人类肝脏中表达的表达序列标签i组的制作方法

专利名称：人类肝脏中表达的表达序列标签i组的制作方法
技术领域：
本发明涉及生物技术领域，尤其涉及一类在人类肝脏中表达的表达序列标签。
背景技术：
肝脏是人体内最大的消化腺。也是体内新陈代谢的中心站。据估计，在肝脏中发生的化学反应有500种以上，实验证明，动物在完全摘除肝脏后即使给予相应的治疗，最多也只能生存50多个小时。这说明肝脏是维持生命活动的一个必不可少的重要器官。肝脏的血流量极为丰富，约占心输出量的1/4。每分钟进入肝脏的血流量为1000-1200ml。肝脏的主要功能是进行糖的分解、贮存糖原；参与蛋白质、脂肪、维生素、激素的代谢；解毒；分泌胆汁；吞噬、防御机能；制造凝血因子；调节血容量及水电解质平衡；产生热量等。在胚胎时期肝脏还有造血功能。
肝脏疫病分为肝炎、肝硬化、脂肪肝、肝癌等。现代医学实验证明，肝病病毒侵入人体后，并不直接引起肝细胞的损害，只是在肝细胞内吸收营养赖以生存，并在肝细胞内复制、繁殖。其复制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)释放在肝细胞膜上，引起人体免疫系统对这些抗原物质产生免疫反应，这种反应造成肝细胞的损伤、坏死。免疫反应的强弱决定于肝脏受损程度及临床症状轻重。这场由病毒引发的、免疫系统对肝细胞的战争，使大约25％的患者的肝脏成为战火连绵的战场，肝脏的损伤由此加重。肝病的危害绝不仅仅限于肝脏本身，它还可以引起其它多种疾病。常见的有(1)糖尿病；(2)胰腺炎；(3)胆道感染；(4)功能性肾衰竭；(5)胆汗性肾病；(6)肾小球肾炎；(7)肾小管酸中毒；(8)溶血性贫血；(9)再生障碍性贫血；(10)心肌炎和心包炎；(11)结节性动脉炎；(12)消化性溃疡；(13)自发性腹膜炎；(14)性激素代谢紊乱；(15)甲状腺功能改变；(16)肝性骨病，等等。肝病不仅对患者的身体甚至生命造成危害，而且对患者心理上的打击也是十分沉重的。无论是肝病患者还是病毒携带者，在生活、社交、求职、升学等方面都会受到严重影响。
生物基因组中可转录表达的序列(即基因)仅占总序列的3-5％，对这部分序列进行测定，将直接导致新基因的发现，并获取基因组中与产业化关系最为密切的信息。20世纪80年代，高通量的自动测序的出现，使从质粒互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库随机选取许多cDNA克隆和决定来自非载体两端的几百个碱基的DNA序列成为可能。这些短的DNA序列叫做“表达序列标签”(Expressed Sequence Tags，简称ESTs)。表达序列标签的概念最早是由Adams等在1992年提出来的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics.2，173-179)针对获得大量信使核糖核酸(mRNA)序列的迫切需要，提出大规模互补脱氧核糖核酸(cDNA)测序的研究战略。随后Venter创立了大规模表达序列标签技术。其基本特征就是从以质粒为载体，构建完成的目的组织互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库中，随机选择许多cDNA克隆，利用质粒上携带的通用引物对cDNA两端进行一轮脱氧核糖核酸序列测定，所获得的来自3’端或5’端的几百个碱基的非载体短脱氧核糖核酸(DNA)序列。简而言之，表达序列标签是来自表达基因片段3’端或5’端的短脱氧核糖核酸序列，代表一个表达基因的部分转录片段。
表达序列标签可用于新基因克隆、人类基因组图谱绘制、基因组序列编码区的确定等。如果一个表达序列标签在基因组中只出现一次，那么它可以作为序列标签位点(STS)。由表达序列标签构建的物理图谱叫表达图或转录图(expression ortranscript map)。利用表达序列标签进行基因图制作，可以加快序列标签位点的制作和新基因的染色体定位。表达序列标签可以作为基因特异性探针，对组织特异性基因表达的研究具有重要的作用。表达序列标签还可以进行新基因的遗传进化关系分析。表达序列标签可以对所有动植物的基因作为一种数据库，通过不同的序列比较可以获得保守序列片段，从而获得基因的遗传进化图谱。正因为表达序列标签具有如此的优越性，因此表达序列标签测序已经成为许多基因组研究机构的工作重点。
表达序列标签(EST)是一种快速有效揭示基因组容量的方法。ESTs在新基因的发现、基因作图和基因组序列编码区域的确定等方面起到重要作用。EST不仅为基因组遗传图谱的构建提供了大量的分子标记，而且来自不同组织和器官的EST也为基因的功能研究提供了有价值的信息。此外，EST计划还为基因的鉴定提供了候选基因(candidantes)。
在本发明之前，还没有出现涉及本发明的一类在人类肝脏中表达的表达序列标签的公开报道。

发明内容
本发明要解决的技术问题是提供一类在人类肝脏中表达的表达序列标签。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明的一个方面，提供了一类在人类肝脏中表达的表达序列标签的序列，其包括(a)SEQ ID No.1～SEQ ID No.58所示的序列；(b)SEQ ID No.1～SEQ ID No.58所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.58所示的序列中每条序列有至少70％同源性的序列；(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.58所示的序列。
本发明还提供了一种探针分子，所述的探针分子含有上述序列中约8-100个连续的核苷酸。
由本发明的一类在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中特异表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物中发挥重要作用。
具体实施例方式
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不是限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件如Sambrook等人，分子克隆实验室手册(New YorkCold Spring HarborLaboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1人肝脏组织的mRNA的分离组织分离(Tissue isolation)肝脏来源于5个成年男性，在肝脏切除手术后，将肝脏组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation)取出肝脏组织，用研钵研碎，加入盛有裂解液的50ml管，充分振荡后，再移入玻璃匀浆器内，匀浆后移至50ml新管，抽提总RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligod(T)的纤维素柱分离总RNA中的mRNA，定量。
实施例2cDNA文库的构建(Constuction of cDNA library)以mRNA为模板，合成双链cDNA。补平末端后，加含EcoRI切点的接头。磷酸化EcoRI末端后，用XhoI限制性内切酶消化1.5小时，再进行片断分离。过柱筛选长度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，无菌水溶解，连接至Uni-ZAP XR载体(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)进行包装，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)细菌。涂板并测定滴度。
实施例3测序及数据库建立(Seqencing and Database Constructing)挑选文库中有外源片段插入的克隆，扩增后抽提质粒(Qiagen Germany)，用T3和T7作为3’和5’端的通用引物，采用终止物荧光标记(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377测序仪(Perkin-Elmer，USA)上进行EST大规模测序。测序结果用FACTURA软件去除载体序列，传输到SUN Ultra 450Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group，USA)中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL)，将无同源性或同源性低于95％的序列视为新基因建立数据库。
实施例4基因的全长克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基础上，进行cDNA全长克隆，分两阶段进行(1)“电子克隆”(Electronic Cloning)以新基因片段序列作为探针搜寻dbEST数据库，将重叠序列＞50bp，同源性在98％以上的表达序列标签(Expressed Sequence Tag，简称“EST”)序列认为同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER软件进行连接，部分EST可以延伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(OpenReading Frame，ORF)，用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸和氨基酸水平上是否与其他物种有同源性，以帮助判别所得到的基因全长完整性如何。通过电子克隆的方法，通常可获取人肝脏相关基因的全长序列。
(2)cDNA末端快速扩增(Rapid Amplification of cDNA Ends，RACE)如果通过“电子克隆”方法仍未得到完整的cDNA全长，则在已有序列5’或3’端设计引物，在人类肝脏Marathon-Ready cDNA文库(Clontech Lab，Inc，USA)中进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软件分析被延长的序列有无完整的ORF，如无，重复上述过程直至获得全长。
(3)RT-PCR对于5’和3’端的已知的序列，如果中间有一段间隙(gap)无法从已有的公共数据库或自身数据库获得，可考虑采用RT-PCR的方法。在序列5’端设计引物，3’端引物采用Oligo-dT，在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最后拼接便获得全长。
通过组合使用上述3种方法，可获得人肝脏相关蛋白的全长编码序列。
序列表<110>上海人类基因组研究中心<120>人类肝脏中表达的表达序列标签I组<130>NP-10041<160>58<210>1<211>563<212>DNA<213>Homo sapiens<400>11 taacaagggg gcagtcattt aatgcagaaa tccagttaac ataaagagac agaggttgaa61 caacaggtct tcaaacaata ctgcatagtg tcacgagtta gctcttaagc acgtttgatc121 tgtttatctt atgatttcga ttccagtaca aagacagaga agagacttaa aaaaaaaaaa181 acaataaggc aacagaagaa gaatcttcct gtgacaagga acgtccacaa ataatgcaca241 aatatttggt gctgacacaa taagacacca tcaggacaag ttctgaagat gctgcattaa301 aatttaacaa gcatgataca atgacaagaa aattatactc gctttgttac gaatcctttt361 gcaagtagaa atgactctcg ggtaattgtt acatacaaac tttctgaaat aaatggaaat421 catacaaatt tttccattgc agtttctctt tctctacacc tagaaatgtc catggcactg481 ctccctattc tcacagtcac gcataatagc cttaaagcct tgaatccaca gtggcttgtc541 cttagaaggt ggaggttgct gat<210>2<211>659<212>DNA<213>Homo sapiens<400>21 acattttaga gttgtttact tttattaata aaaagttaga tcacttatat atatatacat61 tgaaaaaaac tattattgtc cctttcctgg tgaaaaggag tatacacaac agacctttgt121 gatattctgc aacaatgacc catattgctg tggaattctc actttttagt agaaccttta181 aaaactcaag tgagctgtac aacgccccga ttgctttaac tccagtgaac cacggaggta241 taaagacgct agagttcttt gtaattcttc agtgccaaaa ataataaagt tggaaacttg301 gattcctgtt tgactctact gtattaagtg atcagaataa aattactgta aggctaatct361 tcaaccactt catggttttc attcttgctt tctatagtga acatctggtt gtttccactt421 ggctctaaac ccctttcctt ttgggattca gcccactact catccatgtg attctgacag481 gaggttagca atgacagtgc agatagatag cccaggcctg gccaaagatc acaggcccca541 tcccctcttc cacagggact gggtccatgc aagggcaggt gattctgggg aagaccagag
601 tccttttata ganttgattg tggacctgaa aaggaagtnt cccctccctt tgancatgg<210>3<211>604<212>DNA<213>Homo sapiens<400>31 tnataaacat atngaaaact ttantagaaa tatanagaaa aanaaataac ttgcctgnga61 ggtttcctgt cccttcagga tggatganca acagatgggg cacctaggtc cagtggggct121 gctcccgatc tactgtttct tcctccagga gcaaggcatc tagctccttc cagccgctnc181 tgagcagggg gcccacatca gggctggggg ccacattggg aggaacagtc ctgccccctg241 cagcccggac cccacggacg gtcttcgtga tcgaggaagg ctgctgtccc gaaattccac301 agcccgccgg agcttccttg agctggtgaa aatcagagtc ccattgcgct cccggggttc361 ctcaaagatg gtctcaaagg tcctcctggt tgtggggtga ttggtaattc ttggttggtg421 taaatttctt ccaagctgaa ctccttcttg gttcaacctg attgggtcga ggcagtccca481 ttggtgtaan gtttaagctg aggacgggct ggtgttctgc ggnattctga atcgngaacc541 tttcccatgg tcttggtctc aaggttngaa agcacctggg tcncttgaag gctcttggct601 cctt<210>4<211>629<212>DNA<213>Homo sapiens<400>41 aacaaattgt attattacac attcctagtt gctgcttgta atagaacaga tttcaatctc61 agaaatgttt atctgagaga catagatcag gtttacaagg acaagaggtc aaagtcatgc121 cacagacgat ccacaccacc tatgtatgca ctctctctct tgcacacaca cacgcacacg181 cgcacacaca cgctgagtct catgtgctta cctcgtctga ctgccgctgg tgttatatgt241 agcttccatg cctggaagag gcacatatgg taacatggtg tggctaaagt aaagcaaaag301 tacaaaagca ataggtaagg gcagtgggat aacaggaaag gttttgatgt tattgtgggt361 attagcacat gatagcccca agaagacaag aaatgtcagt tactcagaaa gaggaaagct421 ttccaaccag gagagttata tttatgtgcc ctcactggaa tggaggcaga ctgacaggaa481 gattattttc caacttgaat ggaaagaagg cagagacaga aagaaagaac agtggctggg541 caagtggctg atgccnataa tcccagcact tgggaggctg aagttggcaa tcgcttcagc601 ctggagttca gacccagctt ggcacatgg<210>5<211>716<212>DNA<213>Homo sapiens<400>5
1 agatggagtc tcgctctgtt gcccaggctg gaatgcagtg gtgcgatctc ggctcactgc61 aacctcctct tcctgggttc aggcaattct cctgcttcag cctcccaagt agctgggact121 acaggcgcat nanccacgcc gggctaattt ttgtattttt agtagagact gggtttcacc181 gtgttagcca ggatggtctc aatctcctga cctcgtgatc cgcccgcctc agcctcccaa241 agtgctgggg ttacaggcgt gacacncgtg cccggctgag aacattttac atatatgcga301 aatagctacc cagtcctgat tcgactagag cactgagtga cacacattaa atgttaagtt361 tgaaaagact gtaaaacatt cacatatgac agctcttagt gaagtaagag cctctcatat421 tncctaattg tttgaaacat tccaggagag ctaggttccc gtattctcct ctagaagtgt481 gtatagggct ccctcccttg tttggngata ttnttgatat ttcctccgaa tgatgtttac541 ttgactctgg ggcaagctga gcttttccac agatttagtt acttcttcac taactcactc601 ntcagcaagg ggaaactccg atgaatnagt tcngtattgg naactggtag gttgttanca661 attatttcaa gttccgatgt atccgaatgg gnaagtgagg nccgtattgg aattct<210>6<211>392<212>DNA<213>Homo sapiens<400>61 tttgcaggtg actacgcatc atggtaagtt ttatacccta gtcaaaatga aaaatagcaa61 aaaccgcact gtaaaaaaaa aaaaaaaatt ggtttccttt agtagatagt atttcaaccc121 atcatattct tattataaat aaacagaaca atcttaagtt gacagatatc aaagagacct181 tgatctgatt ttggtgccaa gtgaggtctt aattcctcat tcctgcctta agtgcttggt241 gtcatacagt gggtggtaca aagctgtttt atgcctaata ttgctaaatg ggacccttct301 aattgatttg aaagacatta taacatattg tccagacttt aagacttaaa gagtaggaaa361 tactactttt agtggctaaa tttattcatt tt<210>7<211>488<212>DNA<213>Homo sapiens<400>71 gatcccagca gtgtccagaa gaagaccaag gacagaacag agactaggtt tggtgagatg61 ggacagattt tgggaaagat catgatgagc catcaaccgc agccccagga agagcggacg121 ccccagcggc gcctcagggt accccctcca ggaggtggtg gatgatgaag tgttgggacc181 atcaggtgag gggactggag aaagaagagg tggcatagga ttgactaaga tgaaggaagg241 gggccaggcg tggtggctca cccctgtaac cccaacactt tgggaggctg aggcgggcag301 atcacctgag gtcaggaatt caagaccagc ctggccaaca tggtgaaacc ccatctctac361 taaaagtaca aaaattagcc aggcggtagt ggtgtgtgcc tataatccca gctacttggg421 aggctaagac aggagaatca cttgagcctg ggaggaagag gttgcagtga gccgagatcg481 tgctactg<210>8
<211>569<212>DNA<213>Homo sapiens<400>81 gggtgaaatg agttactaaa tgtagcattt atttataagg aatgcattgt gaatagtttc61 tcagttttca ttatggaaag atgatgattt cagcccacat tcagtgtatg tttctaaata121 acacaatcga caggactgtc tgttcagtac aatggaggac agctttttca gggcaaatgg181 gatttcttga taatgctaaa tctgtcttgt cagctgaatt tcttgggctt tatgtggcag241 tgtggtaaaa atatatgatc agatttcact gttaagaaaa ttctttcagc aatacatgta301 gagtcaagtt tcttgcatgg ataactgaac atgtgggtta tgagatttta aaaaatgtct361 cgtgacaaac tttacggaaa tgcaacaatc tggacatcta gttttgtctg agagtggcgt421 ggatatgaag aactgtgctg ttggtgctga tgccacacta agttttggca gtcacactct481 tggttcttca tatttgagga gatgggatgg tgaggaggcc tgttggcttt attttattaa541 tgccaccatc tagaatacag attcttgga<210>9<211>581<212>DNA<213>Homo sapiens<400>91 ggtaaagact tttgtatagt tttcctgatg ccctaatttg ttccatttaa tttctataaa61 tattcaaagg acatggaata ggatctgtta aatcaacttt attaatccca ttttaaatat121 ctttcatatg cttatttttc tgcttgatca gcaaataatg aactaatcac tttcaaactg181 ctagtttcta tacattcaat tatctgagat ttctattggc ttttacattg tttgatatta241 tacgttttca aataaatatt catataaatg tctataggtc ttatagttct gttgtaggct301 acacatgaac agcaaccaaa aagaggaaaa atcttctttg tccccatgct ttttccttga361 attctgcact tttcatatta acattagccc ttgctttgtg atttgccttt gctttatttt421 accctgtctt aatcttaacc ttcccaaata tttccataca gggtgcacct ctcctagaca481 acacacagcg gagagtgtgc ttcccatgta ttcaatcttc gtctcttgaa aggagaggtg541 ccctcattac acttcactgt gacacaggat acccttggtt t<210>10<211>442<212>DNA<213>Homo sapiens<400>101 ttgagtatga atagggttta ttaggtcttt tataaaaata ttcactaact tttctctagt61 ggtctcatct tctattctgt aaatggctct tagaaggaaa tgttttccag gtacagatac121 ctaaatgggt atctatctca caatggcctg gagttctgtt ccctcctcat tccttaatga181 gtgcttgtcc tgctgtgccc ttgcattgtt taatttgttg ctccttttta cgaaactcag241 aaattcttga tccataaagt agggcttttc tgacgatcca tcatttgttt ccagatcaac
301 agcaaaacac aggaaaagtg catccagcac agtttcaaac acagataaaa aactatgggc361 tactaagtag gcaaaaaaag ctaccaataa cagagggact gcccgcacct ggaatgcccg421 attgtagtta aaagccatga gt<210>11<211>559<212>DNA<213>Homo sapiens<400>111 ttttcttaat tatttctttt tattttaata aagcaaaact acaggtaaat gttttgccag61 atgacttttg tgtacttatg ttctaacata gcacatgttt ggcaccaaaa gatcttagaa121 actactatga aaaaccataa gattttaagt agttttctga cagaaatgaa catttctgtt181 gcatccctac ttgtcactgg ctttcttaaa cacagaaata ttcatttcca tagtctttat241 cgctttttgg aggatctcta agattggtcc catagccttg acaatttgtt ccaacaaatc301 tgctgctgac tttgtgggat ttttggaatc ctcagttttt agtacatcct gcagtttttt361 caaggtgtct atcagtggtt ttggaagaac ctggaggact tctggttctc tcagatgtgg421 tgacatctga ttgctctttg gtgtcttctg tatagaagtc acttaattga agattcatga481 tggggaattt catcaagtgt gaaagagaag attccagact cccaaggatg ttactgctgt541 ttagcacaga gatgatgac<210>12<211>432<212>DNA<213>Homo sapiens<400>121 ttttatgtta agtcactttt gagatattta ttattagaaa ccatccaaca tgaaatatga61 attattaaaa tgtttttctc catgtttcaa accagaaaca gtaattcaga atgcaactag121 catcctaatt tttaaaatca aaataagatg accagattaa taaaacttag cattcacagt181 attcaatcta gaaaaaatat aattaataga atgttaaagc caaaagaaca tttgaaagac241 agaaagagag aaggggagga gagaaagaga gagggaaaag agaaagcaag aaagaggagg301 aggaggagga ttgagagatg gagagtttga gattgagact tcttatgaaa tatattttaa361 cggcaattac tcattaacaa ctgtgcattg agaatatttg aacatgttgt tnaaagctag421 gggaaaaatg tc<210>13<211>501<212>DNA<213>Homo sapiens<400>131 tttgaaacat gacacagcct tttattattt cagatcgtga gaccaggtca gaagggtcgt61 tacgctcatc atgatactac tgctttgaat accacacaga ccagcgacat ttcccattca
121 tttccccaac cttgtgggaa aaattttcag gagtacagca tcaatgaaga acacagtgtc181 atgaaacaca aatgctgtca cagatgacag tcacgttacc atgctgaatt gtccagagaa241 gctaaccagt tactactcag ggctgatgta caatagatgc aacgccaaaa tgagatgaaa301 gagaattcag aataaaattc cgtcccttgg agcatattcc attggctttc tctgctcctg361 cttccgctgg tatctggcct tctggtagaa aatgatgatg gttacagtga ctatatttag421 aagaatgacg agcagtatga gccagaacga gtatccgtaa ctgtgggtcg ttcctttact481 ggtggttgcc gggtanagca t<210>14<211>607<212>DNA<213>Homo sapiens<400>141 ttcagattta tttttatatt tattaagcag taagcaaaca gagttggggg agatgaccta61 aaatggtttt cttttggaat aatatttttt aaaaaatcaa ataactgtta tcactttaag121 ttgcatgagg ctgaagctgc acctggagag gaatttaatt ttcatgacat gcaaaactga181 ttaggcagtg aactctttcc tcccgcttcc cctgaacaca tttataaact tcaacggggg241 tgaattccat ctaatactct gaaaaccatt tctcatctca cagtttaccc aaatcatgtg301 catgactgca agaggaaata aacaagaaat ctttaagcat agataagcca caatggatag361 ttaatcttgt acagacaatc cagcattaag gctaatttga tcttttaaac attttatggg421 aaacagctgt tgagttttct ctagaaaagc ccacaaggtt ccataacccc ataggcatac481 agcctcgaca ggcagggctc catcaggctc ctcttgaact gggcactcct tcacggaagg541 caggaatgca cgtgtcttca cttcctgctg ctctgcacgc acgtcacgat atctggcaga601 aggtggt<210>15<211>502<212>DNA<213>Homo sapiens<400>151 caattcttaa tcatttattt tcaacacgtg tactataaag aacctaagaa atcagacaat61 tttaataacc cataaacatg ttgaatccat ttgcagattt aaattttgta aaaatagctg121 tcactgcctc catatatcaa gtgtactatg tttataatca catttatgct accgatactc181 ctcagaaaaa aacagattct gcttggttct agcttcagta tcatgaactc caataatgcc241 ttgaggacct ccaaaggaaa aaaacagatt ctactaggtt ctagctgaaa tattatcaac301 tccaaattga agctgaagta ttatcaactc caaataatgc ttcgaggacc tccaaaaaaa361 agagattctg cttggttgta gctgaagtat tatcaactcc aaataatgct tggaggacag421 caatgtagat gattttgtaa tcacctttca tcggagtgac cttatataaa aaataagtca481 ataatttaga ggttctaagt ct<210>16<211>576
<212>DNA<213>Homo sapiens<400>161 tattcacccc tcccttgaag gtttaataga caacaggaat ctgggctctg aaaagtcaca61 aacacctttc ccaagatgat gagcaactgc aacaactggc caaatgtgtt agcagcagaa121 aagctgggca ttacctgtgt gagaggcgct cagcatgggc agtgaatggc cgtgagcagc181 gcaccagcta catagcctcc acaccggaag ggtgctgctg ctctgcaggt ccgtttcagg241 cgggatccag ccatccacag gaccaagggg ctggagtgtt gggaacagat gattctgtgt301 tccacagata atggtacaaa gtgactgttc atagataagg tgtaagcaca gcatttgaag361 acagctcctc cagatggtct gtctgttagt gaattcttaa gcacttagag ccttgaagat421 tgagaaattc taaagaaatt ttttaaaagg aggagggtgg tagatgtcaa agacacaaaa481 gctctttgta ggtgctgact ggctcacttt gtccccaggt tcatcttaga gctcaagcag541 attcaagaca tgaggcagag atgctagact tgcgtc<210>17<211>450<212>DNA<213>Homo sapiens<400>171 ggctagggcc agcctgctgc aaaccctaga gttcacaaca aaaccaccag ctctgcctac61 agagccagtt ctttccttcc acacccacat gagctcacat accttttcca tgggatcctc121 ttacagctgc tttcattcca gctaccagat ggggacttgt atgtggccga gcataaccgg181 ctgtgaacaa gcagcactct gtctggggag cccagttcag catctgaacc cccaggctaa241 agggagagct ctggagccaa cacagcactc agtggtcagg aagggggacg gtgcggcggc301 ggcactcgct gcttctcacc tggcacgggc ctgcaatctg ggtcactgtc ctgagtgcgt361 ggatatctca taaaccaaga gccatgcctg tgcctcgggg tgtggttgtg tacacgcggc421 cacaagtgca cacggctaat ccagttttgt<210>18<211>562<212>DNA<213>Homo sapiens<400>181 agagatttac aaagattttt atattaattt aataaaacaa attcttaaat agaacaaaat61 aaacacctaa tgagccactt atatatagaa tgcttgtgcc cgttggctct cgttcttacc121 ttgggccctc ttacctgcca aagcctacaa ggcccttaat tctaaagcca cccacccttc181 ccacaaccat aaaaacgtac agtccagtga ccttggaatg caggatctgc cagaggcctc241 cactacgtgg gcattaacac cctggctggg gtggggccct gtactgtagc cttggccttt301 cattcagaca gaaaggctct accttctttc ctccccaaga agagacactc cctcatgact361 gtcactcact ggcacaattc ctgcctctca gcaggcatca gctgacaact cccggccacc421 cctgtggccc tcttgtggcc ctctctagaa gaacatgaag tcccagctct gtggcaacca
481 gagccatcct ttcttccacc tcccgctcag agagctctgc gtgaatgggc agcccggctg541 gctgtgaggc gccagcacac tg<210>19<211>492<212>DNA<213>Homo sapiens<400>191 ttttatatta agcaataaac tattcggttt attttaacat ttgagaattt cttttgtata61 taaatgacat taaaagattc tcagaagaag aaaacctaag ctgaatggat taaaggctgt121 ctgctgaggt gctctccccg ttggcttcag aaatggattt tggttaggta taataagttg181 tggagtcctg cagaggttct ttacacattt ctcttctttt gagcagagag agcaagattc241 tcctcttacg taaggaggca tatttgcaaa atttcctgca ggtccgtagt tgcatacaaa301 tattgcagtt gaagctcccc caaggttagg acacattgca actgcacaac cgacataaaa361 tgaattggcc caaactaact gtgtataatg gccacagact ctggagcatg atagactatc421 aaaatcataa aattgggttt cattatacca agccgtaatg gcatgtcttg gtgtgaatga481 ctttattcca cc<210>20<211>528<212>DNA<213>Homo sapiens<400>201 tttttttttt ttaaaatata aaggaatttt atttatgaat gtatagatgt acagcaaata61 ggaaagataa tacattaaaa gaacatatac tataaccagg tagagtttta tctcaggaat121 ttaaggatgt tttaatatta caaaaaccct tattataatc atattaggca atttaagtga181 taaaaatttg attgtatcaa aagacaccaa ttccttttat caagctttta gcttccaaca241 aacaagaata gaagatgtta tttctgttat cctaacaaag aacatttgtt taaaaccaac301 aattaacttc atatgtaatg aaaaaatatc aaaaaaactt attacaattt tatgaaacac361 aaaaacaaaa tagaaatacc tatgatttta tatttagaaa gagcaaacag gccatctaaa421 actgttagaa caaagaaaat caccaaaaga tacatatttt aaaagttaat agctttctaa481 taatagagat tcccatgctg acagcaacat agatataaaa cagccaag<210>21<211>443<212>DNA<213>Homo sapiens<400>211 tttttaagta agcacagcaa taaagtttta atggaatagc tttggcccag gcagaggcag61 ttcccagatg tctcaggacg cgtgtgaacc ccaagaagtt gggggcgtta tctgggccct121 tgggatccat tcccctgggt ataaagtgtt ggtacgtggc tccaggcctg gcccactcag
181 cctcccccag gaaggcttct caagtggaga aggttcttgg cctcagaact cacggcgctg241 ctgctcacgc tgacccccca ctagatgcag atcggtgtag aaaggcctgc cagggcaggg301 atgagggccc caagctggaa gcagcccaga aatcctttta ccagaggaca actccctaat361 ccgctgggca ggggcggggt gtcacacgca ggattccgcg acgactgtca ctgcctcctg421 ggcgcccctc tgtgcgggag cgg<210>22<211>474<212>DNA<213>Homo sapiens<400>221 tttgttattc agaatatttt attttaaact acaaattagc acagctaatt tgaatattta61 aatttccgta agcactgtaa attgaataca ttttttctta atgatatagt ttttttccta121 aaaatcctag gcatattcag acttaattca aatgggttgt ctaaaaccag acaatacaga181 tgtggagcca tttgtggtag atggacgtgt atatcttgca gaagtctcca atggccttaa241 aaagcattat tattggacat caacttatgg agaatcttat gaccactcat ctaatgcagg301 ttttgctctt gttccagtag gtcaacagcc atgaaaaagt agctgtaaaa taattttcat361 acacttgtct tattgtttca cagagattaa tattctatag cctattttct tttataaatt421 aaaacaagtt acttttttca aatgtgtaaa agataatgac tgtattaaac tttg<210>23<211>266<212>DNA<213>Homo sapiens<400>231 tttttttttt tttttttttt tataaaccac cacagagctt acaaaataaa caaaagaaaa61 attgaaatta taagttcaaa gagtaggttt taatttaata agctattggg tatttagggg121 aaaaccaatt aaacttggaa ttgcattaca aatttgaaat tgtaatgaag caagtagctt181 ttatgaaatt ggaacataaa tataatatca acaaaaacat aagaaattcc tattccaatt241 aagtctcaaa atatttgttt tacagg<210>24<211>545<212>DNA<213>Homo sapiens<400>241 ttttttttaa gtattgctca cttttactgt tctctcttag ggaagggcat tctcaaaacg61 tttccgcaca agcagaccat cccttttatt ttccccgtct gtctcctttc cttctgcttt121 caaaatgtct caagagtatt tacaagagtt gagcaacaca ggcatcttta tctggggtct181 ttatccacag agcagaggac aggaagtcat cactacagag acgaagcgat gtatggtttg241 acccagtgga ggactttgtt aaggtggagg tgtgagtgtg gagtgtaaac gtgggacatc
301 cagggcagtg gagggtaacc actggagagg aagtctgggg accagtttgg gagcagccaa361 atataaaata agcatttctg ttctaaatcc aaatgaacat tctacgctgc tgtcatctcc421 agtatacctc agagatccta aagtttcctg aagttgtttg gaagtcacgg ccgatgtaaa481 gtgaaagcgc acacatatct gtaagctctg agctcaggat ttcacgtgag gtcctttgtt541 cggga<210>25<211>724<212>DNA<213>Homo sapiens<400>251 aaaccttaac ccagagttat ttttattttc cagaacgtgt taggaactag tacttaaata61 atctcaagtc cctgaggggc cagagatccc accatgcaaa atagcaaaca gacccaagac121 ttggggagag gcggtgagtg catcagaaat ggatgggtac atctgattcc caccacgcgg181 ggctcagctt agttagcagg agaccttcag actgagaaaa aatgcaagtc tttttttggc241 ctctaatatc tgggaaggat ggagggagct caggagacac agaaaagatg gcgtatgaat301 cctgtccggc ctgaacgagg ctggagttgt gcctctggat agcttcaagc actgatcaga361 ttgtcagccc ccgctgcttg aacagatgct ttagagcctc ttccagttgc cggtttgttc421 cctgaagccc cttcaccacc tgcgctgccc actcgaagta ttcctggact cgatgttctg481 accatccctc tggggtgcag cgattcaggt ccctcagatt gtacagcttg tctgccagct541 tcaccagttt ggccccgggg ctactgtggn gcgcttggct cacctgcagc ctctntctct601 ccagcttggg cagagtcttg tcatctggta cctnctncac caggcgccgc acttgtgccc661 caaagtgtag cttcaccctc atccaggtgg tgtctgtgtc ctccaccgtg tcatggagca721 gggc<210>26<211>544<212>DNA<213>Homo sapiens<400>261 aaagctgact ttatttttat ttgaattggc caagtaaaat gttccataca aggcaaacag61 ttcaacatga aatatttaaa ttaaaaataa attacttcca tcctatataa atctctagct121 gccacataca aatgaaagat accagttata aatgaaataa gttaaaataa atggttgtaa181 ggagctaggt gtggtggcat gtgcctgtca tcccagcttt caggagactg aggtgggagg241 atggatctct tgaacccagg agtttgagac cagtctgggc aacacagcaa aatcctgtct301 taaaaacaaa cgtaattgta ggatcctctg ttacgaccag cataggttac tggtttgctc361 cctaactttg ttctggactc caacatctct attgaatgag acttttgaac agtcctctgg421 gatagcccct tcttaaaaga gcacttgatg cccaggtctc caaatcactt tcagatatga481 ggatctggtg ttcagagtct aaaacaatgg ttgaagagca gagatagcaa ggtggaatcc541 caga<210>27
<211>639<212>DNA<213>Homo sapiens<400>271 tttttgcctg tgttatcgat ttattgcagc tccaatatga gtccactcct actcaaaccc61 tgattctcag gggcctgggg aaggccaccc cactaggggc cctgtcccca ccagagcctg121 gacatgggac tctctcccaa gcaggggtgt tgtccttcac aggggcttcc acgtctctcc181 cgggcctgtc cgcactctgg gcagggctgg cacctggcta ttccctcaat ctgggccctg241 gggccatggc aatgtggaat ggtccaggtg ttcatgtcag tggggcttgg ggacatggcc301 atccacgtag aagttcctgg tgatcttggg cagggtgaat tccgctcctt ccagctccgg361 tgtcagcaca atctggcagc ccagccgcga gttctcctgg aggagggggg ccatgtctag421 catgtcgtct tccctctcct cgggaggagg caggagatcc aggtggtctt cactcacata481 cacatggcag gtggagcagg ccagggaggc ttcacaggcc ccttccaggt ccaccccgtg541 gcgctgggcc aggtgaagaa cattgtcccc gactctgcca ctcacttgga tccgctggcc601 tgagcggtct acgaacacca cgttcaccac gtncccggg<210>28<211>489<212>DNA<213>Homo sapiens<400>281 tccctgtcaa tgtgttctcc gtcactcctt acacacccag caccgctgac atccaggtgt61 ccgatgatga caaggcgggg gccaccttgc tcttctcagg catctttctg ggactggtgg121 ggatcacatt cactgtcatg ggctggatca aataccaagg tgtctcccag tttgaatgga181 cccagctcct tgggcccgtc ctgctgtcag ttggggtgac attcatcctg attgctgtgt241 gcaagttcaa aatgctctcc tgccagttgt gcaaagaaag tgaggaaagg gtcccggact301 cggaacagac accaggagga ccatcatttg ttttcactgg catcaaccaa cccatcacct361 tccatggggc cactgtggtg cagtacatcc ctcctcctta tggttctcca gagcctatgg421 ggataaatac cagctacctg cagtctgtgg tgagcccctg cggcctcata acctctggag481 ggcagcagc<210>29<211>537<212>DNA<213>Homo sapiens<400>291 ttttttgtgg tggtcactgc tcagtgtaat gtgcagaatg atttgttttt tgtttttttt61 tttttggtcc ttcattgcat cctgccatac ccatgagcaa acagtttggc attaattata121 tatcactgcc accctctgaa ctttgaaaac tgccatcttc agacttggta taatggaaga181 ggctttctct ctccaataaa ccttttgctt cagggtatac tcttcggttt ttttccagat241 gtattatgta tgaactttgt actatgtata gccagagttt tatttatttt ttaaaaaaga
301 aactttttct tgataaagga ataatggtgg tctagctagt tcttgtaaaa gtgatgcctc361 ttgaaaaaaa acagtcctat tcactagctt ttagtaaaag aatcagatct tttctttctt421 gttaccttgg agtcttaaaa actgattgct aaggtgaaac aattcaatgc ataagtatgg481 agctaagtgc cttttggagg atttcttgga agagcatcta tggagatact taaggag<210>30<211>469<212>DNA<213>Homo sapiens<400>301 gctttcggtg aattaaaatg tatttggtgc cagtggtgct gaacatagaa taaaaaacag61 aaaaagggcc cagacaggga catccaggct ggaggttgga gtggagcaca gtcagatgta121 ttcacttttc tagcatgggt ttcaaagtgc gtaaggggag aaaacgcatc atggattgtg181 ctgtgcatcc tggacaccgt gcgaggtgct catagctgtg atcttgtctc ccacaactct241 cttgggtgac agacgtccac tgtccacatt ttatagacaa ggagaaaggg aagtcaaatg301 tctcgtccaa gtctacacag ctaaaaaggg gcagaactag ggtgacgctc aggcctcatt361 tagagatcgg gggttggcga gaagtggggt gggcttctgg aggggctggg agagccccac421 aaggctgcag agggtggtga gcccggagtg ggcctggcct ggtgtgggc<210>31<211>496<212>DNA<213>Homo sapiens<400>311 tttaaggggc gagagatttt aattaacaca aatggctgtg cctagtcttt aaatctgtcc61 aaagcaaatg gagtgaaagc ctgctaattc ttcagtgctt catctggcaa tgacctcaga121 aaaaagagtc tccctcctgt attatcaagc tgacttcctt tagttccttg ttgttctgtg181 tagatctttg ttcccagaat tctcacatgg tgttcatttc tgccagtagt cactctgtcc241 atagttgtaa tgcccatagt acatccaacg ttcaggtttt tctcagaaga gctcaaggaa301 atgcttggga cccaggaaga tttaaaaatg cttgcattgg ctcaaaattc tgctgcaaat361 acagtgataa tttcaggtca ttggccttct ggtagttctt caaggcttca attctcagct421 caatcacttt gccatgaaaa tcattctgaa ggatgttatt ttgttcctga tacttgaccc481 attccatttt cctctg<210>32<211>577<212>DNA<213>Homo sapiens<400>321 aattttattc catttttaat attttggtct agcaacttgt gatacataga tgacaatttt61 gtgagttttt caaatgtgtg tacagatttt tgtaaatatg actcttttgt aattaactca
121 tgtacagcct catcctgtat agtttaatga tgaatgtgca ggggacctgt ctcaggctcc181 tatatggttc ctgggcctta tagccaggtt tgtgtggcgc tcccgacttt tgtgactgac241 tggtgtcttc ccatttggac tgtggcctgg ccagagcccc ttgcatatcc ccactgtcag301 gggcagccag gactgttccc atcctcctgg aatgggggaa ccttccttat cccctgccca361 catcccctct ccaataaagc acctgtgccc tcagcaatgg cctgccatgt gctgntgctg421 ggatgtttgt attaaggtaa gtgtgggtat atggttttga gaccgggggg tgttgaacat481 tttggggaat ttattatggg gcatcttaag ggaagacatt ccttgcttct ctgaaggata541 cagcctccag ggggagactg ccattgtggg taagtta<210>33<211>528<212>DNA<213>Homo sapiens<400>331 aattgagaca gggtctcact atgttgctca ggttggtctg agtaatgata ttatcttgca61 tttatgtggt gctttacttt ttgttacaca ttcacagtaa ttaacctcat ttgattttca121 acatctctac aacagaggtt tggcaaatat tttttgagtg gctgctaagt ggcaatcatt181 ccacaaggta tttttacatt tgttacttca cttaatcctt tcaacaatcc tgagaggtaa241 gcatttgctt tacttaacag aggaggagac ggagactcaa ggaacttaaa tcttaaaaaa301 aaaaaaaacc caaaacaccc aaaaacaatt atttatgttt tacagataag agactataaa361 accaattaac aggtactttc accacatcag cttgtaagat gaaatggttt atcatcctaa421 tgttagtgaa tgacagaggt gtgcagtctt acaaatgaca cactttgtaa gtcactgagc481 aatgtttttc tgccataagg gattttgagt ccgagaaatc aggtcctc<210>34<211>577<212>DNA<213>Homo sapiens<400>341 tttatgatat catagtcttt tgactttatt ttctgagatg gaaaagtata ggcataggtg61 tttttaatag tcttcttgat gatatccttt agaataatct atcaaatggc ttctttcatg121 tttcctgatt atcagcattc atcagtgtta ctgtcagcct tgattaagtg gttgaaaatt181 tcagagaaga ataagcaact tctgtgaacc tttccccaat ccctgagaat cataatcatt241 acagaaatat tttatccttt catcaaatat ctctggaaag tagccgtgtt cgttcattgt301 taatagggca aggtgcagtg tcatagaggg ttaatccatg taaattggct tccacccgta361 aagaaatttt ctgaaaatca cgaatgtaag tcaggaaaaa accaaagaag gaaaatgaca421 tagaccattc tgctgcagta gtgatcatgt gaagcacata acctttgtcc tcggggttcc481 aatggagttt ctgttctaaa tcagtcccaa aattgccact gtgcaaaact gatgagcaag541 tcagcatcca caccagataa ccaacaacag tctgatc<210>35<211>465
<212>DNA<213>Homo sapiens<400>351 tttattttta ttctttttta tttaaaagat tcccttttta atctgttcca aagtgttgga61 agctgaacta tacaagttcc tgccacccat ccttccagta cagttttctg aggacatact121 ttttaaatgg aaaaataaca ggaggaccct ctaactgaaa ccaggtaggc ccagagtgag181 ctcctcaggc ccctgtggca gcctggccaa agctggcctt tatggagcca gcgcgtggag241 aagtctgggg aaccggggcc cttccttctg gtgaggaaag ggctgagcaa actcatgttc301 tctttagtga aatggtccaa attcagatcc aaaagcctcc attatggcat tccctggcac361 tcttcttata ctttatacat tgaaggcagc tccctctttt atggccagta taagcaggca421 aagaaacaaa ggaatataga tacatatatg tgtatatata tatat<210>36<211>698<212>DNA<213>Homo sapiens<400>361 ttttgtcaat tgagcagatt taatttccct gagattatat tccataagtg atacaaatac61 taccccttag accaggggtt ttcaaagttt gctgtgcaaa aaatcagcta ggtatcttat121 taaaatggtc cccaacatgt ctgagctgga gcttcagtgt tcacaggtct caggtgatgc181 tgatgctact ggtctctggg tcacacttta aagaggaaga cattagataa agtaaaaggg241 agtcatgang aaaagggtgc acctaaaaaa catcacataa caaaatacta aagtccccga301 gtgggagaat acaaacatgt ctctctactc tatatctaag aatcagcnnt ntggttntct361 atattnncac tgtgtctgat tataaagact atnttaagnt tatggggctt ttttaacttc421 aaanntnaaa gtgctcccga ttttttaaat aaaacacgac tatagggctt tttagaactg481 gggctgtctt ggagagtgaa ggacactttt gggagatcaa ctcttttgaa atttatttca541 ctgctaaccc tcgataatat tgcttttttg aggctaactc cagagaaatt aattggctct601 aaggcaaggg catttattat attaataaaa gnnnnngggc tcctcgaagc acttacatta661 cataaaaagt tcacacattg gaaactantc ccaaaagc<210>37<211>688<212>DNA<213>Homo sapiens<400>371 tttcttggcc tgcagcttca tttgcgagag cgccgaggca ggacacagag cacagctgtg61 ctggaagtgt ggggagaacc cggacagctc agtcctgcca gcagccgcaa agagccgagg121 ctgccaggcc catttatgtc cctcatgtct ctagattttc tcgtcaccca gcctcaaaaa181 tatatgtgtc tgcaaccctc agtctctctg ccgtttcttg attcctttcc aaacctcagc241 cggggccctg gcctcagccc cccgtccctg cgtctgctcc tcttcacagc agctgctgct301 ggctcctccg cgctcggcgc caggccctgg gtcctcagac gcatcactgt catcagtgtc
361 cgagggcagc cgggtgctgg ccacaggcct gttggggttt ccaaactctc tccctgaatc421 cacatcgaag ggatcgatct cctcctcctg ggggctctcc ttggcattgg ccaagaactc481 ctgctccgcc tgcagcacct cctccgggct gccgcaggcg cgtactttgt ccaggagctg541 cggttcaggt ccaagaagcc cttgccccat ttaaacttcc gcagcaaaat gacagcaagg601 tctggaaagc ctacgatgca gaaggtgcag caaacgcttt cacgcaggaa agtctgtagg661 ggcggccata gttcacggng ttgcgggc<210>38<211>544<212>DNA<213>Homo sapiens<400>381 ttttatgtta cactgtgcat ttatttacaa agaggttgga gaaacacaca gaatctggcc61 cccctttgca ctggggagtc accatcgtca taataaatac agtaatttta aaaaagaaaa121 aaaatactag attggaacag ctgagggtca tcaggcttcc cccattccct ctcacaggga181 gtgggggtca tgagatagaa accatcttcc ttcccctctt ctacctccga atcctccata241 attgtccagg gatcgaagtg aaagagtcct tctttccagg tgttaccaac ccctacttga301 ccctcaaaac ccccaaatca gtgctccttc ccttcccagg gcactctgca gggaccacct361 gagtcagaca cagagtctct gaccagggtg ccaaggggat atcttaaggg tccagggaca421 tggagggggc actttgtgaa tgagaggggg ccatagctgg gcaaggaaag gaggaatgag481 agggctcatc aggaatgaga gggagagcgg tggaggggaa caaacagact aggcttctaa541 acta<210>39<211>542<212>DNA<213>Homo sapiens<400>391 taaaaatcat ttttctttaa tacaaaatca cataaagaaa ggcatgttgg ctaaatcaaa61 tattcactaa atatcagtga agtcaccact ggaatctcaa tagcacattt tcctgctttc121 ttttctccct tctgctaacc attgaagacc agggtcatcc gtgggagcag atgagtagga181 cacgcgtctg cacactggag gccctggggg ttgacatggg agcaggaagt ggaccccccc241 accctgcaca tcccttctgt ttttcttgat ttcagtctca ctggcccagg ccaaatcttc301 aagggtgtct agttctgcag ccagggagaa agtgatgcca agagaacctc gtctcctccc361 tcctcagtct gctttgaagg ggaaataaat acacaggcct agtgtgtctg tgtggcacag421 ggaggtggtt ttgccaggca tcttggaagg ttgtcttcta gaatcagagc catagcctta481 cttgtggcct tggatctagg tctgtttccc cgatcgaaaa aagaacagct gttttatgat541 tg<210>40<211>542<212>DNA
<213>Homo sapiens<400>401 ttttttttag agttgtaatt taaatatttt attaaaatac atattacaag aaagctaaga61 tttacaatga acagagttat gaagcttctc aactgtcttt aaacgtttaa caacacaaag121 ggactagctg tcctcaataa agtaacacag taacaaaaca tgtaacaaat tattttaagc181 acagtttaaa acaccctata gctaaaacac tcatactatg taaatccaca taaactctga241 aagaagaggt atttttccac caccactcca aggaacacgt aaattggcat agctatatgg301 tagtctcata tatgaagata aacaaatagt aatgcattat ttagtacata attattacac361 acaaaaggta aggcacagct caagcttatc taataaaaaa tatttaaaaa tgacaattca421 taattgcaaa caaaacactg aaagaaaaaa ttcggaagaa tttaataaaa ttttgaatat481 ttacaaattg caaatgtgag tacttgttat aaaactactt acacccatgt agaatgtgca541 tt<210>41<211>624<212>DNA<213>Homo sapiens<400>411 tttgacaccc aagaccatcc tttattgtag tattagttca tggtaactgc atgaaaaaac61 atttcaggag gaatttacaa tttccagctt aaagaacttg cccaccaaca taaccaattt121 atgaaagtca attcattaaa aggtatagaa cctcttgttg ggcatgatgg caagggacaa181 agctacaact tggcctgtgc ctttggaagc tgaggcagga ggaccatctg agcccaggag241 cctgagacca gcctgggcaa catagagaat ccgtctcaac aaaaaaaaaa ttttagccag301 gtgtgctgtg agctgtagtc ccagctacaa ggtgggagga ttgcttaggc ctgggtgatt361 gaggatgcaa tgagctgtga ttgtgccacc acactccagc ctgggcaata cagcaagact421 gtctcaaaaa aaaaaaaaaa aacccaaaaa aactcaaaaa tgtaatgaat gatacccaat481 gtgccttttc tagaaaaagt tgccaaatat atctcttgga tctgctgagc atgtcctctg541 atacataagg caagcatgtt tctacaccca gtgttgatgc cagttagttt tcagaatcca601 aactgatggc agctactggt cctt<210>42<211>500<212>DNA<213>Homo sapiens<400>421 cgtggtttcg tctatttatt aaaaaatatt tgagaacaaa acctctgcct ctttgagtct61 tgctctggca tccccagcat ctctgattct ccctggtgcc cccagctcag gaagaaggtg121 gtagtgggga gagagggtca ggggggcttg gcagggatgc aggcaccatg acttttgtga181 ccagttccta gagacgcatg ggtgtagcct caggaggaaa gcgagaggag ctttaccatg241 ggaacgaagg aaagggacaa cattgggagg caaacgttgg gagactagtc cagaaacttg301 cagttgagga tacaacaggg tcctagtgtt tcctccctgg gtcagcgtag aagtagagct
361 ttttcactgt cgaaggcggg attgaagtta gagattgccc tggcagaggt tggggagaga421 gggactagga ggtggtacat gaatgtcttc tctcattctg ctaggtccct ctcagtggaa481 gatagatagg ctggtgggtg<210>43<211>475<212>DNA<213>Homo sapiens<400>431 gttaaaatat cttttaattt taccggcaaa atacatcaat gactaatata ttcccgtatg61 gaatatattc ttattggaat atattcttat atagaatata tagactatgt atatatacat121 atgtgtatat acctatatac atatatatat cttggagata taaaactaat tgggaataat181 gtcatatata tagatagtaa gcaaacattt tttaaaacat tttaaagact ccaaactatt241 catttctatc cacctataaa aaactagcaa ttaaacctgt caaatcacag ccatcttgct301 ttacgcagat tcagaagcat gagatccttt tacctttgaa actctagttt caaagctgtt361 ctaaagtgac ttggttttaa tttatatact tgcagggata agaagtagct gtagggccat421 aaaaatgtaa agaatacagt atcgtgccca tacacaactg caaacactta ttttt<210>44<211>487<212>DNA<213>Homo sapiens<400>441 ttttttttgt tctcaacttg tttaatatgg aaaaaggtca tcaaaacggt aggcaggttg61 ctgtttttaa tacattcttg ggatgttggc cattcagaca gcattatttc aaatatagat121 gagttatcca actttttttg actcaaagat agtttgctta gatttttttt ttttgagatg181 gagtcttgct ctgtcaccag gctggagtgt agtggcgtga tctcagctca ccgcaacctc241 cgcctcccag gttcaagcaa ttctcctacc tcagcctccc aagtagctgg gactacaggc301 atgcaccacc acacccagct aatttttgta tttttagtag agacagggtt tcaccatgtt361 ggccaggatg gtctcgatct cttgacctcg tgatcagccc gccttggcct cccaaagtgc421 tgggattaca ggcgtgagcc actgcgcccg gactagattt taacaagacc taattccatg481 ggattat<210>45<211>493<212>DNA<213>Homo sapiens<400>451 aactgttaaa aatgttttta ttttttagta agcaaaattt tacgaaaagt cagattcacc61 cgtggagcca gaaccttctt tctcacggga agactgtggt accagtgcgt gttggtcggg121 tggttcatca ttagtaagct cccgtgggcc agcggcagcc tgaccaccgc caccctcctg
181 gaggggcttt tcccacggga atccttatgc cggaagacaa agtctctgca ggcaccgaag241 gagacagagg caatggggct cccaggggcc agttctcttt catcatctcg gtgctccccg301 atgtggtcac agccatcttt atacctgttg atgagcacaa agttgaaggt ctgtccagtc361 accccagaga ccgtgatccc ggatgcgctc tagactgggg atccagggct ttggaaccag421 cgtgaggctg anaatgtggt aggtcagccc agcgtcgcca tacgttgcct gcttcctggc481 acactgtgcc act<210>46<211>361<212>DNA<213>Homo sapiens<400>461 ttgattttga taggaaagct ttaattggtg actaagcttt gtaaaaagga tgtaaacagg61 ggaaagaatg aaaagaataa gatttatcta cataatgaag tttgactttt tacagtaaat121 aaaataccta tttattaata tttccaaata ttaattagat ttaataatac tatatatttg181 ttacaatgcc ttcatcagaa aatttaataa ttaagaagat acttgaagtt cattcaaatg241 gcacttgcag aaatgcaact taatttctag ttctatcata tctgtctcca aaatggacat301 atatgacagc aaaaacaaaa ttctctataa atctctccat tagttacatt actatgtaac361 t<210>47<211>595<212>DNA<213>Homo sapiens<400>471 agtttttaga aaaaaagaaa aaagtttaat agcgatatag aaaattgagc acctataaaa61 ctatatggtg ggctctgggt tacattgagc ttaagtagac caagtatcta tcccacctaa121 attatcagca actccaggcc atgacccagt gtcccattct ccttcacggt ttgctggtgt181 gtagcctcct ggatacaggc aatgtatata gatcgcagag aatgtccgtt ctggcctctt241 ccttggaagg gtctaaaaac agaccttaag gagctaagtg acttccccaa agcccacagc301 tccagtgcca ctgctgactt ttgggtgggg gtgggatcag ttaaagagga aagctttcag361 tactgtcagc acatacacct gccaattaag gggctggata gagttcatct ctaggagcag421 gacctccacc cccaccctcc accccagaag ccatgtcatg ctgtttcacc aggatcttgc481 ctgaggttag atgggagaga acagctacaa gcttgggtgg agtcactcat aaacacaccc541 tgaaagctgt ggagcangga gagtgcangc tntagagggc tctncatgat ccctt<210>48<211>503<212>DNA<213>Homo sapiens<400>48
1 aataattgct tgatttattg tacagaagtg tccacgggct cacaattcta aggacatcat61 catgacagca agtaggaaaa aaaattaaag taattctttc accaattacc agtttatctc121 ttctccaatt cctttggata cgcctcctgc aagaagccga tttccagttg tatgtgataa181 tgaaagggtt tttctttctt acgttaaata caagcgaagt gattaactgg aagatagcgt241 ctgattgcga ggtaatcagt gattcagatg gtgtgggaat ggcacctggg gatgggggag301 gcaggacgga gatggaggaa gctggtgcag cctagcctgc cttgtgccaa ggacacccaa361 gggcagaggg actgagctct gggggaggac agatttgaca taactggtcc agcctcacag421 tttacaggtc ctggagggtg aggaaagtgg gagctggctg cggatgcagg acaagaaccc481 agggcttccc ttcattttgc ctg<210>49<211>535<212>DNA<213>Homo sapiens<400>491 ttttatttat ttaatttttg gagacagggt cttgttctgt cacccagact ggagtgcact61 ggtatgatca tagctcactg taacctcaga cttctgaggt caagtgatcg tcctgcctca121 gccttctgag tagctaggac tccaggtgtg catcaccatt cccagctaat tttatttatt181 tatttatttg tttgtttgtt tgtttgtttt tgtagagaca gggtctcact atattgctca241 agctggtctt gaactcctgg gctcaaacaa tcctcccacc ttggcctcct aaagtcctgg301 gatcacaagc atgagccacc atgcccaacc ttttctagtt tttaaatttt catacatatc361 tattagatgt ttatagtgta aggttgaact ccattctttg cttctctgtt gctggtaaat421 ttttgggtat atttttacga attttgcaca ttcttttaca tattggtaaa ggaacagtct481 gtgatctgga tttattcaga tccttattca gaattaccaa ttgttttatt aatca<210>50<211>397<212>DNA<213>Homo sapiens<400>501 tttttttttt tttttgttca ctagtttttc ctttattttc ttgggtatac ttaaatagct61 actttaaagt tctattatta atttctatta agaaatgctg ggtctttttt tttttttttc121 cttgattatg ggtcaaattt tcctgcctct ttgtatggct agtaattttt taattgaatg181 gtaggcattg ggaataatac cttgtaaggc atctggctta tgttatcttc ctttaaatgg241 tattgtttta tttcttgtta aagaggatta tttctgtttg aacttagtca aatgtggtcc301 ataggctggc tatttttgta aatgaagctt tattggatat agccatgccc atttgtttaa361 tattaatata ttatctggct tcttttgtgc tataata<210>51<211>535<212>DNA<213>Homo sapiens
<400>511 aggttttttt ttttttaata tttttaatgt ttaatagtta aaatttttta acaatttaac61 tttaaaaagg tcacacattt tctgatccag caatgcccca atcagattgt ttcattttat121 tattattatc aacactgtcc cctttttggc acctgtaaaa tagttccttt cgggagtttg181 gagccaggcc aggcaccgtc ggtgcatggg atgagatggg caggtttgga gctcctctgt241 ctagtgagga tcacggtctg cagagaaggg ttggcctccc cgtctcctat caaggcttaa301 agcaaggaga accatcccaa atttggttcc ttttccccta agtatcctta gaggcaatcc361 accctgtgac taggtgacta ggtgaaggac tgaggtccag aaaggagcta tcttaaacct421 ggaatcccat ttcctagtct gcagccttaa gcagttaccc tctcagacaa ctagccctct481 ccttcctccg catgaaaacc catggcttac agggatggtt gttgctttcc ctaaa<210>52<211>545<212>DNA<213>Homo sapiens<400>521 tttttttttt ttaattttga aaagactgtt gatgtcactg tggtttatta ggtaaataca61 aagtataggc tcttgcattt tttaaaaagc aaaaccaaag agattagaaa ccaagtacac121 atatcctcta gaagagaaac ataaatcagt tttaacaatt aggcacttaa aaatgtaaat181 gtaagacaac attatagaag tataaactat agttacactc ctaaattcct cctgaaatgt241 ttacaaacac aaaatcacaa gcatggaaaa caaatttcct ctttatcaaa aaaggaacct301 gaatttgaat ccaatgtgat aaaccaatga ttaaggtact ggtgggtagg aaaaaagagc361 ttctgaaatt tacatatttg attaaaatgt aagcatttca taaaactatc acaatagcat421 acagtagata tcataatgaa aaatatatgt caggaaaata atgaatctag acttaaaatt481 ttactttaca acaaggcact taacacattg aaacagtaca gtaaatttcc aaaagaatgc541 aattg<210>53<211>516<212>DNA<213>Homo sapiens<400>531 aaaacaaacg ttcttctttt aatacagcca ttgaaaagat gacacattaa gaaacatgta61 aacactgcag ctgcttaatt acaagttgca ctgcttgatt tagtacctat gcactcccag121 gtttggggac cttaagagag ggggacagga gcctcccctc actttcacag ttggatagag181 aaggcaagaa tgtgggtaga gtaagaaaga gaaaacagaa gacctggctt gtgcacagcg241 cctgagagaa tcaaagcaaa ttctgtcttc taagtcctcc cagaagaaag gtttaagatg301 ttaggaaggt tggaaaatga acgtgtgttg tcagttggga ggcaaaggaa ttgacaaatg361 cttctagtta gaatatcagc taggaaaagg agggatacag agcctgacct catgctctga421 aaaggcaagg aacccagaac ttgcctccct tatccactta agccccaacc ttgatcatca481 agggcagcgc atgggtgatc ctgttcccat gaggat
<210>54<211>571<212>DNA<213>Homo sapiens<400>541 gaatgatgct tttttttatg atcttaatta cattaaagga tttaaaaaat gccactgatc61 tcacagttta caatatccaa atcttcaaac ctgctggaag aagtccacag cacagcctgg121 aaattgcatc cgttgcattc tctcgtgcag ttaccttctg ccttgatatg tagtcagttc181 ttcctgaagg atggaagctc tcttttgcag aaaattaacc tgtgatttta gggaggaaat241 ggtgtcttca ggttcttgtc ttagggatgc tggcatcaat cctttcaatt ttgtttcata301 ttcttgtcgt atgtaagtta tctgttcctg tgactccaat tctttgtgtt gtaatttttt361 ctctgcacat cgcacctgat tagaacggtt ttctaattca tcttgtaaaa ccttgattgc421 ttggtcatta tctctaatca gctgcttctt ctcatcttca aacttttgtc taacatcctg481 gagccgcctt tctgcagcaa gctgctgctg gctgttctct tctttcagag aggaaatggt541 tgtctgaagt tctgctatga tctgtgaaga t<210>55<211>1711<212>DNA<213>Homo sapiens<400>551 tttttttttt taaatctaag ctattcccat ctcccccatt ctatgctggg gtgatatacc61 acaagaagtt acaggaacaa ctgctaaaag aattaaacat cttgggcctt tacacagctg121 ttgctatatc atggctttgg tgcatggtcg gtcacagatg ctgtcaagag gcttatgttt181 agttatcctt ttgcttcccc aacccccaca ttaaaggtct ccttcacctt ctctgtcctt241 tttgctacct cccttcttcc tgctccaagc tcccacaaac cagccttaat aaaagaggaa301 ggatcaaggc aacactccac atcagtcata tttcagggca gcttgatgtt tgtttgctaa361 tagatggttg gtattatcta accaataggg tgactccaag ttttaaaaaa cagcaagact421 aattcagaaa taatattatt tcttaatttt tttaaaaata gagatggggt ctcactttgt481 tgcctaggct ggtcttgaat gcctaggctc aagcaatcct cccaccttgt cctcccaaag541 tgctaggatt acaggcgtga gccattgcac ccgggctctc agaaatctta aaaaggattt601 agtttcattt aaaaataaca aacacagttc cccaaatctg aaatttagtt attgaaactg661 gaccatgttg tccatggaaa acatatctta ctttatacca gattttaaaa ttaacactgg721 tgtaagtgca gcctaaaatt cctctgagcc ccacttttac ataagaaaaa ttattgaata781 tgataaaggt agcatttcca atcagtgaga aaagataggt tattcaattt gtgttgggac841 tggtggatat atatctagga aaaaaaagta cagactctaa atattgaaag gtaaaactga901 aaacataaaa atatttgaag aaatcactgg aaaattattc tacaaactgc atggggaatg961 acagtctgtg ataatagtaa ccaagaacca tgaaagaata gattggtaac aattcagcta1021 cataaaaatc cctaaatttc tctatggcag aaaccatgaa acagataaat ttaccaaact1081 ggaaaaaatt ttcacaaatc atgtcacaaa gagctaattt tcttaaaata tataaaaaag1141 atctttcaaa gtgtcataca caaaacactg cttttcgaaa aacataagct cctacaaagc
1201 agtaagaaaa tgatgaacaa atagaaaaat gggcaaaggt gatgatttaa cagttcacag1261 agaaatacaa atggcttcta aaatatataa aaagatgccc aacttcgttc ataatatgaa1321 aaatgtaaat taagactaca ctaagataca atttttcaca gatctgtttt ctaaaaataa1381 aataagcttg agagagagcc tcttatctga attctgggac agcctgattt gcatgaggga1441 aataggcatc ctcctgcact gctggtggga gggtaaaggt acagtctcag tggaggacag1501 tttgctgtta cctctcagaa ttacaaatgc atgtgtttgt tttgatgtag caatttccca1561 tctaggaatt tattcttgaa agacagtcat acaagatttt cacaccagca ttatttgtaa1621 tagcaaaaag atgggaaatt acctaaatgc tcatatatag gggactgcta aattgtggaa1681 ttccatgcaa ttctaaaaaa aaaaaaaaaa a<210>56<211>1720<212>DNA<213>Homo sapiens<400>561 aatacttttg atcacttcca tttgcctgca aagccagaaa cttctctcaa gaagattata61 gctgagtatg taaaacaaaa tatatgtaca actgtaatac caacaagcac agtggtaagt121 tataacaaga tatacgaagt aatagaaatt aagaagtgag agtatcttca ggcgaaggca181 actgggaagc tggagctcaa agtttaaggc tgtgttaaaa cacaacctaa agttcccagt241 agaagcttgc tatagacttt tggtggctct gagaattccc ctggtcacct cagtgtgagg301 ggtgggtgat tcttaacctc tggtgggttt tgttgcagtt tagctgctgt ggagggattt361 cctacaagga ctggtctcag aacatgtatt tcaactgctc agaagacaac cccagtcgag421 agcgctgctc tgtgccttac tcctgttgct tgcctactcc tgaccaggca gtgatcaaca481 ctatgtgtgg ccaaggtatg caggcctttg actacttgga agctagcaaa gtcatctaca541 ccaatggctg tattgacgag ttggtcaact ggatacacag caacctattc ttacttggtg601 gtgtggctct aggcctggcc atcccccagc tggtaggaat tctgctgtcc cagatcctag661 tgaatcagat caaagatcag atcaagctac agctctacaa ccagcagcac cgggctgacc721 catggtactg agaatccatc ctgcacctcc tcaccatgga aactggcaag cctcataaac781 gaacagcagt gggtgctgaa agcagcacca aatggagatt tggattccag ccccccagtg841 acagcccagt gggaagaagc aaactccaga tgggcagaag gcagggtgca caggtggctc901 cagtctcagg aggatgcgcc tcctctcccc catcccagcc ctcagcattg tgccagagtg961 atacccttaa gtgtttgggt ttatgttttc agttttgttt gggaaacagc agttgcacag1021 agagttgggg gtactgctgc tgccttttca ccgaggcact gccaccacca gctctagcag1081 gggtgctcct gagcttggcg gacatactta gatcctaacg tgccagtgag acctggctgt1141 ggagagtagc actggcagcc ctgcctggac tccacttggc atgataccag ctccagaagg1201 gaagggagtg gagcaggcag tgaggagaga gcctgggggt cggctgggga cagccgtatg1261 tgctaggtag gagtggaggg agatatgttt accaaatgcc tgtcctgcca tcctcccagg1321 tagtcagagt gagctacatc ctgccccacc ttcatttcca tggaaacacg gcagctagga1381 cacggggtac aacagcagcc aaattcttcc ccacctccct tacttcgaaa aaaagtttgg1441 aaccctggtc cctatactct gcagtcagaa gtgggactga gccatacatg cccttgaatt1501 cctccctgtc tggccctccc tctccagcaa gcagggtttt ctttaacttg gcagtgtgca1561 gaggagaagt ggtaacaccc ccaccccatt cccctgcatc ggggctcagt attcctacag1621 ggtaagaggt aggaatcttg ctgggacgag gggagccaga agtggcaata aaagcgtgtt
1681 gacctggaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa<210>57<211>653<212>DNA<213>Homo sapiens<400>571 tttttttttt ttttttgcat acagaattat tttatttaac ttaaaccatg tagtacttta61 ctagaaaaaa gcagagtaag agaaactaac gttgccttag cttcagccat tcaaaataga121 cagtttcttt tttccattat gtaaagaatc cagagtatat cgcaataaca ggaataaatt181 cttacaacag aatatacaaa aacattttga aatttttttc atctactgat tttttatata241 aacaggattt tttaggaata atttatacac agaaagtcat tttatgtaac aaattggcca301 tgttattacc tttttttttc ttacttaaaa aaattttttt ttaacaagaa aactcagaaa361 atgcattatt tgcggtgcat ccattccatc ccgccttctg gtttgatttt ttttatccca421 gacaaaggga tacccagagg tagacaaact ctggcaaacc tctcacctca acctcactgg481 cttaaaaagc agacaggtgt tttcaccggg cgtctcttcc accggtggat gtgtgtgtgc541 aacgccaaac atccaaatga aaagttgaaa acaaaaccca aatagtttcc agatctttct601 cctatgtagc gcaaaaaccc caggtgtttc ccatgataga gatattgtgg agt<210>58<211>1037<212>DNA<213>Homo sapiens<400>581 ttatttchcc gabctccact ctggctccca ccccacaagc gtcagagcag gaaacaagct61 tggstgagat gcctcaggcc tggtaacctg aggtgtagag cacccagaag gaagggtaaa121 agcagggggc aaagcggtgg ccctcccttt ctgggggtca cttctgggct ggggccagct181 gaaacctgtg tccaagtagc tttcagggct ggccacaccc taagccttgc aaaagggcct241 cctgcaaggg ctggcccatg gggtccccac cttcccagcc agtgaggtta gcatggttag301 gagtccacat gtgtgcaagt gcttgtgtgg aggctcatgt atgcatgtgt gtatatgcaa361 agctgcacat gacaatgtgc atgccagtcc agagttagat gtacctatgc agttgccctc421 aagcgaaggg tcatatttgg aaacaaggat ggctctaaac atgtaagcgt gcatgtgggc481 atgtatgtat ctggggccta aggaggtggg gaagtgggtg ttggggtaag ggctggcctt541 cagggcattt gcagaaggag gagtgggtgg gagggaaagg ctgggcagag caggggaagg601 agtgaaagcc aggcaggaaa gtggaagaac aggagaagct catgtaatgg attaccctcc661 acaggattat gttccttgat tcctgagagt tttttctctt gattttaccc cctcagtcta721 tcactgcaag agaaagaggt agaaaagaca aacagaccac aaaagacaag aacccagaca781 tatagacaga cgcacctgtt gcatgtgcat gagccagagc ctgggagaga agagagagcg841 tgcaagagag agctcagagc aggcaggcag cccacccctg cagcagtgct gggctyactg901 gagccctgca gragtcagca gccctgtatg cactcctctg gtttgtcagg taacaggggt961 gcccgccccy tcatgatcag caccggkggt ggggagctgc ttaggtgctc aaryggtctg1021 accatgggkc tggtctc
权利要求
1.一类在人类肝脏中表达的表达序列标签的序列，其特征在于，它包括(a)SEQ ID No.1～SEQ ID No.58所示的序列；(b)SEQ ID No.1～SEQ ID No.58所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.58所示的序列中每条序列有至少70％同源性的序列；(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的表达序列标签的序列，其特征在于，所述序列包括具有SEQID No.1～SEQ ID No.58所示的序列。
3.一种探针分子，其特征在于，所述的探针分子含有权利要求1中所述的序列中约8-100个连续的核苷酸。
全文摘要
本发明公开了一类在人类肝脏中表达的表达序列标签的序列。利用本发明的在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物中发挥重要作用。
文档编号C12Q1/68GK1955296SQ200510030828
公开日2007年5月2日 申请日期2005年10月28日 优先权日2005年10月28日
发明者黄健, 韩泽广 申请人:上海人类基因组研究中心