人类肝脏中表达的表达序列标签a组的制作方法

专利名称：人类肝脏中表达的表达序列标签a组的制作方法
技术领域：
本发明涉及生物技术领域，尤其涉及一类在人类肝脏中表达的表达序列标签。
背景技术：
肝脏是人体内最大的消化腺。也是体内新陈代谢的中心站。据估计，在肝脏中发生的化学反应有500种以上，实验证明，动物在完全摘除肝脏后即使给予相应的治疗，最多也只能生存50多个小时。这说明肝脏是维持生命活动的一个必不可少的重要器官。肝脏的血流量极为丰富，约占心输出量的1/4。每分钟进入肝脏的血流量为1000-1200ml。肝脏的主要功能是进行糖的分解、贮存糖原；参与蛋白质、脂肪、维生素、激素的代谢；解毒；分泌胆汁；吞噬、防御机能；制造凝血因子；调节血容量及水电解质平衡；产生热量等。在胚胎时期肝脏还有造血功能。
肝脏疫病分为肝炎、肝硬化、脂肪肝、肝癌等。现代医学实验证明，肝病病毒侵入人体后，并不直接引起肝细胞的损害，只是在肝细胞内吸收营养赖以生存，并在肝细胞内复制、繁殖。其复制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)释放在肝细胞膜上，引起人体免疫系统对这些抗原物质产生免疫反应，这种反应造成肝细胞的损伤、坏死。免疫反应的强弱决定于肝脏受损程度及临床症状轻重。这场由病毒引发的、免疫系统对肝细胞的战争，使大约25％的患者的肝脏成为战火连绵的战场，肝脏的损伤由此加重。肝病的危害绝不仅仅限于肝脏本身，它还可以引起其它多种疾病。常见的有(1)糖尿病；(2)胰腺炎；(3)胆道感染；(4)功能性肾衰竭；(5)胆汗性肾病；(6)肾小球肾炎；(7)肾小管酸中毒；(8)溶血性贫血；(9)再生障碍性贫血；(10)心肌炎和心包炎；(11)结节性动脉炎；(12)消化性溃疡；(13)自发性腹膜炎；(14)性激素代谢紊乱；(15)甲状腺功能改变；(16)肝性骨病，等等。肝病不仅对患者的身体甚至生命造成危害，而且对患者心理上的打击也是十分沉重的。无论是肝病患者还是病毒携带者，在生活、社交、求职、升学等方面都会受到严重影响。
生物基因组中可转录表达的序列(即基因)仅占总序列的3-5％，对这部分序列进行测定，将直接导致新基因的发现，并获取基因组中与产业化关系最为密切的信息。20世纪80年代，高通量的自动测序的出现，使从质粒互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库随机选取许多cDNA克隆和决定来自非载体两端的几百个碱基的DNA序列成为可能。这些短的DNA序列叫做“表达序列标签”(Expressed Sequence Tags，简称ESTs)。表达序列标签的概念最早是由Adams等在1992年提出来的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics，2，173-179)针对获得大量信使核糖核酸(mRNA)序列的迫切需要，提出大规模互补脱氧核糖核酸(cDNA)测序的研究战略。随后Venter创立了大规模表达序列标签技术。其基本特征就是从以质粒为载体，构建完成的目的组织互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库中，随机选择许多cDNA克隆，利用质粒上携带的通用引物对cDNA两端进行一轮脱氧核糖核酸序列测定，所获得的来自3’端或5’端的几百个碱基的非载体短脱氧核糖核酸(DNA)序列。简而言之，表达序列标签是来自表达基因片段3’端或5’端的短脱氧核糖核酸序列，代表一个表达基因的部分转录片段。
表达序列标签可用于新基因克隆、人类基因组图谱绘制、基因组序列编码区的确定等。如果一个表达序列标签在基因组中只出现一次，那么它可以作为序列标签位点(STS)。由表达序列标签构建的物理图谱叫表达图或转录图(expression ortranscript map)。利用表达序列标签进行基因图制作，可以加快序列标签位点的制作和新基因的染色体定位。表达序列标签可以作为基因特异性探针，对组织特异性基因表达的研究具有重要的作用。表达序列标签还可以进行新基因的遗传进化关系分析。表达序列标签可以对所有动植物的基因作为一种数据库，通过不同的序列比较可以获得保守序列片段，从而获得基因的遗传进化图谱。正因为表达序列标签具有如此的优越性，因此表达序列标签测序已经成为许多基因组研究机构的工作重点。
表达序列标签(EST)是一种快速有效揭示基因组容量的方法。ESTs在新基因的发现、基因作图和基因组序列编码区域的确定等方面起到重要作用。EST不仅为基因组遗传图谱的构建提供了大量的分子标记，而且来自不同组织和器官的EST也为基因的功能研究提供了有价值的信息。此外，EST计划还为基因的鉴定提供了候选基因(candidantes)。
在本发明之前，还没有出现涉及本发明的一类在人类肝脏中表达的表达序列标签的公开报道。

发明内容
本发明要解决的技术问题是提供一类在人类肝脏中表达的表达序列标签。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明的一个方面，提供了一类在人类肝脏中表达的表达序列标签的序列，其包括(a)SEQ ID No.1～SEQ ID No.55所示的序列；(b)SEQ ID No.1～SEQ ID No.55所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.55所示的序列中每条序列有至少70％同源性的序列；(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.55所示的序列。
本发明还提供了一种探针分子，所述的探针分子含有上述序列中约8-100个连续的核苷酸。
由本发明的一类在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中特异表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物中发挥重要作用。
具体实施例方式
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不是限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件如Sambrook等人，分子克隆实验室手册(New YorkCold Spring HarborLaboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1人肝脏组织的mRNA的分离组织分离(Tissue isolation)肝脏来源于5个成年男性，在肝脏切除手术后，将肝脏组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation)取出肝脏组织，用研钵研碎，加入盛有裂解液的50ml管，充分振荡后，再移入玻璃匀浆器内，匀浆后移至50ml新管，抽提总RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligod(T)的纤维素柱分离总RNA中的mRNA，定量。
实施例2cDNA文库的构建(Constuction of cDNA library)以mRNA为模板，合成双链cDNA。补平末端后，加含EcoRI切点的接头。磷酸化EcoRI末端后，用XhoI限制性内切酶消化1.5小时，再进行片断分离。过柱筛选长度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，无菌水溶解，连接至Uni-ZAP XR载体(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)进行包装，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)细菌。涂板并测定滴度。
实施例3测序及数据库建立(Seqencing and Database Constructing)挑选文库中有外源片段插入的克隆，扩增后抽提质粒(Qiagen Germany)，用T3和T7作为3’和5’端的通用引物，采用终止物荧光标记(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377测序仪(Perkin-Elmer，USA)上进行EST大规模测序。测序结果用FACTURA软件去除载体序列，传输到SUN Ultra 450Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group，USA)中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL)，将无同源性或同源性低于95％的序列视为新基因建立数据库。
实施例4基因的全长克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基础上，进行cDNA全长克隆，分两阶段进行(1)“电子克隆”(Electronic Cloning)以新基因片段序列作为探针搜寻dbEST数据库，将重叠序列＞50bp，同源性在98％以上的表达序列标签(Expressed Sequence Tag，简称“EST”)序列认为同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER软件进行连接，部分EST可以延伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(OpenReading Frame，ORF)，用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸和氨基酸水平上是否与其他物种有同源性，以帮助判别所得到的基因全长完整性如何。通过电子克隆的方法，通常可获取人肝脏相关基因的全长序列。
(2)cDNA末端快速扩增(Rapid Amplification of cDNA Ends，RACE)如果通过“电子克隆”方法仍未得到完整的cDNA全长，则在已有序列5’或3’端设计引物，在人类肝脏Marathon-Ready cDNA文库(Clontech Lab，Inc，USA)中进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软件分析被延长的序列有无完整的ORF，如无，重复上述过程直至获得全长。
(3)RT-PCR对于5’和3’端的已知的序列，如果中间有一段间隙(gap)无法从已有的公共数据库或自身数据库获得，可考虑采用RT-PCR的方法。在序列5’端设计引物，3’端引物采用Oligo-dT，在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最后拼接便获得全长。
通过组合使用上述3种方法，可获得人肝脏相关蛋白的全长编码序列。
序列表<110>上海人类基因组研究中心<120>人类肝脏中表达的表达序列标签<130>NP-10048<160>55<210>1<211>550<212>DNA<213>Homo sapiens<400>11 catcgttttt gtttttattt ttcccctttc aacctggttt tcggctctgg aagctgttcc61 tgtgcacagt tctatttcct acagttctga gaaagcggtt ccatcgcaga tgaccaaagg121 ctggacccct ctggctataa cgtagacctt ccagtgggtg gtgtcgacat agccgatatc181 ccctggctct attggcatgg ccacagactg acaatgtctt cggcactgga agggagggat241 gagctgcaac atggcctcac cgctgccaat gataaatagg tcanggcatc ttatgcatct301 tactccccac aacaggttct gggtgaagaa aggagcactt gtttaaagag gcatcttttn361 ggaaatgnca ggtgagtgaa aacaggaaaa agctcacgta acatttgtna tctgttcana421 ggtctcctcc atggataagg naatgggaac caccgctcag gaaagcagcg ccaccacttg481 tcgaaaaaac ctttcaggag aangccttct gtcgnctcag gggtactggc acanaaaaat541 gctgaatctc<210>2<211>410<212>DNA<213>Homo sapiens<400>21 atggggtttc accatgttgg ccaggctggt cttgaactcc agacctcaag tgatctgccc61 gcctggcctc ccaaagtgct ggggttacag gcgtgacacn tgcgcccggt caggctttta121 atttttttaa tttttaattt ttgctcaggg tggtttggct attctgggcc ttttgtggtt181 ccatataaat tttagggtta ttgtttctat ttctgcgaag aatggcattg atattacagg241 gagcccacgt agccctttac attgtaattg gcacctccac caggccataa gaccccaggt301 cccagcccag aagcatgtcc cagaggaaag actcagagaa gctcctggaa tgtgtgtgca361 cggctccttg gtattgcttn gggccttcac ccctttggtg gccactttcc<210>3<211>553
<212>DNA<213>Homo sapiens<400>31 tgccagcatt tccctttatt tctggatgga aacggggccc taaaagcaga aatcaatatt61 tttgtttgaa agatgcagtc atgctaattt cacttttggc taaaaccgag acgataaaag121 aacagttggg tgtttatagg atgccctcaa agtgagctgg ctaagtgagc tgggctctaa181 cttcactcac aaatttatag tacagctaag aaggccagtc tgtccatgaa agggagccga241 gacaagacga gggcggcctc ttccaggcct gtgccaagtg tccttggggt cccgccatgg301 tccacacttc tgcagcatcc gcagaacatg tggccggggt cctgcccagc agcagggaca361 gcccaagtgg ggaggcaggc atggtgcaca ccctggggag gcccctgggt gcagaagcag421 ccccacagta gcagccccat ccagaggaag accactcccg aggggccaca gccttcttgc481 agcccctngg aattgccgcc caacccctcc attttcagcg ggatgtttca agggtgagac541 aaggaattna ggg<210>4<211>767<212>DNA<213>Homo sapiens<400>41 gagaatcaat acaaaatatt tatttttttt caaaccacag aattcttaac cccagagcca61 cacaataaag ttctcagaat tgtaagccat taacattttt ctaaacaatg cagtccagag121 atgaagataa tttccaacca gcagggatgc aatatatagt aggttcccct atgaatgaag181 ctcaaattag catttccttt aattctccca cagccactcc atcaacagaa gcagaaacag241 tacacatatt catgccactc ggctctgaaa agaggttcaa ggtgggtcaa ggtgggtctt301 ggccagtgga ggaaggaagg tgtccaggac tttagttaat caacagtggg gacagagagg361 aatgatttcc cttggaaaac aacagggttc ctttctcata ttcttgtggc cagaaactgg421 ggtgaacttc agtggggtaa tgaaagaaac aggagagcca tttctccagg aactcctatg481 acctccattt taacttctga caaagttaac ttcattatac aatcgtattg aaaacagtaa541 tcacaaccaa aaggtctata acctgtaata gtgtcaaggg atcncattcc ggactttaat601 ttaaggnccc ttaaaagcct agcctggtta agtaccgaat attcccagct aagnggcctt661 aaatccccaa catccaccat ggtnagctag tngtgttcnt tgtgnacctt cggtncgatg721 ggggnttaaa agccggtttt tnggtgaaaa agttnctagg ggacttt<210>5<211>468<212>DNA<213>Homo sapiens<400>51 tgcagtttga atttatattt tattattttg aaatacaaaa atttaaaaac atttcaaaat61 acaccagtac atgtttgttg taaaaatttc acctaggagg atataaaata gaaaagccga121 atattccttc taactctccc ctctacctca attcccttca gaggtaacac agcaaaaaaa
181 aactggggaa aagcaaacaa ctttccagac ctctactatt ctaaaacaca aagagcatta241 cagcttgcta tgcatttttt tttnnttnng gggggggttg ttttttagag acggagntcc301 cctctgtcac ccaggctgga atgcagttgt gtgatctcgg ctcactggaa cctccacctc361 cccagttcaa gcgattctcc tgcctcagcc tgccgagtag ctggggatta cagggcangc421 gccaccacgc ccagctaatt tttgtagttt tagtagagac agcgtttg<210>6<211>605<212>DNA<213>Homo sapiens<400>61 tatgaaaaaa gcagcaactc tttagtgatc atggaattaa tctgacagca attaaatgtg61 tttaagcatc tggcatatct cctcaattgc accaaaagaa tttggaagca cttggtttgg121 tctcaaaggc aaaaggaaag gacgaggaag gggccaggct cccgccagcc cccgcccccc181 tcacatttct gagtccgcat acatcccgtt gattaagtag tccacctggg tgtagtcctt241 cttcttgtag ctctcatagg cctgcaggna aacaaaacca agactgtgat gaaaagggtc301 accccgagta acagcaccac cagaaggaga gttttgtcta ccacggcctg gggtgagggg361 ctcagtggtg accaccatgt actgggcctt gggtgctggg ctggcacgag tccgtggctg421 gcatcttagt gcccctgagc tcctgggtgt tgggcccagt ggaatcagta cctggtgtgg481 cttcagtctc tacctgctcn ggtgcctggg atgtgcctgg cccagcggcc ctctgggtat541 atggcatggg gcttggntgt gtcgtggggg acatgncctc catctcaggg attgggctcg601 tccga<210>7<211>660<212>DNA<213>Homo sapiens<400>71 caaatntaan acccccntnn nanttncccc ccaannggta cttttgtttt acaaaaatta61 caaaaaatga ctcttaaaaa tagaaatagc ttctcagact cttgggaact acttttaagc121 aaccaaggag ttagaactca gatggttttc ttctttgtaa cagcaacaat gaccacggct181 ctgtgccatg gcaatcactg ctgagtgtct tctcctacaa ttacacaggc ccgagctgtg241 tctggtggaa aggtctccac tcaggtcaga gtctgaagga gcaagatctt ctcattaatg301 cagaacctgt gcagaaccac catcagattc tctccacagc gggagacctg gcgctccatg361 gccaggcgaa agtcctcctt cacgcctttg gtgatgcctc gagttacagt gtggtgcctg421 aggtcagcaa tagggaaaca gacaaagacc aggggttagc accagcatcg ctgatcgctg481 ctacttgggt aggaataaag cctgccccat atctaagtat aatttcagat attttaaaaa541 gaaccaagaa tagctgcacc aaccaaacat tccagccatt tctcttcaga agctttaact601 agacagacat cttctcactc tataaaggta cttcaaacat ttattgtcag taaatgcncc<210>8<211>611
<212>DNA<213>Homo sapiens<400>81 acatttaaga catatttttg tgtttcttaa tgaataaatt atgaaagaat gttaatttaa61 aattcataat atgtaaaaga atatttagat ttcatttcct ataatcagac acgctgttgt121 ttttactata taactgttag aagatataaa tctataacat atgcagcata tacataaaaa181 taatcacaat atttttaaaa gtttctgtta atgtcatgta tcttcttttt ttatccaaaa241 gaaaacttat tcaaaaaaat gtacccagaa gacacagcct aaggaataca agcaggaaac301 tgagaaacct gccaacaaca gctaaaggga cacaaacagg tacatgcaaa gttgccagta361 agcagcattt aaaatgctga ttttcagaaa gagagcagca ggtggagtta agggtttagg421 ctctggcgtc gactacttag ggacgttggg aaagtttcta ccctctctgt accaagtttt481 ctctttgtaa aatgggaacc aatccttctt gctttttttt tttttgagac ggatcttgct541 cggttgccca gccttgagtg cagtggcgca atctcactcg ctgcaacctc cgcctcccgg601 gttcaatgga t<210>9<211>596<212>DNA<213>Homo sapiens<400>91 ttcttgtggc aaatttaata gaagaatatg agactcaccc ttacttccca cacataaaac61 actgcaggca ctccaaatcc ttacagacat atgcacttcg gaatcaactc aggcatgcac121 agcatccctg tgctggagtt tattttaaaa aacaacgccc cagttatcac agtttctttt181 tttgtccacc attttccata acaaaagaag ctacacaaaa tttgggggga gatactctct241 ttggagactg acacatttgc agaggggtca tgaataatga ttccaaagct cctatttaac301 ttctgaatca ggcaaagaat aagtgacaat ataagaatga attttgttta cagcaatatc361 ataatacagc attgaatcat tacagtggca gtggttgtaa ttaagtcccc ataaaatatt421 cttaggcccg aaacaccaga ccaactaaaa tcaccaactg gctcacgaac caaatgatca481 gtgcaagcag ttgnggtctc catagtgaaa accaaaatgg gtagggcntt tctgggcagt541 gggtctctga catgaacggt ccctctctgc tcaagangac ttggcgnccc ccttgg<210>10<211>607<212>DNA<213>Homo sapiens<400>101 ggagaagtct agagtgtttg ctttattcat gttgtttatg cgttaaaata taagcatatt61 gcatacataa gcatatttac acccacatct aaaagataac atattatata tacttttcca121 tacttctgaa caagcgtctc atatccataa ctaaaaatta gatgagaaag acaaagaact181 cttgtcttcg aattgacttc attgcactat atagcatttc acactgcggt tccaggccac241 agggtccctt gggtaggggg agacaaaggg tgtggctgac agggagactc taggactacc
301 attgccactt ccaccagagc agccacactt ctgttccaca tagttggcat ccaaatttga361 tttgaaaaac agtttccact tacctaaaag aaaaatccta tgngaaaaat gaccacaagc421 caattatcca ctcnaacctg ctgatnctgg gccctgggga gaagctgttt tgagaccnca481 tatttcccta ttacctttac ccatgatggg attatcctac tgtctngggg gcatccccaa541 cttgagctcc ccagaaaccc ccnggtggtg aggggaaaaa aaaaaccctt ctcccattca601 cagtggn<210>11<211>665<212>DNA<213>Homo sapiens<400>111 gtatttttaa aatttttatt gtattttttt tcagaatatt ttccatccac agttggttga61 acagagtgcc atctgcacct agaatgctgt ctcagcccaa ggtaccttct tcacatcatt121 catgaagtgc tttaaaccac tccccctcaa gaagcccttt agaacactca gaaaaagatc181 ctcatctcat aattcttggt ccaaatacaa aactccagac tcctcaatta ctgcatcact241 aaaattccca ccatttcagt gcatataatt agctttttaa gttatatatt tattattagc301 ttggtgtttg tgtgtttcca tcagtggtaa caccataaac atgattcttc caattttact361 acttctgtaa cacagttgca tgtggaaaac ggacattcga taaatttatc ctgacttcac421 atggccaaat agtaccttac acagccctca gttaaagcca ccagtttata agtaactagt481 ggaatagcct agaggtgaca aaggaagagt tgggcagcag cattcaacaa acatccgtga541 atgaaacttg ctcctccatt ccgatatctg ggctgcactg atctaccact ctccggtttc601 tgcntcgttc tncctgcttg cattagaatc agctcgtgtt aaagtgggcc gaaagacaat661 tcccc<210>12<211>529<212>DNA<213>Homo sapiens<400>121 cttgagacta gtggccagtt caaaaattaa tagccgctca ttaacaattt ttgggacggg61 agacacccta aaagccatca caatgactgc taatagtggt tttgtcagag ctgcaaacaa121 ccagggtgtt aaatttagtg tgtttatcca tcagcccaga gcacttactg agagtaaaga181 ggctctagcc acccccttac caaaagacta cacaactgac agtgaacact aaagataccc241 tttaaatatt tttgtggaag taagttattt ataccaaagg ctgggtcttc ccatctaaac301 tggtgggaaa aaaggtctat agtaaaagta aaactgagaa ctcttaaacc agtagagttt361 ctttgtctct catctaaagt atataacctc ccaaagtaga aaaagcacaa gagagaagct421 tatctatcaa actgtctgca ttagtgcaat taagaaccga aatacgtcta tataaagctt481 tcagctgtat tgtaatactt tgattcgata gggctacaac ttccctact<210>13<211>480
<212>DNA<213>Homo sapiens<400>131 tttcaaattt ttaatgtacc aatttattgc atatgtcagt acagaaaaat agaaagtata61 ttttaaaaga aatttattgt aaaaaacaaa tgtaaatgtt tgcttgccat ggttagcaca121 caatgtacta tgacattcaa atttgagtta taaaatttta tcacttcaaa taaaatatgg181 caaataacaa aaatatgatt tacattatga aatgggatta acttatttta tactgaacat241 acttgaatat gttaaaaaca acttgaattt tccatatacg ctgtactgac agaaataatt301 ataaattatg ttaaacacac actcacctgt tacatatgta tatacggcac tttttgtaaa361 aaagtgtctc atacatggtc ctatttttaa actacatttt aagatacaaa atataaatgg421 aggtatattt gcgccatttg gcaaaaatgt atagactagt attaagatga ctgcatttat<210>14<211>551<212>DNA<213>Homo sapiens<400>141 tttgatatca aaatgcatct tgtttatttg gtttttgaaa ctgttataac acaacttata61 acattggaaa tacattattt tattaataca aagtagtttt catagtaaaa taccaaatta121 attaaaaggc agaatgaaac gtaccatata catgtagacg acaaatgaaa actgtcaaag181 tatccttctc tctaaaaagc cacaaattac attctttgaa gtaagtattg ggttttattt241 cctaaactat acacatcaat atcacctcct gaagcttttt acccagtttc acggttggta301 tttcggacat gcaatgttcc atgctgtcaa acttggaatg cctcgtttgg atacagggag361 acaaagactt ggagacccag aagcctgaaa gtggttcttg accgactttg tccactcaaa421 ttaaggcatc atgcagctgg tcatcactta cattgctggt tgcacgcagg cacacacaca481 ccagtgctca cagccagcac ttgttaagaa tggttgagct ggaccaaatg gtatacgtgt541 ctttcatcaa t<210>15<211>534<212>DNA<213>Homo sapiens<400>151 gccgacagtc cctccttgtc tgtctccggg attcaggcct ccctccctga catggagagt61 aacctgtctg gcctggtgcc tgctgccggg ctggtgcctg cgctgccacc tgctgtgacc121 ctggggctga cagctgccta caccaccctg tatgccctgc tcttcttctc cgtctatgcc181 cagctctggc tggtgcttct gtatgggcac aagcgtctca gctatcagac ggtgttcctg241 gccctctgtc tgctctgggc cgccttgcgt accaccctct tctccttcta cttccgagat301 actccccgcg ccaaccgcct ggggcccttg cccttctggc ttctctactg ctgccccgtc361 tgcctgcagt tcttcacctt gacgcttatg aacctctact ttgcccaggt ggtgttcaag421 gccaaggtga agcgtcggcc ggagatgagc cgaggcttgc tcgctgtcag aggggccttt
481 gtgggggcct cgctgctctt tctgctggtg aacgtgctgt gtgctgtgct ctcc<210>16<211>627<212>DNA<213>Homo sapiens<400>161 ttttttttta aaaattattt tatttatgta tgcattttat aagcaactga ttaatttaaa61 ctgaaaaatg atgaagtaaa ggcctacagc tctaagaatc atttctaggc tgggcgtggt121 ggctcacacc tgcaatccca gcacttgggg aggctgaggc aggtggatca aactgaggtc181 aggagttcga gaccagcata ttttcttcca aagccatcat atatatatat atatatatat241 atatatagat acgaggaaac aganagagat acaaaagtgc atagatgtat atatagagtg301 taaggtaatc agggatatat tgattcaggt gggacatgtt gcgaaagagc ggttgttgtg361 aggtgagggt ggtggcgcgc gatggattcc tggagtgttt acttatccgt gcccgagacg421 cccatgccgt gtttgtgtac ctggtccccg gggtcccaac cactttgccg tgggacgcca481 ggagtaacgc ctgatgtcgg aagaatagat gtgtgttgtt gtggtagtgt tgtgttgggt541 gacgtgagag aggtgctgag gcgcatacgt agtaatatag gctggaagag agtaccttgt601 gagaagtgtg agcggagacg cctagta<210>17<211>537<212>DNA<213>Homo sapiens<400>171 catgtttttc ttttaataag ggtttttact caaaagtgta gctttgaaaa tctctagctt61 gttgtgaaaa ccaggaaagc caaggagctg cctcacactg cacaccacca cgtgaagctg121 tgggccgctg tgtgtttgca aactctccat taccaaggct ggaatcaaat gcaccacttt181 aaaatcagag ccaacattta aaattaggag gatgatgcat gtcctacttt gattcaagga241 gaaaggacaa gttcttagta cagtaaaaca aaacacaaaa gtaaacaaat ctttagaaat301 cactatatat atgtgtgttt atatatatat ttttatataa ttaggattat catcatttca361 aactattaaa aataaggttg ccaccttacc ttttcttttg gtaatggggt gttatttttt421 ttaagagaaa aaaccaaaca ttttgcccag actctagagt tcccaacttc aattctgttt481 tctgctgtgc acttccccac cttcacgctg tatcccaagt ccgccaccat tctggct<210>18<211>485<212>DNA<213>Homo sapiens<400>181 tatttcaact tttattaaac caaatccagt aacttttaca atgccttact agagggttgc61 ctcataactg aaccttcatt tctggcaggg aagaaaccat ggcttcagga tcatgactga
121 agtttggcca tgttccatca ttaatgttcc aacatcacca gggacacaaa gctgcaaaaa181 tgagaaggga aataaggtta gagaaaggat ctgggcaatc ttaaggactg aggaagacat241 gttccccaac ccttgaactc acaaaccctg aagctcaagg attgcatcct tcctccaaat301 ctcactcaac ataataagtg cagaacaaca tgccaaagca ctgtatgaag cactagggac361 aaagacaagg tcaaaatcct tgtaaccaaa tttaatggta ttgtaatgca gtgttaacac421 aggacagtaa cagaacaccc agaaccaaan cagagagggt agggataagc taaatgaact481 cgtgg<210>19<211>611<212>DNA<213>Homo sapiens<400>191 tttttttttt caacttaacc atactttatt tgactgcatc ttatttagac agcttgcaca61 tttcaaatcc cattaaatct ctctccaaaa ggatatggga caatagaaga aaattatgca121 gcaatatcca gtcaaacagc agtccaatat tttgagatac acgtgatatt ttatagatac181 tcaactcata tctgacgtca atataattta tggttatgtt tttacaaacc tgaaagccct241 gtaacattac agcactcatt ttagtgatgt catggtgcac tgaaaagtaa ctctgatgta301 ctaccaattt gattttatct atactcctga aaagtgatga acttcatgat ttttagacac361 cataataaat atacacatcc agtcttagac atgtcagcca gtaccataac acagggcaag421 gtaaagttaa aacatgaaaa aacaaactgc tttacataga tttgtcgagc aagtgggaag481 ggtaatttgg gaaagaggca gaaaatatta taatcgattn ttgtattttc cttaatttgg541 ctggttcaac ttcataaact accggtctgg gttctgatct gtggacttgg ctaagtagtt601 catcccaatg a<210>20<211>477<212>DNA<213>Homo sapiens<400>201 cctcagaaac attttattga caacagttcc caacagagtc tttggggtct ttaagtggca61 ggtgcagcgt ccacaggcag agtgagggct cctgaggaac ctcaccccaa attccctaac121 cggccgagga cgcgacccca ggcccctctc aggtgggcat ggcagtcccg gcagcacccc181 ctctgagcag cctgctgtgg ggaagaagcc gggccgggag cctccagtcg tggtgccagc241 ccagctcatg ctccccgccc cgaggccccc agcctgtggg aagcccctgc ctgtaatgga301 cagctcgtga agacacagga acagtggtgg gggtgagggt ctaggaatga ggcagagggt361 ggctgagcac acacctgact ccctggaggg tcgcttcaaa gacatgggag gcgagggcac421 tggggaggct gggatgaaca accgactcca tgcacctcaa cgctctcatc aaagagg<210>21<211>449<212>DNA
<213>Homo sapiens<400>211 tttaggatga atgactgaat ttaatgatga actttgagaa ggatagacta gtaaattctg61 ttcttggacc tgagtacagt ctgggagaaa cataatgtag caggaggtct tatgaaagag121 agttaggcat tccagttgac tgagttcact gtgggatgtg acaagccact tcccaaatca181 gtgctgggaa catggcactg tgcttcacct gccaatcaga atgcccctgg agaattgtat241 tctcctcagg gcattgcttt aagagcacta cgtgttcagg aaacaaccag agaaaaccac301 cgtcaatgag cactgactgt aaagactgac gttaataaac cagaaaaaac tcaggaaagt361 gggtgagggt gtgacattgc cttcaaattc ctgaggggct gtcacttgag gagacattat421 tttggaaggc tccagtaagc ctatggaaa<210>22<211>516<212>DNA<213>Homo sapiens<400>221 cctttttctc tttggatggg agaagggagg atctgggaaa atcttatggt tctaagattt61 atctccatta acaacgttta gaagtaaagt acaggagtta aggatgtatg actgttctta121 acttataact ctacttataa aattctatga aaagaactgt caaaatatat aatactacgc181 agatataata ctatacagac atcatcaaat aaacataata ctatacagga ctattacaaa241 aaggctaacc aaccaaatat tttacttctt ggtatcaaaa ccagaagaaa ggggcttaga301 ttatggagag aataatgtaa gttgaacata tggacaggtt attcacataa aagacatatt361 aaatgcctac tgtgaaacag agcactagat ttcgtgaatg caaagatgag taagacgttg421 tccctgtcct caggaaactc ctgtgacaac gcagtaatac agcaaggtat aaggagcaca481 cagccggctt gcctagtagc tgtgtggccc ctctaa<210>23<211>493<212>DNA<213>Homo sapiens<400>231 aatattgaat aagtaatttt atttccatga tctatttaca ctgtacaaag actgttgaaa61 agcaccctac aagttcacag tacattacaa tatatttact ttaaaaatat attcctttat121 aaaaggttta taagaacatt ggcatgtcaa catgtgacaa aactctcaaa gcatgtcacc181 aattctgtga gagcaagaat gcatccaagt gttatcaatt tcacaatcac aagtcaccag241 ttgagtttta aatacataca tgttttaaat aaaaaagaat tctatagtgg aaagttcttg301 aggcaatact tattagttca ttaacgtatg cattttcatg gagatgtggc ataataaccc361 acaatatcaa cttaatctgt ccaaagaaac atttcaacaa gtttagcagt acagaaattc421 tggtcatttg tttctacttt ttcttctttg ctggttctcc tggctctact ttgcgctttt481 tagaagtatg ctc
<210>24<211>494<212>DNA<213>Homo sapiens<400>241 tttgcaaatc ttttatattt ccagctgttg agacagtatt tttgagggct gatgttacct61 ctagcggcga aaccagagcc agctattaag cagccagaaa gctacagtaa ttgaatacat121 gaccatttct cttttagcac gttctttgtt ctcctcttcc agaagttgta gacgtctatt181 tagtttgatt atctgtcgtc ttagtgaagc tgcatctaca acagtcaggt catctgacgt241 tccttcaatg gttgtatcta tatttgaaat gccatacaga gaatcatttc tgaccagctg301 tccattttgg cgaacagcat tttcactcat agaccgctct ctttttagtc tgccaactgc361 tcggatttct tcattttgag gggttgtagg aggtctttct aaatccagaa aatctagtgg421 tctttcactc agtgtaagta cacgaggtgg tgttttcagt gccaggggtt taaagggagt481 tgactgaata aggt<210>25<211>439<212>DNA<213>Homo sapiens<400>251 tttttttttt atatctcttt gttaagttta ttaatgtata cgtttttcaa ataaacattt61 tgaacagccc aaatcaatca aagccatgag atcacaaagt gttgcttctc gacacaacat121 tagcttgttc agaaaatcca aaagccacat ccaaagatca atgatgctac cttaaagaca181 tgagggaggc tccttaagac cctagagaca ccaaggaccc cacagggcca gtcatatgaa241 caggctcaga agccggcttg atcctcagca cctctgcaaa gccatcacca aaccagcaag301 tgacatgcgc tatgtctaca gtaaagtaga aaaggcggtc ctggcaaagc ttccggcggt361 acacagaccg aggatccgct gacagcacag cctcaatggc acgctttgct tcctctgctg421 actgaaaata tttaaatga<210>26<211>571<212>DNA<213>Homo sapiens<400>261 gcatatacat acaggtttat ttaaatgatt ggatctacac ttttggaggc tatttgaagt61 atcttatcaa aataaaacgc attttaagca agaagttaaa aaagcttttc agacccgaag121 gccatttaat acagtaaatc ttacttgggt agtttagcaa acatttttta aaacccacat181 ccaacagatt ggttagtatt agatacacag atttggtttc aaagctgaaa tacccctggt241 ggaataaatc ttctcatgaa attcagttat attatgccac aaaacaggga cacttatcta301 ttgacaactt aaatattaac tcagtgggct acctggtgat ataaatagga aaaaaacagt361 ggtcatggat taggagtgag aagactatga caccagccta ccttggactg ggaccgcaaa
421 ggatgttaaa atctatccta gaatttaaaa caggagaaat gtcaaatatt ccagtctcca481 ttcggctcaa gccatcgccc aaggaataca cccccacccc agccccacat gaggtttact541 aaccagctgc tacagatgtt actatgtcac t<210>27<211>447<212>DNA<213>Homo sapiens<400>271 taaattattt ggacattgat agctctgcaa aatacttctc ccatcccaaa ccctcaaact61 ccttccacat gtcctccata aagaaacaaa atatttatac tttgtgccca ttcaagtcta121 atttttaaaa acatttaaga ttcaaaatca aatattattt ttctttccca caacttgtta181 gtaattttaa accacgatac aacacagtta atggcatttc tttttaaaaa ttagcttatc241 agactatttt atgagaaaaa aatcaattca aggccttagt gagaatatta tattctctgt301 ttctgaaagc aaaagacatt gaaaaacccc tttgctcctc tggagtttgt gatttccaca361 taaccaacaa aaccagcaca cagattgact tcctacttaa cagtaggtat tgttttgtta421 ctttgggaca gcagctgaaa cactatc<210>28<211>165<212>DNA<213>Homo sapiens<400>281 tttttttttt tttttttatt ttgttctgtt ttatttggta gagcaagtga tcaaagtgaa61 gcttgagtga ttggcgccgt tggcctggcc atgggtagat cccagcatgt cccgcctccc121 actgcagctc cgtttctcgg tcacggatga gacaggtggc ctgag<210>29<211>529<212>DNA<213>Homo sapiens<400>291 tttttgccaa taaagaatat tcttttcttt attattatag ttcccattaa gctagcaaat61 gcttgttttg tagttcttcc tgtaaaaatt taccccaagt attaacaaag tatttgatag121 atgaaaaaat ttattaaagt tgaatcttca aacctaaatc tctacacaaa aggaaaaact181 tcactattaa ataacatgct cccaaggaga ttcttatata taaactgctc cacaaatgac241 caagtatctc tttgagataa atgaagcagt aataacccaa aattctttgc aatgtttagc301 agtatgagcc gatactacct gaagatcata tggattgtta aggggattag gacttttccg361 aggaaggcaa taaacaaatt ctccatcatc ttccaaaggt ttctttaaag taatcttctg421 aattgcaatt ttggagcttc cgtatttcaa actgtgctgc ggtaatatag tttgccaacc481 aagcttttct cgtagcctaa taatatttct tatcacatca tcatctttt
<210>30<211>811<212>DNA<213>Homo sapiens<400>301 tttttttttt tttttttttt tttggggnca tatttttgtt cactgaaagg accaaccagt61 ttcatcaaac aagctttaga gaaagagaaa ctgagtaatt catcttgtca gttacagttc121 acatatatgc acacacatac aaactggctc agcatcagtg aaacataact attcaaatac181 aaaagtataa aaaacctctt taaaaaacca atagcagcca aaacagaaca tttgtaaaca241 aaaccacaac tatcagccct gtgcttaaac acagaatctg cattcttttg aaacattaag301 tatatgcaat aaagagaata tagaccatct ttttccttaa tatacaatac ccaatatcta361 aaacaatgtc accaataata gacacaaatc ggtgttatca taaggcatgt tgaacagtct421 ttttcacagt actcaggggc atcatgttgc tgcagaggcc acactttcca gaagttttct481 cctcgctgtg atcctcgcac accgggggca ctcggaggac tggaagcact gtttgtgaaa541 gcaagccctg cacgctgaac atcttctaca tgttgctgtc tgaaatggga agatgacagt601 cgtattctgg caaaattcac aaataaagcc ctttccttga cacagctcac agccagccac661 atgtgcaagg gaagctttca gaatgtcctt gagtaaaggt gccagcagcc ctttcttgat721 cctgaccagg tcctaaggga ggacaggtgg agtcatcagt caagtgtccc ggcacctgct781 cgaactcctt taatgcactg gtagcaaacc t<210>31<211>488<212>DNA<213>Homo sapiens<400>311 tttttcacta ttcaacatgt cttcgtatta tcttccttcc tctcgtatct gatagacaaa61 gaatgatata aaattttggt tgaaaaaagc cagttgattg ggatgactag aaatgttaac121 tttttcagta aaaagctttc accgaatcac tgggcagtta gtaatgcagc ttacaaataa181 ataaagctga ggcgagaggt ttaaacagca agaaaagctg cagagcatat tgaggaaggg241 aagcagtgaa atggtggttt taatacatcc caaactggaa gcaagaaccc taacacaaag301 aggattggaa tactctacca tctgtaagga agtagctagc tacaagccac ctcacacaaa361 acacccacta aggcctttca ccgagatcct cttgttatat tctcattatg aatccccagc421 aggtgcacct caataaatag gtgtggagta tactgctgag gacctctagg tcacctaagc481 agaaactg<210>32<211>576<212>DNA<213>Homo sapiens<400>32
1 cttacatttc ttatacaaat aacagaatgc ttcattttat tcacttcaat aggacaaagt61 ccttaaagaa agactgaaaa gagctgataa tcaaaatccc aaattttatg cttatttttg121 ttttagtgct atcaattttc tgacatatta acataggcag gaaaacattc tcagtaaatt181 gagcatttga gtctacaaat gtcttgaagc actctggcaa gttacatgta tcccatgttg241 cttttggttt cccatctctt ctttgcttca aacccccatg caagtttctt cttttttcgg301 gcagcctgtg aattttcaac ctcctttttg gcttttacaa agttgtggca ggcaactgct361 ttggcaattt ttacaccaag ctctcgagta gctagctggt tgctgagttc ctcagccttc421 tcaatattcc actcctccac agcctggtct atcctctttt caaggcctga ctagaaaaaa481 aagaaaccta aaattaaggg ctggaaggaa agcaaacatt tgctagccaa gaccatgtca541 cagaattata ctgaatgtgt tacacaagta gaccat<210>33<211>1005<212>DNA<213>Homo sapiens<400>331 tctgcccgct gtgtcctggg catggagggt cagcaggtca tcctgcacct gcccctatcc61 cagaaggggc ccttctggac atgggagcct agtgcccctc gaactctgct ccaggtccta121 caggatccag ccctgaaaga cctcgtcctc acctgcccca ccctgccctg gcattccctg181 atcctgcggc cccagtatga gatccaagcc atcatgcaca tcttctcaag tcttaggatt241 gcagcaacac gctcggctgc ccaaacccaa ggcgaagacc ttgccagagt tcatcaagga301 tggctccagt acgtacagca agattcctgc ccacaggaag ggccacaggc ccgctaagcc361 ccaaaggcag gatctagatg atgatgaaca tgattatgaa gaaatacttg agcaatttca421 gaaaaccatc taagtgctgg aggaaccacg cttcctaact gctgcttctc agggaatccg481 acaccagcca accattttaa gcctctaaaa gacctcgggc aagtctcaca gaaactgagc541 tgcagacggg gagtagcttt gtggaaactg atttgatgga cactgcacca gcttccttca601 ggttctagat tcttgctact tagggcgggc tggtttggac ctaacatctc gcacgtgact661 ccctcagcct cagagccttg ggatgcagag cagctggcag ggttcctctc aatcctgcaa721 ccccagctgt cccaccggtg gatgcagagg ggaatccgag gccatcaacc ttggtgacag781 cagcgcagtg ccaatgctga tcacactgca tgggagattt tgttaacgtc tgccaccccc841 actctcaccc ccaagctcta agcccccggg aggcctggac tgtcttcctc atctctgtag901 caccaagcct gatagatctg tatatggtaa acaggggttt aaccacatgt ggttaacatg961 gattaatgtg ggaatttggc ttcaagaaca caaccttagg acctt<210>34<211>483<212>DNA<213>Homo sapiens<400>341 ggcacgaggg gctcaaagag aagtataaga ctaggtcttt atatttgtaa gttacttaca61 ctctagatat caaacgaaat gtaactcaca tgaagtgctt caatgttgaa tagtgaggaa121 cgcacagcgg aaacatattc ttgcagccca tcagatcatc cacagttaca cggaaagcat
181 ggttcaaaag aaattaactg agggagagca agatgaggcc tcaaatgctg ccatgttact241 tgagaaagaa actcaaaatg agaacaacag ctaagattgt gatttttttg caaaagaagc301 tgtacgaatc atcataaata attccaatag tctgcacttc tcccaaggtg tgcactgctc361 ataaacttgc cagtgtgtga ttcatgagaa aggagattta ttttccttaa acatccaata421 atttaaaatg attttcaata ataataatca aatgaaaaaa aaaaaaaaaa aaaaaaaaaa481 aaa<210>35<211>644<212>DNA<213>Homo sapiens<400>351 gcgcgggcgg ctggggaatg gctgctgcca tggtgccggg gcgcagcgag agctgggagc61 gcggggagcc tggccgcccg gccctgtact tctgcgggag cattcgcggc ggacgcgagg121 acaggacgct gtacgagcgg atcgtgtctc ggctgcggcg attcgggaca gtgctcaccg181 agcacgtggc ggccgccgag ctgggcgcgc gcggggaaga ggctgctggg ggtgacaggc241 tcatccatga gcaggacctg gagtggctgc agcaggcgga cgtggtcgtg gcagaagtga301 cacagccatc cttgggtgta ggctatgagc tgggccgggc cgtggccttt aacaagcgga361 tcctgtgcct gttccgcccg cagtctggcc gcgtgctttc ggccatgatc cggggagcag421 cagatggctc tcggttccag gtgtgggact atgaggaggg agaggtggag gccctgctgg481 atcgatactt cgaggctgat cctccagggc aggtggctgc ctcccctgac ccaaccactt541 gacttaatct cactttctta aattcttcta ttctcagaca ctgctctagt accattcctt601 cctcttagcc ccaggagcaa attaaaaggt acagttaaaa tcct<210>36<211>1434<212>DNA<213>Homo sapiens<400>361 cctcgtgcca tgagcattta gaaatggaga ccacttctgg tgaattgtca gatgtttcca61 atgatcccac cacatctgtc tgtgtagcta gtgccccaac aaaagaagca attgacaata121 ccctgcttga acacaaagaa agaagtgaga atataaatct taatgaaaag caaattcctt181 ttcaggtgcc taatattgaa aggaacttta gacagtcaca gcctaaatcc tatctttgcc241 attatgaact ggctgaggcc cttccacaag gaaagatgaa tgagacacca actgagtggc301 tgcgttataa ttcaggaatc cttaacacac aaccaccatt accattcaaa gaagcacatg361 tcagtggtca tacttttgta acagctgagc aaatcctggc tccattagct ttaccagagc421 aagcattatt gatcccacta gaaaaccatg acaaattcaa aaatgtacca tgtgaggtct481 accagcacat tctgcagcca aacatgctgg ccaacaaggt taaatttacc tttcctccag541 ctgccctccc accccctagc acacctctgc agcctttgcc tttgcagcag tccttatgtt601 ctacctctgt aaccactatc catcacactg ttttgcagca gcacgctgca gctgctgcag661 ctgcagctgc agccgcagct gcaggaacct ttaaagtgct tcagccacac caacagtttc721 tttcccaaat cccagctctc accagaacct cattacctca gctctcagta ggaccagtag
781 gaccgaggct ttgtcctggg aaccagccaa cttttgttgc tcctcctcag atgccaatca841 ttccagcttc cgttcttcat cctagccatc tggctttccc atctttaccc catgcactct901 ttccttcact gctttcccca caccctactg tcatcccttt gcaacctctc ttctagtcat961 caccataatg ggaaaaaaat actcttgtga aaactattgc tatatgcgtt aagtgttcat1021 ctatgtgggt acatggctat ttaactggtg gaaataaact ggccgataca tggcgtcatt1081 ggtttgaaat catttactgt aagtgcaatg atgcaaataa atccctaagt ttctgatata1141 taatattatt aaagcactga atagtttgaa aatcaataca atatatgcta tatattaaaa1201 tgatgtctta agagtatgta taatgtacat aaaatatatt tatagtactc taatttatgt1261 tgtaaagtat gctcccttgg tttttcttaa tctttgtgta tttgcaccta tttaatgttt1321 agacaaagct gatggcacta tgttttgtat catgttcctt gaaactgtaa attcagtgaa1401 aaatatctct tgcaataaat ttttgttaac tatttaaaaa aaaaaaaaaa aaaa<210>37<211>1640<212>DNA<213>Homo sapiens<400>371 gggctgcgtg cggcgggaat catggctgct cgcagagctc tgcacttcgt attcaaagtg61 ggaaaccgct tccagacggc gcgtttctat cgggacgtcc tggggatgaa ggttctgcgg121 catgaggaat ttgaagaagg ctgcaaagct gcctgtaatg ggccttatga tgggaaatgg181 agtaaaacaa tggtgggatt tgggcctgag gatgatcatt ttgtcgcaga actgacttac241 aattatggcg tcggagacta caagcttggc aatgacttta tgggaatcac gctcgcttct301 agccaggctg tcagcaacgc caggaagctg gagtggccac tgacggaagt tgcagaaggt361 gtttttgaaa ccgaggcccc gggaggatat aagttctatt tgcagaatcg cagtctgcct421 cagtcagatc ctgtattaaa agtaactcta gcagtgtctg atcttcaaaa gtccttgaac481 tactggtgta atctactggg aatgaaaatt tatgaaaaag atgaagaaaa gcaaagggct541 ttgctgggct atgctgataa ccagtgtaag ctggagctac agggcgtcaa gggtggggtg601 gaccatgcag cagcttttgg aagaattgcc ttctcttgcc cccagaaaga gttgccagac661 ttagaagact tgatgaaaag ggagaaccag aagattctga ctcccctggt gagcctggac721 accccaggga aagcaacagt acaggtggtc attctggccg accctgacgg acatgaaatt781 tgctttgtcg gggatgaagc atttcgagaa ctttctaaga tggatccaga gggaagcaaa841 ttgttggatg atgcaatggc agcagataaa agtgacgagt ggtttgccaa acacaataaa901 cccaaagctt caggttaacg gaagacatga tgcagagcaa gcctctgtga ttcctgccca961 gcacctgtga ggcctgacgt gtcagttccc aataaatgct cttctgattt gtttcccgta1021 caggcaagga ggcttgggta gtgcagattt gtgtatttca atctttgaaa gctctgatgt1081 aatttagaaa tgaaatccaa tcatgagtcc aggtagagaa cgcctgctgt aatctacact1141 gttgctggga ctgcgcattc tgtatataac tgtgttggat gagtgacaga tgattgtcca1201 gactaggaca gcggcatgaa catgactttg gttgggattg cggatagtta gggttacctc1261 tgaatcgtgt agcttttatg agagcagctg tgcaagtgaa tccacattaa tgccttgtcg1321 tggtgccatt cccagcgcct gacgatacgc tcttctattg tcttattctg gcaggttttg1401 acgttttaaa ttttttaaag aaattttatt ccttggacca aaaggtttgg ttaaccaccc1441 ccctcttact tgctttcaca ttttgagtgt ccagaggaaa cagaaaggaa tgagtgtgtg1501 acgttgctgc acgcctgact ctgtgcgagc ttctttctgt gtatatattt tgttttattt
1561 ttttccgtgt atatttttaa tcccgacaga acatcatgtg agatttcttt aaaatggatt1621 aaacgatttc ttcagccg<210>38<211>1428<212>DNA<213>Homo sapiens<400>381 agcggcctcc ggtgtgggat ggccgcggag ccgggcggag ctggcttgcg gctcccgggg61 ccggctctcc ggccggagac atggcccggg ggcccggccc gctaggcagg cctcgccccg121 atacggtcgc catgcccaag agaggaaagc gactcaagtt ccgggcccac gacgcctgct181 ccggccgagt gaccgtggcg gattacgcca actcggatcc ggcggtcgtg aggtctggac241 gagtcaagaa agccgtagcc aacgctgttc agcaggaagt aaaatctctt tgtggcttgg301 aagcctctca ggttcctgca gaggaagctc tttctggggc tggtgagccc tgtgacatca361 tcgacagcag tgatgagatg gatgcccagg aggaaagcat ccatgagaga actgtctcca421 gaaaaaagaa aagcaagaga cacaaagaac tggacggggc tggaggagaa gagtatccca481 tggatatttg gctattgctg gcctcctata tccgtcctga ggacattgtg aatttttccc541 tgatttgtaa gaatgcctgg actgtcactt gcactgctgc cttttggacc aggttgtacc601 gaaggcacta cacgctggat gcttccctgc ctttgcgtct gcgaccagag tcaatggaga661 agctgcgctg tctccgggct tgtgtgatcc gatctctgta ccatatgtat gagccatttg721 ctgctcgaat ctccaagaat ccagccattc cagaaagcac ccccagcaca ttaaagaatt781 ccaaatgctt acttttctgg tgcagaaaga ttgttgggaa cagacaggaa ccaatgtggg841 aattcaactt caagttcaaa aaacagtccc ctaggttaaa gagcaagtgt acaggaggat901 tgcagcctcc cgttcagtac gaagatgttc ataccaatcc agaccaggac tgctgcctac961 tgcaggtcac caccctcaat ttcatcttta ttccgattgt catgggaatg atatttactc1021 tgtttactat caatgtgagc acggacatgc ggcatcatcg agtgagactg gtgttccaag1081 attcccctgt ccatggtggt cggaaactgc gcagtgaaca gggtgtgcaa gtcatcctgg1141 acccagtgca cagcgttcgg ctctttgact ggtggcatcc tcagtaccca ttctccctga1201 gagcgtagtt actgcttccc atcccttggg ggcagcctcg agtgtagtcc attagtaatc1261 agattccagt ttggacaggg tggctggatt gtatatctcg ttagtaatgt acatgctctt1321 caggttctag ggctcctgtt aggggaggga gaaatgttga atcaagaggg aaaacaacta1401 ctatgattta taaacatatt ttaatgtaaa aaaaaaaaaa aaaaaaaa<210>39<211>855<212>DNA<213>Homo sapiens<400>391 ggcacgaggg ggaagctgaa gcagttcgat gcctacccca agactttgga ggacttccgg61 gtcaagacct gcgggggcgc caccgtgacc attgtcagtg gccttctcat gctgctatgg121 tgctgaggca gaagatatca agtgctgtaa cacctgtgaa gatgtgcggg aggcatatcg181 ccgtagaggc tgggccttca agaacccaga tactattgag cagtgccggc gagagggctt
241 cagccagaag atgcaggagc agaagaatga aggctgccag gtgtatggct tcttggaagt301 caataaggtg gccggaaact tccactttgc ccctgggaag agcttccagc agtcccatgt361 gcacgtccat gacttgcaga gctttggcct tgacaacatc aacatgaccc actacatcca421 gcacctgtca tttggggagg actatccagg cattgtgaac cccctggacc acaccaatgt481 cactgcgccc caagcctcca tgatgttcca gtactttgtg aaggtggtgc ccactgtgta541 catgaaggtg gacggagagg tactgaggac aaatcagttc tctgtgacca gacatgagaa601 ggttgccaat gggctgttgg gcgaccaagg ccttcccgga gtcttcgctc atctaccact661 cagcacgagc catccagaag aaaattgatc tagggaagac aacgtagtca ccctcggtgc721 ttcctctgtc tcctctttct ccctggcctg tggttgtccc ccagcctctg ccaccctcca781 cctcctcggt cagccccagc cccaggttga taaatctatt gattgattgt gatagtaaaa841 aaaaaaaaaa aaaaa<210>40<211>587<212>DNA<213>Homo sapiens<400>401 gggatgagtg gaggatggga tgagtggagg atgggatgag tggcgttggc tgcagatttg61 tggtgcgttc tgagccgtct gtcctgcgcc aagatgcttc aaagtattat taaaaacata121 tggatcccca tgaagcccta ctacaccaaa gtttaccagg agatttggat aggaatgggg181 ctgatgggct tcatcgttta taaaatccgg gctgctgata aaagaagtaa ggctttgaaa241 gcttcagcgc ctgctcctgg tcatcactaa ccagatttac ttggagtaca tgtgaaagaa301 aacgtcagtc tgcctgtaaa tttcagcaag ccgtgttaga tggggagcgt ggaacgtcac361 tgtacacttg tataagtacc gtttacttca tggcatgaat aaatggatct gtgagatgca421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa<210>41<211>437<212>DNA<213>Homo sapiens<400>411 ggattcactg ttacttcctc tttattcctg cagtgaaaac aatattttct tatgtcagat61 ttctctcaat ctcttagtca atgaagacct gaactacaac aatatagagt cccattttaa121 attcaaacaa agatatttag gtttgacaac agactcatgg taatgtcatt taaaaagatg181 gatccaatga acacagacaa acatcaaagt tttcaccatg gcacaaacct ctgccacaat241 cactcataat aattcaactg ccttcatatt tggcagtaac gtcatgcaag tgaatttgct301 gatgattagc aaaatggcaa aatgaaagtc aaggtcactt aatcaagaat tacagcaaca361 aagaaattct aggaatgcag gtcaaggcaa gaggctgcag ctggcctgat aatgatacca421 cttgcctaat gttcatt
<210>42<211>710<212>DNA<213>Homo sapiens<400>421 tttttttttt tttttattct aaaatacaac gtttattaaa aaaacatgcg atcttgccaa61 taggccaatt ccatgagaac aaaagatttt ttttcccacc tacaagtctt tttagagaaa121 caaacggaac tatttccaga tgaggcgggg tgtctgggag gggctgtggg tggtctgggg181 ctggtgactg gtgaggttgg gtggccctct gggggtccgg gaggcacttt ccgttttgct241 tctgttggag atgttcccgt cggatgctga agaacaagcc tgccggctgg gccactccga301 cctgggctgt ggcacccgtg tggccctcgg ccctcctggc ctgccgttcc ccagggccgt361 caccgccgat agcggtagcg cttgaagtga aaccacagct ggaagatgcc gttggcaaac421 tggcagcgga agccgtggcg gtgcgagtat tcccactcgc gggtgacgat cttgaaagcg481 atgtcctcgt agggcggccc cgcgtggaag cgcacgatgg cgaaatcctt gttgtcggcg541 caggccttca agaagtactc tggcgtggag cgcttgtcga taagtctggg gtaaagaagt601 gaacttgtat ccatggacga acttgggcgg aggttggtaa aatcgaaatg cgtctggttg661 accttggtcc actcgaaacc cgtgtgcacc ccgttgaaag agcgccgctt<210>43<211>388<212>DNA<213>Homo sapiens<400>431 tttttttttt ttaaaaatga tggtttatta ttaaaatgat cttacaatgt caacatcaat61 gttaataaaa atatataata ggctgaattc atcaatgata gaataagttg taattcactt121 ggaggttcca tctttcaaag taagcctttc atagataaat gaaaatcctt tattttgtag181 aattttaaag attgttaaag gctgggtcaa ggcaaagcca cctctattag aaggggaaag241 aaaagcaaga tgaaacaaaa tatgttatca tacatatcgc gtgtgctatg agcatctttc301 tactcctgcc agattgaaaa ttctaggttt caacattctt caggatttaa caagtcaaaa361 taaaagcaga attcaaatct agacagat<210>44<211>433<212>DNA<213>Homo sapiens<400>441 agaaagctgt agcaggagct ccagaaaatt tgcacgtgca gcccccagca gttcaacaga61 ctgcacgtct ttggaaaagt ctttgaagat atttgtaaca gttctttgat atttggtgat121 ctcttgaaaa aagttaagga tgaatatgaa ctctacatgg caacgctcct ggagtcccag181 cctgcagcac agtacgaggc tcttctggct caactcaagg cgctggggca gaggccggtg241 aagacggcgg acatggatct cgccagggaa gagctgagga tgctcgtgac agccacaaaa
301 gcagccctgg agcagaatga tagactcaga agtgaactgg agatggaggt ggcattgctg361 cagtctgcan aggaacgatc agaatcatct gagacacata taattgatga aaaccgactt421 actcttactg aga<210>45<211>811<212>DNA<213>Homo sapiens<400>451 tttttttttt ttttttttcc aaagagtaaa tattttaatt ttttcttcaa gagtattctt61 attttcatat tatttaaata gtcacattaa caaggtacat ttccacaaaa atatttgtac121 agcatcagta ttcttattca catccataat tgccataatt aggaaaaatt aggaaactta181 gcaaagacag tcaatacaca tgttctttta cttgtggtga gataaattca atgtctaaaa241 taagtcagga tggatcaaca taaaagctga actagacacc cctaccaaat ttctcaatga301 aaaagcaaat agccctgggt gaccagcaag ttatacaaac aatgcaagac aaatccaact361 agaaatcgga agctgccatt tcgtatacac aaaatacaag tgaaacagcg aagtaaacat421 atttttagag ggaggtgctg ctacccggac attagagata tactgggcca gctggaaata481 acattcacaa tctcttgaaa tgcagcagat ggcactctgg ggcttcctat gaagcaacat541 gcttgaaatc aagggccaac aattgttgta ggaaagcaaa atatacctct aacacctacg601 tttaccaaaa aagctgacat ctcaaactct gagttgttga gactcacatt tctcatcccc661 aaagaagcct attacgggag tgcgttggat gctttttgta tctctgatag gccggcccct721 atagtggggg gaatacttct gaataaaaac ctgggctgac ttgcacctgg cctttaacgt781 tattncaggg gggcgtgggc ctaactgaac c<210>46<211>400<212>DNA<213>Homo sapiens<400>461 tttttttttt tttttttttt atttacccac gattcttctt tatttggctg ccaaatatag61 gggcacgtta gtattgcggt cagcttaata ttaagtagaa ggcattaaag gctaaaacag121 gggtgcaaaa agctgtaggt aaatctcttc tatgctggct gttagcaaag cactgggatg181 ctatataatt gcagcaaact ttttttttgg ttctgctgca ctgcagctca taaagggagc241 actcattctg gaaaaaaaaa aaaaagttaa atctagcaac tggttagcac cagcattcca301 aaaaacttca gcagcctgtg ttttcagcct ctgctccaac ttcattgctc tttggaatca361 agagcttcat gttcaggaat agccaccatt ggatgactca<210>47<211>590<212>DNA<213>Homo sapiens
<400>471 ttcataagta aaatcaattt tattagtaag gctctttcca catgtccaaa gcaaacaatt61 aatgaattcc agggaaaacc acctatatac aatgaaatta gaagtatctc aggatgacaa121 aaaatataca aatatgtaca cccataatca ccgccgcctc cgaaaacctt ttttcttccg181 acctttctga ggtatcttct tccgcttctt aataggattc tgatcctcat caagatgaac241 agttccccct gtcaaagcac tgatgtcact gaagtgtgta agggaatgaa gttgtgagct301 catgacattt gctcgttttc gccgtccttt ctctcgctta ctgacgctca aacgcaccat361 catgctctcc tcatagttaa tcctgtgttg gcctcctgac tctggcggga acatngggat421 gccgagcatc acggatttcc tctggagcat ctgagtactg ctcctttaag tcacgaatga481 cagagctgct caatgcccgt cntcttggct cgtctagacg cttcttctcc ccggtcaggt541 tcttgttcat cataattgac ttgaacccag cgtggaggaa catatttctt<210>48<211>504<212>DNA<213>Homo sapiens<400>481 gcggccgcac catgagcgac atccgccgcc caccccgccc tgcccacgca ccccggccac61 accttcatct ccggcgtgac ctttcccttc aggcccatcc gctaggctgg cccgtgtgtg121 ccttctgcgc tctcgctgga cgaagccttt cgagatggaa ggggtggccg gactcccaga181 agagaacctc ggggaagggg tcgggcagcc cctccccgcc ggcagaaccg tcttggtgtc241 acggagtcca ggtgcttccc acccggtcgc attctttgac atgcagattg gatggtggag301 ggaagagtcc agcctctgcc gagagcctgc tgcgtgcatt tttaaaagat gccgatcctg361 ggagcctctg ttctctgcgc atttcagaca cagcctgtgt ggcgaggagt gtgacggcag421 gagccacggg tgcaagcccg tgtgtctggc ctctntcctc gtgaagacga tgtgtccccg481 ccagaaaaag tgggctcctt ctgc<210>49<211>456<212>DNA<213>Homo sapiens<400>491 gcctgggggg gaaaaaaccc acaaatgtct ttccccagtt tcctccagta acacagatgg61 aaatagtagt ttatacttgg gatggtgctc ggggaaatag tgtctcagaa ccaattactg121 aaatgaccag aatggatata aaatgtggct gcataatttc ctaagtccca tttctcaggg181 taaaaatggt tcagaccatc caaatgcttc tccgaagaaa ttacacaagc gagatggaaa241 aagaaagtat tggtttctca gcgctggtaa taattaggac ttgaatgagt ggtgataaac301 ccatttacag acaggaagtg tgaaggcctg aatggccagg agatttgcta agtgatgaca361 agatcaagat caggcctctg gcctctgagc ccagctccct aagcctagtg aactttgaat421 gacacctgat tttttgcccc atccagcacc catttn<210>50
<211>566<212>DNA<213>Homo sapiens<400>501 tttgctctct acagaagcct ttactgggga ggggtcagag ttcatggatc accgggaggt61 gcgagtagag tgttctgagg acactgctgg ggcgggggcg gtggcgggag gccatggctt121 gggcagactg cccaaaagtc cagcttctta ggtcagagct ctgctgccca tcctgcggtg181 gtcttgccag ggaatgccca ggcatcctgg cttcacagag cctccctctg ggggccccca241 tgggcttgct gctgtccatc tgtctatgtg gaccccagag gccagcagag gggccagtcc301 tccttggcag gtccctctgg cttggctgct tttcctggga ctccccaaaa agccagtcca361 gggggtccac agtatcagcc atgggcagtg tgggctgctg gccactgaaa cgtgcggtga421 tgtctcgcag ggacacagca aggcggccct tccggcaggc ctgggctggc ctgtggggtg481 gcctgtgttg gccctgngcc gtggccttgt ccagcacaga ggtgggctgt gccagtcgag541 atatagacct cacgtagtca tccagg<210>51<211>515<212>DNA<213>Homo sapiens<400>511 acttttatta gtctttttat tcttctttta ttgatcacct ctgaagttca gtagcacact61 gcataatttt ttgcattagc atgctgttgt ttctacctaa aatgaccggg gtccttgcct121 tctgcttccc atagagattg tcccaactcc ataggtccat agtcacaaat gaggacagct181 ttacatgtaa catgaatcta aaagaaaaaa acaccaccct taattaagag gcaaggaagc241 tatgacagat ccctcccaaa tgcagtaata ggtcagctat tactatacca acctccagag301 cttcacacgc atccttccct tagcctaaga ttatacttca ttttacaatt tcagagatcc361 aaccatctct gaaacctgtc tgaaactagc ccacatgcca tataccagat tacaacattt421 aggccagctt cactattact tttcctcctt ttacgaccac caagaccagt gttcagatcc481 gatgggaaag ggatcaggag gattaagctg atcaa<210>52<211>469<212>DNA<213>Homo sapiens<400>521 tttgacattt tatgttcaaa tatatttgct gcctcaccct acagggcccc tccctaagga61 ggaccacact cctgcccagg atgtccggca cagccctgtg acttggttgg ccggtggaat121 atgagtgggt aggatctgtg cccttccctt gtcagcagag gctcagaagc tccctgaggg181 tccactgttt caccatttcc ctttgtccag aggccagggc atcctgacag agtcagcctt241 ttgcgtaagg tcactggctt ggttgatgta ccggtcacgc tccttttcca gatgaaagat301 gatctttctc tgcttctgag cctcatcctt gtagttctgg atttctccct ccaggttcct
361 cttggcttgt tcatggaacc ttaccaaagt ttgtctgctc ctggggcgca ttgaccgcct421 taagcatgtt cttatttagt atgtcccttt ctcttagaag ctcgtccat<210>53<211>585<212>DNA<213>Homo sapiens<400>531 gtataaaatt taaattttat gatcatgtct ggaccttttt acacttaaca caatggaaat61 gttcatattc atttttttcc ccaaaggaaa aatttctgaa tcatcactat gttgcacagt121 agaatagaaa tatgaattca ggtacttagt caagtttcta aagaatgcaa ataacagaca181 tgatttaact tccaaaatct gaagtcatca acatgatttt gtagtactct atctcaggtt241 ttacctaaaa attcgttatt attcagataa agatagctcc ctaatcacat gtttttaaaa301 agcctctgcc ccacgacaac ttcctgagat ctgtgaagaa actgcagtct tgtgatcaga361 ctccatatag gactggcagt tttgtaatcg tagggaaaaa acggtgactg attttacgtg421 tcaatcagtt gtagtctgct gatctgatta gaagccttga caaatgcctg gtggcgattg481 caaataactg ccaagcatat agggatgata cagtagccca ctgttttggg atcagctctt541 gtacaagagt ataggaacaa aaacatctgt aagtaaggta ccaga<210>54<211>676<212>DNA<213>Homo sapiens<400>541 agtgagacaa tttattttta ttgcctaaac tgcagagcat tctctacaca gcgtaaagga61 gaagtggggc agctgcagga catgtagaga aaccaaaccg ggaaatctta caaggagttg121 aaaagattaa tgtcccaacc tgatgagatt acggccttga gacaataagg tggagcaaca181 gaacaatggg aaagaggact gggagaggaa tctaagaacc agatggcttc agctctctag241 aggagctgag cctattcaca gtgcctaacc tggaagcctg tgaggaacag gcctcaggcc301 cttgccatga cctacttagg ctacaatttc ctttaaggca gcccctagct ctgggaagga361 atactggtag ccagtggccc agtgttcgcc gtgggatcac cttctggccc tccagcagca421 tgatggcacg ctgtcgccca aagacagctt gcaccacagc gctggggaga gggatgaagg481 ctcggcggcc cagggcagca ccgaaggtct gggcaaactc agcattagtg gcggaggatg541 gagccactcc attcaggacc ccgtgcacgt ggtttgcttc aagggcatgg gtcaggattc601 ctgccaggtc cccgatgtgt atccaggggg agaattgggg gcctgagccg atggggcccc661 ccaggcccag gcgaaa<210>55<211>455<212>DNA<213>Homo sapiens
<400>551 ggtccgggtt agctttattg gagcctccta gcaggccagg tgtttcaggc accagtagtg61 ctgcaggcac gggggcctgt ggtgtggcca gctgcaggtg atctcggtgg tggactccga121 agtgtactcc agagaggtcc cggaatggca ggggaccagc tcgggcactg aggcgctgct181 ggcaggctgt ttcgacgatt ccttctggcg ctgtccgctg gtcttggggg ctgcgtcctc241 cggcttgcca gaggacttgc tggggggctg cgtggggatg ttgctgctgc tgggagactg301 gaccatgggt gggaccgggc tggaggtgac gttgggtggc tgcaggggcc ccgaggacgg361 gagggctgac tgggaggggc cggaggaagt accaggcggc tgggtgtgtt gccgagacca421 gtgggagctg ccctgtgccc gacgctggcc ctgng
权利要求
1.一类在人类肝脏中表达的表达序列标签的序列，其特征在于，它包括(a)SEQ ID No.1～SEQ ID No.55所示的序列；(b)SEQ ID No.1～SEQ ID No.55所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.55所示的序列中每条序列有至少70％同源性的序列；(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的表达序列标签的序列，其特征在于，所述序列包括具有SEQID No.1～SEQ ID No.55所示的序列。
3.一种探针分子，其特征在于，所述的探针分子含有权利要求1中所述的序列中约8-100个连续的核苷酸。
全文摘要
本发明公开了一类在人类肝脏中表达的表达序列标签的序列。利用本发明的在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物中发挥重要作用。
文档编号C07H21/04GK1955294SQ20051003082
公开日2007年5月2日 申请日期2005年10月28日 优先权日2005年10月28日
发明者黄健, 韩泽广 申请人:上海人类基因组研究中心