人类肝脏表达序列标签j组的制作方法

专利名称：人类肝脏表达序列标签j组的制作方法
技术领域：
本发明涉及生物技术领域，具体地，涉及一类表达序列标签，尤其涉及一类人类肝脏表达序列标签。
背景技术：
肝脏是人体内最大的消化腺。也是体内新陈代谢的中心站。据估计，在肝脏中发生的化学反应有500种以上，实验证明，动物在完全摘除肝脏后即使给予相应的治疗，最多也只能生存50多个小时。这说明肝脏是维持生命活动的一个必不可少的重要器官。肝脏的血流量极为丰富，约占心输出量的1/4。每分钟进入肝脏的血流量为1000-1200ml。肝脏的主要功能是进行糖的分解、贮存糖原；参与蛋白质、脂肪、维生素、激素的代谢；解毒；分泌胆汁；吞噬、防御机能；制造凝血因子；调节血容量及水电解质平衡；产生热量等。在胚胎时期肝脏还有造血功能。
肝脏疫病分为肝炎、肝硬化、脂肪肝、肝癌等。现代医学实验证明，肝病病毒侵入人体后，并不直接引起肝细胞的损害，只是在肝细胞内吸收营养赖以生存，并在肝细胞内复制、繁殖。其复制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)释放在肝细胞膜上，引起人体免疫系统对这些抗原物质产生免疫反应，这种反应造成肝细胞的损伤、坏死。免疫反应的强弱决定于肝脏受损程度及临床症状轻重。这场由病毒引发的、免疫系统对肝细胞的战争，使大约25％的患者的肝脏成为战火连绵的战场，肝脏的损伤由此加重。肝病的危害绝不仅仅限于肝脏本身，它还可以引起其它多种疾病。常见的有(1)糖尿病；(2)胰腺炎；(3)胆道感染；(4)功能性肾衰竭；(5)胆汗性肾病；(6)肾小球肾炎；(7)肾小管酸中毒；(8)溶血性贫血；(9)再生障碍性贫血；(10)心肌炎和心包炎；(11)结节性动脉炎；(12)消化性溃疡；(13)自发性腹膜炎；(14)性激素代谢紊乱；(15)甲状腺功能改变；(16)肝性骨病，等等。肝病不仅对患者的身体甚至生命造成危害，而且对患者心理上的打击也是十分沉重的。无论是肝病患者还是病毒携带者，在生活、社交、求职、升学等方面都会受到严重影响。
生物基因组中可转录表达的序列(即基因)仅占总序列的3-5％，对这部分序列进行测定，将直接导致新基因的发现，并获取基因组中与产业化关系最为密切的信息。20世纪80年代，高通量的自动测序的出现，使从质粒互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库随机选取许多cDNA克隆和决定来自非载体两端的几百个碱基的DNA序列成为可能。这些短的DNA序列叫做“表达序列标签”(Expressed Sequence Tags，简称ESTs)。表达序列标签的概念最早是由Adams等在1992年提出来的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics，2，173-179)针对获得大量信使核糖核酸(mRNA)序列的迫切需要，提出大规模互补脱氧核糖核酸(cDNA)测序的研究战略。随后Venter创立了大规模表达序列标签技术。其基本特征就是从以质粒为载体，构建完成的目的组织互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库中，随机选择许多cDNA克隆，利用质粒上携带的通用引物对cDNA两端进行一轮脱氧核糖核酸序列测定，所获得的来自3’端或5’端的几百个碱基的非载体短脱氧核糖核酸(DNA)序列。简而言之，表达序列标签是来自表达基因片段3’端或5’端的短脱氧核糖核酸序列，代表一个表达基因的部分转录片段。
表达序列标签可用于新基因克隆、人类基因组图谱绘制、基因组序列编码区的确定等。如果一个表达序列标签在基因组中只出现一次，那么它可以作为序列标签位点(STS)。由表达序列标签构建的物理图谱叫表达图或转录图(expression ortranscript map)。利用表达序列标签进行基因图制作，可以加快序列标签位点的制作和新基因的染色体定位。表达序列标签可以作为基因特异性探针，对组织特异性基因表达的研究具有重要的作用。表达序列标签还可以进行新基因的遗传进化关系分析。表达序列标签可以对所有动植物的基因作为一种数据库，通过不同的序列比较可以获得保守序列片段，从而获得基因的遗传进化图谱。正因为表达序列标签具有如此的优越性，因此表达序列标签测序已经成为许多基因组研究机构的工作重点。
由于本发明人类肝脏表达基因与一些肝脏疾病相关，因此，研究人类肝脏表达序列标签对探索肝脏疾病的发病机理及研制肝病的治疗药物具有重要意义。

发明内容
本发明要解决的技术问题是提供一类人类肝脏表达序列标签。
本发明要解决的技术问题通过如下技术方案实现本发明提供一类人类肝脏表达序列标签的序列，其包括(a)SEQ ID No.1～SEQ ID No.50所示的序列；(b)SEQ ID No.1～SEQ ID No.50所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.50所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.50所示的序列。
本发明还提供了一种探针分子，所述的探针分子含有上述序列中约8-100个连续的核苷酸。
由本发明的在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物中发挥重要作用。
具体实施例方式
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不是限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件如Sambrook等人，分子克隆实验室手册(New YorkCold Spring HarborLaboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1人肝脏组织的mRNA的分离组织分离(Tissue isolation)肝脏来源于5个成年男性，在肝脏切除手术后，将肝脏组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation)取出肝脏组织，用研钵研碎，加入盛有裂解液的50ml管，充分振荡后，再移入玻璃匀浆器内，匀浆后移至50ml新管，抽提总RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligod(T)的纤维素柱分离总RNA中的mRNA，定量。
实施例2cDNA文库的构建(Constuction of cDNA library)以mRNA为模板，合成双链cDNA。补平末端后，加含EcoRI切点的接头。磷酸化EcoRI末端后，用XhoI限制性内切酶消化1.5小时，再进行片断分离。过柱筛选长度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，无菌水溶解，连接至Uni-ZAP XR载体(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)进行包装，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)细菌。涂板并测定滴度。
实施例3测序及数据库建立(Seqencing and Database Constructing)挑选文库中有外源片段插入的克隆，扩增后抽提质粒(Qiagen Germany)，用T3和T7作为3’和5’端的通用引物，采用终止物荧光标记(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377测序仪(Perkin-Elmer，USA)上进行EST大规模测序。测序结果用FACTURA软件去除载体序列，传输到SUN Ultra 450Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group，USA)中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL)，将无同源性或同源性低于95％的序列视为新基因建立数据库。
实施例4基因的全长克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基础上，进行cDNA全长克隆，分两阶段进行(1)“电子克隆”(Electronic Cloning)以新基因片段序列作为探针搜寻dbEST数据库，将重叠序列＞50bp，同源性在98％以上的表达序列标签(Expressed Sequence Tag，简称“EST”)序列认为同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER软件进行连接，部分EST可以延伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(OpenReading Frame，ORF)，用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸和氨基酸水平上是否与其他物种有同源性，以帮助判别所得到的基因全长完整性如何。通过电子克隆的方法，通常可获取人肝脏相关基因的全长序列。
(2)cDNA术端快速扩增(Rapid Amplification of cDNA Ends，RACE)如果通过“电子克隆”方法仍未得到完整的cDNA全长，则在已有序列5’或3’端设计引物，在人类肝脏Marathon-Ready cDNA文库(Clontech Lab，Inc，USA)中进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软件分析被延长的序列有无完整的ORF，如无，重复上述过程直至获得全长。
(3)RT-PCR对于5’和3’端的已知的序列，如果中间有一段间隙(gap)无法从已有的公共数据库或自身数据库获得，可考虑采用RT-PCR的方法。在序列5’端设计引物，3’端引物采用Oligo-dT，在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最后拼接便获得全长。
通过组合使用上述3种方法，可获得人肝脏相关蛋白的全长编码序列。
序列表<110>上海人类基因组研究中心<120>人类肝脏表达序列标签J组<130>NP-10040<160>50<210>1<211>581<212>DNA<213>Homo sapiens<400>11 ttggctccca aagtcatttt atttaacaaa ggggtcaagg cagaggaaag tttcccttaa61 tatccccaca actgctccac atgtcttctg tggaaacact tcaccaggaa ctagctcaac121 actcttgcta acaatttagt gtctatacag gaaggctcgg tgtctctgtt acaggtggcc181 cgttccttaa agcctttagg gttaatcgca gctgcactga gtggccaagc agaccctgtt241 gggantgtga aagcagtttg ttaacagggc ccctggcggc gcccacgacg gctgtcagac301 tcagcaagta acactgaatg tccaaaaata cggctgtgtt aaactaacaa gccaatcctt361 ctgctcagat ctctggatag aaatgatttt tcttttatct atggggaatg caatttcatc421 acagcccctt acataaacgc tcctgaaacc ctttcagtag acagcatttc aattcagaga481 ccaaagtgaa actatctttg aaaacaggga cgtggctggg aaaccatgca cacctcggcg541 aacaattttt cacccacacg agcttggact ttctgggaan t<210>2<211>268<212>DNA<213>Homo sapiens<400>21 tcggttttta aaaatctttt tattgatatc tcgttttctt catgcatcac ttctaatatt61 cttttnttgt ctatgttctg ttttttttca ttaagcactt ttttntaatt acattttaat121 gtaattagaa tgccaccttt acaatacaca aatacagtaa cggtaactcg cactaaaaca181 aagcatactt ctgatagcca ttattttttt ctttgggnca aatttaaagt tttttttttg241 tcacaaaaac aggaatgtac ncatacaa<210>3
<211>444<212>DNA<213>Homo sapiens<400>31 gagncnatct cgtttatttt ttgtatatac atctacatac attttataca ttatattcat61 atatacatat atacaaatgt atatatgttt aaagccacgc tataagagga agtaaatgag121 aaacagacaa atgaagtggt agctggagga cgtgggaatc agtacaggtt ttcacgcctg181 tttcagaaat gggagattct acagcacgct cgacactaac acatcacatc cataacatat241 cacatccaat agacaggaca gccaaaaaca caaaacaaaa aaagaatcaa atacccacac301 ccagacacaa tactcaacaa cacaaatacc cgaccaaagt ctttaaggaa aggcaaaaga361 ggctggggca cagtgggctc acacctgtta atcccaggca ctctaggggg gccaaggtgg421 ggtgggaccn cttgagggtc aggg<210>4<211>1023<212>DNA<213>Homo sapiens<400>41 aactcccgga gaatccgaat tcgtggtcct cagaattggt ttagggttgt ttcgcggttc61 ttcccgtatt ctgcctccga ggcccagtgg ggctggctgt gggccccctg tgctgtttga121 ggcgtgaaac tcacctcagg aaatgtatct gatccattag cgtggattca tattatttgg181 tgatggagaa tcttcagctg tgttaagtgt taccgtcagt ttcagagatg gcttctttgc241 aaaggaaagg gctgcaggca aggattctca cctctgaaga agaggagaaa ctgaaaagag301 accaaacttt ggtgtctgat tttaaacagc agaaattgga acaagaggct cagaaaaatt361 gggatctttt ttacaaaaga aatagcacta atttcttcaa agacagacac tggaccacca421 gagagtttga ggagctaaga tcatgtagag agtttgaaga tcaaaagtta acaatgcttg481 aagctggctg tggggttgga aactgtttat tcccactttt agaagaagat ccgaatatct541 ttgcctatgc ctgtgatttt tctccaagag ccattgaata tgttaagcaa aatcctttat601 atgatacaga aagatgcaag gtattccagt gtgatctgac taaagatgat cttctggatc661 atgtaccgcc agagtctgtg gatgttgtta tgttgatatt tgtgctgtca gctgttcatc721 ctgataagat gcaccttgtc ttacaaaaca tttacaaggt attaaaacca ggcaaaagtg781 tcttgtttcg tgactacgga ctgtatgatc atgccatgct taggtttaaa gccagcagca841 aacttggaga aaacttttat gttagacaag atgggaccag atcatatttt tttactgatg901 acttcctggc tcagctcttt atggacacag gttatgaaga agtggtaaac gagtatgtgt961 ttcgagagac ggtgaataaa aaagaaggcc tgtgaaaaaa aaaaaaaaaa aaaaaaaaaa1021 aaa<210>5<211>766<212>DNA
<213>Homo sapiens<400>51 gcgagccccg gcgcaggggc cggatctggc cgggggccgg cggcggtgtg ggagcggcgc61 gtcatgtaca ccatcaccaa ggggcccagc aagctggtcg cgcagcgccg cacaggtccc121 acgcagcagc aggtggaggg ccggctcggc gagctcctga aatgccggca gcccgcgccg181 ccgacctcgc agcccccgcg ggcgcagccc tttgcgcagc cgccgggacc ctggcccctg241 tcgagcctgg cagcaggtgc aacagcagct ggatggtggc ccagccggtg agggcgggcc301 aaggcctgtg cagtacgtgg agaggacccc caatccccgg ctgcagaact ttgtgcccat361 tgacctagac gagtggtggg cgcagcagtt cctggcgaga atcaccagct gttcctagtg421 gctgctggga gggggcgctg ctacacggcc gacctgtcgc caggagagaa gcatggcgcc481 ctgcccaccc actgcgcctg gctgggtgcc ggccacacct gaagtgccag catttggact541 tttgcacctt tttttccctt ggcccggctg tcccaaccaa gctgccatgg ccaagggccg601 aacccgtctg acctcagccc tgctcactgt gcccagggac cagcgaccag cccctggggc661 tggcagggag gagctccagg ctaataaagt ggagaaactg tcaaaaaaaa aaaaaaaaaa721 aaaacaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa<210>6<211>692<212>DNA<213>Homo sapiens<400>61 cccgagacct cccggcagtc ttccgagcaa gatggcgccg cgggcatttc ttccactgcc61 cgtctgaggg aacgctaagt agtgtgtccg gcgccgtgtt ccagctccgc gttgttccgc121 gagaaagcga gaggccgagc ccgggctggt gcgatggccg cggtggtggc caagcgggaa181 gggccgccgt tcatcagcga ggcggccgtg cggggcaacg ccgccgtcct ggattattgc241 cggacctcgg tgtcagcgct gtcgggggcc acggccggca tcctcggcct caccggcctc301 tacggcttca tcttctacct gctcgcctcc gtcctgctct ccctgctcct cattctcaag361 gcgggaagga ggtggaacaa atatttcaaa tcacggagac ctctctttac aggaggcctc421 atcgggggcc tcttcaccta cgtcctgttc tggacgttcc tctacggcat ggtgcacgtc481 tactgaaatg ggggcccggg ggactttttt aaaaaaccag atcgggagga ctgtggccag541 caattaacac catgtagact tccttagttc ttaagtggtt gaattcgctg cttgttctgt601 aacgttataa ataatttata tctgaagacg gagagcctgt aatattcttc agattaaatg661 aagcgtgaga cacttaaaaa aaaaaaaaaa aa<210>7<211>1406<212>DNA<213>Homo sapiens<400>7
1 ggccgccctg ccgcgcgcac atgtaggcgt tccgagcggc ggcggaggtg agcgcacgga61 cgagcgggag ggacccttct ccggcctgat gcgacccgat tgtccgcagt gactacactc121 atggcaggtc ccctgtggcg gaccgcagca tttgtgcaga gacacaggac aggcctcttg181 gtgggttcct gtgcaggcct gtttggagtt ccagtctcgt accacctctt cccggatccc241 gtggtccaat ggctctacca gtactggcct cagggccagc cagctccgct ccctccacag301 ctgcagagcc tcttccaaga ggtgctacag gacataggtg ttccttcagg ccattgctac361 aagcccttca ccaccttcac cttccagcct gtgagtgcag gcttcccaag actccctgct421 ggggctgtgg tgggcatccc tgccagtttc ttgggagacc tagtgatcaa cactaaccat481 cccgtggtca tacatgggca tacagtggac tggcggagcc cagcaggcgc ccggctgaga541 gcttccctga ccttgtcccg tgaagcccag aagttcgcct tggccaggga agtggtgtac601 ctggaaagca gtaccactgc cgtgcacgcc ctgctggccc cagcttgcct ggcagggacc661 tgggcactgg gcgtgggtgc caagtacacc ctggggctcc atgcaggccc catgaattta721 cgggctgcct tcagcttggt ggcagcagtg gcaggctttg tggcctacgc cttctcccag781 gattctctca ctcatgccgt ggagtcctgg ctggaccgcc gcacggcctc cctctctgca841 gcctatgcct gtggtggagt ggagttctat gagaagcttc tgtcgggcaa cctggccctg901 cgcagtctct tgggcaaaga gggggagaag ctgtatacac ccagcgggaa catcgtcccc961 agacacttgt tccgaatcaa acatttaccc tacaccaccc gccgggactc tgtgctgcag1021 atgtggaggg ggatgctcaa tccgggccgc tcctgatggg ctcatcacaa ggacacttcc1081 agcttgtgca gacaccaccc tgccattgag tctggagggc cctgttggag cctttggacc1141 tatagctcaa ggccagaaaa atcactggct ttggaattaa atagcttaga ttgtactata1201 accactactt atgaactcag ggactatgag ggactattca ggggctatga atctgagcct1261 ttgtttcttg aactgtaaag tggagatgat gtaaaccgcc ttgcaagatt gtagagttgg1321 gtaaggtcat gaacataagg gcctggcaca aagggtgcac tgtaaataaa cagacatccc1381 tccttaaaaa aaaaaaaaaa aaaaaa<210>8<211>1379<212>DNA<213>Homo sapiens<400>81 gcggccggga ggccggagca gcacggccgc aggacctgaa gctccggctg cgtcttcccg61 cagcgctacc cgccatgcgc ctgccgcgcc gggccgcgct ggggctcctg ccgcttctgc121 tgctgctgcc gcccgcgccg gaggccgcca agaagccgac gccctgccac cggtgccggg181 ggctggtgga caagtttaac caggggatgg tggacaccgc aaagaagaac tttggcggcg241 ggaacacggc ttgggaggaa aagacgctgt ccaagtacga gtccagcgag attcgcctgc301 tggagatcct ggaggggctg tgcgagagca gcgacttcga atgcaaccag atgctagagg361 cgcaggagga gcacctggag gcctggtggc tgcagctgaa gagcgaatat cctgacttat421 tcgagtggtt ttgtgtgaag acactgaaag tgtgctgctc tccaggaacc tacggtcccg481 actgtctcgc atgccagggc ggatcccaga ggccctgcag cgggaatggc cactgcagcg541 gagatgggag cagacagggc gacgggtcct gccggtgcca catggggtac cagggcccgc601 tgtgcactga ctgcatggac ggctacttca gctcgctccg gaacgagacc cacagcatct661 gcacagcctg tgacgagtcc tgcaagacgt gctcgggcct gaccaacaga gactgcggcg
721 agtgtgaagt gggctgggtg ctggacgagg gcgcctgtgt ggatgtggac gagtgtgcgg781 ccgagccgcc tccctgcagc gctgcgcagt tctgtaagaa cgccaacggc tcctacacgt841 gcgaagagtg tgactccagc tgtgtgggct gcacagggga aggcccagga aactgtaaag901 agtgtatctc tggctacgcg agggagcacg gacagtgtgc agatgtggac gagtgcgcac961 tagcagaaaa aacctgtgtg aggaaaaacg aaaactgcta caatactcca gggagctacg1021 tctgtgtgtg tcctgacggc ttcgaaggaa cggaagatgc ctgtgtgccg ccggcagagg1081 ctgaagccac agaaggagaa agcccgacac agctgccctc ccgcgaagac ctgtaatgtg1141 ccggacttac cctttaaatt attcagaagg atgtcccgtg gaaaatgtgg ccctgaggat1201 gccgtctcct gcagtggaca gcggcgggga gaggctgcct gctctctaac ggttgattct1261 catttgtccc ttaaacagct gcatttcttg gttgttctta aacagacttg tatattttga1321 tacagttctt tgtaataaaa ttgaccattg taggtaaaaa aaaaaaaaaa aaaaaaaaa<210>9<211>822<212>DNA<213>Homo sapiens<400>91 ggagggggca tggcggatac tgtgttgttt gagtttcttc acacggagat ggtggctgag61 ctgtgggctc acgaccccga ccccggcccg ggggtgagcg ccgggctccg tggggaggaa121 gcgggggcca ccaagggaca gaagatgagc ctgtcggtcc tggagggtat ggggttccgt181 gtgggccagg ctctaggcga gaggctgccc cgggagacgc tggccttcag ggaggagctg241 gatgtcctca agttcttgtg caaagacctg tgggtggcgg tgttccagaa gcagatggac301 agcctgcgca ccaatcacca ggggacctac gtcctgcaag acaacagctt ccccctcctc361 ctcccgatgg cctctggcct gcagtatctg gaggaagcac ccaagttcct ggccttcacc421 tgcggcctcc tgcgcggcgc cctctatacc ctgggcattg agagcgtggt caccgcctcc481 gtggcagccc tgcccgtctg taagttccag gtggtgattc cgaaatccta agcctgcctc541 gcacctgctg agccgcactg ctgcccctgg cctcaccggc ctcaggagac ggtggggccg601 ctgcatttcg gggtggtctt gggaatccca ggccctaggg gtcacatttg ctcaggaagt661 gggtatcaaa ttgaggtggg ggtgtcagag gaggcaaagg ggtcccagct gcggtcagga721 ctgtggtggg attcccgcct cggcagcgtc actgcccagt agctgctcaa taaaggctgt781 gtgtgtggaa tctgaaaaaa aaaaaaaaaa aaaaaaaaaa aa<210>10<211>613<212>DNA<213>Homo sapiens<400>101 tcctggtgtt tcaaatattt attttaggta gtaaaggctt ttgtcgttct aaatcctgat61 tacaagtcac atgatcaaga acaactaata aaggagattc aggagcatgt taaaaaaact121 acagcacctt acaaatatcc cagaaaggna ggggnnnccn aattataacg aatatttgcn
181 cagtgttgtg gacaatttca ngngtttatt tttacatttt aatgaatatt ttcttcacac241 atgctgcacc acatgtccca aactgaactg atgacatatg ggtaagaatc agaatctttg301 agattaaaat gaaaccctga aacctatttt ggtttgggtc ctttggctta ctggactcac361 agtaaatact taatatccaa gacaactttc ctaacnatac cctttttccc nattttgggc421 tgttacccca aacccattcc caaggactaa ttcnaattct tccacctaga attcntaatc481 caaactggcc aaaanaattg ggccttatgt aagntaaaaa nttccgggcn aatttcctcc541 cccggatgcc aanggtggaa tttntccagg gggccaagnc tatcgnggga aggcaaaagg601 aatgacnggg ggn<210>11<211>607<212>DNA<213>Homo sapiens<400>111 tcaaatgaca caattcagct ttatttttac ttaattataa caatttttaa aaactccatg61 actttgtgct atttctaata tttaaataaa aaacatttca aattttgcac aaataattta121 ggccaataca taactagatt tgaataaagt cagatgaagc aataattcct cctctgtgtt181 tgaaaggaat gagtgtggtt acaaagtcac aggatgagtc cctgggatct ggggtgggag241 aaggggtgga tcaagaatga cttgggcttg tcactcccta gcaggctgag ggcgtgacac301 agcagctcgg tggcggagag gtctattcta gtttctaaca ctccaatgct aactttttgg361 atgtatttcc ttctagcatg tagaaaaggg cttttcttgg ctgccaggaa gtagggagca421 gggatgtggc atggtgatga tctgaggaca gccaggcata tgctcagaca ctttggaaaa481 ctgggggaag gggaagcagg gagacagaat cttcatctnc ttccttttgt gaactgggaa541 gaagggtnct tggtgacatt tccngagtat aaagangaat acaggttgaa aaggttgtaa601 tggtaaa<210>12<211>617<212>DNA<213>Homo sapiens<400>121 gaattagaaa atccatttta ttgcttgggt ttaaaatagt tgtgggatac aagtatttac61 aatgctattg gagtcaatta ttgacaacac tttgcaacag taataccatt tctagctttt121 caattggcaa tacttagaac cttactgtag tgacctgatt ttaaatacca tattatattt181 actaagttaa gagctagttt ttactctctt ccataatttc attacatgaa tgtaagatga241 tggctcaaaa atgacgactt atagtttgaa tttatgtgta tgcaatatac atatgagaac301 caaattcaac aagtgacatg aatgttacta catgaacatt gaattgtatt gcccttgtca361 gttatttcct ctggttcaat aaatactgaa ggtcacaaac acctttttac ttttcaagag421 tttgccttct cttctcgatt ttagtaatta atttggatat ttttcctccc atgcctcttc481 atctgattta gtgggatgtt ttccatacca gcaaaaccaa agaacaggaa aagtatttgg
541 caactcatcn actggganct aatctcccta agggtccaag caaaangggc cattccaatt601 ccaagagcgg tccttaa<210>13<211>693<212>DNA<213>Homo sapiens<400>131 agaggacaca ttacagatga cgttcaggga ctggggaaga agggaaaaaa gcaagaaatg61 ttgagttcaa gatgtctgtg aagatccaaa tggctatgtc tacctgccag atgggatgta121 taaattagtc agggctagag acatagattt gaataatcag atttgaaaca cccttgtaag181 ggtagttcag actcaaaagt atataagatg ccctaggaaa atatgtagag aaagaacaat241 aggacaagga tgaaatcctg gagaatacca attatcacag aggtggaaag agaaggaata301 tagagacatt tgtgaaagag caattataaa tatatgatag agactgccag tgtccattaa361 tattggtgtg cctctcttct tccataaaag tactgctaaa ctacatatct cagcctttgc421 agttaggtaa agccatttga aaatattttg tccaaagatc tatgggcaga agtaatatat481 gcacttctag gcctggtttc tgaaacttct tccatgatcc tctgtgctgt ctcatttttc541 atggaagact tggaagccac aaactgagga agccaagtct ctgaatgtct ctaaggagct601 gcacttagga cagacactgt agtaaatatc aatattttgt gtcagaggtc taaaccctga661 aaaacctaca tggtttgcac cacaaaaaaa aaa<210>14<211>454<212>DNA<213>Homo sapiens<400>141 tttagantaa tntcaccgta atttattcat ctcgcatact cagcccaaca ggggatgaca61 ctgcatctgt gcaggtctac ccgggggttc actctggagc acagggtcgg gaggcaggct121 tggtagaaat cagagggtgg ggtaacccct tgtcccttga gggatatgct tgactaattc181 aaagagttta gcagtctgtt gggggcatga aagccattag gctgaggacc tgggtgggat241 gcagcatgaa attttaggtg tcacagtaca attgaccctg atgctttaac atcagacatt301 tattttaatg atgactaata ttttgagagg ctgagagaag gctnttgaga cattgtaata361 agtgcttagg ggggcatgag acattaggga nggccacaat tattgagtaa tgaaatgtgg421 gagggccgat gaggaagcta ctgntcccct tttt<210>15<211>508<212>DNA<213>Homo sapiens
<400>151 ctttcagcag canagcagca cctgctcatt nacttnncgc ggtcanccgn ncncncctgc61 gtgcagcccc aaggaggagg cgcgctagtg gactggacct cagcggaccc gtggtggtgc121 gggggatgtt tgcggggtgt gtgtggggag tgggggtggc caggattgct ggctcctaga181 accccttaga gcgggaccgg tggattcctt aaggctctga agggctgaga acagttctag241 aagcgtccca aaggtngctg ggaaaggtcc cagcgtgggt tgagggagga gggaagatcc301 gagttcctca ccgcgggccg ggatgtgacc accctcttca gaggttggac cccaggcttc361 aagcaccgag tgaggggtct gtttggggac gttgggagat cttttggtgt taaagttggn421 ttaggtcttg ganttgtcaa gnttttgcac cagccgttcc tgcccacgtt ttaaggattg481 gccccgggtt nccctttttg gaagaatt<210>16<211>674<212>DNA<213>Homo sapiens<400>161 tttttttttt ttttttttgc aggtagtaat acatattttt atctagaaaa gaaatttggt61 ttacagaggc ataaacattt agtagttacg tttatcaatg tttgtttcat aagagaagcc121 tcagtttctt cactgagtca tctgtacatt ttttattcct taccatcaat aattctcagg181 actgtcagac gcctttacaa gaaacatgta gggttttttt ccccccaatt tgagtacata241 ctactgttaa ctgcaaaatt ctaaagaagt cagttgccat ttgtcaaaca attcgcccaa301 ggtacaccga tttgggatac ttttctttca gagccggcca ctgaacaatg aagtaatctg361 agagatgaaa cagaatcttt ccggacatgg ataacgtttc tacacggcag atgctttcaa421 cgtagacaat gatcactttc tttattccta gtatcccaag gagaagggca gatacacaga481 taggaacaca tgttcctggt ccgttacaca acaccaaatc tggcttcacc ctgtgaatta541 ggggaaagga gagccacatg gagtgcaagg tggtgaaaac ggtggagggc caggactgct601 gaacctcccg gcttcttggg attcggtgaa tgtagtattt ggtatacatg ttactagggt661 ctctatcagc cctc<210>17<211>440<212>DNA<213>Homo sapiens<400>171 ggagcggccg cacgctagtg tttttttttt tttttttttc tttctttgag acggagtctt61 gctctgtggc tcatcctgga gcacagtggt gcgatatcag ctcactacca cctccgcctc121 ctgggttcaa gcgactgtcc tgcctcagcc tcccgagtgg ctgggattac aggcacacat181 caccacgcct ggccaatttt tgtatttgta gtatagacag ggtttcactg cctgcctcag241 cctcccatag tgctgggatt acaggcatga gcctccgtgc ccggtgcatc cctaatcttg
301 agcatgatct cagtcggcaa atgaggccat ctgttttcac cctgtttgaa aataagatgt361 ggggaggcca tgatggaaat acccacgtgg ggttaaacat aactggcaaa tgtgggagcg421 atggtggggc atgccattca<210>18<211>840<212>DNA<213>Homo sapiens<400>181 gactagttag gtcagtattt ttagaaactc ttaatagctc atactcttga taccaaaagc61 agccctgatt gttaaagcac acacctgcac aagaagcagt gatggttgca tttacatttc121 ctgggtgcac aaaaaaaaat tctcaaataa gcaaggactt acgctttttg caaagccttt181 gagaagttac tggatcatag gaagcttata acaagaatgg aagattctta aataactcac241 tttctttggt atccagtaac agtagatgtt caaaatatgt acgctgatta ataccagcat301 tgtgaacgct gtacaacctt gtggttatta ctaagcaagt tactactagc ttctgaaaag361 tagcttcata attaatgtta tttatacact gccttccatg acttttactt tgccctaagc421 taatctccaa gatctgaaat gctactccaa tatcagaaaa aaagggggag gtggaattat481 atttcctgtg attttaagag tacagagaat catgcacatc tctgattagt tcatatatgt541 ctagtgtgta ctaaaagtca agatgaactc taaaagaaga agagaaaaca caaacacaaa601 aaagagtgtg tgcgcggcct acggccctgg tgaagggtaa agaacactca cgtgtggggc661 gcgcgccccg aaggaagggc acagtgggac aaaaagggac ccgagaaaac acgaggatag721 aaagaggagg ggaatccgcc gcaaatgaaa aacgcccggg gaaaacaaag gtttgaaaag781 acacagaagg agacgtgagg aaaaagggaa cgacaaaaga tcacagggca caacgaaagc<210>19<211>792<212>DNA<213>Homo sapiens<400>191 tgttgcgggg ttggtatgaa attaaatctt ggtgatgtga ccctacattc tcttctggtc61 cctagaatcg gcttctggtc tcctgataac tgaagtggag acagaagttg agcctgttgc121 ccaggcaaac taaagctgct tttgttcttc ggaatctgct ttgcctccgt cagcctgctt181 ccttccccac acatgctggc cgcactgtcc ccactccaga cctctgctgt gtgtcctggg241 cagggccgcg ttttggcagt accctttcaa ctcatcctaa gcttcgtgta gattacttta301 gtatatattt tttataaaac ataaagcctt tcctctcgat ggaaatcaaa gcttaccatg361 tgagcactcg aacttctaag ttgtgacagg aataacaaaa ctgcaaggag tggaaaagat421 ggaaaagcct gtgggaaatc cgaggccttt tgaaagaagg gagctgatga cttcacgacc481 agctcctgga gcccctcctt tctgctgaag ccgcggcatt tccctccgtg gccacacgag541 ggcacccttg gccttttatc aaagcgcctt cacttccccg tgggaatgga gacaagtctg601 tccacggtgt tttcttgana tacccagttg ctacccagat tgtattttta tgtaaccaat
661 acattttcac agacatacat tgacaaatac agtgaaacaa gaagaacata aaaaaaaaaa721 aagggggggg gggtaaaaat tccccagggg gccaattacc cacccttttt gaaagggcca781 tggggggata aa<210>20<211>536<212>DNA<213>Homo sapiens<400>201 tttgaagtca tttaagtttt tattaaatat actctcttgg cacaaatctt taaaatatat61 aaacattaga tataaacagt gtcttcatgg catcaaaata taactccaga ccaagaccag121 tgaccagcag ggaggcgagt gaccgcagag cctgagggcc tggctgtgtg caggggtggg181 ggacagcact gtccccaact cctgccgggt cccactcagc taggagccat ctccggggag241 acacactggg gtcagcaggg actgggggac cctcccacct accccttgtc tctcctgcct301 agcctgtggc cccaaatcca ctcatttggt ctcacccacc tggggcagac ccccaattcc361 tactgctgtt gcatgcttgt gggagttggg tgccctccca tctccctgtg agggttgccc421 tgcctctcct gccaagttta agtgctgggt aagggatacc cacacagtgg gacagcccct481 cacagccatc acaggctgcc tgggcctctg gggacaagat cagggatggc tggtgg<210>21<211>889<212>DNA<213>Homo sapiens<400>211 gaagaagcag aaggagcagg agcaagtgag agccacgagc caaggtgggg acttgatgtc61 ggatctcttc aacaagctgg tcatgaggcg caagggcatc tctgggaaag gacctggggc121 tggtgagggg cccggaggag cctttgcccg cgtgtcagac tccatccctc ctctgccgcc181 accgcagcag ccacaggcag aggaggacga ggacgactgg gaatcgtagg gggctccatg241 acaccttccc ccccagaccc agacttgggc cattgctctg acatggacac agacaggaca301 agctgctcag acctgcttcc ctgggatggg ggtgatggaa ccagcactgt gcggagacca361 gcttcaaggc agcggaaggc tggcctgagg ccacacagct ggggtgggga cttctgtctg421 cctgtgctcc atggggggac ggctccagcc agcctgcgcc actgtgtact tctctaaaga481 ggcttccaga gaaaatggca cacaatcaat aaagaactga gcaaacaaca aaacaacaac541 aaacaaagat agaaacgaaa aacacgaaag gggtggagcg aacaaacaga tcacgcgcag601 ggcaccacga cagaagtaca cgggaacaaa gaaaaagggg cctaacaacg cggccatatc661 agcacacgac aacgcgcgaa aaatacaaca cggaccacgg aacacgcgaa aaaggaacac721 tgcgacgacc acaccaccgg gcgagcaacc aaaggacgaa ccaccgaacc gaacacaacg781 ctaagagcaa acagcacctg acacgaggaa acggaacaca cagccccaac gcacccggcc841 acaagacagc ccaacacgca ccccggaccc ttcacggaag gtacgaaca
<210>22<211>880<212>DNA<213>Homo sapiens<400>221 gatcctcaag gcctgctctg ccccgtggag acgcttgctc ggatgagctc aggaaacagt61 accggctgcg tggcaggtct gggtgttgtg tgcgaggacg tggcctttga acaccgctgt121 gttctcagag gtccttagga gatatttttt tttgtcttag ggggactgtg ttaagttcag181 acaaatcatg ctgggtgtgg agagagtctg aaatacgtca gtgaagtaag tagcagtgag241 cgattgtgaa tgtgtaatgt aaatggaaaa ccgggtttta ccgtgttaag ttattcacta301 gggagccagt cgtagttctt tgtaatcctc tttcttccaa acctgctttg ctgaaagttg361 cagaaaagga agtgtgtgga gagaaacaga acccttcagg gtgggtcaga ggacgccatc421 cacagtggat tcgtgttcgt ttgcaggtgg aagcagtgat ttttaggacc cactgattaa481 aaacaaacat tcccaagtgt ctctgagaga tgctgtttat ttgttaatta aaaagctttt541 ttctctgtct tttaaattat ggtttcatgt aataaggata tttttagtga aaaatggttt601 tcctttcaaa ttacagacct tttaaaaaaa cttaatttga gcgagtacct tttcattgac661 actttcctgt ttctaacctt aggaacagaa tagcgttgga gacacgacgt ttcagttact721 ttgaaacctg ccactccatt ggttgtgatg ctcctttaca taatttctgg gacaaaaaaa781 aagggggggc taaataccgg gggccagttc gtccgtttgt aagggcccaa gggggtaaaa841 gaggggggta aaggggagaa gggcttggac ccccggggag<210>23<211>732<212>DNA<213>Homo sapiens<400>231 cggatgatga gatggaggag tccgacgtgg aggaggacct ccgcagactg acccccctca61 agcctgtgaa gaaaaagaag caccgctttg ggctacccgt atgacacatt cccatgctgg121 gggtgacggg agggccccgc cagccgctgg tgtgcagagg tcatcccaca gcatcgttcc181 ttaccctctc tctgcccttc ctttctacct ctgctcctct tgctgtctct gcccgctctc241 gcctgccccc agactactgt gacttaaaaa gagggaagag gagccagcgc ccgagggggc301 cactggcggc tggaggtccc cattcagttg cactacaaac actgaccaaa tatgcaagga361 aggagctgtg tttgtttgtt gtcgtcccag acagtgttgt gagggacctg aggccctgcc421 ccgtgtccga ccaccgagtg gcaaggtgga aggaagcaca ggcacacaga ccgtgggtgg481 gtctcctcac cgtggctgtg ggcagtgcga acacgtaaca ccctcgggct aaaagtgact541 cgttgaccaa gttggaaccg gaatgctttc ttactcaaaa tggcttttgt aactattgat601 ttctgaagct ggttttatga cgttgtgaca gtgttaccag gttgggggta tgtgtttatt661 tcctacaaag tacttacggg actaatgggc aaaacaggag atttttaagt cttccgtaac721 aaaaacaaaa aa
<210>24<211>628<212>DNA<213>Homo sapiens<400>241 gctttccgcc gcttgggcgc cttgtccgga gctgcggcct taggcttcgc ttcctacggg61 gcgcacggcg cccaattccc agatgcctac gggaaggagc tgtttgacaa ggccaacaaa121 caccacttct tacacagcct ggccctgtta ggggtgcccc attgcagaaa gccactctgg181 gctgggttat tgctagcttc cggaacgacc ttattctgca ccagctttta ctaccaggct241 ctgagtggag accccagcat ccagactttg gcccctgcgg gagggaccct gctactcttg301 ggctggcttg ccttggtctt tgagctccct tttgcttaat tactgggttt tctgggcagt361 ttttttttta aagagttgga gtaagaagag gattaaaaag gaaacggcaa attaacaaaa421 aaacaaaaaa aaaaaaactc tcggagggct tctatttgcg gggtggggga aaaaacggtg481 tcttgagatt tgggccttgg gtcatacgtg gttctcctgg acccggtttt tttgttacaa541 tgtgggaaat ctgcgagggt cccccctatt aggggggtcg gtattaattt cggataacgc601 cgggttaacc tggttttgtt ctctcaga<210>25<211>1245<212>DNA<213>Homo sapiens<400>251 agtgttctaa cccggacgtt gtaagaggga acttttggca ccttctttcc attatccatc61 acccccatca tgtcctccca aacacaaagc aatggcagga gaaaccacag gacagtcaca121 aagtcagtca cagagagatc tgtacaatcc cggatagctg gaattcaaga gtctgtccca181 gctatcttgt cctggagtgg ggttacagct caggggcata gggctcctca gtaggtgaag241 gtacccgcag ctcagcatca tgccttacca cggtgaagag tcccaccact gccagcagag301 ccatcaccat gacagcagag caaatgctga acatattccg agtgcctgtt tttcgatcac361 tgtcatggag gacaaggagc cctaggcaag ccagtgagtg cagaggtacc cggaaccagt421 tgagtacacc agcctgctct gtctcaggga tcacctttct ccgtaggaag ctcatgctgg481 gaaagtataa tccacaagcc aactcaataa gtagaaacgg ctatgaagga ctccaccgga541 ctctcctggc ctgggctggt agagaaagtc aacatgaaga gagagaagac gacgatgagc601 acagcaaggg acagcaggtg catgggctga aggtggtacc tcttggaggt ggcgatacgg661 tacaggcaag agccaagcag gctggctgcc atgaagctgg agaagataat gcccagaggg721 gccccgtgtg ggtccagcac aggtgtccag aggaagacaa cgatgcagat gacactctca781 actagagctt gtattggtgc caacagcagc acgcggcggt ccgacacgaa ggcacgcaag841 gctccacaca aggtccctga aaaggccctg ccgtcattat ttccccccgg atcgaagggc901 caggtccctt tcaaacctgg ctatgtggtg tgcccatagg cgcttagtcc caaacctatt961 catcagtgct tacggcctga tgggcaagcc gcggcaagag cgggcagtga ttccaagggg1021 cctaagccaa ggaagtggga gatcactaaa gaaacatttg catcagactc aggatattat
1081 cgcctgacga acaacagccg atgatgcaca gaattcctct ctactancag taatcgatac1141 tgacacacgc acgcattgcg aatagaaaga tacatacaca tcggacctct gattcataga1201 cgaaagaagg tcctacgtgc cggcatcaca gacngcacng agtat<210>26<211>655<212>DNA<213>Homo sapiens<400>261 ggggaaaaga ggggagaaaa acaggagtga tgtcatttct ttttcatgta ttccaattaa61 agaaacaagg gcaggtcgta taatggcata ttaatacatt agacttaatc tagaacccct121 gtagcttttt gatgtgttta tttcttatct ctttgaattc ctgtttggtt acttggcttc181 caatggaggt gaacttaaca accatacttg aatattccgt cttgatttgt aaactgtggc241 tacttgaaat gaagtttatc tggggttgat ggatgaatgg tagatttttg caatgtctca301 aggcaatagg atgtgtatta aacctgtaga tattcttagt acagtaaatt tatgctgata361 attttatttt gtataatttt tacctttttg ttaatatttt ttccttccac tttatggttt421 gcctcctgag ctacccctcc ttaccctccc ttctccctca gtgtttcagt aaatttaaat481 ttagggtgcc tagaaattgc aagtatgtat cctttttgat tgtgtatttt attataaatt541 tacacaaaca actgggtttg tgaactgtat tactcctggt atctttaaaa tattgtgggt601 gtcttaataa atttatatct atttttgcac tcaaaaaaaa aaaaaaaaaa aaatg<210>27<211>759<212>DNA<213>Homo sapiens<400>271 ggctctgcag gtggtccaca gccggcagct tagacaggcg cctgggcctc cagaggagtc61 ctagcacctg ctggccatga gggccacgcc agccactgcc ctcctcggcc agcagcaggt121 ctgtctcagc cgcatcccag ccaaactctg gaggtcacac tcgcctctcc ccaggtttca181 tgtctgaggc cctcaccaag tgtgagtgac agtataaaag attcactgtg gcatcgtttc241 cagaatgttc ttgctgtcgt tctgttgcag ctcttagtct gaggtcctct gacctctaga301 ctctgagctc actccagcct gtgaggagaa acggctccgc tgcgagctgg ctggtgcact361 cccaggctca ggctggggag ctgctgcgtc tgtggtcagg cctcctgctc ctgccaggga421 gcacgcgtgg tcttcgggtt gagctcggcc gtgcgtggag gtgcgcatgg ctgctcatgg481 tcccaacaca ggctactgtg agagccagca tccaacccca cgcttgcagt gactcagaat541 gataattatt atgactgttt atcgatgctt cccacagtgt ggtagaaagt cttgaattaa601 cactttggct tcaaaaaaaa aaaaaaaaaa aaatgacaaa aaaaaggggg ggtcttattg661 tgggggggaa aaaaaggaat aagggcgggt gaagttcggc acccttttga agggggagaa
721 tgggggccaa aaagggggga aatgaaaacg gccgaaaag<210>28<211>781<212>DNA<213>Homo sapiens<400>281 atataagtgt ctgctagatc atggttgtat gaactaacaa ggttgcagat ctgctgactt61 atataaagct tttgattcct actaagcttt aagatttaaa aaatgttcaa tgtgaaattt121 ctgtggggct ctattttgct tnggctttct ggtgagagag tgaggaagca ttctttcctt181 cactaagttg tctttctgtc ttctggatag atgattttaa gagactaagg gaatttacaa241 actaaagatt ttagtcatct ggtggaaaag gagactttaa gatgtttagg gctgggcggg301 gtgactcaca tctgtaatcc cagcactttg ggaggccaag gcaggcagaa cacttgaagg361 agttcaagac cagcgtggcc aacgtggtga aaccctgtct ctactaaaaa tacaaaaatg421 tttagctctg tttttcataa tagaaataga acaaggtaaa attgcttttc ttctgaaaaa481 gaacaagtat tgttcatcca agaagggttt tgtgactgaa tcagcagtgc tgcctagtca541 tagctgtgct tcaaaaacct cagcatgatt agtgtgggag caaacaggaa gcaagcaata601 cagttttgaa ttctatctgt ggttgactat ttgtatacta acttggtgtg gaatcccaaa661 aaaaaaaaaa aaaattgggg gcgggcctga aattttaacc tttggggggg gcctggggaa721 gggagtcagg acgggggagg gggttccaaa acgggcggtg aaaagggggg cggtaggtca781 c<210>29<211>437<212>DNA<213>Homo sapiens<400>291 tttttttttt tttttttcaa tgctgatcct gccactttac tgaaaatcac gttattaacg61 tttaaaaaac gtatgggaac catgcaaaga catccacgcc cctcagttgc tgccgctcca121 gcacctggct ccgaagtctg tgcagaaggc gaggctcccg tagtgctcga gagggccttg181 cacgctcccc gtggagggaa caaagctacg tggattttcc agcgcgccgc ccacttcacc241 aggcagcctc tgggcttcat cctgctcgcg tgacaggtgt gctctgtgct gtcaggaaac301 tgcagggctt tgagtataaa ctgaaaacag caaaactatg ttctcatctc agaggcccct361 tccttctcac cccgaggtca cctgggcccg gagacacagc acccgccgtg caggagcctc421 tgacccgcct cgtgccg<210>30
<211>572<212>DNA<213>Homo sapiens<400>301 ataaaaagaa ttatttaata ggaaaaatcg ttgttaaaat atggcaaatg gttatattta61 aaatgcaaat aactgataaa ctaaaaagat aacactcccc atctccccag tagataaatg121 gacgaaagaa cgaataacaa agggagaaat acagctagga aaaggaaaaa atgtttaagc181 tcactataag tgggggaaag gcaaattaaa atatattcaa atattatttt cacctcatca241 aaaatcattt taaaaatgtt ttcataccca attctcacaa gggcagcata aaaatggttc301 tcccctacat tgctggtgaa attatggttt agaacaaccc ttttgggaag caatttggca361 atataacaag aactttgcaa aagatttcta gattctcagt aatatgactc ctgggcatct421 gttcctaaag aaataaccca aaaatatagt tagaggtggg gaattaagag aactatataa481 ttttcaacat ttttggaaac attaattctg ttagccacac agggatcaat tttaaatatc541 caaaagttgg accatgacag aactatccaa tg<210>31<211>472<212>DNA<213>Homo sapiens<400>311 actctattga aaattttatt aaaatccttc atttatcttc cagatagtag attctattaa61 ccaagttgca taaagagttc tgattcccag aggtaagaac tgagcctggc tttctagtta121 catgtttact ggtaaccccg tctttcccta taacacttaa gcatatttaa agaatatacc181 ctcccctgcc tccagccagg attgtaagct cctcaagagc atggctgtgt cttttaacat241 caaggctcca ggtgagaaga gttcctgctg tattataagt gcccaatatg catttgtaag301 tgaagaaatc tattctgtgg tgtctgcttt aatattaaga gatggcttgg cagagattac361 taagcagctt cattgtctaa aatggaacta gattctgtgg gtagcaagtc cattggtgcc421 actcacagga ggcctcaaag aaaccaaact gccccacaaa tctgcgggag tc<210>32<211>762<212>DNA<213>Homo sapiens<400>321 gggcaagccg aatcctagag gctaacgcgg caggtgggag ggagaaagtt gctttctgca61 ccaatagctg aggcgttcag ggttgtccag gacgctaccc tcacatgtct ggttccgagt121 gctgcgttcg gctgtgctgg gaagttgcgt agacagtggc ctcgagaccc tgcctgcctg
181 aggaggcctc ggttggatgc gaaggagctg cagcatccag tgcctctcca tgagaccata241 agctcctgta tgacagaagc catgttcttt ttagcggcct ccgtagcatg atgggacaag301 atgccaactg gcaagcagct agctgacatt ggctataaga ccttctctac ctccatgatg361 cttctcactg tgtatggggg gtacctctgc agtgtccgag tctaccacta tttccagtgg421 cgcagggccc agcgccaggc cgcagaagaa cagaagacct caggaatcat gtagaactgg481 ggggcttttt ctcctgagca gagaggccca aggcatgctg tggagagact tcacctgcca541 ccatttccag gtcaacagga ctagagcgtt gatggttttc aaaccctgtt ggaagaaagt601 gccatggttt ctctggttct gcagtttgac agtttatgga ggctttgaat cgtaatagca661 atgtgagggt gaggtacacc tacagacatt aattatttgt gtgaaaaaaa aaacaactaa721 tatagcaaaa aaaaatgttt tttggggcga aaaatttcgc cg<210>33<211>632<212>DNA<213>Homo sapiens<400>331 gcgatctaga actagtgttg cgggaagctc ccgggggtcg cacgtgcgtc cgagcccaag61 cccctcccct ccactcccct tcctgcgtgc cccggagccg ccaagcggct acgttcttct121 cggcccgccg agatggcgct cgaccccgca gaccagcatc tcagacatgt cgaaaaagat181 gttttgatcc ctaaaataat gagagaaaag gccaaagaga ggtgttctga acaagttcaa241 gattttacca aatgttgcaa gaactctgga gttcttatgg tagtaaaatg ccggaaagaa301 aattctgcat tgaaagaatg tctaactgct tactataatg atccagcctt ttatgaagaa361 tgcaaaatgg aatacctgaa ggaaagggaa gaattcagaa aaactggaat tcctacaaag421 aaaaggctac agaagcttcc aacaagcatg taggcagata ctcaaatgac attcaggaac481 tctaatattc atggaagtca ttttatagtc cttaaataat ggactcaagc atatatgttt541 gctttacctt aattatggaa atattaactt tatctgaaat acatattttt atttcaaaaa601 aaaaaccaca acaaaaggga gggcgcgctc gc<210>34<211>834<212>DNA<213>Homo sapiens<400>341 ctgctgcggc ggggcctgga gctgctggcg gcgtccgagg ccccccggga ccctccaggt61 caggccaagc cgagaggggc tccggtgaaa cggccccgga agacgaaggc aattcaggcc121 cagaaactgc ggaactcggc caagggaaag gtgcccaagt cggcactgga cgagtaccgg181 aagcgagagt gtcgagacca cctcagagta aacctgaagt ttctgaccag gacgagaagc241 accgtggctg agtctgtgag ccagcagatt ttgcgccaga accggggccg caaggcctgt
301 gaccggcctg tggccaagac caagaagaag aaggctgagg gcaccgtgtt caccgaggaa361 gacttccaga agttccagca ggaatacttc ggcagctagg ctccctggag ggcacggtga421 agaggccttc aagccctgca gcctccgact cctgctggct ccaggaaccg gccgtgccgc481 gcggcagcag atggcgatgc aggaccagcc tggctcgagg aagccgcgga gctgagccga541 gtggaggctg gaatggagct ggtgggccgg aagtcctggg ggaggattta cacacagacc601 ggagctggct tccgcaggcc tgggcagagc atctgcacct gccggaaagg aacgtatctg661 tctgttgttt tggccagtgg ggctctgggc tgttgtgtgg agcaggtaat ctgagcctgg721 gcgacgaaag tcaggagtag agaaggtggg ctccaggtac aataatgtga ccagaaaaaa781 caaaaaacac ccacaacaac acaaaaaaga gggggcaaaa acccggggcc aagc<210>35<211>503<212>DNA<213>Homo sapiens<400>351 gtcagtagaa ggtgatttat tggaaagttt agaaacatgt ggcaacaaga caggtagatc61 tctgcactgt tactccccag accgagagct tacataccat atagaaagag cataagtgct121 tcagaaggaa tgtgtaggat cgctgaaatt gacccctcag ggaaaggcaa gaatgctgtg181 tctcatagcc cataatttgc caaaggacag gacttaggta agtacatgtt tatgataaca241 tcaaggttgt cttggcttac acaagcaaca gtaagtaaag tagaaatctt gaggcattcc301 tgggcctggg gttaatcaga agtcaacatg gcagattagc atccaagatg aagtggcatc361 atcctgcaca gtgtccaacg ttgtacaagt gtctttaaga tgcagaagta gggttgaaac421 cacactgaag taggtgacac tctcttcttc cttatccggg aatgagttgg ttgttggtga481 tcatcagtct gatccactcc aag<210>36<211>1081<212>DNA<213>Homo sapiens<400>361 gccgcggagc ggaacaagga tcccatcttg cacgtgctgc ggcagtacct ggatccggcc61 cagcgtggcg tccgcgtcct cgaggtggcc tcgggctccg gccagcacgc agcgcacttc121 gcgcgggcct tccccctggc cgagtggcag ccgtcggacg tggaccagcg ctgcctggac181 agcatcgcgg ccaccacgca agcccagggc ctgaccaacg tgaaggcccc gctacacctg241 gacgtgacgt ggggctggga gcactggggc gggatcctgc cacagtcgct ggacctgttg301 ctctgcatca acatggccca tgtcagcccc ctgcgctgca cggaggggct cttcagagca361 gcaggacacc tgctcaaacc cagggccctg ctcatcacct acggggaacc cagaatgggg421 gcttcgggac acagccctcc tggaggacct gggaaaggcc agtggcctgc tcctggagag
481 gatggtggac atgccagcca acaacaaatg cctgatcttc cggaaaaact aagcccctcc541 ttcagccccg cacacctgca tccctgccgg aggctctgtg aggcacgaac cctgactccc601 taggccggac cttgtggacg acagcccacc cagtctgtgc tctcagccgc tggccgaagg661 gcccaggctg ctcagaataa agcatgtctg ctgccggaaa caggcgaaaa acctcggagg721 cacaatgcag ggccgggaaa acgaggtacg aatgggcatg ggcaaagcgg gtcccgggac781 ccaggttcct ggtcaccagg ggtaaacttg cgggccccca aaaggagccg gaataatccg841 gaaaggcagg gtaacgtaca acacccggcg agaagaacgc agcgaacacg gcgcggacga901 gcagcagaga aagcaagagc aaagcccaca cacgcagaag aacagaggac aaaagcgacc961 accagcagac ccagcagcgg gacgacgaaa gacgagagca caggacacaa cagaacgaaa1021 agacacacgc gcagccaaca cgacagaaca gaagagagaa cacacacaca agagcaacac1081 a<210>37<211>893<212>DNA<213>Homo sapiens<400>371 cactcggcga tccccgactc ccttctctat ggcgtgctcc tgtgctgtgg gctcgaagct61 ggccgcctgg gcatcgtcct cagcgcctgg ggagtgatca tgttgataat gctcggaata121 tttttcaatg tccattccgc tgtgtgattg aggacgttcc cttcacggag aaagattttg181 agaatggccc ccagaacata tacaaccttt acgagcaagt cagctacaag ctgtttcatc241 gctgcaggcc tttacctcct cctcggaggc ttctctttct gccaagttcg gctcaataag301 cgcaaggaat acatggtgcg ctagggcccc ggcgcgtttc cccgctccag cccctcctct361 atttaaagac tccctgcacc gtgtcaccca ggtcgcgtcc cacccttgcc ggcgccctct421 gtgggactgg gtttcccggg cgagagactg aatcccttct cccatctctg gcatccggcc481 cccgtggaga gggctgaggc tggggggctg taccgtctct caacccttcg ctgtgtcccg541 tatctcaata aagagaatct gctctcttca ataaacagaa ccgaatacaa cacacacaag601 aacaaccctc aagaaacaag acagtccccg cgccacacat gttggaccgc cgcggcccca661 aacagctcac gtaattacgc gccgcccgat gctctctgct tcagacgatg gccccctatc721 tattctacaa gagcgggcaa tagtcccgcg ccccaccgga ggcggtcgcc tatccgtgca781 aaccccacca atcgtacata gacgcgacca ccgccctcac aatacactgc agacaagagc841 ccccaccccc cctcccaccc ctccgcatac accagatccc cctccaggcc gcg<210>38<211>581<212>DNA<213>Homo sapiens<400>38
1 aatctttttt tttttttttt tttttttttt aaaatggact tgaattttaa tttttttaaa61 gattatctat atatatatta ataaaaagtg caaagaatag accttcatgt ttaaattata121 ttctaaataa aatatatata tatatgtata tatatttcca ttaactgcgg gagacaactg181 gcaagtaaag agcctgagag agtccagtcc ctctccacca ctacaatgga catatatttc241 aaggaagcac ctatgaaaaa tagtagagtg ttcacaacac atctggtaac acagatagct301 tctgggtgtt cttgagagca gtttgtttgc attttcaata tgacatgaaa tgaagggaaa361 aatcaaattc acaaaccgaa atgacattac ttagaacaat ctagtattct gtattcatgt421 tttacatgcc ctagagccta gcattttcat gtagcaagct acactcacat gtgcgttaag481 ccgcgtatct caaatggtga tcagctctta gtgcaagtgc tctctgctcc taccactgct541 ggttgagtaa catagctgga acatctggtt agaactgaat t<210>39<211>1003<212>DNA<213>Homo sapiens<400>391 taagtaaaca gacattggag aaaatatact gggagttgtt aacagatgac ggcaatattg61 atgaggctgt tcaagtgttt tacataaggt aataagaggt accaccatca ccagaacccc121 tacaaatttt tacatttcat ataaaaagag taaaaatagt tcattggcat gtaactagca181 tcattctctg taatctatga gccataataa gcctgaacaa tttcagtctc ttcagagcaa241 aacaaagtat tttattccac tagcagcatg tttctccaaa aacaaaaaac aaaccaatac301 aaccacaaat actacccaca cacaaccctg aaacataagt tgccatattc cagtggtcct361 gaattttaac atgttttgct ctacattaat tcaagaaata aaatgagaaa cagctttgaa421 aatgagatta actcctttgc tgtaattata cttactctat aattcaagac tatttagctg481 aagtcagtta acgagtaaaa accgcggata cagctcaaac tgctcttaac ttctttaaag541 tgtttactgt tctattcaaa actcagaccc aagctgcaca ggtgtaactt gaggcactaa601 caatcttcct accagacgta atagttttat gtgttcttaa agctgggcgc atatacaaaa661 tcactgtaca tcacacataa acatcagaaa cttttcttgg ccacaactag accaaatagt721 ttcctctatt attaatgtat gttgaaatca cgcatgtgtt accagttcag catttaaaag781 aactccatgt tatgatttaa aaggatgctc aggttgagaa ataccttgag tactggcaaa841 gaaccgccaa aaggaaacag attgaatggg ggtttactgt gaaccttagg agacccggtt901 tcaacaaaaa gcaccaaaac ggtgactgga ttagttaagc aagttccccc tacacactaa961 tagggtttac ccctggtttc caaaagttaa aggggtctca agt<210>40<211>551<212>DNA<213>Homo sapiens
<400>401 ttaaagtatt ctttattaag atcattaaaa gttgtagaaa caactttata ttataaaggc61 acagggagac agttcaataa aatattcaaa ttaacagtaa cttacagtac aaaagacaat121 tttaatgcac atacaaaccc caaatgtctg attcaaaaat attttactgc gtctattaat181 gggcaatgat taaaaatata aattgctgcc atataataaa tatctgatag tgaacatctt241 gatagcaaag tgaacaaaat atttattgct tttcacttac atatttttcc ctgtaatttt301 aaattcctaa ttcttttact atcttctcaa cttttcccaa agataaaata aatttcacat361 aatttcatgg aggggaaatg gtagttgtaa aaaactacct caagtagcaa tcaccgctgg421 cagtgttttc tcactttctg ttctgcaatt gcaatcacac ttccaaaaag aaaagcaaat481 gtttgctaaa ccatagacag acaacctctt tgtgactggt attataaggt ttataatgaa541 aacttatcaa a<210>41<211>722<212>DNA<213>Homo sapiens<400>411 gagggagggc tatagcttta gcaatgttga tgagccacat ttcccacaca gatctgtagc61 attgctgttg acctctttga cgatgaactc tcccataggc cctgccaggc agcactgaga121 catggaggaa attcagacaa aaaatggctc accctgatag ggaaggagtg actatatttt181 aaattaaact gctttttcca acactgtgag gtttctgtaa tatttagctt ttatttggaa241 gcgatagcgt atggcatttt ttatgctgtt tggtttatat tgtctactgc aggcttcttt301 gtataagctt gcctgggctc accctctcct ggacactgtt ttaaagtgtc accgctgtcc361 atgctgccag aggctctgat gtgaagctgt actctcaaga tccacgtttt taagtcggca421 tgattagtaa tgactttttg tggcaataga aaagcccatt aactcaaaaa aaccaaggag481 agtaaaaagg aaaggaaact atgaatcgtg cctcatcttt cattgaatcc tatatttatt541 attctagaaa aaaagaactt acaagataga gaagaactaa tagagcatct actttacccc601 tcaggctgtc cagtggggat aattattaat aaacatttaa tggacaattc aagatcttcc661 aatttaattt ttaaaaatag tttataccaa atattataag gattctttca gccgattagg721 ac<210>42<211>478<212>DNA<213>Homo sapiens<400>421 tttggttgta aaattttgag atagttttta tttgaaaaca taacattaca aagtttgaaa61 aataaaaaaa ttaatgattt taactatttt tttcttttgt tctcttgttt ttaacttact
121 attttgaatg acaaaaaaat ggctcatatg tttgtccaaa acccttgata atatctttaa181 ttttttaaat acctgaaaca gttaaattat tatgtactaa aaacatttac tgtactctca241 gacagactca tataaaataa cctcatttgt caggagaaag aaaggtacat acaaagtagt301 aaattatgag ttttgacaaa agtaatagac atttttagtt atttaaataa attaagaaaa361 catcccaaaa gacatttata cagtaaaaaa aaaagtaaat aatttcagga aaaataaact421 gtattattac atttaaataa acatgaaaaa gatacttgga tgatggtttt aatttctc<210>43<211>499<212>DNA<213>Homo sapiens<400>431 tgtagctgtg cccttatttt tatagatagt ttgccatttc ttgggtttaa tgtttaacaa61 ggctatagaa gtttaaaata gtggacaaat gtttgtgaat acacttttaa aaaattgttt121 taaaagagaa aagcggtctt gctttgttgc ccagactgga gtgcagtggc ataatcatag181 ctcaccgcaa gcctcaaatt tttgtgctca gatgatcctt ttcatctcag cctcctgatt241 agctgggact ataggcgtgt gccaccatgc ccagctaatt ttgaaatttg cttgtagaga301 tgggatctca ctctgttgtc caggctggtc tcagaattct ggtgagtgat cctcccacag361 tggacttcca aagtgctggg attacaggcg tgagccacca tgcctggccc catgaataca421 ctactaatat tatttgtaaa tatgtatttt ataatgtaca atgcttggaa aattttcaga481 gtattctagt ttaaaaaat<210>44<211>568<212>DNA<213>Homo sapiens<400>441 tttaagtttg aagccttgcc caagctttaa tgtcatgcta accagttacc ttgttagagc61 tgggaagcca ccttttgctc aaaatgcaga cttctgcctt tgaaaacaca ccacacctga121 ttttaagtgc ttaaaggaca gaaaatgtcg ttgctttaaa ttgttgcttt gttcagagac181 atctggattt gctgtatcca tacaagcaaa agcttttcca attccagaat caacccacac241 taatttgtta ttgcctcgta ctgtattggc ccagctgtaa tcaactcagc aggcttttgg301 ttgtaaattc aagtggaaat tgagttggtc ttatttgtgc ccgttgatat ctgagggctg361 tgaggaagat ggccagtagg tcaaagggaa tagtgttgaa aagctgacca gtactgggca421 ggtggccggc tgtccctcat gcaccacaac gccgagccac ggtaaggggc atgagccaca481 tttgcagaat atagccagag tccttccaga acctcctgat tcgcgccagg aggcatccca541 nggcacacaa gtgtcaaggg cctactca
<210>45<211>541<212>DNA<213>Homo sapiens<400>451 ggtgaccacg tcctccggct ggcgcagcag caggaagagc aggaagtcgg agatgagcgc61 gtgggcttcg gggtgctgcc gcagatagct ggcgtggccg agccggagct cctcctggca121 ggggacagag cccgagcctc agccggacgg gacccccgcg ccccaggagc ccccagccgc181 tcgctccaag gagagctaag gggctctgga gccttctagc ccctacgggt gtgcatttca241 atcaggtgcc tggagtgcaa cacaacatgt gagagtgaga atcttcccag accgcctgct301 atggactgaa ttgtctctct aacccccagt gtggtggtat ttggagaaag gccatgtgag361 gacacattga gaaggcactt gtctgcaagc taggaagaca gccctcacca gaaaccaacc421 ctgatggcac catgatcttg gacttcctgc cttccagcaa aatatatttc tcttgaagcc481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa541 a<210>46<211>1452<212>DNA<213>Homo sapiens<400>461 atgcccagaa aatatggtaa gctatgttaa caagccatat tagatatcaa gcattaatat61 ggaaataaac cagcccacct gcacccaggt gaaataaaca gctttattgc tcacacaaag121 cctgtttggt ggtctcttca catggacgcg catgaaattt ggtgccgtga ctcggatcgg181 gggacctccc ttgggagatc aatcccctgt cctcctgctc tttgctccgt gagaaacatc241 cacctatggc ctcaggtcct cagaccgacc agcccaagaa acatctcacc aatttcaaat301 ccggcattgg gctctcacca ttggccaaga ttccctgccc cggtgaggcc ggaagcagtt361 cagacacctg acttctggca acatgtgagc ctgacccaag cagatcactg gtgaccagta421 aatattgaag ctccaagagt attttccaaa agaagagcaa gggcccggct ggaggctgac481 aggcttccaa gaacctgaat cccctcttgg actcctggct tgcttttggc tacagaatca541 tcatctgcat ctggagattt ccactgggac tttctggagg cagacaaatc ctgccctgat601 tctggggacc atgcctgtgg agatgcacag ttctaccatt caaaatgact atctgaaaat661 acctcctaag aaggacaaaa cttttcttca gccaagaggg cacttgttaa tatgattaga721 tacatatgta atggtgtccc cctcccctct tagcccctca gtgtgtgttc tctcagtcag781 tgtatctaca tgcaggagac attaaaggat ggatgtaact tcagaccttc atttccccat841 aagtattgct ttcagagaat atctaatgaa gtcacatgga tgaagatttc tgtatcattt901 gtttcccaga atggctcccc aacttacatt tcatcatcca cccacatatc cacttatcca961 ttcaaccttc atgtactgac tgaaaactcc tcttatatag ttttcctcag cagtttcctc1021 ttctggggat tttctgctgt tttggtaacc tgttttaatg ttgtatttta tgtgttccta1081 agggcaatgc aagttgttta aagaggcaat tatctcatgt gatttatgag atttgctgga
1141 caaattccaa taaaaaatgt actcgagaag tatgtcacag gtttattgct ggtatcaata1201 aagtctctat agtgtgtggc aatccatgtt ttaaaaggag ttagctttgt gatcatagat1261 tatcaaatct aatatgccac caattataag atttattcca gtttttgaga tgtaaaaatg1321 taaaaaataa aggtcagcaa ttattttata atttcatgta ttgtaagata tatgtagaat1381 caaaagacgg taaaatgtga aataaagtgc acctaagaat tgataaaaaa aaaaaaaaaa1441 aaaaaaaaaa aa<210>47<211>1201<212>DNA<213>Homo sapiens<400>471 ggggagagcc taccctattt attcccagca tggacctaaa tccacttcag ccaaaaatga61 acaaagaaat gatgaagaac agagcctgtt tctttctcag tggtgtactc agaaaatgga121 tgcaagagta gaagctgatc ctcgaggctt gcaaaagtgg ggttctttct gaaagtgaag181 ccagcagcca agagccaccc ttcagaagat agaaggaaga gcagctgcat gagtgatggc241 cacagcaatg catgggtcct gtctgtccac gtagtcctct ccatctgccc gtagcttgcc301 aggaaacttt ctataataat accaatacaa ccaaagacta ttattctgaa cagaaaacaa361 gaaatacagc tggagctatt tcctatctac ctgatgaagg gcactaccag gcggtcactc421 tggctctgtc caccgatgcc tgtgcccact ttgtgcgacg tagcttccat ggagacaaga481 gcaatggtaa tggaagaaga aagtctggat gtactcttgc agaagcagaa agcttcatgg541 aacaatatta atcttctggt catctctgtg ctggtctagt tgcccataaa gttgtcagat601 tgactttgga tttaaagcct gaaaatgagt cccaaggaaa tccgttgaca tgaggcccaa661 ggaagacagt ttttggaagg ggcaagcata atgatttgga atgatgccta ataaaataaa721 tggttgacca gaagattaat aatgtgttgg tttctcaata gaagaggagg aaaacatgtt781 tttaagattc ttaccaataa ggctcactcc acccagtcct gtgactcatg actcaccccc841 aatcaagaga tggactcagc tcatgaggac cattttccat acccctatga ctgcctccgc901 aaccaaccag caacacccat cacctagtca cctgcccacc atactatcct tgaaaatctc961 taatcttcaa gccttcaggg agactgattt gaaacaaaag ggaccctaga aatcttcagc1021 catccaaaag aaggcaaaaa aaaagagtat caaggaaaga ggatggcaaa cagaaaatgt1081 aaaagaagat ggtagaaatc acttcaagtt ggtaatcaca atatatgtaa atatattaaa1141 cacatccatt aaaaggctat gtgattagac taaaaaaaaa aaaaaaaaaa aaaaaaaaaa1201 a<210>48<211>554<212>DNA<213>Homo sapiens
<400>481 cgcgaaagga aagaggtcgg gagcgctcgc gagatctcgg accacccaac ctgaaaggtg61 cttaggaagt tgaaaggccc agaggaggcc tccgggcaaa tggccggagc tggaccgacc121 atgctgctac gagaagagaa tggctgttgc agtcggcgtc agagcagctc cagtgctggg181 gattcggacg gagagcgcga ggactcggcg gctgagcgcg cccgacagca gctagaggcg241 ctgctcaaca agactatgcg cattcgcatg acagatggac ggacactggt cggctgcttc301 ctctgcactg accgtgactg caatgtcatc ctgggctcgg cgcaggagtt cctcaagccg361 tcggattcct tctctgccgg ggagccccgt gtgctgggcc tggccatggt acccggacac421 cacatcgttt ccattgaggt gcagagggag agtctgaccg ggcctccgta tctctgacca481 cgatggcgct tacctttcag acttcattaa acttatgacc gaaaaaaaaa aaaaaaaaaa541 aaaaaaaaaa aaaa<210>49<211>883<212>DNA<213>Homo sapiens<400>491 gcacgtcggc cctgcctccc ggaccaccct gcggggcgca ccaccgatct ggggtgcggg61 gctcggcctc cctgcgctcc tggctgacgg tgtgaccttg ggaccgagac gtgcagctgt121 ccaaggctct gtcctatgcc ctgcgccatg gggccttgaa gctggggctt cccatgggag181 ctgatggctt cgtgcccctg ggcaccctcc tgcagttgcc ccagttccgc ggcttctctg241 ctgaagatgt gcagcgcgtg gtggacacca ataggaagca gcggttcgcc ctgcagctgg301 gcgatcccag cactggcctt ctcatccggg ccaaccaggg ccattccctg caggtaccta361 agttggagct gatgcccctg gagacaccgc aggccctgcc cccgatgcta gtccatggta421 cattctggaa gcactggcca tccatcctac tcaaaggcct gtcctgccag ggaaggacgc481 acattcacct ggccccagga ctgcctggag accccggtat catcagtggc atgcggtccc541 attgtgaaat agctgtgttc atcgatggac ccctggctct ggcagatgga atacccttct601 tccgctctgc caatggggtg attctgactc cagggaatac tgatggcttc ctccttccca661 agtacttcaa ggaggccctg cagctacgcc ctacccgaaa gcccctttcc ttggctggtg721 atgaagagac agagtgtcag agtagcccca agcacagctc cagagaaagg aggaggatcc781 aacaataaaa tattaattta taaaaaagaa attttaaaaa gtaacaagaa agaactcgtt841 tgaaaccatg tttcatcatc ctgtaaaaaa aaaaaaaaaa aaa<210>50<211>740<212>DNA<213>Homo sapiens<400>50
1 ctgctggtta agatccgtta tagtgctttg tttatttaga gtgcccttac ttagtggcct61 ctttaattct ccacggtgct atgagaacac cataggaagt agggttgaaa tagggccagg121 gcgcaccacg ggtggtggtg aggtcactgt ccaggtctgg gactcagcca ttgtgggagg181 gggacatgaa cagacactct accacatcag ggaatttgaa tactgtcttt ggatatgttg241 atgaagagac tgtcagccag ggcatttcgg cttcctatgt gggttctgga aagcttgttg301 gaaacagact cgtgagacac actcaaacca gtttgttttc ttcagcaaac acctggtaga361 gattggaatt gaacagtgag tgaagaatga cctcacgctg cccagaattc tgattccatg421 tgtaatgcga attctgagag gcctctgttt ctcagctttt gggtggtatt cagagaggtc481 tagtaacact tggtgtcacc tggctggtga gggcaggctc cggtgaccgc aaagctaagt541 gctctgtagg gcacctccct ctctttcagg ggctgcctgg gcctctggtg cacatgatcc601 atggagtcag agtcagagct ctcgtctgac aagtgctttt gctttctttc tttctttctt661 tcttttttgg agacagaatc tcactccagc ctgggcaaca gaatgagacc cttccgcaaa721 aaaaaaaaaa aaaaaaaaaa
权利要求
1.一类人类肝脏表达序列标签的序列，其包括(a)SEQ ID No.1～SEQ ID No.50所示的序列；(b)SEQ ID No.1～SEQ ID No.50所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.50所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
2.根据权利要求1所述的一类人类肝脏表达序列标签的序列，其特征在于所述序列包括具有SEQ ID No.1～SEQ ID No.50所示的序列。
3.一种探针分子，其特征在于所述的探针分子含有权利要求1中所述的序列中约8-100个连续的核苷酸。
全文摘要
本发明公开了一类人类肝脏表达序列标签。利用本发明的在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物中发挥重要作用。
文档编号C12Q1/68GK1955292SQ20051003082
公开日2007年5月2日 申请日期2005年10月28日 优先权日2005年10月28日
发明者黄健, 韩泽广 申请人:上海人类基因组研究中心