控制昆虫虫害的方法

专利名称：控制昆虫虫害的方法
技术领域：
本发明涉及通过使用毒素蛋白，在农作物植株中控制鳞翅目秆野螟属种类，优选地Ostrinia furnacalis(亚洲玉米螟)的方法，毒素蛋白可从苏云金芽孢杆菌(Bt)和/或其它芽孢杆菌属种类得到。
苏云金芽孢杆菌属于革兰氏阳性大组，为需氧，形成内生孢子的细菌。与亲缘关系很近的其它芽孢杆菌属种类，如蜡状芽孢杆菌或炭疽芽孢杆菌不同，到目前为止已知的苏云金芽孢杆菌菌种大部分都在形成孢子时产生类芽孢内含体，由于它的晶体结构，一般称为晶体。该晶体含有具杀昆虫活性的晶体毒素原蛋白，即δ-内毒素。
苏云金芽孢杆菌对昆虫的毒性是由于该蛋白晶体。在口器摄入晶体，晶体溶于靶昆虫的肠液之前，δ-内毒素不表现其杀昆虫活性。在大多数情况下，由昆虫消化道的蛋白水解酶作用引起蛋白酶剪切，导致从毒素原释放有效毒性成分。
不同苏云金芽孢杆菌菌株的δ-内毒素，其特征在于对某些靶昆虫，尤其对应于不同的鳞翅目，鞘翅目和双翅目的幼虫有高度特异性以及针对这些易感幼虫有高度活性。苏云金芽孢杆菌δ-内毒素的进一步优点在于该毒素对人类，其它哺乳动物，鸟和鱼是无害的。
根据它们的活性范围和序列相似性，已将来自苏云金芽孢杆菌的不同杀昆虫晶体蛋白分类。按Hofte和Whiteley，微生物学综述53:242-255(1989)提出的分类将已知的杀昆虫晶体蛋白分成四大类。一般地，大类按它们的活性范围来分，CryⅠ蛋白对鳞翅目有效，CryⅡ蛋白对翅鳞目和双翅目都有效，CryⅢ蛋白对鞘翅目有效，CryⅣ蛋白对双翅目有效。在每一大类中，根据序列相似性将δ-内毒素分组。
CryⅠ蛋白典型地以130-140KDa毒素原蛋白合成，并经蛋白酶剪切产生大约60-70KDa大小的具杀昆虫活性的毒素蛋白。δ-内毒素的活性部分位于全长分子的氨基端部分。Hofte和Whiteley，同上将已知的CryⅠ蛋白分成六组，ⅠA(a),ⅠA(b),ⅠA(c),ⅠB,ⅠC和ⅠD。从那以后，分类命名为CryⅠE,CryⅠF,CryⅠG,CryⅠH和CryⅠX的蛋白也得到鉴定。
来自苏云金芽孢杆菌的每个δ-内毒素的杀昆虫活性范围趋于十分狭窄，所给的δ-内毒素只对一些昆虫有效。特异性是由于产生活性毒素蛋白涉及的各个步骤的效率和其随后与昆虫消化道上皮细胞相互作用的能力。
本发明的目的之一是提供在植物中控制Ostrinia furnacalis(亚洲玉米螟)种类的方法，所述植物优选农作物，包括，但不限于玉米，小麦，黑麦，燕麦，水稻，高梁，黍和相关作物，饲草，竹和甘蔗等种类。此目的可以在本发明的范围内，通过向要保护的作物植株施用苏云金芽孢杆菌的毒素蛋白如CryⅠ型毒素蛋白令人惊奇地得到实现。在本发明的另一个实施方案中，毒素蛋白可从芽孢杆菌属种类的无性培养物得到，所谓的无性杀昆虫蛋白(VIPs)，如VIP3[EP-A0690916；国际申请号EP95/03826，此处全文引用此公开文件作为参考]，也可以用于控制Ostrinia furnacalis(亚洲玉米螟)虫害。
因此，本发明涉及保护植物及其后代免受由Ostrinia furnacalis(亚洲玉米螟)种类引起的损害的方法，包括向所要保护的植物或植物种子或植物生长区域直接或间接施用纯的或者以至少含一种所述蛋白或微生物的杀昆虫组合物形式施用芽孢杆菌种类的毒素蛋白，优选地上述CryⅠ型或VIP型蛋白，上述微生物优选含至少一个编码毒素蛋白的毒素基因的苏云金芽孢杆菌和/或蜡状芽孢杆菌菌株。在根据本发明的方法中使用的上述微生物可以是或者天然存在的菌株，或者可选择地，含编码毒素的重组基因的重组菌株。
在优选的实施方案中，使用转基因植物给要保护的植物施用毒素以抵抗由Ostrinia furnacalis(亚洲玉米螟)引起的损害。这样的植物可通过转化编码芽孢杆菌属种类的杀昆虫毒素蛋白的毒素基因得到，上述杀昆虫毒素蛋白是比如Cry型，优选地CryⅠ型毒素蛋白或VIP型蛋白，并且在上述昆虫虫害出现的地区种植这样转化的植物时，转基因植物以足够提供针对Ostrinia furnacalis(亚洲玉米螟)种类的控制的量表达上述毒素蛋白。
在根据本发明的方法中使用的保护农作物植株以抵抗Ostriniafurnacalis(亚洲玉米螟)虫害的杀昆虫组合物，举例来说包含作为活性成分的至少一种cry型毒素蛋白，更优选地至少一种cryⅠ型毒素蛋白，甚至更优选的至少一种CryⅠA型毒素蛋白，尤其优选地至少一种CryⅠA(b)型毒素蛋白，尤其最优选地至少一种根据SEQ ID NOs53-55的cryⅠA(b)型毒素蛋白，甚至更优选地含至少一个编码上述毒素蛋白的基因的苏云金芽孢杆菌或微生物，优选地含至少一个编码上述毒素蛋白的基因的苏云金芽孢杆菌菌株，或其衍生物或突变株，结合农学上的佐剂如载体，稀释剂，表面活性剂或施用促进佐剂。在杀昆虫组合物中包含的活性成分也可以是在EP-A-0690916和PCT国际申请号EP95/03826中公开的VIP型毒素蛋白或CryⅠ型和VIP型蛋白结合。在保护的范围内优选的是VIPⅠ型蛋白，比如VIP1A(a)蛋白或VIP1A(b)蛋白，或VIP2型蛋白，比如VIP2A(a)蛋白或VIP2A(b)蛋白，或VIP1型蛋白和VIP2型蛋白的结合，或VIP3型蛋白，比如VIP3A(a)蛋白或VIP3A(b)蛋白。
在保护范围内更优选的是在SEQ ID NOs:l,2,4-7,17-24,26-32,35,36,39,40,42,43,45,46,49,50,5l或52中表示的VIP型毒素蛋白。
组合物也可以包含进一步的生物活性化合物。上述化合物可以是肥料或微量营养物供体或其他影响植物生长的制品。其也可以是选择的除草剂、杀昆虫剂，杀真菌剂，杀细菌剂，杀线虫剂、杀软体动物剂或几种这样制品的混合物，如果需要，可进一步与在配制技术中常规使用的农学上可接受的载体，表面活性剂或施用促进佐剂结合。适当的载体和佐剂可以是固体或液体，并对应配制技术中一般使用的物质，例如天然的或再生的矿物质，溶剂，分散剂，润湿剂，粘着剂，粘合剂或肥料。
按重量比，组合物可以包括0.1-99％的活性成分，1-99.9％的固体或液体佐剂，和0-25％表面活性剂。活性成分或含上述活性成分的组合物可以与某一其它杀昆虫剂或化学品(1993农作物保护化学品参考手册，化学品和药品出版社，加拿大)结合施用给要保护植物或农作物而不失去效力。其可以与大多数其它通用的农业喷洒物质相容，但如果涉及CryⅠ型毒素，则不应该在强碱性喷洒溶液中使用。其可以以粉末，悬浮液，可湿性粉末或任何对农业应用适当的其它物质形式施用。
活性成分，即优选地苏云金芽孢杆菌的CryⅠ型毒素蛋白和/或前述的任一VIP型蛋白，或含上述活性成分的组合物可以施用给(a)可能有昆虫虫害的环境，(b)植物或植物的一部分，为了保护该植物或植物的一部分免受昆虫虫害引起损害，或(c)种子，为了保护从该种子长出的植株免受虫害的损害。
在植物保护区域内施用的优选方法是向植物的叶施用(叶敷)，施用的数量和施用的频率根据要保护的植物种类和受该虫害侵袭的危险度。
在根据本发明的方法中使用的组合物也适于保护植物繁殖材料，例如种子，比如果实，块茎或籽粒，或植物插条，免受昆虫虫害。可以在种植前用配制品处理繁殖材料，举例来说，种子可以在播种前拌药。本发明的活性成分也可以通过用液体配制品浸泡籽粒或者通过用固体配制品覆盖籽粒施用给籽粒(涂盖层)。配制物也可以在种植繁殖材料时，施用在种植位置，例如播种时的种子梨沟。本发明也涉及处理植物繁殖材料的这些方法和这样处理的植物繁殖材料。
在本发明的范围内，组合物可以按任何已知的用细菌菌株处理种子或土壤的方法施用。例如，见美国专利号4,863,866。菌株即使在微生物不存活的情况也对生物控制有效。然而优选地，施用存活的微生物。
在本发明的范围内所要保护的靶农作物是那些Ostrinia furnacalis(亚洲玉米螟)的宿主植物，包括但不限于玉米、小麦、大麦、黑麦、燕麦、水稻、高梁、黍和相关农作物，饲草、竹和甘蔗。
根据本发明的活性成分可以以未修饰的形式或与任何适当的农业上可接受的载体结合使用。这样的载体是在农业配方技术中常规使用的佐剂，并且由此按已知方法配制或可乳化的浓缩物，可覆盖膏剂，直接可喷洒的或可溶的溶液，稀释乳剂，可湿性粉末，可溶粉末，药粉，颗粒，以及胶囊，例如以聚合物形式。正如组合物的性质，施用方法，如喷洒、喷雾、撒粉、分散或浇灌，也可根据预期目的和普遍情况选择。有效施用的比率从每公顷(“ha”大约2.471英亩)大约50g到大约5kg活性成分(a.i.)之间变动，优选地从大约100g到大约2kg a.i./ha。施用的优选比率是200g到大约1kg a.i./ha)或200g-500g a.i./ha。
对于拌种，有效施用的比率从每100kg种子0.5g到1000ga.i.之间变动，优选地从每100kg种子3g到100g a.i.。最优选的是施用比率从每100kg种子10g到50g a.i.。
适当载体和佐剂可以是固体或液体，并对应在配制技术中一般使用的物质，例如天然或再生的矿物质，溶剂，分散剂，润湿剂，粘着剂，粘合剂或肥料。配制物，即杀昆虫组合物，制品或其与其它活性成分和适当的固体或液体佐剂的混合物，可按已知的方法制备，例如通过将活性成分与填料，例如溶剂，固体载体和某些情况下的表面活性化合物(表面活性剂)均匀混合和/或研磨。
合适的溶剂是芳香烃，优选地含8-12个碳原子的组分，例如二甲苯混合物或取代的萘，邻苯二甲酸酯如邻苯二甲酸二丁酯或邻苯二甲酸二辛酯，脂族烃如环己烷或链烷烃，醇和二元醇和它们的醚和酯，如乙醇，1,2-亚乙基二醇单甲基或单乙基醚，酮如环己酮，强极性溶剂如N-甲基-2-吡咯烷酮，二甲基亚枫或二甲基甲酰胺，和植物油或环氧化植物油如环氧化椰子油或豆油；或水。
所用的固体载体，例如对于药粉和可分散粉末，一般是天然矿物填料如方解虫，滑石粉，高岭土，蒙脱土或硅镁土。为了提高物理特性，也可能加高度分散的硅酸或高度分散的吸附剂聚合物。适当的颗粒吸附载体是多孔型，例如浮石，碎砖土，海泡石或皂土；适当的非吸附载体是诸如方解石或砂的材料。另外大量的具无机或有机性质的预先形成颗粒的材料也可使用，例如，尤其是白云石或粉状植物残渣。
根据要配制的活性成分的性质，适当的表面活性化合物是有很好乳化，分散和湿润性质的非离子，阳离子和/或阴离子表面活性剂。术语“表面活性剂”也将理解为包括表面活性剂混合物。适当的阴离子表面活性剂可以是水溶的肥皂和水溶的合成的表面活性化合物。适当的肥皂是高级脂肪酸的碱金属盐，碱土金属盐或未取代的或取代的铵盐(C10-C22)，例如，油酸或硬脂酸的钠盐或钾盐，或例如，可从椰子油或牛羊油得到的天然脂肪酸混合物。更适当的表面活性剂也可是脂肪酸甲基氨基乙磺酸盐及修饰的和未修饰的磷脂。
然而，更经常地，使用所谓的合成的表面活性剂，尤其是脂肪族磺酸盐，脂肪族硫酸盐，磺化苯并咪唑衍生物或烷芳基磺酸盐。脂肪族磺酸盐或硫酸盐常常以碱金属盐，碱土金属盐或未取代的或取代的铵盐形式，并且一般含有C8-C22烷基，也包括酰基的烷基组分，例如木素磺化酸和十二烷基硫酸的钠盐或钙盐；或可从天然脂肪酸得到的脂肪族醇硫酸盐的混合物。这些化合物包括脂肪族醇/环氧乙烷加合物的硫酸盐和磺酸盐。磺化苯并咪唑衍生物优选地含2个磺酸基团和一个含大约8-22个碳原子的脂肪酸基团。烷芳基磺酸盐的例子是十二烷基苯磺酸，二丁基萘磺酸或萘磺酸/甲醛缩合产物的钠盐，钙盐或三乙醇胺盐。相应的磷酸盐也是适合的，例如对-壬基酚与4-14摩尔环氧乙烷的加合物的磷酸酯盐。非离子表面活性剂优选地是脂族醇或环脂族醇的聚乙二醇醚衍生物，或饱和的或非饱和的脂肪酸和烷基酚，上述衍生物在脂肪烃组分中含3-30个乙二醇醚基团和8-20个碳原子中并在烷基酚的烷基组分中含6-18个碳原子。
更适当的非离子表面活性剂是聚环氧乙烷与在烷基链中含1-10个碳原子的聚丙二醇，乙二胺聚丙二醇和烷基聚丙二醇的水溶加合物，该加合物含20-250个乙二醇醚基团和10-100丙二醇醚基团。这些化合物每个丙二醇单位通常含1-5个乙二醇单位。非离子表面活性剂的代表性的例子是壬基苯基聚乙氧基乙醇，蓖麻油聚乙二醇醚，聚环氧丙烷/环氧乙烷加合物，三丁基苯氧基聚乙氧基乙醇，聚乙二醇和癸基苯氧基聚乙氧基乙醇。聚氧化乙烯山梨聚糖的脂肪酸酯，如聚氧化乙烯山梨聚糖三油酸酯，也是合适的非离子去污剂。
阳离子去污剂优选地是季胺盐，包括如N-取代基，至少一个C8-C22烷基，及进一步取代基，低级的非取代的或卤代的烷基，苄基或羟基-低级烷基。该盐优选地以卤化物，硫酸二甲酯或硫酸二乙酯的形式，例如氯化硬脂酰三甲基胺或溴化苄基二-(2-氯乙基)乙基铵。
在配制技术中习惯使用的表面活性剂在例如以下文献中描述“McCutcheon的去污剂和乳化剂年鉴”MC出版公司。Ridgewood,N.J.,1979；Dr.Helmut Stache,“Tensid Taschenbuch”(表面活性剂手册)，Carl Hanser Verlag,Munich/vienna。
本发明的杀昆虫组合物另一特别优选的特征是当施用于植物和土壤时活性成分的持久性。引起活性丧失的可能原因包括由于紫外光，热，叶分泌物和PH的失活。例如，在高PH，尤其存在还原剂时，δ-内毒素晶体溶解，从而变得更容易蛋白水解失活。叶的高PH值也可能是重要的，特别是在叶表面PH可以在8-10的范围内的地方。在根据本发明的方法中使用的杀昆虫组合物的配方可以通过加入添加剂帮助防止活性成分的丧失或把物质包在胶囊内，以此方法保护活性成分免于失活来解决这些问题。胶囊化可以用化学方法McGuire和Shasha,J Econ Entomol85:1425-1433,1992)或生物方法(Barnes和Cunmings,1986；EP-A0192319)进行。化学胶囊化涉及用聚合物包裹活性成分的步骤，而生物胶囊化涉及在在微生物中表达δ-内毒素基因，对于生物胶囊化，在配方中使用包含毒素蛋白的完整的微生物作为活性成分。加紫外线防护剂可以有效地减少辐射损伤。由于热引起的失活也可以通过加入合适的添加剂控制。
如果可能，在本发明中优选的是包括存活的微生物作为活性成分的配方，上述微生物或者以无性细胞的形式，或者更优选地以孢子的形式。适当的配方可以包含，例如与多价阴离子交联的聚合物胶，并包含这些微生物。例如由D.R.Frarel等在植物病理学，Vol.75,No.7,774-777,1985中描述藻酸盐作为聚合物材料。从此公开文本中也可知道载体材料可以一起被使用。这些配方可以按以下方式制备通过将天然存在或合成的成胶聚合物，例如藻酸盐和多价金属离子盐水溶液混合，以形成单个液滴，从而对于微生物，有可能悬浮在两个反应溶液之一或在两个反应溶液中。胶的形成从以液滴形式混合开始。随后可能是这些胶颗粒的干燥。这个过程称为离子胶凝作用。根据干燥程度，形成了紧密和坚硬的聚合物颗粒，其在结构上通过多价阳离子交联，并包含普遍均匀分布的微生物和载体。颗粒大小可以长达5mm。
在EP-A1-0097571中描述了以部分交联的多聚糖及微生物为基础的组合物，其例如也可以包含精细分开的硅酸作为载体材料；例如通过Ca++离子发生交联。该组合物具有不超过0.3的水活度。W.J.Cornick等在综述文章[生物控制中的新动向抑制农业上虫害和疾病的改进方法，345-372页，Alan R.Liss,Inc.(1990)]中描述了不同的配方系统，用蛭石作为载体的颗粒和通过所述的离子胶凝作用方法制备的紧密的藻酸盐珠粒。在杀昆虫配方和应用系统中这样的组合物也由D.R.Fravel公开11卷，ASTM STP1112美国测试和材料学会，Philadelphia,1992,173-179页，并可以用来配制根据本发明的重组微生物。为配制存活微生物的进一步的方法在WO96/02638中描述。
根据本发明的组合物即使在低的施用比率时，也对虫害控制领域的预防性和/或治疗性处理有益，同时它对于温血种类，鱼和植物是耐受的和无毒性的，并具有很适当的杀生物剂范围。根据本发明的组合物可有效地抵抗Ostrinia furnacalis(亚洲玉米螟)虫害的所有或单独发育阶段。根据本发明化合物的杀昆虫作用可以直接显现出来，即立即消灭虫害，或者仅在过了一段时间之后显现。
上述组合物可以以包含基本上纯的毒素蛋白的化学混合物的形式或以包含至少一种作为微生物或转基因植物的一部分的毒素蛋白的混合物形式提供。
在本发明特定的实施方案中，活性成分之一可以直接地通过，例如如本文前述的叶面喷洒施用给植物，而另一活性原理可以由植物本身通过表达事先转化的编码上述另一原理的基因提供。
在根据本发明的方法中使用的杀昆虫组合物通常含从大约0.1％到大约99％，优选地从大约0.1至大约95％，最优选从大约3至90％的活性成分；从大约1至大约99.9％，优选地从大约1至大约99％，最优选地从大约5到大约95％的固体或液体佐剂；和从大约0至大约25％，优选地大约0.1到大约25％，最优选地从大约0.1到大约20％的表面活性剂。
同时商业产品优选地配制成浓缩物，而最终使用者一般将使用实际上更低浓度的稀释配制液，杀昆虫组合物也可能包含进一步成分，比如稳定剂，消泡剂，粘度调节剂，粘合剂，粘着剂，肥料或其它活性成分以得到特殊的效果。
本发明也涉及包含存活微生物为活性成分的配方，如果可能，上述微生物以无性细胞或更多地以孢子形式存在。
本发明的进一步目的涉及在控制农作物植株免受由Ostriniafurnacalis(亚洲玉米螟)种类引起的损害的方法中重组微生物的应用。该重组微生物含编码苏云金芽孢杆菌毒素蛋白如CryⅠ型蛋白的毒素基因，该重组微生物或者直接施用给要保护的植物，或者重组合成的毒素蛋白在施用给要保护的农作物植株前先从重组微生物分离并如上述所配制。重组微生物也可以包含编码在EP-A-690916和国际申请号NOEP95/03826中公开的VIP型毒素蛋白的毒素基因或者分别编码至少一种Cry型毒素和VIP型毒素的基因的组合。
为了在宿主生物中重组合成毒素蛋白，编码序列可以插入到为所选宿主设计的表达元件中并导入宿主，在宿主中重组合成。选择适合于所选宿主的特异调节序列如启动子，信号序列，5’和3’非翻译序列，和增强子在本领域从业者的技术水平之内。所得的分子，包含了适当的阅读框架中连接的特定元件，插入到能够转化到宿主细胞中的载体中。对于各种宿主生物适合的表达载体和重组合成蛋白的方法已众所周知。上述宿主生物包括如大肠杆菌[见，例如Studier and Moffatt，分子生物学杂志189:113(1986)；Brosius,DNA 8:759(1989)]，酵母[见，例如Schneiderand Guarente，酶学方法194:373(1991)]和昆虫细胞[见，例如，Luckow and Summers，生物/生物技术6:47(1988)]。特异的例子包括质粒如pBluescript(Stratagene,La Jolla,CA),pFLAG(International Biotechnologies,Inc.,New Haven,CT),pTrcHis(Invitrogen,La Jolla,CA)和杆状病毒表达载体，例如那些从苜蓿丫纹夜蛾核型多角体病毒基因组派生的载体。优选的杆状病毒/昆虫系统是PVI11392/SF21细胞(Invitrogen,La Jolla,CA)。
重组合成的毒素蛋白可以使用一系列标准技术分离并纯化。可以使用的有效技术将根据所用的宿主生物，毒素蛋白是否设计为分泌，和其它本领域技术人员意识到的这样的因素变动[见，例如Ausubel,F.等“分子生物学常规技术”，由John Wiley & Sons公司出版，(1994)第16章]。
本发明优选的目的涉及在保护农作物植株免受由Ostrinia furnacalis(亚洲玉米螟)虫害引起的损害的方法中转基因植物的应用，该转基因植物包含并以足以提供针对Ostrinia furnacalis(亚洲玉米螟)种类的控制的量表达编码苏云金芽孢杆菌的毒素蛋白，特别地CryⅠ型毒素蛋白的毒素基因。植物可以是核转化或质粒转化的结果(见WO95/24492)。特别优选的是表达苏云金芽孢杆菌的CryⅠA(6)毒素蛋白的转基因植物。本发明也涉及包含编码如在EP-A-690916和国际申请号EP95/03826(此处全文引用作为参考)中描述的VIP型蛋白的毒素基因的转基因植物的应用。本发明也涉及包含和表达编码苏云金芽孢杆菌的毒素蛋白，但尤其是Cry型毒素蛋白的毒素基因的，和也包含并足够提供针对Ostriniafurnacalis(亚洲玉米螟)种类的控制的量表达编码VIP型蛋白的毒性基因的转基因植物的应用。表达上述毒素基因的宿主植物将具有增强的对Ostrinia furnacalis(亚洲玉米螟)种类昆虫侵袭的抗性并将更好地减少与这种侵袭相关的农作物损失。
在一个优选的实施方案中，在转基因植物中表达一种或多种Bt δ-内毒素伴随表达一种或多种VIP型蛋白。在同一转基因植物中共表达多于一种杀昆虫原理可以通过遗传操作植物以包含并表达所有必须基因实现。可选择地，一种植物，亲本1，可以经遗传工程操作以表达VIP型蛋白。另一植物，亲本2，可以经遗传工程操作以表达Bt δ-内毒素。通过亲本1和亲本2的杂交，得到的子代表达所有导入亲本1和亲本2的基因。特别优选的Bt δ-内毒素是在EP-A0618976中公开的那些，此处引用作为参考。
本发明包括含编码能在中度严格条件下与编码芽孢杆菌属种类毒素蛋白的DNA分子，但优选地与可从上述DNA分子得到的寡核苷酸探针杂交的DNA分子的基因的重组微生物或转基因植物的应用，其中上述探针长至少10个核苷酸包含上述毒素蛋白的编码序列的连续部分。本发明优选地包括编码与编码苏云金芽孢杆菌或蜡状芽孢杆菌的毒性蛋白的DNA分子，特别地与编码Cry型蛋白的DNA分子或编码VIP型毒素蛋白，优选地CryⅠA(b)蛋白的毒素基因杂交的DNA分子的基因的重组微生物或转基因植物的应用。
影响杂交稳定性的因素决定杂交的严格度。这样的因素之一是解链温度Tm,Tm可以容易根据公式得到计算，公式在DNA探针，GeorgeH.Keller和Mark M.Manak,Macmillan Publisher Ltd,1993,第一部分分子杂交技术；第8页中提供。
优选的杂交温度在低于计算解链温度Tm大约25℃的范围内，并且优选地在低于计算解链温度Tm大约12-15℃的范围内，并且在寡核苷酸的情况下在低于解链温度Tm大约5-10℃的范围内。
本发明进一步涉及用于在农作物植株中控制Ostrinia furnacalis(亚洲玉米螟)虫害，包含转基因植物种子及可靠的使用说明的商品包，上述转基因植物包含至少一个毒素基因，并以足以提供针对Ostriniafurnacalis(亚洲玉米螟)种类的控制的量表达该毒素基因，该毒素基因编码苏云金芽孢杆菌的毒素蛋白，优选地Cry型毒素蛋白，更优选地CryⅠ型毒素蛋白，但最优选地CryⅠA型毒素蛋白。在本发明内优选的是包括包含编码至少一个Cry型毒素蛋白或VIP型毒素蛋白的基因作为活性成分的转基因植物种子的商品包。特别优选地是CryⅠA(b)毒素蛋白与VIP型蛋白的组合。
本发明进一步的目的是用于在农作物植株中控制Ostrinia furnacalis(亚洲玉米螟)虫害，包括根据本发明的杀昆虫组合物及其可靠的使用说明的商品包。
对于植物，是指任何可以通过本领域已知的方法进行基因转化的植物种类，但尤其是Ostrinia furnacalis(亚洲玉米螟)种类的宿主植物的那些植物，包括，但不限于以下植物种类玉米、小麦、大麦、黑麦、燕麦、水稻、高梁，黍和相关作物，饲草、竹(果园草，羊茅，等等)，和甘蔗。
在本领域已知的植物转化的方法在下面讨论。作为目的作物的宿主植物包括，但不限于以上列出的那些种类。
本发明进一步涉及包含编码苏云金芽孢杆菌的毒素蛋白的基因并以足够提供针对Ostrinia furnacalis(亚洲玉米螟)种类的控制的量表达该毒素蛋白的转基因植物的种子，以及包括该种子的商品包。
对于植物，是指是Ostrinia furnacalis(亚洲玉米螟)宿主的任何植物种类，包括，但不限于玉米、小麦、大麦、黑麦、燕麦、水稻、高梁、黍和相关作物，饲草，竹和甘蔗。
已发现天然苏云金芽孢杆菌毒素基因的密码用法与植物基因的典型用法明显不同。特别地，天然苏云金芽孢杆菌基因的密码子用法与玉米基因的非常不同。因此，这个基因mRNA可能不能有效的利用。密码子用法可能在翻译或转录或mNRA加工水平上影响基因的表达。为使毒素蛋白在植物中，例如在玉米中最佳表达，通过在合成编码与天然毒素基因序列编码的相同的蛋白的合成基因中使用在玉米中最优选的密码子使密码子用法最佳化。最佳化的优选的玉米密码子用法对高水平表达Bt杀昆虫蛋白有效。构建玉米最佳化的合成的毒素基的进一步细节可在WO/3/07278中得到，此处全文引用作为参考。
来自微生物的毒素基因也可能与植物基因不同。植物基因与微生物中发现的基因不同之处在于，它们转录的RNA不产生邻近起始甲硫氨酸的特定的核糖体结合位点序列。所以，微生物基因可以通过在ATG处插入真核生物保守的翻译起始区加强表达。Clontech(1993/1994目录，210页)建议序列GTCGACCATGGTC作为在植物中表达大肠杆菌uidA基因的保守的翻译起始区。另外，Joshi[核酸研究15:6643-6653(1987)]比较了一些邻近ATG的植物序列并证明了保守区TAAACAATGGCT。在植物中表达微生物ORFs遇到困难的情况下，在起始ATG处插入一个这样的序列可能改进翻译。由于第二个氨基酸残基的修饰，在这种情况下不适合将保守区的后三个核苷酸包含在修饰序列中。优选的邻近起始甲硫氨酸的序列可能在不同的植物种类之间不同。按在GenBank/EMBL数据库中提供的玉米基因的序列，可以发现哪些邻近ATG的核苷酸应该被修饰以增强转化入玉米中的毒素基因的翻译。
另外，已表明去除不合理剪切位点可以增强导入基因的表达和稳定性。从非植物来源克隆的，在植物中不适于表达的基因可能包括一些模式，这些模式能在植物中被识别为5＇或3＇剪切位点。因而，转录过程可能未成熟中止，产生截短的或缺失的mRNA。毒素基因可以被加工以去除这些不合理剪切位点。
一些苏云金芽孢杆菌的δ-内毒素蛋白表达为毒素原。这些毒素原在昆虫胃的碱性环境中溶解并由蛋白水解酶剪切成有毒核心片段[Hofte和whiteley，微生物综述，53:242-245(1989)]。对于CryⅠ类的δ-内毒素蛋白，有毒核心片段位于毒素原的N端部分。在带给宿主植物杀昆虫特性的植物转化载体中使用的编码或者全长毒素原形式或完整毒素蛋白截短的有毒核心片段的基因都在本发明的范围之内。
重组DNA分子可以用很多本领域公认的方法导入植物细胞。本领域技术人员将同意，方法的选择可能依赖于要转化的靶植物类型，即单子叶或双子叶。转化植物细胞的适当方法包括微注射[Crossway等，生物技术4:320-334(1986)]，电穿孔[Riggs等，美国国家科学院院报83:5602-5606(1986)]，土壤农杆菌介导的转化[Hinchee等，生物技术6:915-921(1988)]，直接基因转化[Paszkowski等，EMBO J.3:2717-2722(1984)]，和弹粒加速，使用从Agracetus,Inc.,Madison,Wisconsin和Dupont,Inc.,Wilmington,Delaware可得到的仪器[见，例如，Sanford等，美国专利4,945,050；和McCabe等，生物技术6:923-926(1988)]。也见，Weissinger等，遗传学年鉴22:421-477(1988)；Sanford等，微粒科学和技术5:27-379(1987)(离子)；Christou等，植物生理学87:671-674(1988)(大豆)；McCabe等，生物/生物技术6:923-926(1988)(大豆)；Datta等，生物/生物技术8:736-740(1990)(水稻)；Klein等，美国国家科学院院报，85:4305-4309(1988)(玉米)；Klein等，生物/生物技术6:559-563(1988)(玉米)；Klein等，植物生理学91:440-444(1988)(玉米)；Fromm等，生物/生物技术8:833-839(1990)；和Gordon-Kamm等，植物细胞2:603-618(1990)(玉米)；Svab等，美国国家科学院院报87:8526-8530(1990)(烟草原生质体)；Koziel等，生物技术11:194-200(1993)(maize)；Shimamoto等，自然338:274-277(1989)(水稻)；Christou等，生物技术9:957-962(1991)(水稻)；欧洲专利申请EP0332581(果园草和其它Pooideae)；Vasil等，生物技术11:1553-1558(1993)(小麦)；Weeks等，植物生理学102:1077-1084(1993)(小麦)；Wan等，植物生理学104:37-48(1994)(大麦)；Umbeck等，生物/生物技术5:263-266(1987)(棉花)。
特别优选的通过微粒轰击将重组DNA分子导入玉米的一系列实施方案可在WO93/07278中找到，此处全文引用作为参考。另一优选的实施方案是在申请EP-A-292435中公开的玉米的原生质体转化方法，此处全文引用作为参考。
上述导入转基因种子和植物的基因特性通过性繁殖或无性生长传代，因此也可以在子代植物中维持和繁殖。一般地上述维持和繁殖使用已知用于适应特定目的如耕地，播种，或收获的农业方法。也可以使用特殊的方法如水载培或温室技术。因为正在生长的农作物易于受到昆虫或侵染的侵袭和损害及杂草植物的竞争，采取控制杂草，植物疾病，昆虫，线虫，和其它不良条件的办法以提高产量。这些包括机械方法如耕耘土壤或除去杂草和侵染的植物，及使用农业化学品如除草剂，杀真菌剂，杀配子剂，杀线虫剂，生长调节剂，催热剂和杀昆虫剂。
根据本发明的转基因植物和种子的有益遗传特性可进一步在植物育种中使用，遗传育种目的在于发展具改良特性的植物，如对虫害，除草剂或逆境的耐受性，改进营养价值，增加产量，或改进结构减少倒伏。各种育种步骤以良好管理的人类干预为特征，如选择要杂交的株系，介导亲本株系的传粉，或选择适当的子代植株。根据所需特征，采用不同的育种方法。相关技术在本领域内已知，包括但不限于杂交，近亲交配，回交育种，多系育种，多种混合，种间杂交，非整倍体技术等。杂交技术也包括通过机械，化学或生化方法，使植物不育以产生雄性或雌性不育植株。雄性不育植株与不同株系的花粉交叉授粉确保了雄性不育植株的基因组，而雌性可育植物将均等得到两个亲本株系的特征。这样，根据本发明的转基因种子和植物可以用于培育改良的植物株系，该株系例如提高常规方法如除草剂或杀虫剂处理的效率，或由于它们的改良的遗传特征用上述方法推广。可选择地，可以得到具改进的逆境抗性的新作物，由于它们最佳化的遗传“元件”，产出比不能耐受相应不良发育条件的产品更高品质的收获产品。
种子生产中，种子的发芽质量和均一性是必须的产品特性，而被农民收获和售出的种子的发芽质量和均一性并不重要。因为很难控制作物不受其他作物和杂草种子的影响，为了控制种生疾病和生产良好发芽的种子，种子生产者已发展了相当广泛和很好管理的种子生产方法，而种子生产者在生长，调控和市场销售纯种的领域内有经验。常用方法是农民购买满足特定品质标准的已检测的种子，而不是使用从自己的作物收获的种子；使用的繁殖材料如种子，常规地用保护性涂层处理，保护性涂层包括除草剂，杀昆虫剂，杀真菌剂，杀细菌剂，杀线虫剂，杀配子剂或它们的混合物。常规使用的保护性涂层包括诸如克菌丹，莠锈灵，福美双(TMTD),methalaxyl(Apron)和；虫螨磷(Actellic)的化合物。如果需要，这些化合物进一步与其它在配制技术中常规使用的载体，表面活性剂或施用促进佐剂一起配制以提供保护抵抗细菌、真菌或动物虫害引起的损害。保护性涂层可以通过用液体配制物浸渍繁殖材料或通过用组合的湿或干的配制物涂敷施用。其它施用方法也是可能的，如直接在花芽或果实上处理。
本发明的另一方面是提供新的农业方法，如以上列举的方法，此方法的特征在于使用根据本发明的转基因植物，转基因植物材料，或转基因种子以提供针对Ostrinia furnacalis(亚洲玉米螟)的控制。
为从根据本发明的方法转化的植株繁育子代，可以使用如以下的方法如本领域已知的，如在下述实施例中描述的得到的玉米植物在温室的盆中或在土壤中生长，并使之开花。从成熟雄花穗得到花粉，并用于给同一植株，同胞植株或任何所选的玉米植株的雌花穗传粉。按此方法得到的转化子代可以通过导入基因和/或伴随DNA(基因型)的存在，或带来的表现型与未转化的子代区别开。转化子代可以相似地自交或与其它植物杂交，一般与带有所需品质的任意植物杂交。相似地，烟草或其它按此方法得到转化植物可以如在本领域已知的自交或杂交，以产生具有所需特性的子代。相似地，通过结合本领域已知的方法和本发明产生的其它转基因植物可以如在本领域已知的培育以产生具所需特征的子代。
实施例以下实施例进一步描述了为完成本发明的特定实施方案使用的材料和方法。它们通过说明的方法提供，并不应理解为限制本说明书的公开。实施例1一般方法DMA操作使用在本领域常规应用的方法进行。这些方法可以在基本上不改变结果的情况下修饰和/或改变。除了其它已说明的文献，一些方法在常用的书本如Sambrook等，分子克隆实验手册，冷泉港实验室出版社，第二版，1989中描述。实施例2植物转化载体植物转化使用在WO93/07278和Koziel等(1993)[生物技术Vol11 194-200]中描述的转化载体PCIB4431和PCIB3064进行，两个公开文献在此处引用作为参考。
PCIB4431是设计以转化玉米的载体。它包含两个嵌合的合成的在玉米能表达的Bt CryⅠA(b)内毒素基因，其中之一构建了PEP羧化酶启动子/合成的CryⅠA(b)基因，另一为花粉启动子/合成的CryⅠA(b)基因。
PCIB4431包含了在SEQ ID NO:1中提供的合成的CryⅠA(b)基因，并于1992年9月21日保存于农业研究机构保藏中心(NRRL)，北部区域研究中心，1815 North University Street,Peoria,Illinois61604,U.S.A，保藏号为NRRL B-18998。
PCIB3064包含植物可表达的bar基因(615bP)，原始地从吸水链霉菌克隆[Thompson等(1987)EMBO J 6,2519-2523]。它编码了磷丝菌素酰基转移酶(PAT)，带来对磷丝菌素的抗性，bar基因在CaMV 35S启动子和终止子的控制下[OW等(1987)美国国家科学院院报84,4870-4874]以提供对磷丝菌素的抗性。实施例3含合成的玉米CryⅠA(b)基因的转基因玉米植株的产生。
以下实施例利用Biolistic仪器将DNA包被的颗粒导入玉米细胞，从中产生转化的植株。3.1组织未成熟玉米胚，大约长1.5-2.5mm,在传粉后14-15天，从基因型6N6 15的雌穗切出，母株在温室中生长。在切除前，雌穗用20％Cloxox表面消毒20分钟，并用无菌水冲洗三次。单个胚盾片朝上放在2平方厘米区域内，每盘36个胚，并置于愈伤诱导培养基，2DG4+5草灭平培养[N6大量盐，B5微量盐，MS铁离子，2％蔗糖和5mg/l草灭平，20mg/l葡萄糖，以及在高压灭菌后加10mlG4添加剂(表1)]上。表1G4添加剂成分 每升培养基酪蛋白水解物0.5g脯氨酸 1.38g尼克酸 0.2mg吡哆素-HCl 0.2mg硫胺素-HCl 0.5mg胆碱-HCl0.1mg核黄素 0.05mg生物素 0.1mg叶酸0.05mg泛酸钙 0.1mg对氨基苯甲酸0.05mgB12 0.136μg3.2用于转化的DNA的制备微载体根据与Biolistic仪器一起提供的说明书制备，在震荡50μl1.0μm金微载体的同时，加入5μl pCIB4431(1.23μg/μl)[#898]+2μl PCIB3064(0.895mg/μl)[#456]，再加入50μl 2.5MCaCl2，然后加入20μl 0.1M亚精胺(自由基，TC级)。得到的混合物震荡混匀3分钟并离心10秒。去除上清，并用250μl 100％乙醇(HPLC级)通过短暂震荡，离心并去除上清冲洗微载体2次，微载体重悬于65μl 100％乙醇中。3.3轰击使用PDS-1000He Biolistic仪器轰击组织。组织放置于制动筛选挡板(stopping screen shelf)以下8cm的格子上，用10μl DNA/金微载体溶液射击组织一次，并在大载体(macrocarrier)上干燥。所用的终止筛板使用10×10不锈钢筛目手动冲压，使用1550psi值的自裂片。轰击后，胚在黑暗中，25℃培养。3.4愈伤形成轰击1天后，胚转移到含3mg/l PPT的愈伤诱导培养基上。轰击后2和3周评估胚的愈伤形成。所有有反应的胚转移到愈伤维持培养基，含3mg/L PPT的2DG4+0.5 2,4-D培养基上。愈伤维持培养基是N6大量盐，B5微量盐，MS铁，2％蔗糖，加0.5mg/L 2,4-D,20mg/l葡萄糖，并在高压灭菌后加入10ml G4添加剂。胚生愈伤每隔2周用含3mg/L PPT的新鲜维持培养基传代，所有愈伤组织在黑暗中于25℃温育。
Ⅰ型愈伤形成反应是15％。每一个产生愈伤的胚作为单独情况培养，以形成单独株系。3.5再生筛选12周后，将组织从含PPT的愈伤维持培养基上移到再生培养基上。再生培养基是0.25MS3S5BA(0.25mg/L 2,4-D,5mg/L BAP,MS盐类，3％蔗糖)，2周后，在MS3S培养基上再培养以再生植株。5到10周后，取出植物，并放入GA7＇S中。实施例4转基因玉米植株的分析4.1 ELISA分析在转基因玉米中CryⅠA(b)基因的表达检测使用亚洲玉米螟昆虫生物分析和ELISA分析监控，以从数量上确定得到的CryⅠA(b)蛋白的水平。
使用酶联免疫吸附分析(ELISA)对转基因植物叶中CryⅠA(b)杀昆虫蛋白进行数量上的测定，如在Clark MF,Lister RM,Bar-Joseph M:ELISA技术。于Weissbach A,Weissbaeh H(编辑)酶学方法118:742-766，学术出版社，Florida(1986)公开。使用免疫亲合纯化的对苏云金芽孢杆菌库尔斯塔克亚种的杀昆虫蛋白特异的多克隆免和羊抗体测定每mg来自样品叶粗提物的可溶蛋白中杀昆虫蛋白的量。每个ELISA微量滴定板的加样孔使用50μg总蛋白时，双级三明治ELISA的灵敏度是每mg可溶蛋白1-5ng杀昆虫蛋白。
玉米提取物通过使用手持球珠匀浆器(AGDIA,ELkart IN.)在网状划线的塑料袋中研磨叶组织得到，匀浆器中有抽提缓冲液(50mMNa2CO3PH9.5,100mM NaCl,0.05％ Triton,0.05％ Tween,1mM PMSF和1μM Leupeptim)。蛋白测定使用Bio-Rad(Richmond,CA)蛋白试验进行。4.2亚洲玉米螟分析从玉米植株展开的叶子上切下一个到4个4cm小块。每个叶块放在50×9mm培养皿中湿润的圆滤纸上。在每个叶块上放5只新生亚洲玉米螟幼虫(使每个植株上总数5-20只幼虫)。培养皿放在29.5℃温育。在24,48和72小时后评估叶块饲养损伤和死亡率。实施例5:Ostrinia furnacalis(亚洲玉米螟)田间测试实验将内含事先测试存在并表达了转化基因的转基因幼苗的小泥盆，移载到田野中。非转基因的近交系株系在同一田间载种超过六个星期，以作为对照并供传粉。
当田间植物达到展开叶片长度40cm时，开始用实验室饲养的Ostrinia furnacalis(亚洲玉米螟)幼虫侵袭转基因和非转基因对照植物。使用Davis培养箱，将大约300只与玉米穗轴石细胞团混合的新生幼虫导入每株植物的轮生叶中。以星期为单位，侵袭持续四周以刺激第一代亚洲玉米螟。起始侵袭后两周开始，每周使用分数1-9评估每株植物，持续四周(1=无可见的叶损伤；9=大多数叶有长的伤口，几片叶有折断的中脉，可能由于喂养亚洲玉米螟导致的矮化植株)。对于每株转基因植株和非转基因对照植株，亚洲玉米螟损伤的平均评估分数得到计算。当各植株达到开花期，每周用300幼虫/植株，持续四周，进行第二次侵袭。将各100只在玉米穗轴石细胞团中的新生幼虫导入初生雌穗的叶腋和初生雌穗结的以上和以下一节的叶腋。由此，总数大约2400只幼虫加在每一株植物上。最初二次侵袭后大约50天，收集所有移载的和一些非转基因植株的茎。鉴定第二次侵袭在整个植物中内部穿孔损伤的程度。实施例6测试来自转化原生质体的抽提物抵抗Ostrinia furnacalis(亚洲玉米螟)的活性使用从已瞬间转化DNA以表达玉米最佳化基因的玉米细胞得到的抽提物，进行Western印迹分析。
使用收获的原生质进行数量上的昆虫毒性测试。为在生物分析中所测的每一重复实验制备悬浮液。如果一个重复试验引起明显比对照更高的死亡率，则认为它是阳性的。例如，使用亚洲玉米螟，Ostrinia furnacalis重复测试抽提物针对鳞翅目昆虫的活性。100μl在0.1％Triton X-100中的原生质体悬浮液滴到在50mm×10mm有咬合盖的培养皿中的人工黑色切根虫营养上(Bioserv,Inc.,frenchtown,NJ；F9240)。风干后，每培养皿加入10只新生幼虫。大约4天后记录死亡率。实施例7:Ostrinia furnacalis(亚洲玉米螟)的植物浸泡试验7.1苏云金芽孢杆菌(Bt)晶体苏云金芽孢杆菌(Bt)晶体用22ml双蒸水制备成贮备悬浮液。悬浮液保存于冰箱中。7.2记录规范使用3天大的Ostrinia furnacalis(亚洲玉米螟)幼虫在玉米叶上饲养。幼虫已事先用未处理的叶喂养。120小时后，记录死亡幼虫的数量。观察每个试验中叶上饲养损伤的情况。7.3测试方法使用对亚洲玉米螟易感的玉米(Zea mays)的两个纯合近交系植株(株系A和B)。9-10天大的籽苗在不同浓度的Bt蛋白悬浮液中浸泡，并在喂养实验中使用，其中幼虫放在籽苗的干了的叶子上，每植株5-10只幼虫。籽苗用尼龙网袋盖上，并放在尼龙网笼子中。测试4-5个浓度的4个重复实验，鉴定死亡率。温度保持在21-30℃。7.4结果表2株系A的结果
表3株系B的结果
玉米叶损伤的两种情况清楚地区别出来Bt浸泡的籽苗的叶轻微损伤，同时对照籽苗的叶子严重损伤。
得到下列LC50值株系A:LC50=23.412ppm(从17.834到30.734之间变动)株系B:LC50=12.234 ppm(从9.547到15.676之间变动)实施例8:Ostrinia furnacalis(亚洲玉米螟)的植物浸泡实验(VIP3A)8.1 VIP3A蛋白5mg VIP3A蛋白用50ml双蒸水制备以准备VIP3蛋白的不同浓度100ppm,50ppm,25ppm,12.5ppm,6.25ppm和0ppm(对照)。8.2 Ostrinia furnacalis(亚洲玉米螟)由昆虫学和动物科学部DOA从位于Racha Buri的农场收集蛹以便准备测试用幼虫(L2)8.3记录规范培养5天后，通过计算死亡幼虫的数量收集数据，然后通过概率分析程序分析幼虫死亡的百分率。8.4测试方法使用对亚洲玉米螟易感的玉米(Zea mays)的两个纯合近交系植株(株系B和C)。株系B用来作盆载植物测试(5个不同梯度浓度和对照的4组重复实验)，株系C用于叶浸泡测试(100,50,25ppm和对照的4组重复实验)。10-14天大的籽苗在不同浓度的VIP3A蛋白悬浮液中浸泡，并在喂养实验中使用，其中幼虫放在籽苗的干了的叶子上，每植株5-10幼虫。籽苗用尼龙网袋盖上，并放于尼龙网笼子中。切下的叶子在密封塑料袋中，并置于控制室内，本试验使用4组重复实验。8.4结果表4株系B的结果表4b
表4b
表5株系C的结果表5a
表5b
表5c
表5d
表5e
120小时后得到以下LC50值株系B:LC50=29.558ppm(从21.298到41.022之间变动)株系C:LC50=74.498ppm(从53.644到114.866之间变动)
序列表(1)基本信息(ⅰ)申请人(A)名称Novartis AG(B)街名Schwarzwaldallee 215(C)城市巴塞尔(D)国家瑞士(E)邮政编码(ZIP):4002(F)电话+41 61 69 11 11(G)传真+41 61 696 79 76(H)传真962 991(ⅱ)发明名称控制昆虫虫害的方法(ⅲ)序列数目55(ⅳ)计算机可读形式(A)介质类型软盘(B)计算机IBM PC兼容机(C)操作系统PC-DOS/MS-DOS(D)软件PatentIn Release#1.0，版本#1.30B(2)关于SEQ ID NO:1的信息(ⅰ)序列特征(A)长度6049个核苷酸(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅵ)原始来源
(A)生物蜡状芽孢杆菌(B)菌株AB78(C)特定分离物NRRL B-21058(ⅸ)特征(A)名称/键CDS(B)位置1082..2467(D)其它信息/产品=“VIP2A(a)”(ⅸ)特征(A)名称/键misc feature(B)位置2475..5126(D)其它信息/备注=100kd VIP1A(a)蛋白的编码序列。该编码序列在SEQ ID NO:4中重复并分别翻译。(ⅹⅰ)序列描述SEQ ID NO:1ATCGATACAA TGTTGTTTTA CTTAGACCGG TAGTCTCTGT AATTTGTTTA ATGCTATATT 60CTTTACTTTG ATACATTTTA ATAGCCATTT CAACCTTATC AGTATGTTTT TGTGGTCTTC 120CTCCTTTTTT TCCACGAGCT CTAGCTGCGT TTAATCCTGT TTTGGTACGT TCGCTAATAA 180TATCTCTTTC TAATTCTGCA ATACTTGCCA TCATTCGAAA CAAGAATTTC CCCATAGCAT 240TAGAGGTATC AATGTTGTCA TGAATAGAAA TAAAATCTAC ACCTAGCTCT TTGAATTTTT 300CACTTAACTC AATTAGGTGT TTTGTAGAGC GAGAAATTCG ATCAAGTTTG TAAACAACTA 360TCTTATCGCC TTTACGTAAT ACTTTTAGCA ACTCTTCGAG TTGAGGGCGC TCTTTTTTTA 420TTCCTGTTAT TTTCTCCTGA TATAGCCTTT CTACACCATA TTGTTGCAAA GCATCTATTT 480GCATATCGAG ATTTTGTTCT TCTGTGCTGA CACGAGCATA ACCAAAAATC AAATTGGTTT 540CACTTCCTAT CTAAATATAT CTATTAAAAT AGCACCAAAA ACCTTATTAA ATTAAAATAA 600GGAACTTTGT TTTTGGATAT GGATTTTGGT ACTCAATATG GATGAGTTTT TAACGCTTTT 660GTTAAAAAAC AAACAAGTGC CATAAACGGT CGTTTTTGGG ATGACATAAT AAATAATCTG 720TTTGATTAAC CTAACCTTGT ATCCTTACAG CCCAGTTTTA TTTGTACTTC AACTGACTGA 780ATATGAAAAC AACATGAAGG TTTCATAAAA TTTATATATT TTCCATAACG GATGCTCTAT 840CTTTAGGTTA TAGTTAAATT ATAAGAAAAA AACAAACGGA GGGAGTGAAA AAAAGCATCT 900TCTCTATAAT TTTACAGGCT CTTTAATAAG AAGGGGGGAG ATTAGATAAT AAATATGAAT 960ATCTATCTAT AATTGTTTGC TTCTACAATA ACTTATCTAA CTTTCATATA CAACAACAAA1020ACAGACTAAA TCCAGATTGT ATATTCATTT TCAGTTGTTC CTTTATAAAA TAATTTCATA1080A ATG AAA AGA ATG GAG GGA AAG TTG TTT ATG GTG TCA AAA AAA TTA1126Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu1 5 10 15CAA GTA GTT ACT AAA ACT GTA TTG CTT AGT ACA GTT TTC TCT ATA TCT 1174Gln Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser20 25 30TTA TTA AAT AAT GAA GTG ATA AAA GCT GAA CAA TTA AAT ATA AAT TCT 1222Leu Leu Asn Asn Glu Val Ile Lys Ala Glu Gln Leu Asn Ile Asn Ser35 40 45CAA AGT AAA TAT ACT AAC TTG CAA AAT CTA AAA ATC ACT GAC AAG GTA 1270Gln Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Thr Asp Lys Val50 55 60GAG GAT TTT AAA GAA GAT AAG GAA AAA GCG AAA GAA TGG GGG AAA GAA 1318Glu Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu65 70 75AAA GAA AAA GAG TGG AAA CTA ACT GCT ACT GAA AAA GGA AAA ATG AAT 1366Lys Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn80 85 90 95AAT TTT TTA GAT AAT AAA AAT GAT ATA AAG ACA AAT TAT AAA GAA ATT 1414Asn Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile100 105 110ACT TTT TCT ATG GCA GGC TCA TTT GAA GAT GAA ATA AAA GAT TTA AAA 1462Thr Phe Ser Met Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys115 120 125GAA ATT GAT AAG ATG TTT GAT AAA ACC AAT CTA TCA AAT TCT ATT ATC 1510Glu Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile130 135 140ACC TAT AAA AAT GTG GAA CCG ACA ACA ATT GGA TTT AAT AAA TCT TTA 1558Thr Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu145150 155ACA GAA GGT AAT ACG ATT AAT TCT GAT GCA ATG GCA CAG TTT AAA GAA 1606Thr Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu160 165 170 175CAA TTT TTA GAT AGG GAT ATT AAG TTT GAT AGT TAT CTA GAT ACG CAT 1654Gln Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His180 185 190TTA ACT GCT CAA CAA GTT TCC AGT AAA GAA AGA GTT ATT TTG AAG GTT 1702Leu Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val195 200 205ACG GTT CCG AGT GGG AAA GGT TCT ACT ACT CCA ACA AAA GCA GGT GTC 1750Thr Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val210 215 220ATT TTA AAT AAT AGT GAA TAC AAA ATG CTC ATT GAT AAT GGG TAT ATG 1798Ile Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met225 230 235GTC CAT GTA GAT AAG GTA TCA AAA GTG GTG AAA AAA GGG GTG GAG TGC 1846Val His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys240 245 250 255TTA CAA ATT GAA GGG ACT TTA AAA AAG AGT CTT GAC TTT AAA AAT GAT 1894Leu Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp260 265 270ATA AAT GCT GAA GCG CAT AGC TGG GGT ATG AAG AAT TAT GAA GAG TGG 1942Ile Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp275 280 285GCT AAA GAT TTA ACC GAT TCG CAA AGG GAA GCT TTA GAT GGG TAT GCT 1990Ala Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala290 295 300AGG CAA GAT TAT AAA GAA ATC AAT AAT TAT TTA AGA AAT CAA GGC GGA 2038Arg Gln Asp Tyr Lys Glu Ile Asn Ash Tyr Leu Arg Asn Gln Gly Gly305 310 315AGT GGA AAT GAA AAA CTA GAT GCT CAA ATA AAA AAT ATT TCT GAT GCT 2086Ser Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala320 325 330 335TTA GGG AAG AAA CCA ATA CCG GAA AAT ATT ACT GTG TAT AGA TGG TGT 2134Leu Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys340 345 350GGC ATG CCG GAA TTT GGT TAT CAA ATT AGT GAT CCG TTA CCT TCT TTA 2182Gly Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu355 360 365AAA GAT TTT GAA GAA CAA TTT TTA AAT ACA ATC AAA GAA GAC AAA GGA 2230Lys Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly370 375 380TAT ATG AGT ACA AGC TTA TCG AGT GAA CGT CTT GCA GCT TTT GGA TCT 2278Tyr Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser385 390 395AGA AAA ATT ATA TTA CGA TTA CAA GTT CCG AAA GGA AGT ACG GGT GCG 2326Arg Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala400 405 410 415TAT TTA AGT GCC ATT GGT GGA TTT GCA AGT GAA AAA GAG ATC CTA CTT 2374Tyr Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu420 425 430GAT AAA GAT AGT AAA TAT CAT ATT GAT AAA GTA ACA GAG GTA ATT ATT 2422Asp Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile435 440 445AAA GGT GTT AAG CGA TAT GTA GTG GAT GCA ACA TTA TTA ACA AAT 2467Lys Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn450 455 460TAAGGAGATG AAAAATATGA AGAAAAAGTT AGCAAGTGTT GTAACGTGTA CGTTATTAGC2527TCCTATGTTT TTGAATGGAA ATGTGAATGC TGTTTACGCA GACAGCAAAA CAAATCAAAT2587TTCTACAACA CAGAAAAATC AACAGAAAGA GATGGACCGA AAAGGATTAC TTGGGTATTA2647TTTCAAAGGA AAAGATTTTA GTAATCTTAC TATGTTTGCA CCGACACGTG ATAGTACTCT2707TATTTATGAT CAACAAACAG CAAATAAACT ATTAGATAAA AAACAACAAG AATATCAGTC2767TATTCGTTGG ATTGGTTTGA TTCAGAGTAA AGAAACGGGA GATTTCACAT TTAACTTATC2827TGAGGATGAA CAGGCAATTA TAGAAATCAA TGGGAAAATT ATTTCTAATA AAGGGAAAGA2887AAAGCAAGTT GTCCATTTAG AAAAAGGAAA ATTAGTTCCA ATCAAAATAG AGTATCAATC2947AGATACAAAA TTTAATATTG ACAGTAAAAC ATTTAAAGAA CTTAAATTAT TTAAAATAGA3007TAGTCAAAAC CAACCCCAGC AAGTCCAGCA AGATGAACTG AGAAATCCTG AATTTAACAA3067GAAAGAATCA CAGGAATTCT TAGCGAAACC ATCGAAAATA AATCTTTTCA CTCAAAAAAT3127GAAAAGGGAA ATTGATGAAG ACACGGATAC GGATGGGGAC TCTATTCCTG ACCTTTGGGA3187AGAAAATGGG TATACGATTC ACAATAGAAT CGCTGTAAAG TGGGACGATT CTCTAGCAAG3247TAAAGGGTAT ACGAAATTTG TTTCAAATCC ACTAGAAAGT CACACAGTTG GTGATCCTTA3307TACAGATTAT GAAAAGGCAG CAAGAGATCT AGATTTGTCA AATGCAAAGG AAACGTTTAA3367CCCATTGGTA GCTGCTTTTC CAAGTGTGAA TGTTAGTATG GAAAAGGTGA TATTATCACC3427AAATGAAAAT TTATCCAATA GTGTAGAGTC TCATTCATCC ACGAATTGGT CTTATACAAA3487TACAGAAGGT GCTTCTGTTG AAGCGGGGAT TGGACCAAAA GGTATTTCGT TCGGAGTTAG3547CGTAAACTAT CAACACTCTG AAACAGTTGC ACAAGAATGG GGAACATCTA CAGGAAATAC3607TTCGCAATTC AATACGGCTT CAGCGGGATA TTTAAATGCA AATGTTCGAT ATAACAATGT3667AGGAACTGGT GCCATCTACG ATGTAAAACC TACAACAAGT TTTGTATTAA ATAACGATAC3727TATCGCAACT ATTACGGCGA AATCTAATTC TACAGCCTTA AATATATCTC CTGGAGAAAG3787TTACCCGAAA AAAGGACAAA ATGGAATCGC AATAACATCA ATGGATGATT TTAATTCCCA3847TCCGATTACA TTAAATAAAA AACAAGTAGA TAATCTGCTA AATAATAAAC CTATGATGTT3907GGAAACAAAC CAAACAGATG GTGTTTATAA GATAAAAGAT ACACATGGAA ATATAGTAAC3967TGGCGGAGAA TGGAATGGTG TCATACAACA AATCAAGGCT AAAACAGCGT CTATTATTGT4027GGATGATGGG GAACGTGTAG CAGAAAAACG TGTAGCGGCA AAAGATTATG AAAATCCAGA4087AGATAAAACA CCGTCTTTAA CTTTAAAAGA TGCCCTGAAG CTTTCATATC CAGATGAAAT4147AAAAGAAATA GAGGGATTAT TATATTATAA AAACAAACCG ATATACGAAT CGAGCGTTAT4207GACTTACTTA GATGAAAATA CAGCAAAAGA AGTGACCAAA CAATTAAATG ATACCACTGG4267GAAATTTAAA GATGTAAGTC ATTTATATGA TGTAAAACTG ACTCCAAAAA TGAATGTTAC4327AATCAAATTG TCTATACTTT ATGATAATGC TGAGTCTAAT GATAACTCAA TTGGTAAATG4387GACAAACACA AATATTGTTT CAGGTGGAAA TAACGGAAAA AAACAATATT CTTCTAATAA4447TCCGGATGCT AATTTGACAT TAAATACAGA TGCTCAAGAA AAATTAAATA AAAATCGTGA4507CTATTATATA AGTTTATATA TGAAGTCAGA AAAAAACACA CAATGTGAGA TTACTATAGA4567TGGGGAGATT TATCCGATCA CTACAAAAAC AGTGAATGTG AATAAAGACA ATTACAAAAG4627ATTAGATATT ATAGCTCATA ATATAAAAAG TAATCCAATT TCTTCACTTC ATATTAAAAC4687GAATGATGAA ATAACTTTAT TTTGGGATGA TATTTCTATA ACAGATGTAG CATCAATAAA4747ACCGGAAAAT TTAACAGATT CAGAAATTAA ACAGATTTAT AGTAGGTATG GTATTAAGTT4807AGAAGATGGA ATCCTTATTG ATAAAAAAGG TGGGATTCAT TATGGTGAAT TTATTAATGA4867AGCTAGTTTT AATATTGAAC CATTGCAAAA TTATGTGACC AAATATGAAG TTACTTATAG4927TAGTGAGTTA GGACCAAACG TGAGTGACAC ACTTGAAAGT GATAAAATTT ACAAGGATGG4987GACAATTAAA TTTGATTTTA CCAAATATAG TAAAAATGAA CAAGGATTAT TTTATGACAG5047TGGATTAAAT TGGGACTTTA AAATTAATGC TATTACTTAT GATGGTAAAG AGATGAATGT5107TTTTCATAGA TATAATAAAT AGTTATTATA TCTATGAAGC TGGTGCTAAA GATAGTGTAA5167AAGTTAATAT ACTGTAGGAT TGTAATAAAA GTAATGGAAT TGATATCGTA CTTTGGAGTG5227GGGGATACTT TGTAAATAGT TCTATCAGAA ACATTAGACT AAGAAAAGTT ACTACCCCCA5287CTTGAAAATG AAGATTCAAC TGATTACAAA CAACCTGTTA AATATTATAA GGTTTTAACA5347AAATATTAAA CTCTTTATGT TAATACTGTA ATATAAAGAG TTTAATTGTA TTCAAATGAA5407GCTTTCCCAC AAA TTAGAC TGATTATCTA ATGAAATAAT CAGTCTAATT TTGTAGAACA5467GGTCTGGTAT TATTGTACGT GGTCACTAAA AGATATCTAA TATTATTGGG CAAGGCGTTC5527CATGATTGAA TCCTCGAATG TCTTGCCCTT TTCATTTATT TAAGAAGGAT TGTGGAGAAA5587TTATGGTTTA GATAATGAAG AAAGACTTCA CTTCTAATTT TTGATGTTAA ATAAATCAAA5647ATTTGGCGAT TCACATTGTT TAATCCACTG ATAAAACATA CTGGAGTGTT CTTAAAAAAT5707CAGCTTTTTT CTTTATAAAA TTTTGCTTAG CGTACGAAAT TCGTGTTTTG TTGGTGGGAC5767CCCATGCCCA TCAACTTAAG AGTAAATTAG TAATGAACTT TCGTTCATCT GGATTAAAAT5827AACCTCAAAT TAGGACATGT TTTTAAAAAT AAGCAGACCA AATAAGCCTA GAATAGGTAT5887CATTTTTAAA AATTATGCTG CTTTCTTTTG TTTTCCAAAT CCATTATACT CATAAGCAAC5947ACCCATAATG TCAAAGACTG TTTTTGTCTC ATATCGATAA GCTTGATATC GAATTCCTGC6007AGCCCGGGGG ATCCACTAGT TCTAGAGCGG CCGCCACCGC GG 6049(2)关于SEQ ID NO:2的信息(ⅰ)序列特征(A)长度462个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:2:Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln1 5 10 15Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu20 25 30Leu Asn Asn Glu Val Ile Lys Ala Glu Gln Leu Asn Ile Asn Ser Gln35 40 45Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu50 55 60Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys65 70 75 80Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn85 90 95Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr100 105 110Phe Ser Met Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu115 120 125Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr130 135 140Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr145 150 155 160Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln165 170 175Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu180 185 190Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr195 200 205Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile210 215 220Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val225 230 235 240His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu245 250 255Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile260 265 270Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala275 280 2855ys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg290 295 300Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser305 310 315 320Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu325 330 335Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly340 345 350Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys355 360 365Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr370 375 380Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg385 390 395 400Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr405 410 415Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp420 425 430Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys435 440 445Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn450 455 460(2)关于SEQ ID NO:3的信息(ⅰ)序列特征(A)长度20个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型肽(ⅸ)特征(A)名称/键肽(B)位置1..20(D)其它信息/备注=用于导向液泡的信号肽(ⅹⅰ)序列描述SEQ ID NO:3:Ser Ser Ser Ser Phe Ala Asp Ser Asn Pro Ile Arg Val Thr Asp Arg1 5 10 15Ala Ala Ser Thr20(2)关于SEQ ID NO:4的信息(ⅰ)序列特征(A)长度2655个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅳ)反义否(ⅵ)原始来源(A)生物蜡状芽孢杆菌(B)菌株AB78(C)特定分离物NRRL B-21058(ⅸ)特征(A)名称/键CDS(B)位置1..2652(D)其它信息/产品=“100kDa VIP1A(a)蛋白”，/备注=“该序列与SEQ ID NO:1的从2475到5126位的部分核苷酸相同。”(ⅹⅰ)序列描述SEQ ID NO:4:ATG AAA AAT ATG AAG AAA AAG TTA GCA AGT GTT GTA ACG TGT ACG TTA48Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Thr Leu465 470 475TTA GCT CCT ATG TTT TTG AAT GGA AAT GTG AAT GCT GTT TAC GCA GAC96Leu Ala Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Tyr Ala Asp480 485 490AGC AAA ACA AAT CAA ATT TCT ACA ACA CAG AAA AAT CAA CAG AAA GAG 144Ser Lys Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln Lys Glu495 500 505 510ATG GAC CGA AAA GGA TTA CTT GGG TAT TAT TTC AAA GGA AAA GAT TTT 192Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe515 520 525AGT AAT CTT ACT ATG TTT GCA CCG ACA CGT GAT AGT ACT CTT ATT TAT 240Ser Asn Leu Thr Met Phe Ala Pro Thr Arg Asp Ser Thr Leu Ile Tyr530 535 540GAT CAA CAA ACA GCA AAT AAA CTA TTA GAT AAA AAA CAA CAA GAA TAT 288Asp Gln Gln Thr Ala Asn Lys Leu Leu Asp Lys Lys Gln GLn Glu Tyr545 550 555CAG TCT ATT CGT TGG ATT GGT TTG ATT CAG AGT AAA GAA ACG GGA GAT 336Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln Ser Lys Glu Thr Gly Asp560 565 570TTC ACA TTT AAC TTA TCT GAG GAT GAA CAG GCA ATT ATA GAA ATC AAT 384Phe Thr Phe Asn Leu Ser Glu Asp Glu Gln Ala Ile Ile Glu Ile Asn575 580 585 590GGG AAA ATT ATT TCT AAT AAA GGG AAA GAA AAG CAA GTT GTC CAT TTA 432Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val His Leu595 600 605GAA AAA GGA AAA TTA GTT CCA ATC AAA ATA GAG TAT CAA TCA GAT ACA 480Glu Lys Gly Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser Asp Thr610 615 620AAA TTT AAT ATT GAC AGT AAA ACA TTT AAA GAA CTT AAA TTA TTT AAA 528Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu Phe Lys625 630 635ATA GAT AGT CAA AAC CAA CCC CAG CAA GTC CAG CAA GAT GAA CTG AGA 576Ile Asp Ser Gln Asn Gln Pro Gln Gln Val Gln Gln Asp Glu Leu Arg640 645 650AAT CCT GAA TTT AAC AAG AAA GAA TCA CAG GAA TTC TTA GCG AAA CCA 624Asn Pro Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Pro655 660 665 670TCG AAA ATA AAT CTT TTC ACT CAA AAA ATG AAA AGG GAA ATT GAT GAA 672Ser Lys Ile Asn Leu Phe Thr Gln Lys Met Lys Arg Glu Ile Asp Glu675 680 685GAC ACG GAT ACG GAT GGG GAC TCT ATT CCT GAC CTT TGG GAA GAA AAT720Asp Thr Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn690 695 700GGG TAT ACG ATT CAA AAT AGA ATC GCT GTA AAG TGG GAC GAT TCT CTA768Gly Tyr Thr Ile Gln Asn Arg Ile Ala Val Lys Trp Asp Asp Ser Leu705 710 715GCA AGT AAA GGG TAT ACG AAA TTT GTT TCA AAT CCA CTA GAA AGT CAC816Ala Ser Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Glu Ser His720 725 730ACA GTT GGT GAT CCT TAT ACA GAT TAT GAA AAG GCA GCA AGA GAT CTA864Thr Val Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg Asp Leu735 740 745 750GAT TTG TCA AAT GCA AAG GAA ACG TTT AAC CCA TTG GTA GCT GCT TTT912Asp Leu Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe755 760 765CCA AGT GTG AAT GTT AGT ATG GAA AAG GTG ATA TTA TCA CCA AAT GAA960Pro Ser Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu770 775 780AAT TrA TCC AAT AGT GTA GAG TCT CAT TCA TCC ACG AAT TGG TCT TAT 1008Asn Leu Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr785790 795ACA AAT ACA GAA GGT GCT TCT GTT GAA GCG GGG ATT GGA CCA AAA GGT 1056Thr Asn Thr Glu Gly Ala Ser Val Glu Ala Gly Ile Gly Pro Lys Gly800 805 810ATT TCG TTC GGA GTT AGC GTA AAC TAT CAA CAC TCT GAA ACA GTT GCA 1104Ile Ser Phe Gly Val Ser Val Asn Tyr Gln His Ser Glu Thr Val Ala815 820 825 830CAA GAA TGG GGA ACA TCT ACA GGA AAT ACT TCG CAA TTC AAT ACG GCT 1152Gln Glu Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala835 840 845TCA GCG GGA TAT TTA AAT GCA AAT GTT CGA TAT AAC AAT GTA GGA ACT 1200Ser Ala Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr850 855 860GGT GCC ATC TAC GAT GTA AAA CCT ACA ACA AGT TTT GTA TTA AAT AAC 1248Gly Ala Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn865 870 875GAT ACT ATC GCA ACT ATT ACG GCG AAA TCT AAT TCT ACA GCC TTA AAT 1296Asp Thr Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Asn880 885 890ATA TCT CCT GGA GAA AGT TAC CCG AAA AAA GGA CAA AAT GGA ATC GCA 1344Ile Ser Pro Gly Glu Ser Tyr Pro Lys Lys Gly Gln Asn Gly Ile Ala895 900 905 910ATA ACA TCA ATG GAT GAT TTT AAT TCC CAT CCG ATP ACA TTA AAT AAA 1392Ile Thr Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys915 920 925AAA CAA GTA GAT AAT CTG CTA AAT AAT AAA CCT ATG ATG TTG GAA ACA 1440Lys Gln Val Asp Asn Leu Leu Asn Asn Lys Pro Met Met Leu Glu Thr930 935 940AAC CAA ACA GAT GGT GTT TAT AAG ATA AAA GAT ACA CAT GGA AAT ATA 1488Asn Gln Thr Asp Gly Val Tyr Lys Ile Lys Asp Thr His Gly Asn Ile945 950 955GTA ACT GGC GGA GAA TGG AAT GGT GTC ATA CAA CAA ATC AAG GCT AAA 1536Val Thr Gly Gly Glu Trp Asn Gly Val Ile Gln Gln Ile Lys Ala Lys960 965 970ACA GCG TCT ATT ATT GTG GAT GAT GGG GAA CGT GTA GCA GAA AAA CGT 1584Thr Ala Ser Ile Ile Val Asp Asp Gly Glu Arg Val Ala Glu Lys Arg975 980 985 990GTA GCG GCA AAA GAT TAT GAA AAT CCA GAA GAT AAA ACA CCG TCT TTA 1632Val Ala Ala Lys Asp Tyr Glu Asn Pro Glu Asp Lys Thr Pro Ser Leu995 10001005ACT TTA AAA GAT GCC CTG AAG CTT TCA TAT CCA GAT GAA ATA AAA GAA 1680Thr Leu Lys Asp Ala Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu101010151020ATA GAG GGA TTA TTA TAT TAT AAA AAC AAA CCG ATA TAC GAA TCG AGC 1728Ile Glu Gly Leu Leu Tyr Tyr Lys Asn Lys Pro Ile Tyr Glu Ser Ser102510301035GTT ATG ACT TAC TTA GAT GAA AAT ACA GCA AAA GAA GTG ACC AAA CAA 1776Val Met Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Thr Lys Gln104010451050TTA AAT GAT ACC ACT GGG AAA TTT AAA GAT GTA AGT CAT TTA TAT GAT 1824Leu Asn Asp Thr Thr Gly Lys Phe Lys Asp Val Ser His Leu Tyr Asp1055106010651070GTA AAA CTG ACT CCA AAA ATG AAT GTT ACA ATC AAA TTG TCT ATA CTT 1872Val Lys Leu Thr Pro Lys Met Asn Val Thr Ile Lys Leu Ser Ile Leu107510801085TAT GAT AAT GCT GAG TCT AAT GAT AAC TCA ATT GGT AAA TGG ACA AAC 1920Tyr Asp Asn Ala Glu Ser Asn Asp Asn Ser Ile Gly Lys Trp Thr Asn109010951100ACA AAT ATT GTT TCA GGT GGA AAT AAC GGA AAA AAA CAA TAT TCT TCT 1968Thr Asn Ile Val Ser Gly Gly Asn Asn Gly Lys Lys Gln Tyr Ser Ser110511101115AAT AAT CCG GAT GCT AAT TTG ACA TTA AAT ACA GAT GCT CAA GAA AAA 2016Asn Asn Pro Asp Ala Asn Leu Thr Leu Asn Thr Asp Ala Gln Glu Lys112011251130TTA AAT AAA AAT CGT GAC TAT TAT ATA AGT TTA TAT ATG AAG TCA GAA 2064Leu Asn Lys Asn Arg Asp Tyr Tyr Ile Ser Leu Tyr Met Lys Ser Glu1135114011451150AAA AAC ACA CAA TGT GAG ATT ACT ATA GAT GGG GAG ATT TAT CCG ATC 2112Lys Asn Thr Gln Cys Glu Ile Thr Ile Asp Gly Glu Ile Tyr Pro Ile115511601165ACT ACA AAA ACA GTG AAT GTG AAT AAA GAC AAT TAC AAA AGA TTA GAT 2160Thr Thr Lys Thr Val Asn Val Asn Lys Asp Asn Tyr Lys Arg Leu Asp117011751180ATT ATA GCT CAT AAT ATA AAA AGT AAT CCA ATT TCT TCA CTT CAT ATT 2208Ile Ile Ala His Asn Ile Lys Ser Asn Pro Ile Ser Ser Leu His Ile118511901195AAA ACG AAT GAT GAA ATA ACT TTA TTT TGG GAT GAT ATT TCT ATA ACA 2256Lys Thr Asn Asp Glu Ile Thr Leu Phe Trp Asp Asp Ile Ser Ile Thr1200 12051210GAT GTA GCA TCA ATA AAA CCG GAA AAT TTA ACA GAT TCA GAA ATT AAA 2304Asp Val Ala Ser Ile Lys Pro Glu Asn Leu Thr Asp Ser Glu Ile Lys1215122012251230CAG ATT TAT AGT AGG TAT GGT ATT AAG TTA GAA GAT GGA ATC CTT ATT 2352Gln Ile Tyr Ser Arg Tyr Gly Ile Lys Leu Glu Asp Gly Ile Leu Ile123512401245GAT AAA AAA GGT GGG ATT CAT TAT GGT GAA TTT ATT AAT GAA GCT AGT 2400Asp Lys Lys Gly Gly Ile His Tyr Gly Glu Phe Ile Asn Glu Ala Ser125012551260TTT AAT ATT GAA CCA TTG CAA AAT TAT GTG ACC AAA TAT GAA GTT ACT 2448Phe Asn Ile Glu Pro Leu Gln Asn Tyr Val Thr Lys Tyr Glu Val Thr126512701275TAT AGT AGT GAG TTA GGA CCA AAC GTG AGT GAC ACA CTT GAA AGT GAT 2496Tyr Ser Ser Glu Leu Gly Pro Asn Val Ser Aso Thr Leu Glu Ser Asp128012851290AAA ATT TAC AAG GAT GGG ACA ATT AAA TTT GAT TTT ACC AAA TAT AGT 2544Lys Ile Tyr Lys Asp Gly Thr Ile Lys Phe Asp Phe Thr Lys Tys Ser1295130013051310AAA AAT GAA CAA GGA TTA TTT TAT GAC AGT GGA TTA AAT TGG GAC TTT 2592Lys Asn Glu Gln Gly Leu Phe Tyr Asp Ser Gly Leu Asn Trp Asp Phe131513201325AAA ATT AAT GCT ATT ACT TAT GAT GGT AAA GAG ATG AAT GTT TTT CAT 2640Lys Ile Asn Ala Ile Thr Tyr Asp Gly Lys Glu Met Asn Val Phe His133013351340AGA TAT AAT AAA TAG 2655Arg Tyr Asn Lys1345(2)关于SEQ ID NO:5的信息(ⅰ)序列特征(A)长度884个氨基酸
(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:5:Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Thr Leu1 5 10 15Leu Ala Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Tyr Ala Asp20 25 30Ser Lys Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln Lys Glu35 40 45Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe50 55 60Ser Asn Leu Thr Met Phe Ala Pro Thr Arg Asp Ser Thr Leu Ile Tyr65 70 75 80Asp Gln Gln Thr Ala Asn Lys Leu Leu Asp Lys Lys Gln Gln Glu Tyr85 90 95Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln Ser Lys Glu Thr Gly Asp100 105 110Phe Thr Phe Asn Leu Ser Glu Asp Glu Gln Ala Ile Ile Glu Ile Asn115 120 125Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val His Leu130 135 140Glu Lys Gly Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser Asp Thr145 150 155 160Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu Phe Lys165 170 175Ile Asp Ser Gln Asn Gln Pro Gln Gln Val Gln Gln Asp Glu Leu Arg180 185 190Asn Pro Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Pro195 200 205Ser Lys Ile Asn Leu Phe Thr Gln Lys Met Lys Arg Glu Ile Asp Glu210 215 220Asp Thr Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn225 230 235 240Gly Tyr Thr Ile Gln Asn Arg Ile Ala Val Lys Trp Asp Asp Ser Leu245 250 255Ala Ser Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Glu Ser His260 265 270Thr Val Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg Asp Leu275 280 285Asp Leu Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe290 295 300Pro Ser Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu305 310 315 320Asn Leu Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr325 330 335Thr Asn Thr Glu Gly Ala Ser Val Glu Ala Gly Ile Gly Pro Lys Gly340 345 350Ile Ser Phe Gly Val Ser Val Asn Tyr Gln His Ser Glu Thr Val Ala355 360 365Gln Glu Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala370 375 380Ser Ala Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr385 390 395 400Gly Ala Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn405 410 415Asp Thr Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Asn420 425 430Ile Ser Pro Gly Glu Ser Tyr Pro Lys Lys Gly Gln Asn Gly Ile Ala435 440 445Ile Thr Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys450 455 460Lys Gln Val Asp Asn Leu Leu Asn Asn Lys Pro Met Met Leu Glu Thr465 470 475 480Asn Gln Thr Asp Gly Val Tyr Lys Ile Lys Asp Thr His Gly Asn Ile485 490 495Val Thr Gly Gly Glu Trp Asn Gly Val Ile Gln Gln Ile Lys Ala Lys500 505 510Thr Ala Ser Ile Ile Val Asp Asp Gly Glu Arg Val Ala Glu Lys Arg515 520 525Val Ala Ala Lys Asp Tyr Glu Asn Pro Glu Asp Lys Thr Pro Ser Leu530 535 540Thr Leu Lys Asp Ala Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu545 550 555560Ile Glu Gly Leu Leu Tyr Tyr Lys Asn Lys Pro Ile Tyr Glu Ser Ser565 570 575Val Met Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Thr Lys Gln580 585 590Leu Asn Asp Thr Thr Gly Lys Phe Lys Asp Val Ser His Leu Tyr Asp595 600 605Val Lys Leu Thr Pro Lys Met Asn Val Thr Ile Lys Leu Ser Ile Leu610 615 620Tyr Asp Asn Ala Glu Ser Asn Asp Asn Ser Ile Gly Lys Trp Thr Asn625 630 635 640Thr Asn Ile Val Ser Gly Gly Asn Asn Gly Lys Lys Gln Tyr Ser Ser645 650 655Asn Asn Pro Asp Ala Asn Leu Thr Leu Asn Thr Asp Ala Gln Glu Lys660 665 670Leu Asn Lys Asn Arg Asp Tyr Tyr Ile Ser Leu Tyr Met Lys Ser Glu675 680 685Lys Asn Thr Gln Cys Glu Ile Thr Ile Asp Gly Glu Ile Tyr Pro Ile690 695 700Thr Thr Lys Thr Val Asn Val Asn Lys Asp Asn Tyr Lys Arg Leu Asp705 710 715 720Ile Ile Ala His Asn Ile Lys Ser Asn Pro Ile Ser Ser Leu His Ile725 730 735Lys Thr Asn Asp Glu Ile Thr Leu Phe Trp Asp Asp Ile Ser Ile Thr740 745 750Asp Val Ala Ser Ile Lys Pro Glu Asn Leu Thr Asp Ser Glu Ile Lys755 760 765Gln Ile Tyr Ser Arg Tyr Gly Ile Lys Leu Glu Asp Gly Ile Leu Ile770 775 780Asp Lys Lys Gly Gly Ile His Tyr Gly Glu Phe Ile Asn Glu Ala Ser785 790 795 800Phe Asn Ile Glu Pro Leu Gln Asn Tyr Val Thr Lys Tyr Glu Val Thr805 810 815Tyr Ser Ser Glu Leu Gly Pro Asn Val Ser Asp Thr Leu Glu Ser Asp820 825 830Lys Ile Tyr Lys Asp Gly Thr Ile Lys Phe Asp Phe Thr Lys Tyr Ser
835 840 845Lys Asn Glu Gln Gly Leu Phe Tyr Asp Ser Gly Leu Asn Trp Asp Phe850 855 860Lys Ile Asn Ala Ile Thr Tyr Asp Gly Lys Glu Met Asn Val Phe His865 870 875 880Arg Tyr Asn Lys(2)关于SEQ ID NO:6的信息(ⅰ)序列特征(A)长度2004个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅳ)反义否(ⅵ)原始来源(A)生物蜡状芽孢杆菌(B)菌株AB78(C)特定分离物NRRL B-21058(ⅸ)特征(A)名称/键CDS(B)位置1..2001(D)其它信息/产品=“80kDa VIP1A(a)蛋白”，/备注=“该序列与SEQ ID NO:1的从3126到5126位的核苷酸相同。”(ⅹⅰ)序列描述SEQ ID NO:6:ATG AAA AGG GAA ATT GAT GAA GAC ACG GAT ACG GAT GGG GAC TCT ATT 48Met Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser Ile885 890 895 900CCT GAC CTT TGG GAA GAA AAT GGG TAT ACG ATT CAA AAT AGA ATC GCT 96Pro Asp Leu Trp Glu Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile Ala905 910 915GTA AAG TGG GAC GAT TCT CTA GCA AGT AAA GGG TAT ACG AAA TTT GTT144Val Lys Trp Asp Asp Ser Leu Ala Ser Lys Gly Tyr Thr Lys Phe Val920 925 930TCA AAT CCA CTA GAA AGT CAC ACA GTT GGT GAT CCT TAT ACA GAT TAT192Ser Asn Pro Leu Glu Ser His Thr Val Gly Asp Pro Tyr Thr Asp Tyr935 940 945GAA AAG GCA GCA AGA GAT CTA GAT TTG TCA AAT GCA AAG GAA ACG TTT240Glu Lys Ala Ala Arg Asp Leu Asp Leu Ser Asn Ala Lys Glu Thr Phe950 955 960AAC CCA TTG GTA GCT GCT TTT CCA AGT GTG AAT GTT AGT ATG GAA AAG288Asn Pro Leu Val Ala Ala Phe Pro Ser Val Asn Val Ser Met Glu Lys965 970 975 980GTG ATA TTA TCA CCA AAT GAA AAT TTA TCC AAT AGT GTA GAG TCT CAT336Val Ile Leu Ser Pro Asn Glu Asn Leu Ser Asn Ser Val Glu Ser His985 990 995TCA TCC ACG AAT TGG TCT TAT ACA AAT ACA GAA GGT GCT TCT GTT GAA384Ser Ser Thr Asn Trp Ser Tyr Thr Asn Thr Glu Gly Ala Ser Val Glu100010051010GCG GGG ATT GGA CCA AAA GGT ATT TCG TTC GGA GTT AGC GTA AAC TAT432Ala Gly Ile Gly Pro Lys Gly Ile Ser Phe Gly Val Ser Val Asn Tyr101510201025CAA CAC TCT GAA ACA GTT GCA CAA GAA TGG GGA ACA TCT ACA GGA AAT480Gln His Ser Glu Thr Val Ala Gln Glu Trp Gly Thr Ser Thr Gly Asn103010351040ACT TCG CAA TTC AAT ACG GCT TCA GCG GGA TAT TTA AAT GCA AAT GTT528Thr Ser Gln Phe Asn Thr Ala Ser Ala Gly Tyr Leu Asn Ala Asn Val1045105010551060CGA TAT AAC AAT GTA GGA ACT GGT GCC ATC TAC GAT GTA AAA CCT ACA576Arg Tyr Asn Asn Val Gly Thr Gly Ala Ile Tyr Asp Val Lys Pro Thr106510701075ACA AGT TTT GTA TTA AAT AAC GAT ACT ATC GCA ACT ATT ACG GCG AAA624Thr Ser Phe Val Leu Asn Asn Asp Thr Ile Ala Thr Ile Thr Ala Lys108010851090TCT AAT TCT ACA GCC TTA AAT ATA TCT CCT GGA GAA AAT TAC CCG AAA672Ser Asn Ser Thr Ala Leu Asn Ile Ser Pro Gly Glu Ser Tyr Pro Lys109511001105AAA GGA CAA AAT GGA ATC GCA ATA ACA TCA ATG GAT GAT TTT AAT TCC720Lys Gly Gln Asn Gly Ile Ala Ile Thr Ser Met Asp Asp Phe Asn Ser111011151120CAT CCG ATT ACA TTA AAT AAA AAA CAA GTA GAT AAT CTG CTA AAT AAT768His Pro Ile Thr Leu Asn Lys Lys Gln Val Asp Asn Leu Leu Asn Asn1125113011351140AAA CCT ATG ATG TTG GAA ACA AAC CAA ACA GAT GGT GTT TAT AAG ATA816Lys Pro Met Met Leu Glu Thr Asn Gln Thr Asp Gly Val Tyr Lys Ile114511501155AAA GAT ACA CAT GGA AAT ATA GTA ACT GGC GGA GAA TGG AAT GGT GTC864Lys Asp Thr His Gly Asn Ile Val Thr Gly Gly Glu Trp Asn Gly ValGAA AAG GCA GCA AGA GAT CTA GAT TTG TCA AAT GCA AAG GAA ACG TTT240Glu Lys Ala Ala Arg Asp Leu Asp Leu Ser Asn Ala Lys Glu Thr Phe950 955 960AAC CCA TTG GTA GCT GCT TTT CCA AGT GTG AAT GTT AGT ATG GAA AAG288Asn Pro Leu Val Ala Ala Phe Pro Ser Val Asn Val Ser Met Glu Lys965 970 975 980GTG ATA TTA TCA CCA AAT GAA AAT TTA TCC AAT AGT GTA GAG TCT CAT336Val Ile Leu Ser Pro Asn Glu Asn Leu Ser Asn Ser Val Glu Ser His985 990 995TCA TCC ACG AAT TGG TCT TAT ACA AAT ACA GAA GGT GCT TCT GTT GAA384Ser Ser Thr Asn Trp Ser Tyr Thr Asn Thr Glu Gly Ala Ser Val Glu100010051010GCG GGG ATT GGA CCA AAA GGT ATT TCG TTC GGA GTT AGC GTA AAC TAT432Ala Gly Ile Gly Pro Lys Gly Ile Ser Phe Gly Val Ser Val Asn Tyr101510201025CAA CAC TCT GAA ACA GTT GCA CAA GAA TGG GGA ACA TCT ACA GGA AAT480Gln His Ser Glu Thr Val Ala Gln Glu Trp Gly Thr Ser Thr Gly Asn103010351040ACT TCG CAA TTC AAT ACG GCT TCA GCG GGA TAT TTA AAT GCA AAT GTT528Thr Ser Gln Phe Asn Thr Ala Ser Ala Gly Tyr Leu Asn Ala Asn Val1045105010551060CGA TAT AAC AAT GTA GGA ACT GGT GCC ATC TAC GAT GTA AAA CCT ACA576Arg Tyr Asn Asn Val Gly Thr Gly Ala Ile Tyr Asp Val Lys Pro Thr106510701075ACA AGT TTT GTA TTA AAT AAC GAT ACT ATC GCA ACT ATT ACG GCG AAA624Thr Ser Phe Val Leu Asn Asn Asp Thr Ile Ala Thr Ile Thr Ala Lys108010851090TCT AAT TCT ACA GCC TTA AAT ATA TCT CCT GGA GAA AGT TAC CCG AAA672Ser Asn Ser Thr Ala Leu Asn Ile Ser Pro Gly Glu Ser Tyr Pro Lys109511001105AAA GGA CAA AAT GGA ATC GCA ATA ACA TCA ATG GAT GAT TTT AAT TCC720Lys Gly Gln Asn Gly Ile Ala Ile Thr Ser Met Asp Asp Phe Asn Ser111011151120CAT CCG ATT ACA TTA AAT AAA AAA CAA GTA GAT AAT CTG CTA AAT AAT768His Pro Ile Thr Leu Asn Lys Lys Gln Val Asp Asn Leu Leu Asn Asn1125113011351140AAA CCT ATG ATG TTG GAA ACA AAC CAA ACA GAT GGT GTT TAT AAG ATA816Lys Pro Met Met Leu Glu Thr Asn Gln Thr Asp Gly Val Tyr Lys Ile114511501155AAA GAT ACA CAT GGA AAT ATA GTA ACT GGC GGA GAA TGG AAT GGT GTC864Lys Asp Thr His Gly Asn Ile Val Thr Gly Gly Glu Trp Asn Gly Val
116011651170ATA CAA CAA ATC AAG GCT AAA ACA GCG TCT ATT ATT GTG GAT GAT GGG 912Ile Gln Gln Ile Lys Ala Lys Thr Ala Ser Ile Ile Val Asp Asp Gly117511801185GAA CGT GTA GCA GAA AAA CGT GTA GCG GCA AAA GAT TAT GAA AAT CCA 960Glu Arg Val Ala Glu Lys Arg Val Ala Ala Lys Asp Tyr Glu Asn Pro119011951200GAA GAT AAA ACA CCG TCT TTA ACT TTA AAA GAT GCC CTG AAG CTT TCA1008Glu Asp Lys Thr Pro Ser Leu Thr Leu Lys Asp Ala Leu Lys Leu Ser1205121012151220TAT CCA GAT GAA ATA AAA GAA ATA GAG GGA TTA TTA TAT TAT AAA AAC1056Tyr Pro Asp Glu Ile Lys Glu Ile Glu Gly Leu Leu Tyr Tyr Lys Asn122512301235AAA CCG ATA TAC GAA TCG AGC GTT ATG ACT TAC TTA GAT GAA AAT ACA1104Lys Pro Ile Tyr Glu Ser Ser Val Met Thr Tyr Leu Asp Glu Asn Thr124012451250GCA AAA GAA GTG ACC AAA CAA TTA AAT GAT ACC ACT GGG AAA TTT AAA1152Ala Lys Glu Val Thr Lys Gln Leu Asn Asp Thr Thr Gly Lys Phe Lys125512601265GAT GTA AGT CAT TTA TAT GAT GTA AAA CTG ACT CCA AAA ATG AAT GTT1200Asp Val Ser His Leu Tyr Asp Val Lys Leu Thr Pro Lys Met Asn Val127012751280ACA ATC AAA TTG TCT ATA CTT TAT GAT AAT GCT GAG TCT AAT GAT AAC1248Thr Ile Lys Leu Ser Ile Leu Tyr Asp Asn Ala Glu Ser Asn Asp Asn1285129012951300TCA ATT GGT AAA TGG ACA AAC ACA AAT ATT GTT TCA GGT GGA AAT AAC1296Ser Ile Gly Lys Trp Thr Asn Thr Asn Ile Val Ser Gly Gly Asn Asn130513101315GGA AAA AAA CAA TAT TCT TCT AAT AAT CCG GAT GCT AAT TTG ACA TTA1344Gly Lys Lys Gln Tyr Ser Ser Asn Asn Pro Asp Ala Asn Leu Thr Leu132013251330AAT ACA GAT GCT CAA GAA AAA TTA AAT AAA AAT CGT GAC TAT TAT ATA1392Asn Thr Asp Ala Gln Glu Lys Leu Asn Lys Asn Arg Asp Tyr Tyr Ile133513401345AGT TTA TAT ATG AAG TCA GAA AAA AAC ACA CAA TGT GAG ATT ACT ATA1440Ser Leu Tyr Met Lys Ser Glu Lys Asn Thr Gln Cys Glu Ile Thr Ile135013551360GAT GGG GAG ATT TAT CCG ATC ACT ACA AAA ACA GTG AAT GTG AAT AAA1488Asp Gly Glu Ile Tyr Pro Ile Thr Thr Lys Thr Val Asn Val Asn Lys1365137013751380GAC AAT TAC AAA AGA TTA GAT ATT ATA GCT CAT AAT ATA AAA AGT AAT1536Asp Asn Tyr Lys Arg Leu Asp Ile Ile Ala His Asn Ile Lys Ser Asn138513901395CCA ATT TCT TCA CTT CAT ATT AAA ACG AAT GAT GAA ATA ACT TTA TTT 1584Pro Ile Ser Ser Leu His Ile Lys Thr Asn Asp Glu Ile Thr Leu Phe140014051410TGG GAT GAT ATT TCT ATA ACA GAT GTA GCA TCA ATA AAA CCG GAA AAT 1632Trp Asp Asp Ile Ser Ile Thr Asp Val Ala Ser Ile Lys Pro Glu Asn141514201425TTA ACA GAT TCA GAA ATT AAA CAG ATT TAT AGT AGG TAT GGT ATT AAG 1680Leu Thr Asp Ser Glu Ile Lys Gln Ile Tyr Ser Arg Tyr Gly Ile Lys143014351440TTA GAA GAT GGA ATC CTT ATT GAT AAA AAA GGT GGG ATT CAT TAT GGT 1728Leu Glu Asp Gly Ile Leu Ile Asp Lys Lys Gly Gly Ile His Tyr Gly1445145014551460GAA TTT ATT AAT GAA GCT AGT TTT AAT ATT GAA CCA TTG CCA AAT TAT 1776Glu Phe Ile Asn Glu Ala Ser Phe Asn Ile Glu Pro Leu Pro Asn Tyr146514701475GTG ACC AAA TAT GAA GTT ACT TAT AGT AGT GAG TTA GGA CCA AAC GTG 1824Val Thr Lys Tyr Glu Val Thr Tyr Ser Ser Glu Leu Gly Pro Asn Val148014851490AGP GAC ACA CTT GAA AGT GAT AAA ATT TAC AAG GAT GGG ACA ATT AAA 1872Ser Asp Thr Leu Glu Ser Asp Lys Ile Tyr Lys Asp Gly Thr Ile Lys149515001505TTT GAT TTT ACC AAA TAT AGT AAA AAT GAA CAA GGA TTA TTT TAT GAC 1920Phe Asp Phe Thr Lys Tyr Ser Lys Asn Glu Gln Gly Leu Phe Tyr Asp15101515 1520AGT GGA TTA AAT TGG GAC TTT AAA ATT AAT GCT ATT ACT TAT GAT GGT 1968Ser Gly Leu Asn Trp Asp Phe Lys Ile Asn Ala Ile Thr Tyr Asp Gly1525153015351540AAA GAG ATG AAT GTT TTT CAT AGA TAT AAT AAA TAG 2004Lys Glu Met Asn Val Phe His Arg Tyr Asn Lys15451550(2)关于SEQ ID NO:7的信息(ⅰ)序列特征(A)长度667个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:7:Met Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser Ile1 5 10 15Pro Asp Leu Trp Glu Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile Ala20 25 30Val Lys Trp Asp Asp Ser Leu Ala Ser Lys Gly Tyr Thr Lys Phe Val35 40 45Ser Asn Pro Leu Glu Ser His Thr Val Gly Asp Pro Tyr Thr Asp Tyr50 55 60Glu Lys Ala Ala Arg Asp Leu Asp Leu Ser Asn Ala Lys Glu Thr Phe65 70 75 80Asn Pro Leu Val Ala Ala Phe Pro Ser Val Asn Val Ser Met Glu Lys85 90 95Val Ile Leu Ser Pro Asn Glu Asn Leu Ser Asn Ser Val Glu Ser His100 105 110Ser Ser Thr Asn Trp Ser Tyr Thr Asn Thr Glu Gly Ala Ser Val Glu115 120 125Ala Gly Ile Gly Pro Lys Gly Ile Ser Phe Gly Val Ser Val Asn Tyr130 135 140Gln His Ser Glu Thr Val Ala Gln Glu Trp Gly Thr Ser Thr Gly Asn145 150 155 160Thr Ser Gln Phe Asn Thr Ala Ser Ala Gly Tyr Leu Asn Ala Asn Val165 170 175Arg Tyr Asn Asn Val Gly Thr Gly Ala Ile Tyr Asp Val Lys Pro Thr180 185 190Thr Ser Phe Val Leu Asn Asn Asp Thr Ile Ala Thr Ile Thr Ala Lys195 200 205Ser Asn Ser Thr Ala Leu Asn Ile Ser Pro Gly Glu Ser Tyr Pro Lys210 215 220Lys Gly Gln Asn Gly Ile Ala Ile Thr Ser Met Asp Asp Phe Asn Ser225 230 235 240His Pro Ile Thr Leu Asn Lys Lys Gln Val Asp Asn Leu Leu Asn Asn245 250 255Lys Pro Met Met Leu Glu Thr Asn Gln Thr Asp Gly Val Tyr Lys Ile260 265 270Lys Asp Thr His Gly Asn Ile Val Thr Gly Gly Glu Trp Asn Gly Val275 280 285Ile Gln Gln Ile Lys Ala Lys Thr Ala Ser Ile Ile Val Asp Asp Gly
290 295 300Glu Arg Val Ala Glu Lys Arg Val Ala Ala Lys Asp Tyr Glu Asn Pro305 310 315 320Glu Asp Lys Thr Pro Ser Leu Thr Leu Lys Asp Ala Leu Lys Leu Ser325 330 335Tyr Pro Asp Glu Ile Lys Glu Ile Glu Gly Leu Leu Tyr Tyr Lys Asn340 345 350Lys Pro Ile Tyr Glu Ser Ser Val Met Thr Tyr Leu Asp Glu Asn Thr355 360 365Ala Lys Glu Val Thr Lys Gln Leu Asn Asp Thr Thr Gly Lys Phe Lys370 375 380Asp Val Ser His Leu Tyr Asp Val Lys Leu Thr Pro Lys Met Asn Val385 390 395 400Thr Ile Lys Leu Ser Ile Leu Tyr Asp Asn Ala Glu Ser Asn Asp Asn405 410 415Ser Ile Gly Lys Trp Thr Asn Thr Asn Ile Val Ser Gly Gly Asn Asn420 425 430Gly Lys Lys Gln Tyr Ser Ser Asn Asn Pro Asp Ala Asn Leu Thr Leu435 440 445Asn Thr Asp Ala Gln Glu Lys Leu Asn Lys Asn Arg Asp Tyr Tyr Ile450 455 460Ser Leu Tyr Met Lys Ser Glu Lys Asn Thr Gln Cys Glu Ile Thr Ile465 470 475 480Asp Gly Glu Ile Tyr Pro Ile Thr Thr Lys Thr Val Asn Val Asn Lys485 490 495Asp Asn Tyr Lys Arg Leu Asp Ile Ile Ala His Asn Ile Lys Ser Asn500 505 510Pro Ile Ser Ser Leu His Ile Lys Thr Asn Asp Glu Ile Thr Leu Phe515 520 525Trp Asp Asp Ile Ser Ile Thr Asp Val Ala Ser Ile Lys Pro Glu Asn530 535 540Leu Thr Asp Ser Glu Ile Lys Gln Ile Tyr Ser Arg Tyr Gly Ile Lys545 550 555 560Leu Glu Asp Gly Ile Leu Ile Asp Lys Lys Gly Gly Ile His Tyr Gly565 570 575Glu Phe Ile Asn Glu Ala Ser Phe Asn Ile Glu Pro Leu Pro Asn Tyr580 585 590Val Thr Lys Tyr Glu Val Thr Tyr Ser Ser Glu Leu Gly Pro Asn Val595 600 605Ser Asp Thr Leu Glu Ser Asp Lys Ile Tyr Lys Asp Gly Thr Ile Lys610 615 620Phe Asp Phe Thr Lys Tyr Ser Lys Asn Glu Gln Gly Leu Phe Tyr Asp625 630 635 640Ser Gly Leu Asn Trp Asp Phe Lys Ile Asn Ala Ile Thr Tyr Asp Gly645 650 655Lys Glu Met Asn Val Phe His Arg Tyr Asn Lys660 665(2)关于SEQ ID NO:8的信息(ⅰ)序列特征(A)长度16个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型肽(ⅲ)假拟否(ⅴ)片段类型N-端(ⅵ)原始来源(A)生物蜡状芽孢杆菌(B)菌株AB78(C)特定分离物NRRL B-21058(ⅸ)特征(A)名称/键肽(B)位置1..16(D)其它信息/备注=从AB18菌株纯化的蛋白的N端序列(ⅹⅰ)序列描述SEQ ID NO:8:Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asx Gly Asp Ser Ile Pro1 5 10 15(2)关于SEQ ID NO:9的信息(ⅰ)序列特征(A)长度21个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅳ)反义否(ⅸ)特征(A)名称/键misc_feature(B)位置1..21(D)其它信息/备注=“基于SEQ ID NO:8的3到9位氨基酸的寡核苷酸探针，使用苏云金芽孢杆菌的密码子用法。”(ⅹⅰ)序列描述SEQ ID NO:9:GAAATTGATC AAGATACNGA T 21(2)关于SEQ ID NO:10的信息(ⅰ)序列特征(A)长度14个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅲ)假拟否(ⅴ)片段类型N-端(ⅵ)原始来源(A)生物苏云金芽孢杆菌(B)菌株AB88(ⅸ)特征
(A)名称/键肽(B)位置1.14(D)其它信息/备注=“称为阴离子交换组分23的蛋白的N端氨基酸序列(更小的)。”(ⅹⅰ)序列描述SEQ ID NO:10:Xaa Glu Pro Phe Val Ser Ala Xaa Xaa Xaa Gln Xaa Xaa Xaa1 5 10(2)关于SEQ ID NO:11的信息(ⅰ)序列特征(A)长度13个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构N-端(ⅵ)原始来源(A)生物苏云金芽孢杆菌(ⅹⅰ)序列描述SEQ ID NO:11:Xaa Glu Tyr Glu Asn Val Glu Pro Phe Val Ser Ala Xaa1 5 10(2)关于SEQ ID NO:12的信息(ⅰ)序列特征(A)长度14个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构N-端(ⅵ)原始来源(A)生物苏云金芽孢杆菌(ⅹⅰ)序列描述SEQ ID NO:12:Met Asn Lys Asn Asn Thr Lys Leu Pro Thr Arg Ala Leu Pro1 5 10(2)关于SEQ ID NO:13的信息(ⅰ)序列特征(A)长度15个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅲ)假拟否(ⅴ)片段类型N-端(ⅵ)原始来源(A)生物苏云金芽孢杆菌(B)菌株AB88(ⅸ)特征(A)名称/键肽(B)位置1..15(D)其它信息/备注=“对Agrotis ipsilon有活性的35kDa VIP的N端氨基酸序列。”(ⅹⅰ)序列描述SEQ ID NO:13:Ala Leu Ser Glu Asn Thr Gly Lys Asp Gly Gly Tyr Ile Val Pro1 5 10 15(2)关于SEQ ID NO:14的信息(ⅰ)序列特征(A)长度9个氨基酸(B)类型氨基酸(C)链型单链
(D)拓扑结构N-端(ⅵ)原始来源(A)生物苏云金芽孢杆菌(ⅹⅰ)序列描述SEQ ID NO:14:Met Asp Asn Asn Pro Asn Ile Asn Glu1 5(2)关于SEQ ID NO:15的信息(ⅰ)序列特征(A)长度9个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅲ)假拟否(ⅴ)片段类型N-端(ⅸ)特征(A)名称/键肽(B)位置1..9(D)其它信息/备注=“80kDa δ-内毒素的N端序列。”(ⅹⅰ)序列描述SEQ ID NO:15:Met Asp Asn Asn Pro Asn Ile Asn Glu1 5(2)关于SEQ ID NO:16的信息(ⅰ)序列特征(A)长度11个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型肽(ⅲ)假拟否(ⅴ)片段类型N-端(ⅵ)原始来源(A)生物苏云金芽孢杆菌(ⅸ)特征(A)名称/键肽(B)位置1..11(D)其它信息/备注=“60kDa δ-内毒素的N端序列。”(ⅹⅰ)序列描述SEQ ID NO:16:Met Asn Val Leu Asn Ser Gly Arg Thr Thr Ile1 5 10(2)关于SEQ ID NO:17的信息(ⅰ)序列特征(A)长度2655个核苷酸(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅳ)反义否(ⅸ)特征(A)名称/键misc_feathure(B)位置1..2652(D)其它信息/备注=“来自AB78的编码100kd VIP1A(a)蛋白的玉米最佳化DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:17:ATGAAGAACA TGAAGAAGAA GCTGGCCAGC GTGGTGACCT GCACCCTGCT GGCCCCCATG 60TTCCTGAACG GCAACGTGAA CGCCGTGTAC GCCGACAGCA AGACCAACCA GATCAGCACC 120ACCCAGAAGA ACCAGCAGAA GGAGATGGAC CGCAAGGGCC TGCTGGGCTA CTACTTCAAG 180GGCAAGGACT TCAGCAACCT GACCATGTTC GCCCCCACGC GTGACAGCAC CCTGATCTAC 240GACCAGCAGA CCGCCAACAA GCTGCTGGAC AAGAAGCAGC AGGAGTACCA GAGCATCCGC 300TGGATCGGCC TGATCCAGAG CAAGGAGACC GGCGACTTCA CCTTCAACCT GAGCGAGGAC 360GAGCAGGCCA TCATCGAGAT CAACGGCAAG ATCATCAGCA ACAAGGGCAA GGAGAAGCAG 420GTGGTGCACC TGGAGAAGGG CAAGCTGGTG CCCATCAAGA TCGAGTACCA GAGCGACACC 480AAGTTCAACA TCGACAGCAA GACCTTCAAG GAGCTGAAGC TTTTCAAGAT CGACAGCCAG 540AACCAGCCCC AGCAGGTGCA GCAGGACGAG CTGCGCAACC CCGAGTTCAA CAAGAAGGAG 600AGCCAGGAGT TCCTGGCCAA GCCCAGCAAG ATCAACCTGT TCACCCAGCA GATGAAGCGC 660GAGATCGACG AGGACACCGA CACCGACGGC GACAGCATCC CCGACCTGTG GGAGGAGAAC 720GGCTACACCA TCCAGAACCG CATCGCCGTG AAGTGGGACG ACAGCCTGGC TAGCAAGGGC 780TACACCAAGT TCGTGAGCAA CCCCCTGGAG AGCCACACCG TGGGCGACCC CTACACCGAC 840TACGAGAAGG CCGCCCGCGA CCTGGACCTG AGCAACGCCA AGGAGACCTT CAACCCCCTG 900GTGGCCGCCT TCCCCAGCGT GAACGTGAGC ATGGAGAAGG TGATCCTGAG CCCCAACGAG 960AACCTGAGCA ACAGCGTGGA GAGCCACTCG AGCACCAACT GGAGCTACAC CAACACCGAG 1020GGCGCCAGCG TGGAGGCCGG CATCGGTCCC AAGGGCATCA GCTTCGGCGT GAGCGTGAAC 1080TACCAGCACA GCGAGACCGT GGCCCAGGAG TGGGGCACCA GCACCGGCAA CACCAGCCAG 1140TTCAACACCG CCAGCGCCGG CTACCTGAAC GCCAACGTGC GCTACAACAA CGTGGGCACC 1200GGCGCCATCT ACGACGTGAA GCCCACCACC AGCTTCGTGC TGAACAACGA CACCATCGCC 1260ACCATCACCG CCAAGTCGAA TTCCACCGCC CTGAACATCA GCCCCGGCGA GAGCTACCCC 1320AAGAAGGGCC AGAACGGCAT CGCCATCACC AGCATGGACG ACTTCAACAG CCACCCCATC 1380ACCCTGAACA AGAAGCAGGT GGACAACCTG CTGAACAACA AGCCCATGAT GCTGGAGACC 1440AACCAGACCG ACGGCGTCTA CAAGATCAAG GACACCCACG GCAACATCGT GACCGGCGGC 1500GAGTGGAACG GCGTGATCCA GCAGATCAAG GCCAAGACCG CCAGCATCAT CGTCGACGAC 1560GGCGAGCGCG TGGCCGAGAA GCGCGTGGCC GCCAAGGACT ACGAGAACCC CGAGGACAAG 1620ACCCCCAGCC TGACCCTGAA GGACGCCCTG AAGCTGAGCT ACCCCGACGA GATCAAGGAG 1680ATCGAGGGCC TGCTGTACTA CAAGAACAAG CCCATCTACG AGAGCAGCGT GATGACCTAT 1740CTAGACGAGA ACACCGCCAA GGAGGTGACC AAGCAGCTGA ACGACACCAC CGCCAAGTTC 1800AAGGACGTGA GCCACCTGTA CCACGTGAAG CTGACCCCCA AGATGAACGT GACCATCAAG 1860CTGAGCATCC TGTACGACAA CGCCGAGAGC AACGACAACA GCATCGGCAA GTGCACCAAC 1920ACCAACATCG TGAGCGGCGG CAACAACGGC AAGAAGCAGT ACAGCAGCAA CAACCCCGAC 1980GCCAACCTGA CCCTGAACAC CGACGCCCAG GAGAAGCTGA ACAAGAACCG CGACTACTAC 2040ATCAGCCTGT ACATGAAGAG CGAGAAGAAC ACCCAGTGCG ACATCACCAT CGACGGCGAG 2100ATATACCCCA TCACCACCAA GACCGTGAAC GTGAACAAGG ACAACTACAA GCGCCTGGAC 2160ATCATCGCCC ACAACATCAA GAGCAACCCC ATCAGCACCC TGCACATCAA GACCAACGAC 2220GAGATCACCC TGTTCTGGGA CGACATATCG ATTACCGACG TCGCCAGCAT CAAGCCCGAG 2280AACCTGACCG ACAGCGAGAT CAAGCAGATA TACAGTCGCT ACGGCATCAA GCTGGAGGAC 2340GGCATCCTGA TCGACAAGAA GGGCGGCATC CACTACGGCG AGTTCATCAA CGAGGCCAGC 2400TTCAACATCG AGCCCCTGCA GAACTACGTG ACCAAGTACG AGGTGACCTA CAGCAGCGAG 2460CTGGGCCCCA ACGTGAGCGA CACCCTGGAG AGCGACAAGA TTTACAAGGA CGGCACCATC 2520AAGTTCGACT TCACCAAGTA CAGCAAGAAC GAGCAGGGCC TGTTCTACGA CAGCGGCCTG 2580AACTGGGACT TCAAGATCAA CGCCATCACC TACGACGGCA AGGAGATGAA CGTGTTCCAC 2640CGCTACAACA AGTAG 2655(2)关于SEQ ID NO:18的信息(ⅰ)序列特征(A)长度2004个核苷酸(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅵ)反义否(ⅸ)特征(A)名称/键misc_feathure(B)位置1..2004(D)其它信息/备注=“来自AB78的编码80kd VIP1A(a)蛋白的玉米最佳化DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:18:ATGAAGCGCG AGATCGACGA GGACACCGAC ACCGACGGCG ACAGCATCCC CGACCTGTGG60GAGGAGAACG GCTACACCAT CCAGAACCGC ATCGCCGTGA AGTGGGACGA CAGCCTGGCT 120AGCAAGGGCT ACACCAAGTT CGTGAGCAAC CCCCTGGAGA GCCACACCGT GGGCGACCCC 180TACACCGACT ACGAGAAGGC CGCCCGCGAC CTGGACCTGA GCAACGCCAA GGAGACCTTC 240AACCCCCTGG TGGCCGCCTT CCCCAGCGTG AACGTGAGCA TGGAGAAGGT GATCCTGAGC 300CCCAACGAGA ACCTGAGCAA CAGCGTGGAG AGCCACTCGA GCACCAACTG GAGCTACACC 360AACACCGAGG GCGCCAGCGT GGAGGCCGGC ATCGGTCCCA AGGGCATCAG CTTCGGCGTG 420AGCGTGAACT ACCAGCACAG CGAGACCGTG GCCCAGGAGT GGGGCACCAG CACCGGCAAC 480ACCAGCCAGT TCAACACCGC CAGCGCCGGC TACCTGAACG CCAACGTGCG CTACAACAAC 540GTGGGCACCG GCGCCATCTA CGACGTGAAG CCCACCACCA GCTTCGTGCT GAACAACGAC 600ACCATCGCCA CCATCACCGC CAAGTCGAAT TCCACCGCCC TGAACATCAG CCCCGGCGAG 660AGCTACCCCA AGAAGGGCCA GAACGGCATC GCCATCACCA GCATGGACGA CTTCAACAGC 720CACCCCATCA CCCTGAACAA GAAGCAGGTG GACAACCTGC TGAACAACAA GCCCATGATG 780CTGGAGACCA ACCAGACCGA CGGCGTCTAC AAGATCAAGG ACACCCACGG CAACATCGTG 840ACCGGCGGCG AGTGGAACGG CGTGATCCAG CAGATCAAGG CCAAGACCGC CAGCATCATC 900GTCGACGACG GCGAGCGCGT GGCCGAGAAG CGCGTGGCCG CCAAGGACTA CGAGAACCCC 960GAGGACAAGA CCCCCAGCCT GACCCTGAAG GACGCCCTGA AGCTGAGCTA CCCCGACGAG 1020ATCAAGGAGA TCGAGGGCCT GCTGTACTAC AAGAACAAGC CCATCTACGA GAGCAGCGTG 1080ATGACCTATC TAGACGAGAA CACCGCCAAG GAGGTGACCA AGCAGCTGAA CGACACCACC 1140GGCAAGTTCA AGGACGTGAG CCACCTGTAC GACGTGAAGC TGACCCCCAA GATGAACGTG 1200ACCATCAAGC TGAGCATCCT GTACGACAAC GCCGAGAGCA ACGACAACAG CATCGGCAAG 1260TGGACCAACA CCAACATCGT GAGCGGCGGC AACAACGGCA AGAAGCAGTA CAGCAGCAAC 1320AACCCCGACG CCAACCTGAC CCTGAACACC GACGCCCAGG AGAAGCTGAA CAAGAACCGC 1380GACTACTACA TCAGCCTGTA CATGAAGAGC GAGAAGAACA CCCAGTGCGA GATCACCATC 1440GACGGCGAGA TATACCCCAT CACCACCAAG ACCGTGAACG TGAACAAGGA CAACTACAAG 1500CGCCTGGACA TCATCGCCCA CAACATCAAG AGCAACCCCA TCAGCAGCCT GCACATCAAG 1560ACCAACGACG AGATCACCCT GTTCTGGGAC GACATATCGA TTACCGACGT CGCCAGCATC 1620AAGCCCGAGA ACCTGACCGA CAGCGAGATC AAGCAGATAT ACAGTCGCTA CGGCATCAAG 1680CTGGAGGACG GCATCCTGAT CGACAAGAAG GGCGGCATCC ACTACGGCGA GTTCATCAAC 1740GAGGCCAGCT TCAACATCGA GCCCCTGCAG AACTACGTGA CCAAGTACGA GGTGACCTAC 1800AGCAGCGAGC TGGGCCCCAA CGTGAGCGAC ACCCTGGAGA GCGACAAGAT TTACAAGGAC 1860GGCACCATCA AGTTCGACTT CACCAAGTAC AGCAAGAACG AGCAGGGCCT GTTCTACGAC 1920AGCGGCCTGA ACTGGGACTT CAAGATCAAC GCCATCACCT ACGACGGCAA GGAGATGAAC 1980GTGTTCCACC GCTACAACAA GTAG 2004(2)关于SEQ ID NO:19的信息(ⅰ)序列特征(A)长度4074个核苷酸(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅸ)特征(A)名称/键CDS(B)位置1..1386(D)其它信息/产品=“来自Btt的VIP2A(b)”(ⅸ)特征(A)名称/键CDS(B)位置1394..3895(D)其它信息/产品=“来自Btt的VIP1A(b)”(ⅸ)特征(A)名称/键misc_feature(B)位置1..4074(D)其它信息/备注=“来自Btt的含编码VIP1A(b)和VIP2A(b)的基因的克隆DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:19:ATG CAA AGA ATG GAG GGA AAG TTG TTT GTG GTG TCA AAA ACA TTA CAA 48Met Gln Arg Met Glu Gly Lys Leu Phe Val Val Ser Lys Thr Leu Gln670 675 680GTA GTT ACT AGA ACT GTA TTG CTT AGT ACA GTT TAC TCT ATA ACT TTA 96Val Val Thr Arg Thr Val Leu Leu Ser Thr Val Tyr Ser Ile Thr Leu685 690 695TTA AAT AAT GTA GTG ATA AAA GCT GAC CAA TTA AAT ATA AAT TCT CAA144Leu Asn Asn Val Val Ile Lys Ala Asp Gln Leu Asn Ile Asn Ser Gln700 705 710 715AGT AAA TAT ACT AAC TTG CAA AAT CTA AAA ATC CCT GAT AAT GCA GAG192Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Pro Asp Asn Ala Glu720 725 730GAT TTT AAA GAA GAT AAG GGG AAA GCG AAA GAA TGG GGG AAA GAG AAA240Asp Phe Lys Glu Asp Lys Gly Lys Ala Lys Glu Trp Gly Lys Glu Lys735 740 745GGG GAA GAG TGG AGG CCT CCT GCT ACT GAG AAA GGA GAA ATG AAT AAT288Gly Glu Glu Trp Arg Pro Pro Ala Thr Glu Lys Gly Glu Met Asn Asn750 755 760TTT TTA GAT AAT AAA AAT GAT ATA AAG ACC AAT TAT AAA GAA ATT ACT336Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr765 770 775TTT TCT ATG GCA GGT TCA TGT GAA GAT GAA ATA AAA GAT TTA GAA GAA384Phe Ser Met Ala Gly Ser Cys Glu Asp Glu Ile Lys Asp Leu Glu Glu780 785 790 795ATT GAT AAG ATC TTT GAT AAA GCC AAT CTC TCG AGT TCT ATT ATC ACC432Ile Asp Lys Ile Phe Asp Lys Ala Asn Leu Ser Ser Ser Ile Ile Thr800 805 810TAT AAA AAT GTG GAA CCA GCA ACA ATT GGA TTT AAT AAA TCT TTA ACA480Tyr Lys Asn Val Glu Pro Ala Thr Ile Gly Phe Asn Lys Ser Leu Thr815 820 825GAA GGT AAT ACG ATT AAT TCT GAT GCA ATG GCA CAG TTT AAA GAA CAA528Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln830 835 840TTT TTA GGT AAG GAT ATG AAG TTT GAT AGT TAT CTA GAT ACT CAT TTA576Phe Leu Gly Lys Asp Met Lys Phe Asp Ser Tyr Leu Asp Thr His Leu845 850 855ACT GCT CAA CAA GTT TCC AGT AAA AAA AGA GTT ATT TTG AAG GTT ACG624Thr Ala Gln Gln Val Ser Ser Lys Lys Arg Val Ile Leu Lys Val Thr860 865 870 875GTT CCG AGT GGG AAA GGT TCT ACT ACT CCA ACA AAA GCA GGT GTC ATT672Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile880 885 890TTA AAC AAT AAT GAA TAC AAA ATG CTC ATT GAT AAT GGG TAT GTG CTC720Leu Asn Asn Asn Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Val Leu895 900 905CAT GTA GAT AAG GTA TCA AAA GTA GTA AAA AAA GGG ATG GAG TGC TTA768His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Met Glu Cys Leu910 915 920CAA GTT GAA GGG ACT TTA AAA AAG AGT CTC GAC TTT AAA AAT GAT ATA816Gln Val Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile925 930 935AAT GCT GAA GCG CAT AGC TGG GGG ATG AAA ATT TAT GAA GAC TGG GCT864Asn Ala Glu Ala His Ser Trp Gly Met Lys Ile Tyr Glu Asp Trp Ala940 945 950 955AAA AAT TTA ACC GCT TCG CAA AGG GAA GCT TTA GAT GGG TAT GCT AGG912Lys Asn Leu Thr Ala Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg960 965 970CAA GAT TAT AAA GAA ATC AAT AAT TAT TTG CGC AAT CAA GGC GGG AGT960Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser975 980 985GGA AAT GAA AAG CTG GAT GCC CAA TTA AAA AAT ATT TCT GAT GCT TTA 1008Gly Asn Glu Lys Leu Asp Ala Gln Leu Lys Asn Ile Ser Asp Ala Leu990 995 1000GGG AAG AAA CCC ATA CCA GAA AAT ATT ACC GTG TAT AGA TGG TGT GGC 1056Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly100510101015ATG CCG GAA TTT GGT TAT CAA ATT AGT GAT CCG TTA CCT TCT TTA AAA 1104Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys1020102510301035GAT TTT GAA GAA CAA TTT TTA AAT ACA ATT AAA GAA GAC AAA GGG TAT 1152Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr104010451050ATG AGT ACA AGC TTA TCG AGT GAA CGT CTT GCA GCT TTT GGA TCT AGA 1200Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg105510601065AAA ATT ATA TTA CGC TTA CAA GTT CCG AAA GGA AGT ACG GGG GCG TAT 1248Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr107010751080TTA AGT GCC ATT GGT GGA TTT GCA AGT GAA AAA GAG ATC CTA CrT GAT 1296Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp108510901095AAA GAT AGT AAA TAT CAT ATT GAT AAA GCA ACA GAG GTA ATC ATT AAA 1344Lys Asp Ser Lys Tyr His Ile Asp Lys Ala Thr Glu Val Ile Ile Lys1100110511101115GGT GTT AAG CGA TAT GTA GTG GAT GCA ACA TTA TTA ACA AAT 1386Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn11201125TAAGGAG ATG AAA AAT ATG AAG AAA AAG TTA GCA AGT GTr GTA ACC TGT 1435Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys1 5 10ATG TTA TTA GCT CCT ATG TTT TTG AAT GGA AAT GTG AAT GCT GTT AAC 1483Met Leu Leu Ala Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Asn15 20 25 30GCG GAT AGT AAA ATA AAT CAG ATT TCT ACA ACG CAG GAA AAC CAA CAG 1531Ala Asp Ser Lys Ile Asn Gln Ile Ser Thr Thr Gln Glu Asn Gln Gln35 40 45AAA GAG ATG GAC CGA AAG GGA TTA TTG GGA TAT TAT TTC AAA GGA AAA 1579Lys Glu Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys50 55 60GAT TTT AAT AAT CTT ACT ATG TTT GCA CCG ACA CGT GAT AAT ACC CTT 1627Asp Phe Asn Asn Leu Thr Met Phe Ala Pro Thr Arg Asp Asn Thr Leu65 70 75ATG TAT GAC CAA CAA ACA GCG AAT GCA TTA TTA GAT AAA AAA CAA CAA 1675Met Tyr Asp Gln Gln Thr Ala Asn Ala Leu Leu Asp Lys Lys Gln Gln80 85 90GAA TAT CAG TCC ATT CGT TGG ATT GGT TTG ATT CAG CGT AAA GAA ACG 1723Glu Tyr Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln Arg Lys Glu Thr95 100 105 110GGC GAT TTC ACA TTT AAC TTA TCA AAG GAT GAA CAG GCA ATT ATA GAA 1771Gly Asp Phe Thr Phe Asn Leu Ser Lys Asp Glu Gln Ala Ile Ile Glu115 120 125ATC GAT GGG AAA ATC ATT TCT AAT AAA GGG AAA GAA AAG CAA GTT GTC 1819Ile Asp Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val130 135 140CAT TTA GAA AAA GAA AAA TTA GTT CCA ATC AAA ATA GAG TAT CAA TCA 1867His Leu Glu Lys Glu Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser145 150 155GAT ACG AAA TTT AAT ATT GAT AGT AAA ACA TTT AAA GAA CTT AAA TTA 1915Asp Thr Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu160 165 170TTT AAA ATA GAT AGT CAA AAC CAA TCT CAA CAA GTT CAA CTG AGA AAC 1963Phe Lys Ile Asp Ser Gln Asn Gln Ser Gln Gln Val Gln Leu Arg Asn175 180 185 190CCT GAA TTT AAC AAA AAA GAA TCA CAG GAA TTT TTA GCA AAA GCA TCA 2011Pro Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Ala Ser195 200 205AAA ACA AAC CTT TTT AAG CAA AAA ATG AAA AGA GAT ATT GAT GAA GAT 2059Lys Thr Asn Leu Phe Lys Gln Lys Met Lys Arg Asp Ile Asp Glu Asp210 215 220ACG GAT ACA GAT GGA GAC TCC ATT CCT GAT CTT TGG GAA GAA AAT GGG 2107Thr Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn Gly225 230 235TAC ACG ATT CAA AAT AAA GTT GCT GTC AAA TGG GAT GAT TCG CTA GCA 2155Tyr Thr Ile Gln Asn Lys Val Ala Val Lys Trp Asp Asp Ser Leu Ala240 245 250AGT AAG GGA TAT ACA AAA TTT GTT TCG AAT CCA TTA GAC AGC CAC ACA 2203Ser Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Asp Ser His Thr255 260 265 270GTT GGC GAT CCC TAT ACT GAT TAT GAA AAG GCC GCA AGG GAT TTA GAT 2251Val Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg Asp Leu Asp275 280 285TTA TCA AAT GCA AAG GAA ACG TTC AAC CCA TTG GTA GCT GCT TTT CCA 2299Leu Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe Pro290 295 300AGT GTG AAT GTT AGT ATG GAA AAG GTG ATA TTA TCA CCA AAT GAA AAT 2347Ser Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu Asn305 310 315TTA TCC AAT AGT GTA GAG TCT CAT TCA TCC ACG AAT TGG TCT TAT ACG 2395Leu Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr Thr320 325 330AAT ACA GAA GGA GCT TCC ATT GAA GCT GGT GGC GGT CCA TTA GGC CTT 2443Asn Thr Glu Gly Ala Ser Ile Glu Ala Gly Gly Gly Pro Leu Gly Leu335 340 345 350TCT TTT GGC GTG AGT GTT ACT TAT CAA CAC TCT GAA ACA GTT GCA CAA 2491Ser Phe Gly Val Ser Val Thr Tyr Gln His Ser Glu Thr Val Ala Gln355 360 365GAA TGG GGA ACA TCT ACA GGA AAT ACT TCA CAA TTC AAT ACG GCT TCA 2539Glu Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala Ser370 375 380GCG GGA TAT TTA AAT GCA AAT GTT CGG TAT AAC AAT GTA GGG ACT GGT 2587Ala Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr Gly385 390 395GCC ATC TAT GAT GTA AAA CCT ACA ACA AGT TTT GTA TTA AAT AAC AAT 2635Ala Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn Asn400 405 410ACC ATC GCA ACG ATT ACA GCA AAA TCA AAT TCA ACA GCT TTA CGT ATA 2683Thr Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Arg Ile415 420 425 430TCT CCG GGG GAT AGT TAT CCA GAA ATA GGA GAA AAC GCT ATT GCG ATT 2731Ser Pro Gly Asp Ser Tyr Pro Glu Ile Gly Glu Asn Ala Ile Ala Ile435 440 445ACA TCT ATG GAT GAT TTT AAT TCT CAT CCA ATT ACA TTA AAT AAA CAA 2779Thr Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys Gln
450 455 460CAG GTA AAT CAA TTG ATA AAT AAT AAG CCA ATT ATG CTA GAG ACA GAC 2827Gln Val Asn Gln Leu Ile Asn Asn Lys Pro Ile Met Leu Glu Thr Asp465 470 475CAA ACA GAT GGT GTT TAT AAA ATA AGA GAT ACA CAT GGA AAT ATT GTA 2875Gln Thr Asp Gly Val Tyr Lys Ile Arg Asp Thr His Gly Asn Ile Val480 485 490ACT GGT GGA GAA TGG AAT GGT GTA ACA CAA CAA ATT AAA GCA AAA ACA 2923Thr Gly Gly Glu Trp Asn Gly Val Thr Gln Gln Ile Lys Ala Lys Thr495 500 505 510GCG TCT ATT ATT GTG GAT GAC GGG AAA CAG GTA GCA GAA AAA CGT GTG 2971Ala Ser Ile Ile Val Asp Asp Gly Lys Gln Val Ala Glu Lys Arg Val515 520 525GCG GCA AAA GAT TAT GGT CAT CCA GAA GAT AAA ACA CCA CCT TTA ACT 3019Ala Ala Lys Asp Tyr Gly His Pro Glu Asp Lys Thr Pro Pro Leu Thr530 535 540TTA AAA GAT ACC CTG AAG CTT TCA TAC CCA GAT GAA ATA AAA GAA ACT 3067Leu Lys Asp Thr Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu Thr545 550 555AAT GGA TTG TTG TAC TAT GAT GAC AAA CCA ATC TAT GAA TCG AGT GTC 3115Asn Gly Leu Leu Tyr Tyr Asp Asp Lys Pro Ile Tyr Glu Ser Ser Val560 565 570ATG ACT TAT CTG GAT GAA AAT ACG GCA AAA GAA GTC AAA AAA CAA ATA 3163Met Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Lys Lys Gln Ile575 580 585 590AAT GAT ACA ACC GGA AAA TTT AAG GAT GTA AAT CAC TTA TAT GAT GTA 3211Asn Asp Thr Thr Gly Lys Phe Lys Asp Val Asn His Leu Tyr Asp Val595 600 605AAA CTG ACT CCA AAA ATG AAT TTT ACG ATT AAA ATG GCT TCC TTG TAT 3259Lys Leu Thr Pro Lys Met Asn Phe Thr Ile Lys Met Ala Ser Leu Tyr610 615 620GAT GGG GCT GAA AAT AAT CAT AAC TCT TTA GGA ACC TGG TAT TTA ACA 3307Asp Gly Ala Glu Asn Asn His Asn Ser Leu Gly Thr Trp Tyr Leu Thr625 630 635TAT AAT GTT GCT GGT GGA AAT ACT GGG AAG AGA CAA TAT CGT TCA GCT 3355Tyr Asn Val Ala Gly Gly Asn Thr Gly Lys Arg Gln Tyr Arg Ser Ala640 645 650CAT TCT TGT GCA CAT GTA GCT CTA TCT TCA GAA GCG AAA AAG AAA CTA 3403His Ser Cys Ala His Val Ala Leu Ser Ser Glu Ala Lys Lys Lys Leu655 660 665 670AAT CAA AAT GCG AAT TAC TAT CTT AGC ATG TAT ATG AAG GCT GAT TCT 3451Asn Gln Asn Ala Asn Tyr Tyr Leu Ser Met Tyr Met Lys Ala Asp Ser675 680 685ACT ACG GAA CCT ACA ATA GAA GTA GCT GGG GAA AAA TCT GCA ATA ACA 3499Thr Thr Glu Pro Thr Ile Glu Val Ala Gly Glu Lys Ser Ala Ile Thr690 695 700AGT AAA AAA GTA AAA TTA AAT AAT CAA AAT TAT CAA AGA GTT GAT ATT 3547Ser Lys Lys Val Lys Leu Asn Asn Gln Asn Tyr Gln Arg Val Asp Ile705 710 715TTA GTG AAA AAT TCT GAA AGA AAT CCA ATG GAT AAA ATA TAT ATA AGA 3595Leu Val Lys Asn Ser Glu Arg Asn Pro Met Asp Lys Ile Tyr Ile Arg720 725 730GGA AAT GGC ACG ACA AAT GTT TAT GGG GAT GAT GTT ACT ATC CCA GAG 3643Gly Asn Gly Thr Thr Asn Val Tyr Gly Asp Asp Val Thr Ile Pro Glu735 740 745 750GTA TCA GCT ATA AAT CCG GCT AGT CTA TCA GAT GAA GAA ATT CAA GAA 3691Val Ser Ala Ile Asn Pro Ala Ser Leu Ser Asp Glu Glu Ile Gln Glu755 760 765ATA TTT AAA GAC TCA ACT ATT GAA TAT GGA AAT CCT AGT TTC GTT GCT 3739Ile Phe Lys Asp Ser Thr Ile Glu Tyr Gly Asn Pro Ser Phe Val Ala770 775 780GAT GCC GTA ACA TTT AAA AAT ATA AAA CCT TTA CAA AAT TAT GTA AAG 3787Asp Ala Val Thr Phe Lys Asn Ile Lys Pro Leu Gln Asn Tyr Val Lys785 790 795GAA TAT GAA ATA TAT CAT AAA TCT CAT CGA TAT GAA AAG AAA ACG GTC 3835Glu Tyr Glu Ile Tyr His Lys Ser His Arg Tyr Glu Lys Lys Thr Val800 805 810TTT GAT ATC ATG GGT GTT CAT TAT GAG TAT AGT ATA GCT AGG GAA CAA 3883Phe Asp Ile Met Gly Val His Tyr Glu Tyr Ser Ile Ala Arg Glu Gln815 820 825 830AAG AAA GCC GCA TAATTTTTAAA AATAAAACTC GTTAGAGTTT ATTTTAGCATG3935Lys Lys Ala AlaGTATTTTTAA GAATAATCAA TATGTTGAAC CGTTTGTAGC TGTTTTGGAA GGGAATTTCA3995TTTTATTTGG TCTCTTAAGT TGATGGGCAT GGGATATGTT CAGCATCCAA GCGTTTNGGG4055GGTTANAAAA TCCAATTTT 4074(2)关于SEQ ID NO:20的信息(ⅰ)序列特征(A)长度462个氨基酸
(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:20:Met Gln Arg Met Glu Gly Lys Leu Phe Val Val Ser Lys Thr Leu Gln1 5 10 15Val Val Thr Arg Thr Val Leu Leu Ser Thr Val Tyr Ser Ile Thr Leu20 25 30Leu Asn Asn Val Val Ile Lys Ala Asp Gln Leu Asn Ile Asn Ser Gln35 40 45Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Pro Asp Asn Ala Glu50 55 60Asp Phe Lys Glu Asp Lys Gly Lys Ala Lys Glu Trp Gly Lys Glu Lys65 70 75 80Gly Glu Glu Trp Arg Pro Pro Ala Thr Glu Lys Gly Glu Met Asn Asn85 90 95Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr100 105 110Phe Ser Met Ala Gly Ser Cys Glu Asp Glu Ile Lys Asp Leu Glu Glu115 120 125Ile Asp Lys Ile Phe Asp Lys Ala Asn Leu Ser Ser Ser Ile Ile Thr130 135 140Tyr Lys Asn Val Glu Pro Ala Thr Ile Gly Phe Asn Lys Ser Leu Thr145 150 155 160Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln165 170 175Phe Leu Gly Lys Asp Met Lys Phe Asp Ser Tyr Leu Asp Thr His Leu180 185 190Thr Ala Gln Gln Val Ser Ser Lys Lys Arg Val Ile Leu Lys Val Thr195 200 205Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile210 215 220Leu Asn Asn Asn Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Val Leu225 230 235 240His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Met Glu Cys Leu245 250 255Gln Val Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile
260 265 270Asn Ala Glu Ala His Ser Trp Gly Met Lys Ile Tyr Glu Asp Trp Ala275 280 285Lys Asn Leu Thr Ala Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg290 295 300Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser305 310 315 320Gly Asn Glu Lys Leu Asp Ala Gln Leu Lys Asn Ile Ser Asp Ala Leu325 330 335Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly340 345 350Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys355360 365Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr370 375 380Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg385 390 395 400Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr405 410 415Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp420 425 430Lys Asp Ser Lys Tyr His Ile Asp Lys Ala Thr Glu Val Ile Ile Lys435 440 445Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn450 455 460(2)关于SEQ ID NO:21的信息(ⅰ)序列特征(A)长度834个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:21:Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Met Leu1 5 10 15Leu Ala Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Asn Ala Asp20 25 30Ser Lys Ile Asn Gln Ile Ser Thr Thr Gln Glu Asn Gln Gln Lys Glu35 40 45Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe50 55 60Asn Asn Leu Thr Met Phe Ala Pro Thr Arg Asp Asn Thr Leu Met Tyr65 70 75 80Asp Gln Gln Thr Ala Asn Ala Leu Leu Asp Lys Lys Gln Gln Glu Tyr85 90 95Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln Arg Lys Glu Thr Gly Asp100 105 110Phe Thr Phe Asn Leu Ser Lys Asp Glu Gln Ala Ile Ile Glu Ile Asp115 120 125Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val His Leu130 135 140Glu Lys Glu Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser Asp Thr145 150 155 160Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu Phe Lys165 170 175Ile Asp Ser Gln Asn Gln Ser Gln Gln Val Gln Leu Arg Asn Pro Glu180 185 190Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Ala Ser Lys Thr195 200 205Asn Leu Phe Lys Gln Lys Met Lys Arg Asp Ile Asp Glu Asp Thr Asp210 215 220Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn Gly Tyr Thr225 230 235 240Ile Gln Asn Lys Val Ala Val Lys Trp Asp Asp Ser Leu Ala Ser Lys245 250 255Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Asp Ser His Thr Val Gly260 265 270Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg Asp Leu Asp Leu Ser275 280 285Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe Pro Ser Val290 295 300Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu Asn Leu Ser305 310 315 320Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr Thr Asn Thr325 330 335Glu Gly Ala Ser Ile Glu Ala Gly Gly Gly Pro Leu Gly Leu Ser Phe340 345 350Gly Val Ser Val Thr Tyr Gln His Ser Glu Thr Val Ala Gln Glu Trp355 360 365Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala Ser Ala Gly370 375 380Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr Gly Ala Ile385 390 395 400Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn Asn Thr Ile405 410 415Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Arg Ile Ser Pro420 425 430Gly Asp Ser Tyr Pro Glu Ile Gly Glu Asn Ala Ile Ala Ile Thr Ser435 440 445Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys Gln Gln Val450 455 460Asn Gln Leu Ile Asn Asn Lys Pro Ile Met Leu Glu Thr Asp Gln Thr465 470 475 480Asp Gly Val Tyr Lys Ile Arg Asp Thr His Gly Asn Ile Val Thr Gly485 490 495Gly Glu Trp Asn Gly Val Thr Gln Gln Ile Lys Ala Lys Thr Ala Ser500 505 510Ile Ile Val Asp Asp Gly Lys Gln Val Ala Glu Lys Arg Val Ala Ala515 520 525Lys Asp Tyr Gly His Pro Glu Asp Lys Thr Pro Pro Leu Thr Leu Lys530 535 540Asp Thr Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu Thr Asn Gly545 550 555 560Leu Leu Tyr Tyr Asp Asp Lys Pro Ile Tyr Glu Ser Ser Val Met Thr565 570 575Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Lys Lys Gln Ile Asn Asp580 585 590Thr Thr Gly Lys Phe Lys Asp Val Asn His Leu Tyr Asp Val Lys Leu595 600 605Thr Pro Lys Met Asn Phe Thr Ile Lys Met Ala Ser Leu Tyr Asp Gly
610 615 620Ala Glu Asn Asn His Asn Ser Leu Gly Thr Trp Tyr Leu Thr Tyr Asn625 630 635 640Val Ala Gly Gly Asn Thr Gly Lys Arg Gln Tyr Arg Ser Ala His Ser645 650 655Cys Ala His Val Ala Leu Ser Ser Glu Ala Lys Lys Lys Leu Asn Gln660 665 670Asn Ala Asn Tyr Tyr Leu Ser Met Tyr Met Lys Ala Asp Ser Thr Thr675 680 685Glu Pro Thr Ile Glu Val Ala Gly Glu Lys Ser Ala Ile Thr Ser Lys690 695 700Lys Val Lys Leu Asn Asn Gln Asn Tyr Gln Arg Val Asp Ile Leu Val705 710 715 720Lys Asn Ser Glu Arg Asn Pro Met Asp Lys Ile Tyr Ile Arg Gly Asn725 730 735Gly Thr Thr Asn Val Tyr Gly Asp Asp Val Thr Ile Pro Glu Val Ser740 745 750Ala Ile Asn Pro Ala Ser Leu Ser Asp Glu Glu Ile Gln Glu Ile Phe755 760 765Lys Asp Ser Thr Ile Glu Tyr Gly Asn Pro Ser Phe Val Ala Asp Ala770 775 780Val Thr Phe Lys Asn Ile Lys Pro Leu Gln Asn Tyr Val Lys Glu Tyr785 790 795 800Glu Ile Tyr His Lys Ser His Arg Tyr Glu Lys Lys Thr Val Phe Asp805 810 815Ile Met Gly Val His Tyr Glu Tyr Ser Ile Ala Arg Glu Gln Lys Lys820 825 830Ala Ala(2)关于SEQ ID NO:22的信息(ⅰ)序列特征(A)长度4041个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅸ)特征(A)名称/键CDS(B)位置1..4038(D)其它信息/产品=“VIP1A(a)和VIP2A(a)融合产物”(ⅹⅰ)序列描述SEQ ID NO:22:ATG AAA AGA ATG GAG GGA AAG TTG TTT ATG GTG TCA AAA AAA TTA CAA 48Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln835 840 845 850GTA GTT ACT AAA ACT GTA TTG CTT AGT ACA GTT TTC TCT ATA TCT TTA 96Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu855 860 865TTA AAT AAT GAA GTG ATA AAA GCT GAA CAA TTA AAT ATA AAT TCT CAA 144Leu Asn Asn Glu Val Ile Lys Ala Glu Gln Leu Asn Ile Asn Ser Gln870 875 880AGT AAA TAT ACT AAC TTG CAA AAT CTA AAA ATC ACT GAC AAG GTA GAG 192Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu885 890 895GAT TTT AAA GAA GAT AAG GAA AAA GCG AAA GAA TGG GGG AAA GAA AAA 240Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys900 905 910GAA AAA GAG TGG AAA CTA ACT GCT ACT GAA AAA GGA AAA ATG AAT AAT 288Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn915 920 925 930TTT TTA GAT AAT AAA AAT GAT ATA AAG ACA AAT TAT AAA GAA ATT ACT 336Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr935 940 945TTT TCT ATG GCA GGC TCA TTT GAA GAT GAA ATA AAA GAT TTA AAA GAA 384Phe Ser Met Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu950 955 960ATT GAT AAG ATG TTT GAT AAA ACC AAT CTA TCA AAT TCT ATT ATC ACC 432Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr965 970 975TAT AAA AAT GTG GAA CCG ACA ACA ATT GGA TTT AAT AAA TCT TTA ACA 480Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr980 985 990GAA GGT AAT ACG ATT AAT TCT GAT GCA ATG GCA CAG TTT AAA GAA CAA 528Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln995 100010051010TTT TTA GAT AGG GAT ATT AAG TTT GAT AGT TAT CTA GAT ACG CAT TTA 576Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu
101510201025ACT GCT CAA CAA GTT TCC AGT AAA GAA AGA GTT ATT TTG AAG GTT ACG624Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr1030 10351040GTT CCG AGT GGG AAA GGT TCT ACT ACT CCA ACA AAA GCA GGT GTC ATT672Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile104510501055TTA AAT AAT AGT GAA TAC AAA ATG CTC ATT GAT AAT GGG TAT ATG GTC720Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val106010651070CAT GTA GAT AAG GTA TCA AAA GTG GTG AAA AAA GGG GTG GAG TGC TTA768His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu1075108010851090CAA ATT GAA GGG ACT TTA AAA AAG AGT CTT GAC TTT AAA AAT GAT ATA816Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile109511001105AAT GCT GAA GCG CAT AGC TGG GGT ATG AAG AAT TAT GAA GAG TGG GCT864Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala111011151120AAA GAT TTA ACC GAT TCG CAA AGG GAA GCT TTA GAT GGG TAT GCT AGG912Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg112511301135CAA GAT TAT AAA GAA ATC AAT AAT TAT TTA AGA AAT CAA GGC GGA AGT960Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser114011451150GGA AAT GAA AAA CTA GAT GCT CAA ATA AAA AAT ATT TCT GAT GCT TTA 1008Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu1155116011651170GGG AAG AAA CCA ATA CCG GAA AAT ATT ACT GTG TAT AGA TGG TGT GGC 1056Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly117511801185ATG CCG GAA TTT GGT TAT CAA ATT AGT GAT CCG TTA CCT TCT TTA AAA 1104Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys119011951200GAT TTT GAA GAA CAA TTT TTA AAT ACA ATC AAA GAA GAC AAA GGA TAT 1152Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr120512101215ATG AGT ACA AGC TTA TCG AGT GAA CGT CTT GCA GCT TTT GGA TCT AGA 1200Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg122012251230AAA ATT ATA TTA CGA TTA CAA GTT CCG AAA GGA AGT ACG GGT GCG TAT 1248Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr1235124012451250TTA AGT GCC ATT GGT GGA TTT GCA AGT GAA AAA GAG ATC CTA CTT GAT 1296Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp125512601265AAA GAT AGT AAA TAT CAT ATT GAT AAA GTA ACA GAG GTA ATT ATT AAA 1344Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys127012751280GGT GTT AAG CGA TAT GTA GTG GAT GCA ACA TTA TTA ACA AAT ATG AAA 1392Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn Met Lys128512901295AAT ATG AAG AAA AAG TTA GCA AGT GTT GTA ACG TGT ACG TTA TTA GCT 1440Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Thr Leu Leu Ala130013051310CCT ATG TTT TTG AAT GGA AAT GTG AAT GCT GTT TAC GCA GAC AGC AAA 1488Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Tyr Ala Asp Ser Lys1315132013251330ACA AAT CAA ATT TCT ACA ACA CAG AAA AAT CAA CAG AAA GAG ATG GAC 1536Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln Lys Glu Met Asp133513401345CGA AAA GGA TTA CTT GGG TAT TAT TTC AAA GGA AAA GAT TTT AGT AAT 1584Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe Ser Asn135013551360CTT ACT ATG TTT GCA CCG ACA CGT GAT AGT ACT CTT ATT TAT GAT CAA 1632Leu Thr Met Phe Ala Pro Thr Arg Asp Ser Thr Leu Ile Tyr Asp Gln136513701375CAA ACA GCA AAT AAA CTA TTA GAT AAA AAA CAA CAA GAA TAT CAG TCT 1680Gln Thr Ala Asn Lys Leu Leu Asp Lys Lys Gln Gln Glu Tyr Gln Ser138013851390ATT CGT TGG ATT GGT TTG ATT CAG AGT AAA GAA ACG GGA GAT TTC ACA 1728Ile Arg Trp Ile Gly Leu Ile Gln Ser Lys Glu Thr Gly Asp Phe Thr1395140014051410TTT AAC TTA TCT GAG GAT GAA CAG GCA ATT ATA GAA ATC AAT GGG AAA 1776Phe Asn Leu Ser Glu Asp Glu Gln Ala Ile Ile Glu Ile Asn Gly Lys141514201425ATT ATT TCT AAT AAA GGG AAA GAA AAG CAA GTT GTC CAT TTA GAA AAA 1824Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val His Leu Glu Lys143014351440GGA AAA TTA GTT CCA ATC AAA ATA GAG TAT CAA TCA GAT ACA AAA TTT 1872Gly Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser Asp Thr Lys Phe144514501455AAT ATT GAC AGT AAA ACA TTT AAA GAA CTT AAA TTA TTT AAA ATA GAT 1920Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu Phe Lys Ile Asp146014651470AGT CAA AAC CAA CCC CAG CAA GTC CAG CAA GAT GAA CTG AGA AAT CCT 1968Ser Gln Asn Gln Pro Gln Gln Val Gln Gln Asp Glu Leu Arg Asn Pro1475148014851490GAA TTT AAC AAG AAA GAA TCA CAG GAA TTC TTA GCG AAA CCA TCG AAA 2016Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Pro Ser Lys149515001505ATA AAT CTT TTC ACT CAA AAA ATG AAA AGG GAA ATT GAT GAA GAC ACG 2064Ile Asn Leu Phe Thr Gln Lys Met Lys Arg Glu Ile Asp Glu Asp Thr151015151520GAT ACG GAT GGG GAC TCT ATT CCT GAC CTT TGG GAA GAA AAT GGG TAT 2112Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn Gly Tyr152515301535ACG ATT CAA AAT AGA ATC GCT GTA AAG TGG GAC GAT TCT CTA GCA AGT 2160Thr Ile Gln Asn Arg Ile Ala Val Lys Trp Asp Asp Ser Leu Ala Ser154015451550AAA GGG TAT ACG AAA TTT GTT TCA AAT CCA CTA GAA AGT CAC ACA GTT 2208Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Glu Ser His Thr Val1555156015651570GGT GAT CCT TAT ACA GAT TAT GAA AAG GCA GCA AGA GAT CTA GAT TTG 2256Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala ArG Asp Leu Asp Leu157515801585TCA AAT GCA AAG GAA ACG TTT AAC CCA TTG GTA GCT GCT TTT CCA AGT 2304Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe Pro Ser159015951600GTG AAT GTT AGT ATG GAA AAG GTG ATA TTA TCA CCA AAT GAA AAT TTA 2352Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu Asn Leu160516101615TCC AAT AGT GTA GAG TCT CAT TCA TCC ACG AAT TGG TCT TAT ACA AAT 2400Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr Thr Asn162016251630ACA GAA GGT GCT TCT GTT GAA GCG GGG ATT GGA CCA AAA GGT ATT TCG 2448Thr Glu Gly Ala Ser Val Glu Ala Gly Ile Gly Pro Lys Gly Ile Ser1635164016451650TTC GGA GTT AGC GTA AAC TAT CAA CAC TCT GAA AGA GTT GCA CAA GAA 2496Phe Gly Val Ser Val Asn Tyr Gln His Ser Glu Thr Val Ala Gln Glu165516601665TGG GGA ACA TCT ACA GGA AAT ACT TCG CAA TTC AAT ACG GCT TCA GCG 2544Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala Ser Ala16701675 1680GGA TAT TTA AAT GCA AAT GTT CGA TAT AAC AAT GTA GGA ACT GGT GCC 2592Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr Gly Ala168516901695ATC TAC GAT GTA AAA CCT ACA ACA AGT TTT GTA TTA AAT AAC GAT ACT 2640Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn Asp Thr170017051710ATC GCA ACT ATT ACG GCG AAA TCT AAT TCT ACA GCC TTA AAT ATA TCT 2688Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Asn Ile Ser1715172017251730CCT GGA GAA AGT TAC CCG AAA AAA GGA CAA AAT GGA ATC GCA ATA ACA 2736Pro Gly Glu Ser Tyr Pro Lys Lys Gly Gln Asn Gly Ile Ala Ile Thr173517401745TCA ATG GAT GAT TTT AAT TCC CAT CCG ATT ACA TTA AAT AAA AAA CAA 2784Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys Lys Gln175017551760GTA GAT AAT CTG CTA AAT AAT AAA CCT ATG ATG TTG GAA ACA AAC CAA 2832Val Asp Asn Leu Leu Asn Asn Lys Pro Met Met Leu Glu Thr Asn Gln176517701775ACA GAT GGT GTT TAT AAG ATA AAA GAT ACA CAT GGA AAT ATA GTA ACT 2880Thr Asp Gly Val Tyr Lys Ile Lys Asp Thr His Gly Asn Ile Val Thr178017851790GGC GGA GAA TGG AAT GGT GTC ATA CAA CAA ATC AAG GCT AAA ACA GCG 2928Gly Gly Glu Trp Asn Gly Val Ile Gln Gln Ile Lys Ala Lys Thr Ala1795180018051810TCT ATT ATT GTG GAT GAT GGG GAA CGT GTA GCA GAA AAA CGT GTA GCG 2976Ser Ile Ile Val Asp Asp Gly Glu Arg Val Ala Glu Lys Arg Val Ala181518201825GCA AAA GAT TAT GAA AAT CCA GAA GAT AAA ACA CCG TCT TTA ACT TTA 3024Ala Lys Asp Tyr Glu Asn Pro Glu Asp Lys Thr Pro Ser Leu Thr Leu183018351840AAA GAT GCC CTG AAG CTT TCA TAT CCA GAT GAA ATA AAA GAA ATA GAG 3072Lys Asp Ala Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu Ile Glu184518501855GGA TTA TTA TAT TAT AAA AAC AAA CCG ATA TAC GAA TCG AGC GTT ATG 3120Gly Leu Leu Tyr Tyr Lys Asn Lys Pro Ile Tyr Glu Ser Ser Val Met186018651870ACT TAC TTA GAT GAA AAT ACA GCA AAA GAA GTG ACC AAA CAA TTA AAT 3168Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Thr Lys Gln Leu Asn1875188018851890GAT ACC ACT GGG AAA TTT AAA GAT GTA AGT CAT TTA TAT GAT GTA AAA 3216Asp Thr Thr Gly Lys Phe Lys Asp Val Ser His Leu Tyr Asp Val Lys
189519001905CTG ACT CCA AAA ATG AAT GTT ACA ATC AAA TTG TCT ATA CTT TAT GAT 3264Leu Thr Pro Lys Met Asn Val Thr Ile Lys Leu Ser Ile Leu Tyr Asp191019151920AAT GCT GAG TCT AAT GAT AAC TCA ATT GGT AAA TGG ACA AAC ACA AAT 3312Ash Ala Glu Ser Asn Asp Asn Ser Ile Gly Lys Trp Thr Asn Thr Asn192519301935ATT GTT TCA GGT GGA AAT AAC GGA AAA AAA CAA TAT TCT TCT AAT AAT 3360Ile Val Ser Gly Gly Asn Asn Gly Lys Lys Gln Tyr Ser Ser Asn Asn194019451950CCG GAT GCT AAT TTG ACA TTA AAT ACA GAT GCT CAA GAA AAA TTA AAT 3408Pro Asp Ala Asn Leu Thr Leu Asn Thr Asp Ala Gln Glu Lys Leu Asn1955196019651970AAA AAT CGT GAC TAT TAT ATA AGT TTA TAT ATG AAG TCA GAA AAA AAC 3456Lys Asn Arg Asp Tyr Tyr Ile Ser Leu Tyr Met Lys Ser Glu Lys Asn197519801985ACA CAA TGT GAG ATT ACT ATA GAT GGG GAG ATT TAT CCG ATC ACT ACA 3504Thr Gln Cys Glu Ile Thr Ile Asp Gly Glu Ile Tyr Pro Ile Thr Thr199019952000AAA ACA GTG AAT GTG AAT AAA GAC AAT TAC AAA AGA TTA GAT ATT ATA 3552Lys Thr Val Asn Val Asn Lys Asp Asn Tyr Lys Arg Leu Asp Ile Ile200520102015GCT CAT AAT ATA AAA AGT AAT CCA ATT TCT TCA CTT GAT ATT AAA ACG 3600Ala His Asn Ile Lys Ser Asn Pro Ile Ser Ser Leu His Ile Lys Thr202020252030AAT GAT GAA ATA ACT TTA TTT TGG GAT GAT ATT TCT ATA ACA GAT GTA 3648Asn Asp Glu Ile Thr Leu Phe Trp Asp Asp Ile Ser Ile Thr Asp Val2035204020452050GCA TCA ATA AAA CCG GAA AAT TTA ACA GAT TCA GAA ATT AAA CAG ATT 3696Ala Ser Ile Lys Pro Glu Asn Leu Thr Asp Ser Glu Ile Lys Gln Ile205520602065TAT AGT AGG TAT GGT ATT AAG TTA GAA GAT GGA ATC CTT ATT GAT AAA 3744Tyr Ser Arg Tyr Gly Ile Lys Leu Glu Asp Gly Ile Leu Ile Asp Lys207020752080AAA GGT GGG ATT CAT TAT GGT GAA TTT ATT AAT GAA GCT AGT TTT AAT 3792Lys Gly Gly Ile His Tyr Gly Glu Phe Ile Asn Glu Ala Ser Phe Asn208520902095ATT GAA CCA TTG CAA AAT TAT GTG ACC AAA TAT GAA GTT ACT TAT AGT 3840Ile Glu Pro Leu Gln Asn Tyr Val Thr Lys Tyr Glu Val Thr Tyr Ser210021052110AGT GAG TTA GGA CCA AAC GTG AGT GAC ACA CTT GAA AGT GAT AAA ATT 3888Ser Glu Leu Gly Pro Asn Val Ser Asp Thr Leu Glu Ser Asp Lys Ile2115212021252130TAC AAG GAT GGG ACA ATT AAA TTT GAT TTT ACC AAA TAT AGT AAA AAT 3936Tyr Lys Asp Gly Thr Ile Lys Phe Asp Phe Thr Lys Tyr Ser Lys Asn213521402145GAA CAA GGA TTA TTT TAT GAC AGT GGA TTA AAT TGG GAC TTT AAA ATT 3984Glu Gln Gly Leu Phe Tyr Asp Ser Gly Leu Asn Trp Asp Phe Lys Ile215021552160AAT GCT ATT ACT TAT GAT GGT AAA GAG ATG AAT GTT TTT CAT AGA TAT 4032Asn Ala Ile Thr Tyr Asp Gly Lys Glu Met Asn Val Phe His Arg Tyr216521702175AAT AAA TAG 4041Asn Lys2180(2)关于SEQ ID NO:23的信息(ⅰ)序列特征(A)长度1346个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:23:Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln1 5 10 15Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu20 25 30Leu Asn Asn Glu Val Ile Lys Ala Glu Gln Leu Asn Ile Asn Ser Gln35 40 45Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu50 55 60Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys65 70 75 80Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn85 90 95Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr100 105 110Phe Ser Met Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu115 120 125Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr130 135 140Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr145 150 155 160Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln165 170 175Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu180 185 190Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr195 200 205Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile210 215 220Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val225 230 235 240His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu245 250 255Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile260 265 270Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala275 280 285Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg290 295 300Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser305 310 315 320Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu325 330 335Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly340 345 350Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys355 360 365Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr370 375 380Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg385 390 395 400Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr405 410 415Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp
420 425 430Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys435 440 445Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn Met Lys450 455 460Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Thr Leu Leu Ala465 470 475 480Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Tyr Ala Asp Ser Lys485 490 495Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln Lys Glu Met Asp500 505 510Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe Ser Asn515 520 525Leu Thr Met Phe Ala Pro Thr Arg Asp Ser Thr Leu Ile Tyr Asp Gln530 535 540Gln Thr Ala Asn Lys Leu Leu Asp Lys Lys Gln Gln Glu Tyr Gln Ser545 550 555 560Ile Arg Trp Ile Gly Leu Ile Gln Ser Lys Glu Thr Gly Asp Phe Thr565 570 575Phe Asn Leu Ser Glu Asp Glu Gln Ala Ile Ile Glu Ile Asn Gly Lys580 585 590Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val His Leu Glu Lys595 600 605Gly Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser Asp Thr Lys Phe610 615 620Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu Phe Lys Ile Asp625 630 635 640Ser Gln Asn Gln Pro Gln Gln Val Gln Gln Asp Glu Leu Arg Asn Pro645 650 655Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Pro Ser Lys660 665 670Ile Asn Leu Phe Thr Gln Lys Met Lys Arg Glu Ile Asp Glu Asp Thr675 680 685Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn Gly Tyr690 695 700Thr Ile Gln Asn Arg Ile Ala Val Lys Trp Asp Asp Ser Leu Ala Ser705 710 715 720Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Glu Ser His Thr Val725 730 735Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg Asp Leu Asp Leu740 745 750Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe Pro Ser755 760 765Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu Asn Leu770 775 780Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr Thr Asn785 790 795 800Thr Glu Gly Ala Ser Val Glu Ala Gly Ile Gly Pro Lys Gly Ile Ser805 810 815Phe Gly Val Ser Val Asn Tyr Gln His Ser Glu Thr Val Ala Gln Glu820 825 830Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala Ser Ala835 840 845Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr Gly Ala850 855 860Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn Asp Thr865 870 875 880Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Asn Ile Ser885 890 895Pro Gly Glu Ser Tyr Pro Lys Lys Gly Gln Asn Gly Ile Ala Ile Thr900 905 910Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys Lys Gln915 920 925Val Asp Asn Leu Leu Asn Asn Lys Pro Met Met Leu Glu Thr Asn Gln930 935 940Thr Asp Gly Val Tyr Lys Ile Lys Asp Thr His Gly Asn Ile Val Thr945 950 955 960Gly Gly Glu Trp Asn Gly Val Ile Gln Gln Ile Lys Ala Lys Thr Ala965 970 975Ser Ile Ile Val Asp Asp Gly Glu Arg Val Ala Glu Lys Arg Val Ala980 985 990Ala Lys Asp Tyr Glu Asn Pro Glu Asp Lys Thr Pro Ser Leu Thr Leu995 10001005Lys Asp Ala Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu Ile Glu101010151020Gly Leu Leu Tyr Tyr Lys Asn Lys Pro Ile Tyr Glu Ser Ser Val Met1025103010351040Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Thr Lys Gln Leu Asn104510501055Asp Thr Thr Gly Lys Phe Lys Asp Val Ser His Leu Tyr Asp Val Lys106010651070Leu Thr Pro Lys Met Asn Val Thr Ile Lys Leu Ser Ile Leu Tyr Asp107510801085Asn Ala Glu Ser Asn Asp Asn Ser Ile Gly Lys Trp Thr Asn Thr Asn109010951100Ile Val Ser Gly Gly Asn Asn Gly Lys Lys Gln Tyr Ser Ser Asn Asn1105111011151120Pro Asp Ala Asn Leu Thr Leu Asn Thr Asp Ala Gln Glu Lys Leu Asn112511301135Lys Asn Arg Asp Tyr Tyr Ile Ser Leu Tyr Met Lys Ser Glu Lys Asn114011451150Thr Gln Cys Glu Ile Thr Ile Asp Gly Glu Ile Tyr Pro Ile Thr Thr115511601165Lys Thr Val Asn Val Asn Lys Asp Asn Tyr Lys Arg Leu Asp Ile Ile117011751180Ala His Asn Ile Lys Ser Asn Pro Ile Ser Ser Leu His Ile Lys Thr1185119011951200Asn Asp Glu Ile Thr Leu Phe Trp Asp Asp Ile Ser Ile Thr Asp Val120512101215Ala Ser Ile Lys Pro Glu Asn Leu Thr Asp Ser Glu Ile Lys Gln Ile122012251230Tyr Ser Arg Tyr Gly Ile Lys Leu Glu Asp Gly Ile Leu Ile Asp Lys123512401245Lys Gly Gly Ile His Tyr Gly Glu Phe Ile Asn Glu Ala Ser Phe Asn125012551260Ile Glu Pro Leu Gln Asn Tyr Val Thr Lys Tyr Glu Val Thr Tyr Ser1265127012751280Ser Glu Leu Gly Pro Asn Val Ser Asp Thr Leu Glu Ser Asp Lys Ile1285129 1295Tyr Lys Asp Gly Thr Ile Lys Phe Asp Phe Thr Lys Tyr Ser Lys Asn130013051310Glu Gln Gly Leu Phe Tyr Asp Ser Gly Leu Asn Trp Asp Phe Lys Ile131513201325Asn Ala Ile Thr Tyr Asp Gly Lys Glu Met Asn Val Phe His Arg Tyr133013351340Asn Lys1345(2)关于SEQ ID NO:24的信息(ⅰ)序列特征(A)长度1399个核苷酸(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅸ)特征(A)名称/键misc_feature(B)位置1..1386(D)其它信息/备注=“来自AB78的编码VIP2A(a)的玉米最佳化DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:24:ATGAAGCGCA TGGAGGGCAA GCTGTTCATG GTGAGCAAGA AGCTCCAGGT GGTGACCAAG60ACCGTGCTGC TGAGCACCGT GTTCAGCATC AGCCTGCTGA ACAACGAGGT GATCAAGGCC 120GAGCAGCTGA ACATCAACAG CCAGAGCAAG TACACCAACC TCCAGAACCT GAAGATCACC 180GACAAGGTGG AGGACTTCAA GGAGGACAAG GAGAAGGCCA AGGAGTGGGG CAAGGAGAAG 240GAGAAGGAGT GGAAGCTTAC CGCCACCGAG AAGGGCAAGA TGAACAACTT CCTGGACAAC 300AAGAACGACA TCAAGACCAA CTACAAGGAG ATCACCTTCA GCATGGCCGG CAGCTTCGAG 360GACGAGATCA AGGACCTGAA GGAGATCGAC AAGATGTTCG ACAAGACCAA CCTGAGCAAC 420AGCATCATCA CCTACAAGAA CGTGGAGCCC ACCACCATCG GCTTCAACAA GAGCCTGACC 480GAGGGCAACA CCATCAACAG CGACGCCATG GCCCAGTTCA AGGAGCAGTT CCTGGACCGC 540GACATCAAGT TCGACAGCTA CCTGGACACC CACCTGACCG CCCAGCAGGT GAGCAGCAAG 600GAGCGCGTGA TCCTGAAGGT GACCGTCCCC AGCGGCAAGG GCAGCACCAC CCCCACCAAG 660GCCGGCGTGA TCCTGAACAA CAGCGAGTAC AAGATGCTGA TCGACAACGG CTACATGGTG 720CACGTGGACA AGGTGAGCAA GGTGGTGAAG AAGGGCGTGG AGTGCCTCCA GATCGAGGGC 780ACCCTGAAGA AGAGTCTAGA CTTCAAGAAC GACATCAACG CCGAGGCCCA CAGCTGGGGC 840ATGAAGAACT ACGAGGAGTG GGCCAAGGAC CTGACCGACA GCCAGCGCGA GGCCCTGGAC 900GGCTACGCCC GCCAGGACTA CAAGGAGATC AACAACTACC TGCGCAACCA GGGCGGCAGC 960GGCAACGAGA AGCTGGACGC CCAGATCAAG AACATCAGCG ACGCCCTGGG CAAGAAGCCC 1020ATCCCCGAGA ACATCACCGT GTACCGCTGG TGCGGCATGC CCGAGTTCGG CTACCAGATC 1080AGCGACCCCC TGCCCAGCCT GAAGGACTTC GAGGAGCAGT TCCTGAACAC CATCAAGGAG 1140GACAAGGGCT ACATGAGCAC CAGCCTGAGC AGCGAGCGCC TGGCCGCCTT CGGCAGCCGC 1200AAGATCATCC TGCGCCTGCA GGTGCCCAAG GGCAGCACCG GCGCCTACCT GAGCGCCATC 1260GGCGGCTTCG CCAGCGAGAA GGAGATCCTG CTGGACAAGG ACAGCAAGTA CCACATCGAC 1320AAGGTGACCG AGGTGATCAT CAAGGGCGTG AAGCGCTACG TGGTGGACGC CACCCTGCTG 1380ACCAACTAGA TCTGAGCTC 1399(2)关于SEQ ID NO:25的信息(ⅰ)序列特征(A)长度19个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型肽(ⅸ)特征(A)名称/键肽(B)位置1..19(D)其它信息/备注=“从细胞向外分泌VIP2的分泌信号肽”(ⅹⅰ)序列描述SEQ ID NO:25:Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly Val1 5 10 15His Cys Leu(2)关于SEQ ID NO:26的信息(ⅰ)序列特征(A)长度2655个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键misc_feathure(B)位置1..2655(D)其它信息/备注=“编码VIP1A(a)的玉米最佳化DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:26:ATGAAGAACA TGAAGAAGAA GCTGGCCAGC GTGGTGACCT GCACCCTGCT GGCCCCCATG60TTCCTGAACG GCAACGTGAA CGCCGTGTAC GCCGACAGCA AGACCAACCA GATCAGCACC 120ACCCAGAAGA ACCAGCAGAA GGAGATGGAC CGCAAGGGCC TGCTGGGCTA CTACTTCAAG 180GGCAAGGACT TCAGCAACCT GACCATGTTC GCCCCCACGC GTGACAGCAC CCTGATCTAC 240GACCAGCAGA CCGCCAACAA GCTGCTGGAC AAGAAGCAGC AGGAGTACCA GAGCATCCGC 300TGGATCGGCC TGATCCAGAG CAAGGAGACC GGCGACTTCA CCTTCAACCT GAGCGAGGAC 360GAGCAGGCCA TCATCGAGAT CAACGGCAAG ATCATCAGCA ACAAGGGCAA GGAGAAGCAG 420GTGGTGCACC TGGAGAAGGG CAAGCTGGTG CCCATCAAGA TCGAGTACCA GAGCGACACC 480AAGTTCAACA TCGACAGCAA GACCTTCAAG GAGCTGAAGC TTTTCAAGAT CGACAGCCAG 540AACCAGCCCC AGCAGGTGCA GCAGGACGAG CTGCGCAACC CCGAGTTCAA CAAGAAGGAG 600AGCCAGGAGT TCCTGGCCAA GCCCAGCAAG ATCAACCTGT TCACCCAGCA GATGAAGCGC 660GAGATCGACG AGGACACCGA CACCGACGGC GACAGCATCC CCGACCTGTG GGAGGAGAAC 720GGCTACACCA TCCAGAACCG CATCGCCGTG AAGTGGGACG ACAGCCTGGC TAGCAAGGGC 780TACACCAAGT TCGTGAGCAA CCCCCTGGAG AGCCACACCG TGGGCGACCC CTACACCGAC 840TACGAGAAGG CCGCCCGCGA CCTGGACCTG AGCAACGCCA AGGAGACCTT CAACCCCCTG 900GTGGCCGCCT TCCCCAGCGT GAACGTGAGC ATGGAGAAGG TGATCCTGAG CCCCAACGAG 960AACCTGAGCA ACAGCGTGGA GAGCCACTCG AGCACCAACT GGAGCTACAC CAACACCGAG 1020GGCGCCAGCG TGGAGGCCGG CATCGGTCCC AAGGGCATCA GCTTCGGCGT GAGCGTGAAC 1080TACCAGCACA GCGAGACCGT GGCCCAGGAG TGGGGCACCA GCACCGGCAA CACCAGCCAG 1140TTCAACACCG CCAGCGCCGG CTACCTGAAC GCCAACGTGC GCTACAACAA CGTGGGCACC 1200GGCGCCATCT ACGACGTGAA GCCCACCACC AGCTTCGTGC TGAACAACGA CACCATCGCC 1260ACCATCACCG CCAAGTCGAA TTCCACCGCC CTGAACATCA GCCCCGGCGA GAGCTACCCC 1320AAGAAGGGCC AGAACGGCAT CGCCATCACC AGCATGGACG ACTTCAACAG CCACCCCATC 1380ACCCTGAACA AGAAGCAGGT GGACAACCTG CTGAACAACA AGCCCATGAT GCTGGAGACC 1440AACCAGACCG ACGGCGTCTA CAAGATCAAG GACACCCACG GCAACATCGT GACGGGCGGC 1500GAGTGGAACG GCGTGATCCA GCAGATCAAG GCCAAGACCG CCAGCATCAT CGTCGACGAC 1560GGCGAGCGCG TGGCCGAGAA GCGCGTGGCC GCCAAGGACT ACGAGAACCC CGAGGACAAG 1620ACCCCCAGCC TGACCCTGAA GGACGCCCTG AAGCTGAGCT ACCCCGACGA GATCAAGGAG 1680ATCGAGGGCT TGCTGTACTA CAAGAACAAG CCCATCTACG AGAGCAGCGT GATGACCTAT 1740CTAGACGAGA ACACCGCCAA GGAGGTGACC AAGCAGCTGA ACGACACCAC CGGCAAGTTC 1800AAGGACGTGA GCCACCTGTA CGACGTGAAG CTGACCCCCA AGATGAACGT GACCATCAAG 1860CTGAGCATCC TGTACGACAA CGCCGAGAGC AACGACAACA GCATCGGCAA GTGGACCAAC 1920ACCAACATCG TGAGCGGCGG CAACAACGGC AAGAAGCAGT ACAGCAGCAA CAACCCCGAC 1980GCCAACCTGA CCCTGAACAC CGACGCCCAG GAGAAGCTGA ACAAGAACCG CGACTACTAC 2040ATCAGCCTGT ACATGAAGAG CGAGAAGAAC ACCCAGTGCG AGATCACCAT CGACGGCGAG 2100ATATACCCCA TCACCACCAA GACCGTGAAC GTGAACAAGG ACAACTACAA GCGCCTGGAC 2160ATCATCGCCC ACAACATCAA GAGCAACCCC ATCAGCAGCC TGCACATCAA GACCAACGAC 2220GAGATCACCC TGTTCTGGGA CGACATATCG ATTACCGACG TCGCCAGCAT CAAGCCCGAG 2280AACCTGACCG ACAGCGAGAT CAAGCAGATA TACAGTCGCT ACGGCATCAA GCTGGAGGAC 2340GGCATCCTGA TCGACAAGAA AGGCGGCATC CACTACGGCG AGTTCATCAA CGAGGCCAGC 2400TTCAACATCG AGCCCCTGCA GAACTACGTG ACCAAGTACG AGGTGACCTA CAGCAGCGAG 2460CTGGGCCCCA ACGTGAGCGA CACCCTGGAG AGCGACAAGA TTTACAAGGA CGGCACCATC 2520AAGTTCGACT TCACCAAGTA CAGCAAGAAC GAGCAGGGCC TGTTCTACGA CAGCGGCCTG 2580AACTGGGACT TCAAGATCAA CGCCATCACC TACGACGGCA AGGAGATGAA CGTGTTCCAC 2640CGCTACAACA AGTAG 2655(2)关于SEQ ID NO:27的信息(ⅰ)序列特征(A)长度1389个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键misc_feathure(B)位置1..1389(D)其它信息/备注=“编码VIP2A(a)的玉米最佳化DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:27:ATGAAGCGCA TGGAGGGCAA GCTGTTCATG GTGAGCAAGA AGCTCCAGGT GGTGACCAAG60ACCGTGCTGC TGAGCACCGT GTTCAGCATC AGCCTGCTGA ACAACGAGGT GATCAAGGCC 120GAGCAGCTGA ACATCAACAG CCAGAGCAAG TACACCAACC TCCAGAACCT GAAGATCACC 180GACAAGGTGG AGGACTTCAA GGAGGACAAG GAGAAGGCCA AGGAGTGGGG CAAGGAGAAG 240GAGAAGGAGT GGAAGCTTAC CGCCACCGAG AAGGGCAAGA TGAACAACTT CCTGGACAAC 300AAGAACGACA TCAAGACCAA CTACAAGGAG ATCACCTTCA GCATAGCCGG CAGCTTCGAG 360GACGAGATCA AGGACCTGAA GGAGATCGAC AAGATGTTCG ACAAGACCAA CCTGAGCAAC 420AGCATCATCA CCTACAAGAA CGTGGAGCCC ACCACCATCG GCTTCAACAA GAGCCTGACC 480GAGGGCAACA CCATCAACAG CGACGCCATG GCCCAGTTCA AGGAGCAGTT CCTGGACCGC 540GACATCAAGT TCGACAGCTA CCTGGACACC CACCTGACCG CCCAGCAGGT GAGCAGCAAG 600GAGCGCGTGA TCCTGAAGGT GACCGTCCCC AGCGGCAAGG GCAGCACCAC CCCCACCAAG 660GCCGGCGTGA TCCTGAACAA CAGCGAGTAC AAGATGCTGA TCGACAACGG CTACATGGTG 720CACGTGGACA AGGTGAGCAA GGTGGTGAAG AAGGGCGTGG AGTGCCTCCA GATCGAGGGC 780ACCCTGAAGA AGAGTCTAGA CTTCAAGAAC GACATCAACG CCGAGGCCCA CAGCTGGGGC 840ATGAAGAACT ACGAGGAGTG GGCCAAGGAC CTGACCGACA GCCAGCGCGA GGCCCTGGAC 900GGCTACGCCC GCCAGGACTA CAAGGAGATC AACAACTACC TGCGCAACCA GGGCGGCAGC 960GGCAACGAGA AGCTGGACGC CCAGATCAAG AACATCAGCG ACGCCCTGGG CAAGAAGCCC 1020ATCCCCGAGA ACATCACCGT GTACCGCTGG TGCGGCATGC CCGAGTTCGG CTACCAGATC 1080AGCGACCCCC TGCCCAGCCT GAAGGACTTC GAGGAGCAGT TCCTGAACAC CATCAAGGAG 1140GACAAGGGCT ACATGAGCAC CAGCCTGAGC AGCGAGCGCC TGGCCGCCTT CGGCAGCCGC 1200AAGATCATCC TGCGCCTGCA GGTGCCCAAG GGCAGCACTG GTGCCTACCT GAGCGCCATC 1260GGCGGCTTCG CCAGCGAGAA GGAGATCCTG CTGGATAAGG ACAGCAAGTA CCACATCGAC 1320AAGGTGACCG AGGTGATCAT CAAGGGCGTG AAGCGCTACG TGGTGGACGC CACCCTGCTG 1380ACCAACTAG 1389(2)关于SEQ ID NO:28的信息(ⅰ)序列特征(A)长度2378个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置9..2375(D)其它信息/备注=“来自AB88的编码VIP3A(a)蛋白的天然DNA序列，包含于pCIB7104中。”(ⅹⅰ)序列描述SEQ ID NO:28:AGATGAAC ATG AAC AAG AAT AAT ACT AAA TTA AGC ACA AGA GCC TTA CCA50Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro1 5 10AGT TTT ATT GAT TAT TTT AAT GGC ATT TAT GGA TTT GCC ACT GGT ATC 98Ser Phe Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile15 20 25 30AAA GAC ATT ATG AAC ATG ATT TTT AAA ACG GAT AGA GGT GGT GAT CTA146Lys Asp Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu35 40 45ACC CTA GAC GAA ATT TTA AAG AAT CAG CAG TTA CTA AAT GAT ATT TCT194Thr Leu Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser50 55 60GGT AAA TTG GAT GGG GTG AAT GGA AGC TTA AAT GAT CTT ATC GCA CAG242Gly Lys Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln65 70 75GGA AAC TTA AAT ACA GAA TTA TCT AAG GAA ATA TTA AAA ATT GCA AAT290Gly Asn Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn80 85 90GAA CAA AAT CAA GTT TTA AAT GAT GTT AAT AAC AAA CTC GAT GCG ATA338Glu Gln Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile95 100 105 110AAT ACG ATG CTT CGG GTA TAT CTA CCT AAA ATT ACC TCT ATG TTG AGT386Asn Thr Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser115 120 125GAT GTA ATG AAA CAA AAT TAT GCG CTA AGT CTG CAA ATA GAA TAC TTA434Asp Val Met Lys GLn Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu130 135 140AGT AAA CAA TTG CAA GAG ATT TCT GAT AAG TTG GAT ATT ATT AAT GTA482Ser Lys Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val145 150 155AAT GTA CTT ATT AAC TCT ACA CTT ACT GAA ATT ACA CCT GCG TAT CAA530Asn Val Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln160 165 170AGG ATT AAA TAT GTG AAC GAA AAA TTT GAG GAA TTA ACT TTT GCT ACA578Arg Ile Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr175 180 185 190GAA ACT AGT TCA AAA GTA AAA AAG GAT GGC TCT CCT GCA GAT ATT CTT626Glu Thr Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu195 200 205GAT GAG TTA ACT GAG TTA ACT GAA CTA GCG AAA AGT GTA ACA AAA AAT674Asp Glu Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn210 215 220GAT GTG GAT GGT TTT GAA TTT TAC CTT AAT ACA TTC CAC GAT GTA ATG722Asp Val Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met225 230 235GTA GGA AAT AAT TTA TTC GGG CGT TCA GCT TTA AAA ACT GCA TCG GAA770Val Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu240 245 250TTA ATT ACT AAA GAA AAT GTG AAA ACA AGT GGC AGT GAG GTC GGA AAT818Leu Ile Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn255 260 265 270GTT TAT AAC TTC TTA ATT GTA TTA ACA GCT CTG CAA GCC CAA GCT TTT866Val Tyr Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Gln Ala Phe275 280 285CTT ACT TTA ACA ACA TGC CGA AAA TTA TTA GGC TTA GCA GAT ATT GAT914Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp
290 295 300TAT ACT TCT ATT ATG AAT GAA CAT TTA AAT AAG GAA AAA GAG GAA TTT962Tyr Thr Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe305 310 315AGA GTA AAC ATC CTC CCT ACA CTT TCT AAT ACT TTT TCT AAT CCT AAT 1010Arg Val Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn320 325 330TAT GCA AAA GTT AAA GGA AGT GAT GAA GAT GCA AAG ATG ATT GTG GAA 1058Tyr Ala Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu335 340 345 350GCT AAA CCA GGA CAT GCA TTG ATT GGG TTT GAA ATT AGT AAT GAT TCA 1106Ala Lys Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser355 360 365ATT ACA GTA TTA AAA GTA TAT GAG GCT AAG CTA AAA CAA AAT TAT CAA 1154Ile Thr Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln370 375 380GTC GAT AAG GAT TCC TTA TCG GAA GTT ATT TAT GGT GAT ATG GAT AAA 1202Val Asp Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys385 390 395TTA TTG TGC CCA GAT CAA TCT GAA CAA ATC TAT TAT ACA AAT AAC ATA 1250Leu Leu Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile400 405 410GTA TTT CCA AAT GAA TAT GTA ATT ACT AAA ATT GAT TTC ACT AAA AAA 1298Val Phe Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys415 420 425 430ATG AAA ACT TTA AGA TAT GAG GTA ACA GCG AAT TTT TAT GAT TCT TCT 1346Met Lys Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser435 440 445ACA GGA GAA ATT GAC TTA AAT AAG AAA AAA GTA GAA TCA AGT GAA GCG 1394Thr Gly Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala450 455 460GAG TAT AGA ACG TTA AGT GCT AAT GAT GAT GGG GTG TAT ATG CCG TTA 1442Glu Tyr Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu465 470 475GGT GTC ATC AGT GAA ACA TTT TTG ACT CCG ATT AAT GGG TTT GGC CTC 1490Gly Val Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu480 485 490CAA GCT GAT GAA AAT TCA ACA TTA ATT ACT TTA ACA TGT AAA TCA TAT 1538Gln Ala Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr495 500 505 510TTA AGA GAA CTA CTG CTA GCA ACA GAC TTA AGC AAT AAA GAA ACT AAA 1586Leu Arg Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys515 520 525TTG ATC GTC CCG CCA AGT GGT TTT ATT AGC AAT ATT GTA GAG AAC GGG 1634Leu Ile Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly530 535 540TCC ATA GAA GAG GAC AAT TTA GAG CCG TGG AAA GCA AAT AAT AAG AAT 1682Ser Ile Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn545 550 555GCG TAT GTA GAT CAT ACA GGC GGA GTG AAT GGA ACT AAA GCT TTA TAT 1730Ala Tyr Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr560 565 570GTT CAT AAG GAC GGA GGA ATT TCA CAA TTT ATT GGA GAT AAG TTA AAA 1778Val His Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys575 580 585 590CCG AAA ACT GAG TAT GTA ATC CAA TAT ACT GTT AAA GGA AAA CCT TCT 1826Pro Lys Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser595 600 605ATT CAT TTA AAA GAT GAA AAT ACT GGA TAT ATT CAT TAT GAA GAT ACA 1874Ile His Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr610 615 620AAT AAT AAT TTA GAA GAT TAT CAA ACT ATT AAT AAA CGT TTT ACT ACA 1922Asn Asn Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr625 630 635GGA ACT GAT TTA AAG GGA GTG TAT TTA ATT TTA AAA AGT CAA AAT GGA 1970Gly Thr Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly640 645 650GAT GAA GCT TGG GGA GAT AAC TTT ATT ATT TTG GAA ATT AGT CCT TCT 2018Asp Glu Ala Trp Gly Asp Ash Phe Ile Ile Leu Glu Ile Ser Pro Ser655 660 665 670GAA AAG TTA TTA AGT CCA GAA TTA ATT AAT ACA AAT AAT TGG ACG AGT 2066Glu Lys Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser675 680 685ACG GGA TCA ACT AAT ATT AGC GGT AAT ACA CTC ACT CTT TAT CAG GGA 2114Thr Gly Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly690 695 700GGA CGA GGG ATT CTA AAA CAA AAC CTT CAA TTA GAT AGT TTT TCA ACT 2162Gly Arg Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr705 710 715TAT AGA GTG TAT TTT TCT GTG TCC GGA GAT GCT AAT GTA AGG ATT AGA 2210Tyr Arg Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg720 725 730AAT TCT AGG GAA GTG TTA TTT GAA AAA AGA TAT ATG AGC GGT GCT AAA 2258Asn Ser Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys735 740 745 750GAT GTT TCT GAA ATG TTC ACT ACA AAA TTT GAG AAA GAT AAC TTT TAT 2306Asp Val Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr755 760 765ATA GAG CTT TCT CAA GGG AAT AAT TTA TAT GGT GGT CCT ATT GTA CAT 2354Ile Glu Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His770 775 780TTT TAC GAT GTC TCT ATT AAG TAA 2378Phe Tyr Asp Val Ser Ile Lys785(2)关于SEQ ID NO:29的信息(ⅰ)序列特征(A)长度789个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:29:Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe1 5 10 15Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp20 25 30Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu35 40 45Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys50 55 60Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn65 70 75 80Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln85 90 95Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr100 105 110Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val115 120 125Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys130 135 140Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val145 150 155 160Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile165 170 175Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr180 185 190Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu Asp Glu195 200 205Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val210 215 220Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly225 230 235 240Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile245 250 255Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr260 265 270Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Gln Ala Phe Leu Thr275 280 285Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr290 295 300Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val305 310 315 320Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala325 330 335Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys340 345 350Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr355 360 365Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp370 375 380Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu385 390 395 400Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe405 410 415Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys420 425 430Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly
435 440 445Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr450 455 460Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val465 470 475 480Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala485 490 495Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg500 505 510Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile515 520 525Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Ser Ile530 535 540Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr545 550 555 560Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His565 570 575Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys580 585 590Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His595 600 605Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn610 615 620Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr Gly Thr625 630 635 640Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu645 650 655Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys660 665 670Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly675 680 685Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg690 695 700Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg705 710 715 720Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser725 730 735Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val740 745 750Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr Ile Glu755 760 765Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His Phe Tyr770 775 780Asp Val Ser Ile Lys785(2)关于SEQ ID NO:30的信息(ⅰ)序列特征(A)长度2403个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键misc_feature(B)位置11..2389(D)其它信息/备注=“编码VIP3A(a)的玉米最佳化DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:30:GGATCCACCA ATGAACATGA ACAAGAACAA CACCAAGCTG AGCACCCGCG CCCTGCCGAG 60CTTCATCGAC TACTTCAACG GCATCTACGG CTTCGCCACC GGCATCAAGG ACATCATGAA120CATGATCTTC AAGACCGACA CCGGCGGCGA CCTGACCCTG GACGAGATCC TGAAGAACCA180GCAGCTGCTG AACGACATCA GCGGCAAGCT GGACGGCGTG AACGGCAGCC TGAACGACCT240GATCGCCCAG GGCAACCTGA ACACCGAGCT GAGCAAGGAG ATCCTTAAGA TCGCCAACGA300GCAGAACCAG GTGCTGAACG ACGTGAACAA CAAGCTGGAC GCCATCAACA CCATGCTGCG360CGTGTACCTG CCGAAGATCA CCAGCATGCT GAGCGACGTG ATGAAGCAGA ACTACGCCCT420GAGCCHGCAG ATCGAGTACC TGAGCAAGCA GCTGCAGGAG ATCAGCGACA AGCTGGACAT480CATCAACGTG AACGTCCTGA TCAACAGCAC CCTGACCGAG ATCACCCCGG CCTACCAGCG540CATCAAGTAC GTGAACGAGA AGTTCGAAGA GCTGACCTTC GCCACCGAGA CCAGCAGCAA 600GGTGAAGAAG GACGGCAGCC CGGCCGACAT CCTGGACGAG CTGACCGAGC TGACCGAGCT 660GGCCAAGAGC GTGACCAAGA ACGACGTGGA CGGCTTCGAG TTCTACCTGA ACACCTTCCA 720CGACGTGATG GTGGGCAACA ACCTGTTCGG CCGCAGCGCC CTGAAGACCG CCAGCGAGCT 780GATCACCAAG GAGAACGTGA AGACCAGCGG CAGCGAGGTG GGCAACGTGT ACAACTTCCT 840GATCGTGCTG ACCGCCCTGC AGGCCCAGGC CTTCCTGACC CTGACCACCT GTCGCAAGCT 900GCTGGGCCTG GCCGACATCG ACTACACCAG CATCATGAAC GAGCACTTGA ACAAGGAGAA 960GGAGGAGTTC CGCGTGAACA TCCTGCCGAC CCTGAGCAAC ACCTTCAGCA ACCCGAACTA 1020CGCCAAGGTG AAGGGCAGCG ACGAGGACGC CAAGATGATC GTGGAGGCTA AGCCGGGCCA 1080CGCGTTGATC GGCTTCGAGA TCAGCAACGA CAGCATCACC GTGCTGAAGG TGTACGAGGC 1140CAAGCTGAAG CAGAACTACC AGGTGGACAA GGACAGCTTG AGCGAGGTGA TCTACGGCGA 1200CATGGACAAG CTGCTGTGTC CGGACCAGAG CGAGCAAATC TACTACACCA ACAACATCGT 1260GTTCCCGAAC GAGTACGTGA TCACCAAGAT CGACTTCACC AAGAAGATGA AGACCCTGCG 1320CTACGAGGTG ACCGCCAACT TCTACGACAG CAGCACCGGC GAGATCGACC TGAACAAGAA 1380GAAGGTGGAG AGCAGCGAGG CCGAGTACCG CACCCTGAGC GCGAACGACG ACGGCGTCTA 1440CATGCCACTG GGCGTGATCA GCGAGACCTT CCTGACCCCG ATCAACGGCT TTGGCCTGCA 1500GGCCGACGAG AACAGCCGCC TGATCACCCT GACCTGTAAG AGCTACCTGC GCGAGCTGCT 1560GCTAGCCACC GACCTGAGCA ACAAGGAGAC CAAGCTGATC GTGCCACCGA GCGGCTTCAT 1620CAGCAACATC GTGGAGAACG GCAGCATCGA GGAGGACAAC CTGGAGCCGT GGAAGGCCAA 1680CAACAAGAAC GCCTACGTGG ACCACACCGG CGGCGTGAAC GGCACCAAGG CCCTGTACGT 1740GCACAAGGAC GGCGGCATCA GCCAGTTCAT CGGCGACAAG CTGAAGCCGA AGACCGAGTA 1800CGTGATCCAG TACACCGTGA AGGGCAAGCC ATCGATTCAC CTGAAGGACG AGAACACCGG 1860CTACATCCAC TACGAGGACA CCAACAACAA CCTGGAGGAC TACCAGACCA TCAACAAGCG 1920CTTCACCACC GGCACCGACC TGAAGGGCGT GTACCTGATC CTGAAGAGCC AGAACGGCGA 1980CGAGGCCTGG GGCGACAACT TCATCATCCT GGAGATCAGC CCGAGCGAGA AGCTGCTGAG 2040CCCGGAGCTG ATCAACACCA ACAACTGGAC CAGCACCGGC AGCACCAACA TCAGCGGCAA 2100CACCCTGACC CTGTACCAGG GCGGCCGCGG CATCCTGAAG CAGAACCTGC AGCTGGACAG 2160CTTCAGCACC TACCGCGTGT ACTTCAGCGT GAGCGGCGAC GCCAACGTGC GCATCCGCAA 2220CAGCCGCGAG GTGCTGTTCG AGAAGAGGTA CATGAGCGGC GCCAAGGACG TGAGCGAGAT 2280GTTCACCACC AAGTTCGAGA AGGACAACTT CTACATCGAG CTGAGCCAGG GCAACAACCT 2340GTACGGCGGC CCGATCGTGC ACTTCTACGA CGTGAGCATC AAGTTAACGT AGAGCTCAGA 2400TCT2403(2)关于SEQ ID NO:31的信息(ⅰ)序列特征(A)长度2612个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置118..2484(D)其它信息/备注=“来自AB424的编码VIP3A(b)的天然DNA序列。”(ⅹⅰ)序列描述SEQ ID NO:31:ATTGAAATTG ATAAAAAGTT ATGAGTGTTT AATAATCAGT AATTACCAAT AAAGAATTAA 60GAATACAAGT TTACAAGAAA TAAGTGTTAC AAAAAATAGC TGAAAAGGAA GATGAAC 117ATG AAC AAG AAT AAT ACT AAA TTA AGC ACA AGA GCC TTA CCA AGT TTT165Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe790 795 800 805ATT GAT TAT TTC AAT GGC ATT TAT GGA TTT GCC ACT GGT ATC AAA GAC213Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp810 815 820ATT ATG AAC ATG ATT TTT AAA ACG GAT ACA GGT GGT GAT CTA ACC CTA261Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu825 830 835GAC GAA ATT TTA AAG AAT CAG CAG CTA CTA AAT GAT ATT TCT GGT AAA309Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys840 845 850TTG GAT GGG GTG AAT GGA AGC TTA AAT GAT CTT ATC GCA CAG GGA AAC357Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn
855 860 865TTA AAT ACA GAA TTA TCT AAG GAA ATA TTA AAA ATT GCA AAT GAA CAA405Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln870 875 880 885AAT CAA GTT TTA AAT GAT GTT AAT AAC AAA CTC GAT GCG ATA AAT ACG453Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr890 895 900ATG CTT CGG GTA TAT CTA CCT AAA ATT ACC TCT ATG TTG AGT GAT GTA501Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val905 910 915ATG AAA CAA AAT TAT GCG CTA AGT CTG CAA ATA GAA TAC TTA AGT AAA549Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys920 925 930CAA TTG CAA GAG ATT TCT GAT AAG TTG GAT ATT ATT AAT GTA AAT GTA597Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val935 940 945CTT ATT AAC TCT ACA CTT ACT GAA ATT ACA CCT GCG TAT CAA AGG ATT645Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile950 955 960 965AAA TAT GTG AAC GAA AAA TTT GAG GAA TTA ACT TTT GCT ACA GAA ACT693Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr970 975 980AGT TCA AAA GTA AAA AAG GAT GGC TCT CCT GCA GAT ATT CGT GAT GAG741Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Arg Asp Glu985 990 995TTA ACT GAG TTA ACT GAA CTA GCG AAA AGT GTA ACA AAA AAT GAT GTG789Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val100010051010GAT GGT TTT GAA TTT TAC CTT AAT ACA TTC CAC GAT GTA ATG GTA GGA837Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly101510201025AAT AAT TTA TTC GGG CGT TCA GCT TTA AAA ACT GCA TCG GAA TTA ATT885Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile1030103510401045ACT AAA GAA AAT GTG AAA ACA AGT GGC AGT GAG GTC GGA AAT GTT TAT933Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr105010551060AAC TTC CTA ATT GTA TTA ACA GCT CTG CAA GCA AAA GCT TTT CTT ACT981Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Lys Ala Phe Leu Thr106510701075TTA ACA CCA TGC CGA AAA TTA TTA GGC TTA GCA GAT ATT GAT TAT ACT 1029Leu Thr Pro Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr108010851090TCT ATT ATG AAT GAA CAT TTA AAT AAG GAA AAA GAG GAA TTT AGA GTA 1077Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val109511001105AAC ATC CTC CCT ACA CTT TCT AAT ACT TTT TCT AAT CCT AAT TAT GCA 1125Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala1110111511201125AAA GTT AAA GGA AGT GAT GAA GAT GCA AAG ATG ATT GTG GAA GCT AAA 1173Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys113011351140CCA GGA CAT GCA TTG ATT GGG TTT GAA ATT AGT AAT GAT TCA ATT ACA 1221Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr114511501155GTA TTA AAA GTA TAT GAG GCT AAG CTA AAA CAA AAT TAT CAA GTC GAT 1269Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp116011651170AAG GAT TCC TTA TCG GAA GTT ATT TAT GGC GAT ATG GAT AAA TTA TTG 1317Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu117511801185TGC CCA GAT CAA TCT GGA CAA ATC TAT TAT ACA AAT AAC ATA GTA TTT 1365Cys Pro Asp Gln Ser Gly Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe1190119512001205CCA AAT GAA TAT GTA ATT ACT AAA ATT GAT TTC ACT AAA AAA ATG AAA 1413Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys121012151220ACT TTA AGA TAT GAG GTA ACA GCG AAT TTT TAT GAT TCT TCT ACA GGA 1461Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly122512301235GAA ATT GAC TTA AAT AAG AAA AAA GTA GAA TCA AGT GAA GCG GAG TAT 1509Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr124012451250AGA ACG TTA AGT GCT AAT GAT GAT GGG GTG TAT ATG CCG TTA GGT GTC 1557Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val125512601265ATC AGT GAA ACA TTT TTG ACT CCG ATT AAT GGG TTT GGC CTC CAA GCT 1605Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala1270127512801285GAT GAA AAT TCA AGA TTA ATT ACT TTA ACA TGT AAA TCA TAT TTA AGA 1653Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg129012951300GAA CTA CTG CTA GCA ACA GAC TTA AGC AAT AAA GAA ACT AAA TTG ATC 1701Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile130513101315GTC CCG CCA AGT GGT TTT ATT AGC AAT ATT GTA GAG AAC GGG TCC ATA 1749Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Ser Ile132013251330GAA GAG GAC AAT TTA GAG CCG TGG AAA GCA AAT AAT AAG AAT GCG TAT 1797Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr133513401345GTA GAT CAT ACA GGC GGA GTG AAT GGA ACT AAA GCT TTA TAT GTT CAT 1845Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His1350135513601365AAG GAC GGA GGA ATT TCA CAA TTT ATT GGA GAT AAG TTA AAA CCG AAA 1893Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys137013751380ACT GAG TAT GTA ATC CAA TAT ACT GTT AAA GGA AAA CCT TCT ATT CAT 1941Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His138513901395TTA AAA GAT GAA AAT ACT GGA TAT ATP CAT TAT GAA GAT ACA AAT AAT 1989Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn140014051410AAT TTA GAA GAT TAT CAA ACT ATT AAT AAA CGT TTT ACT ACA GGA ACT 2037Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr Gly Thr141514201425GAT TTA AAG GGA GTG TAT TTA ATT TTA AAA AGT CAA AAT GGA GAT GAA 2085Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu1430143514401445GCT TGG GGA GAT AAC TTT ATT ATT TTG GAA ATT AGT CCT TCT GAA AAG 2133Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys145014551460TTA TTA AGT CCA GAA TTA ATT AAT ACA AAT AAT TGG ACG AGT ACG GGA 2181Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly146514701475TCA ACT AAT ATT AGC GGT AAT ACA CTC ACT CTT TAT CAG GGA GGA CGA 2229Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg148014851490GGG ATT CTA AAA CAA AAC CTT CAA TTA GAT AGT TTT TCA ACT TAT AGA 2277Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg149515001505GTG TAT TTC TCT GTG TCC GGA GAT GCT AAT GTA AGG ATT AGA AAT TCT 2325Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser1510151515201525AGG GAA GTG TTA TTT GAA AAA AGA TAT ATG AGC GGT GCT AAA GAT GTT 2373Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val
153015351540TCT GAA ATG TTC ACT ACA AAA TTT GAG AAA GAT AAC TTC TAT ATA GAG 2421Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr Ile Glu154515501555CTT TCT CAA GGG AAT AAT TTA TAT GGT GGT CCT ATT GTA CAT TTT TAC 2469Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His Phe Tyr156015651570GAT GTC TCT ATT AAG TAAGATCGGG ATCTAATATT AACAGGTTTT AGAAGCTAAT 2524Asp Val Ser Ile Lys1575TCTTGTATAA TGTCCTTGAT TATGGAAAAA CACAATTTTG TTTGCTAAGA TGTATATATA2584GCTCACTCAT TAAAAGGCAA TCAAGCTT 2612(2)关于SEQ ID NO:32的信息(ⅰ)序列特征(A)长度789个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:32:Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe1 5 10 15Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp20 25 30Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu35 40 45Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys50 55 60Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn65 70 75 80Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln85 90 95Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr100 105 110Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val115 120 125Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys130 135 140Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val145 150 155 160Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile165 170 175Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr180 185 190Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Arg Asp Glu195 200 205Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val210 215 220Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe Mis Asp Val Met Val Gly225 230 235 240Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile245 250 255Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr260 265 270Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Lys Ala Phe Leu Thr275 280 285Leu Thr Pro Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr290 295 300Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val305 310 315 320Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala325 330 335Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys340 345 350Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr355 360 365Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp370 375 380Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu385 390 395 400Cys Pro Asp Gln Ser Gly Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe405 410 415Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys420 425 430Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly435 440 445Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr450 455 460Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val465 470 475 480Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala485 490 495Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg500 505 510Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile515 520 525Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Ser Ile530 535 540Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr545 550 555 560Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His565 570 575Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys580 585 590Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His595 600 605Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn610 615 620Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr Gly Thr625 630 635 640Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu645 650 655Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys660 665 670Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly675 680 685Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg690 695 700Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg705 710 715 720Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser725 730 735Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val740 745 750Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr Ile Glu755 760 765Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His Phe Tyr770 775 780Asp Val Ser Ile Lys785(2)关于SEQ ID NO:33的信息(ⅰ)序列特征(A)长度30个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于制备pCIB5526的正向引物”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:33:GGATCCACCA TGAAGACCAA CCAGATCAGC 30(2)关于SEQ ID NO:34的信息(ⅰ)序列特征(A)长度15个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于制备pCIB5526的反向引物”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:34:AAGCTTCAGC TCCTT15(2)关于SEQ ID NO:35的信息(ⅰ)序列特征(A)长度2576个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置9..2564(D)其它信息/备注=“编码VIP1A(a)和切出的芽孢杆菌属分泌信号的玉米最佳化DNA序列，包含在pCIB5526中。”(ⅹⅰ)序列描述SEQ ID NO:35:GATCCACC ATG AAG ACC AAC CAG ATC AGC ACC ACC CAG AAG AAC CAG CAG50Met Lys Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln825 830 835AAG GAG ATG GAC CGC AAG GGC CTG CTG GGC TAC TAC TTC AAG GGC AAG 98Lys Glu Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys840 845 850GAC TTC AGC AAC CTG ACC ATG TTC GCC CCC ACG CGT GAC AGC ACC CTG146Asp Phe Ser Asn Leu Thr Met Phe Ala Pro Thr Arg Asp Ser Thr Leu855 860 865ATC TAC GAC CAG CAG ACC GCC AAC AAG CTG CTG GAC AAG AAG CAG CAG194Ile Tyr Asp Gln Gln Thr Ala Asn Lys Leu Leu Asp Lys Lys Gln Gln870 875 880GAG TAC CAG AGC ATC CGC TGG ATC GGC CTG ATC CAG AGC AAG GAG ACC242Glu Tyr Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln Ser Lys Glu Thr885 890 895GGC GAC TTC ACC TTC AAC CTG AGC GAG GAC GAG CAG GCC ATC ATC GAG290Gly Asp Phe Thr Phe Asn Leu Ser Glu Asp Glu Gln Ala Ile Ile Glu900 905 910 915ATC AAC GGC AAG ATC ATC AGC AAC AAG GGC AAG GAG AAG CAG GTG GTG 338Ile Asn Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val920 925 930CAC CTG GAG AAG GGC AAG CTG GTG CCC ATC AAG ATC GAG TAC CAG AGC 386His Leu Glu Lys Gly Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser935 940 945GAC ACC AAG TTC AAC ATC GAC AGC AAG ACC TTC AAG GAG CTG AAG CTT 434Asp Thr Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu950 955 960TTC AAG ATC GAC AGC CAG AAC CAG CCC CAG CAG GTG CAG CAG GAC GAG 482Phe Lys Ile Asp Ser Gln Asn Gln Pro Gln Gln Val Gln Gln Asp Glu965 970 975CTG CGC AAC CCC GAG TTC AAC AAG AAG GAG AGC CAG GAG TTC CTG GCC 530Leu Arg Asn Pro Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala980 9 990 995AAG CCC AGC AAG ATC AAC CTG TTC ACC CAG CAG ATG AAG CGC GAG ATC 578Lys Pro Ser Lys Ile Asn Leu Phe Thr Gln Gln Met Lys Arg Glu Ile100010051010GAC GAG GAC ACC GAC ACC GAC GGC GAC AGC ATC CCC GAC CTG TGG GAG 626Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu101510201025GAG AAC GGC TAC ACC ATC CAG AAC CGC ATC GCC GTG AAG TGG GAC GAC 674Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile Ala Val Lys Trp Asp Asp103010351040AGC CTG GCT AGC AAG GGC TAC ACC AAG TTC GTG AGC AAC CCC CTG GAG 722Ser Leu Ala Ser Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Glu104510501055AGC CAC ACC GTG GGC GAC CCC TAC ACC GAC TAC GAG AAG GCC GCC CGC 770Ser His Thr Val Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg1060106510701075GAC CTG GAC CTG AGC AAC GCC AAG GAG ACC TTC AAC CCC CTG GTG GCC 818Asp Leu Asp Leu Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala108010851090GCC TTC CCC AGC GTG AAC GTG AGC ATG GAG AAG GTG ATC CTG AGC CCC 866Ala Phe Pro Ser Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro109511001105AAC GAG AAC CTG AGC AAC AGC GTG GAG AGC CAC TCG AGC ACC AAC TGG 914Asn Glu Asn Leu Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp111011151120AGC TAC ACC AAC ACC CAG GGC GCC AGC GTG GAG GCC GGC ATC GGT CCC 962Ser Tyr Thr Asn Thr Glu Gly Ala Ser Val Glu Ala Gly Ile Gly Pro112511301135AAG GGC ATC AGC TTC GGC GTG AGC GTG AAC TAC CAG CAC AGC GAG ACC 1010Lys Gly Ile Ser Phe Gly Val Ser Val Asn Tyr Gln His Ser Glu Thr1140114511501155GTG GCC CAG GAG TGG GGC ACC AGC ACC GGC AAC ACC AGC CAG TTC AAC 1058Val Ala Gln Glu Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn116011651170ACC GCC AGC GCC GGC TAC CTG AAC GCC AAC GTG CGC TAC AAC AAC GTG 1106Thr Ala Ser Ala Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val117511801185GGC ACC GGC GCC ATC TAC GAC GTG AAG CCC ACC ACC AGC TTC GTG CTG 1154Gly Thr Gly Ala Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu119011951200AAC AAC GAC ACC ATC GCC ACC ATC ACC GCC AAG TCG AAT TCC ACC GCC 1202Asn Asn Asp Thr Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala120512101215CTG AAC ATC AGC CCC GGC GAG AGC TAC CCC AAG AAG GGC CAG AAC GGC 1250Leu Asn Ile Ser Pro Gly Glu Ser Tyr Pro Lys Lys Gly Gln Asn Gly1220122512301235ATC GCC ATC ACC AGC ATG GAC GAC TTC AAC AGC CAC CCC ATC ACC CTG 1298Ile Ala Ile Thr Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu124012451250AAC AAG AAG CAG GTG GAC AAC CTG CTG AAC AAC AAG CCC ATG ATG CTG 1346Asn Lys Lys Gln Val Asp Asn Leu Leu Asn Asn Lys Pro Met Met Leu125512601265GAG ACC AAC CAG ACC GAC GGC GTC TAC AAG ATC AAG GAC ACC GAC GGC 1394Glu Thr Asn Gln Thr Asp Gly Val Tyr Lys Ile Lys Asp Thr His Gly127012751280AAC ATC GTG ACG GGC GGC GAG TGG AAC GGC GTG ATC CAG CAG ATC AAG 1442Asn Ile Val Thr Gly Gly Glu Trp Asn Gly Val Ile Gln Gln Ile Lys128512901295GCC AAG ACC GCC AGC ATC ATC GTC GAC GAC GGC GAG CGC GTG GCC GAG 1490Ala Lys Thr Ala Ser Ile Ile Val Asp Asp Gly Glu Arg Val Ala Glu1300130513101315AAG CGC GTG GCC GCC AAG GAC TAC GAG AAC CCC GAG GAC AAG ACC CCC 1538Lys Arg Val Ala Ala Lys Asp Tyr Glu Asn Pro Glu Asp Lys Thr Pro132013251330AGC CTG ACC CTG AAG GAC GCC CTG AAG CTG AGC TAC CCC GAC GAG ATC 1586Ser Leu Thr Leu Lys Asp Ala Leu Lys Leu Ser Tyr Pro Asp Glu Ile133513401345AAG GAG ATC GAG GGC TTG CTG TAC TAC AAG AAC AAG CCC ATC TAC GAG 1634Lys Glu Ile Glu Gly Leu Leu Tyr Tyr Lys Asn Lys Pro Ile Tyr Glu135013551360AGC AGC GTG ATG ACC TAT CTA GAC GAG AAC ACC GCC AAG GAG GTG ACC 1682Ser Ser Val Met Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Thr136513701375AAG CAG CTG AAC GAC ACC ACC GGC AAG TTC AAG GAC GTG AGC CAC CTG1730Lys Gln Leu Asn Asp Thr Thr Gly Lys Phe Lys Asp Val Ser His Leu1380138513901395TAC GAC GTG AAG CTG ACC CCC AAG ATG AAC GTG ACC ATC AAG CTG ACC1778Tyr Asp Val Lys Leu Thr Pro Lys Met Asn Val Thr Ile Lys Leu Ser140014051410ATC CTG TAC GAC AAC GCC GAG AGC AAC GAC AAC AGC ATC GGC AAG TGG1826Ile Leu Tyr Asp Asn Ala Glu Ser Asn Asp Asn Ser Ile Gly Lys Trp141514201425ACC AAC ACC AAC ATC GTG AGC GGC GGC AAC AAC GGC AAG AAG CAG TAC1874Thr Asn Thr Asn Ile Val Ser Gly Gly Asn Asn Gly Lys Lys Gln Tyr143014351440AGC AGC AAC AAC CCC GAC GCC AAC CTG ACC CTG AAC ACC GAC GCC CAG1922Ser Ser Asn Asn Pro Asp Ala Asn Leu Thr Leu Asn Thr Asp Ala Gln144514501455GAG AAG CTG AAC AAG AAC CGC GAC TAC TAC ATC AGC CTG TAC ATG AAG1970Glu Lys Leu Asn Lys Asn Arg Asp Tyr Tyr Ile Ser Leu Tyr Met Lys1460146514701475AGC GAG AAG AAC ACC CAG TGC GAG ATC ACC ATC GAC GGC GAG ATA TAC2018Ser Glu Lys Asn Thr Gln Cys Glu Ile Thr Ile Asp Gly Glu Ile Tyr148014851490CCC ATC ACC ACC AAG ACC GTG AAC GTG AAC AAG GAC AAC TAC AAG CGC2066Pro Ile Thr Thr Lys Thr Val Asn Val Asn Lys Asp Asn Tyr Lys Arg149515001505CTG GAC ATC ATC GCC CAC AAC ATC AAG AGC AAC CCC ATC AGC AGC CTG2114Leu Asp Ile Ile Ala His Asn Ile Lys Ser Asn Pro Ile Ser Ser Leu151015151520CAC ATC AAG ACC AAC GAC GAG ATC ACC CTG TTC TGG GAC GAC ATA TCG2162His Ile Lys Thr Asn Asp Glu Ile Thr Leu Phe Trp Asp Asp Ile Ser152515301535ATT ACC GAC GTC GCC AGC ATC AAG CCC GAG AAC CTG ACC GAC AGC GAG2210Ile Thr Asp Val Ala Ser Ile Lys Pro Glu Asn Leu Thr Asp Ser Glu1540154515501555ATC AAG CAG ATA TAC AGT CGC TAC GGC ATC AAG CTG GAG GAC GGC ATC2258Ile Lys Gln Ile Tyr Ser Arg Tyr Gly Ile Lys Leu Glu Asp Gly Ile156015651570CTG ATC GAC AAG AAA GGC GGC ATC CAC TAC GGC GAG TTC ATC AAC GAG2306Leu Ile Asp Lys Lys Gly Gly Ile His Tyr Gly Glu Phe Ile Asn Glu157515801585GCC AGC TTC AAC ATC GAG CCC CTG CAG AAC TAC GTG ACC AAG TAC GAG2354Ala Ser Phe Asn Ile Glu Pro Leu Gln Asn Tyr Val Thr Lys Tyr Glu
159015951600GTG ACC TAC AGC AGC GAG CTG GGC CCC AAC GTG AGC GAC ACC CTG GAG 2402Val Thr Tyr Ser Ser Glu Leu Gly Pro Asn Val Ser Asp Thr Leu Glu160516101615AGC GAC AAG ATT TAC AAG GAC GGC ACC ATC AAG TTC GAC TTC ACC AAG 2450Ser Asp Lys Ile Tyr Lys Asp Gly Thr Ile Lys Phe Asp Phe Thr Lys1620162516301635TAC AGC AAG AAC GAG CAG GGC CTG TTC TAC GAC AGC GGC CTG AAC TGG 2498Tyr Ser Lys Asn Glu Gln Gly Leu Phe Tyr Asp Ser Gly Leu Asn Trp164016451650GAC TTC AAG ATC AAC GCC ATC ACC TAC GAC GGC AAG GAG ATG AAC GTG 2546Asp Phe Lys Ile Asn Ala Ile Thr Tyr Asp Gly Lys Glu Met Asn Val165516601665TTC CAC CGC TAC AAC AAG TAGATCTGAG CT 2576Phe His Arg Tyr Asn Lys1670(2)关于SEQ ID NO:36的信息(ⅰ)序列特征(A)长度852个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:36:Met Lys Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln Lys Glu1 5 10 15Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe20 25 30Ser Asn Leu Thr Met Phe Ala Pro Thr Arg Asp Ser Thr Leu Ile Tyr35 40 45Asp Gln Gln Thr Ala Asn Lys Leu Leu Asp Lys Lys Gln Gln Glu Tyr50 55 60Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln Ser Lys Glu Thr Gly Asp65 70 75 80Phe Thr Phe Asn Leu Ser Glu Asp Glu Gln Ala Ile Ile Glu Ile Asn85 90 95Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu Lys Gln Val Val His Leu100 105 110Glu Lys Gly Lys Leu Val Pro Ile Lys Ile Glu Tyr Gln Ser Asp Thr115 120 125Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys Glu Leu Lys Leu Phe Lys130 135 140Ile Asp Ser Gln Asn Gln Pro Gln Gln Val Gln Gln Asp Glu Leu Arg145 150 155 160Asn Pro Glu Phe Asn Lys Lys Glu Ser Gln Glu Phe Leu Ala Lys Pro165 170 175Ser Lys Ile Asn Leu Phe Thr Gln Gln Met Lys Arg Glu Ile Asp Glu180 185 190Asp Thr Asp Thr Asp Gly Asp Ser Ile Pro Asp Leu Trp Glu Glu Asn195 200 205Gly Tyr Thr Ile Gln Asn Arg Ile Ala Val Lys Trp Asp Asp Ser Leu210 215 220Ala Ser Lys Gly Tyr Thr Lys Phe Val Ser Asn Pro Leu Glu Ser His225 230 235 240Thr Val Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ala Arg Asp Leu245 250 255Asp Leu Ser Asn Ala Lys Glu Thr Phe Asn Pro Leu Val Ala Ala Phe260 265 270Pro Ser Val Asn Val Ser Met Glu Lys Val Ile Leu Ser Pro Asn Glu275 280 285Asn Leu Ser Asn Ser Val Glu Ser His Ser Ser Thr Asn Trp Ser Tyr290 295 300Thr Asn Thr Glu Gly Ala Ser Val Glu Ala Gly Ile Gly Pro Lys Gly305 310 315 320Ile Ser Phe Gly Val Ser Val Asn Tyr Gln His Ser Glu Thr Val Ala325 330 335Gln Glu Trp Gly Thr Ser Thr Gly Asn Thr Ser Gln Phe Asn Thr Ala340 345 350Ser Ala Gly Tyr Leu Asn Ala Asn Val Arg Tyr Asn Asn Val Gly Thr355 360 365Gly Ala Ile Tyr Asp Val Lys Pro Thr Thr Ser Phe Val Leu Asn Asn370 375 380Asp Thr Ile Ala Thr Ile Thr Ala Lys Ser Asn Ser Thr Ala Leu Asn385 390 395 400Ile Ser Pro Gly Glu Ser Tyr Pro Lys Lys Gly Gln Asn Gly Ile Ala
405 410 415Ile Thr Ser Met Asp Asp Phe Asn Ser His Pro Ile Thr Leu Asn Lys420 425 430Lys Gln Val Asp Asn Leu Leu Asn Asn Lys Pro Met Met Leu Glu Thr435 440 445Asn Gln Thr Asp Gly Val Tyr Lys Ile Lys Asp Thr His Gly Asn Ile450 455 460Val Thr Gly Gly Glu Trp Asn Gly Val Ile Gln Gln Ile Lys Ala Lys465 470 475 480Thr Ala Ser Ile Ile Val Asp Asp Gly Glu Arg Val Ala Glu Lys Arg485 490 495Val Ala Ala Lys Asp Tyr Glu Asn Pro Glu Asp Lys Thr Pro Ser Leu500 505 510Thr Leu Lys Asp Ala Leu Lys Leu Ser Tyr Pro Asp Glu Ile Lys Glu515 520 525Ile Glu Gly Leu Leu Tyr Tyr Lys Asn Lys Pro Ile Tyr Glu Ser Ser530 535 540Val Met Thr Tyr Leu Asp Glu Asn Thr Ala Lys Glu Val Thr Lys Gln545 550 555 560Leu Asn Asp Thr Thr Gly Lys Phe Lys Asp Val Ser His Leu Tyr Asp565 570 575Val Lys Leu Thr Pro Lys Met Asn Val Thr Ile Lys Leu Ser Ile Leu580 585 590Tyr Asp Asn Ala Glu Ser Asn Asp Asn Ser Ile Gly Lys Trp Thr Asn595 600 605Thr Asn Ile Val Ser Gly Gly Asn Asn Gly Lys Lys Gln Tyr Ser Ser610 615 620Asn Asn Pro Asp Ala Asn Leu Thr Leu Asn Thr Asp Ala Gln Glu Lys625 630 635 640Leu Asn Lys Asn Arg Asp Tyr Tyr Ile Ser Leu Tyr Met Lys Ser Glu645 650 655Lys Asn Thr Gln Cys Glu Ile Thr Ile Asp Gly Glu Ile Tyr Pro Ile660 665 670Thr Thr Lys Thr Val Asn Val Asn Lys Asp Asn Tyr Lys Arg Leu Asp675 680 685Ile Ile Ala His Asn Ile Lys Ser Asn Pro Ile Ser Ser Leu His Ile690 695 700Lys Thr Asn Asp Glu Ile Thr Leu Phe Trp Asp Asp Ile Ser Ile Thr705710 715 720Asp Val Ala Ser Ile Lys Pro Glu Asn Leu Thr Asp Ser Glu Ile Lys725 730 735Gln Ile Tyr Ser Arg Tyr Gly Ile Lys Leu Glu Asp Gly Ile Leu Ile740 745 750Asp Lys Lys Gly Gly Ile His Tyr Gly Glu Phe Ile Asn Glu Ala Ser755 760 765Phe Asn Ile Glu Pro Leu Gln Asn Tyr Val Thr Lys Tyr Glu Val Thr770 775 780Tyr Ser Ser Glu Leu Gly Pro Asn Val Ser Asp Thr Leu Glu Ser Asp785 790 795 800Lys Ile Tyr Lys Asp Gly Thr Ile Lys Phe Asp Phe Thr Lys Tyr Ser805 810 815Lys Asn Glu Gln Gly Leu Phe Tyr Asp Ser Gly Leu Asn Trp Asp Phe820 825 830Lys Ile Asn Ala Ile Thr Tyr Asp Gly Lys Glu Met Asn Val Phe His835 840 845Arg Tyr Asn Lys850(2)关于SEQ ID NO:37的信息(ⅰ)序列特征(A)长度32个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于制备pCIB5527的正向引物”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:37:GGATCCACCA TGCTGCAGAA CCTGAAGATC AC 32(2)关于SEQ ID NO:38的信息(ⅰ)序列特征
(A)长度18个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于制备pCIB5527的反向引物”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:38:AAGCTTCCAC TCCTTCTC 18(2)关于SEQ ID NO:39的信息(ⅰ)序列特征(A)长度1241个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置9..1238(D)其它信息/备注=“编码VIP2A(a)和切出的芽孢杆菌属分泌信号的玉米最佳化DNA序列，包含在pCIB5527中。”(ⅹⅰ)序列描述SEQ ID NO:39:GATCCACC ATG CTG CAG AAC CTG AAG ATC ACC GAC AAG GTG GAG GAC TTC50Met Leu GLn Asn Leu Lys Ile Thr Asp Lys Val Glu Asp Phe855 860 865AAG GAG GAC AAG GAG AAG GCC AAG GAG TGG GGC AAG GAG AAG GAG AAG 98Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys Glu Lys870 875 880GAG TGG AAG CTT ACC GCC ACC GAG AAG GGC AAG ATG AAC AAC TTC CTG146Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn Phe Leu
885 890 895GAC AAC AAG AAC GAC ATC AAG ACC AAC TAC AAG GAG ATC ACC TTC AGC194Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr Phe Ser900 905 910ATA GCC GGC AGC TTC GAG GAC GAG ATC AAG GAC CTG AAG GAG ATC GAC242Ile Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu Ile Asp915 920 925 930AAG ATG TTC GAC AAG ACC AAC CTG AGC AAC AGC ATC ATC ACC TAC AAG290Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr Tyr Lys935 940 945AAC GTG GAG CCC ACC ACC ATC GGC TTC AAC AAG AGC CTG ACC GAG GGC338Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr Glu Gly950 955 960AAC ACC ATC AAC AGC GAC GCC ATG GCC CAG TTC AAG GAG CAG TTC CTG386Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln Phe Leu965 970 975GAC CGC GAC ATC AAG TTC GAC AGC TAC CTG GAC ACC CAC CTG ACC GCC434Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu Thr Ala980 985 990CAG CAG GTG AGC AGC AAG GAG CGC GTG ATC CTG AAG GTG ACC GTC CCC482Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr Val Pro995 100010051010AGC GGC AAG GGC AGC ACC ACC CCC ACC AAG GCC GGC GTG ATC CTG AAC530Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile Leu Asn101510201025AAC AGC GAG TAC AAG ATG CTG ATC GAC AAC GGC TAC ATG GTG CAC GTG578Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val His Val103010351040GAC AAG GTG AGC AAG GTG GTG AAG AAG GGC GTG GAG TGC CTC CAG ATC626Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu Gln Ile104510501055GAG GGC ACC CTG AAG AAG AGT CTA GAC TYC AAG AAC GAC ATC AAC GCC674Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile Asn Ala106010651070GAG GCC CAC AGC TGG GGC ATG AAG AAC TAC GAG GAG TGG GCC AAG GAC722Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala Lys Asp1075108010851090CTG ACC GAC AGC CAG CGC GAG GCC CTG GAC GGC TAC GCC CGC CAG GAC770Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg Gln Asp109511001105TAC AAG GAG ATC AAC AAC TAC CTG CGC AAC CAG GGC GGC AGC GGC AAC818Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser Gly Asn111011151120GAG AAG CTG GAC GCC CAG ATC AAG AAC ATC AGC GAC GCC CTG GGC AAG 866Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu Gly Lys112511301135AAG CCC ATC CCC GAG AAC ATC ACC GTG TAC CGC TGG TGC GGC ATG CCC 914Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly Met Pro114011451150GAG TTC GGC TAC CAG ATC AGC GAC CCC CTG CCC AGC CTG AAG GAC TTC 962Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys Asp Phe1155116011651170GAG GAG CAG TTC CTG AAC ACC ATC AAG GAG GAC AAG GGC TAC ATG AGC1010Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr Met Ser117511801185ACC AGC CTG AGC AGC GAG CGC CTG GCC GCC TTC GGC AGC CGC AAG ATC1058Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg Lys Ile119011951200ATC CTG CGC CTG CAG GTG CCC AAG GGC AGC ACT GGT GCC TAC CTG AGC1106Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr Leu Ser120512101215GCC ATC GGC GGC TTC GCC AGC GAG AAG GAG ATC CTG CTG GAT AAG GAC1154Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp Lys Asp122012251230AGC AAG TAC CAC ATC GAC AAG GTG ACC GAG GTG ATC ATC AAG GGC GTG1202Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys Gly Val1235124012451250AAG CGC TAC GTG GTG GAC GCC ACC CTG CTG ACC AAC TAG1241Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn12551260(2)关于SEQ ID NO:40的信息(ⅰ)序列特征(A)长度410个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:40:Met Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu Asp Phe Lys Glu1 5 10 15Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys Glu Lys Glu Trp20 25 30Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn Phe Leu Asp Asn
35 40 45Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr Phe Ser Ile Ala50 55 60Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu Ile Asp Lys Met65 70 75 80Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr Tyr Lys Asn Val85 90 95Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr Glu Gly Asn Thr100 105 110Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln Phe Leu Asp Arg115 120 125Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu Thr Ala Gln Gln130 135 140Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr Val Pro Ser Gly145 150 155 160Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile Leu Asn Asn Ser165 170 175Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val His Val Asp Lys180 185 190Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu Gln Ile Glu Gly195 200 205Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile Asn Ala Glu Ala210 215 220His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala Lys Asp Leu Thr225 230 235 240Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg Gln Asp Tyr Lys245 250 255Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser Gly Asn Glu Lys260 265 270Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu Gly Lys Lys Pro275 280 285Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly Met Pro Glu Phe290 295 300Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys Asp Phe Glu Glu305 310 315 320Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr Met Ser Thr Ser325 330 335Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg Lys Ile Ile Leu340 345 350Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr Leu Ser Ala Ile355 360 365Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp Lys Asp Ser Lys370 375 380Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys Gly Val Lys Arg385 390 395 400Tyr Val Val Asp Ala Thr Leu Leu Thr Asn405 410(2)关于SEQ ID NO:41的信息(ⅰ)序列特征(A)长度72个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于构建pCIB5527的编码真核生物分泌信号的寡核苷酸”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:41:GGATCCACCA TGGGCTGGAG CTGGATCTTC CTGTTCCTGC TGAGCGGCGC CGCGGGCGTG 60CACTGCCTGC AG 72(2)关于SEQ ID NO:42的信息(ⅰ)序列特征(A)长度1241个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸
(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置9..1238(D)其它信息/备注=“编码VIP2A(a)和切出的芽孢杆菌属分泌信号及插入的真核生物分泌信号的玉米最佳化DNA序列，包含于pCIB5528中。”(ⅹⅰ)序列描述SEQ ID NO:42:GATCCACC ATG CTG CAG AAC CTG AAG ATC ACC GAC AAG GTG GAG GAC TTC 50Met Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu Asp Phe415 420AAG GAG GAC AAG GAG AAG GCC AAG GAG TGG GGC AAG GAG AAG GAG AAG98Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys Glu Lys425 430 435 440GAG TGG AAG CTT ACC GCC ACC GAG AAG GGC AAG ATG AAC AAC TTC CTG 146Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn Phe Leu445 450 455GAC AAC AAG AAC GAC ATC AAG ACC AAC TAC AAG GAG ATC ACC TTC AGC 194Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr Phe Ser460 465 470ATA GCC GGC AGC TTC GAG GAC GAG ATC AAG GAC CTG AAG GAG ATC GAC 242Ile Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu Tle Asp475 480 485AAG ATG TTC GAC AAG ACC AAC CTG AGC AAC AGC ATC ATC ACC TAC AAG 290Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr Tyr Lys490 495 500AAC GTG GAG CCC ACC ACC ATC GGC TTC AAC AAG AGC CTG ACC GAG GGC 338Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr Glu Gly505 510 515 520AAC ACC ATC AAC AGC GAC GCC ATG GCC CAG TTC AAG GAG CAG TTC CTG 386Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln Phe Leu525 530 535GAC CGC GAC ATC AAG TTC GAC AGC TAC CTG GAC ACC CAC CTG ACC GCC 434Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu Thr Ala540 545 550CAG CAG GTG AGC AGC AAG GAG CGC GTG ATC CTG AAG GTG ACC GTC CCC 482Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr Val Pro555 560 565AGC GGC AAG GGC AGC ACC ACC CCC ACC AAG GCC GGC GTG ATC CTG AAC 530Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile Leu Asn570 575 580AAC AGC GAG TAC AAG ATG CTG ATC GAC AAC GGC TAC ATG GTG CAC GTG578Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val His Val585 590 595 600GAC AAG GTG AGC AAG GTG GTG AAG AAG GGC GTG GAG TGC CTC CAG ATC626Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu Gln Ile605 610 615GAG GGC ACC CTG AAG AAG AGT CTA GAC TTC AAG AAC GAC ATC AAC GCC674Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile Asn Ala620 625 630GAG GCC CAC AGC TCG GGC ATG AAG AAC TAC GAG GAG TGG GCC AAG GAC722Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala Lys Asp635 640 645CTG ACC GAC AGC CAG CGC GAG GCC CTG GAC GGC TAC GCC CGC CAG GAC770Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg Gln Asp650 655 660TAC AAG GAG ATC AAC AAC TAC CTG CGC AAC CAG GGC GGC AGC GGC AAC818Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser Gly Asn665 670 675 680GAG AAG CTG GAC GCC CAG ATC AAG AAC ATC AGC GAC GCC CTG GGC AAG866Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu Gly Lys685 690 695AAG CCC ATC CCC GAG AAC ATC ACC GTG TAC CGC TGG TGC GGC ATG CCC914Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly Met Pro700 705 710GAG TTC GGC TAC CAG ATC AGC GAC CCC CTG CCC AGC CTG AAG GAC TTC962Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys Asp Phe715 720 725GAG GAG CAG TTC CTG AAC ACC ATC AAG GAG GAC AAG GGC TAC ATG AGC 1010Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr Met Ser730 735 740ACC AGC CTG AGC AGC GAG CGC CTG GCC GCC TTC GGC AGC CGC AAG ATC 1058Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg Lys Ile745 750 755 760ATC CTG CGC CTG CAG GTG CCC AAG GGC AGC ACT GGT GCC TAC CTG AGC 1106Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr Leu Ser765 770 775GCC ATC GGC GGC TTC GCC AGC GAG AAG GAG ATC CTG CTG GAT AAG GAC 1154Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp Lys Asp780 785 790AGC AAG TAC CAC ATC GAC AAG GTG ACC GAG GTG ATC ATC AAG GGC GTG 1202Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys Gly Val795 800 805AAG CGC TAC GTG GTG GAC GCC ACC CTG CTG ACC AAC TAG 1241Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn810 815 820(2)关于SEQ ID NO:43的信息(ⅰ)序列特征(A)长度410个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:43:Met Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu Asp Phe Lys Glu1 5 10 15Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys Glu Lys Glu Trp20 25 30Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn Phe Leu Asp Asn35 40 45Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr Phe Ser Ile Ala50 55 60Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu Ile Asp Lys Met65 70 75 80Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr Tyr Lys Asn Val85 90 95Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr Glu Gly Asn Thr100 105 110Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln Phe Leu Asp Arg115 120 125Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu Thr Ala Gln Gln130 135 140Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr Val Pro Ser Gly145 150 155 160Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile Leu Asn Asn Ser165 170 175Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val His Val Asp Lys180 185 190Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu Gln Ile Glu Gly195 200 205Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile Asn Ala Glu Ala
210 215 220His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala Lys Asp Leu Thr225 230 235 240Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg Gln Asp Tyr Lys245 250 255Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser Gly Asn Glu Lys260 265 270Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu Gly Lys Lys Pro275 280 285Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly Met Pro Glu Phe290 295 300Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys Asp Phe Glu Glu305 310 315 320Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr Met Ser Thr Ser325 330 335Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg Lys Ile Ile Leu340 345 350Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr Leu Ser Ala Ile355 360 365Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp Lys Asp Ser Lys370 375 380Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys Gly Val Lys Arg385 390 395 400Tyr Val Val Asp Ala Thr Leu Leu Thr Asn405 410(2)关于SEQ ID NO:44的信息(ⅰ)序列特征(A)长度86个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于构建pCIB5533的编码液泡导向肽的寡核苷酸”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:44:CCGCGGGCGT GCACTGCCTC AGCAGCAGCA GCTTCGCCGA CAGCAACCCC ATCCGCGTGA 60CCGACCGCGC CGCCAGCACC CTGCAG86(2)关于SEQ ID NO:45的信息(ⅰ)序列特征(A)长度1358个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置9..1335(D)其它信息/备注=“编码VIP2A(a)和切出的芽孢杆菌分泌信号及插入的液泡导向肽的玉米最佳化DNA序列，包含于pCIB5533中。”(ⅹⅰ)序列描述SEQ ID NO:45:GATCCACC ATG GGC TGG AGC TGG ATC TTC CTG TTC CTG CTG AGC GGC GCC50Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala415 420GCG GGC GTG CAC TGC CTC AGC AGC AGC AGC TTC GCC GAC AGC AAC CCC 98Ala Gly Val His Cys Leu Ser Ser Ser Ser Phe Ala Asp Ser Asn Pro425 430 435 440ATC CGC GTG ACC GAC CGC GCC GCC AGC ACC CTG CAG AAC CTG AAG ATC146Ile Arg Val Thr Asp Arg Ala Ala Ser Thr Leu Gln Asn Leu Lys Ile445 450 455ACC GAC AAG GTG GAG GAC TTC AAG GAG GAC AAG GAG AAG GCC AAG GAG194Thr Asp Lys Val Glu Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu460 465 470TGG GGC AAG GAG AAG GAG AAG GAG TGG AAG CTT ACC GCC ACC GAG AAG242Trp Gly Lys Glu Lys Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys475 480 485GGC AAG ATG AAC AAC TTC CTG GAC AAC AAG AAC GAC ATC AAG ACC AAC290Gly Lys Met Asn Asn Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn490 495 500TAC AAG GAG ATC ACC TTC AGC ATA GCC GGC AGC TTC GAG GAC GAG ATC338Tyr Lys Glu Ile Thr Phe Ser Ile Ala Gly Ser Phe Glu Asp Glu Ile505 510 515 520AAG GAC CTG AAG GAG ATC GAC AAG ATG TTC GAC AAG ACC AAC CTG AGC386Lys Asp Leu Lys Glu Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser525 530 535AAC AGC ATC ATC ACC TAC AAG AAC GTG GAG CCC ACC ACC ATC GGC TTC434Asn Ser Ile Ile Thr Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe540 545 550AAC AAG AGC CTG ACC GAG GGC AAC ACC ATC AAC AGC GAC GCC ATG GCC482Asn Lys Ser Leu Thr Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala555 560 565CAG TTC AAG GAG CAG TTC CTG GAC CGC GAC ATC AAG TTC GAC AGC TAC530Gln Phe Lys Glu Gln Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr570 575 580CTG GAC ACC CAC CTG ACC GCC CAG CAG GTG AGC AGC AAG GAG CGC GTG578Leu Asp Thr His Leu Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val585 590 595 600ATC CTG AAG GTG ACC GTC CCC AGC GGC AAG GGC AGC ACC ACC CCC ACC626Ile Leu Lys Val Thr Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr605 610 615AAG GCC GGC GTG ATC CTG AAC AAC AGC GAG TAC AAG ATG CTG ATC GAC674Lys Ala Gly Val Ile Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp620 625 630AAC GGC TAC ATG GTG CAC GTG GAC AAG GTG AGC AAG GTG GTG AAG AAG722Asn Gly Tyr Met Val His Val Asp Lys Val Ser Lys Val Val Lys Lys635 640 645GGC GTG GAG TGC CTC CAG ATC GAG GGC ACC CTG AAG AAG AGT CTA GAC770Gly Val Glu Cys Leu Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp650 655 660TTC AAG AAC GAC ATC AAC GCC GAG GCC CAC AGC TGG GGC ATG AAG AAC818Phe Lys Asn Asp Ile Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn665 670 675 680TAC GAG GAG TGG GCC AAG GAC CTG ACC GAC AGC CAG CGC GAG GCC CTG866Tyr Glu Glu Trp Ala Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu685 690 695GAC GGC TAC GCC CGC CAG GAC TAC AAG GAG ATC AAC AAC TAC CTG CGC914Asp Gly Tyr Ala Arg Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg700 705 710AAC CAG GGC GGC AGC GGC AAC GAG AAG CTG GAC GCC CAG ATC AAG AAC962Asn Gln Gly Gly Ser Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn715 720 725ATC AGC GAC GCC CTG GGC AAG AAG CCC ATC CCC GAG AAC ATC ACC GTG 1010Ile Ser Asp Ala Leu Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val730 735 740TAC CGC TGG TGC GGC ATG CCC GAG TTC GGC TAC CAG ATC AGC GAC CCC 1058Tyr Arg Trp Cys Gly Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro745 750 755 760CTG CCC AGC CTG AAG GAC TTC GAG GAG CAG TTC CTG AAC ACC ATC AAG 1106Leu Pro Ser Leu Lys Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys765 770 775GAG GAC AAG GGC TAC ATG AGC ACC AGC CTG AGC AGC GAG CGC CTG GCC 1154Glu Asp Lys Gly Tyr Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala780 785 790GCC TTC GGC AGC CGC AAG ATC ATC CTG CGC CTG CAG GTG CCC AAG GGC 1202Ala Phe Gly Ser Arg Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly795 800 805AGC ACT GGT GCC TAC CTG AGC GCC ATC GGC GGC TTC CCC AGC GAG AAG 1250Ser Thr Gly Ala Tyr Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys810 815 820GAG ATC CTG CTG GAT AAG GAC AGC AAG TAC CAC ATC GAC AAG GTG ACC 1298Glu Ile Leu Leu Asp Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr825 830 835 840GAG GTG ATC ATC AAG GGC GTG AAG CGC TAC GTG GTG GAC GCC ACC CTG 1346Glu Val Ile Ile Lys Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu845 850 855CTG ACC AAC TAG 1358Leu Thr Asn(2)关于SEQ ID NO:46的信息(ⅰ)序列特征(A)长度449个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:46:Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly1 5 10 15Val His Cys Leu Ser Ser Ser Ser Phe Ala Asp Ser Asn Pro Ile Arg20 25 30Val Thr Asp Arg Ala Ala Ser Thr Leu Gln Asn Leu Lys Ile Thr Asp
35 40 45Lys Val Glu Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly50 55 60Lys Glu Lys Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys65 70 75 80Met Asn Asn Phe Leu Asp Ash Lys Asn Asp Ile Lys Thr Asn Tyr Lys85 90 95Glu Ile Thr Phe Ser Ile Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp100 105 110Leu Lys Glu Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser115 120 125Ile Ile Thr Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys130 135 140Ser Leu Thr Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe145 150 155 160Lys Glu Gln Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp165 170 175Thr His Leu Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu180 185 190Lys Val Thr Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala195 200 205Gly Val Ile Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly210 215 220Tyr Met Val His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val225 230 235 240Glu Cys Leu Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys245 250 255Asn Asp Ile Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu260 265 270Glu Trp Ala Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly275 280 285Tyr Ala Arg Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln290 295 300Gly Gly Ser Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser305 310 315 320Asp Ala Leu Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg325 330 335Trp Cys Gly Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro340 345 350Ser Leu Lys Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp355 360 365Lys Gly Tyr Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe370 375 380Gly Ser Arg Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr385 390 395 400Gly Ala Tyr Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile405 410 415Leu Leu Asp Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val420 425 430Ile Ile Lys Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr435 440 445Asn(2)关于SEQ ID NO:47的信息(ⅰ)序列特征(A)长度16个氨基酸(B)类型氨基酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型肽(ⅲ)假拟否(ⅸ)特征(A)名称/键肽(B)位置1..16(D)其它信息/备注=“用于构建pCIB5533，融合VIP1A(a)和VIP2A(a)”的连接肽(ⅹⅰ)序列描述SEQ ID NO:47:Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro Thr Pro Ser1 5 10 15(2)关于SEQ ID NO:48的信息(ⅰ)序列特征(A)长度66个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“用于构建pCIB553的连接肽DNA”(ⅲ)假拟否(ⅹⅰ)序列描述SEQ ID NO:48:CCCGGGCCTT CTACTCCCCC AACTCCCTCT CCTAGCACGC CTCCGACACC TAGCGATATC 60GGATCC 66(2)关于SEQ ID NO:49的信息(ⅰ)序列特征(A)长度4031个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型其它核酸(A)描述/desc=“合成的DNA”(ⅲ)假拟否(ⅸ)特征(A)名称/键CDS(B)位置6..4019(D)其它信息/备注=“编码VIP2A(a)-VIP1A(a)融合蛋白的玉米最佳化DNA序列，包含于pCIB5531中。”(ⅹⅰ)序列描述SEQ ID NO:49:GATCC ATG AAG CGC ATG GAG GGC AAG CTG TTC ATG GTG AGC AAG AAG 47Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys450 455 460CTC CAG GTG GTG ACC AAG ACC GTG CTG CTG AGC ACC GTG TTC AGC ATC95Leu Gln Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile
465 470 475AGC CTG CTG AAC AAC GAG GTG ATC AAG GCC GAG CAG CTG AAC ATC AAC143Ser Leu Leu Asn Asn Glu Val Ile Lys Ala Glu Gln Leu Asn Ile Asn480 485 490 495AGC CAG AGC AAG TAC ACC AAC CTC CAG AAC CTG AAG ATC ACC GAC AAG191Ser Gln Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Thr Asp Lys500 505 510GTG GAG GAC TTC AAG GAG GAC AAG GAG AAG GCC AAG GAG TGG GGC AAG239Val Glu Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys515 520 525GAG AAG GAG AAG GAG TGG AAG CTT ACC GCC ACC GAG AAG GGC AAG ATG287Glu Lys Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met530 535 540AAC AAC TTC CTG GAC AAC AAG AAC GAC ATC AAG ACC AAC TAC AAG GAG335Asn Asn Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu545 550 555ATC ACC TTC AGC ATA GCC GGC AGC TTC GAG GAC GAG ATC AAG GAC CTG383Ile Thr Phe Ser Ile Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu560 565 570 575AAG GAG ATC GAC AAG ATG TTC GAC AAG ACC AAC CTG AGC AAC AGC ATC431Lys Glu Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile580 585 590ATC ACC TAC AAG AAC GTG GAG CCC ACC ACC ATC GGC TTC AAC AAG AGC479Ile Thr Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser595 600 605CTG ACC GAG GGC AAC ACC ATC AAC AGC GAC GCC ATG GCC CAG TTC AAG527Leu Thr Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys610 615 620GAG CAG TTC CTG GAC CGC GAC ATC AAG TTC GAC AGC TAC CTG GAC ACC575Glu Gln Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr625 630 635CAC CTG ACC GCC CAG CAG GTG AGC AGC AAG GAG CGC GTG ATC CTG AAG623His Leu Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys640 645 650 655GTG ACC GTC CCC AGC GGC AAG GGC AGC ACC ACC CCC ACC AAG GCC GGC671Val Thr Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly660 665 670GTG ATC CTG AAC AAC AGC GAG TAC AAG ATG CTG ATC GAC AAC GGC TAC719Val Ile Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr675 680 685ATG GTG CAC GTG GAC AAG GTG AGC AAG GTG GTG AAG AAG GGC GTG GAG767Met Val His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu690 695 700TGC CTC CAG ATC GAG GGC ACC CTG AAG AAG AGT CTA GAC TTC AAG AAC815Cys Leu Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn705 710 715GAC ATC AAC GCC GAG GCC CAC AGC TGG GGC ATG AAG AAC TAC GAG GAG863Asp Ile Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu720 725 730 735TGG GCC AAG GAC CTG ACC GAC AGC CAG CGC GAG GCC CTG GAC GGC TAC911Trp Ala Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr740 745 750GCC CGC CAG GAC TAC AAG GAG ATC AAC AAC TAC CTG CGC AAC CAG GGC959Ala Arg Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly755 760 765GGC AGC GGC AAC GAG AAG CTG GAC GCC CAG ATC AAG AAC ATC AGC GAC 1007Gly Ser Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp770 775 780GCC CTG GGC AAG AAG CCC ATC CCC GAG AAC ATC ACC GTG TAC CGC TGG 1055Ala Leu Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp785 790 795TGC GGC ATG CCC GAG TTC GGC TAC CAG ATC AGC GAC CCC CTG CCC AGC 1103Cys Gly Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser800 805 810 815CTG AAG GAC TTC GAG GAG CAG TTC CTG AAC ACC ATC AAG GAG GAC AAG 1151Leu Lys Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys820 825 830GGC TAC ATG AGC ACC AGC CTG AGC AGC GAG CGC CTG GCC GCC TTC GGC 1199Gly Tyr Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly835 840 845AGC CGC AAG ATC ATC CTG CGC CTG CAG GTG CCC AAG GGC AGC ACT GGT 1247Ser Arg Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly850 855 860GCC TAC CTG AGC GCC ATC GGC GGC TTC GCC AGC GAG AAG GAG ATC CTG 1295Ala Tyr Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu865 870 875CTG GAT AAG GAC AGC AAG TAC CAC ATC GAC AAG GTG ACC GAG GTG ATC 1343Leu Asp Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile880 885 890 895ATC AAG GGC GTG AAG CGC TAC GTG GTG GAC GCC ACC CTG CTG ACC AAC 1391Ile Lys Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn900 905 910TCC CGG GGG CCT TCT ACT CCC CCA ACT CCC TCT CCT AGC ACG CCr CCG 1439Ser Arg Gly Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro915 920 925ACA CCT AGC GAT ATC GGA TCC ACC ATG AAG ACC AAC CAG ATC AGC ACC 1487Thr Pro Ser Asp Ile Gly Ser Thr Met Lys Thr Asn Gln Ile Ser Thr930 935 940ACC CAG AAG AAC CAG CAG AAG GAG ATG GAC CGC AAG GGC CTG CTG GGC1535Thr Gln Lys Asn Gln Gln Lys Glu Met Asp Arg Lys Gly Leu Leu Gly945 950 955TAC TAC TTC AAG GGC AAG GAC TTC AGC AAC CTG ACC ATG TTC GCC CCC1583Tyr Tyr Phe Lys Gly Lys Asp Phe Ser Asn Leu Thr Met Phe Ala Pro960 965 970 975ACG CGT GAC AGC ACC CTG ATC TAC GAC CAG CAG ACC GCC AAC AAG CTG1631Thr Arg Asp Ser Thr Leu Ile Tyr Asp Gln Gln Thr Ala Asn Lys Leu980 985 990CTG GAC AAG AAG CAG CAG GAG TAC CAG AGC ATC CGC TGG ATC GGC CTG1679Leu Asp Lys Lys Gln Gln Glu Tyr Gln Ser Ile Arg Trp Ile Gly Leu995 10001005ATC CAG AGC AAG GAG ACC GGC GAC TTC ACC TTC AAC CTG AGC GAG GAC1727Ile Gln Ser Lys Glu Thr Gly Asp Phe Thr Phe Asn Leu Ser Glu Asp101010151020GAG CAG GCC ATC ATC GAG ATC AAC GGC AAG ATC ATC AGC AAC AAG GGC1775Glu Gln Ala Ile Ile Glu Ile Asn Gly Lys Ile Ile Ser Asn Lys Gly102510301035AAG GAG AAG CAG GTG GTG CAC CTG GAG AAG GGC AAG CTG GTG CCC ATC1823Lys Glu Lys Gln Val Val His Leu Glu Lys Gly Lys Leu Val Pro Ile1040104510501055AAG ATC GAG TAC CAG AGC GAC ACC AAG TTC AAC ATC GAC AGC AAG ACC1871Lys Ile Glu Tyr Gln Ser Asp Thr Lys Phe Asn Ile Asp Ser Lys Thr106010651070TTC AAG GAG CTG AAG CTT TTC AAG ATC GAC AGC CAG AAC CAG CCC CAG1919Phe Lys Glu Leu Lys Leu Phe Lys Ile Asp Ser Gln Asn Gln Pro Gln107510801085CAG GTG CAG CAG GAC GAG CTG CGC AAC CCC GAG TTC AAC AAG AAG GAG1967Gln Val Gln Gln Asp Glu Leu Arg Asn Pro Glu Phe Asn Lys Lys Glu109010951100AGC CAG GAG TTC CTG GCC AAG CCC AGC AAG ATC AAC CTG TTC ACC CAG2015Ser Gln Glu Phe Leu Ala Lys Pro Ser Lys Ile Asn Leu Phe Thr Gln110511101115CAG ATG AAG CGC GAG ATC GAC GAG GAC ACC GAC ACC GAC GGC GAC AGC2063Gln Met Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser1120112511301135ATC CCC GAC CTG TGG GAG GAG AAC GGC TAC ACC ATC CAG AAC CGC ATC2111Ile Pro Asp Leu Trp Glu Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile114011451150GCC GTG AAG TGG GAC GAC AGC CTG GCT AGC AAG GGC TAC ACC AAG TTC2159Ala Val Lys Trp Asp Asp Ser Leu Ala Ser Lys Gly Tyr Thr Lys Phe
115511601165GTG AGC AAC CCC CTG GAG AGC CAC ACC GTG GGC GAC CCC TAC ACC GAC 2207Val Ser Asn Pro Leu Glu Ser His Thr Val Gly Asp Pro Tyr Thr Asp117011751180TAC GAG AAG GCC GCC CGC GAC CTG GAC CTG AGC AAC GCC AAG GAG ACC 2255Tyr Glu Lys Ala Ala Arg Asp Leu Asp Leu Ser Asn Ala Lys Glu Thr118511901195TTC AAC CCC CTG GTG GCC GCC TTC CCC AGC GTG AAC GTG AGC ATG GAG 2303Phe Asn Pro Leu Val Ala Ala Phe Pro Ser Val Asn Val Ser Met Glu1200120512101215AAG GTG ATC CTG AGC CCC AAC GAG AAC CTG AGC AAC AGC GTG GAG AGC 2351Lys Val Ile Leu Ser Pro Asn Glu Asn Leu Ser Asn Ser Val Glu Ser122012251230CAC TCG AGC ACC AAC TGG AGC TAC ACC AAC ACC GAG GGC GCC AGC GTG 2399His Ser Ser Thr Asn Trp Ser Tyr Thr Asn Thr Glu Gly Ala Ser Val123512401245GAG GCC GGC ATC GGT CCC AAG GGC ATC AGC TTC GGC GTG AGC GTG AAC 2447Glu Ala Gly Ile Gly Pro Lys Gly Ile Ser Phe Gly Val Ser Val Asn125012551260TAC CAG CAC AGC GAG ACC GTG GCC CAG GAG TGG GGC ACC AGC ACC GGC 2495Tyr Gln His Ser Glu Thr Val Ala Gln Glu Trp Gly Thr Ser Thr Gly126512701275AAC ACC AGC CAG TTC AAC ACC GCC AGC GCC GGC TAC CTG AAC GCC AAC 2543Asn Thr Ser Gln Phe Asn Thr Ala Ser Ala Gly Tyr Leu Asn Ala Asn1280128512901295GTG CGC TAC AAC AAC GTG GGC ACC GGC GCC ATC TAC GAC GTG AAG CCC 2591Val Arg Tyr Asn Asn Val Gly Thr Gly Ala Ile Tyr Asp Val Lys Pro130013051310ACC ACC AGC TTC GTG CTG AAC AAC GAC ACC ATC GCC ACC ATC ACC GCC 2639Thr Thr Ser Phe Val Leu Asn Asn Asp Thr Ile Ala Thr Ile Thr Ala131513201325AAG TCG AAT TCC ACC GCC CTG AAC ATC AGC CCC GGC GAG AGC TAC CCC 2687Lys Ser Asn Ser Thr Ala Leu Asn Ile Ser Pro Gly Glu Ser Tyr Pro133013351340AAG AAG GGC CAG AAC GGC ATC GCC ATC ACC AGC ATG GAC GAC TTC AAC 2735Lys Lys Gly Gln Asn Gly Ile Ala Ile Thr Ser Met Asp Asp Phe Asn134513501355AGC CAC CCC ATC ACC CTG AAC AAG AAG CAG GTG GAC AAC CTG CTG AAC 2783Ser His Pro Ile Thr Leu Asn Lys Lys Gln Val Asp Asn Leu Leu Asn1360136513701375AAC AAG CCC ATG ATG CTG GAG ACC AAC CAG ACC GAC GGC GTC TAC AAG 2831Asn Lys Pro Met Met Leu Glu Thr Asn Gln Thr Asp Gly Val Tyr Lys138013851390ATC AAG GAC ACC CAC GGC AAC ATC GTG ACG GGC GGC GAG TGG AAC GGC 2879Ile Lys Asp Thr His Gly Asn Ile Val Thr Gly Gly Glu Trp Asn Gly139514001405GTG ATC CAG CAG ATC AAG GCC AAG ACC GCC AGC ATC ATC GTC GAC GAC 2927Val Ile Gln Gln Ile Lys Ala Lys Thr Ala Ser Ile Ile Val Asp Asp141014151420GGC GAG CGC GTG GCC GAG AAG CGC GTG GCC GCC AAG GAC TAC GAG AAC 2975Gly Glu Arg Val Ala Glu Lys Arg Val Ala Ala Lys Asp Tyr Glu Asn142514301435CCC GAG GAC AAG ACC CCC AGC CTG ACC CTG AAG GAC GCC CTG AAG CTG 3023Pro Glu Asp Lys Thr Pro Ser Leu Thr Leu Lys Asp Ala Leu Lys Leu1440144514501455AGC TAC CCC GAC GAG ATC AAG GAG ATC GAG GGC TTG CTG TAC TAC AAG 3071Ser Tyr Pro Asp Glu Ile Lys Glu Ile Glu Gly Leu Leu Tyr Tyr Lys146014651470AAC AAG CCC ATC TAC GAG AGC AGC GTG ATG ACC TAT CTA GAC GAG AAC 3119Asn Lys Pro Ile Tyr Glu Ser Ser Val Met Thr Tyr Leu Asp Glu Asn147514801485ACC GCC AAG GAG GTG ACC AAG CAG CTG AAC GAC ACC ACC GGC AAG TTC 3167Thr Ala Lys Glu Val Thr Lys Gln Leu Asn Asp Thr Thr Gly Lys Phe149014951500AAG GAC GTG AGC CAC CTG TAC GAC GTG AAG CTG ACC CCC AAG ATG AAC 3215Lys Asp Val Ser His Leu Tyr Asp Val Lys Leu Thr Pro Lys Met Asn150515101515GTG ACC ATC AAG CTG AGC ATC CTG TAC GAC AAC GCC GAG AGC AAC GAC 3263Val Thr Ile Lys Leu Ser Ile Leu Tyr Asp Asn Ala Glu Ser Asn Asp1520152515301535AAC AGC ATC GGC AAG TGG ACC AAC ACC AAC ATC GTG AGC GGC GGC AAC 3311Asn Ser Ile Gly Lys Trp Thr Asn Thr Asn Ile Val Ser Gly Gly Asn154015451550AAC GGC AAG AAG CAG TAC AGC AGC AAC AAC CCC GAC GCC AAC CTG ACC 3359Asn Gly Lys Lys Gln Tyr Ser Ser Asn Asn Pro Asp Ala Asn Leu Thr155515601565CTG AAC ACC GAC GCC CAG GAG AAG CTG AAC AAG AAC CGC GAC TAC TAC 3407Leu Asn Thr Asp Ala Gln Glu Lys Leu Asn Lys Asn Arg Asp Tyr Tyr1570 1575 1580ATC AGC CTG TAC ATG AAG AGC GAG AAG AAC ACC CAG TGC GAG ATC ACC 3455Ile Ser Leu Tyr Met Lys Ser Glu Lys Asn Thr Gln Cys Glu Ile Thr158515901595ATC GAC GGC GAG ATA TAC CCC ATC ACC ACC AAG ACC GTG AAC GTG AAC 3503Ile Asp Gly Glu Ile Tyr Pro Ile Thr Thr Lys Thr Val Asn Val Asn1600160516101615AAG GAC AAC TAC AAG CGC CTG GAC ATC ATC GCC CAC AAC ATC AAG AGC 3551Lys Asp Asn Tyr Lys Arg Leu Asp Ile Ile Ala His Asn Ile Lys Ser162016251630AAC CCC ATC AGC AGC CTG CAC ATC AAG ACC AAC GAC GAG ATC ACC CTG 3599Asn Pro Ile Ser Ser Leu His Ile Lys Thr Asn Asp Glu Ile Thr Leu163516401645TIC TGG GAC GAC ATA TCG ATT ACC GAC GTC GCC AGC ATC AAG CCC GAG 3647Phe Trp Asp Asp Ile Ser Ile Thr Asp Val Ala Ser Ile Lys Pro Glu165016551660AAC CTG ACC GAC AGC GAG ATC AAG CAG ATA TAC AGT CGC TAC GGC ATC 3695Asn Leu Thr Asp Ser Glu Ile Lys Gln Ile Tyr Ser Arg Tyr Gly Ile166516701675AAG CTG GAG GAC GGC ATC CTG ATC GAC AAG AAA GGC GGC ATC CAC TAC 3743Lys Leu Glu Asp Gly Ile Leu Ile Asp Lys Lys Gly Gly Ile His Tyr1680168516901695GGC GAG TTC ATC AAC GAG GCC AGC TTC AAC ATC GAG CCC CTG CAG AAC 3791Gly Glu Phe Ile Asn Glu Ala Ser Phe Asn Ile Glu Pro Leu Gln Asn170017051710TAC GTG ACC AAG TAC GAG GTG ACC TAC AGC AGC GAG CTG GGC CCC AAC 3839Tyr Val Thr Lys Tyr Glu Val Thr Tyr Ser Ser Glu Leu Gly Pro Asn171517201725GTG AGC GAC ACC CTG GAG AGC GAC AAG ATT TAC AAG GAC GGC ACC ATC 3887Val Ser Asp Thr Leu Glu Ser Asp Lys Ile Tyr Lys Asp Gly Thr Ile173017351740AAG TTC GAC TTC ACC AAG TAC AGC AAG AAC GAG CAG GGC CTG TTC TAC 3935Lys Phe Asp Phe Thr Lys Tyr Ser Lys Asn Glu Gln Gly Leu Phe Tyr174517501755GAC AGC GGC CTG AAC TGG GAC TTC AAG ATC AAC GCC ATC ACC TAC GAC 3983Asp Ser Gly Leu Asn Trp Asp Phe Lys Ile Asn Ala Ile Thr Tyr Asp1760176517701775GGC AAG GAG ATG AAC GTG TTC CAC CGC TAC AAC AAG TAGATCTGAG4029Gly Lys Glu Met Asn Val Phe His Arg Tyr Asn Lys17801785CT4031(2)关于SEQ ID NO:50的信息(ⅰ)序列特征
(A)长度1338个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:50:Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln1 5 10 15Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu20 25 30Leu Asn Asn Glu Val Ile Lys Ala Glu Gln Leu Asn Ile Asn Ser Gln35 40 45Ser Lys Tyr Thr Asn Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu50 55 60Asp Phe Lys Glu Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys65 70 75 80Glu Lys Glu Trp Lys Leu Thr Ala Thr Glu Lys Gly Lys Met Asn Asn85 90 95Phe Leu Asp Asn Lys Asn Asp Ile Lys Thr Asn Tyr Lys Glu Ile Thr100 105 110Phe Ser Ile Ala Gly Ser Phe Glu Asp Glu Ile Lys Asp Leu Lys Glu115 120 125Ile Asp Lys Met Phe Asp Lys Thr Asn Leu Ser Asn Ser Ile Ile Thr130 135 140Tyr Lys Asn Val Glu Pro Thr Thr Ile Gly Phe Asn Lys Ser Leu Thr145 150 155 160Glu Gly Asn Thr Ile Asn Ser Asp Ala Met Ala Gln Phe Lys Glu Gln165 170 175Phe Leu Asp Arg Asp Ile Lys Phe Asp Ser Tyr Leu Asp Thr His Leu180 185 190Thr Ala Gln Gln Val Ser Ser Lys Glu Arg Val Ile Leu Lys Val Thr195 200 205Val Pro Ser Gly Lys Gly Ser Thr Thr Pro Thr Lys Ala Gly Val Ile210 215 220Leu Asn Asn Ser Glu Tyr Lys Met Leu Ile Asp Asn Gly Tyr Met Val225 230 235 240His Val Asp Lys Val Ser Lys Val Val Lys Lys Gly Val Glu Cys Leu245 250 255Gln Ile Glu Gly Thr Leu Lys Lys Ser Leu Asp Phe Lys Asn Asp Ile260 265 270Asn Ala Glu Ala His Ser Trp Gly Met Lys Asn Tyr Glu Glu Trp Ala275 280 285Lys Asp Leu Thr Asp Ser Gln Arg Glu Ala Leu Asp Gly Tyr Ala Arg290 295 300Gln Asp Tyr Lys Glu Ile Asn Asn Tyr Leu Arg Asn Gln Gly Gly Ser305 310 315 320Gly Asn Glu Lys Leu Asp Ala Gln Ile Lys Asn Ile Ser Asp Ala Leu325 330 335Gly Lys Lys Pro Ile Pro Glu Asn Ile Thr Val Tyr Arg Trp Cys Gly340 345 350Met Pro Glu Phe Gly Tyr Gln Ile Ser Asp Pro Leu Pro Ser Leu Lys355 360 365Asp Phe Glu Glu Gln Phe Leu Asn Thr Ile Lys Glu Asp Lys Gly Tyr370 375 380Met Ser Thr Ser Leu Ser Ser Glu Arg Leu Ala Ala Phe Gly Ser Arg385 390 395 400Lys Ile Ile Leu Arg Leu Gln Val Pro Lys Gly Ser Thr Gly Ala Tyr405 410 415Leu Ser Ala Ile Gly Gly Phe Ala Ser Glu Lys Glu Ile Leu Leu Asp420 425 430Lys Asp Ser Lys Tyr His Ile Asp Lys Val Thr Glu Val Ile Ile Lys435 440 445Gly Val Lys Arg Tyr Val Val Asp Ala Thr Leu Leu Thr Asn Ser Arg450 455 460Gly Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro Thr Pro465 470 475 480Ser Asp Ile Gly Ser Thr Met Lys Thr Asn Gln Ile Ser Thr Thr Gln485 490 495Lys Asn Gln Gln Lys Glu Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr500 505 510Phe Lys Gly Lys Asp Phe Ser Asn Leu Thr Met Phe Ala Pro Thr Arg515 520 525Asp Ser Thr Leu Ile Tyr Asp Gln Gln Thr Ala Asn Lys Leu Leu Asp530 535 540Lys Lys Gln Gln Glu Tyr Gln Ser Ile Arg Trp Ile Gly Leu Ile Gln545 550 555 560Ser Lys Glu Thr Gly Asp Phe Thr Phe Asn Leu Ser Glu Asp Glu Gln565 570 575Ala Ile Ile Glu Ile Asn Gly Lys Ile Ile Ser Asn Lys Gly Lys Glu580 585 590Lys Gln Val Val His Leu Glu Lys Gly Lys Leu Val Pro Ile Lys Ile595 600 605Glu Tyr Gln Ser Asp Thr Lys Phe Asn Ile Asp Ser Lys Thr Phe Lys610 615 620Glu Leu Lys Leu Phe Lys Ile Asp Ser Gln Asn Gln Pro Gln Gln Val625 630 635 640Gln Gln Asp Glu Leu Arg Asn Pro Glu Phe Asn Lys Lys Glu Ser Gln645 650 655Glu Phe Leu Ala Lys Pro Ser Lys Ile Asn Leu Phe Thr Gln Gln Met660 665 670Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser Ile Pro675 680 685Asp Leu Trp Glu Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile Ala Val690 695 700Lys Trp Asp Asp Ser Leu Ala Ser Lys Gly Tyr Thr Lys Phe Val Ser705 710 715 720Asn Pro Leu Glu Ser His Thr Val Gly Asp Pro Tyr Thr Asp Tyr Glu725 730 735Lys Ala Ala Arg Asp Leu Asp Leu Ser Asn Ala Lys Glu Thr Phe Asn740 745 750Pro Leu Val Ala Ala Phe Pro Ser Val Asn Val Ser Met Glu Lys Val755 760 765Ile Leu Ser Pro Asn Glu Asn Leu Ser Asn Ser Val Glu Ser His Ser770 775 780Ser Thr Asn Trp Ser Tyr Thr Asn Thr Glu Gly Ala Ser Val Glu Ala785 790 795 800Gly Ile Gly Pro Lys Gly Ile Ser Phe Gly Val Ser Val Asn Tyr Gln805 810 815His Ser Glu Thr Val Ala Gln Glu Trp Gly Thr Ser Thr Gly Asn Thr820 825 830Ser Gln Phe Asn Thr Ala Ser Ala Gly Tyr Leu Asn Ala Asn Val Arg835 840 845Tyr Asn Asn Val Gly Thr Gly Ala Ile Tyr Asp Val Lys Pro Thr Thr850 855 860Ser Phe Val Leu Asn Asn Asp Thr Ile Ala Thr Ile Thr Ala Lys Ser865 870 875 880Asn Ser Thr Ala Leu Asn Ile Ser Pro Gly Glu Ser Tyr Pro Lys Lys885 890 895Gly Gln Asn Gly Ile Ala Ile Thr Ser Met Asp Asp Phe Asn Ser His900 905 910Pro Ile Thr Leu Asn Lys Lys Gln Val Asp Asn Leu Leu Asn Asn Lys915 920 925Pro Met Met Leu Glu Thr Asn Gln Thr Asp Gly Val Tyr Lys Ile Lys930 935 940Asp Thr His Gly Asn Ile Val Thr Gly Gly Glu Trp Asn Gly Val Ile945 950 955 960Gln Gln Ile Lys Ala Lys Thr Ala Ser Ile Ile Val Asp Asp Gly Glu965 970 975Arg Val Ala Glu Lys Arg Val Ala Ala Lys Asp Tyr Glu Asn Pro Glu980 985 990Asp Lys Thr Pro Ser Leu Thr Leu Lys Asp Ala Leu Lys Leu Ser Tyr995 10001005Pro Asp Glu Ile Lys Glu Ile Glu Gly Leu Leu Tyr Tyr Lys Asn Lys101010151020Pro Ile Tyr Glu Ser Ser Val Met Thr Tyr Leu Asp Glu Asn Thr Ala1025103010351040Lys Glu Val Thr Lys Gln Leu Asn Asp Thr Thr Gly Lys Phe Lys Asp104510501055Val Ser His Leu Tyr Asp Val Lys Leu Thr Pro Lys Met Asn Val Thr106010651070Ile Lys Leu Ser Ile Leu Tyr Asp Asn Ala Glu Ser Asn Asp Asn Ser107510801085Ile Gly Lys Trp Thr Asn Thr Asn Ile Val Ser Gly Gly Asn Asn Gly109010951100Lys Lys Gln Tyr Ser Ser Asn Asn Pro Asp Ala Asn Leu Thr Leu Asn1105111011151120Thr Asp Ala Gln Glu Lys Leu Asn Lys Asn Arg Asp Tyr Tyr Ile Ser112511301135Leu Tyr Met Lys Ser Glu Lys Asn Thr Gln Cys Glu Ile Thr Ile Asp114011451150Gly Glu Ile Tyr Pro Ile Thr Thr Lys Thr Val Asn Val Asn Lys Asp115511601165Asn Tyr Lys Arg Leu Asp Ile Ile Ala His Asn Ile Lys Ser Asn Pro117011751180Ile Ser Ser Leu His Ile Lys Thr Asn Asp Glu Ile Thr Leu Phe Trp1185119011951200Asp Asp Ile Ser Ile Thr Asp Val Ala Ser Ile Lys Pro Glu Asn Leu120512101215Thr Asp Ser Glu Ile Lys Gln Ile Tyr Ser Arg Tyr Gly Ile Lys Leu122012251230Glu Asp Gly Ile Leu Ile Asp Lys Lys Gly Gly Ile His Tyr Gly Glu123512401245Phe Ile Asn Glu Ala Ser Phe Asn Ile Glu Pro Leu Gln Asn Tyr Val125012551260Thr Lys Tyr Glu Val Thr Tyr Ser Ser Glu Leu Gly Pro Asn Val Ser1265127012751280Asp Thr Leu Glu Ser Asp Lys Ile Tyr Lys Asp Gly Thr Ile Lys Phe128512901295Asp Phe Thr Lys Tyr Ser Lys Asn Glu Gln Gly Leu Phe Tyr Asp Ser130013051310Gly Leu Asn Trp Asp Phe Lys Ile Asn Ala Ile Thr Tyr Asp Gly Lys131513201325Glu Met Asn Val Phe His Arg Tyr Asn Lys13301335(2)关于SEQ ID NO:51的信息(ⅰ)序列特征(A)长度2444个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(基因组)(ⅲ)假拟否(ⅸ)特征
(A)名称/键CDS(B)位置17..2444(D)其它信息/产品=“3A(a)合成的天然的融合”(ⅹⅰ)序列描述SEQ ID NO:51:GGATCCACCA ATGAAC ATG AAC AAG AAC AAC ACC AAG CTG AGC ACC CGC 49Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg1 5 10GCC CTG CCG AGC TTC ATC GAC TAC TTC AAC GGC ATC TAC GGC TTC GCC 97Ala Leu Pro Ser Phe Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala15 20 25ACC GGC ATC AAG GAC ATC ATG AAC ATG ATC TTC AAG ACC GAC ACC GGC145Thr Gly Ile Lys Asp Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly30 35 40GGC GAC CTG ACC CTG GAC GAG ATC CTG AAG AAC CAG CAG CTG CTG AAC193Gly Asp Leu Thr Leu Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn45 50 55GAC ATC AGC GGC AAG CTG GAC GGC GTG AAC GGC AGC CTG AAC GAC CTG241Asp Ile Ser Gly Lys Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu60 65 70 75ATC GCC CAG GGC AAC CTG AAC ACC GAG CTG AGC AAG GAG ATC CTT AAG289Ile Ala Gln Gly Asn Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys80 85 90ATC GCC AAC GAG CAG AAC CAG GTG CTG AAC GAC GTG AAC AAC AAG CTG337Ile Ala Asn Glu Gln Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu95 100 105GAC GCC ATC AAC ACC ATG CTG CGC GTG TAC CTG CCG AAG ATC ACC AGC385Asp Ala Ile Asn Thr Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser110 115 120ATG CTG AGC GAC GTG ATG AAG CAG AAC TAC GCC CTG AGC CTG CAG ATC433Met Leu Ser Asp Val Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile125 130 135GAG TAC CTG AGC AAG CAG CTG CAG GAG ATC AGC GAC AAG CTG GAC ATC481Glu Tyr Leu Ser Lys Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile140 145 150 155ATC AAC GTG AAC GTC CTG ATC AAC AGC ACC CTG ACC GAG ATC ACC CCG529Ile Asn Val Asn Val Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro160 165 170GCC TAC CAG CGC ATC AAG TAC GTG AAC GAG AAG TTC GAA GAG CTG ACC577Ala Tyr Gln Arg Ile Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr175 180 185TTC GCC ACC GAG ACC AGC AGC AAG GTG AAG AAG GAC GGC AGC CCG GCC625Phe Ala Thr Glu Thr Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala190 195 200GAC ATC CTG GAC GAG CTG ACC GAG CTG ACC GAG CTG GCC AAG AGC GTG673Asp Ile Leu Asp Glu Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val205 210 215ACC AAG AAC GAC GTG GAC GGC TTC GAG TTC TAC CTG AAC ACC TTC CAC721Thr Lys Asn Asp Val Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His220 225 230 235GAC GTG ATG GTG GGC AAC AAC CTG TTC GGC CGC AGC GCC CTG AAG ACC769Asp Val Met Val Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr240 245 250GCC ACC GAG CTG ATC ACC AAG GAG AAC GTG AAG ACC AGC GGC AGC GAG817Ala Ser Glu Leu Ile Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu255 260 265GTG GGC AAC GTG TAC AAC TTC CTG ATC GTG CTG ACC GCC CTG CAG GCC865Val Gly Asn Val Tyr Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala270 275 280CAG GCC TTC CTG ACC CTG ACC ACC TGT CGC AAG CTG CTG GGC CTG GCC913Gln Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala285 290 295GAC ATC GAC TAC ACC AGC ATC ATG AAC GAG CAC TTG AAC AAG GAG AAG961Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys300 305 310 315GAG GAG TTC CGC GTG AAC ATC CTG CCG ACC CTG AGC AAC ACC TTC AGC 1009Glu Glu Phe Arg Val Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser320 325 330AAC CCG AAC TAC GCC AAG GTG AAG GGC AGC GAC GAG GAC GCC AAG ATG 1057Asn Pro Asn Tyr Ala Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met335 340 345ATC GTG GAG GCT AAG CCG GGC CAC GCG TTG ATC GGC TTC GAG ATC AGC 1105Ile Val Glu Ala Lys Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser350 355 360AAC GAC AGC ATC ACC GTG CTG AAG GTG TAC GAG GCC AAG CTG AAG CAG 1153Asn Asp Ser Ile Thr Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln365 370 375AAC TAC CAG GTG GAC AAG GAC AGC TTG AGC GAG GTG ATC TAC GGC GAC 1201Asn Tyr Gln Val Asp Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp380 385 390 395ATG GAC AAG CTG CTG TGT CCG GAC CAG AGC GAG CAA ATC TAC TAC ACC 1249Met Asp Lys Leu Leu Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr400 405 410AAC AAC ATC GTG TTC CCG AAC GAG TAC GTG ATC ACC AAG ATC GAC TTC1297Asn Asn Ile Val Phe Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe415 420 425ACC AAG AAG ATG AAG ACC CTG CGC TAC GAG GTG ACC GCC AAC TTC TAC1345Thr Lys Lys Met Lys Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr430 435 440GAC AGC AGC ACC GGC GAG ATC GAC CTG AAC AAG AAG AAG GTG GAG AGC1393Asp Ser Ser Thr Gly Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser445 450 455AGC GAG GCC GAG TAC CGC ACC CTG AGC GCG AAC GAC GAC GGC GTC TAC1441Ser Glu Ala Glu Tyr Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr460 465 470 475ATG CCA CTG GGC GTG ATC AGC GAG ACC TTC CTG ACC CCG ATC AAC GGC1489Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly480 485 490TTT GGC CTG CAG GCC GAC GAG AAC AGC CGC CTG ATC ACC CTG ACC TGT1537Phe Gly Leu Gln Ala Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys495 500 505AAG AGC TAC CTG CGC GAG CTG CTG CTA GCC ACC GAC CTG AGC AAC AAG1585Lys Ser Tyr Leu Arg Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys510 515 520GAG ACC AAG CTG ATC GTG CCA CCG AGC GGC TTC ATC AGC AAC ATC GTG1633Glu Thr Lys Leu Ile Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val525 530 535GAG AAC GGC AGC ATC GAG GAG GAC AAC CTG GAG CCG TGG AAG GCC AAC1681Glu Asn Gly Ser Ile Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn540 545 550 555AAC AAG AAC GCC TAC GTG GAC CAC ACC GGC GGC GTG AAC GGC ACC AAG1729Asn Lys Asn Ala Tyr Val Asp His Thr Gly Gly Val Asn Gly Thr Lys560 565 570GCC CTG TAC GTG CAC AAG GAC GGC GGC ATC AGC CAG TIC ATC GGC GAC1777Ala Leu Tyr Val His Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp575 580 585AAG CTG AAG CCG AAG ACC GAG TAC GTG ATC CAG TAC ACC GTG AAG GGC1825Lys Leu Lys Pro Lys Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly590 595 600AAG CCA TCG ATT CAC CTG AAG GAC GAG AAC ACC GGC TAC ATC CAC TAC1873Lys Pro Ser Ile His Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr605 610 615GAG GAC ACC AAC AAC AAC CTG GAG GAC TAC CAG ACC ATC AAC AAG CGC1921Glu Asp Thr Asn Asn Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg620 625 630 635TTC ACC ACC GGC ACC GAC CTG AAG GGC GTG TAC CTG ATC CTG AAG AGC 1969Phe Thr Thr Gly Thr Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser640 645 650CAG AAC GGC GAC GAG GCC TGG GGC GAC AAC TTC ATC ATC CTG GAG ATC 2017Gln Asn Gly Asp Glu Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile655 660 665AGC CCG AGC GAG AAG CTG CTG AGC CCG GAG CTG ATC AAC ACC AAC AAC 2065Ser Pro Ser Glu Lys Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn670 675 680TGG ACC AGC ACC GGC AGC ACC AAC ATC AGC GGC AAC ACC CTG ACC CTG 2113Trp Thr Ser Thr Gly Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu685 690 695TAC CAG GGC GGC CGG GGG ATT CTA AAA CAA AAC CTT CAA TTA GAT AGT 2161Tyr Gln Gly Gly Arg Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser700 705 710 715TTT TCA ACT TAT AGA GTG TAT TTT TCT GTG TCC GGA GAT GCT AAT GTA 2209Phe Ser Thr Tyr Arg Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val720 725 730AGG ATT AGA AAT TCT AGG GAA GTG TTA TTT GAA AAA AGA TAT ATG AGC 2257Arg Ile Arg Asn Ser Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser735 740 745GGT GCT AAA GAT GTT TCT GAA ATG TTC ACT ACA AAA TTT GAG AAA GAT 2305Gly Ala Lys Asp Val Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp750 755 760AAC TTT TAT ATA GAG CTT TCT CAA GGG AAT AAT TTA TAT GGT GGT CCT 2353Asn Phe Tyr Ile Glu Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro765 770 775ATT GTA CAT TTT TAC GAT GTC TCT ATT AAG NAA GAT CGG GAT CTA ATA 2401Ile Val His Phe Tyr Asp Val Ser Ile Lys Xaa Asp Arg Asp Leu Ile780 785 790 795TTA ACA GTT TTT AAA AGC NAA TTC TTG TAT AAT GTC CTT GAT T 2444Leu Thr Val Phe Lys Ser Xaa Phe Leu Tyr Asn Val Leu Asp800 805(2)关于SEQ ID NO:52的信息(ⅰ)序列特征(A)长度809个氨基酸(B)类型氨基酸(D)拓扑结构线型(ⅱ)分子类型蛋白质(ⅹⅰ)序列描述SEQ ID NO:52:Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe1 5 10 15Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp20 25 30Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu35 40 45Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys50 55 60Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn65 70 75 80Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln85 90 95Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr100 105 110Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val115 120 125Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys130 135 140Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val145 150 155 160Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile165 170 175Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr180 185 190Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu Asp Glu195 200 205Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val210 215 220Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly225 230 235 240Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile245 250 255Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr260 265 270Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Gln Ala Phe Leu Thr275 280 285Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr290 295 300Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val305 310 315 320Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala325 330 335Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys340 345 350Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr355 360 365Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp370 375 380Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu385 390 395 400Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe405 410 415Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys420 425 430Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly435 440 445Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr450 455 460Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val465 470 475 480Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala485 490 495Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg500 505 510Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile515 520 525Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Ser Ile530 535 540Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr545 550 555 560Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His565 570 575Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys580 585 590Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His595 600 605Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn610 615 620Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr Gly Thr625 630 635 640Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu645 650 655Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys660 665 670Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly675 680 685Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg690 695 700Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg705 710 715 720Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser725 730 735Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val740 745 750Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr Ile Glu755 760 765Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His Phe Tyr770 775 780Asp Val Ser Ile Lys Xaa Asp Arg Asp Leu Ile Leu Thr Val Phe Lys785 790 795 800Ser Xaa Phe Leu Tyr Asn Val Leu Asp805(2)关于SEQ ID NO:53的信息(ⅰ)序列特征(A)长度3474个碱基对
(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(ⅲ)假拟否(ⅳ)反义否(ⅵ)原始来源(A)生物纯化的玉米最佳化的合成BT CryⅠA(b)基因(ⅹⅰ)序列描述SEQ ID NO:53:ATGGACAACA ACCCCAACAT CAACGAGTGC ATCCCCTACA ACTGCCTGAG CAACCCCGAG60GTGGAGGTGC TGGGCGGCGA GCGCATCGAG ACCGGCTACA CCCCCATCGA CATCAGCCTG120AGCCTGACCC AGTTCCTGCT GAGCGAGTTC GTGCCCGGCG CCGGCTTCGT GCTGGGCCTG180GTGGACATCA TCTGGGGCAT CTTCGGCCCC AGCCAGTGGG ACGCCTTCCT GGTGCAGATC240GAGCAGCTGA TCAACCAGCG CATCGAGGAG TTCGCCCGCA ACCAGGCCAT CAGCCGCCTG300GAGGGCCTGA GCAACCTGTA CCAGATCTAC GCCGAGAGCT TCCGCGAGTG GGAGGCCGAC360CCCACCAACC CCGCCCTGCG CGAGGAGATG CGCATCCAGT TCAACGACAT GAACAGCGCC420CTGACCACCG CCATCCCCCT GTTCGCCGTG CAGAACTACC AGGTGCCCCT GCTGAGCGTG480TACGTGCAGG CCGCCAACCT GCACCTGAGC GTGCTGCGCG ACGTGAGCGT GTTCGGCCAG540CGCTGGGGCT TCGACGCCGC CACCATCAAC AGCCGCTACA ACGACCTGAC CCGCCTGATC600GGCAACTACA CCGACCACGC CGTGCGCTGG TACAACACCG GCCTGGAGCG CGTGTGGGGC660CCCGACAGCC GCGACTGGAT CCGCTACAAC CAGTTCCGCC GCGAGCTGAC CCTGACCGTG720CTGGACATCG TGAGCCTGTT CCCCAACTAC GACAGCCGCA CCTACCCCAT CCGCACCGTG780AGCCAGCTGA CCCGCGAGAT CTACACCAAC CCCGTGCTGG AGAACTTCGA CGGCAGCTTC840CGCGGCAGCG CCCAGGGCAT CGAGGGCAGC ATCCGCAGCC CCCACCTGAT GGACATCCTG900AACAGCATCA CCATCTACAC CGACGCCCAC CGCGGCGAGT ACTACTGGAG CGGCCACCAG960ATCATGGCCA GCCCCGTGGG CTTCAGCGGC CCCGAGTTCA CCTTCCCCCT GTACGGCACC1020ATGGGCAACG CCGCCCCCCA GCAGCGCATC GTGGCCCAGC TGGGCCAGGG CGTGTACCGC1080ACCCTGAGCA GCACCCTGTA CCGCCGCCCC TTCAACATCG GCATCAACAA CCAGCAGCTG1140AGCGTGCTGG ACGGCACCGA GTTCGCCTAC GGCACCAGCA GCAACCTGCC CAGCGCCGTG1200TACCGCAAGA GCGGCACCGT GGACAGCCTG GACGAGATCC CCCCCCAGAA CAACAACGTG1260CCCCCCCGCC AGGGCTTCAG CCACCGCCTG AGCCACGTGA GCATGTTCCG CAGCGGCTTC1320AGCAACAGCA GCGTGAGCAT CATCCGCGCC CCCATGTTCA GCTGGATCCA CCGCAGCGCC1380GAGTTCAACA ACATCATCCC CAGCAGCCAG ATCACCCAGA TCCCCCTGAC CAAGAGCACC1440AACCTGGGCA GCGGCACCAG CGTGGTGAAG GGCCCCGGCT TCACCGGCGG CGACATCCTG1500CGCCGCACCA GCCCCGGCCA GATCAGCACC CTGCGCGTGA ACATCACCGC CCCCCTGAGC1560CAGCGCTACC GCGTGCGCAT CCGCTACGCC AGCACCACCA ACCTGCAGTT CCACACCAGC1620ATCGACGGCC GCCCCATCAA CCAGGGCAAC TTCAGCGCCA CCATGAGCAG CGGCAGCAAC1680CTGCAGAGCG GCAGCTTCCG CACCGTGGGC TTCACCACCC CCTTCAACTT CAGCAACGGC1740AGCAGCGTGT TCACCCTGAG CGCCCACGTG TTCAACAGCG GCAACGAGGT GTACATCGAC1800CGCATCGAGT TCGTGCCCGC CGAGGTGACC TTCGAGGCCG AGTACGACCT GGAGCGCGCC1860CAGAAGGCCG TGAACGAGCT GTTCACCAGC AGCAACCAGA TCGGCCTGAA GACCGACGTG1920ACCGACTACC ACATCGACCA GGTGAGCAAC CTGGTGGAGT GCCTGAGCGA CGAGTTCTGC1980CTGGACGAGA AGAAGGAGCT GAGCGAGAAG GTGAAGCACG CCAAGCGCCT GAGCGACGAG2040CGCAACCTGC TGCAGGACCC CAACTTCCGC GGCATCAACC GCCAGCTGGA CCGCGGCTGG2100CGCGGCAGCA CCGACATCAC CATCCAGGGC GGCGACGACG TGTTCAAGGA GAACTACGTG2160ACCCTGCTGG GCACCTTCGA CGAGTGCTAC CCCACCTACC TGTACCAGAA GATCGACGAG2220AGCAAGCTGA AGGCCTACAC CCGCTACCAG CTGCGCGGCT ACATCGAGGA CAGCCAGGAC2280CTGGAGATCT ACCTGATCCG CTACAACGCC AAGCACGAGA CCGTGAACGT GCCCGGCACC2340GGCAGCCTGT GGCCCCTGAG CGCCCCCAGC CCCATCGGCA AGTGCGCCCA CCACAGCCAC2400CACTTCAGCC TGGACATCGA CGTGGGCTGC ACCGACCTGA ACGAGGACCT GGGCGTGTGG2460GTGATCTTCA AGATCAAGAC CCAGGACGGC CACGCCCGCC TGGGCAACCT GGAGTTCCTG2520GAGGAGAAGC CCCTGGTGGG CGAGGCCCTG GCCCGCGTGA AGCGCGCCGA GAAGAAGTGG2580CGCGACAAGC GCGAGAAGCT GGAGTGGGAG ACCAACATCG TGTACAAGGA GGCCAAGGAG2640AGCGTGGACG CCCTGTTCGT GAACAGCCAG TACGACCGCC TGCAGGCCGA CACCAACATC2700GCCATGATCC ACGCCGCCGA CAAGCGCGTG CACAGCATCC GCGAGGCCTA CCTGCCCGAG2760CTGAGCGTGA TCCCCGGCGT GAACGCCGCC ATCTTCGAGG AGCTGGAGGG CCGCATCTTC2820ACCGCCTTCA GCCTGTACGA CGCCCGCAAC GTGATCAAGA ACGGCGACTT CAACAACGGC2880CTGAGCTGCT GGAACGTGAA GGGCCACGTG GACGTGGAGG AGCAGAACAA CCACCGCAGC2940GTGCTGGTGG TGCCCGAGTG GGAGGCCGAG GTGAGCCAGG AGGTGCGCGT GTGCCCCGGC3000CGCGGCTACA TCCTGCGCGT GACCGCCTAC AAGGAGGGCT ACGGCGAGGG CTGCGTGACC3060ATCCACGAGA TCGAGAACAA CACCGACGAG CTGAAGTTCA GCAACTGCGT GGAGGAGGAG3120GTGTACCCCA ACAACACCGT GACCTGCAAC GACTACACCG CCACCCAGGA GGAGTACGAG3180GGCACCTACA CCAGCCGCAA CCGCGGCTAC GACGGCGCCT ACGAGAGCAA CAGCAGCGTG3240CCCGCCGACT ACGCCAGCGC CTACGAGGAG AAGGCCTACA CCGACGGCCG CCGCGACAAC3300CCCTGCGAGA GCAACCGCGG CTACGGCGAC TACACCCCCC TGCCCGCCGG CTACGTGACC3360AAGGAGCTGG AGTACTTCCC CGAGACCGAC AAGGTGTGGA TCGAGATCGG CGAGACCGAG3420GGCACCTTCA TCGTGGACAG CGTGGAGCTG CTGCTGATGG AGGAGTAGTA CATG3474(2)关于SEQ ID NO:54的信息(ⅰ)序列特征(A)长度3508个核苷酸(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(ⅳ)反义否(ⅵ)原始来源(A)生物合成的玉米最佳化的全长BT CryⅠA(b)基因(ⅹⅰ)序列描述SEQ ID NO:54:GATCCAACAA TGGACAACAA CCCCAACATC AACGAGTGCA TCCCCTACAA CTGCCTGAGC60AACCCCGAGG TGGAGGTGCT GGGCGGCGAG CGCATCGAGA CCGGCTACAC CCCCATCGAC120ATCAGCCTGA GCCTGACCCA GTTCCTGCTG AGCGAGTTCG TGCCCGGCGC CGGCTTCGTG180CTGGGCCTGG TGGACATCAT CTGGGGCATC TTCGGCCCCA GCCAGTGGGA CGCCTTCCTG240GTGCAGATCG AGCAGCTGAT CAACCAGCGC ATCGAGGAGT TCGCCCGCAA CCAGGCCATC300AGCCGCCTGG AGGGCCTGAG CAACCTGTAC CAAATCTACG CCGAGAGCTT CCGCGAGTGG360GAGGCCGACC CCACCAACCC CGCCCTGCGC GAGGAGATGC GCATCCAGTT CAACGACATG420AACAGCGCCC TGACCACCGC CATCCCCCTG TTCGCCGTGC AGAACTACCA GGTGCCCCTG480CTGAGCGTGT ACGTGCAGGC CGCCAACCTG CACCTGAGCG TGCTGCGCGA CGTCAGCGTG540TTCGGCCAGC GCTGGGGCTT CGACGCCGCC ACCATCAACA GCCGCTACAA CGACCTGACC600CGCCTGATCG GCAACTACAC CGACCACGCC GTGCGCTGGT ACAACACCGG CCTGGAGCGC660GTGTGGGGTC CCGACAGCCG CGACTGGATC AGGTACAACC AGTTCCGCCG CGAGCTGACC720CTGACCGTGC TGGACATCGT GAGCCTGTTC CCCAACTACG ACAGCCGCAC CTACCCCATC780CGCACCGTGA GCCAGCTGAC CCGCGAGATT TACACCAACC CCGTGCTGGA GAACTTCGAC840GGCAGCTTCC GCGGCAGCGC CCAGGGCATC GAGGGCAGCA TCCGCAGCCC CCACCTGATG900GACATCCTGA ACAGCATCAC CATCTACACC GACGCCCACC GCGGCGAGTA CTACTGGAGC960GGCCACCAGA TCATGGCCAG CCCCGTCGGC TTCAGCGGCC CCGAGTTCAC CTTCCCCCTG1020TACGGCACCA TGGGCAACGC TGCACCTCAG CAGCGCATCG TGGCACAGCT GGGCCAGGGA1080GTGTACCGCA CCCTGAGCAG CACCCTGTAC CGTCGACCTT TCAACATCGG CATCAACAAC1140CAGCAGCTGA GCGTGCTGGA CGGCACCGAG TTCGCCTACG GCACCAGCAG CAACCTGCCC1200AGCGCCGTGT ACCGCAAGAG CGGCACCGTG GACAGCCTGG ACGAGATCCC CCCTCAGAAC1260AACAACGTGC CACCTCGACA GGGCTTCAGC CACCGTCTGA GCCACGTGAG CATGTTCCGC1320AGTGGCTTCA GCAACAGCAG CGTGAGCATC ATCCGTGCAC CTATGTTCAG CTGGATTCAC1380CGCAGTGCCG AGTTCAACAA CATCATCCCC AGCAGCCAGA TCACCCAGAT CCCCCTGACC1440AAGAGCACCA ACCTGGGCAG CGGCACCAGC GTGGTGAAGG GCCCCGGCTT CACCGGCGGC1500GACATCCTGC GCCGCACCAG CCCCGGCCAG ATCAGCACCC TGCGCGTGAA CATCACCGCC1560CCCCTGAGCC AGCGCTACCG CGTCCGCATC CGCTACGCCA GCACCACCAA CCTGCAGTTC1620CACACCAGCA TCGACGGCCG CCCCATCAAC CAGGGCAACT TCAGCGCCAC CATGAGCAGC1680GGCAGCAACC TGCAGAGCGG CAGCTTCCGC ACCGTGGGCT TCACCACCCC CTTCAACTTC1740AGCAACGGCA GCAGCGTGTT CACCCTGAGC GCCCACGTGT TCAACAGCGG CAACGAGGTG1800TACATCGACC GCATCGAGTT CGTGCCCGCC GAGGTGACCT TCGAGGCCGA GTACGACCTG1860GAGAGGGCTC AGAAGGCCGT GAACGAGCTG TTCACCAGCA GCAACCAGAT CGGCCTGAAG1920ACCGACGTGA CCGACTACCA CATCGATCAG GTGAGCAACC TGGTGGAGTG CCTGAGCGAC1980GAGTTCTGCC TGGACGAGAA GAAGGAGCTG AGCGAGAAGG TGAAGCACGC CAAGCGCCTG2040AGCGACGAGC GCAACCTGCT GCAGGACCCC AACTTCCGCG GCATCAACCG CCAGCTGGAC2100CGCGGCTGGC GCGGCAGCAC CGACATCACC ATCCAGGGCG GCGACGACGT GTTCAAGGAG2160AACTACGTGA CCCTGCTGGG CACCTTCGAC GAGTGCTACC CCACCTACCT GTACCAGAAG2220ATCGACGAGA GCAAGCTGAA GGCCTACACC CGCTACCAGC TGCGCGGCTA CATCGAGGAC2280AGCCAGGACC TGGAGATCTA CCTGATCCGC TACAACGCCA AGCACGAGAC CGTGAACGTG2340CCCGGCACCG GCAGCCTGTG GCCCCTGAGC GCCCCCAGCC CCATCGGCAA GTGCGCCCAC2400CACAGCCACC ACTTCAGCCT GGACATCGAC GTGGGCTGCA CCGACCTGAA CGAGGACCTG2460GGCGTGTGGG TGATCTTCAA GATCAAGACC CAGGACGGCC ACGCCCGCCT GGGCAACCTG2520GAGTTCCTGG AGGAGAAGCC CCTGGTGGGC GAGGCCCTGG CCCGCGTGAA GCGCGCCGAG2580AAGAAGTGGC GCGACAAGCG CGAGAAGCTG GAGTGGGAGA CCAACATCGT GTACAAGGAG2640GCCAAGGAGA GCGTGGACGC CCTGTTCGTG AACAGCCAGT ACGACCGCCT GCAGGCCGAC2700ACCAACATCG CCATGATCCA CGCCGCCGAC AAGCGCGTGC ACAGCATTCG CGAGGCCTAC2760CTGCCCGAGC TGAGCGTGAT CCCCGGCGTG AACGCCGCCA TCTTCGAGGA GCTGGAGGGC2820CGCATCTTCA CCGCCTTCAG CCTGTACGAC GCCCGCAACG TGATCAAGAA CGGCGACTTC2880AACAACGGCC TGAGCTGCTG GAACGTGAAG GGCCACGTGG ACGTGGAGGA GCAGAACAAC2940CACCGCAGCG TGCTGGTGGT GCCCGAGTGG GAGGCCGAGG TGAGCCAGGA GGTGCGCGTG3000TGCCCCGGCC GCGGCTACAT CCTGCGCGTG ACCGCCTACA AGGAGGGCTA CGGCGAGGGC3060TGCGTGACCA TCCACGAGAT CGAGAACAAC ACCGACGAGC TCAAGTTCAG CAACTGCGTG3120GAGGAGGAGG TGTACCCCAA CAACACCGTG ACCTGCAACG ACTACACCGC CACCCAGGAG3180GAGTACGAGG GCACCTACAC CAGCCGCAAC CGCGGCTACG ACGGCGCCTA CGAGAGCAAC3240AGCAGCGTGC CCGCCGACTA CGCCAGCGCC TACGAGGAGA AGGCCTACAC CGACGGCCGC3300CGCGACAACC CCTGCGAGAG CAACCGCGGC TACGGCGACT ACACCCCCCT GCCCGCCGGC3360TACGTGACCA AGGAGCTGGA GTACTTCCCC GAGACCGACA AGGTGTGGAT CGAGATCGGC3420GAGACCGAGG GCACCTTCAT CGTGGACAGC GTGGAGCTGC TGCTGATGGA GGAGTAGTAC3480ATGTGATAGT ACGTAAGCTC GAGGATCT 3508(2)关于SEQ ID NO:55的信息(ⅰ)序列特征(A)长度1961个碱基对(B)类型核酸(C)链型单链(D)拓扑结构线型(ⅱ)分子类型DNA(ⅲ)假拟否(ⅳ)反义否(ⅵ)原始来源(A)生物截短的合成的玉米最佳化BT CryⅠA(b)基因(ⅹⅰ)序列描述SEQ ID NO:55:GATCCAACAA TGGACAACAA CCCCAACATC AACGAGTGCA TCCCCTACAA CTGCCTGAGC60AACCCCGAGG TGGAGGTGCT GGGCGGCGAG CGCATCGAGA CCGGCTACAC CCCCATCGAC 120ATCAGCCTGA GCCTGACCCA GTTCCTGCTG AGCGAGTTCG TGCCCGGCGC CGGCTTCGTG 180CTGGGCCTGG TGGACATCAT CTGGGGCATC TTCGGCCCCA GCCAGTGGGA CGCCTTCCTG 240GTGCAGATCG AGCAGCTGAT CAACCAGCGC ATCGAGGAGT TCGCCCGCAA CCAGGCCATC 300AGCCGCCTGG AGGGCCTGAG CAACCTGTAC CAAATCTACG CCGAGAGCTT CCGCGAGTGG 360GAGGCCGACC CCACCAACCC CGCCCTGCGC GAGGAGATGC GCATCCAGTT CAACGACATG 420AACAGCGCCC TGACCACCGC CATCCCCCTG TTCGCCGTGC AGAACTACCA GGTGCCCCTG 480CTGAGCGTGT ACGTGCAGGC CGCCAACCTG CACCTGAGCG TGCTGCGCGA CGTCAGCGTG 540TTCGGCCAGC GCTGGGGCTT CGACGCCGCC ACCATCAACA GCCGCTACAA CGACCTGACC 600CGCCTGATCG GCAACTACAC CGACCACGCC GTGCGCTGGT ACAACACCGG CCTGGAGCGC 660GTGTGGGGTC CCGACAGCCG CGACTGGATC AGGTACAACC AGTTCCGCCG CGAGCTGACC 720CTGACCGTGC TGGACATCGT GAGCCTGTTC CCCAACTACG ACAGCCGCAC CTACCCCATC 780CGCACCGTGA GCCAGCTGAC CCGCGAGATT TACACCAACC CCGTGCTGGA GAACTTCGAC 840GGCAGCTTCC GCGGCAGCGC CCAGGGCATC GAGGGCAGCA TCCGCAGCCC CCACCTGATG 900GACATCCTGA ACAGCATCAC CATCTACACC GACGCCCACC GCGGCGAGTA CTACTGGAGC 960GGCCACCAGA TCATGGCCAG CCCCGTCGGC TTCAGCGGCC CCGAGTTCAC CTTCCCCCTG 1020TACGGCACCA TGGGCAACGC TGCACCTCAG CAGCGCATCG TGGCACAGCT GGGCCAGGGA 1080GTGTACCGCA CCCTGAGCAG CACCCTGTAC CGTCGACCTT TCAACATCGG CATCAACAAC 1140CAGCAGCTGA GCGTGCTGGA CGGCACCGAG TTCGCCTACG GCACCAGCAG CAACCTGCCC 1200AGCGCCGTGT ACCGCAAGAG CGGCACCGTG GACAGCCTGG ACGAGATCCC CCCTCAGAAC 1260AACAACGTGC CACCTCGACA GGGCTTCAGC CACCGTCTGA GCCACGTGAG CATGTTCCGC 1320AGTGGCTTCA GCAACAGCAG CGTGAGCATC ATCCGTGCAC CTATGTTCAG CTGGATTCAC 1380CGCAGTGCCG AGTTCAACAA CATCATCCCC AGCAGCCAGA TCACCCAGAT CCCCCTGACC 1440AAGAGCACCA ACCTGGGCAG CGGCACCAGC GTGGTGAAGG GCCCCGGCTT CACCGGCGGC 1500GACATCCTGC GCCGCACCAG CCCCGGCCAG ATCAGCACCC TGCGCGTGAA CATCACCGCC 1560CCCCTGAGCC AGCGCTACCG CGTCCGCATC CGCTACGCCA GCACCACCAA CCTGCAGTTC 1620CACACCAGCA TCGACGGCCG CCCCATCAAC CAGGGCAACT TCAGCGCCAC CATGAGCAGC 1680GGCAGCAACC TGCAGAGCGG CAGCTTCCGC ACCGTGGGCT TCACCACCCC CTTCAACTTC 1740AGCAACGGCA GCAGCGTGTT CACCCTGAGC GCCCACGTGT TCAACAGCGG CAACGAGGTG 1800TACATCGACC GCATCGAGTT CGTGCCCGCC GAGGTGACCT TCGAGGCCGA GTACGACCTG 1860GAGAGGGCTC AGAAGGCCGT GAACGAGCTG TTCACCAGCA GCAACCAGAT CGGCCTGAAG 1920ACCGACGTGA CCGACTACCA CATCGATCAG GTGTAGGAGC T 1961保藏于农业研究机构保藏中心(NRRL),Northern Regional ResearchCenter,1815 North University Street,Peoria,IIIinois 61604,U.S.A。
菌株命名 保藏号 保藏日期蜡状芽孢杆菌AB78NRRLB-210581993年3月18日大肠杆菌pCIB4431NRRLB-189981992年9月21日
权利要求
1．保护植物及其子代免受由Ostrinia furnacalis种类引起的损害的方法，包括直接或间接向植物或植物种子或植物生长区域施用作为活性成份的芽孢杆菌属种类的毒素蛋白。
2．根据权利要求1所述的方法，其中的毒素蛋白是苏云金芽孢杆菌的毒素蛋白。
3．根据权利要求1所述的方法，其中的毒素蛋白是蜡状芽孢杆菌的毒素蛋白。
4．根据权利要求1所述的方法，其中的毒素蛋白是Cry型蛋白，优选地CryⅠ型蛋白，更优选地CryⅠA型蛋白，最优选地CryⅠA(b)蛋白。
5．根据权利要求4所述的方法，其中的毒素蛋白是根据SEQ ID Nos53-55的CryⅠ型蛋白。
6．根据权利要求1所述的方法，其中的毒素蛋白是VIP型蛋白，优选地VIP1型蛋白，如VIP1A(a)蛋白或VIP1A(b)蛋白，或VIP2型蛋白，如VIP2A(a)蛋白或VIP2A(b)蛋白或VIP1型蛋白和VIP2型蛋白的结合。
7．根据权利要求1所述的方法，其中毒素蛋白是VIP3型蛋白，如VIP3A(a)蛋白或VIP3A(b)蛋白。
8．根据权利要求6-7的任意一项所述的方法，其中毒素蛋白是根据SEQ ID Nos.1,2,4-7,17-24,26-32,35,36,39,40,42,43,45,46,49,50,51或52的VIP型蛋白。
9．根据权利要求1所述的方法，其中毒素蛋白以杀昆虫组合物的形式施用给植物。
10．根据权利要求9所述的方法，其中杀昆虫组合物包括微生物。
11．根据权利要求1或9所述的方法，其中毒素蛋白是CryⅠ型蛋白。
12．根据权利要求9所述的方法，其中杀昆虫组合物包含至少一种与适当载体结合的CryⅠ型毒素蛋白或微生物，优选地包含至少一个编码上述毒素蛋白的基因的苏云金芽孢杆菌和/或蜡状芽孢杆菌。
13．根据权利要求12所述的方法，其中微生物是包含至少一个编码毒素蛋白的CryⅠ型毒素基因的天然存在的生物。
14．根据权利要求10所述的方法，其中微生物是包含至少一个Cry型毒素基因，优选地CryⅠ型毒素基因，更优选地CryⅠA型毒素基因和最优选地CryⅠA(b)毒素基因的天然存在的生物。
15．根据权利要求12所述的方法，其中微生物是包含至少一个编码毒素蛋白的以重组形式的CryⅠ型毒素基因的重组生物。
16．根据权利要求10所述的方法，其中微生物是包含至少一个Cry型毒素基因，优选地CryⅠ型毒素基因，更优选地CryⅠA型毒素基因，最优选地CryⅠA(b)毒素基因的重组生物。
17．根据权利要求10所述的方法，其中杀昆虫组合物包含至少一种与适当载体结合的VIP型毒素蛋白或微生物，优选地包含至少一个编码上述毒素蛋白的基因的苏云金芽孢杆菌和/或蜡状芽孢杆菌。
18．根据权利要求10所述的方法，其中微生物是包含至少一个编码毒素蛋白的VIP型蛋白基因，优选地VIP1A(a)蛋白或VIP1A(b)蛋白基因或VIP2型蛋白基因如VIP2A(a)蛋白或VIP2A(b)蛋白基因的天然存在的生物。
19．根据权利要求10所述的方法，其中微生物是包含至少一个编码毒素蛋白的VIP3型蛋白基因如VIP3A(a)蛋白或VIP3A(b)蛋白基因的天然存在的生物。
20．根据权利要求17-19的任意一项所述的方法，其中毒素蛋白是根据SEQ ID Nos.1,2,4-7,17-24,26-32,35,36,39,40,42,43,45,46,49,50,51或52的Cry型蛋白。
21．根据权利要求11-16的任意一项所述的方法，其中毒素蛋白是根据SEQ ID Nos.53-55的Cry型蛋白。
22．根据权利要求17-21的任意一项所述的，其中微生物是重组生物。
23．根据权利要求1-22所述的方法，其中毒素蛋白通过用编码芽孢杆菌属种类毒素蛋白的毒素基因转化植物，并以足够提供针对Ostriniafurnacalis(亚洲玉米螟)种类的控制的量表达上述毒素蛋白来间接地施用给植物，并在昆虫虫害可能出现的区域种植这样转化的植物。
24．根据权利要求23所述的方法，其中毒素基因编码苏云金芽孢杆菌或蜡状芽孢杆菌的毒素蛋白。
25．根据权利要求23所述的方法，其中毒素基因编码Cry型毒素蛋白，优选地CryⅠ型蛋白，更优选地CryⅠA型蛋白，最优选地CryⅠA(b)蛋白，如在SEQ ID Nos.53-55中提供的。
26．根据权利要求23所述的方法，其中毒素基因编码VIP型蛋白，优选地VIP1型蛋白，如VIP1A(a)蛋白或VIP1A(b)蛋白，或VIP2型蛋白，如VIP2A(a)蛋白或VIP2A(b)蛋白。
27．根据权利要求23所述的方法，其中毒素基因编码VIP3型蛋白，如VIP3A(a)蛋白或VIP3A(b)蛋白。
28．根据权利要求23-27所述的方法，其中毒素基因是合成的基因，其密码子用法通过使用在植物中最优选的密码子最佳化。
29．根据权利要求23和28所述的方法，其中毒素基因编码CryⅠ型蛋白。
30．根据权利要求23-27的任意一项所述的方法，其中毒素基因编码根据SEQ ID Nos.1,2,4-7,17-24,26-32,35,36,39,40,42,43,45,46,50,51或52的VIP型蛋白。
31．根据权利要求1-30的任意一项所述的方法，其中所要保护的植物是谷类植物。
32．根据权利要求31所述的方法，其中所要保护的植物是玉米。
33．根据权利要求1-32的任意一项所述的方法，其中至少一种Cry型毒素蛋白与VIP型毒素蛋白结合以足够提供针对亚洲玉米螟(Ostriniafurnacalis)的控制的量直接或间接施用给植物或植物种子或植物生长区域。
34．根据权利要求1-32的任意一项所述的方法，其中活性成分是CryⅠA(b)蛋白。
35．根据权利要求1-32的任意一项所述的方法，其中活性成分是VIP型蛋白。
36．如在前述权利要求中定义的活性成分的应用，用于在农作物植株中控制Ostrinia furnacalis(亚洲玉米螟)虫害。
38．根据权利要求23-30的任意一项所述的重组微生物或转基因植物的应用，用于在农作物植株中控制Ostrinia furnacalis(亚洲玉米螟)虫害，上述重组微生物或转基因植物包含能在中等严格条件下与编码相应毒素蛋白的Cry型或VIP型基因杂交的DNA分子。
39．包含转基因植物的种子，并与其可靠的使用说明一起，用于在农作物植株中控制Ostrinia furnacalis(亚洲玉米螟)虫害的商品包，上述转基因植物包含编码苏云金芽孢杆菌毒素蛋白的毒素基因并以足够提供针对Ostrinia furnacalis(亚洲玉米螟)种类的控制的量表达上述毒素蛋白。
40．包含转基因植物的种子或微生物，尤其苏云金芽孢杆菌和/或蜡状芽孢杆菌菌株，并与其可靠的使用说明一起，用于在农作物植株中控制Ostrinia furnacalis(亚洲玉米螟)虫害的商品包，上述转基因植物或微生物包含至少一个编码如前权利要求1-30,32和33中定义的毒素蛋白的毒素基因，并且以足够提供针对Ostrinia furnacalis(亚洲玉米螟)种类的控制的量表达上述毒素蛋白。
41．一种农业方法，其中如权利要求23-32的任意一项所述的，使用包含编码芽孢杆菌属种类毒素蛋白的毒素基因并以足够提供针对Ostrinia furnacalis(亚洲玉米螟)种类的控制的量表达上述蛋白的转基因植物或其子代或转基因的种子。
42．根据权利要求18-26的任意一项所述的方法，其中至少一种Cry型毒素蛋白与VIP型毒素蛋白一起以足够提供针对蛀茎夜蛾属虫害的控制的量在所要保护的植物中表达。
43．一种组合物，包含杀昆虫有效量的至少一种Cry型毒素蛋白和VIP型毒素蛋白作为活性成分，并与农学上可接受的载体结合。
全文摘要
本发明提供在农作物植株中控制Ostrinia furnacalis(亚洲玉米暝)的方法,农作物尤其指玉米和其它谷类植物,包括但不限于以下植物种类:玉米,小麦,大麦,黑麦,燕麦,水稻,高梁,黍,和相关作物,饲草,竹和甘蔗。
文档编号A01N63/02GK1221318SQ97195250
公开日1999年6月30日 申请日期1997年5月27日 优先权日1996年6月6日
发明者K·苏宛塔拉顿, B·亨特, W·P·M·尤特德威里根 申请人:诺瓦提斯公司