S1p的用途的制作方法

专利名称：S1p的用途的制作方法
技术领域：
本发明涉及S1P(鞘氨醇-1-磷酸盐)的用途。本发明的其它方面涉及用于筛选药物的方法和用于治疗疼痛的方法。
疼痛是一种复杂的主观感觉，它反映真实的或潜在的组织破坏及对其做出的情感反应。急性疼痛是显示潜在损害或真实损害的生理信号。慢性疼痛或者是躯体性(器官性)的或者是心理性的。疼痛常常伴随或继发植物性神经病征，并由此导致抑郁。
躯体性的疼痛可能源于伤害、炎症或者是神经性的。伤害性疼痛被认为是躯体或内脏的疼痛敏感神经纤维正在激活。神经性疼痛源于神经系统功能障碍，它被认为是通过周围神经系统、中枢神经系统(CNS)或两者中感觉异常过程维持的。(对于疼痛机制的综述，见例如Scholz和Woolf，2002；Julius和Basbaum，2001；Woolf和Mannion，1999；Wood，J.D.，2000；Woolf和Salter，2000)。
慢性疼痛导致了患者个人的痛苦及社会的巨额经济花费。现有的药理学疼痛疗法在疗效和安全性上尚不令人满意。
迄今为止，治疗疼痛的镇痛药主要为两大类非阿片样物质镇痛药(主要是对乙酰氨基酚和NSAID(非甾体抗炎药))及阿片样物质(麻醉药)激动剂(“阿片样物质”是在中枢神经系统内结合特异阿片受体并产生激动剂作用的天然或人工合成物质的通称)。不幸地是，阿片样物质和非阿片样物质两类镇痛药都有几种有害的副作用。阿片样物质最严重的副作用是可能抑制呼吸系统，并且经过长期治疗后可能出现成瘾性(Schaible H.G.，VanegasH.，2000)。另一方面，非阿片样物质中NASID是主要的一类，它可引起多种胃肠道并发症如溃疡和出血，另外可导致肾损害(Schaible H.G.，Vanegas H.，2000)。据估计，在美国每年大约有16,000名患者死于因常规应用NSAID而引起的严重胃肠道并发症。
鉴于目前本领域疼痛治疗水平的严重缺陷，寻找新一类疼痛调节药物成为迫切要求。特别是根据疼痛神经生物学认识的飞快发展与尚不能满足临床需要的无本领域治疗现状缺点的有效治疗之间的巨大差距，应努力发现用于新类镇痛药物的靶点。因此，本发明的目标是提供用于开发和提供新类疼痛调节药物的一种新的手段。
通过使用S1P或者其功能片段或衍生物制备调节疼痛的药物化合物，该目标得以解决。
本发明是基于发明者首次证实的发现，即S1P参与伤害处理及其具有减轻疼痛的能力。术语关于S1P的功能性或术语S1P功能指S1P与至少一种其受体相互作用并且优选地活化受体、或降低细胞内cAMP水平或介导PAM(Myc相关蛋白质(Protein Associated with Myc))从内质网到细胞膜易位的能力；更优选地指其增强PAM活性(即PAM与AC相互作用和/或降低AC活性和/或减轻疼痛的能力)和/或抑制AC活性的能力甚至更优选地指其减轻疼痛的能力。术语关于S1P受体的功能性或者术语S1P受体功能指S1P受体与S1P相互作用的能力，更加明确地指介导由S1P相互作用启动的受体典型信号的能力以及更加明确地指影响由S1P相互作用启动的疼痛处理的能力。
S1P片段可以是比根据

图15的野生型分子更小的任何片段。PAM片段可以是比相应野生型短的任何片段多肽或多核苷酸。S1P受体片段可以是比相应野生型短的任何片段多肽或者多核苷酸。
S1P或S1P片段的衍生物可以是具有S1P功能的分子的任意修饰或任何其它类型修饰，例如能够导致分子稳定或调节其特异靶向特定细胞或者利于其进入细胞或被细胞摄取的化学或生物修饰；一种已知的修饰是S1P的羟基化或甲基化。适宜的修饰或添加物对于确保或促进其靶向所需位置及进入细胞是有用的。另一方面，局部应用(如使用合适导管的脊柱内应用)等能够保证其靶向脊髓。其他有用的添加物包括用于其稳定等作用的盐(生理可耐受有机或无机盐，见例如Remington′s Pharmaceutical Sciences，1418页，1985)、缓冲液等。
由于S1P可以通过特异受体被细胞内化，所以它可以外部应用并且随后被特异性内化。例如，通过克隆并在在所需细胞内表达S1P受体可以实现S1P靶向的调节。应用本领域已知的适当基因启动子/增强子能够保证细胞类型特异表达。
本发明基于发明者的研究，即首次证明S1P在脊髓和背根神经节(DRG)内感觉机制中的令人惊奇的关联。
S1P(鞘氨醇-1-磷酸盐)是鞘氨醇的磷酸化衍生物，鞘氨醇是所有鞘脂的结构主链。该细胞外(朝向血清)鞘脂通过结合五种特异G蛋白偶联受体(命名为S1P1-S1P5)之一来调节多种细胞过程，其中五种G蛋白偶联受体在不同的组织差异表达，每一种调节特异的细胞行为。(对于综述，见例如Payne等，2002；Spiegel和Milstien，2000)。细胞外S1P的已知功能包括如细胞迁移、细胞存活或血管发生的调节。除了它的细胞外作用，还已知它作为细胞内信使起作用(对于综述，见例如Payne等，2002；Spiegel和Milstien，2000)。然而迄今尚不知它在伤害性过程中的作用。
PAM(Myc相关蛋白质)是一个分子量为510kDa的巨大蛋白质。本领域已知PAM的蛋白质序列、基因组序列和编码核苷酸序列，例如可从NCBI(National Centre for Biotechnology Information；National Library ofMedicine，Building 38A，Bethesda，MD 20894，USA；www.ncbi.nhm.nih.gov)数据库的登录号AAC39928(编码序列；SEQ IDNo.1)、AF075587(蛋白质序列；SEQ ID No.2)公开获得。人PAM定位于染色体13q22，其基因组序列可从NT_024524.11公开获得(起始24679861位；终止24962245位；SEQ ID No.3)。备选地，蛋白质序列和编码序列可从KIAA0916的蛋白质登录号NP_055872(蛋白质序列)和NM_015057(编码序列)公开获得。
对于大鼠PAM，可公开获得下列EST克隆编码序列AW921303(对应于人cDNA的bp960-1394；SEQ ID No.4)AW918711(对应于人cDNA的bp8188-8632；SEQ ID No.5)BQ201485(对应于人cDNA的bp8966-9633；SEQ ID No.6)
BE112881(对应于人cDNA的bp10311-10830；SEQ ID No.7)AW441131(对应于人cDNA的bp13569-14152；SEQ ID No.8)BF409872(对应于人cDNA的bp13569-14807；SEQ ID No.9)。
PAM最初是通过其与Myc N末端的转录激活结构域的特异相互作用能力而被鉴定出来的(Guo Q.等，1998)。最近发现PAM是AC活性的有力抑制剂(Scholich K.，Pierre S.，Patel T.B.Protein associated with myc(PAM)is a potent inhibitor of adenylyl cyclase.J.Biol.Chem.2001，12月14日；276(50)47583-9)。但是，迄今还没有证据证实它在伤害过程和感觉中的作用。
当然，PAM被认为在突触前生长调节中发挥着作用已知PAMmRNA在特定解剖部位包括海马、齿状回和小脑中高表达。在成年大鼠和小鼠脑组织中，PAM和Myc的表达局限于小脑中的成熟浦肯野细胞和海马中的粒细胞及锥体细胞(Ruppert C.等，1986；Yang H.等，2002)。然而，在这些细胞类型中没有一个已知参与疼痛过程和感觉。
已经表明果蝇的PAM同系物(highwire)和秀丽线虫(C.elegans)的PAM同系物(rpm-1)在突触前末梢组织形成(Zhen等，2000)、突触生长调节(Wan等，2000)、突触发生、轴突生长和靶向(Schaefer等，2000)中起着决定性作用。这些发现导致了以下假设highwire、rpm-1和它们的哺乳动物同系物PAM充当突触生长的负调节蛋白(Chang等，2000；Jin Y.，2002)。因此，在这些结构的主要突触发生时期，在小脑、海马和齿状回中PAM表达急剧升高(Yang等，2002)。
在啮齿类的脑发育过程中，PAM表达在出生后即刻开启，在开始的两周内表达上调并且其后在成年期PAM表达保持增高的水平(Yang等，2002)。到目前为止，对于PAM在脊髓和DRG中的表达和调节以及它在感觉机制和调节疼痛中的作用一无所知。
先前，已证实人类PAM是环AMP(cAMP)信号的有效调节物并且在纳摩尔浓度能够抑制几种腺苷酸环化酶(AC；E.C.4.6.1.1)异型体的酶活性(Scholich等，2001)。
普遍存在的环AMP(cAMP)第二信使系统是将细胞外刺激转变为细胞内信号和反应的多种不同信号转导机制之一。在具有细胞外刺激时，G蛋白偶联受体(GPCR)通过三聚体GTP结合调节蛋白(G蛋白)调节细胞膜结合酶或离子通道。通过GPCR调节其活性的酶之一是一种产生cAMP的酶-腺苷酸环化酶(AC)。因此，细胞外刺激的进入影响了细胞内介质cAMP的细胞内浓度。cAMP水平的升高通过刺激蛋白激酶A(PKA)影响细胞，其中蛋白激酶A磷酸化特异的细胞内靶蛋白并且因此改变它们的活性。
每种细胞类型都有特征性的GPCR组、由这些GPCR调节的酶、特异腺苷酸环化酶亚型以及靶蛋白，它们与更多非特异或广泛存在的参与者(如普遍存在的cAMP)一起作用，使每个细胞对细胞外信号做出各自独特的反应。例如，已知环AMP(cAMP)第二信使在调节突触可塑性中发挥主要作用(Bailey等，1996；Xia等，1997；Brandon等，1997)；另一方面，它参与代谢过程和细胞增殖。因此，普遍存在的cAMP信使系统和它的不同成分会根据不同组织和细胞类型的不同特化而发生变化。
目前为止，在本领域已知作为S1P受体起作用的5种不同GPCR，命名为S1P1-S1P5(对于综述，见例如Spiegel，S.和Milstien，S.，2000)。不同S1P受体的蛋白质和编码多核苷酸序列是本领域公知的并且可以从例如NCBI(National Centre for Biotechnology Information；National Library ofMedicine，Building 38A，Bethesda，MD 20894，USA；www.ncbi.nhm.nih.gov)数据库的登录号NM_001400(SEQ ID No.32；人类内皮分化鞘脂G蛋白偶联受体1 mRNA(EDG1/S1P1)的核苷酸序列)；NP_001391(SEQ ID No.31，人类内皮分化鞘脂G蛋白偶联受体(EDG1/S1P1)蛋白质序列)；NM_004230(SEQ ID No.34，人类内皮分化鞘脂G蛋白偶联受体5(EDG5/S1P2)mRNA的核苷酸序列)；NP_004221(SEQ ID No.33，人类内皮分化鞘脂G蛋白偶联受体5(EDG5/S1P2)蛋白质序列)；NM_005226(SEQ ID No.36，人类内皮分化鞘脂G蛋白偶联受体3(EDG3/S1P3)mRNA核苷酸序列)；NP005217(SEQ ID No.35，人类内皮分化鞘脂G蛋白偶联受体3(EDG3/S1P3)的蛋白质序列)；NM003775(SEQID No.38，人类内皮分化鞘脂G蛋白偶联受体6(EDG6/S1P4)mRNA的核苷酸序列)；CAA04118(SEQ ID No.37，人类内皮分化鞘脂G蛋白偶联受体6(EDG6/S1P4)的蛋白质序列)；NM_030760(SEQ ID No.40，人类内皮分化鞘脂G蛋白偶联受体8(EDG8/S1P5)mRNA的核苷酸序列)；NP_110387(SEQ ID No.39，人类内皮分化鞘脂G蛋白偶联受体8(EDG8/S1P5)的蛋白质序列)公开得到。
本发明的另一方面涉及作为药物(优选作为预防或治疗疼痛的药物)使用的S1P或者其功能片段或衍生物。
本发明的另一方面涉及S1P或者其功能片段或衍生物在调节疼痛中的用途。这种调节优选地是减轻、预防或总体抑制。
此外，本发明包括S1P或者其功能片段或衍生物在鉴定调节疼痛的化合物中的用途。调节化合物优选地是具有、模拟或增强S1P活性的化合物。最优选地是他们具有预防、减轻或消除疼痛的能力。
可通过例如化合物的以下能力鉴定化合物a)模拟、恢复、活化或增强S1P功能的能力(即与至少一种其受体或其片段相互作用的能力并且优选活化受体的能力，或降低细胞内cAMP水平或介导PAM从内质网至细胞膜易位的能力；更优选地是指其增强PAM活性和/或抑制AC活性的能力并且甚至更加优选地是减轻疼痛的能力)或者模拟、恢复、活化或增强PAM功能的能力(即降低细胞内cAMP水平的能力、与其它因子如AC(特别是AC)相互作用的能力、抑制AC的能力或者其降低疼痛感觉的能力)，或者b)增加血清S1P水平的能力(例如，通过激活或增强S1P产生或减少其细胞外降解)，或者c)增强至少一种S1P受体表达的能力(即通过其转录的激活、转录物的稳定性、翻译或其翻译后加工；通过其翻译后修饰的调节或者通过其稳定性的活化或其降解的抑制等)，或者d)与负责S1P产生或降解的酶相互作用的能力。
本发明的另一方面涉及预防或减轻疼痛的方法，包括向个体施用足够量的S1P或者其功能片段或衍生物。
应该以允许S1P靶向作用位点(DRG或脊髓)的方式适当地进行施用，例如通过向血流(例如静脉应用或口服应用)全身施用S1P衍生物或制剂或者通过局部(例如脊柱内)应用S1P或者其片段或衍生物。
本发明的另一方面涉及筛选用于调节和/或预防疼痛药物的方法，包括步骤a准备包含PAM或者其功能片段或衍生物但不包含S1P的样品，b准备包含PAM或者其功能片段或衍生物并包含S1P的第二个样品，c将至少第一个样品与化合物接触，d测定样品中PAM活性，e确定化合物模拟S1P功能的能力。
本方法还可包含另外步骤，其中将细胞与S1P接触而不是与化合物接触，而且其中将根据以上c)和d)测定的PAM活性与S1P存在时的PAM活性比较。
另一实例涉及一种方法，包括步骤下a)准备包含表达S1P受体或者其功能片段或衍生物的细胞的两个样品，和b)将一个样品与化合物接触，和c)测定两个样品中的受体活性。
根据本发明另一方面的方法，包括步骤a)准备包含表达S1P受体或者其功能片段或衍生物的细胞的两个样品，和b)将样品与S1P接触，和c)将一个样品与化合物接触，和d)测定两个样品中的受体活性或者S1P与受体的相互作用。
根据本发明的不同方面，PAM或者S1P受体可以来自任意可获得序列，这些序列允许用于它们的特殊目的。优选人PAM或S1P受体。
为了本发明的不同方面，如果PAM或S1P受体是分离的多肽或寡核苷酸或多核苷酸，则它也是优选的。在本发明不同方面的上下文中，分离的意思是指至少从天然来源或重组分子(当然，它也可以是纯化的或部分纯化的)中部分纯化。
测定法是监测生物学过程的任何类型的分析方法。为了应用于药物筛选，测定法需要是可重复性的，并且优选地还是可批量的和确切的。这种测定优选地适用于对具有调节(优选降低)和/或预防疼痛能力的化学物质的高通量筛选。高通量筛选主要包括对大约500,000种化合物的某些能力进行筛选。测定法的类型取决于例如所使用分子类型(或者是多肽或者是多核苷酸)以及“读取法”，即S1P、PAM或S1P受体活性被确定的方式(见下文)。
此类测定法的不同类型通常是本领域当前水平已知的，并且可从商品供应商获得。适宜测定法包括放射性同位素或荧光测定法，例如用于测定标记成分与非标记成分相互作用(例如PAM或其片段可以被标记并且可以监测它们与AC的相互作用)的荧光偏振测定法(例如，由Panvera、Perkin-Elmer life sciences(例如LANCE)或者Packard BioScience(例如HTRF或ALPHAscreenTM)商业性提供的那些)。
简单的生物化学测定法适于例如检测潜在的药物化合物与受体或功能性受体片段或衍生物之间的相互作用。更精细的测定法还能够检测化合物是否能够活化特定受体并因此模拟S1P的活性。
更多的实例包括基于细胞的测定法，其中细胞系稳定表达(可诱导性表达或不可诱导性表达；位于染色体上或附加体上)或瞬时表达目的重组蛋白。这些测定法包括如报道基因测定法，其中根据报道基因的酶活性测定特定启动子的调节或信号转导级联中成分的信号转导途径，报道基因的表达处于所述特定启动子的控制下。对于这种测定方法，必须构建包含处于所定义启动子控制下的报道基因的重组细胞系，其中启动子可以是其本身正在被研究的启动子或者是被正处于研究中的信号级联放大所调节的启动子。适宜的报道酶通常是本领域技术水平已知的并且包括萤火虫萤光素酶、海肾萤光素酶(例如，通过Packard reagents商业获得)和β半乳糖苷酶。适宜的细胞系取决于分析目的并且包含易于转染和易于培养的大部分细胞系，例如HeLA、COS、CHO、NIH-3T3等。
测定细胞内离子水平的测定法包括例如FLIPR(荧光成像板读取仪，从Molecular Devices商业可得)测定法，其中氩激光光源和致冷CCD照像机的组合允许平行测量384孔板中细胞(如神经元细胞或其他细胞如重组或天然表达某些离子通道的细胞)内短暂离子信号(如Ca2+等)。FLIPR测定法允许使用荧光染料诸如Fluo-3、Fluo4监测细胞内的钙，或者使用BCECF或BCPCF或特定FLIPR测定试剂盒监测细胞内的pH值，或者使用DiBAC或特定FLIPR测定试剂盒检测膜电位的变化，或者检测膜极化。为了检测其它的细胞内离子如锌或钠，可使用本领域技术水平已知的其它染料。其它类型测定法和其它类型的读取法通常是本领域技术人员已知的。
为了测定cAMP水平，如AlphaScreen、荧光极化或HTRF技术是合适的。
为了测定离子通道活性(其控制着如细胞内离子浓度，因此能够用于测定细胞内离子浓度)，可使用膜电位敏感测定法和染料例如基于FLIPR技术的DiBAC或Molecular Devices公司的膜电位测定试剂盒；使用FLIPR技术的线粒体膜极化测定JC-1染料；用于测定细胞内钙离子浓度的离子敏感染料如Fluo-3、Fluo-4或Molecular Devices公司的钙测定试剂盒；用于测定细胞内钠的来自Molecular Probes的钠敏感染料；用于测定细胞内钾浓度的基于膜片钳或基于原子吸附光谱的铷离子外流的测定法等。检测细胞内某些变化和状态的更多的自动设备和分析法是本领域技术人员已知的并且包括例如ACUMEN bioscience公司的Acumen检测器(基于荧光的激光扫描读取器，它允许对适当标记的物体进行三维重建)。
为了测定GPCR活性如cAMP测定，通过Packard Bioscience的AlphaScreenTMcAMP检测系统、Ca2+动员检测法或报道基因检测法是合适的。
PAM多肽优选地是包含SEQ ID No 2的序列的多肽或者是由SEQ IDNo 2的序列组成的多肽，或者是由包含SEQ ID No 1或3的序列的多核苷酸编码的多肽或者是由SEQ ID No 1或3的序列组成的多核苷酸编码的多肽。S1P受体多肽优选地是包含SEQ ID No.31、33、35、37或39的氨基酸序列之一的多肽或者是由SEQ ID No.31、33、35、37或39的氨基酸序列之一组成的多肽，或者是包含SEQ ID No.32、34、36、38或40的核苷酸序列之一的多核苷酸编码的多肽或者是由SEQ ID No.32、34、36、38或40的核苷酸序列之一组成的多核苷酸编码的多肽，或者是由这些mRNA序列所包含的编码序列编码的多肽。
PAM多核苷酸优选地是包含SEQ ID No 1或3的序列的多核苷酸或者是由SEQ ID No 1或3的序列组成的多核苷酸，或者是包含能够在严格条件下与以上多核苷酸杂交的序列的多核苷酸或者是由能够在严格条件下与以上多核苷酸杂交的序列组成的多核苷酸。S1P受体多核苷酸优选地是包含SEQ ID NO.32、34、36、38或40的序列的多核苷酸，或者是由SEQID NO.32、34、36、38或40的序列组成的多核苷酸，或者是包含或对应于SEQ ID No.32的244-1392位、SEQ ID No.34的1-1137位、SEQ IDNo.36的1-1062位、SEQ ID No.38的23-1177位或SEQ ID No.40的10-1206位的多核苷酸，或者包含能够在严格条件下与上述多核苷酸之一杂交的序列或者由能够在严格条件下与上述多核苷酸之一杂交的序列组成。
严格性指影响两个单链核酸分子杂交或退火特异性的反应条件。严格性和因此而来的反应特异性尤其依赖于反应所使用的温度和缓冲液条件例如通过升高反应温度和/或降低反应缓冲液的离子强度可以提高严格性和因此而来的特异性。低严格条件(及因此而来的低反应和杂交特异性)例如在2×SSC溶液中室温杂交。高严格条件包括例如在0.1×SSC和0.1％SDS溶液中于68℃进行杂交反应。
在本发明不同方面中的严格条件下的杂交优选的被理解为
1)在50mM Tris pH7.5、1M NaCl、1％SDS、10％硫酸葡聚糖、0.5mg/ml变性鲑精或鲱精DNA的条件下将标记探针与所分析核酸样品于65℃杂交过夜，或者在寡核苷酸探针的情况下在低于寡核苷酸与样品的双链体退火温度或解链温度(在以下把退火温度和解链温度理解为同义的)5℃的温度下杂交。
2)室温下用2×SSC洗涤10分钟。
3)在65℃(对于寡核苷酸的情况下温度低于退火温度5℃)用1×SSC/0.1％SDS洗涤30分钟。
4)在65℃(对于寡核苷酸的情况下温度低于退火温度5℃)用0.1×SSC/0.1％SDS洗涤30分钟。
用作杂交探针的寡核苷酸是多核苷酸并且优选具有15-30(优选20)个核苷酸长度的DNA片段。根据公式Tm＝2×(A+T的数量)+4×(G+C的数量)℃确定退火温度。
为了制备2×SSC或0.1×SSC(或任何其它类型SSC稀释液)，例如可将20×SSC溶液相应稀释。20×SSC由3M NaCl/0.3M柠檬酸钠×2H2O组成。
在进行杂交反应之前，如果希望的话可以在电泳分离之后(然后进行Southern印迹(DNA)或Northern印迹(RNA))或不进行电泳分离(然后进行狭线印迹或斑点印迹)，将多核苷酸转移到合适的膜上如尼龙膜或硝酸纤维素膜。使用合适的标记探针进行杂交。适宜的标记技术如放射性标记或使用荧光染料标记。探针是单链的多聚核糖核酸或多聚脱氧核糖核酸，它可以是天然的单链或者通过双链变性产生的单链。该探针通过碱基配对结合DNA或RNA样品(其同样也是单链状态的)。
PAM片段优选地是包含于上述序列ID No.1、2或3的片段并且衍生物优选地衍生自上述序列ID No.1、2或3或其片段。S1P受体片段优选地是以上序列SEQ ID No.31-40包含的片段，更优选地是包含SEQ ID No.32的244-1392位、SEQ ID No.34的1-1137位、SEQ ID No.36的1-1062位、SEQ ID No.38的23-1177或SEQ ID No.40的10-1206位的片段或者是由SEQ ID No.32的244-1392位、SEQ ID No.34的1-1137位、SEQ ID No.36的1-1062位、SEQ ID No.38的23-1177或SEQ ID No.40的10-1206位的片段组成，并且衍生物优选地来自这些序列。
功能片段或其衍生物优选地能够抑制腺苷酸环化酶(AC)活性，更优选地能够抑制腺苷酸环化酶I型、V型或VI型(关于S1P受体，最优选地是当通过S1P的结合或模仿S1P的分子的结合被活化时能够抑制AC活性)。
根据本发明不同方面的优选实施方案，PAM的功能片段或衍生物包含人类PAM序列(优选根据SEQ ID No.2的人类PAM序列)中氨基酸400-1400，优选地446-1062、499-1065或1028-1231，更优选地1000-1300，并且甚至更加优选地1000-1100并且甚至更加优选地1028-1065，或者PAM的功能片段或衍生物由人类PAM序列(优选根据SEQ ID No.2的人类PAM序列)中氨基酸400-1400，优选地446-1062、499-1065或1028-1231，更优选地1000-1300，并且甚至更加优选地1000-1100并且甚至更加优选地1028-1065组成，或者如果它们由各个多核苷酸片段编码的话，则特别是由包含于SEQ ID No.2或3的序列编码。
如果功能片段或其衍生物是多核苷酸，如果它们包含编码上面多肽片段的多核苷酸或由编码上面多肽片段的多核苷酸组成，则是优选的。更加具体而言，优选地是它们包含人类PAM cds的1482-3332位(编码氨基酸446-1062位)或1641-3341位(编码核苷酸498-1066位)或3228-3839位(编码氨基酸1038-1231位)或者是由人类PAM cds的1482-3332位(编码氨基酸446-1062位)或1641-3341位(编码核苷酸498-1066位)或3228-3839位(编码氨基酸1038-1231位)组成。如果用于衍生片段的人PAM cds具有SEQ IDNo.2的序列，则甚至是更加优选的。
根据用于鉴定疼痛调节化合物的本发明方法的一个优选实施方案，使用了表达S1P受体和/或PAM的细胞，优选地是表达重组S1P受体和/或PAM的细胞。
细胞可以是任何类型细胞，例如真核或原核单细胞生物(诸如细菌如大肠杆菌(E.coli)或酵母如粟酒裂殖酵母(S pombe)或酿酒酵母(S.cerevisae)或来源于多细胞生物的细胞系(诸如HeLa、COS、NIH-3T3、CHO等)，其中优选哺乳动物细胞系。
根据另一个优选的实施方案，使用了经过修饰的细胞，其中修饰的细胞与未修饰的细胞相比具有较低的S1P受体活性。通过这种方法可以检测被检测具有调节(优选减少)和/或预防疼痛的能力的化学化合物是否能够增强或恢复降低了的或总体去除了的S1P受体活性。
修饰可以是导致S1P受体活性和/或PAM活性(即它们降低细胞内cAMP水平、PAM易位的能力、抑制AC的能力或降低疼痛感觉的能力)、S1P受体或PAM转录本稳定状态水平(即通过抑制S1P受体或PAM转录或转录本稳定性)或S1P受体或PAM蛋白质稳定状态水平(即通过S1P受体或PAM的翻译或其翻译后加工过程的失活；通过翻译后修饰的调节或通过其稳定性的失活或通过其降解的增加)降低的任何类修饰(稳定的修饰或暂时的修饰，优选稳定的修饰)。这可以通过例如使用S1P受体或PAM的显性失活突变体、反义寡核苷酸、RNAi构建体、通过产生功能性或基因组S1P受体或PAM的敲除(例如它可以是可诱导的)或本领域技术水平已知的其它适宜技术实现。对于以上技术的综述，见例如Currentprotocols in Molecular biology(2000)J.G.Seidman，第23章，附录52，John Wiley and Sons，Inc；Gene Targetinga practical approach(1995)，A.L.Joyner编著，IRL Press；Genetic Manipulation of Receptor Expressionand Function，2000；Antisense Therapeutics，1996；Scherr等，2003。
依据优选的实施方案，可使用PAM敲除细胞。用于产生敲除的适宜细胞系是本领域众所周知的并且包括例如Current Protocols in MolecularBiology(2000)J.G.Seidman，第23章，附录52，John Wiley and Sons，Inc；或Gene Targetinga practical approach.(1995)，A.L.Joyner编著，IRL Press。
可直接测定S1P活性，例如通过其(或其片段和衍生物)与其至少一种受体或受体的功能片段相互作用的能力或者其引起PAM向细胞膜易位的能力；也可以间接测定S1P活性，例如通过其(或其片段和衍生物)降低细胞内cAMP水平、调节神经元内离子浓度、抑制AC功能的能力或者调节(特别是降低)疼痛感觉的能力。用于测量上述参数的适宜技术是本领域众所周知的(还可见以上内容)如cAMP水平可通过HTRF或ALPHAscreenTM测定，离子浓度可通过膜片钳或合适的染料检测，疼痛感觉可通过福尔马林测试或者机械或热痛觉增敏测试或热平板测试等检测。S1P与其受体的相互作用可通过例如cAMP测定、Ca2+动员或报道基因分析确定。
本发明的另一方面涉及鉴定调节疼痛化合物的方法，包括a)选择能够调节或模拟S1P活性的化合物作为测试化合物，和b)向受试者施用所述测试化合物以确定疼痛是否得到调节。
受试者可以是具有感知疼痛的任何受试者，优选地是哺乳动物，可以是非人哺乳动物或人(即在病人研究中使用人)。
调节优选是预防、减轻或消除疼痛。依据本发明的一个优选实施方案，化合物是S1P受体激动剂(对于综述见例如Mandala等，Science 2002)，更优选地是FTY 720(2-氨基-2-(4-辛基苯基)乙基)丙烷-1，3-二醇，见图17)或其功能衍生物或类似物(类似物是本领域已知的，见例如Brinkmann等，JBC，2002)(即具有以上指出的调节疼痛能力的衍生物或类似物)，优选磷酸化衍生物(见Mandala等，Science，2002)。化合物或其衍生物或类似物的生理可接受盐也是合适的。
根据本发明的另一个方面，包含向个体施用足够量的具有结合和激活至少一种S1P受体和/或激活PAM功能的能力的药用化合物的能够减轻或预防疼痛的方法处于本申请的范围之内。此化合物的一个适宜实例是S1P受体激动剂(对于综述见例如Mandala等，Science 2002)并且优选地是FTY720或上面定义的功能衍生物或类似物(优选其磷酸化衍生物)或者化合物或其功能衍生物或类似物的生理可接受盐。
以下通过实施例或图来更加详细阐明本发明。但是，实施例并不意味着限制本发明的范围。
实施例PAM表达模式及PAM和S1P功能的研究1.材料S1P购自Tocris(Ellisville，MO)，抗Hsp70抗体和抗钙联接蛋白抗体购自BD Transduction Labs(Bedford，MA)。抗活化ERK1/2抗体购自Promega(Madison，WI)。百日咳毒素、U0126、U73122和Wortmannin购自Tocris(Ellisville，MO)，RO31-223、BAPTA-AM和GF109203X购自Sigma(St.Louis，MO)。
2.动物切片制备野生型Sprague Dawley大鼠购自Charles River Wiga GmbH(Sulzfeld，Germany)。实验前动物自由摄食和饮水。在气候和光控制的室内饲养它们(24+0.5℃)。在每一场合只使用动物一次。在所有试验中，遵守有意识动物的研究伦理学指南，并且方法得到当地伦理委员会的认可。处死后，成年大鼠用含4％多聚甲醛的0.1M磷酸缓冲盐(PBS，pH7.2)灌注固定一小时。组织在水平台上用冷冻切片机切成14-16μm厚度切片。将切片粘在Superfrost Plus Slides载玻片上(Fisher Scientific Co.，Pittsburgh，PA)并储存在-80℃备用。
3.核糖核酸探针制备如以前所述(Yang等，2002)制备核糖核酸探针。在使用Hind III(反义链)或BamHI(有义链)分别将质粒线性化后，使用T7和T3聚合酶得到大鼠PAM反义核糖核酸探针和正义核糖核酸探针(见Yang等，2002)。按照制造者的建议，在[35S]UTP-αS(ICN，Irvine CA)、线性PAM cDNA、NTP存在下，于37℃1小时进行体外转录(Promega，Madison，WI)。使用RNA探针纯化试剂盒(Pequlab，Erlangen，Germany)纯化RNA转录本。
4.原位杂交按照以前所描述(Yang等，2002)进行原位杂交切片用4％多聚甲醛(0.1M磷酸缓冲盐溶液(pH7.2))固定，用0.25％乙酸酐和0.1M三乙醇胺预处理，用0.2×SSC洗涤并且用浓度递增的系列乙醇脱水。切片用预杂交液(50％去离子甲酰胺，0.6M氯化钠，10mM Tris-HCl(pH 7.6)，50mM EDTA，0.025％焦磷酸钠，0.02％菲可，0.02％BSA，0.02％聚乙烯吡咯烷酮，10mMDTT和热变性的异源核酸(0.005％酵母tRNA，X型，0.05％酵母总RNA，I型，0.05％鲑鱼精巢DNA，III型))室温杂交2小时；在杂交液(2.5×106cpm/片)(50％去离子甲酰胺和50％杂交缓冲液，50％杂交缓冲液含有0.6M氯化钠，10mM Tris-HCl(pH7.6)，50mM EDTA，0.025％焦磷酸钠，0.02％菲可，0.02％BSA，0.02％聚乙烯吡咯烷酮，热变性的异源核酸(0.005％酵母tRNA，X型，0.005％酵母总RNA，I型，0.05％鲑鱼精巢DNA，III型)，100mM DTT，0.0005％聚腺苷酸，10％硫酸葡聚糖)中与核糖核酸探针于50℃杂交过夜。切片用2×、1×、0.5×SSC室温冲洗。在20μg/ml RNA酶A(Sigma，St.Louis，MO)消化后，用1×RNA酶缓冲液、2×、1×、0.5×SSC室温冲洗切片，并在0.1×SSC中45℃冲洗过夜。切片用浓度递增的系列乙醇脱水后，在-80℃用Kodak Biomax MR胶片(Kodak，Rochester，NY)曝光3-7天。
5.抗体产生和免疫荧光染色用分别包含对应于SEQ ID No.1的人PAM的135-153位和4601-4614位氨基酸残基的多肽从兔中产生抗血清，抗血清是通过公司商业性产生的(Bio Trend，Cologne，Germany)。按照标准方法由德国的Bio Trend，Cologne公司商业性生产抗血清。为了检测PAM在脊髓和DRG切片中的分布，用0.1％Triton X-100透化处理切片5分钟。切片用3％BSA(用PBS配制)封闭1小时，然后用抗PAM抗血清(1∶50稀释)孵育1小时。其后用FITC标记的山羊抗兔抗体(用含3％BSA的PBS配制)孵育。然后用PBS洗涤切片并使用fluoromountTM封片。
6.逆转录PCR(RT-PCR)通过异硫氰酸胍/酚/氯仿提取法(Chomczynski和Sacchi 1987)从大鼠脊髓和DRG分离总RNA。将2μg总RNA与0.6μM的每种oligo(dT)引物退火，并使用逆转录酶(Promega，Madison，WI)于37℃逆转录30分钟。然后立即将cDNA用于扩增。用于扩增大鼠GADPH的寡核苷酸引物为5′-GAAGGGTGGGGCCAAAAG-3′(正义链；SEQ ID No.10)和5′-GGATGCAGGGATGATGTTCT-3′(反义链；SEQ ID No.11；Trajkovic等，2000)。用于扩增AC异型体的寡核苷酸引物选自Xu等的公开(Xu等，2001)。用于大鼠PAM的引物是5′-GGTGGTGAAGCTCGCTGTGATGCT-3′(正义链；SEQ ID No.12)和5′-CGTGTGAGCATTTCTGCACACTCC-3′(反义链；SEQ ID No.13)。PCR产物对应于人PAM cDNA的第13692-14064位核苷酸。相应的大鼠序列来自EST克隆AW441131(SEQ ID No.8)。对于半定量PCR，可使用SAWDAY DNA聚合酶(Peqlab，Erlangen，Germany)。在95℃5分钟的起始变性步骤后，进行30个循环(95℃，1分钟；55℃，30秒；72℃，10秒)，接着在72℃最终延伸10分钟。使用TaqManTM系统和试剂(AppliedBiosystems，Weiterstadt，Germany)按照制造商的说明书进行定量PCR。
7.全长PAM的纯化使用先前发表的方法(Scholich等，2001)并加以修改来进行PAM的纯化。简言之，HeLa细胞在含10％胎牛血清和1％青霉素/链霉素的DMEM培养基中生长。用1×PBS、1mM EDTA收获40个150mm培养皿内的汇合细胞，400×g离心5分钟沉淀细胞。用含有125mM NaCl，20μg/ml抑酶肽，20μg/ml亮抑酶肽，1mM苯甲脒，5μg/ml大豆胰蛋白酶抑制剂的TED缓冲液(50mM Tris-HCl，pH8.0，1mM EDTA，1mM DTT)重新悬浮细胞，用5秒的超声处理2次裂解细胞。将细胞匀浆液于4℃，27000×g离心30分钟，然后将上清液装载于Q-Sepharose XK16柱(Amersham，Pharmacia，Piscataway，NJ)并且根据制造商的说明书用TED中150-350mM NaCl的梯度洗脱。根据标准方法通过Western印迹分析各级分；将阳性的级分混合并调整NaCl浓度至1M。然后将蛋白质装载于Phenyl-Sepharose XK16柱(Amersham，Pharmacia，Piscataway，NJ)并且按照制造商的说明书用TED中的300mM NaCl洗涤。流出液和洗涤级分中含有PAM。将它们混合在一起并使用Centricon 50(Amicon，Beverly，MA)按照制造商的说明书将缓冲液更换成上面提到的含有100mM NaCl的TED缓冲液。然后将蛋白质装载于MonoS 5/5FPLC柱(Amersham，Pharmacia，Piscataway，NJ)，并按照制造商的说明书用装载缓冲液(含有100mM NaCl的TED)洗涤。收集流出液并装载于Mono Q5/5FPLC柱(Amersham，Pharmacia，Piscataway，NJ)。用TED中150-400mM的NaCl梯度将蛋白质洗脱下来。将阳性级分混合并使用Centricon 50(Amicon，Beverly，MA)将缓冲液更换为50mM Tris-HCl，pH8.0，1mM DTT，-80℃储存。储存的PAM在3周内使用。
8.重组体Gsα的表达和纯化如Graziano等1991所描述，表达和纯化了带有六组氨酰标签的Gsα的组成型活化Q213L突变体(Gsα*)。为了确保Gsα*的最大活性，在用于AC活性测定之前，在MgCl2(25mM)存在下用1μM GTPγS与G蛋白孵育30分钟。
9.腺苷酸环化酶活性测定(AC活性测定)在25mM Hepes，pH7.4，1mM EDTA中裂解脊髓并按照Kassis和Fishman描述的方法制备细胞膜。将等分试样储存在-80℃备用。如先前所描述(Patel等，2002)，AC活性分析在以下条件下进行，即在100μM MgCl2存在的条件下在100μl体积内室温进行15分钟。Gsα*(80nM)或毛喉素(forskolin)(100μM)用于刺激膜中(10μg蛋白质)的AC酶活性。
10.PAM反义和正义寡核苷酸的脊柱递送用氯胺酮(60mg/kg，腹腔注射)和咪达唑仑(0.5-1mg/kg，腹腔注射)麻醉大鼠。切开从第13胸椎(Th13)至第3腰椎(L3)的脊柱外皮肤。去除第2-3腰椎周围的肌肉组织。去除第3腰椎的棘突，第2腰椎行椎板切除术。然后，将聚乙烯导管(ID 0.28mm，OD 0.61mm)插入硬膜下隙以致于导管顶部可到达第9-10胸椎。将导管用氰基丙烯酸酯胶固定，置于颈部区域表面并缝合皮肤。
11.PAM寡核苷酸的注入寡脱氧核苷酸(ODN)序列选自以下大鼠PAM序列。正义链5′-GACTGGTTTAGCAATGGC-3′(SEQ ID No.14)，反义链5′-GCCATTGCTAAACCAGTC-3′(SEQ ID No.15)，含有3个突变的反义ODN((3M-as；突变用下划线标明)5′-GCAATTGCTAAATCAGTA-3′(SEQ ID No.16)。术后3天，将大鼠置于一个“自由活动系统”(CMA，Stockholm，Sweden)，使用微量注射泵(CMA，Stockholm，Sweden)将反义寡核苷酸(n＝5)或正义寡核苷酸(n＝5)(2.5mg/ml人工脑脊液)以0.05-0.1μl/分钟的流速经导管注入100小时。
12.福尔马林测试在停止注入15分钟内进行福尔马林测试。将50μl 5％甲醛溶液经皮下注射(s.c.)到一只后爪的背侧面。注射福尔马林后立即以1分钟的间隔计算收缩次数，直至60分钟。每间隔5分钟的收缩次数总结为每分钟的平均收缩次数。为了比较组间的伤害性行为，将1小时观察时间内的两个阶段的收缩次数的总和进行Studentst检验，α设置为0.05。
福尔马林测试结束后，处死大鼠，切除腰脊髓和背根神经节(DRG)，在液氮中迅速冷冻并储存于-80℃直至进一步分析。为了测定PAM的表达，可以使用以上抗PAM抗体对脊髓切片进行免疫组织化学分析。
13.酵母聚糖引起的炎症为了诱导炎症，将2.5mg酵母聚糖A(Sigma，St.Louis，MO)悬浮于30μl 0.03M磷酸缓冲盐(PBS，pH7.5)中，皮下注射入右后爪中部足底区域。已知此种足底酵母聚糖注射可诱导可靠的热和机械的疼觉增敏大鼠模型(Meller和Gebhart，1997)。酵母聚糖注射24-96小时后，在深度异氟醚麻醉下通过心脏穿刺杀死大鼠。切除腰脊髓和背根神经节(DRG)，在液氮中迅速冷冻并储存于-80℃直至进一步分析。
14.免疫荧光染色为了检测PAM在HeLa细胞中的分布，将细胞在含10％FBS和1％青霉素/链霉素的DMEM培养液(Gibco，Karlsruhe，Germany)中生长于玻璃盖玻片上。为了观察PAM在HeLa细胞内的易位，将细胞进行血清饥饿过夜，然后用10％血清处理2小时。无论在所指出的任何情况下，将细胞在不同浓度抑制剂存在下预孵育30分钟。细胞用4％多聚甲醛(Sigma，Taufkirchen，Germany)(PBS配制)固定10分钟，然后用0.1％Triton X-100透化处理5分钟。盖玻片用3％BSA(PBS配制)封闭1小时，然后用抗PAM抗体(1∶50稀释)孵育1小时。其后用FITC标记的山羊抗兔抗体(用含3％BSA的PBS配制)孵育。然后用PBS洗涤细胞并封片。为了分析可使用共焦(BioRad，Hercules，CA)和常规荧光显微镜(Nikon，Duesseldorf，Germany)。
15.cAMP积累超声处理脊髓样品并于4℃，18,000×g离心20分钟。上清液用于cAMP测定。通过cAMP检测试剂盒(Assay Design Inc，Ann Arbor MI)按照制造商的说明书检测细胞内cAMP积累。
16.反义寡脱氧核苷酸如先前所公开(Scholich等，2001)选择寡脱氧核苷酸序列。正义链5′-CTGTTCATGCCGGTT-3′，反义链5′-AACCGGCATGAACAG-3′，和包含三个突变的反义ODN(3M-as；突变用下划线标明)5′-AATCCGTATGAACAC-3′。将HeLa细胞铺于35mm培养皿中(300,000个细胞)并在含10％FBS和1％青霉素/链霉素的DMEM培养基(Gibco，Karlsruhe，Germany)中培养24小时。通过使用Tfx20(Promega，Madison，WI)并按照制造商的说明书在1ml无血清培养液进行转染，将ODN(每个3μM)导入细胞。两小时后，加入1ml含10％FBS的DMEM培养液(Gibco，Karlsruhe，Germany)。然后将细胞孵育6小时。再次更换为无血清DMEM培养液(Gibco，Karlsruhe，Germany)，并在用10％血清或500M S1P处理之前用无血清DMEM培养液(Gibco，Karlsruhe，Germany)孵育16小时。向细胞中加入1×Laemmli缓冲液用于Western印迹或者如上所述收集细胞用于AC活性测定。
17.活化PAM的血清因子的纯化将188ml胎牛血清(Gibco，Karlsruhe，Germany)调整至0.3M NaCl的终浓度。然后将血清装载于Phenyl-Sepharose15/10柱(Amersham，Pharmacia，Piscataway，NJ)。将该柱以及下面的柱子洗脱之后，收集流出液和所有洗脱级分并分析它们诱导PAM在HeLa细胞内易位的能力。然后将流出液装载于Q-Sepharose15/10柱(Amersham，Pharmacia，Piscataway，NJ)。用含400mM NaCl的TE(50mM Tris/Cl pH7.4，0.5mMEDTA)洗涤柱并用含1M NaCl的TE洗脱。将洗脱液装载于Superdex200pg凝胶过滤柱(Amersham，Pharmacia，Piscataway，NJ)上。按照制造商的说明书用TE洗脱蛋白质，并分析级分诱导PAM在HeLa细胞内易位的能力。将阳性级分混合，装载于MonoQ 5/5FPLC柱(Amersham，Pharmacia，Piscataway，NJ)并用含400mM NaCl的TE洗涤。用TED中的400-1000mM的NaCl梯度洗脱蛋白质。将阳性级分混合，并按照制造商的说明书装载于Superdex 50pg凝胶过滤柱(Amersham，Pharmacia，Piscataway，NJ)。根据制造商的说明书用TE洗脱蛋白质，并分析级分诱导PAM在HeLa细胞内易位的能力。将阳性级分混合并储藏于-80℃并在两周内用于质谱分析或生化分析。如Caligan等所述通过phthaldialdehyd标记和随后HPLC分离检测S1P。
18.证明疼痛过程中牵涉PAM的结果本发明者使用RT-PCR、免疫组织化学和原位杂交的上述实验首次证明PAM在成年大鼠脊髓和背根神经节(DRG)感觉神经元中表达。通过RT-PCR检测到在整个发育过程中(E14-成年)脊髓和背根神经节中PAMmRNA处于相似水平。如通过Western印迹和RT-PCR所示，在酵母聚糖处理大鼠24-48小时后PAM表达上调。
在脊髓和DRG中表达的主要腺苷酸环化酶异型体分别是AC5和6型和AC4和6型。在酵母聚糖处理脊髓后，没有观察到AC异型体表达的大的变化。因此，在来源于脊髓和DRG的膜制备物中，Gαs刺激的AC活性可以被PAM抑制。因此发现用针对PAM的反义寡核苷酸(而不是正义寡核苷酸)处理成年大鼠后增加了福尔马林诱导的爪收缩。相应地，与对照大鼠相比用针对PAM的反义寡核苷酸处理的大鼠脊髓cAMP的积累提高了。
将纯化的PAM加入脊髓裂解物中导致来自对照动物和酵母聚糖处理动物的脊髓裂解物中由Gαs刺激的AC活性受到抑制(图5b)。在30nMPAM时，在来自对照动物的脊髓裂解液中的Gαs刺激的AC活性降低50％并且在来自用酵母聚糖处理96小时的大鼠的裂解物中降低70％。
为了确定PAM是否在脊髓中表达，首先进行原位杂交。结果在成年大鼠的脊髓灰质而不是白质中检测到PAM mRNA的清晰信号(图1)。为了更精确地定义脊髓和DRG中表达PAM的细胞种群，使用对应于人PAM氨基酸残基135-153位和4601-4614位的肽产生了针对PAM的抗体。免疫组织化学分析表明，PAM与抗神经元特异性核蛋白(NeuN)共定位但不与抗胶质原纤维酸性蛋白(GFAP)发生免疫反应(图2a)。更加具体而言，PAM主要在背角神经元表达(图2a)，而在非神经元的细胞群体PAM表达很少。在DRG神经元中检测到特别高的PAM表达(图2b)。在这里，PAM免疫反应定位于大直径和小直径神经元的轴突和细胞体(图2b)。有趣地是，通过抗组蛋白抗体的共染色证实在细胞核内未检测到PAM(图2b)。如在脊髓中看到的一样，在表达GFAP的细胞内未检测到PAM表达。
由于在大鼠和小鼠发育过程中，大脑中PAM的表达是差异调节的(Yang等，2002)，本发明者研究了PAM mRNA的表达是否在大鼠脊髓和DRG发育过程中也发生变化。为了实现该目的，使用定量RT-PCR检测PAM mRNA的表达。发现在胚胎晚期(E16)至出生后不久(P0.5)脊髓和DRG内PAM mRNA高度表达(图3)。
有趣地是，在出生后不久表达量降低至胚胎期表达量的30-40％，然后在整个成年期保持恒定(图3)。
下一步检测了脊髓中PAM的表达是否受伤害性刺激的调节。因此，在成年大鼠后爪注射酵母聚糖和福尔马林后，检测了脊髓PAM表达。在酵母聚糖处理24和48小时后，PAM mRNA上调了大约两倍(图4a)。相应地，在注射酵母聚糖24小时后，PAM的蛋白质水平表达上调，并且至96小时维持着提高的水平(图4b)。在注射酵母聚糖96小时后，PAM mRNA表达下降了。PAM mRNA表达的这种减少没有反映在蛋白质水平(比较图4a和b)。值得注意地是，在注射福尔马林1小时后，大鼠脊髓内PAMmRNA也上调了(图4c)。
由于已知PAM是腺苷酸环化酶1、5和6型的有力抑制剂(Scholich等，2001)，因此研究了PAM能否抑制脊髓和DRG裂解物中AC活性。通过半定量RT-PCR检测了脊髓内两个主要的AC异型体。这些AC异型体是5和6型(图5a)。值得注意地是，该两个异型体能够被纳摩尔浓度的PAM抑制(Scholich等，2001)。在注射酵母聚糖24-96小时后，脊髓内AC异型体表达模式无显著变化。有趣地是，在注射福尔马林1小时后，检测到AC异型体表达的改变(图5a)。AC 5型的mRNA下调并且AC3和9型的mRNA上调。
如通过上述实验所示，向脊髓裂解物中加入纯化的PAM导致脊髓制备物中Gαs刺激的AC活性的抑制。在对照组动物的脊髓裂解物中加入30nM PAM，使Gαs刺激的AC活性降低49％(图5b)。与未处理的大鼠相比，在注射酵母聚糖96小时后大鼠脊髓裂解物中Gαs刺激的AC活性表现出对PAM抑制更敏感(在30nM PAM时抑制70％；图5b)。。与此不同，在福尔马林处理1小时后的动物脊髓裂解物中，通过PAM的AC活性抑制的程度较小(30nM PAM时抑制25％；图5b)。
在DRG中主要表达的AC异型体是4和6型(图5a)。与脊髓裂解物相比，在30nM PAM存在时DRG裂解物内Gαs刺激的AC活性降低32％(图5b)。
为了检测PAM在脊柱伤害性传递中的可能作用，在进行福尔马林测试之前，通过腰部鞘内导管给动物注射PAM正义和反义寡核苷酸。如免疫组织化学结果所示，脊髓神经元中PAM表达降低(图6a)。与PAM正义寡核苷酸处理相比，注射PAM反义寡核苷酸导致在福尔马林注射后伤害性反应的显著增加(p＝0.007；图6b和c)。在PAM反义寡核苷酸处理的大鼠中痛觉增敏伴随着舔和咬行为的增加。由于PAM是AC活性的抑制剂(Scholich等，2001)，在正义和反义ODN处理大鼠的脊髓裂解物中测定了基础AC活性、Gαs和毛喉素刺激的AC活性。实验表明，反义ODN处理大鼠的基础AC活性显著升高(20.7％)(表1)。相反，在Gαs和毛喉素刺激的AC活性没有明显改变，这表明通过ODN处理不能改变AC的总量(表1)。
以上实验表明，PAM定位于脊髓和DRG神经元的细胞体和轴突(图2a，b)。在细胞核内仅有很少的免疫反应，这表明PAM在神经元和癌细胞系内的作用是不同的。
在延长伤害性刺激后的中枢致敏是基于脊髓中神经元和突触的变化(Woolf和Costigan，1999；Wool和Salter，2000；Ji和Woolf，2001)。本发明者发现PAM在脊髓和DRG的感觉神经元中表达，该发现导致形成了新的假说，即在脊髓伤害过程中突触的变化可能牵涉到PAM。伤害性刺激后PAM上调的上述发现(图4a)支持该假说，即PAM在脊髓伤害性过程的突触的变化中可能起作用。
此外，本发明者令人惊奇的发现(即PAM在脊髓和DRG的感觉神经元表达)引出一个问题，即PAM是否也能够抑制脊髓和DRG中的AC活性。
以上实验第一次表明，PAM是脊髓制备物中Gαs刺激AC活性的有效抑制剂(图5b)。加入30nM PAM后AC活性降低50％。为了实现使用抑制性G蛋白的α亚基Gαi的可比性的抑制，必须使用200-800nM的Gαi(Wittpoth等，1999)。在用酵母聚糖处理96小时后动物脊髓制备物中，PAM的抑制作用甚至更强(图5b)，这可以解释为在酵母聚糖注射后脊髓中内源性PAM的数量增加了。与对照或酵母聚糖处理动物(分别为50％和75％抑制)相比，在福尔马林处理动物的脊髓制备物中Gαs刺激的AC活性的抑制(25％抑制)更不明显。
值得注意，在用福尔马林处理1小时的动物中观察到AC异型体表达的变化(图5a)。AC的3型和9型上调而5型下调。迄今为止，尚不知PAM是否是AC3型和9型的抑制剂。因此，这些异型体可能不受PAM抑制，或者所检测的PAM浓度太低以致于不能产生抑制效应。由于PAM是一个分子量为510kDa的巨大蛋白质，测试大于30nM的PAM浓度在技术上是不可能的。虽然如此，根据表5b所示的剂量反应曲线，可见较高的PAM浓度能够导致所测试脊髓制备物中Gαs刺激的AC活性的较强抑制。
有趣地是，与对脊髓制备物中AC酶活性的抑制相比，PAM对DRG的AC酶活性的抑制较差。在脊髓和DRG制备物中PAM的不同抑制效率最大可能是由于AC异型体表达的不同。在脊髓中表达的主要AC异型体是5和6型，该两种AC能够被PAM强烈抑制(图5a；(Scholich等，2001))。在DRG中主要的AC异型体是4和6型(图5a)。因为尚不知PAM是否抑制AC 4型或该异型体是否不受PAM抑制，或者如上述情况所检测的PAM浓度是否太低以致于不能产生抑制效应。但是，根据图5b所示的剂量反应曲线，很可能更高浓度的PAM会导致DRG制备物中Gαs刺激的AC活性的更强烈的抑制。
然而，最令人惊奇地是PAM活性影响测试动物的伤害性行为的发现。这通过本发明者的实验第一次证明，该实验表明与PAM正义寡核苷酸处理相比，当通过给动物注射PAM反义寡核苷酸使得脊髓内源性PAM表达降低时(图6a)，基础AC活性显著增加并且更重要地是对福尔马林注射后的伤害性反应显著增加。
19.PAM镇痛作用的测定以上列出的关于PAM的镇痛作用的证据可以通过例如下面的假设实验得到支持例如在急性疼痛的福尔马林模型中，可通过鞘内使用例如对应于氨基酸残基1028-1065的肽直接测定PAM的镇痛作用。如通过酵母双杂交系统和AC活性测定法所确定，该肽代表介导PAM-腺苷酸环化酶相互作用的最小区域。肽可以以与bioporter脂质体试剂(可购自Peqlab，Germany)的复合物应用。该方法可以允许肽进入组织并且模拟生理性PAM对AC的作用。
20.证明S1P影响PAM信号的结果为了研究PAM在HeLa细胞内的表达和定位，采用了两个针对PAM的抗体，它们针对对应于人PAM氨基酸残基135-153和4601-4614的肽。对大鼠脑免疫组织染色的表较表明，两个抗体识别相同的脑部区域，同样该区域也表现为PAM mRNA的表达(Yang等，2002)。在血清饥饿的HeLa细胞中，两个抗体都显示PAM与钙联接蛋白(一种内质网标记)共定位(图18a)。向细胞中加入血清后，发现部分PAM向细胞膜易位(图18b)。在血清处理20-30分钟后PAM在细胞膜上出现并且在血清孵育1小时后PAM开始从细胞膜上消失。使用所用抗体观察到的HeLa内PAM的细胞内分布与Guo等描述的细胞内分布不同。因为Guo等使用PAM的一部分生产的抗体，而该部分包括核内蛋白的共同基序，因此该抗体与核内蛋白的交叉反应是可能的。
因为PAM是AC酶活性的强烈抑制剂，下一步研究了PAM从内质网到细胞膜的易位是否导致AC活性的抑制。血清处理的HeLa细胞降低细胞内cAMP的积累(图19a)。此外，与未处理细胞相比，血清处理的细胞内Gαs和毛喉素刺激的AC活性分别降低至56.7％和64.7％(图19b)。由于没有检测到AC异型体mRNA表达的变化，所以所观察到的AC活性的降低并不是因为AC异型体表达的变化或者AC表达的增加(图19c)。为了确定所刺激的AC活性的降低是否由PAM介导，如先前在Scholich等，2001中所述采用针对PAM的反义寡核苷酸降低内源性PAM的数量。如图19d所示，与正义或突变的反义ODN处理的细胞相比，在用反义ODN处理的HeLa细胞中PAM数量降低了(如通过Western印迹分析所确定)。使用抗Hsp70抗体对同一印迹膜再次杂交表明蛋白质的上样量相同(图19d)。然而，使用反义ODN处理的HeLa细胞明显降低Gαs和毛喉素刺激的AC活性的血清诱导抑制(图19e)。更重要地是，正义或突变体寡脱氧核苷酸转染HeLa细胞不影响Gαs和毛喉素刺激的AC活性的血清诱导抑制(图19e)。这些数据表明用血清刺激HeLa细胞后内源性PAM对AC活性起到抑制性作用。
为了鉴定能够诱导PAM易位至细胞膜的血清中的因子，使用反相柱、阴离子交换柱和凝胶过滤柱纯化因子。在每一纯化步骤之后，测试级分诱导PAM易位和AC抑制的能力。根据纯化特性，血清因子可以鉴定为是轻微疏水(使用0.3M NaCl从Phenyl-Sepharose柱洗脱)、带有强的负电荷(在0.7M NaCl时从MonoQ和Q-Sepharose柱洗脱)并且根据在superdex30凝胶过滤柱中的保留时间估计分子量小于500。跟据物理特性，测试了几种候选物质，其中只有鞘氨醇-1-磷酸盐能诱导PAM易位。
S1P能够与5个G蛋白偶联受体的一个家族结合。因此，通过半定量RT-PCR研究了HeLa细胞是否表达S1P受体。在HeLa细胞中，检测到了5个S1P受体异型体中的4个(S1P1-4)的mRNA(图20a)。接着，检测纯化的S1P是否与血清一样表现出PAM活化/易位的特性。首先，用浓度不断增加的纯化S1P处理HeLa细胞。当用0.1-5μM S1P处理细胞时，70-90％的细胞中出现PAM向细胞膜易位。在500nM S1P孵育10分钟后，PAM在细胞膜上出现并且在孵育1小时后PAM开始消失(图3b，c)。最重要地是，用0.5μM S1P处理HeLa细胞降低了细胞内cAMP含量(图21a)和Gαs刺激的AC活性(图21b)。在S1P孵育之后和部分恢复之前的3分钟时间内，Gαs刺激的AC活性下降。在S1P处理开始后5-10分钟内，Gαs刺激的AC活性再次下降(图4b)。在S1P孵育后的第60分钟时，针对PAM的反义ODN消除了Gαs刺激的AC活性的抑制作用(图21c)。总起来看，这些数据首次证实在HeLa细胞中S1P对AC活性的抑制作用通过两条不同的机制实现一种是快速的PAM不依赖性AC抑制作用(在S1P处理3-10分钟内)，另一种是延迟的PAM依赖性AC抑制作用(在S1P处理10-60分钟内)。
已经证明S1P能够通过结合其各个受体有效活化4个不同的G蛋白，即Gi、Gq、G12和G13(Hla等，Science，2001；Kluk等，BBA，2002；Siehler和Manning，BBA，2002；Spiegel和Milstein，JBC，2002)。其中只有Gi对百日咳毒素敏感。百日咳毒素处理消除了S1P对Gαs刺激的AC活性的抑制作用(图22a)以及PAM向细胞膜的易位(图22b)。因此，看起来可能是抑制性G蛋白Gi负责S1P所导致的快速的PAM不依赖性抑制作用。
接着阐明了引起PAM易位和活化的信号转导途径中的更多元件。由于已经描述过S1P1-4受体与Gi、Gq和G12/13偶联(Hla等，2001；Kluk等，2002；Siehler等，2002；Spiegel等，2002)并且PAM易位以及AC抑制是百日咳毒素依赖的(图22a)，以上数据表明HeLa细胞中PAM的活化依赖于Gi的活化。以前已经描述过S1P能够通过Gi的活化而激活磷脂酶C(PLC)以及ERK1/2信号(Kluk等，2002；Siehler等，2002；Spiegel等，2002)。因此，检测了PLC的活化是否参与PAM易位，发现在PLC抑制剂U73122存在下PAM易位和后期的AC抑制作用消失(图22a)。PLC将磷脂酰肌醇4，5-二磷酸转变成1，4，5-三磷酸肌醇(IP3)(一种动员钙的第二信使)和1，2-二酰基甘油(DAG)(蛋白激酶C(PKC)的活化剂)(Rebecchi等，2000；Wilde等，2001)。钙成像显示S1P诱导HeLa细胞中PLC依赖的钙增加。然而，该种钙的降低对PAM易位不是必需的，因为使用BAPTA-AM预处理不干扰PAM易位(图22a)。但是PKC抑制剂GF109203X和RO31-8220分别消除了PAM易位和AC抑制作用(图22a)。这些数据表明，S1P通过抑制性G蛋白Gi激活PLC。随后，PLC的作用导致了钙不依赖性的PKC激活，这对于介导PAM易位和较后的S1P诱导的AC抑制作用是必需的。
由于也已经证明S1P能够活化ERK1/2信号转导途径(Kluk等，2002；Siehler等，2002；Spiegel等，2002)，所以测试了在HeLa细胞中ERK1/2是否能被S1P活化。在用S1P孵育HeLa细胞之后，使用抗活化ERK和抗磷酸化Tyr183ERK抗体检测ERK1/2的磷酸化(图22b)。令人惊奇地是，ERK1/2磷酸化依赖于PLC活化(图22b)、Gi和PKC活性(数据未显示)但是不依赖于细胞内钙的增加。然而，ERK1/2的活化对于PAM易位或AC活性抑制作用不是必需的(图22a)。总之，这些发现首次证明S1P通过包括Gi、PLC和PKC在内的信号级联放大诱导PAM易位和其后的AC酶活性的抑制。有趣地是，S1P还诱导ERK1/2活化和细胞内钙浓度的增加，该两者对PAM易位或Gαs刺激的AC活性的抑制都不是必需的。
被S1P调节的信号转导途径是广泛研究的焦点(Kluk等，2002；Siehler等，2002；Spiegel等，2002)。众所周知，S1P受体通过Gi依赖机制能够抑制AC活性(Kluk等，2002；Siehler等，2002；Spiegel等，2002)。然而，在这里首次证明在S1P刺激后AC活性的长时间抑制并不是因为通过抑制性G蛋白的直接抑制作用，因为较后的AC抑制作用依赖于PLC和PKC的活化。此外，本发明者的数据表明，在S1P处理后HeLa细胞内AC活性的延长的抑制作用依赖于PAM向细胞膜的易位/激活。该易位受Gi活化和PLC/PKC信号的调节。由于PAM是AC酶活性的有效抑制剂，因此S1P诱导的PAM依赖性AC抑制作用可能是PAM和AC直接相互作用的结果，尽管这还未得到证实。
本发明者的以上实验导致首次发现PAM定位于HeLa细胞内质网并且在血清处理后PAM易位至细胞膜。PAM易位伴随着较之未处理的HeLa细胞相比细胞内Gαs和毛喉素刺激的AC活性的下降。由于用PAM的反义寡核苷酸预处理细胞阻止了AC的抑制作用，所以AC的抑制作用是由PAM介导的。之后，我们鉴定出鞘氨醇-1-磷酸(S1P)作为血清因子负责PAM的易位。用0.1-5μM S1P处理HeLa细胞，在10-30分钟内诱导80％的细胞出现PAM易位至细胞膜。S1P通过两种独立的机制降低AC活性。起始的AC抑制作用不是通过PAM介导的但是是百日咳毒素敏感的。在长时间S1P处理后，AC抑制作用依赖于PAM易位。S1P对PAM易位和PAM介导的AC抑制的作用是百日咳毒素敏感的并且需要PLC和PKC的活化。总起来看，这些数据第一次鉴定出PAM活性的调节物。而且，结果表明，S1P对AC活性的长期抑制作用是通过AC抑制性蛋白质PAM从内质网到细胞膜的易位介导的。
21.S1P镇痛作用的检测为了检测S1P的镇痛作用，通过鞘内使用将S1P递送至脊髓。
21a)腰鞘内导管的植入用氯胺酮(60mg/kg，腹腔注射)和咪达唑仑(0.5-1mg/kg，腹腔注射)麻醉大鼠。切开从第13胸椎至第3腰椎的脊柱外皮肤。去除第2-3腰椎周围的肌肉组织。去除第3腰椎的棘突，第2腰椎行椎板切除术。然后，将聚乙烯导管(ID 0.28mm，OD 0.61mm)插入硬膜下隙以致于导管顶部可到达第9-10胸椎。将导管用氰基丙烯酸酯胶固定，置于颈部区域表面并缝合皮肤。
21b)PAM寡核苷酸的注入术后3天，将鼠置入“自由活动系统”(CMA，Stockholm，Sweden)，通过导管注入20μl 10μM的S1P。
21c)福尔马林测试在停止注入15分钟内进行福尔马林测试。将50μl 5％甲醛溶液经皮下注射(s.c.)到一只后爪的背侧面。注射福尔马林后立即以1分钟的间隔计算收缩次数，直至60分钟。每间隔5分钟的收缩次数总结为每分钟的平均收缩次数。为了比较组间的伤害性行为，将1小时观察时间内的收缩次数的总和进行Students t检验。福尔马林测试结束后，切除腰脊髓和背根神经节(DRG)，在液氮中迅速冷冻并储存于-80℃直至进一步分析。
21d)结果在福尔马林注射前15分钟，通过鞘内给予成年大鼠20μl 10μM的S1P或20μl PBS/DMSO。然后，在60分钟阶段内以5分钟的间隔计数收缩次数。如通过与PBS/DMSO处理的动物相比，检测到在2A期(福尔马林注射后20-35分钟内)伤害性反应的次数显著减少(见图24)。这些实验清楚地证明外源性S1P具有镇痛作用。
22.S1P受体激动剂的镇痛/抗伤害作用的测定例如，S1P受体激动剂如FTY720的镇痛/抗伤害作用可通过以下假定实验得以证实如在福尔马林急性疼痛模型中，通过鞘内或静脉施用如FTY720可直接测定FTY720的镇痛/抗伤害作用，并且可通过如收缩试验连续测试其镇痛/抗伤害作用。这种方法允许分子进入组织，并且模拟生理性S1P对AC的作用。
参考文献Bailey C.H.，Bartsch D.，Kandel E.R.Toward a molecular definition oflong-term memory storage.Proc.Natl.Acad.Sci.U.S.A.1996年11月26日；93(24)13445-52；Brandon E.P.，Idzerda R.L.，McKnight G.S.PKA isoforms，neuralpathways，and behaviourmaking the connection.Curr.Opin.Neurobiol.1997年6月；7(3)397-403；Bek M.J.，Zheng S.，Xu J.，Yamaguchi I.，Asico L.D.，Sun X.G.和Jose P.A.(2001)Differential expression of adenylyl cyclases in the ratnephron.Kidney Int 60，890-899；Brinkmann，V.，Davis，M.D.，Heise，C.E.，Albert，R.，Cottens，S.，Hof，R.，Bruns，C.，Prieschl，E.，Baumruker，T.，Hiestand，P.，Foster，C.A.，Zollinger，M.和Lynch，K.R.，(2002)，TheImmune Modulator FTY720 Targets Sphingosine-1-PhosphateReceptors；the Journal of Biological Chemistry，第277卷，第24期，6月14日，21453-21457页；Caligan，T.B.，Peters，K.，Ou，J.，Wang，E.，Saba，J.和Merrill，A.H.，Jr.(2000)Anal Biochem 281，36-44；Chang Q.和Balice-Gordon R.J.(2000)Highwire，rpm-1，and futschbalancing synaptic growth and stability.Neuron 26，287-290；Chen，Z.，Nield，H.S.，Sun，H.，Barbier，A.，和Patel，T.B.(1995)JBiol Chem 270，27525-27530；Chomczyns ki，P.，Sacchi，N.Single-step method of RNA isolation by acidguanidinium thiocyanate-phenol-chloroform extraction.Anal.Biochem.162，(1987)156-159；DiAntonio，A.，Haghighi，A.P.，Portman，S.L.，Lee，J.D.，Amaranto，A.M.和Goodman，C.S.(2001)Nature 412，449-452Graziano，M.P.FreissmuthM.Gilman A.G.Purification of recombinantGs alpha.Meth.Enz.(1991)195192-215；Graziano M.P.，Freissmuth M.和Gilman A.G.(1991)Purification ofrecombinant Gs alpha.Methods Enzymol 195，192-202；Guo Q.，Xie J.，Dang C.V.，Liu E.T.，Bishop J.M.Identification of alarge Myc-binding protein that contains RCC1-like repeats.Proc.Natl.Acad.Sci.U.S.A.1998年8月4日；95(16)9172-7；Grossberger，R.，Gieffers，C.，Zachariae，W.，Podtelejnikov，A.V.，Schleiffer，A.，Nasmyth，K.，Mann，M.和Peters，J.M.(1999)J Biol Chem274，14500-14507；Hla，T.，Lee，M.J.，Ancellin，N.，Paik，J.H.，和Kluk，M.J.(2001)Science294，1875-1878；Jin Y.(2002)Synaptogenesisinsights from worm and fly.Curr OpinNeurobiol 12，71-79；Julius和Basbaum“Molecular mechanisms of nociception”，Nature，第413卷，13，2001年9月，203-209；Kassis，S.和Fishman，P.H.(1982)J Biol Chem 257(9)，5312-5318；Kind，P.C.和Neumann，P.E.(2001)Trends Neurosci 24，553-555；Kluk，M.J.和Hla，T.(2002)Biochim Biophys Acta 1582，72-80；Mandala，S.，Hajdu，R.，Bergstrom，J.，Quackenbush，E.，Xie，J.，Milligan，J.，Thornton，R.，Shei，G.，Card，D.，Keohand，C.，Rosenbach，M.，Hale，J.，Lynch，C.L.，Rupprecht，K.，Parsons，W.和Rosen，H.，(2002)，Alteration of Lymphocyte Trafficking by Spingosine-1-PhospateReceptor Agonists，Science，第296卷，2002年4月；Meller S.T.，Gebhart G.F.(1997)，intraplantar zymosan as a reliable，quantifiable model of thermal and mechanical hyperalgesia in the rat；Eur.J.Pain 1，43-52；Nair，B.G.，Parikh，B.，Milligan，G.和Patel，T.B.(1990)J Biol Chem265(34)，21317-21322；Nestler，E.J.(2001)Nat Rev Neurosci 2，119-128；Patel T.B.，Wittpoth C.，Barbier A.J.，Yigzaw Y.和Scholich K.(2002)Functional analyses of type V adenylyl cyclase.Methods Enzymol 345，160-187.
Snyder S.H.(1985)Adenosine as a neuromodulator.Annu Rev Neurosci8，103-124；Payne，S.G.，Milstien，S.和Spiegel，S.(2002)，Sphingosine-1-phosphatedual messenger functions；FEBS Letters 531(2002)，54-57；Postma，F.R.，Jalink，K.，Hengeveld，T.和Moolenaar，W.H.(1996)EmboJ 15，2388-2392.
Rebecchi，M.J.和Pentyala，S.N.(2000)Physiol Rev 80，1291-1335.
Ruppert C.，Goldowitz D.和Wille W.(1986)，Proto-oncogene-c-myc isexpressed in cerebellar neurons at different developmental stages，EmboJ5，1897-1901；Sato，K.，Tomura，H.，Igarashi，Y.，Ui，M.和Okajima，F.(1997)BiochemBiophys Res Commun 240，329-334.
Schaefer A.M.，Hadwiger G.D.和Nonet M.L.(2000)rpm-1，aconserved neuronal gene that regulates targeting and synaptogenesis in C.elegans.Neuron 26，345-356.
Schaible H.G.，Vanegas H.How do we manage chronic pain？BaillieresBest.Pract.Res.Clin.Rheumatol.2000年12月；14(4)797-811；Scherr M.，Morgan M.A.，Eder M.，Gene Silencing Mediated by SmallInterfering RNAs in Mammalian Cells，Curr Med Chem.2003年2月；10(3)245-256；Scholich，K.，Mullenix，J.B.，Wittpoth，C.，Poppleton，H.M.，Pierre，S.C.，Lindorfer，M.A.，Garrison，J.C.和Patel，T.B.(1999)Science 283，1328-1331.
Scholich K.，Pierre S.，Patel T.B.Protein associated with myc(PAM)is apotent inhibitor of adenylyl cyclase.J.Biol.Chem.2001年12月14日；276(50)47583-9；Scholz和Woolf“Can we conquer pain”，Nature neurosciencesupplement，第5卷，2002年11月，1062-1067；Siehler，S.和Manning，D.R.(2002)Biochim Biophys Acta 1582，94-99.Snyder S.H.(1985)，Adenosine as a neuromodulator.Annual Reviews ofNeuroscience 8，103-104；Spiegel，S.和Milstien，S.(2000)，Functions of a new family ofsphingosine-1-phosphate receptors，Biochimica et Biophysica Acta 1484，107-116；Spiegel，S.和Milstien，S.(2002)J.Biol.Chem 277；25851-25854；Trajkovic V，Samardzic T，Stosic-Grujicic S，Ramic Z，MostaricaStojkovic M.Muramyl dipeptide potentiates cytokine-induced activation ofinducible nitric oxide synthase in rat astrocytes.Brain Res.2000年11月10日；883(1)157-63；Wan H.I.，DiAntonio A.，Fetter R.D.，Bergstrom K.，Strauss R.和Goodman C.S.(2000)Highwire regulates synaptic growth in Drosophila.Neuron 26，313-329；West，A.E.，Chen，W.G.，Dalva，M.B.，Dolmetsch，R.E.，Kornhauser，J.M.，Shaywitz，A.J.，Takasu，M.A.，Tao，X.，a和Greenberg，M.E.(2001)Proc Natl Acad Sci U S A 98，11024-11031；Wilde，J.I.和Watson，S.P.(2001)Cell Signal 13，691-701.Wittpoth C.，Scholich K.，Yigzaw Y.，Stringfield T.M.和Patel T.B.(1999)，Regions on adenylyl cyclase that are necessary for inhibition of activity bybeta gamma G(iα)subunits of heterotrimeric G proteins.Proc.Natl.Acad.Sci.USA 96，7723-7730；Wood，J.D.“Pathobiology of Visceral PainMolecular Mechanisms andTherapeutic Implications II.genetic approaches to pain therapy”，American Journal pf Physiological Gastrointestinal Liver Physiology，2000，第278卷，G507-G512；Woolf和Mannion“Neuropathic painaetiology，symptoms mechanisms，and management”，The LANCET，第353卷，1999年6月5日，1959-1964；Woolf J.和Salter M.W.“Neuronal PlasticityIncreasing the Gain inPain”，Science，第288卷，2000年6月9日，1765-1768；Xia Z.，Storm D.R.Calmodulin-regulated adenylyl cyclases andneuromodulation.Curr.Opin.Neurobiol.1997年6月；7(3)391-6；Xu，D.，Isaacs，C.，Hall，I.P.和Emala，C.W.(2001)Am J Physiol LungCell Mol Physiol 281，L832-843.
Yang H.，Scholich K.，Poser S.，Storm D.，Patel T.B.，Goldowitz D.Developmental expression of protein associated with myc(PAM)in therodent brain.Brain Res Dev Brain Res 136，2002，35-42；Zhen M.，Huang X.，Bamber B.和Jin Y.(2000)Regulation of presynapticterminal organization by C.elegans RPM-1，a putative guanine nucleotideexchanger with a RING-H2 finger domain.Neuron 26，331-343.
实验室方法的标准参考文献如果没有另外指出，按照以下标准参考文献开展或可以开展实验室方法Sambrook等，(1989)Molecular CloningA Laboratory Manual.第二版，Cold Spring Harbor Laboratory Press，Cold Spring Harbor，NY.545页或者Current Protocols in Molecular Biology；Current Protocols in Molecular Biology；定期更新，例如2000卷；JohnWiley & Sons，Inc；著者Fred M.Ausubel，Roger Brent，Robert Eg.Kingston，David D.Moore，J.G.Seidman，John A.Smith，Kevin Struhl.Current Protocols in Human Genetics；定期更新，例如2003卷；John Wiley&Sons，Inc；著者Nicholas C.Dracopoli，Honathan L.Haines，Bruce R.Korf，Cynthia C.Morton，Christine E.Seidman，J.G.Seigman，DouglasR.Smith.
Current Protocols in Protein Science；定期更新，例如2003卷；John Wiley&Sons，Inc；著者John E.Coligan，Ben M.Dunn，Hidde L.Ploegh，DavidW.Speicher，Paul T.Wingfield.
Molecular Biology of the Cell；第三版；Alberts，B.，Bray，D.，Lewis，J.，Raff，M.，Roberts，K.，Watson，J.D.；Garland Publishing，Inc.NewYork & London，1994；
Gene Targetinga practical approach(1995)，著者A.L.Joyner，IRLPressGenetic Manipulation of Receptor Expression and Function；D.Accili，Wiley-Liss.，USA，2000；ISBN0-471-35057-5.
Antisense Therapeutics，S.Agrawal，Humana Press，USA，1996，ISBN0-89603-305-8.
Remington′s Pharmaceutical Sciences，第17版，1985.
所使用的缩写AC，腺苷酸环化酶；Gαs，腺苷酸环化酶刺激性G蛋白的α亚基；Gαs*，Gαs的组成性活化(Q213L)突变体；Gαi，抑制性G蛋白Gi的α亚基；Gβγ，异源三聚体G蛋白的βγ亚基；ODN，寡脱氧核苷酸；PAM，Myc相关蛋白质；RCC1，染色体浓缩调节蛋白；S1P，鞘氨醇-1-磷酸盐；TED，50mM Tris-HCl，pH8.0，1mM EDTA，1mM DTT；RT，室温；图图1PAM在脊髓神经元高度表达。在原位杂交中，使用上述的针对大鼠PAM的正义链探针或反义链探针与脊髓水平切面杂交。
图2PAM在大鼠DRG神经元和脊髓神经元细胞中表达。
栏A大鼠脊髓切片的免疫组织化学分析。将切片用抗PAM抗体(绿色)及抗NeuN或抗GFAP抗体(红色)染色以分别显示神经元或神经胶质细胞。两个信号的叠加在右侧图中显示。物体放大20倍。
栏B大鼠DRG切片的免疫组织化学分析。将切片用抗PAM抗体(绿色)及抗Neu68、抗Histon或抗GFAP抗体(红色)染色，以分别显示神经元、细胞核或神经胶质细胞。两个信号的叠加在右侧图中显示。除了Histon染色放大63倍外，其它物体放大40倍。
图3在不同发育阶段的DRG和脊髓中，PAM差异表达。使用定量RT-PCR(TaqmanTM)在第16天胚胎大鼠(E16)、出生后0.5天(P0.5)、出生后3天(P3)、出生后5天(P5)、出生后9天(P9)及成年大鼠的脊髓和DRG的RNA(40ng)中检测PAM。显示为至少3次检测结果的平均值±SEM。图4酵母聚糖和福尔马林处理后，大鼠脊髓中PAM上调。
栏A对来自对照大鼠和酵母聚糖处理24h、48h和96h大鼠的脊髓RNA(40ng)进行RT-PCR分析。本栏中下面的图中显示了7次实验的平均值±SEM。采用Student T检验*p＜0.001。
栏B对来自对照大鼠和酵母聚糖处理24h、48h和96h大鼠的脊髓裂解液(40μg)进行7％SDS-PAGE凝胶电泳，并使用抗PAM抗体和抗ERK1/2抗体开展Western印迹。
栏C对来自对照大鼠或福尔马林处理1小时的大鼠的脊髓RNA(40ng)进行定量RT-PCR分析。
图5PAM抑制脊髓裂解物中Gαs刺激的AC活性。
栏A使用RT-PCR检测脊髓和DRG RNA(40ng)中AC异型体的表达。
栏B如材料和方法部分所述，分析在80nM Gαs存在下脊髓或DRG裂解物中(10μg)的AC活性。显示为至少3次测定结果的平均值±SEM。
图6鞘内应用针对PAM的反义ODN增加了伤害性行为。
栏A如所述对成年大鼠鞘内给予正义和反义ODN。福尔马林处理后，取出脊髓，并使用抗PAM抗体(绿色)或抗NeuN抗体(红色)进行免疫组织学分析。
栏B如材料部分所述用正义或反义ODN处理动物后进行福尔马林测试。显示1小时内的总收缩次数。显示为至少4次测定结果的平均值±SE。
栏C如材料部分所述用正义或反义ODN处理动物后进行福尔马林测试。显示了1小时过程中的收缩次数。显示为至少4次测定结果的平均值±SE。
图7根据NCBI登录号AAC39928(蛋白序列；SEQ ID No.1)和AF075587(编码序列；SEQ ID No.2)的人PAM的蛋白质序列、基因组序列和编码核苷酸序列。人PAM定位于染色体13q22；其基因组序列可从NT_024524.11公开获得(起始24679861位；终止24962245位；SEQ IDNo.3)。图7C显示了24679861位至24962245位的连续序列。
图8大鼠PAM的EST-克隆编码序列图8AAW921303(对应于人cDNA的bp960-1394；SEQ ID No.4)图8BAW918711(对应于人cDNA的bp8188-8632；SEQ ID No.5)图8CBQ201485(对应于人cDNA的bp8966-9633；SEQ ID No.6)图8DBE112881(对应于人cDNA的bp10311-10830；SEQ ID No.7)图8EAW441131(对应于人cDNA的bp13569-14152；SEQ ID No.8)图8FBF409872(对应于人cDNA的bp13569-14807；SEQ ID No.9)。
图9人PAM结构域的结构概括(根据Guo等，1988/Grossberger等，JBC，1999和Scholich等，JBC，2001)图10用于大鼠PAM RT-PCR的PCR引物。
图11用于抑制大鼠PAM表达的反义寡脱氧核苷酸和对照寡核苷酸。
图12本发明文中使用的不同的人PAM多肽。多肽是来源于SEQ ID No.2的多肽的片段。
图13本发明文中使用的不同的人PAM多核苷酸。
图13A编码SEQ ID No.17的蛋白质片段并包含人PAM cDNA1317-4366位核苷酸的cDNA序列(SEQ ID No.24)；图13B编码SEQ ID No.18的蛋白质片段并包含人PAM cDNA1482-3332位核苷酸的cDNA序列(SEQ ID No.25)；图13C编码SEQ ID No.19的蛋白质片段并包含人PAM cDNA1641-3342位核苷酸的cDNA序列(SEQ ID No.26)；
图13D编码SEQ ID No.20的蛋白质片段并包含人PAM cDNA3142-4046位核苷酸的cDNA序列(SEQ ID No.27)；图13E编码SEQ ID No.21的蛋白质片段并包含人PAM cDNA3142-3446位核苷酸的cDNA序列(SEQ ID No.28)；图13F编码SEQ ID No.22的蛋白质片段并包含人PAM cDNA3228-3839位核苷酸的cDNA序列(SEQ ID No.29)；图13G编码SEQ ID No.23的蛋白质片段并包含人PAM cDNA3228-3341位核苷酸的cDNA序列(SEQ ID No.30)。
图14根据以上发现的PAM信号转导途径。
图15鞘氨醇-1-磷酸盐结构。
图16S1P受体的mRNA和氨基酸序列16a)S1P1氨基酸序列(SEQ ID No.31)；16b)S1P1mRNA序列(SEQ ID No.32)；编码序列起始于244位并终止于1392位；16c)S1P2氨基酸序列(SEQ ID No.33)；16d)S1P2mRNA序列(SEQ ID No.34)；编码序列起始于1位并终止于1062位；16e)S1P3氨基酸序列(SEQ ID No.35)；16f)S1P3mRNA序列(SEQ ID No.36)；编码序列起始于1位并终止于1137位；16g)S1P4氨基酸序列(SEQ ID No.37)；16h)S1P4mRNA序列(SEQ ID No.38)；编码序列起始于23位并终止于1177位；16i)S1P5氨基酸序列(SEQ ID No.39)；16j)S1P5mRNA序列(SEQ ID No.40)；编码序列起始于10位并终止于1206位；图17FTY720的结构图18血清刺激后，HeLa细胞中PAM从ER易位至细胞膜。
栏A如上所述将血清饥饿的HeLa细胞(24小时)固定并用抗PAM抗体(绿色)和抗钙联接蛋白抗体(红色)染色。
栏B用10％胎牛血清处理HeLa细胞不同时间并用抗PAM抗体染色以观察亚细胞定位。
图19通过PAM依赖的过程，血清处理的HeLa细胞内AC活性降低。
栏A如上所述，在血清缺乏和血清存在的情况下测定的HeLa细胞中cAMP的积累。显示为三次测定的平均值±SEM(平均值的标准差)。
栏B如材料和方法部分所述，在血清缺乏和存在的情况下测定的HeLa细胞裂解物中腺苷酸环化酶活性。Gsα*(80nM；灰色条)毛喉素(100μM；黑色条)刺激的AC特异活性分别为124±10pmol/mg/分钟和464±89pmol/mg/分钟。基础活性是11.75±2pmol/mg/分钟。显示为至少两次试验(每次实验3次重复测定)的平均值±SE。采用Student T检验*p＜0.001。
栏C使用RT-PCR分析血清饥饿和10％FBS孵育1小时后HeLa细胞中AC异型体的表达。
栏D如上所述，用3M反义、正义和包含三个点突变的反义(3M-as)ODN分别转染经血清饥饿的HeLa细胞。收获细胞、用7％SDS-PAGE(30μg蛋白质)分离并用抗PAM抗体和抗Hsp70抗体进行Western分析。
栏E如上所述，用反义、正义和3M-as ODN处理经血清饥饿的HeLa细胞。24小时后，用10％FBS孵育细胞30分钟。收获细胞并如上所述测定Gαs*(80nM；灰色条)和毛喉素(100μM；黑色条)存在时的AC活性。数据来自至少三次实验(每次实验重复测定3次)并表示为平均值±SEM。采用Student T检验*p＜0.01，**p＜0.05。
图20S1P诱导PAM易位至细胞膜并且抑制PAM依赖性的AC酶活性。
栏ART-PCR分析血清饥饿的HeLa细胞中S1P受体异型体的表达。
栏B用0.5μM S1P处理HeLa细胞30分钟并用抗PAM抗体染色以检测亚细胞定位。
栏CHeLa细胞中PAM易位的时间依赖性。用0.5μM S1P处理血清饥饿的HeLa细胞不同时间，并用抗PAM抗体染色以检测亚细胞定位。显示为出现PAM易位的细胞的百分数。
图21S1P处理的HeLa细胞通过PAM依赖性过程降低AC活性。
图A如材料和方法部分所述，在0.5μM S1P存在(黑色条)和不存在(灰色条)情况下，在不同的时间测定HeLa细胞中cAMP的积累。显示为三次测定的平均值±SEM。
栏B用0.5μM S1P处理不同时间的血清饥饿HeLa细胞。收集细胞并如材料和方法所述测定在80nM Gαs*存在下的AC活性。数据来自至少4次实验(每次实验重复测定3次)并表示为平均值±SE。
栏C如材料和方法部分所述，用正义、反义和3M-as ODN处理血清饥饿的HeLa细胞。24小时后，用0.5μM S1P孵育细胞达到指定时间。收集细胞并如上所述测定在Gαs*(80nM)存在下的AC活性。数据表示为三次测定的平均值±SEM。
图22PAM易位和PAM依赖性AC酶活性抑制作用是由PLC/PKC信号转导途径介导的。
栏A用0.5μM S1P处理血清饥饿的HeLa细胞3分钟(灰色条)或60分钟(黑色条)。在S1P孵育之前，将细胞用1μg/μl百日咳毒素(PTX)孵育24小时。在80nM Gαs*存在下测定AC活性。数据来自至少2次实验(每次实验重复测定3次)并表示为平均值±SE。
栏B用0.5μM S1P处理血清饥饿的HeLa细胞。在S1P孵育之前，将细胞用10μM U73122、RO31-8220、BAPTA-AM或U0126孵育20分钟。在80nM Gαs*存在下测定AC活性。数据来自至少2次实验(每次实验重复测定3次)并表示为平均值±SE。
栏C用0.5μM S1P处理10分钟的HeLa细胞的Western印迹分析。在S1P孵育之前，将细胞用10μM U73122、BAPTA-AM或U0126孵育20分钟。收获细胞，在Laemmli缓冲液中煮沸，并用抗活化ERK1/2抗体进行Western印迹分析。使用抗Hsp70抗体作为上样量对照。
栏D用0.5μM S1P处理HeLa细胞20分钟，并用抗PAM抗体染色以检测亚细胞定位。在S1P处理之前，用1μg/μl百日咳毒素(PTX)孵育细胞24小时或者用1μM GF109203X、10μM U0126、BAPTA-AM、U73122或Ro31-223孵育细胞20分钟。
图23所提出的导致PAM易位和PAM依赖性AC酶活性抑制作用的信号转导途径的模式图。
图24应用和不应用S1P 60分钟后，通过福尔马林测试的成年大鼠的疼痛行为。
在福尔马林注射前15分钟，通过鞘内应用给予成年大鼠20μl 10μMS1P或20μl PBS/DMSO。注射福尔马林后，在60分钟内以每5分钟的间隔计数收缩次数。显示为6次动物实验的平均值±SEM。
表1在反义ODN处理的大鼠脊髓裂解物中AC的基础活性增加。如上所述，在80nM Gαs或100μM毛喉素存在的情况下测定脊髓裂解物物(20μg)中的AC活性。每组至少3只大鼠，并且每次测定具有3个平行样并测定2次，结果表示为每组的脊髓裂解物的平均AC活性±SEM(ns＝无显著差异)。
序列表<110>塞诺菲-安万特德国有限公司(SANOFI-AVENTIS DEUTSCHLAND GMBH)<120>S1P的用途<130>DEAV 2003/0049<140>03012389.7<141>2003-05-30<160>40<170>PatentIn version 3.3<210>1<211>14807<212>DNA<213>人(Homo sapiens)<400>1tcttggagcg ttctcagttt ctcaacagat cttcacttgc taggcagcca gaagccggcg 60gcagtggcgg caccgcctcc tcctcacatt cccggggtgg cggggttaga tgagcggccc 120cagtcgcggc gccgggggcg ctgttcatgc cggttcccga cggctccgtg gctgctgcgg 180ggctggggct ggggctaccc gccgcggact ccccgggtca ctaccagctg ctgctgtcag 240gccgggccct ggccgaccgc taccggagga tttataccgc tgcgctcaat gacagggacc 300aggggggcgg cagcgctgga cacccagcct ccaggaataa gaaaatttta aataagaaga 360aattgaaaag aaaacagaag agcaaatcaa aagtgaagac aagaagcaag tctgaaaact 420tagagaatac agtaatcata ccagatatca aactacatag caatccttct gctttcaata 480tttactgtaa tgtacgccat tgcgttctgg aatggcagaa aaaggaaata tcattggcag 540ccgcatctaa gaactctgtg cagagtggag aatcagatag tgatgaagaa gaggaatcca 600aagagccccc tatcaagctt ccaaagatta ttgaggttgg cctttgtgaa gtttttgaat 660tgatcaaaga gacacgattt tctcatccat ccctgtgtct caggagtctc caagccctgc 720tcaacgtgct gcagggccag cagccagaag tgctccagtc tgagccacct gaggtcctag 780agtctctctt ccagcttctt ttggaaatca ccgttcgaag tactgggatg aatgacagca 840caggacagtc cttaacagca ctttcctgtg cttgcctctt tagtctggtg gcttcttggg 900gagaaacagg aaggacactt caggccatct ctgctatcct caccaacaat ggaagccatg 960cttgccaaac tattcaggtg ccaacaattc taaattcgct acagagaagt gtacaagcag 1020
ttttggtggg aaaaattcaa attcaggact ggtttagtaa tggcattaag aaagcagctt1080taatgcacaa gtggccatta aaagaaatat ctgttgatga agatgaccaa tgtctacttc1140agaatgatgg attttttctt tatctattat gcaaggatgg attatataaa ataggctctg1200gatacagtgg aacagttagg ggccatatat acaattctac atcccgtatt agaaacagaa1260aagaaaaaaa gtcttggtta gggtatgctc agggttattt attatataga gatgtgaata1320accacagcat gacagccata aggataagcc ctgaaacact ggagcaagat ggtactgtga1380tgttaccaga ttgccacact gaaggtcaaa atattttatt cactgatgga gaatatatta1440atcagatagc tgcttcaaga gatgatggct ttgttgtcag aatatttgcc acaagcactg1500aacctgttct acagcaagaa ttgcaactta aactggctag aaaatgctta catgcctgtc1560gtatctcatt attcgatctg gaaaaggact tgcatattat aagtacagga tttgatgagg1620agtcagcaat tcttggtgca ggacgagagt ttgcgctaat gaaaacagca aatggaaaga1680tatattacac tggcaaatac cagagtcttg gaatcaaaca aggtggtcct tcagcaggaa1740aatgggttga gctaccaatt acaaaatctc caaagatagt acacttctca gttggacacg1800atggctctca cgccctttta gttgcagaag atgggagcat attctttaca ggatctgcta1860gtaaaggaga agatggagaa tcaattaaga gcagacggca atccaaacct tataaaccta1920aaaagataat taagatggaa ggaaagattg tggtatatac agcctgcaat aatggaagta1980gttctgttat ttctaaagat ggagaactct acatgtttgg aaaagatgcc atttactctg2040atagttcaag tttggtaact gatttgaagg gccattttgt aactcaggta gctatgggca2100aagctcacac ttgtgtttta atgaagaatg gagaggtgtg gacatttggt gtaaataata2160aaggacagtg tggacgagat actggtgcca tgaaccaagg tgggaaaggg tttggagttg2220aaaatatggc aacagcaatg gatgaagacc tggaagaaga actagatgaa aaagatgaga2280agtctatgat gtgccctcca ggcatgcaca aatggaagct ggagcagtgc atggtttgca2340ctgtctgtgg agactgtaca ggttatggag ccagctgtgt cagtagtgga cggccagaca2400gagtccccgg agggatctgt ggttgtggtt ccggagaatc tggttgtgct gtgtgtggat2460gttgcaaggc ctgtgcaaga gagttagatg gtcaagaggc aagacaaaga ggaattcttg2520atgcagtgaa agaaatgata cctttagatc ttcttttagc tgtcccagtg cccggggtta2580acattgaaga acaccttcag ttacgacaag aagaaaaacg gcaacgtgta atcagaaggc2640acagattaga ggaaggaaga ggcccccttg tatttgctgg tcctattttt atgaaccatc2700
gagaacaggc tctagccaga ctcagatccc atccagcaca cgtaaagcat aaacgggaca2760agcacaaaga tggaagtgga gaaagaggcg aaaaggatgc aagcaaaatc acaacatacc2820ctccaggctc tgtgcgattt gactgtgagc tccgggcagt ccaagtcagc tgtggatttc2880accattcagt ggttttaatg gaaaatggag atgtctatac atttggttat gggcagcatg2940ggcagctagg acatggagat gtcaactcca ggggatgtcc cactcttgtt caagcattgc3000caggccctag cacacaagtc actgcaggca gcaaccatac ggcagtactt ttaatggatg3060gacaggtctt cacatttgga agtttttcta aaggacaact gggcagacca attttggatg3120tgccatattg gaatgcaaag ccagctccca tgcctaacat tggatcaaaa tatggaagaa3180aagctacttg gataggtgca agtggggacc aaactttttt acgaattgat gaagcactta3240ttaattctca tgtacttgct acatcagaaa tttttgccag taaacacata ataggcttgg3300tacctgcttc tatatcagaa cctcctccat ttaaatgcct tctgataaat aaagtggatg3360ggagttgtaa aacttttaat gactcagaac aagaggatct gcaaggattt ggtgtgtgtc3420ttgatcctgt atatgatgta atttggaggt ttcgaccaaa tactagagag ctgtggtgtt3480acaatgcggt ggttgctgat gccaggcttc cctctgcagc agacatgcag tccagatgta3540gtatcctaag tcctgaactt gccttaccaa caggatcaag ggccctcact acccgatctc3600atgcagcttt gcacatttta ggttgtcttg ataccttggc agctatgcag gacttaaaaa3660tgggtgttgc aagtacagag gaagagactc aagcagtaat gaaggtttat tctaaagaag3720attatagtgt ggtaaacagg tttgaaagtc atggaggagg ctggggttat tctgcccatt3780cagtagaagc tatacgtttc agtgccgaca ctgatatttt acttggtggt cttggtctgt3840ttggaggtag aggagaatat actgctaaaa ttaagctgtt tgaattgggt cctgatggag3900gagatcatga aactgatggt gaccttcttg cagagactga tgtattggct tatgactgtg3960ctgctagaga aaaatatgca atgatgtttg atgagcctgt tctcctgcaa gctgggtggt4020ggtatgtggc atgggcccga gtgtcaggac ccagcagtga ctgtggatct catggacagg4080catctattac cacagatgat ggggttgttt tccagttcaa gagttcaaag aaatcaaata4140atggtacaga tgttaatgcg ggtcagatac ctcagttatt atacagactt ccaaccagtg4200atggcagtgc ttcaaaaggc aaacagcaaa ccagtgaacc tgtacacatt ttaaagaggt4260cttttgcaag aactgtctca gtggaatgtt ttgagtcatt gttgagtatt cttcactgga4320gctggaccac cttagtctta ggagttgaag aacttagagg attaaaagga ttccagttca4380
cagctacact cctagattta gagagactgc gctttgtggg tacctgttgt ctgaggttat4440tgcgtgtcta tacctgtgaa atttacccag tgtcagctac aggaaaagca gttgtagaag4500aaactagcaa attagcagag tgtattggaa aaaccagaac tttgttaaga aaaattttat4560cagaaccact tgatcactgc atggtgaaat tggataatga tcctcaagga tatctcagtc4620aacccttgag tcttctagaa gctgtccttc aggaatgtca taatactttc actgcctgct4680ttcattcttt ctacccaact cctgccttac agtgggcttg cctttgtgat ctgctgaatt4740gtttggatca ggatatccaa gaagcaaact tcaagacatc aagtagccga ctccttgcag4800ctgttatgtc agctctgtgt cacacgtctg ttaagctgac ttccatcttc ccgattgcgt4860atgatggaga agtattacta cgatcaattg ttaaacaagt tagtacagag aacgactcaa4920cactagttca tcgttttccc cttttggtgg cacatatgga aaaactcagc cagagtgaag4980agaatatctc agggatgaca agcttccgtg aagttctgga gaaaatgctg gtcattgttg5040tgctaccagt caggaacagc ctgaggagag aaaatgaact cttctcctcc cacctcgtct5100ctaacacctg tggattactg gccagcattg tcagtgaact gacagcgtca gccctgggat5160ctgaggttga tggacttaat tctcttcact ctgtaaaagc tagtgctaac cgatttacaa5220aaacaagtca gggcagaagt tggaacactg ggaacgggtc ccctgatgca atctgttttt5280cagtagacaa acctggaata gttgtggttg gtttctctgt ctatggagga ggtggaattc5340atgaatatga attagaggtg ttggttgatg atagtgaaca tgcaggagat tcaactcatt5400cccacagatg gacatctctg gaattagtga aaggaacgta cacaacggat gactcaccca5460gtgatatagc tgagatcaga cttgacaaag tggttccttt aaaggaaaat gttaaatatg5520ctgtgcgctt gaggaactat ggaagccgta cagccaatgg agatggagga atgaccacag5580ttcagtgccc tgatggtgtg acattcacat tcagcacgtg cagcttgagc agtaacggca5640caaaccaaac cagaggacag atcccacaga tactctacta taggagtgaa tttgatggag5700atttacaatc ccaacttctg agtaaagcca atgaagaaga taaaaactgt agcagagcat5760tgtctgttgt aagcactgtc gttcgagcct ctaaggacct cctgcacaga gctcttgctg5820tggatgctga tgacattcca gaactgctga gttcttccag tctgttttcc atgctgctcc5880cccttattat agcctacata ggaccagtag ctgctgctat tcccaaggtg gctgtagaag5940tctttggcct tgtccaacaa ttgcttccgt cagttgccat tttgaatcag aagtatgcac6000cgcctgcctt caaccctaat cagtcgacag atagcaccac aggaaaccag cctgaacagg6060
gcctctctgc ttgtacaacc tccagtcact atgctgtcat agagagtgag cacccgtata6120aacctgcctg tgtgatgcat tacaaggtga cattcccaga atgtgtgagg tggatgacaa6180tcgaatttga ccctcagtgt ggtactgcac agtcagaaga tgtccttcgt ttgttgattc6240ctgtcagaac tgttcagaat tcaggatatg gaccaaaatt gacatctgtt catgaaaatc6300ttaattcatg gatagaatta aagaaatttt caggatcctc tgggtggcct actatggttt6360tggtgttgcc aggaaatgag gccctttttt cattggagac tgcatcagat tatgtgaaag6420atgacaaagc ttctttctat ggttttatgt gttttgcaat tggatatgaa tttagccctg6480gacctgatga gggagtcatc caattggaaa aagaattagc caatcttggt ggggtttgtg6540cagcagctct gatgaagaag gacctagcac ttcctattgg taatgaatta gaagaagacc6600ttgaaattct tgaggaggct gcattgcagg tgtgcaaaac ccattctgga attcttggaa6660agggtctagc tctttctcat tcaccaacta tattagaagc acttgaggga aatttaccac6720tccaaatcca aagcaatgaa cagtcttttc tggatgattt tattgcctgt gtcccaggat6780caagtggtgg aaggcttgca aggtggcttc agccagattc atatgcggat cctcagaaaa6840catctttgat cctgaataag gatgatattc gttgtggttg gcctaccacc ataactgttc6900aaacaaaaga ccagtatggg gatgtggtac atgttcccaa tatgaaggtg gaagtgaaag6960ctgtccctgt ttctcagaaa aaaatgtctt tacaacaaga tcaagcaaag aaacctcaaa7020ggattcctgg cagtcctgca gtaacagctg catcttctaa tactgacatg acttatggag7080ggctggcatc accaaagcta gatgtttcat atgaaccaat gatagtgaag gaagctcgat7140atattgccat aacaatgatg aaggtttatg aaaattattc atttgaagaa ctacgttttg7200catcaccaac tcctaagaga cccagtgaga atatgctgat ccgtgtcaat aatgatggga7260cttattgtgc aaattggact ccaggggcta ttggactcta cactcttcat gttaccattg7320atggcattga aatcgatgct ggtctggaag taaaagtaaa agacccacca aaagggatga7380taccaccagg aactcagttg gtcaaaccaa agtctgaacc tcagcctaat aaggttcgaa7440aatttgtggc caaggacagt gcggggcttc gcatccgtag ccacccttcc cttcagagtg7500agcagatagg catagtgaaa gtcaatggaa ctatcacttt tattgatgag atccataatg7560atgatggtgt gtggctgagg ctgaatgatg agacaataaa gaagtatgtc cctaacatga7620atggttacac tgaagcctgg tgcctctctt ttaatcaaca tcttggcaag agtcttctgg7680tccctgttga cgaatctaaa actaatactg atgacttttt caaagacata aactcctgct7740
gcccacagga agcaacaatg caagaacaag atatgccatt cttgcgagga gggccaggca7800tgtacaaggt agtgaagacg ggaccttcag gtcacaacat cagaagctgc cctaacctta7860gaggtatccc aattggaatg ttagttctgg gaaacaaagt caaagcagtg ggagaggtaa7920ccaattctga agggacatgg gtgcaactgg atcagaacag catggtagag ttctgtgaga7980gtgatgaagg agaggcatgg tccttagcta gagacagagg cggaaaccag tacctccgac8040atgaagatga acaagctctt ctggatcaga attctcaaac tcctcctcca agccctttct8100cagtgcaagc ttttaataaa ggggcaagtt gcagtgccca aggatttgat tatggactcg8160gaaatagcaa aggtgatcga ggaaacatct caacatcttc taaaccagcc tctacatcag8220gaaaatcaga gctgtcctct aaacacagca gatcgcttaa acctgatgga cgtatgagcc8280ggactactgc tgatcagaag aagccaaggg gcacagaaag tttatctgct agtgaatccc8340tcatcttaaa atctgatgct gcaaagttga ggtcagattc ccacagtagg tcattatccc8400ccaaccataa caccttgcag acattgaaat ctgatgggag gatgccttct agctccagag8460ctgaatcccc aggaccaggt tctcggttgt catctcctaa gccaaagact ctcccagcca8520ataggtctag cccatcgggt gctagttctc cacgctcctc ctcaccacat gataaaaatc8580tacctcaaaa aagtactgct cctgttaaga caaagcttga tcctcctcgg gaacgttcta8640aatcagactc ttacacactt gatccagata ccctccgcaa gaagaaaatg cccctcacag8700aacctttgag aggacggtca acgtcaccaa aaccaaaatc agtaccaaag gattctacag8760attcccctgg atctgaaaat agagctccct ctccccatgt ggtacaggaa aacctccaca8820gtgaggtggt cgaagtctgc acctcaagta ctttaaaaac aaatagtcta acagacagca8880cctgcgatga cagcagtgaa tttaagagtg tggatgaagg ttcaaataaa gttcatttta8940gcattggaaa agcaccactg aaagatgaac aggaaatgag agcatctccc aaaataagtc9000gaaaatgtgc taatagacac accaggccca aaaaagaaaa atcgagtttt cttttcaaag9060gagatggatc caagccttta gagccagcca agcaagccat gtctccttct gtggccgaat9120gtgccagagc tgtgtttgct tccttcctct ggcatgaagg catagtacat gatgcaatgg9180cttgttcttc tttcctaaag tttcatcctg aactttccaa agaacatgct cctataagga9240gtagtttaaa tagccaacaa cctacagaag aaaaagaaac caagttaaaa aatagacatt9300cattagaaat atcatctgca ctgaatatgt ttaatattgc accccatgga ccagatatat9360ctaagatggg tagcatcaac aaaaacaagg tattgtctat gcttaaggaa ccacctctgc9420
atgaaaaatg tgaggatggg aaaaccgaga ccacttttga aatgtccatg cataacacaa9480tgaagtctaa gtctcctctt cccttaactt tacaacattt agtggctttt tgggaagaca9540tctctttggc tactatcaaa gctgcttccc agaatatgat ttttccaagt cctggttcct9600gtgcagttct taaaaagaaa gagtgtgaga aaggaaggaa taagaagtcc aaaaaggaaa9660aaaagaaaaa agaaaaggca gaagttaggc ccaggggtaa tttgtttgga gagatggccc9720agctggcagt aggaggacca gagaaagata ccatctgtga actgtgtggg gagtcacatc9780catacccggt gacctatcac atgagacaag ctcacccagg ttgtggccga tatgctggtg9840gacaaggtta caatagcatt gggcattttt gtggaggatg ggctggtaac tgtggtgatg9900gtggcatagg aggaagcact tggtatctgg tatgtgatcg ctgtagagaa aaatacctcc9960gcgaaaaaca ggctgctgca agggagaagg tcaaacaatc taggagaaaa ccaatgcaag10020tcaagacccc tcgtgccttg cccaccatgg aagctcacca ggtgattaaa gccaatgcac10080tcttcctgct gtccctgagc agtgcagcag aaccgagcat tctgtgttac catcctgcaa10140agccattcca atctcagttg cccagtgtaa aagaaggcat ttctgaggat cttcctgtga10200aaatgccttg tctgtacctg cagacattag ctaggcatca tcatgaaaat tttgtgggct10260atcaagatga caatctattc caggatgaaa tgagatatct acgttcaaca tctgtacctg10320ccccgtatat atcagtaact cctgatgcaa gtcctaatgt atttgaagag ccagagagca10380atatgaagtc tatgccacca agtttagaaa ccagtcccat aactgatact gatcttgcaa10440agagaactgt cttccaaaga tcatactcag ttgttgcttc cgaatatgat aaacaacact10500ccattttacc tgcacgagtt aaagctattc ctagaagaag agttaacagt ggagacactg10560aagttggttc ttcccttttg agacatccgt ctcctgagct ttctcggcta atctcagccc10620acagctctct ttctaaagga gaacgaaatt tccagtggcc agttttagct tttgttatac10680aacatcatga tctagaaggt cttgaaatag caatgaaaca ggccctaagg aaatctgctt10740gtcgagtttt tgctatggag gctttcaact ggcttctgtg taatgtcatc caaaccactt10800ctctccatga tattctgtgg cattttgtgg catcactgac tcctgcacca gtggaaccag10860aggaagaaga ggatgaagaa aataaaacaa gcaaagaaaa ttcagaacaa gagaaagata10920caagagtatg tgaacatcca ctctcagaca tagtgattgc cggggaacgt gctcatcctt10980taccacacac ctttcaccgc ttgctgcaga ccatctcaga ccttatgatg tctctcccca11040gcggcagttc attacagcaa atggccctga ggtgctggag tctcaaattc aagcaatctg11100
atcaccagtt ccttcatcag agcaacgtct ttcatcacat taacaatatt ttgtcaaagt 11160cagatgatgg cgatagtgaa gagagtttta gcatcagtat acagtctggc tttgaagcta 11220tgagtcagga attatgcata gtaatgtgct taaaggactt aaccagcatt gttgacataa 11280aaacttcaag ccgacctgcc atgattggca gtttgacaga cggctccaca gaaacctttt 11340gggaatcagg agatgaagat aaaaacaaaa ctaagaacat caccatcaac tgtgtaaaag 11400gaatcaatgc ccgctatgtg tctgttcacg tggacaattc ccgagatctt gggaataaag 11460ttacctcaat gaccttctta actggcaaag cagtagaaga tttgtgcaga ataaagcagg 11520ttgatctgga ttccaggcac attggctggg taacaagtga acttccagga ggggataatc 11580acatcataaa aattgaatta aaaggcccag aaaatacact gagagttcga caagtcaaag 11640tcctgggctg gaaagatggt gaaagcacaa aaatagctgg ccagatttca gccagtgtgg 11700cccagcagag gaactgtgaa gctgagactc tgcgagtatt cagactgatt acgtctcaag 11760tatttggaaa gctcatctct ggagatgctg aacctacacc agaacaagag gaaaaagcac 11820tattgtcatc acctgaagga gaagaaaaag tatacaatgc aacatcagat gctgacctga 11880aagaacatat ggttggaatc atattcagca ggagtaagct gactaactta caaaaacagg 11940tgtgtgctca tattgtccaa gctattcgca tggaagctac cagagtccgt gaagaatggg 12000aacatgctat atcaagcaaa gaaaatgcca attctcagcc aaatgatgaa gatgcctcct 12060ctgatgccta ctgctttgag ctgctctcta tggttttagc actgagtggc tctaacgttg 12120gccggcaata tctggctcaa cagctaaccc tgcttcagga tctcttctcg ctgcttcaca 12180cagcctctcc tagagtccag agacaggtaa cctctttact aagaagagtt ttgcctgaag 12240taacccctag tcgtctggcc agcatcatag gagtgaaatc cctcccccca gcagatatca 12300gtgatatcat tcactcaaca gagaaaggag actggaataa gctgggtatc ttggacatgt 12360ttctaggatg cattgccaaa gcactcactg tacagctaaa agccaaagga accaccatca 12420ctggaacagc tggtaccact gtgggcaaag gagttacaac agttactctt ccgatgattt 12480tcaattccag ttatctccga cgaggtgaaa gtcattggtg gatgaagggc tcaaccccta 12540cccagatctc agagatcatc attaaactta tcaaggatat ggcagcaggt catctgtcag 12600aagcttggtc ccgagtgaca aaaaatgcta ttgcagaaac catcattgcc ttgaccaaga 12660tggaagaaga atttaggtct ccagtgagat gtattgcaac aactagactc tggcttgctc 12720tcgcatccct atgtgttctt gatcaggacc acgtagatcg tctctcctcg gggagatgga 12780
tgggaaagga tggacaacaa aaacaaatgc ctatgtgtga taaccatgat gatggtgaaa 12840ctgcagcaat cattttatgc aatgtctgtg gaaatttatg tacagactgt gacagattcc 12900ttcaccttca tcgaagaacc aaaactcatc aaagacaggt cttcaaagaa gaagaagaag 12960ctataaaggt tgaccttcat gaaggttgtg gtagaaccaa attgttctgg ttgatggcac 13020tggcagattc taaaacaatg aaggcaatgg tggaattccg agaacacaca ggcaaaccca 13080ccacgagtag ctcagaagca tgtcgcttct gtggttccag gagtggaaca gagttatctg 13140ctgttggcag tgtttgttct gatgcagatt gccaggaata cgctaagata gcctgtagta 13200agacgcatcc ttgtggccat ccatgcgggg gtgttaaaaa cgaagagcac tgtctgccct 13260gtctacacgg ctgtgacaaa agtgccacaa gcctgaagca agacgccgat gacatgtgca 13320tgatatgttt caccgaagcg ctctcggcag caccagccat tcagctggat tgtagtcaca 13380tattccactt acagtgctgt cggcgagtat tagaaaatcg atggcttggc ccaaggataa 13440catttggatt tatatcttgt cccatttgca agaacaaaat taatcacata gtactaaaag 13500acctacttga tccaataaaa gaactctatg aggatgtcag aagaaaagcc ttaatgagat 13560tggaatatga aggtctgcat aagagtgaag ctatcacaac tcctggtgtg aggttttata 13620atgacccagc tggctatgca atgaatagat atgcatatta tgtgtgctac aaatgcagaa 13680aggcatattt tggtggtgaa gctcgctgcg atgctgaggc tggacgggga gatgattatg 13740atcccagaga gctcatttgt ggtgcctgtt ctgatgtttc cagggctcag atgtgtccca 13800aacatggcac agactttttg gaatataaat gtcgctactg ctgttcagtg gctgtttttt 13860tctgttttgg aacaacacat ttttgtaatg cttgtcatga tgattttcaa agaatgacta 13920gcattcctaa ggaagaacta ccacactgtc ctgcaggtcc caaaggcaag cagttagaag 13980gaactgaatg tccactccat gttgttcatc cacccactgg ggaagagttt gctctgggat 14040gtggagtgtg cagaaatgcc cacacttttt agaacacgca gatcctttgt ctacagagag 14100aaaaattgcc ttcatccccc aagaggatgc ggtgaagttt aaactctgct caccataagg 14160acgggaccat ttttacatcc atgaaaatga accattcaca gtgcaagaag gataccaaat 14220accatgtaca taattcttgc tatgaaaagt ttccccatta ttttggttta tcttcttttg 14280aacaaatgac atcaaacttg tgaggtgttt gcatgtggcc attaccgtca ttggcctgtg 14340aagcattgga catttataga taattgatat aaaagaatcg ccatgcccat ggactaagaa 14400cgatgctggc tttcaagcaa aaaagaaaaa taatcattgt ttattgtata ctgccttttt 14460
gtaatcctgt acaattgcat cacgggtggg gataaaaaga ggaatattct ggtttatttc 14520ctagactgtt atttaaaaaa aaaaaaaaca ttgtgttagg acagcatata aatgtaataa 14580gtatcacact gtatataaac atatcaatgt ttgtcctgta taagaattac taaattacaa 14640atgcaatttc atttaaactt ctaggttaag tttgagcctg aaattttaat gaagtgcaat 14700actgagtgtg cctcattatc ttgcagctgt aaacatattg gaatgtacat gtcaataaaa 14760ccactgtaca tttttataca gtgataaagt ctaaaaaaaa aaaaaaa14807<210>2<211>4641<212>PRT<213>人<400>2Met Pro Val Pro Asp Gly Ser Val Ala Ala Ala Gly Leu Gly Leu Gly1 5 10 15Leu Pro Ala Ala Asp Ser Pro Gly His Tyr Gln Leu Leu Leu Ser Gly20 25 30Arg Ala Leu Ala Asp Arg Tyr Arg Arg Ile Tyr Thr Ala Ala Leu Asn35 40 45Asp Arg Asp Gln Gly Gly Gly Ser Ala Gly His Pro Ala Ser Arg Asn50 55 60Lys Lys Ile Leu Asn Lys Lys Lys Leu Lys Arg Lys Gln Lys Ser Lys65 70 75 80Ser Lys Val Lys Thr Arg Ser Lys Ser Glu Asn Leu Glu Asn Thr Val85 90 95Ile Ile Pro Asp Ile Lys Leu His Ser Asn Pro Ser Ala Phe Asn Ile100 105 110Tyr Cys Asn Val Arg His Cys Val Leu Glu Trp Gln Lys Lys Glu Ile115 120 125Ser Leu Ala Ala Ala Ser Lys Asn Ser Val Gln Ser Gly Glu Ser Asp130 135 140
Ser Asp Glu Glu Glu Glu Ser Lys Glu Pro Pro Ile Lys Leu Pro Lys145 150 155 160Ile Ile Glu Val Gly Leu Cys Glu Val Phe Glu Leu Ile Lys Glu Thr165 170 175Arg Phe Ser His Pro Ser Leu Cys Leu Arg Ser Leu Gln Ala Leu Leu180 185 190Asn Val Leu Gln Gly Gln Gln Pro Glu Val Leu Gln Ser Glu Pro Pro195 200 205Glu Val Leu Glu Ser Leu Phe Gln Leu Leu Leu Glu Ile Thr Val Arg210 215 220Ser Thr Gly Met Asn Asp Ser Thr Gly Gln Ser Leu Thr Ala Leu Ser225 230 235 240Cys Ala Cys Leu Phe Ser Leu Val Ala Ser Trp Gly Glu Thr Gly Arg245 250 255Thr Leu Gln Ala Ile Ser Ala Ile Leu Thr Asn Asn Gly Ser His Ala260 265 270Cys Gln Thr Ile Gln Val Pro Thr Ile Leu Asn Ser Leu Gln Arg Ser275 280 285Val Gln Ala Val Leu Val Gly Lys Ile Gln Ile Gln Asp Trp Phe Ser290 295 300Asn Gly Ile Lys Lys Ala Ala Leu Met His Lys Trp Pro Leu Lys Glu305 310 315 320Ile Ser Val Asp Glu Asp Asp Gln Cys Leu Leu Gln Asn Asp Gly Phe325 330 335Phe Leu Tyr Leu Leu Cys Lys Asp Gly Leu Tyr Lys Ile Gly Ser Gly340 345 350Tyr Ser Gly Thr Val Arg Gly His Ile Tyr Asn Ser Thr Ser Arg Ile355 360 365
Arg Asn Arg Lys Glu Lys Lys Ser Trp Leu Gly Tyr Ala Gln Gly Tyr370 375 380Leu Leu Tyr Arg Asp Val Asn Asn His Ser Met Thr Ala Ile Arg Ile385 390 395 400Ser Pro Glu Thr Leu Glu Gln Asp Gly Thr Val Met Leu Pro Asp Cys405 410 415His Thr Glu Gly Gln Asn Ile Leu Phe Thr Asp Gly Glu Tyr Ile Asn420 425 430Gln Ile Ala Ala Ser Arg Asp Asp Gly Phe Val Val Arg Ile Phe Ala435 440 445Thr Ser Thr Glu Pro Val Leu Gln Gln Glu Leu Gln Leu Lys Leu Ala450 455 460Arg Lys Cys Leu His Ala Cys Arg Ile Ser Leu Phe Asp Leu Glu Lys465 470 475 480Asp Leu His Ile Ile Ser Thr Gly Phe Asp Glu Glu Ser Ala Ile Leu485 490 495Gly Ala Gly Arg Glu Phe Ala Leu Met Lys Thr Ala Asn Gly Lys Ile500 505 510Tyr Tyr Thr Gly Lys Tyr Gln Ser Leu Gly Ile Lys Gln Gly Gly Pro515 520 525Ser Ala Gly Lys Trp Val Glu Leu Pro Ile Thr Lys Ser Pro Lys Ile530 535 540Val His Phe Ser Val Gly His Asp Gly Ser His Ala Leu Leu Val Ala545 550 555 560Glu Asp Gly Ser Ile Phe Phe Thr Gly Ser Ala Ser Lys Gly Glu Asp565 570 575Gly Glu Ser Ile Lys Ser Arg Arg Gln Ser Lys Pro Tyr Lys Pro Lys580 585 590
Lys Ile Ile Lys Met Glu Gly Lys Ile Val Val Tyr Thr Ala Cys Asn595 600 605Asn Gly Ser Ser Ser Val Ile Ser Lys Asp Gly Glu Leu Tyr Met Phe610 615 620Gly Lys Asp Ala Ile Tyr Ser Asp Ser Ser Ser Leu Val Thr Asp Leu625 630 635 640Lys Gly His Phe Val Thr Gln Val Ala Met Gly Lys Ala His Thr Cys645 650 655Val Leu Met Lys Asn Gly Glu Val Trp Thr Phe Gly Val Asn Asn Lys660 665 670Gly Gln Cys Gly Arg Asp Thr Gly Ala Met Asn Gln Gly Gly Lys Gly675 680 685Phe Gly Val Glu Asn Met Ala Thr Ala Met Asp Glu Asp Leu Glu Glu690 695 700Glu Leu Asp Glu Lys Asp Glu Lys Ser Met Met Cys Pro Pro Gly Met705 710 715 720His Lys Trp Lys Leu Glu Gln Cys Met Val Cys Thr Val Cys Gly Asp725 730 735Cys Thr Gly Tyr Gly Ala Ser Cys Val Ser Ser Gly Arg Pro Asp Arg740 745 750Val Pro Gly Gly Ile Cys Gly Cys Gly Ser Gly Glu Ser Gly Cys Ala755 760 765Val Cys Gly Cys Cys Lys Ala Cys Ala Arg Glu Leu Asp Gly Gln Glu770 775 780Ala Arg Gln Arg Gly Ile Leu Asp Ala Val Lys Glu Met Ile Pro Leu785 790 795 800Asp Leu Leu Leu Ala Val Pro Val Pro Gly Val Asn Ile Glu Glu His805 810 815
Leu Gln Leu Arg Gln Glu Glu Lys Arg Gln Arg Val Ile Arg Arg His820 825830Arg Leu Glu Glu Gly Arg Gly Pro Leu Val Phe Ala Gly Pro Ile Phe835 840 845Met Asn His Arg Glu Gln Ala Leu Ala Arg Leu Arg Ser His Pro Ala850 855 860His Val Lys His Lys Arg Asp Lys His Lys Asp Gly Ser Gly Glu Arg865 870 875 880Gly Glu Lys Asp Ala Ser Lys Ile Thr Thr Tyr Pro Pro Gly Ser Val885 890 895Arg Phe Asp Cys Glu Leu Arg Ala Val Gln Val Ser Cys Gly Phe His900 905 910His Ser Val Val Leu Met Glu Asn Gly Asp Val Tyr Thr Phe Gly Tyr915 920 925Gly Gln His Gly Gln Leu Gly His Gly Asp Val Asn Ser Arg Gly Cys930 935 940Pro Thr Leu Val Gln Ala Leu Pro Gly Pro Ser Thr Gln Val Thr Ala945 950 955 960Gly Ser Asn His Thr Ala Val Leu Leu Met Asp Gly Gln Val Phe Thr965 970 975Phe Gly Ser Phe Ser Lys Gly Gln Leu Gly Arg Pro Ile Leu Asp Val980 985 990Pro Tyr Trp Asn Ala Lys Pro Ala Pro Met Pro Asn Ile Gly Ser Lys99510001005Tyr Gly Arg Lys Ala Thr Trp Ile Gly Ala Ser Gly Asp Gln Thr101010151020Phe Leu Arg Ile Asp Glu Ala Leu Ile Asn Ser His Val Leu Ala102510301035
Thr Ser Glu Ile Phe Ala Ser Lys His Ile Ile Gly Leu Val Pro104010451050Ala Ser Ile Ser Glu Pro Pro Pro Phe Lys Cys Leu Leu Ile Asn105510601065Lys Val Asp Gly Ser Cys Lys Thr Phe Asn Asp Ser Glu Gln Glu107010751080Asp Leu Gln Gly Phe Gly Val Cys Leu Asp Pro Val Tyr Asp Val108510901095Ile Trp Arg Phe Arg Pro Asn Thr Arg Glu Leu Trp Cys Tyr Asn110011051110Ala Val Val Ala Asp Ala Arg Leu Pro Ser Ala Ala Asp Met Gln111511201125Ser Arg Cys Ser Ile Leu Ser Pro Glu Leu Ala Leu Pro Thr Gly113011351140Ser Arg Ala Leu Thr Thr Arg Ser His Ala Ala Leu His Ile Leu114511501155Gly Cys Leu Asp Thr Leu Ala Ala Met Gln Asp Leu Lys Met Gly116011651170Val Ala Ser Thr Glu Glu Glu Thr Gln Ala Val Met Lys Val Tyr117511801185Ser Lys Glu Asp Tyr Ser Val Val Asn Arg Phe Glu Ser His Gly119011951200Gly Gly Trp Gly Tyr Ser Ala His Ser Val Glu Ala Ile Arg Phe120512101215Ser Ala Asp Thr Asp Ile Leu Leu Gly Gly Leu Gly Leu Phe Gly122012251230Gly Arg Gly Glu Tyr Thr Ala Lys Ile Lys Leu Phe Glu Leu Gly123512401245
Pro Asp Gly Gly Asp His Glu Thr Asp Gly Asp Leu Leu Ala Glu125012551260Thr Asp Val Leu Ala Tyr Asp Cys Ala Ala Arg Glu Lys Tyr Ala126512701275Met Met Phe Asp Glu Pro Val Leu Leu Gln Ala Gly Trp Trp Tyr128012851290Val Ala Trp Ala Arg Val Ser Gly Pro Ser Ser Asp Cys Gly Ser129513001305His Gly Gln Ala Ser Ile Thr Thr Asp Asp Gly Val Val Phe Gln131013151320Phe Lys Ser Ser Lys Lys Ser Asn Asn Gly Thr Asp Val Asn Ala132513301335Gly Gln Ile Pro Gln Leu Leu Tyr Arg Leu Pro Thr Ser Asp Gly134013451350Ser Ala Ser Lys Gly Lys Gln Gln Thr Ser Glu Pro Val His Ile135513601365Leu Lys Arg Ser Phe Ala Arg Thr Val Ser Val Glu Cys Phe Glu137013751380Ser Leu Leu Ser Ile Leu His Trp Ser Trp Thr Thr Leu Val Leu138513901395Gly Val Glu Glu Leu Arg Gly Leu Lys Gly Phe Gln Phe Thr Ala140014051410Thr Leu Leu Asp Leu Glu Arg Leu Arg Phe Val Gly Thr Cys Cys141514201425Leu Arg Leu Leu Arg Val Tyr Thr Cys Glu Ile Tyr Pro Val Ser143014351440Ala Thr Gly Lys Ala Val Val Glu Glu Thr Ser Lys Leu Ala Glu144514501455
Cys Ile Gly Lys Thr Arg Thr Leu Leu Arg Lys Ile Leu Ser Glu146014651470Pro Leu Asp His Cys Met Val Lys Leu Asp Asn Asp Pro Gln Gly147514801485Tyr Leu Ser Gln Pro Leu Ser Leu Leu Glu Ala Val Leu Gln Glu149014951500Cys His Asn Thr Phe Thr Ala Cys Phe His Ser Phe Tyr Pro Thr150515101515Pro Ala Leu Gln Trp Ala Cys Leu Cys Asp Leu Leu Asn Cys Leu152015251530Asp Gln Asp Ile Gln Glu Ala Asn Phe Lys Thr Ser Ser Ser Arg153515401545Leu Leu Ala Ala Val Met Ser Ala Leu Cys His Thr Ser Val Lys155015551560Leu Thr Ser Ile Phe Pro Ile Ala Tyr Asp Gly Glu Val Leu Leu156515701575Arg Ser Ile Val Lys Gln Val Ser Thr Glu Asn Asp Ser Thr Leu158015851590Val His Arg Phe Pro Leu Leu Val Ala His Met Glu Lys Leu Ser159516001605Gln Ser Glu Glu Asn Ile Ser Gly Met Thr Ser Phe Arg Glu Val161016151620Leu Glu Lys Met Leu Val Ile Val Val Leu Pro Val Arg Asn Ser162516301635Leu Arg Arg Glu Asn Glu Leu Phe Ser Ser His Leu Val Ser Asn164016451650Thr Cys Gly Leu Leu Ala Ser Ile Val Ser Glu Leu Thr Ala Ser165516601665
Ala Leu Gly Ser Glu Val Asp Gly Leu Asn Ser Leu His Ser Val167016751680Lys Ala Ser Ala Asn Arg Phe Thr Lys Thr Ser Gln Gly Arg Ser168516901695Trp Asn Thr Gly Asn Gly Ser Pro Asp Ala Ile Cys Phe Ser Val170017051710Asp Lys Pro Gly Ile Val Val Val Gly Phe Ser Val Tyr Gly Gly171517201725Gly Gly Ile His Glu Tyr Glu Leu Glu Val Leu Val Asp Asp Ser173017351740Glu His Ala Gly Asp Ser Thr His Ser His Arg Trp Thr Ser Leu174517501755Glu Leu Val Lys Gly Thr Tyr Thr Thr Asp Asp Ser Pro Ser Asp176017651770Ile Ala GluIle Arg Leu Asp Lys Val Val Pro Leu Lys Glu Asn177517801785Val Lys Tyr Ala Val Arg Leu Arg Asn Tyr Gly Ser Arg Thr Ala179017951800Asn Gly Asp Gly Gly Met Thr Thr Val Gln Cys Pro Asp Gly Val180518101815Thr Phe Thr Phe Ser Thr Cys Ser Leu Ser Ser Asn Gly Thr Asn182018251830Gln Thr Arg Gly Gln Ile Pro Gln Ile Leu Tyr Tyr Arg Ser Glu183518401845Phe Asp Gly Asp Leu Gln Ser Gln Leu Leu Ser Lys Ala Asn Glu185018551860Glu Asp Lys Asn Cys Ser Arg Ala Leu Ser Val Val Ser Thr Val186518701875
Val Arg Ala Ser Lys Asp Leu Leu His Arg Ala Leu Ala Val Asp1880 1885 1890Ala Asp Asp Ile Pro Glu Leu Leu Ser Ser Ser Ser Leu Phe Ser1895 1900 1905Met Leu Leu Pro Leu Ile Ile Ala Tyr Ile Gly Pro Val Ala Ala1910 1915 1920Ala Ile Pro Lys Val Ala Val Glu Val Phe Gly Leu Val Gln Gln1925 1930 1935Leu Leu Pro Ser Val Ala Ile Leu Asn Gln Lys Tyr Ala Pro Pro1940 1945 1950Ala Phe Asn Pro Asn Gln Ser Thr Asp Ser Thr Thr Gly Asn Gln1955 1960 1965Pro Glu Gln Gly Leu Ser Ala Cys Thr Thr Ser Ser His Tyr Ala1970 1975 1980Val Ile Glu Ser Glu His Pro Tyr Lys Pro Ala Cys Val Met His1985 1990 1995Tyr Lys Val Thr Phe Pro Glu Cys Val Arg Trp Met Thr Ile Glu2000 2005 2010Phe Asp Pro Gln Cys Gly Thr Ala Gln Ser Glu Asp Val Leu Arg2015 2020 2025Leu Leu Ile Pro Val Arg Thr Val Gln Asn Ser Gly Tyr Gly Pro2030 2035 2040Lys Leu Thr Ser Val His Glu Asn Leu Asn Ser Trp Ile Glu Leu2045 2050 2055Lys Lys Phe Ser Gly Ser Ser Gly Trp Pro Thr Met Val Leu Val2060 2065 2070Leu Pro Gly Asn Glu Ala Leu Phe Ser Leu Glu Thr Ala Ser Asp2075 2080 2085
Tyr Val Lys Asp Asp Lys Ala Ser Phe Tyr Gly Phe Met Cys Phe2090 2095 2100Ala Ile Gly Tyr Glu Phe Ser Pro Gly Pro Asp Glu Gly Val Ile2105 2110 2115Gln Leu Glu Lys Glu Leu Ala Asn Leu Gly Gly Val Cys Ala Ala2120 2125 2130Ala Leu Met Lys Lys Asp Leu Ala Leu Pro Ile Gly Asn Glu Leu2135 2140 2145Glu Glu Asp Leu Glu Ile Leu Glu Glu Ala Ala Leu Gln Val Cys2150 2155 2160Lys Thr His Ser Gly Ile Leu Gly Lys Gly Leu Ala Leu Ser His2165 2170 2175Ser Pro Thr Ile Leu Glu Ala Leu Glu Gly Asn Leu Pro Leu Gln2180 2185 2190Ile Gln Ser Asn Glu Gln Ser Phe Leu Asp Asp Phe Ile Ala Cys2195 2200 2205Val Pro Gly Ser Ser Gly Gly Arg Leu Ala Arg Trp Leu Gln Pro2210 2215 2220Asp Ser Tyr Ala Asp Pro Gln Lys Thr Ser Leu Ile Leu Asn Lys2225 2230 2235Asp Asp Ile Arg Cys Gly Trp Pro Thr Thr Ile Thr Val Gln Thr2240 2245 2250Lys Asp Gln Tyr Gly Asp Val Val His Val Pro Asn Met Lys Val2255 2260 2265Glu Val Lys Ala Val Pro Val Ser Gln Lys Lys Met Ser Leu Gln2270 2275 2280Gln Asp Gln Ala Lys Lys Pro Gln Arg Ile Pro Gly Ser Pro Ala2285 2290 2295
Val Thr Ala Ala Ser Ser Asn Thr Asp Met Thr Tyr Gly Gly Leu2300 2305 2310Ala Ser Pro Lys Leu Asp Val Ser Tyr Glu Pro Met Ile Val Lys2315 2320 2325Glu Ala Arg Tyr Ile Ala Ile Thr Met Met Lys Val Tyr Glu Asn2330 2335 2340Tyr Ser Phe Glu Glu Leu Arg Phe Ala Ser Pro Thr Pro Lys Arg2345 2350 2355Pro Ser Glu Asn Met Leu Ile Arg Val Asn Asn Asp Gly Thr Tyr2360 2365 2370Cys Ala Asn Trp Thr Pro Gly Ala Ile Gly Leu Tyr Thr Leu His2375 2380 2385Val Thr Ile Asp Gly Ile Glu Ile Asp Ala Gly Leu Glu Val Lys2390 2395 2400Val Lys Asp Pro Pro Lys Gly Met Ile Pro Pro Gly Thr Gln Leu2405 2410 2415Val Lys Pro Lys Ser Glu Pro Gln Pro Asn Lys Val Arg Lys Phe2420 2425 2430Val Ala Lys Asp Ser Ala Gly Leu Arg Ile Arg Ser His Pro Ser2435 2440 2445Leu Gln Ser Glu Gln Ile Gly Ile Val Lys Val Asn Gly Thr Ile2450 2455 2460Thr Phe Ile Asp Glu Ile His Asn Asp Asp Gly Val Trp Leu Arg2465 2470 2475Leu Asn Asp Glu Thr Ile Lys Lys Tyr Val Pro Asn Met Asn Gly2480 2485 2490Tyr Thr Glu Ala Trp Cys Leu Ser Phe Asn Gln His Leu Gly Lys2495 2500 2505
Ser Leu Leu Val Pro Val Asp Glu Ser Lys Thr Asn Thr Asp Asp2510 2515 2520Phe Phe Lys Asp Ile Asn Ser Cys Cys Pro Gln Glu Ala Thr Met2525 2530 2535Gln Glu Gln Asp Met Pro Phe Leu Arg Gly Gly Pro Gly Met Tyr2540 2545 2550Lys Val Val Lys Thr Gly Pro Ser Gly His Asn Ile Arg Ser Cys2555 2560 2565Pro Asn Leu Arg Gly Ile Pro Ile Gly Met Leu Val Leu Gly Asn2570 2575 2580Lys Val Lys Ala Val Gly Glu Val Thr Asn Ser Glu Gly Thr Trp2585 2590 2595Val Gln Leu Asp Gln Asn Ser Met Val Glu Phe Cys Glu Ser Asp2600 2605 2610Glu Gly Glu Ala Trp Ser Leu Ala Arg Asp Arg Gly Gly Asn Gln2615 2620 2625Tyr Leu Arg His Glu Asp Glu Gln Ala Leu Leu Asp Gln Asn Ser2630 2635 2640Gln Thr Pro Pro Pro Ser Pro Phe Ser Val Gln Ala Phe Asn Lys2645 2650 2655Gly Ala Ser Cys Ser Ala Gln Gly Phe Asp Tyr Gly Leu Gly Asn2660 2665 2670Ser Lys Gly Asp Arg Gly Asn Ile Ser Thr Ser Ser Lys Pro Ala2675 2680 2685Ser Thr Ser Gly Lys Ser Glu Leu Ser Ser Lys His Ser Arg Ser2690 2695 2700Leu Lys Pro Asp Gly Arg Met Ser Arg Thr Thr Ala Asp Gln Lys2705 2710 2715
Lys Pro Arg Gly Thr Glu Ser Leu Ser Ala Ser Glu Ser Leu Ile2720 2725 2730Leu Lys Ser Asp Ala Ala Lys Leu Arg Ser Asp Ser His Ser Arg2735 2740 2745Ser Leu Ser Pro Asn His Asn Thr Leu Gln Thr Leu Lys Ser Asp2750 2755 2760Gly Arg Met Pro Ser Ser Ser Arg Ala Glu Ser Pro Gly Pro Gly2765 2770 2775Ser Arg Leu Ser Ser Pro Lys Pro Lys Thr Leu Pro Ala Asn Arg2780 2785 2790Ser Ser Pro Ser Gly Ala Ser Ser Pro Arg Ser Ser Ser Pro His2795 2800 2805Asp Lys Asn Leu Pro Gln Lys Ser Thr Ala Pro Val Lys Thr Lys2810 2815 2820Leu Asp Pro Pro Arg Glu Arg Ser Lys Ser Asp Ser Tyr Thr Leu2825 2830 2835Asp Pro Asp Thr Leu Arg Lys Lys Lys Met Pro Leu Thr Glu Pro2840 2845 2850Leu Arg Gly Arg Ser Thr Ser Pro Lys Pro Lys Ser Val Pro Lys2855 2860 2865Asp Ser Thr Asp Ser Pro Gly Ser Glu Asn Arg Ala Pro Ser Pro2870 2875 2880His Val Val Gln Glu Asn Leu His Ser Glu Val Val Glu Val Cys2885 2890 2895Thr Ser Ser Thr Leu Lys Thr Asn Ser Leu Thr Asp Ser Thr Cys2900 2905 2910Asp Asp Ser Ser Glu Phe Lys Ser Val Asp Glu Gly Ser Asn Lys2915 2920 2925
Val His Phe Ser Ile Gly Lys Ala Pro Leu Lys Asp Glu Gln Glu2930 2935 2940Met Arg Ala Ser Pro Lys Ile Ser Arg Lys Cys Ala Asn Arg His2945 2950 2955Thr Arg Pro Lys Lys Glu Lys Ser Ser Phe Leu Phe Lys Gly Asp2960 2965 2970Gly Ser Lys Pro Leu Glu Pro Ala Lys Gln Ala Met Ser Pro Ser2975 2980 2985Val Ala Glu Cys Ala Arg Ala Val Phe Ala Ser Phe Leu Trp His2990 2995 3000Glu Gly Ile Val His Asp Ala Met Ala Cys Ser Ser Phe Leu Lys3005 3010 3015Phe His Pro Glu Leu Ser Lys Glu His Ala Pro Ile Arg Ser Ser3020 3025 3030Leu Asn Ser Gln Gln Pro Thr Glu Glu Lys Glu Thr Lys Leu Lys3035 3040 3045Asn Arg His Ser Leu Glu Ile Ser Ser Ala Leu Asn Met Phe Asn3050 3055 3060Ile Ala Pro His Gly Pro Asp Ile Ser Lys Met Gly Ser Ile Asn3065 3070 3075Lys Asn Lys Val Leu Ser Met Leu Lys Glu Pro Pro Leu His Glu3080 3085 3090Lys Cys Glu Asp Gly Lys Thr Glu Thr Thr Phe Glu Met Ser Met3095 3100 3105His Asn Thr Met Lys Ser Lys Ser Pro Leu Pro Leu Thr Leu Gln3110 3115 3120His Leu Val Ala Phe Trp Glu Asp Ile Ser Leu Ala Thr Ile Lys3125 3130 3135
Ala Ala Ser Gln Asn Met Ile Phe Pro Ser Pro Gly Ser Cys Ala3140 3145 3150Val Leu Lys Lys Lys Glu Cys Glu Lys Gly Arg Asn Lys Lys Ser3155 3160 3165Lys Lys Glu Lys Lys Lys Lys Glu Lys Ala Glu Val Arg Pro Arg3170 3175 3180Gly Asn Leu Phe Gly Glu Met Ala Gln Leu Ala Val Gly Gly Pro3185 3190 3195Glu Lys Asp Thr Ile Cys Glu Leu Cys Gly Glu Ser His Pro Tyr3200 3205 3210Pro Val Thr Tyr His Met Arg Gln Ala His Pro Gly Cys Gly Arg3215 3220 3225Tyr Ala Gly Gly Gln Gly Tyr Asn Ser Ile Gly His Phe Cys Gly3230 3235 3240Gly Trp Ala Gly Asn Cys Gly Asp Gly Gly Ile Gly Gly 5er Thr3245 3250 3255Trp Tyr Leu Val Cys Asp Arg Cys Arg Glu Lys Tyr Leu Arg Glu3260 3265 3270Lys Gln Ala Ala Ala Arg Glu Lys Val Lys Gln Ser Arg Arg Lys3275 3280 3285Pro Met Gln Val Lys Thr Pro Arg Ala Leu Pro Thr Met Glu Ala3290 3295 3300His Gln Val Ile Lys Ala Asn Ala Leu Phe Leu Leu Ser Leu Ser3305 3310 3315Ser Ala Ala Glu Pro Ser Ile Leu Cys Tyr His Pro Ala Lys Pro3320 3325 3330Phe Gln Ser Gln Leu Pro Ser Val Lys Glu Gly Ile Ser Glu Asp3335 3340 3345
Leu Pro Val Lys Met Pro Cys Leu Tyr Leu Gln Thr Leu Ala Arg3350 3355 3360His His His Glu Asn Phe Val Gly Tyr Gln Asp Asp Asn Leu Phe3365 3370 3375Gln Asp Glu Met Arg Tyr Leu Arg Ser Thr Ser Val Pro Ala Pro3380 3385 3390Tyr Ile Ser Val Thr Pro Asp Ala Ser Pro Asn Val Phe Glu Glu3395 3400 3405Pro Glu Ser Asn Met Lys Ser Met Pro Pro Ser Leu Glu Thr Ser3410 3415 3420Pro Ile Thr Asp Thr Asp Leu Ala Lys Arg Thr Val Phe Gln Arg3425 3430 3435Ser Tyr Ser Val Val Ala Ser Glu Tyr Asp Lys Gln His Ser Ile3440 3445 3450Leu Pro Ala Arg Val Lys Ala Ile Pro Arg Arg Arg Val Asn Ser3455 3460 3465Gly Asp Thr Glu Val Gly Ser Ser Leu Leu Arg His Pro Ser Pro3470 3475 3480Glu Leu Ser Arg Leu Ile Ser Ala His Ser Ser Leu Ser Lys Gly3485 3490 3495Glu Arg Asn Phe Gln Trp Pro Val Leu Ala Phe Val Ile Gln His3500 3505 3510His Asp Leu Glu Gly Leu Glu Ile Ala Met Lys Gln Ala Leu Arg3515 3520 3525Lys Ser Ala Cys Arg Val Phe Ala Met Glu Ala Phe Asn Trp Leu3530 3535 3540Leu Cys Asn Val Ile Gln Thr Thr Ser Leu His Asp Ile Leu Trp3545 3550 3555
His Phe Val Ala Ser Leu Thr Pro Ala Pro Val Glu Pro Glu Glu3560 3565 3570Glu Glu Asp Glu Glu Asn Lys Thr Ser Lys Glu Asn Ser Glu Gln3575 3580 3585Glu Lys Asp Thr Arg Val Cys Glu His Pro Leu Ser Asp Ile Val3590 3595 3600Ile Ala Gly Glu Arg Ala His Pro Leu Pro His Thr Phe His Arg3605 3610 3615Leu Leu Gln Thr Ile Ser Asp Leu Met Met Ser Leu Pro Ser Gly3620 3625 3630Ser Ser Leu Gln Gln Met Ala Leu Arg Cys Trp Ser Leu Lys Phe3635 3640 3645Lys Gln Ser Asp His Gln Phe Leu His Gln Ser Asn Val Phe His3650 3655 3660His Ile Asn Asn Ile Leu Ser Lys Ser Asp Asp Gly Asp Ser Glu3665 3670 3675Glu Ser Phe Ser Ile Ser Ile Gln Ser Gly Phe Glu Ala Met Ser3680 3685 3690Gln Glu Leu Cys Ile Val Met Cys Leu Lys Asp Leu Thr Ser Ile3695 3700 3705Val Asp Ile Lys Thr Ser Ser Arg Pro Ala Met Ile Gly Ser Leu3710 3715 3720Thr Asp Gly Ser Thr Glu Thr Phe Trp Glu Ser Gly Asp Glu Asp3725 3730 3735Lys Asn Lys Thr Lys Asn Ile Thr Ile Asn Cys Val Lys Gly Ile3740 3745 3750Asn Ala Arg Tyr Val Ser Val His Val Asp Asn Ser Arg Asp Leu3755 3760 3765
Gly Asn Lys Val Thr Ser Met Thr Phe Leu Thr Gly Lys Ala Val3770 3775 3780Glu Asp Leu Cys Arg Ile Lys Gln Val Asp Leu Asp Ser Arg His3785 3790 3795Ile Gly Trp Val Thr Ser Glu Leu Pro Gly Gly Asp Asn His Ile3800 3805 3810Ile Lys Ile Glu Leu Lys Gly Pro Glu Asn Thr Leu Arg Val Arg3815 3820 3825Gln Val Lys Val Leu Gly Trp Lys Asp Gly Glu Ser Thr Lys Ile3830 3835 3840Ala Gly Gln Ile Ser Ala Ser Val Ala Gln Gln Arg Asn Cys Glu3845 3850 3855Ala Glu Thr Leu Arg Val Phe Arg Leu Ile Thr Ser Gln Val Phe3860 3865 3870Gly Lys Leu Ile Ser Gly Asp Ala Glu Pro Thr Pro Glu Gln Glu3875 3880 3885Glu Lys Ala Leu Leu Ser Ser Pro Glu Gly Glu Glu Lys Val Tyr3890 3895 3900Asn Ala Thr Ser Asp Ala Asp Leu Lys Glu His Met Val Gly Ile3905 3910 3915Ile Phe Ser Arg Ser Lys Leu Thr Asn Leu Gln Lys Gln Val Cys3920 3925 3930Ala His Ile Val Gln Ala Ile Arg Met Glu Ala Thr Arg Val Arg3935 3940 3945Glu Glu Trp Glu His Ala Ile Ser Ser Lys Glu Asn Ala Asn Ser3950 3955 3960Gln Pro Asn Asp Glu Asp Ala Ser Ser Asp Ala Tyr Cys Phe Glu3965 3970 3975
Leu Leu Ser Met Val Leu Ala Leu Ser Gly Ser Asn Val Gly Arg3980 3985 3990Gln Tyr Leu Ala Gln Gln Leu Thr Leu Leu Gln Asp Leu Phe Ser3995 4000 4005Leu Leu His Thr Ala Ser Pro Arg Val Gln Arg Gln Val Thr Ser4010 4015 4020Leu Leu Arg Arg Val Leu Pro Glu Val Thr Pro Ser Arg Leu Ala4025 4030 4035Ser Ile Ile Gly Val Lys Ser Leu Pro Pro Ala Asp Ile Ser Asp4040 4045 4050Ile Ile His Ser Thr Glu Lys Gly Asp Trp Asn Lys Leu Gly Ile4055 4060 4065Leu Asp Met Phe Leu Gly Cys Ile Ala Lys Ala Leu Thr Val Gln4070 4075 4080Leu Lys Ala Lys Gly Thr Thr Ile Thr Gly Thr Ala Gly Thr Thr4085 4090 4095Val Gly Lys Gly Val Thr Thr Val Thr Leu Pro Met Ile Phe Asn4100 4105 4110Ser Ser Tyr Leu Arg Arg Gly Glu Ser His Trp Trp Met Lys Gly4115 4120 4125Ser Thr Pro Thr Gln Ile Ser Glu Ile Ile Ile Lys Leu Ile Lys4130 4135 4140Asp Met Ala Ala Gly His Leu Ser Glu Ala Trp Ser Arg Val Thr4145 4150 4155Lys Asn Ala Ile Ala Glu Thr Ile Ile Ala Leu Thr Lys Met Glu4160 4165 4170Glu Glu Phe Arg Ser Pro Val Arg Cys Ile Ala Thr Thr Arg Leu4175 4180 4185
Trp Leu Ala Leu Ala Ser Leu Cys Val Leu Asp Gln Asp His Val4190 4195 4200Asp Arg Leu Ser Ser Gly Arg Trp Met Gly Lys Asp Gly Gln Gln4205 4210 4215Lys Gln Met Pro Met Cys Asp Asn His Asp Asp Gly Glu Thr Ala4220 4225 4230Ala Ile Ile Leu Cys Asn Val Cys Gly Asn Leu Cys Thr Asp Cys4235 4240 4245Asp Arg Phe Leu His Leu His Arg Arg Thr Lys Thr His Gln Arg4250 4255 4260Gln Val Phe Lys Glu Glu Glu Glu Ala Ile Lys Val Asp Leu His4265 4270 4275Glu Gly Cys Gly Arg Thr Lys Leu Phe Trp Leu Met Ala Leu Ala4280 4285 4290Asp Ser Lys Thr Met Lys Ala Met Val Glu Phe Arg Glu His Thr4295 4300 4305Gly Lys Pro Thr Thr Ser Ser Ser Glu Ala Cys Arg Phe Cys Gly4310 4315 4320Ser Arg Ser Gly Thr Glu Leu Ser Ala Val Gly Ser Val Cys Ser4325 4330 4335Asp Ala Asp Cys Gln Glu Tyr Ala Lys Ile Ala Cys Ser Lys Thr4340 4345 4350His Pro Cys Gly His Pro Cys Gly Gly Val Lys Asn Glu Glu His4355 4360 4365Cys Leu Pro Cys Leu His Gly Cys Asp Lys Ser Ala Thr Ser Leu4370 4375 4380Lys Gln Asp Ala Asp Asp Met Cys Met Ile Cys Phe Thr Glu Ala4385 4390 4395
Leu Ser Ala Ala Pro Ala Ile Gln Leu Asp Cys Ser His Ile Phe4400 4405 4410His Leu Gln Cys Cys Arg Arg Val Leu Glu Asn Arg Trp Leu Gly4415 4420 4425Pro Arg Ile Thr Phe Gly Phe Ile Ser Cys Pro Ile Cys Lys Asn4430 4435 4440Lys Ile Asn His Ile Val Leu Lys Asp Leu Leu Asp Pro Ile Lys4445 4450 4455Glu Leu Tyr Glu Asp Val Arg Arg Lys Ala Leu Met Arg Leu Glu4460 4465 4470Tyr Glu Gly Leu His Lys Ser Glu Ala Ile Thr Thr Pro Gly Val4475 4480 4485Arg Phe Tyr Asn Asp Pro Ala Gly Tyr Ala Met Asn Arg Tyr Ala4490 4495 4500Tyr Tyr Val Cys Tyr Lys Cys Arg Lys Ala Tyr Phe Gly Gly Glu4505 4510 4515Ala Arg Cys Asp Ala Glu Ala Gly Arg Gly Asp Asp Tyr Asp Pro4520 4525 4530Arg Glu Leu Ile Cys Gly Ala Cys Ser Asp Val Ser Arg Ala Gln4535 4540 4545Met Cys Pro Lys His Gly Thr Asp Phe Leu Glu Tyr Lys Cys Arg4550 4555 4560Tyr Cys Cys Ser Val Ala Val Phe Phe Cys Phe Gly Thr Thr His4565 4570 4575Phe Cys Asn Ala Cys His Asp Asp Phe Gln Arg Met Thr Ser Ile4580 4585 4590Pro Lys Glu Glu Leu Pro His Cys Pro Ala Gly Pro Lys Gly Lys4595 4600 4605
Gln Leu Glu Gly Thr Glu Cys Pro Leu His Val Val His Pro Pro4610 4615 4620Thr Gly Glu Glu Phe Ala Leu Gly Cys Gly Val Cys Arg Asn Ala4625 4630 4635His Thr Phe4640<210>3<211>290040<212>DNA<213>人<400>3cttcagcttg gagtactaaa tatattctat gaaatatact ttattttaaa gtgcaatata 60tttgtcaaga atgcactatc ttattaccct taaaaagaga aggtcactga agagtaaagc120atgtcttcac tcttcaagcc taagcttttt cttttttttt tcccaaattg ttctcagcta180ccaattttgt ttttaaattc cccaatacat tgttgtacaa atccaggaaa caaacgcaga240ctttaccaca gcattttcag cagtctcgat gtacctaata atatacatac ttcctgtgtt300tgtagtcttg tagctttagt acaatattaa ccctttaaat gaaacaacac taatctgtcc360tcttatatac acgttatggt tcctgccctg ttatttacta catcagcacg ggtagcttcc420tttaaaaaca aaatcgttaa agatgagagt aggaaaaatg taaatgtgat aagagttcaa480aaaactgtgg tccaggtttg caatgttaag tggaaaagaa cgactaaatc tgtgatcaca540aggggaaaga tgaagccata gtcttattac atgggcacag gacagagaga tctcttttgc600gaaatgagca taccaaccac aaagaataaa acatactacg cccgtaataa aatgggcaca660ggcaggattt gccgtagttc aatgctattc tttgagggcg aatgtgtcgt gggtgaactg720ctgtctagcc cattccgaca cccaagccgc ccaggcggcc cccacgcccc ccggctgcag780ccctccctgt acacacaaat gcacacacgc ccagcccggc cgctggagga actaagatgg840cagcgcagaa agcacggggg ccgagctcgg tggggtgggg aaagaaaagc aaagccctct900gtgttttttc attctgtata ggtccccctc tttccccaaa cacaagggct gcgtaaggca960ttcggtagta gagctcgcag gagcggcggg aggggacgct tgctttgcat actgccagaa 1020acccacgtct ccgatctgca gcgacggttc aggcgaccct cgcgacttgt gaccacactc 1080tccttcacgt ccccttgaca gtagaagtaa gggtgactcc aaatgccttt ttctttctgg 1140
catgcgcccc actcccccgc catcccaggg gagccttcca agttgcccag ctgccaaggc1200ggtagctgtg gcagttgcag ggaccgggga gcgcgccgtg cggaggcagg aagaggagga1260ggaggagaaa aaacgctgca ctggctatca attttgagga gagcgaccgc tgccgctgcc1320gtggaaggaa acgctgccaa ccgcaacaga aatgccactc tcggatacct aagtgagcat1380gtgtgcgact ctgcgcacgc ctagttgcgc agctcaatga catcgggctt cttagcaagt1440tatccagttt ccgggttttg agtgtaccca tcaggctccg taggggcctg tctgggactg1500aacagcagta aagattgaag cgaaattaat gaatggtggg aagagcccgg agaccagggc1560agccacgcgg gtcaatgtcc actagaaact ctaaatgggc agtgactgca cattccacca1620cctgagcctg cgctgtaaat gttttctgtg caagcaacat tcattcattc agccaacatc1680tatttccctc ctgttcaggg ctagaccctg tgctaggagc ctgtgatggt tgaacagaca1740ccattgctac cctacggaaa acctagtgtt ttgtttagta tgtgctacca tttgtgaaaa1800ttcatgtgcc aagttggaca tcctagatcc tttaagtcct ccctcaatct catgtagtgg1860gtattctttt gagcattatt cagaggagat aatttgccca aagccagaca agtattaagt1920agcagagtca ggatccaatt ccagggctgt ctgatttcag agccttttag ctaccctgct1980aggtcttgca atagtaatgg gccctctcat tgcttacctg tcaggcctta gaattcttag2040aatttagctg cctctacacc attgtcttgg ttctcactcc agggttaggg gctgcctggt2100caacactgag gtctttaact gtgctgaagc attaggatag aaatttagtc taataaatac2160ttactggata cctactacat gcaaagtcct gaagtggctg aaggagacac aaaagcagca2220agctctgaag gaacttaggt aaggaaacaa gacaaataca aaaacttaac agggctaatt2280acagtactaa aatactatga ttccacatgt cttgtccttt gttcctatgt ttatcccact2340gctaattatc tttaaaaaaa ctaaatgaaa tccatccatt atttaaagct tcagaaaaaa2400atgcattgct ttcagcaacc ttccacccta aaactagaat taatttactt tcctattcct2460tgttgtttgc acttctcaaa cactaaggag gattttttaa catttatttt attttttttt2520aagagaaggc catccgaaga agaggcacag atataagaac tagtacatag atgaaagata2580cagtactggg atgacagaac ttataaatta ttttgtcagg actgtaaatt aggaaactct2640tcaaattgag agtcaaagga taaaacgtag taggaatcaa agtgcattgg cacgtatatt2700tctagagcat atacttagat ggggttcgta ggttagcttg gaacaaagga ctgagcaaat2760gttacttttt tatagacaac aacaagatat gtgatggatc tgacattttc agacgctata2820
cagtctccgt cacaattatt gtttcagatc gcattcaaga attatcttta aaaatgtaag2880tttgggcaga aaaaaataaa ccattaaatg accactgcca agagataaaa gtgcactctt2940aggtgagtga ggagtcaata aaaaagagta atttaaaaaa tattattgct ccgttgagaa3000caagaatttg ggaatgactc tggcttgtga atactagcct atctgttata ctttcacaca3060caaaatactt tcacacacaa aaaagaatgt gctagattac tacctgtctt tgtaagtaaa3120agctgtgtta aacaagcgta aatcctaatg acaaaaacca tataaaataa gatcgctaaa3180ggcacttcta attgggatgg ttttctctgc cttaaactct gaattaacca gcacaattca3240cagtaaatct tcctgttggc agcagagcct tggagaagag gtagcagaag agcgtgccac3300tcctcctctg gcgatgggca cgttccccct tgctttctgg tcctcgttct tccacctgga3360gggaacctga agtcgaacgt cagtagctga cagcctcccc agcactttcc ttactcttct3420tcaagctgcc agtaaaaccc ggagaagtgg actacttcag attccgcaca acccaacagc3480cccaaaccca aagccccgag gcggagggag tggtggcgga gagggggtgg ggaggaaaag3540gggcggcagt tactgagcat gtgcgaggag tggcgcatgc tctgtgaggc cggcagcttc3600ccattgcggg tagccccggc ggtggtggcg gtggtagcgg tggtggcggc ggcagtggcg3660gcaccgcctc ctcctcacat tcccggggtg gcggggttag atgagcggcc ccagtagcgg3720cgagggcggc gcggggggga ggaggagaag aaggaggagg agaaggaggt cgctgtcttt3780gtagtctccc tgctgcggga gccagaggcc gccgccggag ccgtcgtcgt tggaaaaggg3840ctgtgtgtgc gcgcgcgtgt ctgcccgccc ggcccgcggg gacgaggcgg cggcggcggc3900ggcggcggcg aggatgatga tgtgcgcagc gactgcctcc cccgccgccg cctcctcggg3960gctcggcggg gacggattct acccagccgc caccttctct tcctccccgg cgccgggggc4020gctgttcatg ccggttcccg acggctccgt ggctgctgcg gggctggggc tggggctacc4080cgccgcggac tcccggggtc actaccagct gctgctgtca ggccgggccc tggccgaccg4140ctaccggagg atttataccg ctgcgctcaa tgacagggac caggggggcg gcagcgctgg4200acacccagcc tccaggtgcg tccccagggt gcccttcctt gcgccccatg ccgcccttcc4260ttgcgcccca tgccgcgcgt gcacccgcgt gtgtgtgtgc ttgcgtgtgt gtacctgcgc4320atttattgag ctttcagtcc gctctggatg tctgttggca ggagcactta tcgagatagg4380agggtgcggt ggctgcagtt tctcgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt4440gtgtgtgtct gcctgcctat gtggggggat agtgaggggt ggtaggggaa cctgtgatgc4500
tggaaaaggc gatggtgctg gtcggctgtg acacgtgtgt gtatcgttga ggtaggaggt4560ggcatgggta gagagggtaa gaggatgata cacgaagaga attgcaacag tggcaaggga4620agtggcgttt tcagtgtctc tgccgcaatg ttatttgcac gtgccgcctc tcccatgaca4680ttccttccag ccctttccac ctcccttcaa tctgcctttc accaagagga gtcatggtat4740agattggttg agttttggat aagcaggccc gagggatagg cgtgaggttt gctttaaaaa4800gatttatgaa taagggacgc agatggagat ctgtgtggat gcgaaaggtg tctgatagag4860atttttagag ctgtactttg ataaccgggc cttttggtct tctagaaatg aggttgcagt4920tactcttgtg atctgatgtc ccatcggcca tttctcttca tcctctgttc gatttcctct4980gttacttaca ggctttagag tagagagggt tttgtaggga aaaggggaaa actgcagaac5040ttcagaatat gacagctggt acagcgttca actgccacta gctagaaaat ctgaatagcg5100cgtagatttg tctcttttac ttgggttggc tacaccttca gcttccttga tgtagatgtt5160cagttggttt cattttcttt ttcttaaaat gtgggtgctt ttttttgctg agggctctcc5220tattgttttc ttttccatgc ccttacatgc cgcataccct gaaggttggt gactgacttg5280tttcaccagc tttgacagtg gtaattggga gttggcttca tctttttttt cccacagctt5340cttgcagtac atggggaaaa catagtagga aactgtcaaa atgagttaaa gagtttactg5400ttatttattg aatgtgcaat ttattaggca attgttaggt gtctttttct cccattatga5460aagggacgag gctgtaagtc aggtatttgt agcttatcac taggtatcat ggcttatctg5520ttggagacag ttgtaaagct ggagctttgt tcacactttt tctaggttaa ccagttttct5580catgtctgtg attgtttaca ggattgggca cagcagtagt tttaccatat ggatgtaaac5640gtgtgtaact attttttgct atctgaactg aaggccagaa tggcacaagt ctgtttcttg5700ctaatgcttt tgaaaatagc acaaaactac gtgagacagt ttgccctgat aattaggtat5760aattttgatc tgtaaatctc taaattatgg caccgcagga acatctttac aaactcctct5820caaagaattt aaatttattt tgtgtatggt ttccagactt atgcaataat ttttgacttg5880tatattcctg ttttgacttc ctactactag tgtaatatga tttctgtgta tctagaacta5940tattaacggt tttagacaaa attaacatag attaatcata taacatttgt gaatgatttt6000ttagcccttg gtccattttt gtacaagccg tcaatgaaaa gtaatgaaga attagttcaa6060gtatttcaag aactactcat ggaaatcagc attatgttat gatattgcct gctaatgctt6120gttcaacgca acattattgt ttatttaagt tcacattatt ttattacaaa aagtagcctg6180
caactactct attgatgatt tgattaagaa ttaaatcaca tttcttatta aagatgttga6240ggaaagtaga cttggggttt cggctctgaa tgaatgatag aattatttgt atagggacac6300aagttaaagg agaagatgat atcttttaga gagactgaat ggagcaataa tagagaatga6360agctctagtg agcatcaggt ttaaaggtgt gagagaaagg agacagtgaa ggaaacggaa6420tcaaaattaa gaagaaaatc atgaaacagt agcctctgtg gctctaggag agtggattga6480aaattctcag aagtggtaaa tgttgaagaa agtgagaacc gttttcaggt cattgataac6540ttttgaggaa gtggtcagaa tagagggatg tggtgaggag tgaatggaat gtgtttttgg6600taactcaagg ggatttcaaa gttgagggaa ggatattcaa gtataggagg ttgggtggga6660tatgcaaata tgggaggcta aatggagaga cagagtgaag aagtgagaga ggggataatt6720gataggaaaa ggtcaaaggg atctcaggga caggaagagg tcaaagggat ctcagggata6780gagttaaggt gacaggtgga gaggttagcc attgaaaaaa ggagcagtta atatagaagg6840ggtggaggtg agtacttgta aatataaaga ctgggagttt attgaggata ggcatcatat6900cctattcgtt ttgataatcc caatgtctag cacatttttt ggcatgaatt ctgtaaacat6960caagtgcctg ctctgtgcca ggcagtattg cagatgctga agatgtagcg gtgagcaaga7020tagacaaagt ctctgccgtc ttggaaaatc cactccaatg ggagaaatag acaactcata7080atttcactta gtaataagta actgtgaagg caataaaacc tatttcatgt gatagggcaa7140tttgtagtgg ggctatttta ggtaacatgg tcaggaaatg tctctttgag agttggcatt7200taaattgaat tgccatgatg aggaggcaga tgtagaggaa gaatgttctg ggaatttata7260aaggccttga aatgggaaca ggcttgtgtt caagaaacaa aaacatgatg agtgagttat7320cagtcaatgt caaatgaatt aattactctt tgctttaggt gtacagttga gaagttgaga7380gtttcaaacc taatggactc agtgactttg atgacagtct aacaaagtgg ttaagagctt7440gggcacagaa gctagactgg ttttgcatcc tggttttgtc atttgctagc tgtgtaactt7500tgagtatatt acataacttc tatgcctcag ttttttaaat atgtagaatg gggatcataa7560tactaacatg aattgttttg tagattaaat gaatcaatgt atgtgcttgg catgttgact7620gctatgaaat attagttact gttttttgtc tgtttgtttt tgctgagaat gaggggtgtg7680gggacttgaa gagaaggcaa ggatcatgtc ttactctttt atttttggtc tcagtgactg7740tgtagaatgt gggaataact ctttcattta tccagtgact gaatagatat atttttggat7800gaatgaggcc aatcaaaatc ggaacattct gttggggaaa ggggttggac aggaaatgta7860
tgaaggaatg tatgatctgt tctgaatgtg aatggtggta taaccatgca cttgggaggc7920accatagcac agtggctaaa tgttagagca ctggaggtag actcccttag ttcacgtcta7980cctttttcta cttgtgttaa attgggcaag ttaattagac tttgtctgtt tctttacttg8040taactttgta gtgttatcaa aaggacatta tgtccgcacc tggtaagtgc ccatgaaatg8100ttacctgttg ttattattaa aatcacattt aaaattccaa ccagcatggc actgtatgtt8160tttccacaca aaatagaaat ggagaaaacc tagttgagtt gatttagggt taaagaaaaa8220agaaatggaa ggttttgggg acagtgtttg actatatggt aaaaaggaaa gtgatgccaa8280gagggaactg atagactgga agataaagag gagtcagagt atctgggtcc tgaagtccta8340gaagaacaat gttagtgaaa acaatagagg ggattgtgac caaaatgggg aatctaaact8400taaccccctt agttttttca gctgctttat ctctttcttg ttagaaatgg tatttgagtc8460catccttcag atgcatggga ttttggttgt aagaggttaa gtagtcattt tagcaaaagt8520tcagggatta ttttatttta cttattttga ggcaggctgg agtgcaatgg tgtgaacata8580gctcactgca gcctcaacct cttgggctca agtgatcttc ctgcctcagt ctcctccagt8640agctgggact acaggcatgc accaccatgc ctggcttgtt ttttattttt tgtagacaca8700gggtctcacc atgttgtcca ggctggtctt gaactcctga gcttaagtga tcctcccacc8760tccacctccc aaagtgctgg gattatgggt gtgagccacc atgctcagct tgctcaggga8820ttattacttg gcactaacca ttggccctat aaaaatttat aagacacaaa ttctagactt8880gagtttactg tctatctgga agtgcaagat acaaaggcag atacttatag aagtgttaaa8940tgaaatgacc aagaaagtat aacctactat gacaaaggca tggcattcta ggctggctta9000aaagcatcag cagagacagg aaagcacagt ttgtatgtag gtaacagtag gaattctgtt9060agagctagaa tgcaggtgtc atgaaggaca gtagtggaat tcattctgga aatggaggct9120ttggctagga tggggcatct tgaagaccaa ggtaagaatt agattttatt cagtagacac9180tgatcaccct tgatggtttt aagctatgaa gagacgttat ttattgtagg aatatgtcag9240gaagtaatgc gggattgaat ctgtatgtgt acatctgtat ttgtgcatag gtagtaattt9300tcttcatgta gctgttatat cagtggaatc ctgtatagtg tataaacaca agttaaggca9360aaaactgctc tattggtata ctggtaggta gaaatgtgaa atgaattagt ttcctaactc9420aagagaagaa aaaaatcttg cctctgggct taaaaggaaa ataaaaatgt caatctaaaa9480atacttttgt cctttaatat atttgtttaa aaaatcaaca gaatagtctc acttttttct9540
caaaaaccag attaaacatt taggttttct gaggaggggt taggttaatt gaggtttaat 9600ttatatacaa taaaattcat cctctttaaa tgttcatttg aagagtcctg aaactatcac 9660cacagtcaag tatagactgt ttccatcact tcatataaaa agttctgtca ggccctccta 9720cccctggtta ctgaccgctg caaatcagtt ttctgtcact aatattttac cttttctaga 9780atgtcatata aatctagctc cttaagggca gggcccccag gtattgttca tctttctgtc 9840ttgtatctgt atttcctgta agtggaagct agaccttaag ggtttattag gtgttcatta 9900gaaaattttt tagcaagaat attgtatatg gcgctgggta ctgcatgttg catcccatcc 9960agaggttgtg aggttgtctc actatttaat atgttaaatc tgattacttg ggtaagacgg 10020tacctactag atttcatctc tgtaaatgta tgctttttcc atttgtaaag aacaagtaat 10080cacagggtaa aaatttgagc acattgtgaa tgccttttac ctaatgcttt tagcattctt 10140tgataatcct tgtctgagtc aataatttca ttaggggttg caaaatgatg aattaaaaaa 10200taattctgtt atttcttcta tgtttattag ctggtactct tctgcaaaga agagttggcc 10260ttcactgact gtcagtgaaa tgtagtttct cctaatccag cagcataaat gcttaattct 10320cttccctcat tagccaaatt ggtgtaatag tccagtggtg gtgtggcagg tgctgtggtt 10380aggctttcag atcccgcttt caggcacatg ttcccctagc tcttggcaat attggcagct 10440aaaactgatt gcagctgagt cccactccac gcattgccct gagccaagag ttgcctcccc 10500cagagtcatg cctcctcttc aggggcagtt tacatctaat atctatacag tgtagggata 10560taaatgccta gcgcttttgc tataagacaa gccaaccttg aagtctatcc caacaccaga 10620gagctctccc atgggtctgg ctgagggctt cctgggactg cattgcagat caactccttc 10680ctctgttcag tcttacgtct ttcactgccc cacagttgtt ggttcggagg gcactccgca 10740ataaacttcc ggtgtgcaca tctgcatttg agagtctgtt tcctgggaac cctcctaccc 10800aaagcagttg gtgccaggag tggtctcaga aagtagactt tacagtgagg ttttgaagct 10860ggattattgg ctggctggct ggcaatgaga atcgattctg gtgctagatg gagcactaat 10920actgataacg cttggcatgc actgtagcta tgcaacttaa aactttcact ggtggtgagt 10980tggaatgcta ttcctgttga agggaatgca ctggtgagtg cactaggctt ttgagaagtt 11040cagagaaatt aattatgagg acaatcatca gatggctttt ggtaaataac attgatagat 11100tggagaaaga tgatgcaagg tggaagataa ttgggggcaa aatgtaaaag gaaatttatt 11160tagcatcatg taaagaaaca catatacagt aagaggaaaa atctaaggct tcaacccaaa 11220
acttaatttt aagggtagca gagcaccaag gtaggtagaa ttctctctca gctatgctaa 11280ggtcagtgtt ctggttgggc aggaatcctg acccttagga tggggcatct gagtgcactt 11340gaaaaccctg aaaccccaaa ttcctcttaa ccctctgggc ctgcagaagt ggccttcttc 11400tactagctag aggggttgct tgcagactgt gctgtccctc ttacccccag ctcctcctct 11460ctcgcctact gactatcacc aaaccaacaa ctagggttaa gtcacaagaa cacctagttc 11520ggggtgctgg atctgcgaag ggaggaaggg actgtacaca caaacacaca cacacacacg 11580ttcccaaact gcaccaaggc accccaaagc accaccacag acacaaaaga ataccatggg 11640atatttcaga attttaaagg aaacacagca atatctgtca caaattacta gcttgaggta 11700gttcacggtt ttgacattag ctcatgccac atttcttttg atgatgtcat atttttgcag 11760ggttggattt ttactagttg tgatacagat caagcactgt gcaaaaatca gtgtggaaca 11820ggaaaggagg gtggcatgtt cagtctgatt ccaagtttga gaagttgttc tgtgtctaac 11880aggcacacat gtcatattat caagtaattg aagtttttgt ttgtttgttt gttttgaggc 11940agagtctcgc tctgtcaccc aggccagagt gcagtggtgc gatctcggct cactgcaacc 12000tccgcctgcc aggttcaagt gattcttctg cctcagcctc ctgagtagtt gggattacag 12060gcgtgtccca ccacactcgg ctgatttttg tatttttact agaggtgggg tttcaccata 12120ttggtcaggc tggtctcgaa ctcctcacct cgtgatccgc ctgcctcgtt ctcccaaagt 12180gctgggatta caggcgtgag ccactgcgcc tggcccagta tttgtagttt ttaagaagga 12240aataaaaata tttttcttcc aatttatgtg ttattttttc aaatgtctac tcatttgtta 12300ggacatgaat tcttgttatt tggatctaac tacttaataa acactactgt ttggtatttc 12360ttttggccta tgagtgcatg gaaaaattac cgatgatggc tgtgcatggg cccaataaaa 12420gggttttcat tcaccagtgc tgatctagca actgctgctg ctgaatatcc agcctgacag 12480cagcagagat caatcatcct tcaagcaggc caaccagtcc ttatacagtg gaaaggacca 12540tgattcattt tgattggatt tgagacatat tctgggtctg aatttgtctt tccagcaggg 12600tctcacccag gactactgtt tgagggcttg caggatgttt gatcctctac tacagggatc 12660atttcagaat aagggaccca ctgttatgtt atgttatgtt atgttatgtt atgttatgtt 12720atgttatgtt atgttatgtt atgttatgtt ttgagacgga gtcttgctct gttgcccaag 12780ctggagtgca atggcgtgat ctcagctgac tgcaacctcc gcctcccggg ttcaagcagt 12840tctcctacct cagcctcctg agttgctggg actacaggtg cacatcacca cgcccagcta 12900
atttttgtgt ttttagtaga gacggggttt caccatgtgg accaggatgg tctcgaactc 12960ctgacctcgt gatccggcca cgtcagcctc ccaaagtgct gggattacag gcgtgagcca 13020ccgcgcccgg ccgggaccca cttttataga ataggagaat catttgtccc atcttgcacc 13080acaccgtcca gaagctgatg acttgataga gcagtaaaac agtcctttga tggcacagct 13140gatgatgaga tcctgcagct tgggcatgat gccctgcaag ggtgaggcgc ccattctcca 13200gtgttcccag taggaagatc acatgaaatt aggagtggct ccgcatagca tcactctgtg 13260aatcacctgg gcagtttctg cttcccatct ccatagctct gatggcaagg ggttaaaaga 13320tcctggttct cagaagagaa acatttccaa cagagggcgc tatgagaatc ctgttatact 13380ttaaagtaca gctgccacta ggatttgggg ctccttttct aaataaagaa gtcaccaggc 13440tgggcacgat ggctcacgcc tgtaatccca gcactttggg aggccgagat gagtggatca 13500cgtgaagtca ggagttcgaa accagctcag ccaacatggt gagaccctgt ttgtactaca 13560aatacaaaac aattagccgg gcatgttggc gcgtgcttgt aatcccagct aattgggagg 13620ctgagtcagg agaactgctt gaacctggga tgtggaggtt gcagtgagcc gagattgcgc 13680cactgcactc cagcctgggc aatagagcaa gactccatct caaaacaaaa aaaaaacaac 13740aaaaaaagaa gtcaccattt ggacagggtt aatgtccttg ttcatgtgga ggaggtaggg 13800ctgtgttcca cagtggagca tggaggtttg gcacacaggt gatctataag gtgtctcttg 13860gtacttcctt gtccagtgtt tgtggtaaat ggacacgaga aaggcccagt gaccacccca 13920tgaagtcagc cacctggacc aacagaaata ctagccatag attaaaggaa tctagaatgg 13980ctagtagaga agagttgttg atatcaattt taccgtctgt agtggctggg gctgtggttt 14040gacccacttt ccttcctctg gaaagtttct ccaggaaatg acactcacca gaattctgga 14100ggaacttttt ccatgcctta tttgaagcaa gtgaatccta attgtataat aggcgaaata 14160tagtgaatcc tgtattgtgg aacccagagc ccctgcccag cactgatgtg ctcaaaattc 14220ccccacgact gctgggaaca ttggttggct acagactctt ccctagttcc aatatgggca 14280ttgcctttgg caataggtga tggccctagg tgatggatca ttcttggggg cagcctgaat 14340gaactggttg atcggggcat tttatggttt ttagtgctac tgtaactagt ttagtttttc 14400ctatttttca gttgtttgat atgaatatat aacaatacag ttgcttttga atgttaacct 14460tgtatctctg atcttgttaa gctcatttat taactttaat agttttgtaa attccctggg 14520atgttctgtg taaataatca tattgtcttt ctttctgatc tctagattct cctacccctc 14580
agcccctact ttattccaat tacttaattc tcccttgttt ttaatctgag agaaaaacaa 14640ctacaaatta atcttacagt tttttaaatc attaagtatg atattagctg taggtgtttc 14700aatggcctct atcaatttga agatattccc tagtattcct actttgctga tagtttttat 14760tttttatatt ttaatttttt tttttttttt ttttgagaca gagccttgct ctgttgccca 14820ggctggagtg cagtggcata atctcggctc actgaagcct ctgcctcccg ggatccagca 14880agtctcctgc ctcagcttcc tgagtagctg ggattacagg cacacgccac tatgcctgac 14940taatttttgt atttttagta gagatgagat ttcaccacgt tggccactct ggtcttgaac 15000tgctgacctc aggtgatcca cctgcctcag actctgaaag tgctgggatt acaggcgtga 15060gccatcgcac ccggcttgct gatagttttt aattataaat gtgtattgag ttttgtcaaa 15120tgcttttcct ctacctgtta aaatgatcat atagtaagag aacaaaagga aaattgaatt 15180acattaatta atttttgaat gttaaattaa ctttgcattc ctgggataaa accatcatat 15240ttggaatgta ttgctgaatt tgatttgctg gtattatgct aagaattttc aggtttatat 15300tcatgaggga tattggtctg taatcggttt tttttctttt cttttcttgc taatcctgtc 15360aggctttaga attagttatc ctcgtctcat aaagtgagtt acatagtaat cctaccctac 15420ccagtcctac ccaattttta aaatgtttgc gtaagattgt tgttatttct tcttgaatgt 15480ttgacagaat tcaccagtgg caccatctgg tcctggaatt ttattttggg gaagcttttg 15540acaaattcac gttctttaat agttagagcg ttattcagat tttctgttat tattcatgtc 15600aattttggta acttgtattt ttttggagaa gttcattcat ttcatacttc taagttgttg 15660aagtttttag catgaagtta tccatagcat tctttcatta tcctttaagg tctatgggat 15720ctctgataat aactccctcc ttttttaaaa aaaatatttt tttaagagca gttttaagtt 15780catagcaaaa ttgagaagag ggtacagaga ttttccatct atccctttcc cccactcacg 15840catagcctca tccatatcaa catcccccac cagagtggca tttttgtggc cattgtttaa 15900cctccactga cacatcataa tcactctcaa gtccagagtt tacattaggg ttcactcttg 15960gggttataca ttttttggtt cagaaaaatg tataatgaca tgtatttatc actgtagtat 16020catacagaat attttcactg ccctcaatat catctgtgct ccacctattc ctgtttctct 16080gccactcaac cccaggtaat cacttactgt ctccatagtt ttgccttttc cagaatgtgc 16140tatagttgaa atcatacagt atgtggcctt ttcagattgg tttccttcac ttagtaatgt 16200gcatttaggt tcattctttt tttgtgtatg agacttgata gctcatttct taccactgaa 16260
taatccacca tgtagatgta ccagaatttg tctgttcacc tacaatttgg tgagtttgga 16320ggacaattgg gttgcttcca agttttggca attatggata aagctgctct aaacatctgt 16380gtgcaggttt ttgtgtggac atacattttc ttttttagaa attgacaaat gacattgtat 16440gtttttataa tgtacagcat gatgttttga agtacatata cattggggaa tggttaaatc 16500tagtggacat gttttcagct cctttgggtt aataccagaa acagtgattg tgggatcacg 16560tgctaagagt aggtttagtt tttgtaggaa atcaccaaac tgtcttacaa agtggctgtt 16620tcatttttca tttccaccag caataaatga gagttcctct tgcattaaag cctcgccaac 16680atttgatgtt gtctatgttc tggattttgg ccattctgat aggtgtgtag tagaatctcg 16740tttttttttt tcatttctct gatgacatat gatgtagaac atcttttcat aacgcttatt 16800tgccatctat atatcttgtt cggtgaggtg tctgttaagg tctttgcccc atctttaatt 16860gagttgtttg ttttcttatt gttagagttt tgagagttct tggtattctg tgagggtgta 16920actgaggaga tggggaaaaa agttcttggt attttggata acagtccttt atcagatgtg 16980tcttttgcaa atattttctt tcaatctgtg ccttgtcttc tcgttctctt ggcattgtct 17040tttgcagagc agaggttttt aattttagta ttcagcttat taattatttc tttaatggat 17100tgtaccttgg tgttgtagct aaaaagttat ctaggttttc tcctgtgtta ttttctaaga 17160gttttatagt tttgcatttt acatataaag tccgttttcc attttgagtt aatttttgtg 17220aagggtgtaa ggtttgtttc tagattcatt tctttgtatg tgatccaaag aaccaacttt 17280tggctttctt aattttctct attattgatc tgttttctat ttcattggct tttccatcat 17340cattgtaatt tatttctttc tactttcttt aagtttactt tgctgttcta atttcttaag 17400gtggaatcct aggccattga ttttaaattt ttcttccaat ataagtattt aaagctatga 17460atttttatct gggctgtgct ttagttacat cccacaaatt ttgactgcta tatatttgtc 17520atttagttca aattattttc caatttattg tttaaaatta ttacaacttt cagatatggt 17580gttttcttac atattaatat attattgtta ttactttcta atataaacct tgtatgattt 17640tgatctttaa catttattga gatttgtttt attgcccagg atatgttctg tcttggtgat 17700tgtattatac acacttgaga agaatgtgtt cggtcatctt tggacatgaa gttctataaa 17760tgtcagttaa agttggatga taagtgttgt ttggatgttt atgtttttat atttaaagtt 17820tctcctctta ggcaactagt atagaacctt gcccttaaaa aaagaaatcc actgtcaatc 17880tttgcctttc aattggagtg tttagattat taatatttaa tatgattaat tattgacata 17940
tttaccatat caacagtgat aggtctgcca ctttattgtc ttctgtttct ctttttttgt 18000tcctttcttc ctccttttct gcttctttgg gattatattt tgggattatt gggagtattt 18060cattttagtt gctctattgg tcttttcagc aatatctctt tgtatttact ttttttatgc 18120cctagggatt acagtatgca tacctaactt tttacaggct acttagggtt attattatac 18180taattcactt aaaacacaga aaccttgcaa ccctacagat ccttttattc tcccctcccc 18240cagagacctt tatgttatag ttgtcatgca tactatactt ggcaaacatc accaaacaat 18300gttatagttt ttgttgtcga cagtcatgtg tactttatag aacttagagg aaaaatctag 18360tattttgtgt tttcctatat atttattgtt tctattgctc tttcttcatt cctgaagatg 18420cagcatttcc tttcagccta atgttgacct tagcttttct agcagatcag tctgctgggg 18480atacattctc tttgtttttc tttgagaatg tatttatttt actttcattc ctaaaggata 18540ttttaggtgg atatataatt ccgagtagat gcttgcttcc ttgagcacct caatgatgcc 18600atttagctgt cttttcactt ctctgatttc tggtgaaaaa tctttgtaat gtaaacctta 18660ttcccctctg tgtggtgtgt aatttttttc tagctgcttt caaaaatttt ttctttgttt 18720ttggttttca gcagcttaat ttgatgtgta tctagtcatt ttctttaagt ttatcctctt 18780tgaagagtac tgagcttctc aaatcggtaa atttttggct ttcactaagt ttagaatgtt 18840ttctaccatt gtgtctttct tctgacctta caaaattcta gtggcaatta tgataaaact 18900tttgagattg tttcacaggc ctctaactgt tcagtctcct atcaattttt tttctctgtt 18960tttcagatga ggttattttt attgatacac gttcaggttc acagactctt ttctcccatc 19020tccattttct tattgagtcc aaccagtgaa ctttttatgt attgtttatt tcacttttaa 19080catttacatt tggttcttta aaaaaaaaac aaaaaaaaaa aaacaaaaaa aaacttctct 19140ttccctgccg agaacctctt tccattccat tcagttgtcc ttgtctttcc tcaatgtagc 19200atatttatag tggttgcctt ccagtcattg ttggataatt acaacatgtg catcgtctta 19260gggttggcat cttttgattg tctcttccct tgaagttgtt cacatttttc ttgtgttttg 19320cattgtgaga atttgggatt gtatcttgga catggttaat gttatgtttt gtaaactcta 19380gggtttatta taattctctg gaggatgttg tgttttttgt tgtttagggg atccctttct 19440gtgtcttact ctttttcagg acttcttacc cattttccat catatcagtt tcttttctca 19500gttcctctgg ccagaaagag ttttaacttg gaattttaac ttttgggttg tagccctgta 19560atgcagtgat ctcttcctgg ccttcaggca aagctgttag tgaaaggaga aaaacaacca 19620
aactgggaaa tttactcttc tgtgaatcac ttttccaagt tttgactccc ctccagaatt 19680tgcttttatt tgtttttcag agtcctgaag tacttttttt gtctttttaa aaattttgcc 19740caaagtagtt gtaaacagtg gggaaaatag gctgttttgg ggtttatgcc aatgtactgg 19800aaccagaact ctcttatctg gctttaaaaa aagattttcc cttttattga tttttagcag 19860cttgactgta ttgtacctaa atatggtgtg tgtgtgtgtg tgcatgcgca cgcacacacg 19920tctgtgtctc tgtgtgtgtg tatcctgctt ggtatccttt cagtttgtta gatctgtgtg 19980atgtcaattt ttatttaact tgcaaatttt ggcctttatt tcctcaaata ttatttcaga 20040ctcaatttct tatttccttc tgggaatcca attatatata tatgatactg tttgatatca 20100tatgtgtcta taatcatata tatgacatat ttatatgacc agatagtaaa taacttagac 20160tttatgggcc atagagtctc tgttgcaacc atgcagctct gttctttagt ttgaaagcaa 20220ccacagacaa tatatataaa tggataagca tggctgtgtt ccaatataac tttatttaca 20280aagatagaca ggaagctgtt tttggcatgt agactatagt ttgctgaccc ctgagtaggt 20340ggtaatcgtt gctggagcta cattgtgaat tcagggaatc aaagaagtgc aaaacagaaa 20400tgttggaaat aactacaatg taggtagcgg ggtgcataaa aatgaggagg taggagatat 20460ggctagaaag atatgttagg actaggttat tatgttccct gtattgcttt atttgaaaat 20520agtatggaac aatcataggg tattttttgt ttgttttctt ttttttgtct gtttttctga 20580gacggagtct cactccgttg ccaggctgga gtgcagtggg gcgatcttgg cttactgcaa 20640cctccgcctc ctgagttcaa gagattcttc tgcctcagcc ttctacatag cagggactac 20700aggcacccac caccacgccc agctaatttt tgtattttta gtagagatgg gatttcacca 20760tcttggccag gatggtcttc atctcttgac ctcgtgattt gcccgcctcg acctcccaaa 20820gtactgagat tacaggcgtg agccactgcg cccagccaat cataggtttt taagcaatgt 20880aatggtagag ttaaactttc aatttataaa gataatctct agtagtgtta tagaggatga 20940atgaaaagga gcagatctgc aaagcagggc tacctttaaa gagacagttg cagtaactct 21000gacaagtgaa aatactgaga aaagataatg ggaatagatg gaataaagtg gatttaatga 21060tatatttagc aggtgactag atctcaagtg caaatataat aatgattttc atctagatag 21120aaagggagga agagctagtt tctcaagagt ccattcttgg gattcttctc tctgaatttt 21180cttctttggt tgtctcaggc ttatgatttc agatatgcat ttgacttcct ggtctgtggt 21240ttttaggtaa aacctctttt cttaagttct agcctgtatt ttgaaaagtc tactgctagg 21300
attctctcag tcctgttcag atacattatg ttcagtatat tcaaagtctc ctcgtgaaca 21360tactgtcact cacaaactgc ctcttttccc ttaggccagt gaaaccgaag tctgagatag 21420gttcttgagg ttggcaataa gaaattataa tgagccaaca cccactcact tcacctcctt 21480ttttcaggag gcagggaaaa gtgggaaagt gaggctggac atgggagtat ttagaaatag 21540tgtttaataa tgtttaaagg gcaagaagga aggcagtagc ttaaggaagg agacaaataa 21600ttaatctttt ggattagata acctgtagga gatgttctta gtgtcctagc catatccctt 21660tggcatccat ttgcatactg tcttctgcaa acatctatga ctatctgcct gaaaagaaac 21720acacttggcc tgtgtgcagg gcaactcaca tgcgccagag agttaatgcc ttcagaaaaa 21780ctttccacca gtgatggatg gggagttggt agataaatac ctttgtctca cctgcattta 21840ggataactga gccatgtttt ctgctgtcta ccagagttct ccactggttc aggccctagt 21900tccccatcag ggtaactggc ttcataatat gtcctttctt gacttccttc ttttccctgt 21960tttacttttg ttttccctac tggtgttttt cctgggatca cctctcaaaa taactactgc 22020acttgaattc ttgaattgac gtctgcttct ggggaaaccc aaaccaaggt agacatttga 22080atttggcatg ggaataataa taaaaatgtg tttagcactt actaaatacc aggcataatt 22140ctaagtgtga catcatccag tgggagaggt actgttttca ccctattgta tggatgagag 22200aactgaggca cagagagatt aaagtaatac caagatcaca gtgctggtaa atgacagagc 22260ttgtatttga acccaagtca tttgtttcta aattctgtac tcttaaccta tcttgacata 22320gcaggctgaa ggaaaagggc aaggatagta aaaaagagag agagaatggg aataattaat 22380agagtaaggc cctgggttag agatcatttc aaggtgggtg gaagaaaagc aaagggagta 22440catttgtaat ggtgtctcta ttttattggt taaatagatg gttagattct ctgctctgag 22500ttgatgggta ggggagggat tttgaagtag ctggaaaggt tctagatttt tatggtagat 22560gtaccttgat agagaactta actggctcta gaccagcagt ctaatgatgt tcagtaactc 22620caatagctat tattgtttag ggttgttata gcttattgaa caaggtttgt aactaaccag 22680tatagaagtg aatatagaac atgagcagat agctcacatt agaagtgata tatgtagtaa 22740agacttaagg gacaggaaga ggaagaaaaa ggagccagta aagaagactg agaaggaacc 22800accatcagag gtagaaggaa cactaaagta gaggttctag aagctggcgg gacagggggc 22860agggatgtaa agaagtaggc actgtttggt agttatcgta tgtaggagaa tgtgaaggat 22920aaagactgaa gatgggcagt tgaattggct actagaaagt ttgtggtatt gagaagtctc 22980
atagacttgc atgagtttga tagtaaggtg ttaaaaaact tcaatacaat gaagtttttt 23040tttttttttt tttttttttt tttgagacag ggtctcactt tgtcatccag gctggggtgc 23100agtggcatga ccatagctca gtgcagcctc taactcctgg accaaagcga tcttcccacc 23160tcagccatct gagtagctgg gactacaggc acatgccacc gtgcctggct aatttttttt 23220tttttaatag agacggggtc ttgctatgtt gcccaggcca ggaccatttt tttcttcaag 23280aaatttagtg atgaagaggg atagaggatt acttaaactt ttttcccctt ttgaattgga 23340gaatgacagt aggcctctaa ggttagaaac cacagaaaaa aagcataaag ggtaaacaaa 23400gagagggatg tatggttgag caaaatctca aggatggtga atgaaatggg atctggtgta 23460tgagaggaag tgcatacttg gatagagtaa gagctgtctc ttttgggatg ggggaaggag 23520gaagtttcag atttgaaaag gtctcaggga catattttta gcctttttat gtttctgtga 23580agaattccag attctttttc tgatctgttt tctagatctt aaattttcta ttcagctgtg 23640tcctgtacac ttaaatccac ctatggagtc tgtagtgtca gttatttttc atgtctagtt 23700tgattttttt tcaaatgtgc ttggttgttc cccattccct gcagatattt taatgatagt 23760cttttatttc tttaaatttg atacacatgt tcttgttgca atctttccga aaatcccacc 23820atctgaagtc tatataggtg tctttctgtt gcctagttct gctgttctca ctcagagtgc 23880cttgtatctc tgtgttctta gttatttgct tgctctcatt cctcttgttg ttctctgagg 23940cctgggatga atagattgta ttcatgatat cattagaaag tgttacagtt ttacaattgc 24000ttcaaggctt gagtttccct ggcctaccta gcctatgttt acttaagagt acaaatatgc 24060atgaggcagg gtctatagcc aaagcttttc agagagttct cttcccctca cttatgctcc 24120cttccatatt cccctaccct cttttctttt tccttctgct gctctgccca ttgccaaggt 24180agctttattt atagtcctcc ccccatgtga agacttccaa ataagatcaa gagactgaag 24240ctatatataa gaaatctgtt ttcatctctc ccctaaatca agttgaactg ttaaaatctc 24300tttcttatta ctaattagcc ggctaggtta agtaaataaa agttttctac ttagtaatga 24360gataactatt cctccctaat ttattcattg atgcttgaac atgataggag tatcattaca 24420ttaattacac agaatgaatc tggcttctat ggaaaagctt cagtttacat aaggtgcttc 24480atagattgat ttttaaaagt tggtcttgta tccagtgacc ttgataattc ccttgttaat 24540tctttatttg tggattattt tgtattttct ataaatataa ttatctacaa ataaaaaatg 24600ctttactttt aatttttaac cattatgact tttatttcat ttgcatcact tactgacctt 24660
caatattgtg ttaacagaag tggtgatagt acaaatcctt gcattttcct cctatgccta 24720agtggaaata cttaattttt caacattcag tatagttgaa tacactgtta aaagtaggtt 24780ttttatagat aatctgtatt agattaagta aaatctgttc ctggttcacc aaggagtttc 24840atcactagtg gttgttgagt tttaaaaata ctttttctgt ttctattgaa gtgatcatat 24900gttttgtttc ttttgttctg ttattatatt gaattatgtt gattatgtat ttattctttc 24960cacatgttgt tagacaaaat tatttgtagg taaatggctc tctctttttt taaaaataaa 25020aggggtaata cacataccat aaattcaccc ttttatacaa cttattggtt tccagtatat 25080tcacaaagtc atacaactaa acatttttgt ctctccataa atcccaaagc tattagcagt 25140cagtttctat tctcctttct atcagttttt ggcaactact acttcctgtt tttatgaatt 25200tgcctaccta gacattacat ataaatggaa ttatacaatt tatgaccttt tgttgccatt 25260gtttctttca cttagcataa tgttttcaag gttcatccat gttgtagcat gagtcagtac 25320ttcattcttt ttttatgggt aaacaacatt ctcttatatg gaagtgctac actttataca 25380ttcattagtt ggtgagcatt tgggttgttg ctattttttg gctgtgcagt atttttgctt 25440ctatgttcat aggagatatt ggtctgaaat ttttgtattt tgaaatgttc tcttcaaatt 25500ttgatatcag agttatgctt gtcttatgag ttgggaaatg tttcttcttt ttatattgtc 25560taaaagagtc taacattgat aatgtttgaa agaactacca gtgaattttt aaagtatttg 25620gaagaattca ccaacaaagc catccgagtc tagagttttc tttgtggata gattttgatt 25680agggatccag ttttttttca atatatagaa gactattcag atattacatt ttttttccct 25740tgtttaatgt tgagaagtta cattttttca aggaatttat ccattttatt tgtcaaattt 25800atgggcagca tctgtagtga tgtctccttt cttatttctc atattggtaa tttgtgcttt 25860tacttttttc ctgaccattt ttgctctagg tttattatat ttatttcatt ttattttaaa 25920atatttgcta tatatttaat tcacaaataa ttgtacttat acatggggta taatatgata 25980ttttgataca tgtttacaat gtgtaatgat caaatcagga taattagcat atttatcacc 26040tccgacattt atcattttat catttctttg tcatgagacc attcaaaatc ctctcttcta 26100gctgtttgaa gttgtataat acattgttgt ttatgatagt caccctgtgg tgctatataa 26160cactcaaact tactcctact ttctagctct gattttgcat ccattagcca acccctggct 26220actcttcaac aactcttcta ctttctactt ttatgagatc aactttttta gcttccacat 26280atgagtaaaa acatgccata ttcatctttc tttgcctggc ttctttaact taagataatg 26340
tcctccaggc tcatccacgg tgctgcaaat gacagaattt tactcttttt taatcactaa 26400atggtatttc attgtgtata tatatcacat tttctttatc cattcatctg ctcatggaaa 26460cgtaggttga ttccacatct tggctattgt gaatagtgta gcagtgaaca tgggagttct 26520gaatcttttc aacatacgga tttttttcct ttggatatac acccagtagt ggaattgctg 26580gatcatatgg tagttctgtt tttagttttt tcaggagcct ccattctatt ttccacaatg 26640gttgtactaa ttcacattcc catcaacagt gtagttctac tttctccaca tcttcaatag 26700catttatttt ttgtcttttt gttatgtata ctaatctttt cagagaacca atttttggct 26760ccctgataac ctctattgaa tgtctgcttt ccatttcatt ggtatctact cttattttta 26820tttcctttct actacatatt ttgcatttaa tttgtggttt tctttctagc ttcttgagtt 26880gtaagtctag ataatttact ttcagtgttt tttttttcag tatatgcatt tatgtgtatg 26940caaaaggaga tgcattttgt ggaagtactg cgttagcagc attctaccga ttttgttatg 27000tcaaatttca ttttcatttt atggaaaata ttttctgat ttccattgtga ctagttcttt 27060gacccatagt ttatttagaa gtcttgtttt tttgattgcc aaacatttgg gaatttctta 27120gttgtttgtt tttttttaat ttctcgttta atttttccat gaccagagaa ttaccttatc 27180attcagtcct tggaaatttg tttagacttg gtctgtggtc cagcatatta tctattttgg 27240taaatgttct atatgcactt gaaaagaata tgtattctat tgttgttgga tatgtttctg 27300tatgtatgcc aattaggtca ggttgattaa tcatggtttg tttgtttgtt tgttttgaga 27360cggagtctcg ctctgtcgcc caggctggag gacagtggtg tgatctacgc acactgcaac 27420cgccacctct cgggttcaag cgattctcct gcctcagcct cctgagtagc tgggattaca 27480ggtgcgtgcc accacgcccg gctaattttt gtatttttag tagagacggt atttcaccat 27540gttggtgagg ctggtctcga acacctgacc tcatgatcca cctgccttgg cctcccaaag 27600tgctgggatt acaggcatga gccaccgcgc ctggccgatt aatcatgttt tcaagttttt 27660ctatgtcagt gctgttttgg tctctacttg ttctatcagt tacagagagg tatgttagaa 27720cttactcttt caattgtgga tttgtatttt cccatttaac ttctgtcaat tttactttat 27780atattttgaa gctgtgttac tggatgcatt caaatttagg agtgttatga tttcctaatg 27840atttgaccct tttgccattt agaaatgctc ttttcatttc ttttaatact gttgccttaa 27900aaattacttt gtcaaatatt aaaatagcca ctccaacttt ttttgggaca ctggagagtg 27960tgcttgagat acatcttttt ctgtcctttt agcatgtatc ctttcattta aaattatgat 28020
attacagtta ttaaaggaag actttccttc agtatttctt gtaatattgg tctcttgaca 28080gtgaattatg tcagctttca tggatctaaa aacatcttta tttttgtctt cattcttaca 28140ggatactttt agaatccttt atgtgaatta tatcagcttt catggatcta aaaacatctt 28200tatttttgtc ttcattctta aaagatacat tcttaaaggt tacatgattc caggttactt 28260tcccccacct ctaaccccca tccctccact ctaccacctc cccctcccca ccccccagcc 28320cttttcaaca caaagaatgg aaactatcaa ttttattgtt cctttaagca tatctcttct 28380ctggctgctt taaagatttt ctctttatgc tttgctttta gcaatatgac catgctgtgc 28440ctaggtatgc ttttctttgg attttcctgc ttaaagttcc ctgagtttct tgaatctctc 28500agttgatttc tttcttcact tttgggaaat tgtcagtaat tatcttgtca tatattgctt 28560ttgttccatt cttttttctc tctttctggg attccaatta tatgaatatg agaccatctg 28620actttgtccc acatgtctcg ttctctgttt ggttcatccc atttttgttt ccgtgattga 28680attctgttat ttatttattt atttattttt ggcctgtttg caagtctgtg aaacctgttt 28740ttctgtaact tactctgctt ttaaaatcat atgagaagtt cttaatttca gatgtttatt 28800tttcagtttc agaatgtcca cttggttctt tttttataaa ttatgttgaa aattgccaat 28860tgcattattt tacacatatt gttaatctct gcatctcagg tacttgcctc ctaacgttaa 28920tatctgggtt ttctgtgagt ctgactaatt ttctggtttt ctcttgatta tgtgtctgcc 28980acatattttg cttcttttga tgtcccttaa tgtgtttact tagtgtcccc gaactagact 29040aacactgtgt atgaatgaac atgattttct ttcagatcat gtgttttctc ctagagaggt 29100gtgctctttg ctgtgtcaga cagatagaat gaggagtttg attatctcaa tccagtgagg 29160gattacatag accgctccct ccctctacta ggattctgtc ttcttacgga agttttctct 29220tcccttttga cccagcctct tttttttttt tttttttttt ttttttgaga tggagtcttg 29280ctctgtagcc caggctggag tgcagtggcg cgatcttggc tcttggctcg tcacaacctc 29340tgccttccag gttcaagcga ttctcctgcc tccgcctccc tagttgctgg gattgcaggc 29400gtgcaccacc atacccagct aattttttgc atttttagta gagacggggt ttcaccatgt 29460tggccaagct ggtctcgaac tcctgacctc aagtaatctg cccgcctcag cttcccaaag 29520tgctggtatt acaggtgtga gccaccgcgc ccagccttga cccagcctct ctatcatcct 29580tcagtgttcc agttacattt ctgaggagat ataccactgg gaaagctgtc tatgtatcta 29640gagcttttct agattccagt ctgtcatttc agcccacatg ggcatctaaa gcactactag 29700
tctcttgttt cctttattag agttcctctg cttaagccaa gctcagtcct tactcatgcc 29760caagctctgc agttaccccc atggaagaac aagccagtgg tctcagctgc cttggaagtg 29820ctcttccacc tctggatttt ttgttctttt agtcctcatt gcttccacag ctttcttacg 29880ttggttagtc tggtctttga agcaggcgca ttggcctgcc tcctatctaa tcacttactt 29940tagggaagtg gaagtcagaa gtttatcttt ataaaaagaa aaggcagata attttaggag 30000tgcttatttt tataagcata gtgatgtttt gaatagtttt atctgtgttg catactcata 30060gctataaagc atttttttcc tttctgatat gggcctgttt atcaaaatat gccccagatc 30120tggggcgagc aaacaaagac ccacagacca atgaggccca attcttgttt ttatgtagcc 30180tgtgaactaa aagtggtttt tacattttta aaaggttgaa aagaaaataa aaaaggaaga 30240gaatgttttg tgagcattat gtgaaatttg gaagttttat tgtaatatat gcacactcct 30300ctctttatgt gctgccgatg gctgctttcg tgctgtaacc attgagtagt tgcaacagag 30360atcttgtgac tcacaaagtg taaaatattt gctgtgtggc cctttgcaga aaaaatttcc 30420ctattcctgc tttacatcac aggctcagga attttttatt tcttattact aagagaaatt 30480aagcaaaaga ttgaaaccat tttattccag aagttctgta aatttggatt tattaaaatc 30540ttagcatttt agaggattta caaggaacct cagtctcctt catctcttta ttttgcaaat 30600gaagcagatt tgagtgaaag aggttttaaa attatgacta catttttggt ataaagtata 30660ctcagtaata agctgataga gaaactattg atctatatca ctgaataatt tgcaaaattg 30720atttgttctt atattgcaga agtatttgat ttttcttaag cagtgtagct cttaagaaaa 30780tatttttgga atttttaaag taaatatttt aagataaata tttaaggcat gttaatagcc 30840ttttcctttt attgtcaacc acaattcttt caaatctgat ggatatgaag ttatttttgt 30900gagaagactg aaagtcttac aggaccaatt ccagacattc ttcattaagc ttcgattgat 30960ttagaatata aagccctaaa tcatcagaaa ttccaagtta aattgttaat gtgtaagact 31020ggtttttaga gtttctcttt tatattccca tcctcagata ttgtgaaagg tttaagctta 31080gttctcaaat atagagatat aatggtgtgt tatattacta gcaaatggca tttttaaatt 31140ttccaatgtt gaatacaaat tttgtttata taattttcaa cttattactg gctcttttag 31200aaaacttcta cctcttcctc tgcaaaccaa atcaacaaca acaacaaaaa acacagtaaa 31260agtaatttat aattaatttg ggaatagcta gttcttggat ggcctgtact taagaattga 31320tgggttcaga ttcataagaa ttgttacaac caattaaatg ccatcatgca tagaaagaag 31380
tattttagaa atgtacgtaa agtcatttga actttaaaga agttccatta gataaaacag 31440acacaaacat tggtctctaa ttgggaaaat ttgagtcagt tataacttgt ctttaggaaa 31500tctatctgaa tttggattta tatattctac ttctctgaaa attggtttgg aataaatttt 31560gatgctttta taagttatat agaaggggct aacctcttag tagttatgta ttcctgttgc 31620tcaaatacct gatttcactc aggtaatctt taatataaag gaaagttggt ttcctaaata 31680gtatgaagaa tacgtattgt gttttattta tttatttgtt catctgatat ttattgacca 31740tccctgatgc ttcacattga tgcaggtgct gaaagtacac agttgaaata gacattcatc 31800atgccctcac aatgctagta gttgagtaga ttctataagc aggtatatgt atttatgttt 31860agcatttggc actgttaatc tttctctttc tctttacttt tatggcatac tatcttgtgg 31920aaatctcatc cacatacata gtttccatga cctcctttgt actaaagtct gtgcaaaata 31980atcccaagga taactcctta gtgggccttt ctggctagag atgctttaaa tgcactcatg 32040tcgataccat agatgtagat accttatttt aatctctgag taaggcatgt agctgtagct 32100ctcaacttcc tcatttctcc ccttcctcac tctttctctt atactttctc tttctcttaa 32160aatactcgtt gagtactata tcccaggaac ttttctggac agaggctaga gtagtgaaca 32220agaaagacag ggcccctgca ctattggagc ttttagcttg gcgaggaagc aatacattaa 32280acagttccat acataattac agttattggc tgtacaatga aacataaata tacgatgaat 32340gagagtgtat attagggaaa ctcatccagt gtggggagtt agacttaagt gacacttatg 32400ctgagacctc gaagataagt agtggttagc aggagaagac gtggtagaga atttcaggaa 32460gagggaaaac ctttgtgaaa gttgtgatga agcttaacag gttcagacaa ctgaaagaat 32520atccttcttg ctggagtatg atgaatcagg tggaaaagaa tgcaaaatga ggcaggagag 32580attgatggag gtcagttatt ggaagaggat cttttaaacg atgtcggtgc ttttggactt 32640tatcttaaga gaattgagaa gatactgatt tttaagctgg ggaattatcc actcaggttt 32700gtgtctttaa aagttgagtt aggttcctat gtgaagaatg gattgaaggg tatggatgtg 32760cagaaatgag ttaagctttt gcagtggtcc aggtaagagg tggtggtaac ctagcttatt 32820agcattgcag cagtaaagat ggtgtggaca gattcaataa ctgtttatga gatataattg 32880accagatttt gtgattattt tgatttcaag agtgaaactg aaggcaatgt caaggataac 32940attttcatct gcttgttact cttgtctatt tgttgacatt ttggtatttt gaagttacac 33000atcttcaaaa tcaaacccat tacttttttt ccagcaggct aactcttcct tctaacttct 33060
ctatttttaa gagtgacatt gtctacctcc aagtgaccta ggcttaaaat ttttctcttt 33120tgtcactgct gttactaaat tagttaccaa atcttaagga cttgacccct tcagcatctg 33180gaacaacctc ctccccttcg ttctccctgc attccctctt ccccccgact tcatcctttt 33240ttctttatta cagcagtagt atcctcttgt tagatcacta aactgtgctt tccattcaag 33300tctctgagct ccttggggac aagaactgtc tttttcattt tgtgtcttta ctactttgca 33360tctcagcatg gtgaacataa aacatattaa gtgaaatgaa aataaaatgt tttccttgct 33420tcttgtttct tgcaccataa ttcttattct ttatcacata tttttttctg tttctcaaaa 33480gttattcagg ggcttccttt tgcttaaaga ataatttggt cttaggacta tgtgataatg 33540aagtatccag actggctgct ggagacataa agtatatgag ctggggattt tcctatagta 33600gtttagtaaa aagtggctct taagtccctt tgcaccgtag ccctcactac caaaaagagc 33660agattttttt tcttggaaac atcactgaca tttgtaaaca tcccactatg gataaagtat 33720aaaacacttc atttgatgcc tttttaatgt tttaagttta tgtttttgca gaattcaaat 33780ttgtattatc atttaatata tattgtatat tgcttagctg ccccatgtta tcttctcaat 33840agctacttta tatgttagta tcttatatgt gtatgtaaag tatattgttt cacataaaga 33900aattttgcaa gaaaaagatc caaatgtcta ctttggaaat acccattttt gatagctttg 33960tatgtcctaa tatgaacatt catatttttt tcccattttt cttttaatat ttaggaataa 34020gaaaatttta aataagaaga aattgaaaag aaaacagaag agcaaatcaa aagtgaagac 34080aagaagcaag gtaaagctgc tggtaattga taaggaatag gactttttca tatggtgatt 34140tgaataaaaa tgccaagatt aaaaaatata attaaaccat tttattagta tgtgctgaag 34200taattttgta aaagcccttt atgatttggt tcctctttta gtttcatctt tcttttgggt 34260gtttcttttc tttcttgcct tttttttttc ttttttgaaa cagggtcttg ttttgtcacc 34320caggctggag tccagtagtt tcctgggctc aagtgatcct tccacctcag cctcctgagt 34380agctgggact acaagtaaat gccaccacaa ccagctaatt gttaaatttt ttgtagagac 34440agggtctcac ggtattggcc aggcttgtct cgaactcgtg ggctcaagca atcctctcac 34500ctcggcctcc caaagtactg acattgcaga catgagccac tgtgcccagc ctatttctgg 34560gattttcttg accagtagcc tggaagttaa tatctggatg ttgaaaatta tttgctattt 34620attcttatta tctttgatca gtcctttggg gaacatatcc atttctgttc tcttttttca 34680atctgactac aggtactaga aacttgagta aaattagctt gtaaactggg acttggattt 34740
gggttcatca tatgcctttc ttgacaggaa ttagggaaac ttggtgtgtc catattgttc 34800tgagcctctt ttgactgttg gtagctctgc aaaagaaaag gcaatatgga atatttgaat 34860taggtgagac cttagaaacc aagaatctat tttcacctaa tgcagagatc tctataaaat 34920ccatcttttt tatactgaac attttcagtg accagagcac ttgatataac agtctctttt 34980ctattgaaca tctagacaga aggaagagag gagtcgtaac tatgattcct gaatagcttc 35040actttgagtt aaagaaacaa tagatttgct gggcctgtat agcaactata gaggcaattg 35100tttcctacac ttttaagtta gtgtttttac tattgtaact gagagaaatc tacttcaaag 35160tagcaaacct aaaataaaat gaaaggttgg gggatggcat ttatttggtg gctctatctt 35220gagacacaga tggatccggg gatgaaatga tatcattggc tgggtgcagt ggctcacgcc 35280tgtaatcccc gcactttggg aggccgaggc tggcagatca cgaggtcaag aggtcgagac 35340catcctggcc aacatggtga aaccctgtct ctacgaaaaa tacaaaaatt agctgggcgt 35400ggtggtgggt gcctgtagtc ccagctactc aggaggctga ggcaggagat tgcttgaacc 35460cgggaggagc aggttgcagt gagctgagat cgcaccactg cactccagcc tggcgacaga 35520gtaagactcc gtctcaaaaa taaataaata aataaaaata atcaaaccca tattttcagc 35580tcttgcttct gccttcctct gtgtgtggac ttattacctg tgactgaggc tgaggctgcc 35640agtagctcca actccatttg ctctgagcaa gaggcaatct cttatactag tcatagcaga 35700aagtcttggg taggattttc ggtttacctg catcatttgc tttgaccacg gagatacggg 35760atactttggt tagactgagt tatattttta accctgagat gggcagtggg tgagcaccct 35820ggtgaatagg ccacatgaaa tgagggagga atagttaccc taaggaaagt gttgccgggt 35880aggcagaaaa ataatctgtt cactgtatcc cttctcatta cttctgcttc tgggatagag 35940gagggaagtg gagcgcattg tcaatataca gaaaacctct aacaaataca gtcattccaa 36000cctctatccc ttttcaactt tttggtgcta tgtgcaaatc ggagtatggg aatcttgaat 36060atcttttaag tttttttaag gtttcatttg gtgattttcc tgaagtggtg gcatatgata 36120tccaggaaga ggtgaggaga agtcaaggtc ttttgtcccc ttcaattcgg cctccaacta 36180gctttgtgcc cttagtaatt cagttttttt cctcttgctc tcagtatttc tatatcttct 36240ttttaagagt agtttgagat aacagtgatg taagtaagtt cattcattta ttcctcaaac 36300attaattgca tgctgagtat gtgcaggcat tatgctggat gtttgggata caaagatgag 36360taagaggcat tttttgctca tggaaagcat ataaagtagt aaaatggtgt tgttagtctc 36420
cttgacctgt attacttttt ccagattatt tttcatctac cctctttctg aaagttattt 36480ttaaaatgcc atttagctgg cattttatct actttatatc tactttatat atattttata 36540tatatgtata tatatatgta tatatatata tatatatata tatagccatt atatctactt 36600tatggctatc atcaacccct ctatttctcc tcatccatca gtcatctcct ttatttaatt 36660tccatcttac ccaggttaga taccagtctt tctttttaga aaaactctta tttaatctgt 36720aacaaattac aaaaacttga tggtttaaat taacactcgt tatcttacag ttctggaaat 36780ctgaagtcca aaatggatct cactgagcta aaatcaaggt gtcagcaagg ctttattcct 36840tctggaggct ctaggaggga atctgttttt ttgcctttcc agtttctata gtagaggctg 36900ctcagatgtc ttgggttgtg gcccccttcc atcttcaaag ccagtggcgg ttggttgagt 36960tttctcatgc ggcatcactg atagttttct gttgttttat ctcctcctct tactgtccag 37020cctccctctt tcacttgtaa ggacccttgt gattacattg gcccatccag ataatctgtg 37080catttcaaaa tccttagttt aatcacatat acagagtccc ttttgccatg taaggtaata 37140ttcacctggg ttctggggaa ttagggtata gaagtttttg gaggctatta tttacctacc 37200atatctccct tgtatatttg ttgcattaat ctgacaaaac tcaatcttgt gtgaatccag 37260ttattttgtc cctcccttaa ggtgagttcc atgtgctgcc aaagagagtt acacaagagt 37320taactgataa tgatacacat tcatgatcat caatctcaag tataccatag ccagactatc 37380ttattatttt ttctggtcaa atagttctct tttcttctgt gattatttca gacctttgcc 37440atgctcctca aacctctgat aaattctacc ttccagcaca tgacttcact ttcttacagg 37500agaaatcaaa ttcattactc agaaaagttc ctcaacttcc taatcccaaa tgcgtctgtc 37560ttcaggcttc tcagtagctg agcctgacat agagatttga agtttgcagt tcagtaagtt 37620ttgaaagtta acattcatgc atagaagcac tactgcagtc aaagttcaga acatttctat 37680catctccagg ggttttcttt ttttttcttt cttttttttt tttttttttt gagacggagt 37740cttgctctgt ctcccaggct ggagtgcagt ggtgtgcgat ctcagctcac tgcagcttct 37800acctcctggg ttcaagcaat tccctgcctc agcctcctga gtagctggga ttacaggcac 37860cagccaccac gtccagctaa tttttgtatt tttagtagag acaagatttc accatgttgg 37920ccaggctgtc tcaaactcct gacctcaggt gaaccacccg ccttggcctc ccaaagtgct 37980gggattacag gcacaagcca ccgtgcctgg ccggttttct taccctctgc tgattccatc 38040cctccctata accctggctg tagacaacca cagatatatt ttctgtcact gtagattagt 38100
tttcattttc tagaacttca tataaatgtg atcatattgt gagtgctctt ttttttttaa 38160atctggtgtc tttccctcag cataatgatt tgtgttaatg ttgttgtata tcaatagttc 38220attccatttt tttgctgatt ttatatacat atatatgcac ttgtgtgtgt atgtgtgtgt 38280gttcagtttt cttgggtgca tactgaggag tggaatgttt aggtggtttt tcctttgatt 38340tgccagaagg actcataaat ctcacaagca atttatactc atggctaaga tttattacaa 38400caaaggatac agagcaagca gcaggaaaaa ggtatgtgtt tgtgatgtat aggaagttat 38460ggcataggct tcctagttct tcattgactg agctttcatg tcagaaagaa agacatgctt 38520tctctctggc agtgaactac agagaaatgt gtatattgtt tttgctcagg tgagttttag 38580aattcaagac tttgtgggtt tggtcacaca gacatatctt gttagtcaat cagacatggt 38640aagtgaaact caggtacaca ttgtaaagct tgatgttcgt acatgcagag cagtctgaca 38700ggccaggagg catggtccat tgctctgtgt gttcataaca caatcatcaa tcactaccac 38760aaagaaaact ctaaacatcc acattcccaa aggttagcca agggtcaatc atggtttcct 38820tggggaaata caaggagtaa gcaatcatgc ctgcagcgtt aactttttcc tcagagtggg 38880cacggtaaat gtacgtttag tttagctttt tttttttttt tgagacagag tttcgctctt 38940gttccgcagg ttagagtgca atggcacgat ctcggctcat tgcaacctcc gcctcccagg 39000ttcaagcaat tctcctgcct caggctcccg aggagctggg attacaggca tgcaccgcca 39060cgcccggcta attttgtatt tttagtagag acggggtttc tccatgtgga ggctggtctc 39120gaactcctga cctcaggtga tctgcccgcc ttggcctccc aaagtgctgg gattacaggc 39180gtgagtcact gcacccggcc tgtacattta gctttttaag aaactagaaa actcttttcc 39240aaagtggttg ttccaatctg tagtcccacc agtggtatat gaccaatcta gttgcttcat 39300atctttgcaa acatatggta ttcgcttttt aaattttagt cagcctacta agtaggggtt 39360tcccattgtg gttttagctt gcttttctct aatgactaat gatgtgaaat cttttcatgt 39420accttttgcc attcttattt ttttgatgtg aaatgtctgt tcagatcttg cctcttattt 39480ttgtaattgt cttcttctta ttgaattgcc agagtttctc atatattcta aatacaagtc 39540ttttgtcaga tatctgcatt ccaaatacat ttctcctgtt ctgtggcttg cttttggggg 39600aagaattgcc attttaatga tattgaattt tccaatccat gaacatggta tatgtctcca 39660tttatttaag tctttaaatt tcactcagca acactttttt gttttcagtg tgtagggctt 39720acacatattt tattactttt atttacaatt attatatatt tttgatgtta ctgtaaatgc 39780
agttattttt gaaaatttat tttgctttga gatgatttca gtatgaatgc agtgtgtatg 39840tgtttgtgtg cacctaagtc tgtgtaattt tatcacatgt gtaggtccat gtgactctta 39900ccacagtctg gatatacagc agttccatca caaggattct ctgtgatatc ctttatagcc 39960acaggtatct tccttcctca ccctctcctt agcttctggc aaccactgat ctgttcagca 40020tttataattt tgccatttta agaatgttgt ccagtgaaat tgtacagtat gtaatcattt 40080aagattggtg ctttttttcc ccacttggca aaatgtcttt gagattcaca cacattgttg 40140catattgttt gttccttgat gagtaagcat ttcatggtat ggatgtacta tagtttgttt 40200aaccatttac ccattaaaag atatctgagt tgtttctagc ttttgactat tttaaaagaa 40260tgctattacg aacattcgtg tactggtttt tgtgtgaaca taagttttta tttctctggt 40320gtaaatgtcc aagaacataa ttgctggatt atatggtaag cacatattta gttttgtagg 40380aaattgctgt actttcttcc agagtggctg taccatttta catgaccatc agcaatgtat 40440aagtttctcc tcatccccac cagcatttgg tgttgtcact atttatttta gccattctga 40500taggtgtgta ctatatctca ttttaatttg cattttccta gtagctaatg atgttgaaat 40560tcttttcatg tgcttattca ccctttgtgt aaatatcttc tatattgatg tgtttattcc 40620tatcttttgc ccatttgtta attggattcc ttgtttgttt ttactgttga cttttgagaa 40680ttctttatat attctgaata ctcatccttt gttggatatg tggtttgcaa atattttctc 40740ccagtctgtg acttcaatgc tattattttt gaaattacat tttttcagtt gttcattcct 40800agtatgtgga tatactactc atttttatat agtaatttta tatcctgtga ttttgttaaa 40860tctgcatact atttctggta acttttttgc agattcctta agattctcta catgtttttt 40920tacaagtaaa aacatttctt ttttagaaaa tacttcttgc ctttttccct tggctggata 40980ggatattttc cttgcactag ataggatatc cagtacaatg ttgatgagtt gctgagagta 41040gaccaatttg cctggatcac aatcttagag ggaaagcatt cagtctatta ccatgttagc 41100tgtggttatt ttgtagatac cctttatcaa gttgagaaaa ttcccttcta ttcctaactt 41160agactttttt tttttctatc atgaatcgat actggatttt tgtcaagtga tctttctgtg 41220tttatttgaa gtataatata ttttttctct gttacaacag tggttgcttt tccagtgttg 41280tgccagcatt gtccctgggt taaacctcag ttggtcataa tgtattactt ttttatatat 41340tgttggattt gaatagctaa tacttgtaat atctctggat atttggatga gatatttgta 41400atatctctta tacatatatt cataagttat atgccatgcc aatttaactt tttgatgtat 41460
ttcttattta acaagtgtat tttttttttc caattcttta tggaagaaga gtcgaatatt 41520tgggaccaag tgtttttggt acttgaggta tcattttgtt tatcttagat acttcctgga 41580catactctaa ttattggaag ttgaatttct aaaatatttt aaaacagctt tttatattta 41640taatataaat gttgatcttg aaattgtagt tgctgtctga tgaataaaat attgggcata 41700aaagagaaac tgtttagtct taacaaatta ccaatcacat gatttttagc ctttatcaat 41760actgataggt gagagagagc ttagggaagc atctacagtt tacttggcat tactgttact 41820tgaatgagaa tgaaatgagc tgatgaaaat taagtgtttt tttgggaggc cctcatttct 41880gtgtaaactt ctatatctac ttttaaaagc attcaaaatg caatctaatg tttgtagtag 41940gtcattgaga ctctacagtg tgtctagagt ctcttaggaa gtcgtaaaat gaatttcctt 42000tgatacagaa ctctaagagt taagctttgt tgagtctatt cctgtcatgg cgatacaaga 42060atatttctaa gttttttgcc catccttttc cagcccttgt cagattggtt ggttattgct 42120gcattgctca aaaaatagtg aggtatagaa aaggggacta gaagttgggt ccatagactt 42180agactgtctc tgctgtgtca cataatgtgt cttctgcaag tcagttagtg tgtctaatct 42240ttacttttat gtaaaatggg acttgatgat agaaaagagt gtgaaatgga aatattctgt 42300tttgtatttc agtctgaaaa cttagagaat acagtaatca taccagatat caaactacat 42360agcaatcctt ctgctttcaa tatttactgt aatgtacgcc attgcgttct ggaatggcag 42420aaaaaggaaa tatcattggc agccgcatct aagaactctg tgcagagtgg agaatcagat 42480agtgatgaag aagaggaatc caaagagccc cctatcaagc ttccaaaggt aagccactga 42540gttctattaa tatttagatg tgtaacctgc aggtgttctg gctataatgc atatatacgc 42600attgctaaaa tactttgctt tatgtaaaat tgcacactaa aaataacacg gcttatgggc 42660aaaaatagat ttggagcaga aaaggaaaac tctgcaactt tataactaag gtgctaacaa 42720agtcaataat tgataattca ctggaaagcg caggaaggga gatcagtggg ttggagtctg 42780ttataggggc aattaaaaaa tgtacaaaag cagtagggtg gaaatgctag tatagaggca 42840ttgagccatg gcagatggaa gtagagctct gagccagtga gccgagagta aggtgggcac 42900aagagaaagc caagagccct ggaaagctgg gaggtgctcc tcacctggga tgcaggtatg 42960cgtttttatt tttcttgaaa tagaactaca aagcctctca gctgtgtagt agcagggctg 43020agagcctttt cctttctgcc taagcttata attgtactct tgatattgtg gtttcccttg 43080attaggaaaa aaaaaaatca cctatgaacc aactgaactt ctgcattatt ctgatattac 43140
tcccttattt accaggagca tataaactag ttggtatttc tataatagaa gcatgtattg 43200taggccgggc gcagtggctc tcacacctgt aatcccagca ctttgggagg ccaaggcggg 43260tggatcacct gaggtcagga gtttgagaac agcctggcca acatggtgaa accctgtctc 43320tactaaaaat acaaaaatta gcccggcatt gtggcagtcg cctataatcc cagctactca 43380gaggctgagg caggaaaatt gcttgaaccc aggaggcgga ggttgcagtg agccgagatc 43440atgccattgc actccggcct gggtgacaga gtgagactgt ctcaaaaaaa aaaaaagaaa 43500ctggctgggc gtaatggctc acgcctgtaa tcccagctct ttgggaggcc aaggcgggcg 43560gatcacgagg tcaggagatc gagaccatcc tggctaacac ggtgaaaccc cgtctgtact 43620aaaaacacaa aaaaaactta gcccgtcacg gtggcgggcg cctgtagtcc cagctactcg 43680ggaggctgag gcaggagaat ggcgtgaacc cgggaggcgg agcttgcagt gagccgagat 43740ggcgccactg cactccagcc tggacaacag agctagactc catttaccaa aacaaaaaac 43800ctgtattata gaactactct cagtttactc tgttctgttt aagtattggt atatttaaga 43860ctggaaggtc tatatatata tatatatata tatatatatt tttttttttt tttttttttt 43920tttttttttt tttgagacgg agtctagctc tgttgcccag gctggagtgc agtggtgcca 43980tctcggctca ctgcaagctc cgcctcccag gttcaggcca ttctcctgcc tcagcctccc 44040aagtagctgg gactacaggc acctgccacc gcgcccggct aatttttttt tgtattttta 44100gtagagacgg ggtttcaccg tgttagccgg gatggtctcg atctcctgac ctcgtgatcc 44160gcctgcctcg gcctcccaac gtgctgggat tacaggcgtg agccactgca cccagcctgt 44220tatatatatt ttttctatat agtagatata gttaatattt acaacaaaag aataggtgaa 44280aatatagaca tttttaaatg gtgcctttat atgacttatg catattgctt ttttctaaac 44340agagctattc aaaatgattt tataattata aatgatttat gactattgcc tttttactaa 44400actgatacct taaaattatg ccttgaaagc ttatacatat ccacaaggga ttgtgtaaat 44460attttttact acttggaaat agcagcataa atggaacctg gtctgagttc ttttgcacat 44520tttatggtgg ctgttcctaa gtacaccacc atccttaatc atattgtcag gttcttctca 44580ccttattctc ggcagatggc ttgcttcata tttcatagag taatacaagt tttgtgaact 44640tactcagctt cttgatcagc catcttcaca tgtccttttt gcctcctgt tttagagtggg 44700acccttttct ttttcttatt tcctttgtct gcaatgtcct aactgtacct actctaatag 44760ctccttcctt ccttcctttg ctttcctttg ctttcctttc ctttcctttc ctttcctttc 44820
ctttcctttc ccttcctttc tccttttttc ttttcccttt ccttttccct tttcctttgc 44880ctttcccttt cctttccttt ctttcacgga gttttgctct tgctgtccag gctggagtgc 44940aatggcatga tcttggctta ctgaaacctg tgcctcctgg gttcaagcaa ttctcctgcc 45000tcagcctcct gagtagctgg gattacaggc atgtgccacc gcgcctggct aattttgtat 45060ttttattaga gatggggttt ctccatgttg gtaaagctgg tctcaaactc tggacctcag 45120gtgatctacc caccttggcc tcccaaagtg ctgggattac aggtgtgagt caccgtgcct 45180ggccaatagc ttctttcttt aacctaaaac aaacatgttt atctttaata tgggagctgc 45240tctacataga aattatggtc tctaattgta atttcttatg cttaactagg agagattgag 45300ataaaaaaca tagttgagtt aggacgtagg gctggaggac ttgtataatt tactttttag 45360ttagaatacc aattctgtgt atgtgtgagt cacactgtat tatagtaatt gtgctactta 45420agttcaatat tgtgagagaa aacaaaagcc tgggtaaatt ttttacacag gtatgcagat 45480tttgaatagt aaactgtagt tcaaattaaa ttgcataaac aagaacaatg gattcctagg 45540gtgtttaaaa acatcaggat tagtgagtat taaatgaaat atcaggcatt cttaagataa 45600ccttttgcag cataattaac agagtcaaag gggtatcttt caaagaaaat taaagaagat 45660cagatagccc aagtgattaa tactgaattt tctaccaagt actgacctgt ctgcaaacag 45720ggttagcatg ggttatggag ccaaactgct ggggttcaaa caccattaaa attactagct 45780atgtgacact ggaagttatt tgacacatcc gtgcctcagt tttgtcatct atagattggg 45840ggttgcatct acctcatagg gttgtagtga caatttaaat gaattaatgg atgttgagtg 45900cttgagagaa cagtacctgg cagaaagaag tgctcggtaa atgatatata tagttgtaag 45960tgtcagttta ggtgcaaaca agaaaatgtg taggtgcaaa tgaatttgag ttacttgtca 46020aggaactctt cagtttatta aaaaaattat gcaagcaaat gaaacttcta gagaatcagt 46080gcaatcacct gatgaatgag aggtaaagag aggaagaagt gatgagatgc ctctatgcag 46140aggcaagcag atactatggg tggggagtga aactttcagg tgatattttt tggcagccat 46200ttccatgaca tgaaggattg acactgagga cgaaattagt ctcttaaggt ttgaggtgat 46260gggatttgaa ggcaagtggg aaatgttggc atttttcttt tccctttgac cttctctgtg 46320cctcagcccc aaatgctgct attatcctag tttgctcctt ttctttgttc ctctgttgct 46380cctcctaagt gtgtggctgt cccattcctt cttgcctgag gtttctgttt cttgtactct 46440tttttttttt tccttaacat gattaagaca gtttttaatc tgttgttgaa gtgttgttgt 46500
aatacagttt tatttttccc attcttgagt tttaaattat tatttagtta ctaataaatt 46560gagtttgact gttatataga ccttgattgt cacccttttg ctttttgctt ttgtttttgt 46620gttttgcgga tcagtaatca ttgatgtatt taaacgagct ttctattccc tgaatgcttc 46680tacattaagt tttttaacca gctcaaactg tcactaaaat ctacatcctc taaaacttta 46740cagattttat agctacataa tttttattat ttttgttttt attttcattt tgagacaggg 46800tctggctctg tcactggggc tggagtgcag tggcgtgatc tcagctcacc gttaacttcc 46860gcctcctggg ctcaagtgat catcccacat tagcctccca tgtagctggg actacaggtg 46920tttgccacca ccccaggata ctttttgtat ttttagtaga gatggggttt tgccagattg 46980cccaggctgg tctcgaactc ttgagctcaa gcagtctgac cgcctcagcc ttccaaagta 47040ctgggattac aggcaggagt caccgtgcct ggtgcataat ttttaaatag taacgcttag 47100aagtcatatt tcccatctga acccatttga agccagtcat gccacattat taaaagattt 47160agacttacct aggatcattt ctttcttaat tcagtatatt ctttttgaat ttgtagccag 47220cattatatta ggtcctgaga acaaaaggat gagacataat tcctgccctc aagaaagcat 47280tgtcttgtag ggataagaac caggtcaata aatattctct agtgccttaa ggattgatag 47340ggagtgctag atatagtgga acacgttaga gaaaggactc aacactggct gagatagagc 47400aagagagaga gagcaggaag ctaaatcatg aagggcctta tgtgccatgt aattgattca 47460gatgttatct cataattagt aaatattgaa gattttaagc aaaagaatat catcagactt 47520gcatgattgc atcagactca ccaccctgta gttatgaata tgccttacta acactggagt 47580aactttgtac tccagtaagt aagtaacttt gtactccagt tagtaagtaa ctttgtactc 47640cggttagtaa ggcatatcca taactacagg gtggtgagtg agactggaga cagaggaacc 47700atttaggcag ttattgcagt tgtctagaga gtgttaatga gggttggaat tatggtagtg 47760acagtgtttt gaatatagga aatgttaagc agaattgaca ggaattagta ataaattggc 47820tataacgact gaaaaagaag gcagaatcta aagatattct taaaggtttt gtgtggtgag 47880ggcctttact gaaagaggga atactggatg aggaacagtg cttggctgaa atgatgatca 47940agtttagttc acatgctgat tctgaggtaa atgtgaagta tccagtgtgt gctatgtagg 48000gctgaagttc aaaaaataag tgtcagctag aaaaatagat ttgggagtta tcactatgta 48060ggcacaagtt gaaacaaatt tctgtgagag atcggagggg gtgggatcaa gaacattgtt 48120tgaaaaaata gctttgaaaa gttgaaaagt tttgaaaagt tctcttcctc tgagtgtgaa 48180
gagaagggaa gaagatggta gtagtcatag ataaatttgt taggcaagga actcagaaac 48240ttcacactca tactttcttt cttttttttt tttttttcaa gatggagttt cactctgctt 48300gcccaggctg aagtgcagtg gcacgatctt ggctcactgc agcctccacc tcctgggttc 48360aagtgattct cctgcctcag cctcccaagt agctgggatt acaggcatgc gccaccatgc 48420ctggctaatt ttgtattttt agtagagatg gggtttcacc atgttggcta ggctggtttg 48480caactcctga cctcaggtga tctcccaacc ttggcctccc aaagtgctgg gattataggt 48540gtgagctacc acacccggcc tcacattcag aattctccat cttagccttc tcctcctctc 48600tttctgctgc tgcctcttcc ccctcctttt ttctctgtct ctagattttt aaatttttta 48660ttatttgtag atattatctc tgtaccaaat atttgcttta tacaaacaat tttaaaagac 48720agtatacttc actctatggt aatatgtaaa tataattttg atttcctttt gagctttttc 48780aaatataaaa gtataggaag tgattttagg accttttaac taaacaagat acaattttta 48840agggtaaaga attaattatt aaatccaaat gtgatgtaac taaactttta tgatcacact 48900tagtcatgaa ataataaagt cacgtttgga aatatatgat ttgagaaggg caaaataaaa 48960ccttgaaaga ctctttatat atcatttttc tttggccaaa tatattttta tctcactttc 49020ctagcatatt agactggctg tatcaggtaa ttatttgggc ttcatgtttc tttatctttc 49080aaatatgata gataaccttc aaaatttaat tatttgagag cagcagtaaa ggtaaaattc 49140agtaaattta caaaagacta ttttctgaag agaagtgcga aagcatgtgt tgggtaatta 49200gttatagttc gttatggaaa gtattgaagc ttcttgtgtt cttttattgt tataattatt 49260atttttatta aatatcacat ttatttattt tgctaattat acatatttat tgtagatgat 49320ttgaaaaatc agaacatttt tctaaagagg tattaaaaat cggaagtaat atttgaaata 49380ccattaacca aaatataccc caatataaac atttttattt cttttgtcat tttttctatg 49440tacacattaa aatgtttttt acacacaaat gaaataatac tatattggca ggactatatt 49500ttttctcctt acactcttcc ttcctgatac tgaaggaggg agaaatatgt gttttcattg 49560actataagac ttcaaactgt tttaaagtct actttcaaag ttcaaacttt tggtaaaata 49620ctttatactt agccaaacag cagaaaaaga cttatttaag aacatgtctg ttacctctgt 49680gcaataatcc atagctaaat attaaaaatt ctttaatagt tgcagaactt gaggttgatt 49740tttgttgtgt attgaatata tattactatt ctgaaaatga atgttttccc atttgttaaa 49800caaaaagtct acaatgaacc aagatgtgga taattagtga gactctcaca gtgtttaacc 49860
cagacagatg aagagttccc tatcatcatt gtgaccaaag ttttatgggc cagtctcact 49920aaaaccggct agctctgttt ctaggcagcc cacctgagtt gcctattgcc attcacttgt 49980tattacacct taaacttacc agagagatca agaacctgtt cattaggcag tcttactgtt 50040tctggcaagg ttcaataatt ccgttgtcac cgtaggaaac tgtatctata ataaatgaac 50100cttagccaaa aatgtcatct gtgaaaaatc ctcttggatt tatgaaaaat catggttggt 50160agcagagtaa gcaatctgaa agaataacag aatatagtag tcttccattg gcttgagaat 50220agagccccat attttgtact ctggagatct ttgacttcac atttctcttt cctgtaagta 50280cataagcaga tggaaatctt ggacagcatg ttcttgtttt ctagtccatt ccaggaaaag 50340aaactatatc aaatgtgaaa gtttaatcac ttaatgtgtt taatcaattg aaactctatg 50400cagactcttc tgtaatatta gctataatca agtctgttag cattttagat aacttctcca 50460aagacagtcc tcctagttat ttgacttctt agctatatct gttgtttcca gaaaacttgt 50520aaatgttaat cagaactaca attgacttac cttcagccga cttgcttctg gtttagaaaa 50580tgtatttctg agatgcagtg gttttttagc aaacaattta gtgaataaat taaacaaata 50640tttaatgtgt gcctaccata tgctaggtaa ttggctaggc atgggaggta ttaagccgtt 50700taagacatgt tccttgtata ttggaagata atacaagccg tttaagacat gttccttgta 50760tattggaaga tcccaacatg gttgggaaaa tgaaaacaaa aatagttata ctgtaatgta 50820ataaatgcta taacagaggt ctgtattgag tatgaattat atagcatgac taatttaccc 50880tattagatta taaactctta gaggacaggg tctagtatac cttcatattt cctaaagtga 50940gtttgcacaa tatgagtgct taggaggtat ttatgtaata tatgaattaa tgcaaaaata 51000tcagcagaga gaatattctt ccactgttgt gttcaataga attaatggtt ttgggagttt 51060ctactaatta gtcttatttc aatttttctg ttacttctcc aaacaattta attaatactt 51120cttgaactgg ttctcagaat gttgacattg cctgagaaat ggaatttgtc ttttgtttta 51180tcagtcttat gaaggtatat ccttaaactc attcattgca atttgctctc tcaatcttta 51240gtaacaaatt tatctctttt gctggatata gagtctgatt tttcttgcac ctgatgagga 51300atagtaagat aattaaagga aaagtaccag tgatattttt agctaagcag ttttaaattg 51360ttttgtgatc atcctacaag aagggatacc aactataaat aataatatag tgattgtacc 51420attatagaaa aggctaagac gaaaagctcc ttgtaaaatc tttttactat atgtgctgtg 51480ttgactttat ttctggttat aggtaacaat taacatgact tttgacaaaa agagtactga 51540
attttccttg agtacaatca aatgaacttt gcaaattaat atagtttatt tcttctgagt 51600gaatgcaatc tagtctctag ttcattgtta taagccattt tcatgatgtt ttattatggc 51660tccattatac atatacatta tcatttgctg tggaagtaac ttgtaccaac taaactgctt 51720agaaagtgtt actagcttac atgtcataaa gtatatatat tttttccttt taaatagatt 51780attgaggttg gcctttgtga agtttttgaa ttgatcaaag agacacgatt ttctcatcca 51840tccctgtgtc tcaggagtct ccaagccctg ctcaacgtgc tgcagggcca gcagccagaa 51900ggcctccagt ctgagccacc tgaggtccta ggtaagagcc aaggctcatt cagtgaagca 51960tttaaaagtg aatataatta ataatagagt attgtacact tcaaaattgt taagagaata 52020tttctaaagt tctcactaca gaaaatgtta agtacttgag gtgatagatg ttaattagtc 52080cacattttat tcatgaatca taacatcact ttatacccca taaatttata taattataaa 52140ttgtcaattt gcagttttta aaaagtgaag agcttctctt atgaagcaca cacaatagcc 52200tgtaatgcag tctttattta attgtggtac ggaaacagca ggggtataag ttctagagta 52260tttttttctg tgttccactg aagttatggt ttaagagttg aagttcgtgc acacacattt 52320ctcaattagt atgatgaagc ggggggcaaa caaaacataa atccctggaa tccagaaata 52380atcctcaaac cttaaggtca ctattaactg aaatcgttct tattaaactt ttggccaaaa 52440tagctaacat tcactttctc taacttgtta ctctcaatag ttttaaaaaa caggggaaaa 52500ggaaataaat gccacaaagc caaaacagta caattagttt ctaaattgag gggacttttt 52560tttgtattca tcataaataa aaatctattt gtgttatgga ttaaacttct gacttagcaa 52620ctttcattaa gtaaataaag tgttcgcttt tcttaaagta tgttccgatt cttgcctttc 52680atatcagtga cccagactca acatgtaatc ctttaaataa gataggagct tttacttaaa 52740aggtactaag gaatagaaat atagatggaa gcataatttt taactggtta attgcttttt 52800ttctccgatg ctaactatgg gtctttaaac tatctaaagt tttctagatc cttctattat 52860tataggaaaa ttctcagaag tactagagct gtggttctca ttatgtggtt ctcaattctc 52920attagcatgt atcagagtta tcggggactt ccctatggtg tttctgattc catagacttg 52980gggtggggcc caaaagtttg tatttggaat tagtttcttg gtgatacaga tgctggtttg 53040aggagtacac tgctggttta gactaaaact tggtcctttt tttccgactt ggctatttag 53100attttgtagg acccacctgt gagaaccaaa ctgcatgaac ctaactacat gtcagatttt 53160tcttttttgg tcgcatgtgg ggactcagtt attgtgtttg acttttgttt gttttaatga 53220
gagggaggat gctggtcatt gaatcaagct gaggattttt taaaaccacc cccctcacat 53280acacagttat taaaagctat taccaaatag aagctgtcat gttggcaggc aagcaccgtg 53340ttggttattg ttatgatagc accagtgatc atattaatat tgtgaagtgc tgcttggctt 53400gacagcattt tactgaagat ttgatttatt ttatttaaca atattttgct cggttttctt 53460ggatttggtt tgttagttag caaataattg gaatgttgaa atacccgcga agacttggat 53520gtcgtatttc ctactttgaa catgttattt ctttcctcct aagtcttcta ccattctcat 53580gtctcatgtc agttgttata tactttcctt tgtatccatt ctgtacatac tttctttcct 53640ttattctttt aaaactagaa cttgttctta atactggctt tggcatataa tatgtgcttt 53700agtcttatct tccatcaaaa ctttcacatt attggctggg tgtggtggct tatgcctgta 53760atcccagcac tttgggaggc tgaggcagga ggatagctta aggctgggag tttgggacca 53820gtctgggcaa cttagacccc tatctctcca aaaaaaaaaa aaaaaaaaaa aactgggcat 53880ggaaatgagt gctactcaag aggctaaggt gggaggaccg ctcgagccca ggagtctgag 53940gctacagtcg gctgtgatca tgccactgcg ctgcactcta gctgggtgac agaatgagac 54000ctcatctcta ccaaaaaaaa aacccccaaa aaatcccaca aaacccaaaa atatttcacc 54060ttattaatct gttcattttt attttcttgt ttaaagtttc aaattctgct gtactgcctt 54120tcgtctgtct tccatccttt cagtgtttac tgctgatctc gtcactgctt tggcttattt 54180ttctatgtaa gggatttatt atcacttcat gttttctatt ttgttaaatt agctcaaaga 54240taaaagcact gatttggttc ttttatacta aatttctttt gaaacttgcc tctcctcctt 54300tttttaatct aatatcacaa tcttattggt tatccttttt ttcccattcg gttgttttcg 54360tgttattcat gaaaatatgt gtatatttat ataactctta ctgtggaaca tcttgatgat 54420tagtatacac caagcttctt ctctttgcaa atagattttg atttttaaat tatctattta 54480aaaatatatt tccttatatc catatattct gttcaattag attatattct cgagtactca 54540ctgaaatgta atagcacccc ctttgttatg tgctaagaat gtgcgatact attgctgtta 54600ttatggaact tagcatcaga gagttggata tgtgtataat tacaaatgtg gataaatcgt 54660atggaggaaa gtagaaggag ttctgagagt ccttttttgt tttttgagac ggagtctcgc 54720tctgttgccc aggctggaat gcagtggtgc gatcttggct cactgcaact tccacctcct 54780gggttcaagc tattctcctg cctcagcttc ctgagtagct tggactacag gcgcgttcca 54840caatgcccgg ctaatttttg tatttttagt agagacaggg tttcactatg ttggccaggc 54900
tggtctcaaa ctcctgacct caggtgattt gcccacctca gcctcccaaa atgctaggat 54960tacaggcatg agccactgcg cctggccaag attccttttt taacttaaaa attttttatt 55020tatttatctt tagggacagg atcttactat attgcccagg gtagcctcaa accctggact 55080caagtgatcc tcctgcctcc acctcccaag tagctgtgac tataggtaca cactgctgca 55140cccagctcct tctgagagtc tttaacaggc tccctgaaga tgtgacatat gagatttaga 55200gggtgaatag gagggagtga attcgattat aagtgctcta ggcagaaata acagcattta 55260gtaaggctct gagacagaaa ggagaatgaa tgaaaagaag agcaatatgg ctagaatgtg 55320gagagtgaag gaaacagtct tgtgacaggt gaggaaggag gcagggacat gcagagcttt 55380acatgccatg ttaaagaatt tgattagtgg gctaggagga atggaaagtc attctctatt 55440tcattacatg aagaatattg ttttttaaat agccttattt tcttaaatta tttgtttaaa 55500ctattgacta ttttctttcc ctggattctt aacctttttg aaagggtaga gacattcttt 55560gtcatgtttg ccaataccct agtgtagaat agttgccagt gtgtgagagt tcggtatata 55620tttgctgaat aatcaacaga tgtactcact gaaggataat aattggagga aggatgggta 55680gatggaaata tggacaaaca gggatagatg aaaggatgga gggaaggaag gatagctgga 55740ggaaaaggta gaggaaagga taaatggaaa aaaatggaag tataggatgt gtacagacaa 55800gtgaagagag gaagggggaa agaaaagaag acagaaggga ggaagaaatt aacaaaggag 55860tgaaagaatt ttaagtagac aatcagattt ttggtttgaa attctccctg gctattgggg 55920caaaagcgaa tggtaagaga taatttctta acacttttta tcaaagagat gatgatagca 55980tggattggaa agatgatgat gagataggga aaagtggagt tatccattag gaagtaaaat 56040aaacaggact taatttggga ttagctatgg aagatgaggg attggtaggg gctgcctggg 56100tttctgtaag ttaccagata acccacaggt ggcattttag tttttagaga attgcagtac 56160attactcctc tttctcttgt tctcctcctt ctctcccttc cctttccctc tcttcatgtt 56220tctcagtttt tctttttcta actctctttt tccctctcca tctgattatt cactctataa 56280ggattttcaa gtctccgttc tatggaagaa atacccagat gtctgttgat tgaatcttag 56340atggaggtgt tcagggaatt ttgtgcagag acctctgctg gttgtaaaac atttgtgtgt 56400gtgtgtgttt catcttcacc actaaatgac tttatgattt aggaaaaatc ttttgatacc 56460acatatctca atttcattat taaaggggga gaatatctgt cctattgact tggaggtttt 56520tctagggtat aaatgaatta catcctaagt gtgaattgct attatgttaa tgatatagcc 56580
aattttatat taaagcacat tattgcatat gttttatgtt tctattactt tgaaaatata 56640tacataattt cctggacact taacatttta agaagcctcc taatctaaaa ggtaaaataa 56700gtatgacaag tagtctatta cttgatagga tgagtttctt gaatatgtga atgcctctag 56760gcaagcttgt tttaaaagac cttagattgt ctttttttac taagaaaaat aaggagttga 56820actgaatgtt ctttaagacc cttgttacat aaatatttta acttttgggg aagagagtaa 56880ctagaaagaa actaaagcaa agtaattgtt ctagattcct taaatttcgt ttataatgag 56940acaaagtaaa aataaataaa tatacgcata taatgttccg tatgttggat gaatataatt 57000gaattctatt ttatccacag agtctctctt ccagcttctt ttggaaatca ccgttcgaag 57060tactgggatg aatgacagca caggacagtc cttaacagca ctttcctgtg cttgcctctt 57120tagtctggtg gcttcttggg gagaaacagg aaggacactt caggccatct ctgctatcct 57180caccaacaat ggaagccatg cttgccaaac tattcaggta tttcatattt atatctgctt 57240agaagtttat aagatgacaa gttacagttt cgtctcaatt tctgtcaata ggaattctgc 57300tttttctcac tcattacagt agcttaatct gtccaagatt gatacataaa taatctacag 57360tatttcaatt aagtgatatg tatgtttaac tctgaagctt taaatataat aaaatgaatt 57420ttggccagac ctcttaaatc tttaagctgg tctttgcctt aacaccatag ccttctcttt 57480ccttgctgtt ttcttgctgt ctgcgttctc ccttcttccc atctttccct gagtttttta 57540gtatggaatt cattcatggt tttaaatatc ttttctctat tgaaagaaaa agatatggta 57600tcaatctgat atatttttga ttggttgatt atgatttttt tatgaattga ttcgtttctg 57660agttacttca gctgtgtata aaacatttta ggcttttaat attcagttgt ggaacaaaca 57720agctcatcac cattttagtg gtgggaaaaa taatccttat aggcaaactt gacttaatta 57780tttgggaagg ctctggaaat tgagaaagaa gttatgatct taagacctga ctgtctactc 57840ttctctggcc ccttgggcaa ccctagggca ggtggaaggg atgcctcaat cctgttatta 57900gcagaccaaa gatagcaaaa ggagtatatt ggtcattttc tgtcttattc agtgattttg 57960aactctcctg gtcaaacaag acccattccc ttactcaaga tttctgctct tttcttcctt 58020tctgccttgc ttggaatcct tttgtccctt tttatggtcc ttaattattt tgaagttgct 58080ctaatacagt ggttttcaag ctgtgttctg tagacatgct ttcagggttt ggcaaatgtt 58140taatttaaat atatgtttaa aatatgcaaa tattttaaaa attgtaataa agtaacacaa 58200acattcagat accatatacc agattttaat aggatagtga ccaccaacat atgagcttat 58260
gaattttgat atgatttgct tttatataga gacttaggaa tgccattaga gctcatagca 58320ggagtataag aactacgttc atggaaagcc tcccagagga tatgaggtga gatctaaact 58380aatgagttga aatcagccag gagaaatttt tggtgttggt ggtgtagaga gagaatgttc 58440taggtatcca catgctaagg catggaggtg agagtaagag tgaaatattt gaaagctgaa 58500agaagtttag tatagctata gcatagagag ctgtcaaata gcgtagccac agcatacaga 58560gctggcaaat aacagagaca ggactcgagg gcttatctgt ctccaatatt tatgctcaat 58620cactagacaa cacaaaacta cttgatcctc tgaatcaata gctgcagttc tctacagacc 58680tgccacatca ccacatcccc aactctagcc taaactccat attaaagccc attaccttaa 58740catggcttgc agtgctcttc atgatttggc cttttatcta ccaacttcat ttcccaaaac 58800tctcctggcc tgactgttgg tagagggaga aaagctttcc ctgagaatgt gtgcattaag 58860tccgttttca cgctgctgat agagatgtac ccgagactgg gcaatttaca aaagaaagag 58920gtttaatgga tttatagttc cacatggctg gagaaacctc acaatcatgg tggaaggcaa 58980ggaggagcaa gtcatgtctt acgtggatgg cagcaggcaa agagagcttg ttcagggaaa 59040ttcctcttta taaaaccatc agatctcgtg aggcttattc gctatcatga gaacagcaca 59100ggaaagactt gcccccatga tccagttacc tcccactggg tcccttccac aacacgtggg 59160aattcaagat gagatttgag tgggaacaca accaaaccat atgagtatgt gtaactcttc 59220acatctcaga tttgttttgg cacccaaatg catactactt gtccatttca taaagcttaa 59280cagagagttt aaagcagggt agacatggta gtactgcaag atgctgtatt gaaagacact 59340gaaatccttt ctggagggtt gaactcttga cttcaaaggc tttctgcaaa taaaacacta 59400tttattgcat ttgagctcag aaacaaatta ataaaaaaag cacatggaaa aaggcaccat 59460tattgagaat tagcagaaac aaatatggta gaatcaatcc tgccaagtcc ttagatattg 59520aaattatttg atatagaatg tgtatgtgta tgaatatata aagtatggtt aaagtaataa 59580aagaaaatat caaaagtatg attaagtagt gagaatataa aaagtagtca gacattagga 59640aaaaaaacaa atcgaactcc tagaaatgaa aaaccaaata attacaatta gaaacttaaa 59700atgggttaac tattgtataa tatggttgaa gatggaaatg gtgaactgaa agataaagag 59760gaagaaatta tctagaatgc aacagagaga caaggagaga aatatggaag ataaaataag 59820aaacatggat cgagaatgac aaaatctaat gtaatacaat ctgagttcca caaggcaata 59880atagatttgt ggagatagag tacttgaaat aatggctaag attttacaga attggtgaat 59940
gacatggaat cccaagagta aggaagtcta ccttgcttga gatttgcttc ttatagatga 60000acttttagtt ggtacttttt gctggcatgc gaaatatcat tctgaacaat ttattgtctt 60060atgaatgatg atgtattacc aaagtatctt tgttctgtgt ttgtaacatt taaaaatgtt 60120ttttcattat ttttcttctc ttttgattag gtgccaacaa ttctaaattc gctacagaga 60180agtgtacaag cagttttggt gggaaaaatt caaattcagg actggtttag taatggcatt 60240aagaaagcag ctttaatgca caagtggcca ttaaaagaaa tatctgttga tgaagatgac 60300caatgtctac ttcagaatga tggatttttt ctttatctat tatgcaagga tggattatat 60360aaaataggct ctggatacag tggaacagtt agggtaatgt gattccttac agttccttaa 60420ttatacagag ttataaccat aatgaattgt gttctgtgtg tttctagttt catttctaga 60480atatgatcta attttagtgt aataatattt atttgaaaac cataattgaa atacatgcat 60540taaatgtcat tcacccattt gtattatttt tcattgatta ttaattatga aaccactgat 60600atactaactt ttgttttttt tgcagggcca tatatacaat tctacatccc gtattagaaa 60660cagaaaagaa aaaaagtctt ggttagggta tgctcaggta agaaactatc cacaataaac 60720caaaattttc ttatctttta caacatgtga tttgtccttg tttaatcagt aatatcactt 60780ctatttaaac agaaatgata atatattttt actaatatgg ccataataac tgaatagtgt 60840agcgtaaaga acactgtctc agaactcata agaggttcaa ttttagttct ggccctgctt 60900cctactagct gtatgactgg acaactcagt taacttattg ggtcctctgt tatttagctt 60960taatatggga ttaaacatac cttctttaga gttcttgtgt aagtgagcct ctagataaga 61020attgaagacc acctttatca ttagcacatt ttttttttta attgagacag aatctcgctg 61080tgtcacccag gttagagtgg agtggcacag tctcggctca ctgcaacctc tgcctcctga 61140gttcaagtga ttcttgtgcc tcagcttcct gagaaggccc atgccaccat gcctagctaa 61200tttttttgta tttttagtag agacagggtt ttgtcatttt ggccaggttg gtctcaaacc 61260cctgacctga agtgagccac ccgcctgggc ctcccagagt gctgggatta caggcctgag 61320ccaccccacc cagcctcatt agcacattta aattggaaat gtatacatgt cctgaggcca 61380aattagggtt tcttaccaca gactctttca ttttagtcta gattcagtac tttctaggaa 61440acagtgtttg gcctactcag tagacagcta cctgcatgaa gaatatgcaa agaatcacaa 61500gagagaaaga aaagccctgg gtttttgtgt cttaatgctg tgatcttatc tcctgaccaa 61560catagtcaga taggtgttag ccttttacaa ggtcagcagc cagatggata cattactcct 61620
cttatggaga aaagcaaatg aaggatggac aagagggact agaaatattt ttttccatac 61680agtacctacc tgaacagtga agcccaagtt cctttgtata aggataaaga ataaagattt 61740ccctgcatgc ctgccttgct gagcttgtga gatttgttgt caggatcaaa tgataaaaga 61800tatttgaaag tcttttgaaa actatggaaa ttactattgt tacatgaaga ataattctac 61860tgtatcacct attggggaga atttgaaatt aaattttttt ttttttttgg agttgtagtt 61920ttgctcttgt cgctgaggct ggaatgcagt ggcgtgatct cagctcactg caacctccgc 61980ctcccaggtt caggcgattc tcttgcctca gcctcccgag tagctgggat tacaggtgcc 62040caccaccacg tccagctaat ttttgtattt ttagtagaga cgggtttaac catgccggcc 62100aggcttgtct cgaactctta atcccaggtg atccacctgc ctcagcctcc caaagtgctg 62160ggattacagg cgtgagccac cgcccccagc ctgaaattaa attttagata acaaattagt 62220ttctcagtaa ttgtctctta aaaattgaac ttagtttaaa atatctcatc agatttttat 62280ttgccactct attgtgtttt atctaagaaa taatgccgtg gaaagtatat tattatagca 62340gtgtgttcaa tggcatacca caccttctga aaccactatg ctattcattt tcaaatagca 62400aactcaatac ttgttatttt tcttaaagta cttgttaaag taacagtgat cattcatatt 62460atttacttgg caagtggtat ggtcattttg aacaaaatgt ttgtaactgt actgtcctgt 62520ttagtatacc taattatgtt tctgggaata tgttacatca attttcaata atgtatgact 62580tttcctctaa ttgagaggaa tgttcttata tttttaatca ttaatatctt ttagacatca 62640atattgatgt attattgtac ctcaaaaacc tgtaatatgg agctgtatgg ctgctgtttc 62700tgctgaatta gtaataaata ttttaacagg aaaatttttt gctgttatca gggttattta 62760ttatatagag atgtgaataa ccacagcatg acagccataa ggataagccc tgaaacactg 62820gagcaagatg gtactgtgat gttaccaggt atgtttcaag tagtcatttt ttctccacaa 62880gcaattttaa gaaatgtgca tgttaagcta tttagacaca taaagaatga ttagcaaggg 62940atagtgcttg cttataaaag ggttttaaaa atcttgacat acaaagcatt ttatagtcta 63000cgtgaagtta atacatatca agagaataga catagaaata taacaagttt taatatttgt 63060tttcaaactt ggccgtctat ggcctaatct ctgacctcac cccaccactg ctgaattagt 63120ggggtgatgt aactgtgttg ctcttaataa aaatcagaac tctggctctc atgtgactca 63180gtataaagaa ccttctgttt atttttactt taacaacagt gcagttgggt tttcctcctt 63240atttaattta atttaatttt atttaattta atttaatttt attttatttt attttatgtt 63300
aaaggggttt cagagacaga gagtttaggg taggtcagga attttttttt ttaatttcat 63360catttatgct tcttatgctt cttaatactt tgataaaggt ctttggacac agtttatttt 63420gctttaattt tgaggctttt atatattcac ttaaatcact gctaaattat caggcagtag 63480gtctccaatt ttgatgaatg ctggaaaaat gcatattctt tattgtaaga tctttgaatt 63540tgtttataat gttgatactt tctgaggctg tttatattaa aatcttgagt gtctaatgtg 63600tctgtcaata atttcatagt agcaatatta cttgtaagcg tttgaccatc tgaaagtgag 63660agctacttct gaagagtgca ttgagaagaa taattgctcc tgggctgcat gtttattttt 63720aaatataatc ttaataattt catcaaacat ttatttgaac cttactacat atacatagat 63780acatattagt gtacatgaca ctatgttaaa gtaaatatga catttcatgt ccatttcaga 63840aatagaggag ctttggaaag cggatgactt aataaaatca ttttgaaaat aaccatctat 63900tttcagatat gtctacaggg tttttttttt tttttttttt ttcattacaa gtcctggacc 63960atgtctttgg tcataataat aataataata atgacaataa tattaaaaaa tacttttaat 64020aacatgcaca ctccccccac atatttatac atatattgaa agtaatcatg tttgttgtat 64080aaaaatcata tgaacaaaaa agaatgaatt gaaataatta taatcccact tcccatatat 64140aaacactatg aacatattgg taaatatcct tctcgtattt ttcctttgtg tatatgtcca 64200tacttgtaaa aaagtgtgat catatgctac atattgcttt tttaatctat ctctttcact 64260taatattctg aaaatatttc taggtcatta aatagtcttc tacaatgtca cttaaatgtt 64320acaaaatata ttccattgat taatgtttga gaatgaaagc cttccagggt tttgctatat 64380aaattacact gtaatgagcg ttcctatcgt gctgtctttg tgccagtcat tttaggataa 64440tgacatagaa gaaaaattct aaattgacac atcccatccg ctataacaca tgggtactaa 64500tatcaaggat ttacttctga gaaaccctgc acatttaata gagactgtga cttattgata 64560agacaatttg aaaatatgtg cctacagctg ttttatttat tctgccttga taaaaaaatt 64620actcgttaat gtctttattc agtttaaatc tcgttgcttt gtttaacttg tgaaatgcaa 64680ctttgaacaa tacaagtgtg gctttctgtg tttcataagt gtgtgctgta ttagtggtga 64740agaatgaacc aaacttcagg aagatagtcc catatttgtc ttcagaactg gcagatgttg 64800cttgatatat tcctgaagat acaagcttaa aatattacat gatggataaa gaaaggataa 64860agagaaaatc ttgaaatcac gtggcagaag actcctccct caagcctgaa gaagcctttt 64920gtaattttct tgtttctttg acctggactg aattttcatc caatagcact ttttgttttt 64980
cgtgggtagg aatagctctg ctctgtagcc ttccaatcaa ggagcagaag taaattgtca 65040ggtttataga tggtaatgaa taccatgttt caagttgaca taaatcaggt aattccttga 65100gcttcatgtt ccagcttgtc ttacagtata gtttatttaa aaagtatttc aaccttgttt 65160tgatgaactt aaaacatact ttttttatct gataattgtc attttattct ttaaacctcg 65220gcttatctta cagattgtat ctttatgtca ttttctctcc atgtctggtg acaggttgcc 65280tcagttcatg aggctttttg ctactcttct tgcgccgctt cactcctcac ccctgcgttt 65340atctgaaatt ataatgagtt tttcttcttt gctttttagt ttaaaatctt actgcttaga 65400gcttgagaag catctttctt gcgcctcttt gtgttctgta cagtggacta ggaactcttt 65460cttttgattt ctcctctctt tgattaccaa gtctcttggg ctaccagtgg gccttgtctc 65520ccagtttcta tttccagttt gtgatccctg aattctctat ttggtaaggg tctgcctgtg 65580ttttgcatat ttatgataag gaagtatgct agtatgaagc tcctgggcaa agataaagaa 65640taatccagct tattcgggtg ggctccatta ctgcatttca aaatcagaat tttatgtttt 65700gttgattcag gtgcaactag aaatgaaatg ttattttttg ttttggtatt gtgattaatg 65760gagagattca tcctagtttt ctgcagttat tttgggaagt atgtgtgtgt gtgtgtgtgt 65820gcttttaact tgcttgctga aaatttgttt ttccaaaagg acagaattta tccttgatgt 65880tgattttgct tgtagttttg tgttatttag tagcaatcac taacacgtgt tcctgtggtc 65940cttctgtaat actggtatcc cttgttacaa ttgagcttgt gcattatttt gatcaaagta 66000tatggtgtac tattctatgg agatacatgc agtattgatg aaatatggta ttctagccaa 66060gatacatgac tgctttgaga taaaaataaa agcataagca tatatttgtc tgctcttgtt 66120ggacaaacat ttaaaaagtt tagagcacac taaatgctaa aaatgctgtg aataaacatt 66180gaatttttaa gtgggaaatg tacaactggt tttgaatatc cattttacaa agcaagggat 66240atctgtagtt ggacacaata gatgtcaaat acagaagctt taggtcgtat ttttctgtga 66300cactaatgac tgtgatgtat gtctgagagt agagtgttgc ctccattgca tgtgattgta 66360ttgaaatgat tgtaaccaac attatagttt gttcatcgtg gtgcatgttt actgtcagac 66420tatatctaat actaattcat tttgaaatta ggtgggatct caggtccttg gttatggaag 66480atttataaat ataagcaaaa atgtaataag tatgattagt tgaattagtt tctgctaatt 66540tataattctt tattaaaaag cacagtctcc ttttgagttt atccttattg cgtggagaac 66600tctcattttg acttcaacag aggagtaaat tcattttgca aggtgtttgc ttgtattgta 66660
atataaatac tttttctttt gcacagattg ccacactgaa ggtcaaaata ttttattcac 66720tgatggagaa tatattaatc agatagctgc ttcaagagat gtaagtatcc tgaatcttaa 66780gtagctagtg taaatggaat tccttttcct taaattattt agcttttatt agatagactg 66840tagagccttg aacgacctct ttatgtaatg taatgctgtg gatgttttat ttttcttagg 66900atggctttgt tgtcagaata tttgccacaa gcactgaacc tgttctacag caagaattgc 66960aacttaaact ggctagaaaa tgcttacatg cctgtggtat ctcactattc gatctggaaa 67020aggacttgca tattataagt aagatagcaa attaagtgtt ttccctatat tttaattttc 67080aatttttcat actcttaaaa atgaacattg tgttttcacc agttcagctt atgttgtagc 67140agctattttg tgtctgttac tattaatata aaggataatt ttgaattaat atgaataaat 67200aactgggaaa cacagtcttt aaacaaagta tgatatttga agaccattct aaggaattga 67260ctaataattt ctcttttttg ctttgaatag agcaaaagaa aataaaattt aatgagaatt 67320atggatggat attggtgaat gtcattagtt tatatgctgc atcatttttc aactatttaa 67380aaatttgaaa acttacgttg taaatgttat tacaacaata tacatagttc tctcgttgaa 67440atttttaaag tttttacttg aatagatttt caaggtaact atagaatgat ctcatagatt 67500gtcaaaatgg taattggcct tacacacgga tcagtaatct ttcacttgga tagagaaata 67560cagataacca cagagtattt cttgggctta gattatttac ttttgtaatg ttgtaaaagt 67620aattaggatc atgagttctc ctgagagttt tagttttggc ttgccatgcc actccaggtt 67680taatagtgac cttccgtaac ttgtagggtt ctttgcaaga tccatctttt ttgactgtta 67740tctcattaca tttcataaac ttggcattag tgctttactt tctatattct aattatgtag 67800tatttttata attcttccct agaaaaatct tgaattgaaa ttacagattt aaaattttta 67860aagcccataa actagttagt agtttgtata gctggacaaa gtcataaaat gtaccatatg 67920taaagctaca attactcata gttttacaat ttgaaaatat gcataaggat ctgtgtgtgt 67980atatgcatat ttacatatat atatatttga attgatggag tagctatata agatgtcttt 68040ctgtagaaaa agacataggc agagaatctg aaatacttta attcaaatat ctgcatggca 68100ttgttccaag cttgaatgac tctgtgtctg gactaacctc tccaaccttt atatatgctt 68160atttgtaaac caagtggtat agttgtgaga atcaaatgag ataaattttg tgaattgtat 68220aatgcctggt acctaataaa cgctcaataa aactttgagc atttatacat taattcaagg 68280aatattgctg acattgaatt tttaaatttt tttgtcgtat atttaaaaaa ttaatttggt 68340
actggtttgg aacaagagaa acagattatg ataactttat ctttccttgg agtactatac 68400ctatggttaa tatttttaaa aatttgagca catgacattt tgtcatcctt gttttttagt 68460gattttgaat tgacattgca atcacttaaa atagtgcatg ttgtatttag aattcttcat 68520attagaaatg caatagtatt tattagtctc gtatttcagg tacaggattt gatgaggagt 68580cagcaattct tggtgcagga cgagagtttg cgctaatgaa aacagcaaat ggaaaggtaa 68640attattctct ttggattaaa aatgagcatt ctctgattta agagattaaa atacaatctg 68700ttgattagtt tatatagttt gcatgtttaa gaagaatcat cttttaaatt ttgatttaaa 68760attatttttt ataagataaa attttattca tgtagggaac agtacaggca tgcctcattt 68820tattgtgctt agttttgtta cacttcacaa ataattgctc tttttacaaa ttgaaggttt 68880gtgacaaccc tgagttgagc aagtctgttg gcatcatttt tctaaccgcc tgtgctcact 68940ttgtgtctct gtgtcacact ttggtaattc tcgaaatatt tcagacttct ttattattat 69000tatatctgtt atggtgatct gttatctttg atgttactat tgtaattatt ttagggtgct 69060gtgaattgtg cctatagaag acaggaaact taatggataa aagatatgaa aaagctatta 69120tgaactgtgt cttcttggca aaatatgtgt atttcttgct caactcccag aagctgaata 69180gtgtaaaaaa gagtttgttt ttatgtgaat tttatttttc aataaaatat tgatgtcctt 69240ttttttcctc atgtgaaacc tgatgcacta ttccaaaaag taccataatt cctttttttt 69300tttaatacat aagatatatt acactggcaa ataccagagt cttggaatca aacaaggtgg 69360tccttcagca ggaaaatggg ttgagctacc aattacaaaa tctccaaaga tagtacactt 69420ctcagttgga cacgatggct ctcacgccct tttagttgca gaagatggga gcatattctt 69480tacaggatct gctagtaaag gagaagatgg agaatcaagt aagttgagtg atcaactatg 69540catttaataa aaataaatgc tttattttag taaaaataaa actttattaa gataaagttt 69600atgaatttca ataacaccac accaacttta tatatatatc attgacttga tgtaactgct 69660taataaatac atattgaatg aatgaggaac ttattttact ttccaggatc taacaagaat 69720acatgtgaat tacaacttgg gaaaaaaagg agattttaga tatgtgttag tagaatggta 69780ttaataatgc aatagctaat ttagagattg ggccctccag gagtttggta gaaaactaat 69840attactcact gagaatttga atgtacttca gggaatgagg atctagcaat gatagcgtaa 69900atattaattg accagatgag gctagcaggt tcactgattt taaatgacat gttgggaagc 69960ccaggccttt gtgaaaataa acaaatagtg tcataaatgc aaataatatg ggtttttttt 70020
ttagctttat atacaaacca ttattattat tatttgtaca tagaggtctt gatttaatct 70080aatttctttt ttaaagctaa gagcagacgg caatccaaac cttataaacc taaaaagata 70140attaagatgg aaggaaagat tgtggtatat acagcctgca ataatggaag tagttctgtt 70200atttctaaag atggagaact ctacatgttt ggaaaagatg ccatttactc tgatagttca 70260agtaagttaa taaaaagttt tacttttatg caaagaagtt tcaatactag ttatgtatga 70320caaagacata tctagaaaat ttatgttggt ggcatgcttt ctctacattt tttacatttc 70380cttccaaatg tgaaatattt cgaaagagaa attttctgtc atatcttgtc actaggagca 70440ctgtccctag tttcatgcaa agctaatcat agagcttggc aagggaagct tcgctgtagc 70500tgtgacttct gtggatgttt tccctagact caggtgctta tgggacatgt ccctgggaca 70560gtggtagtat cctgaagaag tgattttcat gacatagcta gtttataatt gttgaagtat 70620tttatctccc ccattagggt gtttggctgg gatgatctgt cttatttact attctatccc 70680cagtgcccag cacagtgtct ggcacattgt agggtctcaa gaattatttt ctagtgcctg 70740ggtgactgat aaaccaaaag tttgagaagt ggatatgagg ggtagaacta gctaaatggt 70800tttatattta taaatgctaa tctcaaaaac ttgtcatatt tggttactag attttctttc 70860tagcagctag aaatgaatat aatgagtgaa gcatcccact tggaaggatt ttatgtgtga 70920taaatatgta aagcctttga gtttctttgt aaaaaaggaa actagaatta atgaagttta 70980tgattagaaa tatatgtaat tgtcccatgc tggggaaatt acacctgtag aacatttata 71040gcaattaatt attaagtgga ggtgtaccca gatgataagt gaactggctt aatgttgtaa 71100tatttagatt ctcaattggc tgcagctatc agagtaaatc aaaatttggg agtgggttct 71160tgtacagttt aaaaataata ttcaatataa ttgttgcttg ctaacatcca ctgggtagag 71220ctgatggaaa cttgtatatt tcttcaacat atatttattg agcatgggtg atacattagt 71280gattgatgga cctgacagac ctggatactg cctttttgag gatccgagag acagttgggt 71340attacagaac ttaaatattg gggaagtggt attgaatggt tatggacata gactgagatc 71400ctagcaccaa cacttttttt ttttgagagg gagtctcact ctgtctccca ggctggagtg 71460cagtggcaca atctcagctt actgcaacct ctgcctccta ggttccagcg attgtcccac 71520ctcagcctcc caagtagctg agattatagg cacgtgccac aacacccagc taattttttg 71580tattttttgt atttttagta gagacgaggt ttcactgtgt tggccaggct ggtctcgaac 71640tcctgacctc aagtgataca ctcgcctcag cctcctcaag tgctgggaat acaggcgtga 71700
gccaccgcgc atggcccttg gcaccaacgc ttttaattgg gtaacagttt acttaatctt 71760taactgggta acagtttact aaacatttaa gagttttctt tttgtttttt tctacctgca 71820aaaaatagat aatgatagca cctgcttcat atagttgtta tgaagattat aggaggtaga 71880acttaagaaa tgcttagcac atcatgtaag gtctcaaagc atgtcagcta gtactgttaa 71940taataataat aaataactac tgttttatat atatacacac acccttacct ttttcaaatg 72000tgctaaatat taaaagtgga aatttttgag gtataattaa caaaaagacc taaactagtg 72060aaaggtgaaa gtgaaggcca ccttaaggta gtgatatctg agctaataat gtagaacagt 72120tttcccaaag cataatatga gattgatact atttgtaatt taaataaaac aaatacttga 72180taacatgttt ttattttaat gtatattgga aaaataataa tctttgattg ttaatgttaa 72240agatgcttca atcaatttag agttgataca aaacatatta aatagataat atgagtggca 72300cttggatatg acaaatataa tgaaagtaat acatgaataa gtgaagttta gaaaataact 72360gtgtattgga ttgtggaaga aagcattgta ggttgaaaag gattaaaaaa tactgttttg 72420aaaagtctca ctggtttcta cataaacaat ttcatgtcaa tatactatgg caagtatttt 72480actttgtttt gctgaggcag gtcaaagcag gtaaaataaa ttatttatgg actcctttgt 72540gcttagacca gaaaagtttg aaagtactgt ggagaggtat taccatttgg aggaagcatt 72600caggagaagt aagcttgaga gaaagctaca gtaaagatat gtcccaagtc tggatgataa 72660tgttttagga ttcactgaag ctacgttaca aagttacaaa gttaattcag tgatactctg 72720ccagtctttt ggctacttat ttctcatttt gttgagttat tttgccccag aaataaacgg 72780ttctgcattg aggtagttct tttactaggc tcagaagggc ctttttgttg tttttactaa 72840atttgtttct ttcctatagg tttggtaact gatttgaagg gccattttgt aactcaggta 72900gctatgggca aagctcacac ttgtgtttta atgaagaatg gagaggtgtg gacatttggt 72960gtaaataata aaggacagtg tggacgagat actggtgcca tgaaccaagg tgggaaaggt 73020aggtctttaa ttcttagata ttaaaatttg tgttgtcttt tcattagttt ttcagaacaa 73080catagtgaaa agtcttgttc ttttcaaaat gagtggctct tctttttctt tttgttccta 73140tttaaatttt ttttaaaaat tttatgggta gatagtaggt atatgtatgg ggtacatgag 73200ttgttttgat acaggcctac aatgtgtaat aataatcaca gggtaaatgg ggtctccatc 73260atctcaagca tgtatccttt ctttgtgtta tgagcactcc agttatactc cctcagttgt 73320taaagtatac aaaaaattat cgccaactgt agccaccctg ttgtgctatc aaatagtaga 73380
tctcttgtat ctaattatat ttttgtgcca actaaccatc ccatttccca cccactctcc 73440ctgactaccc ttcccagtct ctagtaacca gcattgtact ctctatcttc atgagttcaa 73500ttgttttaat ttttagctcc cacaaatgag tgacaacatg tgaagttggt ctttcggtgc 73560ctggcttctt tcacttaaca taatatcctc cagttacatc cttgttgttg caaatgatag 73620gatctcagtg ttttttatgg ttaaatagta ctaccttgtg tttatatgcc acattttctt 73680tatccactca tctgttgatg gacacttagg ttgcttccaa atcttggcta ttgtgaatag 73740tgctgcaata aacatgagag tgcagatatc tctttgatgt actaatttcc tttcttttgg 73800gtatatccat agcagtggga ttgttggatc atattgagtt ctagtttcag ttttttgggg 73860aagctccata ctgttctctc cagagtggct gtactaaatt tacattctca ctaacagtgt 73920acaagtgttc ccttttctcc acatcctctc caacatttgt tattgcctgt cttttggata 73980aaagccacgt taactgggga gagatggtat ctcagtgtag ttttgatttg catttctctg 74040atgatccatg atgttgagca ccttttcata tacctgtttg ctattcatat gtctactttt 74100gaaaaatgtc tattcagaac tttttcacat tttttaattg gatttgagtg gctctttgac 74160gttatatttg tgaattagga aaaccactga atttcttatt tatttgataa tatttactga 74220taaaatatca ctggaaaaac aaatacactt tagatttttt atttttgagc aaatgtgctg 74280gcttactcca atcatcttct aaattctaca taaaatagtg cttctttggc ttagcatctt 74340tgatgactgc attactccag aaaagtcttt tccaaacatt tttttattag ctgctgttac 74400acgacatgtt ttaaatttta accatttttg gcagcaatct ttctggcgta tattatcttc 74460ccatttaaca gcacatgcta cctgtaattt aacctgttct tgacaactaa gagtgatcct 74520tatgaatatc tgacacttta ccaagaaaag actaaattgt agacacacct tcttcacaat 74580atcctttgct ccaaccctca ggccattgaa gagaacaaag ggctttctcc tttgattgcc 74640ctgtttgctg tggtcttgtt ttgttgttgt ggttgtaaat agcatgtcta cttcttgccc 74700tcaattttta tatctagctg ctatcgttat gtttataact ttcactccaa tttctgattt 74760tggtcaagag attaaaccat acatatatat tattagaact aagcgttgaa ctttggttaa 74820agtaccaata atttcgagga ttaatgctgt ataaattaac aaaacctggt ttttctgttt 74880tgaggctttg atgtcagact catctggatc tcagaaaccc accattcttt gtctttgcct 74940tattcgtgtg tctgtgtgtg tatttaaaat gcatcacttt aaaaatgagc aagttactag 75000aatatattgg caaatgaaga aggaacaaaa gaaagaaaag aaatggatta cttcacaatg 75060
gaaatacttt gatttattac taaattagtg aacttgtttt ggataataac tgctaggaca 75120aaggggagaa tcgggttttt ttaatatata atatgaatat atattcagat tatgttttat 75180aagaagtctg aactaaacaa cattgtttcc ttattctgaa actcttgtga aaattgagcg 75240tacatttaca cctatgttct agattcttca gtgacatttt gtaataatca aagtaaaatt 75300gttaaaagat ttttttaata agaatcatac ctaccctgct ggtaagtaat gaattagaaa 75360gcagatgagt aaagtaagac acatacagtg catactgatt gcagagtcat aacagatgct 75420ggggagaatg tggagaaagg ggaatgctca tacttggcta gtggaaatgt aaattagtac 75480agcaactatg gaaaacatag atttttcatt cgtatgaagg tgactcaaaa aactgaaaat 75540ggagctgcca catgatctag caattccact tctgggtata tgtatcaaaa agaaaggaaa 75600tctgtatgtc agagagatgc ctgcattctc aggtttatca tagccatatc cacaacacca 75660agatatggaa tcaacttaag tatccatcaa cagatgaatg agtaaagaaa atgtggtaca 75720tacacaatag aatattattc atccctaaaa aagaatgaaa tattgttatt tgcagaaaca 75780tggatagaac tgaaggacat gatgttaact gcaataaaat aaaccaggga cagagacaaa 75840tatcactcat atatgggaac taaaaaattg atttcatgga gatagtaaat agaatagtga 75900ttaccagaga cttgtaagga tagtgggagg gggagaggaa gagtggttgg ttaataggta 75960caaaaataca gttagagaga aggaataagt tctaatgttc aggagcagag tagggtgaat 76020gtcgttaaca acaatatatt gtgtatttca aaatagctag aagagagaat ttagaatatc 76080ccagttatct tgacttgatc ccagttaccc agatttgatc attagacatt atatgcatgt 76140atgaaaactc acatatatgc cataaatagg tataacagtt atgtatcaat ttaaaacgaa 76200cgtaaaggaa tattttaaaa gtttatttaa accaggaaag tacagaacat attgaaatat 76260taaaaaattg tgtctctacc atgtcttgtc attttttttc agttaaaaaa aaatcacaga 76320taagattcaa gtgccccata tcccccttcc aaggcttatt atcatattcc atttctcccc 76380agagctaacc gcgatcatga ctttgataag catacgttca atcatgttct tatatatttt 76440tacatgttct tttatatttt atgtgtaaaa taatattgca taatgatgtc tgttttacac 76500ataaaatata ctactgtttt atgtgttttt ataagtgtta atctgttatt tacatttttc 76560tacaaatttt tttttctcat cattctgtta gaaatatgtc catgttggta catataaatc 76620taaatgattc atgttaacta atgtgtaata taccacacag tgaacatgct atagtgtatc 76680cattctcctg ttgatggatg ggcttatttc caatttttca ttgttataaa tgagtttaaa 76740
acctccttta taaatgtttc ctggaaaacc tgtgagactt tctctgatgt aattgctcta 76800ttgtggattt cagttttact ggatttttct tgaaagagat tgtaacaatt tatactctca 76860ccagtagagt tggattttcc acatcacatc tgatattgac agactgattt ttgtcagttt 76920ggtggctatg aaatgatact gtaatttttt atttgttaca ttgaagtata atatgcatgc 76980aggaaaatat acatagtatc ttgatgaatt ttctcaaatt gattgtattc atgtaatcag 77040cacccagatt aagaaaccac atattaccag cagcccagaa agccccactg tatgttcctc 77100tctgcccttc agttaattac cataaattag ttttcttttt atacttgata taagtgaaat 77160catgcagtat gaactctttt gtgtccagtt tctttttatt gttttatgtt tatgaagttc 77220atacatattg ttacaagtag ttatagattg ttcatttttg ttctattaaa ttatatatag 77280atactacagt tttaaaattg tttctagtat ttgggcattt ggatggtttc cagttttggt 77340tattatgaat aatgttgctg tgaacattct agtaaatgtc ttttggtgaa agtgtttatt 77400catggctttt gggtatatac ctaggagtag aattgctggt tctctcactt ttcttttaat 77460tttcattccc ttgattatta atactgagtt tgaacatctc ttcaaacttt attttactta 77520ctaaaaattg atttgtagga gcttttttac gtattatgtt ttatatatct ttgactaacc 77580tgtggtttac agtttcattt gttatgtata attttaaaat tttgatgtca aaattttcaa 77640cctttttctt tataaatttt tgatgaatgt agcctggtga gagattttat tttccatata 77700aattagaacc agctttattt gcttggcatt gtgattgaga ttccattgac tgtaaagatt 77760aatttgagga taatagttcc agtattggaa tgattaaatg agtaatgctt atagagctag 77820ttacaaatag tgctcagtac attttagcta tgattattga aaatagtcac tgaggcttcc 77880ttcaggatca ctttattcta atcctagata tagctagctg atgcttggtt atctaatttt 77940tctgcttgcc catagattag aagtggcaaa accccaaaag gctatatatg ctatgagttt 78000atttcttggt tagatagacc tgtattgagt gatagctgta tgttaaatgg atgtgatagg 78060tgtgagagaa taagattgac taagaataag agatattatg agcaagactt aacctcataa 78120cttagatcct ttaggaaagt caaatataaa aaataattac ttgactccca cagatttgtt 78180ctcctgtcat agggtcttat aacactgtgt tccttcaaaa ccctaccagt tgtagtttac 78240atttttattt ggttatttga ttaatgtctg tctttcttac tacattataa gcttcataac 78300agctcagtat taccgtcttc agtttctaga gcagaggtct gcagagtata acctgatgtc 78360atttttttat atagccctct aagctaaaaa ttgattttac attgtaagga ttgtaaaaaa 78420
tgtgacaaag atttgtatgg gccacagagc ctaaattatt tactggctct ttataaaagg 78480tttgctgatt tcttgccgtg aaggaatccc tggagtatag taggtgttca atttagaaaa 78540aaagatgaat gaattgtatt gtaacaccat tttaaaaagt tgttaggtga ggtataaaca 78600ggcactgagg aggtagtgac tgactgcctg gaaaaggtag caagtgcttt aatagggaat 78660gacttctgag ctgaattttt gaaggctgaa gtttctctac tagatgagat aggcagagac 78720atcttgtaaa tgaggaagga agaggcagag ggagcaagaa tgaacaaagg ccctgagcat 78780atggaaggac acagggtgtt tggagagcag agggaaaaga aatgagattg gagaaaatta 78840ggagccatct tgtgcctact gcctcttgct gagttttgtt cttctgaaga ccttggaagg 78900tcactggagg tttgtaaatt atgaagttgt catgtgcttt gggagggtga ttggtggcag 78960taggaggatg atctagagtg gggaactctt ggcttggagc atggttggga ggctttcttt 79020ggtctgttga atgaaagtgt tggtagtgag gatggaaaac aggaaaagat ttaaaagtat 79080gattgacaga aggtaacagc caagagacat ttgacaatag ccttccttgc cttcttttgc 79140agaaatgaaa tggggagagt gtttattagt ggtccctaaa ttaaaattta tttttgttac 79200tcaaatttaa gcatttcttt gtataattgc ttggattagc atgctgtcat tttttaaact 79260gggaaatata gtcatccatc ttttatacat gaaagtaacc tgtataaaaa ttacaaattg 79320atctgtcaaa tagggtttgg agttgaaaat atggcaacag caatggatga agacctggaa 79380gaagaactag atgaaaaaga tgagaagtct atgatgtgcc ctccaggcat gcacaaatgg 79440aagctggagc agtgcatggt ttgcactgtc tgtggagact gtacaggtta tggagccagc 79500tgtgtcagta gtggacggcc agacagagtc cccggagggt aagagacaat gattcctact 79560aaagaacatg tgcagaacac atttctttgc aaaatcattc cggagtatac tctactatta 79620atattttttt cttaaagacc agtggcatct tcacttgatc ttagccaaaa ggccaagaaa 79680gtattataat tcttataatt gttttctgtt ataataattt ataatttaat gtcatctcta 79740aggttgcctt tctttcattt gattcattta aaccattatt taaaaacaga ccataagttt 79800ttcaagactt ggggctgtac ttttatttgt attatttttc aaagcctatt taatgctaga 79860tatctattat atttctttct gttgaacatg aatcttttct aaataaaatt atcgatatca 79920taaagagtac ttgattagat atatagtttt gttttaaatt aaatgtacca tttataaatt 79980tagaaacgtt gtatgcttgc ttctaaagtt acatcatacc tgctatattt atccttgttc 80040tcaatggacc acatttttca gggttctata ttattgtcaa tttacagtta atatgtaagt 80100
ttggcagaat aagaatgcag gttttctgct aggatgttag gaagaatttt gaatctattt80160tctacattgc tagttatagc cactataaca tgatgggata ttctttgtgt gatcagcttt80220gtaataattg gctgatttgg gggagtagag aaaattttgt gaagagaagc ccagaaggaa80280gacagccatc cctgcctctt gaccttcctc tttgtcaact ctgtaatagt tgcttcttcc80340caaatattgt ctggatctaa taggagttta tttctaagtt tatatttggc ctttcatagt80400taaataggtc atatataaca ggccttttct ttaaggctgc tgagatatga tttttttttt80460ttttttgaga cggagtctcg ctctgtcgcc caggctggag tgcagtggcg ggatctcggc80520tcactgcaag ctccgcctcc cgggttcacg ccattctcct gcctcgacct cccaagtagc80580tgggactaca ggcgcccgcc actacgcccg gctaattttt tgtattttta gtagagacgg80640ggtttcaccg ttttagccgg gatggtctcg atctcctgac ctcgtgatcc gcccgcctcg80700gcctcccaaa gtgctgggat tacaggcgtg agccaccgcg cccggccatg aacttttaaa80760aacaaatttt ctttacatta ttagtataag caataaagtc gtctttatca aaggataata80820tttattctgt agtgctttat gtttggctct gttttctgtt ttttaagcac ctaacttctt80880tatttttaaa tttaatttgg atgcagaaag actctgatta ttttttaaca atgttggaat80940tgaaatttat tctcctggat ctctccaacg ctatttccat taaaattcag tgttagctgg81000gtgtagtggc ccatgtctgt aatcccagca ctttgggagg ctgaggccag aggatcactt81060gaggccagga gtttgaaacc agcttcagca gcatagcaac acccctaact caaaaaatat81120ataataaaaa taaaatttaa tatccccact ctctgagctt aagcagccag tatatatcgt81180gaacaaaatc tataaattat ctggtccctt ttgaaattaa ttattcttcg ttttttcctc81240cagagactta cataatttat aataattatt catagactta catggacaat actaaccaag81300tataataatt gctttatgaa tgaagcaaac tgaatatatc agaatattaa ggactattaa81360gctgacttca attattataa aattgtgact acatatctta ttatgataaa gtacatataa81420aattgaccag cttaaccatt tttaagtaga cagttctgtg gtattaaatc cattcataat81480gttgtgcaac tattaccacc atgcatctcc ataacttttc atcttgtaaa actgagactc81540tgtaactgtt aaacaatgac tcctcattct tccctctccc ctgcctctgg catccactat81600tcttgttttc tttctgtgtc tgattgacta ctctaggtac ctctcatata aagagaatca81660gagtatttgt cttttttgtg actggcttat ttcacttagc ataatatcct cagttcatcc81720aggttgtcac atatgtcgga acatctttgc tttttgaggc tgaataatat tctattgtat81780
gtatattacc acattttgct tatccattca ctcactgatg aacactttgg ttgcttccac81840attgtaacta ttgtgagtaa tgcgctgtga agatgagtgt acaaggagct ctttgagacc81900ctgttttcag ttcttttggg catataccca gaagtggaat tgttggacta tatggtaatt81960ttatttttaa tcttttgagg aactgccata ctatttttca cagcggccaa ccattttaca82020ttcccaccaa tagtgtacag atgttccagt ttctccacat ccttcttaac acttatgatc82080tgttattttg gtagtggcat cctaatgggt gtgagatagt atctcattat agttttgatt82140tgaatttctc cagtgattag tgatgttcag catctttcca tatacttttt ggccatttgt82200agatctttgg agaaatgcta ttcaagtcct ttgctcactt ttgaatcagg ttgtcagttt82260cttttgttgt tgttgatgag ttttaggaat tctctatata gtttgaatat taattcctta82320tcagatatat gatttgaaaa taaaaagttc tgtctgtggg ttgtgttttt actctgttga82380tgttgtcttt tcaagcagaa aatttttaaa attttcatga agtccagttg tctattgttt82440tttggttatt gtcgcctgtg cctttggtat catatccaag aaattatttc ccaatctagt82500gttgtgaagg gttactgtta tcttttcttc ttcttttttt ttttttgatt atactttaag82560ttttagggta catgtgcgca acgtgcaggt tagttacata tgtatacatg tgccatgttg82620gtgtgctgca cccattaact cgttatttaa cattaggtat atctcctaat gctatccctc82680cccgcttccc ccaccccaca acaggccccg gtgtgtgatg ttccttttct tctaagagtt82740ttttgttttt ttttgtaata gttttaggtc ttacatttag gtctttgatt cattttgagt82800taatttttgt atatgctgct agagaagtgt ccagatcaag ttttcccagc aatgtttatt82860gaaatgatta tcctttctct attgaaccat cttggctcct ttgtcaaaaa tcatctgacc82920atatatgtga aggaagattt ctcagctgtc tattctatcc cattggtctg tatgactgtc82980tttatgccac accacattgt tttgattact gtagatttgt agtaagtttt gaagtcaaaa83040ccgagtactt tgttcttctt tttcaagatt gtttggctat ttgaggtccc tagagatttt83100ctatgaattt taggataggt ttttctattt tgtaaaaacc atcattggga ttttgatagg83160gattacaatg aatctgtaga ttgctttggg tagtatgaac atcttaaaaa cattaagtct83220tctaatccat gaacatagat gtgtttctac ttatgtcatc tttaatttct ttcagcaatg83280ttttgtagtt ctcatttcac ctcattggtt aattactaag tgtattcttt ttgatgctat83340ggaattgttt ctgtaatttc cttttcatat tgttcattgt tagtgtctag aaatacaact83400gatttttgtg tgttgacttt gtatcttcct actttgctga attcattttc tctattggtt83460
tttttgagtg caatctttag ggttttctac ttatgagatt gtattatctg tgaacataga83520taattttact tcttcctttc tgctttggat gacctttatt tcttttcctg tccaattgcc83580ctggctagaa cttctagtgc tgtttaggat tggtcaaagt gagcatcctt gtcttcttcc83640tgatattacg aggaaaagct ttcagtctct caccattatg atgttcactg tgtgtttttc83700atatgtggtt tttatgttga gatcatttcc ttctattcct agtttgtcta ctgtttttat83760tatgaaaggg cattgaattt tgtcaaatgc tttttgtaca tcaattgaga tgatcatgtg83820tttcttttct ttcattctgt taatgtgtta cattacatgg attgattttc atttgttgca83880ttccaggagt aaatcccact tcgttatggt gtataattct tttaatatcc tgctgaattt83940gttttgttag tattttgttg aggattttca catcattatt cataagggat attggtctgt84000aggggttttt ttttttgcct ccccctgccc ctctccctcc tccctttctt ctctttctcc84060ttctccttct tctttcttct cctcctcctc ttcctccttc tccttctttt cctccttctc84120cttctcctcc tgctcctcct cctcctcctt ctccttcttt tcctccttct cctccttctc84180cttcttttcc ttcttccttc ttcttctttc ttctcttctt tcttcttctt tttcttcatc84240ttttgtctga cattggtatt ggggtaatcc tgacctcaaa gaatgaattc ttgttctctc84300ctcttcagtt ttttggaaaa ttttgagaat tggtgtcata aacactattt taaaaatagt84360gtcagtcagg catattactg tgtcacagtt tttactgtgt agtaagtgat cttccaaatt84420gtttataatg cttgctttta aaagtgacac atttcacagg gtgtctagct atgtctttta84480aactgctgcc agatatgttc ttccttttgt accttctgaa atctgtataa cctggggttc84540aacaaggctg tcaaaagttt caagaactga tctgatgaac tacattaaag ctataccttc84600tacaaaatat gtgactaaga agttactttc cctgacccca agtcagtttc tgggagtgtc84660tttgtcaaca tataccccat atggttcaca atcgatatga aagggctgaa ggagaaaaag84720aacaggtaat tggactctgt gaaaggagag aatgggaggt gaaggcatgt aaagaagaga84780gggggaagga gacatatatg tgtttgtgtg tgtgtatata tatgtgtgtg tgtgtgtata84840catatatata tacatatata tgtattattt tttcctttcc aatgtgtgaa tgtttttatt84900tcctttgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgttacttt acgttctggg84960atacatgtgc agaacgtgca ggtttgttac ataggtatac acatgccatg gtggtttgct85020gtacctgtca accggtcatt aggtatttct cctaatgcta tccctcccct agcccccacc85080ccccaacagg ccccagtatg tgatgttccc tttcctgtgt ccatgtgttc tcattgttca85140
actcccactt atgagtgaga acatgcagtg tttggttttc tgttcctggg ttagtttgct85200gagaatgatg gtttccagcc ttcattcatg tccctgcaaa gaacacgaac tcattctttt85260ttatggctgc atagtattcc atagtgaata tgtgccacat tttctttatc caacctatca85320ttgatgagca tttgggttgg ttccaagtct ttgctattgt gaataatgat gaaataaacg85380tacatgtaca tgtgtctttg tagtaggatg atttataatc ctctgggtat atacctagta85440atgggactgc tgggtcaaat ggtatttctg attctagatc cttgaggaat taccacactg85500ccttccacaa tggttgaact aatttacact cccaccaaca gtgtaaaagc gttcctattt85560ctccacatcc tctccagaat ctgttgtgtc ctgacttttt aatgatcacc attgtaactg85620gcgtgacatg ttttctcatt gtggttttga tttgcatttc tctaatgacc agtgatggtg85680agcttttttt cgtttgttgg ctgcataaat gtcttcgttt gagaagtgtc tgttcttatc85740cttcgcccac tttttgatgg ggtggtttgt tttcttgtaa atttaagttc tgctgcataa85800ataaatgtct tcttgtaaga agtgtctgtt catatccttt gcccactttt tgatggggtt85860gtttgttttc ttgtaaatgt gtttaagttc tttgtagatt ctggatatta gccctttgtc85920agatgagtaa attgcaaaaa ttttctccca ttctgtaggt tgcctgttca ctctgatgat85980actttctttt gctgtgcaga agctctttag tttaattaga tcccatttgt caattttggc86040ttttgttgcc attgcttttg gtgttttagt catgaagtct ttgcccatgc ctgtgtccta86100aatggtatta cctaggtttt cttccagggt ttttatagtc ttaggtctta tgtttaaatc86160tttaatccat cttgaattaa tttttgtata aggtgtaagg aaggggtcca gtttcagttt86220tctgcatatg tctagccagt tttcccagca ccatttatta aatagggaat gggaaggaga86280catattacat gaagctggca agcttaggct ctgttgctga aggctaccaa cacctgcctt86340cacttatctg ctggacagca acattgtttg attgttttta gccaaccaac ccatctgggt86400cacttaatcc taaagcttct ggtgcttcac taactgcaaa aagactttta aaggattgtt86460tttaaagtag tgactttgat gatagtcgaa tctgtgtgta ttttctgtag aaatatacca86520attatgccta ttgggagttt aatcagtctt gtttttaatt attctgctat ttatatcttt86580tctttaaaat gaacaatcgt gatctaaaga ggtgatttca ttgcttactc atttaacaat86640ttcaaaaatg tggaaaataa gttatagaaa agagttttaa agacaaatta ggaatatctc86700agttgtcttt tttttttttt tttgtatttc cccctaggat ctgtggttgt ggttccggag86760aatctggttg tgctgtgtgt ggatgttgca aggcctgtgc aagagagtta gatggtcaag86820
aggcaagaca aagaggaatt cttgatgcag tgaaagaaat gataccttta gatcttcttt86880taggtaattt tgattgatta tactatgcta cactgagttg tcctcaactc agtaagtctg86940acagtttaaa caatttcttt tagatatatg ttaataaatt aggataataa ttaatgtatg87000caatactgct tttacggtaa ctgacaatat gacattgttt aagggatgag attctttttt87060ttttttttct ttttttgaga tggagtcttg ctctgttgcc aaggctggag tacagtagct87120caatctcagc tcactgcaac ctccgccttc taggttcaag tgattgtccc atctcggcct87180cccgagtagc tggaattaca ggtgcccgct accacacctg gctaattttt gtatttttag87240tagagacggg gtttcaccat gttggccagg ctggtcttga actcctgacc tcaggtgatc87300tgcccacctt ggcctcccaa agtgctggga ttacaggtgt gggccaccat gcctagccag87360aatgagattc tttttccctt tttggctaag aattattaaa gtaaaaatat tgtgtgtttt87420agacaatgtt tttgctagct tttactttct tgaattttat gtagttctgt catagctatt87480atataacata tatgttgttg ttgttgttgt ttttggccaa tcatctagct gtcccagtgc87540ccggggttaa cattgaagaa caccttcagt tacgacaaga agaaaaacgg caacgtgtaa87600tcagaaggca cagattagag gaaggaagag gtaagatgta gctacagaga aaaagtacat87660gaaaatccac tttcctaccc tatcctgaaa ccactgaaaa gttcacacaa taaactaatt87720tggtaagaaa tcatcaaaat taaaattagc aatattctga taactcatat aatgggaaaa87780tattcagggt attcatgctt cttctaataa catacgttta ggtatttata attgtgctaa87840taacatactt ttaattcagc agggtgttta tggcataagt cagccatatt aagaactgtg87900ccatctgcct actatcaata actattagaa atggcaactt tttaaaaata gatattctca87960caacttgtcc ttcacccgga ctttggactc atctatttga ccgtggaaca aaattcaatt88020tagtcttttt catttctatt ttcttactca tttatgctca ttatcatttg gacatagtcc88080tgctatgctg ccctccagtt cagcacataa ctatgctttc atgtgtcctt ttttggaaaa88140acctcattct agttatttac aggaagagta agatggcagg tctgcaggtt tcatttgcaa88200agataggtct attgtttatc ttttcctcat tgtttatgag cttaaaagtt tcttagctta88260aaaatacatt taactggcca ggcacggtgg ctcacgcctg taatcccagc actttgggaa88320gctgaggcag gcagatcaca agatcaagag atcaagacca tcctggccaa catggtgaaa88380ccccatcact actaaaagta caaaaaaatt agctgggcgt ggtggtgtgc gcctgtagtc88440ccagctactc aggagactga ggcaggagaa tcacttgaac ctggcaggtg gagcttgcag88500
tgagccatga tcgcaccact gcactccagc ctggcgacag agtgagactc catctcaaaa88560gaaaacaaac aaaaaaaatt taactgtggt tataattggt atttgaatac gcaaagataa88620atatatgtat tactaaagat ttccttgctc agtaaatttt ttttttgtag gattaagaca88680aattgttatg gaagtctatg attttattcc tccaataggc aaaattttga ttagaaatgt88740ttactgatat tgcatatttt aaatgcttat tagaaatggc atgatatttg atgcatgaaa88800taaacctttt acccatttaa ccatcattat atttttttct agctattctt tttgttaata88860tatggaataa gtagtaaaat gtataataca catggtgaat tctagtttta ctaaacttaa88920attgtaaaac atatacttgg cacatgaaga gtgttcttca tagcatctga taagtgatgt88980tgattgataa ggtgaatatt tactattgtt tggctttgct tgcataatgt cctttaaagg89040atattttaat gagattaata tagatactgt cagtatttac attatataag tatatgggaa89100aattgcatgt taaatatttc ttacaaaaca ggatagaatt aagtttctga tgtggtaatt89160aataattttt tatgttgata taattttttc agattagtta ccattctatc tgtttgctac89220ctgtttcttc tttctttaga aatattaggt tgagcgacat gaatgaacct acaaaatgca89280gctatttctt acatttaaac tacagtatag cacatcctta cacttaatta ttcacattta89340attacttgcc taatgcggtg tatcttgtta ctctttttct cttccctcta aaaaagctat89400tcttctctag gtatagtata tttgtgcata taggaaataa aggaacattg aatcgtgcat89460tgataacttg acataacttg gtgattgcag aatattcttt tgccatttat ttttaaattt89520gacataaacc tgtgaaacat aattgtaaac attaaccctt taaagttaaa aaaaaaatcc89580tccactagct aaatatttaa agaaattatg ttgttgtatc tgactttcca aagatagatt89640gaggttctag gcacttagtg tatattttgt gacaaatagg tttcatctgc agtcctaaat89700atatagaaca tcagtagaaa cactttgttc agattattgg tgtacataaa ttttatcttg89760tccttatttt ttggtagaac tctttcttag atcaccctcc ttcatgtttg gtaaaacaat89820atatagtgaa tatcttttct tgagtggaat cttcagtatg aacctcaatt atcacagtcc89880ttgctttcca tggcactaaa attaacagaa atgtgtgcac attggaacca tgtcctcccc89940tttcacagtc accacagtta cctagaatca tgcgaaagga ggacagagtt cccgtatttc90000tgtgtttcag ttaacacagc actatgcaaa gtgatgcctg cttgtatgtt ttgtttgttt90060gtttatttat ttatttattt tgagatgaag tctcactctg ttgcccaggc tggagtgcag90120ctgcacaatc ttggcccact gcaacctctc cctcccaggt tcaagcgatt ctcctacatc90180
agcctcttga gtagctggga ctacaggtgc ccgccaccac gcctggctaa tttttgtatt90240tttagtagag gcgaggtttc accatgttgg ccaggctggt ttcaaactcc tgacgtcaaa90300tgatccacct gcctcagcct cccaaagtgt tgggattata ggtgtgaggc actgtgccca90360acctatttta ttttaaatga atatttttgc atttggacaa gaattctaac tatgtattca90420ttttttaaaa aatcaaactt cagagtcatt cagtgacttt ggaaatcctt atactgtatt90480aagtaattaa aaatctattc tgatatgaca cttttatgat tttatcttta tattattctt90540agcactccat taagcaattt ttatttaaaa acaaattttt ataagaaaac aaaattagat90600gaatatgata tacttacact tccatagaga taaaacaaga aagataaata ccaaaatatt90660aacaatgctt tttcctgaca ggtgagttta tagatatttt ttattttctt ctttacaatt90720tgtttcctaa atattctcca ataagcatat tagtgtataa atagagtaaa agttatgttt90780tacaactgaa gccattttaa aaaaatactg attaggttta aggcatccat actttgctta90840cctttgcttt ttaaaaaata tttttatttt aattggatgg aggactctct ttcaggtgct90900gtgcttttca ttactttctg gccatggttc tcaaggtggt ctcaaaggga ccagcatcac90960ttgggcactt gttagaagta ccagttctca ggccccactt cagatctcta ctgaaacaaa91020aaactggggg agtacagcca gtaaactgtg ctttaatagc gttccaggtg attctgatgg91080tcccaaagtg tgagacttag tgtgctaggt cagttgtttg cactttgcgg tttgatccaa91140tgaccttctg catcagaatc acctgggcct aatgaatcag aatttatggg tcaagggtct91200tggaaataga tctgagtcac ttgcacagtg ttctaaggct cttctctggc actgtgctag91260aaaagattgt agtggctttg ctcccatgag gaatagaaac atcaaataaa atgttagtgt91320tccggctgcc atacatacag atcttttatt tctttggcct gttgatactt tttagaaact91380aaaaatttag tagtatgtta ataattatac ttactagtaa ttggctttta cctgtgttaa91440catttcagaa tttaagaatt aggttatgtt agattatttt acttgacttt tctgtctcat91500cttatttacc aagtctgaac taagtttttc atagatgatc agaactctgg gctctagttc91560tttttttttg agtcacagtc ccactctgtt acccaggctg gagtgcagtg gtgcgatctc91620ggctcactgc aacctccgcc tctcggtttc aagcgattct cctgcctcgg cctcccgagt91680agctgggatt ataggcatgc accaccacac ctggctaatt ttgcattttt gttagagatg91740gggtttcacc atgttggcca ggctggtctc aaacttctga cctcaggtga tcagcctccc91800aaagtgctgg gagtacaggt gtgagccact gcgctcggcc tgggccctag ttcttgtact91860
aaagccttgc tcagacagct tcaggcctat tgacgtaatt tagttactgt tttatgacac91920caggtctctt ataatggaat gtgttattga aacccaagaa agcaatgata ttactgcttc91980ttcatacatt tggaactttt taattttaaa tctttgtgtt attaatagtc tagttaattt92040ttttaattta tctttttagg tattaatagt attcatttat agattttagt tcaatggttt92100gtgataactt gtctttttta gattatattg ggttgattga tgtattaatt taactgtgca92160agtgaagtgc ttattcagtg aaattcttaa gtagggctct tgaagtcatc agaagaacta92220gtggatgatc atatcaatga ttttatcctt atattccttt tcagcactct gtttcgtaag92280gaaaattaaa cttcaataat gttagctttt tcttctttct tctaagtatc caagtttcat92340aattgtgagc tgtagatggg ctaaacccat gtcctggctt acatgggaga gtcccatttt92400ataggtgtcc aggcatattt cttttttttt tttttttttt ttttttgaga cggagtctcg92460ctctgtcgcc caggctggag tgcagtggtg ctatctcggc tcactgcaag ctccgcctcc92520tgggttcaca ccactcttct gcctcagcct cccgagtagc tgggactgca ggcgttcacc92580accacaccgg gctaattttt tgtattttta ctagagatgg ggtttcactt tgttagccag92640gatagcctca atctcctgac cttgtaatcc gcctgccttg gcctcccaaa gtgctgggat92700tacaggcgtg agccaccgcg tccggcatgt tgctattact ttttaagccc ttaaaaccat92760tatggttttc ttgatttgac gcatcaaatc caatattagc tgatattcca gtgacacata92820aaagtctctt tttaaattaa agtattatat gaagaattta gagagtataa gtaagaaaaa92880atagatcagc ataacccttt atacaaagag aaccattagt agaattttgg tgtaacttct92940cacacttttt tacttatttc atataatttc atactgtgat agatattggt gtcctgtttg93000tattcccttt aatattatat catgaaattt ttttgtgctt tattgcaaat accatattaa93060agtaatatag gaatttaggt caaggttttg cataaaccta ttccttaatt caattattgt93120ttttatttcg ttgtcatctt ctaatcaagt tttacttatt tctagttata tcattgactg93180aattttgttt tacatttttg cttagtgtta taatacatat atttttatgt tttgcttgta93240ttgtacccct accttcccat ttccatcccc ccctcaaagt aaatgttaat agtctaatat93300cttactctat taaaatataa tcatgtacag agacttacac atatgtgaaa gtgtagacat93360atgtttattt acaaaagtag tattctgtat atacatttgc aacatctttt tctcatttta93420caatacaaga agaacacact gtaggtcagt aaatgtggat ctaccacatt ctatacagta93480actacctaat atcataatat ggctatacca tagtttatat aaccattatt tattgatggg93540
cattcaggtt attgtaagtt tttgtgggtt ttactgtaat aaatattctt aaacttagag93600ctacatatta gtgtttgtat agtagagctt tcccaaaata gaatgtgtgg ggcagagagt93660atatatctgt cttaaatttt aacagataat tcacattgtt ttaaaaatgt tataggaatt93720tgtttatact ccctagacta atgtataaga gtagtgtttt cctctagcat cacctgtatt93780cattattctt ttatttattt atttatttat ttattttttg tagaggcagg atcttgctat93840gttgcccagg ctggcctcga acaactgggc ccaagtgacc ctcctgcctc agcctcccga93900gtagctagga ctgtacaggc atgtgccacc acacctgggt ttagttattc tttttaattc93960ttggaaaaca gtggtgaaca atgttatctc attgttaatt taaataacac aaaatcatat94020tctgtgtaaa attatttcat aaatctcatt ttaatggctg cgtattattc catttagtag94080atgtattata gtttttaatt actttcattt gggtattttt aaaatgttgc tataatgaat94140aaagttgtag taaacacctt tgaggtgaaa tcctttaaaa atattttaga gttttcttta94200ggatagattc ctagggtgca gttactagat caaaggataa taatttttat gatcttgaaa94260tatatcacca agagctttct gaaaggcttg tactatttaa cactgccact tattccatgc94320tttttctgcc tacctgccta cagatgaact ctgaattacc cattgtctac acatgagtgt94380agcttctatc taaacctggt tatctcatac ccctatttta ctcactcctc tcccaatgaa94440agaaataaat caccaagttc tatttataca gccttcttaa ttctttaata gctttttaat94500atctactttt cagaatctta ctgtttcctt agctgaggct tcttttcttt gcctatctga94560cagcaaaaag ccttttaact ggccttacct ccagtcttat cttctaagcc attagccacc94620ttctttccag aatattcatt ctgcaataca gatcagatca gtagcacttc tctgtttaaa94680accaaaaggc tattcatagt ccttaggatg aaggatatac ttttatctct acagcctcat94740ctctctctat tctcatttct tttcccttgc ccttcctttt tttcccctac ctctgaacct94800ttatgctact tgttacctcc tttatttgac ctcatcttat gtggtggttt cttttatctc94860tacactgtct accttcccac cctcaagttt tgttagcttc tcctctagtt tgtgtgcctc94920cttttgttat agcatactgt cataatttct tgtttagttg tgtgtaaacc ctccctcccc94980tcattagaat gtgagcttct tgaagcagag attgtatctt gtttatcaag ggttcccaga95040acttcacatg gtacctgtta gtaccatgat ttgtagtata tttttgttga ctaattgaaa95100gcaggttagg gacttatcat ctctagaatg aagttttgtg gtataaaaag gttttaatta95160tctgtttttt tgcatacctc tccaagcaaa tttcccaaga cttttgctcc atcacatata95220
ctctgtaaaa gtcagctaca tgtattttta atgactatac aatattgttt aatatcttta95280tgcttttata aaatcctaat ttatatccta ttccttttaa ctggaatgcc ctctcttcct95340ttccttttct cttgttaaac tttttgtcat tctttgaaac cttgctgatt gtcctgagtt95400tatttagtat cttttcttac ttcaggacat aatgtgtata gatttctttt atagcattta95460ttgtaatatt tcatagtttg tgtttttatt tctgtttttt tccctagtgg actgaattcc95520agtgagacct gtatctttgt cattatattc atttcaccta gtgtctgcta aataaaagat95580aatcagaaaa tttggtgaat aaattagtga ctcaactata tttaaagaaa cattatttta95640gcccatggga tacaatagta ttttcgagcc caattgcttt aatttttaga catgattgcc95700cccgtgcctt cttgatttat aaatcaaaag attctaaaaa gattgaattt agcaagacat95760taaacgggtg tgggtgtgat gtattttcat gaggagaaac aatattgtag gcagaccata95820gaaatcagaa aatggtaata taacaatatt attaccttaa tatctataac ttttttatat95880agttggaaaa tactttttat agtaatttat atatctgctt tgtaacaatt gagaaatcat95940gcatgttaag aaaataaggc attttgttca tatttgttca tatttgaaat atcaatttta96000ttacatttta gaaatggaac tcagtttaca taaccttagc ctgagaaaat aattggtact96060gcattattga gaacttattc ttctgttaac attaaattag catgtttcta tctgtagtag96120tcatagaata tgttattcat ggtttttcat acaatttgag gtatttgcat ttttgtttat96180atctagaaac ataattgaaa tatggttcct ttacccactt gtacatttaa tgaagggtac96240tttgatattt aatttgacat ttatgaaatt ttattagagg gcagtaggct caatttgtcc96300catcattaga aaaattaaac tgaagatcaa tagagaatca tgaaaaaact tgttttaaat96360aaaaatgttc agtgcttata tagtgtcata attcctgttt cagttaatgg taacctgtca96420aatttagagt gttttcaaat ttgaagacat ttttgatgtt gttaaaactc aaggaaacta96480tagttctgct gtataagaaa aaaacctaaa attttaagtt gtaactaaaa tttatttttc96540ttttcaaggt aaataaatgt taatgggaaa aatgatttat tttattttat taaaataaat96600ttggctgtat attttttcct taaataccat ataagtggaa agagctataa tctgtgtggg96660tatgtattta tatatgtata tatatatgta catagtggca ctttagaaga aatgatttaa96720tgaaagatgt ttccaaaatg ttatttgggt taggtgcccc ctttaccatc aatataaagc96780ccaagctcct ttctctctct ctctctctct cgttctctct ctctctctgt gtgtatacat96840atatctatat gcagaatata gacatttttg ttttgttttt gatcatctgg ttttcttaac96900
ctctatttat gatttgtagt aaactcggta acatttttat tttggttttg tagcaagatt96960aatacaaata ttaaacctgg aagagcttgt taaaacttaa tggttaattt aaaaattagt97020ttttatgttt caatggaaaa gatatctgtc ttcaacgaat acatggttta ctgactttga97080atatatatga tattaaaaaa gtatttgtaa gataatttga ttgatttaaa ttgaacttct97140tgattatcta tttgagacta ttagtaacta cacaataatt ttatgagtat acaagacggg97200ttttgtactt tataaattat atttaagata ttaactttta tgtgaaatgg tgattaggtt97260atgggtgggg gtggggggct ggagtgggga aattggaaga aaagtataat gtccaaaacc97320gttaagaata aatttttatg ctttttagtt tgaattattt tttgttcatt atagtgtttt97380cattttgtga gtttttctat gcacatacaa aatgtttctc ctcaggcccc cttgtatttg97440ctggtcctat ttttatgaac catcgagaac aggctctagc cagactcaga tcccatccag97500cacagctaaa gcataaacgg gacaagcaca aaggtatttg gtcttcctta tcacctaggt97560gggatacttt cccaatttct tccactcatt tgaattttgt ctctgggaaa atgcacaaac97620ctgattcttc tactttgctt ctatcatgat gtagcctgag gagaaagaac attctgaaag97680tgataccgtt tgtcagtgac tgtcatgctt cttgtttttt tgttgtcgtt agctttcata97740aaggagatat tttacaatat atcttttcct tgtccatttc ttttcaaatt tcaaaaagtt97800aagggaaggt ggaaaaaata attattgctt tctgtcttac atcctttatt atttgctctt97860taatgactgt gatctggcct tttacaattc ttttttgaat gctgtttata tgctttagga97920taaacaatat tactaaattc agtatattaa atgatgataa taagactgcc attacacttt97980tccactgttt attaattaag tttatataaa caacaaatga atatacaaat atttgatggt98040tgtgggttaa gtagagataa gtgcaagcaa agcacgaatc atgaagactt tctctagtcc98100ctggtgatgg tcagagggct atcctagtct gtgaggaagt aggaatctag ctcggtgttg98160tcctttgaga tctcagagtt ctgggttaat taaatatatt cttaaagcat gcttattttc98220ttgtaaacca caaattatgg gtcagctaag atcaggaaag aatttaatac aaggaattta98280taaatttata taaaattttt gccttgtatc ctggcctttg aaccagtgtt cttccttagc98340tagcacaact taaaactccc attttcttaa atgcctaaga taggtcctgc aaaatagaat98400aggaagtttt aaaaacatgc tttccatgta gacaaattaa tctaattaat gtttggatcc98460ttttcttttt tttgctgcaa aattgtcata gtctaattta aaaaaatttc ataggcaata98520tagtataatc cactttattt ttatttgcct ctttcagtct gtcagtttgc cactcaaaac98580
aaaaacataa acaaaataga caaaactcat ctttagtaaa ggtgtaaata gagcacaact98640taagatatac tctgcagaaa taaaaatagt gttttaaatc taatgcaatt aaccacagta98700aaatttgtga ttactttatt tactgagtgc tctaacaatg ccataaatat ttaaggaatt98760ttcaacctaa gacagctgat tccaaaaaga aaagatacat atataaggaa taatatttgg98820ggtatgcaat gaaattgtta tttgccagaa tgtctgatct tttatgattt ctactatgtc98880acaattagaa cagatttttt cagtttcttt cttcccccaa atatatgaaa gtgaagtttt98940cccaatagtt taactgaggt taaatattca catttcatga aacagtttaa aaccttttca99000gctccatgtt agtatctcat ggacaagtat ctttaccctt tccagatttt taaatgaaac99060gttaaatgaa aaccaaggtt acatatttaa agcaaacttt tcccaatgtc acataatgat99120tggctataga aattattcag tgaggctggg cgcagtggca catgcctgta attgcagcac99180tttggaaggc tgagggggtg gatcacttga ggtcaggagt tcgaggccag cctggctaac99240atggcgaaac ctcatctcta ctaaaaatac aaataataat gataacaata ataagcccag99300tgtggtggca catgcctgta atcccaggta ctcaggaagc tgaggcatga gaacaataat99360cacttgagcc tgggaggcag aggttgcagt gaaaaaacaa gttattcaat gatagctagt99420cttcaagttt gcttgtcatg gggttacttt ataacaagtt tctttgtata cttgtaacca99480ctctgtaagg acctactttg taatatcaca gtgtaggacc agttttattg gattttaggg99540ttttatagag tgagatagtt ttatcttttt actccccaaa tacatttaca atgcaaataa99600taatttatca attggtaccc tatttttttg tgaagagtat taatttactg ctcactaatt99660tccctccagc cactaaccat ttacactcac tgtcttgtca tttaattatc tctccattca99720ctgcttttac ttttacgaca tgttgaacat ccctaatttg aaaattcgaa atgctccaag99780atctgaaact ttttgagcac caacatgaag cccccaagtg ggaaattcca cacctgacct99840cctgtgacag gttgcagtcc cagcacacag cttattcagt gtcctaaagt gaaagcaatc99900ctctcagccc tcttcagcta tgataaagct tttccatgca cagcatgatg gtgacgccaa99960tcacagtttg tctacgtggg tggctgggtg gctgaggtac ctttgctttc tgatagtgca100020gggatacaaa ttttcatgca ccaaattatt taaaatattg cataaaatta tctttcggct100080atgtgagtaa tttgtatatg aaatatcaat gaatttcatg tttagacttg ggtcccatcc100140ccaagatatc tcataatgta tatgcaaata ttccaaaatt tgaacaaact ccaaaatcca100200aaacacttct ggtcccaagc atttcaaata agggatgctc aacctgtaca tattttcagt100260
cttttaataa gtattttttc tacatatatt tttctcttca ccttacccag tttatcttct100320agtttatcta ctgagaagtt tatggtattg attgctatgt tctatcctac atttaattat100380gttggtacaa attataggat ggtaatagtt aaaatttttt agactgtgac ttttttgtga100440gggtgtaagg agagtaagaa ctgtggattt attgttaaca ggactaagtt ctaagatgga100500aagttggaga acttctgggt gattggagaa aattcagaga aggtcagaat tcatcctata100560caggcagata ttgactaaag gcaaagcaga gtatatagac tgaatcaata agtaaaattc100620catataaaat acagtaaatt attctaaaat gtgcctataa actatattcg aaacagaatt100680aacatttgtt tcagctattc agtaccttac acagaaaaat gtgttaatag tgtacatagt100740taggaacatc tgaattgttc tagagatagt cctaaaatct attttaaaat gactgatttg100800aaaatctgaa ggcacactga taaacatctg agtctatttt actcttactt tctgtttgga100860aatggtgcag tgtgatgaaa atattatagc ctttggaatt aggcttcaat tttatttctg100920aatttgtcat ttactagaac tacatggctg tgcaaaatgt cttaatctct ttggcttcag100980ttttcttatt ttaaaaacaa gaatagtaat tcttaactga ttgtgttgct atgaagatta101040aaatatatgt gtgtagtctt tgcacaccca catatgtagt ctttgcacat ataaatcctc101100catgaatact agctttaata ttttgaattg aattatttgt tttctctttc agaattatct101160taaaggtaca atatttgaga tttattaaat gcatagttta tttttacaaa ggatttaagg101220tttcaaccat ttggtggtgg taaaatgcat gagccttatt tatgattaaa ctctaaattt101280gtcctaccat ttatgatttt gggctggtta cttaacccct taacccttca agccttagtt101340ttttcatctg taagatggac aaagaatacc tatattatca gattgcttta ttcattaaag101400aaagtaacaa tagtaaatat tgattgagca cttttcatat tttatttaat tctcatagga101460acctgatgag ttacatatca ttgttgctat attatagata ggaaagctag gaccagaagg101520tccagtgtcc agttgtcata caactagcaa atgagaagag ccaagattcc agtccaagtt101580cttaagttcc agtactccca agcactcatc cactgtggta tttaattgtt tacagtgttc101640agttagcaca cagcaagtgc ttagtaaatt gcaactactt ttgtccttgc tgatttgttt101700taatgatttt gctccaagta ctcactgtgt tctgtggtag tttctgttat atgcatgact101760gtaggattac cctccaaaag tatagatttt agtaaaaatt aaaattagaa gttagagtcc101820cttagatact gaaccctctt ttttctctgt gtatacaaat atgtgtatat aattatatat101880aatgtgtaca tacatataat attttagtta catagttgcc aaggctttgt agctactgta101940
gtttcagtgt tacatggtat acacacacac acacacacac acacacacac acacacaaca102000tattcatctt agggaaaata cagatgtgct catacttaaa tggccaatta taagattatt102060cacatctgtc aattttttat tgacaatgtt taatttttta tatagatcta agctttggtc102120ttattgctgc tcattaacac tttactgaat aatgagcttg aacagatttt tttttttttt102180ttgagacagg gtctcactct atcacctagg ctggagtgca atggcatgat cacggctcac102240tgcagccttg acctcccggg ctcaggtgat tctcccacct cagccaccca agtagctggg102300actgcaggta ttcaccacca cacccagcta gttttttgta tttttagtag agacagggtt102360ttaccatgtt gcccaggctg gtcttgagct cctaggctca agtaatctgc ctttctcagc102420ctcccaaagc cctgagatta caggtgtgaa ccaccatgcc cagcttgagc agaattaatc102480ttgctgaatt cacttgctgg ttcttgaatt tggactaaat ttttggccac ttattaagtg102540ccttttttct ttgataaact ttggttaatg tctgagttat aggaattata ctcaattttt102600tgatatcaaa taccatatac agatatatat ataaaatata tattttaatt ctgccactgc102660tgctgataaa gattaagacc ctcatctcaa tttttttact tgtaagattt tcactgtcta102720taataattga gaagttgttt tgtacagtta cagtttgttt caatgaacaa aatgtttcta102780gtattatttt gacctctaaa taacagctgc tactttttta gtcattgtgg ttgtattcac102840taaattacca acctttaaaa ataatctcct tccttgatgc tatgtctcta ttgtgggatt102900tatgcctata tttaatctcc tactcaaggg aggagacaaa atgctaaatt caaaggtaaa102960accttataac taggcatcaa tacatttata taaagtggaa tgatacttga aatgtttaat103020taagtctggc agaatcatat gaacacaatg tattttctat gttaaatctc atttggtttt103080aataattaga catttttacc aattgagata aagccgtaaa atttctttac atctggcgtc103140ttattaaata gcctttggaa gatatgtatt tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg103200tgtgtgtatg tgtgtgtgtg tgtgtaggct tttgcatata ttttaccatt ccactatctt103260tccaacatga ggaggctttg aactgagtat attttatttt ggatggagtg aaactaaatg103320ttgttggttt tttttttttt tttttagtcc atccattctt tgatttaatt tggcaaaccc103380acattagata atttagcaga agaggaatta tatcttcatc ctattatagt aaaacctctc103440actaattctg aatttatgat atttgagatg cagtatttgt gatctttttt gagtaaaaaa103500ttcaaaataa tttcttcctc tgaattttca gggtcatact tataagggga aacctgtcta103560aaatcactag cttttcaaac caagggctca agaaatgaag gattccttca acgtgccttc103620
agcctctggg tccagcagtt aattcttgta tgttaaagga cttttatgtt tatggtacat103680tttaaatgta atattggcct attttgaaat ataatgtagg taaccacact ctatttattg103740tagcatttag tttaatctgt aatctgtact gcttgaaggt aacaaaactg aatttttact103800tcagtgttct gtatgattaa gacgtttcca gtaagccaca caggcctacc tgttctatgt103860tattccgaat tagtgaggtt ttactgtcct agtgtcgtaa agagtttcag tcttcttgaa103920attataggca tttagttacg gaaaggaaag taataatgta ggtagatttt gtctcacctg103980gatgttacta aaggttaggt aaaaaaagaa taaacacaaa atgatttaat attattctgt104040ttaacattct aaaatgcaca atgaaaatac aggatagaaa gtgtgtgtct gtagattcac104100atatgtatct tcatataata cacacttgtt tagagttgct ataccacttg agagtggttt104160agactagttg aacttgtgaa attccttcca gatttacatt tattctgtgt acaaggaatt104220tgaatcttga tgatataaag gtcatatgtt agtgatgtat agaagtacat tgtgattaga104280aaaagtaagt tgttgttatt tacacattcc aaacaaaaat ttttatattc tggaatgtgg104340tttttgatgc tatggttaga ttgttggaaa attcaaagtt ggattgcaaa agccaagggt104400aaagaaaagg cttaaagcaa gctcaatgaa ggaaattgtg agaagggagg aggaaagaag104460gagtgaaagc agaaaaaagg tttggttgag ggtagtgatt ggggacaggg agaaaaccac104520aaagcaatat ggctgaaggt gatggtggca caggaaacat gaataagaat aggccattga104580actgcaatat attgaataaa gctctttggt ctttatacat tttgaaaagt tcagttgagt104640ttcaaaatac ttatgggcag tgtagccttt ctaagatcta tttaggaaat ctaagtcact104700tgtgaatcca ttagcaattt tttaaaaatg gctagtacta acaatatcac taagtttaaa104760ataattgtta aatgactttg catgcagtaa ttttttatgg aaactaaatt ttcccatata104820aactgttaca ttttaaattt tgttatttct ataacttaat gacccgttta ttacataagg104880ttttatccat aaccagatct tatctctgtt tttattaatt tagtgatatt tgcaaatgga104940agtatatagt ctatcttttc aaaattcctt ctttgcaggt agattcttga tctatcactt105000ctaacagcta cagattcttc ttgcttgttc cttttaacat ttttctcttg ttcttccata105060ataaactgat ttaaattttt attttgaaga tgaataatgg cttaatcata attcacaaat105120tataacactt gcagatggaa gtggagaaag aggcgaaaag gatgcaagca aaatcacaac105180ataccctcca ggctctgtgc gatttgactg tgagctccgg gcagtccaag tcagctgtgg105240atttcaccat tcaggtagcg gctggaactt tagggtataa ctgcgttttt acaattgtgc105300
tcagtaactt atttcttcag atttgatgaa catagcctgt taactgtgtg ttaaaagctg105360tagcgtggca gtctctcaaa tctgtaagac ctttgtattt attacagaat ctctttaaac105420aactatggca tgatggaaca ggtacttgtc tagagatcta acttctagtc tgtcttcctg105480tggacttctt ttataattca agacagtctg gaagatgaaa taatccttta aatctctttc105540agttctagaa tgctgtggtt tttgtaacat gttcccatat tttgttaatg ttcagtgata105600aatgtatgtc aacaaattta ctttcttaaa atgtagaaaa taattaaatt cttagtaaat105660tattggcatt tgggttagct tttgctgtac tgttcaacct ttataaaaac aaaaatgtta105720aattccttcc ttgaagatta tttgataagg tattaacaga cattaaagtt ctgctgtttc105780attgatgttg tacgtaattg tgcagacctc ctatcctaga taggagagaa caatatttta105840tatgtagtta ctcattatta agaagatttt acagttgacc tgaaccagtg tgttaataag105900aattaatgtt attaaatggc atttatacga atttttaagt gtgtattatg aaaaaattat105960acatttatat gacctacaaa aatatttttc agaactaaaa ttaatttctc attaaataca106020aatttgtcct tcagttacct atttttaatg gttctataaa tcttaaatag tgaaaattac106080actgctttta gtatagttgt gatagaaatg taaaatgcat ataatgaaaa aataaagctg106140tgtgttttgg ttgcagtggt tttaatggaa aatggagatg tctatacatt tggttatggg106200cagcatgggc agctaggaca tggagatgtc aactccaggt acttgctaac ttttcatttg106260aagatccaga gttatttttt cttctgtttt cttttccttc ttttttcttt cttttctctt106320tttaagtata tatttctaga attaaaataa tattatacca ctgctttgca aagtgtggcc106380catgaattat tggtggtttc tgatcttatg gttgagtgct tttgtagaga taaaggattt106440tgtagtttca ggagaaaagc tgatatatat tttaaatagg agagtgatta tgattgtcac106500aaaatatcac tatattctta gcaattatct ttttaaaggc tgtgcatggc aatagaaaaa106560tagtgaattg tatttttaaa aaatgtcagc aatggattca aacatattcc tgatgtctgt106620tttgaaattt tggtctaata acatgcagtc attgaagctt ttgaataatt tttggacaga106680acctattggt ctctctgatt aactcttttg acttattgca aatagtagta gagagactgg106740tggtaatttc ctaatatccc tacttcattt gttttagttt tagttttagt tcgtgacatg106800atcgtgcagc taaagattcc ctttctcagc ttccattgca gcaggatgtg gccatgtgaa106860cttaagtgat aggtgccact ttggaatctt gctgttaaag ggaaagggtg tacctttcca106920tttgtccttc tctcttccca gcttgctggc tggaatgaat atgtggaagc agaaactaaa106980
gaagtcatca cagagataag ttggagactg tgtactgaga atggcagaat cataagatgg107040gaataggctg ggtctctgac accatgaact gcctcatcag cttcttaaat tgcatgcttc107100ttgcgtgaga aataaacttg tatcttgaag tccctcttac ttgtctttca ttacagcagc107160cgagtttgta ttctacgtaa tgcagtcaga atacttttgg tcaaaagtaa tagaaaatcc107220atctttatta gttcaggtct tccagaaagc agatgccaag acaggattag atatgcaaag107280aatttattag atacatgtag ttttgaccct atgaaaggag aaagagaagg aagaatcggg107340taggagtcta ggttccagca taacattaag agagttttag ccaggccagt gatgtgtccc107400caagctgagg atacctttgg aagggtcctg cattgggcag gaatagacca acattcatac107460cttcaccatg ttcagtcaca gctggtagca ccccagggga cgaatgtcct cgtgcatcca107520aagggacagc acctgaggct gttataatag ttaactctgc tctctgtagc agattgtctt107580gaaggagatc tgagtggtct gtgtctgtgg ttgtctcacc atggaaacag gagtatttat107640gaagtctgga aagatagaga gcagcttcaa gccagacttc actcagcaat tcacttagct107700gcaaaaactt accttttgac cttctactct gccttattct tcctcgcagt cacagtgtat107760atgccagaat caaggggact tctatggttt ctcctttaca tacagtgaaa gaaagagaac107820ttaccttcac atagtcatta aataaaattc aaggttgtca ctgtgtcttg aactcactct107880actattgtgg cagggatgga attctgccat ttacatggta aagattagtg attcttaacc107940ctggctgtat attagaatta cttagggaat ttgaaaaaat atcatatgtg gattctacat108000ccagcatttc tgagagatgt gtgtgttgaa aggcttgtgg gtgggacagg ggtggggagt108060ggtccttgat aggatgccag catcagagtt ttttctaaaa ggtccccaga ttctatatgt108120atccaggatg agaaccactc tctacccttc gagctgaggc agggtcagat ctgtgggcac108180tgcataaaaa atggcaacaa aagggaatgt cctgagttat gtttatgaat gttgttgcta108240atgataagag acgattgaga tatcattgag ttgaacttct tattaaaaac acaactgaaa108300cataaaattg atactactat caagaggttt gtaaaactat cacaatcata aatatttaat108360gactaatatc atacttgcaa agagcaaaag atgtgtttgg cagaattact ttatcagatg108420tgtctgctgg atatgacata ataccaacag tgttgagcaa ttactgtcac aggtgttaga108480tgccaactga tattttgctt tgcaattgag taacattact gaaatgcaga ggctgaatct108540gggtagtaat tacagttggc cctctgtacc tgtgggttct gcatctgtgg attcaatcaa108600tcttgacaga aaacatagtt aggcctacga taattgcatc tgtactgcac atgtacagat108660
gtttttgtct tgttattatt tcctaaacaa cagagtataa caactattaa catggtgttt108720acattgtatt aggtattata agtaatctag agatgtttta aagtatacag gaggatgtgt108780agtttatgta caaatactat gccaatttat ataagggatt tgagcatccc tagagtttgg108840tagctgcagg ggatcctgga accaattccc catggatgct gagggatgac tataacctgt108900gtggggaaga acgtggcaac tttttgttct gtttctcatt tgagagttgg gacacagaag108960aagagggttt atttgtatca tcgtggctat tgttgttgtt atttagctgg tggttatttt109020gtgagccata gaaactggga aaggtgcatt gcagttagat aagatggggt ctcagtcatg109080tatgcagcaa agagggcact tcacagaaaa taattggcaa atagtatttc atggtcttgc109140cagagtggaa agggaagtag tgaaaagcat ggccatgtta tagtcaggtt atgttatctt109200actgtgcact tggcactcag aagaatgagc agcatgagca caacatcctt ccacaccaca109260ctgaggtgtg ttggccgctg agagggaagg tgttcatgtc agcttttaaa atgaagagat109320tacctaaaaa cattacttcg tggtcctgat tataccagtg aacaccattc ttcgattccc109380aagtctgaag agatataggg cagtttcagg ccagacttcc tcaggtaata atattgtagc109440aatatgctca atattttaaa ttgcttgaat ttatcgtctt catgaaatac gatagcattt109500aatgttgaaa caaaatatca gggtttctta tgaagattaa acattggtag gatctacaca109560gtggtatagt aactggtaga tgctacatag tcatcgaacc tggaactgta gaggcaaggt109620tcagctcttc acttcttacc aaccatatga ctataatttc ttaagattaa acactggtgg109680gatttgtttt aactgcccaa agttgttctt ttccagcatg gaatgttttg gtccttgttc109740cattgagtgg taactgaaag actgattaca tgaagtgtgg gcagagctac aacggataat109800gcagtagtca tacctatata ctgtgcctag tggattagat attcatcaag accgcttcag109860tcttaagcac tcagttaccc tgtctgtctg gacttttgtg gaacttggaa agtagcaacc109920aagaactctg ttattaattc agaaaggagt tgattatgtc tatagaggaa caatcttggg109980aggaacaaag cttctatagc atatgtatta actacaacaa agcaaaacat ttacagatag110040ttgaaataaa gggttacagt tggagaatat gtttgaaaag tctctagtaa tcaagagctg110100ttaactggaa tttactatat agcacacatt aataatgtgt tgtttaagat caatattgtg110160gtatgcatga taatgaagat ggaaaagtgt tattgatatt ctctttattt aatgtgttat110220attgccagaa tgactgagtt cagtctctag gttgggtcct gattctgtca cttggtagct110280atatgatctt ggccaggtta cttaatcttc ttgtgtctca gtttctcaag tttaaatatc110340
cataatctga tgatactttc ttcttctgtg atgcagccct actttatttc cctttgactt110400tatgccccac agccctctac ttttttactc ttttttcacc ctttttttaa ctttatttcc110460tactcaagtt aaagactgtt gcttaatttc ttcacctata ctcttaccag tttcttcagt110520tttcccactc tcttggtcgt ggtgctagga catgataaaa ccgcaaacat gaatcaatct110580taatatttcc tatgcctata cttaggcttc tggaaggtaa gaaatcacca tacctatgca110640gattgatact taatttccag attcaaccag ttcctgtaca atggtcatta atcctcctgt110700tgtgtagtat tgaactattt gacttaatac cttttgagag tttgatgaaa gacatggggt110760ctctatgaaa aatacaagaa tatacactta aaaatttgct tgcaaattta ggagatcttt110820tgaagctcac tcatggactt cctagaagtc ctttatatct gagttgaaaa ctcctgctct110880cggtatgtat ctattccatc taccaccaca ccttttctcg aatcttcaaa ctctccactc110940acctgtttcc tccttttagc ataggatctc catgtacatt gcaacagatgtaagaaatta111000ttcagcccac caaccctgca aacctacttc catccatacc acttctctgc ttctttcctc111060atgttacagt taaagaggaa cctgttgtgt ccttgctatg aaacctagct gtcttttcct111120tagagatcta tttctgtttc ctgtcttctc tctaaccttt gtctttagcg tctttatttt111180gagtcacctt ttaaacttcc tcaaatctct tatcattaaa aaaaaaaaat cctctccttc111240agcttcattt ttcctttagc tgctggctct cctttctttt tccctttagt gctgggatac111300ttagagctac tttgtgtatt ctctgactcc ttatctgctg cattgtggtg cctgcttcca111360ccaaagagac ctccttctgc taaatcgagt ggttaggtgt atgtctcctt agagcatttg111420gcatgtactt tattttccac gcttgaaacc tactcctcca atgagtctcc ttgagttctt111480tctttctgtg tggtttattc tagatgtctg tgagctgttc ttcctcggtc tgtatctaaa111540atgttagaag agactcataa gggaaattag gatctcttct tttctctctc aaagagtctt111600cctagatctc attcattctc atagcttaag tgaatattac cactatctac tctgttgctt111660aagcaaaaaa aatcttcctt gccccatatc gatcatctgc caagtttcat caattctgtt111720ccttacatag ctatcaaagt caccatctct ttcactctgc actgctccta gttaaggcct111780tcatcataaa ttacccaaac tactatataa tttttaaaat ttaaagtgtc ataattacat111840ttctcaaaag cagagctctc ctgctatgtg tggactattt ctataatggt tgtgtttcta111900attacagata atggttacta aaaatggctg ttaaaagtag gaaaggatac taggatgtca111960tttatcataa tagttactgt tcattaagtg ttaactgtgt gccagacgct gatccaagta112020
cttgacacgt atgggcctct ttactgttca caggaactct gtgggggtag acaccgtttg112080ttagtattct ccatgtgata aagaggaaac tgaaatacaa aaagaatgaa aaacttgttc112140gaagtctggt cgcattatta ttaagttggg gagccaggat tcacaaccag ccagtctagc112200tttaaagcct gagcttgtag tcactgctct aatgcctctc atgatactgt gttttgactc112260attaactgtg attctaagat ctaaacttat agcttagtga taaaagttag aaaagatgat112320cataaaatta tttcatctag ttcctggatt gaatttctgt taaggcatta catgattttt112380atttcatcca ataattattt ataaagatct ctaacaggcc agtcagtata cagagtacca112440gattaaaaat aaatgtagca ccagtttttc agaaattatt atgtgtctat aattagggta112500attacattta gaagatcttt ttgatgatct ccttaaagtc agcaactgtc tttttcatct112560ttgtttacct agtacctgga atggagatag gcgtttagca cttaaatgtt tactgaatat112620tcttatgagt gccttttatc tttcctactc cttgttgcat tgcttactta ttgtttttat112680tttagttgag ttttgtaaga aattgactta cttttttttt ttttaaccta ggggatgtcc112740cactcttgtt caagcattgc caggccctag cacacaagtc actgcaggca gcaaccatac112800ggcagtactt ttaatggatg gacaggtctt cacatttgga agtttttctg taaggaattt112860ttaaaacatt aataatattg cattatacca ttgccttata atttgtctat attagctctt112920ttttctgttt ccagaatata atataacaat tatattataa ttgttacata tgcatatttc112980atgccttcat ccccaacaca cactaaacct gaatgatatt ctttgaagta attttcctct113040ctagctcagc attagaattt attgaattta acagctttgt tagaattgga catgtttatt113100tcagattaaa gtcttttaag cattcaatag agctaattct gtcataggaa aggttatttc113160tcatctaact tgtagagatg aatttttctt aacacataga actatgctat tttgtaacct113220tttaaaagcc tagttttttt tatttgattt gtttaaaatt atactttctt ttttcctttt113280ccaccctctg agtctatccg cctgtctgtt acataggcgc ttgtgcacat tctctccctc113340tctctgcacc aaagcttaaa gagtgaaaat gctctaagaa ttttgtgtag tttgggcata113400gtagatatca agaaaaatct ttgcgagact gtgctaatac tcttgtacca tttcagatgt113460ggataattac taagaactct tgaacctaag tgtctgagat gacatttaca gcttttgatt113520ttttaaaaac tgtaaatgtg tacttaaaat attttatttg aaaatggttt caaacttaca113580gaaagattgc acaactaaga acagtgtgta gaatatctgg ttagtgtctt taaccagatt113640cacctattgt taacattttg atccagcatt tgctttatta ttgcacatgt ggctgttctc113700
tttctcacac acgcagtgtg tgtgtatgca caaatgtgtg tgtgtacgta tgtatataaa113760atattttttt ttctcaaatc ctttgacaat gtcttgtgta cattatggcc cgttacctcc113820tacatacttt agtttgtatt ttgtgaattg acctaataat tattttttgg catttctttc113880ccctccaatc taggatcagg cattacattt gcctgttgtg tgtctttagt ctcctttaat113940ctgtaacatt tctacagcta tgtattgtct attataacat tgacattttt gaagagtata114000tagtactgtt ttgaaaaata gaatcctctt catttttgtt tctttgataa tgtcctcatg114060tttagatgca ggttgtgcac ccaggccaag atactctgta agttatgttg tgtttgccct114120cactgcatca catctgaagg ctcagaatgt ccatctgctc ttcattgatt atggacattt114180tgatcacctg atcaaatgtc tgatttctgt actgtttctc ctttgaaact ataagcaacc114240tgtaggttga cacttttaag atgcctgctt ctcatcaaaa tttcccccta gatttagcat114300ccattgatca ttctttcctg agctagtctt tactataatg tttaaaaaat ggtgattttt114360ttcaactcca gtaggcactt ggccttttac tctaagcaag agccctcctt tcctgtttac114420ttgtttttct acttattatt ggtatagact caggtatttg tattttcttc attatcatct114480ttaattattt gggtgcctag attatctccg atttggccag cagtagcccc ttcaagttgg114540ctcctgtatt cttgtgacat gccccttcat tttttggagt gcttcctttc tttccagcat114600aacaagttgt tcagggttta tcttgtacag gccctgtatg actgtcctag aatcagccat114660ttctccaaga accctgattc tttttagtgg ggaatagtat tagacactaa ctgggcacta114720gaagtgttca ttgctacagg acgtctttgg atctctggta tgcatatatt tatatgtcca114780catatatgta tgtattttag aaatcatagg ccaggtgtgg tggctcatac ctataatccc114840agcactttgg caggccagtg taggaggatc actcaagccc aggagtttga gaccagcctg114900gacaacatag tgagacccca tctctattaa aaaaattagc tgggtgccgt ggcacatgcc114960agtggtccta gctgctcagg aggttgaggt aggaggatca cttgagccct ggaggtcgag115020gctgtagtga gagctgtgat tccatcattg ccctctggcc tggacagcag agcaagatcc115080tgtctcaaaa agaaagaaaa agaaccccac tgtgacttct acttccaatc cattcccaca115140aggttctttc ttacctgtcc tgttctatat ttatgtactt tcttcctcag taagaatctt115200tcctctcaac aatatcaata tatttatttc tcacttagtc cccagatatg tctaaaatag115260ttttatattt gctttgcctg tacaagttca aaaaaacaaa ctcactgaaa ggtcattctt115320gctctttccc ctcccacctg ctccccaccc caagtctgaa ggtgtatagt caagcactgt115380
ccatgagtgg cctggattct ctctctcact cctttcagtg gaaattgtga ttcctttgga115440atagagtggg gctcatttgt ttcagtttaa gattcccctc accctatcct ttgattaagt115500tttatttcat ttttaaaata tatagaacat ttacatgttg ctaaaaatta agttatacaa115560aaattgtata ctcaagaggt gtcacttcct cccatatcct tgacattcct gcctccccac115620acctttcatt ccatacttgg aggtaatgaa tgtcattgtt ttacagttta ttcttcttgt115680gtttcttctt gtgaagatat ataagcatgc agacacacta ttttatatat atatacctat115740atatgtatgt atgtgtatac acagttatgc actgtataac agtattttgg tcagtgatgg115800actgcgtatg ctaaggtgtt cccataagat tataataagt actttgacta taacttttct115860atgtttacat gcagaaatac ttactaatgg gttacagttg cctacagtat tcagtacacc115920agcatgctgt acaggttggt agcctaggag caataggcta caccatatca cctaagtatg115980tagtagatat accgtctagg tttgtataag tccactctga tgttcgcagc atcacagaat116040cacctaatga cacatatttc agaacatatc cctgtcatta agtgacatgt gactgtacat116100aaatatatat atttcccctt tcccgttttt taaaggtaat agcctatata tgctcttttg116160cactttgctc ttttcgcttc agcatctctc ctagaaatca ctccatatca attcataaag116220ctcttcattt ttttttttac ctccatgtaa taccccattg tgtgtatata tgatagttta116280ttcattcagt ttctcatgtt tatatattta cgtggtttcc aatactttgt aatggtagta116340gtgaatattg ttagaggtgt atctttgaga taaattccta gaagtgagat tactgtgttg116400aaggttaatg cctatgtagt tttgtgagat attaacaatt ctcctgtatt ttgctttcac116460actaacggtg tatgagattg cctgtttcca cagaattgcc aacagaatgt gttgtggtac116520ctttaatttg tgccagtctg atgggtgagg aatggtcctg cttacttcca agttcctttg116580cctttagttg gtgctctgat cctcaaagtt cggatccata tttagtattt tggcattgaa116640ggttaacttt ctttttgggg gcatgtgttt ccctgtgctt tttctaactt cttcacacgc116700ctctgtcctc cttcttaaga acttccctgt tcgtgctttg cacatgctca ggtttgcagt116760agccggtggg tggagagaac tcttggaatt tggctcttct gcttacagga aacatagaga116820tcatgacacc cgctgtctcc ttccactgct gaaggtttga gtcacatata gatttgtttg116880cacagcatat ttttgtgtct tttgaggtgg ttatgtgagt gataagattt gacgtcagac116940agtcattgtc ctccagtccc agcagtccta gttgtgaact tttatgaatt taaatgtggc117000ttatttataa ccacattctg gtccttatgg ggttgtccag gtatctaaca catttttgga117060
ctgcttttct tcatattagt tggtagtaag ggaatacata tttatttcaa tagtcaatga117120aaaagataac taatttattt ttcctccaaa agcagaatat atccctgaat atgatatatt117180ttgctcacta tttacttttt tttttctttt tgagacagag cctcactctg tcatccaggc117240tagaatgtag tggcatgatg atctcagctc actgaaacct ccggctccca gattcaagca117300attcttatgc ctcagcctcc tgagtagctg ggattacagg cccatgccac cacacccagc117360taattttcgt atttttagta gagacgaggt ttcaccatgt tggccaggct ggtctcaaac117420acctgacctc aggtgatcct cccatctcgg cctcccaaag tgctgggatt acaggcgtga117480gctactgcac ctggcccctg tttacatttt tgtgctctct tatttttgtc aaaaaataag117540gacagatctt aaaagagtat gagaaataaa tgttgagtgt tttggctgct tctctaatca117600tgtgcttgcc atgtttcctt ccctggttct gtccagggat tgaagctcag ttgcttccac117660agttagtttg tggctgtgtt ttcttgctgt gctacctggt ggttggatga gaacgtgacc117720agagtgttcg cagtgctcca ggctcccttg ttccagaatg ctgtgagggg agagaggtcc117780cgagccaggg gtggaagaat atctattgag tcaatgtcag cattctttta ttctttttca117840gctacaggct tactagtgag aaacttccta ttacttttta aattaaactc ttacctgacc117900aggtccctat agagagccat acttttaaac tggttaatag ttatttactt aaaatatcat117960atcttaccat tgattgtgct gtttatttta aaacataaat gaaataattt agggacatat118020catgtgaaaa caacattaca tatttttcta attgtattca cagtttcagt atttattttg118080gtctttttac atggatattc tcagattata tgagttcaat agtgactttc ttttatacta118140tcaacttact atgtcaaagt gactttttaa atgaacaatt tggaatacta atattttaac118200atattgtgtg tatttgtcgt acttttcatt taaatgtagg tgaagacaaa gtattttgga118260gatacttctt gcgtggaaaa tatttcaaac ttttttttaa aaaataatgc actttttaag118320actttccagg aataatagaa attattttat tactttataa atatttggtc caaattctta118380catattgtat ttattttaaa aacccaaact aacaattggt gaaaaataag gtttcataga118440ttgattttta tctactgcct ccttaacttt atgaaaatta gattgtttac acagcaatat118500tgctaatatg gtttatttaa tcttttagaa aggacaactg ggcagaccaa ttttggatgt118560gccatattgg aatgcaaagc cagctcccat gcctaacatt ggatcaaaat atggaagaaa118620agctacttgg ataggtgcaa gtggggacca aactttttta cgaattgatg aagcacttat118680taattctcat gtacttgcta catcagaaat ttttgccagt aaacacataa taggtaatac118740
cagaaataaa caaatgcccc ttccaaactc ttgtcttgat tgatagtaaa tggtttatct118800ttccctttaa tgaaatgtac ttttgtagct ttttgttttc tttctgctca aagaaattta118860aacaaagcct gtaaatgtct ccgtttatat gtgtctctat gctgaaagaa tggaataatt118920gggagatctg gaaaccacag tatttgtgag ctagacagtg gtagaactat aaactaaatt118980ctagtaggat aaaatccata ttagagtaag agagttttat gcattgttta ttgctaaata119040aatgttagaa attaattttt atgggaggtt atataaatcc aactttgaaa tttcacgaag119100accaagttat caatctgtac tgtgtccatg atactgtgaa taattatata atagagtggt119160atagcaagag attaggagca tgtgttgtgg atttagacat cttgaggttt tattcctaat119220tctcccactt aaagattttt taattactta gcctctttta ttctctgctt attcacctga119280gaaatggaga taatactact ttatgggttt tggagaattt aaaagggtaa tatatattaa119340aattatatat aaatattttt ttcttaaggc ttggtacctg cttctatatc agaacctcct119400ccatttaaat gccttctgat aaataaagtg gatgggagtt gtaaaacttt taatgactca119460gaacaagagg atctgcaagg atttggtgtg tgtcttgatc ctgtatatga tgtaatttgg119520aggtaagcat ctcagtttat aaaatagtca ataagttttg atgcagttat actcttatgg119580taatataaac tttatttaca ggtggacttt tcacatgtga gtactctcct gtttatctaa119640taactgcaat tctgtctcag tgattcacag ggacctttat actgtagcat agtggttaag119700agcatgcatt ttaagcaaca ctacctaggt ttatcccagc tccacaactt gctttctgtg119760tcaccctgga gaagttactt tgactgtgtg cctcagtttt ctcatttatg aaatggggat119820gctaatacta cagaggtgca tactaggtgc catataagtg tttgctatta tcattataat119880tattaatttt actgttgaga gttctgttgt tatttttagt ctgtcatcaa tcctgtttat119940attgtataga attcattcta ttttttgttc tcataattat tattaactat gaggaggaaa120000ttatgccaaa gacaataaag aataaagaag ataattgagc ttgcccttta agaatgtgca120060ttaaaaaata ctcttactgg tagagaaata tacaggatta tatgcattct ggaaattata120120aaaacataga gataaataag gcacaaaaga gactcaagaa attatgttgc atttgtttga120180aatttattgt ggtgctgggc cgggtgcggt ggctcacgcc tgtaatccca gcactttggg120240aggccaaggc gggcagatca cgaggtcagg agatcgagac catcctggct aacacagtga120300aaccctgtct ctactaaaaa tacgaaaaat tagccgggcg tggtggcggg cacctgtaat120360cccagctact cgggaggctg aggcaggaga atggcgtgaa cctgggaggc ggaatttgca120420
gtgagccgag atcgcaccac tgcactccag cctgggcgag actctgtctc aaaaaaaaaa120480aaaaagaaaa aaaaattatt gtggtgcttt atatacaaaa tgaataaata agttaccctt120540cttagtgttt ctagctttgg atactaatag gaggtgtacc ctgccatcac cacagtggct120600catcacaggg ctgagcactt ctttcctttt gcggccggcc ttagtaggca agattccagt120660aagttgaaag gttgaagtag tgtgagtgag gagacttttt ctctgtcttc cttcttctac120720ccatgcctgc ttgcattctc atcagacagc caagttctaa gaggccatgt ggtagcaatg120780ggatagatga tctcaaatgt cttgccaact ccttggtaat tttcttccgt gttccaaaat120840agagattaat aacgaggctt gcattcagag tgaagagact gctgcacttc ccaggttgct120900ggccagcttc ctctcttatt tgggcatttt atgagaaggc gtagtggcca gtattgagtt120960gagtacatct caaatcaatt acatctccct ggcagcattg tgcagggtag atgaaataac121020aagagactat gggtgagtgg tcttttgtga tcattcttat gaagacatga ggtattaaga121080accaaagtta gaatggtcac agtgggatag aatggagggg atagagagga gaaaagatgt121140aaagatagaa ttggctgcag ttacttaccg attagaagtc aatgttcaag agacttagtt121200accaaagagc aggctctggt ttggacttag gtgtaaattg atgttttact gttttgtagt121260tcttacatgt tttattttta aatgaagtaa atttgcatta gcaaaacaaa aataaaaatt121320ggcagtgtgg aatctgtttc tggatgcatt cactaagtta gcaataacag tataactaag121380cgtaacagta aaaacttact tgaactagaa ccatttccgt ggggtcttaa cagaactctc121440aagtatgatg ttcagctggt tgaaccttgt cttttccatt tatggtagta gcaatgggac121500agatgatctc aaatgccctg ccagctcctt gatcaatttc tccattgttg gaaaacagag121560attaagagat gttttggtaa aacagctgag aggtattttt ctttaaatca ggatcagaat121620ttctgttatc ctgacttctg gctgctttgt gtcttttgtg tttttcaatg gggcacttaa121680attttataat tatcatatgc attgcacatc caatattagc ttactttgac tttccttctg121740gcctgcatct gcttcatttt atattcttta ggagccaact ttgcctgagt aattagaaac121800aacatctata aagagcagat aaatgttaag aacacctgtt ttatttccca catacaaatt121860tacacaaata atatgtgaat ttgtgttatt ttttaaaaaa caaagcaaaa atacatacag121920gaaaaaggat ggtcatactc tgtatctgtt tcttttccca taccgaattc cactagcctt121980ctctgtggat cacaagtatg aagtttgatt tatatcatct gacacctttt aaaatgcatg122040tatttttctt tttatgtaaa taaaaacata cctactctaa aagttagatt ttttgctcaa122100
aaataattta gagatctttc caagtcagta tttaaagaac agcttcatta ttaaaattgt122160ggaattctgt agtctgggta taccaaatat acttaactgt ttgcccatca gtgaacattt122220tagatttttt ttaatattac gatactacct acaatgctac agtgaacaaa tacacaggta122280tctacacaca gagttttaca cacatgtgga agtatttctg caagctagct tttaaaatgt122340agaattgctg agttaaaaga taggctcaca gaattgtaaa atgactgagg taagtctgaa122400tcactttaga agtttatttt tccaaggttg aggagatgcc tgggaagaaa agacaagcca122460cagcaggatc tgtgccctgt actttttctg aagaggtttg aggccttcag catttaaaga122520ggaaaagaga gcaggaggag aaaaggaaaa gaaaaaatga gaggatgtgg tcacattctt122580gtaaggtttt gattaggctt actgaatcca catgttgcac atgaaaagga aggggtagag122640ggaacagtga attttgtatt tggagttaaa gtaaacatag agtagaggaa gcagtcaaat122700actcattcat ctggggctgg ggcgggtggg gcttggtggg gggatgggca gataatttct122760agtatcttct tgtctcttac catgaatttt ccagaaccaa aaagagattt gaatccatgg122820atattagaaa ctatgtgttt ctattattta ccatcaagag agttagtgaa actgttgaac122880actaaagacc aaggggaaat cttaaaagca accagagaga aaagaatgat tacctaaaca122940atgagatcaa cagaacttcc caaaagcaat aatgcaataa tattaatgct actagttata123000gaagctacca taatatattt tctcccaaat gctattttaa gtgcttttta aaaataattt123060aaaatttttt aattgtggta aaaaaacagt ataaaattta ccatcttaat cattttgaaa123120gtctgcagtc agtggtgttt agtgtattta tgttattgtg aaatagatca tcagaacttt123180tttttcttgg aaaactgaga ctctatagtt attaaacaat agcttgcatc cctcacttcc123240cgacccctaa aagaggtaaa gaagtggaga tagttttttg aagtttttgt tacaaaagag123300atcagagaaa tgggatgatg aagacgttgt gggttcctaa tagtttttaa ttggttttta123360ctgtttattc atatggttga acatccacat ccattctatc tgtgcttcct ttctatggat123420agcattccat gtccttgcca atttttgcta tggattgatt gtttctagct cttatacatt123480gaggatattg tagctgtgtc tcacaggtac acttcacatt tttcttcaac ttgtcctttg123540tctttaaact tttttaatgg ggtatttgtt gagtttcata tttatatgta tgtatcaaaa123600ctttatacag tcaagtatta taatctacat tactattttc tgtattaagt attataatct123660acgttactat tttcaaatta tttcactttt agtgatatag tttttttata tgtttagctt123720agatccagaa gataatgagc tacatttgtc ctctttaagc tgactatatt atttattctc123780
tttgtatcat cagttatgtt ttaggaattt tttttaaaaa caaaaccaca ttgaggtgct123840gtaattattc tttttcttta aaggtttcga ccaaatacta gagagctgtg gtgttacaat123900gcggtggttg ctgatgccag gcttccctct gcagcagaca tgcagtccag atgtagtatc123960ctaagtcctg aacttgcctt accaacagga tcaagggccc tcactacccg atctcatgca124020gctttgcaca ttttaggtag ggttgcgatt tgatgtacca ttaattcaca ttaatgtgtg124080ctgtccaggg ttttttttta gagtatttaa atttatatgt aattctgtct tgtttgttaa124140ataataataa tgtacaaaat aagttaaatc ctctaagagg ctaattccac agaaaacaat124200acatgagacc tgtactaaca ctatcaaaga tttatgtatg cccaattatt taattttcac124260agaggtagac ccttaaaaat aatgtttttg ctcaacttga atgtataaac ttttaaagat124320atgataaaat tttcaagtat ataatattta atttatgtat attatgttaa ataaattcta124380taaaatgtaa tgaattttat atacatcttc tgtttcttct tatataacac agttttccac124440agcatatcaa cttcattctt atttaattac tttgagatgt agtacttatt taattacttt124500gagatgtagt gcaatatatt attttgtaac tggaaatttt atatcactct atgtaactat124560gtattttgtt gcttttaaaa atgatcctta aaaagactca ttttcagact ctagcacttg124620caggtgtgaa atcatattcc taaatataat taccatatca taaatataac tctttgtttc124680ttttttctca aatttcattt tggtttctat tgtagacatc caaaactagt gttgattaag124740tttgtgtgac actaatgtgt cttcctcaaa tagcacttta agaatcaaac taatttggag124800tttcataaaa ggaagaacct tgtaagaatt caaaggttaa gtgatttcaa cttttcagag124860atccagtttt gtgtaaaagg ttgtcgtatg gcaagtttaa atatgatcat taatcagaca124920agggataatt tgtattggtt ttaagcattc ttccatgaaa tgatgtttaa agcttggagt124980aacattctga gtttatttat tttaatttat cccctatagc atttaatgtc atcaatacca125040taggacattt taatttcaag gagttaaatt ttgtttcttt gttgttttag gttgtcttga125100taccttggca gctatgcagg acttaaaaat gggtgttgca agtacagagg aagagactca125160agcagtaatg aaggtttatt ctaaagaaga ttatagtgtg gtaaacaggt ttgaaagtat125220gtatacttcg gtttaggaaa tgttgtctta caactgaaat atatatggat cttttaaaac125280atagattatg atttatatat tctaggtcat ggaggaggct ggggttattc tgcccattca125340gtagaagcta tacgtttcag tgccgacact gatattttac ttggtggtct tggtctgttt125400ggaggtagag gagaatatac tgctaaaatt aaggtaaagt tcatcaacaa tgttgtcctt125460
ttttgtttga acatactgat tttgacttaa tttattattt tattatttaa tgtctaattt125520ttttcctttt taactgggtt tattttgttt ttaatgcttc tttttaaaaa gcgatagaga125580aaacattgct aaaatcgaat acagtaatac taaatttttt aaatttattt atttattatt125640attatacttt aagttttagg gtacatgtgc acaatgtgca ggttagttac atatgtatac125700atgtgccatg ctgatgcgct gcacccacga acttgtcatc tagcattagg tatatctccc125760aatgctatcc ctcccccctc cccccacccc acaacagtct ccagagtgtg atgttcccct125820tcctgtgtcc atgtgttctc attgttcaat tcccacctat gagtgagaat atgcggtgtt125880tggttttttg ttcttgcgat agtttactga gaatgatgat ttccaatttc atccatgtcc125940ctacaaagga catgaactca tcatttttta tggctgcata gtattccatg gtgtatatgt126000gccacatttt cttaatccag tctatcattg ttggacattt gggttggttt caagtctttg126060ctattgtgaa taatgccgca ataaacatac gtgtgcatgt gtctttatag cagcatgatt126120tatagtcctt tgggtatata cccagtaatg ggatggctgg gtcaaatggt atttctagtt126180ctagatccct gaggaatcgc cacactgact tccacaatgg ttgaactagt ttacagtccc126240accaacagtg taaaagtgtt cctatttctc cacatcctct ccagcacctg ttgtttcctg126300actttttaat gattgccatt ctaactggtg tgagatggta tctcattgtg gttttgattt126360gcatttctct gatggccagt gatggtgagc attttttcaa gtgttttttg gctgcataaa126420tgtcttcttt tgagaagtgt ctgttcatgt ccttcaccca ctttttgatg gggttgtttg126480ttttttttct tgtaaattgg tttgagttca ttgtagattc tggatattag ccctttgtca126540gatgagtagg ttgcaaaaat tttctcccat tttgtaggtt gcctgttcac tctgatggta126600gtttcttttg ctgtgcagaa gctctttagt ttaattagat cccatttgtc aattttgtct126660tttgttgcca ttgcttttgg tgttttagac aagaagtcct tgcccatgcc tatgtcctga126720atggtaatgc ctaggttttc ttctagggtt tttatggttt taggtctaac gtttaagtct126780ttaatccatc ttgaattgat ttttgtgtaa ggtgtaagga agggatccag tttcagcttt126840ctacatatgg ctagccagtt ttcccagcac catttattaa atagggaatc ctttccccat126900ttcttgtttt tctcaggttt gtcaaagatc agatagttgt agatatgtgg cattatttct126960gagggctctg ttccgttcca ttgatctata tctctgtttt ggtaccaata ccatgctgtt127020ttggttactg tagccttgta gtatagtttg aagtcaggta gtgtgatgcc tccagctttg127080ttcttttggc ttaggattga cttggtcttg cgggctcttt tttggttcca tatgaacttt127140
aaagtagttt tttccaattc tgtgaagaaa gtcattggta gcttgatggg gatggcattg127200aatctgtaaa ttaccttggg cagtatggcc attttcacga tattgattct tcctacccat127260gagcatggga tgttcttcca tttgtttgta tcctctttta tttccttgag cagtggtttg127320tagttctcct tgaagaggtc cttcccatcc cttgtaagtt ggattcctag gtattttatt127380ttctttgaag caattgtgaa tgggagttca ctcatgattt ggctctctgt ttgtctgttg127440ttggtgtata agaatgctta tgatttttgt acattgattt tgtatcctga gactttgctg127500aagttgttta tcagcttaag gaggttttgg gctgagatga tggggttttc tagatataca127560atcatgtcgt ctgcaaacag ggacaatttg acttcctctt ttcctaattg aatacccttt127620atttccttct cctgcctaat tgccctggcc agaacttcca acactatgtt gaataggagt127680ggtgagagag ggcatccctg tcttgtgcca gttttcaaag ggaatgcttc cagtttttgc127740ccattcagta tgatactggc tgtgggtttg tcatagatag ctcttattat tttgaaatat127800gtcccatgaa tacctaattt attgagagtt tttagcatga agggttgttg aattttgtca127860aaggcctttt ctgcatctgt tgagataatc ctgtggtttt tgtctttggt tctgtttata127920tgctggatta catttattga tttgcatata ttgaaccatc cttgcatccc agggatgaag127980cccacttgat catggtggat aagctttttg atgtgctgct ggattcgttt tgccagtatt128040ttattgagga tttttgcatc aatgttcatc aaggatattg gtctaaaatt ctcttttttg128100gttgtgtctc tgcccggctt tggtatcagg atgatgctgg cctcataaaa tgagttaggg128160aggattccct ctttttctat tgattggaat agtttcagaa ggaatggtac cagttcctcc128220ttgtatctct ggtagaattc ggctgtgaat ccatctggtc ctggactctt tttggttggt128280aagctgttga ttattgccac aatttcagat cctgttattg gtctattcag agattcaact128340tcttcctggt ttagtcttgg gagagtgtat gtgtccagga atcccttatc catttcttct128400agattttcta gtttatttgc gtagaggatt ttgtagtatt ctctgatggt agtttgtatt128460tctgtgggat cggtgatgat atccccttta tcatttttta ttgcgtctat ttgattcttc128520tctctttttt tctttattag tcttgttagc ggtctatcaa ttttgttgat cctttcaaaa128580aaccagctcc tggattcatt aattttttga agggtttttt gtgtctctat ttccttcagt128640tctctgattt tagttatttc ttgccttctg ctagcttttg aatgtgtttg ctcttgcttt128700tctagttcct ttaattatga tgttagggtg tcaattttgg atctttcctg cttcctcttg128760tgggcattta gtgctataaa tttccctcta cacactgctt tgaatgtgtc ccagagattc128820
tggtatgttg cgtctttgtt ctcgttggtt tcaaagaaca tctttatttc tgccttcatt128880tcattgtgta cccagtagtc attcaggagc aggttgttca gtttccatgt agttgagtgg128940ttttgagtga gattcttaat cctgagttct agtttgattg cactgtggtc tgagagacag129000tttgttataa tttctgttct tttacatttg ctgaggagag ctttacttcc aagtatgtgg129060tcaattttgg aataggtgtg gtgtggtgct gaaaaaaatg tatattctgt tgatttgggg129120tggagagttc tgtagatgtc tattaggtcc gcttggtgca gagctgagtt caattcctgg129180atatccttgt tgactttctg tcttgttgat ctgtctgatg ttgacagtgg ggtgttaaag129240tctcccttta ttaatgtgtg ggagtctaag tctctttgta ggtcactcag gacttgcttt129300atgaatctgg gtgctcctgt attgggtgca tatatattta ggatagttag ctcttcttgt129360tgaattgatc cctttaccat tatgtaatgg ccttctttgt ctcttttgat ctttgttggt129420ttaaagtctg ttttatcaga gactaggatt gcaacccctg cctttttttg ttttccattt129480gcttggtaga tcttcctcca tccttttatt ttgagcctat atgcgtctct gcacgtgaga129540tgggtttcct gaacacagca cactgatggg tcttgactct ttacccaatg tgccagtctg129600tgtcttttaa ttggagcatt tagtccattt acatttaaag ttaatattgt tatgtgtgaa129660tttggtcctg tcattatgat gttagctggt tattttgctc gttaattgat gcagtttctt129720cctagtctcg atggtcttta cattttggca tgattttgca gtggctggta ccggttgttc129780ctttccatgt ttagcacttc cttcaggagc tcttttaggt caggcctggt ggtgacaaaa129840tctctcagca tttgcttgtc tgtaaagtat tttatttctc cttcacttat gaagcttagt129900ttggctggat atgaaattct gggttggaaa ttcttttctt aaagaatgtt gaatattggc129960ccccactctc ttctggcttg tagagtttct gctgagagat ccgctgttag tctgatgggc130020ttccctttga gggtaacccg acctttctct ctggctgccc ttaacatttt ttccttcatt130080tcaactttgg taaatctgac aattatgtgc cttggagttg ctcttctcaa ggagtatctt130140tgtggcgttc tctgtatttc ctgaatctga atgttggcct gccttgctag attggggaag130200ttctcctgga taatatcctg cagagtgttt tccaacttgg ttccattctc cccatcactt130260tcaggtacac caatcagacg tagatttggt cttttcacat agtcccatat ttcttggagg130320ctttgttcgt ttctttttat tcttttttct ctaaacttcc cttctcgctt cacttcattt130380atttcatctt ccatcgctga taccctttct tccagttgat ctcatcggct cctgaggctt130440ctgcattctt cacgtagttc tcgagccttg gttttcatct ccatcagctc ctttaagcac130500
ttctctgtat tggttattct agttatacat tcttctaaat ttttttcaaa gttttcaact130560tctttgcctt tggtttgaat gtcctcccgt agctcagagt aatttgatca tctgaagcct130620tcttctctca gctcgtcaaa gtcattctcc atccagcttt gttccgttgc tggtgaggaa130680ctgtgtttct ttggaggagg agagttgctc tgctttttag agtttccagt ttttctgctc130740tgttttttcc ccatctttgt ggttttatcg acttttggtc tttgatgatg gtgatgtaca130800gatgggtttt tggtgtggat gtcctttctg tttgttagtt ttccttttaa cagacaggac130860cctcagctgc aggtctgttg gagtaccagg cagtgtgagg tgtcagtctg cccctgctgt130920ggggtgcctc ccagttaggc tgctggaggg tcaggggtca gggacccact tgaggaggca130980gtctgccctt tctcagatct ccagctgtgt gctgggagaa ccactgctct cttcaaagct131040gtcagacagg gacatttaag tctgcagagg ttactgctgt ctttttgttt gtctgtgccc131100tgcccccaga ggtggagcct acagaggcag gcaggcctcc ttgagctgtg gtgggctcca131160cccagttgga gcttcttggc tgctttgttt acctaagcaa gcctgggcaa tggcgggcgc131220ccctccccca gcctcgctgc cgccttgcag tttgatctca gactgctgtg ctagcaatca131280gcgagactcc atgagcgtag gaccctccga gtcaggtgca ggatataatc tcctggtgtg131340ccgtttttta agcccgtcag aaaagtgcag tattcaggtg ggagtgaccc gattttccag131400gtgccgtctg tcaccccttt ctttgactag gaaagggaac tccctgaccc cttgcgcttc131460ccgagtgagg caatgcctcg ccctgcttca gctcgcgcat ggtgcgcaca cccactgtcc131520tgctcccact gtctggcact ccctagtgag atgaacccgg tacctcagat ggaaatgcag131580aaatcacccg tcttctgtgt tgctcacgct gggagctgta gaccggagct gttcctattc131640agccatcttg cctcctcccc ccctacagta atactaaatt aataactcat taaaaaaatc131700aaaattctgt aagaagggaa gaaatgaaag gaaaattatt ttcaacaatt taccactgaa131760gaaagactat atctcagtcc tttctgtgga acttctggtg gcctagcaat accagtgcct131820ttttatgtgg ctactttctt atagccattt taatgacaga ggaggcacaa ctgtttaggg131880acattttgat gtagtatgta aacagacatc ttcaccccag gagtggagac cgggaggagt131940gagagatagt gttaggggag gattccatct ggtaggccaa ctaaaaatca ttgagagtca132000tatgttacca cacctattcg tatccaaaag ggccaacaaa ttatcctaaa caaagtatat132060gttcattctt tgaatattca ctaatagctt gaatgttctg cttctcaaat tacctgattt132120tgctctaaaa gtgaaagaga aaactatagt ttctgcctga atttcaatag caggcttcac132180
aaaaaaatct cgattaaatg tcatctgttc aggaggtatt ttcttagttc tctcaataat132240atctaaaaat gatatcaata tgttaacact cttcttcata atgctggttt ccaaggacat132300tccccatgga ggcccaacca gaactgacaa acttaaagaa atatttgctt atttctggtt132360atagttggaa tacatgctaa aaaagcagat aatgtaagaa tgaataaagt aaaaatttaa132420agctcctata gttatatgga gtatagtgat agaataatga ttaggactct tgtagcacat132480atattttgat aataagaaaa atagtagttt ggctcatttc agtgcaactg ctttaaaatg132540gttattttag gatgtcttga ttcatcctca cgttagagaa gcaacctcaa tgtgttatgt132600agttctattt gacaatgtct aataagctca tcaggaggat attttttttt ctagctctaa132660gagatttttt cttttcggaa tagtgaacaa cgtagtggac cttatcttca aacactgtag132720tatatagaat tatggaggcc ttccatatat gttgttttta tattggccct atttttcttt132780ttcaaactca aattttacct cactcctcct attgtctgga aatgatcttg ccttttgtat132840tcaattatcc agtgagtggg ctaaggcctt tcaacagtag tcagtctttt tttttccttc132900ccttcacctc tctgtaatgt atgcatttgt taagcccttc tgtatttttt tgttttgttt132960tgttttcata cctgtagttt tttcttctat ctctttgtag gttcatcccc ttccacctgg133020ccactgaatg taggagttcc tcaaagaccc agccctaggc cctctttctt cacatattcg133080tatatgcatt ctcttttgag cccagagttt taatagccat cagcatacca taatccccaa133140atttgtatct ccaactcaga cttctttgat gcattttcat gtaaatatgt taaaggcaca133200tcagttatca gtcataatat ctcaactgaa ttcatgctct tccactgatc tctctctctc133260atgattagta ccatccacca tatgcttgat acataggccg gaaatctaga catttttctt133320gataggcctc ctatatctct ttcttctcca caaaaaagac ttttctacca ctaccctagg133380caaaggtatt tgtcttggac tagccaccta gatagtctct gtacatattc tttctatact133440ttctgtctcc attctctctc ttaccattcc tacttgagcc tactccagtc agatttttgc133500ccccaccact gacattgctc ttgtcaagga cactgatgac tttcatattg ctaaattcaa133560tagtcaagcc tcagctctca tcttccttaa ccagtcagct gaatttaacc caattgagct133620cttctttgaa atactttctt ctcttgtccc ccacgacact acacttcatt tttcttttgt133680cccactgact gttcttctta gtctcctgct ggttgctcat catcttcctg acttctaaat133740gttggagtgc caagaactca gtttttgaca tcttttaaaa gtattctctt cctcatagtg133800gtccagcctc atggacattt atctctgatg acttttaaat ttataaacct cttctctgac133860
ctttaaacca atatgtacaa cagcctacgc tgtgtctcca cgtgactctc tgataggcat133920tttggaggta acatattcaa tactatgctt ctgatgatct gcctaaacct gctatcctca133980tgttcttccc catcccagtt aactgactca gcatggagtt aacttcattc ttctgtttcc134040tcaggccaaa aatcttggag tcatccttga ttctactctt tcttttacac acatccactt134100cgggagcaaa tattatcgtt tccactttca aaataaatcc cgtatctaac cacttctgaa134160aactgccact gctatcaggt tgggtcatgc cacagccatt ctcatttgta ttattacagt134220agcctcccaa cttgtgttct acgtctgtcc tcaccccatt tgttctgttc tgagtgtggc134280agtgattctc ttaaaatgta caacagattg catcattgtc tcttcagaat cctgtagtgt134340cttcctatgt gagaatgaga accaaaagcc ttagaaggac ctacaaagtc cccatcagaa134400ctggctgtca tcacctctct gaccttttgt tctactagtt gtttcactct aatccagtga134460cactggcctg cttattgttc ctcagacatt ccaggcacag tcaaatctca gtacctcttc134520acttgctgtt tcctcataac tacagctctc ttcacttcag cacctacata gcccacttcc134580ccatcacctt cagagcttca ttccctggta agcctactta aaactgtaaa ttcaattccc134640aaccatagac ttactcccaa tagcccccaa ttctttgatt gtattgtttg cccctttatg134700cccaatttct agaactgtgc ttggcataca gtatgtgttc aaaaatattg gttgaatgta134760aatacaggag tcagattggg gacaaattat taagaacctt catatcatgc tgtggaggag134820atgagaaacc aaggaataat tttagattaa gaaagtattc tgataagata tttttataag134880ggtactaagg gtcatgaaac aaatacaaaa atcctcaact ttgtggaata tgggttagaa134940gaaggagaga atggagataa gactaattag ttgttgaaat attccagcca gcaataggga135000atatggttga attgacaagc tttggtacca gtaagagaat agatacagta atggcagaga135060aatatggaaa aatgattatt tcggactatt tttttttaag agtctctttg gtagcccaca135120ctggagtgca gtgatgccat cttggctctc tgcagcctct gcctcctggg ttcaagcaat135180tcttctgcct cagcttcccg agtagctggg actacaggca tgtgccacca cgcccagcta135240atttttgtat ttttagtaga gacggggttt caccatgttg accaggctgg tgtcaaactc135300gtgacctcat atgatctgcc tgcctcagcc tcccaaagtg ctaggattac aggtgtgagc135360caccaggcct ggcctatttc agacttttta tctaagagat taggtgaatg aaagaccatt135420aaccagaaca gagaaggcag gaagaaggta tagaactagt aggacaaagg gaatccaatg135480actgttgaga ataagagctg agctggagaa agatttctaa gtcattagca tataatttaa135540
aaatttagac acctagcaca gtacttgtcc agtaaatttt aatgcatttt aatctttatc135600tgcctctctt gcaattcacc ttccataatt atccagagga aaaaggtttt cttaaaaaaa135660aaaaggacat tttttaaaaa gacctcttct tcataattct tattttcact taatttcata135720acctttttgt attggatatc ttttctgttt ttttcttacc cagaggatct tcccctcttt135780tttaagagcc atttgtttca tgcttttcat ctatcttttt ctcatgccat attactttga135840actatatttt gttacctatt gtttttaagg attcttatgt tgctcttctc tctctgtagc135900taatatttct ctaccctttt ttattgtttg tttttttaag aattctacag tattcactta135960ctttttaaaa gtactgtgag atgggaactg ttagtatcca cccacctttt atggatgagg136020aaactaggtc ccaggatctc agttagtaag tgatgaagca acattctacc tcatttagaa136080tgaggtagaa tctcacttag tgagagactc acttagttaa aattttttgt tggagttctc136140tcaccttttt cagatgcaca tattaggtac aaaacttgta tagaactatt tagagcattt136200gatgtaaata gatgaatata taaattagga aaacagagat taatgtagac cataaaacct136260tgtttcctaa tttaaataca tatgtttgtg tatttcacat agtaatgtta aaaaagcagg136320tactctgatt ttttacattt ggtatggtcc agcatacatt catcatgcac aaaattatga136380acacattgtt catcagatat gtatagctga tttatataaa tgattgtctt tctcctttcc136440ttagctgttt gaattgggtc ctgatggagg agatcatgaa actgatggtg accttcttgc136500agagactgat gtattggctt atgactgtgc tgctaggtaa ataatttgtg cattattcat136560tgtaactctt gcatacttaa tattaaaaat ttattataaa tgaataaaca tactttaaaa136620ggaagtttta aatctgctat gaatatcaaa tacttgagaa attaaattat atattaagca136680atataagtat tgctttcaaa aatgctgaat taaaactaat atacttcata aatttatttc136740ataagtcaac tgtttcaata ttgaagataa aaatggaaat atgcatatat tttatacacc136800tttactttca tgcatctctt acggaatacc tagtttgctg gtgttctagt aataatacag136860ttatttatta ctcctagcat tacaggtaat aaataattag cgtcaagtgg tcatgtttga136920ccataattca gattacccat gtactctgac atcatgctgc tagaatatat ttgataataa136980cacacttggg gaggtgacta gctttactga aaccttttgg gagtttataa aaattagtat137040gtttatttat gaaggttcat taagtaagag gatacttaca ctttatcagt cctctctacg137100taatcctaag aattatctca atattgtgtt atgatcagtg ttccctggtt tgaagcaggg137160aatattatta taagaaaaat tataaactga gcttcatagt agtaatagtg tgctatgttt137220
cccaacaata actatgttag gaaaaccttg taggttttct tgctagtgaa aatgtatatt137280ttcagaaaca aggatgatcg taagatactg actttgcttg tacaagatat aaatactttt137340gaaaacaaaa gagtaaatct aaagaaaatt ttgactattt cttaggcatt aactaatatc137400gcaagatttt tttagtaacc tttagtgttt tatacaaatg aatattaggg aaaattttaa137460gaaagctaat tgattaattt tcagcaaagc cttaaggtgc cattgtcatt tgccgtattt137520atttatttct ttatttattc atttattaat ttttattttt gagacagagt ctcactccat137580cacctaggct ggagtgcagt ggtgtgatct caactcactg caacctctgc ctcccggatt137640taagcgattc tcatgcctca gcctcctgag tagttgggac tacagtcgca tgccaccagg137700cccggctaat gtttgtattt ttagtagaga tggggtttta ccatgctgac caggctggcc137760ttgaactcct gacctcaagt gatcctcctg ccttggcctc tgaaagtgct gggattacag137820gaatgagcca ctgtgcctgg ctgccatttg tttttgacca tatgagtttg agtcgaaaat137880ataaagcaca gtttcattta tagagaatcg tctaggattt cttcattagc ttatatacaa137940agcccagact cctaaggcat tgggagcatt gaaggatttc cataacatcc agcatctttt138000ttgactttct accactactc ttctacagct gtcttaatgc attaatgaaa ttaataatga138060acaccttgtc atttcctgac ctcttttgta cttccatacc tctacagatt ttttcatgac138120attctcctgc ttctttaaat gccctttatt cttctttcaa ttccagctac aaccaataaa138180cattccttcc actctgttac ctagtacttt gtaacttgtt tatatccttt agaagatgcc138240cttatttatt gtcttttttc ttcttctaaa ttaataatgt ttgtatagat attatgtgac138300agactccaaa cacctatctg acttaattca ttctaaagga caactctatg agcaggatat138360tattttcacc acagttttca ggtgaagaag ctaaggtact gagaggatag agtaacttgc138420cctgcgttgc cctgctagca ggtcacagag ctgggccttg aacccaggct gcctgactcc138480agggccattt tcttaatatg ctgcctttca aaactattta gctgaatgaa tgagctttaa138540aactaaagtt cagattttta ttttccagca aaatttgaat gttttttaat atcagtttaa138600aacattctaa ttttaatatg aaagttttat aacatttaag gatattgtag aattcaagaa138660gaggaggaag aaatgtaatt ctatcaccat aacataaata actatatttg cttgcattct138720tgtccagact tcttagagtg tttttaatgt tgtttgataa gaattttata atacagttag138780tttcatgttt ttgttcacaa gggattttga aaaaataaat atatgtgaca gagaagtaag138840aaaatattgt ttttgactca ctaatttttt ctcagagaaa aatatgcaat gatgtttgat138900
gagcctgttc tcctgcaagc tgggtggtgg tatgtggcat gggcccgagt gtcaggaccc138960agcagtgact gtggatctca tggacaggca tctattacca cagatgatgg gtaagtaaat139020gcccaagtgt tacttaagca atacttattt ttgttagagg aatattgttt tgaaaattca139080gagtactttc agcaggattc aaaagcttag gaagactaga gcataatata actgctaaaa139140attaaattca tgtaacctca tgccaaattg aggtctagac agaacaagga gataataaga139200ccactgtact ttgctcctgt cagatggtat ttgtatatat ttctgggtat tacattgttt139260acgagcttga ttaccaaatt agaaattata tagaggaagg tgacaaggaa aatgaggagt139320ttggaaccat aaaaaagaag tagatatgtg atattaaatt tgatagtgca tgagaaaaca139380gtttaaaatt tcaaagcttc agcaatgtgt gttattttaa taaaatatag ggaaaatttt139440gctgttttaa aataactttc acgtatagag tacttttcag ttcatattct tatattttat139500ctgatttgat gccctcaata gctttgtgaa gcagttatta gccccatttt atactccagg139560gactagagaa ataaaaaggt gaaattattt gctatttcct gagagtcatg tggttaaaac139620caaggtcaga cctgtaacct gttgactcta aatccaacat ttcaatattc tattactatg139680atttatgtat tctaaaggac tatcatttgt aagataaatt cagtttattc ttgattactc139740ctgagcataa actatttgaa atcagttgaa gttatataac agtatatatt ccagtgcagg139800tcaattcata acaaagctga ccaaaaaaag aaatagactt atttgtgagg tgaatttcct139860gttgctgaat attcaaggag actactgtag aaaaatcaca tctgttgtta tgtgcatcaa139920cttaagaatg tctttagaac taagtgacta ctttgaccct ggactgtatg attttcatag139980acagttcatt cagacttgga atgtcatggc acactgaggc taaagcttat catgcagcta140040gtcaagcaga tattatacta cttagttgta agtagaaatt ttagaagttt actgctgtgg140100ttaaagctga gttggagagc taggagggac aggacagcta ctcatttagg agtcctaatc140160acgttcagtt ctttcatttt actgactaac ccattcagag ctagaatgaa aatctagaaa140220ataccttttt gcttttcttt atgtaaaaga gtgaatcatt ttcattgaca taagatttga140280aaacatgttt cttaaatttc ttatttcctg taatgatcat gttgtttttg gtatcattgt140340tttctcttct tagggttgtt ttccagttca agagttcaaa gaaatcaaat aatggtacag140400atgttaatgc gggtcagata cctcagttat tatacaggta tttagcatat ttatactgaa140460ttagtatggt ttattatttt gctataatga agtagaatca ttacaaaacg tggcataata140520gggagaaatt atttgaagca ttcagcaaat tataatatgc tggtagaact ataaatatat140580
ttaacaaaaa cagttaatat tttgtatatt tttaaacatt ttccatacag gcttaacttt140640aaactttgcc tgattactca gattatctgt attataccaa taacaatatt ttactgactt140700taaaaaaaat ctatccattg attttcttac taatttcaaa tgttgtaaaa gaataaaatt140760tgtcttggag aagtgagatt gcagggaaga tggggaggac tatagatgca ggcctggttg140820atatattgta gtctcataag actgataaaa tgagttggaa aattccctac tgttggtaaa140880ttttcacctt tagctaaaat accaaataat aacttgctca tataatattt actatatttt140940taacattatt gaaaaatttt aacttacaat aatttaagca tgtataaagg catatgcctg141000tgtttacctt atttatttct agactactgc aatactttta gaataaagaa ttcaaatgga141060tataaaccgt tactgaaaaa tgttgtcatt cgttaagcag taaaaaatca aaggaaatgg141120tctagaactg tgattctatc tccctatctt tctttctggt aatcactttg tggttacaaa141180cattattttt ccccaaaagg ttttttcgtt tgtttggttt ttatgcaccg tgcaatctgt141240tatagttact gtttgtaata tgtagctcta caaacataac gtgttgcatt ggactactcc141300caggtactca ctggttgaat aacttttaaa taacagcatt aatagtagca ttactagaga141360atataagtta tatgttatat acattatata tgttctctaa gaagaggaaa aagcctctac141420tactcggtct tgcgaaggta ggatttaaag gaagtgccca tatgctaaaa actttgttta141480gcttggaaaa atgtttcaaa aaagccctgt attggcatta cctggcaaaa aaatttaaat141540atataattta ttttcccatt caaaaatgta taatatactt taaaaagaaa aaatgacatt141600ggactttcta acataatttt atatgtgtct actgccttta gacttccaac cagtgatggc141660agtgcttcaa aaggcaaaca gcaaaccagt gaacctgtac acattttaaa gaggtctttt141720gcaagaactg tctcagtggt aagccatgtt ttaaaaaatt tcagcttttc tcttcagtcc141780tcagatacat tttgatgttt agagtttagc tgctcttgta agtttatctt agcctagatt141840tataaactca gatttggttt tgtattcaca tttatattct agaccacaga taaagtatac141900tttaaatcat agaaaaatgg aggtgttggc ctatgatttt aacatgacca gatgaattgg141960ccttgctgtt tttgaaacaa ataacgattt gcttttctag taaatcatat atgaatgcat142020atgctgaatg aattagaaaa tgaaggaaaa gttaaggatg gtgctggccc aaaaaagcgt142080gattactact tgaggaacta ggtggaagag ttgcatatct aactaggaag tttgttgaac142140cacttgccat ccccattgta cacatgaaga agcacagtgc cattgaggtt aacagtgcag142200aatggaaagt caacatgatg taaataaatg aaagtataaa ttaaggtctt gatctctcat142260
gtattaattg aggattctta cgtatgttac ctaagtaaat tacatcatag cattttgaat142320ttctaatgag ataaggggtg taagatgttt ttggaactgt aaggcaccat ctgaatgtga142380tttattaata tagctagtag tctttcatat tagtaaaatg cataggaaga atgtttcttt142440cccttttaaa tttatttttc aacccaccaa gtgaatatat attcctggta ggaatgagat142500aaaagtatcc taaaagtata tgaaagattt ttctcccatt ctcatgatca ccatatatac142560ctactattta cattttggtg tgtatcttcc cagacctttt tttttttttt tttttggtga142620ggcagagtct cgctctgtcg cccaggctgg agtgcagtgg cacgatcttg gctcactgca142680acctccgcgt cctgagttca agcaattctc ctgcctcagc ctcctgagta gctgggatta142740caggcgcctg ccaccacgcc cagctaattt ttgtattttt agtagagagg gggtttcacc142800atgttggtgg tggtctcgaa cccctgacct cgaacaggct ggtctcgaac ccctgacctc142860atgacccacc cgcctcggcc tcccaaagtg ctgggattac aggtgtgagc caccgcaccc142920ggccttccca gaccattttt atatgcaaaa aaacttatta gtacgtatca aatacaggat142980gctctacaac atgcttgctt gtttcttacg atacttacag ttttcatctt ttttagaaaa143040tttcgtatta tatatcatgg acatccttcc ctggcagtac atttggctct gtcagtcttc143100tccgtttgcg taccattatt ctaatttatt caatctgata tctatcaaag aaccactttg143160attctcattt tttgttattt catcttgctt catgagcatc tatacacata catctttaaa143220tacttgtgta cttatgtgca tacttctact ggaaatatgc ccatttaaag tttggtatat143280cctctcaaag ttgaccttcc aaaagagcca tggcagtttg tagagcttgt gctttgaata143340aaagactatg agaactttgg cttcttggta actttgcatc ctattagtct taagttgagt143400ttggtatctt ttgattcaaa taaggatctt tgactctgtt atgctttctt tagtgatctc143460acttttctgt gcttggtaac ccctttgtat ttatgtttaa gtggttttgt ggccattagg143520taattgtaga ctttatatac aaaacttatt cagtcatttc tttaaaaggt aaactatagg143580gcttagcaca ttggctcacg cctataatcc cagcactttt ggaggccgag gcaggaggat143640tgcttgagct caggagttca agaccagcca gggcaatata gtgaaacccc catctaaaaa143700aacttaaaaa ttagctgggt atggtggtgc gcacttgtag tcccagctgc ttgggaggct143760gagggggtgg tgagaggata gcttgagtcc aggaggttga ggttgcagtg acccgtgatc143820acaccattgc attccagtct gggtgataga ggaagactct atctcaaaaa aaaaaaaaaa143880aaaaaattga atctataaac tcgttttcag gagctctggg atttggtggg aatggggcag143940
taagtgctct gctttgagat tcaggtggta tctgttgtca tttaaccatg ataaaaccag144000aagactagaa ctgcaaagaa aatctaccat cttaaactca tatgctagaa ttttaaataa144060tcaaaatagg aattttttag tattttctag tcaattttta tcttctatta atgtatttat144120caaaaattga ctgtagtttg tattgcaaat tttaatatat agacataaaa taagttattt144180gatactgtta ctgatatcta acactaaaac tctattttat tttttactac agtgactgta144240aaaagcttta tttttctttc aagtaaacat gcattgtttt ataagtgctt ctcatttttc144300ccttgtttat atgataagct agatagctgg gtagcaggaa cactgaaaaa tttattttta144360aataggtaaa tattgaagaa tgttgttttt agaaatgctt aactacttta ccttttttgt144420aacaaacttc aaagttactg ttgagggtaa tttaataaat gaactataaa agtgtcttgg144480gctcagtaaa aaaaattcaa ttaaaaaact cagtaaaaaa attcaaaaga aagttttgaa144540gttgtctacc attgcctttc catttgattc cgtttatggt atcatttgaa ttaattgttg144600tctgtttttt catattctgg attaggaatg ttttgagtca ttgttgagta ttcttcactg144660gagctggacc accttagtct taggagttga agaacttaga ggattaaaag gattccagtt144720cacagctaca ctcctagatt tagagagact gcgctttgtg ggtacctgtt gtctgaggtt144780attgcgtgtc tatacctgtg aaatttaccc agtgtcaggt atgatgcatt ttttttaaat144840gactataggg agaaaatatt agagacattg agaagattta ctttgagata actgagagtt144900tatgcccccc agctgcattt aagctgcttc taaaatgagg ggagaaagtc agcttgctat144960gatgcttatg tatttgctta taaagagtaa aatagattac atgtcattct gatacaaaaa145020gtaatagtgg gccaaagtta aataatctaa taaagtttaa ttttaaacaa attctgtcaa145080atagaaatat tttgaacaag caatttggac ttaaatgctg aaactaggtt tttattcccc145140cttccattta gattaaaaat tgggactaca cttaatctct aagttgtttc atttctaaga145200tattagtact tgtgatactt agctgtatag ggaattactg aattatattg attgttttca145260tgcatattta atattttgct taattaccaa tctgcttttt ccccctcaaa gctacaggaa145320aagcagttgt agaagaaact agcaaattag cagagtgtat tggaaaaacc agaactttgt145380taagaaaaat tttatcagaa ggagttgatc actgcatggt gaaattggat aatgatcctc145440aaggatatct cagtcaaccc ttgagtcttc tagaagctgt ccttcaggaa tgtcataata145500ctttcactgc ctgctttcat tctttctacc caactcctgc cttacagtgg gcttgccttt145560gtgatctgct gaattgtttg gatcaggtaa tttaagtttg taaaatgtta gttgaaaatc145620
tgatatattg cttaatactc ttcaagaaaa taaattgcag ttgccatatc ttcttaactt145680gtataaaaga taaattagga aatcattcgg tgtacatgac ttatataagc aaagtttaaa145740attttttaaa tctgcttcat catcttttag ttacaaaatt ggtaggcaca ttgtgcagtt145800gctgattggt ttttgaattg gagtgagtga ttctgagaga gggagagagg aaaagaacca145860tagtgaccag gggagagcag ggatagcacc aggaggcccc cgccaccctc atctgtgaga145920acaggaagga gagagagctg agagaacagt catactagca ttgttcattc attcatgagg145980aagccacaac ctttgtaaac ctttatgcac caagtcacag agtttcacaa cacctgaggc146040aaaacactga cagaactaca gggagaaata gattcacatt gacagctggg gactttaaca146100ctcctctttc agtaattaat agaggaacta ggccaaaaac atcagcgatg cagatttgaa146160cagtactgtc aaccatcttg atctgacatt tacaaaacag gatatgaaac tgcaaaatac146220atattctttt caagtacaca tgatttttaa aagaaatatt tgggaccatt cactaaggta146280gatcatatgc tagaccataa aatgtcttaa taaatttcaa aagattaaaa tgtgaaagaa146340tatattttca gaccacagta gaattacagt aaaaatcaat agcaaaggat atccagaaaa146400agcaccaaac atttggaaaa tgacagcact tctaagtaag cagttgatca aagaagaagt146460tataaaggaa attttaaaat gtttcacata gaaagataaa aacataatat cagaatttgt146520gggatttagc taaagcattg cttaaaagag ctctttatac ccttaaatgc ttatatgaag146580aagaaaggtt taaaatcagt gacctaagct gctatgttaa gaatctagaa ggaaaagcaa146640atctaaccca aagttagaag gaaggaagga aataataaag ataagagcag aaatcaatga146700aatagaaagt agagaaaatt aacacagcta aaagttggtt ttttgaagaa aaaaagttgg146760taaactccta gcaaacctga ctgggagaga agagagaaag aaaaaacatg aaataccata146820atcaaaagtg aaataggggt tcaccgggca cggtggctca tgcctgtaat cccagcactt146880tgggaggctg aggtgggtgg atcacttgag gtcaggagtt cgagaccagc ctaacatggc146940aaaactcagt ctctaccaaa aatacaaaaa ttagccaggc atggtggtgc acagctgtaa147000tctcagctac tcgggaggct gaggcacgag aattgcttga aggcgggagg tgaaggttgc147060attgaggcaa gattgtgcca ctgcactcca gcctggtgac agaatgatac tccatctcaa147120aaaaaaaaaa aaaaaaaaaa aaaaaaagaa atagggacta tcactataaa caactttata147180ctggtgaatt tttcagcctt agaggaagtt gacaaattcc ttgaaaaata taattttatc147240aaaactaaca cattaagaaa tagaaaaatc tgaattgcct catatccatt taaaaattat147300
caaaaacttt cccacaaaga aaatttcaag tccaaatatt gtcatttatg aattctgtta147360aacatttaag aaagaaataa tgtcaacctt ttacaaagtc ttttggaaaa tacaggaggc147420aacacttccc aactaatttt atgagtttag ctttatccta taccaaaacc taactaagat147480actacaagga aaaattacag atgaatatca ctcagaaaca tagatgctct taaaaatatt147540atcaaaccaa attgaacatg taaacaatat atcataacca agtgggattt atcttagaaa147600tacttagtta aataattgaa aatcgatgta atttaccatg ttaactgaaa aaagaaggca147660aattgatgat ttcctccata tagctacagg aacagtggtt gatgaaacta gcagttactc147720aatgatgaaa acctctcagg aaagtaggat ataaggaaac tcctaaatct gataaaaaga147780tatttacaaa aaaatcttca ctaacaacat cccacttact ggtgaaatct gaatatgccc147840ctaagtttgg aaacaagaca cgtggtatct acactcacca cttatattca gcagtgcact147900ggaagtccta ggcctccccg ccctccttcg tgaaaaaaat cattgtcatc actaatgcat147960cccaggcaac tttgcatcca tgtagcagag tagtatgttt taccactcaa tttccagggg148020caagattatt gcctggacta gtgcacaact taattactct ctcactctga accttcccag148080tctgatatgg ttcttttctt ctttgctaca gaacaggcta gatttagcaa atgttggttt148140aacatatgga atgttactct gcttttatgc ttaactttgt aacacagatg tttgaaggac148200taggtaatgt gtacacaatt ttgtgaatta ggatatcatg tgttggcttt aatgcttttt148260tatgaaattt tgtaagtatg ctactaattt ttcttggtaa attatggaat gtgataatat148320aattttgtga ccttagtgaa atgtgcttgt aatagcacag atttataatt tgcatcattt148380attatttgag aaaaatagtc tcagtcctta ttgaaaatac cttagataca ttctaattgg148440ggcctttttg gcatctccct ttgaaacagt gctagagagt actgtgattg tgaggatatg148500aaaatataaa aatgctcatt tactagagga caggagcctg atttttcttg accagactta148560atttttcatc ttcattgaat agaaacttaa acaatttttt aaatatatag atgtagtatt148620attctataac aagacaattt aaatgacaga gtgacagaaa attttggtat aatttaaaag148680atggtttacc ttttatgtat tcagaaactt agattatact gatgctatat tttttggttg148740gggggccata tttttgtttt taaaaggata tccaagaagc aaacttcaag acatcaagta148800gccgactcct tgcagctgtt atgtcagctc tgtgtcacac gtctgttaag ctgacttcca148860tcttcccgat tgcgtatgat ggagaagtat tactacgatc aattgttaaa caagttagta148920cagagaacga ctcaacacta gttcatcgtt ttcccctttt ggtggcacat atggaaaaac148980
tcagccaggt aggtctgtct ctgaaatctt ttatacagag gcactaaaac tctaaatggt149040tttatttatt gcctctttta tacagaggca ataaaactat aaacacacca agctaagcgt149100atttagcttg gtgcttattc ttctctattt ttaagtatat ttacaaaatg attattttaa149160atatttttga aaaatgttca tatttaccta taaaatgaag tttttttgct ctaacggttt149220aatcacacac ctttaaaaaa atttgagagt tatacaatga atacctacat atttgttttt149280cctgaaccat ttgaaggtaa gttgcagata ctttacattc taatataata ttttagattt149340ctaagataga acaataagaa aataaaggat ttttaaaata tctacttaat aacactttgg149400tatattaaaa taagcactgt aaataatagg tataggaaac attcacataa atcattttca149460tgctggaaat cagctaaatt aatcatcttt tccctttttt ctgaaaaata aagtattttt149520cctttattga gactctgtga cttcttgaaa tttttctacc ggtaggggtt ggaattttct149580tatctccact taggaaaaca aatacagtag aaattataaa actacatagg aatcatatgt149640gtgtacaaaa agaattttgt taaaaaagtg atttacacag actacagttg cattacagct149700aaatatacta gaatttatta tctcagatta agaccttgtc ctatagaaaa agctttttat149760ttttgttttt taagactagc tcattctgtc acccagtcta cagtgcagtt gtgccatcgc149820agtttactgc aacctcaacc tcctggactc aaacgatctt cccacctcag cctcccaagt149880agctggggct gtaggcacat accaccacac ccagctaact ttttaaaaaa acattttgta149940gagacaaagt cttactatgt tgcccaagct ggtctcaaac tcctggcctc aagtgatcct150000cccatctcag cctcccaaag tgctggtatt gctgacacca cgcccagcca aaaaagcttt150060ttaaatggaa agaagttttc tgtctgtcat atagaattgt aaattgaaag ttaaataaga150120gaataaaata ggactcaaag ctaataaaaa catttcgtaa atgtatggta ctttgatacg150180cagagacaga taattcaaaa aagaacttct aagtgaaaag tggctccaca tataaccaga150240aagatttgta gaaattgacc ctgattcaag tctgaatttt aagagtatca gaaagacaag150300gttttcaaat tgagtactcc tatgaaggaa aataaaacaa taccttagaa ctttttctta150360tgtatcgcag agtgaagaga atatctcagg gatgacaagc ttccgtgaag ttctggagaa150420aatgctggtc attgttgtgc taccagtcag gaacagcctg aggagagaaa atgaactctt150480ctcctcccac ctcgtctcta acacctgtgg attactggcc agcattgtca gtgaactgac150540agcgtcagcc ctgggatctg aggtaataca ttatattttg acactggata aaaagcacat150600gtgtaagttt tttacacagt ttcttttttc aactattttg gctttgtgga agctaatttc150660
agattggatt agcaacccca atgcattgtt tttctttttt gcctttctct ccacctccta150720ctttctctga tttgttactt ctctctcacc agtttaccca gcatccacag gaatataaag150780tatacatttt cctcatagag caggtcgtca tctgccccat tgcacaagcc caccaccatg150840ccccgtgaaa agcaaagata gttctcactg ctggaagatg agtaacattg ctgaagtcag150900gccctactca tagtgcacac atagtgtggg aaaggtaatg aggtttagac ttcagctctg150960cccactcaac cgaccctaga gaaatacata cttaaaattg gaataataat ttttatttca151020cctctatcac aggttttttg aaattatagt aaaatataca taatataaaa tttgccattc151080taatcatttt tacgtgtaca gttcagtggc attaaatgca ttcactttgt tgtacttagt151140tgtacattat cacaaagttt tcaaagtctc aaatattatg tttgagaaaa tcactttgaa151200agtgccttta cattttgtaa agtaacatta ttccatttca ctgccttact ctttcttata151260acttaactga atgttttgac tgaatttggg gaatgtttct gttttttcct cctaaaaaac151320agacagtaga ttggtttgga aaatcatgtc ctaatatgaa atatcttaca tcattcaaat151380gaatgtttaa taatcagcat atattcagtg gccctagctc ccaagttctt gtcaaggaac151440ttcttttctc tattttactt ttataattcc taatctttgc tttttttaaa aaatttattt151500taacacctat tctgaattaa cccatccaat atatacacat tattgattaa ttgtgacagt151560ttagccttta gaatttcatt gtatgaaatt catacgtaag aaattcgcat acatatggtt151620catcgcatac acttgagcta attttagtct ctcatcctgg gaagcacttt ctaagtttct151680tggttaactt tgagtttggc ctcagagacg attgaagccc ttgtcagaaa ttttttaact151740ttttacattt actcatttag tctataatac cttcctttga tagtatactc tgcaggatgc151800aggaaagtct ataatgtgta gatccccttc caaagtttac taaagagttc tcccctttaa151860acttaccttt ccaatgaaaa ggaataccaa aagttacagg gtgcctggta tacaactttg151920ggagttccca caggtcctgg cttagagatg aatatttctt tctcagagaa gtttgcaatt151980tttgttagaa tcataaactt ccatttttgt atctatttag tctcccaaaa ttctgtagcc152040aagttagatt agaagtaggc tttgtaaata aaaagagcga tttcaaccca aagtaagttc152100tctaattctc tctagtgaaa gtctataatt agtatagttg cacatgtgta tgtgtacgta152160ttcaaatact gaatgctgtt acaaaaatat cactgtatat attcgtagta ttcaatctta152220gggtatagac cattttcttc aaatgaagtt ttacttgcaa attcacaggt aagaggagag152280aggacatagt ctggttgaag aggggaacca taaatttacc tcttccttgc acacctatct152340
ctgcagaatt tctagagctc tgtgttgcat agtttgaaaa ctgttgatct atagaatttc152400gatgaaattt ttagcatctc ttttcaaagt ctgatgattt attcatacat tatgactgat152460aagacaagct tgtacttctg acattataaa ttaagtggaa agtatagttt catatggcta152520aaggaagaaa atactgtcaa gtgacattga tttgtgaatt tagaatgata aaatctggtt152580ttattgtgag ttaatctctc agtagaacat acctttagga atgtttatat ccacttatta152640aataaaggtt attttattag acttcttaga aacctttgga tggttttgtt aaaggtatac152700caactactga tacaattgct tttattgttc acaggttgat ggacttaatt ctcttcactc152760tgtaaaagct agtgctaacc gatttacaaa aacaagtcag ggcagaagtt ggaacactgg152820gaacgggtcc cctgatgcaa tctgtttttc agtagacaaa cctggaatag ttgtggttgg152880tttctctgtc tatggaggag gtggaattca tgaatatgaa ttagaggtgt tggttgatga152940tgtaagtatc acccttttag tatttatcct gattagtggg ttgtgtatca ggatttacca153000ttgtcatagt atgttcaggg gtttactaga tcaatcactg atgaatgaca ctaaagaaat153060tgaaaaagac tggccacagt ggctcacacc tgtaatctca gctctttgag aggctgaggc153120agttggatga cttgagcaca ggagttcaag gccagcctag gcaatatggc aaaaccctat153180ctctacgaaa aatacaacgc acgcctgtaa tcccagctac tctggaggct ggggtgggag153240gatcacttga gcccgggagg ttgaggctgc agtgagccat aatcatgcca ctgcactcaa153300gcctgggcag cagagtgaga cccgaccctg tctcaaaaaa aaaaaaaaaa aaaagaaaga153360aaagaaattg aaaaataatg attttacctg attgtttgtg ttccatcttt ccaccttggg153420tcactactgc ttcatatttt ttgtactttc agaaagagaa agatttaaag tagaattaaa153480gactaaaagt gtttagctaa gtccctgatg acaagtcttg agtagttttt tttttttttt153540atattgtcat ttttcaagaa acacaatgtt gagatggact atgtcataga gtactaatgg153600gtgattctta gtgaagacac tatttgttga ctgattgagc tatatctttc tatattctct153660aaaattaaag tacctgagtt ttattaaaat gggacagatg gggtagcatg taaatcattg153720cctattaaat agcaagtcta tctctatctc taacttgatt cctttcacta gttaatttga153780atttattccc tacttcatta ggctttataa atgtgaagaa gtagaaatag tttaagtcat153840cagatgagaa aagcttattt atgttgtagt cttctcaaga aatcacataa aaatgtatat153900agtaccccca actttttctt tcatatgtat gggaaaatac taatggctaa ttttatattc153960aagaaaataa attttaaaaa taataattat aaatcaaaat tgttcataca ataaaattaa154020
gagtctgttg gaatttttgt gtttcatttt ttttttttag agtgaacatg caggagattc154080aactcattcc cacagatgga catctctgga attagtgaaa ggaacgtaca caacggatga154140ctcacccagt gatatagctg agatcagact tgacaaagtg gttcctttaa aggtagtttc154200aactgaatgt ttctattgtg aataagaggt attattatgt agttaaaata gtgaagagta154260ttccatgaat tttgtttgtt acctatagct atttgtaagt tatcctttat atggtactta154320aatatttatg gagtacctac tacgtaccta atgttgtacc acattatatt atatttttgc154380ataatgtttc ttaaagtcag gagtagtagg aagttttgta gtcaaacaga cttagctttg154440gatacctgct gttccacatg ctgactttaa gacactggca gatcattatt taaccacttt154500gagacttatc tgtagattgg gaagaataat acctgcattg tagtagtatt gagattaaat154560aatggaaata aaacacctgc cttagtgtct agcacaatat gaaaaataaa tattagctcc154620tgtcttttct tctcttattg tgccctaact catttaaaaa aaaaaaaaag tgtttttgtt154680ttgttttgtt ttgtttcttt attttttttt aattattatt attatttttt tttagatgga154740gtctcactct gtcgcccagg ctggagtgca gtggtgtgat cttggctcac tgcaacctct154800gcctcccggg tttaaatgat tctcctgtgt cagcctcccg agtagctgga attacagcca154860cgtgccacta tgcccagcta attttgtatt tttagtagag gggtttcact gtgttgacca154920ggctggtctc gaactcctgc cctcaagtga tccactccgc ttggcctccc aaagttctgg154980gattacaagc gtgagccacc gcgcccagcc tatttcctaa caatatttgg caaaatagag155040aaattttacc acatcaaagt aattatagat gttaaaataa aattaaagta tttctgaatt155100caactgaaat aaatttcatt atagagcaga attttggata agatcggtac acgagaggtt155160tcatcaggaa gaatttagat actgaaaaac caaaaagctt ttattgtgaa atttatatca155220tgggtcataa agcattagtt tctatagctc acattttaaa agtacagtgc attatatttt155280ggacagcttt tttcctatgg gttttttttc tcatgtctgt ttgcctatat atttctatct155340ctgagttttt cttaaaaata taatagactc accatttttt tcctcacatc taaaattatt155400ttaaaagtta aactagtcaa ggaaaagtat cagcttatgc aattaaaatc ccttattgcc155460gggcacagta gcgcacacct gtaattccag tacttttgag acaccaggga gggaggatca155520cttgagcttg ggagtttgag gctaggctgg gcaatatagt cagaccttat ctctacagaa155580acaccctaaa aaattaactg agtgtgatgg aacacaccgg tgggaggatt gcttgagccc155640aggaagagga ggccacattg aaccaaaatt gcaccactgc actccaggct gggtgacaga155700
gcaagatcct gtctcagaaa aaaatatata tatataatat ataaaatata tatatattat155760atatatataa tatatattat atatatatag tatggctaat attctagcag taaaattaag155820agcaggcact agttacattg gttgtgttca gacctagttc catcacttac tagctgcgtg155880actttgaaca agttacttaa cttctctgaa cctcagattc ctcatctgta aaatagggat155940agtaatgata cttaccttat gaggtcattt taaggattaa ttaaattcca taaagtagtt156000aggacagttg cttttatgca acaggtgttc agtaaatgtt agccgtcatc gtgtttgatt156060cattaaaaac ttatttaatt atatcatctg taaatatgac aaagaatttc agtatatttg156120cctattttac tgctagagtt ctttttatat atcctctgta cattgtgaat aaaagatgtt156180ttacatcttt ataatgaagg aaaatgttaa atatgctgtg cgcttgagga actatggaag156240ccgtacagcc aatggagatg gaggaatgac cacagttcag tgccctgatg gtgtgacatt156300cacattcagc acgtgcagct tgagcagtaa cggcacaaac caaaccagag gacagatccc156360acagatactc tactataggt gggtgaatgt atagagataa cggaaatact ttacagtggt156420agaacataca cagtctacta ccaggtaaaa ttgctaactg gatacttata attaaattgt156480acagtgtatc ttcttagcac atacttgtac aaattcatat aaagaaatct ctttttaata156540tataaattca atgtttgcta caaaagttaa tgctttaaaa tatttttgct gttatttctt156600caatatcatt gaaatgtttg tgtactgggc atatgtgctg ttgctgtcac tctataattt156660ctggtctgca atttcaaatt taaaggatat tcatttcttt ttataaaata cctcaaacta156720atgatcagat agactgtact cagatagtgg tgttgccttg atcttgttat ctagtcacag156780aatattttga tgtctgtgaa tcttggacct gccccctggg gccctagaat atataatagt156840gtgtttctta taatgttttt aacaaaaacc attccttatg taaaccttgg aaaataaaag156900aatgataaca acccttgggt taaaaaaatc ttttaaataa acatttctca ctgagtctga156960atttttcttg tagcggcaaa gctggcgtct taggtagtca tgagtaccat ttaccacatt157020tgtcctcagc ctgacttggc attaaatcac tgaatttcca tgtcactttc agaggcttgc157080tgcttcctct ctttccttca tgaagagtct agggatggtg cctacgtaga tagtataaaa157140taatttccct agatatagat tttctgcttc ctctttccat cagaaataga atagaaatag157200caggcagcaa atagctatgt agttcattgt tgctgctgaa atacaaaagc tgtctagtct157260tttgccccag tcaagaaaag ctttctgtta cataagcact acaataaata gtacattatt157320ctctttctta aactatggat taaaaactct gttatgcgta agagttacgt gtttcagaaa157380
gctggagagc cactaagagc atctgccatc caaactttat atcttataca aaatacagtg157440ttttgagtat tttcatgttt tctattaagc tttaaatacc cttagcttta cattttttat157500aaatgaaaaa tgttaaagtc ctttagtttt aaataccatc ctgctctgat agctcccaag15756atttacatccc tagccttcat gacctctgag ccctggactc cgggtctctc ttctgacatc157620ttcatttgga tgacactagt ggagatggtg ttagttactg tgtgccagga catgctttga157680gtatgttacc tgtgttattt caccttgtcc tcacagtaac cccaggaggt aggtactttc157740cttatccctg tttcacagat gatggaactg agacgctgaa ttgtctagag tcacacctct157800gttaagttac gctactgcga cttgtaggca ggaagtcaga tttcaggtga tacaccctta157860accagaatgc tgcactttct ctttaaggat gttcgttata gttggctagt agacatctga157920ggtttcatgt ggctagaata ttcatctccc ttccacagtt acagccagac accttggagt157980cattctggac ttctctttcc ctcatacttt acacctagtt catcaagaag tcctgtcagc158040tgtacttcta aaatgtccta aatgtgacct tttcttctac ttccatattt aatagtctag158100accaagccac tgcaacctct acctgctaac tgtaataagc ctctaaacta gtctccctcc158160tttcactatt ggtctcctac aatcagttct ctacagcaca gccagagtga tctgataaaa158220atataaatca taccaagttc cacccttgct ccaaacccag cttcccattc tcataccagt158280ataaaatgta aagtcattat gtggcctaca aaccttacac aatttgactc ttgtttccct158340tcccacccat cacgcttcac tctttctcct tttcttccag ccatacatgt cttcattctg158400ttcctttaat tcaccagtat gttccttttg ccctgacagt tttccctcag ctcttcgcgt158460gggtctttaa aaaaaaaaaa aaattcaggt cagctgggtg cgcggctcac tcctataatc158520ccagcacttt gggagtccaa gatgggtgga tcacgaggtc aggagtttga gaccagcctg158580gccaacgtgg tgaaacccca tctctactaa aaacagaaaa attagctggg cgtggtggtg158640catgcctata atcccagcta tttgggaggc cgagggagga gaatcgcttg aacctgggag158700gcggaggttg cagtgagccg agatcatgcc actgcactcc agcctgggcg acagagcaag158760actctgtctc aaataagtaa ataatttaat taattaattc aggtctttgt taaatatgag158820ttcctcggag aactgatctt atttaaaata acacccttca ctttatgccc tatgctgctt158880tgtttttctt caaagccctt atcattccct attattgtat agttgcatgt ttactttttt158940aacttttaaa aagctataaa ttaaatgagg atctcagatc tagatatgat ttctggcact159000ggatcatgtt ttacttacca ttgtaaaata atctcatgtt tgctcatgct acaggagtga159060
atttgatgga gatttacaat cccaacttct gagtaaagcc aatgaagaag ataaaaactg159120tagcagagca ttgtctgttg taagcactgt cgttcgagcc tctaaggacc tcctgcacag159180agctcttgct gtggatggta atattttttc tttctcagta gtcatatttt agattcttta159240ttcactaaat ttttgcatag aattattgaa gaggagcctt ctattataca attttataga159300atctaagtgt tatttataga tccacccatg gattaatttt ggataggtca cactctccat159360actgtaatgc agaattattt agaatgctca aaaggagtta ccatatattc ttattttctt159420agttaattta gtaaaagaaa aattgtattt gagttaaact gggtctttat tcttctttta159480gtaatcaaaa agctttttct tcctctctgt tctttttcca gtaatgtatc gctatccacc159540caaaactctg gggtacccta accatctcct cagaggcact tgtctaaatt cagctctatg159600agtttttcca ttggaatctc tctagtttgt attctcctct ctttcacttc ttctcctgct159660gtcatttaga ctgccatggt gcaagtacat ttcatttttt aatatagggt taatatcccg159720ataatctcac tgctgccttt cccccattct ccatctagtt gtttagttat tcttcacact159780ggcagcagag ttatcattat gaaaacatgg ttatggccgg acatggtggc tcatgcctgt159840aatcccagca ctttgggagg ccgaggaagg cagatcatga ggtcaggagt ttgagacgag159900cctgagcaac atggtgaaag cccatctcta ctaaaaataa aaaaattagc caggcgtggt159960ggcaggtgcc tgtaatccta gctactcaga aggctgaggc aggagaatcg cttgaaccca160020ggaggcggag gctgcagtga gccgagatcg caccactgca ctccagcctg gatgtcagag160080tgacactcca tctcaaaaaa aaaaaaaaaa agttacctcc acctccttat tgtataacat160140taattcctaa acctagttta acatcagaac cacctggaaa gcttgctgaa aataaagact160200attagattta tccccaggtc ctgaagctag gctaaaaaac tattttttaa ataagtgtgc160260agcctgtttt gatataacag cagacctagg aatcagtatt tgggagaata attggcttat160320aaaataaagg tcttcacagt ataggctcag cttatctttc cagcaccatg ttcatggata160380actgactaaa atgttaatca gcaaagtgct tttcttggaa aaacagaaag caatgtagct160440taataacagt aaacattttt ggagtgctga atatgtgtag gtgttgttct aagtgtttta160500catatattac ctcgtttaat cttcccaaca tccaaatgag aggaatactg ttgtgaacct160560aagttacaga gtaagtaatt tgcctaatgt cactgataca gccatgtttg gtgccagaat160620ggtaatttaa aataaagact ccattaccct aggttctgat tccagctcta ttattttaca160680ggtacatgta cttatgtatt aagtattttg ggtaagatac ttaaacccta tgtgccatgg160740
ttttcctttc tgaaaattgg ggataataaa agtatcttat tgtgagtatt agttgagata160800aggcatgtat tctcaaaagc ctttagaata gtaccaggcc cttgacaagc acccaattgg160860tgtcaactat agtttcgctc aaaagagcag aatatagatt tagtacagca tgtatgcagt160920acaatgaaat taccttctga ctatagtttt aatgcctttt atgccttttt ttagctgatg160980acattccaga actgctgagt tcttccagtc tgttttccat gctgctcccc cttattatag161040cctacatagg accagtagct gctgctattc ccaaggtgtg taatttaatt ctataacttt161100gaatgttttt tttaaatatc ttttttaaaa aaagactttg tgtcttgtct ttacaaaaac161160ttgcatcttt gaggcatctg ggatataggt aacaagcaga gatagatctg ctaattatta161220aacttctctc ttcttagtat tgtttactgg attgctgtgc ttatttgttg gctttttttt161280tttttttttt tttgagacgg agtcttgttc tgtcacccag gcgggagtgc tgtggcgcga161340tctccgctca ctgcaagctc cgccttccgg gttcacgcca ttctcctgcc tcagcctccc161400gagttgctgg gactacaggc gcccgccact gcgcccggct aattttttgt atttttagta161460gagacggggt ttcaccgtgg tctcgatctc ctgacctcgt gatccgcccg cctcgccctc161520ccaaagtgct gggattacag gcgtgagcca ccgcgcccgg cctgttggct ttacatttga161580agaatctgtg aatgggaaat agattttagc aattttagga atatgatatg taaggaaaga161640ttatgttaac tgaggttttt cacatttatg aggttgatgc caaatggtct tcattaataa161700caatagcaat gttaaaggat aagagtgttt taaaatagtg acatattcaa gagatggaag161760aaatgataaa ctattgattc tttggcataa ttaagtaaaa cctgatttac ttcagatcat161820gaaaaaacaa gtttttaaaa atatagattt agatattgtc atgtaacatc taagataaat161880ctttttttaa tcaattgaaa gatttctctt tggacaaaaa cttaactagt ggaaaattta161940gatggaaatt gttattagag taaccaacaa ttattgtaat gcataatttt agaaccactt162000tcacgtgtat ctcatttaac catattttta tatgtgtact aggtggctgt agaagtcttt162060ggccttgtcc aacaattgct tccgtcagtt gccattttga atcagaagta tgcaccgcct162120gccttcaacc ctaatcagtc gacagatagc accacaggaa accagcctga acagggcctc162180tctgcttgta caacctccag tcactatgct gtcatagaga gtgagcaccc gtataaacct162240gcctgtgtga tgcattacaa ggtaggcacc ggttcttagt gtgattcgaa tgtaaaactt162300ggcatgaggt tctctgatga tatcttaaag aatgcctggc ctgtatttac aagtgtattt162360tcttttatag tatttacata tagagaatca gcaggtgatt aggcactgtt ggatatataa162420
aataatttaa gatggaattt ttatctcaaa gatgataaaa ttttattggt ttatgagagt162480tgttcttaaa tcttcctaca agtatagtgt ggtatataat gctaaacttc atgctgtgat162540ttgcaatagt agtagatata agcattaaca attttaaatt gtattttttg aactaacatt162600atttgtacca cagcctgagc tctgaaaatt tacaacccca atgtaagatt taaagtagtc162660tatacataat gttaaaaaaa ctatgtataa aatgtttata aatttaatgt tattagagag162720tattcaaaat ccgtattctt tgttttttca ttgtatcatg caaattaagg aagagaagga162780taaacttgta ttttgtgtgc ttgtaaaata ttccactttt catggtagaa actattaagc162840atatagaagt aaacaagcta gtacagtgca cttgtttatc catcatccca gattcagcag162900tttcaactgt tagtcagacc tgtcttattt attcccaacc tacttcctaa cctcctatat162960tgttttaaag cacatttatg atatttattg atacaatgtc tccaaaatat aaagaccctt163020aaatataacc atagtattat cacattgaag aaaacaattt aatgtctgca aacatcatgt163080aaatttttaa taatttgagt tagaatccat ataaaatcca tatattgtga ttagtatgtc163140tcttaagtcc cctttaatct taagtccctt taaatctata agtctccctc tgtctctctc163200tctttttgtt cccttgagtt ctatttgttg aagaaactgg atcctatgta tttgttagat163260gggagcctct tcagcttacc tgctggttct ttcagacacc cctcttgcag tcattcctag163320cttccttgct ttctggtagg ataagatgtt ccaggctccc tttatatgtt tgatgcctca163380gacttggaac taaccatttt ttcaaagtgt cctgatttct gttatacata cacacacatg163440ttatatttat atatgtatat acataaatgt atatatgttt atgtacagat agatttatgt163500gtatagtttt ttaaagataa aatacctcat gagtttatac tgttgatact aattcaaaca163560aggaccacag acacacctca gtctcctttc tcttatatct aaaattccta ttagtgacac163620tgggaatatc agaattagaa aatcattaaa ttatttcatt tggtgtgtgt gtgtgtgtgt163680gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtatgtgtgt gttttgtctt tgaagtatat163740cccatctaag tgtatgtcaa attactgtgt ttaaagtaag ttggaatgct ccctctgcat163800gattatgcat tcattggata tacacttaga gagtcattta ttttatctta gattttagga163860attgcttttt aaatttggat tttgttttat aattctttac ataattacat actttgaagt163920caaatttatg aaacaaatct agcttttatc tctgtcctct ttactctgtt cctccttctc163980tgtcataggt aacattttat tctattttaa ttttgtactt tatcctgttg tgtcatttaa164040taaaagcata gacacacaca cacatacaga tgcagatgtg tatgtttgtg tatatatata164100
tctgtattca tgtcactttg cctgttgacc tcaggtgaca ttcccagaat gtgtgaggtg164160gatgacaatc gaatttgacc ctcagtgtgg tactgcacag tcagaagatg tccttcgttt164220gttgattcct gtcagaactg ttcagaattc aggatatgga ccaaaattga catctgttca164280tgaaaatctt aattcatgga tagaattaaa gaaattttca ggatcctctg ggtggcctac164340tatggttttg gtgttgccag gtaagttttt tttttcttct gttttatgtg gtaaaataag164400tgcatttatg tatatttagt aagggtgtat cattgaaaat gtttatctaa tttttagaga164460aatttattaa agacctacta tgtatctgga ttgcattcga ggaataaata aggtaccctg164520ggcaaggggc acagcctgat ctgagttttg gagaagacaa tgttttaagg attttgtgaa164580aaaaagatta gcttgagcaa agcagagtgt taacgttgag gagtagctag ctgtctgtgt164640agttgggtcc aaattataga aaacaatgga ttagatcttt tattttccca ggaagtgcta164700aattttcaga acttacctaa ggactgaagt ccttcattgt aagagaattc gtaaaccact164760ttcttgtgtg aagagaaact atgttttaaa acttatggtt tgttgcattc ttaaccagtt164820aaccaggact actcttgtga tccttcaaac taataggcaa tttcatttca gaaacttctt164880ctttccactc tgacctgcct atttgtttgt ttgtttggga aaagatcaga aaaagccaca164940ctccagggta gaggattgcc cactttgaaa aataaaaagg ctaacctcag tatttgacaa165000ataattgaaa aatgattgcc catgtaactg aagtacttta ttaagcaggg gagtttgatt165060atgaaaaaaa gtaacaattc atttgcgata gacctgttgt ttctagtgtt atttttggta165120cagtttcctc aagtagatat aggaacgctc cccgccgcaa gtctatttaa gttatgtagt165180atatgatttg tgtttttacc tatcttgtga taaaagagaa agtccattgg ataaaagaca165240gggaaatttt tataaaattg ttttagagtt tgaactcaat tctggcattt ttattaggaa165300atgaggccct tttttcattg gagactgcat cagattatgt gaaagatgac aaagcttctt165360tctatggttt taagtgtttt gcaattggat atgaatttag ccctggacct gatgaggtaa165420gaatgaatta tcttttcatt cttttaagga agattgtgtt tctaagggct agtgagttgt165480gcttctaact gacttaaatt gtactgttgc ctctattcga aaaattgggg cctggtgcag165540tggctcacac ctgtaattgc agcactttga gaggtgagag aatcccttga gacagtttga165600gaccagccta ggcaatatat cgaaatcctt tctctacaaa aaaaataaaa agaaattaat165660tttaacctag gggtgtgcaa ttctaaattt ttgtagttta tgtgcagtgg tctgttttgc165720tacttttaat acatgaagag gatggagttt gtgcaacatg acccagcctt tgaagtatag165780
ttatacctgc cctgaacctg ctcagttggc tatacctttc tgagtaaaca gtagaacctg165840ttttcaaaaa ggggctatca tcttgtctaa atgttttaaa tcatacaata agataaaacc165900aaggacaaag tgggccaaag tcatgaatga acagttattt gaacaatgaa cctttaaacc165960tgaaccttat aaccagcctg agccacaaca ttgagtactg aatctcatct actctcaatt166020taaccattag cttcacgtgc ttccttctca tttcaaaagt aatgcttgaa ttcaaatcac166080attttgttga taatccgtat tatcattcaa acatttttaa agcagcaaat tttatgcaat166140tccataggga gtcatccaat tggaaaaaga attagccaat cttggtgggg tttgtgcagc166200agctctgatg aagaaggacc tagcacttcc tattggtaag tgtggccaat actggatgtt166260ttttgatagg gttttgtttt gttttgtatg tattataaaa gggccaaaaa tagatttgca166320aaatgaattt tgtaatctca tatattcatg agcaataact gcttcatgta ctggtaattt166380gagtttgttt agagactgaa gggtatcctc tgaccttttt taatttacac acgcacatgg166440acaccctttt tatgcatata catgctttga gccaggcagt gggtatagtg tgtatattca166500gccaagcact gaatatatag tattaaacca aaagttctag atatttctta aatattctag166560tggaaatatc aagtagacaa ctgaatactc attgtctaga tcagagaaca ggtcgataga166620aatgtaattg aatatgatcg ttatttaaag acatgggact gtatgaaaga tattctacag166680caagtagata agaaagagaa gatagcttag gattaaagct gtgacttatc aaaatttcaa166740agatgcacag aacaggaaga ggaggctgca gatgagactg tgaaggaaca gtcagtgaag166800aggagagaaa ccaggagagg ggatgctcct gggatttttg catgctctga gaagtgaatt166860aaaatctatc attggacttg aagacttgtt ggttgcttgt atatgcaata agaaatatgt166920tagcaaaatg agagaactgt aagtactttc agagtagaga gggagaatgg aaagtaagga166980agggcggtcc atttgaatat aggtagctct ttagaagaat ttttcattga agcagaacag167040agatgaggca gtggcagaaa tggtactttt ttatatggag caaatttgta tgatatatag167100attaatctat aatcaaggga gtaaagttga aagtaaaaat gcttactgat tctctaatat167160ttatacatgg tttaaagttt tattttctta cccccccaaa atccatcttt actccttcat167220tgaagattac tcttcccact gttatttttt tcctcccttt tcttcctctg cctttccttc167280tttattccct tttctcttcc tggtcccttt tattcttctc catccctttt ctcccactta167340atattggtat atatgtatgg aaatgagacc ttgtattcta gccacatgag gaaaattgct167400agcaacctat ttttgtcaat agctgttaaa ttttacatta tatatacttt ttgacataga167460
aaatttttat acatacttgc acaaatactc aaaaagtgta tgttgaaggg tggattaata167520tagcagtgct tgtgattgta aaacctagaa acaaccaaaa tgtctttcag ttgggaactg167580gttaaataag taatggcata gccattcaat ggtagtatat gcagctcttt gaaaaagtag167640acctatacag cctaacaagg aaagctgtcc aaattatatt gagaaaagca agttgcagaa167700gaaggtccca tttttgcata cattactctg tatattacaa aaaagtatga tcatgcctgt167760aatcccagca ctttgtgagg ccaaggtggg tggatcacaa ggtcaggaaa ttgagaccat167820cctggctaac acagtgaaac cccgtctcta ctaaaaatac aaaaattagc cgggcatggt167880ggcacatgcc tgtagtccca gctactcggg aggctggggc aggagaatca cttaaaccca167940tgaggtggag gttgcagtga gccgagatcg caccactgca ctccagcctg ggcaacagag168000tgagactcca tctcaaaaga aaaaaaaaga gtatgtttat gtgtggttga aacacatata168060cctttttggt ttgtttgcac accaagtttc tagaacaaca cataggctgt tgacactgga168120tagttttaag ggagcacggc actggttaga ggtttagaat tttttgttct acactcctat168180acaatttgag tatttcccat tgaacaagta ttactttatt agtaacattt aataattaaa168240ctatcaaaga aagatcaata aataaaatat gttctatatt aactaataaa ggagaaaatg168300gctaggggat gcagtcactg ccttatattt ttctgtgagt ccctgttgtt tactaaagtc168360atagctcttc tgtaaggcaa ccaaaatata aaggcccaga tctgaaataa tttgttccct168420ctttttttag acaaaacgaa tacactaagt tcagaaagta tacatttata ttcataaatg168480taggatatat ctttggcaaa tcacccatgg atgcttcttg agtattgcct tgagaattgt168540agtatattcc cactggaagt atcttatata ttaaactgta tgccactagg attttttcca168600gaatcacaaa tcttttcaat ctggaatagc agaattattt ttttaaaaaa agtgtaatga168660atgcaaagga ctagaaaatt aaactaaact aaatattgac atattttagt aaaactcttt168720atttacacaa caggtaatga attagaagaa gaccttgaaa ttcttgagga ggctgcattg168780caggtattgt cccaaatgtt ttaaggataa actttgtaca ttaatttaaa aagtattgtc168840actcagatca gcctatttgt cttgaccatt gggttcattc attaagtact tcactttatt168900cattggcatt atagagataa ataacttatc attgctccct ttggtgggag aaacccatgt168960gagccctatg tatttggtgg gtaattgact ctggagttag acaaaaatcc ttcgggagaa169020aaaaacaaat caagtgccaa gggtgcggag gatggcttgc agaactgtgg ccatttaaat169080tgcatcttga aagaatttgt ttttcttttt ttgcctgcta gagaaagggg aaagacattt169140
cacaaaggat gtatagttgt gttataagaa gcagcaaaag gtgaggttgg tacagttggt169200gagctcagtc tgtgacaaac cagtatgtca tattaagtgg ttagggctag ttgcctgtag169260acaattggga gccatcattt gttgttttta ttattgtttg ctttaccttt tttgctcgtt169320taggcattaa ctagcacatt tggcactgaa tggaaataca tgagtactat atttcagtgc169380aataccccct ttggatctgt tacgtcaacc taatatttct caaaattgcc tagagatgac169440tacattgttt tcttacttca ttagtctgac ttcagctagc tagtaatctg taataggatg169500gaaatatcag aatctgtaga cagcatttgt caaattgtaa tacctactta ggccaaacca169560gagtttctga tgtactgcta agtgagcccc attggatacc acacactgag aacatcagtg169620atggggaaaa ttttgcatct agcaatggtg tgaagataga agaaaagttg agagtctctc169680aactggatca gtgttactca gaccctagga tgtgatagat ttgtaaaatt ttccaaaaat169740atttttcctg agaacttatt aagtaaaaac actctttaaa aatgtactac tttttatcag169800caatgtctta taaaataaaa gacattttaa tagccccaga tggaaaggtc ttagaatgga169860agactctgtt atagccttag tttttctaat tctgccatgg atcagagaac tttgggccca169920catttggaaa acaccaattt agatcatact tattttctat actaggtata tgtagttagt169980aatagtgatt taaataatat ctgtagagca cttcataggt ttcagcatct ttaatattat170040ctctctgttt ttataattac acttcgaaat cagtattagg tcttgcatct tacaattata170100ataactgaag ttcaaagaag ttttgtgact tcttgaagac tcagaaggga aacaaaggct170160ggtacccaga ccttgtaatt gtgaaggcta gtgctttaca ttttacttat ttcctgaggt170220gaattaatca aagcggtaat aatgaaaaca tttatttcat ctttgtcaag cactatttta170280aacattttgt gcatttttaa ctccccacag caacccaatg aattaaaaag ttttatctcc170340atttacaaat gaggcataga gcatttaggc agttcgccca cgatcataca gctagtgagc170400aactgcactg ggatttgttc gctggcagtt tggtgacaaa agaaaccccg tctcattagc170460tgggcgtggt ggcgcatgcc tgtaatccca gctactcagg aggctgaggc aggagaatcg170520cttgaacccg ggagtcggag gttgaggtga gccaagatca tgccattgta ctccagccca170580ggcaacaaga gtgaaacgcc gtctcaaaaa aaaaaaaaaa aaagtttgtg attttgaaca170640ctgtcttctg atcataggga gctgtagtgt tgtcaataat gttaagaaac gtctgagttt170700taaaaagcca ttggcatctt atgtgtattc cttttttaaa gttaaatctt tgtaccttaa170760gattcaaaat aaaaataaat tcaacagata tataataaac aatatgtgtc aggcaacatt170820
ctaggtgaag gagaactatc aaagagtttc tattagtcca ttttcacact gctataaaga170880actacctgag gctgggtaat ttataaagaa aagaggttta attgactcac agttccctac170940atggctgagg aggcctcagg aaacttacaa tcatggcaga aggcaaaagg gaagcaaggc171000aagtcttaca tggcagcagg agagagagaa ggggaaaggc ctacacactt atcaaacaac171060cagatattgt aaggaaaaca gcaaggggga agtctgcccc catgattcag tcacctcccg171120ccaggtcact cccccaacat gtggagattt ataattcgag ttgagatttg ggtggggaca171180cagagccaaa ccatatcaga gttcatatat ggtttataat ttcagggatt attatggtat171240ataaggtgtc atctagacca gatatggaga gatctttatt atgttattgt tcaagaaaaa171300aaaaaatggt gtcccaaatt gcctcttttt ggcatgtaag gtagttattc tgataatcta171360acatgtatta aacacttact atttgttagg caatgtatta tatactttat atgcattatt171420tagtttgaat tttttaactg tgtaagagag attatttgat gaccagttta tacattttac171480agattcaggg atggtaactt tttagcttaa gagcacacag caaataattt gtccaaagcc171540aggatttgaa tttaagtcta tttgattcca aagatgatta tttttgaaac tgtataattt171600caaatttgat agtactagaa atgtaattat tttctatgaa aaaatcttac tggaatttat171660tttatagtca gtaaaaagtt ttcagagttc cagcatttaa aacaagtctc tccaaaaaca171720taagacttgc ttgtcagata ggtgaaacct aaaatttgtt ttatctgcct cttcttctta171780actttagtaa ggcttaggca ggctaatgag atgtgtgtgc tgccacaatc aggcttccac171840ccacttttta cccaagtcaa gaccttggtg cccacccacc caagagaaca gacacacctt171900actcatgata gctgcctgta agggggactg aaaaagaggg aagccggatg cagtggctca171960ggcttataat cccagcattt tgatagggca aggtgggagg attacttgag gccaggagtt172020caagaccagc ctgggcaaca tagcaagacc ccatctctac aaaaagtttt aaaaaaaatt172080agccagccat ggtggcatac gcctgtaagt cccagctact tgggaggctg aggcagaagg172140atcgcttgag cccaggaggt tgaggctaca atgagctatg atggcaccac tgtactcctg172200ggtggcagag taaggccctg tctcaaaaag aaaaaagggg gagacatcca cagttgctga172260cagaagggag ctctggccct gaaaaatgag gacataacat atagaggact ggcatggaag172320gagatacacc attttatcca gcatgcagat ctctgacaag gcatttattt ttgagggagg172380aaagagactg gaaatattgt ctcatatttt tctctcctag gaatttccta ttctgtgggt172440taaaagaagg aacagtgggg tacgtggaga aaatgatttc tccgtaaagg cctatctgaa172500
agtatctgca tgttgaaaaa ttttatgtaa ttaaagtttt atttttaaga atatataaat172560attctttttt acttaacagg tgtgcaaaac ccattctgga attcttggaa agggtctagc172620tctttctcat tcaccaacta tattagaagc acttgaggga aatttaccac tccaaatcca172680aagcaatgaa cagtcttttc tggatgattt tattgcctgt gtcccaggat caagtggtgg172740aaggcttgca aggtaaatgt actttaagtt acttacttta ttttagggct ttcatctttc172800ttcatttctg aaagagggga gtcattagaa tattaaaata ctactttaaa aaagtctggt172860ttatgtatgt ttaacttgct gaaatataaa atgatagtta atatttttgt ttggcggttg172920tttgtttgtt tgtttttgag agagagggtc tcactgtgtt gcccaggctg gagtgcagtg172980gcatgatctc agctcactgc aacctccact tcctggactc aagcgattct tgtgcctcag173040cctcccaggt agctgggatt acagatatgc caccacaccc ggctattttt cttttttgta173100ttttttgtag aaacagagtt tccccatgtt gcccaggctg gaatagttat gatagtatat173160gataagacca ggtgcaatgg ctcatgcctg taatcccagc actttaggag gctgaggtaa173220gtggattgct agagcatagg agttgaagac aagtctggcc aacatggtga aatcctgtgt173280ctacaaaaaa tacaaaaatt agccaggcat ggtggcacac tcctgtagtc ccagctactt173340ggaggctgag gtggaaggat tgctcaaacc ctggaggcag aggttgcttt gattgtgcca173400gtgcaatcca tggtgaaacc ctgtctctgc aaaaaataca aaaattagcc aagcatggtg173460gcacacacct gtagtcccag cttcttggag gctgaggtgg gaggattgct caaaccctag173520aggcggaggt tgctgtgatt gtgccactgc aaccagcttg ggcaacagag caagacccta173580tctcaaaaaa aaattaacta attaaattaa atttattata gttgttaatt gattattttt173640gttgcttttt gtttttagga tgaaaaggga aaaaaatatg ctggcctgca tgattttact173700agtaaaaaaa aaatcccaac ttttttctgg ttttaatttt aaaattgcag agattactct173760ttagttttta aaaatcacaa aatagtgatt ggagaggaat gtcaaatcac ttaagtcagc173820ctcactacta tactatgcag acgtttttat gtaatgcatg gtagaattac ttattactca173880gctcacagga caacttgaat gtgtagatca cagaatgaat tcaggaatga acatcacaaa173940ttacacaaca ttgatgcatt ttaggtggaa agcaaggtaa tgaggaagat agttgagaaa174000ctgtgaaaaa aagatgggca gtacaccttg aacctggctg ctgtcaggca agtagagggc174060catttctacc cctgctttta cagagacact gctttttcca ggcttgcagg atggcagagt174120aaggtggagc tccttcagag agaatgagcc tgctcaggac ttcaaattat gcacaaccta174180
cattcgtgtt cttggtgatt ttaaattagc catagttaaa atttcaagtt tcctggtttt174240aaaaagtatt tttatactgt atttaaatat cagaaatctc ctgtgcttag gcatgttttt174300taaatgaatt tgtccttaac agtttctgag ggagacactc atgattatca ctgagttgga174360aatgaagata atgttttagc attttgtttg atacaagttt tgttcatttg gcttaatttt174420atattaattg ttgaagatgc tgatcagtgt ttggggtttt gtttatttgt ttttcattac174480tgttaggtgg cttcagccag attcatatgc ggatcctcag aaaacatctt tgatcctgaa174540taaggatgat attcgttgtg gttggcctac caccataact gttcaaacaa aagaccagta174600tggggatgtg gtacatgttc ccaatatgaa ggtaattata actggattaa attagcagac174660atctatatac tggctgcaat gactgataaa attttagaaa tgccaagtgc tgagagtcca174720tttgttctac cctctttata taaagggtga tgctgaaagt ttgtttaaat gacttgttta174780tattaattag tccccaagtg tccaagttac acctgttttt tttgtgagtt tgttctttac174840attttgctac ctgttacggg gactcaaagg agggataaga aagtatccat ctaaagagtg174900ctagacacat acagtgaagc ccctcaatat gtattgattg aataaatgca tgaaagaata174960catttttaaa ttttgtgtat agttttgaaa gactcaagta cgttctgtgt ttggtattac175020tgaaaccaca ttttaaaaat aacactcatt aagttagaaa tatatgagtt tagattgtaa175080aagaatgagg aattgaaata gttgtatacc atattgatga atatagagtt tttaggatac175140ctcttacctg aaatattaat aataatgttt cagagcatat tatacataat tatttgtgat175200ttaatctgtt aatatgaata tctcatttaa aacttttatt tctgaaaaaa ttatattgaa175260taaaatttta tataggcagt ccccagccct ttcctccttc aaagttgtct tatagagtga175320ttggttgttt gaagcttaga gctgattaag ccacaaaatc ctttttgctc attgggtagc175380taattttgcc tcaatattat tttcagtaga actatttcaa ctacttcatg cattctttca175440tttatagcgt gaagaagtac aatgccaatg tagcatctta cctaagtaaa aaataagaac175500acattgctta catgacttaa gaccagtaaa taaaactgta tggaaaaggt ttcaaccctg175560atactaaatc aaaatcagtc ttttagcagt atctttcaaa tatttggcca tgatccatag175620tcagaatttt tattataacc caaagatata aatataatat atgatggaat agtgtctatg175680taatagcata tgttaagtac atataatata ctttatatat acatacacac atacatacat175740aatatgctat actctgatgc tttctctatc ttatttttta aggcaagtca tacccttcac175800aaactgagtt catgcctact tggaattatt ctatacaatg caaaaagcat cacaaattat175860
aagaaaaaaa gatttaaaaa taaagttatc ctgatagtga tatgaaaaaa attactactc175920aaggacagat ttttgatcac agtacaatac acgttaagat gttgaagggc ttgtatattg175980tgtattcact gatgaatttc tcaatgtagt gagttgttaa atccaatctt tgaaccctca176040agattaagtt ttttattcat gtgacaacat tctaatacca tgttcatact gttacgcttc176100tgatatttct ttagttgtat tttctttctt gtgttttctc ctttgcacct tttattatct176160cttttgatat ctggtatgtt tatatcttgc atttgaaagt cagacttcat ctttttttct176220tttcatctta gagttctttt tttaaccata tgatggatct gctttttttt ttcctctctt176280cttccctctc ctctttccct ttcacctcct tctccttttt ccttcttttc ttcctcttct176340ttctcctcca tatatttgct tctcctacta tctttccttg acttaaacct cttactcagt176400gtgctctaaa ttcatgtcag atttatccaa tttagaaact ttctagaaaa ccactctaaa176460tactatctag aagaagtgat ttgtgctcct actgatgttt aatgtattaa tatcatatta176520tattagctgg aaacaaaatt aatttaacct attttacttt cagcctatta gtatatgaag176580tcttaggagc actttagact gttcataaat tgttaaaatc tttatgcagt aatagctaat176640tatttgaagc ataaaagttg atttgcagct aatatttcaa tgagttccaa aatgctttca176700aaagcaacaa gtgttattgt tataattttt tacttataaa atgactgttc cctacctgtg176760ataaaatgag ttagaactaa acacacatat tgtaccaatg tcaatttcct ggttttggtt176820cagtaccatc agtgtctgac agtgttggat aaaatgtagc cattggggga acctgagtga176880agggtacata ggacatttca tctatttttg aaacttcctg tgaattaaaa aaaaaagttt176940attttaagga aatcttattt attgacaaga gaaagtcaca taggaaatac aagaataact177000ttttttttaa gtaacacagt ttgtaaaaga aagcagaatg gttattctat aatgtctcag177060ttttcttaaa gtataaaatt attcagtgat ttacttcaag gttcatggtg catacacaca177120tagactctaa tgataattta ttaaatactt agggtaaata tagttgctta cagaaaggtt177180aaattgaaat agtatgtata aaaatagtgt ctgtaattta atatcaaata tgtttgagac177240aggagagttc ccttgacccc tttgtgggac ttgggacgtg ggtgtcgtgt ggctcattta177300cttgactgct gcactcaaac cccttgcagg atggcgagca ctgaggcaag caggtgccag177360ggcccaggca agcacctctg ggctccagcc ccacagcagc atctaggagt gtgtcacaat177420taatgctttt ttagctttgt tgtctgtgga tggctaagtg ttaaacagct cagtgaagaa177480tcagcatgac agcctttttg ggttcctgca cccagtgtgt cctgaattct tgtctggcat177540
tcaggaagaa tcaggtcata tgaatggttt gaaacgcgat gaatgcgtga atacagcaga177600tttttttttt tttttttttt ttttgagaca gagtctcgct ctgtcgccca ggctggagta177660cagtggcacg atttcagctc actgcaagct ccgcttcctg ggttcactcc attctcctgc177720ctcagcctcc cgagtagctg agactacagg cacccgccac cacgcctggc taattttttg177780tatttttagt agagacgggg tttcaccgtg ttagccagga tggtctcgat ctcctgacct177840tgtgattcgc ccgcctcaga ctccaaaagt gctgggatta caggcgtgag ccaccgcgcc177900cagccacggc ggattttatc aaacggtgga agtggctctc agcagaaggg gagctagaaa177960gggcatggtg tgagaagaag gtgatatttc cctgaagccc agccgtctcc agccatgctc178020ctctcctaag tggtgccatc tgaggttaag ccgcgcgtct catcagttgc tgcttctcct178080cttggtattc gccacttgtc tctttgctag ctgaagtctg ggatttatag gggcataggg178140taggggggca gggctggcca aaaaggcaac atttaggcgg aatacaagga taaactgttc178200tcatttaggg tcatggtttc caggcttgag agtggggcct ttgccaggga accaccttct178260cctatccagt atatccctga ctcctgtctg tatcatcttc agatttttta attgtttaaa178320gtttaaagct ttttatgctg gcacatgtgt ttgagttgaa attttaacca tatacctgta178380aactgccatt ttttaaatat aagaatgggt ttttaatatt tccaggggtt tgtgtcagtc178440caaatactga tctcttgtaa cctcacctga agatagtggt cacttaatgg aacatatttg178500tcacctgtgc ctaacattat ataaatagcg aaagtctcaa actactgatt ccatagaatc178560gcgttgttaa gaacaataaa cataaagaca gtctgtagtt ctgaggtagc tctcaattac178620ttaaaaatac tatcactttt atctctgcac tttttctatt tctgtgacaa ttctgttgaa178680ttacctgttc ttgccaacca caaaactatt gtctgtaggg agcagcagaa actttgagta178740aagctcagaa tggtgatacc tgctgaagaa ctaaagtggt attcaaattg tgtgtacatt178800tgtggttgcc taggggtcaa gtgtttttct gccaatgcat aaacaaataa aaacaatgtt178860ttcttataat ttctgggaaa tatttctaaa aagtattttt cagaaatagt agttagtaga178920tgaaatagta gagttagtag atcaaagata gtcccgtttt gttgaagaaa ttgagttgca178980aagttataat tcctggaaaa tatatctaaa aggtattttt ataagacaat ttgtattttc179040agtatattaa catccattaa cgaatctgtt ttttgcatct atactgtaga gttagtggac179100gaaagatagt cccattttat tcttaaagaa agtgagttgc aaagtttaat tatttaatag179160attgcttaaa tcaaatgttg atgttggtga tagggctgtc taataatatt ctttcttcac179220
ccgagtaaag aaacatattt gggctttagg tataagattt gttttattaa gtgttaattt179280ttgaaacttc tataaataga gtacattttc ttctgtagaa cagtgcagct caaactgaca179340ttagtgcttt aaaatgtgct gagattgtgg gaaagcattt acacttgcat tacctgataa179400aataatttgt tagtccctgc aataacttaa tgtagattca ttccactatc ttttcctgta179460ctttattagt ggtgcatttg tttttatgtt ttgatatctc tgattatgta ttccaaaact179520atgaggcctt gtttttacac cgtgttcatg gttcatattg tcaacccatc ttgaggtcat179580ttgaactctt ttcaataagc ctttgaaggg aatgccagag acagaccaat gctaacataa179640agtatacata attatgagtt tataatagta agttgcttat tttgatgtca gtgacaatta179700aataataatg gtatttggtt tctataatac catgtttagg tggaagtgaa agctgtccct179760gtttctcaga aaaaaatgtc tttacaacaa gatcaagcaa agaaacctca aaggattcct179820ggcagtcctg cagtaacagc tgcatcttct aatactgaca tgacttatgg agggctggca179880tcaccaaagc tagatgtttc atatgaacca atgatagtga aggaagctcg atatattgcc179940ataacaatga tgaaggtaat ttattttact tatcagaaaa attttagttt tcagcatttg180000tttatttaac caacatttca gtgattcaga tgcagacatg aaattgtaag tccaaagagc180060atttgatgga tattaaattt gcagcaacac ttattaagat gatgttatcc tgtcttcaga180120gaggattttc attggttttt ggcagatgtc cagaggggat caccaattac agatcaagtt180180aatcgaatca gagatacaga tggattaggc tgagtttcag ttcctgtgaa agtacttgtc180240tacttctgac caggaacagt ggcccacacc tataatccca gggctttggg aggccaagga180300gggaacatca cttgaggcca agagttcgag accagcctca gtaatgtagt gagaccccat180360ctctacaaaa aaataaaaat aaaataaaat ttctaagtac ttgtctattt gcagtttact180420attcttgcta gaatgtatct cttcagggtt ttggggttta cctatgcccc cttcaatttt180480gggttctctc aaatgccaga tgtatctcct agaactcttt gggattttta gctctctaat180540acctttagac atttaaaaaa tatatatttt ggatgtttta gttatcttca gaggcaatgt180600taatccgaat tatcaaggta gtcattattt gaagctgaag tttgtaattt tggcattttc180660agagagcagt ggtgagccat tgaaggcttt tgggtagagg aatggcttga ttagatttgt180720gttttgtaac gattgtggtg gaatatggac agtgtaattg ggcagatgat caagaaacat180780gacttggttt tagtctcttt aggggactat tgacttaatt tggtgagata tgctgatcag180840ttggactagt gagttgtcaa tgaggataga ttcaatagaa tcagtagaac ctggtagcta180900
gtggaggtgg agtaaagaag aaaggtagga aataaaagat gaaattcaca tattgagctt180960gtgtccaatt gataatggaa gaggggcagg ttttggttga gaaagatagc agattcagtt181020ttggatgagg tgagttttag atacttctga aatacctacg tgaagatgta taaaacaaag181080ttagatgtac agacctgagt gatcacagtc tgacctcaag aaaaggattc aggaatcatt181140agcacttaca ttacttatga agtcatggga atagatgata ccttctatgg tgaatgaaga181200cagaagttcc aggataggat cctaagaaac accaatatca ctgagtctca gactcttctc181260tacatcagaa gcatctggag cacttactta aaaaaaaaaa aagatttcca agcacccaga181320gatttagatt cagtgggttt ggagagaggt tcaggaattt acctgaaaca aatatgcagt181380gattcaaatt caaaacctaa actttgagaa atactgattt acaaggtaga taagagaaaa181440agaaaggaaa aggaaaaacg attcacattg gtaagaatac tagccaaaga agcaacacat181500aattaaggga atggctgaag tgagtgtaga taagcctttc aagatgtttg actatagaaa181560gaaccagagg aaatggaatt gagtgaaggc tttggagtat ggggtcttat taatagagat181620agagttacat atttgagtgc tgattaggag gtaattgaga gaaaaaacaa taaagatact181680ccgaatataa aaatagaatc tagagctcaa gtggtagcat tggccttaaa taggaggagg181740agtagtgctt gtttcattct accagaagaa ggaaaagaaa gcttaataca gatgctagta181800aatttgtagg ttttgcagcc aaaggaatta tttaaaacag taattatgtg ttttgggatg181860gaaatggaag tgatgaatct tagggtcaag gttttgaaac aaagcggata aaatgaaatt181920gttattctag agattaaaag aaagcaccga tcagaaaaga tttattagat gcctttgaat181980aatcagttga aattgaagat tatttgtgaa taacagtgtg agtcatatga gcatggataa182040aacaatcatt tggatggatc tagcattaaa attttgctag accagcaaaa gaacagtgga182100acaaggaagt taagtatatg gtagctaaag agaggacagt ggaacctaat ttagtttgga182160agggaagaaa tttcagagtc aaggaagtaa gagggactag acgtcaaagt agatataaga182220atagttattg aggtaaaaaa ctgaacaggc cattttattt tgatttcaga gacagaacaa182280ttctgagtaa tgtcacaggt ctgtgacttt tggtgtgagt ggctggaaaa aaagttgaag182340ttattgaggt taaagaaatc agggaatgag aaactagagt tctaggtgaa ttgtccactg182400gctgttaaag aattcctgga aaaaaagttg aagttattga ggttaaagaa atcagggaat182460gagaaactag agttctaggt gaattgtcca ctggctgtta aagaattctc ttctctcact182520ttttactcac caaattaata atctcattat gaattctata tccgaaataa atatttgtct182580
cattttttaa aaaatccatc ttacacattt ggctatttta gtatcaaatt ttaaaattat 182640ttctactatt aaaaaaaaaa gcaaggctaa aaattcttct ctattccaac acttgcttaa 182700ggaatttagt ttctagtctc cttacctccc taatctttta gtttcctatg aacatgcttc 182760agtttgtata atttcttttg caaattttgt taccacaacg gaatgcaata tttcaaacat 182820gttctgagta gcctaggttt agagcaggta tggaatacca ttcccaaaca gtatctgtga 182880aatgttaatt caggtcccag gaaaggaaat tttccttatc aaccattcac agatactatg 182940tattcactca gtagtgagcc aagtattgta gaggatggaa agatgaaata tggtctcttc 183000tttcattact aagtgtgtta ataatatgaa tttgagggag agtgatgaaa tagaaaatac 183060attgtactga aagtctagaa acatggattt ccttttctca cctttgtatg gctaaagact 183120cagccttaac ttgcgttttt ctcatgtgta acttttgagt aggtaaagtc agctagatca 183180tttctaattc atttttatga aaacaaaaag ggtagagaga aggaaggggg ctttgagttt 183240gcttcctttt actgtaagta caggaggagt tagaagtaga tgagcagttg gacatcttga 183300cctgaacctt gaaagataat gatttctttg ctaatgttgc aggcatacag aagtattcag 183360aataatgtgc caagtttcat gtacctacag cccatttttg tctttacatt ttaccataat 183420tgtttcaggt cattttgtta tttttttatt aagaaacaga ttgctacagg tacaattgaa 183480gcctgcctaa acccttcctc agacccatta ccaccccttc cttagaggta accactgccc 183540tgagtgtagt gattctcatt cctgtatata tttttaggct cttactgaat acaaatttat 183600tcataagcag tatattttgg gttgttcaat acgttttcca gaagtttata aaataatatc 183660gtattgtatg tatcattttg ccagattttt gcttaatgtt gtttttgagg ttcagccgtg 183720tagctatagg tcattcattt taactgcttt aaagcattcc attggggatc tattccataa 183780tctgtccatt ctcccattga tggacgctta ttataaactg tactgaaata aacattctgt 183840atttgtctcc ttttgtacat gagcaagagt ttctgtaaga tacatactta gaagtggaat 183900tgttaagttg tagggcatgc acaactacaa ctacattaag tattgccaaa actcacactc 183960ccacgagcaa tgtatgaaag tagcaggtgc tgcatgttct cctctgcgct tggttcttac 184020caacacaatt tgatgaccat gatgtgattt attttagttt tgttttaatt cgtattttcc 184080tccaagttta gttaaataac ttttctagtc tttattgacc tttcagattt cttcttcaga 184140gaattgtctt gactgtcttt ttttgttgtt gtttatctct tttgtatgga tttattctct 184200atatgttatg gatgcatatc acctttgggt tatacgagtt acaaatctcc ttctccaagg 184260
tggaagcttt tcctttctgt atagtatttg ttttggaaaa ctttaaaatt ttaatgtact 184320caaaagatgt tatctgtttt tttcttttgc tttttttttt taagttttgg tgtttacttt 184380tggatcttca aaccttgtag aatttatctt taataaatgg tctaaagtag gtaatttttt 184440ttctttttct cctatatgta taaccagttg tctcacaacc atttattgaa taatatatta 184500ctctgttaat tgagaatgtt tttaaatgat tttacgattg ttcagactgg tctctttctt 184560aaaggtttat gaaaattatt catttgaaga actacgtttt gcatcaccaa ctcctaagag 184620gtaaggatcg ttttctcaaa gtataaccaa aacatgtgtt gtcagctgct tgcaactaaa 184680atcatcctgc atttcaactt tattttctat aaataaatac tttatagcat ttttctaggt 184740tgccgataga gatgtcatat aataatgtat agatttgcag aaagaatgtg tttacattca 184800actgtaatac tgccaatata ttacataata ctacctacta gtgaaaatag gtatgtaaag 184860tagatatcta gtatgtaaaa atgtttaaat tgacttgttt agggttgatt agtttgtttt 184920ttgtttgttt gctatttaaa aattgttcct tttctccttc caatgaattg tggagagatt 184980tcagcatgtg gtatacttgt atttgattgt actgtcttaa tttttcagcc cgtaagtaat 185040tctgttaact tcagatttaa ggactttttt tgagttaata ttttatttgt taggaacagt 185100actacttaaa atagaaactt ggaaaaaata attagatgca ttatactttg gagagatcac 185160ctccatagaa tttagaaaaa tctctgaagt gaatctgaga tagcagaatt atgtataggc 185220tggaaatacc agtttgaaac tcatggggaa aataaatttg ggaatcatct atattgagat 185280ggcatttaaa actgaggaat ggttgtcacc tagatagatg gcataggtag aaaaaagatg 185340acccagaccc aaatcctggg gcattcctta aattaaacgg tagtcagaaa agggaaagca 185400acaaaacact gacaaagaat ggccagtgag ataagaaaaa agccaggaaa atgtcacaga 185460agcaaaaaga aggaagtatt tcaagatagg aagagttgtc agctatcaga tgctactgag 185520agattgctga ggatgaagtg accattgaac ttggggacgt ggtggtcatt ggtgactttg 185580acatcagcag ttgtaaggaa atgttggaaa caaaagccta ataaggataa attgtggaaa 185640ggaagtagaa gtgagagaat ggagtttgat ataaatcgga gaagagatgt aagaccttat 185700tagagggggt tgtaatacca aagggaggtg ttagtgaaga ttctaaagtt tgtgtgctaa 185760tcgaaatgat tcactaaatt gagagtaatt gatgatgcat gagagagaac aaggtgattg 185820cagcataaaa atgttgaggg aataagattc acagcacaag gggagggact agctttggat 185880cagatccagg acacttcctc cattctacca tgagagaagg caggggaaag taggtttatt 185940
aatcacattg caactttgag gttgtatctg attgcttatt gtctcacaga agaaagaggt 186000gaggtcatga tcacctgaga gttaggaaga gaggtgatgc aggtttgagg taagaaagaa 186060gtgaaattca agcattttgg agagtaggca agcgtactat ggcagggtgg taggattgcc 186120aggaggtatg caaacaaatt aaatgtttgc tgaactatcg taatgatgtt gactgcttaa 186180ccaaaagtaa aatttccaat atccatctta tttcttttca gacccagtga gaatatgctg 186240atccgtgtca ataatgatgg gacttattgt gcaaattgga ctccaggggc tattggactc 186300tacactcttc atgttaccat tgatggcatt gaaatcggta ttttctttaa agccatgagc 186360tactacttac ttcaaataac tagagtcaaa attattttta tcttctagtt ttgcccatta 186420acttgcacta gacatatgga taggtttcat aacttatttg aggccttgtt ttctctttca 186480taatgagtat ttaattagtg cattaacatt ttgtaataat ttctggtgtc cgcccaggca 186540ctgtagccct catgactctc tccctccatt tcctgatgtt actagtcagg tgtgctctct 186600tccacgctgc tgttaccctg tctctcccct gtgggtgtgt ctgtgcctct cctgactgtg 186660agcagaaagc attcatgttt ggatcttcca gtgtagctca gtgcctggca cataatgaca 186720gcaaatatgg atgaatgaat gcatttttaa atcgatcatt gtgttaatct gggttctcca 186780gagagacaga accaatagga tatctataca gtcatgtacc tcatagcatt ttggtcagtg 186840acgaacagcg tatataatgg tggtcctata agattttcct gtaccttttc tatgcttaga 186900tatgtttaga tatgcaaatc cttaccattg tgttacagtt acctgcagta ttcagtaggg 186960taacatgctg tacaggattg tagcctaaga ccaataggct gtaccatata acccaggtaa 187020tctgtaccat ctaggtttgc ttaagtccac tctgatgttt gcacaatgat gaaattgcct 187080ggtgattcat ttctcagaac atttccccat ccttaagcaa tgcatgtcta tattagatat 187140atgagaggat ttattaggga aattggctcg cacaattatg gagtctgaaa agtcccatta 187200tgggccacac ctgcaagctg gagatcctga gatgcaggta gcctcagaac cagggaagca 187260gatgatgtaa ctctcagtct gaggccaaag gtctgagaat ccaggagggc cactaataat 187320aagtcctgga gtctaaatgc tggggaccct ggagttcaaa tgtcaaggga cagtagagga 187380agtgtttatc caagctccaa aaaatagaaa tacattcacc tctcctctgt ttttgtttgc 187440tctgtgtccc cagctgattg gacagcgcct gtccacattg aaagcagatc ttctccacct 187500actccactca cactcacacg ccagtcttgt ctggaaacac cctcacagac gcaaaagtaa 187560tgccttacca ggtttctagg tattctttaa tccagtcaag tggacaccta gaagtaatca 187620
tcacaaccat taactttgaa aaacatattt cttttgtttt aggcattaat cttttcttta 187680cttttaaact gtctgtataa taggataaca aggagaaaac tctagtactt atcagtgtga 187740atttcttgta acattatttt tcctgatgct cctcaaggaa aggggtttct gtgatcagat 187800aaatttcgaa aacctggttt gggatgagta tcttttatct gaaatgcttg gcaccagaag 187860tattctagat tttggacttt ttctgatttt ggaatagttg tattacagtt accagttgag 187920catccctaat tcaaaatttc aaaattcaga atgctccagt gagcactttt tttgagcatc 187980atgtcagcac ccaaaggttt tagattttag atcattttgg atttggggtt ttcagattag 188040agatgctcaa cttgtatatg gtagtctcac ttgagacatt cacacaatct acattaacat 188100attagggatt ctgagaagtt ctgaaatcaa gaaacttgtc tgactatgtt taatccttat 188160cgtgggaccc cttgcaagtg gagaagagag aaacagggtg gagatagtaa taataccaat 188220taatatttca ccaaaatatt tgggaaaccc tgctttaagt agtttgcttt ggcagtggag 188280gtaaagaggt actttacatt gatgtaattg tatgttctca tacattaatg taactatata 188340gactcagata tatccagttc atctgtacca ttctagagac cttcaccagc agcctttgct 188400gcactcttct ttatttcaat atattcataa agcctttagt tcagcaaatg ttttttggtt 188460ctgagcatac attttcctgg gtaagacaat tcctgtcaag tgttaacact ttaatgagga 188520ttgtgaaaat aaaacacgca aacaaaaatc ttatgaagta gaatataagt agtgacacac 188580aagacataca aattacagta ggaattcgaa agtagaaata gccaagaatg acattgtgaa 188640aattttcttt tctctaaaaa ttctggtaaa atttcagtag atagagtttt tttcatcatc 188700ttatctaata gtggggatga agaaaactga tacaaattag ataagaaaaa tccacttata 188760tttgtttttt ggaatgaagc tgaacaaaaa tacttgctta cttagaatca tccattttct 188820tccatactca acttccctgc tcttcttcct aagttttctc cgatgccatc aatgggctgc 188880gaccttgctt gaccatagac aagcagagat aataattcac tagaatagtt ataataataa 188940tagctagcat ttattgtgca tttaatatat atctaaattt tctctatgta tacttttttt 189000tttttttttt tgagacagag tctcactctg ttgcacaggc tggaatgcag tggcgcgatc 189060cctgcccact gcaacctcca cctcacaggt tcaagcaatt ctcatgcttc agcttcctga 189120gtacctagaa ttacaggtgc gcgccaccag gcccggctaa tttttgtatt tttagtagag 189180atggggtttc actatggtgg ccaggctgat cttgaactcc cgacctcagg tgatccgcct 189240gccccggcct cccaaagtgc tgggattaca attgtgaacc actgcgcctg gccccttgta 189300
cattttacgt catttatttc ctacaacagc tcggtgagtt agatactgtt atttttccca 189360ttttatagat gaagaaattg aggcacagag agtcaaacag ttagtgcgag atattggagt 189420aaccaaagca gtctaacatg agaacctcag cttctcacaa ccatgctaca ttgtcataga 189480gaggactggt agagatgatg agtggtattc tgattggata ttgtcagata agatttaatt 189540ctcaagggtt ctttttttta ttattttcca ttttttctgt atcatttcat atgttctagt 189600taaagatagg gagccccttc ttttccttga catcctgaga cattccttta gaatctcagg 189660gccattttta aaatatttct cttgtttagc ctattaaata tccatcacct taaaaggaca 189720gaaatcagat atttgtgtta atttttaaaa atagcctgtg gatgtttata agattaggtt 189780taaggatctt ccttctcttt ttttctcaaa tgttacagtt ctctgatttt tctgacagat 189840gctggtctgg aagtaaaagt aaaagaccca ccaaaaggga tgataccacc aggaactcag 189900ttggtcaaac caaagtctga acctcagcct aataaggtta gggcagaatg aattgagagc 189960cggaatcatt caatttttac gtttgtcact gatgcttcaa aatggccagt aaacatctaa 190020cattttactt gatttcagga cttgaattct aaatatccct aaataaattt agagagtagt 190080tcatggtaat atttctaagt tgtagcatgt gtacttcttt atttggtaca tattgtattc 190140ttcctgctgt ggaacattta caatgaacaa aagtctactg aaattaagtg taaagtttgc 190200aaggtgaaaa tgtcctctac tgtcaaaaga aaagttaatt tctgaaatct aaagagtaag 190260tacaggcatt ttggtaaact gcatgttaaa aaaatgaaat attggtaatt gaatttattt 190320ttctaacaga aagaaatggt ttttatcctt cttaacacgt atcttcagta tttagaaatg 190380agttaaaata tatataattg tctaagcttt attttcagta atacacattt cagtgcttaa 190440aagttctgtt gttagtgttg ttatactgtt atttgcagag ggagcctctc tacaaataat 190500tctggaacct tattaagaat tacgatactt tcaaacctgc ttaattttac ttcttgctac 190560ttgtaagatc tctattctat gttcttccta cctcactgtt tatctttgcc tttctcaggt 190620tcgaaaattt gtggccaagg acagtgcggg gcttcgcatc cgtagccacc cttcccttca 190680gagtgagcag ataggcatag tgaaagtcaa tggaactatc acttttattg atgaggtaat 190740tgataaagga gcttttggta gtaaattttg gacagaaagt ttttgcattg tttgcataat 190800aataaggtta ctgagttttt tgtcagaata tgttttaagc agtttgggaa atattttaaa 190860actattttat agaatgtgtt cattgagttt caaacatttc atttatttta tcattaaaaa 190920atgactaaaa ttatattttg aacatgaaaa gaacaatgtt cgacatagta ttcagaacct 190980
gtacatgtat tcactgaaaa tgggtatagt tcttcatgac attttaaaat gttgtttgtt 191040ttgaaatgtg tttcttactg ctttaacttt ggacagtata tttttctggt aatataacca 191100agtaacttct gtttcttaaa atataaatat aagggaagga agactcatat tcctttggga 191160aacatctgat tttactgttt atgtatctta aagtgtggtt tccttgtgtt tttattccta 191220agtgaaattg tttttacagt cttgccacat gaattaccat attgtgttgg cactctttgc 191280tgcttttttt ttccctggag ggtctctcag acttactttc tgcaaaaact agatgcttaa 191340tctgcacatg caccatcatt tttctgcttt gcagactttc agaccttgct agcctcatag 191400gacttacagg aaggcctggc ttgtttctgt tgcttttttc tatagatcca taatgatgat 191460ggtgtgtggc tgaggctgaa tgatgagaca ataaagaagt atgtccctaa catgaatggt 191520tacactgaag cctggtgcct ctcttttaat caacatcttg gcaagagtct tctggtccct 191580gttgacgtaa gtaaaaggtg gttttaaaaa gcattataga tacacgctgt ttgttttaca 191640gagctttctt taattaaggc ttttcagttc tgaggtaacc cacaactaag taacctttac 191700tttacaattt cttgaattct taagggcttc tgtgtaatga aagtgaagtt tggcaaggtt 191760aaggccctta gtgcagatta tgatccatca cagataggca ggaagtagta aatttaatta 191820attgctactt aattaaaact actgaattcc tttcaaagag gtagaaaatg agagtggtta 191880catttttttc tttcttattt atacagctgt gtggaatgaa ttccaaacta aagtttatga 191940actcagaatt atacattcaa aaacatgtat aaatgtatgt tatgttcaca ttttgcagat 192000agttctgtgt gttctgtcta attttgactc tcttccttct cggcttataa tactattatt 192060tagatttgtg gtgctccgtt gtattctagt tgaagttaaa tcctagtctt tgagagaatt 192120cattgtacct ccttgttgag tatagtagaa tagagccagt gttcacaaga ttgctacttc 192180atgaagtata ataataaccc acacttcgtt ttatatatta accaggaaag aatgattttt 192240ccttttttca ttaccatcaa atgtgtgtgc tctttaggat agagctaata aatagcacgg 192300gtaaaatttt atttaaaaac ttagtatttt aaacagttga atttcttaaa aatccgttta 192360taaagttagc ttattgtatc aaaccattca gtatattcca tttgactttc tccttgattg 192420tccataataa tgttctccaa agcagtagtt tatataatac aagacaattc tttggtgaag 192480aaaacattgg aatttctatt tctatttatt gatctcattc ttttttatat gcttttgtgt 192540atgttttata atagcacagt ggagactgta tatagacaga gagcaagaaa gagaaggaag 192600atatgtgatc aagaagattt agagtgttgg cttactgctt tacggaggtt aagttgaaca 192660
atgatacatc tataagtaaa atactaaatt ttaaattaaa aaattacatc aattacgtct 192720tattaaattt tcctcagatt tatacctgca gttaaaaata aaaaggattt tgaccctcta 192780tgaaagtgta ttttaaagag ataattatag caaccacttt tgtacagttt ttttttccaa 192840tatagacttt tttgttgggc aagggggagt tggtttttgt tttttataca gatgggagag 192900ccaggcgagg tggctcacgc ctgtaatccc agcacttttg gaagccaagg ccagtggatc 192960acctgaggtc aggagaccag cctggccaac atggtgaaac cccgtctcta ctaaaaatgc 193020aaaaaattag ccaggtgtgg tggcgggcac ctgtaatcct agctactctg gaggctgaag 193080cgggagaatc gctggaactc aggaggtgga ggttgcagtg attcaagatc acgccactgc 193140actccagcct gggagacaga gcgaatctct gtctcaaaaa aaacacaaaa atatagagat 193200ggggtctcac tatgttgccc aggctggtct caaactcctg agctcaagcg atcatcttac 193260cttggcctca caaagtgctc atattacagg catgagctac cacacctggc cattgtgtgt 193320tttatttaag aaaattttct taagtcccta ataaaatgtt attccagatt atattatctt 193380ttctaccttc ctagtccaaa ttttctctga tttctttgct catttgagga gctgcttttg 193440ggagagaaag atactcacac agataaagca agtcaataaa caaaagtaga aaggaagagc 193500aagaagtacc atctgtcaca attctgaatc ccacgatttt attttaaagc attgtgttct 193560gttacttgat gcttttgaac cccatccaag agacaatctg tagtagacag tctccttaag 193620gtcagggaaa gaagggagta acaaaggtta gaggagcggt aaggagtgga gtgaggcata 193680cagatacttg tgggtggttt tcactggaat gtgagtgcgg aggttgttaa tgtaaacagc 193740tcaagagtgt ctggtattgt taggaagcta gggttttttt ggtttcttca ttttaagagt 193800agtagtgact tgagagtttt ataagttaaa agaaagatac tagtagaaaa agggagaaat 193860taaagatata gatgacaaga ctgaagactg agaaacagga ggaggaaatg gaattaaggg 193920caccttagag agcttgcttc tgtaaaagag aggggaatat gcatggcagt agttatgttt 193980tagaggaggg agggacgaag ggaagaggtt gagggagact acaagttccc ttaaaataga 194040agataggaaa acgatcagtt cagaatcaaa agaatgagtt aagttagggg gttgagaaat 194100gcaatgaata tttgaaatat tgtttgaagg attactgggc cactaaagac tcaatgagac 194160cactgtatag acccaactgg aatcccctgc ctagtccttt attgccctaa gtgtctggaa 194220ttaaaagagc caagaaagag ccaagaaagc aaattatagt actagttgct gagctgctgt 194280gacagaaata gcaattaaga ttatcagttc tgggaatgtg gactcagacc taagttagaa 194340
atgtgaaagg acaaatggaa ggtccaaggg atttagaaga tttaataaaa tcctagaaca 194400atatagtgaa tgcactatag ttaaagagtt ggaaaaatgc aggcaataaa gtagaatatt 194460tgtatttgat atcataggta gggcacttta acaaatgaga tactctatta cattgccatg 194520ggagggtggc tgaagtgcag tgcacgtgga agtcactaca agtgcctaag tctaggaact 194580aagaagttag gcaggtggaa gatcatctgc atggacttga agacacacag atgatgtgac 194640tcacaaagtt aaagataaaa acaggctggg cacggtggct cacgcctatt atgccagcac 194700tttgggaggc caagacgggt ggatcacaag gttagcagtt caagatggtg aaatcctgtc 194760tctactaaaa atacaaaaat tagccaggcg tggtggtgga cgcctatagt cccagctact 194820caggaggctg aggcagagaa ttgcttgaac ccaggaggca gaggttgcag tgagctgaga 194880tcacaccact gcactccagc ctgggtgaca gagtgagact ccgtctcaaa aaaaaaaaag 194940aaaaaaaaga taaaaacaat gtgtttacca ttggaggaag gggtccataa gacacaattt 195000aaagtcatgg ggggatggga atagtttgga tcaggaagaa acagagcagt ttggtataaa 195060aatgaaagaa caggtagatg acataaggat ttatgttttg ggtggtgatc aaagatgaca 195120gatggggtac agtatggcaa aattaattgg catagggctc taaacagatc tgggataaag 195180ttacttcagt acaggtatta actgaggact catacagagt cagaggcctg gcataggcag 195240ggacaggtat gattttctgt agggactagg gcagacccct tacgcagtca gtacattttt 195300tttatgagta tctgactgtg cagggctaaa catggtagga catagaataa tgattccctc 195360tcatcttctc aaggaaataa cacacctaaa tagttgcatc ttgctgcctc ttcttttgta 195420tgtcttatac atttactgct ggaaaaacta tctttctccc ttcttacttc agttcgttta 195480aagtaaatga actttaatca tggctgttaa ttgtaaaaca tgactcaata aaaaaatgaa 195540agctgggaga tatgaaatca aattagaagt attcaaacac caatgctgct aacttaaaaa 195600gaagtgaaag tactgaaaag ttgccttcag cactgtttgt gtctatctct tcaacctttt 195660ctagttaaaa caattttgta ttgacatcaa tgtgtgcacg ttatttggat cccaactcaa 195720acaaactata gagagaggaa tgggggcaga gattggaagt ttgaacactg actgaatgtt 195780ggatgaatta tttttaaaaa taagattact ataattaccc attttagata atccttaaag 195840tgatcttgtc atgctgtctt gtttactcat gttttagaat tcgtgtattc atcgttaagt 195900atgaacttta gctcaatttg gatctatcag tctttctatt taaacaccat atttgactga 195960aaaatattca tatcctaatt tagttcaaag tgactttaac tgtttttagt aatgctctag 196020
ttgagagaca aaataacatt agacattcat attatgactg tatcgtttcc aaaataattt 196080ttaagtcaat gggaaagata gagctaaata gatgaagggt tcagatcctc tgtgaggcat 196140tcattatcaa ttatgtagaa ctttttgccc tttttagaac acttttcctt tttttttttg 196200ttatttttgt tgttgttttt tgttttgttt tttgtttgtt tgtttgtttt tacagcacct 196260tgccctgcca cccaagctgg agttcagtgg cacgatcttg gctcactgca acctccacct 196320cccaggttca agcgattctt atgtctcagc ctcccaagta gctggtatta caggcataca 196380ccaccacacc cagctaattt ttgtattttt agtagagacg ggcagggttt taccatgttg 196440gccaggctgc tctcgaactc ctggcctcaa gtgatccacc taccttggct tcccacagtg 196500ctgggattac agactgggat taaagacgtg agccactgca cccggctgag aacatgcttt 196560ccagttttat gtcttaagag tctttgtttt gttgtctaat ttatctagaa tggtttcact 196620tccatttttt tctaatctta agttgtccag actcaaaaat tggttggtga tcttctcttt 196680attttccatt tttgctatag tcattaattc attaattgct catttagtaa atatttgagt 196740tcttattcgt agaattctta tttgcattct tgttagtaat aaatactaag ctggaaagta 196800ggcattgggt ggagatgcag aggggaggga tacagagtaa ataacatggg gtgaagttct 196860gatccttgga gtactcacca tgtaatgtgg tagacatacc atattcacat aattaaattg 196920cattgtgata agttgtggaa tggcttcact taagtgacat ttgaggagga cttgaagaaa 196980agttgccagg aaacagtaaa ggaatttcta gacagagaat caaatgttca gagttatggg 197040gacatgctat gtatgtgaac taatgaatag ttgatatagg taaagcagag aattgagggt 197100ggaaaacaga tatgtactgg ggagaatagc tttctctctt ccttctcagg cagcacttaa 197160tctgtagaag tagagaaggc ttacttcttt ctccagctac ccataatatt tgtttttctt 197220gccttcagcc catttgtgtt tatgtacctc ttatttctgt ttttaagcta cttaaggata 197280aggactcatt caggatccct tctcactcac atttgtttct tcctaagcat taaaaattac 197340actttgctca tagtaggttt tcacagtttg ttgatgcttt acacatttgt ggatgcttaa 197400ttttagttct ttgcatttga tatgatcatt ctgtttaaaa taatttctaa catatatatg 197460ctccttgcct taattatctc atttaatcct cattgtgacc ctgtgatgtt aagcatatta 197520taattctcat ttttgcaatg agtaaagata gccactcacc caggatcaca agtagaaagt 197580agtggagctg gtattggaac accagttgat atgattccaa agcccatgca tttatgttcc 197640gtatgcatta atctagtgga gagataagga taaatatagt tataaaagga tgaagcaact 197700
atagctgtaa taatcctgtt actttgacta gaactgccaa actacagaaa gataaatact 197760tataacctta atggttgaat gaggatgaat accatatgta ttgaagatga catgagttat 197820tctttgattg catacctggg gaacattgat tgattttaaa ttaaaattct gaagatgtgg 197880aatctgcaaa atgtgagcta gtagaatgtt tcaaatatca gggacaatgc aagtaaaaag 197940atgaagagag aatttattat gcacctacca aggaagacat tagactctgg aatcacaagc 198000ttaatttttt ggaaaacaag tgtacttcta tgataggaga tgttaatggt ttcaaactta 198060cacacccaat tctttgggta ctctgtcaaa aatgagatct tttaacattg tgaggggggt 198120tgagtggaag agaaagttta tatcaaggga agtagatgat agacaaatgg aagtattctg 198180aggtatcttt atagttccca tataatctga attcttacag aattgagttt ctgtatatct 198240ccatgcattt atacagatta cttcttattt taggctgatg ggtgttcaca tatagaaagt 198300atactgattt tccaaatttt ggaacctgaa agatttgaag tagagttgaa gagaaagtat 198360catttgttaa tcttgtttgt atagcttcac ctgggttaat tgataaaatt ggcaaatgtt 198420tcttgcattt taaatccaat ttcattcctt tttacctaat aacattaaat ccaatttcat 198480tcctttttgc ctaataacac ttagcagtca cagctttaac ataccccaga tttcctgatt 198540ttgttaatag ctttgtgtgt atgtgtatac ataacccgtg ttttaatctc aataatatta 198600acttaataca acattagtaa atgtgctgtt cttatatggt taagaacagc tttatttcat 198660tctttagctt agtgttaaag tttcgtaaac agaatgagat taaaacagtt aaattaagat 198720tgtaataata tatatacgct attcttaatt ttcctgagtc atttcatcat tttgctttaa 198780caatggcaat tcttagcttt gtgcttatct aacacaagga aagatttttt tcttttattt 198840gcatgagcat ttggcatggt tggtcactta ttttcactgt gataattatt cacttgttgg 198900tctcttttct acgccatgaa agattatcag cttagagttg caatttggtg ttcattaatt 198960tagttagatt cccagtatgc tttaattttc taattttctt gacagaactt ttctgccttt 199020aaaatagtat ttgagaatga aatgttattt tatatatatg tgtgttcgta tatacacatg 199080cacacacaag ctgtgcttat aataatctgt gaaattatac tatatgaaag ctaacatatt 199140ttaatagtga gaaattgggg ctttggaaaa attaacattt tcctgaatgt tcatatcttt 199200agactaaaac atttctaaat ttccaaagaa cactattcaa gtgtgtgtgt gtgtgtgtgt 199260gtgtttgttt gtgtgtgtgt gtgtgtgtgt gtgtttgaga tgaagtcttg ttgttgccca 199320agctggactt gaacttctgg gctcaaggag tcctgccttc tcagcttccc acgtagctgt 199380
gactacaggc atgtactacc atgccgagct atatttttaa attattttgt agagacgagg 199440tctctctgtc ttatccaggc tggtctcgaa ctcctggatt caagcaatcc tcctgtctca 199500gccttctgaa tagctacgag tataggcatg cactactcac ccagctattc aagtatattt 199560tatttttaaa ctccagcttt ctgttagtca aatttctgct tcttgataaa tgcagtttta 199620ttaggacatt gcattttgaa aataatctta gggtaaacca gtgtgaatta agaattttct 199680acaactgcag aacaaaagca aaaaagtata aagtttcaat ttacatggaa gcatatacag 199740aattgatttt attggcaatt tattaccaca gttagcagtt aacatcattt taaaatatca 199800ttgtacatgt tgttgactga gaaagcactt atgtttgcac attgacttaa aagagagaca 199860taagctttaa atattgtcaa ccagtcatca cattgcttct ttatcactaa ctttacctgc 199920attactttta acaattgcct ttcatgttac tttaatacta gaataattta tgctaaaaaa 199980tagaatacgc taaaaaaaag tgatattgtt ttatgtcaaa aagtctgtcc cactatgctt 200040tgtaagggag aaaaacccat tgaccaagta ctctacaaat tcttatatac gtggatgaac 200100cagtatagac atttttcccc taaaacatag cacttcaaaa tgctctgcaa tatgtttttc 200160actttaccta aagagaaagg acgtattccc tatccacttt taattagggg gcagttggct 200220ttggttttgg tttacattta tttgtaggca tattattaga cactttgttt agagttttat 200280atgtcttata tttatgaatt agtatatatt tatgtagatg ggtgtgtacc cacattcatc 200340tgtacatata tatgtatata tccatatata tatatggata tggaaaatat agaactagaa 200400aacagtaaat aaagtctcat tatctaaaat gagataatga gaaaagtttc attatctaaa 200460atgagataat gagaaaagtt tcattatcta aaatgactta taattgataa ggaattttga 200520tgtttctttt ttgttgaagt cttagttgat cttttttatt aatattttga atgtcctctt 200580ttttggttca aaataaccgt ttatcataat atttacataa gcaataaaat tgtgcaattg 200640ctactcacta gaaagatttt aatttcaaat cagataaata tagattgata gcatgagata 200700actacgaaaa tgtatatcat ttaaattatc agtaaaacat tttgtgaaga tgttaatttt 200760tctaggataa cgtttctgtg aattaattgt tccttaacaa aacattttta gacttaaact 200820tagagatcct aaatgctctg catttcctaa actttgagat agagaaaagt aggcctgtct 200880tttgataaaa ttgattacat ttttatcttt tgataaaaaa ttgattgtat gtattatgtt 200940tttcttatca aatttgttag tagcattcag aaacatttcc aaatgaattt tttcctactt 201000ataaagggaa ggaaaattga aaaaaatgac tcagactttt tattttttac ttttttagtt 201060
tttattttta tttttgtact tcaaaaagtg ataagttcac gtaaactaca tgccattatt 201120attcataatg ataattgcat tttctataca atcgatatca tttccatttt tattataaaa 201180cagcagactg gtttctgtca taaaacaaat ctggcttgta ttagaacaaa tacatcctca 201240tttaaagaat tttaaggttt ctttcttgca ttgagtatct taagctttat aacaacgtct 201300ggctcttaat tttagtaaat ggcatgacac actatatcca tggagagctt ctgacaaaaa 201360cttacttatc agacagaatt tcttcggtgt gttgtttact cagatggctt cagtttctct 201420ttagtaaaat aatatttcca tgttggtgct agagtacaga aaaatgttgt tactgtgcac 201480gcatcttctg cagatgatat cctgttccct ttctttgttg atatttttct ttcttaaact 201540ttgttgctca gaatatcttt aatgctagcc aaggagtcag ggatttggac gtattttcat 201600ggacttccaa agcttttttc ccccaggtga gttaactgta aggaacaata atattttatt 201660caggtatttc agcatgtata acaaattttg tgtctttaaa acctttgctt aagattaata 201720aaacctttta accaaaaaaa gatctatatg aagtgctgca attgagtttt ctcactcctg 201780ccaaaaagaa tgtattatta gtttaagtga tacttccaca tgacttcaaa tttgctatat 201840ttagttttat gagtagcttt tccaatgact atatctaaga ttagtgtttt cacaactaac 201900ttatttaggt gacagtgtcc tggccttatg gctcttttat tttttctgct gccaacactt 201960tcacattccc tctttgaatg ttcatctgag aatgggatgg gaatggaggg agaaaataat 202020aatattgaaa ttcatgaatt taatatgctg gtacctgggt cttacatttc tgtattgaat 202080aaagcatgta ttttactatt gcttgagcat gataggcaca gttctatgac tttatgtctg 202140tcagtgtttt atactgttta gcacaagaat tggataaact ttgttttata cttattaata 202200tagttttatt accttttcaa taaaatatta ttttaaattt ttaacaagtt ttcataattg 202260cctttcagtg actagtttgc aattataatt agttgtgcca aggcttaact cccagtgtta 202320ccctgaatga tcaaaagtta gaattattgt tataattaat taaatgcact aggaatatta 202380ttaaatacca gtgaagttgg gtttttttgc taaatctatg gatatttgct tttttatgtt 202440gccatgtaat ttttaagaat cagttgtgag tgggattaaa acttgcccct tccaactatg 202500aaatggcatg atgtatttat ttattaacca tttacaaaca gacattttaa aatgtttaca 202560gaaaatcttg tgagtgtaat agcaagtact atgtaaagtt tagacctgtg ttactctcaa 202620tttttatctt gagtgctctc tgtgtattca tgtaggtttg catatatata aattcttaca 202680aatatatatg atgagtatat aaaatgcgta ttttacattt attttcggtt gtaaatctaa 202740
gttttttgag tgtttaaaat tctcatttcc gttaaatttc caatatgtaa ccaaagaata 202800ttggtaaata aaatataatt acagtaaatg taaattaatt tttcttagta catatttgta 202860taagtaaaaa ttggttattc atcactatag tgagattcac aaaatattat gtgaaagtta 202920atggtgactt taacattttc cctgaccagt catgtatgga attaacaaag tagcaggtat 202980acagaattct tgaatctaca tatttttaaa cctgaggatt ctatttttaa tattctactg 203040tgttactgtg tgtatgaaca tgtatacata tctcttcatt atgaggctac tggtagggat 203100agaactattt aatacaattc tatcttgcat aatgttagtt gttaaatttt tattctattt 203160ataacattat taaatatcag ttttaaccta atgtttgcat gtgtgtctct ttatggttat 203220ctattactct atctgaatat gaaataaatt atagagttca tattactgta tttaaaaatg 203280cagttttaaa tctcttttaa aggagtaatg atgacaatga gaacacccag tgtggaccct 203340ttatttacat ttagttggga aaaagagaaa atatggaaag gttattattt ctctttatta 203400cattcacata ggaaacaatt ttatcacagc attacaattt aacagaaaat ttagggaagg 203460ttctttcatt ttagaagtga ctcttttcaa taataaattt acgttatatt tacttaaatc 203520catgtttaat ttctcctttc tactaaaaat atagtctgtt tttctgttac tagcattaat 203580gtatcactgc atagtaatgg cttaacaaat ctagcaagac gtttttattt gtacaatatt 203640agagagtata agttgttaaa atattgtaag catcaatatt tcattcataa ctaacaaata 203700gcaagatata gctatatttt taatgcatat ttttcatcat ctctgcttat ggatcagttt 203760tcagattctt catacctgtt ttctctattt ataaataatt atttcaacat gggaagaaat 203820gaatatttag actttttttt acccataaca atatacagta atgcagtaat aatgattcaa 203880aattattccc ttgtggttca catagtatag tatttttatt tttccaactt taaatattgc 203940attaaagata aaagtaaaat caaggatgat tatatttctc aaattatggt gggcagaagc 204000cagaggttga ctggtttcct gctatatttt cttgccttca actttatttt aaatagcatt 204060gttgaaaaac tgagattctt taattagtgc taatgactaa ttcttcagtt atctttagtt 204120atgtgttttt tttatattga cagtactgta gacttgctat aaaagctatt aataggaagg 204180ctttgaaagt gataatagat tgtattttgt ttatttttct ttcctattca aattttcagg 204240aatctaaaac taatactgat gactttttca aagacataaa ctcctgctgc ccacaggaag 204300caacaatgca agaacaagat atgccattct tgcgaggagg gccaggcatg tacaaggtag 204360tgaagacggg accttcaggt cacaacatca gaagctgccc taaccttaga ggtatcccaa 204420
ttggaatgtt agttctggga aacaaagtca aagcagtggg agaggtatgg attcttgtag 204480cttcatgact tctaagatac tcatttttaa tgtaatacaa ccaaagcatc tcttctaaag 204540gttgccattt ctaccacagg tttttcaatg ttaatgtatt ttatctttac ttgtattgca 204600aatacataat ttttctgtgc ttctgtctaa tgatttgcat aattatcctt gtgtaaacaa 204660tatattaaca aatgatttgt attgtatata taaatgtttg gtatttgcac atattttttc 204720attattacaa atgtactata ccatcatgga ttatcatcac ttaaaatatt ctatgtgatc 204780ggtatggaga tgtggtcata tgcaaaaggt ttcatttctt tgttcccatt cataggtctc 204840actgaagtcc tgagcaaata aaattactct ttaggctact gtggcatttg cctcagctga 204900taagcttgaa gatgtagtgt gtcatgctat ttctcagtaa gggtcatttt caagttacac 204960tatggatact ctcaacctaa ataatagcaa gtcggctgtg ttaggacatt ttagatgcag 205020aaaaaagaaa atgagccatg tgttcttggg taattcttaa aattatttag gtgttatctt 205080tgtgctttga aagttattat tttaaccaaa ttcataaata agcagtattt tacagaactt 205140tttattgact tctgtcttac ctcaggctgt ggccttaatt tcagaaacta atttcttagt 205200agaaatgtat tggaatgatt acactattca gacacgtaag atttttactg gttttcagac 205260tgagttccct gaaagaatca agccaatcaa tcatgctttt tgttatgtac caggtaacca 205320attctgaagg gacatgggtg caactggatc agaacagcat ggtagagttc tgtgagagtg 205380atgaaggaga ggcatggtcc ttagctagag acagaggcgg aaaccagtac ctccgacatg 205440aagatggcaa gttggcttaa atttgtgatt tattccaatt aagctttcaa atacagaagt 205500ttaatgccta ttgtattgct tttgttttaa agagtttttt gtgtgtttgc tttttaacaa 205560gcaaaactaa aagttaaaaa atcttgcaac tgttaaaatt ggtttgtaaa atacgtgagt 205620acttaatgca cattaaagaa gaaaattaaa agaaaattcg aaacatatag actcatcttt 205680ataaaacagg cttttaattt cattaatata aatttatata cctctttctt ctgtaccagt 205740ccaggactga acgttctatc ccttctataa taaaatgaaa cttcggtatt ccaactacca 205800atctcagtac ttctcccagg caacttcatt tgattgcatt ttgtcttagg gaaagagcat 205860tgaataaatg catagtgtct ttagggtttc ctgttgttag attttcaact ttaaatttta 205920ttttaccttt ttcaacatgg ctatcactta ataaaatata ttcctttttg tccccctctc 205980tttgatatct tggtggcaaa aatttgttct tcgaagttga gaccacatta tcacttgtgt 206040gatgctttaa ctgatcacaa cagagcaaag aatttcttgt gttcctaaag aattgtgctc 206100
accactagaa caatagtgtc atagtgaagt tcatgtttat ttcttcatac ccagttatga 206160gtcccttgtg gacaggcaca tggtttttat ctttccattc ctagtactga aacagttttg 206220acacatacta gttgctcatg aagttttgta atacaacttg aattataaaa ataatagcac 206280atcaaaataa gtatgtaagc aaacatggac ttgtctgcca aatcccaaaa tttataggat 206340gaagttaggt aaataagaga acatgcatct ttattcaaaa gataataaat ttataatact 206400tattattcca agaggcaatt tggccataaa tatggattca gaaaaggttt gaaatttgca 206460ataatgatgg agtatctttg gagatatctc cctttttccc cccacaacac acctttgagg 206520taggtgatat aatcttaaat ttataggtag gaaactcaag ctctagaatt taaactaacg 206580tgtctatggt tgtacagctg gtgacatagc agatgtaaga cttcagccag aatcttcatt 206640cgtgttttaa atgctgtact cattacacca tgctgtacac attgagcaca acttgagaac 206700ctacctcctc tccttgtttt tcccagatag acttttttta gtctaagatg atatggtcat 206760aaaataatgt cctgaaacag tagaactgtt catttgaaaa agataagggt tccgcttgat 206820gtaaatcaga catttactaa atgacttttt cacttactat gtgcttatta tatattgctg 206880tatatttact tcatattcat aaatttttaa acatttttct ttatatagga atattcagat 206940ataaatgaaa atacctaagg ggagatcagc tttatttaag ataagttaaa tatgagaaca 207000gcttaggtga cttctaaagg acttttctag cttaatcctt aataattttt taactggcag 207060tacaaaaaat atgtaagagt ggattctgaa gcaggactgc cagggtactg atcccagctg 207120tgcaactttt tactcatgtg accttgacca agttaaccta atatctctgt gcctcagttt 207180tcccatttat aaaatgacct tataggtcat aaccacataa atttgatatg aagactaaat 207240gagcaaataa cagataaagc taatggatgg taagttaggt cttattgtta aaatgcaaaa 207300tgccttccat gctaaggatc aagttttaac ctgggaaatt ttattcttat aaacttagaa 207360gatagaagct tctttcaaga taaaatgcct cttaaattag agtatcattt aagaaaagtt 207420taaatttttc tctgtaatta gttaatcatc ttttactctt gatgtataga tacagacata 207480aatatggata tttggtgatc atgtgaatct tactatttta atataaaacc tggagtgata 207540aggaaagaaa tgatatgaag ttagtgcctt taaatcacta agattagttg atgtgctgcc 207600caaatagtct gttcattggt gataatattt aatatggaag tacaaagatt tggcactatg 207660gtaatttata tgaagtcatg tgagtaaaac aaaagctttc ctctttgtaa aaattctcta 207720ttggtctttg aaatctgtag taggcaaagt tactgctgtg gtaaaaacag acttcactct 207780
gggtgctaga ccattcttag tctagagagg aagcagtgct atcttttccg ttcagtttac 207840tgttcatggg tgtttttctg aagaaacatt ctactgctga aatattgcat gctgaagagt 207900attgcctaag ggaagtgcta agattggttt tttaaatcaa gtctacaaat gctggtaaag 207960ggatagatta tacagtacca ctggtttagc agaattgcaa tttacaggta ggaaggaaac 208020actctaccac accctgtctg tagttccctg ttctgtttaa aggactattg actttttttt 208080tttttttttt ttgagatgga gtctcgctct gtcacccagg ctggggtgca gtggcgcgat 208140ctcggctcac tgcaagctcc gcctcccagg ttcatgccat tctcctgcct cagcctctct 208200gagtagctgg gactacaggc gccggccacc acgcccggct aattttttgt atttttagta 208260cagaaggggt ttcaccgtgg tctcgatctc ctgacctcgt gatccgcctg ccttggcctc 208320ccaaagtgct gggattacaa gcgtaagcca ccgcgaccgg ccaaggacta ttgactttta 208380aaaaagatat ttaagatact taaagatatt ttaaaaataa gatatttaaa aatatacttg 208440agatatttta tttttcataa ggttatttta atggaataaa taccttttcc ccccaatttt 208500acttgacaga ctttattttg ccttttttgg ctggcattaa tatggatatt taactaaggt 208560attgttttat atcagacaga gtcttggagc cagttgattg ttcatagggg attaatttag 208620tttgcatatt ttataggtca catcttgcca tttgtaaatg gataaaacat ttgccatttt 208680ctgcttgaaa taagaattct gatacattta tagactctga ttatgtaaca tggtttatta 208740ttatccttct atttgggaaa aatatcttta tgtgataaaa taagttaaaa gttaagtttt 208800ccataatttt cacattcata acttagaaca gttaacaaat gaaaattcct tttggtacta 208860gatacttagt ttttttctct attctaatac taatatgcaa aatactttta tttctggttt 208920ataggttatc cattttgttt tgcaaaatac tcttgtataa atttgttgca aatacaaaga 208980gttcctggtc cagaaatatt agtatgcatt cagtaatgta tcctgccaca ataaaagtaa 209040attggcttca taagagccta tctcatttct aaatttcact gtgtatttga taaataaaaa 209100caaagatttg tcaggtgctt tgttaatgtt aagatgacct gactggtatt taaattgatc 209160aaatctagat gtgagcttat tttaaaatct aaccatcata aaatcttttg caattttctc 209220tctttgttgt atgaataggg aatagcacgt aagaaactaa tgttacagct atggctttta 209280ttgtagaaca agctcttctg gatcagaatt ctcaaactcc tcctccaagc cctttctcag 209340tgcaagcttt taataaaggg gcaagttgca gtgcccaagg atttgattat ggactcggaa 209400atagcaaagg taggtattta aaagtaagtc ttttacttac ctaacttcaa tattttttat 209460
taccttctct tttatacact ttttatttgt gttcacccag aaatctttat taaattaaaa 209520tttgttcatg gaaagctaat aaaaaatgtt tacaaaggtg ttcttttcat agtattgttt 209580tttgctgttg ttttttgttt ttaaccagtt ttggcttaag tttatgagag tgcttgctta 209640ttttttttct atttaaaaaa cactcatttt gatttcatat cctaaagatt ctatttcact 209700aaatctagta aaatttgatt tttatcatcc acttattagg atggtaatgt taatgagaaa 209760cagagaactt gctcaaatga tccatttaaa atcaaactat tacttctaaa taatattttt 209820aagtaatatt acacatgagc tttataaaat ttatgtggtt ttgaatttct aacccagtaa 209880tcttagttaa tttagaatta tgtgaaatga tctgtttggt tttaatatct ataataaaac 209940cagaaagttg taaaaagaag aaaaccctaa gtcatatgaa tataatatat atacagctgt 210000tttggctgca ttaatggaag agaccagcat catggactgt ccaaacaata gttaattttg 210060aaacaattta tgctggaggt tcatcagaaa ttgcattttt atgtgaattc acacactctt 210120gttctttcct atgtacctga tctagactcc ttcaaaatca gccctattgt ccacaggtaa 210180catacagctg ggtcatgagt aaaagggagc agatagctta cgtggaaata attttacttt 210240aattttattt aaattaattc acaggacttg attccaaggg acaaatacag tgtcattata 210300cctgacttga agaacaagtg aaaaaataaa acagtgaaca aaatgtataa ggaatattct 210360gtttaaaata aaactacttc tattgaatag cttcattact ttccacaaag tatttttatg 210420aaggaaacct agccacaaga ttttttttta ctcccttgga ctagcctatc tcataggatt 210480gggtatcatc tgctcgctta cttcgggtac ctgatttcca ttaacttaag ctagatcttt 210540gtacttacat agtgtaaatg cttggtggtg ttttctttat gtccctttct ctttaccttt 210600gtcaactctt aaaatctcag catttgctta ccgctactat atctctatat agtaatgttt 210660taaagccttg tttaacacaa attgtatgct ttcagtcttt aaactgtctg tcattgtact 210720aatagctatc acagtaaatc agtcattata atttattaaa atatcattaa aataaatata 210780aatgtttgag acacaaacat tgccgagaaa atttgttatt atgtacaaat aataaagtat 210840tactttttaa acatgagatt ttgtgaatat cgttcaaaaa tagcatgcac catgccaggc 210900atagtggctc atgcctatag tcccagctac ctgggaagct gaggcaacag gatcacttga 210960ggccaggagt ttaaggatgt agtctgctat agtcgtgcct gtgaatagcc acacgctcca 211020tcctgggcaa cagagcagga tcctgtttcc ttaaaaaaat attaataata agcctactct 211080tgttaacaag cttttaaaat atttagaagg agcaaagaaa taataagaaa cttaaacttg 211140
atgaaatata tctaacttta ttatctgtaa cagtagttcc cattttaact cttaatactg 211200tccaagatcg aagagctgtt ttttgtaagt ttttttttct taaaacagta ttttagaaaa 211260ttcttcatga tctctaaatt ttttcctata atctacagca tttatttatt atttcaaaaa 211320aagatatatg gctcgtgtac acagttcttt ccacataaat ctatcccgtg taggtacttc 211380agttgaaatt ttcacatatg aaactttatt atatttcaga aatatgacta gaaagatact 211440attttccagt ttctgcatca cctagtctgg atgtagttcc ctctccccac ctccagcatc 211500atttaaacta tttaatgtat actcacaaat aaggagttat tatccatcac ataatttcat 211560tatcttaaaa aaaatttttg taaaaccttt tggtacaagg aaaaatgtta gaatcgatat 211620tttagatact attcaaatta ggtagctcat aatccttttt gtcatatagg acttacagat 211680ttttaaatta ttcatctaag caaaatattt catgctttta aagtagaatt tctttggcag 211740taacaactta aaaaagaaag tattaattta taactataat gtaaacacat tgctatgtta 211800ataacatact ttgccccatt ctgctttttg aagaaaaact gttaattgct tattttctgc 211860atgaaatata ctgccgatga ggcagggtgg tattagtcat tgttgggaac tatggaaaag 211920gcatcattta cttaaatctt catttatata tgttcaatat tgaagcaatt tatcactgat 211980tcttcataga gtcttcgttc tcccatacat aatgagagat gttatatatg aatttaaatg 212040gatttgtttt tataggtggg cttctatata agatttggtg gggcgcttcc attaaaaaaa 212100aaagcttgca aaattcggtc cctaatctga ggatgattaa cagtcaccaa ttttcactca 212160tttctgaatt ggcatggttt tatttagtgt tcaactctat ctgaatgtca taaagcacca 212220tttactttgc cattttggat gttaatgaat aacgagttgt agttttgact gttttaccca 212280ctgcaggtga ccaactgagt gccatattga attccattca gtcacgaccc aatctcccag 212340ctccttccat ctttgatcaa gctgcaaaac ctccctcttc cctagtacac agcccatttg 212400tgttcggaca gcccctttcc ttccagcagc ctcagcttca gagtaagtct gtcagcctta 212460cctgcctcat caagcatttc tgaagctttg ttctgagttg cttatcagtt actaattagt 212520tatcaactca cctgactggt tttttttaat taggatgtta tcccctccca catttttgaa 212580atatctaagc tattctgagc taagacattc tgcagctaag acatttaaag ttgatttaaa 212640gttcatatgc atgactatgg taagcatcac atacagggac aactttcagt ggatttttct 212700tacttcaaga tgtcccaaaa ggataaagcc atttcactct tatcagtggg tagaagagag 212760actgtacttg tttggacagg tttgtttgtt ttttctaagt gaatttggta tctatgaaat 212820
gtatgacaat ttagttttcc cggttttctc aaattgttgt ttcctgtctt tatttttatt 212880tttttgttgt tgttcctaat atgttaaatt gatcatttga ttccagttct gtcttttagg 212940attgctctag tccttcattc atgatagtaa ttcacattgg tttggaaaag atacccaaaa 213000aaattatact taactgagtg ttcttttttc ttaataacct cctgaacctt ccacctaaac 213060ttttcaaagc tctgtccagt atgacaacag tgtgttcttc ttggcattca gttctcatgt 213120tggctataca atatataaac acattaattg aatgagctag tttatgaaag tttcagcttg 213180tataccattt ttcggattca aatgcacaac ctctttgctt tggtaaagta cattcacact 213240taggtctaag ttatttgttt acaaaaatac tttaataact gttattaggg tcatgtaaaa 213300gaatgctagc tggtttagtt tttattaaaa actaggttag tatgtagcta gcaaatgata 213360ttattcatta ctttatccac cttaggtaaa gggtacacaa gagtataaac ttttctgggt 213420ttccactatc cattaggaaa gtttaattga tgaaattaat tagtttggaa atataagtac 213480tatcaatttc tagcttatca tgttcgttca tgtataatat taatttgaat gtctattaag 213540agaagaattt caaagaaaca aaatagtgtc acatgtgcga aatagttttt tccttctatg 213600ataaatatta ttttagaaga atgaaacaga agtgaggagt tagtttatta attttgtgtt 213660gcatcagact ttttacattt agaagtacta cttaggtttc aagaagtttt aaaggtaagg 213720acaaagggaa aaacaaagga aaaatatcag gatcagtcag gtagaaaatt tatactagaa 213780aatttaaatt cattcatatt caaattcagt tttaaatgaa aaattgcctg tgtcatagca 213840gtagcagaca aaagaactaa ggagaaagga gaaccttttt gttctagcta aagaatcatt 213900gcaagtcttt acaaaaacaa gcataattac cattttaatt ttaccaaatt taattaataa 213960aatatttcaa aacaataaac agtaataaaa gttgatttgt ctcttccagg gaataacagt 214020ttctaggtga cattgtgtca atataatttt atgcatatgt gttatcagta ccaccacttt 214080atttaccact atcttgaagc aaatttgaaa tcatggctta gctatgtttc aattagcaat 214140tttctgaatc attacttgcc tatagtccag caatggaaat tacttaggtc ttgttttaaa 214200cttcttattc gcaacaggac gtctctaaat tttgacttcc aaattccaga aaatggaagt 214260cttaatcttt agcatcacaa aaaaaagtat ggaatctgaa aatagtttta attttttaaa 214320ataattcaga attgtatttt ttataccgtg ttgggaaagg taaatgtggc aaagtgccaa 214380catttaagac tccccaagca aggtattctt ccctgaacct cactaccatg tatcccacta 214440gtatatcacc tatgctttag aagatggctt attcttagtg aacttcccta tgctatttag 214500
taaaatgaga tttatttttg ttttatacct ttgtgatact aaataaatca ttatgtagaa 214560gaccatattt actcaacttt gtttatactg atagtcgcca ctatgtagta aacactgtac 214620ttaagcactt tgatacagcc tcatttgaga tactgtctct tatgtctgtt ttacagagaa 214680ggaaacatag gcttagagag gataaatgat ttgccccaga aacatacata gtaaaatggt 214740agagccagaa cttgaggtca attctgttga ctacaaaggc tattttaaat attcagcctt 214800acctcggtaa ataatgcaaa atctacttta atatatatat atttttaatt tatatttaaa 214860ctaggtctgg catctagagt attagctttg ttcagattga ttatttttct ttttatagtt 214920tgactttttt taatgtaaca gtagtttgaa tggaaaaaaa gatttcctga atgtcataac 214980tcaagatcct ggttaactag aaaaattcta caaagacaca catcaaccgg tgcatggttt 215040aacttcatct agcatttcta tgagcattat tatctgtaat tctttggttt tttttttccg 215100aatcatagtt tttacaccta tctttggaaa tttgaaaata atctggcaca ttaagttaat 215160ttttgatacc ttttctttag aatcaggcaa cattttattc cataaaatag aatatttggt 215220aatttttaaa ttagcaaaat tatttgattt tatatacgta gaaattattt caagtgttat 215280tctagtgaag tagaatgtta tagaagtaaa agttaacaaa tttcccttgt cttatgtgtg 215340tgcacacaga gacatgaaaa agaaaagtat gtatatagaa ttttctttac tgttctacaa 215400tttacttttt aatttaaaaa ctaattatat agtttttaga attggtaagc aatttggcaa 215460aaaaatttta gtgaaccaaa gtggagaatg atatctgtat ctgctttctt taagaatatt 215520acattcctta ttttcattat caccatttct cttgaaagct tcattttatg tcctgtacct 215580tctgacagtg tcttacacat cacaggcatt caaggagtgt ttattgactg taggtattta 215640agattaccat ttcatcaatt taagatcttg tgttttctat tatgatattc cattgtttac 215700tcagagaaca tcttcaaaga agagaattta tttctttctc ttagtaatta aagccaacta 215760tactgtgaca atatgatgta tgataattgt gggtttatac tgattgaatt ctaagcaaga 215820ttatccaact gaaaaactat attgcaaatg gcttcattta acatagcatt ttatcacttt 215880ctacctactg ttccagaggc tgaatagttc atttttcaac ttttatttat ttgtttgtgg 215940tagaaaaatg gcaaataaaa ctgaagacaa tttgcttttc tcccagaaac tttttaatac 216000tttttttatt ttataatgtt ttatttttaa ttttgatggg tacatagtag acatcttcca 216060gcaatttcaa acatgaaata tatgatatat gtcaaaagac agtactttca agcacagata 216120tcatagctct aggttacctt gcaacaagag tttttctatt atactcataa gtcacattct 216180
tatgtgggac aaaaaaaatt aaatgtgacc ccaaaattca tttggatatc tgcatgacta 216240tttatttaca tttgagaata tgaacacact aaagtactat gtttacttag cttgtagaaa 216300catagtcatg gaaagacttt cagctcagat ttcccttcca gctacttact agctctctaa 216360gttatctgac aggtctgaat ttttgttacc tcattataaa atgggattcc caataaatgt 216420tatcttttga tgttattatt gccttttgat gcaaatactt aatacctctc atgctttgag 216480gaaaatatag aaaaagtcct tctctaaaag agagcaatct attcttggtg tggaatgagt 216540tgcaggaagg aatttagaaa gggacaaatg agctttccta gttccttttg ggtctatttt 216600tgcaatgtaa ttcagttacc tattttaaga ttcttaatgt atttagactg cattcagatg 216660ttagtgtgaa tgttagcata aaagccaggt ttgtttgtaa ggcttagact gagtcatttc 216720taccccggat tataatctca gcactgtatt tgtcagtttg ttgtttgcct gctttgaagg 216780tatgtggatg aagcacttga tttaggaatg aggacctgtg ggatttaatc cctgttttag 216840aagattagag tcagcattgg ttcacatatc aaagtttaat aaaacttttc cacttgactt 216900tataacatct gtcacaacaa ccaacatatt tattttagtt ttggggctaa catctccagc 216960atccctacaa atacaaatca ttttaggcat gggcttgaat ttatcttcac tgagctgagc 217020catcttctca gtgacctaaa agctttgacc caggtatttt gttttgctgc ttcatcagtg 217080atttattcat ccacccttta cttcatccta ggaattcaaa ccacaaagaa tgctgatgtt 217140gcttacaaga cattttgtcc cagtgcattt gtttgctttc tcctggcttg atttgacctt 217200ttatctctcc ctcttccccc acctctttgt ttgctactgt gtttatttca cccttcatta 217260ccatctttct ttagatgact tattcattct tccatttatt cacctgcttt tgttaagtat 217320ctactctgtg ctaggttatg gtgatgagca aggttaaaaa aaaaacaaaa aaccatgatc 217380actgatcttg aaggcttcta gaatatttta agagatacaa cctagttaaa gaaaaaataa 217440tggcatgttt taagtgcaac aagagcagta gtcacaggat gcaatggaaa cataggaaag 217500cttctggctc agctaggaga ggttagagaa acattcaaaa aagaggcagg tcctactcag 217560agtgttgaag gacttgtgag aatttatcag gcaaggagga gtggagatga ggaaggaggt 217620taatggagca ttccaactat aaggataaca tgagttacat tacagagatc caatgaatca 217680caaagtattt tattgagtcc acctacgtgt caggcagtgt actaagcact gttcatgcag 217740agggccagtt tggagtaatg aaactccaaa gtttgattag aataggctta ggagaaaacg 217800gggaaggaga aattggagac aatgagtata gagaagtgtg tcaagatttg atctaaagag 217860
aagatactaa gagagaagat acaagatctg gagaggtttt tgttctattt tgtttgttaa 217920tggaaaaaaa agtatatcaa ggttgttttc taatgagagt aattcagttg aaagaaatat 217980taataatgca ggagggtgag gagaattact ggcacaatat tcttttatag aagaggggat 218040gcagaccagg cgtagtggct cacacctata ttcccagcat ttggcaaggc caaggtagaa 218100ggatggcttg aggccaggag tttgagacca gccaagacaa catagcaagt ccttgtttct 218160ataaaaatta agaaaaattt ttttaaagat cagctgggca tagtgatgca cacctgtaat 218220cctagctact tgagaggctg aagcaggcaa atcacttaag cccaggagtt cgaggctgca 218280gtcagccata attgcatgcc accccactct agcctgagca acagagtgag accttgtctc 218340taatatgtat atatacagat ataatatata atatataaaa tacattctat ataaaatata 218400tattatatat tatataaata aaatattata tataaaatat atattatata aataaaacat 218460tatatataaa atatatattt tatatatata taatataaag aagaggggat acagtccagt 218520gcaaagtgga aaggttggct aagtgggatc ataaatagtt atccatcata acaagcaaga 218580aagaagtatg tatattattg ggagctcttt tttctaagta aaataggaag cacttatcag 218640ctcggagtta ccatggagag actgcattga tagtttgagg aaagagaaga tatgagattt 218700taaaagctaa catatgtcaa atatttatca catgccaggc accattcatg ggaatcaact 218760tttatccctg tccaagaaca tggttcactt gagaaacttc atgcatgtgc agctttaagt 218820gacctagtgt cagtgtctta gactagagag gttggggctg gatcatgaag ggtcttgtaa 218880gccatactta gtttagactt tatgtgcagt gaataaatgg agggagcaca tggaggaaat 218940tgttctgcag aattttaaat aaaaagagtg atatgatcag ttttacattt gggacagata 219000actgaaaata ttatgaaaaa ggtcctggag aaagggagaa tgttggaggc aaagaatcca 219060gagaaaagct gagtagggtt taagctaagg ttaattgcag ggagaatgta gaagaaatat 219120ttaggcagta aaatgcttag gaccagtctg gacaacaaag cgagaccctg tctctactta 219180aaagaaaaaa aaaaaaaatt agccaagcat tgagggctgc acctatattc ctggctactt 219240gggaggctga agcaggagga ttccttgagc ccaggagttt gaggctgcag tgagcagcct 219300gggcgaaaag agcaagaccc tgcctcttaa aaaaaaaaaa aaaaaggaag tgacatgctg 219360attaaagtta ggagccagtc tcaaatgaca tctactttta tgctctgttt tgaaaggtaa 219420tatcttatct agtaccaaga ataaaacaca aaaccacctt ttaaggaaaa atgagttcgg 219480ttggatgact tagctccagt tggctataat ataacttgaa attgagaaat atatttagca 219540
ttctcataat taaagatggc attgttgact gacaagttga aaatagaaaa ttattcatat 219600attacatttt attaaaaaat attattaaag cattctcatg attttgcact caatttagga 219660aggattaggt ctgctatatc tcccactttg ttattctctc aaacttctgt acctgtagta 219720tatctcatta cttaagctga acaaaacgtt cattttttgt aatcagactt ctcatcctta 219780tcatcagatt gtctttcctc ctttggttta tttaagaagt gtgcatcagc agcagccgtt 219840atgtcttatg atggcataat ttcaagtgtt ctaggggcct aagaagttat ccagctctca 219900ttttgagatg agagaattct tgctgacttg cccaaagtca tagctggcaa atctaggact 219960tgaacatgag agtctgtatt gggggaaccc gcccccaata tttcaacgta ggttctttct 220020attttcccta agcattggcc agtctgagaa aaaaagagaa agagtacaaa gaggaatttt 220080acagctgggc ctctggcggt gacatcacat attggtagga ccgtgatgtc ctctgagcca 220140caaaaccagc aggtttttat taagcaggtt tttatcaaaa agggagggga tgcaagaaca 220200gggagtaggt cacaaagatc acatgcctta aagggcaaaa agatcacaag gcaaagggca 220260aagcaaagat cacaaggcag agggcaaaat taaaattact gatgagggtc tatgttcagc 220320tgtgcacgta ttgtcttgat aaacatctta acagaaaaca gggttcaaga gcagagaacc 220380gatctgacct caatttcacc agggtggggt tttttccccg ccttctgagc ctgagggtac 220440tgcaggagac cagggcgtat ttcagtcctt atctcaaccg aataagacag acactcccag 220500agcagccgtt tatagacctc cccccaggaa tgcaattctt ttcttagggt cttaatattt 220560aatattcctt gctaggagaa gaatttagtg atatctctcc tacttgcaca tctgtttata 220620ggctctctgt aagaagaaaa atgtggctct attctgccta accccgcagg cagtcagacc 220680ttatggttgt cttcccttgt tccttgaaaa tcgctgttgt tctgttcatt ttcaaggtgc 220740actgatttca tgttgttcaa acacacatgt tttacaatca atttgtacaa taatggtcct 220800gaggtgacgt acattctcag cttacaaaga taacaggatt aagagattaa agtaaagaca 220860ggcataagaa actgtaagag tattatttgg gaactgataa atgtccatga aatcttcaca 220920atttatgttc agagattgca gtaaaaacag gtgtaagaaa ttataaaagt attaatttgg 220980ggaactgata aatgtccatg aaatcttcac aatttatgtt cctctgccac ggttccagcc 221040agtccctcca ttcaggatct ctgactttcc gcaacaagtc tgctaactca ttccagtggt 221100tttttccaac tgcatctcag ttatcttaca tagactgcaa gaagtgagaa agacaagagg 221160ttatctagtc cagccttgct attttatagt ttaaatccct caaccacatc cctgatgaac 221220
ttttgccagg ccggtaatta acaatatcac aaggctgttc tgattgtctg tatttctcag 221280tgtttgttag agcagggatg tccaaccccc aggccacaga ccaatactgg tccaaggcct 221340gttaggaacc cagctgtaca gcaggaggag agcattactg tctcagctct acttcctgtc 221400agatcagctg cggcattaga ttctcataag agtgcaaacc ctagtatgaa ctgtgcatgc 221460aagggatcta ggttgagagc tcctcatgag aatctaatgc ctgatgatct gaggtggagc 221520agttccatct tgagactatt tccccaaccc cccatcatat ggaaaaattg tctcccaaga 221580aaccagtccc tggtcccaga aagttagggg accattgtgt tagagaacta aggaaaccgt 221640cctctacctg ccacataaga ataaaggaaa caatggaaca gtttccctac tttccctaat 221700taacacgtat tcccattttg aggcagtgag ttgctggtac ctgctttctc cttctttctc 221760caaatagtac tttaaaagta tcttatcctg gctgggcaca gtggctcatg cctgtaatcc 221820cagcactttg ggaggatgag gcgggtgaat cacgaggtcg accatcctgg ctaacacagt 221880gaaaccccgt ctctactaaa aatacaaaaa attagctggg tgtggtggca ggcacctgta 221940gtcccagcta ctcaggaagc tgaggcggga gaatggtgta aacctgggag gcagagcttg 222000cagtgagctg agatcgtgcc tctgcacttt agcctgggca acagagcaag actccatctc 222060aaaaaaaaaa aaaaaataaa gtatcttatc ctaaaagcac ttctgttttt ggtttaggtt 222120ttagctgtct ttgtgctgcg taagcgttgt ctctttctca ggatgtcact tctggaggca 222180ggaaagggtt atggttaatg ctaatcactt tatcaaaatg tctgatttct ctgatgtata 222240attaatattt ttcccttgca actaataagc aacttgtggg agtaagattt ttacttttaa 222300aagcatatcc aaccttcttg tgtctcaagt taatgctcgg gaagcaatac ctgtctctta 222360tattttgaag ttattctttc taaggcctaa gatatatacc catcaaatta tgctcagaat 222420ttttgtctca gccatcacaa accctgaaat gatacgcttt tcctaagtgt tattttcctt 222480aaccatattt actagagatt tggaatatat tcaataatat gctatgagta tgttttcttc 222540aagttcaatt atgtgtagat gtcatttaga gaaacagtat attttggggg gaatccactt 222600tatgtgtccc atcttctttc cacattcaga tcatgtggat taatgtcata gtctagtaaa 222660aagcttgttt ctattttatc cttaagttta aaatgttgtt ctttcattct attagcatga 222720ttcttttagt atactaacat ttatgtaaat aagtcttaca taaatacagt tatataaaca 222780aatctttagt ctaattcttt tagtatacca acatttatgt aaataaatct tagtttctgt 222840tccacgtcct aagctgcttt accattcaca taattccata ttcctcctaa gtttactaca 222900
aagaagaata ttagatgtat tgattatgca gtgatactgc atctaaagct gtcagtcaag 222960aatggctgcc atagctaaga gtatatacaa atcatcactg ttactttatt ttattttttt 223020aattttatgt atttatttat ttttttgaga caatttcact ctgtcgccca cactggagta 223080cagtggtgca atctaggctc actgcaacct ccctctccca ggctctagtg atcctcccac 223140ctctgcctcc tgagtagtga ggactggagg cgagtgtccc cacacctgcc taatttttgt 223200atttttttgt ggaaatgggg tttcaccatg ttgcctaagc tggtctcaaa ctcctgggct 223260caagcaattt gattgccttg gcctaccaaa gtgctaggat aacggacttg agccactgtg 223320cctagtcctg tcactttctg aaaaatccta tgaagcatta atagaagtaa aatcacactt 223380aattatgcct gtttttaaat ctgcatattg tttgattaat cacatctttt ggccaagttg 223440aaggcataat ttatatattg tctataagaa tcaataaact agtttctaaa tatgttttaa 223500ataaataatt taaaaattaa tttctcaaat atcctcaact ataaagttag acctatttac 223560tgtttcttgt aggagcctat tgctgtcaaa aatatctgaa gttctattca gagaaaaata 223620gaaatgcaca atgactggga cttaagctga ggaaagtcag caacactgtt cccatcattc 223680taccataaat gtggcaaaat cttattgttt gtgacagatc tatagaatta gattttctta 223740tatctaaaga aatatatata aatacataaa cgtaggtatg tatagcatga acagaataca 223800gtgctatttg accattgtca caagacttat ttataaaacc tcctcaagct cttgccaata 223860tcttagttaa gattgcatct aaggtgggag gaggatgaag attgaagaac tgtcaggtaa 223920tgcttgatta cctgggtgac aaaattatgt ttacaccaaa cccccatgac acacagttta 223980cccatgtaac aaacctacac atgtccccct tgaacctaaa ataaaagttg gaaagaaaaa 224040aattggaaag aaaaaaaagg aacttaaaga gaagaaaaag attgtatcta gtagttttac 224100taagactata aaatttctga cggctccact acctcctcaa gattgagaac taaacaacat 224160gagaagcaca attacatgcc aaagattctt tttgctgtct tcatttgtag catcccagtg 224220gaatgtcttt gaaaaaaatt ttttttatct gtattatctc acctgctctg agtattggtg 224280acagtgatat atgcattgtg attagacgcc tagcctcctg aatataatgt atttccagta 224340tattgtcctt ctgaatagcc tgtttacaat tctttgaaca ttgcctgcca ataatttagc 224400aaattgttat tgaatgtcac agcttgatta tagaacatag taagaaatca acaaattgaa 224460caccaaactg aatttaatgc taaaaattat ccttttaaaa aataatgtat tactaatttt 224520tactcatgaa atttaacatt tccaatcatt gcttagttgt tgactcaaca ctcaaaaata 224580
atatatgcta cttttaaaat cccaaaacct ccagatttgt gtactgccac caggtgttga 224640caggtgcctt gattcttcta gtttgtggtt ccagagataa ggtcagggtc ctgatacagc 224700aaaaccacca tttgtctttg ttaaactgaa atgagaatgc gttccttatt ggaaagaaaa 224760gttacacggt tttattatta tcatcagtac ttagagaaga cttaaaaagt acaacttaaa 224820atgaactcaa agtttctgtg actaggaaat gatatgactc agggaattta atacttggga 224880tccaaaacaa agaaaacaaa atgacccata aagaaccatg ttgtttatga gggcaaacta 224940tagtaagcaa acaatattaa cttcaaatga gcactggaga aaaatcctga ttctttaaaa 225000ttgtttttaa tattattctc ttaaacttag tgatacccaa aaaagaatca aaattttgtg 225060gagaggaata taataactgg ttgtcttatc tgtattaaca ggattttgta aaagcattat 225120tatttctgtc aattgatttt taaaacatct atcaactttt tttcttttgt gtatagatat 225180caatgtatct catagattgc ttcccttttt taaattaaaa agtataaatc tttataaaaa 225240ttaactataa ctttatgaaa gtatgtttaa acttgagcag attttgtaaa aatttaatgt 225300gtagaacatg tgttgttttc agcaacagct taatttcttt agtcacttaa ccttttttag 225360acttttgttt ttaaaaatat ttcttttttt cactctgaat catacctagg aatcaagtct 225420ctctagcctt tgtaatatat tgaaaagact tgaaggccat tttgccttca aacttttttc 225480caggagtttg tccttaaacc tctaacatct tataagtttc ctcatagttt ttcacatttc 225540tgttttatgt gtgcttccaa ttaagatggt attcttctga tttcctattc ctgtgtcttc 225600ctgctatgtg gagtcttcac agatttttag cagccaggcc aggagattgt attccttcct 225660atattggcag gttggattcc ccctgaatta actcttccat tcagaggctg ccaccgttct 225720agttcgtgtc ttgaagaatt gtatcaccct cctaactgat attttaacaa tagctgcaaa 225780ctccttatta tatgacactt aaatagcata agagctcctt ttgcagcacc tgacatcttt 225840tgaggtttat agatttgttc aaaatgaagg atgtacaaaa atattaaaaa tttaactttt 225900taaaacttag attaagatgg ctttatgcca tggtatgttg ctatctactc ctcccattgt 225960ctcttgagcc cactgcattc cagctttagt ccctatcctc ggaaactgct gtagtcagtc 226020tgtgactttt gtgttgccaa attcagttgt cggttctaag gtgtcagttt tacttaatct 226080gtcagcagta tttgacagag ttggtctttc ttcctagaaa cacattcatt tgcctgccgg 226140atatttcctg ccttactggc tgctgaattc tagtctcctt tctacatcct catctatttg 226200gtcatctaga aatgtcctag ggctttggcc tcttctaggc gtcactccca aagtggtatt 226260
ctgcagtcat ggagaatgac acatctgtag gcatatgact cccaagtttc tcagcacctg 226320atgtgatgta tacttatttg ttcattccct ctctcctttc tcctaaatat aaactctgtg 226380agaataggga ctttgttcct gttcaccact gtgtctgcaa tgcatagaac agtatctaac 226440acgtaatata tactcatact ttacattgaa tgatttctta atttatgggt cattcatcat 226500taatcttgat cataataatg aatcagccga aaacagcact ttttttaaga aaagtgtgtg 226560ttatgtctag tttatttcaa taatactaag acaactatct ccttactacc tgaatttatt 226620tcagcacaac cgattaattt taattgctat gagttaaaaa ctttaaatac agtgagtttt 226680tttaaattca gatagagatg ttttttagct tactgctttt aaaagactgc aatttaaagg 226740ttctgttaat tcttcaaaaa aagataaatt aagtgcttca ttttcctgca tagtaaatca 226800ttgacgttcc taacattttt tcaataactt ataaattttc tttgctttgt ttgtgttgtg 226860acacgttgtg taaatgagat ttgatatcag aagtaggatc tacaaaacaa acactaaata 226920ctggggttag tactaacgtt ttcactgaag atggacccag aaaagtagcc aaacacagtg 226980tcaccccgag tccattctga gatacatact ggaaaattat agtaatcggt tcattcagga 227040aaccaagtga caataggtta agccatgagt aatataagta atataagtaa catttcttta 227100tcttccacta ttaaaaaaaa acttgtaaat ctttatttaa atgaacacca aattaaagaa 227160aactgagcca aagaaatgta agtctaaaat gaaatgctca attttgattt atcagagata 227220taagccttac taagttttaa gttaacaaat ttggacctta acacatatta atctaaatta 227280attaattcga cctttgtttt cagttttaga gaaatgtaaa cttgttcaaa aatatagcag 227340aatataaact atttaaaata acagtttata cttgcatacc atttgaaatg ctttgttttt 227400gcttgtgttc tctgctaaat tttttttcca ttgaatctgt gctgcttctt tgccaatgtt 227460ttccactgta gaatctccat ctcgcaacct tgcttctcgt gagcgcattt acaaaaatta 227520tggtgtagct gggcctgcct ctgctctctc atctctgtct cacaaactga agggtgggta 227580tacatgcatt catggatggg ccattcttaa tgaacataag gagtgtcatt aaagatgttt 227640ccgttcgtct cagtcatgtt catgccattc cattttttta attaaagttt atcattttat 227700ttacatttat atatgtttga gtaatttatg atgctcctat tcagtattat aatatagatt 227760atcccagtag ctatgcatgt ttattacatc tataggttaa acattactct tacttaattt 227820taaaacttta tgagatcatt aaaattaaaa taggtataag gaaagaatta cctaattggg 227880ggaatttttg aaatcatttt tatcagaaaa agatgatata tgtgcaaggc ataaagcaca 227940
ttttttagtc ttaaacttat tgttatataa tataaaaatc attttactat tttagtcttt 228000tatgagaaac actgataatg aacttaaagt ttttaagttt aaattagtta ttaggactca 228060tgtgtctatt tatatttata tgtaaaccta cctgtcctat ctggcataaa tttttctaat 228120attgttttcc tagtgactaa gttttaatat tttaaactta gaaatgttta aactattgac 228180aattgacaaa atttcagaca atttaaaaat atgtttttgg ttttttgaaa cactacagat 228240gcctacttaa atattttgtc ttaaagtatt atttaattaa atttaaatct tgtaaatctt 228300ttaaaatctt gtatttaaat aaaattttat caaaaagaac ttttacagca atgcctaaca 228360tattgtataa gtttttcaac taattactga ttttcttttt ttcttagaaa tagataaaac 228420agctatatgt ttctcattat acaattgaca gtataaacat ataaatagga aatataaaaa 228480tatactaaat agtattgtac aacctgtgct atatagtata tagtattgtg tgtaatagca 228540tatagtatca tgtaacctga tatatgctat atatcatgcc atgtgatata tgcatatagt 228600attatgatat agtatgatcc tgataattta agatttgggc atgaaaaaat gtatgggtca 228660tttagtgaat gaatgcttac catttaacat gtttagtaac atttaacttg cttgccctaa 228720gtttctatcc tgtcattatt atttcttcag gtttccttta aagttagaaa aatgtttgca 228780tattatcatt taaatgtaaa agtacagtca ttaaataaat taggagatga atgtgaaagt 228840attacgtata gtctgaagat ttttagcaaa ctgtttatat ttgttggtat tttgagcagc 228900cacaacatac aatttttagt aatatttaat aacatgctgc aaaacatttg aaacacaaat 228960gaagcagata aacataaatt agcacagggg cttaccccac ttaacaagca tagttttttt 229020taatatataa aatacgtttt ggaaaaataa atttttcaaa aagatttagt tatcatttga 229080gaaatacatc tgaaaaagaa atatttctgc cattatttgt atgcctaaac ttcaacaggt 229140atttagctgt caccacttac agatcttaag aaaatattca ccactattag agatataata 229200agatatacaa atgcctattc tttctaacac tatgtaataa acttttttgc tcacatatga 229260caagtggtgt tcctggtatt tggcagattc catttttcag gcaaccacag aggttgtttt 229320catgatgagt aaataaccta ccattgtaag ttcatatgca ttcctagaat agaggtagca 229380tcagcaggta ttcaccattc ctttgcatgc agctgttgtg caggtcaacc actaagacac 229440atctgacaca gggagtaggt aatctttgtc acgaaccctt cctatttgtt agaaatccta 229500cacaagttgt ccttttttat aaattagaga gaaaacctct ttcagtttac agatggattt 229560tttggagaaa gggataccaa atataaaaga cttactaatt gtttgtctag attcccatta 229620
tgtgtggttc agtttctgaa ataaatgtga tgataaaggt gataaacatg tctccagctg 229680tcagattaat aacaaaaaac aagtattcca aacatcacat actcaaaatt aatggccatg 229740actactgatt taatctacca aatataattt gtttaccaaa tttgccatat gctgttacta 229800ggtcagtaag attagatggg tagcaatttt ttgaactact tttgaacagt ttaagggtac 229860ttattcatag atcacatctg tttatctgtg ctctcccaag acagcattga tagttagaat 229920tctaaattgg gattttttta accctttttt caaaacttag ccccatctgt taatactgta 229980tttataaaac agagcacata atatgataaa ggaggttaat gattgaataa tgaagctttg 230040caagaaacta aaaagcatta tgttttagtt acttttattc atccaaagaa caggaaaaat 230100tatcttaaat attggcatgt tgcaattaaa catttagttc atcagtgtgc cctgtataaa 230160gtacttgcca tactctatta tttcaccatg taattactca gtttgcaagc atctaaccct 230220tgcacatcaa taactaatgt cacctctgtc ctgtggcctt gggagcctta attgtaaaaa 230280gtagacttct ggagaagctg aatacttggg agtagcagaa gaaaaaaatc aagaaaattg 230340gaatatatta ctcattcaac acatatttat tgaataccta ctgtattcct ggattcattc 230400ttggtattac atcagtgaag agtaacagac aaaaatacct gcccttgaca aagactgggg 230460cagattctcc agcactactg tcctgagcca tagagaaata gagcgtgact atacacactt 230520aacacatgca cggaagcata aaaaacattt tcctacatca tctgaatttg ttcacatcct 230580tttttctttt aaaaaataat aacatgcagt tatagccagt taacaaatct accttaattt 230640cctaggaact gtctttgaaa aaatataaat acttccagat aggtttatgg attttcagtt 230700ttttatacca cttgaaaccc ttcagtcaga ttgcttttct cctaacaact aactggtatc 230760acttgcacta agagtagaag tttttatttt aaggaggaaa ggtgatgttg aagtttgtac 230820catttacttg ggtcacagac agcctggctc taatgcctca aagaaaagca taaaatagaa 230880tgaaggagag acactgaatg aaggcagggt gctaaaaaca attaatgtcc atgcagaaac 230940atctaatgct ttgccttaga aaagggaggt gattcttctt ttcatcctgt ttttctgtca 231000ttctcctgac tccttgtttt gagggtccac agttcagact tttaccactc attctgagga 231060tccaccgttt ctgtggtttc caagggaaca caggctctga gttctaagca gctaagcttc 231120ctgaactttt ggagtgtaac ctgtttataa ataattaaat aacagcttca gggcattcat 231180gtgctcattg taaactagtc taatctgatc ataaaacaga gtgcctctta agcaactctt 231240tgttagccta attgtatgca tacacttaaa aattataatt gtgtctatgc ataaattcag 231300
ttctctaatt caacaaaacc cataccgttg ccctaaggat gcgaggtatc actctgctgg 231360aaatttagct ctactctaca tttaaaatac aagttttcac attaaataat ttcttattcc 231420ccaaaagatt aatttctgtc tctttctagt ccctcactga atttaaattc taactagtta 231480ttgcactagg gcagaggttg agatatcact gatagttcac actggatttg ttcaacctgt 231540tgttttgcgt ccatgaaaat agtttttggt ggtaatgttg tgtaatgatg tgtagaaatt 231600ttattagaaa tatttccccc ttctttcctt tattcttcaa gtattttagt atgtatgaca 231660aacaatataa attttgtagc tatgtttttt tatttcagtg tttagtggct aaattatcag 231720taagttataa ttttattaat aagtttcact gtaaaatgga ccatacaggt gatcgaggaa 231780acatctcaac atcttctaaa ccagcctcta catcaggaaa atcagagctg tcctctaaac 231840acagcagatc gcttaaacct gatggacgta tgagccggac tactgctgat cagaagaagc 231900caaggggcac agaaagttta tctgctagtg aatccctcat cttaaaatct gatgctgcaa 231960agttgaggtc agattcccac agtaggtcat tatcccccaa ccataacacc ttgcagacat 232020tgaaatctga tgggaggatg ccttctagct ccagagctga atccccagga ccaggttctc 232080ggttgtcatc tcctaagcca aagactctcc cagccaatag gtctagccca tcgggtgcta 232140gttctccacg ctcctcctca ccacatgata aaaatctacc tcaaaaaagt actgctcctg 232200ttaagacaaa gcttgatcct cctcgggaac gttctaaatc agactcttac acacttgatc 232260cagataccct ccgcaagaag aaaatgcccc tcacagaacc tttgagagga cggtcaacgt 232320caccaaaacc aaaatcagta ccaaaggatt ctacagattc ccctggatct gaaaatagag 232380ctccctctcc ccatgtggta caggaaaacc tccacagtga ggtggtcgaa gtctgcacct 232440caagtacttt aaaaacaaat agtctaacag acagcacctg cgatgacagc agtgaattta 232500agagtgtgga tgaaggttca aataaagttc attttagcat tggaaaagca ccactgaaag 232560atgaacagga aatgagagca tctcccaaaa taagtcgaaa atgtgctaat agacacacca 232620ggcccaaaaa agaaaaatcg agttttcttt tcaaaggaga tggatccaag cctttagagc 232680cagccaagca agccatgtct ccttctgtgg ccgaatgtgc cagagctgtg tttgcttcct 232740tcctctggca tgaaggcata gtacatgatg caatggcttg ttcttctttc ctaaagtttc 232800atcctgaact ttccaaagaa catgctccta taaggagtag tttaaatagc caacaaccta 232860cagaggaaaa agaaaccaag ttaaaaaata gacattcatt agaaatatca tctgcactga 232920atatgtttaa tattgcaccc catggaccag atatatctaa gatgggtagc atcaacaaaa 232980
acaaggtatt gtctatgctt aaggaaccac ctctgcatga aaaatgtgag gatgggaaaa 233040ccgagaccac ttttgaaatg tccatgcata acacaatgaa gtctaagtct cctcttccct 233100taactttaca acatttagtg gctttttggg aagacatctc tttggctact atcaaagctg 233160cttcccagaa tatgattttt ccaagtcctg gttcctgtgc agttcttaaa aagaaagagt 233220gtgagaaaga gaataagaag tccaaaaagg aaaaaaagaa aaaagaaaag gcagaagtta 233280ggcccagggg taatttgttt ggagagatgg cccagctggc agtaggagga ccagagaaag 233340ataccatctg tgaactgtgt ggggagtcac atccataccc ggtgacctat cacatgagac 233400aagctcaccc aggtaaatga tactgttgaa tgtacgtata agtgcttttt tctttaatga 233460accagatcac tttatttgag ctgttctatg aattttgtca aaggataggg aatttatagt 233520atcttagaat gtcattttta aagtacatta tcagtacagg gttttgtgaa aaacagataa 233580attctaatat tatgatggcc aggtcattta gtgctatttc actttcatgt agcatgtttt 233640aaaaaatatt atgtgttacc caaagggtgt gccattaatg tgtctgtctc acacagcaga 233700aacagtggca tgcccataca cacagggcat ctcctttgat tttactattt tcctgttcag 233760aattgcaaat taaattagat tagattgatt tctataggct ttttcctacg gaagtgatat 233820tgttttatca gaagactgca ttttcagtaa atgtaatgat ttctgtaaca ttgactaata 233880ttccaaattc acctaataca aatttggtta atttttttct ttgtctacat attatacctc 233940aaatgtctta tgaatatgtg atttctgaaa atatatcatt gttatagaac ttttatactt 234000gtattgtcta attttattga cataattaag tatatctaaa tatacctata ttctatctct 234060cttttaatat gcctgattag aaactaagta tctctcatat ttggggcatc atttaattga 234120ttgctttttg atcaagtaag ctatttttct gatatagtta atagcctgaa aaataagata 234180tctacttcag tattttgctt tctttgagaa ggtaaatctg tcattaatgt atgagtaata 234240aggatcaaac tattaaggac acttggagtg ttaaatattt atttttggta ttgtttcaag 234300gttgtggccg atatgctggt ggacaaggtt acaatagcat tgggcatttt tgtggaggat 234360gggctggtaa ctgtggtgat ggtggcatag gaggaagcac ttggtatctg gtatgtgatc 234420gctgtagaga aaaatacctc cgcgaaaaac aggctgctgc aagggagaag gtattttatt 234480ccatttggag ctatactttt aatgatatgg atacactgta taaaaatacc ttgttataat 234540cttactacat cagataattc cacatagttg atattaaaag atcagctttt ataatggaaa 234600tcatatacaa atttaatatt tgtactaagt caaatgcttt taattttaaa aattaactat 234660
atattggaat aacactagat aacctctgct acatatgcct tattgacaaa tatttgttgt 234720gtaactcttt attattaatt gtctgttatt ctctctatgg agagctttcc ttttcacctt 234780ctttctcttt tcctcttttt ttggctcctt tgttttccta ttccatatct actctctctt 234840ttctttattc actctagtgg cacctaacta aaaaatttca ctgaatacac gcactgaaat 234900gatttacttt ttaaaaaaat gaattacatt tagatttaac tagttaggtg ttaaattttt 234960ctgaagaagg aaaggaagta tagtttgtgt ttgatacaaa attgtcttgg tcagcccagg 235020atgaatatga aatgaatgtt gttttttata atcttattat cttaaaatag ctttacaagt 235080gtttaataat ttttattgga aactcaaaat acttagctta agaaactaag gattttaatg 235140taagtcagaa aagattagtt tgattttttt cttcgccagg tcaaacaatc taggagaaaa 235200ccaatgcaag tcaagacccc tcgtgccttg cccaccatgg aagctcacca ggtgattaaa 235260gccaatgcac tcttcctgct gtccctgagc agtgcagcag aaccgagcat tctgtgttac 235320catcctgcaa agccattcca atctcagttg cccagtgtaa aagaaggcat ttctgaggat 235380cttcctgtga aaatgccttg tctctacctg cagacattag ctaggtaata aaattttgct 235440gttgtttttt aatcaccttt ggattaacag cgattgttta ttgatatcta gggtagttcg 235500gtatttgacc tacactttat tagctattgt gtactataaa caaacttcca ggcatttatt 235560ttttgagttg ccttgactga cataatgact ttatggtaat aggtatttaa aaaactataa 235620aaatgcttca tcaatttgca ttgtattaat acacaacatg tcttagaaaa taagactata 235680gctatctttt actctttggt aggctgactt cactctgtta gtgcgaaatg ctgaagtgta 235740aagaaagtgt gtttccttag cagtctttta tttcgtaaat tattcattct gttagcattt 235800aaatgaatac caactgggca cctaccagcc ttgtgctagg ccctgaatgt atgaatataa 235860caaaatcctg gcttctatgt actcatactc ggatgtcaag tcagacatgt tagtgaaaca 235920tttgaaatgc catgtgagga gtgctacaat agaagtatgt atgtaagatg ctgttagagc 235980ataaaagatg gagagtatct gaatcttccc caggaaatca gaaaaggctt cccagagggt 236040ctgagccttt agtagagtct caaaggataa atagaaatct gtcaggcaag cgacaggaag 236100aaagcgactc ttggaagaat aacccaggtg agtaaagaca caaatgaaat ctcattacac 236160atttcaggaa tcacgggttg ttaaaggact ggctactgga ggaaatatgt gaaagcccgt 236220tcaaagtgat tggttcaccc aagtaatgtc gggctctgta gcccaggtta agattgcatt 236280ttatcccatg agcagaggaa acagtggaag aattttaaac agagaaatac atgaactggt 236340
ttgcaattaa aaaatcactg tggaatgtgg aagctaatgt ggagactaga taaaagtgca 236400ttactggaga tagaaacctg ttagtattct gttttcatat attggacctc agtggtgcag 236460cctgtacttt cacacatcat gcactgacac tccaaaagag cctttcatat accaaaacca 236520aatttttttc atcagctgac aaatgaaggt aatctagata ttgcccactg acatggcttg 236580ccgaccaaca gttccatttt ggaaattgtc ctctttgtgt agcagagttg tatggttttt 236640aaaattttgt gcttactatt acaataaaat gaataagcaa caaaaatgaa aatatcagac 236700taagtacatt tgagactgag gtggaaatca tcagtatgcc aaaatacaat agatctttag 236760tgtgaatcac accactcagt ggacttaggt tagcaactat ttgttcattt tgaaaaggaa 236820agaatcaaat tgtaaaacgt gaaaaatgct aacacagcat aattatagat ttttttcttt 236880ttagctgtct ggggaatggg caccccttcc accctgaacc acagaaaatt tcatatatct 236940aatatttttc aaatatgatc ttatgtatgt tcctacccaa aaaagtaact gcttgtattt 237000cagtggtctg tgcaagaaat aataagcatg aaccaaggca gtgatgatag ggaatgagaa 237060gagagaatgg ctctgagagg aaacctaata tcatgggctt gaaagatgat gatgatagag 237120ggctaagcct actacaacac tggaaatatt ttagcttaag caactggatg gttggtgggc 237180ttattcacca aaacactgga agatggaaaa gttctcaaaa ggaaagaaac tgagttcaat 237240tttggatttg aaatgttcgt ggtacctgga atgttgatga ataaaattat cagactttta 237300ttttcaccta cctacatcta cgattatttt aatgctttgc ctttaactat gatttacatg 237360tgctgctttt cctgttatga aagagattaa aaatggagag gatctgtatt gccatcatgc 237420tttaagttat tgggtttgat ttaatgcagg catcatcatg aaaattttgt gggctatcaa 237480gatgacaatc tattccagga tgaaatgaga tatctacgtt caacatctgt acctgccccg 237540tatatatcag taactcctga tgcaagtcct aatgtatttg aagagccaga gagcaatatg 237600aagtctatgc caccaaggta ttttagttct ctcctgtctg ttgaatagct agtgtttggt 237660ggaagaaaga ctttgaacta gaaaagagtc ctgtagaagt tctttaatac atctgtgagc 237720cagttttgct aactgctgga gcagtaagaa aaagcataca tgtatgacta aggtaaggtc 237780cttgatggga gaggcagact agttggagag aagagatcaa ctagtgaagg aattaaagat 237840tacattatat gacattactt aatgctgtag taaatcagag tagggaaggg ttactttgag 237900ttttcatggg agcagtggca ttgaaaataa atcttaaact tcttggatta ttgtttacag 237960tgcctcctaa cttaggtatg gtagtatcag agtaaagagc ttctcggaaa gctgaataga 238020
aacttgttac aataatgtaa tacacaagaa aatagaggcc aggcgctgtg gctcacacct 238080gtaatcccag cactttggga ggccaaggcg ggtggatcac ctgaggtcag gagtttgaga 238140ccagcctgac caacatggtg aaaccctgtc tttactaaaa atacaaaaaa ttagccgagc 238200atggtgacac acacctgtaa tcccagctac tcgggaggct gaggcaggag aattacttga 238260acctgggagg cagaggttgc agtgagctga gattgcacca ctgaactcca gcttgaggaa 238320aaaaaaaaat agagtaccac tgaaactttt ttagaggacg aatgatgtga taaaaatgca 238380aaggtagtct cacagtgaca attgagaagc tgacatatgg attaaagaga tatattttgt 238440aggaaaaata agtgtgactt gttgactaac tggataatga aaaagataag tttaagatga 238500catgaagatt tcagtcacga ataactgtga acatggtatc attggaaagg aagacatttg 238560ctgaatgaaa agagaatgtg cttattaaaa gagcatgcca ctgtgttact actatacaac 238620tataattata aactaatata ataaatactt taatgagcgc aatgggacat atatatccaa 238680atgaggtttt tttttttttt cattaaaaat acatgcaact tctcccctcc ccaactgtgt 238740ggattactcg tcagaatttc tttgaagctg ctcttgacat tttctccaga gtgcagtgca 238800accttttata tctttaaaag tgataaagta tttctaaagg agatacactt tttaaaagag 238860ttagaaactg ttttgaggct agtctgacaa gtgtgattct cattgggtta ctattacccc 238920ttaggatcaa aatccaggag gctgaggcaa gaggatggct tcagcctagg attttgaggt 238980tacagtgagc tacgattgtg tcactgcact tcagcctagg tgacagagca agaccccttc 239040tatttaaaaa aaaaaagttg atgaataaat gaattcatta aatcagtggt tctcaattga 239100gggtgatatt tgcctctctg ttgacatctg gaaatatttg gaaataactt tgtcacaact 239160gggctagttg agtgggcatc ctagtgagtt gaggccaggg atgttgctaa ccatcctaca 239220ggaggcaaga cagcctaaca aggaattacc tggctcaggg tagccattca attaaagatg 239280tcatctttgc tttcttcttt ggattttctt gtgttgcttg aattctttca caagcaattt 239340tttttttttt ttggtaaaaa gtcacttttt aaatttgtgt aatttttttt aagttagtag 239400aaaacttcct ctgaaaacag aacaaatgtc tgtgctttat agtaacacca tatataaagt 239460gaattaagtt aatgtttccc tgtattctag tcattctgga accaagcaac ctatactaac 239520acccagggac atttactgcc aatcagttat gtacagtatt tatagtctat tcaaacaaga 239580atgtacataa gcttagagaa gtggtgagta aagaaaacac ctaggcagtt agccgtctat 239640tagaaaatag ataattttac caagggacac aggatttcaa acctggcttg aatgatggaa 239700
aaaactccag aaccaacggt gagcaaaaat attccaattt gaattcaggt gacagaaaaa 239760ggagctcacg aaaagaatag tttctaaatt tgttttagag aaataagaaa gagaagggat 239820ttctcttttt ttataaaatg tgtcaataaa ggaaattgta tagctctctt aataactttg 239880taagtatttt taaatgttgc tttaataaat agaaaagctg ttagtttact taagcctgaa 239940taaaaccatt aagttctttt atagagtgta aatacaacac tggtgccaaa aatcagtttg 240000ttacctctca tgacaccttg atgcagccat cctccaaggg ctttgggcaa catttcccaa 240060gagtgagaaa cctcacagaa cactgaagac accaaaagaa aactttggaa ccaaattttg 240120cttcctgttt tattaggttt tcatgtgtac atatccattt tctttctatt tggtgcactt 240180acgattatat ctgactgttt ttcctgaata ttatgataca tgaattccac cccagtgctt 240240ttgattctaa aaggagcttg gaaacctttc ttctttttca tttaaaatac aaagtagatt 240300taagaaaacc ataagtttta aggtacaatg ctgaaatcag tcaaaatgtt actgcatttg 240360aaaagatcaa attatgattt aaagagagat tatcaatttg gctttgattt tgtttctcca 240420catgaaatat tgagtcacat attttgcatc attataagta tagttacatt tcattcagct 240480cttctgagtt tgtatctgtt gcattgttcc atgtacagtt tagaaaccag tcccataact 240540gatactgatc ttgcaaagag aactgtcttc caaagatcat actcagttgt tgcttccgaa 240600tatgataaac aacactccat tttacctgca cgagttaaag ctattcctag aagaagagtt 240660aacagtggag acactggtaa gtatgaggat aaaaaagaat attgagtgat ctgactacta 240720caaataaaaa aattgtttta tttgcatata atttttaaaa tttaattttt gaaggaaaag 240780gcatgggata atttctaatg tgtgattgac attccttcta tatgacatag ctataaatgt 240840gcatctctaa attttgcatc ttagttgaag ggaaatctgg agatcattca gtgattgttc 240900gagaataaat tgacctttac aatcttactt gttaggtatc taagccaaat gttacaacca 240960ttatcccttc cactaaactt ttgtggacca ggagttccaa tgacgtacac attcacagtt 241020atctctccac cccaactgaa gcttgataac tcttggagct gccatcattc taagtagata 241080ataaaacttt ggggatataa atagccctta atcgatttaa aaaaaaaaaa aaaacagtta 241140catgatctgg accagttggt atcaacagcc ccaaattgga gctggggatt ctacctcaag 241200aataatttta aaaacccaag aagaacaggg taatatagtg atcaattatc agagagatca 241260cttgctaaaa tgaagatttg gcctaaaaat tatagtcaga tgattaagtt atcttactga 241320agacttttaa gaaaaacatt attgaagatt gtccagcaat ttataggttc attcaacaaa 241380
tatttttgac ctcctgttaa gaaatgtttc ttataaagcc atcttttaga aaatgatgca 241440gatgagatgt agatttcatt tagagaaaat ataaatcact tagcttttag agtgttttag 241500aaaaggtgaa ctttagattc agctctgatt caaaacccac ctctacaact taatagctat 241560atgactttca gagagtaaca tatttaatct ttctgaacct tggtttcctc agtaaaagtt 241620gaaattgtta tgatttgtgt catgttcaaa aaaccaatga caaaggcttt aagtgaatat 241680attagatatt attatcttaa atatatatgc actgcctatg aaaattaata attttctttt 241740tctttaatag aagttggttc ttcccttttg agacatccgt ctcctgagct ttctcggcta 241800atctcagccc acagctctct ttctaaagga gaacgaaatt tccagtggcc agttttagct 241860tttgttatac aacatcatga tctagaaggt cttgaaatag caatgaaaca ggccctaagg 241920aaatctgctt gtcgagtttt tgctatggag gtatgaagac attaatgaaa ttactgattc 241980attgattata caaatcatgt gatgattcta tttcacgatg ccactttttt accaacagaa 242040attgtgtgaa attaaaaggc attagccaat taaaagtatt aagtgttcct ataaaaatca 242100gactctaaag tttatgaata taaatgaatt tagaatttca tatttaagca ccctaaagaa 242160atttatttct gtattgcttt actatttgaa tcttgagatt atcttttttt taaaagcttt 242220ttatactcag aatgtcattt tattagaatg aaatgacaga gtaatcatgg aaagaagata 242280aggagtaggg cttttgagtg gtaagttgac cagtaataac ttctctgggc ctttggtctc 242340tggaaataat aggattactg tatattacag gcctatatat agtaaatctg tgtttttatc 242400aaatgtgata aataacctac cacttcatgg atgggaatta tatgaatata tttaagtaaa 242460tggttgcttg cctagagctt gagatacact ttgtgttcat ccagatgcaa gttggaaaaa 242520atgtgcttga ataaattaat attaaaatga attttcaaag taaaatgtga tttgaaaaaa 242580tattttggta ttgtatctta taaattattt ttcctgtttt ttttaaatta aaaccccaaa 242640ttattaactg tttcaagttc cgtgtgcttt cagtgaaata aaggtcagat tttcaaggtg 242700ccaagcatag tagcccatgc ttgtaatcct agcactttgg gaggcggagg agggaggatc 242760acttgagcgc aagagtttga gaccagcgtg ggcaacatgt gagacctcat ttctacaaaa 242820aaaaagtttt aaaatcagct gggtgtggtg gtacacacct gtagtcccag ctacttggga 242880ggctgaggtg ggaggatcat ttgtgcctgg gtgcttgagg ctgcagtgag ccatgatcac 242940accactgtac tccaacctgg gtgacagagc gagacactgt ctcttaaaaa gaaaaacaaa 243000tagtaaaact ataaggcatt ttttttaatt aaaaaagatt ttctagtatc tttctttgaa 243060
gtcttaatcc atggaggtac tttatttctg agtacttttt aaactcatgt attttattct 243120tgaactcatt taaccgtcat ttcttcaggc tttcaactgg cttctgtgta atgtcatcca 243180aaccacttct ctccatgata ttctgtggca ttttgtggca tcactgactc ctgcaccagt 243240ggaaccagag gaagaagagg atgaagaaaa taaaacaagc aaagaaaatt cagaacaagt 243300aaatgaaatt tttgttttga ttgtgcatat ttttatagaa actggtagta taccttcaaa 243360tagtccaata atttgcatga aatcattcgc agatctaact tgaaagcaaa aagaatattt 243420tagtgctatg ggcaaagtat taataatgag acctggattt tatagaaggc agcctgtata 243480atggagagag cattgaatta gggcactagg agcctgagtt cagcctcatt tctaccctat 243540tgtagttgta tgacttgtag gcctcggttt ccatgtctgt aaaatgaaag taatctaggt 243600gacatttatc ttattcccag tcactctgaa attatgtgaa tggcatttta ggttaatttc 243660caaggatatc aagcaacatt atcatagtca aattcttaaa actcacaaac aagacaccat 243720gagcaagaac taacaaaaac aatagacagt agaaatagac cctcaacaat ggttgtaatt 243780atctatgtaa atgtagaata atgtgttaat atgcttaaag aaataaaaga gaaactttaa 243840aatatgagaa gaaattgtaa gaagaaatat gaagtgatga atttgataaa gaactaaaga 243900aaacataaat actaatctaa gtataattga tatttttagt tttgagattt tatagcatgt 243960cagtgaaaaa attggctaaa tataagagaa tgatggattc gataaagaac taaagaaaat 244020acgtaaaaat taatctaagt ataattgata tttaaaaaac aatcagttgg acattttata 244080gcatgtcagc aaacaaattg gttaaatata agagacctgt agaaattatc tagacttcaa 244140cacagaaaaa gtaaataaaa aatacaagtt gctaagagca atgaagggca aaatgatgta 244200caacttacct acttggagtt tcagaaggag aagacagaat ggaagagaga cagtgtttaa 244260gagttaatac ttgagaattt cccagaattg attacagata acaacccatt gagtcaagaa 244320gcccagtaaa ccccaagcag aataaataca aatcatggtg gacaaattat agtgaatcac 244380ctgaacacca aaaacaaaga aagagatctt ttaagcagcc acagaaaaaa atattagctt 244440tcaaaagagg gacagtgaga ataattgctt aatttttggt agcaacatta caagttaaaa 244500tactgagttt gtttaaaagg ctgtcagttg gtttttttca acgtgctgaa agaaaatact 244560tgttaaccca gttgaagggt ctttgaagaa caagaccaaa gacatttcca gaataaaaac 244620tgggagaaat ggccatggag tatcaatgaa gagaaaagaa agtgtaaata tgagggcata 244680tcttaaaaaa cattgactat gtaaaaagta atgatatgtc atggtgatgt acaaaatgat 244740
aaaagatcaa tcctggatat gtatctacct aatactgtaa ccttaaaaaa agtaacaatt 244800gacagactgc aaggaaaaat aggacagtct acagttacaa tgtgagattg tcatatacct 244860gtaacagcta tctgttcctg cataactacc ccagaactca ctggactaaa acagttgttt 244920atttgctgac agtctgtggg ttggcaattg cagcaaggct ccactgagag gaatgactct 244980ttcctgtttt ggttacatag tgtgcttcaa gtggtacccc ctgagctctt ttatcctcca 245040gaatgctggc ctaggtttgt tcagatgcca gaagcattca agaaagagag cacagaagct 245100gcaaagccaa aatggcgaac actaaactca tatgccatgc ttctgccacc ttccattagc 245160tagtataatt cacaaggccg gtcaaattca aagactgaga aataaactcc acctgttgat 245220ggaaagagcg acagagaatt tgtggctgtt ttgtgtatag taccataaca ctctttttag 245280tactggatag gtcaggcaga gaaaaagaat cagaaagtac acagatttga atgattgaca 245340gtcttgatag aatatacaaa acattgcata tagttgttta cattcttttc agatacacac 245400aaatgtctat ggaaattgac tgtttactgg acacataaag caagttttaa caaacttgaa 245460ataattggtt ttcttcccac catgtcctca gtctttattg ctagtatata agaattagat 245520aacaaaacaa taacaagaaa aatatttata gggaaattaa gtaacgcttc caaatgagtt 245580gcatcaaaga agaattataa cagaaaatat tttaaatgga atggcaataa tagtacatct 245640caaaacttgt tgtttggcag agttctagtt atagttacga tgaaacagcc agtgtcagca 245700ttagttctta tagtaagtaa caaaaagctg ggcaaaatat ttaaaacaat tgttcatagg 245760ctctgaggag tgaccagtac agggctgtaa tccttgagag aagaaaaggg catgaggtaa 245820gccccacaat tgggaaaaga gcccaagcag agtacgtcag ttgtgttggc agagatcaaa 245880gtttaggact gtaactttgg gatgtgggaa gcagggcact agtggtacag agctgcagaa 245940agggagtcta aaatttttgc ataaaaatat ccctagggtc ctggccactc ttagactggg 246000catgcaattt ctctggagga tctagcagtg gaaatgaatt gtaagctgag aactgaacag 246060attcttagct tcagagctgg gttctgaagt tttagtccaa ccagaggtgg ggtgagcgtg 246120atgaatactc ctggcatgcc cttgaaaccc cagaaagacc atactcaagg aatacagacc 246180atgccttagg agtaagggca gtgcctaaaa tcaagggcaa aactgaaata accccagtct 246240aacaaagtcg aatgccaagc cttgatggaa tctagatggc ctgctatctc cctgtaagaa 246300caaaactaca ttctttagaa gacaatataa accagaactt ctatatcagt agaagataat 246360ataaaccaga acttctatat cagtaatcaa tatatgcaca aagaaggagg caaaaagcaa 246420
ccatctttta cttagctcag tgacatagtt taaaaagttt gatgggctta cattggtagc 246480aagatggaag aacaaacatt ctttgctgtg gagtagcagc ttcttagaga atggtttggt 246540aagatctatc aaaattcaca ttaaaaatca ttcagtaatt ccacttttat gaagttctcc 246600ttatcatata tgcatgtgtg aaaagacaga tatacaagtt gattcattgc agaatgttat 246660ataaaaacaa aagattggaa acaacctaaa tgactgttct gaagtggact gatgaaataa 246720actatggtat ttttattcat cagaatacta tgcagtggga taatatatgt tacatacata 246780tctaataagt aataatctca aagataaatt attgttaaaa gaacacagat cagtttgttt 246840ccagtgctgc catttgtgta aatgaacaat atttatagac agtccaagaa actggtaata 246900gaaattgcca gtgggagggc atctaggtgg ctgggctgta gagacaggga gactttttat 246960ggtatttttt tatacctctt gaatatttta ttatgatgtg tgttatctac tcaaaataat 247020aaaaagttat tccatagtta atattattaa tggagccatc tgagggttgt aatgagaaat 247080gaaattcaac ccctacttct ctttcctagc tgtggtccat ttgcgtagaa aggagctttt 247140ttttctaatt cacataaaaa tgctgtatga gttagtgacc accccaactt ctaaacatat 247200gttatttttt atacataaac atacacacaa aacataccta catatatgta tgtgtatata 247260tccatacaca tacatatata tgcacacaca ctacacatac acatgcatac acatgtggat 247320tcaccctaca ggtatctttg tgagtacact agaattgata catttattca tttgcatgct 247380gattttgctt gtgactaaac ttacagcttt tttccctaaa ataatacaag cataattagt 247440taaaattact gtatctttct gggtacaatt tggttttgtt gtttgtattt ctgttacatg 247500accatttttt aagtatacat aatagtcttt tgtgaagagg attccttcca gcacacaaac 247560tgataaactt gtttcaattt ctgccttagg agaaagatac aagagtatgt gaacatccac 247620tctcagacat agtgattgcc ggggaagctg ctcatccttt accacacacc tttcaccgct 247680tgctgcagac catctcagac cttatgatgt ctctccccag cggcagttca ttacagcaaa 247740tggccctgag gtaattttgg tatccacata tcctaggtac attggacaag agagtttatg 247800gaatattcaa agtatgaaat gctccaaaaa aagctcttta aatagtaaga tagaatccct 247860acattatata gatggtgcct ccctccttac agtcataatt tttagaatgg tccttcctct 247920tagttaacat ttgtttgccc tcctgtaaaa tgtggctaat gtaattctta gtgccctaaa 247980ttatcactgt agcaatctcc ctcactttcc ccagtctcct acctcaaatc acttaaatgt 248040cccaaaaaga gagagggaaa atgaaatctc aaaaacaaaa accaaaatag agttttgact 248100
tgaaaagaat cttggggaaa acagagcctg gttgtatgca cccagtccta aaagcacctt 248160aaggagcatc acagatgctc aacagattcc aatttcaatt tgaaatgtgt tgagaattta 248220gcattgcttt tacactgtga accttgttac tagattcaca tactggactc acacttataa 248280ataaattctc ctaattaatg tatacaagaa atagattgat ggaatgcttg ggcttcccat 248340gttactaatg ataagaccag tggaagagag atttctcttt gtcctcctca caagattttt 248400gaactacaag ataaactata ccctgatgaa ttagtatggc attgtaagag taataaattt 248460gaggggttgt tgcagttggt gtaccacttg tttacacaat agattcatta ccctttttgc 248520ttctgtagcc ttgatcttca tctgtctaat tcttttatat ttaaaatggg tcaactttcc 248580ttagaattta ccttgttcca aagaagttac ctttatttct caaaaacaaa aacaatagga 248640atgattttct tccctgaatt tttaaaatgt ctgttactag aacattaaaa tttcttgaga 248700tattagctac agagtactca tctaagagtt ccttagatgt tcctggaaaa taattatttc 248760gtatattgct tatatatcgc ctttttaggt gctggagtct caaattcaag caatctgatc 248820accagttcct tcatcagagc aacgtctttc atcacattaa caatattttg tcaaagtcag 248880atgatggcga tagtgaagag agttttagca tcagtataca gtctggcttt gaagctatga 248940gtcaggtcag tcatttcagt tattatcctg gttgtcctgt tagttaataa tacatcaaag 249000gagaaactgt aagttattca taatcaattt gatcctgtct tccattatgt tttatcttat 249060acctgtttgt tttatcatca cgaaagattt gcttttaagt atcataagta cttatatttg 249120tttctgatag gaattatgca tagtaatgtg cttaaaggac ttaaccagca ttgttgacat 249180aaaaacttca agccgacctg ccatgattgg cagtttgaca gacggctcca cagaaacctt 249240ttgggaatca ggagatgaag ataaaaacaa aactaagaac atcaccatca actgtgtaaa 249300aggaatcaat gcccgctatg tgtctgttca cgtggacaat tcccgagatc ttggggtaag 249360aaagcaaacg tgatttacgg ctctttagct cttttcagat cttagtattc atgcactttc 249420atttctgaat ttagcaacaa gctttatcac ctggaatttc accaaccaaa catcattgct 249480ataatcccca aataaatctt aagtctaaaa acaaattaat atgattggga gcactgtgga 249540attccatttc tgaaaagagg tctgattaca aatataccat ggctacagct aagcaaaatg 249600aaagtctgct ctacaatttt ctgctgccat tcccttggtt aagattttcc cgtgacctgg 249660aagtcctccc cttaaactct gctgatcaat tcttccaggg tctctcaaac tcagcagaga 249720aattctaagt aactcctcta aaaaattact ctttccctct gctgattctt tttgtattac 249780
tctggtggca cttaataata atctttgacc tatagtgtaa tcagcttttt tttttttttc 249840tgagatggag tctccctctg tcacccaggc tggagtgcag tggcacaatc ttggctcact 249900gcaaccttcg cctccaggtt caaacgattc tcctgcttca acctcctgag tagctgggat 249960tacaagcaca cgccaccatg cttggctaat ttttgtattt ttagtagaga cagggtttca 250020ccatgttgtc caggccggtc tcaaacccct ggcctcaagt gatctgccca cctcagcctc 250080ccaaagtgct aggattacag acatgagcca ctgcacccag cctatcttca agtgtctact 250140ttgccacagt cctcatgaaa taaaaaataa gtttcagact gtcccaaata atcataattt 250200atattctctt cagcctagtt catccatgtg tttcaactga attaaatatt cttttataaa 250260aggcactaaa caagaaaaaa gagtcctatt tgcttgagta ttaacaacca agaaagaaat 250320agcactaact gccagactgc ctttcttcta cctcatttag aatttttttc ctcagaatgg 250380atgtggcttg ctagcagata cagtattaat ggctctcctt gaaccattgc ctggcttctc 250440ctgtgccatt tgcatatttc ctttctcagg aagagccaca gtggtaggat tgggtctcag 250500ataacctgga tgcaggccag tctgcaatca gagcaacctc caactcctgt acatcagtgc 250560aggttctatt cctccctggt attgagtaag taaaagaggc ttagctaata taatatgagc 250620tatttcctcc tcagacctaa gaatttccac tggtgtaact actttatctt actggaaagt 250680caagtgtaac aaagcaaaac atttttgtag ctagaactca ctgttcagtg caaagctatc 250740tttggctcat ggtacatttt aagcaaggac atactctccc tcagaacaat gattgcaatc 250800aatttaacac ttctgtcttt tctaagagga aataatcata acagtattca ttttcaaaat 250860tcccaacatg taagctgaca tctactttcc ttttaacagg cttcctctat gttataggtt 250920gagcattcca aatctgaaaa tccaaaatgc tacagaatct gaaacttttt gaacacagat 250980atgacactta aaggaaatgc atattggagc atttctggtt ttagatattc agatttgggg 251040tgcccagcca gtaaggataa tgcaaataga gtatttcaaa atcctaaaaa atccctaatc 251100caaaacactt ctagtcccaa atatttctaa taaaggatac tcaacctgta tgtatgtttt 251160ccttgagtta tgtacctcca tgttcctcaa cctgcctacc acagagcctt gtacacagca 251220tttgttgatt tgagttgtat cagcctacag cactcatatt atgcagctct ccaaggaatt 251280aatgttaatc tatttcttga tgtgctttgc gtaaaactta ttcagcctta agatatttga 251340cactagtttg aacatgtatt gaacttgtac tgtttgacat ttattttgtg tattaatgtt 251400gccttcccta gtttggcaag tatcttgggg gatagaacct cctctcatat atcctacttc 251460
aaatctgtaa ggaagtctgg gtacattgtg acactcataa gtatttgtta gttcagttac 251520cttccctctt tatacttcca aaagtaatga aaatatagca acaagagtct gacataagtg 251580acaagcagat ttgttgaaag gcttcatgag gaaggaatct gaatatttag actttgaata 251640aggagaggga atgtcaagct gtgagagagc acctggagag aggctgaaat gtgcaattta 251700agatggagtt tgtgagaatg agactaaagt tataggaacc gtggttagtt ggagggatgg 251760ggtggggtca atcaggaagg accataagag acaggcagaa gagttgatgt ggtattagta 251820ggtcatgaga aaccattgta agttctgaag caggaatata gcatattaac tattgtgaat 251880tgtctatttc agaataaagt tacctcaatg accttcttaa ctggcaaagc agtagaagat 251940ttgtgcagaa taaagcaggt aattgaaatt ttgtcattaa atgtttcgct acctagagag 252000aaatttcttc ttctcagcct tcactattga attccaccct tggcacacaa ccaaagtatt 252060atagggaatt atctatttac agaacactaa attgacaagt ggtccaaaac ataggaatag 252120taaccaccat ccctgtgctg gacatctcac ctgttttcct aaatgttccc cagtcatgag 252180caattatatt gtacctacag tctccctacc ctactcccca acaaagactc agggtatctt 252240aaaatttatc acagatccca ttcacactta aaaccttact tcacctatac tttgagttaa 252300tacaggttat tgtcctctgg cctgaaaaat ctgtctgtta taacacttat aacactgcag 252360ctatatccag gttgcaagtc agtttaaacc aactttagaa acaagttaag ttttatctag 252420gcatgtgtct gagactgtgc tcaagctccc ttacttgtat tcatgaaact ttgtcaaatc 252480tgttcctcac tggctacttt atctcacaaa aagttggcca atcgcttgct tgcttgcttg 252540ttttcaaatc acattgaagg aatcaattaa gattttggac taaacatata ataaattttg 252600tttaaattgt tgaagaattt gggaaaataa tcctattcaa tgagattgtg tttgtttata 252660cttaataccc ctttttgaaa attgacatta ttaattaaaa taatatcaac ttaatgtcta 252720tttaaatcac atagaaatcg ccaggtcatt tcttggttat taaatgccat aagaacagtt 252780tttattttat gaagggaata gtgtcaagaa ctatttcaca gtatattaac atttaatcct 252840tgaaaaaaaa agtcatattc tcaatgaaaa ttagtgaaat aaggaatcac aagtgtgaag 252900ttacatatta tgtaattttt gttcaaaagt aaaacatatt aagtaactgc actttaaagt 252960cccttatctt taaatggtac tttaatgtga ggatgcacaa aaaaagaacc cttttctgat 253020cttgtgaggt ttccacttac agatgatata gataaagtac agtcatataa attatttgta 253080attatttcac tcatataatt ttgctcggaa gccatatcgt agtcaggcct ggattttaat 253140
tctcaattcc accatttact gaaaatccct taacctgtat taaccttagt ttcttcatct 253200gtgtaatagc tactataaat aatattactt atcttcacct ggttgaaata agaaaatcag 253260tgtgaaataa cctcataacc tttactctgt gcaattcttt gttatttgag cttgctggta 253320tcaatgctaa gtccagcaca gtgataaaag tgatccagct gaatcaggtt cctgcctctt 253380ttctactcaa ccaggttgat ctggattcca ggcacattgg ctgggtaaca agtgaacttc 253440caggagggga taatcacatc ataaaaattg aattaaaagg cccagaaaat acactgagag 253500ttcgacaagt caaagtcctg ggctggaaag atggtgaaag cacaaaaata gctggccaga 253560tttcagccag tgtggcccag cagaggaact gtgaagctga gactctgcga gtattcagac 253620tgattacgtc tcaagtgagt gtccttacaa catattctag cacaggataa ttgatgtaat 253680atattttaag agtgtaacaa aatattttgt gaaacttatt acagaaattt ctggctataa 253740atgttgccca ttttttcttt tcatagccct aaacacctga gttccatgtt gcatttgtaa 253800ttaagaaaaa tagtggtgta tattagcaag agtagcatat tataaaacct gcggaatcag 253860ctaatgagtc ctctggccaa tatagtgtta atgcctgtca ttaattcctt ctcacatgca 253920tatcttcatc aggtatttgg aaagctcatc tctggagatg ctgaacctac accagaacaa 253980gaggaaaaag cactattgtc atcacctgaa ggagaagaaa aagtatacaa tgtatttatt 254040tgtattctaa agatatttat ttcccttttt tattcttttt ttcagtgaaa taattcattt 254100attgctgtat attttgatgg atatgtaata acagttttat gcagttcata tttgttctta 254160gaaagatgaa ggaactatct aaaagaaatt cattattcat gtataatcta gctagtattt 254220taagtaacag caaatttgct ctataccttg ggtttttgca ttcatttgta atcatttctg 254280tgctataccc catcagttat cttctccctc ctccattgtc cctgtccttc acagttcagc 254340tcaaaagtac tgtcttcttc atgaaacttt tttacgattt atcctcttca tgaaatttgt 254400ttatgatttc cccaaacaaa agcagttttc tctctgctgg agttcataat aatctgtatg 254460tgtctcttct tcatttttct cttatgttta tctacatgga tgttttctct cttctactga 254520accatagctt cttaaagacc aagtccatgt ctaatttgtc tttgtatcca tcatggtagc 254580atcacacaca tgtttagtaa atttttatta gaataactac tctttgttac tgttcttgaa 254640atttcttgca aaaatacagt tgctttactt tttcggtcct gactaaaaca ctatacctaa 254700aacttttgta cttcatccat ttgcaaaaag agaagtcaac tctgactagt ctcaatattc 254760tagtgtcttt aaatgctaca aataatcacc ttggcagccc tttgaaagat aaatttcaag 254820
gatttccctt gtctcaccct gtgtttctta atacagtagt atctaatcat tgtcctgttt 254880ttgtttgagg tctgaaaaaa ataagatttc ttaaatgctg gtaacttctt tcaacatctt 254940ttggagattt catttgaatg actacaagac tgtgtaacta cctgttaagt caattgaatt 255000ttagcagatg tcaactctgt actaaacaat tgtgagaatg gcaagccctc aaaaagcttg 255060cattctgttc ttggttatga accctctgca ttcccctcac cccccccaaa aagaaatcaa 255120caaagatact atatagtata aaagcaagtt attaaatgtg tgaaaaaaat tggatcattg 255180tgccctggag tggtcagaga aggcttctta ggggatattg gactggagtt agatctgaaa 255240gagaatgaag ggattcaaat gagattagta gagagaatgg gagcaaggac tgatcagaga 255300gactggccta agggtgggag agtgtatgct gaggagttaa ggttgagtag gaaaggttat 255360actgtcatgt gtgtgcccat gaaataacat gatttttaag agaatctttt tttaagttag 255420attattgaag cacaatttac atacagaaat tcctcatttt aaactgtatc attcaatgaa 255480tttttacttg tgtacactag tgaaatcatt atcacaatca agtttgtttt gttttgtttt 255540gcttgagacc gggtctctct ctgtcatcca ggatagagtg cagtggtatg atcacggctc 255600actgcaacct caacctcccg ggctcaatgg atcctcctgt ctcagcctcc caagtagctg 255660gaactacagg tgcacgtcac cgtgcccagc tgattttaat atatctgcag agatgtggtt 255720ttgccatgtt gcccaggctg gtctcagact cctgggctca agcagtctgc cagcattggc 255780ctcccaaagt gctgagatta cagatatgag ccaccatgcc cagcccacaa ccaagtttta 255840aaacacatca ctccccaaat ttctctcatg ttcctttgca ggcaatcccc caacctcaca 255900cttagagcct agcaactact gaactatatt ctgccagtgt tgtgttgcct tttcctggat 255960gtcatataaa tggaaccact cagaatgtag cctttttgtc ttgtttcttt cactttagtg 256020taatgctttt gagatttatc catgttgtag catgtagcca tggttcattc ttttggttgc 256080tgagaagtgt tccattatat ggatttaccg taatttgttt atcctttcat tttttgatgg 256140atatttgggt tgtttctgct gtttggctac tattagtaaa gctgctgtga acactcacat 256200acagatcttt gcaggtcttt taccaattat gtgttttcaa atattttctc ctagtctgtg 256260tcttatcttt cagagaacag aagtttttaa gtttgataaa gtccagtttt ttcttgtttt 256320tttgattcat gatttttatg tatggtctag aacccaaggt cacacagata ttctcttctt 256380ttcctttaga aatgttatag ttttagttct tttctttaga actgtataca ttttaatttt 256440tatgtgcagt gtaagaattg aggcttatct tttgcatata gatgttcaat tgttctacta 256500
cgattcattg agaagactat catttatcaa tagtcaattt accataaatg gtaggtctgt 256560ttctggactc tattctgctc cattgatcca tcaaagtgtc ttacttactg tagctttata 256620aaaagtcttg atatcaggtt ctaactttgt tctttatcta agttattttg gctattctag 256680atctcttgca tttccatata aagcttagaa tcagcttgac cgtttcttta aaaaaaaaag 256740gggggggggc ggtggggatg gaattttgac tggaattaac accaaattga tagtttaggg 256800agaatcgcca tgtcaataat attgagtctt cgtatctgtg aacatgatat gtatcagggg 256860ttttcttaca tctgtttaaa atttctctca gcagtgtttt gtaattcatt gtacacatct 256920tgtacatatt ttattaaatt tattcctaaa catttaacat ttgtaaagct attgtaaatg 256980gtatttttta tttcactttc caattgttca tttctagtgt ataaaaatag aattgacttt 257040tgtatattag atttctaccc tgtgatctta aattcactta ctaatttcag aaacttttta 257100tagactcttt ggggttttct tagataatca tgtagtcttc aataaatttg ggttttttcc 257160tttctaaact atatgcctta aatttctttt cttatattat tgtgttattg cattgattaa 257220gcccttaagc gccatgctga atagaagtaa tgagaacaga tagctctgac ttgttctgga 257280tcttgaaaag aaagtataaa ccattcatta ttaagtgtga tgtgaactgt agattttttg 257340ggggtgaatc tttttaataa gattggagaa tgttttcttc tattcctagt ttgctgagaa 257400tttctatcag aatggattct tttttaagag acctggtctt aactctgtca cccagacaag 257460agcacagtgg tacctttata tgtttctctt tcttcatttc ttttattccg taagataatc 257520catatattgc tatgcatttt agtcaacatg tgatactagc ttgtatacag ttcattttcc 257580ttcagtgaaa aaaaaaacta ggggatggta gctacatgat atagtgcttc ttttagaagt 257640aataaaaatg ctgtaatatt gactgacgac gatagttgca catatctgaa tgtactaaaa 257700accattgaat tctatgcttt aaacagatga atagcatggt atgtgaacta tatctcaata 257760aagatgtttt taaaaagcaa aaataaatga tgaaagaggt aaacaagatg aaagaactat 257820ctaaaagaga ctcatatata atctaactag tacttaagta gcaaaataaa tgctctattc 257880taaaaattca ctttctttaa tagataacag agctgtttca gttatctgtt tctttttggg 257940taagctttag tagttctgac actcaagcaa tttgtccatt tcatctaagt ttctggattt 258000attgccataa gttgttcata atataccctt atgtcttttt aatatctgta gaacctgtag 258060tgatgtgtct tggtgatttg tgtcttcatt ctttttttct gaaatagttt ctctaaagat 258120tcatcagttt gatctcttca gagaactagc tttttgtttc attgattttc ctctattttt 258180
gcttctttta ataatcacaa ctttatttct ttccctatgt ttgccttgta ttcatttgtt 258240catctttttc tgacttctta aggtccaaga tcagatcgta gactccaaga cctttctttt 258300ctcatctaag tatttaatgc tattattttc ttctaagcac tacattagct gcatttcaca 258360aatttggggt attctgtttt tattttcatt caattcacaa tgttttctta gttttcttat 258420tcccatttga tttctttttt tttttttttt tctttttttt tgaaatggag tcttgccctg 258480tcgcccaggc tggagtgcag tggtgcaatc tcggctcact gcaagctctg actcctgggt 258540tcacgccatt ctcctgcctc agcctcccaa gtagctggga gtacaggcgc ccgccaccat 258600gcccagctaa ttttttgtat tttttttagt agagatgggg tttcaccacg ttagccagga 258660tggtctcgat ctcctgacct cgtgatccac ccacctcggc ctcccaaagt gctgggatta 258720caggcgtgag ccactgtatc cggccccatt ttgatttctt ctttgaccct tgggtaattt 258780agaagcacct tgtttaattc ccaaatattt gggaattttc ctgttagctt tatattatta 258840atttctggtt cagtttcatt gtggtcaaac tgtattgtat acttcgtata atttcagtcc 258900ttttaaaatt gttaagattt ggattctggc ctataatata gtctgtttta gtgaatgtcc 258960acgtgcactt caaaaaaatg tgaactctgc tgttcttctt tgaggggttc tataaatatc 259020agttagttca agttgatcaa taatattcaa gtctttaaga gccttaatat tcaagtcttt 259080aagagcctta ctggtttttg gttttttgct tgtttacttg ttctgttaag tactgaaaga 259140agagttttga agtatccaac taactggatt tgtttatttg gaattcattc tacctggtag 259200ctgatctcaa cattctaatg gactccaaaa aaaaagtttt ggttttgtag atgatcaggt 259260ttttcctcat tgttagggcg aaagggacat tctcttctgg ctctctatct taagaaatgt 259320aaaaccaagc atcatttttg attcttaaac aaaagagttt cttacctcag ttttcttacc 259380tgtaaagtag gcaaaatatt acaatttacc ttctggggtt gttgcgaaga ttagataaga 259440tttgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg cgtgtgcaca cacgtgcatg 259500catatatata tatacacaca catatataca tatgtggcac ttagaagaac agcgtgcaca 259560taataagctc tataaaattt tagcctttat atttttcctt atgatacatt tctttgatga 259620cttggtgttc cgatagctta atttaagacc actatcatca cagaggtttg ttaggacact 259680ttccttttaa acaaatgtta ttttcctgga ttgttcttca aacaattaaa tgtgggatgt 259740gaactaataa tgtttacaat ttagaaatag atttaatcaa agctactcaa attgactaca 259800ttaaagaaat ttgtgaaagg agaatatact tctctgaatt tcctaagttc tgagattttg 259860
ttctttttat ttcaaacttt atcctttgtc ttagtattat aagcctcaat ttaaaataaa 259920tttactttaa aaatatatac acaaaattat ttctcaagtt actttgaaat atcacatata 259980caaattttat ttaaagagac cacttcattc tttttttttt ttttcttttt ctatgtaaag 260040gcaacatcag atgctgacct gaaagaacat atggttggaa tcatattcag caggagtaag 260100ctgactaact tacaaaaaca ggttggttaa cagctatttc attgtcttca ttagttttgt 260160gtgtgtgtgt gtgtgtgttt gtttgtttgt ctgtttgttt gttattaggg tataaagcag 260220ggatgtccaa tcttttggct tccctgggcc atatttgaag aaaaagaatt gtcttaggct 260280acacataaaa tacactaagg ctaacaatag ctgatgaact aaaaaaaaaa ttgcaagaaa 260340aaaaaatctc ctaatacttt aagaaagttt atgaatttgt gttgggccac attcaaagcc 260400atcctgggcc acaggcagtc ccccggctgc aggttggatg agcttggtat aaagtatttt 260460gaaaaattta tgaaggcttt tttatgctcg cccttttata ggtcctgcag aatgtgcctg 260520ccatttttta gagaggaatg agtattttgg cataccttca tcattttaaa gattttactt 260580atgaaaactt gcttaccttg ctgctatttg ttatgggtag attttttatt tggtgtcaaa 260640tacccagcta tatcagattt gatttgcatt tcccatattt cttttccccc attcttctgt 260700gtgttggctg agcaattaac agtgttaact ataccagtgt taatattttt aaatataacc 260760aggtgaggaa atagcgctat attgttcata taatggggtg ctacataaca taaaaatatc 260820acttcaaaag tcaaataaaa tattgatttt aaattaacct attattctaa cttttttttt 260880tttcgagagg gagtctcgct ctgttgccca ggctggagtg cagtggtgcg atctcggccc 260940actgcaagct ccgcctcctg ggttcacacc attctcctgc ctcagcctcc tgagtagctg 261000ggactacaga tgcctgccac cacggctggc taattttttt tttttttttt ttttttttta 261060gtacagacgg ggtttcaccg tgttagccag gatggtctcg atctcctgac ctcgtgatcc 261120acccgcctcg gcctcccaaa gtgctgggat tacaggcata atatttttgt atttttagta 261180gagacggggt ttcaccgtgt tagccagaat ggtctcgatc tcctgacctc atgatccacc 261240tgcctcggcc tcccaaactg ctgggattac aggtgtaatt tttttgtatt tttagtagag 261300acgggatttc accatattag ccaggatggt ctcgatctcc tgatctcatg atccgcccgc 261360ctcggcctcc caaattgctg ggattacagg cgtgagccac ggcacccagc cctattctaa 261420ctttctacac tattattctt ttcaatgaac tacaacatga atttattttt gctttatctt 261480tctagcagtg tttcattgaa tcttagatgc tttcaattgt aaaatgtcat gtataatttc 261540
agtattgtga atcttatcag ctataaagca ccttatgaac ataatggtaa accatgagaa 261600aaagtgcctt cagtgataaa atatggtgat tggtttttga cgtgctaaac tgtaagcata 261660ggcattaata acttgcatat aatcagtttg cacatagtag ttgatttaaa tatttccatt 261720tttattgtgg ctcccttagc tttacttgtt tcttagatga gttaagagaa tgacatatga 261780tggagaatta actgggagaa tagtattaaa ttgagtatca atttacattc ttgtcttatt 261840cttttgtttg cttgttttgc taggagtagt aaatcaaact aaccctatat ttttacatgt 261900tctgtgttaa ctttaaatag gtgtgtgctc atattgtcca agctattcgc atggaagcta 261960ccagagtccg tgaagaatgg gaacatgcta tatcaagcaa agaaaatgcc aattctcagc 262020caaatgatga agatgcctcc tctgatgcct actgctttga gctgctctct atggttttag 262080cactgagtgg ctctaacgtt ggccggcaat atctggctca acagctaacc ctgcttcagg 262140atctcttctc gctgcttcac acagcctctc ctagagtcca gagacaggtg actgatcaca 262200ctacagcctt tccattgctt ttgttacttg aaacattgtt aaaagttgca atgcaaagag 262260aattatctaa ggaccctaag tatagatttt tatttcgtat tttgttatat tttgtttggg 262320gcctgtattt tactcccttt tttactgttt atagtgagag tatagaatag taaaatatgt 262380tccattttat taatgtgttg tgataagaga gtattctggc tactgattac catattacca 262440tgaaatatat aatgtcattt agccctgacc agaaagaaaa ggtatttttt ttcgtgctgc 262500cttgggttag taattaccac tactattacc atttgaatag tatgtatatc atattttctt 262560ccttcacaaa atgtaaaata gtgtttaaat catatttctt ttcttaaaga ttttgaagtt 262620acagaatggt agttaggtac attaaaaaat gaagcctggg atggggtaca gtgacttatg 262680cctgtaatcc cagcactttg ggaggcaaag ctgggaggat cccttgagcc taggagttca 262740aggctgcagt gagctgtggt tgctccactg cactccaacc tgggcaacaa agcaagaccc 262800tgtctttaaa aaaagaaaag agaaataaaa gaaaccttaa aacattagtc tttacatgga 262860attctctcat tttatttttc ttaccaggta acctctttac taagaagagt tttgcctgaa 262920gtaaccccta gtcgtctggc cagcatcata ggagtgaaat ccctcccccc agcagatatc 262980agtgatatca ttcactcaac agagaaagga gactggaata agctgggtat cttggacatg 263040tttctaggat gcattgccaa agcactcact gtacagctaa aagccaaagg aaccaccatc 263100actggaacag ctggtaccac tgtgggcaaa ggagttacaa cagttactct tccgatgatt 263160ttcaattcca ggttagttat tgcctctatt ttagtaccaa aacagaatag agtgagctac 263220
tgttcagaaa tttaagacat aatttcagat tttaccttgt taggttactc accaaataaa 263280atccataaac aactggctaa tatctaaaaa ggtcatgtat ataataaaag tgtcatttat 263340aattagtggg gaatagaaag taggctgaat atgtgatgtc agaatatttg gatatccatg 263400ttaaaaaaaa ttctagatac ctacctctca ccatatacaa aattaaactt cagttttagg 263460taatatgttt ttttgccaca ataaaaagaa ataaatatgg gggaataaag taaagttcag 263520gtcaactaaa gctttatgta catttctttt taaaactgta ctgggacaaa ttatagaaga 263580atatttttgt aatcttcaat ttgataagac cttcttaaac aaaacacaga acccaaaatc 263640atataggaag atatttatag atttgactat ataaaaatga aaattacttg ataggtgata 263700atatttaaat gttttaaaat atagggaaat aacttagaaa aatacttaaa cctatataat 263760agacaatgac ttgttagcat aatatagaag tatgactaca aatcaatttt aaaaaggcaa 263820acaactgata agaataataa atgacaagaa cagtcagttc acagaaaaaa acttataaat 263880gccagtagtc agttcacaga aaaaaactta taaatgccag taaacaaatg aaaacacctc 263940actactaatc agagaaatgt agattaaaat aattgcaaaa tatctttgat gctttggcaa 264000cattatgagg aaataggcat tttcatctca tataaattgc tgcagccttt ttggatatct 264060gtttataata tctaactata ttttaaatat gcatatcttt tgacctacca attctacttc 264120taaaactttc atgcatttgc accaaggtat atatgcaagg atgttctgta attgtgtgta 264180acagtagaga attagagaca tctgaacagt gctacttgta gagccaatcc atgacaatat 264240gcagataaca tcagaatgtt aatcattgta tcacttcttt cctgtagtct tgctaggaaa 264300aaagtcagct gaactaaata gtagtgtgct tagtgataca gtgaatttat attccggtta 264360agctccacat tatccgcatg tgccctagaa ataagtcgtg tgtggtccct ggactgtact 264420tctaaaagat gcacacagaa ataatgtgca aacagagaaa tacttgaaag ggaactcaaa 264480taaccttaac tgctgtcttg ggatttgggg taatggtttg atgaattttc tttttaaatg 264540ttttaattga ttcagtcact taaaaatcta aaatgctgca ttctgaaata acattttcat 264600ctttttcatt tgttactact actgataatt tattggcttt ttaagtgcta atttttcttg 264660tagttatctc cgacgaggtg aaagtcattg gtggatgaag ggctcaaccc ctacccagat 264720ctcagagatc atcattaaac ttatcaagga tatggcagca gtaagttccc attcttctgt 264780tttggcagtg aagtgcggca ggaagctgac acaaaacagc ttactctgct gaattgatag 264840gagatgtaga ttagtgtaac tttacttaaa gactatttgt agtatgttaa ctatcatgct 264900
gtttgtcttt tcagtgagtg ataatttgtc atttatgtaa accatgttca tttcaaattt 264960tccagttaat ttgtgtggga attgttacgt gactttaaaa tctaataaaa taaaatatgc 265020tctttcagac cctaagaaag aacttgattc aaaaaaccta aaggaaaaaa agaggaagag 265080agctctcgaa agtggaagtt tctcattctc ctcagtcctc atggttttta aaccctttat 265140tcttctttat taatgtcata taccctctgt cttaagatgg tggcttccaa aggagatctg 265200ggagtgtgga ggacagggag ttactttaca gaatgcctgg ctctgattag ttgctccttg 265260tcattttcca cacattgtat tatttctagg agggcagcct gagaaggaca agatagtatg 265320gaatgtagag tcagaaggaa tagaaggcag cttggagaag gaggaggaat tgaactgccc 265380cttaaatcat gtggatatga gaagtgggag gattttctgg gggaaagatg aagttctatc 265440acttttccca acagcgtgga tattttctca acctctgtgg caaacagact ttctcagggt 265500gttcattgaa ttaaaatatt taggagtaat atttaggaac acctaaatta tatgatagtt 265560aaatgtttgt tcattacaaa tcaaggtagc aaataaaggt agttgcagtt tgaacctgtt 265620tatattttag gttcctgtaa tactcatcta aagcagttag cttcactgtg taaattttat 265680gctaagtgac aaacttctca aacatagata accttagtat ttctgttatg tttttatcag 265740atgtgagagt aattgccctt gatttccagg aaaagagtat tgagaactta attttttatt 265800tatttatttt taaatatcaa aatgatgatt agaagttgaa ttttccatag gatcacgaag 265860aaattttagt tcaaaagttt gtgcccataa tctaaaattc catggtctca ctaaaaacaa 265920atatttcctt tgataggaga tgttatttaa gaaaaaagag tttactatgg taatacattt 265980ctggtcactt attaaaatac tctgttctgc tcaatagggt catctgtcag aagcttggtc 266040ccgagtgaca aaaaatgcta ttgcagaaac catcattgcc ttgaccaaga tggaagaaga 266100atttaggtct ccagtgagat gtattgcaac aactagagta ggttttgctt tgttttgttt 266160tgttttaaat ttggtgtgta tcattgcgtg ttctttttta gtaatagatt taaacaaatt 266220ttaatcacta agtcaacata attatttttt aaccaaatac cgtatttatt gaaaaagtat 266280aaagggcttt tatattatga actagtcgtt acttctcact gttatgtgtt ctgtgtcact 266340tgtttttgtt attgtttagg aagccaaggg tacactggtt agaaaaggct aattacattg 266400tacattgttt gcgtgttgtc tttctaatga tctgtttgct gtagtttacc acagtaagtt 266460tagtgccagc attatgctgt gatagaccga aggtcctttg gagacaagac ccctgcctat 266520gcctggttgt tcctaatgta gagccctgta acttacttac aggatttata ggtcttactt 266580
ctaaaaagca agaagtgctt gtccagggca gggtcagggt ggaaatcttt gaggaaatga 266640gattgcattc ttcagatgac acatttatag taagttttaa cctgctagat ttcatgtccc 266700atatacttcc agaagagatt tgagatttcc ttgaaggagg gcagtctgac aacgttagga 266760agaagtgaat gctggtaaaa attgcttagg ttaatcctca gaaatctgaa gaagataaac 266820tatatatccc tagggcggaa aaagtcaatt tatgctatct tgctattatt gactgtgaca 266880cctgaaatag gtatttttga cagtaatcct ttcctaccac tcatgtgttg ctttctttca 266940gtctgcattc cccttcctcc ttgcatgcat tatttctttt tctatttttc tgtccctatt 267000ttaacatctc ttgctttctg tatccaataa cactcacatg cacttcttag agttgagcta 267060gttaagtcat tttctattct tcattaatga ggggatatat cattctatat aaaactgaag 267120cttacccttt aagcctcaga aatttttaac tatttctgaa aacagttgtc tttttttttt 267180tttttttttt tttgagacag agtttcactc ttgatgccca ggctggagtg caatggtacg 267240atcttggctc actgcagcct ccgcctgcag gtacaagcga ttctcctgtc tcagcctccc 267300aagtagctcg gattacaggc atataccacc acacctggct aatttttttt gtatttagta 267360aagactgggt ttcaccatgt tagtcaggct ggtcataacc ccctgacctc aggtgatcca 267420cccaccttgg cctcccaaag tgctgggatt acaggcatgt gccactgtgc ccggccctat 267480tttggagaac agttttctaa agcttttaga catgtatgta tttctgacaa agtgcaccaa 267540gtatagtatt aggctttctg gggcaacaag gggcaacaaa gacttctgag gaacttttat 267600tatctagaag gatttaatgt gtacctgact actcatggga ctgtacacaa tttagcagtt 267660gctgtaccac cttctttggc atttaatgat actaaatgga aaaatattca gaagacatta 267720gtagtaaata ttaaatatgc caattcattt attcatatgt tgtttacata gtagaccaat 267780aaaggataat tttatttgtc agatagaaga cagggtggtt catcagagaa acagtcactt 267840gttcagtaga tatctgagtg cctcctgtgt gcatggcaca taggacagtg atcgtgtatg 267900cctgctacca catcaggaag acttgagtgc cactgagtat ctatgtattt tcaaaatcag 267960ccagcattta ttgagctgtt gtgatgtgtc acagtcagca tttcataagt ctttcctaaa 268020aattcagtgg tgtaacagcc ttccctttca acagctctgg cttgctctcg catccctatg 268080tgttcttgat caggaccacg tagatcgtct ctcctcgggg agatggatgg gaaaggatgg 268140acaacaaaaa caaatggtaa gatcagaatt ttgtaagaat ttatcttgct ttcctttaca 268200gtaagcttta gcttagctta aaaacaaaca aacaaacaaa aaacacatca acataaacag 268260
ttagtggatc tcttgaaaga ttgtgtccct tcggccaagt tcatctcaca agtctatttc 268320agagagtccc tacttcatag tatatggaaa ctttaggggc ttttatttaa gtatattaaa 268380gagctttctt cttctctgag tcatgtttgc atcatatcaa aatttatttt tagccagagt 268440ctgatttagt gactagatat tttactaaaa tgtaatcagt cttatggtag gaaggaaata 268500aagcaataac acaggcaaca tgacacacca cctgttcaca tatatactta agtaggtttg 268560atgggtatct ttaagttcac tagaaaggaa aactttatat aggccctttc accattttct 268620tattaatgtt caagtttcaa aaataaacag tttcttcctt tggcatacag aatgtttaga 268680tatataatgg ggcagacatt tcttaagtct ctaagtaaca aagtatacct gttcattgta 268740ttaatcattg aacctgcaaa gcacattaat gattggattt ggagttttga gagattttta 268800tagttaagta catttgcaat aattctgttt aatattcaga ttctaaattc ttaatagact 268860tccgaataac ggtttatttt tgtaaataaa ttatattcaa tttgtatgag agtctgagac 268920ttgcttgtta atctaagtaa cgtggaaatg tcttatttta gcctatgtgt gataaccatg 268980atgatggtga aactgcagca atcattttat gcaatgtctg tggaaattta tgtacagact 269040gtgacagatt ccttcacctt catcgaagaa ccaaaactca tcaaagacag gtgaagattt 269100ttgtctctgg agcataaaat atgttcagtg aattttgaaa taaatgtgtg aaagaggctt 269160tgtgtagtag ggggaaaaag tggactttgg aatcaaagac cttggttgag ttcacagctg 269220tctcgcttac catctataaa tagcaagttg ttttatcctt agaaacactt aatttctcca 269280tttataaaaa agggactgct gtgaagatta aagagaagat tggaaatatt caaaacttct 269340tagagtgctt aaatgaaagc cattattatt attgaataat taaattttat actctccctt 269400tcagagtgat aaataagcat atttttccct gttctttaat gaaataatag gtcttcaaag 269460aagaagaaga agctataaag gttgaccttc atgaaggttg tggtagaacc aaattgttct 269520ggttgatggc actggcagat tctaaaacaa tgaaggcaat ggtggaattc cgagaacaca 269580caggtaaaag gtttaaagat tgagccatgc ctttaaaacc cacaaaaaaa gattaaaccg 269640tgccaactga ttaaactgtg aataattttg agtgatattc tgatgtttaa ttatgacatc 269700attgatctat atatttacta aatatataga gtgttctatg gtgaagagag cattagacca 269760aaagtcaaga aacctgattt gccactaact agctgtgtgg tgtcgagcaa gtcacttaac 269820ctctggaact cattgacttc atttataaag tgagaggatc acaactaatg gggctccata 269880gataaaatac atttctattg ataattccct tctagttctg ctagttgttg ccccacaata 269940
aaaattgctg cactagatga tcactaagat ctctatttat tataaggttg tatgatttta 270000tgatatttag aggtttttgt agacctaatg cttctgaagg tgttgtgaat ggtactgttt 270060ccattgatag caataaaata attaacaaaa atgaatagac ataaaacaaa aggtaaccta 270120tgtatgttaa atgtattttt atctctaaaa catagttttc attttgctgc ttttctaaag 270180taaatcttgt atattcctag ttcaaagtta tgttgaatct tacaaattct cagttttcag 270240cacatctgca gtctgccatt tgactgacac gaacttccag tgttggagtt aggtgtaaat 270300ccgaatcaat ttaattctag ggtgactttg catgctgcaa gagcatgacc ttcttttctc 270360atagttgatt tcttcctcct ataatctgtc agtgctgtat actgcattaa taaatgttct 270420aatgtcaaac catccttgta aaccatcctt tttaaataga tttttctgta aatattgctg 270480aattcaatat attgatatta attatagtta gatttttata tctgtgttta aaaatcagct 270540tggtctgtaa ttttcaaatc ttcacgttct ttttcttatt gctctgttct tgttttattg 270600attctgtttc ttctttaagt atttcaaaca tatttaaatt ccttttcaaa ttgcagagtt 270660cctgaggtag gaattctgtt tttcaagttt catactctga tgataggctt tataccatgc 270720cttataattt tgttatatat agttaaagat ttccctaact tctgtgtatt tttatttaat 270780ttttggttat catatttttt atggtattgt gcatttggag tcaggggctt gtaacagctg 270840ctcagtctgg taacttgact agaagtctag tctgtttctt tcactgaaaa aaaatattag 270900tcgagagtgt atttggcttc agataggtct ttaatttctt tttttgtgtg tcattattta 270960ctttataatt aggacaactt agaaaccttc cgttctaaaa ctgtcaggct gatttcccaa 271020ggatagcaac ttatacaaac ctagtttgct ttttaaaatt tagttctgtt aacatatcta 271080gaaaacatcc ataagaagga tttttatttt tctgcttgaa cctaggcaaa cccaccacga 271140gtagctcaga agcatgtcgc ttctgtggtt ccaggagtgg aacagagtta tctgctgttg 271200gcagtgtttg ttctgatgca gattgccagg tgagtatata gtgacacagg ccatcccatt 271260gtccatgttc ccctgtgaca cctttacttc atacagaaaa gagttttagc tcagcgtcac 271320tccagcttcc taaatgagtg tattacctat gtgaagcatc aaatgcctta ccaaagttga 271380agaatgggtc aaataaggct ctcaagccca aaccacactc tttttgtatg tttctcagtt 271440accatgatca tatgtattta atatctactt ttgtactaga aacagttaaa aatttatgaa 271500ctgtatttcc tttatctctt acagacatta ttcacttatt tccttaaaat acattttcaa 271560catctaagag ttctttaatc ctgaacatat ataaacatat tctatgttgt ccaaatgttt 271620
ttggtttctc aattaataag aatgtggata tgctcagaga gtcaaaggta cctttttaaa 271680aaaaaaaaaa atctgcattt tggtgatgcg gagagtggtg actgggaaat tatacattgg 271740ggaacttttt ttatttttta tttttttgag acggagtctg gctgtgtcgc ccaggctgga 271800gtacagtggc acgatctcgg ctcactgcaa cctccgcctc ccgggttcaa gcgattctcc 271860tgcctcagct tcctaagtag ctgggattac aggcgcgcgc caccatgcct ggttaatttt 271920tgtattttta gtagagatgg ggtttcacca tattggtcag gctggtctca aactcctgat 271980ctcgtgatct gcccacctca gcctcccaaa gtgctgggat tacaggtgta agccacgact 272040cccagccagg aactttttgc tttatagctg tgcatgatga caaatatgaa attataattc 272100acttatcagc attcccttta tagagagtgg cattttttaa aaatcgcatc aaaaaaaaaa 272160aactttgtta agaagcctaa tttatattca aacaaagatt agaaataact ttactttgtt 272220gttacttggc ctgcatagat acttcctgtg ggaaagtaat ctttggaaat ttatataata 272280aggaaaatta ccagaatgtg tgctttttta catacttgtg taacattgta attcatcttt 272340aacaggaata cgctaagata gcctgtagta agacgcatcc ttgtggccat ccatgcgggg 272400gtgttaaaaa cgaagagcac tgtctgccct gtctacacgg ctgtgacaaa agtgccacaa 272460gcctgaagca agacgccgat gacatgtgca tgatatgttt caccgaagcg ctctcggcag 272520caccagccat tcaggttgcc tcagctttta aagtattgaa tgtatccaga cattaagatg 272580gggaatatat ttgtcaaata aacattgatt gaggccgggc gcagtggctc atgcctgtaa 272640tcccagcact ttgggaggcc gaggcaggtg gatcacgagg tcaggagatc gagaccatcc 272700tggctaacac agtgagaccc cgtctctact acaaaaatac aaaaaaatta gctgggcgtg 272760gtggtgggtg cctgtagtcc cagctactca ggaggctgag tcaggagaat ggcatgaagc 272820caggagacgg agcttgcagt gatctgagat ggcaccactg cactgcagcc tggtgacaga 272880gcgagactct gtctcaaaaa aaaaaaaaaa aaaaaaagca gttgattgaa atcttttttt 272940atatcactag gcagaagtgt tcttttaatc tatacatgta ccaggctaat gaggagcaaa 273000ttctgcctct ttatgctgtg ctttctatgt tgcatgctca gctttaattt gtgatttgtg 273060tgaacctctg taatcaacag aatgtataaa atattcagaa gccaggggga aataattaga 273120ttatccccca gtagctaagg ctcttcttaa tttccaaaat ccagaatggt atttttacaa 273180cattatataa ttgatctata cataatgata catacttcta tacatacaca agttactgtg 273240cactaaatag gtttttttaa cttttgccca ttgcttatat tttcagttct catgattcca 273300
agataataat agtctccaaa agaaaatcat aagtcagtaa tttcttcttc tttctgtgag 273360atacaaaatt gaataatttt actttttttc ctaacctagc ttactttata taagtggcat 273420gaatgttaaa aagcaggaga agccaaaggc ctagttaata gtatcccaat ttactgtaat 273480gaaatttagt aaataataat gtcccaattt cagtgtccca atgaattttc attatagtaa 273540atttcttgat ttttctctta cctgctttaa gtagtgcctt gcattaaata atctcccagt 273600cactcaactg tttatcatta ttatatttgc ttatgtcctt ttcttttgtt tatttaagct 273660ggattgtagt cacatattcc acttacagtg ctgtcggcga gtattagaaa atcgatggct 273720tggcccaagg ataacatttg gatttatatc ttgtcccatt tgcaaggtat ggaaagaatc 273780tgaaatcatg tactttcttt ttcttttgtt ctttcttttc acctttcaaa ataaacatat 273840gtcagtatct cttggtttta cccaccagcc cctagcagtt tcttcccttc tcattcatgt 273900ggaacctgag aatataatct ctctgttata atgcatctca aaacagtaat tgtccattta 273960aaactgatta cattctgtta aacatattac atgagaaagt tcatttaaac atcgtgatgc 274020attaaagggc atgcatctct actctcagac cactagagag ctcagttgac ataacatgac 274080aacggctacc aaaaagaaga atgatcctct ggtgtttgta gaaactgata cctgccatta 274140aagagtgaaa ttggaaccct cagtaccact agaacagatt cttttagagc ttagttaaaa 274200caaggagaaa gttcttggca ttttgacaca tttgtacaaa ggtagtcagc aagtaggaag 274260ttgttttggc aagctaataa tgattaacaa aaagctgttt ttgaagaatg tggcagtact 274320ttccctatta attcatcgcc cctttttgtt ctattaagct ctccctattt tcttatcttc 274380gtatttgcaa actaatataa agggtagaat gtggaaagac ttagcacaat cattgtctag 274440atagactgag gaataatcaa aaagattgta gtattttgct ctcccctatc atatggtgct 274500acaaatatta atagatacat gaaagtttta cagcaaatac tacctgttca cttctgtcac 274560ctggcgttct gccttcccct caaggaagca aaacacacac acacacacac acacacacac 274620acacacacac acacacacac acacacacac accaaacaca gagcgtgtct tatttgtggc 274680aacaaggtaa ctttgtttcc acaatagcta gggcaacagg agatatattt cagacgcagg 274740aaatataaag ctaataaaat ggaattttca tgctctgtgt cccatttgcc ccattttccc 274800tgctcttgga aaaatatggg cttctaaaga atttacagaa tgtttttcaa atgacatttt 274860atttagaata tgtgtgctgt ctggttatct tctagcacaa tgcttggcac ttaggtgcat 274920gctaagtgtt tgttgagttg gttaataaat gattctgcta aagagtgttc attttttatt 274980
gcagaacaaa attaatcaca tagtactaaa agacctactt gatccaataa aagaactcta 275040tgaggatgtc agaagaaaag ccttaatgag attggaatat gaaggtctgc ataagagtga 275100agctatcaca actcctggtg tgaggtttta taatgaccca gctggctatg caatgaatag 275160atatgcatat tatgtgtgct acaaatgcag aaaggtatgc tataaattat actgagaagt 275220tttaaaaact agagcttacc tatatgatta agaattcaaa ttgtacagtg atatctaatt 275280attccatctt aagcctgaag ttaaaaataa gatagcttgg tacaatgttt cccaatgttt 275340ccagtaaaac ttgtttactt aacttgtttg gggattcttt atttaacctg aacaaacttt 275400tcgaaacatg cagtatttct gtatacaagc tgcttcccat cagtaataac ctgttggccc 275460agggcaattc cttctcgaat aaacttctct cacttgactc tgctaaccat tccttcctta 275520aaacccttaa ttccttggct tccaggacaa cacactcttt ttctgtcctc cttcctttct 275580agtcatttat ctttaactac ttcttctttt ttttttttta acctacccct tacatatttg 275640ttttcctaga gctccattct tgcctactta ataaatactt ccttaccttg ggtgacctct 275700tttacatcta taccttcacc taccatgtat atgtttatga ccccccaaaa tttttgagat 275760ttagttacaa ataaccaact gcgtaccaga catttggatc tctcacacat acttcagagt 275820tcacatgtca ggtattatat tcatcaatct tggccgggca cagtggctca cacctgtaat 275880cccagcactt tgggaggcca aggtgggcag atttcttgag ctctggagtt tgagaccacc 275940cagggcaaga tggcaaaacc ccatctctac aagaaataca aaaattagac aggtaggttg 276000gcatctgcct gtagtcccag ctacttgggg gactgaggca ggggaatcgc ttgagtccag 276060ggggttgagg ctgcaatgag ctgtgtttgt gccactgcag tccagcctgg gtgacatagt 276120gagaccctgt ctcaaaaaaa aaaaaaaaga ctttaccaat ctctgatctt tattgacaaa 276180atcctatcaa ttctgtctcc agatctctct tgagtccttt tttctttcca ttacatctcc 276240agcaaaccta atcattttcc ttggtaatta cagtagcctt cttcctgagc tcgggtgtag 276300aacccctaat tcctctgttg catcccttac actgctgcta attaatactt ctagatctaa 276360aatctaactg gatcactctt ctacttataa tacggtagcc cagtctccct ctccagtctc 276420atctcccaag atgctaccat ttgctctcca tttgctgcca cataccaaat aacttgcatt 276480tcccaaactc agccctgtct gctcacccac tgacaccccc atgccattgc acaggccatt 276540ggtgtttaaa ggatgctctt ctctacctcc ccctgcatat tttactctga cctgccaccc 276600ccaaccccat acacacatgc tgtcatgcag agcctaatct gtcttgtcat acctcgggct 276660
tcagctcagg tttcacctcc tctgtaacat acccccatct cacttccatc aacaccacca 276720ccacatgatt ttggtatctt gcaggcatct agcatagaac tccctattct gcattatgac 276780tactggacca cttatctctc tgccctactt gataagttcc atgaggacaa agagtatgtt 276840ttttcatttt tctatctcta gtactttgca cttactagat acaaagttaa gtggagaatg 276900aataaatgag tgaaagaatg actacatgag aaaggtgcat agtctcccaa tgtagcaaaa 276960agaaaataca gaggatgaat ggatgtctaa gtagagagat gatagccaga aaggcagata 277020aatacatgca cacccaatag tgcacattta tgaagcttgt attgttacaa ataataaaat 277080agtatttctg tgtgtttcat gtactaaaca ttataaatga atattgtgct attacagggt 277140agcagtcaaa tgcttaggaa gccaagcaga ggatatagaa aagtgaaaca gctggagtaa 277200gcagtcccca agacaaaccg aaatacttcc agggttaggt ttagctggtg aacgaccgtt 277260tataacctct gcatcaatgg atcctgtctt tggacctgtt tttctagtat agataataga 277320tttatattga ctcagtttgt ttattcccaa tccggacata gctttttgac tatgagacaa 277380tcagtgcatg caaattgtcc cagttccttg gcctcactta atctctgccc agggcaaata 277440cagataaatc acccatcatg atatttttat ttattcatga taattttatt tattcaaatt 277500ctatttatgc aagtgtctgt attggaaact gttgagttcc tgcctactgc ataactttat 277560aactatgagc agaattctga acagaattat caaatttgcc tttttttttt ttttgagacg 277620gggtctcacc atatcaccca ggctggagtg cagtggcatg atctcagctt actgcagcct 277680ccacctcctg ggctcaaaca agcctcccac ctcagccttc tcagtagctg ggaccacagg 277740tcctcaccac cacgcctggc taatttctgt gtttgtttgt atttttgata gagatgggtt 277800ttgccatgtt gcccaggctg gtcttgaact cctgagctca aggaatctgt ctgcctcacc 277860ctcccaaagt gctgggatta caggtgtgag ccaccatgcc cagccggcct tttcttctta 277920aaaaatgtgt gatcagagga tgcttaaaat tacttttttc tttcccatta gccttggaat 277980tataattcta tactaattac tttatgtata ttttgttcta cagtattata aattgggaac 278040tgctaagcat atttattaaa acagtagaag gggatatgta ttttaatatt tcaaaatgag 278100aaaaatttct caaactttac cctgaaaaaa gcatagcttt tactacttaa tattctgttg 278160atatttactt ggaatattat acttttttgt tttgacagga caatgtaact gcctacttta 278220agcttgaatc catttgagag tcgaaatagc tttaggccag gcatgtggct catgccaata 278280atcccagcac tttgggaggc caaggcaaga ggatcacctg agcccaggag tttgagacca 278340
gcctgggcag catagggaga ctctgtctct acaaataatt aaacaattag ccgggtgtgg 278400tggcgtgcac ctatgatcct agccactcag gaggctgagt tgggcggatc gcctgagccc 278460aagaggttga ggctgtcggg agccatgatt gcgccactat gctccagcct gggcaacaga 278520gtgagagccc gtctcaaaga aacagagaga gaggtggaag aggagaagag aagagaaggg 278580aaggaaggga aggagggaag gaaggtagga ggggaagaaa gaaaaagaag aaatagcttt 278640atttcttacc ctgatccacc ctacatacag tcagcaagca atatgggcat gcacttggca 278700tttcaaggga tggtctagta gataatggta ttttctaaaa cccctagatc ttactttcac 278760tatgcccata ctgtatctcc catgccagag ttttcttttt tcctgctgta atctagatgt 278820gttttctcca caggcatatt ttggtggtga agctcgctgc gatgctgagg ctggacgggg 278880agatgattat gatcccagag agctcatttg tggtgcctgt tctgatgttt ccagggctca 278940ggtaggcaga acattttatt agaaacaaca gtttagaaat gttaaatagt attatttttt 279000aaacaataat atggtgggaa tgtaaatcag gcttttctcc tcagtatttt ctgatatttg 279060ttcagtgttg tataattttt cacattattt catatatatt atttcctttg ggcgatattt 279120ttattttata attttgtctt ccatgaaatg atcaccatag ttaaacaatg taataaattt 279180tcttattttc ttagtttctt aaacgtgcag tgaaactttt aggttttccc ctcttcagtg 279240aaatgttcac agtacatctg tgagattata agagaggtta atttcattaa ttttcagttt 279300gaccagccat aaaacattca taaagaaatt aagtaccttg cttattaaat cagatagatt 279360atgatggaaa tggaattaga acttggatct ttgtccagac cttttggaca ggccacatat 279420ttatttgtta gttttcaaac atgtgtatca tcagtttttc aatcaatttt tagagttatg 279480taggccctca aaatatgaat taaggagctg tctatgtata tgcctatctt aagtgtgatt 279540gaaatagtct cactatgatc ttgtgtctgt ctcttctctt ttgctataga tgtgtcccaa 279600acatggcaca gactttttgg aatataaatg tcgctactgc tgttcagtgg ctgttttttt 279660ctgttttgga acaacacatt tttgtaatgc ttgtcatgat gattttcaaa gaatgactag 279720cattcctaag gaagaactac cacactgtcc tgcaggtatg cttttaatat tttaaaatca 279780cgattatgat ctatatacca tagttttatg taaacattat atgaaagctc tgtttcaagt 279840gacagaaact caattcaaac tagcctaaag aagcaggagg aattccttga tccttgtaag 279900cttatgactt tctgggtgaa ttaggaagca ggctcatcat catgagtgag aagttagctg 279960tgacggagca gatctcatgg ggagtgagaa tgaagtctac taaggattgg tgaaaagatg 280020
gctgttcagc actaaaaagt catatgaagt tcaatattac agaaccattt aaaaggattc 280080tgtgattttt tttttttttt aacgaagcct ggaaccgttg ccataaaatg aaccaagtga 280140tttaatctag aatggctggg aatcagtagt gtagtgagca tcagaaagtc aagggaatta 280200aagaaaccac tcagaataca ggagaaatta ttgtctatgg aaagcaggct gaggggaaag 280260aagtatgaaa ccaaaaagaa gctgaaaata ctggaagagg acaggaggct agtggcaacg 280320gtgaggtgga ataccaggct tcacggaagt gagagaagtg gaaggagagg tccgtgcaac 280380cataggagag aattttacat aagatatatc tgaaattagt gaaaatacat gcacaagtac 280440atacaacaca atataccgtg ttgtcaaagc tagaaaacac acactgccat ctgctgccta 280500tcagagtgca agtgatagac ctctccggaa aacagcttaa tagtgcctct caaaattacc 280560agcgtatcaa cccttcagca ctccctcttc agggatatag ctacaaatgt gaggacagta 280620aaaaagggca aataaacaag gttattaact acagctttgc tatgataata aaaatgtata 280680aacaatgcta gtgtccagta gggtagtggc taatattacg catcagttta aaaaaaaaaa 280740agagtgagga tggtgtttct gtgtgctgat ttggaaagat cttcaggaga tagcatcaga 280800tgaaaaaagt aaattaggcc aagtggtgtg actgacacct gtaatcccag cactatggga 280860gaccgaagca ggtggatcac ttgaggtcag gagtacgaga ccagcctggc caacatggtg 280920aaaccccatc tctactaaat gtacaaaaat tagccaggca tggtggcggg tacctgtaat 280980ccgagctact cacgaggctg aggccagaga atcacttgag cctgagaggc ggaggttgca 281040gtgagccaag attgcgccac tgcactccag cctgggcgac agagctgttt caaaaaaaaa 281100aagcaaattg cagaacagag tatgcagtat gctgcctttt gtaagaggga aaatgagact 281160atctagtcat atttgtttat atttacataa agaaacccct ggaaagatac tcaagaaact 281220aatactattg attaactatt gggaaaagca agaaaaagat gagggtagag actagggaaa 281280gttgcttcca attgtgttat catttttaac attttgattt taaaccatgt gaaacatata 281340ctatccattt caaaataaag ataagtaggg ttttttcaag tatgactact ttttaccatt 281400tgctcatgtc aaataaaaat tgttttactg tttctcctca aatactgttt ttttttaata 281460gaagtagcag gctttgaaag aagcaaggcc agaatgatgt gaagccaggt tatggaggat 281520aatggacaga acagagatgt gagccatgct tggagggagg gggtgatgtg ctggaggtca 281580gcaagtgaag ggagtgtgga aagggcagta ttctagatga ctctagatga cttgagcttg 281640gaggaagggc aattattgaa tgaaagttgt aggttgtggt ctggaaagaa ataggagcca 281700
agagaaaaat cagtcacacg ttgtagccat atgacctgga ggagcttggg agataaccag 281760cctccagttg agaaagattt aaggcaagca gtatcttcag gggaaatcta gattttactt 281820aaagtttcct ctatgttaaa aaacagaaat aagaatgcta aaaataagga agttaacaat 281880gaaacacttt agagaactca gtggtgggta gtagctgaga gaggagggtc aggtttcctc 281940acaggatcag ggcagccaac acaaaggaat cacaggaggg aggcggggga ggagccagag 282000ttggcagaag gaaggaatga cctcggtcct gggcattttc acacatattt gctccatttt 282060tacaatctgc gagtagatgg tattatccct atgttatcaa atggaaaatt gagggctagg 282120gaggttaatg aactggccta agaacataca ctgagattga ggtatactct cccagcctca 282180cacatccctc taactcatcc attcatttag agaactggaa tagaataaag gagatcccac 282240agggatgtga gtagggaaga tggccatata cagtttactt agaaggacag aagaagcaat 282300tctctaacca ctctcaaccc tgttaattct aatattttta gataatcagt cttcatccat 282360tcaagctgct ataacaaaat actgtaaact gggtagctta taaacagtgg aaatttattc 282420tcacagttct ggagaccagg aagtctaaga tctaggaatt agcagtcgag gtgtctggtg 282480aggacccact ctcagcctca taggtggcac tttcttgctg tcgtggaagg gcagggggcc 282540tctcctgggt ctcattgata ggaagagcac taatcccatt catggattcc accccatgac 282600ctcatcacct cccacagctc cacctcctaa caccataaca ttggtgttaa tgttgtagtc 282660tcagcagtgc accaagatat aacagtctct cattgtctga gataatgcca ggagttcttt 282720gtcctacctc caagaagatt aaggagcaca gatacaaagg tgaggttaga gcgaaagttt 282780aataagcaaa agaagaaagc tctctgccag cagagagggg ggcccaaaca ggatgctccc 282840gtgaggctgg ggcccagggt ttttatggac tggaaagggg aaggaatgtg cttagtctgt 282900gggctgtctt ggcccgcagc gtgactcagc ttggcccggg accctggccc aggaacccac 282960tggagcccac tgtgcctatg cccacaaaag gagagagccc cctgactata caaaggacaa 283020aggcatttct atcccaggtc ttgtccttta tctgattgaa ggtttttctg tctgtgcagc 283080cgtgggcatg tctttaggca caatgccctg tgctagttcc ctcatcggtg cctgcagctt 283140gacttttttt ccccaactgc tttttatgtt atatggggat gaggcactga cctgtggacc 283200tggggctctc tggggaccct tcccttgcta tctacctaag gcaaactaac tcctttcatt 283260aacacgtcag tatataaatt tggaggagca caaaaacatc cagaccatag cataatccct 283320ctatccattt ctttctaaaa tatttattga ctaccttctc gtgccaggat acttaatctt 283380
cacagcaccc catgagttag gtattgctgt gtctatttta aagatgaggt atctaagaat 283440cagaaaaaat aattttactt cagtttcata tctacaaaat gaggataatc gtttctgcct 283500cctatgactg ttaaatggtt aagtagctga tatactagaa cagtgcctca gtagtaagca 283560ctcagatgtt tgcaggtgtg ttttcactat ttttatttct caccaccatg ttggattgtg 283620aacaccttga agatgggagc catgcacttt tcatctttaa tcccaagtac ctaagaatgc 283680ccttcatata ttaggcatgc ggtaaatatt tgttgagtaa atgactcatt ttttccttga 283740aaaaacccaa gggctgcagt ttgtgtcacc accattgata tgttgtgtat ctgaactaga 283800tctgccattg agtgtcccca tcctgcgcac tctcccccac ccactggtgg ggtgggcacc 283860aatctgcagt gtgatttgga gcaggagcta ctgaactact ttctaacagg aataattaac 283920tttctagcaa acctctggca tcctcccatg ctgacccatc ctgaggtctg tgcctgcatt 283980tgtctgaggt ggcactcatt gccactctca tgcctgttag ctctaacctg aagttgactt 284040ctctgagatt agctgtcttc agtttgacac tctaatgtct tggtcttact cttgtacaga 284100acttactttt tattcctcta gatcaggcct cagtttgtgg ttgttcagta atagtagtaa 284160tagccaacat ttattgggta ttatactgtg cagtctaaga gctttataaa tgctagcctt 284220ttatccatca tggtttcctt ctcttatgtc agaaatgttt ttaaacctag aaaaggtaaa 284280gtaaatgaga cgtcattcct cattgatcag tgtaagctca ttccatccaa tctcgtgata 284340tttttctctc aagcacaatc agaatttaga tttattattt tgaaaactag ctgtagaaaa 284400ctaatgcaga gttcctattc cttcatctac tattaaatag ggactctgct ttcaagtata 284460agcctagcgt gtttttctga caaagtaagc ctcctggttt ctaaaggaac taaaaatctt 284520ttctaaatga tatgaaaccc atgacgtgaa gcacattaat ataataattt tcttttttaa 284580aatactgttt ttggtacatt tgataggcct actttgatta ttatcaacaa attcatatct 284640tccccaaagt gttttttcca cattgtattt tataacattg ttcatgtttt taacctgata 284700actttcattt tcctgctaat atccttcagg tacttttaaa aattacagga atcattggcc 284760ttcccatttt accagtgtgg gctttttcat cagtaaaaat aaaagggaaa acatggcttc 284820tttaaactta aatgttttta cttatagaaa aacattctac acaaaaatct ccctgttttc 284880tattcagaaa aataatgttc tgccagacta aattgagggg ttttgtgacc ttttttgttc 284940attactctgt tttaaaatga tatatctgat gcatttatta atagaaaaag atctgtagct 285000cgaagtgatc ctctctaggc aggatatagt gagtaaggga ccccatcact tcttccattg 285060
tacatatctg tgttgaattt tttatagtga acatttttta cttttgtgat cagaagaaaa 285120atttctaaat gttatatttg tataaccata gtcatgttat ttctttgaat aacctatcag 285180tgatttatat ttctgtgagg tttatatgtg tgtattctta aaacattatt ttcctttgcc 285240tccttcaaat ttatacaggt cccaaaggca agcagttaga aggaactgaa tgtccactcc 285300atgttgttca tccacccact ggggaagagt ttgctctggg atgtggagtg tgcagaaatg 285360cccacacttt ttagaacacg cagatccttt gtctacagag agaaaaattg ccttcatccc 285420ccaagaggat gcggtgaagt ttaaactctg ctcaggataa ggacgggacc atttttacat 285480ccatgaaaat gaaccattca cagtgcaaga aggataccaa ataccatgta cataattctt 285540gctatgaaaa gtttccccat tattttggtt tatcttcttt tgaacaaatg acatcaaact 285600tgtgaggtgt ttgcatgtgg ccattaccgt cattggcctg tgaagcattg gacatttata 285660gataattgat ataaaagaat cgccatgccc atggactaag aacgatgctg gctttcaagc 285720aaaaaagaaa aataatcatt gtttattgta tactgccttt ttgtaatcct gtacaattgc 285780atcacgggtg gggataaaaa gaggaatatt ctggtttatt tcctagactg ttatttaaaa 285840aaaaaaaaaa cattgtgtta ggacagcata taaatgtaat aagtatcaca ctgtatataa 285900acatatcaat gtttgtcctg tataagaatt actaaattac aaatgcaatt tcatttaaac 285960ttctaggtta agtttgagcc tgaaatttta atgaagtgca atactgagtg tgcctcatta 286020tcttgcagct gtaaacatat tggaatgtac atgtcaataa aaccactgta catttttata 286080cagtgataaa gtctaccact gtgggaggta ttgtttaaaa aacaaaattt gaacaccttt 286140aaggtctaaa agtccagttt tcctcagaaa gaaatttact aacacaacac attcataatt 286200ttcaaaactg ttagagaaaa taagaatagt aatgaagtgc aatgttggaa tcttaaaccc 286260taagcagaag atcataaata tatatatata tattcgtagg taaatatatt cataccaaag 286320acagaagaaa gcatttagga aattaacttc tattttaact agcagcattt ttaactttat 286380ttatttattt atgagacagg gtcttacact gtcacccagg ctggagtaca gcggcgtgat 286440ctcagctcac tgcagcctcc aactgggctc aagcgatcct ccagcctcag ccccctgagt 286500agttgggact acaggcatgc accagcacca tgcccagcta attttgtaat ttttgtagag 286560atggggtttt gccatgttgc cgaggctggt ctcaaactcc tagactcaag caatccccac 286620tgctttggcc tcccaaagtg ctaggattac aggcgtgagc cattgcaccc agcccctttt 286680tgaagttcat ataaagtaat aaaatcacta attttttatt cattttctat agacaacggt 286740
gtgtttaggt acatcttttt gacactcgag tcatgaaatt gccaaattat tgattgttga 286800ctcatcatac atcagattag caaaactttt gttgttgttg ttgtatttga gacagtctca 286860ctctgctgcc cacactggag tgcagtggca ccatcttggc tcactgcaac ctccacctcc 286920caggttcaag cgattctcct gcctcagcct cccaaggagc tgggactaca ggcgtgtgcc 286980acctcaccca gctaattttt gtatttttag tagggatggg gtttcaccag gctggtcttg 287040aactcctgac ctcaggtgat ccgctcgcct ccggcctccc aaaattccgg tattacaggc 287100gtgagccacc gcgcctggcc aagactagca aaagttttaa tcacattttg ttctaaattc 287160caactgtggg aaatatagaa cttaatttgt caatatagaa ttactgacaa atttaatgac 287220atttgtgtta agcatttatg agaaatttaa aacaatcttt gccttgaagg aacttaaaaa 287280ataaagatca gatctgagca caggtaacta ttttacagaa tgttctaata agtttcataa 287340aaatatccaa gccaaatgct tcaggattta ggaaaaatca ttattgtttt gtcgttggga 287400gtgtagctaa gaggccattt aagctgcctt ttgaagaata agtagcattt caaggagcag 287460agatgtggag aaaggcatga gaaataccta agtaggaaaa tctagaatat gatcaaagaa 287520cagtaagtat gagtgttcaa gcttaggatt gactgaaaat gtttccagaa aacacaaagt 287580acgtagagag actaaagctg ttacaagaaa actggagggg agaatgcagt gaggacactg 287640tcagtatatt tgacttgaca ctgtcagtat atttgactta aattcacagg ccacaggaat 287700gaaactaacc ttgagaatgc cacacagata agagacttta tggacactta atagctgcga 287760agaataccat cttgcgtcag ttaactttgc tggcaaactg gtcagctcag tttgtaaatc 287820gtagaggaaa agaaacaaac tattgagatg gcttggctaa aaaagccatc tcaagttatg 287880aaagggataa aatcaacaaa gctgtgtagt gaatactcac aatgacttca acttatcttc 287940atttatgagg aagtcacttt tagtcaggat ctctagcacc tcaaaaccag agaacataaa 288000catcttagca ataaattatg acaaagcaca ggaaggagtg gctttggcag gcatttttag 288060ctcaagcatc ctacacacaa ggaccatcct tcaaacactg catgtaacat ccaaggcagg 288120agacagaaag cagaatcaca gtggtagatg tcagtgaatc ttcctggaaa gaaattgttt 288180ttccgttata ttttcaagcc taggaaaagc catttttctt agtattatgg ctagactgtt 288240taggcctaaa atctccctat tttatcaata agcttatttg gttttggttt ttggtttttt 288300gagttttttg ggtttttttt ttttttttta agaaagggtc tcactctgtc gccccagctg 288360gagtgcagtg gcgttatctc ggctcgcagc aacctctgcc ttttgggctc aagtgatcct 288420
cccacctcag ccccaagtag ctgggagtac aggcacgagc caccatgccc agctaatttt 288480tgtaattgaa acagggtttc accatgttgg ccaggctggt ctcaaactcc tgagctgaag 288540cgatctacct gcctcagcta ggattacagg tgtgagccac cgcacccaac ctcaataagc 288600ttatttgata aaatatatgc aatgctccct ttattcactt ttcattcaga atgtttagta 288660atttgtattg tttttcagat tttcagccca atatatctcc ctgcccactg tgtcactgta 288720ttctacctat acatcatcac gtgtttctgc tattggctgt atgatggaac actgcggctc 288780attttcctga aaactgccga tagtgcatag agtgctggga tggaaaccag aagctttgaa 288840ttcaagcctt ggttctgcct tgtttttgct tgggtggcct tgagtcagcc acataccttt 288900taaaatctca atttattaga aattattcca aatcaaaatc aaatgagaag gtatatacaa 288960aagtgcttta tcccacaata aactattcaa gagagagcaa aggagaggac atttactcaa 289020cacctcctaa aaggcagcca gtgaaattag gcattttatt taatcctcct ggcaactctg 289080agagtaaagc attattaatc ccattttggc tgtttaaaga aattatttgc actagattcc 289140agctgtagtt tagcttcaga aaaaaaaatc ctgagatgtg aattcacagc tttctgggtt 289200taaagcccaa gctctatcac atcatgctat tattgttaca ttactgctag ttctatgaaa 289260agaaatacta atttatgaaa tacatcttat ccaaaatggt tgggaccaga agtgtttctg 289320atttcgggtt ttttcaggtt tgggaatatt tgcattacca gttgagcatc ccaaatctga 289380aatccaaaat gctccagtga gcattttatt tgggtatcag gcatgtcagt actcaaagtt 289440ttaaatttca ggacacttat cgtatggcca aggttgcaca ggggacaaga cagcatatgc 289500ctccaagagt gttcagtgtc gtcaggaggt acctccaccc agatttccct tcaggggagg 289560ccccacctca aggagtccag ttagctgaca gcctccagct ttaatcaccc ccacatatga 289620cccaatgtca cactcctcca gggcagcccc agcctgtgat agagttcaga tggggttctc 289680gggcctggcc atttctgccc aagcggcact cctgcgtgcg cggtcttgcg taagaactca 289740ggccgactga gactgtctta cagctgccct gcagtcggag gcttttccta cccaatcttt 289800ccttctctct tctttcctag atgccagacc tgcatcacag tctaatggct ctccctgccc 289860actcctgctc cctcatcact ttatctttca tagggattac ccccaacaga ttttctgcac 289920ttctaactta atcttggcat ctgcttccta gaggacctgc ctgacacagg agggtaatac 289980attttaaatg acttacttat ggcttcattt ctcaaatgtc ttagggtggc ttagaaaaaa 290040<210>4
<211>739<212>DNA<213>褐家鼠(Rattus norvegicus)<400>4gcttgccaaa cgattcaggt gccaacaata ctaaattcac tacagagaag tgtacaggca 60gtgttggttg gaaagattca agtccaggac tggtttagca atggcattaa gaaagcagct120ttaatgcaca agtggccatt aaaggaaata tctgttgatg aagatgacca gtgtctgctt180cagaatgatg gattttttct ttatttgtta tgcaaggatg gattatacaa aataggctct240ggatacagtg gaacagttag gggtcatata tataattcta catctcgtat cagaaacaga300aaagaaaaga agtcttggtt aggatatgcc cagggttact tgttatatcg ggatgtgaat360aaccacagta tgacagccat aagaataagc cccgaaacgc tggagcaaga cggcactgta420atgttaccag cttgcagatg ttgcttgaca cattcctgaa gctgctgtct tataatgtca480tacacgggta aagagcggac acgggaagtt gtcagtgtga cgccgaagga agacccagcc540ctcagcccca gccctcaagc ctgaagaagc ctgctgtcat ttcatcactt ctttgacacg600gactacagtt tcttcaataa aatcatttgc tttttccctg acaagggata ctacttctct660gtagcttttc atcaagaaga gaagtagtaa actgtcagat ttataggtgg ttactgaata720ccatgtttaa aatgaacat 739<210>5<211>473<212>DNA<213>褐家鼠<400>5gcagcttcag agtgacagag gaactgtctc aacatcttca agaccagtgt ctacatcagc 60aaagtcagag ctgccctcca agaacagcag atcagttaaa cctgatgggc gtgtgagccg120gactactgct gaccagaaga agccacgggg cacagaaggc ttatctgcta gtgaatccct180catgttaaaa tctgatgctg caaagttgag gtcagactcc catagtaggt cactgtcccc240taaccataac actttgcaga cactgaagtc tgatggaagg gtatcttcta gcttcagggc300tgaatcccca ggaccaggct ctaggtcatc ctctcctaag cccaagactc tgccgactcc360caggtctagc ccatctggtg ctagctctcc acgctcctcc tcaccgcagg ataaaaatct420acctcagaaa agcacagctc ctgctaagac aaaacttgac ccacctcggg agc 473<210>6
<211>708<212>DNA<213>褐家鼠<220>
<221>misc_feature<222>(615)..(615)<223>n是a，c，g或t<400>6tttttttttt ttttttttcc tttttcgttt ttttattctc tttctcacat tctttctttt 60taaggactgc acaggaacct ggacttggaa aaatcatatt ctgggaagca gctttgattg120tagccaaaga gatgtcctcc cagaaggcca ctaagtgttg taatgttaag gggagcggag180acctagactt cactgagtga tgcatggaca tttcaaaagt ggcttccgat tttccgtctt240cacacttctc atgtagaggt ggctccttaa gcatagacag gaccttattt ttgttgatac300ttcccatctt ggatatgtct ggtccgtgag gtgaaatgtt gaacatattt agggcagatg360atatttccaa cgaatgtcta ttttttaact tgatttcctt ttcctctgtg ggtggctggc420tattcaaact acttcttatg ggagcatgtt ctttggaaag ttcaggatta aacttcagga480aggaagagca ggccattgca tcatgtacta tgccttcatg ccacagaaaa gaggcaaaga540cagctcgggc acactcagcc acggacgggg acatggcttg cttggctggc tctaaagatt600tggctccatc tcctnttgaa agaaagttag acttttcttt tttgggcctg gtgtgtctat660tagcacattt ccgacttatt ttggggtgat gctctcattt cctgttca 708<210>7<211>529<212>DNA<213>褐家鼠<400>7taagcttgct ctgtccctgc cccgtacatc tcagtaactc ctgatgcaag tcccaatgtc 60tttgaagagc cggagagcaa tatgaagtcg atgccaccaa gtttggaaac gagcccgata120actgacaccg acctggctaa gagaactgtc ttccagaggt catactcagt tgtcgcttcg180gaatatgata aacaacactc cattttacct gcacgagtta aagccatccc tagaaggaga240gtgaacagtg gagacacgga agttgggtct tctctcttgc gacatccgtc accggagctt300tcccggctta tatcagccca cagctctctc tccaaaggag agcgaaactt ccagtggcca360gtcttagctt tcgtcataca gcatcatgat ttagaagggc tggaaatcgc aatgaagcag420
gccttaagga agtcggcttg ccgtgtgttt gctatggagg cattcaactg gcttctctgt480aatgtcatcc aaacaacgtc tctgcatgac attctctggc actttgtgg529<210>8<211>667<212>DNA<213>褐家鼠<400>8acgaagggct gcataagagt gaagcgatca cgacgccggg cgtcaggttt tacaatgatc 60cagccggcta tgccatgaac agatacgcat attatgtttg ctacaaatgc agaaaggcat120attttggtgg tgaagctcgc tgtgatgctg aggctggaca aggagacgac tacgacccca180gagagctcat ctgtggagcc tgttctgatg tgtctagggc tcagatgtgt cccaaacatg240gaacagactt tctagaatac aaatgtcgct actgctgttc agtggctgtc ttcttctgtt300ttggaacaac acatttctgc aatgcttgtc atgatgattt tcaaagaatg accagcattc360ctaaggaaga gctcccacac tgtcctgcag gtcccaaagg caaacagcta gaaggaactg420aatgtccact ccatgttgtt catccgccca cgggggaaga gtttgctctt ggttgtggag480tgtgcagaaa tgctcacacg ttttagaact ttcagatcct ttgtctacaa agaggatagt540tgccttcatc ccctgggagg atgcagtgaa actttaaact ctgctcaagg ataaggaacg600gggaccattt ttacattctg aaaacgaacc attttccagt gccaggaagt gatgccccaa660atacctg 667<210>9<211>652<212>DNA<213>褐家鼠<220>
<221>misc_feature<222>(605)..(605)<223>n是a，c，g或t<400>9tttttttttt ttttttttga cgctctcact gtgtagagca cagtggttta ctgacgtaca 60ttccaatatg ctgacagctg caaggtagtg aggcacaccc agtattgcac tgcattagag120cttcaggctc aaacgtaacc tacaggttta aatgagactg ctgtgtcatt tagtaattct180catacaggac aaacactgat agctttatat acagtctgat acttattaca attataggcc240
ttactaacac aatttttttt tttaaataac agtctaggaa agaaaccaga atattcctct300ttttataccc acccgtgatg caattgtaca ggattacaaa aaggcagtat acaataaaca360gtgattattt ttcttttttt gcttgaaagc cagcatcatt cttagtccat gagtatggag420atccttttat atcaattatc tataaatgtc caatgctcca caggccagtg acggtaatgg480ccacatgcaa acacctcaca agtttgatgt cttggttcaa agatgataaa ccaaaacaat540ggggaaacgt tcgtagcaag aattatgtac acagtatttg gcatcactcc tgcactggaa600atggntcgtt ttcagaatgt aaaatggtcc cgttcttatc ctgagcagag tt652<210>10<211>18<212>DNA<213>人工序列<400>10gaagggtggg gccaaaag 18<210>11<211>20<212>DNA<213>人工序列<400>11ggatgcaggg atgatgttct 20<210>12<211>24<212>DNA<213>人工序列<400>12ggtggtgaag ctcgctgtga tgct24<210>13<211>24<212>DNA<213>人工序列<400>13cgtgtgagca tttctgcaca ctcc24<210>14<211>18<212>DNA<213>人工序列
<400>14gactggttta gcaatggc 18<210>15<211>18<212>DNA<213>人工序列<400>15gccattgcta aaccagtc 18<210>16<211>18<212>DNA<213>人工序列<400>16gcaattgcta aatcagta 18<210>17<211>1010<212>PRT<213>人<400>17Asn Asn His Ser Met Thr Ala Ile Arg Ile Ser Pro Glu Thr Leu Glu1 5 10 15Gln Asp Gly Thr Val Met Leu Pro Asp Cys His Thr Glu Gly Gln Asn20 25 30Ile Leu Phe Thr Asp Gly Glu Tyr Ile Asn Gln Ile Ala Ala Ser Arg35 40 45Asp Asp Gly Phe Val Val Arg Ile Phe Ala Thr Ser Thr Glu Pro Val50 55 60Leu Gln Gln Glu Leu Gln Leu Lys Leu Ala Arg Lys Cys Leu His Ala65 70 75 80Cys Arg Ile Ser Leu Phe Asp Leu Glu Lys Asp Leu His Ile Ile Ser85 90 95Thr Gly Phe Asp Glu Glu Ser Ala Ile Leu Gly Ala Gly Arg Glu Phe100 105 110
Ala Leu Met Lys Thr Ala Asn Gly Lys Ile Tyr Tyr Thr Gly Lys Tyr115 120 125Gln Ser Leu Gly Ile Lys Gln Gly Gly Pro Ser Ala Gly Lys Trp Val130 135 140Glu Leu Pro Ile Thr Lys Ser Pro Lys Ile Val His Phe Ser Val Gly145 150 155 160His Asp Gly Ser His Ala Leu Leu Val Ala Glu Asp Gly Ser Ile Phe165 170 175Phe Thr Gly Ser Ala Ser Lys Gly Glu Asp Gly Glu Ser Ile Lys Ser180 185 190Arg Arg Gln Ser Lys Pro Tyr Lys Pro Lys Lys Ile Ile Lys Met Glu195 200 205Gly Lys Ile Val Val Tyr Thr Ala Cys Asn Asn Gly Ser Ser Ser Val210 215 220Ile Ser Lys Asp Gly Glu Leu Tyr Met Phe Gly Lys Asp Ala Ile Tyr225 230 235 240Ser Asp Ser Ser Ser Leu Val Thr Asp Leu Lys Gly His Phe Val Thr245 250 255Gln Val Ala Met Gly Lys Ala His Thr Cys Val Leu Met Lys Asn Gly260 265 270Glu Val Trp Thr Phe Gly Val Asn Asn Lys Gly Gln Cys Gly Arg Asp275 280 285Thr Gly Ala Met Asn Gln Gly Gly Lys Gly Phe Gly Val Glu Asn Met290 295 300Ala Thr Ala Met Asp Glu Asp Leu Glu Glu Glu Leu Asp Glu Lys Asp305 310 315 320Glu Lys Ser Met Met Cys Pro Pro Gly Met His Lys Trp Lys Leu Glu325 330 335
Gln Cys Met Val Cys Thr Val Cys Gly Asp Cys Thr Gly Tyr Gly Ala340 345 350Ser Cys Val Ser Ser Gly Arg Pro Asp Arg Val Pro Gly Gly Ile Cys355 360 365Gly Cys Gly Ser Gly Glu Ser Gly Cys Ala Val Cys Gly Cys Cys Lys370 375 380Ala Cys Ala Arg Glu Leu Asp Gly Gln Glu Ala Arg Gln Arg Gly Ile385 390 395 400Leu Asp Ala Val Lys Glu Met Ile Pro Leu Asp Leu Leu Leu Ala Val405 410 415Pro Val Pro Gly Val Asn Ile Glu Glu His Leu Gln Leu Arg Gln Glu420 425 430Glu Lys Arg Gln Arg Val Ile Arg Arg His Arg Leu Glu Glu Gly Arg435 440 445Gly Pro Leu Val Phe Ala Gly Pro Ile Phe Met Asn His Arg Glu Gln450 455 460Ala Leu Ala Arg Leu Arg Ser His Pro Ala His Val Lys His Lys Arg465 470 475 480Asp Lys His Lys Asp Gly Ser Gly Glu Arg Gly Glu Lys Asp Ala Ser485 490 495Lys Ile Thr Thr Tyr Pro Pro Gly Ser Val Arg Phe Asp Cys Glu Leu500 505 510Arg Ala Val Gln Val Ser Cys Gly Phe His His Ser Val Val Leu Met515 520 525Glu Asn Gly Asp Val Tyr Thr Phe Gly Tyr Gly Gln His Gly Gln Leu530 535 540Gly His Gly Asp Val Asn Ser Arg Gly Cys Pro Thr Leu Val Gln Ala545 550 555 560
Leu Pro Gly Pro Ser Thr Gln Val Thr Ala Gly Ser Asn His Thr Ala565 570 575Val Leu Leu Met Asp Gly Gln Val Phe Thr Phe Gly Ser Phe Ser Lys580 585 590Gly Gln Leu Gly Arg Pro Ile Leu Asp Val Pro Tyr Trp Asn Ala Lys595 600 605Pro Ala Pro Met Pro Asn Ile Gly Ser Lys Tyr Gly Arg Lys Ala Thr610 615 620Trp Ile Gly Ala Ser Gly Asp Gln Thr Phe Leu Arg Ile Asp Glu Ala625 630 635 640Leu Ile Asn Ser His Val Leu Ala Thr Ser Glu Ile Phe Ala Ser Lys645 650 655His Ile Ile Gly Leu Val Pro Ala Ser Ile Ser Glu Pro Pro Pro Phe660 665 670Lys Cys Leu Leu Ile Asn Lys Val Asp Gly Ser Cys Lys Thr Phe Asn675 680 685Asp Ser Glu Gln Glu Asp Leu Gln Gly Phe Gly Val Cys Leu Asp Pro690 695 700Val Tyr Asp Val Ile Trp Arg Phe Arg Pro Asn Thr Arg Glu Leu Trp705 710 715 720Cys Tyr Asn Ala Val Val Ala Asp Ala Arg Leu Pro Ser Ala Ala Asp725 730 735Met Gln Ser Arg Cys Ser Ile Leu Ser Pro Glu Leu Ala Leu Pro Thr740 745 750Gly Ser Arg Ala Leu Thr Thr Arg Ser His Ala Ala Leu His Ile Leu755 760 765Gly Cys Leu Asp Thr Leu Ala Ala Met Gln Asp Leu Lys Met Gly Val770 775 780
Ala Ser Thr Glu Glu Glu Thr Gln Ala Val Met Lys Val Tyr Ser Lys785 790 795 800Glu Asp Tyr Ser Val Val Asn Arg Phe Glu Ser His Gly Gly Gly Trp805 810 815Gly Tyr Ser Ala His Ser Val Glu Ala Ile Arg Phe Ser Ala Asp Thr820 825 830Asp Ile Leu Leu Gly Gly Leu Gly Leu Phe Gly Gly Arg Gly Glu Tyr835 840 845Thr Ala Lys Ile Lys Leu Phe Glu Leu Gly Pro Asp Gly Gly Asp His850 855 860Glu Thr Asp Gly Asp Leu Leu Ala Glu Thr Asp Val Leu Ala Tyr Asp865 870 875 880Cys Ala Ala Arg Glu Lys Tyr Ala Met Met Phe Asp Glu Pro Val Leu885 890 895Leu Gln Ala Gly Trp Trp Tyr Val Ala Trp Ala Arg Val Ser Gly Pro900 905 910Ser Ser Asp Cys Gly Ser His Gly Gln Ala Ser Ile Thr Thr Asp Asp915 920 925Gly Val Val Phe Gln Phe Lys Ser Ser Lys Lys Ser Asn Asn Gly Thr930 935 940Asp Val Asn Ala Gly Gln Ile Pro Gln Leu Leu Tyr Arg Leu Pro Thr945 950 955 960Ser Asp Gly Ser Ala Ser Lys Gly Lys Gln Gln Thr Ser Glu Pro Val965 970 975His Ile Leu Lys Arg Ser Phe Ala Arg Thr Val Ser Val Glu Cys Phe980 985 990Glu Ser Leu Leu Ser Ile Leu His Trp Ser Trp Thr Thr Leu Val Leu995 1000 1005
Gly Val1010<210>18<211>617<212>PRT<213>人<400>18Ile Phe Ala Thr Ser Thr Glu Pro Val Leu Gln Gln Glu Leu Gln Leu1 5 10 15Lys Leu Ala Arg Lys Cys Leu His Ala Cys Arg Ile Ser Leu Phe Asp20 25 30Leu Glu Lys Asp Leu His Ile Ile Ser Thr Gly Phe Asp Glu Glu Ser35 40 45Ala Ile Leu Gly Ala Gly Arg Glu Phe Ala Leu Met Lys Thr Ala Asn50 55 60Gly Lys Ile Tyr Tyr Thr Gly Lys Tyr Gln Ser Leu Gly Ile Lys Gln65 70 75 80Gly Gly Pro Ser Ala Gly Lys Trp Val Glu Leu Pro Ile Thr Lys Ser85 90 95Pro Lys Ile Val His Phe Ser Val Gly His Asp Gly Ser His Ala Leu100 105 110Leu Val Ala Glu Asp Gly Ser Ile Phe Phe Thr Gly Ser Ala Ser Lys115 120 125Gly Glu Asp Gly Glu Ser Ile Lys Ser Arg Arg Gln Ser Lys Pro Tyr130 135 140Lys Pro Lys Lys Ile Ile Lys Met Glu Gly Lys Ile Val Val Tyr Thr145 150 155 160Ala Cys Asn Asn Gly Ser Ser Ser Val Ile Ser Lys Asp Gly Glu Leu165 170 175
Tyr Met Phe Gly Lys Asp Ala Ile Tyr Ser Asp Ser Ser Ser Leu Val180 185 190Thr Asp Leu Lys Gly His Phe Val Thr Gln Val Ala Met Gly Lys Ala195 200 205His Thr Cys Val Leu Met Lys Asn Gly Glu Val Trp Thr Phe Gly Val210 215 220Asn Asn Lys Gly Gln Cys Gly Arg Asp Thr Gly Ala Met Asn Gln Gly225 230 235 240Gly Lys Gly Phe Gly Val Glu Asn Met Ala Thr Ala Met Asp Glu Asp245 250 255Leu Glu Glu Glu Leu Asp Glu Lys Asp Glu Lys Ser Met Met Cys Pro260 265 270Pro Gly Met His Lys Trp Lys Leu Glu Gln Cys Met Val Cys Thr Val275 280 285Cys Gly Asp Cys Thr Gly Tyr Gly Ala Ser Cys Val Ser Ser Gly Arg290 295 300Pro Asp Arg Val Pro Gly Gly Ile Cys Gly Cys Gly Ser Gly Glu Ser305 310 315 320Gly Cys Ala Val Cys Gly Cys Cys Lys Ala Cys Ala Arg Glu Leu Asp325 330 335Gly Gln Glu Ala Arg Gln Arg Gly Ile Leu Asp Ala Val Lys Glu Met340 345 350Ile Pro Leu Asp Leu Leu Leu Ala Val Pro Val Pro Gly Val Asn Ile355 360 365Glu Glu His Leu Gln Leu Arg Gln Glu Glu Lys Arg Gln Arg Val Ile370 375 380Arg Arg His Arg Leu Glu Glu Gly Arg Gly Pro Leu Val Phe Ala Gly385 390 395 400
Pro Ile Phe Met Asn His Arg Glu Gln Ala Leu Ala Arg Leu Arg Ser405 410 415His Pro Ala His Val Lys His Lys Arg Asp Lys His Lys Asp Gly Ser420 425 430Gly Glu Arg Gly Glu Lys Asp Ala Ser Lys Ile Thr Thr Tyr Pro Pro435 440 445Gly Ser Val Arg Phe Asp Cys Glu Leu Arg Ala Val Gln Val Ser Cys450 455 460Gly Phe His His Ser Val Val Leu Met Glu Asn Gly Asp Val Tyr Thr465 470 475 480Phe Gly Tyr Gly Gln His Gly Gln Leu Gly His Gly Asp Val Asn Ser485 490 495Arg Gly Cys Pro Thr Leu Val Gln Ala Leu Pro Gly Pro Ser Thr Gln500 505 510Val Thr Ala Gly Ser Asn His Thr Ala Val Leu Leu Met Asp Gly Gln515 520 525Val Phe Thr Phe Gly Ser Phe Ser Lys Gly Gln Leu Gly Arg Pro Ile530 535 540Leu Asp Val Pro Tyr Trp Asn Ala Lys Pro Ala Pro Met Pro Asn Ile545 550 555 560Gly Ser Lys Tyr Gly Arg Lys Ala Thr Trp Ile Gly Ala Ser Gly Asp565 570 575Gln Thr Phe Leu Arg Ile Asp Glu Ala Leu Ile Asn Ser His Val Leu580 585 590Ala Thr Ser Glu Ile Phe Ala Ser Lys His Ile Ile Gly Leu Val Pro595 600 605Ala Ser Ile Ser Glu Pro Pro Pro Phe610 615
<210>19<211>567<212>PRT<213>人<400>19Gly Arg Glu Phe Ala Leu Met Lys Thr Ala Asn Gly Lys Ile Tyr Tyr1 5 10 15Thr Gly Lys Tyr Gln Ser Leu Gly Ile Lys Gln Gly Gly Pro Ser Ala20 25 30Gly Lys Trp Val Glu Leu Pro Ile Thr Lys Ser Pro Lys Ile Val His35 40 45Phe Ser Val Gly His Asp Gly Ser His Ala Leu Leu Val Ala Glu Asp50 55 60Gly Ser Ile Phe Phe Thr Gly Ser Ala Ser Lys Gly Glu Asp Gly Glu65 70 75 80Ser Ile Lys Ser Arg Arg Gln Ser Lys Pro Tyr Lys Pro Lys Lys Ile85 90 95Ile Lys Met Glu Gly Lys Ile Val Val Tyr Thr Ala Cys Asn Asn Gly100 105 110Ser Ser Ser Val Ile Ser Lys Asp Gly Glu Leu Tyr Met Phe Gly Lys115 120 125Asp Ala Ile Tyr Ser Asp Ser Ser Ser Leu Val Thr Asp Leu Lys Gly130 135 140His Phe Val Thr Gln Val Ala Met Gly Lys Ala His Thr Cys Val Leu145 150 155 160Met Lys Asn Gly Glu Val Trp Thr Phe Gly Val Asn Asn Lys Gly Gln165 170 175Cys Gly Arg Asp Thr Gly Ala Met Asn Gln Gly Gly Lys Gly Phe Gly180 185 190Val Glu Asn Met Ala Thr Ala Met Asp Glu Asp Leu Glu Glu Glu Leu
195 200 205Asp Glu Lys Asp Glu Lys Ser Met Met Cys Pro Pro Gly Met His Lys210 215 220Trp Lys Leu Glu Gln Cys Met Val Cys Thr Val Cys Gly Asp Cys Thr225 230 235 240Gly Tyr Gly Ala Ser Cys Val Ser Ser Gly Arg Pro Asp Arg Val Pro245 250 255Gly Gly Ile Cys Gly Cys Gly Ser Gly Glu Ser Gly Cys Ala Val Cys260 265 270Gly Cys Cys Lys Ala Cys Ala Arg Glu Leu Asp Gly Gln Glu Ala Arg275 280 285Gln Arg Gly Ile Leu Asp Ala Val Lys Glu Met Ile Pro Leu Asp Leu290 295 300Leu Leu Ala Val Pro Val Pro Gly Val Asn Ile Glu Glu His Leu Gln305 310 315 320Leu Arg Gln Glu Glu Lys Arg Gln Arg Val Ile Arg Arg His Arg Leu325 330 335Glu Glu Gly Arg Gly Pro Leu Val Phe Ala Gly Pro Ile Phe Met Asn340 345 350His Arg Glu Gln Ala Leu Ala Arg Leu Arg Ser His Pro Ala His Val355 360 365Lys His Lys Arg Asp Lys His Lys Asp Gly Ser Gly Glu Arg Gly Glu370 375 380Lys Asp Ala Ser Lys Ile Thr Thr Tyr Pro Pro Gly Ser Val Arg Phe385 390 395 400Asp Cys Glu Leu Arg Ala Val Gln Val Ser Cys Gly Phe His His Ser405 410 415Val Val Leu Met Glu Asn Gly Asp Val Tyr Thr Phe Gly Tyr Gly Gln
420 425 430His Gly Gln Leu Gly His Gly Asp Val Asn Ser Arg Gly Cys Pro Thr435 440 445Leu Val Gln Ala Leu Pro Gly Pro Ser Thr Gln Val Thr Ala Gly Ser450 455 460Asn His Thr Ala Val Leu Leu Met Asp Gly Gln Val Phe Thr Phe Gly465 470 475 480Ser Phe Ser Lys Gly Gln Leu Gly Arg Pro Ile Leu Asp Val Pro Tyr485 490 495Trp Asn Ala Lys Pro Ala Pro Met Pro Asn Ile Gly Ser Lys Tyr Gly500 505 510Arg Lys Ala Thr Trp Ile Gly Ala Ser Gly Asp Gln Thr Phe Leu Arg515 520 525Ile Asp Glu Ala Leu Ile Asn Ser His Val Leu Ala Thr Ser Glu Ile530 535 540Phe Ala Ser Lys His Ile Ile Gly Leu Val Pro Ala Ser Ile Ser Glu545 550 555 560Pro Pro Pro Phe Lys Cys Leu565<210>20<211>301<212>PRT<213>人<400>20Ala Pro Met Pro Asn Ile Gly Ser Lys Tyr Gly Arg Lys Ala Thr Trp1 5 10 15Ile Gly Ala Ser Gly Asp Gln Thr Phe Leu Arg Ile Asp Glu Ala Leu20 25 30Ile Asn Ser His Val Leu Ala Thr Ser Glu Ile Phe Ala Ser Lys His35 40 45
Ile Ile Gly Leu Val Pro Ala Ser Ile Ser Glu Pro Pro Pro Phe Lys50 55 60Cys Leu Leu Ile Asn Lys Val Asp Gly Ser Cys Lys Thr Phe Asn Asp65 70 75 80Ser Glu Gln Glu Asp Leu Gln Gly Phe Gly Val Cys Leu Asp Pro Val85 90 95Tyr Asp Val Ile Trp Arg Phe Arg Pro Asn Thr Arg Glu Leu Trp Cys100 105 110Tyr Asn Ala Val Val Ala Asp Ala Arg Leu Pro Ser Ala Ala Asp Met115 120 125Gln Ser Arg Cys Ser Ile Leu Ser Pro Glu Leu Ala Leu Pro Thr Gly130 135 140Ser Arg Ala Leu Thr Thr Arg Ser His Ala Ala Leu His Ile Leu Gly145 150 155 160Cys Leu Asp Thr Leu Ala Ala Met Gln Asp Leu Lys Met Gly Val Ala165 170 175Ser Thr Glu Glu Glu Thr Gln Ala Val Met Lys Val Tyr Ser Lys Glu180 185 190Asp Tyr Ser Val Val Asn Arg Phe Glu Ser His Gly Gly Gly Trp Gly195 200 205Tyr Ser Ala His Ser Val Glu Ala Ile Arg Phe Ser Ala Asp Thr Asp210 215 220Ile Leu Leu Gly Gly Leu Gly Leu Phe Gly Gly Arg Gly Glu Tyr Thr225 230 235 240Ala Lys Ile Lys Leu Phe Glu Leu Gly Pro Asp Gly Gly Asp His Glu245 250 255Thr Asp Gly Asp Leu Leu Ala Glu Thr Asp Val Leu Ala Tyr Asp Cys260 265 270
Ala Ala Arg Glu Lys Tyr Ala Met Met Phe Asp Glu Pro Val Leu Leu275 280 285Gln Ala Gly Trp Trp Tyr Val Ala Trp Ala Arg Val Ser290 295 300<210>21<211>101<212>PRT<213>人<400>21Ala Pro Met Pro Asn Ile Gly Ser Lys Tyr Gly Arg Lys Ala Thr Trp1 5 10 15Ile Gly Ala Ser Gly Asp Gln Thr Phe Leu Arg Ile Asp Glu Ala Leu20 25 30Ile Asn Ser His Val Leu Ala Thr Ser Glu Ile Phe Ala Ser Lys His35 40 45Ile Ile Gly Leu Val Pro Ala Ser Ile Ser Glu Pro Pro Pro Phe Lys50 55 60Cys Leu Leu Ile Asn Lys Val Asp Gly Ser Cys Lys Thr Phe Asn Asp65 70 75 80Ser Glu Gln Glu Asp Leu Gln Gly Phe Gly Val Cys Leu Asp Pro Val85 90 95Tyr Asp Val Ile Trp100<210>22<211>204<212>PRT<213>人<400>22Asp Glu Ala Leu Ile Asn Ser His Val Leu Ala Thr Ser Glu Ile Phe1 5 10 15
Ala Ser Lys His Ile Ile Gly Leu Val Pro Ala Ser Ile Ser Glu Pro20 25 30Pro Pro Phe Lys Cys Leu Leu Ile Asn Lys Val Asp Gly Ser Cys Lys35 40 45Thr Phe Asn Asp Ser Glu Gln Glu Asp Leu Gln Gly Phe Gly Val Cys50 55 60Leu Asp Pro Val Tyr Asp Val Ile Trp Arg Phe Arg Pro Asn Thr Arg65 70 75 80Glu Leu Trp Cys Tyr Asn Ala Val Val Ala Asp Ala Arg Leu Pro Ser85 90 95Ala Ala Asp Met Gln Ser Arg Cys Ser Ile Leu Ser Pro Glu Leu Ala100 105 110Leu Pro Thr Gly Ser Arg Ala Leu Thr Thr Arg Ser His Ala Ala Leu115 120 125His Ile Leu Gly Cys Leu Asp Thr Leu Ala Ala Met Gln Asp Leu Lys130 135 140Met Gly Val Ala Ser Thr Glu Glu Glu Thr Gln Ala Val Met Lys Val145 150 155 160Tyr Ser Lys Glu Asp Tyr Ser Val Val Asn Arg Phe Glu Ser His Gly165 170 175Gly Gly Trp Gly Tyr Ser Ala His Ser Val Glu Ala Ile Arg Phe Ser180 185 190Ala Asp Thr Asp Ile Leu Leu Gly Gly Leu Gly Leu195 200<210>23<21l>38<212>PRT<213>人<400>23Asp Glu Ala Leu Ile Asn Ser His Val Leu Ala Thr Ser Glu Ile Phe
1 5 10 15Ala Ser Lys His Ile Ile Gly Leu Val Pro Ala Ser Ile Ser Glu Pro20 25 30Pro Pro Phe Lys Cys Leu35<210>24<211>3030<212>DNA<213>人<400>24aataaccaca gcatgacagc cataaggata agccctgaaa cactggagca agatggtact 60gtgatgttac cagattgcca cactgaaggt caaaatattt tattcactga tggagaatat120attaatcaga tagctgcttc aagagatgat ggctttgttg tcagaatatt tgccacaagc180actgaacctg ttctacagca agaattgcaa cttaaactgg ctagaaaatg cttacatgcc240tgtcgtatct cattattcga tctggaaaag gacttgcata ttataagtac aggatttgat300gaggagtcag caattcttgg tgcaggacga gagtttgcgc taatgaaaac agcaaatgga360aagatatatt acactggcaa ataccagagt cttggaatca aacaaggtgg tccttcagca420ggaaaatggg ttgagctacc aattacaaaa tctccaaaga tagtacactt ctcagttgga480cacgatggct ctcacgccct tttagttgca gaagatggga gcatattctt tacaggatct540gctagtaaag gagaagatgg agaatcaatt aagagcagac ggcaatccaa accttataaa600cctaaaaaga taattaagat ggaaggaaag attgtggtat atacagcctg caataatgga660agtagttctg ttatttctaa agatggagaa ctctacatgt ttggaaaaga tgccatttac720tctgatagtt caagtttggt aactgatttg aagggccatt ttgtaactca ggtagctatg780ggcaaagctc acacttgtgt tttaatgaag aatggagagg tgtggacatt tggtgtaaat840aataaaggac agtgtggacg agatactggt gccatgaacc aaggtgggaa agggtttgga900gttgaaaata tggcaacagc aatggatgaa gacctggaag aagaactaga tgaaaaagat960gagaagtcta tgatgtgccc tccaggcatg cacaaatgga agctggagca gtgcatggtt 1020tgcactgtct gtggagactg tacaggttat ggagccagct gtgtcagtag tggacggcca 1080gacagagtcc ccggagggat ctgtggttgt ggttccggag aatctggttg tgctgtgtgt 1140ggatgttgca aggcctgtgc aagagagtta gatggtcaag aggcaagaca aagaggaatt 1200
cttgatgcag tgaaagaaat gataccttta gatcttcttt tagctgtccc agtgcccggg1260gttaacattg aagaacacct tcagttacga caagaagaaa aacggcaacg tgtaatcaga1320aggcacagat tagaggaagg aagaggcccc cttgtatttg ctggtcctat ttttatgaac1380catcgagaac aggctctagc cagactcaga tcccatccag cacacgtaaa gcataaacgg1440gacaagcaca aagatggaag tggagaaaga ggcgaaaagg atgcaagcaa aatcacaaca1500taccctccag gctctgtgcg atttgactgt gagctccggg cagtccaagt cagctgtgga1560tttcaccatt cagtggtttt aatggaaaat ggagatgtct atacatttgg ttatgggcag1620catgggcagc taggacatgg agatgtcaac tccaggggat gtcccactct tgttcaagca1680ttgccaggcc ctagcacaca agtcactgca ggcagcaacc atacggcagt acttttaatg1740gatggacagg tcttcacatt tggaagtttt tctaaaggac aactgggcag accaattttg1800gatgtgccat attggaatgc aaagccagct cccatgccta acattggatc aaaatatgga1860agaaaagcta cttggatagg tgcaagtggg gaccaaactt ttttacgaat tgatgaagca1920cttattaatt ctcatgtact tgctacatca gaaatttttg ccagtaaaca cataataggc1980ttggtacctg cttctatatc agaacctcct ccatttaaat gccttctgat aaataaagtg2040gatgggagtt gtaaaacttt taatgactca gaacaagagg atctgcaagg atttggtgtg2100tgtcttgatc ctgtatatga tgtaatttgg aggtttcgac caaatactag agagctgtgg2160tgttacaatg cggtggttgc tgatgccagg cttccctctg cagcagacat gcagtccaga2220tgtagtatcc taagtcctga acttgcctta ccaacaggat caagggccct cactacccga2280tctcatgeag ctttgcacat tttaggttgt cttgatacct tggcagctat gcaggactta2340aaaatgggtg ttgcaagtac agaggaagag actcaagcag taatgaaggt ttattctaaa2400gaagattata gtgtggtaaa caggtttgaa agtcatggag gaggctgggg ttattctgcc2460cattcagtag aagctatacg tttcagtgcc gacactgata ttttacttgg tggtcttggt2520ctgtttggag gtagaggaga atatactgct aaaattaagc tgtttgaatt gggtcctgat2580ggaggagatc atgaaactga tggtgacctt cttgcagaga ctgatgtatt ggcttatgac2640tgtgctgcta gagaaaaata tgcaatgatg tttgatgagc ctgttctcct gcaagctggg2700tggtggtatg tggcatgggc ccgagtgtca ggacccagca gtgactgtgg atctcatgga2760caggcatcta ttaccacaga tgatggggtt gttttccagt tcaagagttc aaagaaatca2820aataatggta cagatgttaa tgcgggtcag atacctcagt tattatacag acttccaacc2880
agtgatggca gtgcttcaaa aggcaaacag caaaccagtg aacctgtaca cattttaaag2940aggtcttttg caagaactgt ctcagtggaa tgttttgagt cattgttgag tattcttcac3000tggagctgga ccaccttagt cttaggagtt 3030<210>25<211>1851<212>DNA<213>人<400>25atatttgcca caagcactga acctgttcta cagcaagaat tgcaacttaa actggctaga 60aaatgcttac atgcctgtcg tatctcatta ttcgatctgg aaaaggactt gcatattata 120agtacaggat ttgatgagga gtcagcaatt cttggtgcag gacgagagtt tgcgctaatg 180aaaacagcaa atggaaagat atattacact ggcaaatacc agagtcttgg aatcaaacaa 240ggtggtcctt cagcaggaaa atgggttgag ctaccaatta caaaatctcc aaagatagta 300cacttctcag ttggacacga tggctctcac gcccttttag ttgcagaaga tgggagcata 360ttctttacag gatctgctag taaaggagaa gatggagaat caattaagag cagacggcaa 420tccaaacctt ataaacctaa aaagataatt aagatggaag gaaagattgt ggtatataca 480gcctgcaata atggaagtag ttctgttatt tctaaagatg gagaactcta catgtttgga 540aaagatgcca tttactctga tagttcaagt ttggtaactg atttgaaggg ccattttgta 600actcaggtag ctatgggcaa agctcacact tgtgttttaa tgaagaatgg agaggtgtgg 660acatttggtg taaataataa aggacagtgt ggacgagata ctggtgccat gaaccaaggt 720gggaaagggt ttggagttga aaatatggca acagcaatgg atgaagacct ggaagaagaa 780ctagatgaaa aagatgagaa gtctatgatg tgccctccag gcatgcacaa atggaagctg 840gagcagtgca tggtttgcac tgtctgtgga gactgtacag gttatggagc cagctgtgtc 900agtagtggac ggccagacag agtccccgga gggatctgtg gttgtggttc cggagaatct 960ggttgtgctg tgtgtggatg ttgcaaggcc tgtgcaagag agttagatgg tcaagaggca1020agacaaagag gaattcttga tgcagtgaaa gaaatgatac ctttagatct tcttttagct1080gtcccagtgc ccggggttaa cattgaagaa caccttcagt tacgacaaga agaaaaacgg1140caacgtgtaa tcagaaggca cagattagag gaaggaagag gcccccttgt atttgctggt1200cctattttta tgaaccatcg agaacaggct ctagccagac tcagatccca tccagcacac1260
gtaaagcata aacgggacaa gcacaaagat ggaagtggag aaagaggcga aaaggatgca1320agcaaaatca caacataccc tccaggctct gtgcgatttg actgtgagct ccgggcagtc1380caagtcagct gtggatttca ccattcagtg gttttaatgg aaaatggaga tgtctataca1440tttggttatg ggcagcatgg gcagctagga catggagatg tcaactccag gggatgtccc1500actcttgttc aagcattgcc aggccctagc acacaagtca ctgcaggcag caaccatacg1560gcagtacttt taatggatgg acaggtcttc acatttggaa gtttttctaa aggacaactg1620ggcagaccaa ttttggatgt gccatattgg aatgcaaagc cagctcccat gcctaacatt1680ggatcaaaat atggaagaaa agctacttgg ataggtgcaa gtggggacca aactttttta1740cgaattgatg aagcacttat taattctcat gtacttgcta catcagaaat ttttgccagt1800aaacacataa taggcttggt acctgcttct atatcagaac ctcctccatt t 1851<210>26<211>1701<212>DNA<213>人<400>26ggacgagagt ttgcgctaat gaaaacagca aatggaaaga tatattacac tggcaaatac 60cagagtcttg gaatcaaaca aggtggtcct tcagcaggaa aatgggttga gctaccaatt120acaaaatctc caaagatagt acacttctca gttggacacg atggctctca cgccctttta180gttgcagaag atgggagcat attctttaca ggatctgcta gtaaaggaga agatggagaa240tcaattaaga gcagacggca atccaaacct tataaaccta aaaagataat taagatggaa300ggaaagattg tggtatatac agcctgcaat aatggaagta gttctgttat ttctaaagat360ggagaactct acatgtttgg aaaagatgcc atttactctg atagttcaag tttggtaact420gatttgaagg gccattttgt aactcaggta gctatgggca aagctcacac ttgtgtttta480atgaagaatg gagaggtgtg gacatttggt gtaaataata aaggacagtg tggacgagat540actggtgcca tgaaccaagg tgggaaaggg tttggagttg aaaatatggc aacagcaatg600gatgaagacc tggaagaaga actagatgaa aaagatgaga agtctatgat gtgccctcca660ggcatgcaca aatggaagct ggagcagtgc atggtttgca ctgtctgtgg agactgtaca720ggttatggag ccagctgtgt cagtagtgga cggccagaca gagtccccgg agggatctgt780ggttgtggtt ccggagaatc tggttgtgct gtgtgtggat gttgcaaggc ctgtgcaaga840gagttagatg gtcaagaggc aagacaaaga ggaattcttg atgcagtgaa agaaatgata900
cctttagatc ttcttttagc tgtcccagtg cccggggtta acattgaaga acaccttcag 960ttacgacaag aagaaaaacg gcaacgtgta atcagaaggc acagattaga ggaaggaaga1020ggcccccttg tatttgctgg tcctattttt atgaaccatc gagaacaggc tctagccaga1080ctcagatccc atccagcaca cgtaaagcat aaacgggaca agcacaaaga tggaagtgga1140gaaagaggcg aaaaggatgc aagcaaaatc acaacatacc ctccaggctc tgtgcgattt1200gactgtgagc tccgggcagt ccaagtcagc tgtggatttc accattcagt ggttttaatg1260gaaaatggag atgtctatac atttggttat gggcagcatg ggcagctagg acatggagat1320gtcaactcca ggggatgtcc cactcttgtt caagcattgc caggccctag cacacaagtc1380actgcaggca gcaaccatac ggcagtactt ttaatggatg gacaggtctt cacatttgga1440agtttttcta aaggacaact gggcagacca attttggatg tgccatattg gaatgcaaag1500ccagctccca tgcctaacat tggatcaaaa tatggaagaa aagctacttg gataggtgca1560agtggggacc aaactttttt acgaattgat gaagcactta ttaattctca tgtacttgct1620acatcagaaa tttttgccag taaacacata ataggcttgg tacctgcttc tatatcagaa1680cctcctccat ttaaatgcct t 1701<210>27<211>903<212>DNA<213>人<400>27gctcccatgc ctaacattgg atcaaaatat ggaagaaaag ctacttggat aggtgcaagt 60ggggaccaaa cttttttacg aattgatgaa gcacttatta attctcatgt acttgctaca120tcagaaattt ttgccagtaa acacataata ggcttggtac ctgcttctat atcagaacct180cctccattta aatgccttct gataaataaa gtggatggga gttgtaaaac ttttaatgac240tcagaacaag aggatctgca aggatttggt gtgtgtcttg atcctgtata tgatgtaatt300tggaggtttc gaccaaatac tagagagctg tggtgttaca atgcggtggt tgctgatgcc360aggcttccct ctgcagcaga catgcagtcc agatgtagta tcctaagtcc tgaacttgcc420ttaccaacag gatcaagggc cctcactacc cgatctcatg cagctttgca cattttaggt480tgtcttgata ccttggcagc tatgcaggac ttaaaaatgg gtgttgcaag tacagaggaa540gagactcaag cagtaatgaa ggtttattct aaagaagatt atagtgtggt aaacaggttt600
gaaagtcatg gaggaggctg gggttattct gcccattcag tagaagctat acgtttcagt660gccgacactg atattttact tggtggtctt ggtctgtttg gaggtagagg agaatatact720gctaaaatta agctgtttga attgggtcct gatggaggag atcatgaaac tgatggtgac780cttcttgcag agactgatgt attggcttat gactgtgctg ctagagaaaa atatgcaatg840atgtttgatg agcctgttct cctgcaagct gggtggtggt atgtggcatg ggcccgagtg900tca 903<210>28<211>303<212>DNA<213>人<400>28gctcccatgc ctaacattgg atcaaaatat ggaagaaaag ctacttggat aggtgcaagt 60ggggaccaaa cttttttacg aattgatgaa gcacttatta attctcatgt acttgctaca120tcagaaattt ttgccagtaa acacataata ggcttggtac ctgcttctat atcagaacct180cctccattta aatgccttct gataaataaa gtggatggga gttgtaaaac ttttaatgac240tcagaacaag aggatctgca aggatttggt gtgtgtcttg atcctgtata tgatgtaatt300tgg 303<210>29<211>612<212>DNA<213>人<400>29gatgaagcac ttattaattc tcatgtactt gctacatcag aaatttttgc cagtaaacac 60ataataggct tggtacctgc ttctatatca gaacctcctc catttaaatg ccttctgata120aataaagtgg atgggagttg taaaactttt aatgactcag aacaagagga tctgcaagga180tttggtgtgt gtcttgatcc tgtatatgat gtaatttgga ggtttcgacc aaatactaga240gagctgtggt gttacaatgc ggtggttgct gatgccaggc ttccctctgc agcagacatg300cagtccagat gtagtatcct aagtcctgaa cttgccttac caacaggatc aagggccctc360actacccgat ctcatgcagc tttgcacatt ttaggttgtc ttgatacctt ggcagctatg420caggacttaa aaatgggtgt tgcaagtaca gaggaagaga ctcaagcagt aatgaaggtt480tattctaaag aagattatag tgtggtaaac aggtttgaaa gtcatggagg aggctggggt540
tattctgccc attcagtaga agctatacgt ttcagtgccg acactgatat tttacttggt600ggtcttggtc tg612<210>30<211>117<212>DNA<213>人<400>30gatgaagcac ttattaattc tcatgtactt gctacatcag aaatttttgc cagtaaacac 60ataataggct tggtacctgc ttctatatca gaacctcctc catttaaatg ccttctg 117<210>31<211>382<212>PRT<213>人<400>31Met Gly Pro Thr Ser Val Pro Leu Val Lys Ala His Arg Ser Ser Val1 5 10 15Ser Asp Tyr Val Asn Tyr Asp Ile Ile Val Arg His Tyr Asn Tyr Thr20 25 30Gly Lys Leu Asn Ile Ser Ala Asp Lys Glu Asn Ser Ile Lys Leu Thr35 40 45Ser Val Val Phe Ile Leu Ile Cys Cys Phe Ile Ile Leu Glu Asn Ile50 55 60Phe Val Leu Leu Thr Ile Trp Lys Thr Lys Lys Phe His Arg Pro Met65 70 75 80Tyr Tyr Phe Ile Gly Asn Leu Ala Leu Ser Asp Leu Leu Ala Gly Val85 90 95Ala Tyr Thr Ala Asn Leu Leu Leu Ser Gly Ala Thr Thr Tyr Lys Leu100 105 110Thr Pro Ala Gln Trp Phe Leu Arg Glu Gly Ser Met Phe Val Ala Leu115 120 125Ser Ala Ser Val Phe Ser Leu Leu Ala Ile Ala Ile Glu Arg Tyr Ile
130 135 140Thr Met Leu Lys Met Lys Leu His Asn Gly Ser Asn Asn Phe Arg Leu145 150 155 160Phe Leu Leu Ile Ser Ala Cys Trp Val Ile Ser Leu Ile Leu Gly Gly165 170 175Leu Pro Ile Met Gly Trp Asn Cys Ile Ser Ala Leu Ser Ser Cys Ser180 185 190Thr Val Leu Pro Leu Tyr His Lys His Tyr Ile Leu Phe Cys Thr Thr195 200 205Val Phe Thr Leu Leu Leu Leu Ser Ile Val Ile Leu Tyr Cys Arg Ile210 215 220Tyr Ser Leu Val Arg Thr Arg Ser Arg Arg Leu Thr Phe Arg Lys Asn225 230 235 240Ile Ser Lys Ala Ser Arg Ser Ser Glu Lys Ser Leu Ala Leu Leu Lys245 250 255Thr Val Ile Ile Val Leu Ser Val Phe Ile Ala Cys Trp Ala Pro Leu260 265 270Phe Ile Leu Leu Leu Leu Asp Val Gly Cys Lys Val Lys Thr Cys Asp275 280 285Ile Leu Phe Arg Ala Glu Tyr Phe Leu Val Leu Ala Val Leu Asn Ser290 295 300Gly Thr Asn Pro Ile Ile Tyr Thr Leu Thr Asn Lys Glu Met Arg Arg305 310 315 320Ala Phe Ile Arg Ile Met Ser Cys Cys Lys Cys Pro Ser Gly Asp Ser325 330 335Ala Gly Lys Phe Lys Arg Pro Ile Ile Ala Gly Met Glu Phe Ser Arg340 345 350Ser Lys Ser Asp Asn Ser Ser His Pro Gln Lys Asp Glu Gly Asp Asn
355 360 365Pro Glu Thr Ile Met Ser Ser Gly Asn Val Asn Ser Ser Ser370 375 380<210>32<211>2753<212>DNA<213>人<400>32gtcgggggca gcagcaagat gcgaagcgag ccgtacagat cccgggctct ccgaacgcaa 60cttcgccctg cttgagcgag gctgcggttt ccgaggccct ctccagccaa ggaaaagcta120cacaaaaagc ctggatcact catcgaacca cccctgaagc cagtgaaggc tctctcgcct180cgccctctag cgttcgtctg gagtagcgcc accccggctt cctggggaca cagggttggc240accatggggc ccaccagcgt cccgctggtc aaggcccacc gcagctcggt ctctgactac300gtcaactatg atatcatcgt ccggcattac aactacacgg gaaagctgaa tatcagcgcg360gacaaggaga acagcattaa actgacctcg gtggtgttca ttctcatctg ctgctttatc420atcctggaga acatctttgt cttgctgacc atttggaaaa ccaagaaatt ccaccgaccc480atgtactatt ttattggcaa tctggccctc tcagacctgt tggcaggagt agcctacaca540gctaacctgc tcttgtctgg ggccaccacc tacaagctca ctcccgccca gtggtttctg600cgggaaggga gtatgtttgt ggccctgtca gcctccgtgt tcagtctcct cgccatcgcc660attgagcgct atatcacaat gctgaaaatg aaactccaca acgggagcaa taacttccgc720ctcttcctgc taatcagcgc ctgctgggtc atctccctca tcctgggtgg cctgcctatc780atgggctgga actgcatcag tgcgctgtcc agctgctcca ccgtgctgcc gctctaccac840aagcactata tcctcttctg caccacggtc ttcactctgc ttctgctctc catcgtcatt900ctgtactgca gaatctactc cttggtcagg actcggagcc gccgcctgac gttccgcaag960aacatttcca aggccagccg cagctctgag aagtcgctgg cgctgctcaa gaccgtaatt 1020atcgtcctga gcgtcttcat cgcctgctgg gcaccgctct tcatcctgct cctgctggat 1080gtgggctgca aggtgaagac ctgtgacatc ctcttcagag cggagtactt cctggtgtta 1140gctgtgctca actccggcac caaccccatc atttacactc tgaccaacaa ggagatgcgt 1200cgggccttca tccggatcat gtcctgctgc aagtgcccga gcggagactc tgctggcaaa 1260ttcaagcgac ccatcatcgc cggcatggaa ttcagccgca gcaaatcgga caattcctcc 1320
cacccccaga aagacgaagg ggacaaccca gagaccatta tgtcttctgg aaacgtcaac1380tcttcttcct agaactggaa gctgtccacc caccggaagc gctctttact tggtcgctgg1440ccaccccagt gtttggaaaa aaatctctgg gcttcgactg ctgccaggga ggagctgctg1500caagccagag ggaggaaggg ggagaatacg aacagcctgg tggtgtcggg tgttggtggg1560tagagttagt tcctgtgaac aatgcactgg gaagggtgga gatcaggtcc cggcctggaa1620tatatattct acccccctgg agctttgatt ttgcactgag ccaaaggtct agcattgtca1680agctcctaaa gggttcattt ggcccctcct caaagactaa tgtccccatg tgaaagcgtc1740tctttgtctg gagctttgag gagatgtttt ccttcacttt agtttcaaac ccaagtgagt1800gtgtgcactt ctgcttcttt agggatgccc tgtacatccc acaccccacc ctcccttccc1860ttcatacccc tcctcaacgt tcttttactt tatactttaa ctacctgaga gttatcagag1920ctggggttgt ggaatgatcg atcatctata gcaaataggc tatgttgagt acgtaggctg1980tgggaagatg aagatggttt ggaggtgtaa aacaatgtcc ttcgctgagg ccaaagtttc2040catgtaagcg ggatccgttt tttggaattt ggttgaagtc actttgattt ctttaaaaaa2100catcttttca atgaaatgtg ttaccatttc atatccattg aagccgaaat ctgcataagg2160aagcccactt tatctaaatg atattagcca ggatccttgg tgtcctagga gaaacagaca2220agcaaaacaa agtgaaaacc gaatggatta acttttgcaa accaagggag atttcttagc2280aaatgagtct aacaaatatg acatccgtct ttcccacttt tgttgatgtt tatttcagaa2340tcttgtgtga ttcatttcaa gcaacaacat gttgtatttt gttgtgttaa aagtactttt2400cttgattttt gaatgtattt gtttcaggaa gaagtcattt tatggatttt tctaacccgt2460gttaactttt ctagaatcca ccctcttgtg cccttaagca ttactttaac tggtagggaa2520cgccagaact tttaagtcca gctattcatt agatagtaat tgaagatatg tataaatatt2580acaaagaata aaaatatatt actgtctctt tagtatggtt ttcagtgcaa ttaaaccgag2640agatgtcttg tttttttaaa aagaatagta tttaataggt ttctgacttt tgtggatcat2700tttgcacata gctttatcaa cttttaaaca ttaataaact gattttttta aag 2753<210>33<211>353<212>PRT<213>人<400>33
Met Gly Ser Leu Tyr Ser Glu Tyr Leu Asn Pro Asn Lys Val Gln Glu1 5 10 15His Tyr Asn Tyr Thr Lys Glu Thr Leu Glu Thr Gln Glu Thr Thr Ser20 25 30Arg Gln Val Ala Ser Ala Phe Ile Val Ile Leu Cys Cys Ala Ile Val35 40 45Val Glu Asn Leu Leu Val Leu Ile Ala Val Ala Arg Asn Ser Lys Phe50 55 60His Ser Ala Met Tyr Leu Phe Leu Gly Asn Leu Ala Ala Ser Asp Leu65 70 75 80Leu Ala Gly Val Ala Phe Val Ala Asn Thr Leu Leu Ser Gly Ser Val85 90 95Thr Leu Arg Leu Thr Pro Val Gln Trp Phe Ala Arg Glu Gly Ser Ala100 105 110Ser Ile Thr Leu Ser Ala Ser Val Phe Ser Leu Leu Ala Ile Ala Ile115 120 125Glu Arg His Val Ala Ile Ala Lys Val Lys Leu Tyr Gly Ser Asp Lys130 135 140Ser Cys Arg Met Leu Leu Leu Ile Gly Ala Ser Trp Leu Ile Ser Leu145 150 155 160Val Leu Gly Gly Leu Pro Ile Leu Gly Trp Asn Cys Leu Gly His Leu165 170 175Glu Ala Cys Ser Thr Val Leu Pro Leu Tyr Ala Lys His Tyr Val Leu180 185 190Cys Val Val Thr Ile Phe Ser Ile Ile Leu Leu Ala Ile Val Ala Leu195 200 205Tyr Val Arg Ile Tyr Cys Val Val Arg Ser Ser His Ala Asp Met Ala210 215 220
Ala Pro Gln Thr Leu Ala Leu Leu Lys Thr Val Thr Ile Val Leu Gly225 230 235 240Val Phe Ile Val Cys Trp Leu Pro Ala Phe Ser Ile Leu Leu Leu Asp245 250 255Tyr Ala Cys Pro Val His Ser Cys Pro Ile Leu Tyr Lys Ala His Tyr260 265 270Phe Phe Ala Val Ser Thr Leu Asn Ser Leu Leu Asn Pro Val Ile Tyr275 280 285Thr Trp Arg Ser Arg Asp Leu Arg Arg Glu Val Leu Arg Pro Leu Gln290 295 300Cys Trp Arg Pro Gly Val Gly Val Gln Gly Arg Arg Arg Val Gly Thr305 310 315 320Pro Gly His His Leu Leu Pro Leu Arg Ser Ser Ser Ser Leu Glu Arg325 330 335Gly Met His Met Pro Thr Ser Pro Thr Phe Leu Glu Gly Asn Thr Val340 345 350Val<210>34<211>1062<212>DNA<213>人<400>34atgggcagct tgtactcgga gtacctgaac cccaacaagg tccaggaaca ctataattat 60accaaggaga cgctggaaac gcaggagacg acctcccgcc aggtggcctc ggccttcatc120gtcatcctct gttgcgccat tgtggtggaa aaccttctgg tgctcattgc ggtggcccga180aacagcaagt tccactcggc aatgtacctg tttctgggca acctggccgc ctccgatcta240ctggcaggcg tggccttcgt agccaatacc ttgctctctg gctctgtcac gctgaggctg300acgcctgtgc agtggtttgc ccgggagggc tctgcctcca tcacgctctc ggcctctgtc360ttcagcctcc tggccatcgc cattgagcgc cacgtggcca ttgccaaggt caagctgtat420
ggcagcgaca agagctgccg catgcttctg ctcatcgggg cctcgtggct catctcgctg480gtcctcggtg gcctgcccat ccttggctgg aactgcctgg gccacctcga ggcctgctcc540actgtcctgc ctctctacgc caagcattat gtgctgtgcg tggtgaccat cttctccatc600atcctgttgg ccatcgtggc cctgtacgtg cgcatctact gcgtggtccg ctcaagccac660gctgacatgg ccgccccgca gacgctagcc ctgctcaaga cggtcaccat cgtgctaggc720gtctttatcg tctgctggct gcccgccttc agcatcctcc ttctggacta tgcctgtccc780gtccactcct gcccgatcct ctacaaagcc cactactttt tcgccgtctc caccctgaat840tccctgctca accccgtcat ctacacgtgg cgcagccggg acctgcggcg ggaggtgctt900cggccgctgc agtgctggcg gccgggggtg ggggtgcaag gacggaggcg ggtcgggacc960ccgggccacc acctcctgcc actccgcagc tccagctccc tggagagggg catgcacatg 1020cccacgtcac ccacgtttct ggagggcaac acggtggtct ga 1062<210>35<211>378<212>PRT<213>人<400>35Met Ala Thr Ala Leu Pro Pro Arg Leu Gln Pro Val Arg Gly Asn Glu1 5 10 15Thr Leu Arg Glu His Tyr Gln Tyr Val Gly Lys Leu Ala Gly Arg Leu20 25 30Lys Glu Ala Ser Glu Gly Ser Thr Leu Thr Thr Val Leu Phe Leu Val35 40 45Ile Cys Ser Phe Ile Val Leu Glu Asn Leu Met Val Leu Ile Ala Ile50 55 60Trp Lys Asn Asn Lys Phe His Asn Arg Met Tyr Phe Phe Ile Gly Asn65 70 75 80Leu Ala Leu Cys Asp Leu Leu Ala Gly Ile Ala Tyr Lys Val Asn Ile85 90 95Leu Met Ser Gly Lys Lys Thr Phe Ser Leu Ser Pro Thr Val Trp Phe
100 105 110Leu Arg Glu Gly Ser Met Phe Val Ala Leu Gly Ala Ser Thr Cys Ser115 120 125Leu Leu Ala Ile Ala Ile Glu Arg His Leu Thr Met Ile Lys Met Arg130 135 140Pro Tyr Asp Ala Asn Lys Arg His Arg Val Phe Leu Leu Ile Gly Met145 150 155 160Cys Trp Leu Ile Ala Phe Thr Leu Gly Ala Leu Pro Ile Leu Gly Trp165 170 175Asn Cys Leu His Asn Leu Pro Asp Cys Ser Thr Ile Leu Pro Leu Tyr180 185 190Ser Lys Lys Tyr Ile Ala Phe Cys Ile Ser Ile Phe Thr Ala Ile Leu195 200 205Val Thr Ile Val Ile Leu Tyr Ala Arg Ile Tyr Phe Leu Val Lys Ser210 215 220Ser Ser Arg Lys Val Ala Asn His Asn Asn Ser Glu Arg Ser Met Ala225 230 235 240Leu Leu Arg Thr Val Val Ile Val Val Ser Val Phe Ile Ala Cys Trp245 250 255Ser Pro Leu Phe Ile Leu Phe Leu Ile Asp Val Ala Cys Arg Val Gln260 265 270Ala Cys Pro Ile Leu Phe Lys Ala Gln Trp Phe Ile Val Leu Ala Val275 280 285Leu Asn Ser Ala Met Asn Pro Val Ile Tyr Thr Leu Ala Ser Lys Glu290 295 300Met Arg Arg Ala Phe Phe Arg Leu Val Cys Asn Cys Leu Val Arg Gly305 310 315 320Arg Gly Ala Arg Ala Ser Pro Ile Gln Pro Ala Leu Asp Pro Ser Arg
325 330 335Ser Lys Ser Ser Ser Ser Asn Asn Ser Ser His Ser Pro Lys Val Lys340 345 350Glu Asp Leu Pro His Thr Asp Pro Ser Ser Cys Ile Met Asp Lys Asn355 360 365Ala Ala Leu Gln Asn Gly Ile Phe Cys Asn370 375<210>36<211>1137<212>DNA<213>人<400>36atggcaactg ccctcccgcc gcgtctccag ccggtgcggg ggaacgagac cctgcgggag 60cattaccagt acgtggggaa gttggcgggc aggctgaagg aggcctccga gggcagcacg120ctcaccaccg tgctcttctt ggtcatctgc agcttcatcg tcttggagaa cctgatggtt180ttgattgcca tctggaaaaa caataaattt cacaaccgca tgtacttttt cattggcaac240ctggctctct gcgacctgct ggccggcatc gcttacaagg tcaacattct gatgtctggc300aagaagacgt tcagcctgtc tcccacggtc tggttcctca gggagggcag tatgttcgtg360gcccttgggg cgtccacctg cagcttactg gccatcgcca tcgagcggca cttgacaatg420atcaaaatga ggccttacga cgccaacaag aggcaccgcg tcttcctcct gatcgggatg480tgctggctca ttgccttcac gctgggcgcc ctgcccattc tgggctggaa ctgcctgcac540aatctccctg actgctctac catcctgccc ctctactcca agaagtacat tgccttctgc600atcagcatct tcacggccat cctggtgacc atcgtgatcc tctacgcacg catctacttc660ctggtgaagt ccagcagccg taaggtggcc aaccacaaca actcggagcg gtccatggca720ctgctgcgga ccgtggtgat tgtggtgagc gtgttcatcg cctgctggtc cccactcttc780atcctcttcc tcattgatgt ggcctgcagg gtgcaggcgt gccccatcct cttcaaggct840cagtggttca tcgtgttggc tgtgctcaac tccgccatga acccggtcat ctacacgctg900gccagcaagg agatgcggcg ggccttcttc cgtctggtct gcaactgcct ggtcagggga960cggggggccc gcgcctcacc catccagcct gcgctcgacc caagcagaag taaatcaagc 1020agcagcaaca atagcagcca ctctccgaag gtcaaggaag acctgcccca cacagacccc 1080
tcatcctgca tcatggacaa gaacgcagca cttcagaatg ggatcttctg caactga1137<210>37<211>384<212>PRT<213>人<400>37Met Asn Ala Thr Gly Thr Pro Val Ala Pro Glu Ser Cys Gln Gln Leu1 5 10 15Ala Ala Gly Gly His Ser Arg Leu Ile Val Leu His Tyr Asn His Ser20 25 30Gly Arg Leu Ala Gly Arg Gly Gly Pro Glu Asp Gly Gly Leu Gly Ala35 40 45Leu Arg Gly Leu Ser Val Ala Ala Ser Cys Leu Val Val Leu Glu Asn50 55 60Leu Leu Val Leu Ala Ala Ile Thr Ser His Met Arg Ser Arg Arg Trp65 70 75 80Val Tyr Tyr Cys Leu Val Asn Ile Thr Leu Ser Asp Leu Leu Thr Gly85 90 95Ala Ala Tyr Leu Ala Asn Val Leu Leu Ser Gly Ala Arg Thr Phe Arg100 105 110Leu Ala Pro Ala Gln Trp Phe Leu Arg Glu Gly Leu Leu Phe Thr Ala115 120 125Leu Ala Ala Ser Thr Phe Ser Leu Leu Phe Thr Ala Gly Glu Arg Phe130 135 140Ala Thr Met Val Arg Pro Val Ala Glu Ser Gly Ala Thr Lys Thr Ser145 150 155 160Arg Val Tyr Gly Phe Ile Gly Leu Cys Trp Leu Leu Ala Ala Leu Leu165 170 175Gly Met Leu Pro Leu Leu Gly Trp Asn Cys Leu Cys Ala Phe Asp Arg
180 185 190Cys Ser Ser Leu Leu Pro Leu Tyr Ser Lys Arg Tyr Ile Leu Phe Cys195 200 205Leu Val Ile Phe Ala Gly Val Leu Ala Thr Ile Met Gly Leu Tyr Gly210 215 220Ala Ile Phe Arg Leu Val Gln Ala Ser Gly Gln Lys Ala Pro Arg Pro225 230 235 240Ala Ala Arg Arg Lys Ala Arg Arg Leu Leu Lys Thr Val Leu Met Ile245 250 255Leu Leu Ala Phe Leu Val Cys Trp Gly Pro Leu Phe Gly Leu Leu Leu260 265 270Ala Asp Val Phe Gly Ser Asn Leu Trp Ala Gln Glu Tyr Leu Arg Gly275 280 285Met Asp Trp Ile Leu Ala Leu Ala Val Leu Asn Ser Ala Val Asn Pro290 295 300Ile Ile Tyr Ser Phe Arg Ser Arg Glu Val Cys Arg Ala Val Leu Ser305 310 315 320Phe Leu Cys Cys Gly Cys Leu Arg Leu Gly Met Arg Gly Pro Gly Asp325 330 335Cys Leu Ala Arg Ala Val Glu Ala His Ser Gly Ala Ser Thr Thr Asp340 345 350Ser Ser Leu Arg Pro Arg Asp Ser Phe Arg Gly Ser Arg Ser Leu Ser355 360 365Phe Arg Met Arg Glu Pro Leu Ser Ser Ile Ser Ser Val Arg Ser Ile370 375 380<210>38<211>1566<212>DNA<213>人
<400>38gagtcagccc ccgggggagg ccatgaacgc cacggggacc ccggtggccc ccgagtcctg 60ccaacagctg gcggccggcg ggcacagccg gctcattgtt ctgcactaca accactcggg120ccggctggcc gggcgcgggg ggccggagga tggcggcctg ggggccctgc gggggctgtc180ggtggccgcc agctgcctgg tggtgctgga gaacttgctg gtgctggcgg ccatcaccag240ccacatgcgg tcgcgacgct gggtctacta ttgcctggtg aacatcacgc tgagtgacct300gctcacgggc gcggcctacc tggccaacgt gctgctgtcg ggggcccgca ccttccgtct360ggcgcccgcc cagtggttcc tacgggaggg cctgctcttc accgccctgg ccgcctccac420cttcagcctg ctcttcactg caggggagcg ctttgccacc atggtgcggc cggtggccga480gagcggggcc accaagacca gccgcgtcta cggcttcatc ggcctctgct ggctgctggc540cgcgctgctg gggatgctgc ctttgctggg ctggaactgc ctgtgcgcct ttgaccgctg600ctccagcctt ctgcccctct actccaagcg ctacatcctc ttctgcctgg tgatcttcgc660cggcgtcctg gccaccatca tgggcctcta tggggccatc ttccgcctgg tgcaggccag720cgggcagaag gccccacgcc cagcggcccg ccgcaaggcc cgccgcctgc tgaagacggt780gctgatgatc ctgctggcct tcctggtgtg ctggggccca ctcttcgggc tgctgctggc840cgacgtcttt ggctccaacc tctgggccca ggagtacctg cggggcatgg actggatcct900ggccctggcc gtcctcaact cggcggtcaa ccccatcatc tactccttcc gcagcaggga960ggtgtgcaga gccgtgctca gcttcctctg ctgcgggtgt ctccggctgg gcatgcgagg 1020gcccggggac tgcctggccc gggccgtcga ggctcactcc ggagcttcca ccaccgacag 1080ctctctgagg ccaagggaca gctttcgcgg ctcccgctcg ctcagctttc ggatgcggga 1140gcccctgtcc agcatctcca gcgtgcggag catctgaagt tgcagtcttg cgtgtggatg 1200gtgcagccac cgggtgcgtg ccaggcaggc cctcctgggg tacaggaagc tgtgtgcacg 1260cagcctcgcc tgtatgggga gcagggaacg ggacaggccc ccatggtctt cccggtggcc 1320tctcggggct tctgacgcca aatgggcttc ccatggtcac cctggacaag gaggtaacca 1380ccccacctcc ccgtaggagc agagagcacc ctggtgtggg ggcgagtggt tccccacaac 1440cccgcttctg tgtgattctg gggaagtccc ggcccctctc tgggcctcag tagggctccc 1500aggctgcaag gggtggactg tgggatgcat gccctggcaa cattgaagtt cgatcatggt 1560aaaaaa 1566
<210>39<211>398<212>PRT<213>人<400>39Met Glu Ser Gly Leu Leu Arg Pro Ala Pro Val Ser Glu Val Ile Val1 5 10 15Leu His Tyr Asn Tyr Thr Gly Lys Leu Arg Gly Ala Arg Tyr Gln Pro20 25 30Gly Ala Gly Leu Arg Ala Asp Ala Val Val Cys Leu Ala Val Cys Ala35 40 45Phe Ile Val Leu Glu Asn Leu Ala Val Leu Leu Val Leu Gly Arg His50 55 60Pro Arg Phe His Ala Pro Met Phe Leu Leu Leu Gly Ser Leu Thr Leu65 70 75 80Ser Asp Leu Leu Ala Gly Ala Ala Tyr Ala Ala Asn Ile Leu Leu Ser85 90 95Gly Pro Leu Thr Leu Lys Leu Ser Pro Ala Leu Trp Phe Ala Arg Glu100 105 110Gly Gly Val Phe Val Ala Leu Thr Ala Ser Val Leu Ser Leu Leu Ala115 120 125Ile Ala Leu Glu Arg Ser Leu Thr Met Ala Arg Arg Gly Pro Ala Pro130 135 140Val Ser Ser Arg Gly Arg Thr Leu Ala Met Ala Ala Ala Ala Trp Gly145 150 155 160Val Ser Leu Leu Leu Gly Leu Leu Pro Ala Leu Gly Trp Asn Cys Leu165 170 175Gly Arg Leu Asp Ala Cys Ser Thr Val Leu Pro Leu Tyr Ala Lys Ala180 185 190Tyr Val Leu Phe Cys Val Leu Ala Phe Val Gly Ile Leu Ala Ala Ile
195 200 205Cys Ala Leu Tyr Ala Arg Ile Tyr Cys Gln Val Arg Ala Asn Ala Arg210 215 220Arg Leu Pro Ala Arg Pro Gly Thr Ala Gly Thr Thr Ser Thr Arg Ala225 230 235 240Arg Arg Lys Pro Arg Ser Leu Ala Leu Leu Arg Thr Leu Ser Val Val245 250 255Leu Leu Ala Phe Val Ala Cys Trp Gly Pro Leu Phe Leu Leu Leu Leu260 265 270Leu Asp Val Ala Cys Pro Ala Arg Thr Cys Pro Val Leu Leu Gln Ala275 280 285Asp Pro Phe Leu Gly Leu Ala Met Ala Asn Ser Leu Leu Asn Pro Ile290 295 300Ile Tyr Thr Leu Thr Asn Arg Asp Leu Arg His Ala Leu Leu Arg Leu305 310 315 320Val Cys Cys Gly Arg His Ser Cys Gly Arg Asp Pro Ser Gly Ser Gln325 330 335Gln Ser Ala Ser Ala Ala Glu Ala Ser Gly Gly Leu Arg Arg Cys Leu340 345 350Pro Pro Gly Leu Asp Gly Ser Phe Ser Gly Ser Glu Arg Ser Ser Pro355 360 365Gln Arg Asp Gly Leu Asp Thr Ser Gly Ser Thr Gly Ser Pro Gly Ala370 375 380Pro Thr Ala Ala Arg Thr Leu Val Ser Glu Pro Ala Ala Asp385 390 395<210>40<211>2306<212>DNA<213>人
<400>40gcgcggccca tggagtcggg gctgctgcgg ccggcgccgg tgagcgaggt catcgtcctg 60cattacaact acaccggcaa gctccgcggt gcgcgctacc agccgggtgc cggcctgcgc120gccgacgccg tggtgtgcct ggcggtgtgc gccttcatcg tgctagagaa tctagccgtg180ttgttggtgc tcggacgcca cccgcgcttc cacgctccca tgttcctgct cctgggcagc240ctcacgttgt cggatctgct ggcaggcgcc gcctacgccg ccaacatcct actgtcgggg300ccgctcacgc tgaaactgtc ccccgcgctc tggttcgcac gggagggagg cgtcttcgtg360gcactcactg cgtccgtgct gagcctcctg gccatcgcgc tggagcgcag cctcaccatg420gcgcgcaggg ggcccgcgcc cgtctccagt cgggggcgca cgctggcgat ggcagccgcg480gcctggggcg tgtcgctgct cctcgggctc ctgccagcgc tgggctggaa ttgcctgggt540cgcctggacg cttgctccac tgtcttgccg ctctacgcca aggcctacgt gctcttctgc600gtgctcgcct tcgtgggcat cctggccgcg atctgtgcac tctacgcgcg catctactgc660caggtacgcg ccaacgcgcg gcgcctgccg gcacggcccg ggactgcggg gaccacctcg720acccgggcgc gtcgcaagcc gcgctcgctg gccttgctgc gcacgctcag cgtggtgctc780ctggcctttg tggcatgttg gggccccctc ttcctgctgc tgttgctcga cgtggcgtgc840ccggcgcgca cctgtcctgt actcctgcag gccgatccct tcctgggact ggccatggcc900aactcacttc tgaaccccat catctacacg ctcaccaacc gcgacctgcg ccacgcgctc960ctgcgcctgg tctgctgcgg acgccactcc tgcggcagag acccgagtgg ctcccagcag 1020tcggcgagcg cggctgaggc ttccgggggc ctgcgccgct gcctgccccc gggccttgat 1080gggagcttca gcggctcgga gcgctcatcg ccccagcgcg acgggctgga caccagcggc 1140tccacaggca gccccggtgc acccacagcc gcccggactc tggtatcaga accggctgca 1200gactgacacc ctcggcccac gactgtcttc ccaagtttta cagacttgtt ctttttacat 1260aaaggaattt gtaggaaatg cagccaaagg tgcagtcgga aaagatgcag gggaaatgta 1320tttatgcagc gacaccccac aatgtgaaca aacagacaaa aaatctgtgc cctcgtggaa 1380ttgacgttct gcttgggaac acagaaaaga actcggtgat gaaataatgg agatgattcc 1440agtgacaaac gacagagatg gtgatggtgg tcagggaaga cctctctgca gaggtagtga 1500cttgtgatgt gagctgagac ctctgtcctg ggaagaccaa aagaaaagca tttcaggatg 1560agggaatggc atgcgcaaag gccctgaggc tgaaatgtgc ccatgtgttc taagaaatgc 1620agcgatgctg gtgtgcctgg agcagggacg gagggggaga atgggaggag acaaggagct 1680
gaaggagtag ttcccgaagg accttgtggg tgatatagag gacttcgctt ttgctctgag1740tgaggtggga gccatagaag cttctaagca gaagagggac ttgccctaat tcaggtgatc1800acaggtgtct tgtggcctcc atgggaggtt gaaaaccaca gaaggtgaag gggggctgca1860ctgagccaca ggaacaatga tggagattcc agctaagccc agaccccgtg gattctagat1920agattttaga ggcagcagac agaattactg aggaattgag tgtaagagtg gaataaagtt1980atcaaggaca atgccaaggg tggggcaccc ccaaatttga ctttgggaga ctcagccaaa2040tcctatctgg taataaaatt tcttttttat ttttcttttc tttctttctt tctttctttc2100tttttttttt tttgagttgg gatcttgtgc tctgtcaccc aggctggagt gcaatgggca2160caattatagc tcactgcagc ctggaactcc tgggatcaag cctggagttc ctgcttcagc2220ctccctagta gctgggacta caggcatgca ccaccatgcc cagttaataa aatttcttca2280aatgcaaaaa aaaaaaaaaa aaaaaa 230权利要求
1.S1P或者其功能片段或衍生物在制备药物化合物中的用途。
2.S1P或者其功能片段或衍生物在调节疼痛中的用途。
3.S1P或者其功能片段或衍生物在鉴定调节疼痛的化合物中的用途。
4.减轻疼痛的方法，包括向个体施用足够量的S1P或者其功能片段或衍生物。
5.用作药物的S1P或者其功能片段或衍生物。
6.用作预防或治疗疼痛和/或炎症的药物的S1P或者其功能片段或衍生物。
7.根据权利要求1的用途，其中药物化合物是用于预防或治疗疼痛和/或炎症的化合物。
8.根据权利要求3的用途，其中化合物减轻或消除疼痛。
9.筛选用于调节和/或预防疼痛的药物的方法，其包括步骤a.准备包含PAM或者其功能片段或衍生物并且不包含S1P的样品；b.准备包含PAM或者其功能片段或衍生物并且还包含S1P的第二个样品；c.测定两个样品中的PAM活性；d.将第一个样品与化合物接触；e.检测化合物模拟S1P功能的能力。
10.筛选用于调节和/或预防疼痛的药物的方法，其包括步骤a)准备包含表达S1P受体或者其功能片段或衍生物的细胞的两个样品，和b)将一个样品与化合物接触，和c)测定两个样品中的受体活性。
11.筛选用于调节和/或预防疼痛的药物的方法，其包括步骤a.准备包含表达S1P受体或者其功能片段或衍生物的细胞的两个样品；和b.将样品与S1P接触；和c.将一个样品与化合物接触；和d.测定两个样品中的受体活性或者S1P和受体的相互作用。
12.筛选用于调节和/或预防疼痛的药物的方法，其包括步骤b.准备表达S1P受体或者其功能片段或衍生物并表达PAM或者其功能片段或衍生物的细胞，c.将细胞与化合物接触，f.测定化合物存在条件下的S1P活性；g.测定化合物缺乏条件下的S1P活性；和h.比较根据c)中的S1P活性和根据d)中的S1P活性。
13.根据权利要求10的方法，其中进一步包含一个步骤，其中细胞与S1P接触而不是与化合物接触而且其中将根据以上c)和d)的PAM活性与S1P存在条件下的PAM活性比较。
14.筛选用于调节和/或预防疼痛的药物的方法，其包括步骤a.准备包含S1P或者其功能片段或衍生物并包含S1P受体或者其功能片段或衍生物的两个样品，b.将一个样品与化合物接触，c.测定并比较两个样品中的S1P活性。
15.根据以上权利要求任意一项所述的用途、方法或S1P，其中其功能片段或衍生物能够抑制腺苷酸环化酶活性。
16.根据权利要求10的用途、方法或S1P，其中腺苷酸环化酶是腺苷酸环化酶I型、V型或VI型。
17.根据前述权利要求任意一项所述的方法，其中S1P活性的测定涉及其与S1P受体相互作用和/或活化S1P受体的能力、介导PAM易位的能力、活化PAM功能的能力或者失活AC功能的能力。
18.鉴定调节疼痛的化合物的方法，包括a)选择调节或模拟S1P活性的化合物作为测试化合物，和b)向受试者施用所述测试化合物，和c)测定受试者的疼痛是否得到调节。
19.化合物用于制备调节或预防疼痛的药物的用途，其中所述化合物具有活化至少一种S1P受体的能力和/或具有活化PAM作用的能力。
20.根据权利要求19的用途，其中调节是疼痛的减轻或消除。
21.根据权利要求19或20任意一项所述的用途，其中化合物是FTY720或功能衍生物或者衍生物的化合物的盐，其中功能衍生物优选地是其磷酸化衍生物。
22.减轻或预防疼痛的方法，包括向个体施用足够量的药物化合物，其中所述的药物化合物具有结合和活化至少一种S1P受体的能力和/或具有活化PAM功能的活性。
23.根据权利要求22的方法，其中化合物是FTY720或功能衍生物或者化合物或衍生物的生理可接受盐，其中功能衍生物优选地是其磷酸化衍生物。
全文摘要
S1P或者其功能片段或衍生物在制备药物化合物中的用途。
文档编号G01N33/50GK1838961SQ200480015055
公开日2006年9月27日 申请日期2004年5月15日 优先权日2003年5月30日
发明者M·米夏埃利斯, G·盖斯林格, K·朔利基 申请人:塞诺菲-安万特德国有限公司