胶原凝集素－补体激活蛋白嵌合体的制作方法

专利名称：胶原凝集素－补体激活蛋白嵌合体的制作方法
背景技术：
本发明涉及能够激活补体系统的融合蛋白，产生所述融合蛋白的方法以及包含所述融合蛋白的药物组合物，和用所述融合蛋白治疗疾病，特别是感染的方法。
背景技术：
动物已经发展了不同的复杂策略来保护它们对抗感染。免疫反应被分为两个主要的组，获得性免疫反应和先天免疫反应，前者中出现了适应并且细胞的作用占优势，后者可即时享用并且主要基于体液中存在的分子。先天免疫系统在出生时就可以运作(operational)，与此相比获得性免疫防御只在婴儿期获得其保护机体的全部能力(Janeway等，1999)。
在粘膜表面或通过破损皮肤进入机体的细菌迅速为胶原凝集素(collectin所识别，所述胶原凝集素是识别存在于微生物的表面但不在多细胞生物体细胞上的独特糖构型的可溶蛋白家族。因此胶原凝集素属于先天免疫系统的庞大并且多样的一组模式识别受体。人类已知三种胶原凝集素，然而可能存在其它的胶原凝集素，例如牛中就有更多。胶原凝集素靶向粒子，并与粒子结合，从而被吞噬细胞摄入或者激活补体级联，通过这种方式可以介导它们的破坏。
胶原凝集素都显示如下结构它们有N-末端半胱氨酸富集区，该区显示形成了链间二硫键，之后是胶原蛋白样(collagen-like)区，卷曲螺旋型α-螺旋区，最后是C-型凝集素结构域，该结构域是模式识别区，并且被称作糖识别区(CRD)。胶原凝集素的名字源自胶原蛋白和凝集素结构域的存在。由所述卷曲螺旋型α-螺旋区启动个体多肽的三聚化来形成胶原蛋白三重(triple)螺旋，从而产生胶原凝集素亚基，每个亚基由三个个体多肽组成，而N-末端区介导亚基寡聚体的形成。不同的胶原凝集素表现出独特的高级结构，通常是亚基四聚体或亚基六聚体。大量结合区的分组(grouping)使胶原凝集素以高亲合力与微生物细胞壁结合，但每个CRD对糖的内在亲合力相对较低。
在系统发生和功能方面，C-型CRD广泛出现在蛋白中。不同胶原凝集素的不同CRD使它们识别在不同微生物表面出现的一些独特的微生物表面组分。末端CRD的分布使存在结合位点的三个结构域靶表面都有约53的间距(spacing)(Sheriff等，1994；Weis & Drickamer，1994)。此“模式识别”性质可进一步造成选择性结合微生物表面。胶原凝集素的胶原蛋白区或可能是N-末端尾部被吞噬细胞上的特异性受体识别，因此它是相关蛋白酶的结合位点，这些蛋白酶在所述CRD区结合了靶之后被激活以启动补体级联。
结合甘露聚糖的凝集素(MBL)也被称作结合甘露糖的凝集素或甘露糖结合蛋白，作为先天免疫系统的重要部分，它是一种引起重视的胶原凝集素。MBL结合细菌、酵母、寄生的原生动物和病毒等在很多微生物表面发现的特异性糖结构，它也通过激活末端水解性补体组分来介导杀伤或通过促进吞噬作用来表现抗细菌活性。MBL缺陷与儿童，可能还有成人频繁感染多种微生物的易感性有关。
MBL的CRD优先识别带有平展的(equatorial)3-和4-OH基团的己糖，如甘露糖，葡萄糖，N-乙酰甘露糖胺和N-乙酰葡萄糖胺，但是不结合不符合此立体需要的糖，如半乳糖和D-岩藻糖(Weis等，1992)。所述糖选择性明显是MBL分辨自身/非自身的重要方面，并且可能被甘露糖和N-乙酰葡萄糖胺残基在微生物表面的优势(prevalence)差别来介导，一个例子是甘露糖在酵母细胞壁上的高含量，所述酵母如酿酒酵母(Saccharomyces cererisiae)和白色念珠菌(Candida albicans)。哺乳动物蛋白糖基化中的糖结构通常通过唾液酸来补充(completed)，其避免了MBL和这些寡聚糖结合，从而避免了MBL识别“自身”表面。此外，每个MBL亚基的三聚体结构可能对于识别靶是重要的。
补体是一组存在于血浆和组织液中的蛋白，它辅助机体在感染后的防御。所述补体系统通过至少三种不同途径被激活，其分别被称为经典途径，替代途径，和MBLectin途径(Janeway等，1999)。当补体因子1(C1)识别表面结合的免疫球蛋白时，所述经典途径被启动。C1复合体由两种蛋白水解酶C1r和C1s，和包括免疫球蛋白识别区的非酶部分C1q组成。C1q和MBL共享结构特征，两种分子在电子显微镜下都可见到具有花束样外型(appearance)。同样，象C1q一样，MBL被发现与两种蛋白水解酶，结合甘露聚糖的凝集素相关的蛋白酶(MASP)复合。所述三种途径均产生补体因子3(C3)转化酶，它确保C3b结合到激活表面的表面，即被靶向的微生物病原体。将C3转化为表面结合的C3b在通过吞噬作用或水解作用去除微生物病原体的过程中是关键的(Janeway等，1999)。
已经报道沙门氏菌的某些O-抗原特异性寡糖会激活C4-缺陷型豚鼠血清中的补体，然后沙门血清型C显示与MBL反应从而通过MBLlectin途径激活补体，它也被称为补体激活的MBL途径或凝集素途径。
一段时间以来猜测在产生特异性免疫反应时，先天免疫系统可能与获得性免疫系统协作，例如感染或免疫接种后的抗体反应。Fearon小组已经显示用基因技术通过融合使补体因子C3的C3d片段与蛋白抗原结合可将所述抗原的免疫原性提高1000倍或更多。此技术或其任何改良技术的实际应用仍有待时日。
补体系统对于正常免疫反应的重要性首先由Pepys提出，他发现用眼镜蛇蛇毒因子耗尽小鼠的C3后该小鼠对羊红细胞、一种胸腺依赖性抗原的抗体反应受损。在C4，C2和C3缺乏的病人和实验动物中，对于胸腺依赖性抗原的初次抗体反应减弱和IgM向IgG转换受损，这些报道支持先天免疫和获得性免疫是有联系的观点。此机制可能涉及产生C3衍生的配体，它使抗原或包含抗原的复合体与抗原呈递细胞或B细胞上的补体受体结合。因此，用特异性抗CR1和抗CR2的抗体或用水溶性受体蛋白阻断小鼠的CR1(CD35)和CR2(CD21)会减少对免疫接种的抗体反应，并且使用CR1和CR2缺陷的敲除小鼠的实验显示在对胸腺依赖性抗原的反应中需要这些受体。另外，有白细胞粘附缺陷、缺乏CD11/CD18粘附分子CR3的病人表现出抗体反应受损并且不能将IgM转换为IgG。如上所述，C3衍生的片段C3d是一种特异性CR2配体，它表现出强的剂量依赖性佐剂作用。
经典补体途径(C1，C2，C4和C3)的缺陷与被包裹的细菌的感染有关。它主要的原因可能是由于补体功能异常导致的调理和杀细菌防御机制有效性下降。然而，也可考虑对多糖抗原受损的免疫反应。未深入研究补体对胸腺非依赖性抗原的反应的影响，现有信息是矛盾的。因此，根据文献记载，C3-耗尽的小鼠和C3-缺陷的狗对胸腺非依赖性抗原的抗体反应是低的。而另一些报道发现，C3-缺陷的病人对用多糖疫苗免疫接种的反应看来是正常的。
Ficolins，象MBL一样，是包括胶原蛋白样区的凝集素。然而，与MBL不同，它们有纤维蛋白原样区，它类似纤维蛋白原的β-和γ-链。Ficolins也形成结构亚基的寡聚体，每个由三个相同的35kDa多肽组成。每个亚基由氨基末端，半胱氨酸富集区；胶原蛋白样区，其由Gly-Xaa-Yaa三联体序列的串联重复组成(其中Xaa和Yaa表示任何氨基酸)；颈区；和纤维蛋白原样区组成。Ficolin的寡聚体包含两个或更多个亚基，特别是已经观察到ficolin的四聚体形式。
一些ficolin以与MBL基本相同的方式引发了补体系统的活化。对补体系统的这种引发导致如上所述的新的丝氨酸蛋白酶(MASPs)的活化。
多种凝集素的纤维蛋白原样区与C-型凝集素包括MBL的CRD具有相同的功能，因此它们象模式识别受体一样辨别病原体和自身。
血清ficolin对GlcNAc(N-乙酰葡萄糖胺)，弹性蛋白或GalNAc(N-乙酰半乳糖胺)有共同的结合特异性。所述纤维蛋白原样区负责结合糖。在人的血清中已经鉴定了两种ficolin，L-ficolin(P35，ficolin L，ficolin 2或hucolin)和H-ficolin(Hakata抗原，ficolin 3或不耐热的b2-巨糖蛋白)，并且两种都有凝集素活性。L-ficolin识别GlcNAc，H-ficolin识别GalNAc。另一种已知是M-ficolin的ficolin(P35相关性蛋白，Ficolin 1或Ficolin A)被认为不是血清蛋白，它可见于白细胞和肺。L-ficolin和H-ficolin激活了与MASPs相关的凝集素补体途径。M-ficolin，L-ficolin和H-ficolin有钙非依赖性凝集素活性。
MASP(MBL相关性丝氨酸蛋白酶)包含MASP-1，MASP-2和MASP-3，它们是负责激活凝集素途径的蛋白水解酶。MASP的总体结构类似经典补体途径中第一种因子的两种蛋白水解组分，C1r和C1s的结构。当MBL或与MASP-1，MASP-2，MASP-3和sMAP相关的ficolin结合例如细菌、酵母、寄生的原生动物、病毒的表面的糖结构时，启动了凝集素途径。MASP-2是酶组分，它类似经典途径中的C1s，可切断补体组分C4和C2来形成C3转化酶C4bC2a，此酶对于凝集素-和经典-激活途径都是共同的。
MASP-1，MASP-2，MASP-3和sMAP由两个基因编码；sMAP是MASP-2的截断的形式，并且MASP-3是由MASP-1基因通过可变剪接产生的。MASP-1基因有MASP-1和MASP-3共有的H-链编码区，其后是对MASP-1和MASP-3特异的蛋白酶区编码区的串联重复。
MASP家族可分为两个系统发生的谱系，TCN型和AGY型谱系。TCN型谱系包括MASP-1，它有编码活性位点丝氨酸的TCN密码子(其中N表示A，G，C或T)，存在组氨酸-环二硫键和分隔(split)的多个外显子。与此相比，AGY型谱系包括MASP-2，MASP-3，C1r和C1s，其特征为编码活性位点丝氨酸的AGY密码子(其中Y表示C或T)，不存在组氨酸-环和单一外显子。
MASP-1，MASP-2，MASP-3，C1r和C1s由六个区组成两个C1r/C1s/Uegf/骨形态发生蛋白1(CUB)区；一个表皮生长因子(EGF)样区；两个补体控制蛋白(CCP)区或短共有序列重复(SCRs)，和丝氨酸蛋白酶区。组氨酸(H)，天冬氨酸(D)和丝氨酸(S)残基是在丝氨酸蛋白酶区中形成活性中心所必需的。只有MASP-1在轻链中有两个另外的半胱氨酸残基，它们形成组氨酸环二硫键(S-S)，如胰蛋白酶和胰凝乳蛋白酶中所见。在MBL和ficolin结合到病原体表面上的糖后，MASP的酶原形式在第二个CCP和蛋白酶区之间裂解，从而得到活性形式，其由两个多肽，重链和轻链组成(也称为A和B链)。
发明概述本发明涉及能够激活补体系统的融合蛋白。因此，本发明涉及一种融合蛋白，包含源自凝集素-补体途径激活蛋白(补体激活蛋白)或其功能同系物的第一多肽序列；和源自胶原凝集素或其功能同系物的第二多肽序列；其中所述补体激活蛋白不是胶原凝集素。
所述融合蛋白适用于治疗，包括产生，重建，增强和/或刺激补体系统的调理和/或杀细菌活性，即增强免疫防御识别并杀死微生物病原体的能力。因此，本发明涉及包含所述融合蛋白的药物。
本发明另一方面也涉及对有需要的个体治疗临床状况的方法，其包括给药所述个体如上限的融合蛋白。
本发明另一方面涉及治疗或预防有需要的个体的临床状况，如感染的方法，其包括对所述个体给药融合蛋白，其包含源自能够形成结构单元的寡聚体的蛋白的第一多肽序列；和源自结合甘露糖的凝集素(MBL)的第二多肽序列，其中所述第一多肽序列和所述第二肽序列不源自相同的蛋白，并且所述融合蛋白能够与甘露糖相关性丝氨酸蛋白酶(MASP)相连。所述第一多肽序列优选源自能够形成结构单元的四聚体、五聚体、和/或六聚体的蛋白。在优选实施方案中，所述第一多肽序列和所述第二多肽序列如下所述。
本发明还涉及如上述的融合蛋白在制备用于治疗有需要的个体的临床状况的药物中的用途。
本发明还涉及制备所述融合蛋白的方法，以及编码所述融合蛋白的分离的核酸序列，包含所述序列的载体以及包含所述载体的细胞。
附图简述

图1显示L-ficolin的序列。
图2显示MBL的序列。
图3显示融合蛋白的例子。
图4-8显示了实施例1所述的质粒。
图9显示实施例1所述的融合蛋白的比对。
图10显示实施例2所讨论的两个Western印迹。
定义胶原凝集素先天免疫力中结构上相关的、识别糖的一族蛋白，其包括结合甘露聚糖的凝集素(MBL)和表面活性蛋白A和D。该名字指存在有胶原蛋白样区和C-型凝集素区。
补体一组存在于血浆和组织液中、在感染后辅助机体防御的蛋白。补体参与破坏异源细胞并将吞噬细胞吸引到机体中的冲突(conflict)区域。
偶联的在两种化合物，例如免疫原性决定簇和胶原凝集素和/或胶原凝集素同系物之间，或者免疫原性决定簇和糖之间形成的连接。该连接可能是通过例如形成化学键如共价键而产生的物理连接。
CRD糖识别区，发现于胶原凝集素C-末端的C-型凝集素区。
免疫原性决定簇分子或其部分，其包含刺激宿主生物体的免疫系统产生抗原特异性分泌型体液反应和/或细胞反应的一或多个表位，或者能够在脊椎动物产生此类免疫原的DNA分子。
免疫反应对包含免疫原性决定簇的免疫原性组合物的反应。免疫反应包括在宿主中产生的对于所给予的目标组合物或疫苗的细胞和/或体液免疫反应。免疫反应通常包括特异性或非特异性针对在所给予的免疫原性组合物中存在的免疫原性决定簇的i)产生的抗体，ii)B细胞，iii)辅助性T细胞，iv)抑制性T细胞，v)细胞毒性T细胞，和vi)补体中一或多个的作用。
凝集素特异性结合糖的蛋白。
MASP甘露糖相关性丝氨酸蛋白酶。
MBL结合甘露聚糖的凝集素或结合甘露糖的凝集素。
亚基复合体＝结构单元三个融合蛋白的复合体，其类似如上讨论MBL和ficolin时的亚基复合体。

发明内容
本发明目的是提供能够激活补体系统的融合蛋白，来辅助预防或治疗疾病，特别是感染性疾病。
所述融合蛋白由源自凝集素补体途径激活蛋白(＝补体激活蛋白)或其功能同系物的第一多肽序列；和源自胶原凝集素或其功能同系物的第二多肽序列组成；其中所述补体激活蛋白不是胶原凝集素。
通过将源自凝集素补体途径激活蛋白的多肽序列和源自胶原凝集素的多肽序列组合，可以设计出对多种糖，优选细菌和病毒的糖有结合亲合力同时具有补体系统激活活性的融合蛋白。
第一多肽序列所述第一多肽序列可以源自任何凝集素补体途径激活蛋白。所述凝集素补体途径激活蛋白可以是天然出现的凝集素补体途径激活蛋白以及所述凝集素补体途径激活蛋白的变体或同系物，其中所述变体或同系物保持了凝集素补体途径激活的活性。
优选所述融合蛋白能够形成亚基复合体，每个复合体由三个如上所限定的融合蛋白组成。
所述第一多肽序列也优选能够与另一融合蛋白的第一多肽序列形成寡聚复合体，其中所述另一融合蛋白可以与第一种融合蛋白相同。因此可提供两个或更多融合蛋白或两个或更多亚基复合体的寡聚复合体，所述寡聚复合体比单体融合蛋白对细菌或病毒的糖的结合亲合力高。在优选实施方案中，所述寡聚的复合体是二聚的亚基复合体，更优选是三聚的亚基复合体，再更优选是四聚的亚基复合体。
在优选实施方案中，所述凝集素补体途径激活蛋白是如上所限的ficolin。所述ficolin可以是L-ficolin，H-ficolin或M-ficolin或其变体或同系物。在优选实施方案中，所述凝集素补体途径激活蛋白是L-ficolin。
另一实施方案中所述第一多肽序列包含所述凝集素的纤维蛋白原区，和/或凝集素的颈区，如ficolin或其变体或同系物。
当所述第一多肽序列衍生自ficolin或ficolin的变体或同系物时，优选所述第一多肽序列包含来自ficolin或ficolin的变体或同系物的胶原蛋白样区。另一实施方案中优选所述第一多肽序列包含来自ficolin或ficolin的变体或同系物的半胱氨酸富集区。甚更优选所述第一多肽序列包含来自ficolin或ficolin的变体或同系物的胶原蛋白样区和半胱氨酸富集区。
更优选所述第一多肽序列包含来自L-ficolin或L-ficolin的变体或同系物的胶原蛋白样区。另一实施方案中更优选所述第一多肽序列包含来自L-ficolin或L-ficolin的变体或同系物的半胱氨酸富集区。甚更优选所述第一多肽序列包含L-ficolin的N-末端区，其包括两个半胱氨酸氨基酸残基。
甚更优选所述第一多肽序列包含来自L-ficolin或L-ficolin的变体或同系物的胶原蛋白样区和半胱氨酸富集区。
在特别优选的实施方案中所述ficolin具有参照序列数据库和登录号如下所列的序列之一。这里描述了每一个序列的半胱氨酸富集区和胶原蛋白样区。NP_003656.ficolin 3 precursor；ficolin(collagen/fibrinogen domain-containing)3(Hakataantigen)[Homo sapiens][gi:4504331]90..299/region_name＝″pfam00147，fibrinogen_C，Fibrinogen beta and gamma chains，C-terminal globular domain″90..299/region_name＝″smart00186，FBG，Fibrinogen-related domains(FReDs)；Domainpresent at the C-termini of fibrinogen beta and gamma chains，and a variety offibrinogen-related proteins，including tenascin and Drosophila scabrous″1 mdllwilpsl wllllggpac lktqehpscp gpreleaskv vllpscpgap gspgekgapg61 pqgppgppgk mgpkgepgdp vnllrcqegp rncrellsqg atlsgwyhlc lpegralpvf121 cdmdtegggw lvfqrrqdgs vdffrswssy ragfgnqese fwlgnenlhq ltlqgnwelr181 veledfngnr tfahyatfrl lgevdhyqla lgkfsegtag dslslhsgrp fttydadhds241 snsncavivh gawwyascyr snlngryavs daaahkygid wasgrgvghp yrrvrmmlrXP_116792.similar to Ficolin 2 precursor(Collagen/fibrinogen domain-containing protein 2)(Ficolin-B)(Ficolin B)(Serum lectin P35)(EBP-37)(Hucolin)(L-Ficolin)[Homo sapiens][gi:20477458]91..168/region_name＝″pfam00147，fibrinogen_C，Fibrinogen beta and gamma chains，C-terminal globular domain″91..168/region_name＝″smart00186，FBG，Fibrinogen-related domains(FReDs)；Domainpresent at the C-termini of fibrinogen beta and gamma chains，and a variety offibrinogen-related proteins，including tenascin and Drosophila scabrous″
1 mgpallalsf lwtmaltedt cpamleyval nsepgmaskn psrrhglsll vvdqgpgarg61 vrtdqgpsga dpgslelhge cpifpseqvi lthhnnypfs tedqdndrda encavhyqga121 wwyaschlsh lngvylggar dsftnginwk sgkgnnysyk vsemkvrptO00602.Ficolin 1 precursor(Collagen/fibrinogen domain-containing protein 1)(Ficolin-A)(Ficolin A)(M-Ficolin)[gi:20455484]1..29/gene＝″FCN1″/region_name＝″Signal″/note＝″POTENTIAL.″30..326/gene＝″FCN1″/region_name＝″Mature chain″/note＝″FICOLIN 1.″55..93/gene＝″FCN1″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″133/gene＝″FCN1″/region_name＝″Conflict″/note＝″T-＞N(IN REF.1).″144..290/gene＝″FCN1″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″287/gene＝″FCN1″/region_name＝″Conflict″/note＝″N-＞S(IN REF.1).″305/gene＝″FCN1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 melsgatma rglavllvlfl hiknlpaqaa dtcpevkvvg legsdkltil rgcpglpgap61 gpkgeagvig ergerglpga pgkagpvgpk gdrgekgmrg ekgdagqsqs catgprnckd121 lldrgyflsg whtiylpdcr pltvlcdmdt dgggwtvfqr rmdgsvdfyr dwaaykqgfg181 sqlgefwlgn dnihaltaqg sselrvdlvd fegnhqfaky ksfkvadeae kyklvlgafv241 ggsagnsltg hnnnffstkd qdndvsssnc aekfqgawwy adchasnlng lylmgphesy301 anginwsaak gykysykvse mkvrpa//O75636.Ficolin 3 precursor(Collagen/fibrinogen domain-containing protein 3)(Collagen/fibrinogen domain-containing lectin 3 P35)(Hakata antigen)[gi:13124185]1..21/gene＝″FCN3″/region_name＝″Signal″/note＝″POTENTIAL.″22..299/gene＝″FCN3″/region_name＝″Mature chain″/note＝″FICOLIN 3.″48..80/gene＝″FCN3″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″50/gene＝″FCN3″/site_type＝″hydroxylation″53/gene＝″FCN3″/site_type＝″hydroxylation″59/gene＝″FCN3″/site_type＝″hydroxylation″65/gene＝″FCN3″/site_type＝″hydroxylation″68/gene＝″FCN3″/site_type＝″hydroxylation″77/gene＝″FCN3″/site_type＝″hydroxylation″119..265/gene＝″FCN3″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″189/gene＝″FCN3″/sife_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 mdllwilpsl wllllggpac lktqehpscp gpreleaskv vllpscpgap gspgekgapg61 pqgppgppgk mgpkgepgdp vnllrcqegp rncrellsqg atlsgwyhlc lpegralpvf121 cdmdtegggw lvfqrrqdgs vdffrswssy ragfgnqese fwlgnenlhq ltlqgnwelr181 veledfngnr tfahyatfrk lgevdhyqla lgkfsegtag dslslhsgrp fttydadhds241 snsncavivh gawwyascyr snlngryavs daaahkygid wasgrgvghp yrrvrmmlrXP_130120.similar to Ficolin 2 precursor(Collagen/fibrinogen domain-containing protein 2)(Ficolin-B)(Ficolin B)(Serum lectin P35)(EBP-37)(Hucolin)[Mus musculus][gi:20823464]59..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″59..89/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″60..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″60..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″60..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″60..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″60..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″61..95/region_name＝″Collagan triple helix repeat(20copies)″/note＝″Collageh″/db_xref＝″CDD:pfam01391″61..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″61..95/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″103..312/region_name＝″Fibrinogen beta and gamma chains，C-terminal globulardomain″/note＝″fibrinogen_C″/db_xref＝″CDD:pfam00147″103..312/region_name＝″Fibrinogen-related domains(FReDs)″/note＝″FBG″/db_xref＝″CDD:smart00186″1 malgsaalfv ltltvhaagt cpelkvldle gykqltilqg cpglpgaagp kgeagakgdr61 gesglpgipg kegptgpkgn qgekgirgek gdsgpsqsca tgprtckell tqghfltgwy121 tiylpdcrpl tvlcdmdtdg ggwtvfqrrl dgsvdffrdw tsykrgfgsq lgefwlgndn181 ihalttqgts elrvdlsdfe gkhdfakyss fqiqgeaeky klilgnflgg gagdsltphn241 nrlfstkdqd ndgstsscam gyhgawwysq chtsnlngly lrgphksyan gvnwkswrgy301 nysckvsemk vrliNP_056654.ficolin 2 isoform d precursor；ficolin(collagen/fibrinogen domain-containing lectin)2(hucolin)；ficolin(collagen/fibrinogen domain-containing lectin)2；hucolin[Homo sapiens][gi:8051590]39..95/region_name＝″collagen-like domain″1 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gdNP_056653.ficoli 2 isoform c precursor；ficolin(collagen/fibrinogen domain-containing lectin)2(hucolin)；ficolin(collagen/fibrinogen domain-containing lectin)2；hucolin[Homo sapiens][gi:8051588]39..95/region_name＝″collagen-like domain″102..143/region_name＝″Fibrinogen beta and gamma chains，C-terminal globulardomain″/note＝″fibrinogen_C″/db_xref＝″CDD:pfam00147″102..143/region_name＝″Fibrinogen-related domains(FReDs)″/note＝″FBG″/db_xref＝″CDD:smart00186″1 meldraygvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gprtckdlld rghflsgwht121 iylpdcrplt vlcdmdtdgg gwtvsvglgr ggqpgspggq aahlvgehtl efsillvgds181 qrNP_056652.ficolin 2 isoform b precursor；ficolin(collagen/fibrinogen domain-containing lectin)2(hucolin)；ficolin(collagen/fibrinogen domain-containing lectin)2；hucolin[Homo sapiens][gi:8051586]sig peptide1..25mat peptide26..27560..275/region_name＝″FBG domain″/note＝″fibrinogen beta/gamma homology″64..275/region_name＝″Fibrinogen-related domains(FReDs)″/note＝″FBG″/db_xref＝″CDD:smart00186″64..274/region_name＝″Fibrinogen beta and gamma chains，C-terminal globulardomain″/note＝″fibrinogen_C″/db_xref＝″CDD:pfam00147″
1 meldravgvl gaatlllsfl gmawalqaad tcpgergppg ppgkagppgp ngapgepqpc61 ltgprtckdl ldrghflsgw htiylpdcrp ltvlcdmdtd gggwtvfqrr vdgsvdfyrd121 watykqgfgs rlgefwlgnd nihaltaqgt selrvdlvdf ednyqfakyr sfkvadeaek181 ynlvlgafve gsagdsltfh nnqsfstkdq dndlntgnca vmfqgawwyk nchvsnlngr241 ylrgthgsfa nginwksgkg ynysykvsem kvrpaNP_001994.ficolin 1 precursor；ficolin(collagen/fibrinogen domain-containing)1[Homosapiens][gi:8051584]sig peptide1..27mat peptide28..32640..108/region_name＝″collagen-like domain″50..105/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″51..107/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″52..106/region_name＝″Collagen triple helixrepeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″115..326/region_name＝″FBG domain″/note＝″fibrinogen beta/gamma homology″115..326/region_name＝″Fibrinogen-related domains(FReDs)″/note＝″FBG″/db_xref＝″CDD:smart00186″115..325/region_name＝″Fibrinogen beta and gamma chains，C-terminal globulardomain″/note＝″fibrinogen_C″/db_xref＝″CDD:pfam00147″variation315/db_xref＝″dbSNP:1128428″variation316/db_xref＝″dbSNP:1128429″variation317/db_xref＝″dbSNP:1128430″1 melsgatmar glavllvlfl hiknlpaqaa dtcpevkvvg legsdkltil rgcpglpgap61 gpkgeagvig ergerglpga pgkagpvgpk gdrgekgmrg ekgdagqsqs catgprnckd121 lldrgyflsg whtiylpdcr pltvlcdmdt dgggwtvfqr rmdgsvdfyr dwaaykqgfg181 sqlgefwlgn dnihaltaqg sselrvdlvd fegnhqfaky ksfkvadsas kyklvlgafv241 ggsagnsltg hnnnffstkd qdndvsssnc aekfqgawwy adchasnlng lylmgphesy301 anginwsaak gykysykvse mkvrpaNP_004099.ficolin 2 isoform a precursor；ficolin(collagen/fibrinogen domain-containing lectin)2(hucolin)；ficolin(collagen/fibrinogen domain-containing lectin)2；hucolin[Homo sapiens][gi:4758348]sig peptide1..25mat peptide26..31339..95/region_name＝″collagen-like domain″98..313/region_name＝″FBG domain″/note＝″fibrinogen beta/gamma homology″102..313/region_name＝″Fibrinogen-related domains(FReDs)″/note＝″FBG″/db_xref＝″CDD:smart00186″102..312/region_name＝″Fibrinogen beta and gamma chains，C-terminal globulardomain″/note＝″fibrinogen_C″/db_xref＝″CDD:pfam001471 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gprtckdlld rghflsgwht121 iylpdcrplt vlcdmdtdgg gwtvfqrrvd gsvdfyrdwa tykqgfgsrl gefwlgndni181 haltaqgtse lrvdlvdfed nyqfakyrsf kvadeaekyn lvlgafvegs agdsltfhnn241 qsfstkdqdn dlntgncavm fqgawwyknc hvsnlngryl rgthgsfang inwksgkgyn301 ysykvsemkv rpaQ9WTS8.Ficolin 1 precursor(Collagen/fibrinogen domain-containing protein 1)(Ficolin-A)(Ficolin A)(M-Ficolin)[gi:13124116]1..22/gene＝″FCN1″/region_name＝″Signal″/note＝″POTENTIAL.″23..335/gene＝″FCN1″/region_name＝″Mature chain″/note＝″FICOLIN 1.″50..88/gene＝″FCN1″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″152..298/gene＝″FCN1″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″271/gene＝″FCN1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 mwwpmlwafp vllclcssqa lgqesgacpd vkivglgaqd kvaviqscps fpgppgpkge61 pgspagrger glqgspgkmg ppgskgepgt mgppgvkgek gergtasplg qkelgdalcr121 rgprsckdll trgifltgwy tiylpdcrpl tvlcdmdvdg ggwtvfqrrv dgsinfyrdw181 dsykrgfgnl gtefwlgndy lhlltangnq elrvdlrefq gqtsfakyss fqvsgeqeky241 kltlgqfleg tagdsltkhn nmafsthdqd ndtnggknca alfhgawwyh dchqsnlngr301 ylpgshesya dginwlsgrg hrysykvaem kirasQ15485.Ficolin 2 precursor(Collagen/fibrinogen domain-containing protein 2)(Ficolin-B)(Ficolin B)(Serum lectin P35)(EBP-37)(Hucolin)(L-Ficolin)[gi:13124203]1..25/gene＝″FCN2″/region_name＝″Signal″/note＝″POTENTIAL.″26..313/gene＝″FCN2″/region_name＝″Mature chain″/note＝″FICOLIN 2.″54..92/gene＝″FCN2″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″131..277/gene＝″FCN2″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″240/gene＝″FCN2″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″300/gene＝″FCN2″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gprtckdlld rghflsgwht121 iylpdcrplt vlcdmdtdgg gwtvfqrryd gsvdfyrdwa tykqgfgsrl gefwlgndni181 haltaqgtse lrvdlvdfed nyqfakyrsf kvadeaekyn lvlgafvegs agdsltfhnn241 qsfstkdqdn dlntgncavm fqgawwyknc hvsnlngryl rgthgsfang inwksgkgyn301 ysykvsemkv rpaO70497.Ficolin 2 precursor(Collagen/fibrinogen domain-containing protein 2)(Ficolin-B)(Ficolin B)(Serum lectin P35)(EBP-37)(Hucolin)[gi:13124181]＜1..15/gene＝″FCN2″/region_name＝″Signal″/note＝″POTENTIAL″16..＞306/gene＝″FCN2″/region_name＝″Mature chain″/note＝″FICOLIN 2.″41..79/gene＝″FCN2″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″130..276/gene＝″FCN2″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″299/gene＝″FCN2″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 lgsaalfvlt ltvhaagtcp elkvldlegy kqltilqgcp glpgaagpkg eagakgdrge61 sglpgipgke gptgpkgnqg ekgirgekgd sgpsqscatg prtckelltq ghfltgwyti121 ylpdcrpmtv lcdmdtdggg wtvfqrrldg svdffrdwts ykrgfgsqlg efwlgndnih181 alttqgtsel rvdlsdfegk hdfakyssfq iqgeaekykl ilgnflggga gdsltphnnr241 lfstkdqdnd gstsscamgy hgawwysqch tsnlnglylr gphksyangv nwkswrgyny301 sckvseO70165.Ficolin 1 precursor(Collagen/fibrinogen domain-containing protein 1)(Ficolin-A)(Ficolin A)(M-Ficoli)[gi:3124179]1..22/gene＝″FCN1″/region_name＝″Signal″/note＝″POTENTIAL.″23..334/gene＝″FCN1″/region_name＝″Mature chain″/note＝″FICOLIN 1.″50..88/gene＝″FCN1″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″152..298/gene＝″FCN1″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″261/gene＝″FCN1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 mqwptlwafs gllclcpsqa lgqergacpd vkvvglgaqd kvvviqscpg fpgppgpkge61 pgspagrger gfqgspgkmg pagskgepgt mgppgvkgek gdtgaapslg ekelgdtlcq121 rgprsckdll trgifltgwy tihlpdcrpl tvlcdmdvdg ggwtvfqrrv dgsidffrdw181 dsykrgfgnl gtefwlgndy lhlltangnq elrvdlqdfq gkgsyakyss fqvseeqeky241 kitlgqfleg tagdsltkhn nmsftthdqd ndansmncaa lfhgawwyhn chqsnlngry301 lsgshesyad ginwgtgqgh hysykvaemk irasP57756.Ficolin 2 precursor(Collagen/fibrinogen domain-containing protein 2)(Ficolin-B)(Ficolin B)(Serum lectin P35)(EBP-37)(Hucolin)[gi:13124114]1..22/gene＝″FCN2″/region_name＝″Signal″/note＝″POTENTIAL.″23..319/gene＝″FCN2″/region_name＝″Mature chain″/note＝″FICOLIN 2.″48..86/gene＝″FCN2″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″137..283/gene＝″FCN2″/region_name＝″Domain″/note＝″FIBRINOGEN C-TERMINAL.″306/gene＝″FCN2″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″1 mvlgsaalfv lslcvteltl haadtcpevk vldlegsnkl tilqgcpglp galgpkgeag61 akgdrgesgl pghpgkagpt gpkgdrgekg vrgekgdtgp sqscatgprt ckelltrgyf121 ltgwytiylp dcrpltvlcd mdtdgggwtv fqrridgtvd ffrdwtsykq gfgsqlgefw181 lgndnihalt tqgtnelrvd ladfdgnhdf akyssfqiqg eaekyklilg nflgggagds241 ltsqnnmlfs tkdqdndqgs sncavryhga wwysdchtsn lnglylrglh ksyangvnwk301 swkgynysyk vsemkvrliJC5980.ficolin-A precurs-mouse[gi:7513652]1..21/region_name＝″domain″/note＝″signal sequence″50..64/region_name＝″domain″/note＝″collagen-like″68..106/region_name＝″domain″/note＝″collagen-like″123..334/region_name＝″domain″/note＝″fibrinogen beta/gamma homology#label FBG″1 mqwptlwafs gllclcpsqa lgqergacpd vkvvglgaqd kvvviqscpg fpgppgpkge61 pgspagrger gfqgspgkmg pagskgepgt mgppgvkgek gdtgaapslg ekelgdtlcq121 rgprsckdll trgifltgwy tihlpdcrpl tvlcdmdvdg ggwtvfqrrv dgsidffrdw181 dsykrgfgnl gtefwlgndy lhlltangnq elrvdlqdf qgkgsyakyss fqvseeqeky241 kitlgqfleg tagdsltkhn nmsftthdqd ndansmncaa lfhgawwyhn chqsnlngry301 lsgshesyad ginwgtgqgh hysykvaemk irasS61517.ficolin-1 precurs-human[gi:2135116]1..326/note＝″36K HLA-cross-reactive plasma protein；hucolin，35K″1..22/region_name＝″domain″/note＝″signal sequence″52..108/region_name＝″region″/note＝″collagen-like″115..326/region_name＝″domain″/note＝″fibrinogen beta/gamma homology#label FBG″305/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″1 melsgatmar glavllvlfl hiknlpaqaa dtcpevkvvg legsdkltil rgcpglpgap61 gpkgeagvig ergerglpga pgkagpvgpk gdrgekgmrg ekgdagqsqs catgprnckd121 lldrgyflsg whniylpdcr pltvlcdmdt dgggwtvfqr rmdgsvdfyr dwaaykqgfg181 sqlgefwlgn dnihaltaqg sselrvdlvd fegnhqfaky ksfkvadeae kyklvlgafv241 ggsagnsltg hnnnffstkd qdndvsssnc aekfqgawwy adchasslng lylmgphesy301 anginwsaak gykysykvse mkvrpaA47172.transforming growth factor-beta 1-binding protein homolog ficolin-alpha-pig[gi:423206]112..323/region_name＝″domain″/note＝″fibrinogen beta/gamma homology#label FBG″1 mdtrgvaaam rplvllvafl ctaapaldtc pevkvvgleg sdklsilrgc pglpgaagpk61 geagasgpkg gqgppgapge pgppgpkgdr gekgepgpkg esweteqclt gprtckellt121 rghilsgwht iylpdcqplt vlcdmdtdgg gwtvfqrrsd gsvdfyrdwa aykrgfgsql181 gefwlgndhi haltaqgtne lrvdlvdfeg nhqfakyrsf qvadeaekym lvlgafvegn241 agdsltshnn slfttkdqdn dqyasncavl yqgawwynsc hvsnlngryl ggshgsfang301 vnwssgkgyn ysykvsemkf ratJC4942.ficolin-1 precursor-human[gi:2135117]1..22/region_name＝″domain″/note＝″signal sequence″45..101/region_name＝″region″/note＝″collagen-like″108..319/region_name＝″domain″/note＝″fibrinogen beta/gamma homology#label FBG″111..315/region_name＝″region″/note＝″fibrinogen-like″298/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″1 marglavllv lflhiknlpa qaadtcpevk vvglegsdkl tilrgcpglp gapgpkgeag61 vigergergl pgapgkagpv gpkgdrgekg mrgekgdagq sqscatgprn ckdlldrgyf121 lsgwhtiylp dcrpltvlcd mdtdgggwtv fqrrmdgsvd fyrdwaaykq gfgsqlgefw181 lgndnihalt aqgsselrvd lvdfegnhqf akyksfkvad eaekyklvlg afvggsagns241 ltghnnnffs tkdqdndvss sncaekfqga wwyadchasn lnglylmgph esyanginws301 aakgykysyk vsemkvrpaAAF44911.symbol＝BG:DS00929...[gi:7287873]1 mkscffvlfl wtllfevgqs sphtcpsgsp ngihqlmlpe eepfqvtqck ttardwiviq61 rrldgsvnfn qswfsykdgf gdpngeffig lqklylmtre qphelfiqlk hgpgatvyah121 fddfqvdset elyklervgk ysgtagdslr yhinkrfstf drdndesskn caaehgggww181 fhsclsr所述第一多肽优选包含所述补体激活蛋白或所述蛋白的变体或同系物的至少10个，如至少12个，例如至少15个，如至少20个，例如至少25个，如至少30个，例如至少35个，如至少40个，例如至少50个连续的氨基酸残基。此类变体或同系物优选与所述补体激活蛋白至少70％，如80％，例如90％，如95％相同。
所述融合蛋白的第一多肽序列在直接或间接与靶，如细菌或病毒结合时，优选能够活化凝集素补体途径。优选实施方案中所述第一多肽序列能够连接至少一个MASP蛋白，如选自MASP-1，MASP-2和MASP-3的MASP蛋白或其功能同系物或变体。特别是当所述第一多肽是融合蛋白的一部分时，它能够连接所述至少一个MASP蛋白。因此，所述第一多肽序列能够给所述融合蛋白提供激活补体系统的活性。
在特别优选的实施方案中，所述第一多肽序列至少包含的氨基酸残基，此氨基酸残基对应于图1所示L-ficolin序列的1-54，如图1的L-ficolin序列的1-55，如图1的L-ficolin序列的1-69，如图1的L-ficolin序列的1-77，如图1的L-ficolin序列的1-90，如图1的L-ficolin序列的1-93，如图1的L-ficolin序列的1-131，如图1的L-ficolin序列的1-207。特别是所述第一多肽序列包含选自如下的氨基酸残基图1的L-ficolin序列的1-55，图1的L-ficolin序列的1-54，图1的L-ficolin序列的1-50，或1-77。在更优选的实施方案中，所述第一多肽序列具有选自如下的氨基酸残基图1的L-ficolin序列的1-55，图1的L-ficolin序列的1-54，图1的L-ficolin序列的1-50，或1-77。在另一实施方案中，所述第一多肽序列至少包含氨基酸残基，所述氨基酸残基对应于图1所示L-ficolin序列的60-90，如图1的L-ficolin序列的55-90，如图1的L-ficolin序列的54-92。
优选选择的所述第一多肽序列和所述第二多肽序列包括基序X-X-G-X-X-G至少5次，如至少7次，优选在连续的序列中包括该基序。更优选选择所述第一多肽序列和所述第二多肽序列来使前述的基序被基序X-X-G-X-G取代一次。在所述基序中X表示任何不同于甘氨酸的氨基酸，而G表示甘氨酸。
第二多肽序列所述第二多肽序列优选能够连接一或多个糖。这可以通过至少引入目标胶原凝集素的糖识别区来实现。因此，所述第二多肽序列优选包含胶原凝集素或其同系物或变体的CRD区。
优选所述胶原凝集素选自如下MBL(结合甘露糖的凝集素)，SP-A(肺表面活性蛋白A)，SP-D(肺表面活性蛋白D)，BK(或BC，牛共凝集素)和CL-43(胶原凝集素-43)。最优选所述胶原凝集素是MBL。
在特别优选的实施方案中所述胶原凝集素具有参照序列数据库和登录号如下所列的序列之一。
胶原凝集素SEQ ID NO42Q9NPY3 Complement component C1q receptor precursor(Complement component 1，qsubcomponent，receptor 1)(C1qRp)(C1qR(p))(C1q/MBL/SPA receptor)(CD93 antigen)(CDw93)gi|21759074|sp|Q9NPY3|CD93_HUMAN[21759074]FEATURES Location/Qualifiers source 1..652/organism＝″Homosapiens″/db_xref＝″taxon:9606″gene 1..652/gene＝″C1QR1″/note＝″CD93″Protein 1..652/gene＝″C1QR1″/product＝″Complement component C1q receptor precursor″Region 1..21/gene＝″C1QR1″/region_name＝″Signal″Region 22..652/gene＝″C1QR1″/region_name＝″Mature chain″/note＝″COMPLEMENTCOMPONENT C1Q RECEPTOR.″Region 22/gene＝″C1QR1″/region_name＝″Conflict″/note＝″T-＞V(IN AA SEQUENCE).″Region 24..580/gene＝″C1QR1″/region_name＝″Domain″/note＝″EXTRACELLULAR(POTENTIAL).″Region 32..174/gene＝″C1QR1″/region_name＝″Domain″/note＝″C-TYPE LECTIN.″Region 36/gene＝″C1QR1″/region_name＝″Conflict″/note＝″C-＞T(IN AA SEQUENCE).″Region 38..39/gene＝″C1QR1″/region_name＝″Conflict″/note＝″TA-＞RI(IN AASEQUENCE).″Region 155/gene＝″C1QR1″/region_name＝″Conflict″/note＝″S-＞N(IN REF.1).″Region 186/gene＝″C1QR1″/region_name＝″Conflict″/note＝″G-＞A(IN AA SEQUENCE).″Region 260..301/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 1″Bond bond(264，275)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY″Bond bond(271，285)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(287，300)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY″Region 302..344/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 2.″Bond bond(306，317)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(311，328)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 318/gene＝″C1QR1″/region_name＝″Variant″/note＝″V-＞A./FTld＝VAR_013573.″Site 325/gene＝″C1QR1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Bond bond(330，343)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 345..384/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE3，CALCIUM-BINDING(POTENTIAL).″Bond bond(349，358)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(354，367)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(369，383)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 385..426/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 4，CALCIUM-BINDING(POTENTIAL).″Bond bond(389，400)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(396，409)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(411，425)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 427..468/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 5，CALCIUM-BINDING(POTENTIAL).″Bond bond(431，443)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(439，452)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY″Bond bond(454，467)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 492/gene＝″C1QR1″/region_name＝″Conflict″/note＝″S-＞A(IN AA SEQUENCE).″Region 496/gene＝″C1QR1″/region_name＝″Conflict″/note＝″R-＞Q(IN AA SEQUENCE).″Region 504/gene＝″C1QR1″/region_name＝″Conflict″/note＝″R-＞G(IN AA SEQUENCE).″Region 541/gene＝″C1QR1″/region_name＝″Conflict″/note＝″P-＞S(IN REF.1).″Region 581..601/gene＝″C1QR1″/region_name＝″Transmembraneregion″/note＝″POTENTIAL″Region 594..601/gene＝″C1QR1″/region_name＝″Domain″/note＝″POLY-LEU.″Region 602..652/gene＝″C1QR1″/region_name＝″Domain″/note＝″CYTOPLASMIC(POTENTIAL).″ORIGIN 1 matsmgllll llllltqpga gtgadteavv cvgtacytah sgklsaaeaq nhcnqnggnl61 atvkskeeaq hvqrvlaqll rreaaltarm skfwiglqre kgkcldpslp lkgfswvggg121 edtpysnwhk elrnsciskr cvsllldlsq pllpsrlpkw segpcgspgs pgsniegfvc181 kfsfkgmcrp lalggpgqvt yttpfqttss sleavpfasa anvacgegdk detqshyflc241 kekapdvfdw gssgplcvsp kygcnfnngg chqdcfeggd gsflcgcrpg frllddlvtc301 asrnpcsssp crggatcvlg phgknytcrc pqgyqldssq ldcvdvdecq dspcaqecvn361 tpggfrcecw vgyepggpge gacqdvdeca lgrspcaqgc tntdgsfhcs ceegyvlage421 dgtqcqdvde cvgpggplcd slcfntqgsf hcgclpgwvl apngvsctmg pvslgppsgp481 pdeedkgeke gstvpraata sptrgpegtp katpttsrps lssdapitsa plkmlapsgs541 pgvwrepsih hataasgpqe paggdssvat qnndgtdgqk lllfyilgtv vaillllala601 lgllvyrkrr akreekkekk pqnaadsysw vperaesram enqysptpgt dcSEQ ID NO43BAC05523 collectin placenta 1[Mus musculus]gi|21901969|dbj|BAC05523.1|[21901969]FEATURES Location/Qualifiers source 1..742/organism＝″Musmusculus″/db_xref＝″taxon:10090″/tissue_lib＝″Liver″Protein 1..742/product＝″collectin placenta 1″CDS1..742/gene＝″CL-P1″/coded_by＝″AB078434.1:92..2320″ORIGIN 1 mkddfaeeee vqsfgykrfg iqegtqctkc knnwalkfsi vllyilcall titvailgyk61 vvekmdnvtd gmetshqtyd nkltavesdl kklgdqagkk alstnselst frsdildlrq121 qlqeitekts knkdtleklq angdslvdrq sqlketlqnn sflittvnkt lqayngyvtn181 lqqdtsvlqg nlqsqmysqs vvimnlnnln ltqvqqrnli snlqqsvddt slaiqriknd241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql ssftgqmdni301 ttisqaneqs lkdlqdlhkd tenrtavkfs qleerfqvfe tdivniisni sytahhlrtl361 tsnlndvrtt ctdtltrhtd dltslnntlv nirldsislr mqqdmmrskl dtevanlsvv421 meemklvdsk hgqliknfti lqgppgprgp kgdrgsqgpp gptgnkgqkg ekgepgppgp481 agergtigpv gppgergskg skgsqgpKgs rgspgkpgpq gpsgdpgppg ppgkdglpgp541 qgppgtqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgame plalqneptp601 asevngcpph wknftdkcyy fslekeifed aklfcedkss hlvfinsree qqwikkhtvg661 reshwigltd seqesewkwl dgspvdyknw kagqpdnwgs ghgpgedcag liyagqwndf721 qcdeinnfic ekereavpss ilSEQ ID NO45AAM34742 46-kDa collectin precursor[Bos taurus]gi|21105685|gb|AAM34742.1|AF509589_1[21105685]sig_peptide 1..20Region 67..245/region_name＝″collagen-like region″Region 245..371/region_name＝″carbohvdrate recognition domain″CDS 1.371/gene＝″CL-46″/coded_by＝″ioin(AF509589.1:1454..1652，AF509589.1:5950..6066，AF509589.1:6402..6509，AF509589.1:6823..6930，AF509589.1:7289..7405，AF509589.1:8021..8104，AF509589.1:10318..10700)″ORIGIN 1 mlllplsvll lltqpwrslg aemkiysqkt langctlvvc rppegglpgr dgqdgregpq61 gekgdpgspg pagragrpgp agpigpkgdn gsagepgpkg dtgppgppgm pgpagregps121 gkqgsmgppg tpgpkgdtgp kggmgapgmq gspgpaglkg ergapgelga pgsagvagpa181 gaigpqgpsg argppglkgd rgdpgergak gesgladvna lkqrvtileg qlqrlqnafs241 rykkavlfpd gqavgkkifk tagavksysd aqqlcreakg qlasprsaae neavaqlvra301 knndaflsmn distegkfty ptgeslvysn wasgepnnnn agqpencvqi yregkwndvp361 csepllvice fSEQ ID NO47XP_139613 similar to collectin sub-family member 10；collectin liver 1；collectin 34[Musmusculus]gi|20903807|reg|XP_139613.1|[20903807]FEATURES Location/Qualifiers source 1..420/organism＝″Musmusculus″/strain＝″C57BL/6J″/db_xref＝″taxon:10090″/chromosome＝″15″Protein 1..420/product＝″similar to collectin sub-family member 10；collectin liver 1；collectin34″Region 152..269/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 165..269/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″Region 362..419/region_name＝″Ubiquitin-coniugating enzyme E2，catalytic domainhomologues″/note＝″UBCc″/db_xref＝″CDD:smart00212″Region 363..419/region_name＝″Ubiquitin-coniugatingenzyme″/note＝″UQ_con″/db_xref＝″CDD:pfam00179″CDS1..420/gene＝″LOC239447″/coded_by＝″XM_139613.1:1..1263″/db_xref＝″lnterimID:239447″ORIGIN 1 mngfrvllrs nlsmllllal lhfqslgldv dsrsaaevca thtispgpkg ddgergdtge61 egkdgkvgrq gpkgvkgelg dmgaqgnigk sgpigkkgdk gekgllgipg ekgkagticd121 cgryrkvvgq ldisvarlkt smkfiknvia gireteekfy yivqeeknyr eslthcrirg181 gmlampkdev vntliadyva ksgffrvfig vndleregqy vftdntplqn ysnwkeeeps241 dpsghedcve mlssgrwndt echltmyfvs slqedliedc lreqgllvqv tpanqellfg301 idtflgpmsc vyqrtgtkqk lysqcrlwdg lakkqtneta niatfckgae pnrgsrpcgq361 kqemmtlmms gnkgittfpe sdnlfkwvgt mlgaagtide dlkyklslns pvvtliihpqSEQ ID NO48XP_123211 simiar to collectin sub-family member 12[Mus musculus]gi|20876566|ref|XP_123211.1|[20876566]FEATURES Location/Qualifiers source 1..742/organism＝″Musmusculus″/strain＝″C57BL/6J″/db_xref＝″taxon:10090″/chromosome＝″18″Protein 1..742/product＝″similar to collectin sub-famliy member 12″Region 79..320/region_name＝″V-type ATPase 116kDa subunitfamily″/note＝″V_ATPase_sub_a″/db_xref＝″CDD:pfam01496″Region 92..337/region_name＝″Intermediate filamentprotein″/note＝″filament″/db_xref＝″CDD:pfam00038″Region 607..731/region_name＝″C-typelectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 624..732/region name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS1..742/gene＝″LOC225157″/coded_by＝″XM_123211.1:77..2305″/db_xref＝″InterimID:225157″ORIGIN 1 mkddfaeeee vqsfgykrfg iqegtqctkc knnwalkfsi vllyilcall titvailgyk61 vvekmdnvtd gmetshqtyd nkltavesdl kklgdqagkk alstnselst frsdildlrq121 qlqeitekts knkdtleklq angdslvdrq sqlketlqnn sflittvnkt lqayngyvtn181 lqqdtsvlqg nlqsqmysqs vvimnlnnln ltqvqqrnli snlqqsvddt slaiqriknd241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql ssftgqmdni301 ttisqaneqs lkdlqdlhkd tenrtaykfs qleerfqvfe tdivniisni sytahhlrtl361 tsnlndvrtt ctdtltrhtd dltslnntlv nirldsislr mqqdmmrskl dtevenlsvv421 meemklvdsk hgqliknfti lqgppgprgp kgdrgsqgpp gptgnkgqkg ekgepgppgp481 agergtigpv gppgergskg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkdglpgp541 qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgame plalqneptp601 asevngcpph wknftdkcyy fslekeifed aklfcedkss hlvfinsree qqwikkhtvg661 reshwigltd seqesewkwl dgspvdyknw kagqpdnwgs ghgpgedcag liyagqwndf721 qcdeinnfic ekereavpss ilSEQ ID NO49NP_571645 mannose binding-like lectin[Danio rerio]gi|18858997|ref|NP_571645.1|[18858997]sig_peptide 1..23mat_peptide 24..251/product＝″mannose binding-like lectin″Region 24..36/region_name＝″N-terminal segment″Region 33..70/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″Region 33..70/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″Region 37..101/region_name＝″collagen-like structure″Region 37..70/region_name＝″Collagen triple helix xrepeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″Region 71..74/region_name＝″break in collagen structure″Region 102..132/region_name＝″neck region″Region 133..251/region_name＝″carbohydrate recognition domain″/note＝″CRD″Region 134..247/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 146..247/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS 1..251/gene＝″mbl″/coded_by＝″NM_131570.1:68..823″/note＝″collectin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose；mannose binding-like lectin″/db_xref＝″LocusID:58091″ORIGIN 1 mallklflga lllllqlvql magaadpqsl ncpayagvpg tpghnglpgr dgrvgrdgan61 gpkgekgepg vnvqgppgka gppgpagakg ergpsglpgq dcmsdslkse lqklsdkial121 iekvvnfktf kkvgqkyyvt ddveetfdkg mqycssngga lvlprtleen allkvfvssa
181 fkrlfiritd rekegefvdt drkkltftnw gpnqpdnykg aqdcgaiads glwddvscds241 lypiceieikSEQ ID NO50NP_569057 collectin sub-family member 12，isoform l；scavenger receptor with C-type lectin；collectin placenta 1[Homo sapiens]gi|18641 360|ref|NP_569057.1|[18641360]FEATURES Location/Qualifiers source 1..742/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″18″/map＝″18pter-p11.3″Protein 1..742/product＝″collectin sub-family member 12，isoform l″/note＝″isoform l isencoded by transcript variant l；scavenger receptor with C-type lectin；collectin placenta 1″Region 79..328/region_name＝″V-type ATPase 116kDa subunitfamily″/note＝″V_ATPase_sub_a″/db_xref＝″CDD:pfam01496″Region443..589/region_name＝″collagen-like domain″Region 607..731/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 624..732/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″Region668..719/region_name＝″Beta-lactamase″/note＝″beta-lactamase″/db_xref＝″CDD:pfam00144″CDS1..742/gene＝″COLEC12″/coded_by＝″NM_130386.1:172..2400″/db_xref＝″LocusID:81035″ORIGIN 1 mkddfaeeee vqsfgykrfg iqegtqctkc knnwaIkfsi illyiIcaIl titvailgyk61 vvekmdnvtg gmetsrqtyd dkltavesdl kklgdqtgkk aistnselst frsdildlrq121 qlreitekts knkdtleklq asgdalvdrq sqlketlenn sflittvnkt lqayngyvtn181 lqqdtsvlqg nlqnqmyshn vvimnlnnln ltqvqqrnli tnlqrsvddt sqaiq riknd241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql nsftgqmeni301 ttisqaneqn lkdlqdlhkd aenrtaikfn qleerfqlfetdivniisni sytahhlrtl361 tsnlneyrtt ctdtltkhtd dltslnntla nirldsvslr mqqdlmrsrl dtevanlsvi421 meemklvdsk hgqliknfti lqgppgprgp rgdrgsqgpp gptgnkgqkg ekgepgppgp481 agergpigpa gppgerggkg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkeglpgp541 qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgavv plalqneptp601 apedngcpph wknftdkcyy fsvekeifed aklfcedkss hlvfintree qqwikkqmvg661 reshwigltd serenewkwl dgtspdyknw kagqpdnwgh ghgpgedcag liyagqwndf721 qcedvnnfic ekdretvlss alSEQ ID NO51NP_110408 collectin sub-family member 12，isoform ll；scavenger receptor with C-type lectin；collectin placenta 1[Homo sapiens]gi|18641358|ref|NP_110408.2|[18641358]FEATURES Location/Qualifiers source 1..622/organism＝″Homosepiens″/db_xref＝″taxon:9606″/chromosome＝″18″/map＝″18pter-p11.3″Protein 1..622/product＝″collectin sub-family member 12，isoform ll″/note＝″isoform ll isencoded by transcript variant ll；scavenger receptor with C-type lectin；collectin placenta 1″Region 79..328/region_name＝″V-type ATPase 116kDa subunitfamily″/note＝″V_ATPase_sub_a″/db_xref＝″CDD:pfam01496″Region 443..589/region_name＝″collagen-like domain″CDS1..622/gene＝″COLEC12″/coded_by＝″NM_030781.2:172..2040″/db_xref＝″LocusID:81035″ORIGIN 1 mkddfaeeee vqsfgykrfg iqegtqctkc knnwalkfsi illyilcall titvailgyk61 vvekmdnvtg gmetsrqtyd dkltayesdl kklgdqtgkk aistnselst frsdildlrq121 qlreitekts knkdtleklq asgdalvdrq sqlketlenn sflittvnkt lqayngyvtn181 lqqdtsvlqg nlqnqmyshn vvimnlnnln ltqvqqrnli tnlqrsvddt sqaiqriknd241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql nsftgqmeni301 ttisqaneqn lkdlqdlhkd aenrtaikfn qleerfqlfe tdivniisni sytahhlrtl361 tsnlnevrtt ctdtltkhtd dltslnntla nirldsvslr mqqdlmrsrl dtevanlsvi421 meemklvdsk hgqliknfti lqgppgprgp rgdrgsqgpp gptgnkgqkg ekgepgppgp
481 agergpigpa gppgerggkg skgsqgpkgs rgspgkpgpq gpsgdpgppg pp9keglpgp541 qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgavv plalqneptp601 apednskskp slqpggqgsa caSEQ ID NO52NP_569716 collectin sub-family member 12[Mus musculus]gi|18485494|ref|NP_569716.1|[18485494]FEATURES Location/Qualifiers source 1..742/organism＝″Musmusculus″/db_xref＝″taxon:10090″Protein 1..742/product＝″collectin sub-family member 12″Region 79..320/region_name＝″V-type ATPase 116kDa subunitfamily″/note＝″V_ATPase_sub_a″/db_xref＝″CDD:pfam01496″Region 607..731/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 629..732/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS1..742/gene＝″Colec12″/coded_by＝″NM_130449.1:77..2305″/db_xref＝″LocusID:140792″/dbxref＝″MGD:2152907”ORIGIN 1 mkddfaeeee vqsfgykrfg ihegtqctkc innwalkfsi vllyilcall titvailgyk61 vvekmdnvsd gmetshqtyd nkltavesdl kklgdqagkk alstnselst frsdildlrq121 qlqeitekts knkdtleklq angdslvdrq sqlketlqnn sflittvnkt lqayngyvtn181 lqqdtnvlqg nlqsqmysqs vvimnlnnln ltqvqqrnli snlqqsvddt slaiqriknd241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql ssftgqmdni301 ttisqaneqs lkdlqdlhkd tanrtavkfs qleerfqvfe tdivniisni sytahhlrtl361 tsnlndvwtt ctdtltrhtd dltslnntlv nirldsislr mqqdmmrskl dtevanlsvv421 meemklvdsk hgqliknfti lqgppgprgp kgdrgsqgpp gptgnkgqkg ekgepgppgp481 agergtigpv gppgergskg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkdglpgp541 qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgame plalqneptp601 asevngcpph wknftdkcyy fslekeiled aklfcedkss hlvfinsree qqwikkhtvg661 reshwigltd seqesewkwl dgspvdyknw kagqpdnwgs ghgpgedcag liyagqwndf721 qcdeinnfic ekereavpss ilSEQ ID NO53AAL61856 43kDa collectin precursor[Bos taurus]gi|18252111|gb|AAL61856.1|[18252111]FEATURES Location/Qualifiers source 1..321/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..321/product＝″43kDa collectin precursor″/name＝″CL-43；conglutinin；SP-D″Region 1..166/region_name＝″collagen-like″Region 167..193/region_name＝″alpha-helical neck″Region 195..321/region_name＝″carbohydrate-recognition domain″CDS 1..321/gene＝″CL43″/coded_by＝″join(AY071822.1:2945..3143，AY071822.1:5843..5950，AY071822.1:6273..6344，AY071822.1:6734..6850，AY071822.1:7039..7122，AY071822.1:9525..9910)″ORIGIN 1 mlplplsill lltqsqsflg eemdvysekt ltdpctlvvc appadslrgh dgrdgkegpq61 gekgdpgppg mpgpagregp sgrqgsmgpp gtpgpkgepg peggvgapgmpgspgpaglk121 gergtpgpgg aigpqgpsga mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege181 vqrlqnivtq yrkavlfpdg qavgekifkt agavksysda eqlcreakgq lasprssaen241 eavtqlvrak nkhaylsmnd iskegkftyp tggsldysnw apgepnnrak degpenclei301 ysdgnwndie creerlvice fSEQ ID NO44AAL61855 43kDa collectin precursor[Bos taurus]gi|18252109|gb|AAL61855.1|[18252109]FEATURES Location/Qualifiers source 1..321/organism＝″Bostaurus″/db_xref＝″taxon:9913″/tissue_type＝″liver″Protein 1..321/product＝″43kDa collectinprecursor″/name＝″CL-43；conglutinin；SP-D″CDS1..321/gene＝″CL43″/coded_by＝″AY071821.1:172..1137″ORIGIN 1 mlplplsil lltqsqsflg eemdvysekt ltdpctlvvc appadslrgh dgrdgkegpq61 gekgdpgppg mpgpagregp sgrqgsmgpp gtpgpkgepg peggvgapgmpgspgpaglk121 gergtpgpgg aigpqgpsga mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege181 vqrlqnivtq yrkavlfpdg qavgekifkt agavksysda eqlcreakgq lasprssaen241 eavtqlvrak nkhaylsmnd iskegkftyp tggsldysnw apgepnnrak degpenclei30 ysdgnwndie creerlvice fSEQ ID NO46BAB22581 data source:SPTR，source key:Q9Y6Z7，evidence:ISS～homolog to COLLECTIN34～putative[Mus musculus]gi|12833584|dbj|BAB22581.1|[12833584]FEATURES Location/Qualifiers source 1..272/organism＝ ″Musmusculus″/strain＝″C57BL/6J″/db_xref＝″FANTOM_DB:101O001H16″/db_xref＝″MGD:1904296″/db_xref＝″taxon:10090″/clone＝″1010001H16″/sex＝″male″/tissue_type＝″heart″/clone_lib＝″RIKEN full-length enriched mouse cDNA library″/dev_stage＝″adult″Protein 1..272/name＝″data source:SPTR，source key:Q9Y6Z7，evidence:ISS homolog toCOLLECTIN 34 putatiye″CDS1..272/coded_by＝″AK003121.1:81..899″/db_xref＝″MGD:1918943″ORIGIN 1 mmmrdlalag mlislaflsl lpsgcpqqtt edacsvqilv pglkgdagek gdkgapgrpg61 rvgptgekgd mgdkgqkgtv grhgkigpig akgekgdsgd igppgpsgep gipcecsqlr121 kaigemdnqv tqlttelkfi knavagvret eskiyllvke ekryadaqls cqarggtlsm181 pkdeaanglm asylaqagla rvfigindle kegafvysdr spmqtfnkwr sgepnnayde241 edcvemvasg gwndvachit myfmcefdke nlSEQ ID NO54NP_034905 mannose binding lectin，liver(A)[Mus musculus]gi|6754654|ref|NP_034905.1|[6754654]FEATURES Location/Qualifiers source 1..239/organism＝″Musmusculus″/db_xref＝″taxon:10090″/chromosome＝″14″/map＝″14 15.0 cM″Protein 1..239/product＝″mannose binding lectin，liver(A)″misc_feature 19..239/partial/note＝″mature protein based on homology to rat MPB-A″Region 126..236/region_name＝″C-type lectin(CTL)or carbohvdrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 135..237/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS1..239/gene＝″Mbl1″/coded_by＝″NM_010775.1:121..840″/db_xref＝″LocusID:17194″/db_xref＝″MGD:96923”ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedtlkt csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr aieeklanme aeirilkskl qltnklhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng lwndiscqas fkavcefpaSEQ ID NO55NP_034906 mannose binding lectin，serum(C)[Mus musculus]gi|6754656|ref|NP_034906.1|[6754656]sig_peptide 1..18Region 120..241/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xre＝″CDD:smart00034″Region 140..242/region_name＝″Lectin C-tvpedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS 1..244/gene＝″Mbl2″/coded_by＝″NM_010776.1:177..911″/note＝″polysaccharide-bindingcomponent of RaRF；sequence similarity to mannose-bindingproteins″/db_xref＝″LocusID:17195″/db_xref＝″MGD:96924″ORIGIN 1 msiftsflll cvvtvvyaetltegvqnscp vvtcsspgln gfpgkdgrdg akgekgepgq61 glrglqgppg kvgptgppgn pglkgavgpk gdrgdraefd tseidseiaa lrselralrn121 wvlfslsekv gkkyfvssvk kmsldrvkal csefqgsvat prnaeensai qkvakdiayl181 gitdvrvegs fedltgnrvr ytnwndgepn ntgdgedcvv ilgngkwndv pcsdsflaic241 efsdSEQ ID NO56NP_006429 collectin sub-family member 10；collectin liver 1；collectin 34[Homo sapiens]gi|5453619|ref|NP_006429.1|[5453619]FEATURES Location/Qualifiers source 1..277/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″8″/map＝″8q23-q24.1″Protein 1..277/product＝″collectin sub-family member 10″/note＝″collectin liver 1；collectin 34″Region 152..271/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 165..272/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS1..277/gene＝″COLEC10″/coded_by＝″NM_006438.2:76..909″/db_xref＝″LocusID:10584″ORIGIN 1 mngfasllrr nqfillvlfl lqiqslgldi dsrptaevca thtispgpkg ddgekgdpge61 egkhgkvgrm gpkgikgelg dmgdrgnigk tgpigkkgdk gekgllgipg ekgkagtvcd121 cgryrkfvgq ldisiarlkt smkfvknvia gireteekfy yivqeeknyr eslthcrirg181 gmlampkdea antliadyva ksgffrvfig vndleregqy mftdntplqn ysnwnegeps241 dpyghedcve mlssgrwndt echltmyfvc efikkkkSEQ ID NO57BAB72147 collectin placenta 1[Homo sapiens]gi|17026101|dbj|BAB72147.1|[17026101]FEATURES Location/Qualifiers source 1..742/organism＝″Homosapiens″/db_xref＝″taxon:9606″/sex＝″female″/tissue_lib＝″placenta″Protein 1..742/product＝″collectin placenta 1″CDS 1..742/gene＝″CL-P1″/coded_by＝″AB005145.1:71..2299″ORIGIN 1 mkddfaeeee vqsfgykrfg iqegtqtkc knnwalkfsi illyilcall titvailgyk61 vvekmdnvtg gmetsrqtyd dkltavesdl kklgdqtgkk aistnselst frsdildlrq121 qlreitekts knkdtleklq asgdalvdrq sqlketlenn sflittvnkt lqayngyvtn181 lqqdtsvlqg nlqnqmyshn vvimnlnnln ltqvqqrnli tnlqrsvddt sqaiqriknd241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql nsftgqmeni301 ttisqaneqn lkdlqdlhkd aenrtaikfn qleerfqlfe tdivniisni sytahhlrtl361 tsnlnevrtt ctdtltkhtd dltslnntla nirldsvslr mqqdlmrsrl dtevanlsvi421 meemklvdsk hgqliknfti lqgppgprgp rgdrgsqgpp gptgnkgqkg ekgepgppgp481 agergpigpa gppgerggkg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkeglpgp541 qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgavv plalqneptp601 apedngcpph wknftdkcyy fsvekeifed aklfcedkss hlvfintree qqwikkqmvg661 reshwigltd serenewkwl dgtspdyknw kagqpdnwgh ghgpgedcag liyagqwndf721 qcedvnnfic ekdretvlss alSEQ ID NO58AAF63470 mannose binding-like lectin precursor[Carassius auratus]gi|7542474|gb|AAF63470.1|AF227739_1[7542474]sig_peptide＜1..13Region 14..25/region_name＝″N-terminal segment″Region 26..93/region_name＝″collagen-like structure″Region 60..63/region_name＝″break in collagen structure″Region 94..124/region_name＝″neck region″Region 125..246/region_name＝″carbohydraterecognition domain″/note＝″CRD″CDS 1..246/gene＝″MBL″/coded_by＝″AF227739.1:＜1..742″/note＝″collectin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose″ORIGIN 1 llllqfalql ldgaepqnln cpayggvpgt pghnglpgrd grdgkdgaig pkgekgesgv61 svqgppgkag ppgtagekge rgpsgpqgsp gsesvleslk seiqqlkaki atfekvssvc121 hfrkvgqkyy itdgvvgnfd qglkscmefg gtmvsprtsa enqallklvv ssglgskkpy181 igvtdrkteg qfvdtegkql tftnwgpgqp ddykglqdcg viedtglwdd ggcgdirpim241 ceidikSEQ ID NO59AAF63469 mannose binding-like lectin precursor[Danio rerio]gi|7542472|gb|AAF63469.1|AF227738_1[7542472]sig_peptide 1..23mat_peptide 24..251/product＝″mannose binding-like lectin″Region 24..36/region_name＝″N-terminal segment″Region 37..101/region_name＝″colagen-like structure″Region 71..74/region_name＝″break in collagen structure″Region 102..132/region_name＝″neck region″Region 133..251/region_name＝″carbohydrate recognition domain″/note＝″CRD″CDS 1..251/gene＝″mbl″/coded_by＝″AF227738.1:68..823″/note＝″collectin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose″ORIGIN 1 mallklflga llllqlvlql magaadpqsl ncpayagvpg tpghnglpgr dgrvgrdgan61 gpkgekgepg vnvqgppgka gppgpagakg ergpsglpgq dcmsdslkse lqklsdkial121 iekvvnfktf kkvgqkyyvt ddveetfdkg mqycssngga lvlprtleen allkvfvssa181 fkrlfiritd rekegefvdt drkkltftnw gpnqpdnykg aqdcgaiads glwddvscds241 lypiiceiei kSEQ ID NO60AAF63468 mannose binding-like lectin precursor[Cyprinus carpio]gi|7542470|gb|AAF63468.1|AF227737_1[7542470]sig_peptide 1..23mat_peptide 24..256/product＝″mannose binding-like lectin″Region 24..35/region_name＝″N-terminal segment″Region 36..103/region_name＝″collagen-like structure″Region 70..73/region_name＝″break in collagen structure″Region 104..134/region_name＝″neck region″Region 135..256/region name＝″carbohydrate recognition domain″/note＝″CRD″CDS 1..256/gene＝″MBL″/coded_by＝″AF227737.1:67..837″/note＝″collectin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose″ORIGIN 1 malfklflgt llllqfalql ldgaepqnln cpayggvpgt pghnglpgrd grdgkdgaig61 pkgekgesgv svqgppgkag ppgpagekge rgptgsqgsp gsesvleslk seiqqlkaki121 atfekvasvg hfrqvgqkyy itdgvvgtfd qglkfckdfg gtmvfprtsa enqallklvv181 ssglsskkpy igvtdreteg rfvntegkql tftnwgpgqp ddykglqdcg viedsglwdd241 gscgdirpim ceidnkSEQ ID NO61AAK97540 surfactant protein A precursor[Gallus gallus]gi|15420996|gb|AAK97540.1|AF411083_1[15420996]sig_peptide 1..18Region 19..34/region_name＝″N-terminal segment″Region 35..43/region_name＝″putative collagen structure″Region 44..76/region_name＝″putative coil structure″Region 77..97/region_name＝″alpha-helical coil-coil structure；neck region″Region 98..222/region_name＝″carbohydrate recognition domain″Site 121..123/site_type＝″glycosylation″Site 181..183/site_type＝″glycosylation″/note＝″conserved″CDS 1..222/gere＝″SP-A″/coded_by＝″AF411083.1:61..729″ORIGIN 1 mlsysfcmia aavalltpch aqncagapel psipgvsgll glgalkryfg sllwpygeek61 lpecqwlqrq qdlstssdde lgnvllnlrq rilqlegvla ldgkitkvge kifasngkev121 nfssalesce etggtlatpm neeenkaimg ivkqynryay lgikesdtag qfkyvnnqpl181 nytswqqyep ngkgtekcve mytdgnwkdr kcnlyrltvc eySEQ ID NO62JN0450 conglutinin precursor-bovine gi|346501|pir||JN0450[346501]FEATURES Location/Qualifiers source 1..371/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..371/product＝″conglutinin precursor″/note＝″C3b-binding protein″Region 1..20/region_name＝″domain″/note＝″signal sequence″Region 21..371/region_name＝″product″/note＝″conglutinin″Region 46..214/region_name＝″region″/note＝″collagen-like″Site 63/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 63/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Region 75..371/region_name＝″product″/note＝″conglutinin-N″Site 78/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 87/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 87/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 96/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 99/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 99/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 108/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 111/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 129/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 132/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 135/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 135/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 141/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 141/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 147/site_type＝″modified″/note＝″4-hydroxyproline (Pro)″Site 153/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 159/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 159/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 162/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 162/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 171/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 195/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 198/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 198/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 210/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 210/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Region 248..369/region_name＝″domain″/note＝″C-type lectin homology#label LCH”Site 337/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″ORIGIN 1 mlllplsvll lltqpwrslg aemttfsqki lanactlvmc splesglpgh dgqdgrecph61 gekgdpgspg pagragrpgw vgpigpkgdn gfvgepgpkg dtgprgppgm pgpagregps121 gkqgsmgppg tpgpkgetgp kggvgapgiq gfpgpsglkg ekgapgetga pgragvtgps181 gaigpqgpsg argppglkgd rgdpgetgak gesglaevna lkqrvtildg hlrrfqnafs241 qykkavlfpd gqavgekifk tagavksysd aeqlcreakg qlasprssae neavtqmvra301 qeknaylsmn distegrfty ptgeilvysn wadgepnnsd egqpencvei fpdgkwndvp361 cskqllvice fSEQ ID NO63A57250 mannan-binding protein-chicken(fragment)gi|1362725|pir||A57250[1362725]FEATURES Location/Qualifiers source 1..30/organism＝″Gallus gallus″/db_xref＝″taxon:9031″Protem 1..30/product＝″mannan-binding protein″/note＝″collectin″Site 28/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″ORIGIN 1 lltcdkpeek myscpiiqcs apavnglpggdSEQ ID NO64A53570 collectin-43-bovine gi|1083017|pir||A53570[1083017]FEATURES Location/Qualifiers source 1..301/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..301/product＝″collectin-43″/note＝″lectin CL-43″Region 177..299/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 eemdvysekt ltdpctlvvc appadslrgh dgrdgkegpq gekgdpgppg mpgpagregp61 sgrqgsmgpp gtpgpkgepg peggvgapgm pgspgpaglk gergapgpgg aigpqgpsga121 mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege vqrlqnivtq yrkavlfpdg181 qavgekifkt agavksysda eqlcreakgq lasprssaen eavtqlvrak nkhaylsmnd241 iskegkftyp tggsldysnw apgepnnrak degpenclei ysdgnwndie creerlvice301 fSEQ ID NO65AAF28384 lung surfactant protein A[Sus scrofa]gi|6782434|gb|AAF28384.1|AF133668_1[6782434]FEATURES Location/Qualifiers source 1..116/organism＝″Sus scrofa″/db_xref＝″taxon:9823″Protein＜1..116/product＝″lung surfactant protein A″/function＝″involved in the innate immunesystem and lipid homeostasis within the lung″/name＝″collectin；SPA；SP-A″CDS 1..116/gene＝″SFTPA″/coded_by＝″AF133668.1:＜1..353″ORIGIN 1 avgekvfstn gqsvafdvir elcaraggri aaprspeene aiasivkkhn tyaylglveg61 ptagdffyld gtpvnytnwy pgeprgrgke kcvemytdgq wndrncqqyr laicefSEQ ID NO66AAF22145 lung surfactant protein D precursor；SPD；SP-D；CP4[Sus scrofa]gi|6760482|gb|AAF22145.2|AF132496_1[6760482]sig_peptide 1..20mat_peptide 21..378/product＝″lung surfactant protein D″CDS 1..378/gene＝″SFTPD″/coded_by＝″AF132496.2:44..1180″ORIGIN 1 mlllplsvli lltqpprslg aemktysqra vanacalvmc spmenglpgr dgrdgregpr61 gekgdpglpg avgragmpgl agpvgpkgdn gstgepgakg digpcgppgp pgipgpagke121 gpsgqqgnig ppgtpgpkge tgpkgevgal gmqgstgarg paglkgerga pgergapgsa181 gaagpagatg pqgpsgargp pglkgdrgpp gergakgesg lpgitalrgq vetlqgqvqr241 lqkafsqykk velfpngrgv gekifktggf ektfqdaqqv ctqaggqmas prseteneal301 sqlvtaqnka aflsmtdikt egnftyptge plvyanwapg epnnnggssg aencveifpn361 gkwndkacge lrlvicefSEQ ID NO67P41 317 MANNOSE-BINDING PROTEIN C PRECURSOR(MBP-C)(MANNAN-BINDINGPROTEIN)(RA-REACTIVE FACTOR P28A SUBUNIT)(RARF/P28A)gi|1346477|sp|P41317|MABC_MOUSE[1346477]FEATURES Location/Qualifiers source 1..244/organism＝″Musmusculus″/db_xref＝″taxon:10090″gene 1..244/gene＝″MBL2″Protein 1..244/gene＝″MBL2″/product＝″MANNOSE-BINDING PROTEIN C PRECURSOR″Region 1..18/gene＝″MBL2″/region_name＝″Signal″/note＝″BY SIMILARITY.″Region 3/gene＝″MBL2″/region_name＝″Conflict″/note＝″I-＞L(IN REF.1).″Region 15/gene＝″MBL2″/region_name＝″Conflict″/note＝″V-＞A(IN REF.1).″Region 19..244/gene＝″MBL2″/region_name＝″Mature chain″/note＝″MANNOSE-BINDINGPROTEIN C.″Bond bond(29)/gene＝″MBL2″/bond_type＝″disulfide″/note＝″INTERCHAIN(BYSIMILARITY).″Bond bond(34)/gene＝″MBL2″/bond_type＝″disulfide″/note＝″INTERCHAIN(BYSIMILARITY).″Region 38..96/gene＝″MBL2″/region_name＝″Domain″/note＝″COLLAGEN-LIKE(G-X-Y).″Site 43/gene＝″MBL2″/site_type＝″hydroxylation″/note＝″(POTENTIAL).″Site 58/gene＝″MBL2″/site_type＝″hydroxylation″/note＝″(POTENTIAL).″Site 69/gene＝″MBL2″/site_type＝″hydroxylation″/note＝″(POTENTIAL).″Site 78/gene＝″MBL2″/site_type＝″hydroxylation″/note＝″(POTENTIAL).″Site 81/gene＝″MBL2″/site_type＝″hydroxylation″/note＝″(POTENTIAL).″Region 149..242/gene＝″MBL2″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(151，240)/gene＝″MBL2″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(218，232)/gene＝″MBL2″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 msiftsflll cvvtvvyaet ltegvqnscp vvtcsspgln gfpgkdgrdg akgekgepgq61 glrglqgppg kvgptgppgn pglkgavgpk gdrgdraefd tseidseiaa lrselralrn121 wvlfslsekv gkkyfvssvk kmsldrvkal csefqgsvat prnaeensai qkvakdiayl181 gitdvrvegs fedltgnrvr ytnwndgepn ntgdgedcvv ilgngkwndv pcsdsflaic241 efsdSEQ ID NO68P39039 MANNOSE-BINDING PROTEIN A PRECURSOR(MBP-A)(MANNAN-BINDINGPROTEIN)(RA-REACTIVE FACTOR POLYSACCHARIDE-BINDING COMPONENT P28BPOLYPEPTIDE)(RARF P28B)gi|729972|sp|P39039|MABA_MOUSE[729972]FEATURES Location/Qualifiers source 1..239/organism＝″Musmusculus″/db_xref＝″taxon:10090″gene 1..239/gene＝″MBL1″Protein 1..239/gene＝″MBL1″/product＝″MANNOSE-BINDING PROTEIN A PRECURSOR″Region 1..17/gene＝″MBL1″/region_name＝″Signal″/note＝″BY SIMILARITY.″Region 18..239/gene＝″MBL1″/region_name＝″Mature chain″/note＝″MANNOSE-BINDINGPROTEIN A.″Region 37..8g/gene＝″MBL1″/region_name＝″Domain″/note＝″COLLAGEN-LIKE(G-X-Y).″Region 144..239/gene＝″MBL1″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(146，235)/gene＝″MBL1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(213，227)/gene＝″MBL1″/bond_type＝″disulfide″/note＝″BY SIMILARITY″ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedtlkt csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr aieeklanme aeirilkskl qltnklhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng Iwndiscqas fkavcefpaSEQ ID NO69P42916 COLLECTIN-43(CL-43)gi|1168967|sp|P42916|CL43_BOVIN[1168967]FEATURES Location/Qualifiers source 1..301/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..301/product＝″COLLECTIN-43″Region 29..142/region_name＝″Domain″/note＝″COLLAGEN-LIKE(G-X-Y).″Region 202.301/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORT FORM).″Bond bond(204，299)/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(277，291)/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 eemdvysekt ltdpctlvvC appadslrgh dgrdgkegpq gekgdpgppg mpgpagregp61 sgrqgsmgpp gtpgpkgepg peggvgapgm pgspgpaglk gergapgpgg aigpqgpsga121 mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege vqrlqnivtq yrkavlfpdg
181 qavgekifkt agavksysda eqlcreakgq lasprssaen eavtqlvrak nkhaylsmnd241 iskegkftyp tggsldysnw apgepgnrak degpenclei ysdgnwndie creerlvice301 fSEQ ID NO70CAB56155 DMBT1/8kb.2 protein[Homo sapiens]gi|5912464|emb|CAB56155.1|[5912464]sig_peptide 1..26mat_peptide 26..2412/product＝″DMBT 1/8kb.2 protein″CDS 1..2412/gene＝″DMBT1″/coded_by＝″AJ243212.1:107..7345″/note＝″Sequence is analternative splice form of the DMBT1 gene that is expressed in human adult trachea.lsoformsof DMBT1 are identical to the collectin binding protein gp-340.Full-length cDNA clonecontains 1 bp deletions in codons 100 and 1751，that were corrected by comparison with thegenomic exons″ORIGIN 1 mgistvilem cllwgqvlst ggwiprttdy aslipsevpl dttvaegspf pseltlestv61 aegspisles tlettvaegs lipsestles tvaegsdsgl alrlvngdgr cqgrveilyr121 gswgavcdds wdtndanvvc rqlgcgwams apgnawfgqg sgpialddvr csghesylws181 cphngwlshn cghgedagvi csaaqpqstl rpeswpvris ppvptegses slalrlvngg241 drcrgrvevl yrgswgtvcd dywdtndanv vcrqlgcgwa msapgnaqfg qgsgpivldd301 vrcsghesyl wscphngwlt hncghsedag vicsapqsrp tpspdtwpts hastagpess361 lalrlvnggd rcqgrvevly rgswgtvcdd swdtsdanvv crqlgcgwat sapgnarfgq421 gsgpivlddv rcsgyesylw scphngwlsh ncqhsedagv icsaahswst pspdtlptit481 lpastvgses slalrlvngg drcqgrvevl yrgswgtvcd dswdtndanv vcrqlgcgwa541 mlapgnarfg qgsgpivldd vrcsgnesyl wscphngwls hncghsedag vicsgpessl601 alrlvnggdr cqgrvevlyr gswgtvcdds wdtndanvvc rqlgcgwams apgnarfgqg661 sgpivlddvr csghesylws cpnngwlshn cghhedagvi csaaqsrstp rpdtlstitl721 ppstvgsess ltlrlvngsd rcqgrvevly rgswgtvcdd swdtndanvv crqlgcgwat781 sapgnarfgq gsgpivlddv rcsghesylw scphngwlsh ncghhedagv icsvsqsrpt841 pspdtwptsh astagpessl alrlvnggdr cqgrvevlyr gswgtvcdds wdtsdanvvc901 rqlgcgwats apgnarfgqg sgpivlddvr csgyesylws cphngwlshn cqhsedagvi961 csaahswstp spdtlptitl pastvgsess lalrlvnggd rcqgrvevly qgswgtvcdd1021 swdtndanvv crqlgcgwam sapgnarfgq gsgpivldda rcsghesylw scphngwlsh1081 ncghsedagv icsasqsrpt pspdtwptsh astagsessl airlvnggdr cqgrvevlyr1141 gswgtvcddy wdtndanvac rqlgcgwams apgnarfgqg sgpivlddvr csghesylws1201 cphngwlshn cghhedagvi csasqsqptp spdtwptsha stagsessla lrlvnggdrc1261 qgrvevlyrg swgtvcddyw dtndanvvcr qlgcgwatsa Pgnarfgqgs gpivlddvrc1321 sghesylwsc phngwlshnc ghhedagvic sasqsqptps pdtwptshas tagsesslal1381 rlvnggdrcq grvevlyrgs wgtvcddywd tndanvvcrq lgcgwatsap gnarfgqgsg1441 pivlddvrcs ghesylwscp hngwlshncg hhedagvics afqsqptpsp dtwptsrast1501 agsestlalr lvnggdrcrg rvevlyqgsw gtvcddywdt ndanvvcrql gcgwamsapg1561 naqfgqgsgp ivlddvrcsg hepylwscph ngwlshncgh hedagvicsa aqsqstprpd1621 twlttnlpal tvgsesslal rlvnggdrcr grvevlyrgs wgtvcddswd tndanvvcrq1681 lgcgwamsap gnarfgqgsg pivlgdvrcs gnesylwscp hkgwlthncg hhedagvics1741 atqinstttd wwhpttttta rpssncggfl fyasgtfssp sypayypnna kcvweievns1801 gyrinlgfsn lkleahhncs fdyveifdgs lnsslllgki cndtrqifts synrmtihfr1861 sdisfqntgf lawynsfpsd atlrlvnlns syglcagrve iyhggtwgav cddswtiqea1921 evvcrqlgcg ravsalgnay fgsgsgpitl ddvecsgtes tlwqcrnrgw fshncnhred1981 agvicsgnhl stpapflnit rpnnyscggf lsqpsgdfss Pfypgnypnn akcvwdievq2041 nnyrvtvifr dvqleggcny dyievfdgpy rsspliarvc dgargsftss snfmsirfis2101 dhsitrrgfr aeyysspsnd stnllclpnh mqasvsrsyl qslgfsasdl vistwngyye2161 crpqitpnlv iftipysgcg tfkqadndti dysnlltaav sggiikrrtd lrihvscrml2221 qntwvdtmyi andtihvann tiqveevqyg nfdvnisfyt sssflypvts rpyyvdlnqd2281 lyvqaeilhs davltlfvdt cvaspysndf tsltydlirs gcvrddtygp ysspslriar2341 frfrafhfln rfpsvylrck mvvcraydps srcyrgcvlr skrdvgsyqe kvdvvlgpiq2401 lqtpprreee prSEQ ID NO71BAA81747 collectin 34[Homo sapiens]gi|5162875|dbj|BAA81747.1|[5162875]FEATURES Location/Qualifiers source 1..277/organism＝″Homosapiens″/db_xref＝″taxon:9606″Protein 1..277/product＝″collectin 34″CDS 1..277/coded_by＝″AB002631.1:6..839″ORIGIN 1 mngfasllrr nqfillvlfl lqiqslgldi dsrptaevca thtispgpkg ddgekgdpge61 egkhgkvgrm gpkgikgelg dmgdrgnigk tgpigkkgdk gekgllgipg ekgkagtvcd121 cgryrkfvgq ldisiarlkt smkfvknvia gireteekfy yivqeeknyr eslthcrirg181 gmlampkdea antliadyva ksgffrvfig vndleregqy mftdntplqn ysnwnegeps241 dpyghedcve mlssgrwndt echltmyfvc efikkkkSEQ ID NO72AAB94071 mannan-binding lectin；collectin[Gallus gallus]gi|2736145|gb|AAB94071.1|[2736145]FEATURES Location/Qualifiers source 1..238/organism＝″Gallus gallus″/strain＝″WhiteLeghorn″/db_xref＝″taxon:9031″/tissue_type＝″liver″Protein 1..＞238/product＝″mannan-binding lectin″/name＝″c-typelectin″/note＝″mannan-binding protein；MBP；mannose-binding protein；MBL；collectin″CDS 1..238/gene＝″cMBI″/coded_by＝″AF022226.1:1..＞714″ORIGIN 1 mmatsllttd kpeekmyscp iiqcsapavn glpgrdgrdg pkgekgdpge glrglqglpg61 kagpqglkge vgpqgekgqk gergivvtdd lhrqitdlea kirvleddls rykkalslkd121 vvnigkkmfv stgkkynfek gkslcakags vlasprneae ntalkdlidp ssqayigisd181 aqtegrfmyl sggpltysnw kpgepnnhkn edcaviedsg kwndldcsns nifiicelSEQ ID NO73AAB36019 mannan-binding protein，MBP＝lectin{N-terminal}[chickens，serum，Peptide Partial，30 aa][Gallus gallus]gi|1311692|gb|AAB36019.1|[1311692]FEATURES Location/Qualifiers source 1..30/organism＝″Gallus gallus″/db_xref＝″taxon:9031″Protein 1..30/partial/product＝″mannan-binding protein″/name＝″lectin″/note＝″MBP″ORIGIN 1 lltcdkpeek myscpiiqcs apavnglpgdSEQ ID NO74AAB27504 conglutinin(N){N-terminal}[cattle，Peptide Partial，60 aa][Bos taurus]gi|386660|gb|AAB27504.1|[386660]FEATURES Location/Qualifiers source 1..60/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..60/partial/product＝″conglutinin(N)″ORIGIN 1 aemttfsqki lanactlvmc splesglpgh dgqdgrecph gekgdpgspg pagragrpgwSEQ ID NO75CAA53511 collectin-43[Bos taurus]gi|499385|emb|CAA53511.1|[499385]FEATURES Location/Qualifiers source 1..301/organism＝″Bostaurus″/db_xref＝″taxon:9913″/tissue_type＝″liver″/clone_lib＝″lambda gt 11″Protein 1..301/product＝″collectin-43″mat peptide 1..301/product＝″collectin-43″CDS 1..301/coded_by＝″X75912.1:＜1..906″/db_xref＝″SWISS-PROT:P42916″ORIGIIN 1 eemdvyxekt ltdpctlvvc appadslrgh dgrdgkegpq gekgdpgppg mpgpagregp61 sgrqgsmgpp gtpgpkgepg peggvgapgm pgspgpaglk gergapgpgg aigpqgpsga121 mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege vqrlqnivtq yrkavlfpdg181 qavgekifkt agavksysda eqlcreakgq lasprssaen eavtqlvrak nkhaylsmnd241 iskegkftyp tggsldysnw apgepgnrak degpenclei ysdgnwndie creerlvice301 fSEQ ID NO76AAA82010 mannose-binding protein C[Mus musculus]gi|773288|gb|AAA82010.1|[773288]FEATURES Location/Qualifiers source 1..244/organism＝″Musmusculus″/strain＝″BALB/c″/db_xref＝″taxon:10090″/clone＝″Lambda 14 and 52；Cos11A″/clone_lib＝″NIH/3T3 Swiss mouse embryo cell line and BALB/c pWE15 cosmidlibray″Protein 1..244/product＝″mannose-binding protein C″Site 1..59/site_type＝″signal-peptide″/note＝″signal-peptide and collagen-like region″mat_peptide<59..>98/product＝″mannose-binding protein C″/note＝″collagen-like domain″mat_peptide<98..>121/product＝″mannose-binding protein C″/note＝″linking-peptide domain″mat_peptide<121..244/product＝″carbohydrate recognition domain″CDS 1..244/gene＝″Mbl2″/coded_by＝″join(U09013.1:470..644，U09014.1:43..159，U09015.1:97..165，U09016.1:576..949)″ORIGIN 1 msiftsflll cvvtvvyaet ltegvqnscp vvtcsspgln gfpgkdgrdg akgekgepgq61 glrglqgppg kvgptgppgn pglkgavgpk gdrgdraefd tseidseiaa lrselralrn121 wvlfslsekv gkkyfvssvk kmsldnvkal csefqgsvat prnaeensai qkvakdiayl181 gitdvrvegs fedltgnrvr ytnwndgepn ntgdgedcvv ilgngkwndv pcsdsflaic241 efsdSEQ ID NO77AAA82009 mannose-binding protein A[Mus musculus]gi|773280|gb|AAA82009.1|[773280]sig_peptide 1..18mat_peptide 19..239/product＝″unnamed″mat_peptide 19..＞52/product＝″mannose-binding protein A″/note＝″collage-like region″mat_peptide<52..>91/product＝″mannose-binding protein A″/note＝″collagen-like domain″mat_peptide<91..>116/product＝″mannose-binding protein A″/note＝″linking-peptide domain″CDS 1..239/gene＝″Mbl1″/coded_by＝″join(U09007.1:275..428，U09008.1:287..403，U09009.1:166..240，U09010.1:78..451)″ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedtlkt csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr aieeklanme aeirilkskl qltnklhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng lwndiscqas fkavcefpaLung surfactant proteinSEQ ID NO78P35247 Pulmonary surfactant-associated protein D precursor(SP-D)(PSP-D)gi|464486|sp|P35247|PSPD_HUMAN[464486]FEATURES Location/Qualifiers source 1..375/organism＝″Homosapiens″/db_xref＝″taxon:9606″gene 1..375/gene＝″SFTPD″/note＝″SFTP4；PSPD″Protein 1..375/gene＝″SFTPD″/product＝″Pulmonary surfactant-associated protein Dprecursor″Region 1..20/gene＝″SFTPD″/region_name＝″Signal″/note＝″BY SIMILARITY.″Region 21..375/gene＝″SFTPD″/region_name＝″Mature chain″/note＝″PULMONARYSURFACTANT-ASSOCIATED PROTEIN D.″Region 31/gene＝″SFTPD″/region_name＝″Conflict″/note＝″M-＞T(IN REF.2).″Region 46..222/gene＝″SFTPD″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″Region 59/gene＝″SFTPD″/region_name＝″Conflict″/note＝″P-＞F(IN REF.3).″Site 78/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 87/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 90/gene＝″SFTPD″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Site 96/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 99/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Region 122/gere＝″SFTPD″/region_name＝″Conflict″/note＝″A-＞P(IN REF.2).″Site 171/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 177/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Region 180/gene＝″SFTPD″/region_name＝″Conflict″/note＝″T-＞A(IN REF.2).″Region 206/gene＝″SFTPD″/region_name＝″Conflict″/note＝″D-＞P(IN REF.3).″Region 223..252/gene＝″SFTPD″/region_name＝″Domain″/note＝″COILED COIL(POTENTIAL).″Region 227..253/gene＝″SFTPD″/region_name＝″Helical region″Region 254..256/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 257..260/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 261..262/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 263..272/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 274..283/gene＝″SFTPD″/region_name＝″Helical region″Region 279..375/gene＝″SFTPD″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(281，373)/gene＝″SFTPD″/bond_type＝″disulfide″Region 284..285/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 287..288/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 294..307/gene＝″SFTPD″/region_name＝″Helical region″Region 308/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 311..316/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 321..322/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 325/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 327..328/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 331/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 337/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 339..340/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 345..347/gene＝″SFTPD″/region_name＝″Helical region″Bond bond(351，365)/gene＝″SFTPD″/bond_type＝″disulfide″Region 351..354/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 356..357/gene＝″SFTPD″/region_name＝″Hydrogen boded turn″Region 360..363/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 365..366/gene＝″SFTPD″/region_name＝″Hydrogen bonded turn″Region 369..375/gene＝″SFTPD″/region_name＝″Beta-strand region″Region 374/gene＝″SFTPD″/region_name＝″Conflict″/note＝″E-＞EH(IN REF.3).″ORIGIN 1 mllfllsalv lltqplgyle aemktyshrt mpsactlvmc ssvesglpgr dgrdgregpr61 gekgdpglpg aagqagmpgq agpvgpkgdn gsvgepgpkg dtgpsgppgp pgvpgpagre121 galgkqgnig pqgkpgpkge agpkgevgap gmqgsagarg lagpkgergv pgergvpgnt181 gaagsagamg pqgspgargp pglkgdkgip gdkgakgesg lpdvaslrqq vealqgqvqh241 lqaafsqykk velfpngqsv gekifktagf vkpfteaqll ctqaggqlas prsaaenaal301 qqlvvaknee aflsmtdskt egkftyptge slvysnwapg epnddggsed cveiftngkw361 ndracgekrl vvcefSEQ ID NO79NP_002395 microfibrillar-associated protein 4；microfibril-associated glycoprotein 4[Homosapiens]gi|23111005|ref|NP_002395.1|[23111005]FEATURES Location/Qualifiers source 1..255/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″17″/map＝″17p11.2″Protein 1..255/product＝″microfibrillar-associated protein 4″/note＝″microfibril-associatedglycoprotein 4″Region 36..255/region_name＝″smart00186，FBG，Fibrinogen-related domains(FReDs)；Domain present at the C-termini of fibrinogen beta and gamma chains，and a variety offibrinogen-related proteins，including tenascin and Drosophila scabrous″Region 38..254/region_name＝″pfam00147，fibrinogen_C，Fibrinogen beta and gamma chains，C-terminal globular domain″CDS1..255/gene＝″MFAP4″/coded_by＝″NM_002404.1:26..793″/db_xref＝″LocusID:4239″/db_xref＝″MIM:600596″ORIGIN 1 mkallalpll lllstppcap qvsgirgdal erfclqqpld cddiyaqgyq sdgvyliyps61 gpsvpvpvfc dmtteggkwt vfqkrfngsv sffrgwndyk lgfgradgey wlglqnmhll
121 tlkqkyelrv dledfennta yakyadfsis pnavsaeedg ytlfvagfed ggagdslsyh181 sgqkfstfdr dqdlfvqhca alssgafwfr schfanlngf ylggshlsya nginwaqwkg241 fyyslkrtem kirraSEQ ID NO801KMRA Chain A，Solution Nmr Structure Of Surfactant Protein B(11-25)(Sp-B11-25)gi|22219056|pdb|1KMR|A[22219056]FEATURES Location/Qualifiers source 1..15/organism＝″Homosapiens″/db_xref＝″taxon:9606″SecStr 3..11/sec_str_type＝″helix″/note＝″helix 1″ORIGIN 1 cralikriqa mipkgSEQ ID NO81P50404 Pulmonary surfactant-associated protein D precursor(SP-D)(PSP-D)gi|1709879|sp|P50404|PSPD_MOUSE[1709879]FEATURES Location/Qualifiers source 1..374/organism＝″Musmusculus″/db_xref＝″taxon:10090″gene 1..374/gene＝″SFTPD″/note＝″SFTP4″Protein 1..374/gene＝″SFTPD″/product＝″Pulmonary surfactant-associated protein Dprecursor″Region 1..19/gene＝″SFTPD″/region_name＝″Signal″/note＝″BY SIMILARITY.″Region 20..374/gene＝″SFTPD″/region_name＝″Mature chain″/note＝″PULMONARYSURFACTANT-ASSOCATED PROTEIN D.″Region 45..221/gene＝″SFTPD″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″Site 89/gene＝″SFTPD″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Region 222..253/gene＝″SFTPD″/region_name＝″Domain″/note＝″COILED COIL(POTENTIAL).″Region 278..374/gene＝″SFTPD″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(280，372)/gene＝″SFTPD″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(350，364)/gene＝″SFTPD″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 mlpflsmlvl lvqplgnlga emkslsqrsv pntctlvmcs ptenglpgrd grdgregprg61 ekgdpglpgp mglsglqgpt gpvgpkgeng sagepgpkge rglsgppglp gipgpagkeg121 psgkqgnigp qgkpgpkgea gpkgevgapg mqgstgakgs tgpkgergap gvqgapgnag181 aagpagpagp qgapgsrgpp glkgdrgvpg drgikgesgl pdsaalrqqm ealkgklqrl241 evafshyqka alfpdgrsvg dkifrtadse kpfedaqemc kqaggqlasp rsatenaaiq301 qlitahnkaa flsmtdvgte gkftyptgep lvysnwapge pnnnggaenc veiftngqwn361 dkacgeqrlv icefSEQ ID NO82P06908 Pulmonary surfactant-associated protein A precursor(SP-A)(PSP-A)(PSAP)gi|1172693|sp|P06908|PSPA_CANFA[1172693]FEATURES Location/Qualifiers source 1..248/organism＝″Canisfamiliaris″/db_xref＝″taxon:9615″gene 1..248/gene＝″SFTPA1″/note＝″SFTPA；SFTP1″Protein 1..248/gene＝″SFTPA1″/product＝″Pulmonary surfactant-associated protein Aprecursor″Region 1..17/gene＝″SFTPA1″/region_name＝″Signal″Region 18..248/gene＝″SFTPA1″/region_name＝″Mature chain″/note＝″PULMONARYSURFACTANT-ASSOCIATED PROTEINA.″Site 20/gene＝″SFTPA1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Region 28..100/gene＝″SFTPA1″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″Region 153..248/gene＝″SFTPA1″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(155，246)/gene＝″SFTPA1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Site 207/gene＝″SFTPA1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(PROBABLE).″Bond bond(224，238)/gene＝″SFTPA1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 mwlrclalal tllmvsgien ntkdvcvgnp gipgtpgshg lpgrdgrdgv kgdpgppgpl61 gppggmpghp gpngmtgapg vagergekge pgergppglp asldeelqtt lhdlrhqilq121 tmgvlslhes llvvgrkvfs snaqsinfnd iqelcagagg qiaapmspee neavasivkk181 yntyaylglv espdsgdfqy mdgapvnytn wypgeprgrg keqcvemytd gqwnnknclq241 yrlaicefSEQ ID NO83P12842 Pulmonary surfactant-associated protein A precursor(SP-A)(PSP-A)(PSAP)gi|131413|sp|P12842|PSPA_RABIT[131413]FEATURES Location/Qualifiers source 1..247/organism＝″Oryctolaguscuniculus″/db_xref＝″taxon:9986″gene 1..247/gene＝″SFTPA1″/note＝″SFTPA；SFTP1″Protein 1..247/gene＝″SFTPA1″/product＝″Pulmonary surfactant-associated protein Aprecursor″Region 1..15/gene＝″SFTPA1″/region_name＝″Signal″/note＝″POTENTIAL.″Region 12/gene＝″SFTPA1″/region_name＝″Variant″/note＝″S-＞P.″Region 16..247/gene＝″SFTPA1″/region_name＝″Mature chain″/note＝″PULMONARYSURFACTANT-ASSOCIATED PROTEIN A.″Region 27..99/gene＝″SFTPA1″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″Region 57..60/gene＝″SFTPA1″/region_name＝″Conflict″/note＝″GPMG-＞APWA(IN REF.2).″Region 152..247/gene＝″SFTPA1″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(154，245)/gene＝″SFTPA1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Site 206/gene＝″SFTPA1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(PROBABLE).″Bond bond(223，237)/gene＝″SFTPA1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 mlllslaltl isapasdtcd tkdvcigspg ipgtpgshgl pgrdgrdgvk gdpgppgpmg61 ppggmpglpg rdgligapgv pgergdkgep gergpPglpa yldeelqatl helrhhalqs121 igvlslqgsm kavgekifst ngqsvnfdai revcaraggr iavprsleen eaiasivker181 ntyaylglae gptagdfyyl dgdpvnytnw ypgeprgqgr ekcvemytdg kwndknclqy241 rlvicefSEQ ID NO84NP_033186 surfactant associated protein D[Mus musculus]gi|6677921|ref|NP_033186.1|[6677921]sig_peptide 1..19mat_peptide 20..374/product＝″surfactant associated protein D″Region 260..373/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 271..374/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS1..374/gene＝″Sftpd″/coded_by＝″NM_009160.1:43..1167″/db_xref＝″LocusID:20390″/db_xref＝″MGD:109515″ORIGIN 1 mlpflsmlvl lvqplgnlga emkslsqrsv pntctlvmcs ptenglpgrd grdgregprg61 ekgdpglpgp mglsglqgpt gpvgpkgeng sagepgpkge rglsgppglp gipgpagkeg121 psgkqgnigp qgkpgpkgea gpkgevgapg mqgstgakgs tgpkgergap gvqgapgnag181 aagpagpagp qgapgsrgpp glkgdrgvpg drgikgesgl pdsaalrqqm ealkgklqrl241 evafshyqka alfpdgrsvg dkifrtadse kpfedaqemc kqaggqlasp rsatenaaiq
301 qlitahnkaa flsmtdvgte gkftyptgep lvysnwapge pnnnggaenc veiftngqwn361 dkacgeqrlv icefSEQ ID NO851B08C Chain C，Lung Surfactant Protein D(Sp-D)(Fragment)gi|6573321|pdb|1B08|C[6573321]FEATURES Location/Qualifiers source 1..158/organism＝″Homosapiens″/db_xref＝″taxon:9606″SecStr 13..36/sec_str_type＝″helix″/note＝″helix 7″Region 38..158/region_name＝″Domain 3″/note＝″NCBI Domains″SecStr 39..44/sec_str_type＝″sheet″/note＝″strand 21″SecStr 45..51/sec_str_type＝″sheet″/note＝″strand 22″SecStr 53..56/sec_str_tvpe＝″sheet″/note＝″strand 23″SecStr 57..67/sec_str_type＝″helix″/note＝″helix 8″Bond bond(64，156)/bond_type＝″disulfide″SecStr 77..90/sec_str_type＝″helix″/note＝″helix 9″SecStr 93..96/sec_str_type＝″sheet″/note＝″strand 24″Het join(bond(100)，bond(100)，bond(100)，bond(104)，bond(104)，bond(104)，bond(127)，bond(132)，bond(133))/heterogen＝″(CA，8)″Het join(bond(104)，bond(133)，bond(133)，bond(133))/heterogen＝″(CA，9)″SecStr 107..110/sec_str_type＝″sheet″/note＝″strand 25″SecStr 112..115/sec_str_type＝″sheet″/note＝″strand 26″Het join(bond(124)，bond(126)，bond(132)，bond(144)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145)，bond(145))/heterogen＝″(CA，7)″SecStr 133..139/sec_str_type＝″sheet″/note＝″strand 27″Bond bond(134，148)/bond_type＝″disulfide″SecStr 141..147/sec_str_type＝″sheet″/note＝″strand 28″SecStr 150..158/sec_str_type＝″sheet″/note＝″strand 29″ORIGIN 1 eaeagsvasl rqqvealqgq vqhlqaafsq ykkvelfpng qsvgekifkt agfvkpftea61 qllctqaggq lasprsaaen aalqqlvvak neaaflsmtd sktegkftyp tgeslvysnw121 apgepnddgg sedcveiftn gkwndracge krlvvcefSEQ ID NO861B08B Chain B，Lung Surfactant Protein D(Sp-D)(Fragment)gi|6573320|pdb|1B08|B[6573320]FEATURES Location/Qualifiers source 1..158/organism＝″Homosapiens″/db_xref＝″taxon:9606″SecStr 11..34/sec_str_type＝″helix″/note＝″helix 4″Region 37..158/region_name＝″Domain 2″/note＝″NCBI Domains″SecStr 39..44/sec_str_type＝″sheet″/note＝″strand 11″SecStr 45..51/sec_str_type＝″sheet″/note＝″strand 12″SecStr 53..56/sec_str_type＝″sheet″/note＝″strand 13″SecStr 57..67/sec_str_type＝″helix″/note＝″helix 5″Bond bond(64，156)/bond_type＝″disulfide″SecStr 77..90/sec_str_type＝″helix″/note＝″helix 6″SecStr 93..96/sec_str_type＝″sheet″/note＝″strand 14″SecStr 97..100/sec_str_type＝″sheet″/note＝″strand 15″Het join(bond(100)，bond(100)，bond(100)，bond(104)，bond(104)，bond(104)，bond(127)，bond(132)，bond(133))/heterogen＝″(CA，5)″Het join(bond(104)，bond(133)，bond(133)，bond(133))/heterogen＝″(CA，6)″SecStr 107..110/sec_str_type＝″sheet″/note＝″strand 16″Het join(bond(124)，bond(126)，bond(132)，bond(144)，bond(145)，bond(145))/heterogen＝″(CA，4)″SecStr 133..139/sec_str_type＝″sheet″/note＝″strand 17″Bond bond(134，148)/bond_type＝″disulfide″SecStr 141..147/sec_str_type＝″sheet″/note＝″strand 18″SecStr 150..153/sec_str_type＝″sheet″/note＝″strand 19″SecStr 154..158/sec_str_type＝″sheet″/note＝″strand 20″ORIGIN 1 eaeagsvasl rqqvealqgq vqhlqaafsq ykkvelfpng qsvgekifkt agfvkpftea61 qllctqaggq lasprsaaen aalqqlvvak neaaflsmtd sktegkftyp tgeslvysnw121 apgepnddgg sedcveiftn gkwndracge krlvvcefSEQ ID NO871B08A Chain A，Lung Surfactant Protein D(Sp-D)(Fragment)gi|6573319|pdb|1B08|A[6573319]FEATURES Location/Qualifiers source 1..158/organism＝″Homosapiens″/db_xref＝″taxon:9606″SecStr 10..36/sec_str_type＝″helix″/note＝″helix 1″Region 38..158/region_name＝″Domain 1″/note＝″NCBI Domains″SecStr 39..44/sec_str_type＝″sheet″/note＝″strand 1″SecStr 45..51/sec_str_type＝″sheet″/note＝″strand 2″SecStr 53..56/sec_str_type＝″sheet″/note＝″strand 3″SecStr 57..67/sec_str_type＝″helix″/note＝″helix 2″Bond bond(64，156)/bond_type＝″disulfide″SecStr 77..90/sec_str_type＝″helix″/note＝″helix 3″SecStr 93..96/sec_str_type＝″sheet″/note＝″strand 4″SecStr 97..100/sec_str_type＝″sheet″/note＝″strand 5″Het join(bond(100)，bond(100)，bond(100)，bond(104)，bond(104)，bond(104)，bond(127)，bond(132)，bond(133))/heterogen＝″(CA，2)″Het join(bond(104)，bond(133)，bond(133)，bond(133))/heterocen＝″(CA，3)″SecStr 107..110/sec_str_type＝″sheet″/note＝″strand 6″Het join(bond(124)，bond(126)，bond(132)，bond(144)，bond(145)，bond(145))/heterogen＝″(CA，1)″SecStr 133..139/sec_str_type＝″sheet″/note＝″strand 7″Bond bond(134，148)/bond_type＝″disulfide″SecStr 141..147/sec_str_type＝″sheet″/note＝″strand 8″SecStr 150..153/sec_str_type＝″sheet″/note＝″strard 9″SecStr 154..158/sec_str_type＝″sheet″/note＝″strand 10″ORIGIN 1 eaeagsvasl rqqvealqgq vqhlqaafsq ykkvelfpng qsvgekifkt agfvkpftea61 qllctqaggq lasprsaaen aalqqlvvak neaaflsmtd sktegkftyp tgeslvysnw121 apgepnddgg sedcveiftn gkwndracge krlvvcefSEQ ID NO88NP_06004g deleted in malignant brain tumors 1 isoform c precursor[Homo sapiens]gi|8923740|ref|NP_060049.1|[8923740]sig_peptide 1.25mat_peptide 26..2403/product＝″deleted in malignant brain tumors 1 isoform c″Region 102..202/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 105..202/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 234..334/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 237..334/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 363..463/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 366..463/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 484..584/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 487..584/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 594..692/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 595..692/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 723..823/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 726..823/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 852..952/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 855..952/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 983..1083/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 986..1083/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1112..1212/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1115..1212/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1241..1341/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1244..1341/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1370..1470/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1373..1470/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1499..1599/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1502..1599/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1630..1730/region_rame＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1633..1730/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1756..1867/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:CUB″Region 1756..1864/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 1873..1976/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1885..1976/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfan00530″Region 1998..2106/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:CUB″Region 1998..2104/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 2117..2371/region_name＝″Zona pellucida-likedomain″/note＝″zona_pellucida″/db_xref＝″CDD:pfam00100″Region 2117..2368/region_name＝″Zona pellucida(ZP)domain″/note＝″ZP″/db_xref＝″CDD:ZP″CDS 1..2403/gene＝″DMBT1″/coded_by＝″NM_017579.1:107..7318″/note＝″isoform c isencoded by transcript varia nt 3″/db_xref＝″LocusID:1755″/db_xref＝″MIM:601969″ORIGIN 1 mgistvilem cllwgqvlst ggwiprttdy aslipsevpl dttvaegspf pseltlesta61 aegspisles tlestvaegs lipsestles tvaegsdsgl alrlvngdgr cqgrveilyr121 gswgtvcdds wdtndanvvc rqlgcgwams apgnawfgqg sgpialddvr csghesylws181 cphngwlshn cghgedagvi csaaqpqstl rpeswpvris ppvptegses slalrlvngg241 drcrgrvevl yrgswgtvcd dywdtndanv vcrqlgcgwa msapgnaqfg qgsgpivldd301 vrcsghesyl wscphngwlt hncghsedag vicsaplsrp tpspdtwpts hastagpess361 lalrlvnggd rcqgrvevly rgswgtvcdd swdtsdanvv crqlgcgwat sapgnarfgq421 gsgpivlddv rcsgyesylw scphngwlsh ncqhsedagv icsdtlptit lpastvgses481 slalrlvngg drcqgrvevl yrgswgtvcd dswdtndanv vcrqlgcgwa mlapgnarfg541 qgsgpivldd vrcsgnesyl wscphngwls hncghsedag vicsgpessl alglvnggdr601 cqgrvevlyr gswgtvcdds wdtndanvvc rqlgcgwats apgnarfgqg sgpivlddvr661 csghesylws cpnngwlshn cghhedagvi csaaqsrstp rpdtlstitl ppstvgsess721 ltlrlvngsd rcqgrvevly rgswgtvcdd swdtndanvv crqlgcgwat sapgnarfgq781 gsgpivlddv rcsghesylw scphngwlsh ncghhedagv icsvsqsrpt pspdtwptsh841 astagsessl alrlvnggdr cqgrvevlyr gswgtvcdds wdtsdanvvc rrlgcgwats901 apgnarfgqg sgpivlddvr csgyesylws cphngwlshn cqhsedagvi csaahswstp961 spdtlptitl pastvgsess lalrlvnggd rcqgrvevly qgswgtvcdd swdtndanvv1021 crqlgcgwam sapgnarfgq gsgpivlddv rcsghesylw scphngwlsh ncghsedagv1081 icsasqsrpt pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr gswgtvcddy1141 wdtndanvvc rqlgcgwams apgnarfgqg sgpivlddvr csghesylws cphdgwlshn1201 cghhedagvi csasqsqptp spdtwptsha stagsassla lrlvnggdrc qgrvevlyrg1261 pwgtvcddyw dtndanvvcr qlgcgwatsa pgnarfgqgs gpivlddvrc sghesylwsc1321 phngwlshnc ghhedagvic sasqsqptps pdtwptshas tagsesslal rlvnggdrcq1381 grvevlyrgs wgtvcddywd tndanvvcrq lgcgwatsap gsarfgqgsg pialddvrcs1441 ghesylwscp hngwlshncg hhedagvics asqsqptpsp dtwptsrast agsestlalr1501 lvnggdrcrg rvevlyqgsw gtvcddywdt ndanvvcrql gcgwamsapg naqfgqgsgp1561 ivlddvrcsg hesylwscph ngwlshncgh hedagvicsa aqsqstprpd twlttnlpal1621 tvgsesslal rlvnggdrcr grvevlyrgs wgtvcddswd tndanvvcrq lgcgwamsap1681 gnarfgqgsg pivlddvrcs gnesylwscp hkgwlthncg hhedagvics atqinstttd1741 wwhpttttta rpssncggfl fyasgtfssp sypayypnna kcvweievns gyrinlgfsn1801 lkleahhncs fdyveifdgs lnsslllgki cndtrqifts synrmtihfr sdisfqntgf1861 lawynsfpsd atlrlvnlns syglcagrye iyhggtwgtv cddswtiqea evvcrqlgcg1921 ravsalgnay fgsgsgpitl ddvecsgtes tlwqcrnrgw fshncnhred agvicsgnhl1981 stpapflnit rpntdyscgg flsqpsgdfs spfypgnypn nakcvwdiev qnnyrvtvif2041 rdvqleggcn ydyievfdgp yrsspliarv cdgargsfts ssnfmsirfi sdhsitrrgf2101 iaeyysspsn dstnilclpn hmqasvsrsy lqslgfsasd lvistwngyy ecrpqitpnl2161 viftipysgc gtfkqadndt idysnfltaa vsggiikrrt dlrihvscrm lqntwvdtmy2221 iandtihvan ntiqveevqy gnfdvnisfy tsssflypvt srpyyvdlnq dlyvqaeilh2281 sdavltlfvd tcvaspysnd ftsltydlir sgcvrddtyg pysspslria rfrfrafhfl2341 nrfpsvylrc kmvvcraydp ssrcyrgcvl rskrdvgsyq ekvdvvlgpi qlqtpprree2401 eprSEQ ID NO89NP_015568 deleted in malignant brain tumors 1 isoform b precursor[Homo sapiens]gi|6633801|ref|NP_015568.1|[6633801]sig_peptide 1..25mat_peptide 26..2413/product＝″deleted in malignant brain tumors 1 isoform b″Region 102..202/region_name＝″Scavenger receptorCvs-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 105..202/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 234..334/region_name＝″Scavenger receptorCys-rich/note＝″SR″/db_xref＝″CDD:SR″Region 237..334/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 363..463/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 366..463/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 494..594/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 497..594/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 602..702/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 605..702/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 733..833/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 736..833/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 862..962/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 865..962/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 993..1093/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 996..1093/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1122..1222/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1125..1222/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1251..1351/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1254..1351/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1380..1480/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1383..1480/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1509..1609/region_name＝″Scavenger receptorCys-ric″/note＝″SR″/db_xref＝″CDD:SR″Region 1512..1609/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1640..1740/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1643..1740/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1766..1877/region_name＝″Domain first found in C1r，C1s，uEGF，ard bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:CUB″Region 1766..1874/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 1883..1986/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1895..1986/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 2008..2116/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:CUB″Region 2008..2114/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 2127..2381/region_name＝″Zona pellucida-likedomain″/note＝″zona_pellucida″/db_xref＝″CDD:pfam00100″Region 2127..2378/region_name＝″Zona pellucida(ZP)domain″/note＝″ZP″/db_xref＝″CDD:ZP″CDS1..2413/gene＝″DMBT1″/coded_by＝″NM_007329.1:107..7348″/db_xref＝″LocusID:1755″/db_xref＝″MIM:601969″ORIGIN 1 mgistvilem cllwgqvlst ggwiprttdy aslipsevpl dqtvaegspf psestlesta61 aegspisles tlestvaegs lipsestles tvaegsdsgl alrlvngdgr cqgrveliyr121 gswgtvcdds wdtndanvvc rqlgcgwams apgnawtgqg sgpialddvr csghesylws181 cphngwlshn cghgedagvi csaaqpqstl rpeswpvris ppvptegses slalrlvngg241 drcrgrvevl yrgswgtvcd dywdtndanv vcrqlgcgwa msapgnaqfg qgsgpivldd301 vrcsghesyl wscphngwlt hncghsedag vicsapqsrp tpspdtwpts hastagpess361 lalrlvnggd rcqgrvevly rgswgtvcdd swdtsdanvv crqlgcgwat sapgnarfgq421 gsgpivlddv rcsgyesylw scphngwlsh ncqhsedagv icsaahswst pspdtlptit481 lpastvgses slalrlvngg drcqgrvevl yrgswgtvcd dswdtndanv vcrqlgcgwa541 mlapgnarfg qgsgpivldd vrcsgnesyl wscphngwls hncghsedag vicsgpessl601 alrlvnggdr cqgrvevlyr gswgtvcdds wdtndanvvc rqlgcgwams apgnarfgqg661 sgpivlddvr csghesylws cpnngwlshn cghhedagvi csaaqsrstp rpdtlstitl721 ppstvgsess ltlrlvngsd rcqgrvevly rgswgtvcdd swdtndarvv crqlgcgwam781 sapgnarfgq gsgpivlddv rcsghesylw scphngwlsh ncghhedagv icsvsqsrpt841 pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr gswgtvcdds wdtsdanvvc901 rqlgcgwats apgnarfgqg sgpivlddvr csgyesylws cphngwlshn cqhsedagvi961 csaahswstp spdtlptitl pastvgsess lalrlvnggd rcqgrvevly qgswgtvcdd1021 swdtndanvv crqpgcgwam sapgnarfgq gsgpivlddv rcsghesypw scphngwlsh1081 ncghsedagv icsasqsrpt pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr1141 gswgtvcddy wdtndanvvc rqlgcgwams apgnarfgqg sgpivlddvr csghesylws1201 cphngwlshn cghhedagvi csasqsqptp spdtwptsha stagsessla lrlvnggdrc1261 qgrvevlyrg swgtvcddyw dtndanvvcr qlgcgwatsa pgnarfgqgs gpivlddvrc1321 sghesylwsc phngwlshnc ghhedagvic sasqsqptps pdtwptshas tagsesslal1381 rlvnggdrcq grvevlyrgs wgtvcddywd tndanvvcrq lgcgwatsap gnarfgqgsg1441 pivlddvrcs ghesylwscp hngwlshncg hhedagvics asqsqptpsp dtwptsrast1501 agsestlalr lvnggdrcrg rvevlyqgsw gtvcddywdt ndanvvcrql gcgwamsapg1561 naqfgqgsgp ivlddvrcsg hesylwscph ngwlshncgh hedagvicsa aqsqstprpd1621 twlttnlpal tvgsesslal rlvnggdrcr grvevlyrgs wgtvcddswd tndanvvcrq1681 lgcgwamsap gnarfgqgsg pivlddvrcs gnesylwscp hkgwlthncg hhedagvics1741 atqinstttd wwhpttttta rpssncggfl fyasgtfssp sypayypnna kcvweievns1801 gyrinlgfsn lkleahhncs fdyveifdgs lnsslllgki cndtrqifts synrmtihfr1861 sdisfqntgf lawynsfpsd atlrlvnlns syglcagrve iyhggtwgtv cddswtiqea1921 evvcrqlgcg ravsalgnay fgsgsgpitl ddvecsgtes tlwqcrnrgw fshncnhred1981 agvicsgnhl stpapflnit rpntdyscgg flsqpsgdfs spfypgnypn nakcvwdiev2041 qnnyrvtvif rdvqleggcn ydyievfdgp yrsspliarv cdgargsfts ssnfmsirfi2101 sdhsitrrgf raeyysspsn dstnllclpn hmqasvsrsy lqslgfsasd lvistwngyy2161 ecrpqitpnl viftipysgc gtfkqadndt idysnfltaa vsggiikrrt dlrihvscrm2221 lqntwvdtmy iandtihvan ntiqveevqy gnfdvnisfy tsssflypvt srpyyvdlnq2281 dlyvqaeilh sdavltlfvd tcvaspysnd ftsltydlir sgcvrddtyg pysspslria2341 rfrfrafhfl nrfpsvylrc kmvvcraydp ssrcyrgcvl rskrdvgsyq ekvdvvlgpi2401 qlqtpprree eprSEQ ID NO90NP_004397 deleted in malignant brain tumors 1 isoform a precursor[Homo sapiens]gi|4758170|ref|NP_004397.1|[4758170]sig_peptide 1..25mat_peptide 26..1785/product＝″deleted in malignant brain tumors 1 isoform a″Region 102..202/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 105..202/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 234..334/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 237..334/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 363..463/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 366..463/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 494..594/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 497..594/region_name＝″Scavenger receptor cysteie-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 623..723/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 626..723/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 752..852/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 755..852/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 881..981/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 884..981/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1012..1112/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1015..1112/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1138..1249/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:CUB″Region 1138..1246/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 1255..1358/region_name＝″Scavenger receptorCys-rich″/note＝″SR″/db_xref＝″CDD:SR″Region 1267..1358/region_name＝″Scavenger receptor cysteine-richdomain″/note＝″SRCR″/db_xref＝″CDD:pfam00530″Region 1380..1488/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:CUB″Region 1380..1486/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 1499..1751/region_name＝″Zona pellucida-likedomain″/note＝″zona_pellucida″/db_xref＝″CDD:pfam00100″Region 1499..1750/region_name＝″Zona pellucida(ZP)domain″/note＝″ZP″/db_xref＝″CDD:ZP″CDS1..1785/gene＝″DMBT1″/coded_by＝″NM_004406.1:107..5464″/db_xref＝″LocusID:1755″/db_xre＝″MIM:601969″ORIGIN 1 mgistvilem cllwgqvlst ggwiprttdy aslipsevpl dqtvaegspf psestlesta61 aegspisles tlestvaegs lipsestles tvaegsdsgl alrlvngdgr cqgrveilyr121 gswgtvcdds wdtndanvvc rqlgcgwams apgnawfgqg sgpialddvr csghesylws181 cphngwlshn cghgedagvi csaaqpqstl rpeswpvris ppvptegses slalrlvngg241 drcrgrvevl yrgswgtvcd dywdtndanv vcrqlgcgwa msapgnaqfg qgsgpivldd301 vrcsghesyl wscphngwlt hncghsedag vicsapqsrp tpspdtwpts hastagpess361 lalrlvnggd rcqgrvevly rgswgtvcdd swdtsdanvv crqlgcgwat sapgnarfgq421 gsgpivlddv rcsgyesylw scphngwlsh ncqhsedagv icsaahswst pspdtlptit481 lpastvgses slalrlvngg drcqgrvevl yqgswgtvcd dswdtndanv vcrqpgcgwa541 msapgnartg qgsgpivldd vrcsghesyp wscphngwls hncghsedag vicsasqsrp601 tpspdtwpts hastagsess lalrlvnggd rcqgrvevly rgswgtvcdd ywdtndanvv661 crqlgcgwam sapgnarfgq gsgpivlddv rcsghesylw scphngwlsh ncghhedagv721 icsasqsqpt pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr gswgtvcddy
781 wdtndanvvc rqlgcgwats apgnarfgqg sgpivlddvr csghesylws cphngwlshn841 cghhedagvi csasqsqptp spdtwptsra stagsestla lrlvnggdrc rgrvevlyqg901 swgtvcddyw dtndanvvcr qlgcgwamsa pgnaqfgqgs gpivlddvrc sghesylwsc961 phngwlshnc ghhedagvic saaqsqstpr pdtwlttnlp altvgsessl alrlvnggdr1021 crgrvevlyr gswgtvcdds wdtndanvvc rqlgcgwams apgnarfgqg sgpivlddvr1081 csgnesylws cphkgwlthn cghhedagvi csatqinstt tdwwhptttt tarpssncgg1141 flfyasgtfs spsypayypn nakcvweiev nsgyrinlgf snlkleahhn csfdyveifd1201 gslnsslllg kicndtrqif tssynrmtih frsdisfqnt gflawynsfp sdatlrlvnl1261 nssyglcagr veiyhggtwg tvcddswtiq eaevvcrqlg cgravsalgn ayfgsgsgpi1321 tlddvecsgt estlwqcrnr gwfshncnhr edagvicsgn hlstpapfln itrpntdysc1381 ggflsqpsgd fsspfypgny pnnakcvwdi evqnnyrvtv ifrdvqlegg cnydyievfd1441 gpyrssplia rvcdgargsf tsssnfmsir fisdhsitrr gfraeyyssp sndstnllcl1501 pnhmqasvsr sylqslgfsa sdlvistwng yyecrpqitp nlviftipys gcgtfkqadn1561 dtidysnflt aavsggiikr rtdlrihvsc rmlqntwvdt myiandtihv anntiqveev1621 qygnfdvnis fytsssflyp vtsrpyyvdl nqdlyvqaei lhsdavltlf vdtcvaspys1681 ndftsltydl irsgcvrddt ygpysspslr iarfrfrafh flnrfpsvyl rckmvvcray1741 dpssrcyrgc vlrskrdvgs yqekvdvvlg piqlqtpprr eeeprSEQ ID NO91LNBOC1 pulmonary surfactant protein C-bovine gil7428752|pir||LNBOC1[7428752]FEATURES Location/Qualifiers source 1..34/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..34/product＝″pulmonary surfactant protein C″/note＝″pulmonary surfactant proteinPSP-6″Site 4/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″Site 5/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″ORIGIN 1 lipccpvnik rllivvvvvv llvvvivgal lmglSEQ ID NO92LNDGC1 pulmonary surfactant protein C-dog gi|7428750|pir||LNDGC1[7428750]FEATURES Location/Qualifiers source 1..35/organism＝″Canisfamiliaris″/db_xref＝″taxon:9615″Protein 1..35/product＝″pulmonary surfactant protein C″Site 5/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″ORIGIN 1 lgipcfpssl krlliivvvi vlvvvvivga llmgl//SEQ ID NO93JN0450 conglutinin precursor-bovine gi|346501|pir|JN0450[346501]FEATURES Location/Qualifiers source 1..371/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..371/product＝″conglutinin precursor″/note＝″C3b-binding protein″Region 1..20/region_name＝″domain″/note＝″signal sequence″Region 21..371/region_name＝″product″/note＝″conglutinin″Region 46..214/region_name＝″region″/note＝″collagen-like″Site 63/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 63/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Region 75..371/region_name＝″product″/note＝″conglutinin-N″Site 78/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 87/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 87/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 96/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 99/site_type＝″binding″/note＝″carbohyd rate(Lys)(covalent)″Site 99/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 108/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 111/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 129/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 132/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 135/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 135/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 141/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 141/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 147/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 153/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 159/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 159/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 162/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 162/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 171/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 195/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 198/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 198/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 210/site_type＝″binding″/note＝″carbohydrate(Lys)(covalent)″Site 210/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Region 248..369/region_name＝″domain″/note＝″C-type lectin homology#label LCH″Site 337/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″ORIGIN 1 mlllplsvll lltqpwrslg aemttfsqki lanactlvmc splesglpgh dgqdgrecph61 gekgdpgspg pagragrpgw vgpigpkgdn gfvgepgpkg dtgprgppgm pgpagregps121 gkqgsmgppg tpgpkgetgp kggvgapgiq gfpgpsglkg ekgapgetga pgrag/tgps181 gaigpqgpsg argppglkgd rgdpgetgak gesglaevna lkqrvtildg hlrrfqnafs241 qykkavlfpd gqavgekifk tagavksysd aeqlcreakg qlasprssae neavtqmvra301 qeknaylsmn distegrfty ptgeilvysn wadgepnnsd egqpencvei fpdgkwndvp361 cskqllvice fSEQ ID NO94A45225 pulmonary surfactant protein D precursor-human gi|346375|pir||A45225[346375]FEATURES Location/Qualifiers source 1.375/organism＝″Homosapiens″/db_xref＝″taxon:9606″Protein 1..375/product＝″pulmonary surfactant protein D precursor″/note＝″SP-D″Region 1..20/region_name＝″domain″/note＝″signal sequence″Region 21..375/region_name＝″product″/note＝″pulmonary surfactant protein D″Region 21..45/region_name＝″domain″/note＝″non-collagenous″Region 46..222/region_name＝″domain″/note＝″collagenous″Site 90/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″Region 223..375/region_name＝″domain″/note＝″non-collagenous″Region 254..373/region_name＝″domain″/note＝″C-type lectin homology#label LCH″Bond bond(281，373)/bond_type＝″disulfide″Bond bond(351，365)/bond_type＝″disulfide″ORIGIN 1 mllfllsalv lltqplgyle aemktyshrt mpsactlvmc ssvesglpgr dgrdgregpr61 gekgdpglpg aagqagmpgq agpvgpkgdn gsvgepgpkg dtgpsgppgp pgvpgpagre121 galgkqgnig pqgkpgpkge agpkgevgap gmqgsagarg lagpkgergv pgergvpgnt181 gaagsagamg pqgspgargp pglkgdkgip gdkgakgesg lpdvaslrqq vealqgqvqh241 lqaafsqykk velfpngqsv gekifktagf vkpfteaqll ctqaggqlas p rsaaenaal301 qqlwaknea aflsmtdskt egkftyptge slvysnwapg epnddggsed cveiftngkw361 ndracgekrl vvcefSEQ ID NO95LNHUC pulmonary surfactant protein C precursor，long splice form-humangi|71983|pir||LNHUC[71983]FEATURES Location/Qualifiers source 1..197/organism＝″Homosapiens″/db_xref＝″taxon:9606″Protein 1..197/product＝″pulmonary surfactant protein C precursor，long spliceform″/note＝″3.7kDa surfactant polypeptide；pulmonary surfactant protein SP5；pulmonarysurfactant proteolipid SP-C；pulmonary surfactant proteolipid SPL(pVal)″Region 1..197/region_name＝″product″/note＝″pulmonary surfactant protein C precursor，shortsplice form″Region 1..145/region_name＝″product″/note＝″pulmonary surfactant protein C precursor，shortsplice form″Region 1..23/region_name＝″domain″/note＝″propeptide″Region 24..58/region_name＝″product″/note＝″pulmonary surfactant protein C″Site 28/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″Site 29/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″Region 152..197/region_name＝″product″/note＝″pulmonary surfactant protein C precursor，short splice form″ORIGIN 1 mdvgskevlm esppdysaap rgrfgipccp vhlkrllivv vvvvlivvvivgallmglhm61 sqkhtemvle msigapeaqq rlalsehlvt tatfsigstg lvvydyqqll iaykpapgtc121 cyimkiapes ipslealnrk vhnfqmecsl qakpavptsk lgqaegrdag sapsggdpaf181 lgmavntlcg evplyyiSEQ ID NO96LNDGPS pulmonary surfactant protein A precursor-dog gi|71970|pir||LNDGPS[71970]FEATURES Location/Qualifiers source 1..248/organism＝″Canisfamiliaris″/db_xref＝″taxon:9615″Protein 1..248/product＝″pulmonary surfactant protein A precursor″/note＝″pulmonarysurfactant 32K apoprotein；pulmonary surfactant-associated protein PSP-A″Region 1..17/region_name＝″domain″/note＝″signal sequence″Region 18..248/region_name＝″product″/note＝″pulmonary surfactant protein A″Site 20/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″Region 28..102/region_name＝″region″/note＝″collagen-like″Site 30/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Region 127..246/region_name＝″domain″/note＝″C-type lectin homology#label LCH″Site 207/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″ORIGIN 1 mwlrclalal tllmvsgien ntkdvcvgnp gipgtpgshg lpgrdgrdgv kgdpgppgpl61 gppggmpghp gpngmtgapg vagergekge pgergppglp asldeelqttlhdlrhqilq121 tmgvlslhes llvvgrkvfs sgaqsinfnd iqelcagagg qiaapmspee neavasivkk181 yntyaylglv espdsgdfqy mdgapvnytn wypgeprgrg keqcvemytd gqwnnknclq241 yrlaicefSEQ ID NO97LNHUPS pulmonary surfactant protein A precursor(genomic clone)-humangi|71967|pir||LNHUPS[71967]FEATURES Location/Qua|ifiers source 1..248/organism＝″Homosapiens″/db_xref＝″taxon:9606″Protein 1..248/product＝″pulmonary surfactant protein A precursor(genomicclone)″/note＝″alveolar proteinosis protein；pulmonary surfactant 32K apoprotein；pulmonarysurfactant-associated protein(PSP-A)″Region 1..20/region_name＝″domain″/note＝″signal sequence″Region 21..248/region_name＝″product″/note＝″pulmonary surfactant protein A″Bond bond(26)/bond_type＝″disulfide″/note＝″interchain″Region 28..100/region_name＝″domain″/note＝″collagenous″Site 30/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 33/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 36/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 42/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 51/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 57/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 63/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 76/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 79/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 82/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 88/site_type＝″modified″/note＝″5-hydroxylysine(Lys)″Site 91/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 97/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Region 127..246/region_name＝″domain″/note＝″C-type lectin homology#label LCH″Bond bond(155，246)/bond_type＝″disulfide″Site 207/site_type＝″binding″/note＝″carbohydrate(Asn)(covalent)″Bond bond(224，238)/bond_type＝″disulfide″ORIGIN 1 mwlcplalnl ilmaasgavc evkdvcvgsp gipgtpgshg lpgrhgrdgl kgdlgppgpm61 gppgempcpp gndglpgapg ipgecgekge p9ergppglp ahldeelqat lhdfrhqilq121 trgalslqgs imtvgekvfs sngqsitfda iqeacaragg riavprnpee neaiasfvkk181 yntyayvglt egpspgdfry sdgtpvnytn wyrgepagrg keqcvemytd gqwndrncly241 srlticefSEQ ID NO98A53570 collectin-43-bovine gi|083017|pir||A53570[1083017]FEATURES Location/Qualifiers source 1..301/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..301/product＝″collectin-43″/note＝″lectin CL-43″Region 177..299/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 eemdvysekt ltdpctlvvc appadslrgh dgrdgkegpq gekgdpgppg mpgpagregp61 sgrqgsmgpp gtpgpkgepg peggvgapgm pgspgpaglk gergapgpgg aigpqgpsga121 mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege vqrlqnivtq yrkavlfpdg181 qavgekifkt agavksysda eqlcreakgq lasprssaen eavtqlvrak nkhaylsmnd241 iskegkftyp tggsldysnw apgepnnrak degpenclei ysdgnwndie creerlvice 301 fSEQ ID NO99S33603 surfactant protein D-bovine gi|423283|pir||S33603[423283FEATURES Location/Qualifiers source 1..369/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..369/product＝″surfactant protein D″Region 248..367/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 mlllplsvll lltqpwrslg aemkiysqkt manactlvmc sppedglpgr dgrdgregpr61 gekgdpgspg pagragmpgp agpiglkgdn gsagepgpkg dtgppgppgm pgpagregps121 gkqgsmgppg tpgpkgdtgp kggvgapgiq gspgpaglkg ergapgdpga pgragapgpr181 gaigpqgpsg argppglkgd rgtpgergak gesglaevna lrqrvgileg qlqrlqnafs241 qykkamlfpn grsvgekifk tvgsektfqd aqqictqagg qlpsprsgae nealtqlata301 qnkaaflsms dtrkegtfiy ptgeplvysn wapqepnndg gsencveifp ngkwndkvcg 361eqrlvicefSEQ ID NO100AAF28384 lung surfactant protein A[Sus scrofa]gi|6782434|gb|AAF28384.1|AF133668_1[6782434]FEATURES Location/Qualifiers source 1..116/organism＝″Sus scrofa″/db_xref＝″taxon:9823″Protein＜1..116/product＝″lung surfactant protein A″/function＝″involved in the innate immunesystem and lipid homeostasis within the lung″/name＝″collectin；SPA；SP-A″CDS 1..116/gene＝″SFTPA″/coded_by＝″AF133668.1:＜1..353″ORIGIN 1 avgekvfstn gqsvafdvir elcaraggri aaprspeene aiasivkkhn tyaylglveg61 ptagdffyld gtpvnytnwy pgeprgrgke kcvemytdgq wndrncqqyr laicefSEQ ID NO101AAF22145 lung surfactant protein D precursor；SPD；SP-D；CP4[Sus scrofa]gi|6760482|gb|AAF22145.2|AF132496_1[6760482]sig_peptide 1..20mat_peptide 21..378/product＝″lung surfactant protein D″CDS 1..378/gene＝″SFTPD″/coded_by＝″AF132496.2:44..1180″ORIGIN 1 mlllplsvli lltqpprslg aemktysqravanacalvmc spmenglpgr dgrdgregpr61 gekgdpglpg avgragmpgl agpvgpkgdn gstgepgakg digpcgppgp pgipgpagke121 gpsgqqgnig ppgtpgpkge tgpkgevgal gmqgstgarg paglkgerga pgergapgsa
181 gaagpagatg pqgpsgargp pglkgdrgpp gergakgesg lpgitalrqq vetlqgqvqr241 lqkafsqykk velfpngrgv gekifktggf ektfqdaqqv ctqaggqmas prseteneal301 sqlvtaqnka aflsmtdikt egnftyptge plvyanwapg epnnnggssg aencveifpn 361gkwndkacge lrlvicefSEQ ID NO102P15783 PULMONARY SURFACTANT-ASSOCIATED PROTEIN C(SP-C)(PULMONARYSURFACTANT-ASSOCIATED PROTEOLIPID SPL(VAL))gi|131422|sp|P15783|PSPC_BOVIN[131422]FEATURES Location/Qualifers source 1..34/organism＝″Bos taaurus″/db_xref＝″taxon:9913″gene 1..34/gene＝″SFTPC″/note＝″SFTP2″Protein 1..34/gene＝″SFTPC″/product＝″PULMONARY SURFACTANT-ASSOCIATEDPROTEIN C″Site 4/gene＝″SFTPC″/site_type＝″lipid-binding″/note＝″PALMITATE(BY SIMILARITY).″Site 5/gene＝″SFTPC″/site_type＝″lipid-binding″/note＝″PALMITATE(BY SIMILARITY).″Region 21/gene＝″SFTPC″/region_name＝″Conflict″/note＝″L-＞V(IN REF.2).″Region 26/gene＝″SFTPC″/region_name＝″Conflict″/note＝″I-＞V(IN REF.2)″Region 28..34/gene＝″SFTPC″/region_name＝″Conflict″/note＝″GALLMGL-＞IGAMLAM(INREF.2).″ORIGIN 1 liccpvnik rllivvvvvv llvvvivgal lmglSEQ ID NO103P35246 PULMONARY SURFACTANT-ASSOCIATED PROTEIN D PRECURSOR(SP-D)(PSP-D)gi|464485|sp|P35246|PSPD_BOVIN[464485]FEATURES Location/Qualifiers source 1..369/organism＝″ Bos taurus″/db_xref＝″taxon:9913″gene 1..369/gene＝″SFTPD″/note＝″SFTP4″Protein 1..369/gene＝″SFTPD″/product＝″PULMONARY SURFACTANT-ASSOCIATEDPROTEIN D PRECURSOR″Region 1..20/gene＝″SFTPD″/region_name＝″Signal″/note＝″BY SIMILARITY.″Region 21..369/gene＝″SFTPD″/region_name＝″Mature chain″/note＝″PULMONARYSURFACTANT-ASSOCIATED PROTEIN D.″Region 46..216/gene＝″SFTPD″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″Site 78/gene＝″SFTPD″/site_type＝″hydtoxylation″/note＝″(BY SIMILARITY).″Site 87/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 90/gene＝″SFTPD″/site_type＝″glycosylation″/note＝″POTENTIAL.″Site 96/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 99/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 165/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Site 171/gene＝″SFTPD″/site_type＝″hydroxylation″/note＝″(BY SIMILARITY).″Region 217..248/gene＝″SFTPD″/region_name＝″Domain″/note＝″COILED COIL(POTENTIAL).″Region 273..369/gene＝″SFTPD″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(275，367)/gene＝″SFTPD″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(345，359)/gene＝″SFTPD″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 mlllplsvll lltqpwrslg aemkiysqkt manactlvmc sppedglpgr dgrdgregpr61 gekgdpgspg pagragmpgp agpiglkgdn gsagepgpkg dtgppgppgm pgpagregps121 gkqgsmgppg tpgpkgdtgp kggvgapgiq gspgpaglkg ergapgepga pgragapgpa181 gaigpqgpsg argppglkgd rgtpgergak gesglaevna lrqrvgileg qlqrlqnafs241 qykkamlfpn grsvgekifk tvgsektfqd aqqictqagg qlpsprsgae nealtqlata301 qnkaaflsms dtrkegtfiy ptgeplvysn wapqepnndg gsencveifp ngkwndkvcg361 eqrlvicefSEQ ID NO104P42916 COLLECTIN-43(CL-43)gi|1168967|sp|P42916|CL43_BOVIN[1168967]FEATURES Location/Qualifiers source 1..301/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..301/product＝″COLLECTIN-43″Region 29..142/region_name＝″Domain″/note＝″COLLAGEN-LIKE(G-X-Y).″Region 202..301/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORT FORM).″Bond bond(204，299)/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(277，291)/bond_type＝″disulfide″/note＝″BY SIMILARITY.″ORIGIN 1 eemdvysekt ltdpctlvvc appadslrgh dgrdgkegpq gekgdpgppg mpgpagregp61 sgrqgsmgpp gtpgpkgepg peggvgapgm pgspgpaglk gergapgpgg aigpqgpsga121 mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege vqrlqnivtq yrkavlfpdg181 qavgekifkt agavksysda eqlcreakgq lasprssaen eavtqlvrak nkhaylsmnd241 iskegkftyp tggsldysnw apgepgnrak degpenclei ysdgnwndie creerlvice301 fSEQ ID NO105CAB56155 DMBT1/8kb.2 protein[Homo sapiens]gi|5912464|emb|CAB56155.1|[5912464]sig_peptide 1..26mat_peptide 26..2412/product＝″DMBT1/8kb.2 protein″CDS 1..2412/gene＝″DMBT1″/coded_by＝″AJ243212.1:107..7345″/note＝″Sequence is analternative splice form of the DMBT1 gene that is expressed in human adult trachea.lsoformsof DMBT1 are identical to the collectin binding protein gp-340.Full-length cDNA clonecontains 1 bp deletions in codons 100 and 1751，that were corrected by compa rison with thegenomic exons″ORIGIN 1 mgistvilem cllwgqvlst ggwiprtdy aslipsevpl dttvaegspf pseltlestv61 aegspisles tlettvaegs lipsestles tvaegsdsgl alrlvngdgr cqgrveilyr121 gswgavcdds wdtndanvvc rqlgcgwams apgnawfgqg sgpialddvr csghesylws181 cphngwlshn cghgedagvi csaaqpqstl rpeswpvris ppvptegses slalrlvngg241 drcrgrvevl yrgswgtvcd dywdtndanv vcrqlgcgwa msapgnaqfg qgsgpivldd301 vrcsghesyl wscphngwlt hncghsedag vicsapqsrp tpspdtwpts hastagpess361 lalrlvnggd rcqgrvevly rgswgtvcdd swdtsdanvv crqlgcgwat sapgnarfgq421 gsgpivlddv rcsgyesylw scphngwlsh ncqhsedagv icsaahswst pspdtlptit481 lpastvgses slalrlvngg drcqgrvevl yrgswgtvcd dswdtndanv vcrqlgcgwa541 mlapgnarfg qgsgpivldd vrcsgnesyl wscphngwls hncghsedag vicsgpessl601 alrlvnggdr cqgrvevlyr gswgtvcdds wdtndanvvc rqlgcgwams apgnarfgqg661 sgpivlddvr csghesylws cpnngwlshn cghhedagvi csaaqsrstp rpdtlstitl721 ppstvgsess ltlrlvngsd rcqgrvevly rgswgtvcdd swdtndanvv crqlgcgwat781 sapgnarfgq gsgpivlddv rcsghesylw scphngwlsh ncghhedagv icsvsqsrpt841 pspdtwptsh astagpessl alrlvnggdr cqgrvevlyr gswgtvcdds wdtsdanvvc901 rqlgcgwats apgnarfgqg sgpivlddvr csgyesylws cphngwlshn cqhsedagvi961 csaahswstp spdtlptitl pastvgsess lalrlvnggd rcqgrvevly qgswgtvcdd1021 swdtndanvv crqlgcgwam sapgnarfgq gsgpivldda rcsghesylw scphngwlsh1081 ncghsedagv icsasqsrpt pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr1141 gswgtvcddy wdtndanvac rqlgcgwams apgnarfgqg sgpivlddvr csghesylws1201 cphngwlshn cghhedagvi csasqsqptp spdtwptsha stagsessla lrlvnggdrc1261 qgrvevlyrg swgtvcddyw dtndanvvcr qlgcgwatsa pgnarfgqgs gpivlddvrc1321 sghesylwsc phngwlshnc ghhedagvic sasqsqptps pdtwptshas tagsesslal1381 rlvnggdrcq grvevlyrgs wgtvcddywd tndanvvcrq lgcgwatsap gnarfgqgsg1441 pivlddvrcs ghesylwscp hngwlshncg hhedagvics afqsqptpsp dtwptsrast1501 agsestlalr lvnggdrcrg rvevlyqgsw gtvcddywdt ndanvvcrql gcgwamsapg1561 naqfgqgsgp ivlddvrcsg hepylwscph ngwlshncgh hedagvicsa aqsqstprpd1621 twlttnlpal tvgsesslal rlvnggdrcr grvevlyrgs wgtvcddswd tndanvvcrq1681 lgcgwamsap gnarfgqgsg pivlgdvrcs gnesylwscp hkgwlthncg hhedagvics1741 atqinstttd wwhpttttta rpssncggfl fyasgtfssp sypayypnna kcvweievns1801 gyrinlgfsn lkleahhncs fdyveifdgs lnsslllgki cndtrqifts synrmtihfr1861 sdisfqntgf lawynsfpsd atlrlvnlns syglcagrve iyhggtwgav cddswtiqea1921 evvcrqlgcg ravsalgnay fgsgsgpitl ddvecsgtes tlwqcrnrgw fshncnhred1981 agvicsgnhl stpapflnit rpnnyscggf lsqpsgdfss pfypgnypnn akcvwdievq2041 nnyrvtvifr dvqleggcny dyievfdgpy rsspliarvc dgargsftss snfmsirfis2101 dhsitrrgfr aeyysspsnd stnllclpnh mqasvsrsyl qslgfsasdl vistwngyye2161 crpqitpnlv iftipysgcg tfkqadndti dysnlltaav sggiikrrtd lrihvscrml2221 qntwvdtmyi andtihvann tiqveevqyg nfdvnisfyt sssflypvts rpyyvdlnqd2281 lyvqaeilhs davltlfvdt cvaspysndf tsltydlirs gcvrddtygp ysspslriar2341 frfrafhfln rfpsvylrck mvvcraydps srcyrgcvlr skrdvgsyqe kvdvvlgpiq2401 lqtpprreee prSEQ ID NO106AAD49696 gp-340 variant protein[Homo sapiens]gi|5733598|gb|AAD49696.1|AF159456_1[5733598]FEATURES Location/Qualifiers source 1..2413/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q25.3-26.1″Protein 1..2413/product＝″gp-340 variant protein″/name＝″scavenger receptor cysteine-richprotein SRCR″/note＝″putative receptor for SP-D″CDS 1..2413/gene＝″DMBT1″/coded_by＝″AF159456.1:107..7348″ORIGIN 1 mgistvilem cllwgqvlst ggwiprttdy aslipsevpl dqtvaegspf psestlesta61 aegspisles tlestvaegs lipsestles tvaegsdsgl alrlvngdgr cqgrveilyr121 gswgtvcdds wdtndanvvc rqlgcgwams apgnawtgqg sgpialddvr csghesylws181 cphngwlshn cghgedagvi csaaqpqstl rpeswpvris ppvptegses slalrlvngg241 drcrgrvevl yrgswgtvcd dywdtndanv vcrqlgcgwa msapgnaqfg qgsgpivldd301 vrcsghesyl wscphngwlt hncghsedag vicsapqsrp tpspdtwpts hastagpess361 lalrlvnggd rcqgrvevly rgswgtvcdd swdtsdanvv crqlgcgwat sapgnarfgq421 gsgpivlddv rcsgyesylw scphngwlsh ncqhsedagv icsaahswst pspdtlptit481 lpastvgses slalrlvngg drcqgrvevl yrgswgtvcd dswdtndanv vcrqlgcgwa541 mlapgnarfg qgsgpivldd vrcsgnesyl wscphngwls hncghsedag vicsgpessl601 alrlvnggdr cqgrvevlyr gswgtvcdds wdtndanvvc rqlgcgwams apgnarfgqg661 sgpivlddvr csghesylws cpnngwlshn cghhedagvi csaaqsrstp rpdtlstitl721 ppstvgsess ltlrlvngsd rcqgrvevly rgswgtvcdd swdtndanvv crqlgcgwam781 sapgnarfgq gsgpivlddv rcsghesylw scphngwlsh ncghhedagv icsvsqsrpt841 pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr gswgtvcdds wdtsdanvvc901 rqlgcgwats apgnarfgqg sgpivlddvr csgyesylws cphngwlshn cqhsedagvi961 csaahswstp spdtlptitl pastvgsess lalrlvnggd rcqgrvevly qgswgtvcdd1021 swdtndanvv crqpgcgwam sapgnarfgq gsgpivlddv rcsghesypw scphngwlsh1081 ncghsedagv icsasqsrpt pspdtwptsh astagsessl alrlvnggdr cqgrvevlyr1141 gswgtvcddy wdtndanvvc rqlgcgwams apgnarfgqg sgpivlddvr csghesylws1201 cphngwlshn cghhedagvi csasqsqptp spdtwptsha stagsessla lrlvnggdrc1261 qgrvevlyrg swgtvcddyw dtndanvvcr qlgcgwatsa pgnarfgqgs gpivlddvrc1321 sghesylwsc phngwlshnc ghhedagvic sasqsqptps pdtwptshas tagsesslal1381 rlvnggdrcq grvevlyrgs wgtvcddywd tndanvvcrq lgcgwatsap gnarfgqgsg1441 pivlddvrcs ghesylwscp hngwlshncg hhedagvics asqsqptpsp dtwptsrast1501 agsestlalr lvnggdrcrg rvevlyqgsw gtvcddywdt ndanvvcrql gcgwamsapg1561 naqfgqgsgp ivlddvrcsg hesylwscph ngwlshncgh hedagvicsa aqsqstprpd1621 twlttnlpal tvgsesslal rlvnggdrcr grvevlyrgs wgtvcddswd tndanvvcrq1681 lgcgwamsap gnarfgqgsg pivlddvrcs gnesylwscp hkgwlthncg hhedagvics1741 atqinstttd wwhpttttta rpssncggfl fyasgtfssp sypayypnna kcvweievns1801 gyrinlgfsn lkleahhncs fdyveifdgs lnsslllgki cndtrqifts synrmtihfr1861 sdisfqntgf lawynsfpsd atlrlvnlns syglcagrve iyhggtwgtv cddswtiqea1921 evvcrqlgcg ravsalgnay fgsgsgpitl ddvecsgtes tlwqcrnrgw fshncnhred1981 agvicsgnhl stpapflnit rpntdyscgg flsqpsgdfs spfypgnypn nakcvwdiev2041 qnnyrvtvif rdvqleggcn ydyievfdgp yrsspliarv cdgargsfts ssnfmsirfi2101 sdhsitrrgf raeyysspsn dstnllclpn hmqasvsrsy lqslgfsasd lvistwngyy2161 ecrpqitpnl viftipysgc gtfkqadndt idysnfltaa vsggiikrrt dlrihvscrm2221 lqntwvdtmy iandtihvan ntiqveevqy gnfdvnisfy tsssflypvt srpyyvdlnq2281 dlyvqaeilh sdavltlfvd tcvaspysnd ftsltydlir sgcvrddtyg pysspslria2341 rfrfrafhfl nrfpsvylrc kmvvcraydp ssrcyrgcvl rskrdvgsyq ekvdvvlgpi2401 qlqtpprree eprSEQ ID NO107AAD31380 surfactant protein D precursor[Mus musculus]gi|4877556|gb|AAD31380.1|AF047742_1[4877556]sig_peptide 1..19mat_peptide 20..374/product＝″surfactant protein D″CDS 1..374/gene＝″Sftp4″/coded_by＝″join(AF047741.1:5705..5900，AF047742.1:312.428，AF047742.1:669..785，AF047742.1:1112..1228，AF047742.1:1977..2093，AF047742.1:3162..3245，AF047742.1:5010..5386)″ORIGIN 1 mlpflsmlvl lvqplgnlga emkslsqrsv pntctlvmcs ptenglpgrd grdgregprg61 ekgdpglpgp mglsglqgpt gpvgpkgeng sagepgpkge rglsgppglp gipgpagkeg121 psgkqgnigp qgkpgpkgea gpkgevgapg mqgstgAkgs tgpkgergap gvqgapgnag181 aagpagpagp qgapgsrgpp glkgdrgvpg drgikgesgl pdsaalrqqm ealkgklqrl241 evafshyqka alfpdgrsvg dkifrtadse kpfedaqemc kqaggqlasp rsatenaaiq301 qlitahnkaa flsmtdvgte gkftyptgep lvysnwapge pnnnggaenc veiftngqwn361 dkacgeqrlv icefSEQ ID NO108B61249 pulmonary surfactant protein C-dog gi|539712|pir||B61249[539712]FEATURES Location/Qualifiers source 1..35/organism＝″Canisfamiliaris″/db_xref＝″taxon:9615″Protein 1..35/product＝″pulmonary surfactant protein C″ORIGIN 1 lgipcfpssl krlliivvvi vlvvvvivga llmglSEQ ID NO109S00609 pulmonary surfactant protein C-bovine gi|89749|pir||S00609[89749]FEATURES Location/Qualifiers source 1..34/organism＝″Bos taurus″/db_xref＝″taxon:9913″Protein 1..34/product＝″pulmonary surfactant protein C″/note＝″pulmonary surfactant proteinPSP-6″Site 4/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″Site 5/site_type＝″binding″/note＝″palmitate(Cys)(covalent)″ORIGIN 1 lipccpvnik rllivvvvvv llvvvivgal lmglSEQ ID NO110A43628 pulmonary surfactant protein A-human(fragments)gi|280854|pir||A43628[280854]FEATURES Location/Qualifiers source 1..35/organism＝″Homosapiens″/db_xref＝″taxon:9606″Protein 1..35/product＝″pulmonary surfactant protein A″ORIGIN 1 gqsitfdagk eqcvemytdg qwndrnclyl ticefSEQ ID NO111AAB48076 Surfactant protein B(SP-B)[Oryctolagus cuniculus]gi|1850933|gb|AAB48076.1|[1850933]FEATURES Location/Qualifiers source 1..370/organism＝″Oryctolaguscuniculus″/db_xref＝″taxon:9986″/tissue_type＝″liver″Protein 1..370/product＝″Surfactant protein B(SP-B)″CDS 1..370/gene＝″SP-B″/coded_by＝″join(U40853.1:2194..2263，U40853.1:2591..2718，U40853.1:2941..3012，U40853.1:3257..3382，U40853.1:3590..3727，U40853.1:3925..4014，U40853.1:6043..6226，U40853.1:6421..6581，U40853.1:7266..7346，U40853.1:7829..7891)″/note＝″Surfactant protein B(SP-B)is a keycomponent of lung surfactant，a surface a ctive material secreted by type ll epithelial cells oflung alveolus；SP-B maintains biophysical properties and physiological function of surfactant；Pulmonary surfactant associated protein″ORIGIN 1 makshlppwl lllllptlcg pgtavwatsp lacaqgpefw cqsleqalqc kalghclqev61 wghvgaddlc qecqdivnil tkmtkeaifq dtirkflehe cdvlplkllv pqchhvldvy121 fpltityfls qinakaicqh lglcqpgspe ppldplpdkl vlptllgalp akpgphtqdl181 saqrfpiplp lcwlcrtllk riqamipkgv lamavaqvch vvplvvggic qclaerytvi241 llevllghvl pqlvcglvlr cssvdsigqv pptlealpge wlpqdpecpl cmsvttqarn301 iseqtrpqav yhaclssqld kqeceqfvel htpqllslls rgwdaraicq algacvatls361 plqciqsphfSEQ ID NO1121901176A surfactant protein A gi|382753|prf||1901176A[382753]FEATURES Location/Qualifiers source 1..247/organism＝″Oryctolaguscuniculus″/db_xref＝″taxon:9986″ORIGIN 1 mlllslaltl isapasdtcd tkdvcigspg ipgtpgshgl pgrdgrdgvk gdpgppgpmg61 ppggmpglpg rdgligapgv pgergdkgep gergppglpa yldeelqatl helrhhalqs121 igvlslqgsm kavgekifst ngqsvnfdai revcaraggr iaivkevprs leeneaiasr181 ntyaylglae gptagdfyyl dgdpvnytnw ypgeprgqgr ekcvemytdg kwndknclqy241 rlvicefSEQ ID NO113CAA53510 lung surfactant protein D[Bos taurus]gi|415939|emb|CAA53510.|1[415939]sig_peptide 1..20mat_peptide 21..369/product＝″lung surfactant protein D″CDS 1..369/coded_by＝″X75911.1:102..1211″/db_xref＝″SWISS-PROT:P35246″ORIGIN 1 mlllplsvll lltqpwrslg aemkiysqkt manactlvmc sppedglpgr dgrdgregpr61 gekgdpgspg pagragmpgp agpiglkgdn gsagepgpkg dtgppgppgm pgpagregps121 gkqgsmgppg tpgpkgdtgp kggvgapgiq gspgpaglkg ergapgepga pgragapgpa181 gaigpqgpsg argppglkgd rgtpgergak gesglaevna lrqnvgileg qlqrlqnafs241 qykkamlfpn grsvgekifk tvgsektfqd aqqictqagg qlpsprsgae nealtqlata301 qnkaaflsms dtrkegtfiy ptgeplvysn wapqepnndg gsencveifp ngkwndkvcg361 eqrlvicefSEQ ID NO114CAA53511 collectin-43[Bos taurus]gi|499385|emb|CAA53511.1|[499385]FEATURES Location/Qualifiers source 1..301/organism＝″Bostaurus″/db_xref＝″taxon:9913″/tissue_type＝″liver″/clone_lib＝″lambda gt 11″Protein 1..301/product＝″collectin-43″mat_peptide 1..301/product＝″collectin-43″CDS 1..301/coded_by＝″X75912.1:＜1..906″/db_xref＝″SWISS-PROT:P42916″ORIGIN 1 eemdvyxekt ltdpctlvvc appadslrgh dgrdgkegpq gekgdpgppg mpgpagregp61 sgrqgsmgpp gtpgpkgepg peggvgapgm pgspgpaglk gergapgpgg aigpqgpsga121 mgppglkgdr gdpgekgarg etsvlevdtl rqrmrnlege vqrlqnivtq yrkavlfpdg181 qavgekifkt agavksysda eqlcreakgq lasprssaen eavtqlvrak nkhaylsmnd241 iskegkftyp tggsldysnw apgepgnrak degpenclei ysdgnwndie creerlvice301 fSEQ ID NO115CAA46152 lung surfactant protein D[Homo sapiens]gi|34767|emb|CAA46152.1|[34767]sig_peptide 1..20mat_peptide 21..375/product＝″lung surfactant protein D″CDS1..375/gene＝″hsp-D″/coded_by＝″X65018.1:172..1299″/db_xref＝″SWISS-PROT:P35247″ORIGIN 1 mllfllsalv lltqplgyle aemktyshrt tpsactlvmc ssvesglpgr dgrdgregpr61 gekgdpglpg aagqagmpgq agpvgpkgdn gsvgepgpkg dtgpsgppgp pgvpgpagre121 gplgkqgnig pqgkpgpkge agpkgevgap gmqgsagarg lagpkgergv pgergvpgna181 gaagsagamg pqgspgargp pglkgdkgip gdkgakgesg lpdvaslrqq vealqgqvqh241 lqaafsqykk velfpngqsv gekifktagf vkpfteaqll ctqaggqlas prsaaenaal301 qqlvvaknea aflsmtdskt egkftyptge slvysnwapg epnddggsed cveiftngkw361 ndracgekrl vvcefSEQ ID NO116AAA92788 lung surfactant protein C[Rattus norvegicus]gi|595282|gb|AAA92788.1|[595282]FEATURES Location/Qualifiers source 1..194/organism＝″Rattusnorvegicus″/db_xref＝″taxon:10116″/clone＝″sp-c″/tissue_type＝″liver″Protein 1..194/product＝″lung surfactant protein C″CDS 1..194/gene＝″sp-c″/coded_by＝″join(U07796.1:1673..1714，U07796.1:2841..2999，U07796.1:3252..3377，U07796.1:3598..3707，U07796.1:4053..4200)″ORIGIN 1 mdmgskevlm esppdystgp rsqfripccp vhlkllivv vvvvlvvvvi vgallmglhm61 sqkhtemvle msiggapetq krlalsehtd tiatfsigst givlydyqrl ltaykpapgt121 ycyimkmape sipslealar kfknfqakss tptsklgqee ghsagsdsds sgrdlaflgl181 avstlcgvlp lyyiSEQ ID NO117AAA31468 surfactant protein A[Oryctolagus cuniculus]gi|431446|gb|AAA31468.1|[431446]FEATURES Location/Qualifiers source 1..247/organism＝″Oryctolaguscuniculus″/strain＝″New ZealandWhite″/db_xref＝″taxon:9986″/tissue_type＝″liver″/dev_stage＝″adult″Protein 1..247/product＝″surfactant protein A″CDS 1..247/coded_by＝″join(L19387.1:3864..4032，L19387.1:4241..4360.L19387.1:5010..5087，L19387.1:5533..5909)″ORIGIN 1 mlllslaltl isapasdtcd tkdvcigspg ipgtpgshgl pgrdgrdgvk gdpgppapwa61 ppggmpglpg rdgligapgv pgergdkgep gergppglpa yldeelqatl helrhhalqs121 igvlslqgsm kavgekifst ngqsvnfdai revcaraggr iavprsleen eaiasivker181 ntyaylglae gptagdfyyl dgdpvnytnw ypgeprgqgr ekcvemytdg kwndknclqy241 rlvicef结合甘露糖的凝集素SEQ ID NO1NP_034897 mannan-binding lectin serine protease 2[Mus musculus]gi|6754642|ref|NP_034897.1|[6754642]sig_peptide 1..15mat_peptide 16..185/product＝″mannan-binding lectin serine protease 2″Region 28..137/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein″/note＝″CUB″/db_xref＝″CDD:smart00042″Region 28..134/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 138..180/region_name＝″Calcium-binding EGF-likedomain″/note＝″EGF_CA″/db_xref＝″CDD:smart00179″variation 172/allele＝″I″/allele＝″V″/db_xref＝″dbSNP:3167338″CDS1..185/gene＝″Masp2″/coded_by＝″NM_010767.1:32..589″/db_xref＝″LocusID:17175″/db_xref＝″MGD:1330832″ORIGIN 1 mrlliflgll wslvatllgs kwpepvfgrl vspgfpekya dhqdrswtlt appgyrlrly61 fthfdlelsy rceydfvkls sgtkvlatlc gqestdteqa pgndtfyslg pslkvtfhsd121 ysnekpftgf eafyaaedvd ecrvslgdsv pcdhychnyl ggyycscrag yvlhqnkhtc181 seqslSEQ ID NO2AAH10760 Similar to mannose binding lectin，serum(C)[Mus musculus]gi|14789670|gb|AAH10760.1|[14789670]source 1..244/organism＝″Musmusculus″/strain＝″FVB/N″/db_xref＝″taxon:10090″/clone＝″MGC:18500IMAGE:4212216″/tissue_type＝″Liver，normal.5 month old malemouse.″/clone_lib＝″NCI_CGAP_Li9″/lab_host＝″DH10B″/note＝″Vector:pCMV-SPORT6″Protein 1..244/product＝″Similar to mannose binding lectin，serum(C)″CDS 1..244/coded_by＝″BC010760.1:192..926″ORIGIN 1 msiftslll cvvtvvyaet ltegvqnscp vvtcsspgln gfpgkdgrdg akgekgepgq61 glrglqgppg kvgptgppgn pglkgavgpk gdrgdraefd tseidseiaa lrselralrn121 wvlfslsekv gkkyfvssvk kmsldrvkal csefqgsvat prnaeensai qkvakdiayl181 gitdvrvegs fedltgnrvr ytnwndgepn ntgdgedcvv ilgngkwndv pcsdsflaic241 efsdSEQ ID NO3AAH21762 mannose binding lectin，liver(A)[Mus musculus]gi|8256010|gb|AAH21762.1][18256010]source 1..239/organism＝″Musmusculus″/strain＝″FVB/N″/db_xref＝″taxon:10090″/clone＝″MGC:30242IMAGE:5132514″/tissue_type＝″Liver，normal.5 month old malemouse.″/clone_lib＝″NCI_CGAF_Li9″/lab_host＝″DH10B″/note＝″Vector:pCMV-SPORT6″Protein 1..239/product＝″mannose binding lectin，liver(A)″/db_xref＝″LocusID:17194″CDS 1..239/coded_by＝″BC021762.1:75..794″/db_xref＝″LocusID:17194″ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedtlkt csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr aieeklanme aeirilkskl qltnklhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng lwndiscqas fkavcefpaSEQ ID NO4Q9NPY3 Complement component C1q receptor precursor(Complement component 1，qsubcomponent，receptor 1)(C1qRp)(C1qR(p))(C1q/MBL/SPA receptor)(CD93 antigen)(CDw93)gi|21759074|sp|Q9NPY3|CD93_HUMAN[21759074]source 1..652/organism＝″Homo sapiens″/db_xref＝″taxon:9606gene 1..652/gene＝″C1QR1″/note＝″CD93″Protein 1..652/gene＝″C1QR1″/pnoduct＝″Complement component C1q receptor p recursor″Region 1..21/gene＝″C1QR1″/region_name＝″Signal″Region 22..652/gene＝″C1QR1″/region_name＝″Mature chain″/note＝″COMPLEMENTCOMPONENT C1Q RECEPTOR.Region 22/gene＝″C1QR1″/region_name＝″Conflict″/note＝″T-＞V(IN AA SEQUENCE).″Region 24..580/gene＝″C1QR1″/region_name＝″Domain″/note＝″EXTRACELLULAR(POTENTIAL).”Region 32..174/gene＝″C1QR1″/region_name＝″Domain″/note＝″C-TYPE LECTIN.″Region 36/gene＝″C1QR1″/region_name＝″Conflict″/note＝″C-＞T(IN AA SEQUENCE).″Region 38..39/gene＝″C1QR1″/region_name＝″Conflict″/note＝″TA-＞RI(IN AASEQUENCE).″Region 155/gene＝″C1QR1″/region_name＝″Conflict″/note＝″S-＞N(IN REF.1).″Region 186/gene＝″C1QR1″/region_name＝″Conflict″/note＝″G-＞A(IN AA SEQUENCE).″Region 260..301/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 1.″Bond bond(264，275)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(271，285)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(287，300)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 302..344/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 2.″Bond bond(306，317)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(311，328)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 318/gene＝″C1QR1″/region_name＝″Variant″/note＝″V-＞A./FTId＝VAR_013573.″Site 325/gene＝″C1QR1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Bond bond(330，343)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 345..384/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 3，CALCIUM-BINDING(POTENTIAL).″Bond bond(349，358)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(354，367)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(369，383)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 385..426/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 4，CALCIUM-BINDING(POTENTIAL).″Bond bond(389，400)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(396，409)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(411，425)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 427..468/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 5，CALCIUM-BINDING(POTENTIAL).″Bondbond(431，443)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(439，452)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(454，467)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 492/gene＝″C1QR1″/region_name＝″Conflict″/note＝″S-＞A(IN AA SEQUENCE).″Region 496/gene＝″C1QR1″/region_name＝″Conflict″/note＝″R-＞Q(IN AA SEQUENCE).″Region 504/gene＝″C1QR1″/region_name＝″Conflict″/note＝″R-＞G(IN AA SEQUENCE).″Region 541/gene＝″C1QR1″/region_name＝″Conflict″/note＝″P-＞S(IN REF.1).″Region 581..601/gene＝″C1QR1″/region_name＝″Transmembraneregion″/note＝″POTENTIAL.″Region 594..601/gene＝″C1QR1″/region_name＝″Domain″/note＝″POLY-LEU.″Region 602..652/gene＝″C1QR1″/region_name＝″Domain″/note＝″CYTOPLASMIC(POTENTIAL).″ORIGIN 1 matsmgllll llllltqpga gtgadteavv cvgtacytah sgklsaaeaq nhcnqnggnl61 atvkskeeaq hvqrvlaqll rreaaltarm skfwiglqre kgkcldpslp lkgfswvggg121 edtpysnwhk elrnsciskr cvsllldlsq pllpsrlpkw segpcgspgs pgsniegfvc181 kfsfkgmcrp lalggpgqvt yttpfqttss sleavpfasa anvacgegdk detqshyflc241 kekapdvfdw gssgplcvsp kygcnfnngg chqdcfeggd gsflcgcrpg frllddlvtc301 asrnpcsssp crggatcvlg phgknytcrc pqgyqldssq ldcvdvdecq dspcaqecvn361 tpggfrcecw vgyepggpge gacqdvdeca lgrspcaqgc tntdgsfhcs ceegyvlage421 dgtqcqdvde cvgpggplcd slcfntqgsf hcgclpgwvl apngvsctmg pvslgppsgp481 pdeedkgeke gstvpraata sptrgpegtp katpttsrps lssdapitsa plkmlapsgs541 pgvwrepsih hataasgpqe paggdssvat qnndgtdgqk lllfyilgtv vaillllala601 lgllvyrkrr akreekkekk pqnaadsysw vperaesram enqysptpgt dcSEQ ID NO5O89103 Complement component C1q receptor precursor(Complement component 1，qsubcomponent，receptor 1)(C1qRp)(C1qR(p))(C1q/MBL/SPA receptor)(CD93 antigen)(Cellsurface antigen AA4)(Lymphocyte antigen 68)gi|21541998|sp|O89103|CD93_MOUSE[21541998source 1..644/organisn＝″Mus musculus″/db_xref＝″taxon:10090″gene 1..644/gene＝″C1QR1″/note＝″CD93；C1QRP；LY68；AA4″Protein 1..644/gene＝″C1QR1″/product＝″Complement component C1q receptor precursor″Region 1..22/gene＝″C1QR1″/region_name＝″Signal″/note＝″POTENTIAL″Region 23..644/gene＝″C1QR1″/region_name＝″Mature chain″/note＝″COMPLEMENTCOMPONENT C1Q RECEPTOR.″Region 23..572/gene＝″C1QR1″/region_name＝″Domain″/note＝″EXTRACELLULAR(POTENTIAL).″Region 31..173/gene＝″C1QR1″/region_name＝″Domain″/note＝″C-TYPE LECTIN.″Site 102/gene＝″C1QR1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Region 257..298/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 1.″Bond bond(261，272)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(268，282)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(284，297)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 299..341/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 2.″Bond bond(303，314)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(308，325)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Site 322/gene＝″C1QR1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Bond bond(327，340)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 342..381/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 3，CALCIUM-BINDING(POTENTIAL).″Bond bond(346，355)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(351，364)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(366，380)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 382..423/gene＝″C1QR1″/region name＝″Domain″/note＝″EGF-LIKE 4，CALCIUM-BINDING(POTENTIAL).″Bond bond(386，397)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(393，406)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(408，422)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 424..465/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 5，CALCIUM-BINDING(POTENTIAL).″Bond bond(428，440)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(436，449)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY″Bond bond(451，464)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 573..593/gene＝″C1QR1″/region_name＝″Transmembraneregion″/note＝″POTENTIAL.″Region 594..644/gene＝″C1QR1″/region_name＝″Domain″/note＝″CYTOPLASMIC(POTENTIAL).″ORIGIN 1 maistglfll lgllgqpwag aaadsqavvc egtacytahw gklsaaeaqh rcnenggnla61 tvkseeearh vqqaltqllk tkapleakmg kfwiglqrek gnctyhdlpm rgfswvggge121 dtaysnwyka sksscifkrc vslildlslt phpshlpkwh espcgtpeap gnsiegflck181 fnfkgmcrpl alggpgrvty ttpfqattss leavpfasva nvacgdeaks ethyflcnek241 tpgifhwgss gplcvspkfg csfnnggcqq dcfeggdgsf rcgcrpgfrl lddlvtcasr301 npcssnpctg ggmchsvpls enytcrcpsg yqldssqvhc vdidecqdsp caqdcvntlg361 sfhcecwvgy qpsgpkeeac edvdecaaan spcaqgcint dgsfycscke gyivsgedst421 qcedidecsd angnpcdslc fntdgsfrcg cppgwelapn gvfcsrgtvf selparppqk481 ednddrkest mpptempssp sgskdvsnra qttglfvqsd iptasvplei eipsevsdvw541 felgtylptt sghskpthed svsahsdtdg qnlllfyilg tvvaislllv lalgiliyhk601 rrakkeeike kkpq naadsy swvperaesq apenqysptp gtdcSEQ ID NO6P09871 Complement C1s component precursor(C1 esterase)gi|115205|sp|P09871|C1S_HUMAN[115205]source 1..688/organism＝″Homo sapiens″/db_xref＝″taxon:9606″gene 1..688/gene＝″C1S″Protein 1..688/gene＝″C1S″/product＝″Complement C1s componentprecursor″/EC_number＝″3.4.21.42″Region 1..15/gene＝″C1S″/region_name＝″Signal″Region 16..437/gene＝″C1S″/region_name＝″Mature chain″/note＝″COMPLEMENT C1SHEAVY CHAIN.″Region 16..130/gene＝″C1S″/region_name＝″Domain″/note＝″CUB 1.″Bond bond(65，83)/gene＝″C1S″/bond_type＝″disulfide″Region 131..172/gene＝″C1S″/region_name＝″Domain″/note＝″EGF-LIKE，CALCIUM-BINDING(POTENTIAL).″Bond bond(135，147)/gene＝″C1S″/bond_type＝″disulfide″Bond bond(143，156)/gene＝″C1S″/bond_type＝″disulfide″Site 149/gene＝″C1S″/site_type＝″hydroxylation″/note＝″(PROBABLE).″Bond bond(158，171)/gene＝″C1S″/bond_type＝″disulfide″Site 174/gene＝″C1S″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...).″Region 175..290/gene＝″C1S″/region_name＝″Domain″/note＝″CUB 2.″Bond bond(175，202)/gene＝″C1S″/bond_type＝″disulfide″Bondbond(234，251)/gene＝″C1S″/bond_type＝″disulfide″Region293..355/gene＝″C1S″/region_name＝″Domain″/note＝″SUSHI 1.″Bond bond(294，341)/gene＝″C1S″/bond_type＝″disulfide″Region 294/gene＝″C1S″/region_name＝″Conflict″/note＝″C-＞K(IN REF.6).″Bond bond(321，354)/gene＝″C1S″/bond_type＝″disulfide″Region 358..422/gene＝″C1S″/region_name＝″Domain″/note＝″SUSHI 2.″Bond bond(359，403)/gene＝″C1S″/bond_type＝″disulfide″Bond bond(386，421)/gene＝″C1S″/bond_type＝″disulfide″Site 406/gene＝″C1S″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...).″Bond bond(425，549)/gene＝″C1S″/bond_type＝″disulfide″/note＝″INTERCHAIN.″Region 438..688/gene＝″C1S″/region_name＝″Mature chain″/note＝″COMPLEMENT C1SLIGHT CHAIN.″Region 438..688/gene＝″C1S″/region_name＝″Domain″/note＝″SERINEPROTEASE″Site 475/gene＝″C1S″/site_type＝″active″/note＝″CHARGE RELAY SYSTEM.″Region 513/gene＝″C1S″/region_name＝″Conflict″/note＝″G-＞GG(IN REF.5).″Site 529/gene＝″C1S″/site_type＝″active″/note＝″CHARGE RELAY SYSTEM.″Region 573/gene＝″C1S″/region_name＝″Conflict″/note＝″T-＞A(IN REF.7).″Bond bond(595，618)/gene＝″C1S″/bond_type＝″disulfide″Bond bond(628，659)/gene＝″C1S″/bond_type＝″disulfide″Site 632/gene＝″C1S″/site_type＝″active″/note＝″CHARGE RELAY SYSTEM.″Region 645..646/gene＝″C1S″/region_name＝″Conflict″/note＝″TK-＞GR(IN REF.7).″ORIGIN 1 mwcivlfsllawvyaeptmy geilspnypq aypseveksw dievpegygi hlyfthldie61 lsencaydsv qiisgdteeg rlcgqrssnn phspiveefq vpynklqvif ksdfsneerf121 tgfaayyvat dinectdfvd vpcshfcnnf iggyfcscpp eyflhddmkn cgvncsgdvf181 taligeiasp nypkpypens rceyqirlek gfqvvvtlrr edfdveaads agncldslvf241 vagdrqfgpy cghgfpgpln ietksnaldi ifqtdltgqk kgwklrvhgd pmpcpkedtp301 nsvwepakak yvfrdvvqit cldgfevveg rvgatsfyst cqsngkwsns klkcqpvdcg361 ipesiengkv edpestlfgs virytceepy yymengggge yhcagngswv nevlgpelpk421 cvpvcgvpre pfeekqriig gsdadiknfp wqvffdnpwa ggalineywv ltaahvvegn481 reptmyvgst svqtsrlaks kmltpehvfi hpgwkllevp egrtnfdndi alvrlkdpvk541 mgptvspicl pgtssdynlm dgdlglisgw grtekrdrav rlkaarlpva plrkckevkv601 ekptadaeay vftpnmicag gekgmdsckg dsggafavqd pndktkfyaa glvswgpqcg661 tyglytrvkn yvdwimktmq enstpredSEQ ID NO7NP_036204 complement component 1，q subcomponent，receptor 1；complement componentC1q receptor[Homo sapiens]gi|6912282|ref|NP_036204.1|[6912282]source 1..652/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″20″/map＝″20p11.21″Protein 1..652/product＝″complement component 1，q subcompontent，receptor1″/note＝″complement component C1q receptor″Region 32..130/region_name＝″smart00034，CLECT，C-type lectin(CTL)orcarbohydrate-recognition domain(CRD)；Many of these domains function ascalcium-dependent carbohydrate binding modules″Region 47..128/region_name＝″pfam00059，lectin_c，Lectin C-type domain.This familyincludes both long and short form C-type″Region 385..426/region_name＝″smart00179，EGF_CA，Calcium-binding EGF-like domain″CDS 1..652/gene＝″C1QR1″/coded_by＝″NM_012072.2:149..2107″/note＝″Clq/MBL/SPAreceptor″/db_xref＝″LocusID:22918″/db_xref＝″MIM:120577″ORIGIN 1 matsmgllll llllltqpga gtgadteavv cvgtacytah sgklsaaeaq nhcnqnggnl61 atvkskeeaq hvqrvlaqll rreaaltarm skfwiglqre kgkcldpslp lkgfswvggg121 edtpysnwhk elrnsciskr cvsllldlsq pllpnrlpkw segpcgspgs pgsniegfvc181 kfsfkgmcrp lalggpgqvt yttpfqttss sleavpfasa anvacgegdk detqshyflc241 kekapdvfdw gssgplcvsp kygcnfnngg chqdcfeggd gsflcgcrpg frllddlvtc301 asrnpcsssp crggatcvlg phgknytcrc pqgyqldssq ldcvdvdecq dspcaqecvn361 tpggfrcecw vgyepggpge gacqdvdeca lgrspcaqgc tntdgsfhcs ceegyvlage421 dgtqcqdvde cvgpggplcd slcfntqgsf hcgclpgwvl apngvsctmg pvslgppsgp481 pdeedkgeke gstypraata sptrgpegtp katpttsrps lssdapitsa plkmlapsgs
541 sgvwrepsih hataasgpqe paggdssvat qnndgtdgqk lllfyilgtv vaillllala601 lgllvyrkrr akreekkekk pqnaadsysw vperaesram enqysptpgt dcSEQ ID NO8NP_000233 soluble mannose-binding lectin precursor；mannose-binding lectin；mannosebinding protein；Mannose-binding lectin 2，soluble(opsonic defect)[Homo sapiens]gi|4557739|ref|NP_000233.1|[4557739]sig_peptide 1..20mat_peptide 21..248/product＝″soluble mannose-binding lectin″variation 54/allele＝″D″/allele＝″G″/db_xref＝″dbSNP:1800450″variation 57/allele＝″E″/allele＝″G″/db_xref＝″dbSNP:1800451″Region 134..245/region_name＝″smart00034，CLECT，C-type lectin(CTL)orcarbohydrate-recognition domain(CRD)；Many of these domains function ascalcium-dependent carbohydrate binding modules″Region 144..246/region_name＝″pfam00059，lectin_c，Lectin C-type domain.This familyincludes both long and short form C-type″CDS1..248/gene＝″MBL2″/coded_by＝″NM_000242.1:66..812″/db_xref＝″LocusID:4153″/db_xref＝″MIM:154545″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO9P11 226 Mannose-binding protein C precursor(MBP-C)(MBP1)(Mannan-binding protein)(Mannose-binding lectin)gi|126676|sp|P11226|MABC_HUMAN[126676]source 1..248/organism＝″Homo sapiens″/db_xref＝″taxon:9606″gene 1..248/gene＝″MBL2″/note＝″MBL″Protein 1..248/gene＝″MBL2″/product＝″Mannose-binding protein C precursor″Region 1..20/gene＝″MBL2″/region_name＝″Signal″Region 21..248/gene＝″MBL2″/region_name＝″Mature chain″/note＝″MANNOSE-BINDINGPROTEIN C.″Region 21..41/gene＝″MBL2″/region_name＝″Domain″/note＝″CYS-RICH.″Region 24/gene＝″MBL2″/region_name＝″Variant″/note＝″T-＞A(INCHINESE)./FTId＝VAR_013294.″Region 42..99/gene＝″MBL2″/region_name＝″Domain″/note＝″COLLAGEN-LIKE.″Site 47/gene＝″MBL2″/site_type＝″hydroxylation″Region 52/gene＝″MBL2″/region_name＝″Variant″/note＝″R-＞C(IN 0.05％ OF EUROPEANAND AFRICAN POPULATIONS)./FTId＝VAR_008543.″Region 54/gene＝″MBL2″/region_name＝″Variant″/note＝″G-＞D(IN CAUCASIAN ANDCHINESE POPULATIONS)./FTId＝VAR_004182.″Region 57/gene＝″MBL2″/region_name＝″Variant″/note＝″G-＞E(IN WEST AFRICANPOPULATION)./FTId＝VAR_004183.″Site 73/gene＝″MBL2″/site_type＝″hydroxylation″Site 79/gene＝″MBL2″/site_type＝″hydroxylation″Site 82/gene＝″MBL2″/site_type＝″hydroxylation″Site 88/gene＝″MBL2″/site_type＝″hydroxylation″Region 109/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 110..129/gene＝″MBL2″/region_name＝″Helical region″Region 130/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 132..134/gene＝″MBL2″/region_name＝″Beta-strand region″Region 135..136/gene＝″MBL2″/reqion_name＝″Hydrogen bonded turn″Region 137..147/gene＝″MBL2″/region_name＝″Beta-strand region″Region 148..157/gene＝″MBL2″/region_name＝″Helical region″Region 153..246/gene＝″MBL2″/region_name＝″Domain″/note＝″C-TYPE LECTIN(SHORTFORM).″Bond bond(155，244)/gene＝″MBL2″/bond_type＝″disulfide″Region 158..159/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 161..162/gene＝″MBL2″/region_name＝″Beta-strand region″Region 168..177/gene＝″MBL2″/region_name＝″Helical region″Region 182..187/gene＝″MBL2″/region_name＝″Beta-strand region″Region 192..193/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 196..197/gene＝″MBL2″/region_name＝″Beta-strand region″Region 198..199/gene＝″MBL2″/region_name＝″Hydrogen bonded tLrn″Region 202/gene＝″MBL2″/region_name＝″Beta-strand region″Region 208/gene＝″MBL2″/region_name＝″Beta-strand region″Region 210..211/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 216..218/gene＝″MBL2″/region_name＝″Helical region″Bond bond(222，236)/gene＝″MBL2″/bond_type＝″disulfide″Region 222..225/gene＝″MBL2″/region_name＝″Beta-strand region″Region 227..228/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 231..234/gene＝″MBL2″/region_name＝″Beta-strand region″Region 236..237/gene＝″MBL2″/region_name＝″Hydrogen bonded turn″Region 239..248/gene＝″MBL2″/region_name＝″Beta-strand region″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO10Q9ET61 Complement component C1q receptor precursor(Complement component 1，qsubcomponent，receptor 1)(C1qRp)(C1qR(p))(C1q/MBL/SPA receptor)(CD93 antigen)(Cellsurface antigen AA4)gi|21541989|sp|Q9ET61|CD93_RAT[21541989]source 1..643/organism＝″Rattus norvegicus″/db_xref＝″taxon:10116″gene 1..643/gene＝″C1QR1″/note＝″CD93；C1QRP2″Protein 1..643/gene＝″C1QR1″/product＝″Complement component C1q receptor precursor″Region 1..23/gene＝″C1QR1″/region_name＝″Signal″/note＝″POTENTIAL.″Region 24..643/gene＝″C1QR1″/region_name＝″Mature chain″/note＝″COMPLEMENTCOMPONENT C1Q RECEPTOR.″Region 24..571/gene＝″C1QR1″/region_name＝″Domain″/note＝″EXTRACELLULAR(POTENTIAL).″Region 31..173/gene＝″C1QR1″/region_name＝″Domain″/note＝″C-TYPE LECTIN″Region 257..298/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 1.″Bond bond(261，272)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(268，282)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(284，297)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 299..341/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 2.″Bond bond(303，314)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(308，325)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Site 322/gene＝″C1QR1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Bond bond(327，340)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 342..381/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 3，CALCIUM-BINDING(POTENTIAL).″Bond bond(346，355)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(351，364)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(366，380)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 382..423/gene＝″C1QR1″/region_name＝″Domain″/note＝＝″EGF-LIKE 4，CALCIUM-BINDING(POTENTIAL).″Bond bond(386，397)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY″Bond bond(393，406)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(408，422)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Region 417/gene＝″C1QR1″/region_name＝″Conflict″/note＝″E-＞K(IN REF.2).″Region 424..462/gene＝″C1QR1″/region_name＝″Domain″/note＝″EGF-LIKE 5，CALCIUM-BINDING(POTENTIAL).″Bond bond(428，437)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(433，446)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Bond bond(448，461)/gene＝″C1QR1″/bond_type＝″disulfide″/note＝″BY SIMILARITY.″Site 498/gene＝″C1QR1″/site_type＝″glycosylation″/note＝″N-LINKED(GLCNAC...)(POTENTIAL).″Region 572..592/gene＝″C1QR1″/region_name＝″Transmembraneregion″/note＝″POTENTIAL.″Region 593..643/gene＝″C1QR1″/region_name＝″Domain″/note＝″CYTOPLASMIC(POTENTIAL).″ORIGIN 1 mvtstgllll lgllgqlwag aaadseavvc egtacytahw gklsaaeaqh rcnenggnla61 tvkseeearh vqealaqllk tkapsetkig kfwiglqrek gkctyhdlpm kgfswvggge121 dttysnwyka skssciskrc vslildlslk phpshlpkwh espcgtpdap gnsiegflck181 fnfkgmcspl alggpgqlty ttpfqattss lkavpfasva nvvcgdeaes ktnyylcket241 tagvfhwgss gplcvspkfg csfnnggcqq dcfeggdgsf rcgcrpgfrl lddlvtcasr301 npcssnpctg ggmchsvpls enytchcprg yqldssqvhc vdidecedsp cdqecintpg361 gfhcecwvgy qssgske6ac edvdectaay spcaqgctnt dgsfycscke gyimsgedst421 qcedideclg npcdtlcint dgsfrcgcpa gfelapngvs ctrgsmfsel parppqkedk481 gdgkestvpl tempgslngs kdvsnraqtt dlsiqsdsst asvpleievs seasdvwldl541 gtylpttsgh sqpthedsvp ahsdsdtdgq klllfyilgt vvaislllal alglliylkr601 kakkeeikek kaqnaadsys wiperaesra penqysptpg tdcSEQ ID NO11NP_006601 mannan-binding lectin se rine protease 2，isoform 1 precursor；MBL-associatedplasma protein of 19 kD；small MBL-associated protein[Homo sapiens]gi|21264363|ref|NP_006601.2|[21264363]sig_peptide 1..15mat_peptide 16..444/product＝″mannan-binding lectin serine protease 2，isoform 1，chain A″Region 28..136/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″Region 28..134/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 138..180/region_name＝″Calcium-binding EGF-likedomain″/note＝″EGF_CA″/db_xref＝″CDD:smart00179″variation 155/allele＝″H″/allele＝″R″/db_xref＝″dbSNP:2273343″Region 184..295/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″Region 184..293/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431”Region 300..361/region_name＝″Domain abundant in complement controlproteins″/note＝″CCP″/db_xref＝″CDD:smart00032”Region 300..361/region_name＝″Sushi domain(SCRrepeat)″/note＝″sushi″/db_xref＝″CDD:pfam00084″Region 366..430/region_name＝″Domain abundant in complement controlproteins″/note＝″CCP″/db_xref＝″CDD:smart00032″Region 366..430/region_name＝″Sushi domain(SCRrepeat)″/note＝″sushi″/db_xref＝″CDD:pfam00084″variation 377/allele＝″A″/allele＝″V″/db_xref＝″dbSNP:2273346″Region 444..679/region_name＝″Trypsin-like serineprotease″/note＝″Tryp_SPc″/db_xref＝″CDD:smart00020″mat peptide445..686/product＝″mannan-binding lectin serine protease 2，isoform 1，chain B″Region 445..679/region_name＝″Trypsin″/note＝″trypsin″/db_xref＝″CDD:pfam00089″CDS1..686/gene＝″MASP2″/coded_by＝″NM_006610.2:22..2082″/db_xref＝″LocusID:10747″/db_xref＝″MIM:6051 02″ORIGIN 1 mrlltllgll cgsvatplgp kwpepvfgrl aspgfpgeya ndqerrwtlt appgyrlrly61 fthfdlelsh lceydfvkls sgakvlatlc gqestdtera pgkdtfyslg sslditfrsd121 ysnekpftgf eafyaaedid ecqvapgeap tcdhhchnhl ggfycscrag yvlhrnkrtc181 salcsgqvft qrsgelsspe yprpypklss ctysisleeg fsvildfves fdvethpetl241 cpydflkiqt dreehgpfcg ktlphrietk sntvtitfvt desgdhtgwk ihytstaqpc301 pypmappngh vspvqakyil kdsfsifcet gyellqghlp lksftavcqk dgswdrpmpa361 csivdcgppd dlpsgrveyi tgPgvttyka viqysceetf ytmkvndgky vceadgfwts421 skgekslpvc epvcglsart tggriyggqk akpgdfpwqv lilggttaag allydnwvlt481 aahavyeqkh dasaldirmg tlkrlsphyt qawseavfih egythdagfd ndialiklnn541 kvvinsnitp iclprkeaes fmrtddigta sgwgltqrgf lernlmyvdi pivdhqkcta601 ayekppyprg svtanmlcag lesggkdscr gdsggalvfl dseterwfvg givswgsmnc661 geagqygvyt kvinyipwie niisdfSEQ ID NO12NP_631947 mannan-binding lectin serine protease 2，isoform 2 precursor；MBL-associatedplasma protein of 19 kD；small MBL-associated protein[Homo sapiens]gi|21264361|ref|NP_631947.1|[21264361]sig_peptide 1..15mat_peptide 16..185/product＝″mannan-binding lectin serine protease 2，isoform 2″Region 28..136/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″Region 28..134/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 138..180/region_name＝″Calcium-binding EGF-likedomain″/note＝″EGF_CA″/db_xref＝″CDD:smart00179″variation 155/allele＝″H″/allele＝″R″/db_xref＝″dbSNP:2273343″CDS1..185/gene＝″MASP2″/coded_by＝″NM_139208.1:22..579″/db_xref＝″LocusID:10747″/db_xref＝″MIM:605102″ORIGIN 1 mrlltllgll cgsvatplgp kwpepvfgrl aspgfpgeya ndqerrwtlt appgyrlrly61 fthfdlelsh lceydfvkls sgakvlatlc gqestdtera pgkdtfyslg sslditfrsd121 ysnekpftgf eafyaaedid ecqvapgeap tcdhhchnhl ggfycscrag yvlhrnkrtc181 seqslSEQ ID NO13NP_624302 mannan-binding lectin serine protease 1，isoform 2，precursor；protease，serine，5(mannose-binding protein-associated)；manan-binding lectin serine protease-1；Ra-reactivefactor serine protease p100[Homo sapiens]gi|21264359|ref|NP_624302.1|[21264359]sig_peptide 1..19mat_peptide 20..445/product＝″mannan-binding lectin senine protease 1，isoform 2，chain A″variation 21/allele＝″I″/allele＝″T″/db_xref＝″dbSNP:1062049″Region 23..138/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″Region 23..135/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 139..181/region_name＝″Calcium-binding EGF-likedomain″/note＝″EGF_CA″/db_xref＝″CDD:smart00179″Region 185..294/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 190..296/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″variation 235/allele＝″Q″/allele＝″E″/db_xref＝″dbSNP:3203210″variation 258/allele＝″P″/allele＝″A″/db_xref＝″dbSNP:866085″Region 301..362/region_name＝″Domain abundant in complement controlproteins″/note＝″CCP″/db_xref＝″CDD:smart00032″Region 301..362/region_name＝″Sushi domain(SCRrepeat)″/note＝″sushi″/db_xref＝″CDD:pfam00084″Region 367..432/region_name＝″Domain abundant in complement controlproteins″/note＝″CCP″/db_xre＝″CDD:smart00032″Region 367..432/region_name＝″Sushi domain(SCRrepeat)″/note＝″sushi″/db_xref＝″CDD:pfam00084″mat_peptide446..728/product＝″mannan-binding lectin serine protease 1，isoform 2，chain B″Region 449..711/region_name＝″Trypsin-like serineprotease″/note＝″Tryp_SPc″/db_xref＝″CDD:smart00020″Region450..711/region_name＝″Trypsin″/note＝″trypsin″/db_xref＝″CDD:pfam00089″variation 616/allele＝″A″/allele＝″V″/db_xref＝″dbSNP:2461280″Region 661..703/region_name＝″lmmunoglobulin A1protease″/note＝″IGA1″/db_xref＝″CDD:pfam02395″CDS1..728/gene＝″MASP1″/coded_by＝″NM_139125.1:51..2237″/db_xref＝″LocusID:5648″/db_xref＝″MIM:600521″ORIGIN 1 mrwlllyyal cfslskasah tvelnrmfgq iqspgypdsy psdsevtwni tvpdgfrik61 yfmhfnless ylceydyvkv etedqvlatf cgrettdteq tpgqevvlsp gsfmsitfrs121 dfsneerftg fdahymavdv deckeredee lscdhychny iggyycscrf gyilhtdnrt181 crvecsdnlf tqrtgvitsp dfpnpypkss eclytielee gfmvnlqfed ifdiedhpev241 pcpydyikik vgpkvlgpfc gekapepist qshsvlilfh sdnsgenrgw rlsyraagne301 cpelqppvhg kiepsqakyf fkdqvlvscd tgykvlkdnv emdtfqiecl kdgtwsnkip361 tckivdcrap gelehglitf strnnlttyk seikyscqep yykmlnnntg iytcsaqgvw421 mnkvlgrslp tclpecgqps rslpslvkri iggrnaepgl fpwqalivve dtsrvpndkw481 fgsgallsas wiltaahvlr sqrrdttvip vskehvtvyl glhdvrdksg avnssaarvv541 lhpdfniqny nhdialvqlq epvplgphvm pvclprlepe gpaphmlglv agwgisnpnv601 tvdeiissgt rtlsdvlqyv klpvvphaec ktsyesrsgn ysvtenmfca gyyeggkdtc661 lgdsggafvi fddlsqrwvv qglvswggpe ecgskqvygv ytkvsnyvdw vweqmglpqs721 vvepqverSEQ ID NO14NP_001870 mannan-binding lectin serine protease 1，isoform 1，precursor；protease，serine，5(mannose-binding protein-associated)；manan-binding lectin serine protease-1；Ra-reactivefactor serine protease p100[Homo sepiens]gi|21264357|ref|NP_001870.3|[21264357]sig_peptide 1..19mat_peptide 20..448/product＝″mannan-binding lectin serine protease 1，isoform 1，chain A″variation 21/allele＝″I″/allele＝″T″/db_xref＝″dbSNP:1062049″Region 23..138/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″Region 23..135/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 139..181/region_name＝″Calcium-binding EGF-likedomain″/note＝″EGF_CA″/db_xref＝″CDD:smart00179″Region 185..294/region_name＝″CUB domain″/note＝″CUB″/db_xref＝″CDD:pfam00431″Region 190..296/region_name＝″Domain first found in C1r，C1s，uEGF，and bonemorphogenetic protein.″/note＝″CUB″/db_xref＝″CDD:smart00042″variation 235/allele＝″Q″/allele＝″E″/db_xref＝″dbSNP:3203210″variation 258/allele＝″P″/allele＝″A″/db_xref＝″dbSNP:866085″Region 301..362/region_name＝″Domain abundant in complement controlproteins″/note＝″CCP″/db_xref＝″CDD:smart00032″Region 301..362/region_name＝″Sushi domain(SCRrepeat)″/note＝″sushi″/db_xref＝″CDD:pfam00084″Region 367..432/region_name＝″Domain abundant in complement controlproteins″/note＝″CCP″/db_xref＝″CDD:smart00032″Region 367..432/region_name＝″Sushi domain(SCRrepeat)″/note＝″sushi″/db_xref＝″CDD:pfam00084″Region 448..691/region_name＝″Trypsin-like serineprotease″/note＝″Tryp_SPc″/db_xref＝″CDD:smart00020″mat_peptide449..699/product＝″mannan-binding lectin serine protease 1，isoform 1，chain B″Region 449..691/region_name＝″Trypsin″/note＝″trypsin″/db_xref＝″CDD:pfam00089″Region 644..675/region_name＝″lmmunoglobulin A1protease″/note＝″IGA1″/db_xref＝″CDD:pfam02395″CDS1..699/gene＝″MASP1″/coded_by＝″NM_001879.3:51..2150″/db_xref＝″LocusID:5648″/db_xref＝″MIM:600521″ORIGIN 1 mrwlllyyal cfslskasah tvelnnmfgq iqspgypdsy psdsevtwni tvpdgfrikl61 yfmhfnless ylceydyvkv etedqvlatf cgrettdteq tpgqevvlsp gsfmsitfrs121 dfsneerftg fdahymavdv deckeredee lscdhychny iggyycscrf gyilhtdnrt181 crvecsdnlf tqrtgvitsp dfpnpypkss eclytielee gfmvnlqfed ifdiedhpev241 pcpydyikik vgpkvlgpfc gekapepist qshsvlilfh sdnsgenrgw rlsyraagne301 cpelqppvhg kiepsqakyf fkdqvlvscd tgykvlkdnv emdtfqiecl kdgtwsnkip361 tckivdcrap gelehgliif strnnlttyk seikyscqep yykmlnnntg iytcsaqgvw421 mnkvlgrslp tclpvcglpk fsrklmarif ngrpaqkgtt pwiamlshln gqpfcggsll481 gsswiviaah clhqsldped ptlrdsdlls psdfkiilgk hwrlrsdene qhlgvkhttl541 hpqydpntfe ndvalvelle spvlnafvmp iclpegpqqe gamvivsgwg kqflqrfpet601 lmeieipivd hstcqkayap lkkkvtrdmi cagekeggkd acagdsggpm vtlnrergqw661 ylvgivswgd dcgkkdrygv ysyihhnkdw iqrvtgvrnSEQ ID NO15XP_122683 similar to mannose binding lectin，liver(A)[Mus musculus]gi|20872845|ref|XP_122683.1|[20872845]source 1..239/organism＝″Musmusculus″/strain＝″C57BL/6J″/db_xref＝″taxon:10090″/chromosome＝″14″Protein 1..239/product＝″similar to mannose binding lectin，liver(A)″Region 126..236/region_name＝″C-type leciin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smartO0034″Region 135..237/region_name＝″Lectin C-typedomain″/note＝″leciin_c″/db_xref＝″CDD:pfam00059”CDS1..239/gene＝″Mbl1″/coded_by＝″XM_122683.1:10..729″/db_xref＝″LocusID:17194″/db_xref＝″MGD:96923″ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedilki csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr xxxxxxxxxx xxxxxxxxxx xxxxxxhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng lwndiscqas fkavcefpaSEQ ID NO16AAM21196 C-type mannose-binding lectin[Oncorhynchus mykiss]gi[20385163|gb|AAM21196.1|AF363271_1[20385163]source 1..185/organism＝″Oncorhynchus mykiss″/db_xref＝″taxon:8022″Protein 1..185/product＝″C-type mannose-binding lectin″CDS 1..185/gene＝″MBL″/coded_by＝″AF363271.1:25..582″ORIGIN 1 meklaillll sasialgdan ltqllglepl lktkveqttp eaqveavqeg ikegscpsdw61 ytygshcfkf vsiqqsfvds eqnclalggn lasvhslley qfmqaltkda nghlhstwlg121 gfdaikegtw mwsdgsrfdy tnwdtdepnn agegedclhm neasaklwfd vpcewkfasl181 csrrmSEQ ID NO17AAD45377 mannose-binding lectin[Sus scrofa]gi|5566370|gb|AAD45377.1|AF164576_1[5566370]source 1..240/organism＝″Sus scrofa″/db_xref＝″taxon:9823″/tissue_type＝″liver″Protein 1..240/product＝″mannose-binding lectin″CDS 1..240/coded_by＝″AF164576.1:1..723″ORIGIN 1 mslfpslhll llivmtasht etencediqn tclviscdsp ginglpgkdg ldgakgekge61 pgqgliglqg lpgmvgpqgs pgipglpglk gqkgdsgidp gnslanlrse ldnikkwlif121 aqgkqvgkkl yltngkkmsf ngvkalcaqf qasvatptns renqaiqela gteaflgitd181 eytegqfvdl tgkrvryqnw ndgepnnads aehcveilkd gkwndifcss qlsavcefpaSEQ ID NO18NP_034905 mannose binding lectin，liver(A)[Mus musculus]gi|6754654|ref|NP_034905.1|[6754654]source 1..239/organism＝″Musmusculus″/db_xref＝″taxon:10090″/chromosome＝″14″/map＝″14 15.0 cM″Protein 1..239/product＝″mannose binding lectin，liver(A)″misc_feature 19..239/partial/note＝″mature protein based on homology to rat MPB-A″Region 126..236/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 135..237/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS1..239/gene＝″Mbl1″/coded_by＝″NM 010775.1:121..840″/db_xref＝″LocusID:17194″/db_xref＝″MGD:96923″ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedtlkt csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr aieeklanme aeirilkskl qltnklhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng lwndiscqas fkavcefpaSEQ ID NO19NP_034906 mannose binding lectin，serum(C)[Mus musculus]gi|6754656|ref|NP_034906.1|[6754656]source 1..244/organism＝″Musmusculus″/strain＝″BALB/c″/sub_species＝″domesticus″/db_xref＝″taxon:10090″/chromosome＝″19″/map＝″19 25.0 cM″/clone＝″a10″/tissue_type＝″liver″/clone_lib＝″lambda gt10″Protein 1..244/product＝″mannose binding lectin，serum(C)″sig_peptide 1..18Region 120..241/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 140..242/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS 1..244/gene＝″Mbl2″/coded_by＝″NM_010776.1:177..911″/note＝″polysaccharide-bindingcomponent of RaRF；sequence similarity to mannose-bindingproteins″/db_xref＝″LocusID:17195″/db_xref＝″MGD:96924″ORIGIN 1 msiftsflll cvvtvvyaet ltegvqnscp vvtcsspgln gfpgkdgrdg akgekgepgq61 glrglqgppg kvgptgppgn pglkgavgpk gdrgdraefd tseidseiaa lrselralrn121 wvlfslsekv gkkyfvssvk kmsldnvkal csefqgsvat prnaeensai qkvakdiayl181 gitdvrvegs fedltgnrvr ytnwndgepn ntgdgedcvv ilgngkwndv pcsdsflaic241 efsdSEQ ID NO20AAL14428 dendritic cell-specific ICAM-3 grabbing nonintegrin[Macaca nemestrina]gi|16118455|gb|AAL14428.1|AF343727_1[16118455]source 1..381/organism＝″Macaca nemestrina″/db_xref＝″taxon:9545″/cell_tvpe＝″peripheralblood-derived dend ritic cells″Protein 1..381/product＝″dendritic cell-specific ICAM-3 grabbingnonintegrin″/name＝″membrane-associated mannose binding lectin″CDS 1..381/coded_by＝″AF343727.1:1..1146″/note＝″DC-SIGN″ORIGIN 1 msdskeprlq qldlleeeql ggvgfrqtrg ykslagclgh gplvlqllsf tllagllvqv61 skvpsslsqg qskqdaiyqn ltqlkvavse lsekskqqei yqeltrlkaa vgelpekskq121 qeiyeeltrl raavgelpek sklqeiyqel trlkaavgel pekskqqeiy qelsrlkaav181 gdlpekskqq eiyqkltqlk aavdglpdrs kqqeiyqeli qlkaaverlc hpcpwewtff241 qgncyfmsns qrnwhdsita cqevgaqlvv iksaeeqnfl qlqssrsnrf twmglsdlnh301 egtwqwvdgs pllpsfkqyw nkgepnnvge edcaefsgng wnddkcnlak fwickksaas361 csgdeerlls papttpnppp aSEQ ID NO21AAF63470 mannose binding-like lectin precursor[Carassius auratus]gi|7542474|gb|AAF63470.1|AF227739_1[7542474]source 1..246/organism＝″Carassius auratus″/db_xref＝″taxon:7957″/tissue_type＝″liver″Protein＜1..246/product＝″mannose binding-like lectin precursor″/name＝″collectin″sig_peptide＜1..13Region 14..25/region_name＝″N-terminal segment″Region 26..93/region_name＝″collagen-like structure″Region 60..63/region_name＝″break in collagen structure″Region94..124/region_name＝″neck region″Region 125..246/region_name＝″carbohydrate recognition domain″/note＝″CRD″CDS 1..246/gene＝″MBL″/coded_by＝″AF227739.1:＜1..742″/note＝″collectin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose″ORIGIN 1 llllqfalql ldgaepqnln cpayggvpgt pghnglpgrd grdgkdgaig pkgekgesgv61 svqgppgkag ppgtagekge rgpsgpqgsp gsesvleslk seiqqlkaki atfekvssvc121 hfrkvgqkyy itdgvvgnfd qglkscmefg gtmvsprtsa enqallklvv ssglgskkpy181 igvtdrkteg qfvdtegkql tftnwgpgqp ddykglqdcg viedtglwdd ggcgdirpim241 ceidikSEQ ID NO22AAF63469 mannose binding-like lectin precursor[Danio rerio]gi|7542472|gb|AAF63469.1|AF227738_1[7542472]sig_peptide 1..23mat_peptide 24..251/product＝″mannose binding-like lectin″Region 24..36/region_name＝″N-terminal segment″Region 37..101/region_name＝″collagen-like structure″Region 71..74/region_name＝″break in collagen structure″Region 102..132/region_name＝″neck region″Region 133..251/region_name＝″carbohydrate recognition domain″/note＝″CRD″CDS 1..251/gene＝″mbl″/coded_by＝″AF227738.1:68..823″/note＝″collectin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose″ORIGIN 1 mallklflga llllqlvlql magaadpqsl ncpayagvpg tpghnglpgr dgrvgrdgan61 gpkgekgepg vnvqgppgka gppgpagakg ergpsglpgq dcmsdslkse lqklsdkial121 iekvvnfktf kkvgqkyyvt ddveetfdkg mqycssngga lvlprtleen allkvfvssa181 fkrlfiritd rekegefvdt drkkltftnw gpnqpdnykg aqdcgaiads glwddvscds241 lypiiceiei kSEQ ID NO23AAF63468 mannose binding-like lectin precursor[Cyprinus carpio]gi|7542470|gb|AAF63468.1|AF227737_1[7542470]sig_peptide 1..23mat_peptide 24..256/product＝″mannose binding-like lectin″Region 24..35/region_name＝″N-terminal segment″Region 36..103/region_name＝″collagen-like structure″Region 70..73/region_name＝″break in collagen structure″Region 104..134/region_name＝″neck region″Region 135..256/region_name＝″carbohydrate recognition domain″/note＝″CRD″CDS 1..256/gene＝″MBL″/coded_by＝″AF227737.1:67..837″/note＝″collsctin with structuralhomology to mannose-binding lectin but with a predicted carbohydrate specificity forgalactose″ORIGIN 1 malfklflgt llllqfalql ldgaepqnln cpayggvpgt pghnglpgrd grdgkdgaig61 pkgekgesgv svqgppgkag ppgpagekge rgptgsqgsp gsesvleslk seiqqlkaki121 atfekvasvg hfrqvgqkyy itdgvvgtfd qglkfckdfg gtmvfprtsa enqallklvv181 ssglsskkpy igvtdreteg rfvntegkql tftnwgpgqp ddykglqdcg viedsglwdd241 gscgdirpim ceidnkSEQ ID NO24AAF21018 mannose-binding lectin 2[Sus scrofa]gi|6644342|gb|AAF21018.1AF208528_1[6644342]source 1..31/organism＝″Susscrofa″/db_xref＝″taxon:9823″/chromosome＝″14″/map＝″between S0007 and Sw210″Protein<1..>31/product＝″mannose-binding lectin 2″/name＝″MBL2″CDS 1..31/gene＝″MBL2″/coded_by＝″join(AF208528.1:<..25，AF208528.1:703..>771)″ORIGIN 1 tkgekgepgp gfrgsqgppg kmgppgnige tSEQ ID NO25AAK30298 mannose-binding lectin precursor protein[Gallus gallus]gi|13561409|gb|AAK30298.1|[13561409]sig_peptide 1..21mat_peptide 22..254/product＝″mannose-binding lectin protein″Region 22..46/region name＝″N-terminal segment″Region 47..102/region_name＝″collagen-like″Region 66/region_name＝″break in collagen-like structure″Region 103..139/region_name＝″neck region″Region 140..254/region_name＝″carbohydrate recognition domain；CRD″CDS 1..254/coded_by＝″AF231714.1:242..1006″ORIGIN 1 mtllqpfsal llclslmmat sllttdkpee kmyscpiiqc sapavnglpg rdgrdgpkge61 kgdpgeglrg lqglpgkagp qglkgevgpq gekgqkgerg ivvtddlhrq itdleakirv121 leddlsrykk alslkdvvnv gkkmfvstgk kynfekgksl cakagsvlas prneaental181 kdlidpssqa yigisdaqte grfmylsggp ltysnwkpge pnnhknedca viedsgkwnd241 ldcsnsnifi icelSEQ ID NO26LNMSMC mannose-bin ding lectin C precursor-mouse gi|7428747|pir||LNMSMC[7428747]FEATURES Location/Qualifiers source 1..244/organism＝″Musmusculus″/db_xref＝″taxon:10090″Protein 1..244/product＝″mannose-binding lectin C precursor″/note＝″Ra-reactive factor P28a″Region 1..18/region_name＝″domain″/note＝″signal sequence″Region 19..244/region_name＝″product″/note＝″mannose-binding lectin C″Bond bond(29)/bond_type＝″disulfide″/note＝″interchain″Bond bond(34)/bond_type＝″disulfide″/note＝″interchain″Region 38..94/region_name＝″region″/note＝″collagen-lie″Site 69/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Region 124..240/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 msiftsflll cvvtvvyaet ltegvqnscp vvtcsspgln gfpgkdgrdg akgekgepgq61 glrglqgppg kvgptgppgn pglkgavgpk gdrgdraefd tseidseiaa lrselralrn121 wvlfslsekv gkkyfvssvk kmsldrvkal csefqgsvat prnaeensai qkvakdiayl181 gitdvrvegs fedltgnrvr ytnwndgepn ntgdgedcvv ilgngkwndv pcsdsflaic241 efsdSEQ ID NO27LNMSMA mannose-binding lectin A precursor-mouse gi|625320|pir||LNMSMA[625320]FEATURES Location/Qualifiers source 1..23g/organism＝″Musmusculus″/db_xref＝″taxon:10090″Protein 1..239/product＝″mannose-binding lectin A precursor″/note＝″Ra-reactive factor P28b；serum mannan-binding protein″Region 1..17/region_name＝″domain″/note＝″signal sequence″Region 18..238/region_name＝″product″/note＝″mannose-binding lectin A″Region 36..88/region_name＝″region″/note＝″collagen-lie″Region 119..235/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 mlllpllpvl lcvvsvsssg sqtcedtlkt csviacgrdg rdgpkgekge pgqglrglqg61 ppgklgppgs vgspgspgpk gqkgdhgdnr aieeklanme aeirilkskl qltnklhafs121 mgkksgkklf vtnhekmpfs kvkslctelq gtvaiprnae enkaiqevat giaflgitde181 ategqfmyvt ggrltysnwk kdepnnhgsg edcviildng lwndiscqas fkavcefpaSEQ ID NO28LNRTMA mannose-binding lectin A precursor-rat gi|71975|pir||LNRTMA[71975]FEATURES Location/Qualifiers source 1..238/organism＝″Rattusnorvegicus″/db_xref＝″taxon:10116″Protein 1..238/product＝″mannose-binding lectin A precursor″/note＝″serum mannan-bindingprotein″Region 1..17/region_name＝″domain″/note＝″signal sequence″Region 18..238/region_name＝″product″/note＝″mannose-binding lectin A”Region 36..88/region_name＝″region″/note＝″collagen-like″Site 61/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 67/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 73/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Site 79/site_type＝″modified″/note＝″lysine derivativa(Lys)(probably 5-hydroxylysine)″Site 82/site_type＝″modified″/note＝″lysine derivative(Lys)(probably 5-hydroxylysine)″Region 85..87/region_name＝″region″/note＝″cell attachment(R-G-D)motif″Region 118..234/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 mlllpllvll cvvsvsssgs qtceetlktc sviacgrdgr dgpkgekgep gqglrglqgp61 pgklgppgsv gapgsqgpkg qkgdrgdsra ievklanmea eintlkskle ltnklhafsm121 gkksgkkffv tnhermpfsk vkalcselrg tvaiprnaee nkaiqevakt saflgitdev181 tegqfmyvtg grltysnwkk depndhgsge dcvtivdngl wndiscqash tavcefpaSEQ ID NO29LNRTMC mannose-binding lectin C precursor-rat gi|71974|pir||LNRTMC[71974]FEATURES Location/Qualifiers source 1..244/organism＝″Rattusnorvegicus″/db_xref＝″taxon:10116″Protein 1..244/product＝″mannose-binding lectin C precursor″Region 1..18/region_name＝″domain″/note＝″signal sequence″Region 19..244/region_name＝″product″/note＝″mannose-binding lectin C″Bond bond(29)/bond_type＝″disulfide″/note＝″interchain″Bond bond(34)/bond_type＝″disulfide″/note＝″interchain″Region 38..94/region_name＝″region″/note＝″collagen-like″Site 69/site_type＝″modified″/note＝″4-hydroxyproline(Pro)″Region 124..240/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 mslftsflll cvltavyaet ltegaqsscp viacsspgln gfpgkdghdg akgekgepgq61 glrglqgppg kvgpagppgn pgskgatgpk gdrgesvefd ttnidleiaa lrselramrk121 wvllsmsenv gkkyfmssvr rmplnrakal cselqgtvat prnaeenrai qnvakdvafl181 gitdqrtenv fedltgnrvr ytnwnegepn nvgsgencvv lltngkwndv pcsdsflvvc241 efsdSEQ ID NO30LNHUMC mannose-binding lectin precursor-human gi|71973|pir||LNHUMC[71973]FEATURES Location/Qualifiers source 1..248/organism＝″Homosapjens″/db_xref＝″taxon:9606″Protein 1..248/product＝″mannose-binding lectin precursor″/note＝″mannan-binding protein″Region 1..20/region_name＝″domain″/note＝″signal sequence″Region 21..248/region_ame＝″product″/note＝″mannose-binding lectin″Region 42..99/region_name＝″region″/note＝″collagen-like″Site 47/site_type＝″modified″/note＝″4-hydroxyproline(Pro)(partial)″Site 73/site_type＝″modified″/note＝″4-hydroxyproline(Pro)(partial)″Site 79/site_type＝″modified″/note＝″4-hydroxyproline(Pro)(partial)″Site 82/site_type＝″modified″/note＝″4-hydroxyproline(Pro)(partial)″Site 88/site_type＝″modified″/note＝″4-hydroxyproline(Pro)(partial)″Region 128..244/region_name＝″domain″/note＝″C-type lectin homology#label LCH″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO31BAA86864 complement C1s[Homo sapiens]gi|6407558|dbj|BAA86864.1|[6407558]FEATURES Location/Qualifiers source 1..329/organism＝″Homosapiens″/db_xref＝″taxon:9606″/tissue_type＝″peripheral leukocytes″/clone_lib＝″FIXII″Protein 1..329/product＝″complement C1s″CDS 1..329/coded_by＝″join(AB009076.1:1142..1146，AB009076.1:1703..1910，AB009076.1:2118..2295，AB009076.1:3495..3620，AB009076.1:4328..4527，AB009076.1:5047.5200，AB009076.1:5748..＞5863)″/note＝″This gene consists of total 12exons，the last 4 exons of which were reported by Toshi，M.et al.(J.Mol.Biol.208:709-714，1989)ORIGIN 1 mwcivlfsll awvyaeptmy geilspnypq aypseveksw dievpegygi hlyfthldie61 lsencaydsv qijsgdteeg ricgqrssnn phspiveefq vpynklqvif ksdfsneerf121 tgfaayyvat dinectdfvd vpcshfcnnf iggyfcscpp eyflhddmkn cgvncsgdvf181 taligeiasp nypkpypens rceyqirlek gfqvvvtlrr edfdveaads agncldslvf241 vagdrqfgpy cghgfpgpln ietksnaldi ifqtdltgqk kgwklryhgd pmpcpkedtp301 nsvwepakak yvfrdvvqit cldgfevveSEQ ID NO32CAB56124 mannose-binding lectin[Homo sapiens]gi|5911809|emb|CAB56124.1|[5911809]FEATURES Location/Qualifiers source 1..248/organism＝ ″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype HYPD″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16582.1:892..1638″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd gcdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO33CAB56123 mannose-binding lectin[Homo sapiens]gi|5911807|emb|CAB56123.1|[5911807]FEATURES Location/Qualifiers source 1..248/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype HYPA″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16581.1:892..1638″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO34CAB56122 mannose-binding lectin[Homo sapiens]gi|5911798|emb|CAB56122.1|[5911798]FEATURES Location/Qualifiers source 1..248/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype LXPA″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16580.1:892..1638″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO35CAB56121 mannose-binding lectin[Homo sapiens]gi|5911796|emb|CAB56121.1|[15911796]FEATURES Location/Qualifiers source 1..248/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype LYPB″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16579.1:892..1638″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grddtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO36CAB56045 mannose-binding lectin[Homo sapiens]gi|5911794|emb|CAB56045.1|[5911794]/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype LYQC″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16578.1:886..1632″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkeekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO37CAB56120 mannose-binding lectin[Homo sapiens]gi|5911792|emb|CAB56120.1|[5911792]FEATURES Location/Qualifiers source 1..248/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype LYPA″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16577.1:892..1638″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO38CAB56044 mannose-binding lectin[Homo sapiens]gi|5911790|emb|CAB56044.1|[5911790]FEATU RES Location/Qualifiers source 1..248/organism＝″Homosapiens″/db_xref＝″taxon:9606″/chromosome＝″10″/map＝″10q11.2-q21″/note＝″MBLhaplotype LYQA″Protein 1..248/product＝″mannose-binding lectin″sig_peptide 1..20CDS 1..248/gene＝″MBL″/coded_by＝″Y16576.1:886..1632″ORIGIN 1 mslfpslpll llsmvaasys etvtcedaqk tcpaviacss pgingfpgkd grdgtkgekg61 epgqglrglq gppgklgppg npgpsgspgp kgqkgdpgks pdgdsslaas erkalqtema121 rikkwltfsl gkqvgnkffl tngeimtfek vkalcvkfqa svatprnaae ngaiqnlike181 eaflgitdek tegqfvdltg nrltytnwne gepnnagsde dcvlllkngq wndvpcstsh241 lavcefpiSEQ ID NO39AAB53110 C1qR(p)[Homo sapiens]gi|2052498|gb|AAB53110.1|[2052498]FEATURES Location/Qualifiers source 1..652/organism＝″Homosapiens″/db_xref＝″taxon:9606″/cell_line＝″U937 histiocytic cell line″Protein 1..652/product＝″C1qR(p)″/function＝″mediates enhanced phagocytosis by humanmonocytes and macrophages in response to complement C1q，mannose binding lectin(MBL)and pulmonary surfactant protein A(SPA)″CDS 1..652/coded_by＝″U94333.1:149..2107″/note＝″Clq/MBL/SPA receptor″ORIGIN 1 matsmgllll llllltqpga gtgadteavv cvgtacytah sgklsaaeaq nhcnqnggnl61 atvkskeeaq hvqrvlaqll rreaaltarm skfwiglqre kgkcldpslp lkgfswvggg121 edtpysnwhk elrnsciskr cvsllldlsq pllpnrlpkw segpcgspgs pgsniegfvc181 kfsfkgmcrp lalggpgqvt yttpfqttss sleavpfasa anvacgegdk detqshyflc241 kekapdvfdw gssgplcvsp kygcnfnngg chqdcfeggd gsflcgcrpg frllddlvtc301 asrnpcsssp crggatcvlg phgknytcrc Pqgyqldssq ldcvdvdecq dspcaqecvn361 tpggfrcecw vgyepggpge gacqdvdeca lgrspcaqgc tntdgsfhcs ceegyvlage421 dgtqcqdvde cvgpggplcd slcfntqgsf hcgclpgwvl apngvsctmg pvslgppsgp481 pdeedkgeke gstvpraata sptrgpegtp katpttsrps lssdapitsa plkmlapsgs541 sgvwrepsih hataasgpqe paggdssvat qnndgtdgqk lllfyilgtv vaillllala601 lgllvyrkrr akreekkekk pqnaadsysw vperaesram enqysptpgt dcSEQ ID NO40NP_571645 mannose binding-like lectin[Danio rerio]gi|18858997|ref|NP_571645.1|[18858997]sig_peptide 1..23mat_peptide 24..251/product＝″mannose binding-like lectin″Region 24..36/region_name＝″N-terminal segment″Region 33..70/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″Region 33..70/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″Region 37..101/region_name＝″collagen-like structure″Region 37..70/region_name＝″Collagen triple helix repeat(20copies)″/note＝″Collagen″/db_xref＝″CDD:pfam01391″Region 71..74/region_name＝″break in collagen structure″Region 102..132/region_name＝″neck region″Region 133..251/region_name＝″carbohydrate recognition domain″/note＝″CRD″Region 134..247/region_name＝″C-type lectin(CTL)or carbohydrate-recognition domain(CRD)″/note＝″CLECT″/db_xref＝″CDD:smart00034″Region 146..247/region_name＝″Lectin C-typedomain″/note＝″lectin_c″/db_xref＝″CDD:pfam00059″CDS 1..251/gene＝″mbl″/coded_by＝″NM_131570.1:68..823″/note＝″collectin with structuralhomology to mannose-pinding lectin but with a predicted carbohydrate specificity forgalactose；mannose binding-like lectin″/db_xref＝″LocusID:58091″ORIGIN 1 mallklflga llllqlvlql magaadpqsl ncpayagvpg tpghnglpgr dgrvgrdgan61 gpkgekgepg vnvqgppgka gppgpagakg ergpsglpgq dcmsdslkse lqklsdkial121 iekvvnfktf kkvgqkyyvt ddveetfdkg mqycssngga lvlprtleen allkvfvssa181 fkrlfiritd rekegefvdt drkkltftnw gpnqpdnykg aqdcgaiads glwddvscds241 lypiiceiei kSEQ ID NO41BAA90338 mannose-binding lectin-associated serine protease(MASP)related protein[Cyprinus carpio]gi|6807499|dbj|BAA90338.1|[6807499]FEATURES Location/Qualifiers source 1..118/organism＝″Cyprinuscarpio″/db_xref＝″taxon:7962″Protein 1..118/product＝″mannose-binding lectin-associated serine protease(MASP)relatedprotein″CDS 1..118/gene＝″MRPb″/coded_by＝″join(AB030447.1:＜1.96，AB030447.1:201..319，AB030447.1:436..514，AB030447.1:616..680)″/note＝″MASP-related protein″ORIGIN 1 kiqtgsntvs ilfhsdnsgd nlgwkltyts tgsecsplaa plnghleplq snyifkdhim61 ltcdpgyslr qgdkefehyq iecqrdgkws sdvplckkke sqrrhrslps iltnqils所述第二多肽优选包含所述胶原凝集素或所述蛋白的变体或同系物的至少10个，如至少12个，例如至少15个，如至少20个，例如至少25个，如至少30个，例如至少35个，如至少40个，例如至少50个连续的氨基酸残基。此类变体或同系物优选与所述胶原凝集素至少70％，如80％，例如90％，如95％相同。
优选实施方案中，所述第二多肽序列包含MBL的CRD区或MBL的颈区或MBL的胶原蛋白样区。更优选所述第二多肽包含MBL的颈区和CRD区。在更优选的实施方案中，所述第二多肽序列包含MBL的胶原蛋白样区，颈区和CRD区。MBL如上所限。
优选所述第二多肽序列包含至少为图2所示MBL序列的氨基酸170-200，如至少为图2所示MBL序列的氨基酸160-200，如至少为图2所示MBL序列的氨基酸150-200，如至少为图2所示MBL序列的氨基酸140-200，如至少为图2所示MBL序列的氨基酸130-200，如至少为图2所示MBL序列的氨基酸120-200，如至少为图2所示MBL序列的氨基酸110-200，如至少为图2所示MBL序列的氨基酸100-200，如至少为图2所示MBL序列的氨基酸90-200，如至少为图2所示MBL序列的氨基酸80-200，如至少为图2所示MBL序列的氨基酸70-200，如至少为图2所示MBL序列的氨基酸60-200，如至少为图2所示MBL序列的氨基酸80-228。
优选所述第二多肽序列包含SEQ ID.NO 2的氨基酸80-228。
优选实施方案中，所述第二多肽序列能连接至少一个MASP蛋白，如选自MASP-1，MASP-2和MASP-3的MASP蛋白或其功能同系物或变体。特别是当所述第二多肽是融合蛋白的一部分时，它能够连接所述至少一个MASP蛋白。因此，所述第二多肽序列能够给所述融合蛋白提供激活补体系统的活性。优选实施方案中，所述第二多肽序列包含选自如下的氨基酸序列SEQ ID.NO 2的56-228，SEQ ID.NO 2的55-228，SEQ ID.NO 2的54-228，和SEQ ID.NO 2的50-228。优选实施方案中，所述第二多肽序列具有选自如下的氨基酸序列SEQ ID.NO 2的56-228，SEQ ID.NO 2的55-228，SEQID.NO 2的54-228，和SEQ ID.NO 2的50-228。
另一实施方案中，所述第二多肽包含所述胶原凝集素的半胱氨酸富集区，如所述胶原凝集素的N-末端区。
融合蛋白所述融合蛋白包含相互连接的第一和第二多肽，可选通过接头区连接。优选实施方案中，所述第一多肽序列位于所述融合蛋白的N-末端并且所述第二多肽序列位于C-末端。
所述融合蛋白组成的具体实例是-融合蛋白，其包含L-ficolin的半胱氨酸富集区和胶原蛋白样区以及MBL的CRD区。
-融合蛋白，其包含L-ficolin的半胱氨酸富集区以及MBL的胶原蛋白样区，颈区和CRD区。
-融合蛋白，其包含H-ficolin的半胱氨酸富集区和胶原蛋白样区以及MBL的CRD区。
-融合蛋白，其包含H-ficolin的半胱氨酸富集区以及MBL的胶原蛋白样区，颈区和CRD区。
-融合蛋白，其包含M-ficolin的半胱氨酸富集区和胶原蛋白样区以及MBL的CRD区。
-融合蛋白，其包含M-ficolin的半胱氨酸富集区以及MBL的胶原蛋白样区，颈区和CRD区。
-融合蛋白，其包含MBL的半胱氨酸富集区，以及ficolin的CRD区。
-融合蛋白，其包含MBL的半胱氨酸富集区以及ficolin的胶原蛋白样区，颈区和CRD区。
-融合蛋白，其包含L-ficolin的半胱氨酸富集区和胶原蛋白样区以及肺表面活性相关性-蛋白D的CRD区。
-融合蛋白，其包含L-ficolin的半胱氨酸富集区以及肺表面活性相关性-蛋白D的胶原蛋白样区，颈区和CRD区。
-融合蛋白，其包含ficolin的半胱氨酸富集区和胶原蛋白样区以及胶原凝集素-43的CRD区。
-融合蛋白，其包含ficolin的半胱氨酸富集区以及胶原凝集素-43的胶原蛋白样区，颈区和CRD区。
包含图3所示氨基酸序列的融合蛋白或其功能同系物，优选由图3所示氨基酸序列组成的融合蛋白。另一个实施方案中，所述融合蛋白具有图1所示氨基酸1-50的氨基酸序列和图2所示氨基酸54-228的氨基酸序列。
如上所讨论，所述融合蛋白优选能够形成亚基复合体和亚基复合体的寡聚体。优选所述融合蛋白基本只形成三聚的，四聚的，五聚的和六聚的亚基寡聚体，如三聚的，四聚的，和五聚的亚基寡聚体，如三聚的或四聚的亚基寡聚体，更优选基本只有四聚的亚基寡聚体，以便使融合蛋白的组成更均一。
同系物本说明书中，术语同系物或变体或功能同系物是同义的，其中蛋白的同系物表现出一或更多氨基酸残基的一或多个取代，缺失，和/或添加。片段是一个亚组的同系物，其为所述蛋白的截短物(truncation)。
所述蛋白的同系物可包含一或更多个保守氨基酸取代，如至少2个保守氨基酸取代，例如至少3个保守氨基酸取代，如至少5个保守氨基酸取代，例如至少10个保守氨基酸取代，如至少20个保守氨基酸取代，例如至少50个保守氨基酸取代如至少75个保守氨基酸取代，例如至少100个保守氨基酸取代。本发明含义中的保守氨基酸取代是指将一组预定(predetermined)氨基酸中的一个氨基酸取代为相同预定组中表现出类似或基本类似性质的另一氨基酸。所述预定组例如极性侧链(Asp，Glu，Lys，Arg，His，Asn，Gln，Ser，Thr，Tyr和Cys，)非极性侧链(Gly，Ala，Val，Leu，Ile，Phe，Trp，Pro和Met)脂肪族侧链(Gly，Ala Val，Leu，Ile)环状侧链(Phe，Tyr，Trp，His，Pro)芳香族侧链(Phe，Tyr，Trp)酸性侧链(Asp，Glu)碱性侧链(Lys，Arg，His)酰胺侧链(Asn，Gln)羟基侧链(Ser，Thr)含硫侧链(Cys，Met)，和为单氨基-二羧酸或单氨基-单羧基-单酰胺羧酸(monoamino-monocarboxylic-monoamidocarboxylic acid)的氨基酸(Asp，Glu，Asn，Gln)。
可在优选蛋白的任何位置引入保守取代。然而，可能也希望引入非-保守取代。非-保守取代应导致形成能够发挥(exert)与所述蛋白类似功能的蛋白同系物。此类取代可例如i)疏水性截然不同，例如疏水残基(Val，Ile，Leu，Phe或Met)被亲水残基如Arg，Lys，Trp或Asn取代，或亲水残基如Thr，Ser，His，Gln，Asn，Lys，Asp，Glu或Trp被疏水残基取代；和/或ii)对多肽骨架取向(orientation)的影响截然不同，如用其他残基取代Pro或Gly或使其他残基被Pro或Gly取代；和/或iii)电荷截然不同，例如带负电荷的残基如Glu或Asp被带正电荷的残基如Lys，His或Arg取代(和反之)；和/或iv)空间占位(stericbulk)截然不同，例如占位大的(bulky)残基如His，Trp，Phe或Tyr被侧链较小的残基，例如Ala，Gly或Ser取代(和反之)。
本发明另一个实施方案涉及优选蛋白的同系物，其中所述同系物包含取代的氨基酸，其具有的亲水或亲疏水(hydropathic)指数在它所取代的氨基酸的值的+/-2.5以内，例如+/-2.3以内，如+/-2.1以内，例如+/-2.0以内，如+/-1.8以内，例如+/-1.6以内，如+/-1.5以内，例如+/-1.4以内，如+/-1.3以内，例如+/-1.2以内，如+/-1.1以内，例如+/-1.0以内，如+/-0.9以内，例如+/-0.8以内，如+/-0.7以内，例如+/-0.6以内，如+/-0.5以内，例如+/-0.4以内，如+/-0.3以内，例如+/-0.25以内，如+/-0.2以内。
所述亲水和亲疏水的氨基酸指数对于赋予蛋白相互作用生物功能的重要性在本领域已知(Kyte & Doolittle，1982和Hopp，美国专利4,554,101，每篇引入本文作为参考)。
本文所用的氨基酸亲疏水性指数值是异亮氨酸(+4.5)；缬氨酸(+4.2)；亮氨酸(+3.8)；苯丙氨酸(+2.8)；半胱氨酸/胱氨酸(+2.5)；蛋氨酸(+1.9)；丙氨酸(+1.8)；甘氨酸(-0.4)；苏氨酸(-0.7)；丝氨酸(-0.8)；色氨酸(-0.9)；酪氨酸(-1.3)；脯氨酸(-1.6)；组氨酸(-3.2)；谷氨酸(-3.5)；谷氨酰胺(-3.5)；天冬氨酸(-3.5)；天冬酰胺(-3.5)；赖氨酸(-3.9)；和精氨酸(-4.5)(Kyte & Doolittle，1982)。
所述氨基酸亲水性值为精氨酸(+3.0)；赖氨酸(+3.0)；天冬氨酸(+3.0.+-.1)；谷氨酸(+3.0.+-.1)；丝氨酸(+0.3)；天冬酰胺(+0.2)；谷氨酰胺(+0.2)；甘氨酸(0)；苏氨酸(-0.4)；脯氨酸(-0.5.+-.1)；丙氨酸(-0.5)；组氨酸(-0.5)；半胱氨酸(-1.0)；蛋氨酸(-1.3)；缬氨酸(-1.5)；亮氨酸(-1.8)；异亮氨酸(-1.8)；酪氨酸(-2.3)；苯丙氨酸(-2.5)；色氨酸(-3.4)(U.S.4,554,101)。
因此，一个实施方案中的氨基酸取代可以以它们的疏水性和亲水性值和氨基酸侧链取代基，包括电荷，大小等等的相对类似性为基础。考虑到多种前述性质的举例氨基酸取代对本领域技术人员是公知的，其包括精氨酸和赖氨酸；谷氨酸和天冬氨酸；丝氨酸和苏氨酸；谷氨酰胺和天冬酰胺；以及缬氨酸、亮氨酸和异亮氨酸。
另外，同系物可包含氨基酸的添加或缺失，例如添加或缺失2-100个氨基酸，如2-50个氨基酸，例如2-20个氨基酸，如2-10个氨基酸，例如2-5个氨基酸，如2-3个氨基酸。然而，添加超过100个氨基酸，如添加100-500个氨基酸也包括在本发明中。
与优选蛋白至少有一些同源性的蛋白，当它们与所述优选蛋白至少约40％同源或优选相同，例如与所述优选蛋白例如至少约50％同源或优选相同，例如至少约60％同源或优选相同，如至少约70％同源或优选相同，例如至少约75％同源或优选相同，如至少约80％同源或优选相同，例如至少约85％同源或优选相同，如至少约90％同源或优选相同，例如至少92％同源或优选相同，如至少94％同源或优选相同，例如至少95％同源或优选相同，如至少96％同源或优选相同，例如至少97％同源或优选相同，如至少98％同源或优选相同，例如至少99％同源或优选相同时落入本发明范围。
优选蛋白是包含胶原凝集素和凝集素及其同系物的补体激活蛋白。
胶原凝集素同系物本发明范围中，胶原凝集素包括MBL的同系物被理解为任何能够发挥与胶原凝集素功能类似的功能并包含一或多个上述变异的蛋白。特别是，所述功能是通过结合一或多个糖来激活补体的能力。
本文所用术语“胶原凝集素的功能同系物”涉及包含预确定的氨基酸序列的胶原凝集素功能等效物(equivalent)或片段，并且所述同系物被限定为a)包含能单独或如上述发生亚基复合后，识别并结合葡聚糖，脂磷酸聚糖和糖肌醇磷脂(glycoinositol phospholipid)的氨基酸序列的同系物，所述葡聚糖，脂磷酸聚糖和糖肌醇磷脂包含吡喃糖环中有3-和4-羟基基团的糖(即Man，Glc，Fuc或GlcNAc)，和/或b)包含能单独或当如上述发生亚基复合后，与凝集素/MBL途径的组分建立连接，如结合MASP-1，MASP-2，MASP-3和/或sMAP的氨基酸序列的同系物，所述结合导致激活凝集素/MBL途径，和/或c)包含能通过胶原蛋白样区形成两或多个亚基的寡聚体结构的氨基酸序列的同系物，其中所述亚基包含具有半胱氨酸富集区、胶原蛋白样区、颈区和糖识别区的三个相同多肽。
凝集素同系物本发明范围中，凝集素包括ficolin的同系物被理解为任何能够发挥与凝集素功能类似的功能并包含一或多个上述变异的蛋白。特别是，所述功能是通过结合一或更多个糖来激活补体的能力。
本文所用术语“凝集素的功能同系物”涉及包含预确定的氨基酸序列的凝集素功能等效物或片段，并且所述同系物被限定为
a)包含单独或如上述发生亚基复合后，能够识别并结合N-乙酰葡萄糖胺(GlcNAc)，或N-乙酰半乳糖胺(GalNAc)或弹性蛋白的氨基酸序列的同系物，和/或b)包含单独或如上述发生亚基复合后，能够与凝集素/MBL途径的组分建立连接，如结合MASP-1，MASP-2，MASP-3和/或sMAP的氨基酸序列的同系物，所述结合导致激活凝集素/MBL途径，和/或c)包含能够通过胶原蛋白样区形成两或多个亚基的寡聚体结构的氨基酸序列的同系物，其中所述亚基包含具有半胱氨酸富集区、胶原蛋白样区、颈区和纤维蛋白原样区的三个相同多肽。
凝集素/MBL途径的激活，即融合蛋白激活补体系统的活性，可通过评估所述融合蛋白或亚基复合体或其寡聚复合体的C4裂解效应来评估，后者可以通过包括如下步骤的方法来评估-应用(applying)包含预确定量的融合蛋白以及预确定量的MASP-1，MASP-2或MASP-3的样品，-将至少一种补体因子应用到所述样本，-检测被裂解的补体因子的量，-使所述被裂解的补体因子量与融合蛋白的量相关，并-确定所述融合蛋白的活性。
优选用于本方法的补体因子是被MBL/MASP-2复合体裂解的补体因子，如C4。然而，所述补体因子也可选自C3和C5。
所述被裂解的补体因子可用多种手段(means)检测，如用针对所述被裂解的补体因子的抗体检测。
在使经典补体激活途径和替代补体激活途径的干扰最小化或去除的条件下进行试验。
优选胶原凝集素和/或凝集素的同系物显示上述功能中的两种，更优选上述功能中的三种。
制备融合蛋白所述融合蛋白可用任何本领域技术人员公知的合适方法制备。如下描述数种制备所述融合蛋白的方法，然而本发明不限于所述方法。
合成制备合适时，特别是涉及融合蛋白的大小时，所述融合蛋白可通过合成来制备。合成制备肽的方法本领域公知。制备合成肽的具体描述以及实用的建议可见Synthetic PeptidesA User′s Guide(Advances in Molecular Biology)，GrantG.A.ed.，Oxford University Press，2002或者Pharmaceutical FormulationDevelopment of Peptides and Proteins，Frokjaer and Hovgaard eds.，Taylor andFrancis，1999。
重组制备本发明融合蛋白优选用重组DNA技术制备。制备编码融合蛋白每一部分的DNA序列可用DNA酶I根据标准方案(Sambrook等，Molecular cloningA Laboratory manual.2 rd ed.，CSHL Press，Cold Spring Harbor，NY，1989)将衍生所述融合蛋白部分的全长蛋白的DNA编码序列(基因组DNA或cDNA)裂解。然后将所得的编码所述融合蛋白各个部分的DNA序列融合到一起。
所述DNA序列也可以通过聚合酶链式反应用特异性引物来制备，例如美国专利4,683,202或Saiki等，1988，Science 239487-491所述。
编码本发明融合蛋白的DNA序列可以用已有的标准方法通过合成来制备，例如Beaucage和Caruthers，1981，Tetrahedron Lett.221859-1869描述的亚膦酰胺(phosphoamidine)方法，或Matthes等，1984，EMBO J.3801-805描述的方法。根据亚膦酰胺方法，在例如自动DNA合成仪中合成寡核苷酸，纯化、退火、连接并克隆到合适的载体中。
然后将所述DNA序列插入重组表达载体，其可以是任何可方便地进行重组DNA操作的载体。选择载体通常依赖于它要被引入的宿主细胞。因此，所述载体可以是自主复制载体，即作为染色体外实体(entity)存在、且其复制独立于染色体复制的载体，例如质粒。或者，所述载体可以在引入宿主细胞时，被整合到宿主细胞基因组，并与它所整合的染色体一起复制。
载体中，编码融合蛋白的DNA序列应被可操作性连接到合适的启动子序列。所述启动子可以是在所选择的宿主细胞中显示转录活性的任何DNA序列，可来自与所述宿主细胞同源或不同源的蛋白的编码基因。指导编码DNA序列在哺乳动物细胞内转录的合适启动子举例有SV40启动子(Subramani等，1981，Mol.Cell Biol.1854-864)，MT-1(金属硫蛋白基因)启动子(Palmiter等，1983，Science 222809-814)或腺病毒2主要晚期启动子。合适用于昆虫细胞的启动子是多角体(polyhedrin)启动子(Vasuvedan等，1992，FEBS Lett.3117-11)。合适用于酵母宿主细胞的启动子包括来自酵母糖酵解基因(Hitzeman等，1980，J.Biol.Chem.25512073-12080；Alber和Kawasaki，1982，J.Mol.Appl.Gen.1419-434)或醇脱氢酶基因(Young等，1982，inGenetic Engineering of Microorganisms for Chemicals，Hollaender等eds.，Plenum Press，New York)，或TPI1(美国专利4,599,311)或ADH2-4c(Russell等，1983，Nature 304652-654)的启动子。合适用于丝状真菌宿主细胞的启动子是，例如，ADH3启动子(McKnight等，1985，EMBO J.42093-2099)或tpiA启动子。
所述DNA编码序列也可以可操作地与合适的终止子，如人生长激素终止子(Palmiter等，如前引用)或(用于真菌宿主时)TPI1(Alber和Kawasaki，如前引用)或ADH3(McKnight等，如前引用)启动子连接。该载体还可包含聚腺苷酸化信号(例如来自SV 40或腺病毒5 Elb区)，转录增强子序列(例如SV 40增强子)和翻译增强子序列(例如编码腺病毒VARNA的序列)等元件。
所述重组表达载体还可包含使所述载体在目标宿主细胞中复制的DNA序列。此种序列举例(当所述宿主细胞是哺乳动物细胞时)是SV40复制起点。所述载体也可包含选择性标记物，例如其产物弥补了宿主细胞的缺陷的基因，如编码二氢叶酸还原酶(DHFR)的基因或带有(confer)对药物，例如新霉素，潮霉素或氨甲蝶呤的抗性的基因。
本领域技术人员公知用于连接分别编码所述融合蛋白、启动子和终止子的DNA序列并将它们插入合适的包含复制必要信息的载体的方法(参见(cf.)，例如，Sambrook等，如前引用)。
合成所述重组融合蛋白可使用体外或体内培养物。宿主细胞培养物优选是真核宿主细胞培养物。本文中真核细胞培养物转化是指将重组DNA引入所述细胞。此方法中用的表达构建体的特征为具有选自哺乳动物基因的编码区，所述哺乳动物基因包括人基因和与非常相似的基因如黑猩猩(chimpanzee)的基因。所用的表达构建体还具有的特征为，启动子区选自病毒或真核生物，包括哺乳动物细胞和昆虫细胞的基因。
根据本发明制备重组MBL的方法其特征在于，所述宿主细胞培养物优选是真核生物的，例如是哺乳动物的细胞培养物。优选的宿主细胞培养物是人肾细胞的培养物，更优选，宿主细胞培养物是人胚胎肾细胞(HEK细胞)的培养物，如用于制备重组人MBL的HEK 293细胞系。“HEK 293细胞系”指任何源于人胚胎肾组织的细胞系，例如但不限于在美国典型培养物保藏中心(American Type Culture Collection)以号码CRL-1573和CRL-10852保藏的细胞系。
其他细胞可以是鸡胚胎成纤维细胞，仓鼠卵巢细胞，幼仓鼠(baby hamster)肾细胞，人宫颈癌细胞(cervical carcinoma cell)，人黑素瘤细胞，人肾细胞，人脐血管(nmbilical vascular)内皮细胞，人脑内皮细胞，人口腔肿瘤细胞，猴肾细胞，小鼠成纤维细胞，小鼠肾细胞，小鼠结缔组织细胞，小鼠寡分支(oligodendritic)细胞，小鼠吞噬细胞，小鼠成纤维细胞，小鼠成神经细胞瘤细胞，小鼠前B细胞，小鼠B淋巴细胞瘤细胞，小鼠浆细胞瘤细胞，小鼠恶性畸胎瘤细胞，大鼠星形细胞瘤细胞，大鼠乳房(mammary)上皮细胞，COS，CHO，BHK，VERO，HeLa，MDCK，W138和NIH 3T3细胞。
或者，真菌细胞(包括酵母细胞)可用作宿主细胞。合适的酵母细胞举例包括糖酵母(Saccharomyces spp.)或裂殖酵母(Schizosaccharomyces spp.)的细胞，特别是酿酒酵母(Saccharomyces cerevisiae)的细胞株。其他真菌细胞举例是丝状真菌，例如曲霉(Aspergillus spp.)或链孢霉(Neurospora spp)，特别是米曲霉(Aspergillus oryzae)或黑曲霉(Aspergillus niger)株。曲霉(Aspergillus spp.)用于蛋白表达的用途见例如EP 238 023描述。
另外，可选择那些调控插入序列的表达、或按所需特定方式修饰并加工基因产物的宿主细胞株。蛋白产物的这类修饰(例如，糖基化)和加工(例如，裂解)可能对于所述蛋白的功能是重要的。不同的宿主细胞对蛋白和基因产物具有独特和特异的翻译后加工和修饰机制。可选择合适的细胞系或宿主系统来确保正确修饰并加工所表达的异源蛋白。为此，可使用具有正确加工初级转录物(primary transcript)、糖基化、和磷酸化所述基因产物的细胞机制的真核生物宿主细胞。上面所列的哺乳动物细胞类型属于可用作合适宿主细胞的那些。
转染哺乳动物细胞并表达引入所述细胞的DNA序列的方法见例如Kaufman和Sharp，J.Mol.Biol.159，1982，pp.601-621；Southern和Berg，1982，J.Mol.Appl.Genet.1327-341；Loyter等，1982，Proc.Natl.Acad.Sci.USA 79422-426；Wigler等，1978，Cell 14725；Corsaro和Pearson，1981，in Somatic CellGenetics 7，p.603；Graham和van der Eb，1973，Virol.52456；和Neumann等，1982，EMBO J.1841-845所述。
其他真核生产系统也被本发明包括(envisage)，如在转基因植物或动物中制备所述融合蛋白。
本发明另一方面提供了如下制备融合蛋白的方法，其-制备了如上所限编码融合蛋白的基因表达构建体，-用所述构建体转化宿主细胞培养物，-培养所述宿主细胞培养物，从而表达所述多肽并将其分泌到培养基中，之后获得包含重组融合蛋白的培养基，并纯化所述融合蛋白。
用于培养所述细胞的培养基可以是任何常用的适合哺乳动物细胞生长的培养基，如含血清或无血清、包含合适添加物的培养基，或适合昆虫，酵母或真菌细胞生长的培养基。合适的培养基可得自商品供应商或可用公开配方制备(例如见美国典型培养物保藏中心目录)。培养基举例有添加了，例如，胰岛素，运铁蛋白，硒和胎牛血清的DMEM或RPMI-1640。
然后可将由细胞重组制备的融合蛋白从培养基中用常用方法回收，所述方法包括通过离心或过滤来使宿主细胞与培养基分离，用盐、例如硫酸铵来沉淀上清或过滤物中的蛋白性质的组分，用多种层析方法、例如HPLC、离子交换层析、亲和层析等来纯化。
优选实施方案中，如上所述用糖亲和层析来纯化所述融合蛋白。本发明优选实施方案中，用适合亲和层析的甘露糖，己糖的基质或N-乙酰葡萄糖胺衍生的基质来进行亲和层析。特别是使用那些已将基质经过甘露糖衍生的亲和层析。
本文中，纯化的重组融合蛋白被理解为用例如糖亲和层析从细胞培养物上清或纯化的转基因动物体液或组织中纯化得到的重组融合蛋白。
将所述培养基加载到层析柱中，然后洗柱子，优选用非-变性缓冲液洗柱子，所述缓冲液的组成，pH和离子强度导致除去蛋白但不洗脱出融合蛋白。此种缓冲液可以是TBS。用能够有效洗脱融合蛋白的选择性解吸附剂，如含有解吸附剂，如EDTA(例如5mM)或甘露糖(例如50mM)的TBS来洗脱融合蛋白，并收集融合蛋白。
药物组合物和治疗本发明所获得的融合蛋白可用于制备预防和/或治疗多种疾病或状况的药物组合物。本文术语“药物组合物”和“药物”同义。
除了所述融合蛋白，所述药物组合物可包含可药用的载体物质和/或赋形剂(vehicle)。
特别可加入稳定剂来稳定所述融合蛋白。所述稳定剂可以是糖醇(sugaralcohol)，糖(saccharide)，蛋白和/或氨基酸。稳定剂举例可以是清蛋白或麦芽糖。
可例如根据给药形式，将其它常用添加剂加入所述药物组合物。
一个实施方案中，所述药物组合物呈适合注射的形式。可使用常用载体物质，如等渗盐水。
另一个实施方案中，所述药物组合物呈适合肺给药的形式，如呈吸入粉末或局部给药的膏剂(creme)或液体的形式。
本文的治疗可包含治愈和/或预防，例如人的免疫系统和生殖系统和其他动物中起与此相似的作用的功能单元。要被治疗的状况不一定指现在已知需要治疗的状况，但通常包含任何与现有和/或预期需要有联系或与改善正常状况有联系的状况。特别是，所述治疗是对凝集素缺乏，如MBL缺乏的状况的治疗。
在本发明另一方面提供了药物生产，该药物由所述融合蛋白的药物组合物组成，用于治疗疾病包括治愈和/或预防疾病和紊乱(disorder)状况在内的情况，例如人的免疫系统和生殖系统和其他动物中起与此相似的作用的功能单元。
需要用本发明化合物治疗的疾病、紊乱和/或状况包含，例如治疗MBL缺乏的状况，治疗癌症和与免疫抑制性化疗相关的感染，包括特别是那些可见于与癌症治疗或与器官植入和/或移植相关的感染。本发明也包含治疗与习惯性流产(recurrent miscarriage)相关的状况。
因此，所述药物组合物特别可用于治疗和/或预防选自如下的临床状况感染，MBL缺乏，癌症，与化疗相关的紊乱，如感染，与人免疫缺陷病毒(HIV)相关的疾病，与先天或获得性免疫缺陷有关的疾病，更具体为慢性炎性脱髓鞘性多发性神经病(inflammatory demyelinating polyneuropathy)(CIDP，多病灶性运动神经病(Multifocal motoric neuropathy)，多发性硬化，重症肌无力(Myasthenia Gravis)，伊-兰综合征(Eaton-Lambert’s syndrome)，视神经炎(Opticus Neuritis)，癫痫；原发性抗磷脂综合征(Primary antiphosholipidsyndrome)；类风湿性关节炎，系统性红斑狼疮，系统性硬皮病(Systemicscleroderma)，脉管炎，韦格纳肉芽肿病(Wegner’s granulomatosis)，干燥综合征(Sigren’s syndrome)，青少年类风湿性关节炎(Juvenile rheumatiod arthritis)；自身免疫性中性粒细胞减少症(Autoimmune neutropenia)，自身免疫溶血性贫血(Autoimmune haemolyric anaemia)，中性粒细胞减少症；克隆病(Crohn’sdisease)，溃疡性结肠炎(Colitis ulcerous)，腹腔疾病(Coeliac disease)；哮喘，败血性休克综合征(Septic shock syndrome)，慢性疲劳综合征，牛皮癣，中毒性休克综合征(Toxic shock syndrome)，糖尿病，鼻窦炎(Sinuitis)，扩张型心肌病(Dilated cardiomyopathy)，心内膜炎，动脉粥样硬化，原发性低/无丙种球蛋白血症(hypo/agammaglobulinaemia)包括普通可变型免疫缺陷病(common variable immunodeficiency)，维-奥综合征(Wiskot-Aldrich syndrome)和重症联合免疫缺陷病(serve combined immunodefiency)(SCID)，在有慢性淋巴细胞白血病(CLL)和多发性骨髓瘤的病人中的继发性低/无丙种球蛋白血症，急性和慢性特发性血小板减少性紫癜(ITP)，同种异体骨髓移植(Allogenicbone marrow transferr)(BTM)，Kawasaki’s disease，和吉-巴综合征(Guillan-Barre’s disease)。
给药途径可以是任何合适的途径，如静脉内，肌肉内，皮下或皮内途径。本发明也包括肺或局部(topical)给药。
特别是可以给药所述融合蛋白来预防和/或治疗病人感染，所述病人患有与先天或获得性MBL缺陷相关的临床症状或具有患所述症状的危险。多种多样的症状会导致MBL-缺陷的个体对感染的易感性提高，如化疗或其他治疗性细胞毒性治疗，癌症，AIDS，遗传性倾向(disposition)，慢性感染，和中性粒细胞减少症。
因此可在开始给予化疗等之前和在化疗的至少一部分时间内时，给药所述药物组合物一段时间。
可将所述融合蛋白作为常用的“促进剂(booster)”在化疗前给药，或可将它给药那些只具有患MBL缺陷的危险的人。可通过在治疗前测量MBL水平来确定有风险的那组病人，并且只让那些MBL水平低于预确定水平者接受治疗。
将所述融合蛋白在化疗期以合适剂量方案(regime)给药，特别是通常以合适间隔给药，例如，每周一次或两次。
一般每个剂量给药1-100mg，如2-10mg，常常每个剂量5-10mg。常给药约0.1mg/kg体重。
本发明另一方面是本发明重组组合物在还包括其他药物的组合试剂盒(kit-of-parts)中的用途。特别是其他药物可以是抗微生物药物，如抗生素。
在流产的情况中，已经报道在另外有原因未知的习惯性流产的病人中，MBL血浆水平低的频率是增高的，这是为什么在这些病例中希望通过给药重组MBL来重建MBL水平，从而降低了易流产性。
至于本发明化合物的性质，看来本发明在最广泛的范围内涉及能够用作调理素，即能够增强吞噬细胞摄入的化合物，所述增强要么通过所述化合物和吞噬细胞的直接相互作用要么通过介导在靶表面上的补体沉积。
实施例实施例1FCNMBL-r1，-r2，-r3，-r4，-rS，-r6和-r7的质粒克隆1.1综述构建一系列质粒来在哺乳动物细胞中表达重组人甘露糖结合型凝集素2基因(rhMBL)和人ficolin 2(FCN2)的蛋白融合物。所述载体来自高拷贝数的基于ColE1的质粒并被设计来在哺乳动物系统中表达蛋白。所述融合蛋白表达由人巨细胞病毒(CMV)立即早期启动子驱动来提高组成型表达。在细菌中，通过受原核β-内酰胺酶启动子控制的氨苄青霉素抗性基因使选择成为可能。新霉素抗性基因通过SV40早期启动子驱动，它在哺乳动物细胞中提供了用G418进行的稳定选择。
1.2构建体和实验工作为表达Ficolin2和MBL的融合蛋白，我们设计并构建了一系列质粒。所述新的重组质粒是基于以前克隆的pcDNA2001-cintMBLcDNA。此质粒包含了合成的内含子和人MBL的cDNA。
设计了如下融合物(下划线字体表示FCN2部分-斜体表示融合蛋白的MBL部分)FCN2MBLr1(SEQ ID NO118)FCN2(信号序列(signalseq)+胶原蛋白+“铰链”到ficolin区氨基酸(aa)131)MBL(始于氨基酸129糖结合区(carbohyd.bind dom.))
MELDRAVGVLGAATLLLSFLGMAWALQAADTCPEVKMVGLEGSDKLTILRGCPGLPGAPGDKGEAGTNGKRGERGPPGPPGKAGPPGPNGAPGEPQPCLTGPRTCKDLLDRGHFLSGWHTIYLPDCRPLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPIFCN2MBLr2(SEQ ID NO119)FCN2(信号序列+胶原蛋白+“铰链”+包含预定的(pred.)卷曲螺旋到氨基酸207的ficolin区部分)MBL(始于氨基酸129糖结合区)MELDRAVGVLGAATLLLSFLGMAWALQAADTCPEVKMVGLEGSDKLTILRGCPGLPGAPGDKGEAGTNGKRGERGPPGPPGKAGPPGPNGAPGEPQPCLTGPRTCKDLLDRGHFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRVDGSVDFYRDWATYKQGFGSRLGEFWLGNDNIHALTAQGTSELRVDLVDFEDNYQFAKLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPIFCN2MBLr3(SEQ ID NO120)
FCN2(信号序列+胶原蛋白到氨基酸92)MBL(始于氨基酸101卷曲螺旋+糖结合区)MELDRAVGVLGAATLLLSFLGMAWALQAADTCPEVKMVGLEGSDKLTILRGCPGLPGAPGDKGEAGTNGKRGERGPPGPPGKAGPPGPNGAPPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPIFCN2MBLr4(SEQ ID NO121)FCN2(信号序列+部分胶原蛋白到氨基酸93处的cons.K)MBL(始于氨基酸77处的cons.K其余的胶原蛋白+卷曲螺旋+糖结合区)MELDRAVGVLGAATLLLSFLGMAWALQAADTCPEVKMVGLEGSDKLTILRGCPGLPGAPGDKGEAGTNGKRGERGPPGPPGKLGPPGNPGPSGSPGPKGQKGDPGKSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPIFCN2MBLr5(SEQ ID NO122)
FCN2(信号序列+部分胶原蛋白到氨基酸69处的cons.G)MBL(始于氨基酸64处的cons.G其余的胶原蛋白(包含“kick”)+卷曲螺旋+糖结合区)MELDRAVGVLGAATLLLSFLGMAWALQAADTCPEVKMVGLEGSDKLTILRGCPGLPGAPGDKGEAGTNGQGLRGLQGPPGKLGPPGNPGPSGSPGPKGQKGDPGKSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGIDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPIFCN2MBLr6(SEQ ID NO123)MBL(取代的MBL胶原蛋白(氨基酸41到氨基酸99)+卷曲螺旋+糖结合区)FCN2(插入的胶原蛋白氨基酸54到氨基酸92)MSLFPSLPLLLLSMVAASYSETVTCEDAQKTCPAVIACSSPGCPGLPGAPGDKGEAGTNGKRGERGPPGPPGKAGPPGPNGAPSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGFIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPIFCN2MBLr7(SEQ ID NO124)
MBL(信号序列到氨基酸25)FCN2(胶原蛋白到氨基酸93)MBL(始于氨基酸100卷曲螺旋+糖结合区)MSLFPSLPLLLLSMVAASYSALQAADTCPEVKMVGLEGSDKLTILRGCPGLPGAPGDKGEAGTNGKRGERGPPGPPGKAGPPGPNGAPSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPI用于所有构建的亲代质粒●pcDNA2003-cintMBLcDNA●Invitrogen Genestorm克隆RG000632(目录号(Cat.No.)H-K 1000Invitrogen)。
用BD的BD In-FusionTMPCR克隆试剂盒(目录号631774)通过重组进行构建。所述BD In-Fusion试剂盒可只基于载体和PCR产物末端之间2×15bp的同源性来克隆PCR产物。在用此试剂盒克隆时连接酶或磷酸酶是不必要的。所述In-Fusion酶捕捉DNA片段末端并将插入片段融合到所述载体。
用于PCR反应的引物见表1所示PCR反应和为重组进行载体线性化质粒“Genestorm RG000632”(批号N135-15C)在用Bstz17I消化后(N135-20B)进行PCR反应。用如下所述的引物对。PCR反应的试剂盒是PfuUltraTMHotstart PCR Master Mix Stratagene#600630。在BioRAD i-循环仪(cycler)上用表2所示的温度图谱操作PCR反应管。
为了进行重组反应，将载体pcDNA2001-cintMBLcDNA用如下所列的限制性酶消化来使其线性化。
FCN2MBLr1PCR所用引物Pr1-xho-MBLFCN+Pr4-Xmn-FCNMBL-rev(产物463bp)消化pcDNA2001-cintMBLcDNAXhoI+XmnI(部分)FCN2MBLr2PCR所用引物Pr1-xho-MBLFCN+Pr5-Xmn-FCNMBL-rev消化pcDNA2001-cintMBLcDNAXhoI+XmnI(部分)FCN2MBLr3PCR所用引物Pr1-xho-MBLFCN+Pr6-b-Bsp-FCNMBL-rev消化pcDNA2001-cintMBLcDNAXhoI+BspEIFCN2MBLr4PCR所用引物Pr1-xho-MBLFCN+Pr2-apa-FCNMBL-rev消化pcDNA2001-cintMBLcDNAXhoI+ApaIFCN2MBLr5PCR所用引物Pr1-xho-MBLFCN+Pr3-apa-FCNMBL-rev消化pcDNA2001-cintMBLcDNAXhoI+ApaIFCN2MBLr6PCR所用引物Pr8-BstAP-MBLFCN+Pr6-b-Bsp-FCNMBL-rev消化pcDNA2001-cintMBLcDNA部分BstAPI+BspEIFCN2MBLr7PCR所用引物Pr7-Alw-MBLFCN+Pr6-b-Bsp-FCNMBL-rev消化pcDNA2001-cintMBLcDNA部分AlwNI+BspEIIn-Fusion PCR重组反应用约50-100ng的Quiagen Minelute纯化的PCR产物和50-100ng的Quiagen Minelute纯化的线性化pcDNA2001-cintMBLcDNA进行In-FusionPCR重组反应。
将1/10的重组反应物转化到MAX有效性的(efficiency)DH5α感受态细胞(Invitrogen目录号18258-012)中。将每次转化的1/10和9/10涂布在不同的含有200μg/ml氨苄青霉素的LB平板上。平板在37℃温育过夜。
筛选阳性克隆从每个试验平板上选出至少6个集落来进行微量制备(miniprep)质粒DNA的分离。为确定插入片段的存在，用酶PstI限制性消化分析DNA。选择来自每个反应的三个阳性克隆来作进一步研究。
限制性分析为确认首次筛选后的所选重组质粒个体，我们对所分离的质粒DNA进行深入的(intensive)限制性酶消化分析。
用表3所示的酶消化所述重组体的质粒DNA。期望的片段也列在表中。所测试的所有重组克隆表现出预期的类型。EcoRI消化并非象预期的那样。在重组体和亲代质粒的消化中都见到一个额外的片段。此偏差可被解释为亲代质粒上多出了一个EcoR1位点。
结果所得到的重组质粒见图4-8的构建体r1，-r2，-r3，-r4，和-r5。
实施例2瞬时表达人MBL和人FCN2的重组融合蛋白的试验2.1概述我们报道了重组人融合蛋白FCNMBLr1，FCNMBLr4，FCNMBLr5和MBL在HEK293和Per.C6细胞中的表达。我们发现瞬时转染试验的细胞系至少能够产生以活性寡聚体形式组装的融合蛋白FCNMBLr4和FCNMBLr5，其结构基本上类似MBL寡聚体形式3和4。融合蛋白FCNMBLr4和FCNMBLr5在结合糖表面和激活补体级联反应方面的表现与MBL一样。
2.2介绍这些研究的目的是为了阐明制造由包含人ficolin2的胶原蛋白部分和人甘露糖结合型凝集素(MBL)组成的杂合蛋白的可能性。我们还希望明确是否此类分子仍具有结合到复合糖结构的能力并仍能够激活补体。
用两株人源的HEK293和Per.C6真核细胞系作为宿主细胞株来被各自的表达质粒瞬时转染。由CMV-IE启动子增强子驱动转录。
2.3试验材料和方法用于转染试验的质粒pME607-FCNMBL-r1，-r2，-r3，-r4，-r5，-r6和-r7(实施例1所述)所用细胞来源PerC6细胞从Crucell获得。
HEK 293 Freestyle细胞从InVitrogen获得。
培养基PerC6细胞在37℃，10％(vol/vol)CO2中维持无血清培养基中的单层。
HEK 293 Freestyle在37℃，8％(vol/vol)CO2中维持InVitrogen Freestyle培养基中的悬液形式。
转染并收获培养基用转染试剂Lipofectamine以DNA转染包含血清的培养基中的Per.C6细胞。转染一天后将所述培养基置换为无血清培养基。
用转染试剂293fect以DNA转染无血清Freestyle培养基中的HEK293细胞。转染后温育约4天后收集所述培养基。
MBL定量重组MBL试验(TRIFMA)使用甘露聚糖包被的平板或mAb-131-01包被的平板。为了定量MBL，进行时间分辨的免疫荧光测定法(time-resolvedimmunofluorometry)。
SDS-PAGE和Western印迹分析进行SDS-PAGE之后，将蛋白电转移到聚偏二氟乙烯(polyvinylidenediflouride)膜，并用单克隆抗-MBL抗体检测MBL。
C4试验该试验被设计用来测量MBL和rMBL启动补体系统MBL凝集素-途径的能力。与MBL相关的MBL相关性丝氨酸蛋白酶(MASP 2)裂解补体因子C4释放C4a和C4b。C4b在甘露聚糖包被的ELISA平板上沉积(disposition)，它可用生物素标记的抗C4b抗体和铕(Europium)标记的链霉抗生物素蛋白(Streptavidin)检测。
2.4结果在上述实验中，我们能够在无血清条件下在HEK293和Per.C6细胞中瞬时表达FCN2MBLr4，FCN2MBLr5和MBL。
寡聚体形式的融合蛋白用非-还原变性SDS PAGE然后是western印迹来检测寡聚体形式的融合蛋白。所述检测抗体识别MBL的CDR部分(可能还有卷曲螺旋区的一部分)。结果见图10所示。从该图明显可见，融合蛋白FCN2MBLr4和FCN2MBLr5最主要的形式是约250kDa，其对应于由三条蛋白单链(24kDa)组成的亚基的3体或4体形式。寡聚形式的出现不依赖于所用的宿主细胞。MBL产生了很多的寡聚体形式。
结合性质通过与甘露聚糖表面的结合并用识别MBL的CDR部分(可能还有卷曲螺旋区的一部分)的抗体进行检测，来测试融合蛋白的MBL糖结合区的功能。结果见表4所示。可以得出结论FCN2MBLr4和FCN2MBLr5完全与MBL一样在宿主细胞中表达，并且所述融合蛋白结合甘露聚糖表面。
MASP-2结合和C4裂解进一步检测所述融合蛋白结合MASP-2和激活MASP-2的丝氨酸蛋白酶的能力。这可以通过测量所述融合蛋白与甘露聚糖表面结合导致的MASP-2底物补体因子C4的裂解来进行。结果见表5所示。从这些结果可以得出结论融合蛋白FCN2MBLr4和FCN2MBLr5保留了结合并激活MASP-2的能力。
讨论上述结果清除阐明可以构建FCN 2和MBL的融合蛋白，其具有如下性质1.所述融合蛋白的寡聚体结构比MBL蛋白的结构更简单。
2.所述融合蛋白保持MBL通过与致密糖结构结合而激活补体级联反应的基本性质。
表1用于PCR反应的引物黑体序列显示在载体中重组所需的15bp同源性
表2

表3

表4 TRIFMA所测的MBL与甘露聚糖的结合

表5 融合蛋白的C4活性

权利要求
1.融合蛋白，其包含a)源自凝集素-补体途径激活蛋白或其功能同系物的第一多肽序列；和b)源自胶原凝集素或其功能同系物的第二多肽序列；其中所述补体激活蛋白不是胶原凝集素。
2.根据权利要求1的融合蛋白，其中所述第一多肽序列能够激活所述凝集素-补体途径。
3.根据权利要求1的融合蛋白，其中所述第一多肽序列能连接至少一个MASP蛋白。
4.根据权利要求1的融合蛋白，其中所述第一多肽序列能连接选自由MASP-1，MASP-2和MASP-3组成的组的MASP蛋白或其功能同系物或变体。
5.根据权利要求1的融合蛋白，其中所述补体激活蛋白是ficolin。
6.根据权利要求5的融合蛋白，其中所述ficolin选自由L-ficolin，H-ficolin和M-ficolin组成的组。
7.根据权利要求5的融合蛋白，其中所述ficolin是L-ficolin。
8.根据权利要求1-7任一的融合蛋白，其中所述第一多肽序列包含补体激活蛋白的至少10个，如至少12个，例如至少15个，如至少20个，例如至少25个，如至少30个，例如至少35个，如至少40个，例如至少50个连续的氨基酸，或与其至少70％，如80％，例如90％，如95％相同的序列。
9.根据权利要求1的融合蛋白，其中所述第一多肽序列包含ficolin的胶原蛋白样区或其功能同系物或变体。
10.根据权利要求1的融合蛋白，其中所述第一多肽序列包含L-ficolin的胶原蛋白样区。
11.根据权利要求1的融合蛋白，其中所述第一多肽序列包含ficolin的半胱氨酸富集区或其功能同系物。
12.根据权利要求1的融合蛋白，其中所述第一多肽序列包含L-ficolin的半胱氨酸富集区。
13.根据权利要求1的融合蛋白，其中所述第一多肽序列包含ficolin的半胱氨酸富集区和胶原蛋白样区或其功能同系物或变体。
14.根据权利要求1的融合蛋白，其中所述第一多肽序列包含L-ficolin的半胱氨酸富集区和胶原蛋白样区。
15.根据权利要求1的融合蛋白，其中所述第一多肽序列包含SEQ ID.NO 1的氨基酸1-77。
16.根据权利要求1的融合蛋白，其中所述第二多肽序列能连接一个或更多个糖。
17.根据权利要求1的融合蛋白，其中所述胶原凝集素选自如下MBL(结合甘露糖的凝集素)，SP-A(肺表面活性蛋白A)，SP-D(肺表面活性蛋白D)，BK(或BC，牛共凝集素)和CL-43(胶原凝集素-43)。
18.根据权利要求17的融合蛋白，其中所述胶原凝集素是MBL。
19.根据权利要求1-18任一的融合蛋白，其中所述第二多肽序列包含胶原凝集素的至少10个，如至少12个，例如至少15个，如至少20个，例如至少25个，如至少30个，例如至少35个，如至少40个，例如至少50个连续的氨基酸，或与其至少70％，如80％，例如90％，如95％相同的序列。
20.根据权利要求1的融合蛋白，其中所述第二多肽序列包含胶原凝集素的CRD区或其功能同系物或变体。
21.根据权利要求1的融合蛋白，其中所述第二多肽序列包含MBL的CRD区。
22.根据权利要求1的融合蛋白，其中所述第二多肽序列包含MBL的颈区。
23.根据权利要求1的融合蛋白，其中所述第二多肽序列包含MBL的胶原蛋白样区。
24.根据权利要求1的融合蛋白，其中所述第二多肽序列包含MBL的颈区和CRD区。
25.根据权利要求1的融合蛋白，其中所述第二多肽序列包含MBL的胶原蛋白样区，颈区和CRD区。
26.根据权利要求1的融合蛋白，其中所述第二多肽序列包含SEQ ID.NO 2的氨基酸80-228。
27.根据权利要求1的融合蛋白，其中所述融合蛋白包含L-ficolin的半胱氨酸富集区和胶原蛋白样区以及MBL的CRD区。
28.根据权利要求1的融合蛋白，其中所述融合蛋白包含L-ficolin的半胱氨酸富集区以及MBL的胶原蛋白样区，颈区和CRD区。
29.根据权利要求1的融合蛋白，其中所述融合蛋白包含SEQ ID.NO.3所限的氨基酸序列，或其功能同系物。
30.根据权利要求1的融合蛋白，其中所述融合蛋白由SEQ ID.NO.3所限的氨基酸序列组成。
31.分离的核酸，其包含编码根据权利要求1-30任一的融合蛋白的核苷酸序列。
32.包含权利要求31的核酸序列的载体。
33.包含权利要求32的载体的细胞。
34.根据权利要求33的细胞，其中所述细胞是哺乳动物细胞。
35.根据权利要求33的细胞，其中所述细胞是非哺乳动物细胞。
36.根据权利要求1-30任一的融合蛋白，其用作药物。
37.在有需要的个体治疗临床状况的方法，其包括给药所述个体根据权利要求1-30任一的融合蛋白。
38.根据权利要求37的方法，其中所述临床状况是感染。
39.根据权利要求37的方法，其中所述个体是人。
40根据权利要求37的方法，其中所述个体是感染风险增加的人。
41.根据权利要求37的方法，其中所述个体是MBL血清水平低于正常的人。
42.根据权利要求37的方法，其中所述个体是MBL血清水平正常的人。
43.根据权利要求1-30任一的融合蛋白在制备用于治疗有需要的个体的临床状况的药物的用途。
44.根据权利要求43的用途，其中所述临床状况是感染。
45.根据权利要求43的用途，其中所述个体是人。
46.根据权利要求43的用途，其中所述个体是感染风险增加的人。
47.根据权利要求43的用途，其中所述个体是MBL血清水平低于正常的人。
48.根据权利要求43的用途，其中所述个体是MBL血清水平正常的人。
49.用于治疗或预防有需要的个体的临床状况的药物，其包含根据权利要求1-30任一的融合蛋白。
50.根据权利要求49的药物，其中所述临床状况是感染。
51.根据权利要求49的药物，其中所述个体是人。
全文摘要
本发明涉及能够激活补体系统的融合蛋白，所述融合蛋白包含源自凝集素－补体途径激活蛋白或其功能同系物的第一多肽序列；和源自胶原凝集素或其功能同系物的第二多肽序列；其中所述补体激活蛋白不是胶原凝集素。优选的融合蛋白包含图1的L－ficolin序列的氨基酸和图2所示MBL序列的氨基酸。所述融合蛋白适用于治疗中，包括产生，重建，增强和/或刺激所述补体系统的调理和/或杀细菌活性，即增强免疫防御识别并杀死微生物病原体的能力。因此，本发明涉及包含所述融合蛋白的药物，生产所述融合蛋白的方法，和治疗疾病，特别是感染的方法。
文档编号A61P31/00GK1694961SQ03825026
公开日2005年11月9日 申请日期2003年9月10日 优先权日2002年9月10日
发明者利夫·康格斯莱夫, 迪特马·韦尔吉尼, 芬恩·马西森 申请人:内蒂穆恩公司