 365

Glu Phe Ala Arg Glu Val Ile Ala Gly Val Asn Pro Asn Val Ile Arg

370 375 380

Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Thr Leu Tyr

385 390 395 400

Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu Gln Leu Glu Ile Asn Met

405 410 415

Gly Gly Val Thr Val Glu Glu Ala Leu Ser Thr Gln Arg Leu Phe Ile

420 425 430

Leu Asp Tyr Gln Asp Ala Phe Ile Pro Tyr Leu Thr Arg Ile Asn Ser

435 440 445

Leu Pro Thr Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe Leu Lys

450 455 460

Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys Pro His

465 470 475 480

Pro Asp Gly Asp Asn Leu Gly Pro Glu Ser Ile Val Val Leu Pro Ala

485 490 495

Thr Glu Gly Val Asp Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val

500 505 510

Ile Val Asn Asp Ser Gly Tyr His Gln Leu Val Ser His Trp Leu Asn

515 520 525

Thr His Ala Val Met Glu Pro Phe Ala Ile Ala Thr Asn Arg His Leu

530 535 540

Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His Tyr Arg Asp

545 550 555 560

Thr Ile Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala Asp

565 570 575

Gly Ile Ile Glu Lys Ser Phe Leu Pro Gly Lys Tyr Ser Ile Glu Met

580 585 590

Ser Ser Ser Val Tyr Lys Asn Trp Val Phe Thr Asp Gln Ala Leu Pro

595 600 605

Ala Asp Leu Val Lys Arg Gly Leu Ala Ile Glu Asp Pro Ser Ala Pro

610 615 620

His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly

625 630 635 640

Leu Glu Ile Trp Asp Ala Ile Lys Thr Trp Val His Glu Tyr Val Ser

645 650 655

Leu Tyr Tyr Pro Thr Asp Ala Ala Val Gln Gln Asp Thr Glu Leu Gln

660 665 670

Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu Lys Glu

675 680 685

Lys Pro Trp Trp Pro Lys Met Gln Thr Thr Glu Asp Leu Ile Gln Ser

690 695 700

Cys Ser Ile Ile Val Trp Thr Ala Ser Ala Leu His Ala Ala Val Asn

705 710 715 720

Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn Arg Pro Thr Leu

725 730 735

Ala Arg Arg Phe Ile Pro Ala Glu Gly Thr Pro Glu Tyr Asp Glu Met

740 745 750

Val Lys Asn Pro Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe

755 760 765

Glu Thr Leu Ile Asp Leu Ser Val Ile Glu Ile Leu Ser Arg His Ala

770 775 780

Ser Asp Glu Ile Tyr Leu Gly Glu Arg Glu Thr Pro Asn Trp Thr Thr

785 790 795 800

Asp Lys Lys Ala Leu Glu Ala Phe Lys Arg Phe Gly Ser Lys Leu Thr

805 810 815

Gly Ile Glu Gly Lys Ile Asn Ala Arg Asn Ser Asp Pro Ser Leu Arg

820 825 830

Asn Arg Thr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu His Arg Ser

835 840 845

Ser Glu Glu Gly Leu Thr Phe Lys Gly Ile Pro Asn Ser Ile Ser Ile

850 855 860

<210> 28

<211> 449

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K共有序列

<400> 28

Val Leu Cys His Gly Val Ala Thr Thr Thr Met Ala Phe His Asp Asp

1 5 10 15

Glu Gly Gly Asp Lys Lys Ser Pro Lys Ser Leu Phe Leu Met Ser Asn

20 25 30

Ser Thr Arg Val Phe Lys Thr Asp Ala Gly Glu Met Arg Val Leu Lys

35 40 45

Ser His Gly Gly Arg Ile Phe Tyr Arg His Met His Ile Gly Phe Ile

50 55 60

Ser Met Glu Pro Lys Ser Leu Phe Val Pro Gln Tyr Leu Asp Ser Asn

65 70 75 80

Leu Ile Ile Phe Ile Arg Arg Gly Glu Ala Lys Leu Gly Phe Ile Tyr

85 90 95

Asp Asp Glu Leu Ala Glu Arg Arg Leu Lys Thr Gly Asp Leu Tyr Met

100 105 110

Ile Pro Ser Gly Ser Ala Phe Tyr Leu Val Asn Ile Gly Glu Gly Gln

115 120 125

Arg Leu His Val Ile Cys Ser Ile Asp Pro Ser Thr Ser Leu Gly Leu

130 135 140

Glu Thr Phe Gln Ser Phe Tyr Ile Gly Gly Gly Ala Asn Ser His Ser

145 150 155 160

Val Leu Ser Gly Phe Glu Pro Ala Ile Leu Glu Thr Ala Phe Asn Glu

165 170 175

Ser Arg Thr Val Val Glu Glu Ile Phe Ser Lys Glu Leu Asp Gly Pro

180 185 190

Ile Met Phe Val Asp Asp Ser His Ala Pro Ser Leu Trp Thr Lys Phe

195 200 205

Leu Gln Leu Lys Lys Asp Asp Lys Glu Gln Gln Leu Lys Lys Met Met

210 215 220

Gln Asp Gln Glu Glu Asp Glu Glu Glu Lys Gln Thr Ser Arg Ser Trp

225 230 235 240

Arg Lys Leu Leu Glu Thr Val Phe Gly Lys Val Asn Glu Lys Ile Glu

245 250 255

Asn Lys Asp Thr Ala Gly Ser Pro Ala Ser Tyr Asn Leu Tyr Asp Asp

260 265 270

Lys Lys Ala Asp Phe Lys Asn Ala Tyr Gly Trp Ser Lys Ala Leu His

275 280 285

Gly Gly Glu Tyr Pro Pro Leu Ser Glu Pro Asp Ile Gly Val Leu Leu

290 295 300

Val Lys Leu Ser Ala Gly Ser Met Leu Ala Pro His Val Asn Pro Ile

305 310 315 320

Ser Asp Glu Tyr Thr Ile Val Leu Ser Gly Tyr Gly Glu Leu His Ile

325 330 335

Gly Tyr Pro Asn Gly Ser Lys Ala Met Lys Thr Lys Ile Lys Gln Gly

340 345 350

Asp Val Phe Val Val Pro Arg Tyr Phe Pro Phe Cys Gln Val Ala Ser

355 360 365

Arg Asp Gly Pro Leu Glu Phe Phe Gly Phe Ser Thr Ser Ala Arg Lys

370 375 380

Asn Lys Pro Gln Phe Leu Ala Gly Ala Ala Ser Leu Leu Arg Thr Leu

385 390 395 400

Met Gly Pro Glu Leu Ser Ala Ala Phe Gly Val Ser Glu Asp Thr Leu

405 410 415

Arg Arg Ala Val Asp Ala Gln His Glu Ala Val Ile Leu Pro Ser Ala

420 425 430

Trp Ala Ala Pro Pro Glu Asn Ala Gly Lys Leu Lys Met Glu Glu Glu

435 440 445

Pro

<210> 29

<211> 473

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K Eric BAB21619 473aa

<400> 29

Lys Thr Thr Leu Leu Leu Leu Leu Phe Val Leu Cys His Gly Val Ala

1 5 10 15

Thr Thr Thr Met Ala Phe His Asp Asp Glu Gly Gly Asp Lys Lys Ser

20 25 30

Pro Lys Ser Leu Phe Leu Met Ser Asn Ser Thr Arg Val Phe Lys Thr

35 40 45

Asp Ala Gly Glu Met Arg Val Leu Lys Ser His Gly Gly Arg Ile Phe

50 55 60

Tyr Arg His Met His Ile Gly Phe Ile Ser Met Glu Pro Lys Ser Leu

65 70 75 80

Phe Val Pro Gln Tyr Leu Asp Ser Asn Leu Ile Ile Phe Ile Arg Arg

85 90 95

Gly Glu Ala Lys Leu Gly Phe Ile Tyr Asp Asp Glu Leu Ala Glu Arg

100 105 110

Arg Leu Lys Thr Gly Asp Leu Tyr Met Ile Pro Ser Gly Ser Ala Phe

115 120 125

Tyr Leu Val Asn Ile Gly Glu Gly Gln Arg Leu His Val Ile Cys Ser

130 135 140

Ile Asp Pro Ser Thr Ser Leu Gly Leu Glu Thr Phe Gln Ser Phe Tyr

145 150 155 160

Ile Gly Gly Gly Ala Asn Ser His Ser Val Leu Ser Gly Phe Glu Pro

165 170 175

Ala Ile Leu Glu Thr Ala Phe Asn Glu Ser Arg Thr Val Val Glu Glu

180 185 190

Ile Phe Ser Lys Glu Leu Asp Gly Pro Ile Met Phe Val Asp Asp Ser

195 200 205

His Ala Pro Ser Leu Trp Thr Lys Phe Leu Gln Leu Lys Lys Asp Asp

210 215 220

Lys Glu Gln Gln Leu Lys Lys Met Met Gln Asp Gln Glu Glu Asp Glu

225 230 235 240

Glu Glu Lys Gln Thr Ser Arg Ser Trp Arg Lys Leu Leu Glu Thr Val

245 250 255

Phe Gly Lys Val Asn Glu Lys Ile Glu Asn Lys Asp Thr Ala Gly Ser

260 265 270

Pro Ala Ser Tyr Asn Leu Tyr Asp Asp Lys Lys Ala Asp Phe Lys Asn

275 280 285

Ala Tyr Gly Trp Ser Lys Ala Leu His Gly Gly Glu Tyr Pro Pro Leu

290 295 300

Ser Glu Pro Asp Ile Gly Val Leu Leu Val Lys Leu Ser Ala Gly Ser

305 310 315 320

Met Leu Ala Pro His Val Asn Pro Ile Ser Asp Glu Tyr Thr Ile Val

325 330 335

Leu Ser Gly Tyr Gly Glu Leu His Ile Gly Tyr Pro Asn Gly Ser Arg

340 345 350

Ala Met Lys Thr Lys Ile Lys Gln Gly Asp Val Phe Val Val Pro Arg

355 360 365

Tyr Phe Pro Phe Cys Gln Val Ala Ser Arg Asp Gly Pro Leu Glu Phe

370 375 380

Phe Gly Phe Ser Thr Ser Ala Arg Lys Asn Lys Pro Gln Phe Leu Ala

385 390 395 400

Gly Ala Ala Ser Leu Leu Arg Thr Leu Met Gly Pro Glu Leu Ser Ala

405 410 415

Ala Phe Gly Val Ser Glu Asp Thr Leu Arg Arg Ala Val Asp Ala Gln

420 425 430

His Glu Ala Val Ile Leu Pro Ser Ala Trp Ala Ala Pro Pro Glu Asn

435 440 445

Ala Gly Lys Leu Lys Met Glu Glu Glu Pro Asn Ala Ile Arg Ser Phe

450 455 460

Ala Asn Asp Val Val Met Asp Val Phe

465 470

<210> 30

<211> 454

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K Ping ACD36978.1 455aa

<400> 30

Val Leu Cys His Gly Val Ala Thr Thr Thr Met Ala Phe His Asp Asp

1 5 10 15

Glu Gly Gly Asp Lys Lys Ser Pro Lys Ser Leu Phe Leu Met Ser Asn

20 25 30

Ser Thr Arg Val Phe Lys Thr Asp Ala Gly Glu Met Arg Val Leu Lys

35 40 45

Ser His Gly Gly Arg Ile Phe Tyr Arg His Met His Ile Gly Phe Ile

50 55 60

Ser Met Glu Pro Lys Ser Leu Phe Val Pro Gln Tyr Leu Asp Ser Asn

65 70 75 80

Leu Ile Ile Phe Ile Arg Arg Gly Glu Ala Lys Leu Gly Phe Ile Tyr

85 90 95

Asp Asp Glu Leu Ala Glu Arg Arg Leu Lys Thr Gly Asp Leu Tyr Met

100 105 110

Ile Pro Ser Gly Ser Ala Phe Tyr Leu Val Asn Ile Gly Glu Gly Gln

115 120 125

Arg Leu His Val Ile Cys Ser Ile Asp Pro Ser Thr Ser Leu Gly Leu

130 135 140

Glu Thr Phe Gln Ser Phe Tyr Ile Gly Gly Gly Ala Asn Ser His Ser

145 150 155 160

Val Leu Ser Gly Phe Glu Pro Ala Ile Leu Glu Thr Ala Phe Asn Glu

165 170 175

Ser Arg Thr Val Val Glu Glu Ile Phe Ser Lys Glu Leu Asp Gly Pro

180 185 190

Ile Met Phe Val Asp Asp Ser His Ala Pro Ser Leu Trp Thr Lys Phe

195 200 205

Leu Gln Leu Lys Lys Asp Asp Lys Glu Gln Gln Leu Lys Lys Met Met

210 215 220

Gln Asp Gln Glu Glu Asp Glu Glu Glu Lys Gln Thr Ser Arg Ser Trp

225 230 235 240

Arg Lys Leu Leu Glu Thr Val Phe Gly Lys Val Asn Glu Lys Ile Glu

245 250 255

Asn Lys Asp Thr Ala Gly Ser Pro Ala Ser Tyr Asn Leu Tyr Asp Asp

260 265 270

Lys Lys Ala Asp Phe Lys Asn Ala Tyr Gly Trp Ser Lys Ala Leu His

275 280 285

Gly Gly Glu Tyr Pro Pro Leu Ser Glu Pro Asp Ile Gly Val Leu Leu

290 295 300

Val Lys Leu Ser Ala Gly Ser Met Leu Ala Pro His Val Asn Pro Ile

305 310 315 320

Ser Asp Glu Tyr Thr Ile Val Leu Ser Gly Tyr Gly Glu Leu His Ile

325 330 335

Gly Pro Asn Gly Ser Lys Ala Met Lys Thr Lys Ile Lys Gln Gly Asp

340 345 350

Val Phe Val Val Pro Arg Tyr Phe Pro Phe Cys Gln Val Ala Ser Arg

355 360 365

Asp Gly Pro Leu Glu Phe Phe Gly Phe Ser Thr Ser Ala Arg Lys Asn

370 375 380

Lys Pro Gln Phe Leu Ala Gly Ala Ala Ser Leu Leu Arg Thr Leu Met

385 390 395 400

Gly Pro Glu Leu Ser Ala Ala Phe Gly Val Ser Glu Asp Thr Leu Arg

405 410 415

Arg Ala Val Asp Ala Gln His Glu Ala Val Ile Leu Pro Ser Ala Trp

420 425 430

Ala Ala Pro Arg Lys Met Gln Glu Ala Glu Met Glu Glu Ser Gln Met

435 440 445

Leu Leu Lys Leu Cys Gln

450

<210> 31

<211> 373

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K ACD36975.1 373aa

<400> 31

Leu Asp Ser Asn Leu Ile Ile Phe Ile Arg Arg Gly Glu Ala Lys Leu

1 5 10 15

Gly Phe Ile Tyr Asp Asp Glu Leu Ala Glu Arg Arg Leu Lys Thr Gly

20 25 30

Asp Leu Tyr Met Ile Pro Ser Gly Ser Ala Phe Tyr Leu Val Asn Ile

35 40 45

Gly Glu Gly Gln Arg Leu His Val Ile Cys Ser Ile Asp Pro Ser Thr

50 55 60

Ser Leu Gly Leu Glu Thr Phe Gln Ser Phe Tyr Ile Gly Gly Gly Ala

65 70 75 80

Asn Ser His Ser Val Leu Ser Gly Phe Glu Pro Ala Ile Leu Glu Thr

85 90 95

Ala Phe Asn Glu Ser Arg Thr Val Val Glu Glu Ile Phe Ser Lys Glu

100 105 110

Leu Asp Gly Pro Ile Met Phe Val Asp Asp Ser His Ala Pro Ser Leu

115 120 125

Trp Thr Lys Phe Leu Gln Leu Lys Lys Asp Asp Lys Glu Gln Gln Leu

130 135 140

Lys Lys Met Met Gln Asp Gln Glu Glu Asp Glu Glu Glu Lys Gln Thr

145 150 155 160

Ser Arg Ser Trp Arg Lys Leu Leu Glu Thr Val Phe Gly Lys Val Asn

165 170 175

Glu Lys Ile Glu Asn Lys Asp Thr Ala Gly Ser Pro Ala Ser Tyr Asn

180 185 190

Leu Tyr Asp Asp Lys Lys Ala Asp Phe Lys Asn Ala Tyr Gly Trp Ser

195 200 205

Lys Ala Leu His Gly Gly Glu Tyr Pro Pro Leu Ser Glu Pro Asp Ile

210 215 220

Gly Val Leu Leu Val Lys Leu Ser Ala Gly Ser Met Leu Ala Pro His

225 230 235 240

Val Asn Pro Ile Ser Asp Glu Tyr Thr Ile Val Leu Ser Gly Tyr Gly

245 250 255

Glu Leu His Ile Gly Tyr Pro Asn Gly Ser Lys Ala Met Lys Thr Lys

260 265 270

Ile Lys Gln Gly Asp Val Phe Val Val Pro Arg Tyr Phe Pro Phe Cys

275 280 285

Gln Val Ala Ser Arg Asp Gly Pro Leu Glu Phe Phe Gly Phe Ser Thr

290 295 300

Ser Ala Arg Lys Asn Lys Pro Gln Phe Leu Ala Gly Ala Ala Ser Leu

305 310 315 320

Leu Arg Thr Leu Met Gly Pro Glu Leu Ser Ala Ala Phe Gly Val Ser

325 330 335

Glu Asp Thr Leu Arg Arg Ala Val Asp Ala Gln His Glu Ala Val Ile

340 345 350

Leu Pro Ser Ala Trp Ala Ala Pro Pro Glu Asn Ala Gly Lys Leu Lys

355 360 365

Met Glu Glu Glu Pro

370

<210> 32

<211> 373

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K Ping ACD36976.1 373aa

<400> 32

Leu Asp Ser Asn Leu Ile Ile Phe Ile Arg Arg Gly Glu Ala Lys Leu

1 5 10 15

Gly Phe Ile Tyr Asp Asp Glu Leu Ala Glu Arg Arg Leu Lys Thr Gly

20 25 30

Asp Leu Tyr Met Ile Pro Ser Gly Ser Ala Phe Tyr Leu Val Asn Ile

35 40 45

Gly Glu Gly Gln Arg Leu His Val Ile Cys Ser Ile Asp Pro Ser Thr

50 55 60

Ser Leu Gly Leu Glu Thr Phe Gln Ser Phe Asn Ile Gly Gly Gly Ala

65 70 75 80

Asn Ser His Ser Val Leu Ser Gly Phe Glu Pro Ala Ile Leu Glu Thr

85 90 95

Ala Phe Asn Glu Ser Arg Thr Val Val Glu Glu Thr Phe Ser Lys Glu

100 105 110

Leu Asp Gly Pro Ile Met Phe Val Asp Asp Ser His Ala Pro Ser Leu

115 120 125

Trp Thr Lys Phe Leu Gln Leu Lys Lys Asp Asp Lys Glu Gln Gln Leu

130 135 140

Lys Lys Met Met Gln Asp Gln Glu Glu Asp Glu Glu Glu Lys Gln Thr

145 150 155 160

Ser Arg Ser Trp Arg Lys Leu Leu Glu Thr Val Phe Gly Lys Val Asn

165 170 175

Glu Lys Ile Glu Asn Lys Asp Thr Ala Gly Ser Pro Ala Ser Tyr Asn

180 185 190

Leu Tyr Asp Asp Lys Lys Ala Asp Phe Lys Asn Ala Tyr Gly Trp Ser

195 200 205

Lys Ala Leu His Gly Gly Glu Tyr Pro Pro Leu Ser Glu Pro Asp Ile

210 215 220

Gly Val Leu Leu Val Lys Leu Ser Ala Gly Ser Met Leu Ala Pro His

225 230 235 240

Val Asn Pro Ile Ser Asp Glu Tyr Thr Ile Val Leu Ser Gly Tyr Gly

245 250 255

Glu Leu His Ile Gly Tyr Pro Asn Gly Ser Lys Ala Met Lys Thr Lys

260 265 270

Ile Lys Gln Gly Asp Val Phe Val Val Pro Arg Tyr Phe Pro Phe Cys

275 280 285

Gln Val Ala Ser Arg Asp Gly Pro Leu Glu Phe Phe Gly Phe Ser Thr

290 295 300

Ser Ala Arg Lys Asn Lys Pro Gln Phe Leu Ala Gly Ala Ala Ser Leu

305 310 315 320

Leu Arg Thr Leu Met Gly Pro Glu Leu Ser Ala Ala Phe Gly Val Ser

325 330 335

Glu Asp Thr Leu Arg Arg Ala Val Asp Ala Gln His Ala Ala Val Ile

340 345 350

Leu Pro Ser Ala Trp Ala Ala Pro Pro Glu Asn Ala Gly Lys Leu Lys

355 360 365

Met Glu Glu Glu Pro

370

<210> 33

<211> 320

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 28 K Ping ACD36974.1 320aa

<400> 33

Leu Asp Ser Asn Leu Ile Ile Phe Ile Arg Arg Gly Glu Ala Lys Leu

1 5 10 15

Gly Phe Ile Tyr Asp Asp Glu Leu Ala Glu Arg Arg Leu Lys Thr Gly

20 25 30

Asp Leu Tyr Met Ile Pro Ser Gly Ser Ala Phe Tyr Leu Val Asn Ile

35 40 45

Gly Glu Gly Gln Arg Leu His Val Ile Cys Ser Ile Asp Pro Ser Thr

50 55 60

Ser Leu Gly Leu Glu Thr Phe Gln Ser Phe Tyr Ile Gly Gly Gly Ala

65 70 75 80

Asn Ser His Ser Val Leu Ser Gly Phe Glu Pro Ala Ile Leu Glu Thr

85 90 95

Ala Phe Asn Glu Ser Arg Thr Val Val Glu Glu Ile Phe Ser Lys Glu

100 105 110

Leu Asp Gly Pro Ile Met Phe Val Asp Asp Ser His Val Pro Ser Leu

115 120 125

Trp Thr Lys Phe Leu Gln Leu Lys Lys Asp Asp Lys Glu Gln Gln Leu

130 135 140

Lys Lys Met Met Gln Asp Gln Glu Glu Asp Glu Glu Glu Lys Gln Thr

145 150 155 160

Ser Arg Ser Trp Arg Lys Leu Leu Glu Thr Val Phe Gly Lys Val Asn

165 170 175

Glu Lys Ile Glu Asn Lys Asp Thr Ala Gly Ser Pro Ala Ser Tyr Asn

180 185 190

Leu Tyr Asp Asp Lys Lys Ala Asp Phe Lys Asn Ala Tyr Gly Trp Ser

195 200 205

Lys Ala Leu His Gly Gly Glu Tyr Pro Pro Leu Ser Glu Pro Asp Ile

210 215 220

Gly Val Leu Leu Val Lys Leu Ser Ala Gly Ser Met Leu Ala Pro His

225 230 235 240

Val Asn Pro Ile Ser Asp Glu Tyr Thr Ile Val Leu Ser Gly Tyr Gly

245 250 255

Glu Leu His Ile Gly Tyr Pro Asn Gly Ser Lys Ala Met Lys Thr Lys

260 265 270

Ile Lys Gln Gly Asp Val Phe Val Val Pro Arg Tyr Phe Pro Phe Cys

275 280 285

Gln Val Ala Ser Arg Asp Gly Pro Leu Glu Phe Phe Gly Phe Ser Thr

290 295 300

Ser Ala Arg Lys Asn Lys Pro Gln Phe Leu Ala Gly Ala Ala Ser Leu

305 310 315 320

<210> 34

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 34

Leu Gly Phe Ile Tyr Asp Asp Glu Leu Ala Glu Arg

1 5 10

<210> 35

<211> 9

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 35

Thr Val Val Glu Glu Ile Phe Ser Lys

1 5

<210> 36

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 36

Met Met Gln Asp Gln Glu Glu Asp Glu Glu Glu Lys

1 5 10

<210> 37

<211> 7

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 37

Asn Ala Tyr Gly Trp Ser Lys

1 5

<210> 38

<211> 21

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 38

Ala Leu His Gly Gly Glu Tyr Pro Pro Leu Ser Glu Pro Asp Ile Gly

1 5 10 15

Val Leu Leu Val Lys

20

<210> 39

<211> 9

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 39

Gln Gly Asp Val Phe Val Val Pro Arg

1 5

<210> 40

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 40

Tyr Phe Pro Phe Cys Gln Val Ala Ser Arg

1 5 10

<210> 41

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 41

Thr Leu Met Gly Pro Glu Leu Ser Ala Ala Phe Gly Val Ser Glu Asp

1 5 10 15

Thr Leu Arg

<210> 42

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 42

Ser Phe Ala Asn Asp Val Val Met Asp Val Phe

1 5 10

<210> 43

<211> 379

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 30 K Ping AAB09252.1 379aa (also serves as consensus

sequence)

<400> 43

Met Gly Phe Leu Val Leu Leu Leu Phe Ser Leu Leu Gly Leu Ser Ser

1 5 10 15

Ser Ser Ser Ile Ser Thr His Arg Ser Ile Leu Asp Leu Asp Leu Thr

20 25 30

Lys Phe Thr Thr Gln Lys Gln Val Ser Ser Leu Phe Gln Leu Trp Lys

35 40 45

Ser Glu His Gly Arg Val Tyr His Asn His Glu Glu Glu Ala Lys Arg

50 55 60

Leu Glu Ile Phe Lys Asn Asn Ser Asn Tyr Ile Arg Asp Met Asn Ala

65 70 75 80

Asn Arg Lys Ser Pro His Ser His Arg Leu Gly Leu Asn Lys Phe Ala

85 90 95

Asp Ile Thr Pro Gln Glu Phe Ser Lys Lys Tyr Leu Gln Ala Pro Lys

100 105 110

Asp Val Ser Gln Gln Ile Lys Met Ala Asn Lys Lys Met Lys Lys Glu

115 120 125

Gln Tyr Ser Cys Asp His Pro Pro Ala Ser Trp Asp Trp Arg Lys Lys

130 135 140

Gly Val Ile Thr Gln Val Lys Tyr Gln Gly Gly Cys Gly Arg Gly Trp

145 150 155 160

Ala Phe Ser Ala Thr Gly Ala Ile Glu Ala Ala His Ala Ile Ala Thr

165 170 175

Gly Asp Leu Val Ser Leu Ser Glu Gln Glu Leu Val Asp Cys Val Glu

180 185 190

Glu Ser Glu Gly Ser Tyr Asn Gly Trp Gln Tyr Gln Ser Phe Glu Trp

195 200 205

Val Leu Glu His Gly Gly Ile Ala Thr Asp Asp Asp Tyr Pro Tyr Arg

210 215 220

Ala Lys Glu Gly Arg Cys Lys Ala Asn Lys Ile Gln Asp Lys Val Thr

225 230 235 240

Ile Asp Gly Tyr Glu Thr Leu Ile Met Ser Asp Glu Ser Thr Glu Ser

245 250 255

Glu Thr Glu Gln Ala Phe Leu Ser Ala Ile Leu Glu Gln Pro Ile Ser

260 265 270

Val Ser Ile Asp Ala Lys Asp Phe His Leu Tyr Thr Gly Gly Ile Tyr

275 280 285

Asp Gly Glu Asn Cys Thr Ser Pro Tyr Gly Ile Asn His Phe Val Leu

290 295 300

Leu Val Gly Tyr Gly Ser Ala Asp Gly Val Asp Tyr Trp Ile Ala Lys

305 310 315 320

Asn Ser Trp Gly Glu Asp Trp Gly Glu Asp Gly Tyr Ile Trp Ile Gln

325 330 335

Arg Asn Thr Gly Asn Leu Leu Gly Val Cys Gly Met Asn Tyr Phe Ala

340 345 350

Ser Tyr Pro Thr Lys Glu Glu Ser Glu Thr Leu Val Ser Ala Arg Val

355 360 365

Lys Gly His Arg Arg Val Asp His Ser Pro Leu

370 375

<210> 44

<211> 379

<212> PRT

<213> 人工序列

<220>

<223> Gly m Bd 30 K Ping P22895.1 379aa

<400> 44

Met Gly Phe Leu Val Leu Leu Leu Phe Ser Leu Leu Gly Leu Ser Ser

1 5 10 15

Ser Ser Ser Ile Ser Thr His Arg Ser Ile Leu Asp Leu Asp Leu Thr

20 25 30

Lys Phe Thr Thr Gln Lys Gln Val Ser Ser Leu Phe Gln Leu Trp Lys

35 40 45

Ser Glu His Gly Arg Val Tyr His Asn His Glu Glu Glu Ala Lys Arg

50 55 60

Leu Glu Ile Phe Lys Asn Asn Ser Asn Tyr Ile Arg Asp Met Asn Ala

65 70 75 80

Asn Arg Lys Ser Pro His Ser His Arg Leu Gly Leu Asn Lys Phe Ala

85 90 95

Asp Ile Thr Pro Gln Glu Phe Ser Lys Lys Tyr Leu Gln Ala Pro Lys

100 105 110

Asp Val Ser Gln Gln Ile Lys Met Ala Asn Lys Lys Met Lys Lys Glu

115 120 125

Gln Tyr Ser Cys Asp His Pro Pro Ala Ser Trp Asp Trp Arg Lys Lys

130 135 140

Gly Val Ile Thr Gln Val Lys Tyr Gln Gly Gly Cys Gly Arg Gly Trp

145 150 155 160

Ala Phe Ser Ala Thr Gly Ala Ile Glu Ala Ala His Ala Ile Ala Thr

165 170 175

Gly Asp Leu Val Ser Leu Ser Glu Gln Glu Leu Val Asp Cys Val Glu

180 185 190

Glu Ser Glu Gly Ser Tyr Asn Gly Trp Gln Tyr Gln Ser Phe Glu Trp

195 200 205

Val Leu Glu His Gly Gly Ile Ala Thr Asp Asp Asp Tyr Pro Tyr Arg

210 215 220

Ala Lys Glu Gly Arg Cys Lys Ala Asn Lys Ile Gln Asp Lys Val Thr

225 230 235 240

Ile Asp Gly Tyr Glu Thr Leu Ile Met Ser Asp Glu Ser Thr Glu Ser

245 250 255

Glu Thr Glu Gln Ala Phe Leu Ser Ala Ile Leu Glu Gln Pro Ile Ser

260 265 270

Val Ser Ile Asp Ala Lys Asp Phe His Leu Tyr Thr Gly Gly Ile Tyr

275 280 285

Asp Gly Glu Asn Cys Thr Ser Pro Tyr Gly Ile Asn His Phe Val Leu

290 295 300

Leu Val Gly Tyr Gly Ser Ala Asp Gly Val Asp Tyr Trp Ile Ala Lys

305 310 315 320

Asn Ser Trp Gly Phe Asp Trp Gly Glu Asp Gly Tyr Ile Trp Ile Gln

325 330 335

Arg Asn Thr Gly Asn Leu Leu Gly Val Cys Gly Met Asn Tyr Phe Ala

340 345 350

Ser Tyr Pro Thr Lys Glu Glu Ser Glu Thr Leu Val Ser Ala Arg Val

355 360 365

Lys Gly His Arg Arg Val Asp His Ser Pro Leu

370 375

<210> 45

<211> 9

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 45

Ser Ile Leu Asp Leu Asp Leu Thr Lys

1 5

<210> 46

<211> 5

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 46

Phe Thr Thr Gln Lys

1 5

<210> 47

<211> 7

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 47

Asn Asn Leu Asn Tyr Ile Arg

1 5

<210> 48

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 48

Phe Ala Asp Ile Thr Pro Gln Glu Phe Ser Lys

1 5 10

<210> 49

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 49

Glu Gln Tyr Ser Cys Asp His Pro Pro Ala Ser Trp Asp Trp Arg

1 5 10 15

<210> 50

<211> 40

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 50

Val Thr Ile Asp Gly Tyr Glu Thr Leu Ile Met Ser Asp Glu Ser Thr

1 5 10 15

Glu Ser Glu Thr Glu Gln Ala Phe Leu Ser Ala Ile Leu Glu Gln Pro

20 25 30

Ile Ser Val Ser Ile Asp Ala Lys

35 40

<210> 51

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 51

Asn Thr Gly Asn Leu Leu Gly Val Cys Gly Met Asn Tyr Phe Ala Ser

1 5 10 15

Tyr Pro Thr Lys

20

<210> 52

<211> 203

<212> PRT

<213> 人工序列

<220>

<223> KTI 1共有序列

<400> 52

Met Lys Ser Thr Ile Phe Phe Ala Leu Phe Leu Val Cys Ala Phe Thr

1 5 10 15

Ile Ser Tyr Leu Pro Ser Ala Thr Ala Gln Phe Val Leu Asp Thr Asp

20 25 30

Asp Asp Pro Leu Gln Asn Gly Gly Thr Tyr Tyr Met Leu Pro Val Met

35 40 45

Arg Gly Lys Gly Gly Gly Ile Glu Gly Ala Ser Thr Gly Lys Glu Ile

50 55 60

Cys Pro Leu Thr Val Val Gln Ser Pro Asn Glu Leu Asp Lys Gly Ile

65 70 75 80

Gly Leu Val Phe Ser Ser Pro Leu His Ala Leu Phe Ile Ala Glu Arg

85 90 95

Tyr Pro Leu Ser Ile Lys Phe Gly Ser Phe Ala Val Ile Ser Leu Cys

100 105 110

Gly Gly Met Pro Thr Lys Trp Ala Ile Val Glu Arg Glu Gly Leu Gln

115 120 125

Ala Val Thr Leu Ala Ala Arg Asp Thr Val Asp Gly Trp Phe Asn Ile

130 135 140

Glu Arg Val Ser Arg Glu Tyr Asn Asp Tyr Lys Leu Val Phe Cys Pro

145 150 155 160

Gln Asn Ala Glu Asp Asn Lys Cys Glu Asp Ile Gly Ile Gln Ile Asp

165 170 175

Asn Asp Gly Ile Arg Arg Leu Val Leu Ser Lys Asn Lys Pro Leu Val

180 185 190

Val Gln Phe Gln Lys Phe Arg Ser Ser Thr Ala

195 200

<210> 53

<211> 204

<212> PRT

<213> 人工序列

<220>

<223> KTI 1 AAB23483.1 204aa

<400> 53

Met Lys Ser Thr Ile Phe Phe Ala Leu Phe Leu Val Cys Ala Phe Thr

1 5 10 15

Ile Ser Tyr Leu Pro Ser Ala Thr Ala Gln Phe Val Leu Asp Thr Asp

20 25 30

Asp Asp Pro Leu Gln Asn Gly Gly Thr Tyr Tyr Met Leu Pro Val Met

35 40 45

Arg Gly Lys Ser Gly Gly Ile Glu Gly Asn Ser Thr Gly Lys Glu Ile

50 55 60

Cys Pro Leu Thr Val Val Gln Ser Pro Asn Lys His Asn Lys Gly Ile

65 70 75 80

Gly Leu Val Phe Lys Ser Pro Leu His Ala Leu Phe Ile Ala Glu Arg

85 90 95

Tyr Pro Leu Ser Ile Lys Phe Asp Ser Phe Ala Val Ile Pro Leu Cys

100 105 110

Gly Val Met Pro Thr Lys Trp Ala Ile Val Glu Arg Glu Gly Leu Gln

115 120 125

Ala Val Thr Leu Ala Ala Arg Asp Thr Val Asp Gly Trp Phe Asn Ile

130 135 140

Glu Arg Val Ser Arg Glu Tyr Asn Asp Tyr Tyr Lys Leu Val Phe Cys

145 150 155 160

Pro Gln Glu Ala Glu Asp Asn Lys Cys Glu Asp Ile Gly Ile Gln Ile

165 170 175

Asp Asn Asp Gly Ile Arg Arg Leu Val Leu Ser Lys Asn Lys Pro Leu

180 185 190

Val Val Glu Phe Gln Lys Phe Arg Ser Ser Thr Ala

195 200

<210> 54

<211> 208

<212> PRT

<213> 人工序列

<220>

<223> KTI 1 CAA56343.1 208aa

<400> 54

Met Lys Ser Thr Thr Ser Leu Ala Leu Phe Leu Leu Cys Ala Leu Thr

1 5 10 15

Ser Ser Tyr Gln Pro Ser Ala Thr Ala Asp Ile Val Phe Asp Thr Glu

20 25 30

Gly Asn Pro Ile Arg Asn Gly Gly Thr Tyr Tyr Val Leu Pro Val Ile

35 40 45

Arg Gly Lys Gly Gly Gly Ile Glu Phe Ala Lys Thr Glu Thr Glu Thr

50 55 60

Cys Pro Leu Thr Val Val Gln Ser Pro Phe Glu Gly Leu Gln Arg Gly

65 70 75 80

Leu Pro Leu Ile Ile Ser Ser Pro Phe Lys Ile Leu Asp Ile Thr Glu

85 90 95

Gly Leu Ile Leu Ser Leu Lys Phe His Leu Cys Thr Pro Leu Ser Leu

100 105 110

Asn Ser Phe Ser Val Asp Arg Tyr Ser Gln Gly Ser Ala Arg Arg Thr

115 120 125

Pro Cys Gln Thr His Trp Leu Gln Lys His Asn Arg Cys Trp Phe Arg

130 135 140

Ile Gln Arg Ala Ser Ser Glu Ser Asn Tyr Tyr Lys Leu Val Phe Cys

145 150 155 160

Thr Ser Asn Asp Asp Ser Ser Cys Gly Asp Ile Val Ala Pro Ile Asp

165 170 175

Arg Glu Gly Asn Arg Pro Leu Ile Val Thr His Asp Gln Asn His Pro

180 185 190

Leu Leu Val Gln Phe Gln Lys Val Glu Ala Tyr Glu Ser Ser Thr Ala

195 200 205

<210> 55

<211> 16

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 55

Glu Ile Cys Pro Leu Thr Val Val Gln Ser Pro Asn Glu Leu Asp Lys

1 5 10 15

<210> 56

<211> 7

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 56

Glu Gly Leu Gln Ala Val Lys

1 5

<210> 57

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 57

Leu Val Phe Cys Pro Gln Gln Ala Glu Asp Asn Lys

1 5 10

<210> 58

<211> 14

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 58

Cys Glu Asp Ile Gly Ile Gln Ile Asp Asp Asp Gly Ile Arg

1 5 10

<210> 59

<211> 5

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 59

Leu Val Leu Ser Lys

1 5

<210> 60

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 60

Asn Lys Pro Leu Val Val Gln Phe Gln Lys

1 5 10

<210> 61

<211> 217

<212> PRT

<213> 人工序列

<220>

<223> KTI 3共有序列

<400> 61

Met Lys Ser Thr Ile Phe Phe Ala Leu Phe Leu Phe Cys Ala Phe Thr

1 5 10 15

Thr Ser Tyr Leu Pro Ser Ala Ile Ala Asp Phe Val Leu Asp Asn Glu

20 25 30

Gly Asn Pro Leu Glu Asn Gly Gly Thr Tyr Tyr Ile Leu Ser Asp Ile

35 40 45

Thr Ala Phe Gly Gly Ile Arg Ala Ala Pro Thr Gly Asn Glu Arg Cys

50 55 60

Pro Leu Thr Val Val Gln Ser Arg Asn Glu Leu Asp Lys Gly Ile Gly

65 70 75 80

Thr Ile Ile Ser Ser Pro Tyr Arg Ile Arg Phe Ile Ala Glu Gly His

85 90 95

Pro Leu Ser Leu Lys Phe Asp Ser Phe Ala Val Ile Met Leu Cys Val

100 105 110

Gly Ile Pro Thr Glu Trp Ser Val Val Glu Asp Leu Pro Glu Gly Pro

115 120 125

Ala Val Lys Ile Gly Glu Asn Lys Asp Ala Met Asp Gly Trp Phe Arg

130 135 140

Leu Glu Arg Val Ser Asp Asp Glu Phe Asn Asn Tyr Lys Leu Val Phe

145 150 155 160

Cys Pro Gln Gln Ala Glu Asp Asp Lys Cys Gly Asp Ile Gly Ile Ser

165 170 175

Ile Asp His Asp Asp Gly Thr Arg Arg Leu Val Val Ser Lys Asn Lys

180 185 190

Pro Leu Val Val Gln Phe Gln Lys Leu Asp Lys Glu Ser Leu Ala Lys

195 200 205

Lys Asn His Gly Leu Ser Arg Ser Glu

210 215

<210> 62

<211> 217

<212> PRT

<213> 人工序列

<220>

<223> KTI 3 CAA45777.1 217aa

<400> 62

Met Lys Ser Thr Ile Phe Phe Ala Leu Phe Leu Phe Cys Ala Phe Thr

1 5 10 15

Thr Ser Tyr Leu Pro Ser Ala Ile Ala Asp Phe Val Leu Asp Asn Glu

20 25 30

Gly Asn Pro Leu Glu Asn Gly Gly Thr Tyr Tyr Ile Leu Ser Asp Ile

35 40 45

Thr Ala Phe Gly Gly Ile Arg Ala Ala Pro Thr Gly Asn Glu Arg Cys

50 55 60

Pro Leu Thr Val Val Gln Ser Arg Asn Glu Leu Asp Lys Gly Ile Gly

65 70 75 80

Thr Ile Ile Ser Ser Pro Tyr Arg Ile Arg Phe Ile Ala Glu Gly His

85 90 95

Pro Leu Ser Leu Lys Phe Asp Ser Phe Ala Val Ile Met Leu Cys Val

100 105 110

Gly Ile Pro Thr Glu Trp Ser Val Val Glu Asp Leu Pro Glu Gly Pro

115 120 125

Ala Val Lys Ile Gly Glu Asn Lys Asp Ala Met Asp Gly Trp Phe Arg

130 135 140

Leu Glu Arg Val Ser Asp Asp Glu Phe Asn Asn Tyr Lys Leu Val Phe

145 150 155 160

Cys Pro Gln Gln Ala Glu Asp Asp Lys Cys Gly Asp Ile Gly Ile Ser

165 170 175

Ile Asp His Asp Asp Gly Thr Arg Arg Leu Val Val Ser Lys Asn Lys

180 185 190

Pro Leu Val Val Gln Phe Gln Lys Leu Asp Lys Glu Ser Leu Ala Lys

195 200 205

Lys Asn His Gly Leu Ser Arg Ser Glu

210 215

<210> 63

<211> 217

<212> PRT

<213> 人工序列

<220>

<223> KTI 3 CAA45778.1 217aa

<400> 63

Met Lys Ser Thr Ile Phe Phe Ala Leu Phe Leu Phe Cys Ala Phe Thr

1 5 10 15

Thr Ser Tyr Leu Pro Ser Ala Ile Ala Asp Phe Val Leu Asp Asn Glu

20 25 30

Gly Asn Pro Leu Asp Ser Gly Gly Thr Tyr Tyr Ile Leu Ser Asp Ile

35 40 45

Thr Ala Phe Gly Gly Ile Arg Ala Ala Pro Thr Gly Asn Glu Arg Cys

50 55 60

Pro Leu Thr Val Val Gln Ser Arg Asn Glu Leu Asp Lys Gly Ile Gly

65 70 75 80

Thr Ile Ile Ser Ser Pro Phe Arg Ile Arg Phe Ile Ala Glu Gly Asn

85 90 95

Pro Leu Arg Leu Lys Phe Asp Ser Phe Ala Val Ile Met Leu Cys Val

100 105 110

Gly Ile Pro Thr Glu Trp Ser Val Val Glu Asp Leu Pro Glu Gly Pro

115 120 125

Ala Val Lys Ile Gly Glu Asn Lys Asp Ala Val Asp Gly Trp Phe Arg

130 135 140

Ile Glu Arg Val Ser Asp Asp Glu Phe Asn Asn Tyr Lys Leu Val Phe

145 150 155 160

Cys Thr Gln Gln Ala Glu Asp Asp Lys Cys Gly Asp Ile Gly Ile Ser

165 170 175

Ile Asp His Asp Asp Gly Thr Arg Arg Leu Val Val Ser Lys Asn Lys

180 185 190

Pro Leu Val Val Gln Phe Gln Lys Val Asp Lys Glu Ser Leu Ala Lys

195 200 205

Lys Asn His Gly Leu Ser Arg Ser Glu

210 215

<210> 64

<211> 9

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 64

Cys Pro Leu Thr Val Val Gln Ser Arg

1 5

<210> 65

<211> 5

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 65

Asn Glu Leu Asp Lys

1 5

<210> 66

<211> 5

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 66

Ile Gly Glu Asn Lys

1 5

<210> 67

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 67

Asp Ala Met Asp Gly Trp Phe Arg

1 5

<210> 68

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 68

Leu Val Phe Cys Pro Gln Gln Ala Glu Asp Asp Lys

1 5 10

<210> 69

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 69

Cys Gly Asp Ile Gly Ile Ser Ile Asp His Asp Asp Gly Thr Arg

1 5 10 15

<210> 70

<211> 5

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 70

Leu Val Val Ser Lys

1 5

<210> 71

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 71

Asn Lys Pro Leu Val Val Gln Phe Gln Lys

1 5 10

<210> 72

<211> 173

<212> PRT

<213> 人工序列

<220>

<223> 2S白蛋白Glyma13g36400.1 BLAST 174aa

<400> 72

Met Pro Pro Pro Ser Leu His Phe Thr Ser Pro Ile Asn Ser Lys Met

1 5 10 15

Thr Lys Phe Thr Ile Leu Leu Ile Ser Leu Leu Phe Cys Ile Ala His

20 25 30

Thr Cys Ser Ala Ser Lys Trp Gln His Gln Gln Asp Ser Cys Arg Lys

35 40 45

Gln Leu Gln Gly Val Asn Leu Thr Pro Cys Glu Lys His Ile Met Glu

50 55 60

Lys Ile Gln Gly Arg Gly Asp Asp Asp Asp Asp Asp Asp Asp Asp Asn

65 70 75 80

His Ile Leu Arg Thr Met Arg Gly Arg Ile Asn Tyr Ile Arg Arg Asn

85 90 95

Glu Gly Lys Asp Glu Asp Glu Glu Glu Glu Gly His Met Gln Lys Cys

100 105 110

Cys Thr Glu Met Ser Glu Leu Arg Ser Pro Lys Cys Gln Cys Lys Ala

115 120 125

Leu Gln Lys Ile Met Glu Asn Gln Ser Glu Glu Leu Glu Glu Lys Gln

130 135 140

Lys Lys Lys Met Glu Lys Glu Leu Ile Asn Leu Ala Thr Met Cys Arg

145 150 155 160

Phe Gly Pro Met Ile Gln Cys Asp Leu Ser Ser Asp Asp

165 170

<210> 73

<211> 158

<212> PRT

<213> 人工序列

<220>

<223> 2S白蛋白NP_001234950 BLAST 158aa

<400> 73

Met Thr Lys Phe Thr Ile Leu Leu Ile Ser Leu Leu Phe Cys Ile Ala

1 5 10 15

His Thr Cys Ser Ala Ser Glu Trp Gln His Gln Gln Asp Ser Cys Arg

20 25 30

Lys Gln Leu Gln Gly Val Asn Leu Thr Pro Cys Glu Lys His Ile Met

35 40 45

Glu Lys Ile Gln Gly Arg Gly Asp Asp Asp Asp Asp Asp Asp Asp Asp

50 55 60

Asn His Ile Leu Arg Thr Met Arg Gly Arg Ile Asn Tyr Ile Arg Arg

65 70 75 80

Asn Glu Gly Lys Asp Glu Asp Glu Glu Glu Glu Gly His Met Gln Lys

85 90 95

Cys Arg Thr Glu Met Ser Glu Leu Arg Ser Pro Lys Cys Gln Cys Lys

100 105 110

Ala Leu Gln Lys Ile Met Glu Asn Gln Ser Glu Glu Leu Glu Glu Lys

115 120 125

Gln Lys Lys Lys Met Glu Lys Glu Leu Ile Asn Leu Ala Thr Met Cys

130 135 140

Arg Phe Gly Pro Met Ile Gln Cys Asp Leu Ser Ser Asp Asp

145 150 155

<210> 74

<211> 155

<212> PRT

<213> 人工序列

<220>

<223> 2S白蛋白Glyma12g34160.1 BLAST 156aa

<400> 74

Met Thr Lys Leu Thr Ile Leu Leu Ile Ala Leu Leu Phe Ile Ala His

1 5 10 15

Thr Cys Cys Ala Ser Lys Trp Gln Gln His Gln Gln Glu Ser Cys Arg

20 25 30

Glu Gln Leu Lys Gly Ile Asn Leu Asn Pro Cys Glu His Ile Met Glu

35 40 45

Lys Ile Gln Ala Gly Arg Arg Gly Glu Asp Gly Ser Asp Glu Asp His

50 55 60

Ile Leu Ile Arg Thr Met Pro Gly Arg Ile Asn Tyr Ile Arg Lys Lys

65 70 75 80

Glu Gly Lys Glu Glu Glu Glu Glu Gly His Met Gln Lys Cys Cys Ser

85 90 95

Glu Met Ser Glu Leu Lys Ser Pro Ile Cys Gln Cys Lys Ala Leu Gln

100 105 110

Lys Ile Met Asp Asn Gln Ser Glu Gln Leu Glu Gly Lys Glu Lys Lys

115 120 125

Gln Met Glu Arg Glu Leu Met Asn Leu Ala Ile Arg Cys Arg Leu Gly

130 135 140

Pro Met Ile Gly Cys Asp Leu Ser Ser Asp Asp

145 150 155

<210> 75

<211> 9

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 75

Trp Gln His Gln Gln Asp Ser Cys Arg

1 5

<210> 76

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 76

Gln Leu Gln Gly Val Asn Leu Thr Pro Cys Glu Lys

1 5 10

<210> 77

<211> 5

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 77

His Ile Met Glu Lys

1 5

<210> 78

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 78

Asp Glu Asp Glu Glu Glu Glu Gly His Met Gln Lys

1 5 10

<210> 79

<211> 9

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 79

Cys Cys Thr Glu Met Ser Glu Leu Arg

1 5

<210> 80

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 80

Glu Leu Ile Asn Leu Ala Thr Met Cys Arg

1 5 10

<210> 81

<211> 13

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 81

Phe Gly Pro Met Ile Gln Cys Asp Leu Ser Ser Asp Asp

1 5 10

<210> 82

<211> 280

<212> PRT

<213> 人工序列

<220>

<223> 凝集素共有序列

<400> 82

Met Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Leu Ser Leu Ala Phe

1 5 10 15

Phe Leu Val Leu Leu Thr Lys Ala Asn Ser Thr Asn Thr Val Ser Phe

20 25 30

Thr Phe Ser Lys Phe Ser Pro Arg Gln Pro Asn Leu Ile Leu Gln Gly

35 40 45

Asp Ala Ala Ile Ser Ser Ser Gly Val Leu Arg Leu Thr Lys Val Asp

50 55 60

Ser Asn Gly Val Pro Thr Ser Gly Ser Leu Gly Arg Ala Leu Tyr Ala

65 70 75 80

Ala Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp

85 90 95

Ala Thr Ser Phe Lys Phe Asn Val Phe Ala Pro Asn Lys Thr Ala Asp

100 105 110

Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Lys Pro Gln Ser Lys

115 120 125

Gly Gly Phe Leu Gly Leu Phe Asn Ser Asp Ser Lys Asp Lys Ser Leu

130 135 140

Gln Thr Val Ala Val Glu Phe Asp Thr Tyr Ser Asn Lys Lys Trp Asp

145 150 155 160

Pro Ala Asn Arg His Ile Gly Ile Asp Val Asn Ser Ile Lys Ser Val

165 170 175

Lys Thr Ala Ser Trp Gly Leu Ala Asn Gly Gln Val Ala Gln Ile Leu

180 185 190

Ile Thr Tyr Asp Ala Ala Thr Ser Leu Leu Val Ala Ser Leu Ile His

195 200 205

Pro Ser Arg Lys Thr Ser Tyr Ile Leu Ser Glu Thr Val Ser Leu Lys

210 215 220

Ser Asn Leu Pro Glu Trp Val Ser Ile Gly Phe Ser Ala Thr Thr Gly

225 230 235 240

Leu Asn Glu Gly Ser Val Glu Thr His Asp Val Ile Ser Trp Ser Phe

245 250 255

Ala Ser Lys Leu Ser Asp Gly Ser Thr Ser Asp Ala Leu Asp Leu Pro

260 265 270

Ser Phe Leu Leu Asn Glu Ala Ile

275 280

<210> 83

<211> 280

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 XP_003535884 BLAST 280aa

<400> 83

Met Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Leu Ser Leu Ala Leu

1 5 10 15

Phe Leu Met Leu Leu Thr Lys Ala Asn Ser Thr Asn Thr Val Ser Phe

20 25 30

Thr Thr Ser Lys Phe Ser Pro Arg Gln Gln Asn Leu Ile Leu Gln Gly

35 40 45

Asp Ala Ala Ile Ser Pro Ser Gly Val Leu Arg Leu Thr Lys Val Asp

50 55 60

Ser Tyr Gly Val Pro Thr Ser Arg Ser Leu Gly Arg Ala Leu Tyr Ala

65 70 75 80

Ala Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp

85 90 95

Ala Thr Ser Phe Lys Phe Asn Val Phe Ser Pro Asp Lys Thr Ala Asp

100 105 110

Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Lys Pro Gln Tyr Lys

115 120 125

Ala Gly Phe Leu Gly Leu Phe Asn Ser Asp Ser Lys Asn Met Ser Leu

130 135 140

Gln Thr Val Ala Val Glu Phe Asp Thr Tyr Tyr Asn Gln Lys Trp Asp

145 150 155 160

Pro Ala Ser Arg His Ile Gly Ile Asp Val Asn Ser Ile Lys Ser Val

165 170 175

Lys Thr Ala Pro Trp Gly Phe Ala Asn Gly Gln Val Ala Gln Ile Leu

180 185 190

Ile Thr Tyr Asn Ala Asp Thr Ser Leu Leu Val Ala Ser Leu Val His

195 200 205

Pro Ser Arg Lys Thr Ser Tyr Ile Leu Ser Glu Thr Val Ser Leu Lys

210 215 220

Ser Asn Leu Pro Glu Trp Val Asn Val Gly Phe Ser Ala Thr Thr Gly

225 230 235 240

Ala Asn Lys Gly Phe Ala Glu Thr His Asp Val Phe Ser Trp Ser Phe

245 250 255

Ala Ser Lys Leu Ser Asp Gly Ser Thr Ser Asp Thr Leu Asp Leu Ala

260 265 270

Ser Phe Leu Leu Asn Glu Ala Ile

275 280

<210> 84

<211> 270

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 Glyma10g15480.1 BLAST

<400> 84

Met Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Leu Ser Leu Ala Leu

1 5 10 15

Phe Leu Met Leu Leu Thr Lys Ala Asn Ser Thr Asn Thr Val Ser Phe

20 25 30

Thr Thr Ser Lys Phe Ser Pro Arg Gln Gln Asn Leu Ile Leu Gln Gly

35 40 45

Asp Ala Ala Ile Ser Pro Ser Gly Val Leu Arg Leu Thr Lys Val Asp

50 55 60

Ser Tyr Gly Val Pro Thr Ser Arg Ser Leu Gly Arg Ala Leu Tyr Ala

65 70 75 80

Ala Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp

85 90 95

Ala Thr Ser Phe Lys Phe Asn Val Phe Ser Pro Asp Lys Thr Ala Asp

100 105 110

Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Lys Pro Gln Tyr Lys

115 120 125

Ala Gly Phe Leu Gly Leu Phe Asn Ser Asp Ser Lys Asn Met Ser Leu

130 135 140

Gln Thr Val Ala Trp Asp Pro Ala Ser Arg His Ile Gly Ile Asp Val

145 150 155 160

Asn Ser Ile Lys Ser Val Lys Thr Ala Pro Trp Gly Phe Ala Asn Gly

165 170 175

Gln Val Ala Gln Ile Leu Ile Thr Tyr Asn Ala Asp Thr Ser Leu Leu

180 185 190

Val Ala Ser Leu Val His Pro Ser Arg Lys Thr Ser Tyr Ile Leu Ser

195 200 205

Glu Thr Val Ser Leu Lys Ser Asn Leu Pro Glu Trp Val Asn Val Gly

210 215 220

Phe Ser Ala Thr Thr Gly Ala Asn Lys Gly Phe Ala Glu Thr His Asp

225 230 235 240

Val Phe Ser Trp Ser Phe Ala Ser Lys Leu Ser Asp Gly Ser Thr Ser

245 250 255

Asp Thr Leu Asp Leu Ala Ser Phe Leu Leu Asn Glu Ala Ile

260 265 270

<210> 85

<211> 282

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 NP_001237210 BLAST 282aa

<400> 85

Met Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Val Ser Leu Ala Phe

1 5 10 15

Phe Leu Val Leu Leu Thr Lys Ala His Ser Thr Asp Thr Val Ser Phe

20 25 30

Thr Phe Asn Lys Phe Asn Pro Val Gln Pro Asn Ile Met Leu Gln Lys

35 40 45

Asp Ala Ser Ile Ser Ser Ser Gly Val Leu Gln Leu Thr Lys Val Gly

50 55 60

Ser Asn Gly Val Pro Thr Ser Gly Ser Leu Gly Arg Ala Leu Tyr Ala

65 70 75 80

Ala Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp

85 90 95

Ala Thr Ser Phe Lys Phe Asn Ile Phe Ala Pro Asn Lys Ser Asn Ser

100 105 110

Ala Asp Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Gln Pro Gln

115 120 125

Ser Asp Asp Gly Phe Leu Gly Leu Phe Asn Ser Pro Leu Lys Asp Lys

130 135 140

Ser Leu Gln Thr Val Ala Ile Glu Phe Asp Thr Phe Ser Asn Lys Lys

145 150 155 160

Trp Asp Pro Ala Asn Arg His Ile Gly Ile Asp Val Asn Ser Ile Lys

165 170 175

Ser Val Lys Thr Ala Ser Trp Gly Leu Ser Asn Gly Gln Val Ala Glu

180 185 190

Ile Leu Val Thr Tyr Asn Ala Ala Thr Ser Leu Leu Val Ala Ser Leu

195 200 205

Ile His Pro Ser Lys Lys Thr Ser Tyr Ile Leu Ser Asp Thr Val Asn

210 215 220

Leu Lys Ser Asn Leu Pro Glu Trp Val Ser Val Gly Phe Ser Ala Thr

225 230 235 240

Thr Gly Leu His Glu Gly Ser Val Glu Thr His Asp Val Ile Ser Trp

245 250 255

Ser Phe Ala Ser Lys Leu Ser Asp Gly Ser Ser Asn Asp Ala Leu Asp

260 265 270

Leu Pro Ser Phe Val Leu Asn Glu Ala Ile

275 280

<210> 86

<211> 297

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 Glyma02g18090.1 BLAST

<400> 86

Met Lys Val Leu Cys Ile Ile Phe Glu Phe Lys Gln Ile Lys Ala Met

1 5 10 15

Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Val Ser Leu Ala Phe Phe

20 25 30

Leu Val Leu Leu Thr Lys Ala His Ser Thr Asp Thr Val Ser Phe Thr

35 40 45

Phe Asn Lys Phe Asn Pro Val Gln Pro Asn Ile Met Leu Gln Lys Asp

50 55 60

Ala Ser Ile Ser Ser Ser Gly Val Leu Gln Leu Thr Lys Val Gly Ser

65 70 75 80

Asn Gly Val Pro Thr Ser Gly Ser Leu Gly Arg Ala Leu Tyr Ala Ala

85 90 95

Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp Ala

100 105 110

Thr Ser Phe Lys Phe Asn Ile Phe Ala Pro Asn Lys Ser Asn Ser Ala

115 120 125

Asp Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Gln Pro Gln Ser

130 135 140

Asp Asp Gly Phe Leu Gly Leu Phe Asn Ser Pro Leu Lys Asp Lys Ser

145 150 155 160

Leu Gln Thr Val Ala Ile Glu Phe Asp Thr Phe Ser Asn Lys Lys Trp

165 170 175

Asp Pro Ala Asn Arg His Ile Gly Ile Asp Val Asn Ser Ile Lys Ser

180 185 190

Val Lys Thr Ala Ser Trp Gly Leu Ser Asn Gly Gln Val Ala Glu Ile

195 200 205

Leu Val Thr Tyr Asn Ala Ala Thr Ser Leu Leu Val Ala Ser Leu Ile

210 215 220

His Pro Ser Lys Lys Thr Ser Tyr Ile Leu Ser Asp Thr Val Asn Leu

225 230 235 240

Lys Ser Asn Leu Pro Glu Trp Val Ser Val Gly Phe Ser Ala Thr Thr

245 250 255

Gly Leu His Glu Gly Ser Val Glu Thr His Asp Val Ile Ser Trp Ser

260 265 270

Phe Ala Ser Lys Leu Ser Asp Gly Ser Ser Asn Asp Ala Leu Asp Leu

275 280 285

Pro Ser Phe Val Leu Asn Glu Ala Ile

290 295

<210> 87

<211> 282

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 ACU23599 BLAST 282aa

<400> 87

Met Ala Thr Ser Asn Phe Ser Ile Val Leu Ser Val Ser Leu Ala Phe

1 5 10 15

Phe Leu Val Leu Leu Thr Lys Ala His Pro Thr Asp Thr Val Ser Phe

20 25 30

Thr Phe Asn Lys Phe Asn Pro Val Gln Pro Asn Ile Met Leu Gln Lys

35 40 45

Asp Ala Ser Ile Ser Ser Ser Gly Val Leu Gln Leu Thr Lys Val Gly

50 55 60

Ser Asn Gly Val Pro Thr Ser Gly Ser Leu Gly Arg Ala Leu Tyr Ala

65 70 75 80

Ala Pro Ile Gln Ile Trp Asp Ser Glu Thr Gly Lys Val Ala Ser Trp

85 90 95

Ala Thr Ser Phe Lys Phe Asn Ile Phe Ala Pro Asn Lys Ser Asn Ser

100 105 110

Ala Asp Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Gln Pro Gln

115 120 125

Ser Asp Asp Gly Phe Leu Gly Leu Phe Asn Ser Pro Leu Lys Asp Lys

130 135 140

Ser Leu Gln Thr Val Ala Ile Glu Phe Asp Thr Phe Ser Asn Lys Lys

145 150 155 160

Trp Asp Pro Ala Asn Arg His Ile Gly Ile Asp Val Asp Ser Ile Lys

165 170 175

Ser Ile Lys Thr Ala Ser Trp Gly Leu Ser Asn Gly Gln Val Ala Glu

180 185 190

Ile Leu Val Thr Tyr Asn Ala Ala Thr Ser Leu Leu Val Ala Ser Leu

195 200 205

Ile His Pro Ser Lys Lys Thr Ser Tyr Ile Leu Ser Asp Thr Val Asn

210 215 220

Leu Lys Ser Asn Leu Pro Glu Trp Val Ser Val Gly Phe Ser Ala Thr

225 230 235 240

Thr Gly Leu His Glu Gly Ser Val Glu Thr His Asp Val Ile Ser Trp

245 250 255

Ser Phe Ala Ser Lys Leu Ser Asp Gly Ser Ser Asn Asp Ala Leu Asp

260 265 270

Leu Pro Ser Phe Val Leu Asn Glu Ala Ile

275 280

<210> 88

<211> 280

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 CAH60173 BLAST 280aa

<400> 88

Met Ala Ser Ser Lys Phe Ser Thr Val Ile Ser Phe Ser Leu Ala Leu

1 5 10 15

Phe Leu Val Leu Leu Thr Gln Ala Asn Ser Thr Asn Ile Phe Ser Phe

20 25 30

Asn Phe Gln Thr Phe Asp Ser Pro Asn Leu Ile Phe Gln Gly Asp Ala

35 40 45

Ser Val Ser Ser Ser Gly Gln Leu Arg Leu Thr Lys Val Lys Gly Asn

50 55 60

Gly Lys Pro Thr Ala Ala Ser Leu Gly Arg Ala Phe Tyr Ser Ala Pro

65 70 75 80

Ile Gln Ile Trp Asp Ser Thr Thr Gly Asn Val Ala Ser Phe Ala Thr

85 90 95

Ser Phe Thr Phe Asn Ile Leu Ala Pro Asn Lys Ser Asn Ser Ala Asp

100 105 110

Gly Leu Ala Phe Ala Leu Val Pro Val Gly Ser Gln Pro Lys Ser Asn

115 120 125

Gly Gly Phe Leu Gly Leu Phe Asp Asn Ala Thr Tyr Asp Ser Ser Ala

130 135 140

Gln Thr Val Ala Val Glu Phe Asp Thr Tyr Ser Asn Pro Lys Trp Asp

145 150 155 160

Pro Glu Asn Arg His Ile Gly Ile Asp Val Asn Ser Ile Glu Ser Ile

165 170 175

Arg Thr Ala Ser Trp Gly Leu Ala Asn Gly Gln Asn Ala Glu Ile Leu

180 185 190

Ile Thr Tyr Asp Ser Ser Thr Lys Leu Leu Val Ala Ser Leu Val His

195 200 205

Pro Ser Arg Arg Thr Ser Tyr Ile Val Ser Glu Arg Val Asp Leu Lys

210 215 220

Ser Val Leu Pro Glu Trp Val Ser Ile Gly Phe Ser Ala Thr Thr Gly

225 230 235 240

Leu Leu Glu Gly Ser Ile Glu Thr His Asp Val Leu Ser Trp Ser Phe

245 250 255

Ala Ser Lys Leu Ser Asp Asp Thr Thr Ser Glu Gly Leu Asn Leu Ala

260 265 270

Asn Phe Val Leu Asn Lys Ile Leu

275 280

<210> 89

<211> 228

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 Glyma10g01620.2 BLAST 228aa

<400> 89

Met Ala Thr Ser Lys Phe His Thr Gln Lys Pro Leu Phe Val Val Leu

1 5 10 15

Ser Val Val Val Val Leu Leu Thr Met Thr Lys Val Asn Ser Thr Lys

20 25 30

Pro Phe Leu Ser Pro Gly Thr Ser Ser Cys Arg Thr Asn Arg Thr Leu

35 40 45

Ile Leu Gln Gly Asp Ala Leu Val Thr Ser Ser Arg Lys Ser Leu Gly

50 55 60

Arg Ala Leu Tyr Ser Thr Pro Ile His Ile Trp Asp Ser Glu Ile Gly

65 70 75 80

Ser Val Ala Ser Phe Ala Ala Ser Phe Asn Phe Thr Val Tyr Ala Ser

85 90 95

Asp Ile Ala Asn Leu Ala Asp Gly Leu Ala Phe Phe Leu Ala Pro Ile

100 105 110

Asp Thr Gln Pro Gln Thr Arg Gly Gly Tyr Leu Gly Leu Tyr Asn Asn

115 120 125

Pro Ser Asn Ser Ser Trp Gly Leu Ala Asn Asp Gln Val Thr Asn Val

130 135 140

Leu Ile Thr Tyr Asp Ala Ser Thr Asn Leu Leu Val Ala Ser Leu Val

145 150 155 160

His Pro Ser Gln Arg Ser Ser Tyr Ile Leu Ser Asp Val Leu Asp Leu

165 170 175

Lys Val Ala Leu Pro Glu Trp Val Arg Ile Gly Phe Ser Ala Thr Thr

180 185 190

Gly Leu Asn Val Ala Ser Glu Thr His Asp Val His Ser Trp Ser Phe

195 200 205

Ser Ser Asn Leu Pro Phe Gly Ser Ser Asn Thr Asn Pro Ser Asp Phe

210 215 220

Ala Ile Phe Ile

225

<210> 90

<211> 285

<212> PRT

<213> 人工序列

<220>

<223> 凝集素 Glyma02g01590.1 BLAST 285aa

<400> 90

Met Ala Thr Ser Lys Leu Lys Thr Gln Asn Val Val Val Ser Leu Ser

1 5 10 15

Leu Thr Leu Thr Leu Val Leu Val Leu Leu Thr Ser Lys Ala Asn Ser

20 25 30

Ala Glu Thr Val Ser Phe Ser Trp Asn Lys Phe Val Pro Lys Gln Pro

35 40 45

Asn Met Ile Leu Gln Gly Asp Ala Ile Val Thr Ser Ser Gly Lys Leu

50 55 60

Gln Leu Asn Lys Val Asp Glu Asn Gly Thr Pro Lys Pro Ser Ser Leu

65 70 75 80

Gly Arg Ala Leu Tyr Ser Thr Pro Ile His Ile Trp Asp Lys Glu Thr

85 90 95

Gly Ser Val Ala Ser Phe Ala Ala Ser Phe Asn Phe Thr Phe Tyr Ala

100 105 110

Pro Asp Thr Lys Arg Leu Ala Asp Gly Leu Ala Phe Phe Leu Ala Pro

115 120 125

Ile Asp Thr Lys Pro Gln Thr His Ala Gly Tyr Leu Gly Leu Phe Asn

130 135 140

Glu Asn Glu Ser Gly Asp Gln Val Val Ala Val Glu Phe Asp Thr Phe

145 150 155 160

Arg Asn Ser Trp Asp Pro Pro Asn Pro His Ile Gly Ile Asn Val Asn

165 170 175

Ser Ile Arg Ser Ile Lys Thr Thr Ser Trp Asp Leu Ala Asn Asn Lys

180 185 190

Val Ala Lys Val Leu Ile Thr Tyr Asp Ala Ser Thr Ser Leu Leu Val

195 200 205

Ala Ser Leu Val Tyr Pro Ser Gln Arg Thr Ser Asn Ile Leu Ser Asp

210 215 220

Val Val Asp Leu Lys Thr Ser Leu Pro Glu Trp Val Arg Ile Gly Phe

225 230 235 240

Ser Ala Ala Thr Gly Leu Asp Ile Pro Gly Glu Ser His Asp Val Leu

245 250 255

Ser Trp Ser Phe Ala Ser Asn Leu Pro His Ala Ser Ser Asn Ile Asp

260 265 270

Pro Leu Asp Leu Thr Ser Phe Val Leu His Glu Ala Ile

275 280 285

<210> 91

<211> 6

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 91

Val Phe Ser Pro Asn Lys

1 5

<210> 92

<211> 13

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 92

Ala Asn Ser Thr Asn Thr Val Ser Phe Thr Val Ser Lys

1 5 10

<210> 93

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 93

Gln Gln Asn Leu Ile Phe Gln Gly Asp Ala Ala Ile Ser Pro Ser Gly

1 5 10 15

Val Leu Arg

<210> 94

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 94

Thr Ala Asp Gly Leu Ala Phe Phe Leu Ala Pro Val Gly Ser Lys Pro

1 5 10 15

Gln Ser Lys

<210> 95

<211> 864

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶共有序列

<400> 95

Met Gly Gly Ile Phe Asp Lys Gly Gln Lys Ile Lys Gly Thr Val Val

1 5 10 15

Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser Ile Gly

20 25 30

Lys Gly Gly Val Ile Asp Thr Ala Thr Gly Ile Leu Gly Ala Gly Val

35 40 45

Ser Leu Val Gly Gly Val Ile Asp Thr Ala Thr Ala Phe Leu Gly Arg

50 55 60

Asn Ile Ser Met Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly Ser Gly

65 70 75 80

Asn Gly Lys Val Gly Lys Glu Val Tyr Leu Glu Lys His Leu Pro Thr

85 90 95

Leu Pro Thr Leu Gly Ala Arg Gln Asp Ala Phe Ser Ile Phe Phe Glu

100 105 110

Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys Asn Phe

115 120 125

Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp Ile Pro

130 135 140

Asn His Gly Thr Ile Glu Phe Val Cys Asn Ser Trp Val Tyr Asn Phe

145 150 155 160

Lys Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr Tyr Leu

165 170 175

Pro Ser Ala Thr Pro Ala Pro Leu Leu Lys Tyr Arg Lys Glu Glu Leu

180 185 190

Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Lys Asp Phe Asp Arg

195 200 205

Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Gly Gly

210 215 220

Asp Pro Arg Pro Ile Leu Gly Gly Ser Ser Ile Tyr Pro Tyr Pro Arg

225 230 235 240

Arg Val Arg Thr Gly Arg Glu Arg Thr Arg Thr Asp Pro Asn Ser Glu

245 250 255

Lys Pro Gly Glu Val Tyr Val Pro Arg Asp Glu Asn Phe Gly His Leu

260 265 270

Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser His Asp

275 280 285

Val Ile Pro Leu Phe Lys Ser Ala Ile Phe Gln Leu Arg Val Thr Ser

290 295 300

Ser Glu Phe Asp Ser Phe Glu Asp Val Arg Ser Leu Tyr Glu Gly Gly

305 310 315 320

Ile Lys Leu Pro Thr Asp Ile Leu Ser Gln Ile Ser Pro Leu Pro Ala

325 330 335

Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Val Leu Gln Phe Pro

340 345 350

Pro Pro His Val Ala Lys Val Ser Lys Ser Gly Trp Met Thr Asp Glu

355 360 365

Glu Phe Ala Arg Glu Met Ile Ala Gly Val Asn Pro Asn Val Ile Arg

370 375 380

Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Thr Leu Tyr

385 390 395 400

Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu Gln Leu Glu Ile Asn Met

405 410 415

Gly Gly Val Thr Val Glu Glu Ala Leu Ser Thr Gln Arg Leu Phe Ile

420 425 430

Leu Asp Tyr Gln Asp Ala Phe Ile Pro Tyr Leu Thr Arg Ile Asn Ser

435 440 445

Leu Pro Thr Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe Leu Lys

450 455 460

Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys Pro His

465 470 475 480

Pro Asp Gly Asp Asn Leu Gly Pro Glu Ser Ile Val Val Leu Pro Ala

485 490 495

Thr Glu Gly Val Asp Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val

500 505 510

Ile Val Asn Asp Ser Gly Tyr His Gln Leu Val Ser His Trp Leu Asn

515 520 525

Thr His Ala Val Met Glu Pro Phe Ala Ile Ala Thr Asn Arg His Leu

530 535 540

Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His Tyr Arg Asp

545 550 555 560

Thr Ile Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala Asp

565 570 575

Gly Ile Ile Glu Lys Ser Phe Leu Pro Gly Lys Tyr Ser Ile Glu Met

580 585 590

Ser Ser Ser Val Tyr Lys Asn Trp Val Phe Thr Asp Gln Ala Leu Pro

595 600 605

Ala Asp Leu Val Lys Arg Gly Leu Ala Ile Glu Asp Pro Ser Ala Pro

610 615 620

His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly

625 630 635 640

Leu Glu Ile Trp Asp Ala Ile Lys Thr Trp Val His Glu Tyr Val Ser

645 650 655

Leu Tyr Tyr Pro Thr Asp Ala Ala Val Gln Gln Asp Thr Glu Leu Gln

660 665 670

Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu Lys Asp

675 680 685

Lys Pro Trp Trp Pro Lys Met Gln Thr Thr Glu Asp Leu Ile Gln Ser

690 695 700

Cys Ser Ile Ile Ile Trp Thr Ala Ser Ala Leu His Ala Ala Val Asn

705 710 715 720

Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn Arg Pro Thr Leu

725 730 735

Ala Arg Arg Phe Ile Pro Glu Glu Gly Thr Pro Glu Tyr Asp Glu Met

740 745 750

Val Lys Asn Pro Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe

755 760 765

Glu Thr Leu Ile Asp Leu Ser Val Ile Glu Ile Leu Ser Arg His Ala

770 775 780

Ser Asp Glu Ile Tyr Leu Gly Glu Arg Asp Thr Pro Asn Trp Thr Thr

785 790 795 800

Asp Lys Lys Ala Leu Glu Ala Phe Lys Lys Phe Gly Ser Lys Leu Thr

805 810 815

Gly Ile Glu Gly Lys Ile Asn Ala Arg Asn Ser Asp Pro Ser Leu Arg

820 825 830

Asn Arg Thr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu His Arg Ser

835 840 845

Ser Glu Glu Gly Leu Thr Phe Lys Gly Ile Pro Asn Ser Ile Ser Ile

850 855 860

<210> 96

<211> 864

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 2IUK_A BLAST 864aa

<400> 96

Met Phe Gly Ile Phe Asp Lys Gly Gln Lys Ile Lys Gly Thr Val Val

1 5 10 15

Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser Ile Gly

20 25 30

Lys Gly Gly Val Ile Asp Thr Ala Thr Gly Ile Leu Gly Gln Gly Val

35 40 45

Ser Leu Val Gly Gly Val Ile Asp Thr Ala Thr Ser Phe Leu Gly Arg

50 55 60

Asn Ile Ser Met Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly Ser Gly

65 70 75 80

Asn Gly Lys Val Gly Lys Glu Val Tyr Leu Glu Lys His Leu Pro Thr

85 90 95

Leu Pro Thr Leu Gly Ala Arg Gln Asp Ala Phe Ser Ile Phe Phe Glu

100 105 110

Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys Asn Phe

115 120 125

Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp Ile Pro

130 135 140

Asn His Gly Thr Ile Glu Phe Val Cys Asn Ser Trp Val Tyr Asn Phe

145 150 155 160

Arg Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr Tyr Leu

165 170 175

Pro Ser Ala Thr Pro Ala Pro Leu Leu Lys Tyr Arg Lys Glu Glu Phe

180 185 190

Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Lys Asp Phe Asp Arg

195 200 205

Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Gly Gly

210 215 220

Asp Pro Arg Pro Ile Leu Gly Gly Cys Ser Ile Tyr Pro Tyr Pro Leu

225 230 235 240

Arg Val Arg Thr Gly Arg Glu Arg Thr Arg Thr Asp Pro Asn Ser Glu

245 250 255

Lys Pro Gly Glu Val Tyr Val Pro Arg Asp Glu Asn Phe Gly His Leu

260 265 270

Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser His Asp

275 280 285

Val Ile Pro Leu Phe Lys Ser Ala Ile Phe Gln Leu Arg Val Thr Ser

290 295 300

Ser Glu Phe Glu Ser Phe Glu Asp Val Arg Ser Leu Tyr Glu Gly Gly

305 310 315 320

Ile Lys Leu Pro Thr Asp Ile Leu Ser Gln Ile Ser Pro Leu Pro Ala

325 330 335

Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Val Leu Gln Phe Pro

340 345 350

Pro Pro His Val Ala Lys Val Ser Lys Ser Gly Val Met Thr Asp Glu

355 360 365

Glu Phe Ala Arg Glu Val Ile Ala Gly Val Asn Pro Asn Val Ile Arg

370 375 380

Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Thr Leu Tyr

385 390 395 400

Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu Gln Leu Glu Ile Asn Met

405 410 415

Gly Gly Val Thr Val Glu Glu Ala Leu Ser Thr Gln Arg Leu Phe Ile

420 425 430

Leu Asp Tyr Gln Asp Ala Phe Ile Pro Tyr Leu Thr Arg Ile Asn Ser

435 440 445

Leu Pro Thr Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe Leu Lys

450 455 460

Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys Pro His

465 470 475 480

Pro Asp Gly Asp Asn Leu Gly Pro Glu Ser Ile Val Val Leu Pro Ala

485 490 495

Thr Glu Gly Val Asp Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val

500 505 510

Ile Val Asn Asp Ser Gly Tyr His Gln Leu Val Ser His Trp Leu Asn

515 520 525

Thr His Ala Val Met Glu Pro Phe Ala Ile Ala Thr Asn Arg His Leu

530 535 540

Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His Tyr Arg Asp

545 550 555 560

Thr Ile Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala Asp

565 570 575

Gly Ile Ile Glu Lys Ser Phe Leu Pro Gly Lys Tyr Ser Ile Glu Met

580 585 590

Ser Ser Ser Val Tyr Lys Asn Trp Val Phe Thr His Gln Ala Leu Pro

595 600 605

Ala Asp Leu Val Lys Arg Gly Leu Ala Ile Glu Asp Pro Ser Ala Pro

610 615 620

His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly

625 630 635 640

Leu Glu Ile Trp Asp Ala Ile Lys Thr Trp Val His Glu Tyr Val Ser

645 650 655

Leu Tyr Tyr Pro Thr Asp Ala Ala Val Gln Gln Asp Thr Glu Leu Gln

660 665 670

Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu Lys Glu

675 680 685

Lys Pro Trp Trp Pro Lys Lys Gln Thr Thr Glu Asp Leu Ile Gln Ser

690 695 700

Cys Ser Ile Ile Val Trp Thr Ala Ser Ala Leu His Ala Ala Val Asn

705 710 715 720

Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn Arg Pro Thr Leu

725 730 735

Ala Arg Arg Phe Ile Pro Ala Glu Gly Thr Pro Glu Tyr Asp Glu Met

740 745 750

Val Lys Asn Pro Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe

755 760 765

Glu Thr Leu Ile Asp Leu Ser Val Ile Glu Ile Leu Ser Arg His Ala

770 775 780

Ser Asp Glu Ile Tyr Leu Gly Glu Arg Glu Thr Pro Asn Trp Thr Thr

785 790 795 800

Asp Lys Lys Ala Leu Glu Ala Phe Lys Arg Phe Gly Ser Lys Leu Thr

805 810 815

Gly Ile Glu Gly Lys Ile Asn Ala Arg Asn Ser Asp Pro Ser Leu Arg

820 825 830

Asn Arg Thr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu His Arg Ser

835 840 845

Ser Glu Glu Gly Leu Thr Phe Lys Gly Ile Pro Asn Ser Ile Ser Ile

850 855 860

<210> 97

<211> 864

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 NP_001238676 BLAST 864aa

<400> 97

Met Phe Gly Ile Phe Asp Lys Gly Gln Lys Ile Lys Gly Thr Val Val

1 5 10 15

Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser Ile Gly

20 25 30

Lys Gly Gly Val Ile Asp Thr Ala Thr Gly Ile Leu Gly Gln Gly Val

35 40 45

Ser Leu Val Gly Gly Val Ile Asp Thr Ala Thr Ser Phe Leu Gly Arg

50 55 60

Asn Ile Ser Met Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly Ser Gly

65 70 75 80

Asn Gly Lys Val Gly Lys Glu Val Tyr Leu Glu Lys His Leu Pro Thr

85 90 95

Leu Pro Thr Leu Gly Ala Arg Gln Asp Ala Phe Ser Ile Phe Phe Glu

100 105 110

Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys Asn Phe

115 120 125

Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp Ile Pro

130 135 140

Asn His Gly Thr Ile Glu Phe Val Cys Asn Ser Trp Val Tyr Asn Phe

145 150 155 160

Arg Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr Tyr Leu

165 170 175

Pro Ser Ala Thr Pro Ala Pro Leu Leu Lys Tyr Arg Lys Glu Glu Leu

180 185 190

Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Lys Asp Phe Asp Arg

195 200 205

Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Gly Gly

210 215 220

Asp Pro Arg Pro Ile Leu Gly Gly Cys Ser Ile Tyr Pro Tyr Pro Leu

225 230 235 240

Arg Val Arg Thr Gly Arg Glu Arg Thr Arg Thr Asp Pro Asn Ser Glu

245 250 255

Lys Pro Gly Glu Val Tyr Val Pro Arg Asp Glu Asn Phe Gly His Leu

260 265 270

Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser His Asp

275 280 285

Val Ile Pro Leu Phe Lys Ser Ala Ile Phe Gln Leu Arg Val Thr Ser

290 295 300

Ser Glu Phe Glu Ser Phe Glu Asp Val Arg Ser Leu Tyr Glu Gly Gly

305 310 315 320

Ile Lys Leu Pro Thr Asp Ile Leu Ser Gln Ile Ser Pro Leu Pro Ala

325 330 335

Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Val Leu Gln Phe Pro

340 345 350

Pro Pro His Val Ala Lys Val Ser Lys Ser Gly Trp Met Thr Asp Glu

355 360 365

Glu Phe Ala Arg Glu Val Ile Ala Gly Val Asn Pro Asn Val Ile Arg

370 375 380

Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Thr Leu Tyr

385 390 395 400

Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu Gln Leu Glu Ile Asn Met

405 410 415

Gly Gly Val Thr Val Glu Glu Ala Leu Ser Thr Gln Arg Leu Phe Ile

420 425 430

Leu Asp Tyr Gln Asp Ala Phe Ile Pro Tyr Leu Thr Arg Ile Asn Ser

435 440 445

Leu Pro Thr Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe Leu Lys

450 455 460

Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys Pro His

465 470 475 480

Pro Asp Gly Asp Asn Leu Gly Pro Glu Ser Ile Val Val Leu Pro Ala

485 490 495

Thr Glu Gly Val Asp Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val

500 505 510

Ile Val Asn Asp Ser Gly Tyr His Gln Leu Val Ser His Trp Leu Asn

515 520 525

Thr His Ala Val Met Glu Pro Phe Ala Ile Ala Thr Asn Arg His Leu

530 535 540

Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His Tyr Arg Asp

545 550 555 560

Thr Ile Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala Asp

565 570 575

Gly Ile Ile Glu Lys Ser Phe Leu Pro Gly Lys Tyr Ser Ile Glu Met

580 585 590

Ser Ser Ser Val Tyr Lys Asn Trp Val Phe Thr His Gln Ala Leu Pro

595 600 605

Ala Asp Leu Val Lys Arg Gly Leu Ala Ile Glu Asp Pro Ser Ala Pro

610 615 620

His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly

625 630 635 640

Leu Glu Ile Trp Asp Ala Ile Lys Thr Trp Val His Glu Tyr Val Ser

645 650 655

Leu Tyr Tyr Pro Thr Asp Ala Ala Val Gln Gln Asp Thr Glu Leu Gln

660 665 670

Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu Lys Glu

675 680 685

Lys Pro Trp Trp Pro Lys Lys Gln Thr Thr Glu Asp Leu Ile Gln Ser

690 695 700

Cys Ser Ile Ile Val Trp Thr Ala Ser Ala Leu His Ala Ala Val Asn

705 710 715 720

Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn Arg Pro Thr Leu

725 730 735

Ala Arg Arg Phe Ile Pro Ala Glu Gly Thr Pro Glu Tyr Asp Glu Met

740 745 750

Val Lys Asn Pro Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe

755 760 765

Glu Thr Leu Ile Asp Leu Ser Val Ile Glu Ile Leu Ser Arg His Ala

770 775 780

Ser Asp Glu Ile Tyr Leu Gly Glu Arg Glu Thr Pro Asn Trp Thr Thr

785 790 795 800

Asp Lys Lys Ala Leu Glu Ala Phe Lys Arg Phe Gly Ser Lys Leu Thr

805 810 815

Gly Ile Glu Gly Lys Ile Asn Ala Arg Asn Ser Asp Pro Ser Leu Arg

820 825 830

Asn Arg Thr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu His Arg Ser

835 840 845

Ser Glu Glu Gly Leu Thr Phe Lys Gly Ile Pro Asn Ser Ile Ser Ile

850 855 860

<210> 98

<211> 867

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 Glyma08g20220.1 BLAST 867aa

<400> 98

Met Leu Gly Leu Phe Asp Lys Ser His Lys Ile Lys Gly Thr Val Val

1 5 10 15

Leu Met Pro Lys Ser Val Leu Asp Ile Asn Asp Leu Asn Ser Val Lys

20 25 30

Asn Gly Gly Val Gly Gly Val Val Ser Gly Ile Phe Gly Ala Val Ala

35 40 45

Asp Val Thr Gly Gln Ile Val Asp Thr Ala Thr Ala Ile Phe Ser Arg

50 55 60

Asn Val Ser Phe Lys Leu Ile Ser Ala Thr Ser Thr Asp Ala Lys Gly

65 70 75 80

Asn Gly Lys Val Gly Asn Glu Thr Phe Leu Glu Lys His Leu Pro Thr

85 90 95

Leu Pro Thr Leu Gly Asp Arg Arg Asp Ala Tyr Asp Ile His Phe Glu

100 105 110

Trp Asp Ala Asn Phe Gly Ile Pro Gly Ala Phe Tyr Ile Arg Asn Tyr

115 120 125

Thr Tyr Asp Glu Phe Phe Leu Val Ser Val Thr Leu Glu Asp Ile Pro

130 135 140

Asn His Gly Thr Ile His Phe Val Cys Asn Ser Trp Val Tyr Asn Phe

145 150 155 160

Lys Asp Tyr Asp Lys Lys Asp Arg Ile Phe Phe Ala Asn Lys Thr Tyr

165 170 175

Leu Pro Ser Ala Thr Pro Gly Pro Leu Val Lys Tyr Arg Glu Glu Glu

180 185 190

Leu Lys Ile Leu Arg Gly Asp Gly Thr Gly Glu Arg Lys Glu His Glu

195 200 205

Arg Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Glu

210 215 220

Asp Val Lys Leu Ala Arg Pro Val Leu Gly Gly Ser Ser Thr Tyr Pro

225 230 235 240

Tyr Pro Arg Arg Val Arg Thr Gly Arg Lys Ala Thr Lys Lys Asp Pro

245 250 255

Lys Ser Glu Arg Pro Ala Ser Glu Leu Tyr Met Pro Arg Asp Glu Lys

260 265 270

Phe Gly His Leu Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser

275 280 285

Leu Ser Gln Lys Leu Leu Pro Ser Leu Glu Asn Val Phe Asp Ser Asp

290 295 300

Leu Thr Trp Asn Glu Phe Asp Ser Phe Glu Glu Val Arg Asp Leu Tyr

305 310 315 320

Glu Gly Gly Ile Lys Val Pro Thr Gly Val Leu Ser Asp Ile Ser Pro

325 330 335

Ile Pro Ile Phe Lys Glu Ile Phe Arg Thr Asp Gly Glu Ser Val Leu

340 345 350

Gln Phe Pro Pro Pro His Val Val Gln Val Thr Lys Ser Ala Trp Met

355 360 365

Thr Asp Asp Glu Phe Ala Arg Glu Met Ile Ala Gly Val Asn Pro Asn

370 375 380

Val Ile Arg Leu Leu Lys Glu Phe Pro Pro Gln Ser Lys Leu Asp Pro

385 390 395 400

Ser Leu Tyr Gly Asp Gln Ser Ser Thr Ile Thr Lys Glu His Leu Glu

405 410 415

Ile Asn Met Asp Gly Val Thr Val Glu Glu Ala Leu Asn Gly Gln Arg

420 425 430

Leu Phe Ile Leu Asp Tyr Gln Asp Ala Phe Met Pro Tyr Leu Thr Arg

435 440 445

Ile Asn Ala Leu Pro Ser Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu

450 455 460

Leu Leu Lys Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser

465 470 475 480

Lys Pro His Pro Ser Gly Asp Asn Leu Gly Ala Glu Ser Lys Val Val

485 490 495

Leu Pro Ala Asp Gln Gly Val Glu Ser Thr Ile Trp Leu Leu Ala Lys

500 505 510

Ala His Val Ile Val Asn Asp Ser Gly Tyr His Gln Leu Met Ser His

515 520 525

Trp Leu Asn Thr His Ala Val Thr Glu Pro Phe Ile Ile Ala Thr Asn

530 535 540

Arg Arg Leu Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His

545 550 555 560

Tyr Arg Asp Thr Ile Asn Ile Asn Gly Leu Ala Arg Asn Ala Leu Ile

565 570 575

Asn Ala Gly Gly Val Ile Glu Glu Ser Phe Leu Pro Gly Arg Tyr Ser

580 585 590

Ile Glu Met Ser Ser Ala Val Tyr Lys Asn Trp Val Phe Thr Asp Gln

595 600 605

Ala Leu Pro Val Asp Leu Ile Lys Arg Gly Met Ala Val Glu Asp Pro

610 615 620

Ser Ser Pro His Gly Leu Arg Leu Ala Val Glu Asp Tyr Pro Tyr Ala

625 630 635 640

Val Asp Gly Leu Glu Ile Trp Asp Ala Ile Lys Ser Trp Val Gln Glu

645 650 655

Tyr Val Ser Leu Tyr Tyr Pro Thr Asp Leu Ala Ile Gln Gln Asp Thr

660 665 670

Glu Leu Gln Ala Trp Trp Lys Glu Val Val Glu Lys Gly His Gly Asp

675 680 685

Leu Lys Asp Lys Pro Trp Trp Pro Lys Met Gln Thr Arg Gln Glu Leu

690 695 700

Ile Gln Ser Cys Ser Thr Ile Ile Trp Ile Ala Ser Ala Leu His Ala

705 710 715 720

Ala Val Asn Phe Gly Gln Tyr Pro Tyr Gly Gly Phe Ile Leu Asn Arg

725 730 735

Pro Thr Leu Ser Arg Arg Trp Ile Pro Glu Pro Gly Thr Lys Glu Tyr

740 745 750

Asp Glu Met Val Glu Ser Pro Gln Thr Ala Tyr Leu Arg Thr Ile Thr

755 760 765

Pro Lys Arg Gln Thr Ile Ile Asp Leu Thr Val Ile Glu Ile Leu Ser

770 775 780

Arg His Ala Ser Asp Glu Ile Tyr Leu Gly Glu Arg Asp Asn Pro Asn

785 790 795 800

Trp Thr Ser Asp Ser Lys Ala Leu Glu Ala Phe Lys Lys Phe Gly Ser

805 810 815

Lys Leu Ala Glu Ile Glu Gly Lys Ile Thr Ala Arg Asn Lys Asp Ser

820 825 830

Asn Lys Lys Asn Arg Tyr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu

835 840 845

Leu Pro Thr Ser Glu Glu Gly Leu Thr Phe Arg Gly Ile Pro Asn Ser

850 855 860

Ile Ser Ile

865

<210> 99

<211> 864

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 P24095 BLAST 864aa

<400> 99

Met Phe Gly Ile Phe Asp Lys Gly Gln Lys Ile Lys Gly Thr Val Val

1 5 10 15

Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser Ile Gly

20 25 30

Lys Gly Gly Val Ile Asp Thr Ala Thr Gly Ile Leu Gly Gln Gly Val

35 40 45

Ser Leu Val Gly Gly Val Ile Asp Thr Ala Thr Ser Phe Leu Gly Arg

50 55 60

Asn Ile Ser Met Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly Ser Gly

65 70 75 80

Asn Gly Lys Val Gly Lys Glu Val Tyr Leu Glu Lys His Leu Pro Thr

85 90 95

Leu Pro Thr Leu Gly Ala Arg Gln Asp Ala Phe Ser Ile Phe Phe Glu

100 105 110

Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys Asn Phe

115 120 125

Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp Ile Pro

130 135 140

Asn His Gly Thr Ile Glu Phe Val Cys Asn Ser Trp Val Tyr Asn Phe

145 150 155 160

Arg Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr Tyr Leu

165 170 175

Pro Ser Ala Thr Pro Ala Pro Leu Leu Lys Tyr Arg Lys Glu Glu Leu

180 185 190

Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Lys Asp Phe Asp Arg

195 200 205

Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Gly Gly

210 215 220

Asp Pro Arg Pro Ile Leu Gly Gly Ser Ser Ile Tyr Pro Tyr Pro Arg

225 230 235 240

Arg Val Arg Thr Gly Arg Glu Arg Thr Arg Thr Asp Pro Asn Ser Glu

245 250 255

Lys Pro Gly Glu Val Tyr Val Pro Arg Asp Glu Asn Phe Gly His Leu

260 265 270

Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser His Asp

275 280 285

Val Ile Pro Leu Phe Lys Ser Ala Ile Phe Gln Leu Arg Val Thr Ser

290 295 300

Ser Glu Phe Glu Ser Phe Glu Asp Val Arg Ser Leu Tyr Glu Gly Gly

305 310 315 320

Ile Lys Leu Pro Thr Asp Ile Leu Ser Gln Ile Ser Pro Leu Pro Ala

325 330 335

Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Val Leu Gln Phe Pro

340 345 350

Pro Pro His Val Ala Lys Val Ser Lys Ser Gly Trp Met Thr Asp Glu

355 360 365

Glu Phe Ala Arg Glu Val Ile Ala Gly Val Asn Pro Asn Val Ile Arg

370 375 380

Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Thr Leu Tyr

385 390 395 400

Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu Gln Leu Glu Ile Asn Met

405 410 415

Gly Gly Val Thr Val Glu Glu Ala Leu Ser Thr Gln Arg Leu Phe Ile

420 425 430

Leu Asp Tyr Gln Asp Ala Phe Ile Pro Tyr Leu Thr Arg Ile Asn Ser

435 440 445

Leu Pro Thr Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe Leu Lys

450 455 460

Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys Pro His

465 470 475 480

Pro Asp Gly Asp Asn Leu Gly Pro Glu Ser Ile Val Val Leu Pro Ala

485 490 495

Thr Glu Gly Val Asp Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val

500 505 510

Ile Val Asn Asp Ser Gly Tyr His Gln Leu Val Ser His Trp Leu Asn

515 520 525

Thr His Ala Val Met Glu Pro Phe Ala Ile Ala Thr Asn Arg His Leu

530 535 540

Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His Tyr Arg Asp

545 550 555 560

Thr Ile Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala Asp

565 570 575

Gly Ile Ile Glu Lys Ser Phe Leu Pro Gly Lys Tyr Ser Ile Glu Met

580 585 590

Ser Ser Ser Val Tyr Lys Asn Trp Val Phe Thr Asp Gln Ala Leu Pro

595 600 605

Ala Asp Leu Val Lys Arg Gly Leu Ala Ile Glu Asp Pro Ser Ala Pro

610 615 620

His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly

625 630 635 640

Leu Glu Ile Trp Asp Ala Ile Lys Thr Trp Val His Glu Tyr Val Ser

645 650 655

Leu Tyr Tyr Pro Thr Asp Ala Ala Val Gln Gln Asp Thr Glu Leu Gln

660 665 670

Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu Lys Glu

675 680 685

Lys Pro Trp Trp Pro Lys Met Gln Thr Thr Glu Asp Leu Ile Gln Ser

690 695 700

Cys Ser Ile Ile Val Trp Thr Ala Ser Ala Leu His Ala Ala Val Asn

705 710 715 720

Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn Arg Pro Thr Leu

725 730 735

Ala Arg Arg Phe Ile Pro Ala Glu Gly Thr Pro Glu Tyr Asp Glu Met

740 745 750

Val Lys Asn Pro Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe

755 760 765

Glu Thr Leu Ile Asp Leu Ser Val Ile Glu Ile Leu Ser Arg His Ala

770 775 780

Ser Asp Glu Ile Tyr Leu Gly Glu Arg Glu Thr Pro Asn Trp Thr Thr

785 790 795 800

Asp Lys Lys Ala Leu Glu Ala Phe Lys Arg Phe Gly Ser Lys Leu Thr

805 810 815

Gly Ile Glu Gly Lys Ile Asn Ala Arg Asn Ser Asp Pro Ser Leu Arg

820 825 830

Asn Arg Thr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu His Arg Ser

835 840 845

Ser Glu Glu Gly Leu Thr Phe Lys Gly Ile Pro Asn Ser Ile Ser Ile

850 855 860

<210> 100

<211> 866

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 Glyma07g00900.1 BLAST 866aa

<400> 100

Met Thr Gly Gly Met Phe Gly Arg Lys Gly Gln Lys Ile Lys Gly Thr

1 5 10 15

Val Val Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser

20 25 30

Val Gly Lys Gly Ser Ala Lys Asp Thr Ala Thr Asp Phe Leu Gly Lys

35 40 45

Gly Leu Asp Ala Leu Gly His Ala Val Asp Ala Leu Thr Ala Phe Ala

50 55 60

Gly His Ser Ile Ser Leu Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly

65 70 75 80

Ser Gly Lys Gly Lys Val Gly Asn Glu Ala Tyr Leu Glu Lys His Leu

85 90 95

Pro Thr Leu Pro Thr Leu Gly Ala Arg Gln Glu Ala Phe Asp Ile Asn

100 105 110

Phe Glu Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys

115 120 125

Asn Phe Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp

130 135 140

Ile Pro Asn His Gly Thr Ile Asn Phe Val Cys Asn Ser Trp Val Tyr

145 150 155 160

Asn Phe Lys Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr

165 170 175

Tyr Leu Pro Ser Ala Thr Pro Ala Pro Leu Leu Lys Tyr Arg Lys Glu

180 185 190

Glu Leu Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Lys Asp Phe

195 200 205

Asp Arg Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp

210 215 220

Gly Gly Asp Pro Arg Pro Ile Leu Gly Gly Ser Ser Ile Tyr Pro Tyr

225 230 235 240

Pro Arg Arg Val Arg Thr Gly Arg Glu Arg Thr Arg Thr Asp Pro Asn

245 250 255

Ser Glu Lys Pro Gly Glu Val Tyr Val Pro Arg Asp Glu Asn Phe Gly

260 265 270

His Leu Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser

275 280 285

His Asp Val Ile Pro Leu Phe Lys Ser Ala Ile Phe Gln Leu Arg Val

290 295 300

Thr Ser Ser Glu Phe Glu Ser Phe Glu Asp Val Arg Ser Leu Tyr Glu

305 310 315 320

Gly Gly Ile Lys Leu Pro Thr Asp Ile Leu Ser Gln Ile Ser Pro Leu

325 330 335

Pro Ala Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Val Leu Gln

340 345 350

Phe Pro Pro Pro His Val Ala Lys Val Ser Lys Ser Gly Trp Met Thr

355 360 365

Asp Glu Glu Phe Ala Arg Glu Val Ile Ala Gly Val Asn Pro Asn Val

370 375 380

Ile Arg Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Thr

385 390 395 400

Leu Tyr Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu Gln Leu Glu Ile

405 410 415

Asn Met Gly Gly Val Thr Val Glu Glu Ala Leu Ser Thr Gln Arg Leu

420 425 430

Phe Ile Leu Asp Tyr Gln Asp Ala Phe Ile Pro Tyr Leu Thr Arg Ile

435 440 445

Asn Ser Leu Pro Thr Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe

450 455 460

Leu Lys Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys

465 470 475 480

Pro His Pro Asp Gly Asp Asn Leu Gly Pro Glu Ser Ile Val Val Leu

485 490 495

Pro Ala Thr Glu Gly Val Asp Ser Thr Ile Trp Leu Leu Ala Lys Ala

500 505 510

His Val Ile Val Asn Asp Ser Gly Tyr His Gln Leu Val Ser His Trp

515 520 525

Leu Asn Thr His Ala Val Met Glu Pro Phe Ala Ile Ala Thr Asn Arg

530 535 540

His Leu Ser Val Leu His Pro Ile Tyr Lys Leu Leu Tyr Pro His Tyr

545 550 555 560

Arg Asp Thr Ile Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn

565 570 575

Ala Asp Gly Ile Ile Glu Lys Ser Phe Leu Pro Gly Lys Tyr Ser Ile

580 585 590

Glu Met Ser Ser Ser Val Tyr Lys Asn Trp Val Phe Thr Asp Gln Ala

595 600 605

Leu Pro Ala Asp Leu Val Lys Arg Gly Leu Ala Ile Glu Asp Pro Ser

610 615 620

Ala Pro His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val

625 630 635 640

Asp Gly Leu Glu Ile Trp Asp Ala Ile Lys Thr Trp Val His Glu Tyr

645 650 655

Val Ser Leu Tyr Tyr Pro Thr Asp Ala Ala Val Gln Gln Asp Thr Glu

660 665 670

Leu Gln Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu

675 680 685

Lys Glu Lys Pro Trp Trp Pro Lys Met Gln Thr Thr Glu Asp Leu Ile

690 695 700

Gln Ser Cys Ser Ile Ile Val Trp Thr Ala Ser Ala Leu His Ala Ala

705 710 715 720

Val Asn Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn Arg Pro

725 730 735

Thr Leu Ala Arg Arg Phe Ile Pro Ala Glu Gly Thr Pro Glu Tyr Asp

740 745 750

Glu Met Val Lys Asn Pro Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro

755 760 765

Lys Phe Glu Thr Leu Ile Asp Leu Ser Val Ile Glu Ile Leu Ser Arg

770 775 780

His Ala Ser Asp Glu Ile Tyr Leu Gly Glu Arg Glu Thr Pro Asn Trp

785 790 795 800

Thr Thr Asp Lys Lys Ala Leu Glu Ala Phe Lys Arg Phe Gly Ser Lys

805 810 815

Leu Thr Gly Ile Glu Gly Lys Ile Asn Ala Arg Asn Ser Asp Pro Ser

820 825 830

Leu Arg Asn Arg Thr Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu His

835 840 845

Arg Ser Ser Glu Glu Gly Leu Thr Phe Lys Gly Ile Pro Asn Ser Ile

850 855 860

Ser Ile

865

<210> 101

<211> 859

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 NP_001235189 BLAST 859aa

<400> 101

Met Thr Gly Gly Met Phe Gly Arg Lys Gly Gln Lys Ile Lys Gly Thr

1 5 10 15

Val Val Leu Met Pro Lys Asn Val Leu Asp Phe Asn Ala Ile Thr Ser

20 25 30

Val Gly Lys Gly Ser Ala Lys Asp Thr Ala Thr Asp Phe Leu Gly Lys

35 40 45

Gly Leu Asp Ala Leu Gly His Ala Val Asp Ala Leu Thr Ala Phe Ala

50 55 60

Gly His Ser Ile Ser Leu Gln Leu Ile Ser Ala Thr Gln Thr Asp Gly

65 70 75 80

Ser Gly Lys Gly Lys Val Gly Asn Glu Ala Tyr Leu Glu Lys His Leu

85 90 95

Pro Thr Leu Pro Thr Leu Gly Ala Arg Gln Glu Ala Phe Asp Ile Asn

100 105 110

Phe Glu Trp Asp Ala Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys

115 120 125

Asn Phe Met Thr Asp Glu Phe Phe Leu Val Ser Val Lys Leu Glu Asp

130 135 140

Ile Pro Asn His Gly Thr Ile Asn Phe Val Cys Asn Ser Trp Val Tyr

145 150 155 160

Asn Phe Lys Ser Tyr Lys Lys Asn Arg Ile Phe Phe Val Asn Asp Thr

165 170 175

Tyr Leu Pro Ser Ala Thr Pro Gly Pro Leu Val Lys Tyr Arg Gln Glu

180 185 190

Glu Leu Glu Val Leu Arg Gly Asp Gly Thr Gly Lys Arg Arg Asp Phe

195 200 205

Asp Arg Ile Tyr Asp Tyr Asp Ile Tyr Asn Asp Leu Gly Asn Pro Asp

210 215 220

Gly Gly Asp Pro Arg Pro Ile Ile Gly Gly Ser Ser Asn Tyr Pro Tyr

225 230 235 240

Pro Arg Arg Val Arg Thr Gly Arg Glu Lys Thr Arg Lys Asp Pro Asn

245 250 255

Ser Glu Lys Pro Gly Glu Ile Tyr Val Pro Arg Asp Glu Asn Phe Gly

260 265 270

His Leu Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys Ser Leu Ser

275 280 285

Gln Asn Val Ile Pro Leu Phe Lys Ser Ile Ile Leu Asn Leu Arg Val

290 295 300

Thr Ser Ser Glu Phe Asp Ser Phe Asp Glu Val Arg Gly Leu Phe Glu

305 310 315 320

Gly Gly Ile Lys Leu Pro Thr Asn Ile Leu Ser Gln Ile Ser Pro Leu

325 330 335

Pro Val Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Asn Thr Leu Gln

340 345 350

Phe Pro Pro Pro His Val Ile Arg Val Ser Lys Ser Gly Trp Met Thr

355 360 365

Asp Asp Glu Phe Ala Arg Glu Met Ile Ala Gly Val Asn Pro Asn Val

370 375 380

Ile Arg Arg Leu Gln Glu Phe Pro Pro Lys Ser Thr Leu Asp Pro Ala

385 390 395 400

Thr Tyr Gly Asp Gln Thr Ser Thr Ile Thr Lys Gln Gln Leu Glu Ile

405 410 415

Asn Leu Gly Gly Val Thr Val Glu Glu Ala Ile Ser Ala His Arg Leu

420 425 430

Phe Ile Leu Asp Tyr His Asp Ala Phe Phe Pro Tyr Leu Thr Lys Ile

435 440 445

Asn Ser Leu Pro Ile Ala Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe

450 455 460

Leu Lys Asp Asp Gly Ser Leu Lys Pro Leu Ala Ile Glu Leu Ser Lys

465 470 475 480

Pro Ala Thr Val Ser Lys Val Val Leu Pro Ala Thr Glu Gly Val Glu

485 490 495

Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val Ile Val Asn Asp Ser

500 505 510

Gly Tyr His Gln Leu Ile Ser His Trp Leu Asn Thr His Ala Val Met

515 520 525

Glu Pro Phe Ala Ile Ala Thr Asn Arg His Leu Ser Val Leu His Pro

530 535 540

Ile Tyr Lys Leu Leu Tyr Pro His Tyr Lys Asp Thr Ile Asn Ile Asn

545 550 555 560

Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala Gly Gly Ile Ile Glu Gln

565 570 575

Thr Phe Leu Pro Gly Lys Tyr Ser Ile Glu Met Ser Ser Val Val Tyr

580 585 590

Lys Asn Trp Val Phe Thr Asp Gln Ala Leu Pro Ala Asp Leu Val Lys

595 600 605

Arg Gly Leu Ala Val Glu Asp Pro Ser Ala Pro His Gly Leu Arg Leu

610 615 620

Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly Leu Glu Ile Trp Asp

625 630 635 640

Ala Ile Lys Thr Trp Val His Glu Tyr Val Ser Val Tyr Tyr Pro Thr

645 650 655

Asn Ala Ala Ile Gln Gln Asp Thr Glu Leu Gln Ala Trp Trp Lys Glu

660 665 670

Val Val Glu Lys Gly His Gly Asp Leu Lys Asp Lys Pro Trp Trp Pro

675 680 685

Lys Leu Gln Thr Val Glu Asp Leu Ile Gln Ser Cys Ser Ile Ile Ile

690 695 700

Trp Thr Ala Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln Tyr Pro

705 710 715 720

Tyr Gly Gly Tyr Ile Val Asn Arg Pro Thr Leu Ala Arg Arg Phe Ile

725 730 735

Pro Glu Glu Gly Thr Lys Glu Tyr Asp Glu Met Val Lys Asp Pro Gln

740 745 750

Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe Glu Thr Leu Ile Asp

755 760 765

Ile Ser Val Ile Glu Ile Leu Ser Arg His Ala Ser Asp Glu Val Tyr

770 775 780

Leu Gly Gln Arg Asp Asn Pro Asn Trp Thr Thr Asp Ser Lys Ala Leu

785 790 795 800

Glu Ala Phe Lys Lys Phe Gly Asn Lys Leu Ala Glu Ile Glu Gly Lys

805 810 815

Ile Thr Gln Arg Asn Asn Asp Pro Ser Leu Lys Ser Arg His Gly Pro

820 825 830

Val Gln Leu Pro Tyr Thr Leu Leu His Arg Ser Ser Glu Glu Gly Met

835 840 845

Ser Phe Lys Gly Ile Pro Asn Ser Ile Ser Ile

850 855

<210> 102

<211> 865

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 Glyma07g03910.1 BLAST 865aa

<400> 102

Met Phe Gly Ile Leu Gly Gly Asn Lys Gly His Lys Ile Lys Gly Thr

1 5 10 15

Val Val Leu Met Ser Lys Asn Val Leu Asp Phe Asn Glu Ile Val Ser

20 25 30

Thr Thr Gln Gly Gly Leu Val Gly Ala Ala Thr Gly Ile Phe Gly Ala

35 40 45

Ala Thr Gly Ile Val Gly Gly Val Val Asp Gly Ala Thr Ala Ile Phe

50 55 60

Ser Arg Asn Ile Ala Ile Gln Leu Ile Ser Ala Thr Lys Thr Asp Gly

65 70 75 80

Leu Gly Asn Gly Lys Val Gly Lys Gln Thr Tyr Leu Glu Lys His Leu

85 90 95

Pro Ser Leu Pro Thr Leu Gly Asp Arg Gln Asp Ala Phe Ser Val Tyr

100 105 110

Phe Glu Trp Asp Asn Asp Phe Gly Ile Pro Gly Ala Phe Tyr Ile Lys

115 120 125

Asn Phe Met Gln Ser Glu Phe Phe Leu Val Ser Val Thr Leu Glu Asp

130 135 140

Ile Pro Asn His Gly Thr Ile His Phe Val Cys Asn Ser Trp Val Tyr

145 150 155 160

Asn Ala Lys Ser Tyr Lys Arg Asp Arg Ile Phe Phe Ala Asn Lys Thr

165 170 175

Tyr Leu Pro Asn Glu Thr Pro Thr Pro Leu Val Lys Tyr Arg Lys Glu

180 185 190

Glu Leu Glu Asn Leu Arg Gly Asp Gly Lys Gly Glu Arg Lys Glu Tyr

195 200 205

Asp Arg Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp

210 215 220

Lys Ser Asn Asp Leu Ala Arg Pro Val Leu Gly Gly Ser Ser Ala Tyr

225 230 235 240

Pro Tyr Pro Arg Arg Gly Arg Thr Gly Arg Lys Pro Thr Thr Lys Asp

245 250 255

Ser Lys Ser Glu Ser Pro Ser Ser Ser Thr Tyr Ile Pro Arg Asp Glu

260 265 270

Asn Phe Gly His Leu Lys Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys

275 280 285

Ser Ile Ala Gln Thr Val Leu Pro Thr Phe Gln Ser Ala Phe Gly Leu

290 295 300

Asn Ala Glu Phe Asp Arg Phe Asp Asp Val Arg Gly Leu Phe Glu Gly

305 310 315 320

Gly Ile His Leu Pro Thr Asp Ala Leu Ser Lys Ile Ser Pro Leu Pro

325 330 335

Val Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Gln Val Leu Lys Phe

340 345 350

Pro Pro Pro His Val Ile Lys Val Ser Lys Ser Ala Trp Met Thr Asp

355 360 365

Glu Glu Phe Gly Arg Glu Met Leu Ala Gly Val Asn Pro Cys Leu Ile

370 375 380

Glu Cys Leu Gln Val Phe Pro Pro Lys Ser Lys Leu Asp Pro Thr Val

385 390 395 400

Tyr Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu His Leu Glu Ile Asn

405 410 415

Leu Gly Gly Leu Ser Val Glu Gln Ala Leu Ser Gly Asn Arg Leu Phe

420 425 430

Ile Leu Asp His His Asp Ala Phe Ile Ala Tyr Leu Arg Lys Ile Asn

435 440 445

Asp Leu Pro Thr Ala Lys Ser Tyr Ala Thr Arg Thr Ile Leu Phe Leu

450 455 460

Lys Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Leu Pro

465 470 475 480

His Pro Arg Gly Asp Glu Phe Gly Ala Val Ser Arg Val Val Leu Pro

485 490 495

Ala Asp Gln Gly Ala Glu Ser Thr Ile Trp Leu Ile Ala Lys Ala Tyr

500 505 510

Val Val Val Asn Asp Ser Cys Tyr His Gln Leu Met Ser His Trp Leu

515 520 525

Asn Thr His Ala Val Ile Glu Pro Phe Val Ile Ala Thr Asn Arg His

530 535 540

Leu Ser Val Leu His Pro Ile Tyr Lys Leu Leu Leu Pro His Tyr Arg

545 550 555 560

Asp Thr Met Asn Ile Asn Gly Leu Ala Arg Gln Ser Leu Ile Asn Ala

565 570 575

Gly Gly Ile Ile Glu Gln Ser Phe Leu Pro Gly Pro Phe Ala Val Glu

580 585 590

Met Ser Ser Ala Val Tyr Lys Gly Trp Val Phe Thr Asp Gln Ala Leu

595 600 605

Pro Ala Asp Leu Ile Lys Arg Gly Met Ala Val Glu Asp Pro Ser Ser

610 615 620

Pro Tyr Gly Leu Arg Leu Val Ile Asp Asp Tyr Pro Tyr Ala Val Asp

625 630 635 640

Gly Leu Glu Ile Trp Ser Ala Ile Gln Thr Trp Val Lys Asp Tyr Val

645 650 655

Ser Leu Tyr Tyr Ala Thr Asp Asp Ala Val Lys Lys Asp Ser Glu Leu

660 665 670

Gln Ala Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu Lys

675 680 685

Asp Lys Pro Trp Trp Pro Lys Leu Asn Thr Leu Gln Asp Leu Ile His

690 695 700

Ile Cys Cys Ile Ile Ile Trp Thr Ala Ser Ala Leu His Ala Ala Val

705 710 715 720

Asn Phe Gly Gln Tyr Pro Tyr Gly Gly Phe Ile Leu Asn Arg Pro Thr

725 730 735

Leu Thr Arg Arg Leu Leu Pro Glu Pro Gly Thr Lys Glu Tyr Gly Glu

740 745 750

Leu Thr Ser Asn His Gln Lys Ala Tyr Leu Arg Thr Ile Thr Gly Lys

755 760 765

Thr Glu Ala Leu Val Asp Leu Thr Val Ile Glu Ile Leu Ser Arg His

770 775 780

Ala Ser Asp Glu Val Tyr Leu Gly Gln Arg Asp Asn Pro Asn Trp Thr

785 790 795 800

Asp Asp Thr Lys Ala Ile Gln Ala Phe Lys Lys Phe Gly Asn Lys Leu

805 810 815

Lys Glu Ile Glu Asp Lys Ile Ser Gly Arg Asn Lys Asn Ser Ser Leu

820 825 830

Arg Asn Arg Asn Gly Pro Ala Gln Met Pro Tyr Thr Val Leu Leu Pro

835 840 845

Thr Ser Gly Glu Gly Leu Thr Phe Arg Gly Ile Pro Asn Ser Ile Ser

850 855 860

Ile

865

<210> 103

<211> 868

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 Glyma07g03920.2 BLAST 868aa

<400> 103

Met Leu Ile Gly Ser Leu Leu Asn Arg Arg Pro Lys Ile Lys Gly Thr

1 5 10 15

Val Val Leu Met Thr Lys Asn Val Phe Asp Val Asn Asp Phe Met Ala

20 25 30

Thr Thr Arg Gly Gly Pro Ala Ala Val Ala Gly Gly Ile Phe Gly Ala

35 40 45

Ala Gln Asp Ile Val Gly Gly Ile Val Asp Gly Ala Thr Ala Ile Phe

50 55 60

Ser Arg Asn Ile Ala Ile Gln Leu Ile Ser Ala Thr Lys Ser Glu Asn

65 70 75 80

Ala Leu Gly His Gly Lys Val Gly Lys Leu Thr Tyr Leu Glu Lys His

85 90 95

Leu Pro Ser Leu Pro Asn Leu Gly Asp Arg Gln Asp Ala Phe Asp Val

100 105 110

Tyr Phe Glu Trp Asp Glu Ser Phe Gly Ile Pro Gly Ala Phe Tyr Ile

115 120 125

Lys Asn Tyr Met Gln Ser Glu Phe Phe Leu Val Ser Phe Lys Leu Glu

130 135 140

Asp Val Pro Asn His Gly Thr Ile Leu Phe Ala Cys Asn Ser Trp Val

145 150 155 160

Tyr Asn Ala Lys Leu Tyr Lys Lys Asp Arg Ile Phe Phe Ala Asn Lys

165 170 175

Ala Tyr Leu Pro Asn Asp Thr Pro Thr Pro Leu Val Lys Tyr Arg Lys

180 185 190

Glu Glu Leu Glu Asn Leu Arg Gly Asp Gly Arg Gly Glu Arg Lys Glu

195 200 205

Leu Asp Arg Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro

210 215 220

Asp Glu Asn Asp Asp Leu Ala Arg Pro Ile Leu Gly Gly Ser Ser Lys

225 230 235 240

His Pro Tyr Pro Arg Arg Gly Arg Thr Gly Arg Lys Pro Thr Lys Lys

245 250 255

Asp Pro Arg Cys Glu Arg Pro Thr Ser Asp Thr Tyr Ile Pro Arg Asp

260 265 270

Glu Asn Phe Gly His Leu Lys Ser Ser Asp Phe Leu Thr Tyr Ala Ile

275 280 285

Lys Ser Leu Thr Gln Asn Val Leu Pro Gln Phe Asn Thr Ala Phe Gly

290 295 300

Phe Asn Asn Glu Phe Asp Ser Phe Glu Asp Val Arg Cys Leu Phe Asp

305 310 315 320

Gly Gly Val Tyr Leu Pro Thr Asp Val Leu Ser Lys Ile Ser Pro Ile

325 330 335

Pro Val Leu Lys Glu Ile Phe Arg Thr Asp Gly Glu Gln Ala Leu Lys

340 345 350

Phe Pro Pro Pro His Val Ile Lys Val Arg Glu Ser Glu Trp Met Thr

355 360 365

Asp Glu Glu Phe Gly Arg Glu Met Leu Ala Gly Val Asn Pro Gly Met

370 375 380

Ile Gln Arg Leu Gln Glu Phe Pro Pro Lys Ser Lys Leu Asp Pro Thr

385 390 395 400

Glu Phe Gly Asp Gln Thr Ser Thr Ile Thr Lys Glu His Leu Glu Ile

405 410 415

Asn Leu Gly Gly Leu Thr Val Glu Gln Ala Leu Lys Gly Asn Lys Leu

420 425 430

Phe Ile Leu Asp His His Asp Ala Phe Ile Pro Phe Met Asn Leu Ile

435 440 445

Asn Gly Leu Pro Thr Ala Lys Ser Tyr Ala Thr Arg Thr Ile Leu Phe

450 455 460

Leu Gln Asp Asp Gly Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Leu

465 470 475 480

Pro His Pro Arg Gly His Glu Phe Gly Ala Asp Ser Arg Val Val Leu

485 490 495

Pro Pro Ala Ala Val Asn Ser Ala Glu Gly Thr Ile Trp Leu Ile Ala

500 505 510

Lys Ala Tyr Val Ala Val Asn Asp Thr Gly Tyr His Gln Leu Ile Ser

515 520 525

His Trp Leu Asn Thr His Ala Thr Ile Glu Pro Phe Val Ile Ala Thr

530 535 540

Asn Arg His Leu Ser Val Leu His Pro Ile His Lys Leu Leu Leu Pro

545 550 555 560

His Tyr Arg Asp Thr Met Asn Ile Asn Ala Leu Ala Arg Gln Ser Leu

565 570 575

Ile Asn Ala Asp Gly Val Ile Glu Arg Ser Phe Leu Pro Gly Lys Tyr

580 585 590

Ser Leu Glu Met Ser Ser Ala Val Tyr Lys Ser Trp Val Phe Thr Asp

595 600 605

Gln Ala Leu Pro Ala Asp Leu Ile Lys Arg Gly Met Ala Ile Glu Asp

610 615 620

Pro Cys Ala Pro His Gly Leu Arg Leu Val Ile Glu Asp Tyr Pro Tyr

625 630 635 640

Ala Val Asp Gly Leu Glu Ile Trp Asp Ala Ile Gln Thr Trp Val Lys

645 650 655

Asn Tyr Val Ser Leu Tyr Tyr Pro Thr Asp Asp Ala Ile Lys Lys Asp

660 665 670

Ser Glu Leu Gln Ala Trp Trp Lys Glu Ala Val Glu Thr Gly His Gly

675 680 685

Asp Leu Lys Asp Lys Pro Trp Trp Pro Lys Leu Asn Thr Pro Gln Asp

690 695 700

Leu Val His Ile Cys Ser Ile Ile Ile Trp Ile Ala Ser Ala Leu His

705 710 715 720

Ala Ala Val Asn Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Leu Asn

725 730 735

Arg Pro Thr Leu Thr Arg Arg Phe Leu Pro Glu Pro Gly Ser Lys Glu

740 745 750

Tyr Glu Glu Leu Ser Thr Asn Tyr Gln Lys Ala Tyr Leu Arg Thr Ile

755 760 765

Thr Arg Lys Ile Glu Ala Leu Val Asp Leu Ser Val Ile Glu Ile Leu

770 775 780

Ser Arg His Ala Ser Asp Glu Ile Tyr Leu Gly Lys Arg Asp Ser Asp

785 790 795 800

Asp Trp Thr Asp Asp Gln Lys Ala Ile Gln Ala Phe Glu Lys Phe Gly

805 810 815

Thr Lys Leu Lys Glu Ile Glu Ala Lys Ile Asn Ser Arg Asn Lys Asp

820 825 830

Ser Ser Leu Arg Asn Arg Asn Gly Pro Val Gln Met Pro Tyr Thr Val

835 840 845

Leu Leu Pro Thr Ser Glu Glu Gly Leu Thr Phe Arg Gly Ile Pro Asn

850 855 860

Ser Ile Ser Ile

865

<210> 104

<211> 860

<212> PRT

<213> 人工序列

<220>

<223> 脂加氧酶 Glyma08g20190.1 BLAST 860aa

<400> 104

Met Tyr Ser Gly Val Lys Gly Leu Phe Asn Arg Ser Gln Lys Val Lys

1 5 10 15

Gly Thr Val Val Leu Met Arg Lys Asn Val Leu Asp Ile Asn Ser Ile

20 25 30

Thr Ser Val Arg Gly Leu Ile Gly Thr Gly Ile Asn Ile Ile Gly Ser

35 40 45

Thr Ile Asp Gly Leu Thr Ser Phe Leu Gly Arg Ser Val Cys Leu Gln

50 55 60

Leu Ile Ser Ala Thr Lys Ala Asp Gly Asn Gly Asn Gly Val Val Gly

65 70 75 80

Lys Lys Thr Tyr Leu Glu Gly Ile Ile Thr Ser Ile Pro Thr Leu Gly

85 90 95

Ala Gly Gln Ser Ala Phe Thr Ile His Phe Glu Trp Asp Ala Asp Met

100 105 110

Gly Ile Pro Gly Ala Phe Leu Ile Lys Asn Tyr Met Gln Val Glu Leu

115 120 125

Phe Leu Val Ser Leu Thr Leu Glu Asp Ile Pro Asn Gln Gly Ser Met

130 135 140

His Phe Val Cys Asn Ser Trp Val Tyr Asn Ser Lys Val Tyr Glu Lys

145 150 155 160

Asp Arg Ile Phe Phe Ala Ser Glu Thr Tyr Val Pro Ser Glu Thr Pro

165 170 175

Gly Pro Leu Val Thr Tyr Arg Glu Ala Glu Leu Gln Ala Leu Arg Gly

180 185 190

Asn Gly Thr Gly Lys Arg Lys Glu Trp Asp Arg Val Tyr Asp Tyr Asp

195 200 205

Val Tyr Asn Asp Leu Gly Asn Pro Asp Ser Gly Glu Asn Phe Ala Arg

210 215 220

Pro Val Leu Gly Gly Ser Leu Thr His Pro Tyr Pro Arg Arg Gly Arg

225 230 235 240

Thr Gly Arg Lys Pro Thr Lys Lys Asp Pro Asn Ser Glu Lys Pro Gly

245 250 255

Glu Ala Tyr Ile Pro Arg Asp Glu Asn Phe Gly His Leu Lys Ser Ser

260 265 270

Asp Phe Leu Thr Tyr Gly Leu Lys Ser Leu Thr Arg Ser Phe Leu Pro

275 280 285

Ala Leu Lys Thr Val Phe Asp Ile Asn Phe Thr Pro Asn Glu Phe Asp

290 295 300

Ser Phe Glu Glu Val Arg Ala Leu Cys Glu Gly Gly Ile Lys Leu Pro

305 310 315 320

Thr Asp Ile Leu Ser Lys Ile Ser Pro Leu Pro Val Leu Lys Glu Ile

325 330 335

Phe Arg Thr Asp Gly Glu Ser Val Leu Lys Phe Ser Val Pro Asp Leu

340 345 350

Ile Lys Val Ser Lys Ser Ala Trp Met Thr Asp Glu Glu Phe Ala Arg

355 360 365

Glu Met Ile Ala Gly Val Asn Pro Cys Val Ile Arg Arg Leu Gln Glu

370 375 380

Phe Pro Pro Gln Ser Lys Leu Asp Pro Ser Val Tyr Gly Asp Gln Thr

385 390 395 400

Ser Lys Met Thr Ile Asp His Leu Glu Ile Asn Leu Glu Gly Leu Thr

405 410 415

Val Asp Lys Ala Ile Lys Asp Gln Arg Leu Phe Ile Leu Asp His His

420 425 430

Asp Thr Phe Met Pro Phe Leu Arg Arg Ile Asp Glu Ser Lys Ser Ser

435 440 445

Lys Ala Tyr Ala Thr Arg Thr Ile Leu Phe Leu Lys Asp Asp Gly Thr

450 455 460

Leu Lys Pro Leu Ala Ile Glu Leu Ser Leu Pro His Pro Gly Gln Gln

465 470 475 480

Gln Leu Gly Ala Tyr Ser Lys Val Ile Leu Pro Ala Asn Gln Gly Val

485 490 495

Glu Ser Thr Ile Trp Leu Leu Ala Lys Ala His Val Ile Val Asn Asp

500 505 510

Ser Cys Tyr His Gln Leu Ile Ser His Trp Leu Asn Thr His Ala Val

515 520 525

Ile Glu Pro Phe Val Ile Ala Thr Asn Arg Asn Leu Ser Ile Leu His

530 535 540

Pro Ile Tyr Lys Leu Leu Phe Pro His Tyr Arg Asp Thr Met Asn Ile

545 550 555 560

Asn Ala Leu Ala Arg Gln Ser Leu Ile Asn Ala Asp Gly Phe Ile Glu

565 570 575

Lys Thr Phe Leu Gly Gly Lys Tyr Ala Val Glu Ile Ser Ser Ser Gly

580 585 590

Tyr Lys Asn Trp Val Phe Leu Asp Gln Ala Leu Pro Ala Asp Leu Ile

595 600 605

Lys Arg Gly Met Ala Ile Glu Asp Ser Ser Cys Pro Asn Gly Leu Arg

610 615 620

Leu Val Ile Glu Asp Tyr Pro Tyr Ala Val Asp Gly Leu Glu Ile Trp

625 630 635 640

Asp Ala Ile Lys Thr Trp Val Gln Glu Tyr Val Ser Leu Tyr Tyr Ala

645 650 655

Thr Asn Asp Ala Ile Lys Lys Asp His Glu Leu Gln Ala Trp Trp Lys

660 665 670

Glu Val Val Glu Lys Gly His Gly Asp Leu Lys Asp Lys Pro Trp Trp

675 680 685

Pro Lys Met Gln Thr Leu Gln Glu Leu Ile Gln Ser Cys Ser Thr Ile

690 695 700

Ile Trp Ile Ala Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln Tyr

705 710 715 720

Pro Tyr Gly Gly Phe Ile Leu Asn Arg Pro Thr Leu Ser Arg Arg Trp

725 730 735

Ile Pro Glu Glu Gly Thr Pro Glu Tyr Asp Glu Met Thr Lys Asn Pro

740 745 750

Gln Lys Ala Tyr Leu Arg Thr Ile Thr Pro Lys Phe Gln Ala Leu Val

755 760 765

Asp Leu Ser Val Ile Glu Ile Leu Ser Arg His Ala Ser Asp Glu Val

770 775 780

Tyr Leu Gly Gln Arg Asp Asn Pro Asn Trp Thr Ser Asn Pro Lys Ala

785 790 795 800

Ile Glu Ala Phe Lys Lys Phe Gly Lys Lys Leu Ala Glu Ile Glu Thr

805 810 815

Lys Ile Ser Glu Arg Asn His Asp Pro Asn Leu Arg Asn Arg Thr Gly

820 825 830

Pro Ala Gln Leu Pro Tyr Thr Val Leu Leu Pro Thr Ser Glu Thr Gly

835 840 845

Leu Thr Phe Arg Gly Ile Pro Asn Ser Ile Ser Ile

850 855 860

<210> 105

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 105

Ser Ser Asp Phe Leu Thr Tyr Gly Ile Lys

1 5 10

<210> 106

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 106

Gly Thr Val Val Leu Met Pro Lys

1 5

<210> 107

<211> 13

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 107

Asn Val Leu Asp Phe Asn Ala Ile Thr Ser Ile Gly Lys

1 5 10

<210> 108

<211> 31

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 108

Gly Gly Val Ile Asp Thr Ala Thr Gly Ile Leu Gly Gln Gly Val Ser

1 5 10 15

Leu Val Gly Gly Val Ile Asp Thr Ala Thr Ser Phe Leu Gly Arg

20 25 30

<210> 109

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 109

Ile Phe Phe Val Asn Asp Thr Tyr Leu Pro Ser Ala Thr Pro Ala Pro

1 5 10 15

Leu Leu Lys

<210> 110

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 110

Asp Glu Asn Phe Gly His Leu Lys

1 5

<210> 111

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 111

Ser Leu Ser His Asp Val Ile Pro Leu Phe Lys

1 5 10

<210> 112

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 112

Ser Leu Tyr Glu Gly Gly Ile Lys

1 5

<210> 113

<211> 16

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 113

Thr Asp Gly Glu Asn Val Leu Gln Phe Pro Pro Pro His Val Ala Lys

1 5 10 15

<210> 114

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 114

Ile Asn Ser Leu Pro Thr Ala Lys

1 5

<210> 115

<211> 6

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 115

Thr Ile Leu Phe Leu Lys

1 5

<210> 116

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 116

His Leu Ser Val Leu His Pro Ile Tyr Lys

1 5 10

<210> 117

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 117

Gln Ser Leu Ile Asn Ala Asp Gly Ile Ile Glu Lys

1 5 10

<210> 118

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 118

Phe Ile Pro Ala Glu Gly Thr Pro Glu Tyr Asp Glu Met Val Lys

1 5 10 15

<210> 119

<211> 6

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 119

Ala Leu Glu Ala Phe Lys

1 5

<210> 120

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 人工序列

<400> 120

Gly Ile Pro Asn Ser Ile Ser Ile

1 5