一种微生物合成植物生长素的方法与流程

本发明属于微生物代谢工程领域，具体涉及改造微生物代谢途径，从单糖直接合成植物生长素的方法。

背景技术：

植物生长素是植物中最为重要的信息素之一，是植物体内普遍存在的内源生长素，对于植物体的生长发育至关重要，具有促进细胞分裂、维管束分化、茎伸长、顶端优势、种子发芽、侧根和不定根形成、根瘤形成、子房壁生长、种子和果实生长、坐果等,在农业生产上具有广阔的应用前景与经济地位。这些植物生长素包括吲哚乙酸、苯乙酸、对羟基苯乙酸等。

目前已商品化的植物生长素均为通过化学法合成。化学法合成生长素需涉及到多个反应步骤导致合成成本较高。通过生物技术手段，实现微生物发酵单糖合成各种生长素，可有效的降低成本。

技术实现要素：

本发明的目的在于提供一种微生物体内合成植物生长素的方法，在微生物内构建一条由单糖转化到植物生长素的代谢途径。

为实现上述目的，本发明提供了在大肠杆菌和毕赤酵母合成植物生长素苯乙酸、对羟基苯乙醛和吲哚乙酸的方法，主要步骤为：

(1)敲除基因构建菌株gdy

以大肠杆菌mg1655菌株出发，依次敲除该菌株中的trpr基因和七个醛还原酶基因(dkgb、yeae、yqhc、yqhd、dkga、yahk和yjgb),敲除上述8个基因的菌株命名为gdy。

(2)构建产苯乙酸工程菌gdy1

将来自酿酒酵母菌的kdc和aro8基因及来自大肠杆菌的aldh基因，重组到载体pet28a(+)上，得到载体pdy001。将来自大肠杆菌的arog^fbr基因和phea^fbr基因，重组到载体pbbr1mcs1上，得到载体pdy002。将载体pdy001和pdy002转入到大肠杆菌gdy中，实现上述5个基因在大肠杆菌中的高量表达，得到大肠杆菌工程菌株gdy1。表达解除反馈抑制的arog^fbr基因和phea^fbr基因用于加强大肠杆菌胞内苯丙氨酸的合成。表达aro8用于催化苯丙氨酸转化苯丙酮酸，表达kdc用于催化苯丙酮酸脱羧为苯乙醛，表达aldh用于随后催化苯乙醛氧化形成苯乙酸。上述构建的gdy1工程菌可有效的将单糖转化为苯乙酸。

(3)构建产对羟基苯乙酸工程菌gdy2

将来自大肠杆菌的arog^fbr基因和tyra^fbr基因，重组到载体pbbr1mcs1上，得到载体pdy003。将pdy003和前面构建好的载体pdy001同时转入到大肠杆菌gdy中，实现arog^fbr、tyra^fbr、kdc、aro8和aldh5个基因在大肠杆菌中的高量表达，得到大肠杆菌工程菌株gdy2。表达解除反馈抑制的arog^fbr基因和tyra^fbr基因用于加强大肠杆菌胞内酪氨酸的合成。表达aro8用于催化酪氨酸转化对羟基苯丙酮酸，表达kdc用于催化对羟基苯丙酮酸脱羧为对羟基苯乙醛，表达aldh用于随后催化对羟基苯乙醛氧化形成对羟基苯乙酸。上述构建的gdy2工程菌可有效的将单糖转化为对羟基苯乙酸。

(4)构建产吲哚乙酸工程菌gdy3

将来自大肠杆菌的arog^fbr、trpe^fbr和sera^fbr基因，重组到载体pbbr1mcs1上，得到载体pdy004。trpabcd基因(包含trpa、trpb、trpc和trpd四个基因)重组到载体pdy001上，得到载体pdy005。将载体pdy004和pdy005同时转入到大肠杆菌gdy中，实现arog^fbr、trpe^fbr、sera^fbr、trpabcd、kdc、aro8和aldh7个基因在大肠杆菌中的高量表达，得到大肠杆菌工程菌株gdy3。表达解除反馈抑制的arog^fbr、trpe^fbr和sera^fbr基因及trpabcd基因用于加强大肠杆菌胞内色氨酸的合成。表达aro8用于催化色氨酸转化为吲哚丙酮酸，表达kdc用于催化吲哚丙酮酸脱羧为吲哚乙醛，表达aldh用于随后催化吲哚乙醛氧化形成吲哚乙酸。上述构建的gdy3工程菌可有效的将单糖转化为吲哚乙酸。

(5)构建产植物生长素工程菌gdy4

将来自大肠杆菌的aldh基因，重组到载体pgapza,得到载体pdy006。将来自酿酒酵母菌的kdc和aro8基因，分别重组到载体pgapza,得到载体pdy14和pdy15。将pdy14和pdy15分别以bamhi和bglii双酶切，得到kdc基因表达框(包含gap启动子，kdc基因和aox1tt)和aro8基因表达框(包含gap启动子，aro8基因和aox1tt)。将kdc基因表达框和aro8基因表达框分别重组到pao815载体，得到载体pdy007和pdy008。将载体pdy006、pdy007和pdy008分别线性化后，依次导入毕赤酵母gs115中，实现aro8、aldh和kdc基因的组成型表达，该工程菌命名为gdy4。表达aro8用于催化苯丙氨酸、酪氨酸和色氨酸转化为苯丙酮酸、对羟基苯丙酮和吲哚丙酮酸。表达kdc用于催化苯丙酮酸、对羟基苯丙酮酸和吲哚丙酮酸分别转化为苯乙醛、对羟基苯乙醛和吲哚乙醛。表达aldh用于催化苯乙醛、对羟基苯乙醛和吲哚乙醛分别转化为苯乙酸、对羟基苯乙酸和吲哚乙酸。

本发明的优点是在微生物中引入外源基因构建一条合成植物生长素的代谢途径，最终实现以单糖为原料合成植物生长素的目的，不需要通过过多的化学合成反应，减少化学合成过程中有毒物质的释放，也减少了合成成本。同时，由于微生物生长速度快，遗传操作技术成熟，且发酵过程不会对环境造成污染破坏。本发明的毕赤酵母菌株可作为农业微生物，直接利用环境土壤中的营养物质合成植物生长素，从而促进植物的生长。

附图说明

图1.大肠杆菌工程菌gdy1发酵产物gc-ms检测结果。1号峰代表内标苯甲酸,2号峰代表产物苯乙酸。

图2.大肠杆菌工程菌gdy2发酵产物gc-ms检测结果。1号峰代表内标苯甲酸,2号峰代表副产物苯乙酸，3号峰代表产物对羟基苯乙酸。

图3.大肠杆菌工程菌gdy3发酵产物gc-ms检测结果。1号峰代表内标苯甲酸,2号峰代表副产物苯乙酸，3号峰代表副产物对羟基苯乙酸，4号峰代表产物吲哚乙酸。

图4.毕赤酵母工程菌gdy4发酵产物gc-ms检测结果。1号峰代表内标苯甲酸,2号峰代表产物吲哚乙酸,3号峰代表对羟基苯乙酸，4号峰代表吲哚乙酸。

具体实施方式

以下实施例用于进一步说明本发明，但不应理解为对本发明的限制。

实施例1构建产苯乙酸大肠杆菌工程菌

1.基因敲除菌株gdy的构建

以pkd4质粒为模板，pcr扩增打靶片段,该片段包含(kan^r序列和与待敲除目的基因两端互补的同源臂)；将扩增的打靶片段电转到带有pkd46质粒的大肠杆菌中。利用pkd46质粒上带有的red同源重组系统，使待敲除目的基因与打靶片段进行重组，目的基因被抗性基因取代。随后将温敏质粒pcp20导入菌株，利用该质粒上的flp翻转酶去除目的菌株染色体上的抗性基因。利用上述方法依次敲除大肠杆菌mg1655菌株中的trpr、dkgb、yeae、yqhc、yqhd、dkga、yahk和yjgb基因，获得大肠杆菌dgy。所述trpr基因具有seqidno：25所示的核苷酸序列编码；所述trpr基因具有seqidno：26所示的氨基酸序列；所述dkgb基因具有seqidno：27所示的核苷酸序列编码；所述aldh基因具有seqidno：28所示的氨基酸序列；所述yeae基因具有seqidno：29所示的核苷酸序列编码；所述yeae具有seqidno：30所示的氨基酸序列；所述yqhc基因具有seqidno：31所示的核苷酸序列编码；所述yqhc基因具有seqidno：32所示的氨基酸序列；所述yqhd基因具有seqidno：33所示的核苷酸序列编码；所述yqhd基因具有seqidno：34所示的氨基酸序列；所述dkga基因具有seqidno：35所示的核苷酸序列编码；所述dkga基因具有seqidno：36所示的氨基酸序列；所述yahk基因具有seqidno：37所示的核苷酸序列编码；所述yahk基因具有seqidno：38所示的氨基酸序列；所述yjgb基因具有seqidno：39所示的核苷酸序列编码；所述yjgb基因具有seqidno：40所示的氨基酸序列；敲除基因用的引物为：trpr-f:atggcccaacaatcaccctattcagcagcgatggcagaacaggtgtaggctggagctgcttc

trpr-r:tcaatcgcttttcagcaacacctcttccagccactggcgcacatatgaatatcctcctta

dkgb-f:atggctatccctgcatttggtttaggtactttccgtctgagtgtaggctggagctgcttc

dkgb-r:ttaatcccattcaggagccagaccttccgggctaaccaggcggcatatgaatatcctcctta

yeae-f:atgcaacaaaaaatgattcaatttagtggcgatgtctcactggtgtaggctggagctgcttc

yeae-r:tcacaccatatccagcgcagtttttccttttggtgccggatatcatatgaatatcctcctta

yqhc-f:atgctacaaaattgcgcacaatcaaattgccgcattattcctaagtgtaggctggagctgcttc

yqhc-r:ttaattcccctgcatcgcccgcattcttgccgcatcttcccccatatgaatatcctcctta

yqhd-f:atgaacaactttaatctgcacaccccaacccgcattctgttgtgtaggctggagctgcttc

yqhd-r:ttagcgggcggcttcgtatatacggcggctgacatccaacgcatatgaatatcctcctta

dkga-f：atggctaatccaaccgttattaagctacaggatggcaatgtcgtgtaggctggagctgcttc

dkga-r：ttagccgccgaactggtcaggatcgggaccgagacgcttgccatatgaatatcctcctta

yahk-f:atgaagatcaaagctgttggtgcatattccgctaaacaaccgtgtaggctggagctgcttc

yahk-r:tcagtctgttagtgtgcgattatcgataacaaaacgatattcatatgaatatcctcctta

yjgb-f:atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgtgtaggctggagctgcttc

yjgb-r:tcaaaaatcggctttcaacaccacgcggtaacgcgccttaccgtcatatgaatatcctcctta

2.载体pdy001的构建

利用引物kdc-xbai(atctctagatttaagaaggagatataatggcacctgttacaattgaaaagttc)和kdc-nhei-bamhi(tctggatccgctagcctattttttatttcttttaagtgccgctg)pcr扩增来自酿酒酵母菌yph499的kdc基因，随后将扩增的片段以xbai和bamhi插入到pet28a(+)中得到载体pdy01。利用引物aldh-xbai(atctctagatttaagaaggagatataatgaattttcatcatctggcttactg)和aldh-nhei-bamhi(tcaggatccgctagctcaggcctccaggcttatcc)pcr扩增来自大肠杆菌的aldh基因，利用xbai和bamhi插入到pet28a(+)中得到载体pdy02。利用引物aro8-xbai(aactctagatttaagaaggagatataatgatgactttacctgaatcaaaagacttttc)和aro8-nhei-bamhi(acaggatccgctagcctatttggaaataccaaattcttcgtataa)pcr扩增来自酿酒酵母菌yph499的kdc基因，随后将扩增的片段以xbai和bamhi插入到pet28a(+)中得到载体pdy03。利用xbai和xhoi双酶切pdy02得到aldh表达框，将其插入以nhei和xhoi双酶切的载体pdy01上，得到载体pdy04。利用xbai和xhoi双酶切pdy03得到aro8表达框，将其插入以nhei和xhoi双酶切的载体pdy04上，得到载体pdy001。所述kdc基因具有seqidno：19所示的核苷酸序列编码；所述kdc具有seqidno：20所示的氨基酸序列；所述aldh基因具有seqidno：21所示的核苷酸序列编码；所述aldh基因具有seqidno：22所示的氨基酸序列；所述aro8基因具有seqidno：23所示的核苷酸序列编码；所述aro8基因具有seqidno：24所示的氨基酸序列；

3.载体pdy002的构建

解除反馈抑制的arog^fbr和phea^fbr基因委托金唯智生物科技有限公司合成，以合成的基因为模板利用引物arog^fbr-xbai(gtatctagaaagaggagatataatgaattatcagaacgacgatttacgc)和arog^fbr-spei-bamhi(tatggatccactagtttacccgcgacgcgctttta)pcr扩增arog^fbr基因，随后将扩增的片段以xbai和bamhi插入到pet28a(+)中得到载体pdy05。以合成的基因为模板，利用引物phea^fbrr-xbai(gtatctagaaagaggagatataatgacatcggaaaacccgttactg)和phea^fbr-spei-sali(atatgtcgacactagttcacaacgtggttttcgccgga)pcr扩增来phea^fbr基因，随后将扩增的片段以xbai和sali插入到pet28a(+)中得到载体pdy06。利用xbai和xhoi双酶切pdy06得到phea^fbr表达框，将其插入以spei和xhoi双酶切的载体pdy05上，得到载体pdy07。利用xbai和xhoi双酶切pdy07得到arog^fbr和phea^fb表达框，将其插入以xbai和xhoi双酶切的载体pbbr1mcs1上，得到载体pdy002。所述arog^fbr基因具有seqidno：1所示的核苷酸序列；所述arog^fbr基因具有与seqidno：2所示的氨基酸序列；所述phea^fbr基因具有seqidno：3所示的核苷酸序列编码；所述phea^fbr基因具有seqidno：4所示的氨基酸序列；4.将重组载体pdy001和pdy002同时导入大肠杆菌gdy中，得到gdy1工程菌。该工程菌可实现arog^fbr、phea^fbr、kdc、aldh和aro8基因的高表达，可有效的将单糖转化为苯乙酸。

实施例2构建产对羟基苯乙酸大肠杆菌工程菌

1.载体pdy003的构建

解除反馈抑制的tyra^fbr基因委托金唯智生物科技有限公司合成，以该合成的基因为模板利用引物tyra^fbrr-xbai(gtatctagaaagaggagatataatggttgctgaattgaccgcat)和tyra^fbr-spei-sali(atatgtcgacactagtttactggcgattgtcattcgcc)pcr扩增来tyra^fbr基因，随后将扩增的片段以xbai和sali插入到pet28a(+)中得到载体pdy08。利用xbai和xhoi双酶切pdy08得到tyra^fbr表达框，将其插入以spei和xhoi双酶切的载体pdy05上，得到载体pdy09。利用xbai和xhoi双酶切pdy09得到arog^fbr和tyra^fbr表达框，将其插入以xbai和xhoi双酶切的载体pbbr1mcs1上，得到载体pdy003。所述tyra^fbr基因具有seqidno：5所示的核苷酸序列编码；所述tyra^fbr基因具有seqidno：6所示的氨基酸序列；

2.将重组载体pdy001和pdy003同时导入大肠杆菌gdy中，得到gdy2工程菌。该工程菌可实现arog^fbr、tyra^fbr、kdc、aldh和aro8基因的高表达，可有效的将单糖转化为对羟基苯乙酸。

实施例3构建产吲哚乙酸大肠杆菌工程菌

1.载体pdy004的构建

解除反馈抑制的trpe^fbr和sera^fbr基因委托金唯智生物科技有限公司合成。以合成的基因为模板，利用引物trpe^fbr-xbai(gtatctagaaagaggagatataatgcaaacacaaaaaccgactctc)和trpe^fbr-spei-bamhi(tatggatccactagttcagaaagtctcctgtgcatgatg)pcr扩增trpe^fbr基因，随后将扩增的片段以xbai和bamhi插入到pet28a(+)中得到载体pdy10。以合成的基因为模板，利用引物sera^fbr-xbai(gtatctagaaagaggagatataatgacatcggaaaacccgttactg)和sera^fbr-spei-sali(atatgtcgacactagttcacaacgtggttttcgccgga)pcr扩增来sera^fbr基因，随后将扩增的片段以xbai和sali插入到pet28a(+)中得到载体pdy11。利用xbai和xhoi双酶切pdy10得到trpe^fbr表达框，将其插入以spei和xhoi双酶切的载体pdy01上，得到载体pdy12。利用xbai和xhoi双酶切pdy10得到trpe^fbr表达框，将其插入以spei和xhoi双酶切的载体pdy12上，得到载体pdy13。利用xbai和xhoi双酶切pdy13得到sera^fbr和trpe^fbr表达框，将其插入以xbai和xhoi双酶切的载体pbbr1mcs1上，得到载体pdy004。所述trpe^fbr基因具有seqidno：7所示的核苷酸序列编码；所述trpe^fbr基因具有seqidno：8所示的氨基酸序列；sera^fbr所述sera^fbr基因具有seqidno：9所示的核苷酸序列编码；所述sera^fbr基因具有seqidno：10所示的氨基酸序列；

2.载体pdy005的构建

以大肠杆菌mg1655基因组为模板，利用引物trpabcd-xbai(ccatctagatttaagaggagatataatggctgacattctgctgctcgata)和trpabcd-xhoi(gattctcgagttaactgcgcgtcgccgct)pcr扩增trpabcd基因(包含trpa、trpb、trpc和trpd四个基因)，随后将扩增的片段以xbai和xhoi插入到pdy001中,得到载体pdy005。所述trpa具有seqidno：11所示的核苷酸序列编码；trpa基因具有seqidno：12所示的氨基酸序列；所述trpb具有seqidno：13所示的核苷酸序列编码；所述trpb基因具有seqidno：14所示的氨基酸序列；所述trpc具有seqidno：15所示的核苷酸序列编码；所述trpc基因具有seqidno：16所示的氨基酸序列；所述trpd具有seqidno：17所示的核苷酸序列编码所述；所述trpd基因具有seqidno：18所示的氨基酸序列；

3.将重组载体pdy004和pdy005同时导入大肠杆菌gdy中，得到gdy3工程菌。该工程菌可实现arog^fbr、trpe^fbr、sera^fbr、trpabcd、kdc、aldh和aro8基因的高表达，可有效的将单糖转化为吲哚乙酸。

实施例4构建产植物生长素毕赤酵母工程菌gdy4

以大肠杆菌mg1655基因组为模板，利用引物aldh-bstbi(agcattcgaaatgaattttcatcatctggcttactggc)和aldh-xhoi(gaacctcgagtcaggcctccaggcttatccagat)pcr扩增aldhr基因，随后将扩增的片段以bstbi和xhoii插入到pgapza中得到载体pdy006。以酿酒酵母yph499基因组为模板，利用引物kdc-bstbi(agcattcgaaatggcacctgttacaattgaaaagttc)/kdc-xhoi(cttcctcgagctattttttatttcttttaagtgccgctg)、引物aro8-bstbi(agcattcgaaatgactttacctgaatcaaaagacttttc)/aro8-xhoi(cttcctcgagctatttggaaataccaaattcttcgtataa)pcr扩增kdc和aro8基因，随后将扩增的片段以bstbi和xhoi分别插入到pgapza中，得到载体pdy14和pdy15。将pdy14和pdy15分别以bamhi和bglii双酶切，得到kdc基因表达框(包含gap启动子，kdc基因和aox1tt)和aro8基因表达框(包含gap启动子，aro8基因和aox1tt)。将kdc基因表达框重组到经bamhi酶切的pao815载体，得到载体pdy007。将aro8基因表达框重组到经bamhi酶切的ppic3.5k载体，得到载体pdy008。将载体pdy006、pdy007和pdy008分别线性化后，依次导入毕赤酵母gs115中，实现aro8、aldh和kdc基因的组成型表达，该工程菌命名为gdy4。表达aro8用于催化苯丙氨酸、酪氨酸和色氨酸转化为苯丙酮酸、对羟基苯丙酮和吲哚丙酮酸。表达kdc用于催化苯丙酮酸、对羟基苯丙酮酸和吲哚丙酮酸分别转化为苯乙醛、对羟基苯乙醛和吲哚乙醛。表达aldh用于催化苯乙醛、对羟基苯乙醛和吲哚乙醛分别转化为苯乙酸、对羟基苯乙酸和吲哚乙酸。所述kdc基因具有seqidno：19所示的核苷酸序列编码；所述kdc具有seqidno：20所示的氨基酸序列；所述aldh基因具有seqidno：21所示的核苷酸序列编码；所述aldh基因具有seqidno：22所示的氨基酸序列；所述aro8基因具有seqidno：23所示的核苷酸序列编码；所述aro8基因具有seqidno：24所示的氨基酸序列；

实施例5大肠杆菌工程菌生产植物生长素的发酵实验

1、将37度lb中过夜培养的大肠杆菌工程菌gdy1、gdy2和gdy3，分别接种到50mlm9发酵培养基中(包含2％葡萄糖)，进行30度摇瓶发酵，待菌体od600为0.8左右，加入0.1mmiptg进行目的基因的诱导表达。

2、发酵培养24小时后收集菌体，超声波破细胞，利用乙酸乙酯萃取产物。

3、利用旋转蒸发仪将溶剂乙酸乙酯旋干，再用一定量的乙酸乙酯溶解产物。

4、gc-ms(气相色谱-质谱联用仪)检测样品。

5、实验结果：大肠杆菌工程菌利用葡萄糖合成植物生长素的gc-ms检测结果如图1、图2和图3所示。图1为大肠杆菌工程菌gdy1发酵产物gc-ms检测结果，表明菌株gdy1可有效利用葡萄糖合成植物生长素苯乙酸，摇床产量为1.67g/l。图2为大肠杆菌工程菌gdy2发酵产物gc-ms检测结果，表明菌株gdy2可有效利用葡萄糖合成植物生长素对羟基苯乙酸，摇床产量为1.07g/l。图3为大肠杆菌工程菌gdy3发酵产物gc-ms检测结果，表明菌株gdy3可有效利用葡萄糖合成植物生长素对吲哚乙酸，摇床产量为1.16g/l。

实施例6毕赤酵母工程菌生产植物生长素的发酵实验

6、将30度ypd中过夜培养的毕赤酵母工程菌gdy4，接种到50mlypd发酵培养基中(包含2％葡萄糖)，进行30度摇瓶发酵。

7、发酵培养28小时后收集菌体，超声波破细胞，利用乙酸乙酯萃取产物。

8、利用旋转蒸发仪将溶剂乙酸乙酯旋干，再用一定量的乙酸乙酯溶解产物。

9、gc-ms(气相色谱-质谱联用仪)检测样品。

10、实验结果：毕赤酵母工程菌利用葡萄糖合成植物生长素的gc-ms检测结果如图4所示。结果表明菌株gdy4可有效利用葡萄糖合成植物生长素苯乙酸，对羟基苯乙酸和吲哚乙酸，产量分别为108mg/l,36mg/l和33mg/l。

以上所述仅是本发明的优选实施方案，应当指出，对于本技术领域的技术人员，在不脱离本分明原理的前提下，还可以做出适当的改进，这些改进也应视为本发明的保护范围。

序列表

<110>赣南师范大学

<120>一种微生物合成植物生长素的方法

<160>55

<170>siposequencelisting1.0

<210>1

<211>1053

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>1

atgaattatcagaacgacgatttacgcatcaaagaaatcaaagagttacttcctcctgtc60

gcattgctggaaaaattccccgctactgaaaatgccgcgaatacggttgcccatgcccga120

aaagcgatccataagatcctgaaaggtaatgatgatcgcctgttggttgtgattggccca180

tgctcaattcatgatcctgtcgcggcaaaagagtatgccactcgcttgctggcgctgcgt240

gaagagctgaaagatgagctggaaatcgtaatgcgcgtctattttgaaaagccgcgtacc300

acggtgggctggaaagggctgattaacgatccgcatatggataatagcttccagatcaac360

gacggtctgcgtatagcccgtaaattgctgcttgatattaacgacagcggtctgccagcg420

gcaggtgagtttctcaatatgatcaccccacaatatctcgctgacctgatgagctggggc480

gcaattggcgcacgtaccaccgaatcgcaggtgcaccgcgaactggcatcagggctttct540

tgtccggtcggcttcaaaaatggcaccgacggtacgattaaagtggctatcgatgccatt600

aatgccgccggtgcgccgcactgcttcctgtccgtaacgaaatgggggcattcggcgatt660

gtgaataccagcggtaacggcgattgccatatcattctgcgcggcggtaaagagcctaac720

tacagcgcgaagcacgttgctgaagtgaaagaagggctgaacaaagcaggcctgccagca780

caggtgatgatcgatttcagccatgctaactcgtccaaacaattcaaaaagcagatggat840

gtttgtgctgacgtttgccagcagattgccggtggcgaaaaggccattattggcgtgatg900

gtggaaagccatctggtggaaggcaatcagagcctcgagagcggggagccgctggcctac960

ggtaagagcatcaccgatgcctgcatcggctgggaagataccgatgctctgttacgtcaa1020

ctggcgaatgcagtaaaagcgcgtcgcgggtaa1053

<210>3

<211>350

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>3

metasntyrglnasnaspaspleuargilelysgluilelysgluleu

151015

leuproprovalalaleuleuglulyspheproalathrgluasnala

202530

alaasnthrvalalahisalaarglysalailehislysileleulys

354045

glyasnaspaspargleuleuvalvalileglyprocysserilehis

505560

aspprovalalaalalysglutyralathrargleuleualaleuarg

65707580

glugluleulysaspgluleugluilevalmetargvaltyrpheglu

859095

lysproargthrthrvalglytrplysglyleuileasnaspprohis

100105110

metaspasnserpheglnileasnaspglyleuargilealaarglys

115120125

leuleuleuaspileasnaspserglyleuproalaalaglygluphe

130135140

leuasnmetilethrproglntyrleualaaspleumetsertrpgly

145150155160

alaileglyalaargthrthrgluserglnvalhisarggluleuala

165170175

serglyleusercysprovalglyphelysasnglythraspglythr

180185190

ilelysvalalaileaspalaileasnalaalaglyalaprohiscys

195200205

pheleuservalthrlystrpglyhisseralailevalasnthrser

210215220

glyasnglyaspcyshisileileleuargglyglylysgluproasn

225230235240

tyrseralalyshisvalalagluvallysgluglyleuasnlysala

245250255

glyleuproalaglnvalmetileasppheserhisalaasnserser

260265270

lysglnphelyslysglnmetaspvalcysalaaspvalcysglngln

275280285

ilealaglyglyglulysalaileileglyvalmetvalgluserhis

290295300

leuvalgluglyasnglnserleugluserglygluproleualatyr

305310315320

glylysserilethraspalacysileglytrpgluaspthraspala

325330335

leuleuargglnleualaasnalavallysalaargarggly

340345350

<210>3

<211>903

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>3

atgacatcggaaaacccgttactggcgctgcgagagaaaatcagcgcgctggatgaaaaa60

ttattagcgttactggcagaacggcgcgaactggccgtcgaggtgggaaaagccaaactg120

ctctcgcatcgcccggtacgtgatattgatcgtgaacgcgatttgctggaaagattaatt180

acgctcggtaaagcgcaccatctggacgcccattacattactcgcctgttccagctcatc240

attgaagattccgtattaactcagcaggctttgctccaacaacatctcaataaaattaat300

ccgcactcagcacgcatcgcttttctcggccccaaaggttcttattcccatcttgcggcg360

cgccagtatgctgcccgtcactttgagcaattcattgaaagtggctgcgccaaatttgcc420

gatatttttaatcaggtggaaaccggccaggccgactatgccgtcgtaccgattgaaaat480

accagctccggtgccataaacgacgtttacgatctgctgcaacataccagcttgtcgatt540

gttggcgagatgacgttaactatcgaccattgtttgttggtctccggcactactgattta600

tccaccatcaatacggtctacagccatccgcagccattccagcaatgcagcaaattcctt660

aatcgttatccgcactggaagattgaatataccgaaagtacgtctgcggcaatggaaaag720

gttgcacaggcaaaatcaccgcatgttgctgcgttgggaagcgaagctggcggcactttg780

tacggtttgcaggtactggagcgtattgaagcaaatcagcgacaaaacttcacccgattt840

gtggtgttggcgcgtaaagccattaacgtgtctgatcaggttccggcgaaaaccacgttg900

tga903

<210>4

<211>300

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>4

metthrsergluasnproleuleualaleuargglulysileserala

151015

leuaspglulysleuleualaleuleualagluargarggluleuala

202530

valgluvalglylysalalysleuleuserhisargprovalargasp

354045

ileasparggluargaspleuleugluargleuilethrleuglylys

505560

alahishisleuaspalahistyrilethrargleupheglnleuile

65707580

ilegluaspservalleuthrglnglnalaleuleuglnglnhisleu

859095

asnlysileasnprohisseralaargilealapheleuglyprolys

100105110

glysertyrserhisleualaalaargglntyralaalaarghisphe

115120125

gluglnpheilegluserglycysalalysphealaaspilepheasn

130135140

glnvalgluthrglyglnalaasptyralavalvalproilegluasn

145150155160

thrserserglyalaileasnaspvaltyraspleuleuglnhisthr

165170175

serleuserilevalglyglumetthrleuthrileasphiscysleu

180185190

leuvalserglythrthraspleuserthrileasnthrvaltyrser

195200205

hisproglnpropheglnglncysserlyspheleuasnargtyrpro

210215220

histrplysileglutyrthrgluserthrseralaalametglulys

225230235240

valalaglnalalysserprohisvalalaalaleuglysergluala

245250255

glyglythrleutyrglyleuglnvalleugluargileglualaasn

260265270

glnargglnasnphethrargphevalvalleualaarglysalaile

275280285

asnvalseraspglnvalproalalysthrthrleu

290295300

<210>5

<211>1122

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>5

atggttgctgaattgaccgcattacgcgatcaaattgatgaagtcgataaagcgctgctg60

aatttattagcgaagcgtctggaactggttgctgaagtgggcgaggtgaaaagccgcttt120

ggactgcctatttatgttccggagcgcgaggcatctattttggcctcgcggcgcgcagag180

gcggaagctctgggtgtaccgccagatctgattgaggatgttttgcgtcgggtgatgcgt240

gaatcttactccagtgaaaacgacaaaggatttaaaacactttgtccgtcactgcgtccg300

gtggttatcgtcggcggtggcggtcagatgggacgcctgttcgagaagatgctgacacta360

tcgggttatcaggtgcggattctggagcaacatgactgggatcgagcggctgatattgtt420

gccgatgccggaatggtgattgttagtgtgccaatccacgttactgagcaagttatcggc480

aaattaccgcctttaccgaaagattgtattctggttgatctggcatcagtgaaaaatgga540

ccattacaggccatgctggcggcgcacgatggcccggtactggggttacacccgatgttc600

ggcccggacagcggtagcctggcaaagcaagttgtggtctggtgtgatggacgtaagccg660

gaagcataccaatggtttctggagcaaattcaggtctggggcgctcggctgcatcgtatt720

agcgctgtcgagcacgatcagaatatggcgtttattcaggctctgcgccactttgctact780

tttgcttatgggctgcatctggcagaagaaaatgttcagcttgagcaacttctggcgctc840

tcttcgccgatttaccgccttgagctggcgatggtcgggcgactgtttgctcaggatccg900

cagctttatgccgacattattatgtcgtcagagcgtaatctggcgttaatcaaacgttac960

tataagcgtttcggcgaggcgattgagttgctggagcagggcgataagcaggcgtttatt1020

gacagtttccgcaaggtggagcactggttcggcgattacgttcagcgttttcagagtgaa1080

agccgcgtgttattgcgtcaggcgaatgacaatcgccagtaa1122

<210>6

<211>373

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>6

metvalalagluleuthralaleuargaspglnileaspgluvalasp

151015

lysalaleuleuasnleuleualalysargleugluleuvalalaglu

202530

valglygluvallysserargpheglyleuproiletyrvalproglu

354045

argglualaserileleualaserargargalaglualaglualaleu

505560

glyvalproproaspleuilegluaspvalleuargargvalmetarg

65707580

glusertyrsersergluasnasplysglyphelysthrleucyspro

859095

serleuargprovalvalilevalglyglyglyglyglnmetglyarg

100105110

leupheglulysmetleuthrleuserglytyrglnvalargileleu

115120125

gluglnhisasptrpaspargalaalaaspilevalalaaspalagly

130135140

metvalilevalservalproilehisvalthrgluglnvalilegly

145150155160

lysleuproproleuprolysaspcysileleuvalaspleualaser

165170175

vallysasnglyproleuglnalametleualaalahisaspglypro

180185190

valleuglyleuhisprometpheglyproaspserglyserleuala

195200205

lysglnvalvalvaltrpcysaspglyarglysproglualatyrgln

210215220

trppheleugluglnileglnvaltrpglyalaargleuhisargile

225230235240

seralavalgluhisaspglnasnmetalapheileglnalaleuarg

245250255

hisphealathrphealatyrglyleuhisleualaglugluasnval

260265270

glnleugluglnleuleualaleuserserproiletyrargleuglu

275280285

leualametvalglyargleuphealaglnaspproglnleutyrala

290295300

aspileilemetsersergluargasnleualaleuilelysargtyr

305310315320

tyrlysargpheglyglualailegluleuleugluglnglyasplys

325330335

glnalapheileaspserphearglysvalgluhistrppheglyasp

340345350

tyrvalglnargpheglnsergluserargvalleuleuargglnala

355360365

asnaspasnarggln

370

<210>7

<211>1563

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>7

atgcaaacacaaaaaccgactctcgaacagctaacctgcgaaggcgcttatcgcgacaat60

cccaccgcgctttttcaccagttgtgtggggatcgtccggcaacgctgctgctggaattt120

gcagatatcgacagcaaagatgatttaaaaagcctgctgctggtagacagtgcgctgcgc180

attacagctttaggtgacactgtcacaatccaggcactttccggcaacggcgaagccctg240

ctggcactactggataacgccctgcctgcgggtgtggaaagtgaacaatcaccaaactgc300

cgtgtgctgcgcttcccccctgtcagtccactgctggatgaagacgcccgcttatgctcc360

ctttcggtttttgacgctttccgtttattgcagaatctgttgaatgtaccgaaggaagaa420

cgagaagccatgttcttcggcggcctgttctcttatgaccttgtggcgggatttgaagat480

ttaccgcaactgtcagcggaaaataactgccctgatttctgtttttatctcgctgaaacg540

ctgatggtgattgaccatcagaaaaaaagcacccgtattcaggccagcctgtttgctccg600

aatgaagaagaaaaacaacgtctcactgctcgcctgaacgaactacgtcagcaactgacc660

gaagccgcgccgccgctgccagtggtttccgtgccgcatatgcgttgtgaatgtaatcag720

agcgatgaagagttcggtggcgtagtgcgtttgttgcaaaaagcgattcgcgctggagaa780

attttccaggtggtgccatctcgccgtttctctctgccctgcccgtcaccgctggcggcc840

tattacgtgctgaaaaagagtaatcccagcccgtacatgttttttatgcaggataatgat900

ttcaccctatttggcgcgtcgccggaaagctcgctcaagtatgatgccaccagccgccag960

attgagatctacccgattgccggaacacgcccacgcggtcgtcgcgccgatggttcactg1020

gacagagatctcgacagccgtattgaactggaaatgcgtaccgatcataaagagctgtct1080

gaacatctgatgctggttgatctcgcccgtaatgatctggcacgcatttgcacccccggc1140

agccgctacgtcgccgatctcaccaaagttgaccgttattcctatgtgatgcacctcgtc1200

tctcgcgtagtcggcgaactgcgtcacgatcttgacgccctgcacgcttatcgcgcctgt1260

atgaatatggggacgttaagcggtgcgccgaaagtacgcgctatgcagttaattgccgag1320

gcggaaggtcgtcgccgcggcagctacggcggcgcggtaggttatttcaccgcgcatggc1380

gatctcgacacctgcattgtgatccgctcggcgctggtggaaaacggtatcgccaccgtg1440

caagcgggtgctggtgtagtccttgattctgttccgcagtcggaagccgacgaaacccgt1500

aacaaagcccgcgctgtactgcgcgctattgccaccgcgcatcatgcacaggagactttc1560

tga1563

<210>8

<211>520

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>8

metglnthrglnlysprothrleugluglnleuthrcysgluglyala

151015

tyrargaspasnprothralaleuphehisglnleucysglyasparg

202530

proalathrleuleuleugluphealaaspileaspserlysaspasp

354045

leulysserleuleuleuvalaspseralaleuargilethralaleu

505560

glyaspthrvalthrileglnalaleuserglyasnglyglualaleu

65707580

leualaleuleuaspasnalaleuproalaglyvalgluserglugln

859095

serproasncysargvalleuargpheproprovalserproleuleu

100105110

aspgluaspalaargleucysserleuservalpheaspalaphearg

115120125

leuleuglnasnleuleuasnvalprolysglugluargglualamet

130135140

phepheglyglyleuphesertyraspleuvalalaglyphegluasp

145150155160

leuproglnleuseralagluasnasncysproaspphecysphetyr

165170175

leualagluthrleumetvalileasphisglnlyslysserthrarg

180185190

ileglnalaserleuphealaproasnglugluglulysglnargleu

195200205

thralaargleuasngluleuargglnglnleuthrglualaalapro

210215220

proleuprovalvalservalprohismetargcysglucysasngln

225230235240

seraspgluglupheglyglyvalvalargleuleuglnlysalaile

245250255

argalaglygluilepheglnvalvalproserargargpheserleu

260265270

procysproserproleualaalatyrtyrvalleulyslysserasn

275280285

proserprotyrmetphephemetglnaspasnaspphethrleuphe

290295300

glyalaserprogluserserleulystyraspalathrserarggln

305310315320

ilegluiletyrproilealaglythrargproargglyargargala

325330335

aspglyserleuaspargaspleuaspserargilegluleuglumet

340345350

argthrasphislysgluleusergluhisleumetleuvalaspleu

355360365

alaargasnaspleualaargilecysthrproglyserargtyrval

370375380

alaaspleuthrlysvalaspargtyrsertyrvalmethisleuval

385390395400

serargvalvalglygluleuarghisaspleuaspalaleuhisala

405410415

tyrargalacysmetasnmetglythrleuserglyalaprolysval

420425430

argalametglnleuilealaglualagluglyargargargglyser

435440445

tyrglyglyalavalglytyrphethralahisglyaspleuaspthr

450455460

cysilevalileargseralaleuvalgluasnglyilealathrval

465470475480

glnalaglyalaglyvalvalleuaspservalproglnsergluala

485490495

aspgluthrargasnlysalaargalavalleuargalailealathr

500505510

alahishisalaglngluthrphe

515520

<210>9

<211>1233

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>9

atggcaaaggtatcgctggagaaagacaagattaagtttctgctggtagaaggcgtgcac60

caaaaggcgctggaaagccttcgtgcagctggttacaccaacatcgaatttcacaaaggc120

gcgctggatgatgaacaattaaaagaatccatccgcgatgcccacttcatcggcctgcga180

tcccgtacccatctgactgaagacgtgatcaacgccgcagaaaaactggtcgctattggc240

tgtttctgtatcggaacaaaccaggttgatctggatgcggcggcaaagcgcgggatcccg300

gtatttaacgcaccgttctcaaatacgcgctctgttgcggagctggtgattggcgaactg360

ctgctgctattgcgcggcgtgccggaagccaatgctaaagcgcaccgtggcgtgtggaac420

aaactggcggcgggttcttttgaagcgcgcggcaaaaagctgggtatcatcggctacggt480

catattggtacgcaattgggcattctggctgaatcgctgggaatgtatgtttacttttat540

gatattgaaaataaactgccgctgggcaacgccactcaggtacagcatctttctgacctg600

ctgaatatgagcgatgtggtgagtctgcatgtaccagagaatccgtccaccaaaaatatg660

atgggcgcgaaagaaatttcactaatgaagcccggctcgctgctgattaatgcttcgcgc720

ggtactgtggtggatattccggcgctgtgtgatgcgctggcgagcaaacatctggcgggg780

gcggcaatcgacgtattcccgacggaaccggcgaccaatagcgatccatttacctctccg840

ctgtgtgaattcgacaacgtccttctgacgccacacattggcggttcgactcaggaagcg900

caggagaatatcggcctggaagttgcgggtaaattgatcaagtattctgacaatggctca960

acgctctctgcggtgaacttcccggaagtctcgctgccactgcacggtgggcgtcgtctg1020

atgcacatcgcagaaaaccgtccgggcgtgctaactgcgctgaacaaaatcttcgccgag1080

cagggcgtcgcaatcgccgcgcaatatctgcaaacttccgcccagatgggttatgtggtt1140

attgatattgaagccgacgaagacgttgccgaaaaagcgctgcaggcaatgaaagctatt1200

ccgggtaccattcgcgcccgtctgctgtactaa1233

<210>10

<211>410

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>10

metalalysvalserleuglulysasplysilelyspheleuleuval

151015

gluglyvalhisglnlysalaleugluserleuargalaalaglytyr

202530

thrasnilegluphehislysglyalaleuaspaspgluglnleulys

354045

gluserileargaspalahispheileglyleuargserargthrhis

505560

leuthrgluaspvalileasnalaalaglulysleuvalalailegly

65707580

cysphecysileglythrasnglnvalaspleuaspalaalaalalys

859095

argglyileprovalpheasnalapropheserasnthrargserval

100105110

alagluleuvalileglygluleuleuleuleuleuargglyvalpro

115120125

glualaasnalalysalahisargglyvaltrpasnlysleualaala

130135140

glyserpheglualaargglylyslysleuglyileileglytyrgly

145150155160

hisileglythrglnleuglyileleualagluserleuglymettyr

165170175

valtyrphetyraspilegluasnlysleuproleuglyasnalathr

180185190

glnvalglnhisleuseraspleuleuasnmetseraspvalvalser

195200205

leuhisvalprogluasnproserthrlysasnmetmetglyalalys

210215220

gluileserleumetlysproglyserleuleuileasnalaserarg

225230235240

glythrvalvalaspileproalaleucysaspalaleualaserlys

245250255

hisleualaglyalaalaileaspvalpheprothrgluproalathr

260265270

asnseraspprophethrserproleucysglupheaspasnvalleu

275280285

leuthrprohisileglyglyserthrglnglualaglngluasnile

290295300

glyleugluvalalaglylysleuilelystyrseraspasnglyser

305310315320

thrleuseralavalasnpheprogluvalserleuproleuhisgly

325330335

glyargargleumethisilealagluasnargproglyvalleuthr

340345350

alaleuasnlysilephealagluglnglyvalalailealaalagln

355360365

tyrleuglnthrseralaglnmetglytyrvalvalileaspileglu

370375380

alaaspgluaspvalalaglulysalaleuglnalametlysalaile

385390395400

proglythrileargalaargleuleutyr

405410

<210>11

<211>807

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>11

atggaacgctacgaatctctgtttgcccagttgaaggagcgcaaagaaggcgcattcgtt60

cctttcgtcacgctcggtgatccgggcattgagcagtcattgaaaattatcgatacgcta120

attgaagccggtgctgacgcgctggagttaggtatccccttctccgacccactggcggat180

ggcccgacgattcaaaacgccactctgcgcgcctttgcggcaggtgtgactccggcacaa240

tgttttgaaatgctggcactgattcgccagaaacacccgaccattcccattggcctgttg300

atgtatgccaatctggtgtttaacaaaggcattgatgagttttatgcccagtgcgaaaaa360

gtcggcgtcgattcggtgctggttgccgatgtgccagttgaagagtccgcgcccttccgc420

caggccgcgttgcgtcataatgtcgcacctatcttcatctgcccgccaaatgccgatgac480

gacctgctgcgccagatagcctcttacggtcgtggttacacctatttgctgtcacgagca540

ggcgtgaccggcgcagaaaaccgcgccgcgttacccctcaatcatctggttgcgaagctg600

aaagagtacaacgctgcacctccattgcagggatttggtatttccgccccggatcaggta660

aaagcagcgattgatgcaggagctgcgggcgcgatttctggttcggccattgttaaaatc720

atcgagcaacatattaatgagccagagaaaatgctggcggcactgaaagtttttgtacaa780

ccgatgaaagcggcgacgcgcagttaa807

<210>12

<211>268

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>12

metgluargtyrgluserleuphealaglnleulysgluarglysglu

151015

glyalaphevalprophevalthrleuglyaspproglyileglugln

202530

serleulysileileaspthrleuileglualaglyalaaspalaleu

354045

gluleuglyilepropheseraspproleualaaspglyprothrile

505560

glnasnalathrleuargalaphealaalaglyvalthrproalagln

65707580

cyspheglumetleualaleuileargglnlyshisprothrilepro

859095

ileglyleuleumettyralaasnleuvalpheasnlysglyileasp

100105110

gluphetyralaglncysglulysvalglyvalaspservalleuval

115120125

alaaspvalprovalglugluseralapropheargglnalaalaleu

130135140

arghisasnvalalaproilepheilecysproproasnalaaspasp

145150155160

aspleuleuargglnilealasertyrglyargglytyrthrtyrleu

165170175

leuserargalaglyvalthrglyalagluasnargalaalaleupro

180185190

leuasnhisleuvalalalysleulysglutyrasnalaalapropro

195200205

leuglnglypheglyileseralaproaspglnvallysalaalaile

210215220

aspalaglyalaalaglyalaileserglyseralailevallysile

225230235240

ilegluglnhisileasngluproglulysmetleualaalaleulys

245250255

valphevalglnprometlysalaalathrargser

260265

<210>13

<211>1194

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>13

atgacaacattacttaacccctattttggtgagtttggcggcatgtacgtgccacaaatc60

ctgatgcctgctctgcgccagctggaagaagcttttgtcagtgcgcaaaaagatcctgaa120

tttcaggctcagttcaacgacctgctgaaaaactatgccgggcgtccaaccgcgctgacc180

aaatgccagaacattacagccgggacgaacaccacgctgtatctcaagcgtgaagatttg240

ctgcacggcggcgcgcataaaactaaccaggtgctggggcaggcgttgctggcgaagcgg300

atgggtaaaaccgaaatcatcgccgaaaccggtgccggtcagcatggcgtggcgtcggcc360

cttgccagcgccctgctcggcctgaaatgccgtatttatatgggtgccaaagacgttgaa420

cgccagtcgcctaacgtttttcgtatgcgcttaatgggtgcggaagtgatcccggtgcat480

agcggttccgcgacgctgaaagatgcctgtaacgaggcgctgcgcgactggtccggtagt540

tacgaaaccgcgcactatatgctgggcaccgcagctggcccgcatccttatccgaccatt600

gtgcgtgagtttcagcggatgattggcgaagaaaccaaagcgcagattctggaaagagaa660

ggtcgcctgccggatgccgttatcgcctgtgttggcggcggttcgaatgccatcggcatg720

tttgctgatttcatcaatgaaaccaacgtcggcctgattggtgtggagccaggtggtcac780

ggtatcgaaactggcgagcacggcgcaccgctaaaacatggtcgcgtgggtatctatttc840

ggtatgaaagcgccgatgatgcaaaccgaagacgggcagattgaagaatcttactccatc900

tccgccggactggatttcccgtctgtcggcccacaacacgcgtatcttaacagcactgga960

cgcgctgattacgtgtctattaccgatgatgaagcccttgaagccttcaaaacgctgtgc1020

ctgcacgaagggatcatcccggcgctggaatcctcccacgccctggcccatgcgttgaaa1080

atgatgcgcgaaaacccggataaagagcagctactggtggttaacctttccggtcgcggc1140

gataaagacatcttcaccgttcacgatattttgaaagcacgaggggaaatctga1194

<210>14

<211>397

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>14

metthrthrleuleuasnprotyrpheglyglupheglyglymettyr

151015

valproglnileleumetproalaleuargglnleugluglualaphe

202530

valseralaglnlysaspproglupheglnalaglnpheasnaspleu

354045

leulysasntyralaglyargprothralaleuthrlyscysglnasn

505560

ilethralaglythrasnthrthrleutyrleulysarggluaspleu

65707580

leuhisglyglyalahislysthrasnglnvalleuglyglnalaleu

859095

leualalysargmetglylysthrgluileilealagluthrglyala

100105110

glyglnhisglyvalalaseralaleualaseralaleuleuglyleu

115120125

lyscysargiletyrmetglyalalysaspvalgluargglnserpro

130135140

asnvalpheargmetargleumetglyalagluvalileprovalhis

145150155160

serglyseralathrleulysaspalacysasnglualaleuargasp

165170175

trpserglysertyrgluthralahistyrmetleuglythralaala

180185190

glyprohisprotyrprothrilevalargglupheglnargmetile

195200205

glyglugluthrlysalaglnileleugluarggluglyargleupro

210215220

aspalavalilealacysvalglyglyglyserasnalaileglymet

225230235240

phealaasppheileasngluthrasnvalglyleuileglyvalglu

245250255

proglyglyhisglyilegluthrglygluhisglyalaproleulys

260265270

hisglyargvalglyiletyrpheglymetlysalaprometmetgln

275280285

thrgluaspglyglnilegluglusertyrserileseralaglyleu

290295300

asppheproservalglyproglnhisalatyrleuasnserthrgly

305310315320

argalaasptyrvalserilethraspaspglualaleuglualaphe

325330335

lysthrleucysleuhisgluglyileileproalaleugluserser

340345350

hisalaleualahisalaleulysmetmetarggluasnproasplys

355360365

gluglnleuleuvalvalasnleuserglyargglyasplysaspile

370375380

phethrvalhisaspileleulysalaargglygluile

385390395

<210>15

<211>1362

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>15

atgatgcaaaccgttttagcgaaaatcgtcgcagacaaggcgatttgggtagaagcccgc60

aaacagcagcaaccgctggccagttttcagaatgaggttcagccgagcacgcgacatttt120

tatgatgcgctacagggtgcgcgcacggcgtttattctggagtgcaagaaagcgtcgccg180

tcaaaaggcgtgatccgtgatgatttcgatccagcacgcattgccgccatttataaacat240

tacgcttcggcaatttcggtgctgactgatgagaaatattttcaggggagctttaatttc300

ctccccatcgtcagccaaatcgccccgcagccgattttatgtaaagacttcattatcgac360

ccttaccagatctatctggcgcgctattaccaggccgatgcctgcttattaatgctttca420

gtactggatgacgaccaatatcgccagcttgccgccgtcgctcacagtctggagatgggg480

gtgctgaccgaagtcagtaatgaagaggaacaggagcgcgccattgcattgggagcaaag540

gtcgttggcatcaacaaccgcgatctgcgtgatttgtcgattgatctcaaccgtacccgc600

gagcttgcgccgaaactggggcacaacgtgacggtaatcagcgaatccggcatcaatact660

tacgctcaggtgcgcgagttaagccacttcgctaacggttttctgattggttcggcgttg720

atggcccatgacgatttgcacgccgccgtgcgccgggtgttgctgggtgagaataaagta780

tgtggcctgacgcgtgggcaagatgctaaagcagcttatgacgcgggcgcgatttacggt840

gggttgatttttgttgcgacatcaccgcgttgcgtcaacgttgaacaggcgcaggaagtg900

atggctgcggcaccgttgcagtatgttggcgtgttccgcaatcacgatattgccgatgtg960

gtggacaaagctaaggtgttatcgctggcggcagtgcaactgcatggtaatgaagaacag1020

ctgtatatcgatacgctgcgtgaagctctgccagcacatgttgccatctggaaagcatta1080

agcgtcggtgaaaccctgcccgcccgcgagtttcagcacgttgataaatatgttttagac1140

aacggccagggtggaagcgggcaacgttttgactggtcactattaaatggtcaatcgctt1200

ggcaacgttctgctggcggggggcttaggcgcagataactgcgtggaagcggcacaaacc1260

ggctgcgccggacttgattttaattctgctgtagagtcgcaaccgggcatcaaagacgca1320

cgtcttttggcctcggttttccagacgctgcgcgcatattaa1362

<210>16

<211>453

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>16

metmetglnthrvalleualalysilevalalaasplysalailetrp

151015

valglualaarglysglnglnglnproleualaserpheglnasnglu

202530

valglnproserthrarghisphetyraspalaleuglnglyalaarg

354045

thralapheileleuglucyslyslysalaserproserlysglyval

505560

ileargaspasppheaspproalaargilealaalailetyrlyshis

65707580

tyralaseralaileservalleuthraspglulystyrpheglngly

859095

serpheasnpheleuproilevalserglnilealaproglnproile

100105110

leucyslysasppheileileaspprotyrglniletyrleualaarg

115120125

tyrtyrglnalaaspalacysleuleumetleuservalleuaspasp

130135140

aspglntyrargglnleualaalavalalahisserleuglumetgly

145150155160

valleuthrgluvalserasngluglugluglngluargalaileala

165170175

leuglyalalysvalvalglyileasnasnargaspleuargaspleu

180185190

serileaspleuasnargthrarggluleualaprolysleuglyhis

195200205

asnvalthrvalilesergluserglyileasnthrtyralaglnval

210215220

arggluleuserhisphealaasnglypheleuileglyseralaleu

225230235240

metalahisaspaspleuhisalaalavalargargvalleuleugly

245250255

gluasnlysvalcysglyleuthrargglyglnaspalalysalaala

260265270

tyraspalaglyalailetyrglyglyleuilephevalalathrser

275280285

proargcysvalasnvalgluglnalaglngluvalmetalaalaala

290295300

proleuglntyrvalglyvalpheargasnhisaspilealaaspval

305310315320

valasplysalalysvalleuserleualaalavalglnleuhisgly

325330335

asnglugluglnleutyrileaspthrleuargglualaleuproala

340345350

hisvalalailetrplysalaleuservalglygluthrleuproala

355360365

argglupheglnhisvalasplystyrvalleuaspasnglyglngly

370375380

glyserglyglnargpheasptrpserleuleuasnglyglnserleu

385390395400

glyasnvalleuleualaglyglyleuglyalaaspasncysvalglu

405410415

alaalaglnthrglycysalaglyleuasppheasnseralavalglu

420425430

serglnproglyilelysaspalaargleuleualaservalphegln

435440445

thrleuargalatyr

450

<210>17

<211>1596

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>17

atggctgacattctgctgctcgataatatcgactcttttacgtacaacctggcagatcag60

ttgcgcagcaatgggcataacgtggtgatttaccgcaaccatattccggcgcaaacctta120

attgaacgcctggcgaccatgagcaatccggtgctgatgctttctcctggccccggtgtg180

ccgagcgaagccggttgtatgccggaactcctcacccgcttgcgtggcaagctgcccatt240

attggcatttgcctcggacatcaggcgattgtcgaagcttacgggggctatgtcggtcag300

gcgggcgaaattctccacggtaaagcctccagcattgaacatgacggtcaggcgatgttt360

gccggattaacaaacccgctgccggtggcgcgttatcactcgctggttggcagtaacatt420

ccggccggtttaaccatcaacgcccattttaatggcatggtgatggcagtacgtcacgat480

gcggatcgcgtttgtggattccagttccatccggaatccattctcaccacccagggcgct540

cgcctgctggaacaaacgctggcctgggcgcagcagaaactagagccagccaacacgctg600

caaccgattctggaaaaactgtatcaggcgcagacgcttagccaacaagaaagccaccag660

ctgttttcagcggtggtgcgtggcgagctgaagccggaacaactggcggcggcgctggtg720

agcatgaaaattcgcggtgagcacccgaacgagatcgccggggcagcaaccgcgctactg780

gaaaacgcagcgccgttcccgcgcccggattatctgtttgctgatatcgtcggtactggc840

ggtgacggcagcaacagtatcaatatttctaccgccagtgcgtttgtcgccgcggcctgt900

gggctgaaagtggcgaaacacggcaaccgtagcgtctccagtaaatctggttcgtccgat960

ctgctggcggcgttcggtattaatcttgatatgaacgccgataaatcgcgccaggcgctg1020

gatgagttaggtgtatgtttcctctttgcgccgaagtatcacaccggattccgccacgcg1080

atgccggttcgccagcaactgaaaacccgcaccctgttcaatgtgctggggccattgatt1140

aacccggcgcatccgccgctggcgttaattggtgtttatagtccggaactggtgctgccg1200

attgccgaaaccttgcgcgtgctggggtatcaacgcgcggcggtggtgcacagcggcggg1260

atggatgaagtttcattacacgcgccgacaatcgttgccgaactgcatgacggcgaaatt1320

aaaagctatcagctcaccgcagaagactttggcctgacaccctaccaccaggagcaactg1380

gcaggcggaacaccggaagaaaaccgtgacattttaacacgtttgttacaaggtaaaggc1440

gacgccgcccatgaagcagccgtcgctgcgaacgtcgccatgttaatgcgcctgcatggc1500

catgaagatctgcaagccaatgcgcaaaccgttcttgaggtactgcgcagtggttccgct1560

tacgacagagtcaccgcactggcggcacgagggtaa1596

<210>18

<211>531

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>18

metalaaspileleuleuleuaspasnileaspserphethrtyrasn

151015

leualaaspglnleuargserasnglyhisasnvalvaliletyrarg

202530

asnhisileproalaglnthrleuilegluargleualathrmetser

354045

asnprovalleumetleuserproglyproglyvalprosergluala

505560

glycysmetprogluleuleuthrargleuargglylysleuproile

65707580

ileglyilecysleuglyhisglnalailevalglualatyrglygly

859095

tyrvalglyglnalaglygluileleuhisglylysalaserserile

100105110

gluhisaspglyglnalametphealaglyleuthrasnproleupro

115120125

valalaargtyrhisserleuvalglyserasnileproalaglyleu

130135140

thrileasnalahispheasnglymetvalmetalavalarghisasp

145150155160

alaaspargvalcysglypheglnphehisprogluserileleuthr

165170175

thrglnglyalaargleuleugluglnthrleualatrpalaglngln

180185190

lysleugluproalaasnthrleuglnproileleuglulysleutyr

195200205

glnalaglnthrleuserglnglngluserhisglnleupheserala

210215220

valvalargglygluleulysprogluglnleualaalaalaleuval

225230235240

sermetlysileargglygluhisproasngluilealaglyalaala

245250255

thralaleuleugluasnalaalapropheproargproasptyrleu

260265270

phealaaspilevalglythrglyglyaspglyserasnserileasn

275280285

ileserthralaseralaphevalalaalaalacysglyleulysval

290295300

alalyshisglyasnargservalserserlysserglyserserasp

305310315320

leuleualaalapheglyileasnleuaspmetasnalaasplysser

325330335

argglnalaleuaspgluleuglyvalcyspheleuphealaprolys

340345350

tyrhisthrglyphearghisalametprovalargglnglnleulys

355360365

thrargthrleupheasnvalleuglyproleuileasnproalahis

370375380

proproleualaleuileglyvaltyrserprogluleuvalleupro

385390395400

ilealagluthrleuargvalleuglytyrglnargalaalavalval

405410415

hisserglyglymetaspgluvalserleuhisalaprothrileval

420425430

alagluleuhisaspglygluilelyssertyrglnleuthralaglu

435440445

asppheglyleuthrprotyrhisglngluglnleualaglyglythr

450455460

proglugluasnargaspileleuthrargleuleuglnglylysgly

465470475480

aspalaalahisglualaalavalalaalaasnvalalametleumet

485490495

argleuhisglyhisgluaspleuglnalaasnalaglnthrvalleu

500505510

gluvalleuargserglyseralatyraspargvalthralaleuala

515520525

alaarggly

530

<210>19

<211>1908

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>19

atggcacctgttacaattgaaaagttcgtaaatcaagaagaacgacaccttgtttccaac60

cgatcagcaacaattccgtttggtgaatacatatttaaaagattgttgtccatcgatacg120

aaatcagttttcggtgttcctggtgacttcaacttatctctattagaatatctctattca180

cctagtgttgaatcagctggcctaagatgggtcggcacgtgtaatgaactgaacgccgct240

tatgcggccgacggatattcccgttactctaataagattggctgtttaataaccacgtat300

ggcgttggtgaattaagcgccttgaacggtatagccggttcgttcgctgaaaatgtcaaa360

gttttgcacattgttggtgtggccaagtccatagattcgcgttcaagtaactttagtgat420

cggaacctacatcatttggtcccacagctacatgattcaaattttaaagggccaaatcat480

aaagtatatcatgatatggtaaaagatagagtcgcttgctcggtagcctacttggaggat540

attgaaactgcatgtgaccaagtcgataatgttatccgcgatatttacaagtattctaaa600

cctggttatatttttgttcctgcagattttgcggatatgtctgttacatgtgataatttg660

gttaatgttccacgtatatctcaacaagattgtatagtatacccttctgaaaaccaattg720

tctgacataatcaacaagattactagttggatatattccagtaaaacacctgcgatcctt780

ggagacgtactgactgataggtatggtgtgagtaactttttgaacaagcttatctgcaaa840

actgggatttggaatttttccactgttatgggaaaatctgtaattgatgagtcaaaccca900

acttatatgggtcaatataatggtaaagaaggtttaaaacaagtctatgaacattttgaa960

ctgtgcgacttggtcttgcattttggagtcgacatcaatgaaattaataatgggcattat1020

acttttacttataaaccaaatgctaaaatcattcaatttcatccgaattatattcgcctt1080

gtggacactaggcagggcaatgagcaaatgttcaaaggaatcaattttgcccctatttta1140

aaagaactatacaagcgcattgacgtttctaaactttctttgcaatatgattcaaatgta1200

actcaatatacgaacgaaacaatgcggttagaagatcctaccaatggacaatcaagcatt1260

attacacaagttcacttacaaaagacgatgcctaaatttttgaaccctggtgatgttgtc1320

gtttgtgaaacaggctcttttcaattctctgttcgtgatttcgcgtttccttcgcaatta1380

aaatatatatcgcaaggatttttcctttccattggcatggcccttcctgccgccctaggt1440

gttggaattgccatgcaagaccactcaaacgctcacatcaatggtggcaacgtaaaagag1500

gactataagccaagattaattttgtttgaaggtgacggtgcagcacagatgacaatccaa1560

gaactgagcaccattctgaagtgcaatattccactagaagttatcatttggaacaataac1620

ggctacactattgaaagagccatcatgggccctaccaggtcgtataacgacgttatgtct1680

tggaaatggaccaaactatttgaagcattcggagacttcgacggaaagtatactaatagc1740

actctcattcaatgtccctctaaattagcactgaaattggaggagcttaagaattcaaac1800

aaaagaagcgggatagaacttttagaagtcaaattaggcgaattggatttccccgaacag1860

ctaaagtgcatggttgaagcagcggcacttaaaagaaataaaaaatag1908

<210>20

<211>635

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>20

metalaprovalthrileglulysphevalasnglnglugluarghis

151015

leuvalserasnargseralathrilepropheglyglutyrilephe

202530

lysargleuleuserileaspthrlysservalpheglyvalprogly

354045

asppheasnleuserleuleuglutyrleutyrserproservalglu

505560

seralaglyleuargtrpvalglythrcysasngluleuasnalaala

65707580

tyralaalaaspglytyrserargtyrserasnlysileglycysleu

859095

ilethrthrtyrglyvalglygluleuseralaleuasnglyileala

100105110

glyserphealagluasnvallysvalleuhisilevalglyvalala

115120125

lysserileaspserargserserasnpheseraspargasnleuhis

130135140

hisleuvalproglnleuhisaspserasnphelysglyproasnhis

145150155160

lysvaltyrhisaspmetvallysaspargvalalacysservalala

165170175

tyrleugluaspilegluthralacysaspglnvalaspasnvalile

180185190

argaspiletyrlystyrserlysproglytyrilephevalproala

195200205

aspphealaaspmetservalthrcysaspasnleuvalasnvalpro

210215220

argileserglnglnaspcysilevaltyrprosergluasnglnleu

225230235240

seraspileileasnlysilethrsertrpiletyrserserlysthr

245250255

proalaileleuglyaspvalleuthraspargtyrglyvalserasn

260265270

pheleuasnlysleuilecyslysthrglyiletrpasnpheserthr

275280285

valmetglylysservalileaspgluserasnprothrtyrmetgly

290295300

glntyrasnglylysgluglyleulysglnvaltyrgluhispheglu

305310315320

leucysaspleuvalleuhispheglyvalaspileasngluileasn

325330335

asnglyhistyrthrphethrtyrlysproasnalalysileilegln

340345350

phehisproasntyrileargleuvalaspthrargglnglyasnglu

355360365

glnmetphelysglyileasnphealaproileleulysgluleutyr

370375380

lysargileaspvalserlysleuserleuglntyraspserasnval

385390395400

thrglntyrthrasngluthrmetargleugluaspprothrasngly

405410415

glnserserileilethrglnvalhisleuglnlysthrmetprolys

420425430

pheleuasnproglyaspvalvalvalcysgluthrglyserphegln

435440445

pheservalargaspphealapheproserglnleulystyrileser

450455460

glnglyphepheleuserileglymetalaleuproalaalaleugly

465470475480

valglyilealametglnasphisserasnalahisileasnglygly

485490495

asnvallysgluasptyrlysproargleuileleuphegluglyasp

500505510

glyalaalaglnmetthrileglngluleuserthrileleulyscys

515520525

asnileproleugluvalileiletrpasnasnasnglytyrthrile

530535540

gluargalailemetglyprothrargsertyrasnaspvalmetser

545550555560

trplystrpthrlysleupheglualapheglyasppheaspglylys

565570575

tyrthrasnserthrleuileglncysproserlysleualaleulys

580585590

leuglugluleulysasnserasnlysargserglyilegluleuleu

595600605

gluvallysleuglygluleuasppheprogluglnleulyscysmet

610615620

valglualaalaalaleulysargasnlyslys

625630635

<210>21

<211>1488

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>21

atgaattttcatcatctggcttactggcaggataaagcgttaagtctcgccattgaaaac60

cgcttatttattaacggtgaatatactgctgcggcggaaaatgaaacctttgaaaccgtt120

gatccggtcacccaggcaccgctggcgaaaattgcccgcggcaagagcgtcgatatcgac180

cgtgcgatgagcgcagcacgcggcgtatttgaacgcggcgactggtcactctcttctccg240

gctaaacgtaaagcggtactgaataaactcgccgatttaatggaagcccacgccgaagag300

ctggcactgctggaaactctcgacaccggcaaaccgattcgtcacagtctgcgtgatgat360

attcccggcgcggcgcgcgccattcgctggtacgccgaagcgatcgacaaagtgtatggc420

gaagtggcgaccaccagtagccatgagctggcgatgatcgtgcgtgaaccggtcggcgtg480

attgccgccatcgtgccgtggaacttcccgctgttgctgacttgctggaaactcggcccg540

gcgctggcggcgggaaacagcgtgattctaaaaccgtctgaaaaatcaccgctcagtgcg600

attcgtctcgcggggctggcgaaagaagcaggcttgccggatggtgtgttgaacgtggtg660

acgggttttggtcatgaagccgggcaggcgctgtcgcgtcataacgatatcgacgccatt720

gcctttaccggttcaacccgtaccgggaaacagctgctgaaagatgcgggcgacagcaac780

atgaaacgcgtctggctggaagcgggcggcaaaagcgccaacatcgttttcgctgactgc840

ccggatttgcaacaggcggcaagcgccaccgcagcaggcattttctacaaccagggacag900

gtgtgcatcgccggaacgcgcctgttgctggaagagagcatcgccgatgaattcttagcc960

ctgttaaaacagcaggcgcaaaactggcagccgggccatccacttgatcccgcaaccacc1020

atgggcaccttaatcgactgcgcccacgccgactcggtccatagctttattcgggaaggc1080

gaaagcaaagggcaactgttgttggatggccgtaacgccgggctggctgccgccatcggc1140

ccgaccatctttgtggatgtggacccgaatgcgtccttaagtcgcgaagagattttcggt1200

ccggtgctggtggtcacgcgtttcacatcagaagaacaggcgctacagcttgccaacgac1260

agccagtacggccttggcgcggcggtatggacgcgcgacctctcccgcgcgcaccgcatg1320

agccgacgcctgaaagccggttccgtcttcgtcaataactacaacgacggcgatatgacc1380

gtgccgtttggcggctataagcagagcggcaacggtcgcgacaaatccctgcatgccctt1440

gaaaaattcactgaactgaaaaccatctggataagcctggaggcctga1488

<210>22

<211>495

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>22

metasnphehishisleualatyrtrpglnasplysalaleuserleu

151015

alailegluasnargleupheileasnglyglutyrthralaalaala

202530

gluasngluthrphegluthrvalaspprovalthrglnalaproleu

354045

alalysilealaargglylysservalaspileaspargalametser

505560

alaalaargglyvalphegluargglyasptrpserleuserserpro

65707580

alalysarglysalavalleuasnlysleualaaspleumetgluala

859095

hisalaglugluleualaleuleugluthrleuaspthrglylyspro

100105110

ilearghisserleuargaspaspileproglyalaalaargalaile

115120125

argtrptyralaglualaileasplysvaltyrglygluvalalathr

130135140

thrserserhisgluleualametilevalarggluprovalglyval

145150155160

ilealaalailevalprotrpasnpheproleuleuleuthrcystrp

165170175

lysleuglyproalaleualaalaglyasnservalileleulyspro

180185190

serglulysserproleuseralaileargleualaglyleualalys

195200205

glualaglyleuproaspglyvalleuasnvalvalthrglyphegly

210215220

hisglualaglyglnalaleuserarghisasnaspileaspalaile

225230235240

alaphethrglyserthrargthrglylysglnleuleulysaspala

245250255

glyaspserasnmetlysargvaltrpleuglualaglyglylysser

260265270

alaasnilevalphealaaspcysproaspleuglnglnalaalaser

275280285

alathralaalaglyilephetyrasnglnglyglnvalcysileala

290295300

glythrargleuleuleuglugluserilealaaspglupheleuala

305310315320

leuleulysglnglnalaglnasntrpglnproglyhisproleuasp

325330335

proalathrthrmetglythrleuileaspcysalahisalaaspser

340345350

valhisserpheilearggluglygluserlysglyglnleuleuleu

355360365

aspglyargasnalaglyleualaalaalaileglyprothrilephe

370375380

valaspvalaspproasnalaserleuserargglugluilephegly

385390395400

provalleuvalvalthrargphethrserglugluglnalaleugln

405410415

leualaasnaspserglntyrglyleuglyalaalavaltrpthrarg

420425430

aspleuserargalahisargmetserargargleulysalaglyser

435440445

valphevalasnasntyrasnaspglyaspmetthrvalprophegly

450455460

glytyrlysglnserglyasnglyargasplysserleuhisalaleu

465470475480

glulysphethrgluleulysthriletrpileserleugluala

485490495

<210>23

<211>1503

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>23

atgactttacctgaatcaaaagacttttcttacttgttttcggatgaaaccaatgctcgt60

aaaccatccccattgaaaacctgcatccatcttttccaagatcctaacattatctttttg120

ggtggtggcctgccattaaaagattatttcccatgggataatctatctgtagattcaccc180

aagcctccttttccccagggtattggagctccaattgacgagcagaattgcataaaatac240

accgtcaacaaagattacgctgataaaagtgccaatccttccaacgatattcctttgtca300

agagctttgcaatacgggttcagtgctggtcaacctgaactattaaacttcattagagat360

cataccaagattatccacgatttgaagtataaggactgggacgttttagccactgcaggt420

aacacaaatgcctgggaatctactttaagagtcttttgtaaccgaggtgatgtcatctta480

gttgaggcacattctttttcctcttcattggcttctgcagaggctcaaggtgtcattacc540

ttccccgtgccaattgacgctgatggtatcattcctgaaaaattagctaaagtcatggaa600

aactggacacctggtgctcctaaaccaaagttgttatacactattccaacgggccaaaat660

ccaactggtacttccattgcagaccatagaaaggaggcaatttacaagatcgctcaaaag720

tacgacttcctaattgtggaagatgaaccttattatttcttacaaatgaatccctacatc780

aaagacttgaaggaaagagagaaggcacaaagttctccaaagcaggaccatgacgaattt840

ttgaagtccttggcaaacactttcctttccttggatacagaaggccgtgttattagaatg900

gattccttttcaaaagttttggccccagggacaagattgggttggattactggttcatcc960

aaaatcttgaagccttacttgagtttgcatgaaatgacgattcaagccccagcaggtttt1020

acacaagttttggtcaacgctacgctatccaggtggggtcaaaagggttacttggactgg1080

ttgcttggcctgcgtcatgaatacactttgaaacgtgactgtgccatcgatgccctttac1140

aagtatctaccacaatctgatgctttcgtgatcaatcctccaattgcaggtatgtttttc1200

accgtgaacattgacgcatctgtccaccctgagtttaaaacaaaatacaactcagaccct1260

taccagctagaacagagtctttaccacaaagtggttgaacgtggtgttttagtggttccc1320

ggttcttggttcaagagtgagggtgagacggaacctcctcaacccgctgaatctaaagaa1380

gtcagtaatccaaacataattttcttcagaggtacctatgcagctgtctctcctgagaaa1440

ctgactgaaggtctgaagagattaggtgatactttatacgaagaatttggtatttccaaa1500

tag1503

<210>24

<211>500

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>24

metthrleuprogluserlysaspphesertyrleupheseraspglu

151015

thrasnalaarglysproserproleulysthrcysilehisleuphe

202530

glnaspproasnileilepheleuglyglyglyleuproleulysasp

354045

tyrpheprotrpaspasnleuservalaspserprolysproprophe

505560

proglnglyileglyalaproileaspgluglnasncysilelystyr

65707580

thrvalasnlysasptyralaasplysseralaasnproserasnasp

859095

ileproleuserargalaleuglntyrglypheseralaglyglnpro

100105110

gluleuleuasnpheileargasphisthrlysileilehisaspleu

115120125

lystyrlysasptrpaspvalleualathralaglyasnthrasnala

130135140

trpgluserthrleuargvalphecysasnargglyaspvalileleu

145150155160

valglualahisserpheserserserleualaseralaglualagln

165170175

glyvalilethrpheprovalproileaspalaaspglyileilepro

180185190

glulysleualalysvalmetgluasntrpthrproglyalaprolys

195200205

prolysleuleutyrthrileprothrglyglnasnprothrglythr

210215220

serilealaasphisarglysglualailetyrlysilealaglnlys

225230235240

tyrasppheleuilevalgluaspgluprotyrtyrpheleuglnmet

245250255

asnprotyrilelysaspleulysgluargglulysalaglnserser

260265270

prolysglnasphisaspglupheleulysserleualaasnthrphe

275280285

leuserleuaspthrgluglyargvalileargmetaspserpheser

290295300

lysvalleualaproglythrargleuglytrpilethrglyserser

305310315320

lysileleulysprotyrleuserleuhisglumetthrileglnala

325330335

proalaglyphethrglnvalleuvalasnalathrleuserargtrp

340345350

glyglnlysglytyrleuasptrpleuleuglyleuarghisglutyr

355360365

thrleulysargaspcysalaileaspalaleutyrlystyrleupro

370375380

glnseraspalaphevalileasnproproilealaglymetphephe

385390395400

thrvalasnileaspalaservalhisprogluphelysthrlystyr

405410415

asnseraspprotyrglnleugluglnserleutyrhislysvalval

420425430

gluargglyvalleuvalvalproglysertrpphelysserglugly

435440445

gluthrgluproproglnproalagluserlysgluvalserasnpro

450455460

asnileilephepheargglythrtyralaalavalserproglulys

465470475480

leuthrgluglyleulysargleuglyaspthrleutyrglugluphe

485490495

glyileserlys

500

<210>25

<211>327

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>25

atggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggtta60

cgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaac120

ctgatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagag180

ctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcggcgcaggcatcgcg240

acgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctg300

gaagaggtgttgctgaaaagcgattga327

<210>26

<211>108

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>26

metalaglnglnserprotyrseralaalametalagluglnarghis

151015

glnglutrpleuargphevalaspleuleulysasnalatyrglnasn

202530

aspleuhisleuproleuleuasnleumetleuthrproaspgluarg

354045

glualaleuglythrargvalargilevalglugluleuleuarggly

505560

glumetserglnarggluleulysasngluleuglyalaglyileala

65707580

thrilethrargglyserasnserleulysalaalaprovalgluleu

859095

argglntrpleuglugluvalleuleulysserasp

100105

<210>27

<211>804

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>27

atggctatccctgcatttggtttaggtactttccgtctgaaagacgacgttgttatttca60

tctgtgataacggcgcttgaacttggttatcgcgcaattgataccgcacaaatctatgat120

aacgaagccgcagtaggtcaggcgattgcagaaagtggcgtgccacgtcatgaactctac180

atcaccactaaaatctggattgaaaatctcagcaaagacaaattgatcccaagtctgaaa240

gagagcctgcaaaaattgcgtaccgattatgttgatctgacgctaatccactggccgtca300

ccaaacgatgaagtctctgttgaagagtttatgcaggcgctgctggaagccaaaaaacaa360

gggctgacgcgtgagatcggtatttccaacttcacgatcccgttgatggaaaaagcgatt420

gctgctgttggtgctgaaaacatcgctactaaccagattgaactctctccttatctgcaa480

aaccgtaaagtggttgcctgggctaaacagcacggcatccatattacttcctatatgacg540

ctggcgtatggtaaggccctgaaagatgaggttattgctcgtatcgcagctaaacacaat600

gcgactccggcacaagtgattctggcgtgggctatgggggaaggttactcagtaattcct660

tcttctactaaacgtaaaaacctggaaagtaatcttaaggcacaaaatttacagcttgat720

gccgaagataaaaaagcgatcgccgcactggattgcaacgaccgcctggttagcccggaa780

ggtctggctcctgaatgggattaa804

<210>28

<211>267

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>28

metalaileproalapheglyleuglythrpheargleulysaspasp

151015

valvalileserservalilethralaleugluleuglytyrargala

202530

ileaspthralaglniletyraspasnglualaalavalglyglnala

354045

ilealagluserglyvalproarghisgluleutyrilethrthrlys

505560

iletrpilegluasnleuserlysasplysleuileproserleulys

65707580

gluserleuglnlysleuargthrasptyrvalaspleuthrleuile

859095

histrpproserproasnaspgluvalservalglugluphemetgln

100105110

alaleuleuglualalyslysglnglyleuthrarggluileglyile

115120125

serasnphethrileproleumetglulysalailealaalavalgly

130135140

alagluasnilealathrasnglnilegluleuserprotyrleugln

145150155160

asnarglysvalvalalatrpalalysglnhisglyilehisilethr

165170175

sertyrmetthrleualatyrglylysalaleulysaspgluvalile

180185190

alaargilealaalalyshisasnalathrproalaglnvalileleu

195200205

alatrpalametglygluglytyrservalileproserserthrlys

210215220

arglysasnleugluserasnleulysalaglnasnleuglnleuasp

225230235240

alagluasplyslysalailealaalaleuaspcysasnaspargleu

245250255

valserprogluglyleualaproglutrpasp

260265

<210>29

<211>855

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>29

atgcaacaaaaaatgattcaatttagtggcgatgtctcactgccagccgtagggcaggga60

acatggtatatgggcgaagatgccagtcagcgcaaaacagaagttgctgcactacgcgcg120

ggcattgaactcggtttaaccctcattgataccgccgaaatgtatgccgatggcggtgcc180

gaaaaggtggttggggaagcattaaccggtctgcgagagaaggtctttctcgtctctaaa240

gtctatccgtggaatgctggcgggcaaaaagcgataaatgcatgcgaagccagtttacgc300

cgtctcaatactgattatctcgatctttacttattacactggtctggcagtttcgctttt360

gaagagactgtcgcagcgatggaaaaattgatcgcccagggaaaaatccgccgctggggc420

gtttctaaccttgattatgctgatatgcaggaactctggcagctgccggggggaaatcag480

tgtgccactaatcaggtgctttaccatctcggttcacgaggaattgagtacgatctactc540

ccctggtgccagcaacagcagatgccggtgatggcttacagtccgttagcccaggccggg600

cggttgcgcaatggactgttaaaaaacgcggtagtcaacgaaattgcacatgctcacaat660

atcagcgcggcacaagtattgttggcgtgggtgatcagtcatcagggtgtgatggcgatt720

ccaaaagcggccacgattgcccatgtccaacaaaatgcggctgtgcttgaggtcgaactt780

tcttcagcggaattagctatgctggataaggcatatccggcaccaaaaggaaaaactgcg840

ctggatatggtgtga855

<210>30

<211>284

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>30

metglnglnlysmetileglnpheserglyaspvalserleuproala

151015

valglyglnglythrtrptyrmetglygluaspalaserglnarglys

202530

thrgluvalalaalaleuargalaglyilegluleuglyleuthrleu

354045

ileaspthralaglumettyralaaspglyglyalaglulysvalval

505560

glyglualaleuthrglyleuargglulysvalpheleuvalserlys

65707580

valtyrprotrpasnalaglyglyglnlysalaileasnalacysglu

859095

alaserleuargargleuasnthrasptyrleuaspleutyrleuleu

100105110

histrpserglyserphealapheglugluthrvalalaalametglu

115120125

lysleuilealaglnglylysileargargtrpglyvalserasnleu

130135140

asptyralaaspmetglngluleutrpglnleuproglyglyasngln

145150155160

cysalathrasnglnvalleutyrhisleuglyserargglyileglu

165170175

tyraspleuleuprotrpcysglnglnglnglnmetprovalmetala

180185190

tyrserproleualaglnalaglyargleuargasnglyleuleulys

195200205

asnalavalvalasngluilealahisalahisasnileseralaala

210215220

glnvalleuleualatrpvalileserhisglnglyvalmetalaile

225230235240

prolysalaalathrilealahisvalglnglnasnalaalavalleu

245250255

gluvalgluleuserseralagluleualametleuasplysalatyr

260265270

proalaprolysglylysthralaleuaspmetval

275280

<210>31

<211>957

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>31

atgctacaaaattgcgcacaatcaaattgccgcattattcctaagaaattacgcgatatg60

aaacgtgaagagatttgccgcttgctggcggataaagttaataaactgaaaaataaagaa120

aatagtttgtcaggactgttgcccgatgtgcgtttgttgtatggcgagacgcctttcgca180

cgtacaccggtgatgtacgagcctggcatcataattctcttttccgggcataaaatcggt240

tatatcaatgaacgcgtgtttcgttatgatgccaatgaatacctgctgctgacggtgccg300

ttgccgtttgagtgcgaaacctatgccacgtcagaggtgccgctggcagggttgcgtctc360

aatgtcgatattttgcagttacaggaactgttgatggacattggcgaagatgagcatttc420

cagccgtcgatggcagccagcgggattaactccgccacgttatcagaagagattttatgc480

gcggcggagcggttactcgacgtgatggagcgaccactggatgcgcgtattctcggcaaa540

cagatcatccgcgaaattctgtactacgtgctgaccggaccttgcggcggcgcgttactg600

gcgctggtcagtcgccagactcacttcagtctgattagccgcgtgctgaaacggattgag660

aataaatacaccgaaaacctgagcgtcgagcaactggcggcagaagccaacatgagcgta720

tcggcgttccaccataattttaagtctgtcaccagtacctcgccgttgcagtatttgaag780

aattaccgtctgcataaggcgcggatgatgatcatccatgacggcatgaaggccagcgca840

gcagcgatgcgcgtcggctatgaaagcgcatcgcaatttagccgtgagtttaaacgttac900

ttcggtgtgacgccgggggaagatgcggcaagaatgcgggcgatgcaggggaattaa957

<210>32

<211>318

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>32

metleuglnasncysalaglnserasncysargileileprolyslys

151015

leuargaspmetlysargglugluilecysargleuleualaasplys

202530

valasnlysleulysasnlysgluasnserleuserglyleuleupro

354045

aspvalargleuleutyrglygluthrprophealaargthrproval

505560

mettyrgluproglyileileileleupheserglyhislysilegly

65707580

tyrileasngluargvalpheargtyraspalaasnglutyrleuleu

859095

leuthrvalproleupropheglucysgluthrtyralathrserglu

100105110

valproleualaglyleuargleuasnvalaspileleuglnleugln

115120125

gluleuleumetaspileglygluaspgluhispheglnprosermet

130135140

alaalaserglyileasnseralathrleuserglugluileleucys

145150155160

alaalagluargleuleuaspvalmetgluargproleuaspalaarg

165170175

ileleuglylysglnileilearggluileleutyrtyrvalleuthr

180185190

glyprocysglyglyalaleuleualaleuvalserargglnthrhis

195200205

pheserleuileserargvalleulysargilegluasnlystyrthr

210215220

gluasnleuservalgluglnleualaalaglualaasnmetserval

225230235240

seralaphehishisasnphelysservalthrserthrserproleu

245250255

glntyrleulysasntyrargleuhislysalaargmetmetileile

260265270

hisaspglymetlysalaseralaalaalametargvalglytyrglu

275280285

seralaserglnpheserarggluphelysargtyrpheglyvalthr

290295300

proglygluaspalaalaargmetargalametglnglyasn

305310315

<210>33

<211>1164

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>33

atgaacaactttaatctgcacaccccaacccgcattctgtttggtaaaggcgcaatcgct60

ggtttacgcgaacaaattcctcacgatgctcgcgtattgattacctacggcggcggcagc120

gtgaaaaaaaccggcgttctcgatcaagttctggatgccctgaaaggcatggacgtgctg180

gaatttggcggtattgagccaaacccggcttatgaaacgctgatgaacgccgtgaaactg240

gttcgcgaacagaaagtgactttcctgctggcggttggcggcggttctgtactggacggc300

accaaatttatcgccgcagcggctaactatccggaaaatatcgatccgtggcacattctg360

caaacgggcggtaaagagattaaaagcgccatcccgatgggctgtgtgctgacgctgcca420

gcaaccggttcagaatccaacgcaggcgcggtgatctcccgtaaaaccacaggcgacaag480

caggcgttccattctgcccatgttcagccggtatttgccgtgctcgatccggtttatacc540

tacaccctgccgccgcgtcaggtggctaacggcgtagtggacgcctttgtacacaccgtg600

gaacagtatgttaccaaaccggttgatgccaaaattcaggaccgtttcgcagaaggcatt660

ttgctgacgctaatcgaagatggtccgaaagccctgaaagagccagaaaactacgatgtg720

cgcgccaacgtcatgtgggcggcgactcaggcgctgaacggtttgattggcgctggcgta780

ccgcaggactgggcaacgcatatgctgggccacgaactgactgcgatgcacggtctggat840

cacgcgcaaacactggctatcgtcctgcctgcactgtggaatgaaaaacgcgataccaag900

cgcgctaagctgctgcaatatgctgaacgcgtctggaacatcactgaaggttccgatgat960

gagcgtattgacgccgcgattgccgcaacccgcaatttctttgagcaattaggcgtgccg1020

acccacctctccgactacggtctggacggcagctccatcccggctttgctgaaaaaactg1080

gaagagcacggcatgacccaactgggcgaaaatcatgacattacgttggatgtcagccgc1140

cgtatatacgaagccgcccgctaa1164

<210>34

<211>387

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>34

metasnasnpheasnleuhisthrprothrargileleupheglylys

151015

glyalailealaglyleuarggluglnileprohisaspalaargval

202530

leuilethrtyrglyglyglyservallyslysthrglyvalleuasp

354045

glnvalleuaspalaleulysglymetaspvalleuglupheglygly

505560

ilegluproasnproalatyrgluthrleumetasnalavallysleu

65707580

valarggluglnlysvalthrpheleuleualavalglyglyglyser

859095

valleuaspglythrlyspheilealaalaalaalaasntyrproglu

100105110

asnileaspprotrphisileleuglnthrglyglylysgluilelys

115120125

seralaileprometglycysvalleuthrleuproalathrglyser

130135140

gluserasnalaglyalavalileserarglysthrthrglyasplys

145150155160

glnalaphehisseralahisvalglnprovalphealavalleuasp

165170175

provaltyrthrtyrthrleuproproargglnvalalaasnglyval

180185190

valaspalaphevalhisthrvalgluglntyrvalthrlysproval

195200205

aspalalysileglnaspargphealagluglyileleuleuthrleu

210215220

ilegluaspglyprolysalaleulysgluprogluasntyraspval

225230235240

argalaasnvalmettrpalaalathrglnalaleuasnglyleuile

245250255

glyalaglyvalproglnasptrpalathrhismetleuglyhisglu

260265270

leuthralamethisglyleuasphisalaglnthrleualaileval

275280285

leuproalaleutrpasnglulysargaspthrlysargalalysleu

290295300

leuglntyralagluargvaltrpasnilethrgluglyseraspasp

305310315320

gluargileaspalaalailealaalathrargasnphepheglugln

325330335

leuglyvalprothrhisleuserasptyrglyleuaspglyserser

340345350

ileproalaleuleulyslysleuglugluhisglymetthrglnleu

355360365

glygluasnhisaspilethrleuaspvalserargargiletyrglu

370375380

alaalaarg

385

<210>35

<211>828

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>35

atggctaatccaaccgttattaagctacaggatggcaatgtcatgccccagctgggactg60

ggcgtctggcaagcaagtaatgaggaagtaatcaccgccattcaaaaagcgttagaagtg120

ggttatcgctcgattgataccgccgcggcctacaagaacgaagaaggtgtcggcaaagcc180

ctgaaaaatgcctcagtcaacagagaagaactgttcatcaccactaagctgtggaacgac240

gaccacaagcgcccccgcgaagccctgctcgacagcctgaaaaaactccagcttgattat300

atcgacctctacttaatgcactggcccgttcccgctatcgaccattatgtcgaagcatgg360

aaaggcatgatcgaattgcaaaaagagggattaatcaaaagcatcggcgtgtgcaacttc420

cagatccatcacctgcaacgcctgattgatgaaactggcgtgacgcctgtgataaaccag480

atcgaacttcatccgctgatgcaacaacgccagctacacgcctggaacgcgacacacaaa540

atccagaccgaatcctggagcccattagcgcaaggagggaaaggcgttttcgatcagaaa600

gtcattcgcgatctggcagataaatacggcaaaaccccggcgcagattgttatccgctgg660

catctggatagcggcctggtggtgatcccgaaatcggtcacaccttcacgtattgccgaa720

aactttgatgtctgggatttccgtctcgacaaagacgaactcggcgaaattgcaaaactc780

gatcagggcaagcgtctcggtcccgatcctgaccagttcggcggctaa828

<210>36

<211>275

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>36

metalaasnprothrvalilelysleuglnaspglyasnvalmetpro

151015

glnleuglyleuglyvaltrpglnalaserasnglugluvalilethr

202530

alaileglnlysalaleugluvalglytyrargserileaspthrala

354045

alaalatyrlysasnglugluglyvalglylysalaleulysasnala

505560

servalasnargglugluleupheilethrthrlysleutrpasnasp

65707580

asphislysargproargglualaleuleuaspserleulyslysleu

859095

glnleuasptyrileaspleutyrleumethistrpprovalproala

100105110

ileasphistyrvalglualatrplysglymetilegluleuglnlys

115120125

gluglyleuilelysserileglyvalcysasnpheglnilehishis

130135140

leuglnargleuileaspgluthrglyvalthrprovalileasngln

145150155160

ilegluleuhisproleumetglnglnargglnleuhisalatrpasn

165170175

alathrhislysileglnthrglusertrpserproleualaglngly

180185190

glylysglyvalpheaspglnlysvalileargaspleualaasplys

195200205

tyrglylysthrproalaglnilevalileargtrphisleuaspser

210215220

glyleuvalvalileprolysservalthrproserargilealaglu

225230235240

asnpheaspvaltrpasppheargleuasplysaspgluleuglyglu

245250255

ilealalysleuaspglnglylysargleuglyproaspproaspgln

260265270

pheglygly

275

<210>37

<211>1050

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>37

atgaagatcaaagctgttggtgcatattccgctaaacaaccacttgaaccgatggatatc60

acccggcgtgaaccgggaccgaatgatgtcaaaatcgaaatcgcttactgtggcgtttgc120

cattccgatctccaccaggtccgttccgagtgggcggggacggtttacccctgcgtgccg180

ggtcatgaaattgtggggcgtgtggtagccgttggtgatcaggtagaaaaatatgcgccg240

ggcgatctggtcggtgtcggctgcattgtcgacagttgtaaacattgcgaagagtgtgaa300

gacgggttggaaaactactgtgatcacatgaccggcacctataactcgccgacgccggac360

gaaccgggccatactctgggcggctactcacaacagatcgtcgttcatgagcgatatgtt420

ctgcgtattcgtcacccgcaagagcagctggcggcggtggctcctttgttgtgtgcaggg480

atcaccacgtattcgccgctacgtcactggcaggccgggccgggtaaaaaagtgggcgtg540

gtcggcatcggcggtctgggacatatggggattaagctggcccacgcgatgggggcacat600

gtggtggcatttaccacttctgaggcaaaacgcgaagcggcaaaagccctgggggccgat660

gaagttgttaactcacgcaatgccgatgagatggcggctcatctgaagagtttcgatttc720

attttgaatacagtagctgcgccacataatctcgacgattttaccaccttgctgaagcgt780

gatggcaccatgacgctggttggtgcgcctgcgacaccgcataaatcgccggaagttttc840

aacctgatcatgaaacgccgtgcgatagccggttctatgattggcggcattccagaaact900

caggagatgctcgatttttgcgccgaacatggcatcgtggctgatatagagatgattcgg960

gccgatcaaattaatgaagcctatgagcgaatgctgcgcggtgatgtgaaatatcgtttt1020

gttatcgataatcgcacactaacagactga1050

<210>38

<211>349

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>38

metlysilelysalavalglyalatyrseralalysglnproleuglu

151015

prometaspilethrargarggluproglyproasnaspvallysile

202530

gluilealatyrcysglyvalcyshisseraspleuhisglnvalarg

354045

serglutrpalaglythrvaltyrprocysvalproglyhisgluile

505560

valglyargvalvalalavalglyaspglnvalglulystyralapro

65707580

glyaspleuvalglyvalglycysilevalaspsercyslyshiscys

859095

gluglucysgluaspglyleugluasntyrcysasphismetthrgly

100105110

thrtyrasnserprothrproaspgluproglyhisthrleuglygly

115120125

tyrserglnglnilevalvalhisgluargtyrvalleuargilearg

130135140

hisproglngluglnleualaalavalalaproleuleucysalagly

145150155160

ilethrthrtyrserproleuarghistrpglnalaglyproglylys

165170175

lysvalglyvalvalglyileglyglyleuglyhismetglyilelys

180185190

leualahisalametglyalahisvalvalalaphethrthrserglu

195200205

alalysargglualaalalysalaleuglyalaaspgluvalvalasn

210215220

serargasnalaaspglumetalaalahisleulysserpheaspphe

225230235240

ileleuasnthrvalalaalaprohisasnleuaspaspphethrthr

245250255

leuleulysargaspglythrmetthrleuvalglyalaproalathr

260265270

prohislysserprogluvalpheasnleuilemetlysargargala

275280285

ilealaglysermetileglyglyileprogluthrglnglumetleu

290295300

aspphecysalagluhisglyilevalalaaspileglumetilearg

305310315320

alaaspglnileasnglualatyrgluargmetleuargglyaspval

325330335

lystyrargphevalileaspasnargthrleuthrasp

340345

<210>39

<211>1020

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>39

atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgaactggaagtttatgag60

tacgatcccggtgagctgaggccacaagatgttgaagtgcaggtggattactgcgggatc120

tgccattccgatctgtcgatgatcgataacgaatggggattttcacaatatccgctggtt180

gccgggcatgaggtgattgggcgcgtggtggcactcgggagcgccgcgcaggataaaggt240

ttgcaggtcggtcagcgtgtcgggattggctggacggcgcgtagctgtggtcactgcgac300

gcctgtattagcggtaatcagatcaactgcgagcaaggtgcggtgccgacgattatgaat360

cgcggtggctttgccgagaagttgcgtgcggactggcaatgggtgattccactgccagaa420

aatattgatatcgagtccgccgggccgctgttgtgcggcggtatcacggtctttaaacca480

ctgttgatgcaccatatcactgctaccagccgcgttggggtaattggtattggcgggctg540

gggcatatcgctataaaacttctgcacgcaatgggatgcgaggtgacagcctttagttct600

aatccggcgaaagagcaggaagtgctggcgatgggtgccgataaagtggtgaatagccgc660

gatccgcaggcactgaaagcactggcggggcagtttgatctcattatcaacaccgtcaac720

gtcagcctcgactggcagccctattttgaggcgctgacctatggcggtaatttccatacg780

gtcggtgcggttctcacgccgctgtctgttccggcctttacgttaattgcgggcgatcgc840

agcgtctctggttctgctaccggcacgccttatgagctgcgtaagctgatgcgttttgcc900

gcccgcagcaaggttgcgccgaccaccgaactgttcccgatgtcgaaaattaacgacgcc960

atccagcatgtgcgcgacggtaaggcgcgttaccgcgtggtgttgaaagccgatttttga1020

<210>40

<211>339

<212>prt

<213>2ambystomalateralexambystomajeffersonianum

<400>40

metsermetilelyssertyralaalalysglualaglyglygluleu

151015

gluvaltyrglutyraspproglygluleuargproglnaspvalglu

202530

valglnvalasptyrcysglyilecyshisseraspleusermetile

354045

aspasnglutrpglypheserglntyrproleuvalalaglyhisglu

505560

valileglyargvalvalalaleuglyseralaalaglnasplysgly

65707580

leuglnvalglyglnargvalglyileglytrpthralaargsercys

859095

glyhiscysaspalacysileserglyasnglnileasncysglugln

100105110

glyalavalprothrilemetasnargglyglyphealaglulysleu

115120125

argalaasptrpglntrpvalileproleuprogluasnileaspile

130135140

gluseralaglyproleuleucysglyglyilethrvalphelyspro

145150155160

leuleumethishisilethralathrserargvalglyvalilegly

165170175

ileglyglyleuglyhisilealailelysleuleuhisalametgly

180185190

cysgluvalthralapheserserasnproalalysgluglngluval

195200205

leualametglyalaasplysvalvalasnserargaspproglnala

210215220

leulysalaleualaglyglnpheaspleuileileasnthrvalasn

225230235240

valserleuasptrpglnprotyrpheglualaleuthrtyrglygly

245250255

asnphehisthrvalglyalavalleuthrproleuservalproala

260265270

phethrleuilealaglyaspargservalserglyseralathrgly

275280285

thrprotyrgluleuarglysleumetargphealaalaargserlys

290295300

valalaprothrthrgluleupheprometserlysileasnaspala

305310315320

ileglnhisvalargaspglylysalaargtyrargvalvalleulys

325330335

alaaspphe

<210>41

<211>62

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>41

atggcccaacaatcaccctattcagcagcgatggcagaacaggtgtaggctggagctgct60

tc62

<210>42

<211>60

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>42

tcaatcgcttttcagcaacacctcttccagccactggcgcacatatgaatatcctcctta60

<210>43

<211>60

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>43

atggctatccctgcatttggtttaggtactttccgtctgagtgtaggctggagctgcttc60

<210>44

<211>62

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>44

ttaatcccattcaggagccagaccttccgggctaaccaggcggcatatgaatatcctcct60

ta62

<210>45

<211>62

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>45

atgcaacaaaaaatgattcaatttagtggcgatgtctcactggtgtaggctggagctgct60

tc62

<210>46

<211>64

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>46

atgctacaaaattgcgcacaatcaaattgccgcattattcctaagtgtaggctggagctg60

cttc64

<210>47

<211>61

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>47

ttaattcccctgcatcgcccgcattcttgccgcatcttcccccatatgaatatcctcctt60

a61

<210>48

<211>61

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>48

atgaacaactttaatctgcacaccccaacccgcattctgttgtgtaggctggagctgctt60

c61

<210>49

<211>60

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>49

ttagcgggcggcttcgtatatacggcggctgacatccaacgcatatgaatatcctcctta60

<210>50

<211>62

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>50

atggctaatccaaccgttattaagctacaggatggcaatgtcgtgtaggctggagctgct60

tc62

<210>51

<211>60

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>51

ttagccgccgaactggtcaggatcgggaccgagacgcttgccatatgaatatcctcctta60

<210>52

<211>61

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>52

atgaagatcaaagctgttggtgcatattccgctaaacaaccgtgtaggctggagctgctt60

c61

<210>53

<211>60

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>53

tcagtctgttagtgtgcgattatcgataacaaaacgatattcatatgaatatcctcctta60

<210>54

<211>62

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>54

atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgtgtaggctggagctgct60

tc62

<210>55

<211>63

<212>dna

<213>2ambystomalateralexambystomajeffersonianum

<400>55

tcaaaaatcggctttcaacaccacgcggtaacgcgccttaccgtcatatgaatatcctcc60

tta63