混合并匹配tc蛋白质用于病虫害防治的制作方法

专利名称：混合并匹配tc蛋白质用于病虫害防治的制作方法
背景技术：
在作物损失和使这些害虫得到控制的代价方面，昆虫和其它害虫每年花费农民几十亿美元。在农业生产环境中由虫害引起的损失包括减少作物产量、降低作物质量和增加收获成本。对于蔬菜和水果栽培者、观赏花生产者，以及家庭园丁和户主，虫害也是一种负担。
耕作方法，例如轮作和施用高水平氮肥，已经部分解决了由农业害虫引起的问题。然而，农田利用方面的多种需求限制轮作的使用。此外，在一些地区轮作会中断一些昆虫的越冬特性。
因此，合成的化学杀虫剂最主要是取决于达到足够水平的控制。然而，合成的化学杀虫剂的使用具有几个缺点。例如，这些化学试剂能够不利地影响一些有益的昆虫。靶昆虫也已形成对一些化学杀虫剂的抗性。而且，下雨和杀虫剂施用装置不适当的校准能够导致较差的控制。杀虫剂的使用常常引起环境问题，例如当使用不当时土壤和水源的污染，并且残余物也能够保留在所处理的水果和蔬菜上。使用一些杀虫剂的工作也可使进行施用的人员处于危险中。关于杀虫剂使用的严格的新的限制和一些有效杀虫剂的去除可能限制了实际的控制损害和病害虫的选择。
用生物杀虫药代替合成的化学杀虫剂，或者将化学杀虫剂与生物杀虫药相组合能够降低环境中毒性化学药品的水平。一些目前成功使用的生物杀虫剂来自土壤微生物苏云金芽孢杆菌(Bacillus thuringiensis(B.t.))。然而绝大多数B.t.菌株不呈现杀虫活性，仅一些B.t.菌株产生对害虫例如昆虫具有高度毒性并且其毒性活性是特异的蛋白质。已经分离了编码δ-内毒素蛋白质的基因。其它芽孢杆菌属(Bacillus)物种也产生杀虫蛋白质。
已经产生了基于重组DNA的B.t.产物并且批准其进行使用。另外，随着遗传工程技术的使用，用于将这些毒素递送至农业环境的多种方法都是优选的。这些方法包括使用用毒素基因进行遗传工程化的植物以获得昆虫抗性和使用稳定化的完整微生物细胞作为毒素递送载体。因此，所分离的芽孢杆菌属毒素基因正变得具有商业价值。
B.t.蛋白质毒素最初制剂为可喷雾昆虫控制剂。B.t.技术相对更新的应用已经用于分离编码这些毒素的基因和用其转化植物。转基因植物随后产生毒素，从而提供昆虫控制。见Mycogen公司的美国专利号5,380,831；5,567,600；和5,567,862。转基因B.t.植物是非常有效的，并且预测将在一些作物和区域具有重要用途。
对于芽孢杆菌(和其它生物学)杀虫蛋白质的成功农业使用还存在一些障碍。某些昆虫能够耐受芽孢杆菌毒素的作用。迄今为止，已经证明诸如棉子象鼻虫、黑切根虫和美洲棉铃虫(Helicoverpa zea)，以及大部分物种的成虫对许多B.t.δ-内毒素没有显著的敏感性。
另一个潜在的障碍是昆虫B.t.毒素抗性的形成。B.t.植物广泛使用的潜势已经引起人们关注与传统可喷雾施用相比可能更快地产生抗性管理事项。虽然在实验室中一些昆虫已经筛选出可产生B.t.毒素抗性，但只有有菱形斑纹的蛾(菜蛾(Plutella xylostella))已经证明在田地环境中具有抗性(Ferre，J.和Van Rie，J.，Annu.Rev.Entomol.47501-533,2002)。
B.t.转基因植物技术中的抗性管理策略已经受到极大关注。已经建议几种策略保留有效使用苏云金芽孢杆菌毒素的能力。例如，这些策略包括高剂量保护，和交替或共同配置不同的毒素(McGaughey等(1998)，“B.t.抗性管理”，Nature Biotechnol 16144-146)，正如在天然细菌中的策略一样。
因此，为了有效地控制多种昆虫，仍然非常需要研发出能够在植物中表达的额外基因。除了继续设法发现新的B.t.毒素(由于已经发现了大量B.t.毒素，这将变得越来越困难)之外，将非常期望发现其它产生能够用于转基因植物策略的毒素的细菌源(不同于B.t.)。
相对更近的从光杆状菌属(Photorhabdus)/致病杆菌属(Xenorhabdus)细菌群中克隆杀虫毒素基因的努力呈现出对来自苏云金芽孢杆菌的毒素的潜在备选毒素。致病杆菌属在分类学上定义为肠杆菌科(Enterobacteriaceae)的一员，尽管其具有某些非典型的该科特征。例如，该属菌株是典型的硝酸盐还原作用阴性和过氧化氢酶阴性菌。只是在最近致病杆菌属才已经进行细分形成了第二个属，光杆状菌属，该属包括三个物种，Photorhabdus asymbiotica、Photorhabdus temperata和发光光杆状菌(Photorhabdus luminescens)。发光光杆状菌具有三个公认的亚种，发光光杆状菌akhurstii亚种、发光光杆状菌laumondii亚种和发光光杆状菌发光亚种(标准种)。(Fischer-Le Saux，M.，Viallard，V.，Brunel，B.，Normand，P.，Boemare，N.E.光杆状菌属属的名称多相分类和新分类群的建议P.luminescens subsp.luminescens subsp.nov.、P.luminescenssubsp.akhurstii subsp.nov.、P.luminescens subsp.laumondii subsp.nov.、P.temperata sp.nov.、P.temperata subsp.temperate subsp.nov.和P.asymbiotica sp.nov.，Int.J.Syst.Bacteriol.49；1645-1656，(1999))。该分化基于容易被技术人员所辨别的几个明显特征。这些差别包括如下DNA-DNA特性研究；过氧化氢酶活性表型的存在(光杆状菌属)或缺乏(致病杆菌属)；生物发光特性的存在(光杆状菌属)或缺乏(致病杆菌属)；线虫宿主的科(因为致病杆菌属发现于斯氏线虫科(Steinernematidae)而光杆状菌属发现于异小杆线虫科(Heterorhabditidae))；以及可比较的细胞脂肪酸分析(Janse等人，1990，Lett.Appl.Microbiol.10，131-135；Suzuki等人，1990，J.Gen.Appl.Microbiol.，36，393-401)。此外，最近集中于16SrRNA基因的序列(Rainey等人，1995，Int.J Syst.Bacteriol.，45，379-381)和限制性分析(Brunel等人，1997，App.Environ.Micro.，63，574-580)的分子研究也支持这两个属的分开。
对于致病杆菌属所预期的特性如下革兰氏染色阴性杆菌、白色到黄/棕色菌落色素沉着、包涵体的存在、过氧化氢酶的缺乏、还原硝酸盐能力的缺乏、生物发光的缺乏、从培养基摄入染料的能力、阳性明胶水解作用、在肠杆菌科选择培养基上生长、生长温度低于37℃、在厌氧条件下生存和游动性。
目前，细菌致病杆菌属包括4个公认的物种，嗜线虫致病杆菌(Xenorhabdus nematophilus)、波氏致病杆菌(Xenorhabdus poinarii)、伯氏致病杆菌(Xenorhabdus bovienii)和贝氏致病杆菌(Xenorhabdusbeddingii)(Brunel等人，1997，App.Environ.Micro.，63，574-580)。在文献中已经叙述了多种相关菌株(例如Akhurst和Boemare 1988 J.Gen.Microbiol.，134，1835-1845；Boemare等人，1993 Int.J.Syst.Bacteriol.43，249-255页；Putz等人，1990，Appl.Environ.Microbiol.，56，181-186，Brunel等人，1997，App.Environ.Micro.，63，574-580，Rainey等人，1995，Int.J.Syst.Bacteriol.，45,379-381)。
光杆状菌属和致病杆菌属的种是与通过昆虫治病性和共生性与土壤线虫相关的革兰阴性细菌。这些细菌发现于侵袭和杀死昆虫的昆虫致病性线虫的肠道内。当线虫侵袭昆虫宿主时，细菌被释放入昆虫血腔内(开放循环系统)，并且细菌和线虫都进行多轮复制；昆虫宿主一般死亡。这些细菌能够能够离开其线虫宿主进行培养。关于这些细菌更加详细的论述，参见Forst和Nealson，60Microbiol.Rev.1(1996)，21-43页。不幸的是，如在大量文章中所报道，当注射入昆虫幼虫时细菌仅具有杀虫活性并且当口服递送时细菌不呈现生物学活性。
致病杆菌属和光杆状菌属细菌将多种物质分泌入培养基中。参见R.H.ffrench-Constant等人，66 AEMNo.8，3310-3329页(2000年8月)，综述了多种参与光杆状菌属昆虫毒性的因子。
已经难于有效地开发线虫或其细菌共生生物的杀虫特性。因此，来自具有口服活性的光杆状菌属/致病杆菌属细菌的蛋白质因子是令人期望的，以至于由其产生的产物能够制剂成可喷雾杀虫剂，或者编码所述蛋白质因子的基因能够进行分离并用于产生转基因植物。
在从发光光杆状菌和嗜线虫致病杆菌克隆编码杀虫毒素的基因方面已经取得实质性进展。首先检测了来自发光光杆状菌的毒素-复合体编码基因。参见WO 98/08932。最近更是从嗜线虫致病杆菌克隆了相似基因。参见，例如，Morgan等人，Applied and Environmental Microbiology 2001，672062-69。“相似性”的程度在下面进行更加详细地论述。
WO 95/00647涉及使用致病杆菌属蛋白质毒素控制昆虫，但是该专利不包括口服有效毒素。WO 98/08388涉及来自致病杆菌属的口服施用杀虫剂因子。美国专利号6,048,838涉及具有口服活性、从致病杆菌属物种和菌株可获得的蛋白质毒素/毒素复合体。在光杆状菌属spp中已经鉴定了4种不同的毒素复合体(TCs)-Tca、Tcb、Tcc和Ted。这些毒素复合体的每一种作为单体或者作为二聚体在天然琼脂糖凝胶上进行分离，但是在变性凝胶上的分离显示每种复合体由25-280kDa之间范围内的种类组成。来自光杆状菌属的编码典型TCs的ORF及蛋白酶切割位点(垂直箭头)图解显示于图7。也参见R.H.ffrench-Constant和Bowen，57Cell.Mol.Life Sci.828-833(2000)。
使用DNA探针并且使用针对毒素产生的单克隆和/或多克隆抗体筛选了P.luininescens基因组文库。克隆了4个tc基因座tca、tcb、tcc和tcd。tca基因座是从相同DNA链转录的三个开放阅读框(ORFs)tcaA、tcaB和tcaC的预测的操纵子，并具有以相反方向转录的较小终止ORF(tcaZ)。tcc基因座也包括三个推测以相同方向转录的ORF(tccA，tccB和tccC)。tcb基因座是一个大的ORF(tcbA)，并且tcd基因座包括2个ORF(tcdA和tcdB)；tcbA和tcdA，每个大约7.5kb，编码大的昆虫毒素。已经确定许多这些基因的产物被蛋白酶切割。例如，TcbA和TcdA切割成三个片段，命名为i、ii和iii(例如TcbAi、TcbAii和TcbAiii)。tca和tcc ORF的产物也被切割。参见图7。也参见R.H.ffrench-Constant和D.J.Bowen，Current Opinionsin Microbiology，1999，12284-288。
如WO 98/08932中所报道，来自光杆状菌属属的蛋白质毒素已经显示具有口服的抗昆虫毒性。例如，由发光光杆状菌(W-14)产生的毒素复合体已经显示包含10到14种蛋白质，并且已知这些蛋白质是通过表达来自4个不同基因组区域的基因tea、tcb、tcc和tcd所产生的。WO 98/08932公开了多种天然毒素基因的核苷酸序列。
Tca毒素复合体的生物学测定显示当经口施用时它们对于第一龄期的番茄天蛾幼虫(烟草天蛾(Manduca sexta))是高毒性的(LD50为每平方厘米875ng人工饵料)。R.H.ffrench-Constant和Bowen 1999。当Tca剂量低至40ng/cm2时摄食受到抑制。对于基于摩尔质量所给定的高度预测的Tca分子量，发光光杆状菌毒素是高度活性的，并且相对较少数分子表现出对于产生毒性效应是必需的。R.H.ffrench-Constant和Bowen，Current Opinions inMicriobiology，1999，12284-288。
这4个基因座与GenBank中已知功能的任意序列均不显示总体的相似性。序列同一性的区域引起一些建议，即这些蛋白质(TcaC和TccA)可以通过攻击昆虫血细胞而克服昆虫免疫性。R.H.ffrench-Constant和Bowen，Current Opinions in Microbiology，1999，12284-288。
TcaB、TcbA和TcdA彼此相互比较，全部显示氨基酸保守区(～50％同一性)紧密围绕其预测的蛋白质切割位点。3种不同的Tc蛋白质之间的该保守性表明它们可以全部通过相同或相似的蛋白酶进行加工。TcbA和TcdA也具有～50％同一性，以及相似的羧基-和氨基-末端预测切割模式。因此可以假定这些蛋白质可能是彼此的同系物(在某种程度上)。而且，TcbA和TcdA相似的大尺寸和两种毒素均作用于昆虫肠道的事实，也可以暗示相似的作用方式。R.H.ffrench-Constant和Bowen，Current Opinionsin Microbiology，1999，12284-288。
缺失/敲除研究表明tca和tcd基因座的产物负责多数对鳞翅类昆虫的口服毒性。缺失tca或者tcd基因极大地降低针对烟草天蛾的口服活性。也就是说，tca和tcd基因座的产物其自身是口服鳞翅类毒素；其组合的效果有助于绝大多数分泌的口服活性。R.H.ffrench-Constant和D.J.Bowen，57Cell.Mol.Life.Sci.831(2000)。有趣地是，单独缺失tcb或者tcc基因座的任何一个也会降低死亡率，这表明可能存在不同基因产物之间的复合体相互作用。因此，tca基因座的产物可以增强tcd产物的毒性。另外，tcd产物可以调节tca产物的毒性并且可能调节其它复合体。值得注意的是上述涉及到针对单一昆虫物种的口服活性，而tcb或tcc基因座可以产生针对其它类型昆虫具有更高活性(或者通过直接注射入昆虫血腔—当通过细菌体内分泌时正常的递送路线—具有活性)的毒素。R.H.ffrench-Constant和Bowen，Current Opinions in Microbiology，1999，12284-288。
昆虫中肠的上皮细胞含有柱状(结构)和杯状(分泌)细胞。M.sexta对tca产物的摄入导致柱状细胞顶端膨胀和大细胞质囊泡的起泡，这导致最终将小囊泡中的细胞核挤入肠腔。杯状细胞显然也受到相同方式的影响。口服递送或者注射之后tca的产物作用于昆虫中肠。R.H.french-Constant和D.J.Bowen，Current Opinions in Microbiology，1999，12284-288。纯化的tca产物已经显示出针对烟草天蛾的口服毒性(LD50为875ng/cm2)。R.H.ffrench-Constant和D.J.Bowen，57Cell.Mol.Life Sci.828-833(2000)。
WO 99/42589和美国专利号6,281,413公开了来自发光光杆状菌的TC-样ORF。WO 00/30453和WO 00/42855公开了来自致病杆菌属的TC-样蛋白质。WO 99/03328和WO 99/54472(和美国专利号6,174,860和6,277,823)涉及来自致病杆菌属和光杆状菌属的其它毒素。
WO 01/11029和美国专利号6,590,142B1公开了编码TcdA和TcbA并且具有已经从天然基因进行改变使得其与植物基因更加相似的碱基组合物的核苷酸序列。还公开了表达毒素A和毒素B的转基因植物。这些参考文献还公开了发光光杆状菌W-14株(ATCC 55397；保藏于1993年3月5日)和许多其它菌株。
在从发光光杆状菌(W-14)分离的分别的毒素中，那些命名为毒素A和毒素B的毒素已经成为所关注的一些研究的主题，这些研究是关于这些毒素针对目的靶昆虫物种(例如玉米根虫)的活性的。毒素A包括2个不同的亚基。天然基因tcdA编码前毒素TcdA。如通过质谱测定法所确定的那样，TcdA由一种或多种蛋白酶进行加工以提供毒素A。更加具体而言，TcdA是大约282.9kDa的蛋白质(2516aa)，其进行加工以提供TcdAi(最开始的88个氨基酸)、TcdAii(接下来的1849aa；由tcdA的核苷酸265-5811编码的大约208.2kDa的蛋白质)和TcdAiii，大约63.5kDa(579aa)的蛋白质(由tcdA的核苷酸5812-7551编码)。TcdAii和TcdAiii表现出组装成二聚体(可能由TcdAi辅助形成)，并且该二聚体再组装成4个二聚体的四聚体。毒素B同样来源于TcbA。
然而，目前还没有了解TC蛋白质彼此之间确切的分子相互作用及其作用机制，例如，已知光杆状菌属的Tca毒素复合体对烟草天蛾是具有毒性的。此外，已知一些TC蛋白质具有“独特(stand-alone)”杀虫活性，而其它TC蛋白质已知能够赋予或增强独特毒素的活性。已知单独的TcdA蛋白质针对烟草天蛾是有活性的，但与TcdB和TccC一起时，能够用于增强TcdA的活性。Waterfield，N.等人，Appl.Environ.Microbiol.2001，675017-5024。TcbA(仅有的唯一一种Tcb蛋白质)是另一种来自光杆状菌属的独特毒素。该毒素(TcbA)的活性也能够通过TcdB及TccC-样蛋白质增强。
美国专利申请20020078478提供来自发光光杆状菌W-14tcd基因组区域的两个增强子基因tcdB2和tccC2的序列。在其中表明了，与当tcdA在此类异源宿主中单独表达时所获得的毒性相比，tcdB和tccC1与tcdA在异源宿主中共表达会导致增强水平的口服昆虫毒性。tcdB和tccC1与tcdA或tcbA共表达会提供增强了的口服昆虫活性。
如在下面图表中所指出，TccA与TcdA的N末端具有一定水平的同源性，并且TccB与TcdA的C末端具有一定水平的同源性。TccA和TccB对于某些测试昆虫的活性比TcdA更低。来自光杆状菌属菌株W-14的TccA和TccB称为“毒素D”。在下面还指出了“毒素A”(TcdA)、“毒素B”(TcbA)和“毒素C”(TcaA和TcaB)。
而且，TcaA与TccA具有一定水平同源性并且同样与TcdA的N末端也具有一定水平的同源性。此外，TcaB与TccB具有一定水平同源性并且同样与TcdA的N末端也具有一定水平的同源性。
TccA和TcaA具有相似的大小，正如TccB和TcaB一样。TcdB与TcaC具有显著水平的相似性(在序列和大小方面)。
相对更新的致力于嗜线虫致病杆菌的克隆也表明已经鉴定出与发光光杆状菌tc基因座具有同源性的新的杀虫毒素基因，参见，例如，WO98/08388和Morgan等人，Applied and Environnaental Microbiology2001，672062-69。在R.H.ffrench-Constant和D.J.Bowen，CurrentOpinions in Microbiology，1999，12284-288中，粘粒克隆用于直接筛选针对另一种鳞翅类欧洲粉蝶(Pieris brassicae)的口服毒性。对一种口服毒素的粘粒克隆进行了测序。对该粘粒序列的分析表明有五个不同的ORF与光杆状菌属的tc基因具有相似性；orf2和orf5都与tcbA和tcdA具有一定水平的序列相关性，而orf1与tccB相似，orf3与tccC相似并且orf4与tcaC相似。大量这些预测的ORF也共同享有在发光光杆状菌中证明的推定的切割位点，这表明有活性的毒素还可以进行蛋白水解加工。
从这些致病基因可能的起源来看，在这两种不同的细菌中具有一些相似性的毒素编码基因座的发现是非常有趣的。嗜线虫致病杆菌粘粒也表现出含有转位酶样序列，该序列的存在可以表明这些基因座能够水平转移至不同细菌菌株或物种之间的潜能。此类转移事件的范围也可以解释两种不同细菌中tc操纵子明显不同的基因组组成。另外，仅嗜线虫致病杆菌和发光光杆状菌菌株的亚群显示出对烟草天蛾具有毒性，这表明不同菌株缺乏tc基因或者它们携带不同的tc基因补充物(compliment)。对这些细菌物种内和之间的菌株和毒素种系发生进行详细分析有助于阐明毒素基因可能的起源并阐明它们如何维持于不同的细菌种群中。R.H.ffrench-Constant和Bowen，Current Opinions in Microbiology，1999，12284-288。
存在五种典型的致病杆菌属TC蛋白质XptA1、XptA2、XptB1、XptC1和XptD1。XptA1是“独特”毒素。XptA2是另一种来自致病杆菌属具有独特毒素活性的TC蛋白质。XptB1和XptC1是能够增强任一种(或两种)XptA毒素的活性的增效剂。XptD1与TccB具有一定水平同源性。XptC1与光杆状菌属TcaC具有一定水平相似性。致病杆菌属的XptA2蛋白质与光杆状菌属TcdA蛋白质具有一定程度相似性。XptB1与光杆状菌属TccC具有一定水平相似性。
最近已经对来自其它昆虫相关的细菌诸如嗜线虫沙雷菌(Serratiaentomophila)，一种昆虫病原体，的TC蛋白质和基因进行了叙述。有学者发现假单胞菌属(Pseudomonas)物种具有增效剂。Waterfield等人，TRENDS in Microbiology，Vol.9，No.4，April 2001。
通过特定的rRNA和表型特征可以将类芽孢杆菌属(Paenibacillus)的细菌与其它细菌进行区别(C.Ash等人(1993)，“利用PCR探针测试分子鉴定3种杆菌的rRNA(Ash、Farrow、Wallbanks和Collins)建立新的类芽孢杆菌属的建议”，Antonie vaya Leeuwenhoek 64253-260)。已知该属中的一些物种对于蜜蜂(幼虫类芽孢杆菌(Paenibacillus larvae))和金龟子甲虫幼虫(P.popilliae和P.lentimorbus)是致病的。幼虫类芽孢杆菌、P.popilliae和P.lentimorbus被认为是与金龟子甲虫乳白病有关的专性昆虫病原体(D.P.Stahly等人(1992)，“芽孢杆菌属昆虫病原体”，1697-1745页，于A.Balows等人编著，原核生物，第二版，第2卷，Springer-Verlag，New York，NY)。
在Paenibacillus popilliae和Paenibacillus lentimorbus菌株中已经鉴定了晶体蛋白质Cry18。Cry18具有金龟子和幼虫毒性，并且与Cry2蛋白质具有大约40％同一性(Zhang等人，1997；Harrison等人，2000)。最近已经在类芽孢杆菌属中发现了TC蛋白质和鳞翅类毒素Cry蛋白质。参见美国系列号60/392,633(Bintrim等人)，2002年6月28日申请。在该类芽孢杆菌属菌株中发现了6种TC蛋白质ORF。ORF3和ORF1显示出分别与TcaA具有一定水平同源性。ORF4和ORF2显示出与TcaB具有一定水平同源性。ORF5表现出是TcaC-样增效剂，并且ORF6与TccC增效剂具有同源性。
尽管发现了一些致病杆菌属TC蛋白质与一些光杆状菌属TC蛋白质相对应(具有相似的功能和一定水平序列同源性)，所给定的光杆状菌属蛋白质与“相对应的”致病杆菌属蛋白质具有仅大约40％的序列同一性。下面图表说明了四种“独特的”毒素
(对于更加全面的综述，参见，例如，Morgan等人，“来自嗜线虫致病杆菌PMFI296的杀虫剂基因的序列分析”，第67卷，Applied andEnvironmental Microbiology，2001年5月，2062-2069页)。当将最新新发现的来自类芽孢杆菌属的TC蛋白质(那些蛋白质和发现是共同待决的美国系列号60/392,633的主题)与其致病杆菌属和光杆状菌属“相应物”相比较时，也发现了这种近似程度的序列相关性。
虽然光杆状菌属毒素已经成功地应用，并且致病杆菌属毒素也已经成功地应用(除了光杆状菌属毒素外)，但目前为止还没有提出或阐明用来自其它生物源(例如致病杆菌属)的一种或多种TC蛋白质增效剂增强来自于这些生物源(诸如光杆状菌属)之一的TC蛋白质毒素的活性。
发明概述本发明的主题涉及令人惊讶的发现，即毒素复合体(TC)蛋白质(从诸如致病杆菌属、光杆状菌属和类芽孢杆菌属的生物体获得)能够彼此可替换的进行使用。作为本领域的技术人员将认识到本公开的益处，这具有广泛的意义并且扩大了各种类型TC蛋白质目前已经认识到的用途的范围。这在以前没有考虑到，并且这在以前将认为是不可能的，例如，尤其在序列水平来自光杆状菌属的TC蛋白质与致病杆菌属和类芽孢杆菌属的“相应”TC蛋白质相比的高水平的序列差异。
在本发明特别优选的实施方案中，通过来自不同来源生物体的一种或多种TC蛋白质“增效剂”增强“独特”TC蛋白质(例如，来自光杆状菌属、致病杆菌属或类芽孢杆菌属)的毒性。本发明提供给本领域技术人员许多令人惊讶的优点。最重要的优点之一是本领域的技术人员现在将能够使用单一一对增效剂增强独特致病杆菌属蛋白质毒素和独特光杆状菌属蛋白质毒素的活性。(如本领域的技术人员所知，致病杆菌属毒素蛋白质更加倾向于控制鳞翅类昆虫而光杆状菌属毒素蛋白质更加倾向于控制鞘翅类昆虫。)这会降低需要由不同的转基因植物和/或植物细胞共表达的基因(和转化事件)的量，以获得对更广谱的靶害虫的有效控制。
从另一方面说明，本发明涉及致病杆菌属TC蛋白质能够用于增强光杆状菌属TC蛋白质的活性并且反之亦然。同样，并且也是令人惊奇的，发现了来自类芽孢杆菌属的TC蛋白质能够用于替换致病杆菌属/光杆状菌属的TC蛋白质，并且反之亦然。此外，没有预计到来自这些趋异生物体的蛋白质能彼此兼容；这在以前没有提出或证明。本发明尤其令人惊奇之处在于阐明了致病杆菌属、光杆状菌属和类芽孢杆菌属TC蛋白质(以及来自其它属的TC蛋白质)之间显著的差别，尽管这些蛋白质也具有一些共同的特性。
此处还公开了异源TC蛋白质的某些优选的组合。
本发明的一些目的、优点和特点对于本领域受益于本公开的技术人员将是显而易见的。
附图简述

图1显示pDAB2097中所鉴定的ORF的方向。
图2显示表达载体质粒pBT-TcdA。
图3显示表达载体质粒pET280载体。
图4显示表达载体质粒pCot-3。
图5是pBT构建的图解式图。
图6是pET/pCot构建的图解式图。
图7显示来自光杆状菌属的TC操纵子。
序列简述SEQ ID NO1是毒素XwiA220kDa蛋白质(XptA2Wi)的N-末端。
SEQ ID NO2是毒素XwiA纯化的毒素(XptA2Wi)的内部肽。
SEQ ID NO3是毒素XwiA纯化的毒素(XptA2Wi)的内部肽。
SEQ ID NO4是毒素XwiA纯化的毒素(XptA2Wi)的内部肽。
5EQ ID NO5是毒素XwiA纯化的毒素(XptA2Wi)的内部肽。
SEQ ID NO6是pDAB2097粘粒插入部分39,005bp。
SEQ ID NO7是pDAB2097粘粒ORF1SEQ ID NO6的核苷酸1-1，533。
SEQ ID NO8是由pDAB2097粘粒ORF1推导出的蛋白质511aa。
SEQ ID NO9是pDAB2097粘粒ORF2(XptD1Wi)SEQ ID NO6的核苷酸1,543-5,715。
SEQ ID NO10是由pDAB2097粘粒ORF2推导出的蛋白质1,391aa。
SEQ ID NO11是pDAB2097粘粒ORF3SEQ ID NO6的核苷酸5,764-7,707。
SEQ ID NO12是由pDAB2097粘粒ORF3推导出的蛋白质648aa。
SEQ ID NO13是pDAB2097粘粒ORF4(XptA1 wi)SEQ ID NO6的核苷酸10,709-18,277。
SEQ ID NO14是由pDAB2097粘粒ORF4推导出的蛋白质2,523aa。
SEQ ID NO15是pDAB2097粘粒ORF5(xptB1wi)SEQ ID NO6的核苷酸18,383-21,430(C)。
SEQ ID NO16是由pDAB2097粘粒ORF5推导出的蛋白质1,016aa。
SEQ ID NO17是pDAB2097粘粒ORF6(xptC1wi)SEQ ID NO6的核苷酸21,487-25,965(C)。
SEQ ID NO18是由pDAB2097粘粒ORF6推导出的蛋白质1,493aa。
SEQ ID NO19是pDAB2097粘粒ORF7(xptA2wi)SEQ ID NO6的核苷酸26,021-33,634(C)。
SEQ ID NO20是由pDAB2097粘粒ORF7推导出的蛋白质2,538aa。
SEQ ID NO21是来自GENBANK登录号AF188483的TcdA基因和蛋白质序列。
SEQ ID NO22是来自GENBANK登录号AF346500的TcdB1基因和蛋白质序列。
SEQ ID NO23是用于从质粒pBC-AS4扩增TcdB1序列的正向引物。
SEQ ID NO24是用于从质粒pBC-AS4扩增TcdB1序列的反向引物。
SEQ ID NO25是来自GENBANK登录号AAC38630.1的TccC1的基因和蛋白质序列。
SEQ ID NO26是用于从质粒pBC KS+载体扩增TccC1的正向引物。
SEQ ID NO27是用于从质粒pBC KS+载体扩增TccC1的反向引物。
SEQ ID NO28是用于扩增xptA2wi的正向引物。
SEQ ID NO29是用于扩增xptA2wi的反向引物。
SEQ ID NO30是用于扩增xptC1wi的正向引物。
SEQ ID NO31是用于扩增xptC1wi的反向引物。
SEQ ID NO32是用于扩增xptB1wi的正向引物。
SEQ ID NO33是用于扩增xptB1wi的反向引物。
SEQ ID NO34是来自嗜线虫致病杆菌Xwi的XptA2wi蛋白质的氨基酸序列。
SEQ ID NO35是来自类芽孢杆菌DAS1529株的ORF3的核酸序列，该序列编码tcaA-样蛋白质。
SEQ ID NO36是由类芽孢杆菌属ORF3编码的氨基酸序列。
SEQ ID NO37是来自类芽孢杆菌DAS1529株的ORF4的核酸序列，该序列编码tcaB-样蛋白质。
SEQ ID NO38是由类芽孢杆菌属ORF4编码的氨基酸序列。
SEQ ID NO39是来自类芽孢杆菌DAS1529株的ORF5的核酸序列，该序列编码tcaC-样蛋白质(pptB11529)。
SEQ ID NO40是由类芽孢杆菌属ORF5编码的氨基酸序列(PptB11529)。
SEQ ID NO41是来自类芽孢杆菌DAS1529株的ORF6(短)的核酸序列，该序列编码tccC-样蛋白质(pptC11529S)。
SEQ ID NO42是由类芽孢杆菌属(短)ORF6编码的蛋白质序列(PptC11529S)。
SEQ ID NO43是由SEQ ID NO55的类芽孢杆菌属ORF6(长)编码的另一个(长)蛋白质序列(PptC11529L)。
SEQ ID NO44是编码TcdB2的核苷酸序列。
SEQ ID NO45是TcdB2蛋白质的氨基酸序列。
SEQ ID NO46是TccC3的核苷酸序列。
SEQ ID NO47是TccC3蛋白质的氨基酸序列。
SEQ ID NO48是天然xptB1xb编码区(4521个碱基)。
SEQ ID NO49是由SEQ ID NO48所编码的天然XptB1xb蛋白质(1506个氨基酸)。
SEQ ID NO50是天然XptC1xb编码区(2889个碱基)。
SEQ ID NO51是由SEQ ID NO50所编码的天然XptC1xb蛋白质(962个氨基酸)。
SEQ ID NO52是包含天然xptB1xb编码区的表达质粒pDAB6031的Xba I至XhoI片段(4595个碱基)，其中碱基40至4557编码SEQ IDNO49的蛋白质。
SEQ ID NO53是包含天然xptC1xb编码区的表达质粒pDAB6032的Xba I至Xho I片段(2947个碱基)，其中碱基40至2925编码SEQID NO51的蛋白质。
SEQ ID NO54是包含天然xptB1xb和天然xptC1xb编码区的表达质粒pDAB6033的Xba I至Xho I片段(7508个碱基)，其中碱基40至4557编码SEQ ID NO49的蛋白质，并且碱基4601至7486编码SEQ ID NO51的蛋白质。
SEQ ID NO55是来自类芽孢杆菌DAS1529株的ORF6(长；pptC11529L)的核酸序列，该序列编码公开于SEQ ID NO43的tccC-样蛋白质(PptC11529L)。
SEQ ID NO56是来自GENBANK登录号AF346497.1的TcaC的基因和蛋白质序列。
SEQ ID NO57是来自GENBANK登录号AF346500.2的TccC5的基因和蛋白质序列。
SEQ ID NO58是来自GENBANK登录号AAL18492的TccC2的蛋白质序列。
SEQ ID NO59显示TcbAw-14蛋白质的氨基酸序列。
SEQ ID NO60显示SepB蛋白质的氨基酸序列。
SEQ ID NO61显示SepC蛋白质的氨基酸序列。
SEQ ID NO62显示TcdA2w-14蛋白质的氨基酸序列。
SEQ ID NO63显示TcdA4w-14蛋白质的氨基酸序列。
SEQ ID NO64显示TccC4W-14蛋白质的氨基酸序列。
发明详述本发明涉及毒素复合体(TC)蛋白质的新用途，这些TC蛋白质可从诸如致病杆菌属、光杆状菌属和类芽孢杆菌属的生物体获得。如下面所讨论，一种或多种TC增效剂用于增强TC毒素蛋白质的活性，这不同于天然情况下一种或两种增效剂增强的TC毒素蛋白质。作为本领域的技术人员将认识到本公开的益处，这具有广泛的意义并且扩大了多种类型TC蛋白质目前已经认识到的用途的范围。
已经知道一些TC蛋白质具有“独特”杀虫活性，并且其它TC蛋白质已知可以增强由相同特定生物体所产生的该独特毒素的活性。在本发明特别优选的实施方案中，“独特”TC蛋白质(例如，来自光杆状菌属、致病杆菌属或类芽孢杆菌属)的毒性通过来自不同属源生物体的一种或多种TC蛋白质“增效剂”增强。
存在三种主要类型的TC蛋白质。如此处所提及，A类蛋白质(“蛋白质A”)是独特的毒素。天然A类蛋白质大约280kDa。
B类蛋白质(“蛋白质B”)和C类蛋白质(“蛋白质C”)增强A类蛋白质的毒性。如此处提及所使用的，天然B类蛋白质大约170kDa，并且天然C类蛋白质大约112kDa。
A类蛋白质的例子是TcbA、TcdA、XptA1和XptA2。B类蛋白质的例子是TcaC、TcdB、XptB1xb和XptC1wi。C类蛋白质的例子是TccC、XptC1xb和XptB1wi。
目前仍然不知道毒性和活性增强作用的确切机制，但是作用的确切机制也不是重要的。重要的是靶昆虫吃或者摄入A、B和C类蛋白质。
已知仅用TcdA蛋白质是对烟草天蛾具有活性的。已知TcdB1和TccC一起能够用于增强TcdA的活性。TcbA是另一种独特的光杆状菌属毒素。目前本领域中所考虑的TC蛋白质的一种组合是TcaC(或TcdB)和TccC(作为增效剂)共同与TcdA或TcbA组合。在致病杆菌属中也是同样的，已知XptB1和XptC1增强XptA1或XptA2的活性，其中后者各自是“独特”毒素。
虽然(TcbA或TcdA)+(TcaC+TccC)复合体似乎显示出与(XptA1或XptA2)+(XptC2+XptB1)复合体相似的排列，但每种光杆状菌属成分与“相应”致病杆菌属成分仅具有大约40％(近似)的序列同一性。来自类芽孢杆菌属的独特TC蛋白质也与“相应”光杆状菌属和致病杆菌属TC蛋白质(那些蛋白质和发现都是共同待决的美国申请系列号60/392,633的主题，Bintrim等，2002年6月28日申请)仅具有大约40％的序列同一性。
如此处所述，正是在本申请的内容中发现了致病杆菌属TC蛋白质能够用于增强光杆状菌属TC蛋白质的活性并且反之亦然。此处还令人惊讶地证明了类芽孢杆菌属TC蛋白质具有增强致病杆菌属(和光杆状菌属)TC毒素活性的潜能。这在以前是未提出和进行证明的，并且尤其令人惊讶的是在于阐明了致病杆菌属、光杆状菌属和类芽孢杆菌属TC蛋白质之间显著的不同。当然以前也没有预计到来自趋异生物体的不同蛋白质彼此互相兼容。
本发明能够以多种不同的方式实施。植物能够设计成产生两种类型A类蛋白质和单一一对增效剂(B和C蛋白质)。植物的每个细胞，或给定类型组织(例如根或叶)的每个细胞能够具有编码两种A蛋白质和B与C对的基因。
另外，植物的不同细胞仅能够产生这些蛋白质中的一种(或多种)。在这种情况下，当昆虫咬食并食入植物组织时，昆虫食入了产生第一种蛋白质A的细胞，另一种产生第二种蛋白质A的细胞，另一种产生B蛋白质的细胞，以及另一种产生C蛋白质的细胞。因此，产生本发明的两种A蛋白质、B蛋白质和C蛋白质的植物(不必由每一植物细胞均产生这四种蛋白质)将是重要的，从而当害虫食入植物组织时会食入全部这四种蛋白质。
除了转基因植物以外，在本发明的组合中，还有一些其它方式将蛋白质施用于靶害虫。喷射应用是本领域中已知的。几种或全部A、B和C蛋白质能够进行喷射应用(植物能够产生一种或多种蛋白质，并且可将其它蛋白质进行喷雾)。例如，土壤应用的多种类型的饵料颗粒也是本领域已知的，并且能够依照本发明进行使用。
此处显示了多种TC蛋白质的许多组合以令人惊讶的、新的方式起作用。例如，文中提出的一个实例显示TcdB1和TccC1用于增强XptA2针对谷物害虫幼虫的活性的用途。例如，文中所提出的另一个实例是XptB1与TcdB1一起增强TcdA针对谷物根虫的活性的用途。相类似地，并且也是令人惊讶地，进一步发现了来自类芽孢杆菌属的TC蛋白质能够用于增强TcdA-样和XptA2Xwi-样蛋白质的活性。文中所包括的一些例子如下
对于能够利用本公开的本领域技术人员，这些和其它组合的用途现在是显而易见的。
诸如TcbA、TcdA、XptA1和XptA2的独特毒素各自在大约280kDa的大小范围内。TcaC、TcdB1、TcdB2和XptC1每个大约170kDa。TccC1、TccC3和XptB1每个大约112kDa。因此，本发明优选的实施方案包括280-kDa型TC蛋白质毒素(如此处所述)与170-kDa类TC蛋白质(如此处所述)和112-kDa类TC蛋白质(如此处所述)的用途，其中所述的三类蛋白质中至少一种来自于源生物体(诸如光杆状菌属、致病杆菌属或类芽孢杆菌属)，该源生物体不同于一种或多种其它TC蛋白质所来源的源生物体的属。
本发明提供给本领域的技术人员许多令人惊奇的优点。其中最重要的优点是本领域的技术人员现在能够使用单一一对增效剂增强例如独特致病杆菌属蛋白质毒素以及例如独特光杆状菌属蛋白质毒素的活性。(如本领域的技术人员所知，致病杆菌属毒素蛋白质更预期倾向于控制鳞翅类昆虫，而光杆状菌属毒素蛋白质更预期倾向于控制鞘翅类昆虫。)这减少了需要通过转基因植物表达以获得对更广谱的靶害虫有效控制的基因(和转化事件)的数量。也就是说，不需要表达六个基因—两个毒素和两对增效剂—本发明允许仅表达四个基因—两个毒素和一对增效剂蛋白质。
因此，本发明包括共表达编码两种(或多种)不同独特TC蛋白质毒素的一条多核苷酸或多条多核苷酸，以及编码单一一对TC蛋白质增效剂—B类蛋白质和C类蛋白质—的一条多核苷酸或多条多核苷酸的转基因植物和/或转基因植物细胞，其中一种或两种所述增效剂来自与独特TC蛋白质毒素所来源的属不同属的细菌。因此，目前技术人员能够获得具有两种(或多种)能够被单一一对蛋白质增效剂(B类和C类蛋白质)增强的TC蛋白质毒素(A类蛋白质)。以前没有产生此类细胞的建议，并且肯定没有期望由所述细胞所产生的两种(或全部)此类毒素将具有足够水平的活性(由于如此处所报道的令人惊讶的增强作用)。作为术语在此处使用的TC蛋白质在本领域是已知的。此类蛋白质包括独特毒素和增效剂。已知产生TC蛋白质的细菌包括以下属的那些细菌光杆状菌属、致病杆菌属、类芽孢杆菌属、沙雷氏菌属(Serratia)和假单胞菌属。参见，例如，丁香假单胞菌(Pseudomonas syringae pv.syringae B728a)(GenBank登录号gi23470933和gi23472543)。根据本发明能够使用任一此类TC蛋白质。
独特(A类)毒素(此处作为术语使用)的例子包括来自光杆状菌属的TcbA和TcdA，以及来自致病杆菌属的XptA1和XptA2。该类毒素为大约280kDa。其它独特毒素的例子包括来自嗜线虫沙雷菌的SepA(GenBank登录号AAG09642.1)。例如，A类蛋白质可以是～230kDa(特别是如果被截短时)、～250-290kDa、～260-285kDa和～270kDa。
存在两种主要型或类的增效剂，此处作为术语进行使用。“B类”增效剂(文中有时称为增效剂1)的例子包括来自光杆状菌属的TcaC、TcdB1和TcdB2，来自致病杆菌属的XptC1，以及类芽孢杆菌属菌株DAS 1529ORF5的蛋白质产物。该类增效剂一般处于大约170kDa的大小范围内。其它～170kDa类增效剂的例子是来自嗜线虫沙雷菌的SepB(GenBank登录号AAG09643.1；这里显示为SEQ ID NO60)、来自丁香假单孢菌B728a的TcaC同系物(GenBank登录号gi23472544和gi23059431)和嗜线虫致病杆菌PO ORF268(由WO 20/004855图2的碱基258-1991所编码)。优选的～170kDa增效剂是TcdB2(SEQ ID NO44-45)。B类蛋白质可以是例如～130-180kDa、～140-170kDa、～150-165kDa和～155kDa。
“C类”增效剂(文中有时称为增效剂2)的例子包括来自光杆状菌属的TccC1和TccC3，来自致病杆菌属的Xpt B1以及类芽孢杆菌DAS1529株ORF6的蛋白质产物。该类增效剂一般处于大约112kDa的大小范围内。其它～112kDa类增效剂的例子是来自嗜线虫沙雷菌的SepC(GenBank登录号AAG09644.1；这里显示为SEQ ID NO61)，以及来自丁香假单孢菌B728a的TccC同系物(GenBank登录号gi23470227、gi23472546、gi23472540、gi23472541、gi23468542、gi23472545、gi23058175、gi23058176、gi23059433、gi23059435和gi23059432)。优选的～112kDa增效剂是TccC3(SEQ ID NO46-47)。C类蛋白质可以是例如～90-120kDa、～95-115kDa、～100-110kDa和～105-107kDa。
WO02/94867、美国专利申请20020078478和Waterfield等人(TRENDSin Microbiology Vol.10，No.12，Dec.2002，541-545页)公开了根据本发明能够进行使用的TC蛋白质。例如，Waterfield等人公开了tcdB2、tccC3、tccC5、tcdA2、tcdA3和tcdA4基因和蛋白质。在上面发明背景部分中所讨论的相关参考文献(和任意其它与TC蛋白质相关的参考文献)公开的任意相关TC蛋白质根据本发明也能够进行使用。
因此，本发明的一个实施方案包括产生一种、两种或多种类型独特TC蛋白质毒素和单一一对增效剂增效剂1和增效剂2(在上面或文中其它地方给出了这三种成分每一种的例子)的转基因植物或植物细胞，其中至少一种所述TC蛋白质来自与一种或多种其它TC蛋白质所来源的属不同的属的生物体。
应该很清楚本发明的例子包括这样的转基因植物或植物细胞，该转基因植物或植物细胞产生/共表达一种类型的光杆状菌属毒素(例如，TcbA或TcdA)、一种类型的致病杆菌属毒素(例如，XptA1或XptA2)和单一(一对并仅一对)一对增效剂蛋白质(例如，TcaC和TccC，不含XptC1或XptB1；或者XptC1和XptB1，不含TcaC或TccC；或者TcaC和类芽孢杆菌属ORF6，不含任意其它增效剂；或者TcdB和XptB1，不含任意其它增效剂；这些组合仅作为例证；许多其它组合对于将利用本公开的本领域技术人员是很清楚的)。根据本发明添加的增效剂能够用于增强异源毒素，但多种类型的增效剂对不是必需的。这是本发明非常令人惊讶的一个方面。
也很明显本发明能够以多种方式进行定义—而不是根据通过转基因植物或植物细胞所表达的产物进行定义。例如，本发明包括通过将一种或多种独特TC蛋白质毒素与单一一对增效剂共表达/共产生使得能够增强该种或多种独特TC蛋白质毒素的活性，其中一种或两种增效剂来自与TC蛋白质毒素所来源的生物体属不同的属的生物体。本发明还包括通过将一种或多种类型的TC蛋白质毒素与一对或多对增效剂一起(例如，TcbA和XptA1和XptC1和XptB1，可能不含TcaC和TccC)，包括产生该种蛋白质组合的细胞，投喂给害虫来控制昆虫(或类似)病虫害的方法，其中一种或两种增效剂来自与一种或两种独特毒素所来源的属不同的属的生物体。
在此之前没有考虑到此类安排或预期其具有活性。理解为何本结果是令人惊奇的一个方面是考虑到此处作为例证的一些蛋白质成分的序列相关性。例如，XptA2，来自致病杆菌属的独特毒素，与TcdA具有大约43％的序列同一性并且与TcbA具有大约41％的序列同一性。TcdA和TcbA各自是来自光杆状菌属的独特毒素。XptA1(另一种来自致病杆菌属的独特毒素)与TcdA和TcbA具有45％的同一性。
TcaC(光杆状菌属～170kDa增效剂)与XptC1(170kDa致病杆菌属增效剂)具有大约49％的序列同一性。TccC(～112kDa光杆状菌属增效剂)与XptB1(～112kDa致病杆菌属增效剂)具有大约48％的序列同一性。例如，在此之前，从未期望TcaC+TccC会增强与TcaC+TccC相关的天然“靶”仅具有40-45％序列同一性的蛋白质(XptA1或XptA2)的活性。(通过使用程序FASTA 6.0获得上面报道的数值并且来自Morgan等人“来自嗜线虫致病杆菌PMFI296的杀虫剂基因的序列分析”，第67卷，Applied and Environmental Microbiology，2001年5月，2062-2069页)。
根据本发明使用的成分的一些例子和其彼此之间的相关性，包括A类蛋白质
B类蛋白质
C类蛋白质
因此，所提到的TC蛋白质由其来源的细菌的属不是任意命名的细菌属。如上面图解说明，如此有助于定义一类TC蛋白质，该类蛋白质在彼此之间是相对保守的(例如由光杆状菌属物种和菌株产生的TC蛋白质的特定类型)，但是与其它来源于不同微生物属的“相应”TC蛋白质(例如由多种致病杆菌属物种和菌株产生的TC蛋白质)相对是非常不同的。
定义本发明每种TC蛋白质成分的另一种方法是通过所给定的蛋白质与所给定的毒素或增效剂的序列同一性的程度。此处提供了计算同一性分值的方法。因此，本发明特定的实施方案包括共产生与XptA2具有至少75％序列同一性的毒素、与TcdA或TcbA具有至少75％同一性的毒素、与TcdB1或TcdB2具有至少75％序列同一性的增效剂和与TccC1或TccC3具有至少75％序列同一性的增效剂的转基因植物或植物细胞。根据本发明的教导，其它TC蛋白质能够替换入上面的配方。另外，此处其它地方还提供了更加特定范围的同一性分值。
然而另一种定义本发明给定类型TC蛋白质成分的方法是通过编码该蛋白质的多核苷酸的杂交特性。本说明书自始至终都提供了关于此类“测试”和杂交(和洗涤)更加详细的信息。因此，根据本发明通过编码TC蛋白质的多核苷酸与给定“tc”基因杂交的能力能够定义所使用的TC蛋白质。
将该指导应用于特定实例，本发明XptA2-型毒素能够定义为由这样的多核苷酸所编码，其中编码所述XptA2-型毒素的核酸序列与SEQ ID NO19的xptA2基因杂交，其中在杂交和洗涤之后杂交维持在任意此处所述或所建议的此类条件下(例如此处所述的低、中度和高度严格杂交/洗涤条件的例子)。任意其它作为例证或所建议的TC蛋白质(包括增效剂或其它毒素)例如TcdB2、TccC3、TcdA和TcbA能够替代该定义中的XptA2。
因此，本发明包括共表达编码本发明TC蛋白质的多核苷酸的某些组合的转基因植物、转基因植物细胞或者细菌细胞。应该清楚本发明包括共表达两个毒素基因和一对增效剂的转基因植物或植物细胞。因此，本发明包括包含一条或多条编码如下面毒素对1、2、3或4所指出的一类毒素的多核苷酸的转基因植物或植物细胞，并且其中所述植物或植物细胞由编码一对增效剂的DNA组成，如下面所指出，该对增效剂选自显示于增效剂对1、2、3、4、5或6的增效剂分类中的蛋白质。以另一种方式进行叙述，所述植物或细胞由编码增效剂对1、2、3、4、5或6中的一种增效剂的多核苷酸部分组成，并且所述植物或细胞由编码所选增效剂对中的其它增效剂的另一多核苷酸部分组成。
植物或细胞能够包含编码额外TC蛋白质毒素的基因(例如，从而使细胞产生TcbA与TcdA，和/或XptA1与XptA2)，但是根据本发明优选的实施方案仅使用了一对增效剂。(当然，细胞或植物将产生多拷贝增效剂；关键是能够避免额外的转化事件。)本发明其它实施方案包括共表达独特蛋白质毒素和包含至少一种“异源”(所来源的细菌的属不是毒素所来源的生物体的属)TC蛋白质的单一(不多于一种)增效剂对。本发明还包括使用作为增效剂的一对TC蛋白质赋予TC蛋白质毒素增强的杀虫活性，其中至少一种(一种或两种)所述TC蛋白质增效剂相对于它发挥增强作用的TC蛋白质而言是异源TC蛋白质。毒素和用于增强该毒素的增效剂的组包括以下结合
很明显，上面的矩阵试图包括例如与诸如XptA1和/或XptA2(以及TcbA和/或TcdA)的任意毒素组合的TcdB2+TccC3(优选的增效剂对)。
对于利用本公开的本领域技术人员其它实施方案和组合将是明显的。
本发明还提供“混合对”增效剂，例如上面图表说明的增效剂对3、4和5。在此之前没有预期(或暗示)此类组合具有TC蛋白质毒素增强剂的活性。因此，目前增效剂的此类“异源”组合能够选择用于使其增强(例如)两种杀虫毒素的能力最大化。也就是说，对于特定的使用，目前人们可以发现例如TcdB1和XptB1是比XptC1和XptB1更加令人期望的增效剂对。此外，令人惊奇地给出了所给定的光杆状菌属增效剂和其可以替代的致病杆菌属增效剂之间序列趋异性的相对程度，以及增效剂天然增强的天然“靶”毒素之间差异的程度。所以，很显然本发明还提供异源增效剂对(即，B类(～170kDa)增效剂来自于与C类(～112kDa)增效剂所来源的细菌属不同的细菌属)。
本发明不限于280kDa TC蛋白质毒素和异源112kDa和/或170kDaTC蛋白质增效剂。由于这是首次发现“混合和匹配”致病杆菌属和光杆状菌属例如TC蛋白质的能力，本发明包括致病杆菌属TC蛋白质用“相应”光杆状菌属TC蛋白质的任意替代，并且反之亦然。例如，本领域技术人员目前也将寻找使用涉及“毒素C”成分(如上面背景部分中所论述)和“毒素D”成分的多种异源组合(例如，TccA+XptD1)。
例如，本发明还包括产生与一种或多种苏云金芽孢杆菌Cry蛋白质结合的受试TC蛋白质的转基因植物的用途。
蛋白质和毒素本发明提供易于施用的功能性的蛋白质。本发明还提供递送具有功能活性并且对多数目的昆虫，优选地是鳞翅类昆虫有效的昆虫杀虫剂的方法。“功能活性”(或者“针对的活性”)在此处意思是指蛋白质毒素作为口服活性的控制昆虫的试剂发挥作用(单独或者与其它蛋白质相结合)，从而所述蛋白质具有毒性效应(单独或者与其它蛋白质相结合)或者能够破坏或阻止昆虫生长和/或取食但其可能导致或不导致昆虫的死亡。当昆虫与通过转基因植物表达、制剂的蛋白质组合物、可喷射的蛋白质组合物、诱饵基质或其它递送系统递送的有效量的本发明“毒素”接触时，结果一般是昆虫死亡、昆虫生长和/或增殖的抑制、和/或阻止昆虫从食物来源(优选地是转基因植物)摄食，其中所述食物来源为昆虫从该食物来源中可以得到毒素。本发明的功能蛋白质还可以一起或单独起作用以增强或者促进一种或一种以上其它毒素蛋白质的活性。如文中所使用，术语“有毒的”、“毒性”或者“毒素”意思是表达本发明的“毒素”是具有文中所定义的“功能活性”
虽然对于喂食的昆虫完全致死是优选的，但是完全致死不需要达到功能活性。如果昆虫避免毒素或者停止摄食，既使在作用是亚致死的或者死亡推迟了或是间接的情况下，在一些应用中这种避免会是有用的。例如，如果想得到抗昆虫的转基因植物，昆虫不愿摄食该植物同对昆虫的致死毒性同样有用，因为最终目标是避免昆虫所导致的植物的损害。
有许多其它方法可以将毒素掺入到昆虫的食物中。例如通过对食物喷射如此处公开的蛋白质溶液将毒性蛋白质掺入到幼虫食物源中是可能的。备选地，可以将纯化的蛋白质经遗传工程改造进入无害的细菌中，然后将该细菌在培养基中进行生长并且应用到食物源中或者允许存在于想要根除昆虫的区域内的土壤中。还可以将蛋白质经过遗传工程改造直接进入昆虫的食物源。例如，对于许多昆虫幼虫的主要食物源是植物材料。因此，可以将编码毒素的基因转移到植物材料中以致于此植物材料能够编码目的毒素。
将功能活性转移到植物或者微生物系统通常需要将编码毒素的氨基酸序列的核苷酸序列整合进入蛋白质表达载体上，该蛋白质表达载体对于载体所要居留的宿主细胞是恰当的。得到编码具有功能活性蛋白质的核苷酸序列的一种方式是使用从毒素的氨基酸序列(如文中所公开)推导出来的信息从产生毒素的细菌物种中分离天然的遗传物质。可以对天然的序列进行优化以便在植物中表达，例如，如下面更详细的讨论。优化的多核苷酸也可以根据蛋白质序列进行设计。
本发明提供了具有毒素活性的多类TC蛋白质。表述这些毒素类和编码这些毒素的多核苷酸的一种方式是通过在具体条件的范围内，其与例证的核苷酸序列(其互补序列和/或来源于任一条链的探针)杂交的能力和/或通过使用来源于例证序列的引物进行PCR而扩增它们的能力来定义多核苷酸。
根据本发明可以用多种方法得到能够使用的杀虫毒素。例如，此处公开的杀虫毒素的抗体可以用来从蛋白质混合物中鉴定和分离其它的毒素。具体而言，针对毒素中最恒定和最能与其它毒素相区别的部分来产生抗体。通过免疫沉淀、酶联免疫吸附测定(ELISA)或者免疫印迹可以使用这些抗体特异地鉴定具有特征活性的等效毒素。使用标准的操作步骤可以容易地制备针对此处所公开的毒素、或者等效毒素、或者这些毒素片段的抗体。此类抗体是本发明的一个方面。本发明的毒素可以从大量来源的微生物中得到。
本领域技术人员可以清楚地认识到本发明的毒素(和基因)可以从多种来源中得到。毒素“从”或者“自……获得”任何分离株在此处指的意思或建议的意思是毒素(或者相似的毒素)可以从分离株或者诸如另一细菌菌株或者植物的其它一些来源得到。“来自……”也具有该含义并且包括从例如经过修饰用于在植物中进行表达的特定类型的自细菌中得到的蛋白质。本领域技术人员会清楚地认识到，一旦细菌基因和毒素被公开，可以将植物进行工程改造以产生该毒素。使用此处公开的多核苷酸和/或氨基酸序列可以制备抗体、核酸探针(DNA和RNA)等等，并且这些抗体、核酸探针等等可以用来从其它(天然)来源中筛选和回收其它毒素基因。
多核苷酸和探针本发明进一步提供了编码根据本发明使用的TC蛋白质的核苷酸序列。本发明进一步提供了鉴定和表征编码具有毒素活性蛋白质的基因。在一个实施方案中，本发明提供了独特的核苷酸序列，该序列用作杂交探针和/或用于PCR技术的引物。这些引物可以产生能够用于鉴定、表征、和/或分离特异毒素基因的特征性基因片段。本发明的核苷酸序列编码与先前叙述的毒素不同的毒素。
本发明的多核苷酸可以用来形成在目的宿主细胞中编码蛋白质或者肽的完整“基因”。例如，如本领域技术人员能够清楚地认识到，本多核苷酸可以合适地置于目的宿主启动子的控制之下，这在本领域是已知的。
如本领域技术人员所知，DNA一般以双链形式存在。在这种排列中，一条链与另外一个链互补并且反之亦然。由于(例如)在植物中DNA是可以复制的，所以DNA的额外互补链可以产生。在本领域中经常使用“编码链”来指能够与反义链接合的链。mRNA从DNA的“反义”链上进行转录。在“有义”或者“编码”链上具有一系列的密码子(密码子是可以作为三个残基单位进行阅读以指定一个具体氨基酸的三核苷酸)，其可以作为开放阅读框(ORF)进行阅读以形成目的蛋白质或者肽。为了在体内产生蛋白质，DNA的一条链一般转录成能够作为蛋白质模板使用的mRNA的互补链。因此，本发明包括在所附的序列表中所示的例证性多核苷酸和/或包括互补链的等效物的用途。与例证DNA功能等同的RNA和PNA(肽核酸)也包括在本发明之内。
在本发明的一个实施方案中，在导致微生物高度增殖的条件下培养细菌分离株。在处理微生物以提供单链基因组核酸后，将DNA与本发明的引物接触并且进行PCR扩增。通过所述操作步骤可以对编码毒素的基因的特征片段进行扩增，从而可以鉴定编码毒素的基因的存在。
本发明的一个进一步的方面包括使用文中公开的方法和核苷酸序列鉴定的基因和分离株。由此鉴定的基因编码抗害虫的毒素活性。
通过使用例如寡核苷酸探针可以鉴定和得到本发明有用的蛋白质和基因。探针是能够依靠恰当标记可以检测的可检测核苷酸序列或者如国际申请序列号WO 93/16094中所述的制作成固有的荧光。探针(和本发明的多核苷酸)可以是DNA、RNA或者PNA。除了腺嘌呤(A)，胞嘧啶(C)，鸟嘌呤(G)，胸腺嘧啶(T)和尿嘧啶(U；对于RNA分子)外，本发明的合成探针(和多核苷酸)还可以含有次黄嘌呤(能够与所有四种碱基配对的中性碱基；有时用于代替合成的探针中所有四种碱基的混合物)。因此，当文中谈及合成的简并寡核苷酸，并且通常使用“N”或者“n”时，“N”或者“n”可以是G，A，T，C或者次黄嘌呤。文中所使用多义密码子在本申请文件中与标准的IUPAC命名规则相一致(例如，R指A或者G，Y指C或者T等)。
如本领域所熟知，如果探针分子与核酸样品杂交，可以有理由认为探针和样品具有相当的同源性/相似性/同一性。优选地，通过本领域众所周知的技术，如在Keller，G.H.，M.M.Manak(1987)DNA Probes，StocktonPress，New York，NY，169-170页中所述，多核苷酸首先进行杂交，然后在低、中或者高严格条件下进行洗涤。例如，如此处所述，通过在室温下用2×SSC(标准柠檬酸盐)/0.1％SDS(十二烷基硫酸钠)第一次洗涤15分钟实现低严格条件。一般进行两次洗涤。因此通过降低盐浓度和/或升高温度可以实现较高的严格条件。例如，在上述的洗涤后接着是用0.1×SSC/0.1％SDS在室温下洗涤15分钟的两次洗涤，再其次是用0.1×SSC/0.1％SDS在55℃条件下每次洗涤30分钟的多次洗涤。这些温度可以用于文中提出的其它杂交和洗涤操作步骤并且是本领域技术人员已知的(例如SSPE可以作为盐来代替SSC)。通过将50ml 20×SSC和5ml 10％SDS加入到445ml水中来配制2×SSC/0.1％SDS。通过组合NaCl(175.3g/0.150M)、柠檬酸钠(88.2g/0.015M)和水，用10N NaOH调节pH至7.0，然后调节体积至1升来配制20×SSC。通过在50ml灭菌水中溶解10g SDS，然后稀释到100ml来配制10％SDS。
探针检测提供了一种以已知的方式确定杂交是否维持的方法。此种探针分析提供了一种鉴定本发明编码毒素的基因的快速方法。作为本发明探针使用的核苷酸片段可以使用DNA合成仪和标准方法进行合成。这些核苷酸序列还可以用作PCR引物用于扩增本发明的基因。
分子的杂交特征可以用来定义本发明的多核苷酸。因此，本发明包括能够与文中例证的多核苷酸杂交的多核苷酸(和/或它们的互补链，优选地是它们的全长互补链)。即，例如，定义tcdA-样基因(和它编码的蛋白质)的一种方式为通过其与先前已知的(包括具体例证的)tcdA基因杂交(在此处公开的任何具体条件下)的能力而定义。例如对于xptA2-，tcaC-，tcaA-，tcaB-，tcdB-，tccC-和XptB1-样基因和相关蛋白质也是同样。这还包括tcdB2和tccC3基因。
如文中所使用，杂交的“严格”条件是指实现同当前申请所采用的条件相同或者大约相同程度的杂交特异性的条件。具体而言，通过标准的方法(见，例如Maniatis，T.，E.F.Fritsch，J.SambrookMolecularCloningA Laboratory Manual，Cold Spring Harbor Laboratory，ColdSpring Harbor，NY)使用32P-标记的基因特异的探针开展固相DNA杂交的Southern印迹。通常，杂交和随后的洗涤在允许检测靶序列的条件下进行。对于双链DNA基因探针，在6×SSPE，5×Denhardt′s溶液，0.1％SDS，0.1mg/ml变性DNA中，在低于DNA杂交体解链温度(Tm)20-25℃条件下过夜杂交。在下面的公式中叙述了解链温度(Beltz，G.A.，K.A.Jacobs，T.H.Eickbush，P.T.Cherbas和F.C.KafatosMethods ofEnzyymology，R.Wu，L.Grossman和K.Moldave[著者]Academic Press，New York 100266-285)Tm＝81.5℃-16.6Log[Na+]+0.41(％G+C)-0.61(％甲酰胺)-600/碱基对中双链体的长度。
洗涤通常如下进行(1)1×SSPE，0.1％SDS室温15分钟(低严格洗涤)，两次。
(2)0.2×SSPE，0.1％SDS，在Tm-20℃下洗涤15分钟(中等严格洗涤)，一次。
对于寡核苷酸探针，在6×SSPE，5×Denhardt′s溶液，0.1％SDS，0.1mg/ml变性DNA中，在低于杂交体解链温度(Tm)10-20℃条件下过夜杂交。对于寡核苷酸探针的Tm可以通过下式确定Tm(℃)＝2(T/A碱基对数目)+4(G/C碱基对数目)(Suggs，S.V.，T.Miyake，E.H.Kawashime，M.J.Johnson，K.Itakura和R.B.WallaceICN-UCLA Symp.Dev.Biol.Using Purified Genes，D.D.Brown[ed.]，Academic Press，New York，23683-693)。
洗涤通常如下进行(1)1×SSPE，0.1％SDS室温15分钟(低严格洗涤)，两次。
(2)1×SSPE，0.1％SDS，在杂交温度下洗涤15分钟(中等严格洗涤)，一次。
通常，改变盐和/或温度以改变严格度。对于标记的大约＞70碱基长度的DNA片段，可以使用下面的条件低1或者2×SSPE，室温低1或者2×SSPE，42℃中0.2×或者1×SSPE，65℃
高0.1×SSPE，65℃。
双链体的形成和稳定性取决于杂合体两条链之间的基本互补性，并且，如上指出，能够耐受一定程度的错配。因此，本发明的探针序列包括所述序列的突变(单个和多重突变)、缺失、插入，及其组合，其中所述突变、插入和缺失允许与目的靶多核苷酸形成稳定杂交体。突变、插入和缺失能够以许多方式在给定的多核苷酸序列内产生，并且这些方法是本领域普通技术人员熟知的。其它方法将来可以逐渐为人们所知。
PCR技术聚合酶链式反应(PCR)是重复的、酶促的、使用引物的核酸序列合成。该过程是众所周知的并且本领域技术人员常常使用(见Mullis，美国专利号4,683,195、4,683,202和4,800,159；Saiki，Randall K.，StephenScharf,Fred Faloona，Kary B.Mullis，Glenn T.Horn，Henry A.Erlich，Norman Amheim“β-球蛋白基因组序列的酶促扩增和限制性酶切位点分析用于诊断镰刀形红细胞贫血症”，Science 2301350-1354)。PCR是基于目的DNA序列的酶促扩增，目的序列的两侧为可与靶序列互补链杂交的两条寡核苷酸引物。引物用指向彼此的3′末端表示方向。模板热变性、引物退火至其互补序列和使用DNA聚合酶延伸退火的引物步骤的重复循环导致由PCR引物5′末端所限定的片段的扩增。每条引物的延伸产物能够作为模板用于其它引物，因此每个循环基本上加倍以前循环中所产生的DNA片段的数量。这导致特异性靶片段的指数积累，在几个小时内增加至几百万倍。通过使用诸如Taq聚合酶(从嗜热菌嗜热水生菌(Thermusaquaticus)分离)的热稳定DNA聚合酶扩增过程能够完全自动化。其它能够使用的酶是本领域技术人员熟知的。
本发明的DNA序列能够用作PCR扩增的引物。进行PCR扩增的过程中，能够耐受引物与模板之间一定程度的错配。因此，作为例证的引物的突变、缺失和插入(特别是将核苷酸加至5′末端)包括在本发明的范围之内。通过普通技术人员已知的方法能够在给定引物中产生突变、插入和缺失。
基因和毒素的修饰根据本发明有用的基因和毒素不仅包括特定的作为例证的全长序列，还包括这些序列、变体、突变体、嵌合体的部分、节段和/或片段(包括与全长分子相比的内部和/或末端缺失)，及其融合物。本发明的蛋白质可具有替换的氨基酸，只要它们保留此处特定作为例证的蛋白质的特异杀虫/功能活性。“变体”基因具有编码相同毒素或具有等效于作为例证蛋白质的杀虫活性的等效毒素的核苷酸序列。术语“变体蛋白质”和“等效毒素”是指具有与作为例证的毒素相同或基本上相同的针对靶害虫的生物学/功能活性和等效序列的毒素。如文中所使用，当谈及“等效”序列是指具有能够提高杀虫活性或对杀虫活性无不利影响的氨基酸替换、缺失、添加或插入的序列。该定义中还包括了保留杀虫活性的片段。保留与作为例证的毒素的相应片段相同或相似功能或“毒素活性”的片段和其它等效物也包括于本发明的范围之内。变化，例如氨基酸置换或添加，能够用于多种目的，例如增加(或降低)蛋白质的蛋白酶稳定性(而没有大大地/相当大地降低毒素的功能活性)。
等效毒素和/或编码这些等效毒素的基因能够获得于/来自于野生型或重组细菌和/或使用此处所提供的技术获得于/来自于其它野生型或重组生物体。例如，其它芽胞杆菌属、沙雷氏菌属、类芽胞杆菌属、光杆状菌属和致病杆菌属能够用作来源分离株。
使用例如用于点突变的标准技术可以容易地构建基因的变化。此外，例如，美国专利号5,605,793叙述了通过在随机片段化后利用DNA重排产生额外的分子多样性的方法。变体基因能够用于产生变体蛋白质；重组宿主能够用于产生变体蛋白质。利用这些“基因改组(gene shuffling)”技术，能够构建包含此处作为例证的任意序列的任意5、10或20个连续残基(氨基酸或核苷酸)的等同基因和蛋白质。如本领域技术人员所知，基因步移技术能够进行调整以获得具有例如3，4，5，6，7，8，9，10，11，12，13，14，15，16，17，18，19，20，21，22，23，24，25，26，27，28，29，30，31，32，33，34，35，36，37，38，39，40，41，42，43，44，45，46，47，48，49，50，51，52，53，54，55，56，57，58，59，60，61，62，63，64，65，66，67，68，69，70，71，72，73，74，75，76，77，78，79，80，81，82，83，84，85，86，87，88，89，90，91，92，93，94，95，96，97，98，99，100，101，102，103，104，105，106，107，108，109，110，111，112，113，114，115，116，117，118，119，120，121，122，123，124，125，126，127，128，129，130.131，132，133，134，135，136，137，138，139，140，141，142，143，144，145，146，147，148，149，150，151，152，153，154，155，156，157，158，159，160，161，162，163，164，165，166，167，168，169，170，171，172，173，174，175，176，177，178，179，180，181，182，183，184，185，186，187，188，189，190，191，192，193，194，195，196，197，198，199，200，201，202，203，204，205，206，207，208，209，210，211，212，213，214，215，216，217，218，219，220，221，222，223，224，225，226.227，228，229，230，231，232，233，234，235，236，237，238，239，240，241，242，243，244，245，246，247，248，249，250，251，252，253，254，255，256，257，258，259，260，261，262，263，264，265，266，267，268，269，270，271，272，273，274，275，276，277，278，279，280，281，282，283，284，285，286，287，288，289，290，291，292，293，294，295，296，297，298，299，300，301，302，303，304，305，306，307，308，309，310，311，312，313，314，315，316，317，318，319，320，321，322，323，324，325，326，327，328，329，330，331，332，333，334，335，336，337，338，339，340，341，342，343，344，345，346，347，348，349，350，351，352，353，354，355，356，357，358，359，360，361，362，363，364，365，366，367，368，369，370，371，372，373，374，375，376，377，378，379，380，381，382，383，384，385，386，387，388，389，390，391，392，393，394，395，396，397，398，399，400，401，402，403，404，405，406，407，408，409，410，411，412，413，414，415，416，417，418，419，420，421，422，423，424，425，426，427，428，429，430，431，432，433，434，435，436，437，438，439，440，441，442，443，444，445，446，447，448，449，450，451，452，453，454，455，456，457，458，459，460，461，462，463，464，465，466，467，468，469，470，471，472，473，474，475，476，477，478，479，480，481，482，483，484，485，486，487，488，489，490，491，492，493，494，495，496，497，498，499或500个连续残基(氨基酸或核苷酸)、对应任意作为例证或所建议的序列(或其互补序列(完全互补序列))中的片段(相同大小)的等同物。相似大小的片段，特别是那些保守区相似大小的片段，能够用作探针和/或引物。
利用商业可得的核酸外切酶或核酸内切酶，按照标准步骤能够制备全长基因的片段。例如，诸如Bal31的酶或定点诱变能够用于从这些基因的末端系统地切除核苷酸。同样，利用多种限制性内切酶可以获得编码活性片段的基因。蛋白酶可以用于直接获得这些毒素的活性片段。
如此处所述，毒素(和TC蛋白质)可以被截短而仍然保留功能活性也在本发明的范围之内。“截短的蛋白质”意思是指一部分毒素蛋白质可以被切割并且切割之后仍然呈现活性。通过昆虫肠道内部或外部的蛋白酶能够实现切割。而且，使用标准分子生物学技术能够产生有效切割的蛋白质，其中编码所述毒素的DNA碱基通过用限制性内切酶消化或者使用技术人员可用的其它技术移出。截短之后，所述蛋白质能够在异源系统例如大肠杆菌、杆状病毒、基于植物的病毒系统、酵母等等进行表达，并且然后置于此处所公开的昆虫测定法中确定其活性。在本领域中众所周知，能够成功地产生截短的毒素，以至于虽然小于完整的全长序列，它们仍保留了功能活性。在本领域中众所周知，B.t.毒素能够以截短的(核心毒素)形式使用。见，例如，Adang等人，Gene 36289-300(1985)，“苏云金芽孢杆菌亚种kurstaki HD-73的晶体蛋白质的全长和截短的质粒克隆特性表征及其对烟草天蛾的毒性”。保留杀虫活性的截短蛋白质的其它例子包括昆虫保幼激素酯酶(Regents of University of California的美国专利号5,674,485)。如文中所使用，术语“毒素”意思也包括功能上有活性的截短形式。
在一些情况下，尤其对于在植物中表达，使用表达截短蛋白质的截短基因是有利的。例如，在上面背景部分所论述，Hofte等人，1989，论述了B.t.毒素的前毒素和核心毒素片段。优选的截短基因一般编码全长蛋白质的40，41，42，43，44，45，46，47，48，49，50，51，52，53，54，55，56，57，58，59，60，61，62，63，64，65，66，67，68，69，70，71，72，73，74，75，76，77，78，79，80，81，82，83，84，85，86，87，88，89，90，91，92，93，94，95，96，97，98或99％。例如背景部分还论述了加工和重排TcdA和TcbA片段的蛋白酶。
本发明的某些毒素/TC蛋白质已经在文中明确地举例说明。由于这些毒素/TC蛋白质仅仅是本发明蛋白质的例证，非常明显本发明包含与例证蛋白质具有相同或相似毒素活性的变体或等效蛋白质(和编码其等效物的核苷酸序列)。等效蛋白质与作为例证的毒素/TC蛋白质具有氨基酸相似性(和/或同源性)。氨基酸同一性一般将大于60％，优选地大于75％，更加优选地大于80％，甚至更加优选地大于90％，并且可大于95％。本发明优选的多核苷酸和蛋白质也可根据更加特定的同一性和/或相似性范围定义。例如，与此处作为例证或建议的序列相比，同一性和/或相似性可以是49，50，51，52，53，54，55，56，57，58，59，60，61，62，63，64，65，66，67，68，69，70，71，72，73，74，75，76，77，78，79，80，81，82，83，84，85，86，87，88，89，90，91，92，93，94，95，96，97，98或99％。上面列出的任意数字能够用于定义上限和下限。例如，A类蛋白质能够定义为与给定的TcdA蛋白质具有50-90％的同一性。因此，与任意先前已知的TcdA蛋白质(包括此处特别举例说明的任意TcdA蛋白质(并且同样地XptA2蛋白质))相比，通过此处所提供或建议的任意以数字表示的同一性分值能够定义TcdA-样蛋白质(和/或tcdA-样基因)。根据本发明，这同样适用于任意蛋白质或基因，例如TcaC-、TcaA-、TcaB-、TcdB-、TccC-和XptB2-样蛋白质和基因。因此，这适用于增效剂(例如TcdB2和TccC3)和独特毒素。
除非另外声明，使用Karlin和Altschul(1990)，Proc.AM.Acad.Sci.USA 872264-2268的算法，该算法如在Karlin和Altschul(1993)，Proc.Natl.Acad.Sci.USA 905873-5877中进行了改进，确定如文中所使用的两条核酸的百分比序列同一性和/或相似性。此种算法被整合入Altschul等人(1990)，J.Mol.Biol.215402-410的NBLAST和XBLAST程序。使用NBLAST程序进行BLAST核苷酸检索，分值＝100，字长＝12。可如Altschul等人(1997)，Nucl.Acids Res.253389-3402中所述使用缺口BLAST。当利用BLAST和缺口BLAST程序时，使用各程序(NBLAST和XBLAST)的缺省参数。见NCBI/NIH网站。如上面背景部分所述，利用Crickmore等人的方法和算法也能够计算分值。为了获得用于比较目的的缺口比对，使用了Vector NTI Suite 8(InforMax，Inc.，North Bethesda，MD，U.S.A.)的Align X函数，其中采用了缺省参数。这些是缺口开口罚分15，缺口延伸罚分6.66并且缺口分离罚分8。
在蛋白质的关键区域氨基酸同源性/相似性/同一性是最高的，这些关键区域负责着毒素的活性或者参与确定最终负责毒素活性的三维构象。在这点上，某些氨基酸替代是可接受的并且预计是可以忍受的。例如，这些替代可以是在蛋白质非关键活性的区域。可以使用蛋白质晶体结构分析和基于软件的蛋白质结构建模来确定蛋白质中能够被修饰的区域(使用定点诱变、改组等)，以确实改变蛋白质的特性和/或提高蛋白质的功能性。
还可以对蛋白质的多种特性和三维特征进行改变而对蛋白质的毒素活性/功能性无不利影响。保守氨基酸的替代预计能够忍受/不会不利地影响分子的三维构象。氨基酸可以分为下面的几类非极性的、不带电荷的极性的、碱性的和酸性的。一类中的氨基酸被该类中的另一个氨基酸替代的这种保守替代处于本发明的范畴，条件是只要替代不为不利于所述化合物生物学活性的替代即可。
表1 提供了属于每一类的氨基酸的例子的列表
在一些情况下，也可以进行非保守替代。关键的因素是这种替代必须不明显损失蛋白质的功能/生物学/毒素活性。
如文中所使用，当谈及“分离的”多核苷酸和/或“纯化的”毒素是指没有与天然存在的其它分子相关联的这些分子。因此，如文中所使用，当谈及“分离的”和/或“纯化的”意思是存在“人工”的参与。例如，置入植物中用来表达的本发明细菌毒素“基因”是“分离的多核苷酸”。同样，此处例证的由植物产生的类芽孢杆菌属蛋白质是“分离的蛋白质”。
由于遗传密码的简并性/冗余性，多种不同的DNA序列可以编码此处公开的氨基酸序列。产生编码相同或者基本上相同毒素的备选DNA序列是受过本领域培训的技术人员的技术范围之内。这些变体DNA序列属于本发明的范畴。
优化序列用于在植物中表达为了实现异源基因在植物中高表达，优选地是对所述基因重新进行改造使得它们能够在植物细胞的(胞质)中更有效的表达。玉米是此种植物的一种，优选地是在转化前将外源基因进行重新设计以提高它们在所述植物中的表达水平。因此，设计编码细菌毒素的基因的额外步骤是改造异源基因用于优化表达。
对重新改造细菌毒素用于在玉米中表达的一个原因是天然基因的G+C含量是非优化的。例如，许多天然的细菌基因G+C含量非常低(并且结果向高A+T含量倾斜)导致了模拟或者重复已知高度富含A+T的植物基因控制序列的产生。在导入植物的基因DNA中一些富含A+T的序列的存在(例如正常情况下在基因启动子中发现的TATA盒区域)会导致异常的基因转录。另一方面，位于转录的mRNA(例如多聚腺苷酸化信号序列(AAUAAA)或者与参与前体-mRNA剪接的小核RNA互补的序列)中的其它调节序列的存在可能导致RNA不稳定。因此，在对用于在玉米中表达的编码细菌毒素基因(更加优选地称为为植物优化的基因)的设计中的一个目标是产生具有较高G+C含量的DNA序列，并且优选地是与编码代谢酶玉米的基因相近的G+C含量。在设计编码细菌毒素的植物优化的基因的另一个目标是产生这样的DNA序列，其中序列的修饰不阻碍翻译。
下面的表(表2)表明在玉米中G+C％含量是如何高。对于表2中的数据，基因的编码区取自GenBank(版本71)条目，并且使用MacVectorTM程序(Accelerys，San Diego，California)计算了碱基组成。在计算中忽略了内含子序列。
由于遗传密码的冗余性/简并性所赋予的可塑性(即一些氨基酸被多于一个的密码子所指定)，不同生物或者不同生物类别的基因组的进化导致对冗余密码子的不同使用。这种“密码子偏倚性”反映在蛋白质编码区的平均碱基组成上。例如，具有相对低G+C含量的生物利用冗余密码子中第三位为A或者T的密码子，而具有较高G+C含量的生物利用第三位为G或者C的密码子。据认为在mRNA中“稀有”密码子的存在降低了mRNA的绝对翻译速率，特别是当对应于稀有密码子的氨酰tRNA的相对丰度很低时。将此扩展，由于单个稀有碱基导致的翻译速率的降低对于多个稀有碱基而言至少为加成的。因此，具有相对高的稀有密码子含量的mRNA相应地具有低的翻译速率。该速率会反映在后来所编码蛋白质的低水平上。
在用于在玉米(或其它植物，例如棉花或者大豆)中表达的编码细菌毒素的工程改造基因中，确定了植物的密码子偏倚性。对于玉米的密码子偏倚性是该植物用来编码其蛋白质的统计上的密码子分布，并且优选的密码子选择示于表3。在确定了偏倚性之后，确定目的基因中密码子的频率百分数。应该确定植物优选的主要密码子，当存在多种选择时还应该确定优选密码子的第二、第三和第四种选择。因此可以设计出编码细菌毒素的氨基酸序列的新DNA序列，但是通过在毒素氨基酸序列的每一个位置上的氨基酸用植物(第一优选、第二优选、第三优选或者第四优选)密码子进行替代使得新的DNA序列不同于天然的细菌DNA序列(编码毒素)。然后对新的序列进行限制性酶切位点的分析，这些酶切位点可能通过修饰而产生。将鉴定出来的位点通过用第一、第二、第三或者第四选择的优选密码子进行替代来进一步进行修饰。可能影响目的基因转录和翻译的序列中的其它位点是外显子内含子连接处(5′或者3′)、多聚A添加信号或者RNA多聚酶终止信号。将序列进行进一步的分析和修饰以降低TA或者GC双联体的频率。除了双联体外，具有多于约4个同一残基的G或者C的序列区会影响序列的转录。因此，通过用次优选的所选密码子替代第一或者第二选择的密码子等等也可以对所述区域进行修饰。
a在括弧内给出该类中的基因数目。
b在括弧内给出标准差。
c在平均值计算中忽略了组合组平均值。
在编码细菌毒素的植物优化的基因中含有大约63％第一选择密码子、大约22％到37％之间的第二选择密码子和大约15％到大约0％之间的第三或者第四选择密码子是优选的，其中总的百分数是100％。植物优化的基因中含有大约63％第一选择密码子、至少大约22％第二选择密码子、大约7.5％第三密码子和大约7.5％第四密码子是最优选的，其中总的百分数是100％。用于在玉米中表达的工程改造基因中的优选密码子使用示于表3。上述方法能够使本领域技术人员修饰对特定植物而言为外来的基因，从而该基因在植物中优化表达。在PCT申请WO 97/13402中做了进一步阐述。
为了设计编码细菌毒素的植物优化基因，使用如表2所示的，从针对具体植物的基因序列编辑的密码子偏倚性表中建立的丰余遗传密码，将DNA序列设计成能够编码所述蛋白质毒素的氨基酸序列。所获得的DNA序列具有较高程度的密码子多样性，所期望的碱基组成可以含有战略上设置的限制性酶识别位点，并缺乏可能干扰基因转录或者产物mRNA翻译的序列。
*用于玉米的第一和第二优选密码子因此，功能等效于本发明毒素基因的人工基因可以用来转化包括植物在内的宿主。关于产生人工基因的额外的指导可以在例如美国专利号5,380,831中找到。
转基因宿主可以将本发明的编码毒素的基因导入广泛的多种微生物或者植物宿主中。在优选的实施方案中，使用了转基因植物细胞和植物。优选的植物(和植物细胞)是谷物、玉米和棉花。
在优选的实施方案中，毒素基因的表达直接或者间接导致杀虫蛋白质在细胞内产生(和维持)。以这种方式植物表现出昆虫抗性。当害虫摄取转基因/重组/转化/转染的宿主细胞(或其内容物)时，害虫会摄取毒素。这是导致害虫和毒素接触的优选方式。结果是对害虫的控制(杀死或者致病)。对于吮吸害虫也可以用相似的方式进行控制。备选地，在靶害虫存在的地方可以使用适宜的微生物宿主，例如假单胞菌属如荧光假单胞菌(P.fluorescens)，微生物可以在此处增殖并且被靶害虫摄取。可以将具有毒素基因的微生物在延长毒素活性和稳定细胞的条件下进行处理。从而经过处理的保留毒素活性的细胞可以应用到靶害虫的环境中。
如果通过适当的载体将毒素基因导入微生物宿主，并且将所述宿主以活的状态应用到环境中时，可以用到一些宿主微生物。选择了已知占具有一种或多种目的作物植物圈(叶面、叶圈、根际和/或根面)的微生物宿主。将这些微生物进行选择以致于具有在具体环境中(作物和其它昆虫栖息地)成功竞争野生型微生物的能力，提供能够表达多肽杀虫剂的基因的稳定维持和表达，并且，希望提供使杀虫剂免受环境降解和失活的增强的保护。
已知大量的微生物栖息在广泛多样的重要作物的叶面(植物叶的表面)和/或根际(植物根周围的土壤)。这些微生物包括细菌、藻类和真菌。特别感兴趣的是微生物，诸如细菌，例如假单胞菌属、欧文氏菌属(Erwinia)、沙雷氏菌属、克雷伯氏菌属(Klebsiella)、黄单胞菌属(Xanthomonas)、链霉菌属(Streptomyces)、根瘤菌属(Rhizobium)、红假单胞菌属(Rhodopseudomonas)、Methylophilius、农杆菌属(Agrobacterium)、醋杆菌属(Acetobacter)、乳杆菌属(Lactobacillus)、节杆菌属(Arthrobacter)、固氮菌属(Azotobacter)、明串珠菌属(Lezscorzostoc)和产碱菌属(Alcaligenes)；真菌，特别是酵母，例如酵母属(Saccharomyces)、隐球酵母属(Cryptococcus)、克鲁维酵母属(Kluyveromyces)、掷孢酵母属(Sporobolomyces)、红酵母属(Rhodotorula)和短梗霉属(Aureobasidium)。特别感兴趣的是植物圈细菌物种如丁香假单胞菌(Pseudomonas syringae)、荧光假单胞菌、粘质沙雷氏菌(Serratia marcescens)、木醋杆菌(Acetobacter xylinum)、根癌农杆菌(Agrobacterium tumefaciens)、球形红假单胞菌(Rhodopseudomonas spheroides)、野油菜黄单胞菌(Xanthomonascampestris)、苜蓿根瘤菌(Rhizobum melioti)、真养产碱菌(Alcaligenesentrophus)和维涅兰德固氮菌(Azotobacter vinlandii)；和植物圈酵母物种如深红酵母(Rhodotorula rubra)、红酵母(R.glutinis)、海滨红酵母(R.marina)、橙黄红酵母(R.aurantiaca)、浅白隐球酵母(Cryptococcusalbidus)、流散隐球酵母(C.diffluens)、变黄罗伦隐球酵母(C.laurentii)、罗斯酵母(Saccharomyces rosei)、普地酵母(S.pretoriensis)、酿酒酵母(S.cerevisiae)、掷孢酵母(Sporobomyces roseus)、香气掷孢酵母(S.odorus)、佛地克鲁维酵母(Kluyveromyces veronae)和出芽短梗霉(Aureobasidium pollulans)。也感兴趣的是有颜色的微生物。
将基因插入以形成转基因宿主本发明的一个方面是用表达本发明蛋白质的本发明多核苷酸转化/转染植物、植物细胞和其它宿主细胞。用此种方式转化的植物具有抗靶害虫攻击的抗性。
可以用广泛大量的方法在允许基因稳定维持和表达的条件下将编码杀虫蛋白质的基因导入靶宿主。这些方法对于本领域技术人员而言是熟知的并且在例如美国专利号5,135,867中有所叙述。
例如，包含大肠杆菌复制系统和允许对转化细胞进行选择的标记物的大量克隆载体可用于将外来基因插入到高等植物中。载体包含例如pBR322、pUC系列、M13mp系列、pACYC184等等。相应地，可以将编码毒素的序列插入到载体的适当限制性位点。所得到的质粒用于转化入大肠杆菌。将大肠杆菌在适当的营养培养基中进行培养，然后收获和裂解。将质粒回收。通常实施序列分析、限制性分析、电泳和其它生物化学-分子生物学方法作为分析的方法。每一次操作之后，将所使用的DNA序列进行切割并且连接到下一个DNA序列中。可以将每一个质粒序列克隆到相同的或者其它的质粒中。根据目的基因插入植物的方法，其它的DNA序列可能是需要的。例如，如果Ti或者Ri质粒用于转化植物细胞，那么至少是Ti或者Ri质粒T-DNA的右边界，但常常是右边界和左边界，作为所插入基因的侧翼区而连接。对于T-DNA用于转化植物细胞的用途已经得以广泛研究并在EP 120516；Hoekema(1985)The Binary Plant VectorSystem，Offset-durkkerii Kanters B.v.，Alblasserdam，第5章；Fraley等，Crit.Rev.Plant Sci.41-46；和An等(1985)EMBO J.4277-287中有叙述。
有大量的技术可以将DNA插入到植物宿主细胞。这些技术包括使用根癌农杆菌或者发根农杆菌(Agrobacterium rhizogenes)作为转化介质的T-DNA转化、融合、注射、生物弹射击法(biolistics)(微粒子轰击)或者电穿孔以及其它可能的方法。如果农杆菌属用于转化，必须将欲被插入的DNA克隆到特定的质粒中，即克隆到中间载体或者双元载体。由于序列与T-DNA上的序列同源，通过同源重组可以将中间载体整合到Ti或者Ri质粒中。Ti或者Ri质粒还含有对于T-DNA转移必需的vir区域。中间载体不能够在农杆菌属中自我复制。通过辅助质粒(接合)的方法可以将中间载体转移入根癌农杆菌。双元载体可以在大肠杆菌和农杆菌属中进行自我复制。它们包含选择性标记基因和接头或者多接头，这些接头或者多接头被T-DNA右边界和左边界框起来。它们可以直接转化进入农杆菌属中(Holsters等MoL Gen.Genet.163181-187)。作为宿主细胞使用的农杆菌属要包含携带vir区域的质粒。vir区域对于将T-DNA转移进入植物细胞是必需的。可以包含额外的T-DNA。如此转化的细菌用于转化植物细胞。植物外植体可以有利地与根癌农杆菌或者发根农杆菌一起培养，以便将DNA转移进入植物细胞。在含有用于选择的抗生素或者杀生物剂的适当培养基中使被感染的植物材料(例如叶片、茎段、根，还可以是原生质体或者悬浮培养的细胞)再生出整株植物。然后对如此得到的植物检验插入DNA是否存在。在注射和电穿孔的情况下，对质粒没有特殊的要求。使用普通的质粒，例如pUC衍生物是可行的。
通常转化的细胞生长在植物的内部。它们可以形成生殖细胞并且将转化的特性传给后代植物。此种植物可以以正常方式生长并可与转化有同样遗传因子或者其它遗传因子的植物进行杂交。所得的杂交个体具有相应的表型特征。
在本发明的一些优选实施方案中，编码细菌毒素的基因从插入植物基因组中的转录单元进行表达。优选地，所述转录单元是能够稳定整合进入植物基因组并能够对表达mRNA编码蛋白质的经转化植物品系进行筛选的重组载体。
一旦插入的DNA被整合进入基因组，则它在基因组中是相对稳定的(并且不能够再次出来)。正常情况下它含有赋予转化的植物细胞抗杀生物剂或者抗生素的选择性标记，诸如卡那霉素、G418、博来霉素、潮霉素或者氯霉素及其它。各种所应用的标记因此应该允许对转化细胞进行选择而不是对不含有插入DNA的细胞进行选择。目的基因优选地通过组成型或者诱导型启动子在植物细胞中表达。一旦表达，mRNA可以翻译成蛋白质，因此将目的氨基酸掺入到蛋白质中去。在植物细胞中表达的编码毒素的基因可以在组成型启动子、组织特异型启动子或者诱导型启动子的控制下。
存在一些用于将外来重组载体导入植物细胞并且得到稳定维持和表达所导入基因的植物的技术。此类技术包括将包被在微颗粒上的遗传物质直接导入细胞(Cornell的美国专利号4,945,050和DowElance的美国专利号5,141,131，现在为Dow AgroSciences，LLC)。此外，使用农杆菌属的技术将植物进行转化，见University of Toledo的美国专利号5,177,010；TexasA&M的美国专利号5,104,310；欧洲专利申请0131624B1；Schilperoot的欧洲专利申请120516，159418B1和176,112；Schilperoot的美国专利申请号5,149,645，5,469,976，5,464,763和4,940,838和4,693,976；MaxPlanck的欧洲专利申请116718，290799，320500；日本Tobacco的欧洲专利申请604662和627752和美国专利号5,591,616；Ciba Geigy的，现在为Novartis的欧洲专利申请0267159和0292435和美国专利号5,231,019；Calgene的美国专利号5,463,174和4,762,785和Agracetus的美国专利号5,004,863和5,159,135。其它的转化技术包括whiskers技术。见Zeneca的美国专利号5,302,523和5,464,765。电穿孔技术也用来转化植物。见BoyceThompson Institute的WO 87/06614；Dekalb的美国专利号5,472,869和5,384,253和Plant Genetic Systems的Wo 92/09696和WO 93/21335。此外，病毒载体也可以用于产生表达目的蛋白质的转基因植物。例如，使用Mycogen Plant Science和Ciba-Giegy现在为Novartis的美国专利号5,569,597以及Biosource的美国专利号5,589,367和5,316,931中叙述的方法可以用病毒载体转化单子叶植物。
如先前所提到，DNA构建体导入植物宿主的方式不是本发明的关键。能够提供有效转化的任何方法均可采用。例如，此处叙述了植物细胞转化的多种方法并且包括Ti或者Ri质粒等的使用进行农杆菌属介导的转化。在很多情况下，希望用于转化的构建体位于T-DNA边界的一侧或者两侧，更加优选的是位于右边界。虽然在其它转化模式中可见T-DNA边界的使用，但当此构建体用根癌农杆菌或者发根农杆菌作为转化模式时特别有用。当使用农杆菌属用于植物细胞转化时，欲被导入宿主的载体用来与存在于宿主中的T-DNA或者Ti或者Ri质粒进行同源重组。可以通过电穿孔、三亲杂交和本领域技术人员已知的用于转化革兰氏阴性细菌的其它技术实施载体的导入。载体转化进入农杆菌宿主的方式不是本发明的关键。包含用于重组的T-DNA的Ti或者Ri质粒能够或者不能够导致菌瘿的形成，并且这不是本发明的重点，只要所述此宿主中存在vir基因即可。
在用农杆菌属用于转化的一些情况下，位于T-DNA边界中的表达构建体插入到广谱的载体中，例如如在Ditta等(PNAS USA(1980)777347-7351)和EPO 0 120 515(此处引用作为参考)中所叙述的pRK2或其衍生物。表达构建体和T-DNA中包含如此处所述的一种或多种标记，所述标记允许对转化的农杆菌属和转化的植物细胞进行选择。采用的具体标记不是本发明的本质，优选的标记取决于所使用的宿主和构建体。
对于使用农杆菌转化植物细胞，将外植体和转化的农杆菌属结合并孵育足够的时间以便允许其转化。在转化后，通过用恰当的抗生素进行选择将农杆菌杀死，并且将植物细胞培养在恰当的选择培养基中。愈伤组织一旦形成，根据植物组织培养和植物再生领域内熟知的方法，通过采用适当的植物激素促进枝条的形成。然而，愈伤组织中间阶段并不总是必要。在枝条形成后，可以将此植物细胞转移至培养基中促进根的形成，从而完成植物的再生。然后将植物培养至结种并且所述种子可以用来建立未来的子代。不管转化的技术为何技术，编码细菌毒素的基因优选地掺入到基因转移载体上，通过在载体上包含植物启动子调节元件和诸如Nos等的3′非翻译转录终止区，使得该载体用来在植物细胞中表达此种基因。
除了用于转化植物的技术有多种外，与外来基因相接触的组织类型同样也会变化。此种组织包括但不限于胚发生组织、愈伤组织类型I、II和III、胚轴、分裂组织、根组织、在韧皮部表达的组织等等。使用此处叙述的恰当的技术，几乎所有植物组织在去分化过程中均能够被转化。
如上面所提到，如果期望的话，多种选择标记可以使用。对具体标记的优先使用根据本领域技术人员的判断，但是任何下面的选择标记可以与此处没有列出的但能够作为选择标记起作用的其它基因一起使用。此种选择标记包括但不限于编码对抗生素卡那霉素、新霉素和G418抗性的转座子Tn5的氨基糖苷磷酸转移酶(Aph II)和编码对草甘膦、潮霉素、氨甲喋呤、膦丝菌素(双丙氨膦)、咪唑啉酮、磺酰脲和诸如氯磺隆(chlorsulfuron)、溴草腈(bromoxynil)、茅草枯等的三唑嘧啶(triazolopyrimidine)除草剂抗性或者耐受的那些基因。
除了选择标记以外，还可以使用报告基因。在一些例子中，报告基因可以与或者不与选择标记一起使用。报告基因是这样的基因，它一般不存在于受体生物体或者组织中的并且一般编码导致一些表型改变或者酶特性的蛋白质。此种基因的例子在K.Wising等Ann.Rev.Genetics，22，421(1988)中有所提供。优选的报告基因包括大肠杆菌uidA基因座的β-葡糖醛酸糖苷酶(GUS)、来源于大肠杆菌Tn9的氯霉素乙酰转移酶基因、来源于发生物荧光的水母维多利亚水母(Aequorea victoria)的绿色荧光蛋白和来源于萤火虫北美萤火虫(Photiflus pyralis)的荧光素酶基因。在该基因被导入受体细胞适当时间后开展检测报告基因表达的分析。如Jefferson等(1987Biochem.Soc.Trans.15，17-19)所叙述，优选的此种方法是使用编码大肠杆菌uidA基因座的β-葡糖醛酸糖苷酶(GUS)的基因来鉴定转化的细胞。
除了植物启动子调节元件外，从多种来源的启动子调节元件可以在植物细胞中有效使用来表达外来基因。可以使用例如细菌来源的启动子调节元件，诸如章鱼氨酸合酶启动子、胭脂氨酸合酶启动子、marmopine合酶启动子，病毒来源的启动子，诸如花椰菜花叶病毒(35S和19S)、35T(它是一个重新进行工程改造的35S启动子，见美国专利号6,166,302，特别是实施例7E)等等。植物启动子调节元件包括但不限于核酮糖-1，6-二磷酸(RUBP)羧化酶小亚基(ssu)、β-伴大豆球蛋白(conglycinin)启动子、β-菜豆蛋白启动子、ADH启动子、热激启动子和组织特异启动子。还可以存在其它的元件诸如基质附着区、支架附着区、内含子、增强子、多聚腺苷酸化序列等等，并且这些元件可以促进转录效率或者DNA整合。此类元件对于DNA功能可以或不用是必需的，虽然它们可以通过影响转录、mRNA的稳定性等等来提供更好的DNA表达或者功能。此类元件可以如愿地包含在DNA中以便使已经转化的DNA在植物中有最佳的表现。一般的元件包括但不限于Adh-内含子1、Adh-内含子6、苜蓿花叶病毒衣壳蛋白前导序列、玉米条纹病毒衣壳蛋白前导序列以及本领域技术人员可得的其它元件。也可以使用组成型启动子调节元件从而指导基因在所有细胞类型和在所有的时间内持续表达(例如肌动蛋白、泛素、CaMV35S等)。组织特异型启动子调节元件负责基因在特异细胞或者组织类型诸如叶或者种子中表达(例如玉米醇溶蛋白、油质蛋白、napin、ACP、球蛋白等)，并且这些启动子也可以被使用。
启动子调节元件还可以是在植物发育的一定阶段活化的，也可以是在植物的组织和器官中活化的。此类启动子的例子包括但不限于花粉特异、胚特异、玉米穗丝特异、棉花纤维特异、根特异、种子胚乳特异的启动子调节元件等等。在一定情况下，使用可诱导启动子调节元件是所希望的，该元件负责对特异信号作出反应而使基因表达，其中所述特异的信号诸如物理刺激(热激基因)、光(RUBP羧化酶)、激素(Em)、代谢物、化学品和胁迫。可以使用在植物中有功能的其它目的转录和翻译元件。多种植物特异的基因转移载体在本领域是已知的。
使用标准的分子生物学技术对此处所述的毒素进行克隆和测序。其它信息可以在Sambrook，J.，Fritsch，E.F.，和Maniatis，T.(1989)，MolecularCloning，A Laboratory Manual，Cold Spring Harbor Press中找到，此处公开作为参考。
抗性管理随着杀虫蛋白质在植物中使用的日益商业化，抗性管理成为一种担心。即在其产品中使用苏云金芽孢杆菌毒素的公司有很多，并且对发展成抗B.t.毒素的昆虫存在忧虑。对昆虫抗性管理的一个策略是将由致病杆菌属、光杆状菌属等产生的TC毒素与诸如B.t.晶体毒素、来源于芽孢杆菌菌株的可溶性杀虫蛋白质(见，例如WO 98/18932和WO99/57282)或其它杀昆虫的毒素相结合。这种结合可以是配制成喷雾施用的制剂或者是分子的结合。可以用产生2种或者多种不同杀昆虫毒素的细菌基因转化植物(见，例如Gould，38Bioscience 26-33(1988)和美国专利号5,500,365；同样，欧洲专利申请0400246A1和美国专利5,866,784、5,908,970和6,172,281也叙述了用两种B.t.晶体毒素进行植物转化)。产生含有多于一种抗昆虫基因的转基因植物的另一种方法是首先产生2种植物，每种植物含有一种抗昆虫的基因。然后使用常规的植物育种技术将这些植物进行杂交以产生含有多于一种抗昆虫基因的植物。因此，很明显，如文中所使用，短语“包含多核苷酸”意思是指至少一种多核苷酸(并且可能更多，它们互相接近或者不接近)，除非另外特别指出。
制剂和其它递送系统所配制的含有本发明细胞和/或蛋白质(包括含有此处所述基因的重组微生物)的诱饵颗粒可以施用于土壤。所配制的产品还可以在作物循环的后期阶段作为种子包被、根处理或者全植物处理而施用。对植物和土壤进行处理的细胞可以采用可湿性粉末、颗粒或者粉剂，通过与多种惰性物质混合施用，惰性物质诸如无机矿物质(矿页硅酸盐、碳酸盐、硫酸盐、磷酸盐等)或者植物材料(弄成粉末的玉米芯、稻皮、胡桃壳等)。制剂可以包含展布剂-粘着剂佐剂、稳定剂、其它杀虫添加剂或者表面活性剂。液体制剂可以是基于水的或者非水的并且采用诸如泡沫、凝胶、混悬液、可乳化原液等。成分可以包含流变剂、表面活性剂、乳化剂、分散剂或者聚合体。
本领域技术人员应当理解，杀虫剂的浓度会变化很大，这取决于具体制剂的性质，特别是它是否是浓缩的或者是直接使用的。杀虫剂可以以至少1％重量并且可以以100％重量存在。干制剂可以具有大约1-95％重量的杀虫剂，而液体制剂通常在液相中具有大约1-60％重量的固体。制剂通常具有大约102-104个细胞/mg。这些制剂可以以每公顷大约50mg(液体的或干的)至1kg或更多进行施用。
通过喷雾、撒粉、喷洒等可以将制剂施用于昆虫的环境中，例如土壤和树叶。
另一个递送方案是将毒素的遗传物质掺入到杆状病毒载体。杆状病毒感染特定的昆虫宿主，包括欲用毒素靶向的那些昆虫宿主。可以将带有毒素表达构建体的感染性杆状病毒引入昆虫侵害的区域，从而使被感染的昆虫中毒或者被毒死。
已知昆虫病毒或者杆状病毒可以感染并且负面影响一些昆虫。病毒对昆虫的影响是慢的，并且病毒不能够立即阻止昆虫的摄食。因此，可见病毒不是最佳的昆虫害虫控制剂。然而将毒素基因结合到杆状病毒载体中提供了一种传递毒素的有效方式。除此之外，由于不同的杆状病毒对不同的昆虫是特异的，因此有可能使用特定的毒素去选择性靶向特定的破坏性昆虫害虫。对于毒素基因特别有用的载体是核型多角体病毒。已经对使用这种病毒的转移载体进行了叙述并且现在是用于转移外来基因进入昆虫的一种选择。可以将病毒-毒素基因重组体制作成口服转移的形式。杆状病毒通常通过中肠肠粘膜感染昆虫。插入到强的病毒衣壳蛋白启动子后的毒素基因会表达并迅速杀死感染的昆虫。
除了用于本发明蛋白质毒素的昆虫病毒或杆状病毒或者转基因植物递送系统外，使用苏云金芽孢杆菌包封技术可以将蛋白质进行包封，该技术诸如但不限于美国专利号4,695,455、4,695,462、4,861,595，此处引用作为参考。用于本发明蛋白质毒素的另一个递送系统是将蛋白质配制成诱饵介质，该介质可以在地上和地下昆虫诱饵装置中使用。此种技术的例子包括但不限于PCT专利申请WO 93/23998，此处引用作为参考。
基于植物RNA病毒的系统也可以用于表达细菌毒素。在这种情况下，编码毒素的基因被插入到适当植物病毒的衣壳启动子区域，该病毒可感染目的宿主植物。然后毒素被表达，因此保护植物免受昆虫损害。基于植物RNA病毒的系统叙述于Mycogen Plant Sciences的美国专利号5,500,360和Biosource Genetics Corp.的美国专利号5,316,931和5,589,367。
除了产生经转化植物外，还有其它递送系统，其中对细菌基因进行了工程改造。例如，通过将作为食物源而吸引昆虫的分子与毒素融合在一起而构建成蛋白质毒素。在实验室纯化后，此种具有“内部”诱饵的毒性剂可以被包装在标准昆虫捕捉屋中。
突变体通过本领域众所周知的操作步骤，可以制成细菌分离株的突变体。例如，通过对分离株进行甲基磺酸乙酯(EMS)诱变可以得到不产孢子的突变型。通过本领域众所周知的操作步骤使用紫外线和亚硝基胍可以产生突变型。
此处所提到或引用的所有专利、专利申请、临时申请和公开全部整合作为参考，直至它们与本说明书的明确指导不一致的程度之内均可作为参考。
下面是阐明用于实践本发明的步骤的实施例。这些实施例不应该被解释成限制。所有的百分数是以重量计，并且所有溶剂混合物比例是以体积计，除非另外注释。
实施例1-从致病杆菌Xwi株得到TC蛋白质和基因以前证明(美国专利号6,048,838)嗜线虫致病杆菌菌株Xwi(NRRLB-21733，于1997年4月29日保藏)能够产生对鞘翅目(Coleoptera)、鳞翅目(Lepidoptera)、双翅目(Diptera)和蜱螨目(Acarina)的多个目的昆虫具有口服杀虫活性的胞外蛋白质。下面公开了来源于菌株Xwi的全长基因和TC蛋白质序列。用于得到它们的方法在同时申请的题目为“用于病虫害防治的致病杆菌属TC蛋白质和基因”的Bintrim的美国临时申请(系列号60/441,717)中充分讨论。这些序列(包括N-端和内部肽序列(SEQ ID NO1-5))在序列简述部分也进行了概述。
总之，从菌株Xwi中得到39,005bp的基因组DNA片段并将其克隆到粘粒的pDAB2097中。使用Vector NTITM Suite(Informax，Inc.NorthBethesda，MD，USA)分析粘粒插入片段(SEQ ID NO6)的序列以证实所编码的ORF(可读框)。鉴定出6个全长ORF和一个部分ORF(图1和表4)。
*(C)定义为SEQ ID NO6的互补链将鉴定出的ORF的核苷酸序列和由这些ORF编码的推导氨基酸序列用于查询位于美国国家生物技术信息中心(National Center forBiotechnology Information)的数据库，查询时通过使用BLASTn，BLASTp和BLASTx通过用于进行BLAST的ncbi/nih的官方(“.gov”)网站进行。这些分析表明在pDAB2097插入片段中鉴定出的ORF同先前在发光光杆状菌和嗜线虫致病杆菌中鉴定出来的基因具有明显氨基酸序列同一性(表5)。值得注意的是GenBank登录号AJ308438中的xpt基因序列是从表达口服杀昆虫活性的重组粘粒中得到的。
*推导的氨基酸位置与数据库序列具同一性由于ORF2，ORF4，ORF5，ORF6和ORF7表现出与先前鉴定的基因具有至少95％的氨基酸序列同一性，因此在对从pDAB2097插入序列中鉴定的ORF进行进一步研究时采用相同的基因名称(表6)。
如本申请中所使用，例如XptA2表示蛋白质，例如xptA2表示基因。此外，用于分离基因和蛋白质的来源分离株用下角标表示。这种表示的例子见表6。
实施例2-来源于光杆状菌属和致病杆菌属的毒素复合体基因的异源表达开展了光杆状菌属和致病杆菌属基因在大肠杆菌中表达的一系列实验。表明将tcdA或者xptA2基因与tcdB1，tccC1，xptB1和xptC1基因特异的联合进行共表达导致在抗敏感昆虫的生物测定法中具有显著活性。此处还证明光杆状菌属基因tcdA和tcdB与致病杆菌属基因xptB1的表达导致抗南方玉米根叶甲(Diabrotica undecimpunctata howardii)的显著活性。同样，致病杆菌属xptA2与光杆状菌属tcdB1和tccC1的表达产生抗美洲棉铃虫(Helicoverpa zea)的活性。
采用了两个大肠杆菌系统来测试光杆状菌属和致病杆菌属基因。第一个依赖于存在于表达载体pBT-TcdA中的大肠杆菌启动子(图2)。构建有多达三个基因的多顺反子排列的几个质粒被构建了。每一个基因含有一个单独的核糖体结合位点和起始密码子、编码序列和终止密码子。第二个系统是通过强的T7噬菌体启动子和T7RNA聚合酶介导的(图3，pET；图4，pCot)。相似地，在一些构建体中使用了编码序列的多顺反子排列。在其它实验中，相容的质粒用于共表达。叙述实验中使用的构建体的示意图示于图5和6。
pBT-TcdA的构建表达质粒pBT-TcdA包含质粒pBC KS+(Stratagene)的复制和抗生素选择组分和质粒pTrc99a(AmershamBiosciences Corp.，Piscataway，N.J.)的表达组分(即多克隆位点上游的大肠杆菌强启动子，lac操纵子阻遏物和操纵基因)。使用体外诱变将pBCKS+的氯霉素抗性基因中的NcoI酶切位点去除。此中修饰不改变氯霉素乙酰转移酶蛋白质的氨基酸序列。如先前所述(WO 98/08932实施例27，源于光杆状菌属的杀昆虫蛋白质毒素)，使用PCR方法改造5′和3′末端将TcdA编码序列(GenBank登录号AF188483；此处作为SEQ ID NO21再现)进行修饰。随后将修饰的编码序列克隆到pTrc99a中。通过将pTrc-TcdA的钝末端Sph I/Pvu I片段与pBC KS+的钝末端Asn I/Pvu I片段连接产生质粒pBT-TcdA。结果得到质粒pBT-TcdA(图2和5)。
pBT-TcdA-TcdB的构建使用下面的引物将TcdB 1的编码序列(GenBank登录号AF346500；此处作为SEQ ID NO22再现)从质粒pBC-AS4(R.ffrench-Constant University of Wisconsin)扩增5′ATATAGTCGACGAATTTTAATCTACTAGTAAAAAGGAGATAACCATGCAGAATTCACAAACATTCAGTGTTACC 3′(SEQ ID NO23)该引物没有改变蛋白质编码序列并且在5′非编码区添加了Sal I和Spe I位点。使用的反向引物为5′ATAATACGATCGTTTCTCGAGTCATTACACCAGCGCATCAGCGGCCGTATCATTCTC 3′(SEQ ID NO24)再一次没有改变蛋白质编码序列但是在3′非编码区添加了一个Xho I位点。将扩增产物克隆到pCR2.1(Invitrogen)中并且测定了DNA序列。在预期的序列中出现了2个改变，在正向引物Spe I位点中一个A的删除(除去该Spe I位点)并且在对应于氨基酸位置1041处出现由A到T的替代，这导致了由Asp到Glu的保守替代。对这两个变化没有校正。用XhoI和Pvu I消化质粒pBT-TcdA(在TcdA编码序列的3′末端进行酶切)。用Sal I和Pvu I酶切质粒pCR2.1-TcdB1。将片段连接起来并且分离了pBT-TcdA-TcdB1重组体(图5)。Xho I和Sal I的末端是相容的，但在连接后该两个位点均消除了。质粒编码多顺反子TcdA-TcdB1 RNA。每个编码区带有单独的终止密码子和起始密码子，并且每一个的前面都是单独的核糖体结合位点。
pDAB3059的构建将TccC1蛋白质的编码序列(GenBank登录号AAC38630.1；此处作为SEQ ID NO25再现)从含有三基因Tcc操纵子的pBC KS+载体(pTccC chl；源于R.ffrench-Constant，the University ofWisconsin)扩增。正向引物为5′GTCGACGCACTACTAGTAAAAAGGAGATAACCCCATGAGCCCGTCTGAGACTACTCTTTATACTCAAACCCCAACAG3′(SEQ ID NO26)该引物不改变tccC1基因的编码序列，但提供了5′非编码的Sal I和Spe I位点和核糖体结合位点以及ATG起始密码子。反向引物为5′CGGCCGCAGTCCTCGAGTCAGATTAATTACAAAGAAAAAACTCGTCGTGCGGCTCCC3′(SEQ ID NO27)该引物也没有改变tccC1编码序列，但是提供了3′NotI和Xho I克隆位点。在用EPICENTRE FailSafe PCR试剂盒(EPICENTRE；Madison，WI)的成分扩增之后，将经过工程改造的TccC1编码序列克隆到pCR2.1-TOPO(invitrogen)中。通过5′Sal I和3′Not I酶切位点，将编码序列从pCR2.1上酶切下来并且转移到经改进的pET载体中(Novagen；Madison WI)。pET载体包含赋予壮观霉素/链霉素抗性的基因并且具有经修饰的多克隆位点。使用pTccC chl质粒DNA作为模板校正DNA测序发现的PCR-导入的突变，并且包含正确编码区的质粒命名为pDAB3059。双链DNA测序证实突变已被校正。
pBT-TcdA-TccC1的构建用XhoI酶切质粒pBT-TcdA DNA，并且将其与用Sal I和Xho I酶切的pDAB3059DNA连接。随后将tccC1基因连接到tcdA基因的下游产生pBT-TcdA-TccC1(图5)。
pBT-TcdA-TcdB1-TccC1的构建用Xho I酶切质短pBT-TcdA-TcdB1 DNA，并将其与用Sal I和Xho I酶切的pDAB3059 DNA连接。筛选出tccC1基因插入tcdB基因后面的重组体，产生了质粒pBT-TcdA-TcdB1-TccC1(图5)。
pBT-TcdA-TcdB1-XptB1的构建用Xho I酶切质粒pBT-TcdA-TcdB1 DNA，并将其与用SalI和Xho I酶切的pET280-XptB1DNA进行鸟枪法连接。如果重组体经鉴定为XptB1编码区插入到了pBT-TcdA-TcdB1的Xho I酶切位点，则产生质粒pBT-TcdA-TcdB1-XptB1(图5)。
pET28-TcdA的构建对于该质粒的叙述可见于别处，如WO98/08932实施例27，从光杆状菌属来源的杀昆虫蛋白质毒素。
pCot-TcdB1的构建将用Xho I和Sal I酶切的质粒pCR2.1-TcdB1连接到T7表达质粒pCot-3的Sal I酶切位点(图4)。质粒pCot-3具有一个pACYC的复制起点，这使得其能够与具有ColE1起源的质粒(pBR322衍生物)相容。此外，它携带有氯霉素抗生素抗性标记基因和用于表达插入到多克隆位点的编码区的T7 RNA聚合酶特异的启动子。
pCot-TccC1-TcdB1的构建用Spe I和Not I将TccC1编码区从pDAB3059 DNA上酶切下来，并且连接到质粒pET280-K(多克隆位点被替换的经修饰pET28)的多克隆位点。这导致在Spe I位点上游得到Swa I位点而在Not I位点下游得到一个Xho I位点。用Swa I和Xho I酶切质粒pET280-K-TccC1DNA以释放TccC1编码区，然后将其连接到质粒pCot-3-TcdB1的Swa I and SalI位点，从而产生质粒pCot-3-TccC1-TcdB(图6)。
pET280-XptA2，pET280-XptC1和pET280-XptB1的构建对于XptA2，XptC1和XptB1蛋白质的编码序列可以分别通过从pDAB2097通过PCR扩增得到，所述pDAB2097是含有编码这些蛋白质的三个基因的重组粘粒。用于扩增这些编码序列的PCR引物对列于表7。在所有这些引物对中，正向引物不改变基因的编码序列但提供了5′非编码的Sal I和XbaI位点以及核糖体结合位点。反向引物也不改变相应的编码序列，但提供了3′Xho I克隆位点。在用EPICENTRE Fail Safe PCR试剂盒成分扩增之后，将经过工程改造的XptA2，XptC1和XptB1的每一个克隆到pCR2.1中。已克隆的扩增产物经过测序证实保证PCR诱导的突变没有改变编码序列。将含有XptA2，XptC1和XptB1编码序列没有改变的重组质粒鉴定出来并分别命名为pDAB3056，pDAB3064和pDAB3055。通过5′Xha I和3′Xho I位点，将每一个编码序列从pCR2.1衍生物上酶切下来并转移到经过修饰的pET载体(pET280-SS是一个pET28衍生物，它具有被替换的多克隆位点，并且将链霉素/壮观霉素抗性基因插入到主链上以提供选择标记[图3])上，以产生质粒pET280-XptA2，pET280-XptC1和pET280-XptB1。
*引物中的下划线序列对应着蛋白质编码序列pET280-XptA2-XptC1的构建用Xho I酶切质粒pET280-XptA2 DNA并将其连接到pDAB3064的单一Sal I位点。所得到的连接产物含有pCR2.1和pET280-SS两个载体的主链，并且通过联合使用链霉素(25μg/mL)，壮观霉素(25μg/mL)和氨苄青霉素(100μg/mL)的抗生素选择法将其回收。用Xho I消化所回收质粒的DNA以检查片段的方向。得到了XptC1编码区刚好处于XptA2编码区下游的质粒，并且用Xho I消化DNA以去除pCR2.1载体主链。含有pET280-SS载体主链和XptA2和XptC1编码序列的所得构建体自我连接产生pET280-XptA2-XptC1。
pET280-XptC1-XptB1的构建用Xho I酶切质粒pET280-XptC1DNA并将其连接入pDAB3055的SalI单一酶切位点。所得到的连接产物含有pCR2.1和pET280-SS两个载体的主链，并且通过联合使用链霉素(25μg/mL)，壮观霉素(25μg/mL)和氨苄青霉素(100μg/mL)的抗生素选择法将其回收。用Xho I消化所回收质粒的DNA以检查片段的方向。得到了XptB1编码区刚好处于XptC1编码区下游的质粒，并且用Xho I消化DNA以去除pCR2.1载体主链。含有pET280-SS载体主链和XptC1和XptB1编码序列的所得构建体自我连接产生pET280-XptC1-XptB1。
pET280-XptA2-XptB1的构建用Xho I酶切质粒pET280-XptA2DNA并将其连接入pDAB3055的Sa1I单一酶切位点。所得到的连接产物含有pCR2.1和pET280-SS两个载体的主链，并且通过联合使用链霉素(25μg/mL)，壮观霉素(25μg/mL)和氨苄青霉素(100μg/mL)的抗生素选择法将其回收。用Xho I消化所回收质粒的DNA以检查片段的方向。得到了XptB1编码区刚好处于XptA2编码区下游的质粒，并且用Xho I消化DNA以去除pCR2.1载体主链。含有pET280-SS载体主链和XptA2和XptB1编码序列的所得构建体自我连接产生pET280-XptA2-XptB1。
pET280-XptA2-XptC1-XptB1的构建用Xho I酶切质粒pET280-XptA2-XptC1 DNA并将其连接入pDAB3055的Sal I单一酶切位点。所得到的连接产物含有pCR2.1和pET280-SS两个载体的主链，并且通过联合使用链霉素(25μg/mL)，壮观霉素(25μg/mL)和氨苄青霉素(100μg/mL)的抗生素选择法将其回收。用Xho I消化所回收质粒的DNA以检查片段的方向。得到了XptB1编码区刚好处于XptC1编码区下游的质粒，并且用Xho I消化DNA以去除pCR2.1载体主链。含有pET280-SS载体主链和XptA2，XptC1和XptB1编码序列的所得构建体自我连接产生pET280-XptA2-XptC1-XptB1。
基于pBT的构建体的表达将pBT表达质粒转化大肠杆菌菌株BL21细胞并且涂板于含有50μg/mL氯霉素和50mM葡萄糖的LB琼脂上，并且转化体在37℃下生长过夜。将大约10-100个分离较好的克隆接种到500mL带挡板摇瓶的200mL含有50μg/mL氯霉素和75μM异丙基硫代-β-D-半乳糖吡喃糖苷(IPTG)的无菌LB中。在28℃下以200转每分振荡培养24小时。通过离心(大约3000×g)收集细胞并将细胞以30-120 OD600单位/mL的细胞密度悬浮于磷酸盐缓冲液中(30mM，pH7.4；NutraMax；Gloucester，MA)。然后将稀释的细胞用于昆虫的生物测定。
备选地，将生长24小时的细胞置于冰上冷却，并且用磷酸盐缓冲液调整为20-30 OD600单位/mL。使用探头超声破碎仪(Soniprep 150，MSE)，并在1/3体积的0.1mm的玻璃珠(Biospec；Bartletsville，OK)中以20微米的振幅进行2×45秒脉冲。在Eppendorf微离心管中以14,000转每分离心10分钟使裂解物变得澄清。将澄清的裂解物用UltraFree 100kDaunits(Millipore；Bedford，MA)进行浓缩、收集并用磷酸盐缓冲液调节至10mg/mL，并用于昆虫生物测定法。
基于T7的构建体的表达除了将其转化进入T7表达菌株BL21(DE3)(Novagen，Madison，WI)和联合使用链霉素(25μg/mL)和壮观霉素(25μg/mL)作为抗生素选择以外，将基于T7的表达质粒进行上述的同pBT表达质粒相同的操作。
实施例3-异源表达的毒素复合体基因的杀昆虫生物测定结果如上所述，使用pBT表达系统开展了一系列的表达实验。将大肠杆菌进行转化、诱导和在28℃下过夜生长。将细胞进行收集、洗涤和标准化成相同的浓度并且将其作为南方玉米根叶甲的食物进行应用并进行生物测定。如表8所示，只有当所有三个光杆状菌属基因tcdA，tcdB1和tccC1在同一细胞中表达时，才观察到明显的死亡。基因的其它组合不能够导致明显的死亡。例如，表8表明tcdB1和tccC1基因的特定组合未表现出杀昆虫的活性。当在pBT-TcdA-TcdB1-TccC1质粒上的基因表达时，通常观察到死亡，并且在这些实验中(表8)在应用于昆虫食物之前将细胞在4℃储存24小时没有明显降低或提高死亡率。如果在共表达质粒pBT-TcdA-TcdB1-TccC1上的基因之后将细胞进行裂解也观察到南方玉米根叶甲的死亡。如果将裂解液在-70℃冻存一周，活性没有降低(表9)。
将完整大肠杆菌细胞用磷酸盐缓冲液洗涤、浓缩并调整至相同的细胞浓度，并且应用到昆虫的食物中。第1天的样品是立即测定的。第2天的样品是相同的细胞制品，但在应用于昆虫食物之前在4℃过夜储存了。
等级划分代表南方玉米根叶甲的死亡百分数％(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＝81-100％)。
将大肠杆菌细胞用磷酸盐缓冲液洗涤、浓缩并调整至相同的细胞浓度，并且应用到昆虫的食物制剂中。可选择地，裂解液用超声进行制备，并将新鲜制备的裂解液应用到食物中或者在-70℃冻存7天后再应用到食物中。
等级划分代表南方玉米根叶甲的死亡百分数％(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＝81-100％)。
在另一系列实验中，致病杆菌属xptB1基因代替了光杆状菌属tccC1基因，并且作为质粒pBT-TcdA-TcdB1-XptB1的多顺反子操纵子的一部分进行了表达。这些实验证明致病杆菌属xptB1基因能够代替光杆状菌属tccC1基因，在完整大肠杆菌细胞生物测定中导致了南方玉米根叶甲的死亡(表10)。
将完整大肠杆菌细胞用磷酸盐缓冲液洗涤、浓缩并调整至相同的细胞浓度，并且应用到昆虫的食物制剂中。
等级划分代表南方玉米根叶甲的死亡百分数％(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＝81-100％)。
多种光杆状菌属基因从分别的质粒上进行表达也导致南方玉米根叶甲的死亡。当将存在于pET表达质粒上的tcdA和存在于相容表达载体pCot-3上的tccC1和tcdB1基因与这些质粒的对照组合相比较时可以观察到明显的活性(表11)。如上面所提到，tcdB1和TccC1基因单独存在时不能导致明显的活性(表11)。
将完整大肠杆菌细胞用磷酸盐缓冲液洗涤、浓缩并调整至相同的细胞浓度，并且应用到昆虫的食物制剂中。
等级划分代表南方玉米根叶甲的死亡百分数％(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＝81-100％)。
异源表达的致病杆菌属毒素复合体基因的生物测定结果如上所述使用pET表达系统开展了一系列表达实验。将大肠杆菌细胞进行转化、诱导并在28℃下生长过夜。将细胞收集、洗涤、并标准化成相同的浓度，并测试对欧洲玉米螟(Ostrinia nubilalis)(ECB)，棉铃虫(CEW)和烟夜蛾(TBW)的杀昆虫活性。如表12所示，当xptA2，xptC1和xptB1存在于同一个构建体中时可观察到最大水平的杀昆虫活性。
将完整大肠杆菌细胞用磷酸盐缓冲液洗涤、浓缩并调整至相同的细胞浓度，并且应用到昆虫的食物制剂中。
等级划分代表与对照相比的生长抑制百分数％(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＝81-100％)。
异源表达的xptA2，tcdB1和tccC1的生物测定结果用表13列出的pET280和pCoT构建体共转化大肠杆菌细胞。如上所述对转化体进行诱导、处理和生物测定。在这些测定中，含有pCOT/pET280-XptA2-XptC1-XptB1或者pCoT-TcdB1-TccC1/pET280-XptA2质粒组合的共转化体表现出最高水平的杀昆虫活性。这些实验表明光杆状菌属tcdB1和tccC1基因即使相对于xptA2反式存在，也能够代替致病杆菌属xptC1和xptB1基因，这导致质量上相似水平的增强了的杀昆虫活性。
将完整大肠杆菌细胞用磷酸盐缓冲液洗涤、浓缩并调整至相同的细胞浓度，并且应用到昆虫的食物制剂中。
等级划分代表相对于对照的生长抑制百分数％(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＝81-100％)。
实施例4-致病杆菌属XptA2毒素与类芽孢杆菌DAS1529株TC蛋白质互补该实施例提供了关于共同未决的美国临时申请系列号60/392,633的额外的数据，其在上面的背景部分进行了讨论。该数据与本申请相关，因为它为类芽孢杆菌DAS1529株TC蛋白质(此处作为单一操纵子表达)能够补偿例如源于嗜线虫致病杆菌Xwi的XptA2毒素(见SEQ ID NO34)的能力提供了实验证据。开展了独立表达DAS1529TC操纵子和XptA2或者在同一大肠杆菌细胞中共表达XptA2基因和TC操纵子的两个独立实验。表达不同毒素/毒素组合的完整细胞用于测试抗两种鳞翅昆虫美洲棉铃虫(Heliothis zea；CEW)和美洲烟夜蛾(Heliothis virescens；TBW)的活性。从此两个实验得到的数据表明DAS1529TC蛋白质能够增强致病杆菌属XptA2抗所测试昆虫的活性。
A.共表达DAS1529TC蛋白质和致病杆菌属TC XptA2毒素DAS 1529TC操纵子的表达受pET101.D表达载体上的T7启动子/lac操纵基因控制，该表达载体携带有ColEl复制起点和氨苄青霉素抗性选择标记(Invitrogen)。在美国系列号60/392,633的实施例8中可以找到关于类芽孢杆菌属TC操纵子的克隆和表达的详细叙述。将XptA2基因克隆进入pCot-3表达载体，该载体携带有氯霉素抗性选择标记和与ColEl相容的复制起点。pCot-3载体表达系统也受T7启动子/lac操纵基因的调节。因此，相容的复制起点和不同的选择标记形成了TC操纵子和XptA2在同一大肠杆菌细胞中共表达的基础。将携带有TC操纵子和XptA2的质粒DNA单独地或者组合在一起进行转化大肠杆菌BL21starTM(DE3)。在含有针对pET101.D-TC操纵子的50μg/ml羧苄青霉素、针对pCot-3-XptA2的50μg/ml氯霉素和针对pET101.D-TC操纵子/pCot-3-XptA2的两种抗生素的LB琼脂平板上筛选转化体。为了抑制毒素的基础表达，在琼脂LB培养板和液体LB培养基中含有终浓度为50mM的葡萄糖。
对于毒素的生产，将生长在LB琼脂平板上的过夜培养物接种到5mL和50mL含有抗生素和50mM葡萄糖的LB培养基中。将培养物在30℃、300转每分的摇动器中进行生长。一旦培养物浓度于600nm达到O.D.约为0.4时，将终浓度为75μM的IPTG加入到培养基中以诱导基因表达。24小时之后，收集大肠杆菌细胞通过NuPAGE系统(Invitrogen)进行蛋白质凝胶分析。将0.5mL1×培养液中的细胞沉淀重悬于100μL1×NuPAGELDS样本缓冲液中。在短暂的超声裂解和5分钟的煮沸后，将5μL的样品加到4-12％NuPAGEbis-tris梯度凝胶上用于进行总蛋白质分布分析。当XptA2单独表达时或者在TC操纵子存在下表达时，XptA2的表达达到了可检测水平。基于通过Personal Densitometer SI(Molecular Dynamics)的凝胶扫描分析，当与TC操纵子共表达时，XptA2的表达几乎是自身表达的8倍。对于5mL的诱导实验，XptA2的表达几乎相等。
B.杀昆虫活性的生物测定如在美国系列号60/392,633的实施例8所述，当DAS1529TC ORF单独表达或者作为一个操纵子表达时，没有抗TBW和CEW的活性。下面的生物测定实验集中在确定类芽孢杆菌属(DAS1529)TC蛋白质(ORF3-6；TcaA-，TcaB-，TcaC-和TccC样蛋白质；见SEQ ID NO35-43)是否能够增强致病杆菌属TC蛋白质(以XptA2为例)活性。在5mL诱导实验中制备了完整大肠杆菌细胞的4×细胞浓缩液作为生物测定样品，XptA2和XptA2/TC操纵子的细胞含有的XptA2非常低但是几乎是等量的。表14中的数据显示在4×细胞浓度下，类芽孢杆菌属TC蛋白质(在表14中为“TCs”)+XptA2的组合对于抗CEW是有效的。这表明类芽孢杆菌属(DAS1529)TCs具有对致病杆菌属XptA2的补偿效应。
*-，++，+++＝分别为没有活性、中等活性和高活性在第二个生物测定实验中，根据光密度计凝胶扫描分析将XptA2细胞和XptA2+TC操纵子细胞内的XptA2的量进行了标准化。如表15所示，XptA2本身在40×的条件下具有对TBW(H.virescens)的中度活性，但是在20×和低于20×的条件下活性降低到不可检测的水平。但是，当与类芽孢杆菌属TC蛋白质共表达时，在10×和5×XptA2存在下高水平活性十分明显，并且在1.25×XptA2时仍明显具有低的活性。这些观察表明这些DAS1529TC蛋白质对于XptA2抗烟夜蛾具有显著的增效作用。在所测试的最高剂量，阴性对照或者TC操纵子本身均没有抗该昆虫的任何活性。
*n.d.-没有测定；-，+，++，+++＝分别为没有活性、低、中等活性和高活性实施例5-伯氏致病杆菌B和C蛋白质的混合互补作用实施例5A.综述使用粘粒补偿筛选开展了对编码能够加强或者协同杀昆虫活性蛋白质光杆状菌属TcdA和致病杆菌属XptA2wi活性的因子的基因的鉴定和分离。来源于伯氏致病杆菌(菌株ILM104)粘粒基因组文库的单个大肠杆菌克隆用于制备粗细胞提取物，将这些提取物与纯化的毒素混合并进行生物测定。将裂解液与纯化的光杆状菌属毒素TcdA一起分析其抗南方玉米根叶甲幼虫的活性。同时，将裂解液与纯化的致病杆菌属XptA2wi混合并测定其抗烟夜蛾和美洲棉铃虫幼虫的活性。如果裂解液加上纯化毒素的组合具有比其单独每种成分要高的活性，则粘粒裂解液记为阳性。
对于初级筛选的样品(以96-孔的形式)的杀昆虫活性进行两次重复测试，并将所得的数值与对照进行比较。将阳性的样品进行再生长，并在二级筛选中进行测试。在初级和次级筛选中被鉴定为阳性的粘粒进行第三次筛选。较大的培养体积(见下)用于第四次筛选，在128-孔形式的生物测定中测试生物学活性。
将在本筛选中鉴定为具有增强活性的粘粒之一的DNA进行亚克隆。将仍具有活性的单个亚克隆的DNA序列进行测序并且表明其含有两个开放阅读框架，命名为xptB1xb和xptC1xb。将这些编码区亚克隆到pET质粒中并在大肠杆菌中进行了表达。当将TcdA或者XptA2wi蛋白质与共表达XptB1xb和XptC1xb的裂解液混合后可见杀昆虫活性急剧增加。当与纯化的TcdA或者XptA2wi混合后，仅含有XptB1xb或者XptC1xb的裂解液具有最小的作用。
实施例5B.杀昆虫生物测定方法杀昆虫生物测定是通过在96孔微孔板(Becton Dickinson andCompany，Franklin Lakes，NJ)或者专门设计用于杀昆虫生物测定的128孔碟子(C-D International，Pitman，NJ)中使用人工饲料进行的。将2种鳞翅目物种的卵用于在96孔微孔板中的生物测定美洲棉铃虫(Helicoverpa zea(Boddie))和美洲烟夜蛾(Heliothis virescens(F.))。新生的幼虫用于在128孔碟子中的生物测定。以这种形式测试的鳞翅目物种包括美洲棉铃虫、美洲烟夜蛾和甜菜夜蛾(Spodoptera exigua(Hubner))。一个鞘翅目物种，南方玉米根叶甲(Diabroticaundecimpunctata howardii(Barber))，也用于该种生物测定形式的测试。
在这些生物测定中记录的数据包括处理的昆虫总数、死亡昆虫数目、生长受妨碍的昆虫数目和存活昆虫的重量。在报导生长抑制的情况下，其计算如下％生长抑制＝[1-(处理昆虫的平均重量/只有载体的对照中昆虫的平均重量)]*100实施例5C.其它实验操作步骤这在由Apel-Birkhold等同时提出的申请中有更加详细的叙述，该申请题目为“来源于伯氏致病杆菌的毒素复合体的蛋白质和基因”代理号(attorney docket no.)为DAS-114P(系列号———)。
实施例5D.
已经表明质粒pDAB6026能够编码具有协同TcdA和XptA2wi的杀昆虫毒性活性的活性物质。将含有质粒pDAB6026或者pBCKS+载体对照的大肠杆菌细胞接种于200mL含有氯霉素(50μg/mL)和75μM IPTG(异丙基硫代-β-D-半乳糖吡喃糖苷)的LB中，并于28℃、180转每分的振荡条件下生长2天。然后将细胞在3500×g离心10分钟。将沉淀重悬于5mLButterfield′s磷酸盐溶液(Fisher Scientific)中，并转移至含有1.5mL直径0.1mm玻璃珠(Biospec，Bartlesville，OK，catalog number 1107901)的超声管中。用冰冷却细胞-玻璃珠混合物，然后通过超声将细胞裂解，超声时使用带有2mm探头的Branson Sonifier250(Danbury CT)在约20的输出条件下进行两次45秒的脉冲，在两次脉冲之间要完全冷却。将上清液转移至2mL微量离心管中并在16,000×g离心5分钟。然后将上清转移至15mL管中，并测定蛋白质的浓度。用H2O 1∶5稀释Bio-Rad Protein DyeAssay Reagent并取1mL加入到10μL 1∶10稀释的每个样品和浓度为5、10、15、20和25μg/mL的牛血清白蛋白(BSA)中。然后使用ShimadzuUV160U分光光度计(Kyoto，JP)记录波长595的样品的光密度。然后，对照BSA标准曲线计算出每个样品中的蛋白质量并用磷酸盐缓冲液将其调整为3-6mg/mL。在进行杀昆虫饲养生物测定法测试之间将600ngXptA2wi毒素蛋白质加入到500μL大肠杆菌裂解液中。pDAB6026和XptA2的组合表现出具有有效的活性(表16)。
实施例5E.xptB1xb和xptC1xb基因的发现、工程改造和测试将质粒pDAB6026的DNA送交SeqWright DNA Sequencing(Houston，TX)进行DNA序列的测定。发现了两个具有相当大小的完整开放阅读框(ORF)。第一个(作为SEQ ID NO48公开)与属于“B”类的已知毒素复合体基因具有明显的相似性。因此将该ORF称作xptB1xb并且其编码如SEQ ID NO49中公开的蛋白质。第二个ORF(SEQ ID NO50)编码与毒素复合体“C”蛋白质同源的蛋白质(SEQ ID NO51)并且因此而称作xptC1xb。
通过在编码区的5′和3′末端加入限制性酶切位点以及加入核糖体结合位点和最佳的翻译终止信号对xptB1xb和xptC1xb基因进行工程改造(使用聚合酶链式反应；PCR)以便于高水平的重组表达。此外，将沉默突变(氨基酸序列没有变化)引入到编码区的5′末端以便减少潜在的mRNA二级结构并因此而增加翻译。策略是在基因的5′和3′末端扩增/工程改造片段，使用“剪接重叠延伸(Splice Overlap Extensions)”反应将远端片段进行连接，然后通过限制性位点加入开放阅读框的非扩增中心部分。该方法使在DNA序列中PCR-诱导的变化的可能性最小化。将工程改造的编码区作为分开的编码区(SEQ ID NO52和SEQ ID NO53)或者作为二顺反子操纵子(SEQ ID NO54)克隆到pET表达质粒(Novagen，Madison，WI)中。表达质粒的名称示于表17。
用pET(对照)载体或者载体pDAB6031、pDAB6032或者pDAB6033的DNA转化新鲜制备的大肠杆菌T7表达菌株BL21 StarTM(DE3)(Stratagene，La Jolla，CA)感受态细胞，并将其接种于250mL含有50μg/mL氯霉素和75μM IPTG的LB中。于28℃下180转每分振荡生长24小时后，将细胞于5500×g离心10分钟。将沉淀重悬于5mL磷酸盐溶液并且转移至含有1.5mL直径0.1mm玻璃珠的50ml锥形管中，然后用上述的作为“恒量”和设定为30的两次45秒的脉冲进行超声破碎。将样品于3000×g离心15分钟，将上清转移至2mL微量离心管中，14,000转每分离心5分钟，然后将上清液转移至15mL的管中。如上所述对蛋白质浓度进行测定并且用磷酸盐缓冲液将裂解液调整至5mg/mL。将每个裂解液的一组3个样品接受杀昆虫生物测定。对于第一个样品，加入磷酸盐缓冲液以代替纯化的毒素；对于第二个样品，加入足够量的TcdA蛋白质以便在杀昆虫生物测定孔中提供50ng/cm2的剂量；并且对于第三个样品，加入足够量的XptA2wi蛋白质以便在杀昆虫生物测定孔中提供250ng/cm2的剂量。
生物测定的结果示于表18。没有添加低水平额外TcdA或者XptA2wi蛋白质的对照样品(如来源于载体、pDAB6031、pDAB6032和pDAB6033的样品)对昆虫几乎没有影响。同样，含有低水平TcdA或者XptA2wi和pDAB6031或者pDAB6032裂解液的样品具有最小的效应。相反，在包括低水平TcdA或者XptA2wi和pDAB6033裂解液的样品中观察到明显的活性。
实施例5F.伯氏致病杆菌菌株ILM104的XptB1xb和XptC1xb蛋白质的鉴定、纯化和表征生物测定驱使的含有pDAB6033的大肠杆菌裂解液的分级导致通过MALDI-TOF鉴定出两个共纯化的蛋白质XptB1xb和XptC1xb。含有该两个蛋白质的峰有效地增强TcdA和XptA2wi的活性。
根据它们协同或增强TcdA抗南方玉米根叶甲的能力或者XptA2wi抗美国棉铃虫的能力鉴定出活性级分。所有的生物测定在上面实施例5A中叙述的128孔形式中进行。
在电导22-24mS/cm洗脱的蛋白质级分中检测到2个活性的峰(峰1和峰2)。峰1和峰2增强活性的例子示于表19。对峰1和峰2进行随后的纯化和分析。
峰1和峰2的凝胶中都含有两个主要的条带，一个迁移在约170kDa并且另一个迁移在约80kDa。从峰1的凝胶中含有三个额外的蛋白质，它们迁移在大约18、33和50kDa。经过回顾分析显示在纯化的起始阶段大约170kDa和大约80kDa的条带是丰富的并且变得在每一步进行性富集。
使用MALDI-TOF质谱分析了提取的肽，在Voyager DE-STRMALDI-TOF质谱仪(PerSeptive Biosystems，Framingham，MA)产生肽质量图谱(PMF)。对从约170kDa条带中提取的样品的分析证实与XptB1xb相同。对从约80kDa条带中提取的样品的分析证实与XptC1xb相同。虽然XptC1xb蛋白质预测的分子量根据从基因序列(SEQ ID NO50)计算，分子量为108kDa，但是提取的蛋白质在SDS/PAGE中跑的明显地比预计的要快。代表着全长肽序列的肽片段的存在表明提取的蛋白质是全长的。
样品列中的数值代表应用于食物中的峰1和峰2XptB1xb/XptC1xb蛋白质的浓度(ng/cm2)。对于抗美国棉铃虫的生物测定，在生物测定中包含250ng/cm2的XptA2xi。对于抗南方玉米根叶甲的生物测定，在生物测定中包含100ng/cm2的TcdA。在每个样品中使用总共8个幼虫。
实施例6-其它混合和匹配例子在该实施例中，证明三种毒素复合体(TC)蛋白质的组合可以实现强效的昆虫抑制。当将A类蛋白质与B类和C类蛋白质混合后可以观察到令人信服的杀昆虫活性。本发明的惊人之处在于A类、B类和C类蛋白质的多种组合均导致强烈的昆虫抑制。毒素复合体蛋白质可以有广泛不同的来源并且可以与该类的其它功能成员拥有有限量的氨基酸同一性。
实施例6A.引言对于由15种不同的毒素复合体基因编码的杀昆虫和生长抑制活性分别和彼此组合进行了测试。测试了来源于所述A、B或者C类的每一类中的几个实例。基因来源于3个属(光杆状菌属，致病杆菌属和类芽孢杆菌属；两个革兰氏阴性细菌和革兰氏阳性细菌)和四个不同的种。该实施例的结果与毒素复合体A类蛋白质(例如TcdA和XptA2wi)单独具有明显的活性的观察相一致。这在最近Liu等的转基因植物(Liu，D.，Burton，S.，Glancy，T.，Li，Z-S.，Hampton，R.，Meade，T.和Merlo，D.J.″283-kDa发光光杆状菌蛋白质TcdA在拟南芥菜(Arabidopsis thaliana)中表现出对昆虫的抗性″，Nature Biotechnology 2003，10月，21卷，10期，1222-1228页)中已经证明。该结果还与下面的观察相一致，即从同一个操纵子、菌株或者属共表达的三个毒素复合体基因(A类、B类和C类)导致比单独A类基因、或者该三类的任何一个或者两个组合的杀昆虫活性都要大(Hurst，M.，Glare，T.，Jackson，T.和Ronson，C.″嗜线虫沙雷菌的质粒定位的病原决定子，Grass Grub Amber病的诱发因素，表现出同发光光杆状菌杀昆虫毒素的相似性″，Journal ofBacteriology，2000年9月，182卷，18期，5127-5138页；Morgan，J.A.，Sergeant，M.，Ellis，D.，Ousley，M.和Janett，P.″嗜线虫致病杆菌PMFI 296来源的杀昆虫基因的序列分析。″Appliedand Environmental Microbiology，2001年5月，2062-2069页，67卷，5期；Waterfield，N.，Dowling，A.，Sharma，S.，Daborn，P.，Potter，U.和Ffrench-Constant，R.″大肠杆菌中发光光杆状菌W-14毒素复合体的口服毒性″，Applied and Environmental Microbiology，2001年11月，67卷，11期，5017-5024页)。
令人惊奇地，下面的数据证明了这样一种发现，即毒素复合体A类蛋白质可以与例如从大肠杆菌制备的表达具有广泛不同来源的B类和C类基因的裂解液进行混合或者匹配，以产生极好的杀昆虫活性或者昆虫生长抑制活性。例如，来源于致病杆菌属的A类蛋白质可以与表达光杆状菌属来源的B类基因和类芽孢杆菌属来源的C类基因的裂解液进行混合以提供杀昆虫活性的组合。同样，来源于光杆状菌属的A类蛋白质可以与表达致病杆菌属来源的B类和C类的裂解液混合，反之亦然。许多组合是可能的；下面所示的许多会导致强效的杀昆虫活性。不可预计的新发现是来源于以对鞘翅目具活性的菌株(发光光杆状菌W-14株)或者对鳞翅目具活性的菌株(嗜线虫致病杆菌菌株Xwi)的毒素复合体A、B和C类的成分可以功能性的混合和匹配。另一个另人惊奇的是对于单个A、B或者C蛋白质的可能的差异程度的发现。例如，能与B类/C类功能性结合的单个A类蛋白质(例如TcdA和XptA2wi)彼此只享有41％的氨基酸同一性。同样，任何一个单个B类蛋白质可以只与另一个功能性B类蛋白质享有41％的同一性。相似地，任何给定的C类蛋白质可与另一个C类蛋白质只享有35％的同一性。
实施例6B.蛋白质来源和构建A类蛋白质TcdA和XptA2wi以从异源表达所述蛋白质的荧光假单胞菌的培养物中制备的纯化形式使用。从其它异源来源(植物；细菌)的TcdA和XptA2wi的制品在测定中是功能等效物。B类和C类蛋白质作为大肠杆菌裂解液的成分进行测试。通过与纯化的几种B类和C类组合的制品相比较，确认为裂解液的用途。通过将编码B类和C类蛋白质的阅读框克隆进入pET载体(Novagen，Madison WI)改造成能够在大肠杆菌中进行表达。每一个编码区含有一个具有恰当间隔的核糖体结合位点(相对于起始密码)和终止信号。将一些基因的5′端DNA序列进行修饰以减少预测的RNA二级结构并因此而提高翻译。这些碱基的变化是沉默的并且不导致蛋白质中氨基酸的变化。在B类基因与C类基因一起用于测试的情况下，在pET表达质粒中构建了这样的操纵子，其中B类的编码序列首先被转录然后是C类编码序列的转录。通过一个接头序列将两个编码区分隔开，此接头序列含有一个与C类蛋白质编码区的起始密码子有恰当间隔的核糖体结合位点。在双顺反子构件中编码区之间的DNA序列以5′-3′方向显示。表20-27包含由多种表达质粒编码的蛋白质、编码区来源和质粒参考编号的目录。表22B、23B和28-31显示了表达质粒中使用的接头序列。
实施例6C.表达条件和裂解液制备使用标准方法将表20-27中所列的pET表达质粒转化大肠杆菌T7表达菌株BL21(DE3)(Novagen，Madison WI)或者BL21 StarTM(DE3)(Stratagene，La Jolla，CA)。将10-200个新鲜转化的克隆接种到250mL含有50μg/ml抗生素和75μM IPTG的LB中开始进行表达培养。将培养物于28℃下、180-200转每分生长24小时。通过在250ml Nalgene瓶中于4℃、3,400×g离心10分钟收集细胞。将沉淀悬浮于4-4.5mLButterfield′s磷酸盐溶液(Hardy Diagnostics，Santa Maria，CA；0.3mM磷酸钾pH 7.2)。将悬浮的细胞转移至含有1mL0.1mm直径玻璃珠(Biospec，Bartlesville，OK，目录号1107901)的50mL带螺旋帽的聚丙烯离心管中。将细胞-玻璃珠混合液置冰上冷却，然后将细胞通过超声破碎裂解，超声时使用BransonSonifier 250(Danbury CT)在约20的输出条件下使用2mm探头进行两次45秒的脉冲，在两个脉冲之间要完全冷却。将裂解液转移至2mL Eppendorf管中并且在16,000×g下离心5分钟。收集上清液并且测定蛋白质的浓度。用H2O将Bio-Rad Protein Dye AssayReagent以1∶5进行稀释，并且将1mL加入到10μl 1∶10稀释的每一个样品中和加入到浓度为5、10、15、20和25μg/mL的牛血清白蛋白(BSA)中。然后用分光光度计法读取在Shimadzu UV160U分光光度计上(Kyoto，JP)上测量的波长595nm的吸光度。然后根据BSA的标准曲线计算出每个样品的蛋白质的量并且用磷酸盐缓冲液调整至3-6mg/mL。一般地将新鲜制备的裂解液进行测定，然而当储存于-70℃后没有观察到活性的损失。
实施例6D.生物测定条件杀昆虫生物测定通过在特别设计用于杀昆虫生物测定法的128孔碟子(C-D International，Pitman，NJ)中人工饵料上的新生幼虫进行。所测定的物种有南方玉米根叶甲、美洲棉铃虫、美洲烟夜蛾和甜菜夜蛾。
生物测定法是通过在控制的环境条件下(28℃，～40％r.h.，168[LD])孵育5天，此时记录处理的昆虫的总数、死亡昆虫数和存活昆虫的重量。对于每一个处理计算死亡的百分数和生长抑制的百分数。生长抑制的计算如下％生长抑制＝[1-(处理的昆虫的平均重量/只有载体的对照中昆虫的平均重量)]*100在处理的昆虫的平均重量大于只有载体的对照中昆虫的平均重量的情况下，生长抑制记录为0％。
仅用粗裂解液或者与加入的TcdA或者XptA2wi毒素蛋白质一起的生物学活性的测定如下。将对照培养物或者那些表达增效剂蛋白质的大肠杆菌粗裂解液(40μL)应用在生物测定碟的8个孔中的人工饵料的表面。在每个孔中受处理食物的平均表面积为大约1.5cm2。将裂解液调整到2-5mg/mL总蛋白质并且与或不与TcdA或者XptA2wi一起应用。所加入的TcdA或者XptA2wi是来自异源表达所述蛋白质的细菌培养物的高度纯化级分。食物中TcdA和XptA2wi的终浓度分别为250ng/cm2和50ng/cm2。
生物测定的结果总结于表32-39中。当用工程改造只表达B类或者C类蛋白质的大肠杆菌克隆的裂解液喂食幼虫时可观察到对所测试的昆虫物种的存活或生长有很小作用至没有作用(表32和33)。同样，在没有纯化毒素存在的情况下用B类和C类蛋白质的组合喂食幼虫时观察到很小的作用至没有作用(表34-39，“无”列)。当用工程改造表达B类和C类蛋白质组合的克隆的大肠杆菌裂解液与纯化的毒素喂食幼虫时通常可观察到对生存和/或生长明显的影响(表2C-2H，“TcdA”和“XptA2wi”列)。所测试的B类和C类的组合与纯化的TcdA一起最典型的是对南方玉米根叶甲造成影响而所测试组合与纯化的XptA2wi一起主要是对3个鳞翅目物种之一造成影响，且美洲棉铃虫是一贯最敏感的物种。应当指出许多B类和C类的组合产生XptA2wi针对南方玉米根叶甲的可观察的效应。相反，这些组合会产生TcdA对鳞翅类物种的可观察的效应是不正确的。
表32-39表明喂食给昆虫幼虫单独的大肠杆菌裂解液和与光杆状菌属或者致病杆菌属毒素蛋白质的组合的生物学活性。在每一大肠杆菌克隆中包含的基因对应于表20-27中包含的那些基因。使用下面的等级将生物学活性进行分类0＝平均死亡率＜50％并且平均重量＞空载体/无毒素处理的平均重量的50％，+＝平均重量≤空载体/无毒素处理的平均重量的50％，++＝平均死亡率≥50％或者平均重量≤空载体/无毒素处理的平均重量的20％，和+++＝平均死亡率≥95％或者平均重量≤空载体/无毒素处理的平均重量的5％。
实施例7-TC蛋白质的其它混合和匹配为了证明当前发现的TC蛋白质的多功能性，采用双质粒表达系统开展了额外的大肠杆菌表达实验。基于T7启动子的系统利用pACYC衍生物(称作pCot-3或者4，氯霉素抗性)表达TcdA或者XptA2蛋白质，而相容的T7启动子pET280质粒(卡那霉素抗性)表达TcdB1(SEQ IDNO22)、TcdB2(SEQ ID NO45)、XptC1(SEQ ID NO18)、TccC1(SEQ ID NO25)、TccC3(SEQ ID NO47)和XptB1(SEQ ID NO16)蛋白质的多种组合，所有这些均存在于同一个细胞。同样在另一系列实验中，利用不同pACYC衍生物(称作pCTS，壮观霉素/链霉素抗性)的大肠杆菌启动子系统用来表达TcdA(SEQ ID NO21)或者XptA2(SEQ IDNO34)蛋白质，而相容的pBT280质粒(氯霉素抗性)表达TcdB1、TcdB2、XptC1、TccC1、TccC3和XptB1的多种组合。当进行生物测定时发现，两个系统产生相似活性的蛋白质。
通过首先制备含有pCot-3、pCot-TcdA或者pCot-XptA2的感受态BL21(DE3)细胞原种进行基于T7启动子的实验。用对照pET280质粒或者存在于pET280载体中的上面提到的TC基因的任何组合转化这些细胞。在含有氯霉素和卡那霉素的培养基上对含有两种质粒的细胞进行选择。相似地，对于大肠杆菌启动子的系统，制备了含有pCTS、pCTS-TcdA或者pCTS-XptA2的感受态BL21细胞。然后用pBT280对照质粒或者存在于pBT280载体中的上面提到的任何TC组合转化感受态细胞。当在一个特定质粒中存在多于一个的TC基因时，它们则作为在5′末端具有单一一个启动子的两个基因操纵子而排列。在第一个编码区后跟着翻译终止信号；一个分开的核糖体结合位点(SD序列)和翻译起始信号用来起始第二个编码区的翻译。实施例2和3中叙述的方法用于表达培养物的生长、裂解液的制备和杀昆虫活性的评价。在一些实验中使用了改良的测定方法，其中将蛋白质TcdA和XptA2的浓缩制品添加到含有TcdB1、TcdB2、XptC1、TccC1、TccC3和XptB1的单一或组合的裂解液中(表40和41)。
裂解完整的大肠杆菌细胞并在实验中将可溶的蛋白质通常规一化至5-10mg/ml。通过加至昆虫食物上如所述进行裂解物的生物测定。级别划分为以相对于对照的生长抑制百分数％表示(0＝0-25％；+＝26-50％；++＝51-65％；+++＝66-80％；++++＝81-95％；+++++＝96-100％)。
裂解完整大肠杆菌细胞并将可溶蛋白质调整到8-15mg/ml的同一样品浓度。*当应用到昆虫食物制品的表面时，TcdA蛋白质以50ng/cm2的终浓度加入到样品中。
等级范围以饲喂添加TcdA毒素蛋白质的处理的存活昆虫相对于饲喂没有添加TcdA毒素蛋白质的处理存活昆虫的生长抑制％表示(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＞80％)。
裂解完整的大肠杆菌细胞并将可溶蛋白质调整到8-15mg/ml的同一样品浓度。*当应用到昆虫食物制品的表面时，XptA2蛋白质以250ng/cm2的终浓度加入到样品中。
等级范围以饲喂添加XptA2毒素蛋白质的处理的存活昆虫相对于饲喂没有添加XptA2毒素蛋白质的处理存活昆虫的生长抑制的％表示(0＝0-10％；+＝11-20％；++＝21-40％；+++＝41-60％；++++＝61-80％；+++++＞80％)。
实施例8-混合和匹配测定和序列关系的总结下列表总结和比较了在上述的测定中使用的蛋白质。表43-45比较了A类、B类和C类蛋白质。表46-48比较了A类、B类和C类基因(细菌)。表中的任何数字可以用来作为定义本发明蛋白质和多核苷酸的上限和/或下限。表49比较了多种TC蛋白质的大小。另外，该表中的任何数字可以用来作为定义本发明蛋白质(和多核苷酸)的上限和下限。
这些表有助于表明一种令人惊奇的事实，即根据本发明甚至具有高度差异的蛋白质(在大约40-75％同一性范围内)能够被使用并且可以彼此替代。在此处TcdA2w-14以SEQ ID NO62重现，TcdA4w-14以SEQ ID NO63重现，并且TccCw-14以SEQ ID NO64重现。
注意tcdA3是个假基因(不编码全长蛋白质)所以不做该分析
注tcdA3是一个假基因(不编码全长蛋白)，因此不进行分析。

序列表<110>美国陶氏益农公司<120>混合并匹配TC蛋白质用于病虫害防治<130>DAS-104XC1<150>US 60/441,723<151>2003-01-21<160>64<170>PatentIn版本3.2<210>1<211>20<212>PRT<213>嗜线虫致病杆菌(Xenorhabdus nematophilus)<400>1Met Tyr Ser Thr Ala Val Leu Leu Asn Lys Ile Ser Pro Thr Arg Asp1 5 10 15Gly Gln Thr Met20<210>2<211>5<212>PRT<213>嗜线虫致病杆菌<400>2Met Trp Tyr Val Arg1 5<210>3<211>6
<212>PRT<213>嗜线虫致病杆菌<400>3Leu Thr Gln Phe Leu Arg1 5<210>4<211>10<212>PRT<213>嗜线虫致病杆菌<400>4Ala Asn Pro Gln Leu Ser Gly Ala Ile Arg1 5 10<210>5<211>8<212>PRT<213>嗜线虫致病杆菌<400>5Leu Leu Asp Gln Leu Ile Leu Arg1 5<210>6<211>39005<212>DNA<213>嗜线虫致病杆菌<400>6gatcaggtat tcaatcaacc caaactgttt gatgaacctt tctttgttga taatcgtact 60tttgattaca acgccattcg tggtaatgat gcacgaacaa ttaagcaact gtgcgccgga120ttgaaaatca ccgtagccac cttccaattg ttagctgagc aggtaaacac cgcctttcat180ctgccatccg gcaaattaac ctgttcactg cctgttattt cagcgcttta tcgtctggtg240
actgttcctc ggttatttaa tttaaccgct gaacagggca tgatgctgat taacgcatta 300aatgccagcg agaaattctc acctcatatt ctggctggtg agcctcgatt aagcctgtta 360acaacagagg gttcagatac cacagaggtc gatttattgg atgttattct gatgttggaa 420gaagttgctg tctggctgca acagagcaaa ctgaaaccgg aagaattctg cctgatgctg 480caaagtgtta tgttgccggt ggttgccacg gacagcagtg tgacattctt cgacaacctg 540ctgcaaggca ttcccaaaac cttactcaca gaagataact tcaacgcagg ggatatcccc 600agactccctg aaggagaaac ctggtttgac aaactttcga tgctgataac cagcgatgga 660ctcgtcaacg tttaccctct cagttggggc cagagtgatg aagattatct gaaatcagta 720ttgacacctg tcgtcgaaaa aatcattagc gatccaaaca gtgtgattat cactgtttcc 780gcattaacac aggtcattac tcaggcgaaa actgcgcagg aagatctggt ttccgccagc 840gtgacacggg aatacggtac tggacgtgat atcgttcctt ggttattacg ctggattggc 900agcagtgttc ccgatttcct tggcaaaatt tatatacaag gcgcaaccag aggcggacac 960ttgcgcactc cgccggatat cagcgctgaa ttactgcata tcacctatca tctggcgatg1020aataacatgc tgattaagca gttacgactc aaagctcaaa tcatttcatt acgtatcatc1080atgcctgaat ggctcggatt accaacgata gatggcagtc cgctatccgt gcatgaaatt1140tgggcactga gccggttccg taactgggcg accagctcat tgttcagtga agacgagtta1200atcgagtatt ttgcttttgc caatcagccg gagcaggacg ttcgtaacga tgaagatttt1260aatcgggact gtgctgaaaa gcttgccgac atactggaat gggatgccga tgaaattgag1320ctggcaaccc gacattttga tcctgcccca gcacgtgcca gaaatatggg acaaattgac1380tggctgcgtc gtgtcatggc gttgtcgcgt cagactggcc tgtcagtgac accgttaatg1440acagccgcaa cgttaccgcc tttcccgccc tatgaccaga taacccatgt cggtgaagcg1500gtgattgcgg caacccagta cccatcagag gagtaaggaa cgatgagttc agttacccaa1560
cctattgaag agcgtttact ggaatcacag cgcgacgcac tgctggattt ctatctcgga1620caggtcgttg cctattcacc tgacatgaca agtcagcgcg acaaaattaa ggatattgac1680gatgcctgcg actacctcct gctggatctg ctgacttccg ccaaagtcaa agcgacacga1740ctttcacttg cgaccaattc attgcagcaa tttgtgaacc gcgtgtcact gaatattgaa1800cccggtttgt ttatgaccgc ggaagagagc gaaaattggc aggaatttgc gaatcgttat1860aattactggt ctgcggatcg cttattacgg acttatccgg aaagctatct ggaacccctg1920ttacgcctga ataaaacaga attcttcttc caactggaaa gtgcccttaa tcagggaaaa1980attaccgaag attccgtaca acaagcggtg ctcggttatc tgaataattt tgaagatgtc2040agtaacctga aagttatcgc aggttatgaa gatggtgtta acatcaaacg cgataagttc2100ttctttgtcg gacgtacccg tacacagcca taccaatatt actggcgttc actgaatctt2160tcgatacgcc atcctgatac cgatgcgtta tctcccaatg cctggagcga gtggaaacct2220attgacctgc cattgggcag cgtagacccc aatttgatac gccccatttt cctgaataat2280cgcctgtata ttgcctggac ggaagttgaa gaacagtctg aaactaaaga tacaactgcg2340ttatcactgc ataaccaaaa cgttgagcct agtgcgggtg attgggttcc tcccacaccg2400ttcctgaccc ggatcaaaat cgcttatgcc aaatatgatg gcagctggag tacacccacc2460attctgcgcg aagacaatct gcaataccgg atggcccaga tggttgctgt gatggatata2520cagcaagacc cgcataaccc gtttctggct ctggttccgt ttgtccgtct tcaggggaca2580gataagaaag gtaaggatta tgattatgac gaagccttcg gttatgtctg cgatacactg2640ctggtagaaa ttactgattt gccggatgac gaatatgctg atggacgaaa aggaaaatat2700gtcggcaacc tggtctggta ttactcacgt gaacacaagg atgcagaagg caatcctatc2760gattaccgta ctatggtgct ctatccggca acccgggaag aacgctttcc tattgccgga2820gaagccaaac cggaaggaag ccctgatttt ggcaaagaca gtatcaaact gattgtcaat2880
tttgttcatg gcactgatga cacactggag attgtcgctc aatctgactt taagtttggt2940gcgatagaag atcatcaata ttacaacggt tctttccggc tgatgcacga taatactgtc3000ttggatgaac aaccactggt actgaacgaa aaagttcctg atttaaccta tccatcaatc3060aagctggggt cggataatcg aatcaccctg aaagccgaac ttctctttaa gcccaaaggt3120ggtgttggca atgaaagtgc cagctgtact caagagttca gaatcggtat gcacattcgc3180gaactgatta aactcaatga acaggatcag gtgcaattcc tttccttccc cgcagatgaa3240actggtaacg cgccacaaaa cattcgcctt aatacactgt ttgcaaaaaa actgatcgcc3300attgccagtc agggtatccc gcaggtactg agctggaata cacagcttat tactgaacaa3360cccatacccg gttcattccc tacgccgatt gatttaaatg gcgcaaatgg gatctatttc3420tgggaactgt ttttccatat gccatttctg gtcgcgtggc gactgaatat cgaacaacga3480ttaaaagagg ccaccgaatg gctgcactat atttttaatc cgctggaaga tgaacttgtt3540caggccagca accaaggtaa accgcgttac tggaattcac ggccaattat tgatcctcca3600cccaccgtgt accggatgtt aattgaacca accgatccgg atgccattgc agccagtgaa3660cccattcact accggaaagc aatattccgt ttctatgtca agaatctgtt agatcaggga3720gacatggaat accgtaagct gacatccagt gcacgtactg tcgccaagca gatctatgac3780tccgtcaata tgttactggg taccagccct gatattctgc tcgcggcaaa ctggcaaccc3840cgtacgctgc aagatgtggc tctgtatgaa aacagtgaag cacgggcaca ggagttaatg3900cttactgtca gcagcgtgcc acttctgcct gtgacatatg atacatccgt ctctgccgca3960ccgtctgatt tatttgtcaa acctgttgat acggaatatc tcaaactgtg gcaaatgttg4020gatcagcgtc tatataactt acgtcataac ctgaccttgg atggtaaaga gtttccggcc4080ggattatacg atgaacccat cagcccgcaa gatctgctca ggcagcgtta ccagcgtgtt4140gtggctaatc gtatggcggg catgaaacgc cgggcaatcc cgaattatcg tttcaccccg4200
atcatgagcc gggcaaaaga ggccgcagaa acgctgattc agtacggcag cacgttactg4260agtttgctgg agaaaaaaga caataccgat tttgaacact tccgtatgca gcagcaactg4320gggctgtaca gctttacccg caatctgcaa cagcaagcga ttgacatgca acaggcttca4380ttggatgcac tgaccatcag ccgacgggcc gctcaggagc gccagcaaca ctataaatcg4440ctctatgatg aaaacatctc catcaccgag caggaagtta tcgcattaca atcaagagcg4500gctgaaggtg tgatcgctgc ccagtcagcc gccactgcgg ccgctgtggc ggatatggtt4560cccaatattt tcggtctggc cgtcgggggg atggtctttg gcggtatgct tcgggcaatc4620ggtgaaggaa tacgcattga cgttgaaagt aaaaatgcca aagccaccag cctgagcgtg4680tcagaaaatt accgtcgccg tcagcaagaa tgggagctgc aatacaaaca ggcggatatc4740aacattgagg agatcgacgc acagattggt atccagcaac gccaactgaa tatcagcaca4800acccaactgg cacaattgga agcccagcat gagcaggatc aagtcctgct ggagtactat4860tcaaaccgtt ttaccaatga tgcgttatac atgtggatga tcagccaaat ctccgggctt4920tacctgcaag cctatgatgc ggttaattcc ctctgtttac tggccgaagc ctcctggcag4980tacgaaacag gtcagtatga tatgaatttc gtccaaagtg gtctctggaa tgatctttat5040caggggctgc tggtcggaga acatctgaaa ttagccttac aacggatgga tcaggcgtat5100ttgcaacata acaccagacg tctggagatc ataaaaacca tatcggtaaa atcattactg5160acatcatcac agtgggaaat tggcaagagt acgggttcat tcactttctt actgagcgcc5220gaaatgttct tgcgcgatta tccgacccac gctgatcggc gtataaaaac cgtagcgctg5280tcattgcccg cattgctggg gccttatgaa gatgtacggg cttcactggt acaactcagc5340aatacgcttt acagtactgc tgacttaaaa actatcgatt atttgcttaa ccccttggaa5400tacaccaaac ccgaaaacgt tttgctgaac gtacaggcta atcaaggtgt ggtgatttca5460acggccatgg aagacagcgg catgttcagg ctcaattttg atgatgaact tttcctgcct5520
tttgaaggga caggcgccat ttcacagtgg aagttggaat tcggttccga tcaggatcag5580ctgctggagt cgctgagcga tattatcctc catctgcgtt ataccgcgcg tgatgtgagt5640ggcggaagta atgagttcag ccagcaggtt cgtagccgtc tgaataaaca tcaattaaaa5700caagacaatt ctaactgata tcaggagccg gccccggaat ataacggggc cggaagtgaa5760attatgtctc aaaatgttta tcgataccct tcaattaaag cgatgtctga cgccagcagc5820gaagtaggcg catctctggt tgcctggcag aatcaatctg gtggtcaaac ctggtatgtc5880atttatgata gcgcggtttt taaaaacatc ggctgggttg aacgctggca tattcccgac5940cgcaatattt cacctgattt accggtttat gagaatgcct ggcaatatgt ccgtgaggcg6000acaccggaag aaattgccga tcacggtaac cccaatacgc ctgatgtacc gccgggagaa6060aaaaccgagg tattgcaata tgatgcactc acagaagaaa cctatcagaa ggtgggatat6120aaacctgacg gcagcggaac tcctttgagt tattcttcag cacgtgttgc caagtccctg6180tacaacgaat atgaagttga tccggaaaat acagaaccgc tgcctaaagt ctctgcctat6240attactgact ggtgccagta tgatgcgcgt ttgtcgccag aaacccagga taacactgcg6300ctgaccagcg acgatgcccc cggccgtggt tttgatctgg aaaaaatccc gcctaccgcc6360tacgaccgcc tgattttcag ttttatggcc gtcaacggtg ataaaggcaa gttatccgaa6420cggattaatg aggttgttga cgggtggaac cggcaagcag aagccagcag tggccagatt6480gcccctatta cattaggcca tattgtaccc gttgatcctt atggtgattt aggcaccaca6540cgcaatgtcg gtctggacgc ggatcagcgc cgtgatgcca gcccgaagaa tttcttgcaa6600tattacaatc aggatgcagc ctccggttta ctggggggat tgcgtaatct gaaagcgcga6660gcaaaacagg cagggcacaa gctggaactc gcattcagta tcggcggctg gagtatgtca6720gggtatttct ctgtgatggc caaagatcct gagcaacgtg ctacatttgt gagtagcatc6780gtcgacttct tccggcgttt tcccatgttt actgcggtgg atatcgactg ggaatacccc6840
ggcgccacag gtgaagaagg taatgaattc gacccggaac atgatggccc aaactatgtt6900ttgttagtga aagagctgcg tgaagcactg aacatcgcct ttggaacccg ggcccgtaaa6960gaaatcacga tagcctgtag cgccgtcgtt gccaaaatgg agaagtccag cttcaaagaa7020atcgcacctt atttagacaa tatctttgtg atgacctacg acttctttgg taccggttgg7080gcagaataca tcggtcacca tactaacctg tatcccccca gatatgaata tgacggcgat7140aaccctcctc cgcccaatcc tgatcgggac atggattact cggctgatga ggcgatccgc7200tttttactgt cacaaggtgt acaaccggag aaaattcacc tcggatttgc taactatgga7260cgttcatgtc tgggtgctga tctgacaact cgccgctata acagaacagg agagccactg7320ggcacgatgg aaaaaggtgc tccggaattc ttctgtctgc tgaataacca atacgatgcg7380gaatatgaaa ttgcacgcgg gaaaaatcag tttgaactgg tgacagacac ggaaaccgac7440gctgacgcac tctttaatgc tgacggtggt cactggattt cactggatac gccccgcact7500gtgctgcata agggaattta tgcaaccaaa atgaaattgg gcgggatctt ctcttggtca7560ggcgatcagg atgatggcct gttggcaaat gctgctcacg aaggtttggg ttacttacct7620gtacgcggaa aagagaagat tgatatggga ccgttatata acaaaggacg tctcattcag7680cttcctaaag taacccgtcg taaatcgtag taaataaaat tttccggtgg cctcacaggg7740gtcaccatat cctgctgtga aaaagcgtat ccatttaatg ctttaacgct tcaattttct7800cccggctcag gccggtactg gtgacaatga tgtccagact gacaccatgc cgtaataatg7860cgcgcgccgt ttccagcttg ccttcttccc gtccttcagc tctgccttct gttctgcctt7920cagccctgcc ttctgtccgg ccttgctcac gccctttttg ttcaagctgt tctgcaatag7980tcatcaacat ggtttcatgc tccggagatt gttcagtcag ttgatggaca aactgggcga8040gatccagcgt atgtccattc agtaaaatat agcttaacac aacatggcgc tgttcggcgc8100tattataacc ggcattcaac aacgccacta attggggaac ccactccagc atatcccggc8160
aacggatatg tttttgtacc agctccatca aggcaatgct tttatgtgtc aggatctctt8220catcactgag cgcactgata tccaccaacg gcaggggctg attatacagg tgagccgcgt8280gttcagagag tgtaaaacaa tccagccatc gatttgagta agggtaaggc ctcacctcac8340catgataaaa cagcaggggg acgaccaaag ggagttcagt atgtcctttt ttcagatgcg8400cagccatggc tgacagcgaa taatacatca gccgccaggc cattaacgga tcaggcgtgg8460actggtgttc aatcaggcaa taaatgtaac cgtccccgtg ggttgtctcg acagaataca8520gcacatcact gtgcaactga cgtaattgcc tgtccacaaa gctgccgggt tccagtttta8580gtgtggttaa atcacacact gaccggatcg cttccggcag ataaagggat aaaaattccc8640gggcggtttc tggttgggtt aaaaaatgtt tgaataacgc gtcatggtga ggcttttttg8700ctttcctggc cacaatccgt ctctctgttt tatcggttat taatcgcctt tactgccaaa8760gctatcatct cgctgaaaaa tccacagcca atatacaaca tattatctgc tgacccaaca8820ctcgtccggc taatcaatcc agtatcaatg cgagttctac agtaaataca gctcttcatg8880gtcaggaaac cggacaaaag ttgattgaat ttcctaacca tgaattttct gttatgttaa8940ttattaccgt ctcacaataa taatcacatc caacagaatt tatttactat ataaataaac9000tatcaattat tataagaaaa ataatatgat tggcattaaa tataaaacca taaaaaagta9060gaattaattt ttaaaactta attgcagaaa ccagatgaaa tataaactta atttcttatc9120cataaataat aatgaatcaa tatttattca ataccatcag tggaaggttc ccgtttgttt9180taatttcaag cttataatcc cctttgcctt tagctgaatc accagacata atttgcttat9240tgctaaattg tttactactg tctgtaaaat aaacataact gccatgttga aacatgtagt9300tcacaatatc agcagcgtcc tttttactga aagtaacttt gatataatgg ccagagttaa9360tatctttctg actatcgcac caaggaatcc acataccacc ggtagatgaa tcatttcccg9420gagaaacaac cacatggtca ggtattatgg ggaataactc atttgctgac tcctgattaa9480
ataaatccgc tttatattca caaccaaaat tgttatcaac attaataata ttacgaacat 9540ctgacataat aatttccccc gaatatagtt taaaggtttt ttcaatttaa taacatatca 9600aaggaactat aatactgtat atttacatcc gtcaacatta ttcacctaca gggtgacatt 9660cctctattaa ataaaaaata agttttgatt tttaactttt gataacttat gcaccaaatc 9720agtgaccact gccgttaact tagttttgat cctcgtcact acggttaaac ttccgactcc 9780cagaaagcaa aaaaccccgc gagtgcgggg ctatattcaa agtgcttgag ttatttcact 9840atgcggatag ttttgacatc aatttcaaca ctgttccagt ctttgtccac ttcaccttcg 9900atacgaactt tgtcagttgg agtggccgtc agacccatcc agcgcttatc atcaatgtca 9960acataaacag aaccactgtt atccctgaat tcatagagtt cgtgaccaac ctgtttaaca 10020atgtttcctt ccagaacaac ccacgcatca tcacgaaaag attttgcttg agcaacgctg 10080gtcaggttgg gagttggacc tttaaatcca ccctgagtat agtctgtgct gtctggggaa 10140acgaagccac cctgctgtgc caaagcacca aaagaaaggg tactgagaat aagagtaatc 10200agtgtttttt tcatagcttt ctctttgatt atgcgaagaa aaaccccgca tttgcgaggt 10260tcgggtattc aataaattat gtgacattac tatcactctt gtcacgatat atcaactttt 10320gtaattacgc aactttatta aggatttctt tttgcacaca tttatctgac tccaacgtag 10380ccccctgaaa ccagcaagac atcctcaata aataatcttt catagataaa tattagttat 10440tcatttttca aacagcacaa acacaattaa aaatatttaa acaattgttg agttgaattt 10500tttcatgaaa gtttgttaaa atttaatttt taacatacgg tattcattat ttaaatccat 10560gtattatagg gaagttcttt attttttatt gaaagaatag agcgataaat cagtatcaat 10620ttaattaacc ataatattcc tatcagatta taataatctc cacctaaaaa ccattaatca 10680ttaaattgac aataacttaa ggatttatat gataaaagtt aatgaactgt tagataagat 10740aaatagaaaa aggtctggtg atactttatt attgacaaac atttcgttta tgtctttcag 10800
cgaatttcgt cataggacaa gtggaactct gacgtggcga gaaacagact ttttatatca 10860acaggctcat caggaatcaa aacagaataa acttgaagaa ctgcgcattt tgtcccgtgc 10920taatccacaa ctggctaata ccactaacct taatattaca ccgtcaaccc taaacaatag 10980ttacaacagt tggttttatg gccgtgccca ccgttttgta aaaccgggat caattgcttc 11040catattttca ccagcggctt atttaacaga attatatcgg gaagcgaaag attttcatcc 11100tgacaattct caatatcacc tgaataaacg acgccccgac attgcttcac tggcactgac 11160acagaataat atggatgaag aaatttccac attatcctta tctaatgaat tactgctgca 11220taatattcag acgttagaga aaactgacta taacggtgta atgaaaatgt tgtccactta 11280ccggcaaacc ggcatgacac cctatcatct gccgtatgag tcagcccgtc aggcaatttt 11340attgcaagat aaaaacctca ccgcatttag ccgtaataca gacgtagcgg aattaatgga 11400cccaacatcg ctactggcta ttaagactga tatatcgcct gaattgtatc aaatccttgt 11460agaagaaatt acaccggaaa attcaacaga actgatgaag aaaaatttcg gtacagatga 11520tgtactgatt tttaagagtt atgcttcttt ggctcgctac tacgatttgt cttatgatga 11580actcagttta tttgtcaatc tctccttcgg taagaaaaat acaaatcaac agtataagaa 11640tgagcaactg ataacattgg tcaatgacgg gaatgatacg gcaacggcaa gattgattaa 11700gcgaacccgc aaagatttct acgattcaca tttaaactat gcagaactaa ttccaatcaa 11760agaaaatgaa tacaaatata atttcagtgt aaaaaaaaca gaacctgacc acttggattt 11820tcgtctccag aatggagata aagaatatat ataccaagat aaaaatttcg tccccattgc 11880taatacccat tacagtattc ccattaaatt gacgacagag caaatcacca acggtataac 11940actccgctta tggcgagtta aaccaaatcc gtcggatgct atcaatgcca atgcatactt 12000taaaatgatg gagttccccg gtgatatatt cctgttaaag ctgaataaag cgattcgttt 12060gtataaagcc acaggcatat ctccagaaga tatctggcaa gtaatagaaa gtatttatga 12120
tgacttaacc attgacagca atgtgttggg taagctgttt tatgttcaat attatatgca 12180gcactataat attagcgtca gcgatgcgct ggtattgtgt cattcagata tcagccaata 12240ttccactaaa caacaaccca gtcattttac aatactgttc aatacaccgc tattaaatgg 12300ccaagagttt tctgctgata ataccaaact ggatttaacc cccggtgaat caaaaaacca 12360tttttatttg ggaataatga aacgtgcttt cagagtgaat gatactgaac tgtatacatt 12420atggaagctg gctaatggcg gaacaaatcc agaatttatg tgttccatcg agaacctgtc 12480tctgctttat cgcgttcgtc tgctggcaga cattcatcat ctgacagtga atgaattatc 12540catgttgttg tcggtttctc cctatgtgaa cacgaaaatt gccctttttt ctgatacagc 12600attaacgcaa ttaatcagct ttctgttcca atgcacccag tggctgacaa cacagaaatg 12660gtctgtcagt gatgtgtttc tgatgaccac ggataattac agcactgtcc ttacgccgga 12720tattgaaaac cttatcacga cactaagtaa tggattatca acactttcac tcggtgatga 12780cgaactgatc cgtgcagctg ccccgctgat tgctgccagc attcaaatgg attcagccaa 12840gacagcagaa actattttgc tgtggattaa tcagataaaa ccacaaggac tgacattcga 12900tgatttcatg attattgcgg ctaaccgtga tcgctcagag aatgaaacca gcaacatggt 12960ggctttttgt caggtactgg ggcaactttc tctgattgtg cgcaatattg gactcagcga 13020aaacgaactg accctgttgg tgacaaaacc ggagaaattc caatcagaaa ccacagcact 13080gcaacatgat ctccccactt tgcaagcgct gacccgcttc catgctgtga tcatgcgttg 13140tggaagctac gcgacagaaa tcttaacagc attggaacta ggagcgctga ctgccgaaca 13200attggcggtg gcgttaaaat ttgatgctca ggttgtgaca caagcattgc aacagaccgg 13260tttgggagtg aataccttta ccaactggag aactatagat gtcactctgc aatggctgga 13320tgtcgctgct acattgggta ttaccccgga tggtgttgct gcactcataa aattaaaata 13380tatcggtgaa ccagaaaccc cgatgccaac atttgatgat tggcaagccg ccagtacttt 13440
gttgcaggcg ggactgaaca gtcaacaatc cgaccagctt caggcatggc tggatgaagc 13500cacgacgaca gcggccagtg cttactacat caaaaatagt gcacctcaac agattaagag 13560ccgggatgag ttgtacagct atctgctgat tgataaccaa gtttctgccc aagtgaaaac 13620cacccgtgtg gcagaagcca ttgccagcat tcagttatat gtcaaccggg cgttgaataa 13680tgttgaagga aaagtatcaa agccagtgaa aacccgtcag ttcttctgcg actgggaaac 13740ctacaatcga cggtatagca cctgggccgg cgtatctgaa ctggcctatt atccggaaaa 13800ctatatcgac cccacgattc gtattggtca gacaggtatg atgaacaacc tgttacagca 13860actttcccaa agtcagttaa atatcgatac cgttgaagat agctttaaaa attatctgac 13920cgcatttgaa gatgtcgcta acttgcaggt gattagcgga tatcatgaca gtatcaatgt 13980caatgaggga ctcacttatt taattggtta tagccagaca gaacccagaa tatattattg 14040gcgcaatgtc gatcaccaaa agtgccagca cggtcaattt gctgccaatg cctggggaga 14100atggaaaaaa attgaaatac ccatcaatgt atggcaggaa aatatcagac ctgttattta 14160caagtctcgt ttgtatttac tgtggctgga acaaaaagag ctgaaaaatg aaagtgaaga 14220tggcaagata gatatcactg attatatatt aaaactgtca catattcgtt atgatggcag 14280ctggagctca ccgtttaatt ttaatgtgac tgataaaata gaaaacctga tcaataaaaa 14340agccagcatt ggtatgtatt gttcttctga ttatgaaaaa gacgtcatta ttgtttattt 14400ccatgagaaa aaagacaatt attcttttaa tagtcttcct gcaagagaag ggatgaccat 14460taaccctgat atgacattat ccattctcac agaaaatgat ttagacgcca ttgttaagag 14520cacattatca gaacttgata ccaggacaga atacaaagtc aacaatcaat ttgctacaga 14580ttatttggcc gaatataagg aatctataac cacaaaaaat aaattagcca gttttaccgg 14640aaatattttt gatctctcgt atatatcacc aggaaatggt catattaatt taacgttcaa 14700tccttcaatg gaaattaatt tttcaaaagg caatatatat aatgatgagg ttaaatacct 14760
gttatcgatg gtagaagatg aaacggttat tttatttgat tatgatagac atgatgaaat 14820gcttggaaaa gaagaagaag tttttcatta tggaactttg gattttatta tttccatcga 14880tcttaaaaat gccgaatatt ttagagtgtt aatgcatcta agaaccaagg aaaaaattcc 14940tagaaaatca gaaattggag ttggtataaa ttatgattat gaatcaaatg atgctgaatt 15000caaacttgat actaacatag tattagattg gaaagataac acaggagtat ggcatactat 15060atgtgaatca tttactaatg atgtttcaat cattaataac atgggaaata ttgcggcact 15120gttccttcgc gaggatccat gtgtgtattt atgttcaata gccacagata taaaaattgc 15180ttcatctatg atcgaacaga tccaagataa aaacattagt tttttattaa aaaatggctc 15240tgatattcta gtggagttaa atgctgaaga ccatgtggca tctaaacctt cacacgaatc 15300tgaccctatg gtatatgatt ttaatcaagt aaaagttgat attgaaggct atgatattcc 15360tctggtgagc gagtttatta ttaagcaacc cgacggcggt tataacgata ttgttattga 15420atcgccaatt catataaaac taaaatccaa agatacaagt aacgttatat cactgcataa 15480aatgccatca ggcacacaat atatgcagat tggcccttac agaacccggt taaatacttt 15540attttccaga aaattagctg aaagagccaa tattggtatt gataatgttt taagtatgga 15600aacgcaaaat ttaccagagc cgcaattagg tgaagggttt tatgcgacat ttaagttgcc 15660cccctacaat aaagaggagc atggtgatga acgttggttt aagatccata ttgggaatat 15720tgatggcaat tctgccagac aaccttatta cgaaggaatg ttatctgata ttgaaaccac 15780agtaacgctc tttgttccct atgctaaagg atattacata cgtgaaggtg tcagattagg 15840ggttgggtac aaaaaaatta tctatgacaa atcctgggaa tctgctttct tttattttga 15900tgagacgaaa aatcaattta tattcattaa tgatgccgat catgattcgg gaatgacaca 15960acaggggata gtaaaaaata tcaaaaaata taaagggttt attcatgtcg ttgtcatgaa 16020aaataacact gaacccatgg atttcaacgg cgccaatgca atctatttct gggaattgtt 16080
ctattacacg cccatgatgg tattccagcg cttattgcaa gagcagaatt ttaccgaatc 16140gacacgctgg ctgcgctata tctggaaccc ggccggatat tcggttcagg gtgaaatgca 16200ggattattac tggaacgtcc gcccattgga ggaagatacg tcctggaatg ccaatccgct 16260ggattcggtc gatcctgacg ccgttgccca gcatgatccg atgcactata aagtggctac 16320ctttatgaaa atgctggatt tgttgattac ccgcggagat agcgcctatc gccagcttga 16380acgtgatacc ttaaacgaag ctaaaatgtg gtatgtacag gcgctcactt tattgggtga 16440tgagccttat ttttcattgg ataacgattg gtcagagcca cggctggaag aagctgccag 16500ccaaacaatg cggcatcatt atcaacataa aatgctgcaa ctgcgtcagc gcgctgcatt 16560acccacgaaa cgtacggcaa attcgttaac cgcattgttc ctccctcaaa ttaataaaaa 16620actgcaaggt tactggcaga cattgacgca acgcctctat aacttacgcc ataacctgac 16680aatcgacggt cagccactgt cattatctct ctatgccacg cccgcagatc cgtccatgtt 16740actcagtgct gccatcactg cttcacaagg cggcggcgat ttacctcatg cagtgatgcc 16800gatgtaccgt tttccggtga ttctggaaaa tgccaagtgg ggggtaagcc agttgataca 16860atttggcaat accctgctca gcattactga acggcaggat gcagaagcct tggctgaaat 16920actgcaaact caaggcagtg agttagccct gcaaagtatt aaaatgcagg ataaggtcat 16980ggctgaaatt gatgctgata aattggcgct tcaagaaagc cgtcatggtg cacagtctcg 17040ttttgacagt ttcaatacgc tgtacgacga agatgttaac gctggtgaaa aacaagcgat 17100ggatctttac ctctcttcat cggtcttgag caccagcggc acagccctgc atatggccgc 17160cgccgcggca gatctcgtcc ccaatattta cggttttgct gtgggaggtt cccgttttgg 17220ggcgcttttc aatgccagtg cgattggtat cgaaatttct gcgtcagcaa cacgtattgc 17280cgcagacaaa atcagccaat cagaaatata ccgtcgccgt cggcaagagt gggaaattca 17340gcgcaataat gcggaagctg agataaaaca aattgatgct caattagcga cgctggctgt 17400
acgtcgtgaa gcggcagtat tacaaaaaaa ctatctggaa actcagcagg cacaaactca 17460ggcgcagtta gcctttctgc aaagtaaatt cagtaatgca gcgctataca actggctccg 17520tggaaggttg tccgctattt attatcagtt ttatgatttg gcggtctcac tctgtttaat 17580ggcagagcaa acttatcagt atgaattgaa taatgcggca gcacacttta ttaaaccagg 17640tgcctggcat gggacttatg cgggtttatt agcgggtgaa accctgatgc tgaatttagc 17700acagatggaa aaaagctatt tggaaaaaga tgaacgggca ctggaggtca ccagaaccgt 17760ttctctggct gaagtgtatg ctggtctgac agaaaatagt ttcattttaa aagataaagt 17820gactgagtta gtcaatgcag gtgaaggcag tgcaggcaca acgcttaacg gtttgaacgt 17880cgaagggaca caactgcaag ccagcctcaa attatcggat ctgaatattg ctaccgatta 17940tcctgacggt ttaggtaata cacgccgtat caaacaaatc agtgtgacat tacctgccct 18000tttagggcct tatcaggatg ttcgggcaat actaagttat ggcggcagca caatgatgcc 18060acgtggctgc aaagcgattg cgatctcaca tggcatgaat gacagtggtc aattccagat 18120ggatttcaat gatgccaagt acctgccatt tgaagggctt cctgtggccg atacaggcac 18180attaaccctc agttttcccg gtatcagtgg taaacagaaa agcttattgc tcagcctgag 18240cgatatcatt ctgcatatcc gttacaccat tcgttcttga tccaaaaatt aactggacag 18300agaccctgta cgggtctctg tccacacatc cgaaaaaccc accttgtcat ccatgacaaa 18360gtgggaatga acatgattgt tatgcttcgg attcattatg acgtgcagag gcgttaaaga 18420agaagttatt aaaagcccgc ttaaagccgc tccaggtaac ccggctagcg gcattggcaa 18480cttcccctcc aacggcatga tgagcggccg cggctgtccc gccaatggct gcaccaaccc 18540attcaccggg tgtacggcta taaggtaata atacttcaga aatatttctc ccgacacttt 18600ctcctatcat tcggccaaac cagctcctgg aactgacagc gtgggaaatg gcagagctaa 18660tgcctcttct gagcagtaac ctgccgataa accgataagg gccatcccat agattaccaa 18720
tgatccttcc ccatcgagca ccatacatag caccaatcgc tgcccgttca cccagctcag 18780aacttccctg atggcggcca agtaatatgc cgccaataat tgcgcctgat agtgccccta 18840accgctctgg cgcgctgaca ttaccgggcc tgagcgtatc cagcgtacct tgtccggcgg 18900gtgtggcaat actgatagcc atgcccgtgt tatgctctcc ggctaaagcc attaatcctc 18960caacggtgac cgctgttgct gcggaaatgg cggtacctgt cgaagagctg ttaaatagtg 19020cagacgtcac aagcgatgtg acaacaaaag cgccaacctg aacaggaaca gaacgtttac 19080gcgtcagata acttaaaact tccccaattt tttctgagat gttgttcgcg aaaaacccca 19140tcaccgcccc ggagacaaaa ccaccaatgg cagccccgac aatcccccaa ggcgacgctc 19200ctgcaatcgt ggccgccttc acccccagac ttgctacccc cacacccaaa acaaacgttc 19260gcaatcctcg gtttaatttc aagaacgtat caaaggaagc gccttgttca agcaggtgtt 19320ctgtcgtgat gttgactgcc tttcgatacg cttttttccc tatccaggca aggacaccct 19380gaccggggaa acgaccatca gaatcagaaa aaacgatggg gttattcctg cacattcgga 19440acaaattgag accatcgacc tcaccggcag gatctacact caaccatcgc cctgtccacg 19500attgataata acgataaccg tagtaataca accctgttgc atcccgctct ttgccagaat 19560aacgcacggt tttgtaatca gcttctgact gacttcgggc tgcccacacg gcggttcccc 19620cataggggta atattcttcc tgactaatga tctgcccgtc actgtccaat tccagcccgc 19680tactgccaat caggttgcca taactgtagc gcagctgatc attgctgata tccgccggtt 19740tgcctgtttc ccaatgcagc acccgcactt gtgcctgacc cgattcaccg acagtgatga 19800cctgcaaaaa ctcttttaat gtattgccgc tatatgtcgt gcgccattcc agctctggca 19860aatataatgt tcgctgtatt tgctcactgt tacctgtctt ctgaatatga gtcttaatga 19920cacgctgact gtctgcatca taacggtaga attcctgatc aggcgtcgta ttttccctat 19980tgaccaatat cacttgttgc aattcgtcac ggggtgtcca gaaaagatcc tgaccgggaa 20040
caagccgggt ctgatgcccg ccgggggtga acaacatatc cacctgagtg ggatcttgcg 20100ccagctcttc cagtacagcc cggttgctgt gatctgaaac ggtcatgttc gttgtatagt 20160tattaccggt gatcggtgaa ttatggcgaa ttctggtcag atttccccca cgatcatagt 20220cgtaagtgcg agagtaattc gtataagtat tgttatcaat cagagcgggg atgggtaact 20280ggtttttttg tcggccaata ttcgccattt cacgcccagt gacggaaacc agctggtaca 20340ggctgtcata ggtgtaagta ttttccggta caattttctg gttgcgccaa aagcgggtaa 20400tttcagcatc attagttgat ttcagcacat ttccgacagg atcatattca taacgcaggt 20460tttgtaaaat tttctcccca gcggcatgac cggaaggacg ttctgttttt atgccaataa 20520ctcgttgcgt ctcgggttca taggtatatg tagtcactat cccgttacca tgttcctccc 20580gtagcttctg gctggcagcc gaataggtca gggatttcac gataacttgt tcttgtttcc 20640ccttcagcgc caaccaactg ccttgaagca gaccggccac atcataggcg atacgttgct 20700tgtttccggc agcatctgta ctcgttaata ccgtgccggt agcatccgtt gtgctgacag 20760aagtgaagct ttccggcgcc agcgcgtttt tccagccaga ttcatccata ccgtgccaat 20820cggcttcgct gtcatctttc agtaattgct gtgtgatgga caagggtatg ctggttaacg 20880atatgctgtt ggtttgattc attccggtgg gatcataatg gaccacgcac tggccggcca 20940gattattgcc tttttctgcc ggcgtatttc ctgaccagat caatcgctcc gtgatacagg 21000cgttctctcc ttttacctgc tcggtaatcg ttagcaatcg tcccggaagg ttatcacttt 21060catactgaaa cgttcggcta acgccattgg cgctgacagc taaaacggga cgcccggcaa 21120catcatgcag ggcgacacgg gttccggcat ccacactttg cgtacgcaat gccttcttac 21180tgagtgatga caagagaata agattgggtg taatggcgtt cttgtcactc gctgtctgct 21240ggcgttcata aaatcgcgga tcaatactct gagtcagaga tccttgagca tcatattgat 21300aaccggtgat gcgttcatcg gttacctgag gtgtatcggg gtgccgatac caggctattt 21360
cgcgtactgt ctgaccacgg ttgtccagta cggtgacgga tggcgtattg ctgtgaacga 21420aattcttcat gattcattcc taaatggagt gatgtctgtt cagtgaacag gcatcactga 21480gctttatgct gtcatttcac cggcagtgtc attttcatct tcattcacca caaaccaggg 21540agtgaataag gatcgacgaa acccgccttt ggccgtgata acctgatatt cacgccccaa 21600cggatcatag taatgggtat cggcatatat atcctgccgg gcactgtcat cactgacgta 21660ctgccaacta ttcaggaaat acggttgata cttacgcagg gcttggcctt ttccgtcata 21720ttctgtacgt ccggaaactg cccaacggaa atctgtcatc gccgtttcag gcgcgccatg 21780attttcagcc acaatggctc catactcatc acgtacccag gcttcaccac tttcatggcg 21840tacggctgtt tgtaaggttc gcccaaaacc atcactaaac gtaaacgttt gacgtaattg 21900ttgttccgga tcggcatcat agcggtcggt gatcacactc agtacatggg gtgggttctg 21960tgaattgact tgctttggca tggcagcggc agggttattt tgttgccagc ggcgaaaagc 22020aagcgacagg agataaccat cttcagtgat gatcccagcc ggtttcagct ctccataaag 22080ctccccatca ttagaaaagc tggcctgaac catccagctc agaggggcat aaaccatcag 22140ccctgcaaca ggtataccgg gtttcaatgc cagagcatca tccaccgttg tggggacaat 22200aaaggggaca gtttcatttt ccgcaggggt atatccttgt ttttcaccgt tttcagtccc 22260ccagaaacgg aagctggtta ccctccccag tgcatcaaac gtcacggtgt gatagttatc 22320attgacatct gtggtgttat ccgcaaccat aaatcgataa tcgtaatgcg cttgcatacg 22380caggccagcc gcatcctctg ttgcggtgat aacacagtaa tggctatccc acgtgactgt 22440cgttttacct gtaagcttgg tttcccgttg caccaatggc cgatagaatc cgtctgcacc 22500ggcatattct gtaaattcct tttgtcccac ccagacatgg aaatctgtct tttcactgaa 22560cggcactttt gccgtattcc agcccgcatc attcagctgt tttgtcagct cctgctcatc 22620catcacctcc tcaaaagccg ccaacgatcg ttcatcaaac tctgcggttt caatgtatgc 22680
caccagcgga ggaatagcgg gttgttcttc tggaccggta tatgctacac gctgatgtcc 22740cagataatcg gctgcggcat caggcaacaa caatgctcct gcacctgtgg cagaaaacca 22800ttcaagggaa aatccaccgt ccggcacttt atcggcttga taaatacgtg cgtcactgcg 22860tgaggtatcc ataagccctg tgatccacgt attatcatca tgattcagat gatgataaga 22920agaacgctgg cgtgtcagac gaaggaacat ctgctgttcg tcgaaactgc tggtgaaaag 22980tgtttcgggc agggtatccg gataaggcga gaactcaggc tgtggacgtc tcgaataggc 23040aatctcaaga ttgtcctgcg gaaatcctaa cgcatcagat ttaaggacga tcttttggct 23100gcactgtgga tcggtagcaa cccgttcata tcggtattgg cgggattcgg ccaccgaaac 23160cagtaccgca ggcacgtccg ataccatcac cggtaacaaa cgtacttggg tgcgggattc 23220atccactgaa taaggcgtac cggccagtat agaatcatca tccccataca gctcactgcg 23280taaacgttgt ccttttaagg ctcgatgtaa ccagtattct tcctgttcgc tcggcgtgac 23340cgtcatatca ccaccggatt tttcgtcata acgggtaaag cgtggggtaa aatggggaaa 23400tgcctgttga tccccctgcc aatattccgt gggcagaaga atatcgactt cccgtacgcc 23460agtgccgtac caattaaccg tgcgcgaagg tgccggtggt tcagcatgtg tcccctgtgt 23520cgcactcgcc cgtgaatcaa tatcagtttg tgtcacccgc ccaaaaccac gaaactcccg 23580ttccagacca tcccaggcac catgtgagta atgataatgg ctggtcaatc ggttaccgga 23640aatttcatcc agcacttccg tgcgccacaa cacatgcacc gggaacggta agtagctgac 23700caccgtcatc ccggattcag aagcctgtaa tttctcatcc agccagaact gggcagagct 23760gcgataatac agcgtggttt ctgttcccat attgttattg acggcattca gcagccaagg 23820cttgaatatg gtcatatcca atcgccagtg ctgcaccttc atatggggga tcgtcaaaat 23880aatgctggca gtccctaatc cttgtgtatc cgctatttgt aaccgacaag tatcatcaaa 23940acgtacccca tccggcagat caatacgctg aggttcagca aaatgattgc cgctttcatt 24000
ggcatagagt tcaaggtaag tattgcgggc ataaataaaa tcggtggtgc ctgagccatc 24060tatgtctacc atatacagtc tgtcggggtt aaacgtttcc ccgctaatct ggaagcctgt 24120catcatcaga ggctcaccaa attttccatg ccccaggttc ggccagtagc gcacgctatc 24180tgccgttact tccaccagat gtgattgccc ggagcctgtc atatcactga atgcgacaag 24240atgacgctca tttctgccgg gaaccggcag tggcatatct gacaaatgaa tcacatcctg 24300agcgcgatcc catcctgccc gattatttga ccagacacgt acactatttg gcccgataag 24360cgctaagtca ggcagcccag ccccatcaat atcagccagt tttgcctgcg gatggaaata 24420ttccattggc acagcggata atggaataaa gggtgtccat tcaccttccg gtgacatggt 24480gtggtagccc cgtaaccctg atgccgtaat cacccaatcc agacgcccgt caccattgat 24540gtccaacaac atcgcgcttt cctgttgtgc cggaatatgt ggcagtggtt tggcctcctc 24600ataggtaacc gcattcgttc cttcggcagt gatatcccgt accggagcac ggtaccacca 24660ggctttctga gtatcctgat aaagtacgcc ggaaattcct tctccatata aatcaaccaa 24720ttggtatggc tgcaacgtgt tcattttttc taactgcggc atggactgcc agttcagatt 24780cacgccatga ttaacacgtt gataatccat ttccagcggg gacatcatca ctggcgtacc 24840gtccgtttca tgggccagtc tgcgggccgt ttgcagcaag gaaaccttgt tgttcaggtc 24900ataatccaga ataagacggg aaaccagcgc cggtgtttct tctgcaacct tttcccctgc 24960cagcgctttc agctgatgaa acatcagaac ttggcgacac aagcgacggg ttcgaatttc 25020aaacccatat tcatagcggg agaaactgtc cggacgacaa cgccattttt caggcacatt 25080gttttcagac acattgtttt ctgacacatt gaattcgggt acagagttca gcgaagatga 25140gcgctcaccg taatcaaata ccagatgaaa cagccagtca ttatcagcag gaatacctga 25200ttttaccgcg aaaaaagcgg tttccggctg agtattgcca tagctgactt ttgccagata 25260acgctgggcc gtaacacctg aatgctgagc aagttcatgc tcatcacagt caagatcgtc 25320
ttctgcccga tagtgatagt aaatatgttc cccggtatgc gtgacggttt cctccatcag 25380ccagcgggca attctggttt catcctgcgg gtcagcaata cgtgcatggt gatgcttacc 25440gaataggtgc actaaaccat ccgcagtaaa aagtacccaa aaagacgtct cttcctcacg 25500tctctgctgt ggctgccagt gttctaaacg aacgattttt tctgccacgc gggactgata 25560gcgggtaaca gtatgcggct gtgtcagaac cgtccccaac agtgaggttg cggtgcgttg 25620ctctggttgc ccttggctgt ccggcacaat actcaacact tccccatccg gcccgagata 25680ctcatcttgt cccgtatagt gcggaacgcc cttggcggta cgcaggctga taaaaccaac 25740cccacattgc caccccatcc cgaatgaccc attgccggca gtactgctgt aattcagtga 25800tagcaccggc accagaccac gcccgacaga gatcggcaag ggcagtgaaa atgacgctcc 25860cccttccgct ccgacggcat tgagtgcttc tcccattcct tttagtgatc cgcccccaga 25920gggcaatgac ggtatttcaa gtttcaaagg tgttgaaccc tgcataaaaa ctccttaaac 25980aggctccctc aggagcctgc ctatcacaat gttttaatta agaacgaatg gtatagcgga 26040tatgcagaat gatatcgctc aggctctcca gcagcgcttt ctgccgatca gtcgcatccg 26100ggaaactcaa cgtcaggctg ccgctgtcat tcacggaaat accttcaaac ggcagataac 26160gggaatcgtt gaaatccagc ataaattgac cactgtcatt cacgccgtgg gagagagcaa 26220tagcactgca accgcgtggc atgacgatgc tgcccccgta attcagcacc gcccgaatat 26280cttcatacgg cccaaccagc gccggcaagg tgacactcac ctgtttcaac tgacgggtat 26340tgccaaggct ttcggggtag tcgctgaaaa ttttcaaatc agacaatcgc actgaggctt 26400ctatctgacg gttactgagt tttaattcat tgccggaagc tcctacgttg cctttccctt 26460cacgcaggaa ttgcgtgagt ttttcggtca gattaaagtt gtctgatgat aaggcctgat 26520agaactgtgc caacgagacg gtacgggtca cttccagtgc ccgctcatca cgctccagcc 26580agactttttc catttctgcc agattcagca gcaacgtttc acccgccatc aaacccgcag 26640
tcgtaccgtt ccaggcccca ccccggataa aggtaacacc gttgtcggtc agctcgcggc 26700gcagcgcttc ctgtgccatc aggcagaagg actgggtcag gtcaaagaac tggtaataga 26760tagcactcag cttgccgcgc atccaactgt aaagcgcttt gtttgtgaat ttacgctgta 26820acagctctaa ctgagcctga gtatgggcct gctgggtctc ctgatattcc acctgcatct 26880gtgctgcttc gcggcggatt ttcaggcttt ccaactgggc atccatttgt ttgacttcac 26940cgtcagcatt atcacgctga atttcccact cctgacggcg gcggcggtag gcttccgaac 27000ggctgatttt gtctgcggaa tattgggaag ctgtggcaga aagcgacatc acggaggcgg 27060aagcacgcag tgctgccccc caacgactgc cgccacaagc taaaccgaac acgtttggca 27120ctaaatcggc caccccttcc gctattgaaa gcacctgccc ggccagagac tgacctgccg 27180ctgcatcaag cagtgacatt gcccgctgtt ctccgtggtt gatatcctcg tcatacagct 27240gctggtattt ttccagacga ttttgtgcac tgcggcggct ctctgccaat acagcaatat 27300cagcatccac ttcatcgaca gttcgttgct gaatacggat gctctgtgtc gccagttcca 27360taccctgctg tagtagcagc gtggtgagtt catcggcatc atcatgctct gccatactga 27420gcagagaggt gccgaactgg gttaattgcg ctaccagatt gcgggtccgc tccagcatca 27480ccgggaagcg gtataacgac aatgtgccgg gcagcactgc actaccgccc tgagaggcct 27540gtaccatact ggtgagcagc gctttcggat cggtaggctc ggcgtaaatc gccagcgata 27600acggctgtcc gtcaatggaa agattatggc gcaggttaaa caggcgcaaa cgcagggttt 27660gccagtaatc ggtgagcgcc gggttatatt ccggcaggaa caaacccacc aacgagttag 27720cggtacggag attcttggaa accccaccac ggcccagcat cgtaagatcc tgctgataag 27780ccgcctgcac ggtttgactc gccgccccgg aaagggacgg tgctgcccac tgttggctac 27840cgtaatcctc cggctcatca ccgagcaatt ctaaagtacg cacataccac attttggctt 27900cattcaacgc atcgcgggtc agttctcgat aggccatatc gccgcgcaga ataagttgat 27960
ccaacaggcg cataaaggtg gcaatcttgt agtgcattgg gtcattttgg gcgacggcat 28020ccggatcgat ggcatccagc ggattggcat tccaggaggt ggtctcttcc agcggccggc 28080agttccagat ccagggggcg atttctccgt taacgatata gccggcggga ttgtagacgt 28140agtttatcca ttgtgtggct tcgtcgaatt gtttttcctg tagcaaacgc tggaagcaca 28200tcatcggggt gtaatagaac aattcccagt aatagagggc gctggcacta ttgaaatcca 28260tcggggcgga atagcccgtt gcgatagaaa cattcaaaaa tcctttgtat ttcttgatat 28320ttttcacgat cccctgttgc gtcattcctg aatcatgatc agcatcgtta attaatacaa 28380attgctgttt tgtctcatca aaataaaaga aagcagattc ccaagtgttg tcataggtaa 28440ttttctggta tccaaccccc aatctgacac cttcatgcat gtaataccct tcggcataag 28500ggacaaacag tgtcatactg gtttccgacg tatcggataa cattccgctg taataaggct 28560gccttcccgt gttaccgcca acattcccaa tatggatttt aaaccaccgc tcatcgccat 28620gttcagcagg gtcatattta ggcagaacaa agttggcaaa gaagccttct cccaacggag 28680gttccggtaa ccgctgggtt tccattgtca ggatagtatc aatgcccgtg tttgctctgg 28740ataccagttg agaagccagc agggtattaa gacgaatacg atacaccccg agctgcatat 28800attgggcacc cgaatgagtt tcacgcagaa acagaatatc ttccggatta taatttaccc 28860gtttcaccga taatgtttgc ttgatcttac ccagcactcg cccgtctttg gctttggtct 28920caaaaacgat atccagagga gcaatattat tggtaaaggc caacgatgaa gcatcgattt 28980ccagtggctt aaaggtgtac ggcatagcat caaaactgtt tgccggcaag gaagcaatat 29040ggtcactggc cgtaaaggtg tgggttttac tgccagccat caccgtaatt ttgatatcgg 29100tattgttaat gcctgtatca atatccagcc agccggatga ttgataggaa ctaaatatct 29160ggaagccctg agaattatta ccatcaacag cataactgca ctttttaaaa tcactggttt 29220tattgctacc aaccgtgaaa acggtgttta aaattgtgtt tggagaaaat ggaaactcga 29280
acaatctggc ataataatta ttttcaactg gtgttagaat caaacgccta gtgtaatctg 29340cgttcatcaa gtggccttga actgatgcaa tatagttttt cgttttatta taaacggtga 29400tcggcccccc cagatcagag taaccgccat aatgtttaac ggtatttgcg atgataaatg 29460cattgccgta ctgggacttt ccatccaccc ccgtcagttt catggcgctg atttgtttgt 29520ttctgatgac attgccactg ccatcatatc tgacagtgaa agcggcgtta tgtagcgtaa 29580tagcaaggtt atcgctggag tatttactgg ttatctgcgg aatattcccg ttctccatca 29640ccgtcagact atcatcaccg atggcagaac ccatattcaa cgaggcaggc acttcaaaat 29700cctgcgcgaa acgatagctg gcctttctta ccaagtcgtt gccttgagta tgaatgatat 29760caaaggtatt tttcagttgg ctgtaacggc tgagtgctgt gttctccatc tttttgaagg 29820agccatcgcc gtaaatggtc atgcctgcca catttttatt gctgccgcca aaatccgagt 29880aactcttccc ggttttgtag acaaacacca gcagagtgtc ctcgccctga aagcctgatg 29940cggccagcgc cagccgttca gtgtcaggtt ttttgtcagt gaccgcctcc acctgcgttg 30000tgatatcgta agaccagggg gcactccaac tgccatcatg acgcagaaac gccagtttca 30060gagtaaaacg gtcataggtt tccaccggat cagtaccatt tttcgccact tcctcttttt 30120ctacccagat aaggtgcaaa cgttccctga atatgaccgg acgtattgca tccttgtagg 30180ggttgaccgc tgtatcaatc ttcgtccact ctttccaggc attggcggcc agttcacccg 30240cctgcatccg tgatatatcc acgttacgcc agtagtattc cggcaggttc tcccgcgttt 30300ggccgacaaa ccaggtcagt ccggtgttgc tgttgacgtt gtcgtgatag gcgctgacaa 30360ctttcagatc cgccacggtt tcaaagcggg tcaggtaagt tttaaaggca tcctccactg 30420tgtcccggct aagtttactc tggctgatat tttccagcag ttcatccatc atccgggtct 30480gcccgatacg ctgggttggg tcaatgtaat tttccggata ataaaccagc cgcgacaccc 30540cgccccaggt gctgtaacgg ttattcaccg tccagtcggt aaaaaactgg cgggttgaca 30600
catcggcacg ggcattaggc tctatccgat tcagcgcccg gttgatgtag agctgaatac 30660cggcaatggc ctctgccagt cgggtggttt ttatggcaga agagacctga ttatcaatca 30720ggaaatagct gtacaggtca tcccggctgt gcagggacac cccttctggc tggatattcg 30780ccagaaacca attgcacagc acgctactca ggcgctccgc ggtataatcc gccagcgtct 30840gagcctgttg tgtactgagt ccggcttcca tattttctgc cagtgtctgc cactcatccc 30900aggaaggcag attcgactcg gctttgttta atgcagtcac gtaacggata ttcaccagcg 30960tacggataac cgacggcatc gtgtgcagtg ctgatgccac atctatccac tgcaacacgg 31020tgttgatatc ctgccaacac tgaagctggt tcacgccggc ggaaaccatg gcctgcgtta 31080ccatactgat gtccagcccc atcacggagg ccagtctgtc ggccgtgagt gtctgctggc 31140gcagcatatc cagcgtgtca gagccgggat tgcccagccc attaatccac tggtggaatc 31200ggtagagtga gaatagcgta tcaatattgt gctgtccggc aggttgattt tttgccccca 31260gcacggcgaa tccggagatg accagcacgg atagctccgc ttcactgagg cgcagtgtct 31320gtacggaaag cgataactgt gccatcacat ggcagaattg taccaattgg gtggtttcat 31380tggcatttaa cgactctttc aataccagtg tcataaaccc ggcaatatct aagccacccg 31440gccgcaggtt atcggtccac aacaggatat accgtgccat atccggtgac gccagatgca 31500gcgttgcagc aataaacggc gcgagaattt cagcctgcag ctcccgattg tgactctgtg 31560ccatatcttc actaatactc ggtcggaggt tattgagcag attactgatt tccggtgaaa 31620tattcccgct aaactctggc gtacataata accagatcgc ttcagtggtg atttccgcct 31680cagtcagcca ctgcgtcacc tgatacagcc agataaccag ccgtggcaac tccccggaag 31740acaaagaagc cgttgttttg ccattgaacg gcgaaagacc ataaagcata cacagttcat 31800tgaccgtcag ctgatggaca cgggccagta acgtgaggcg atacagtgaa gagataacga 31860agacagaaag tgtgatggta ttttgggcgt ccagcacacc cgccagtttg cctaactgat 31920
acagttcacc actgttgacc cccagaccac gcatcagggc tgaacgggca aaggtagatt 31980gctcttcatc cggatcaatg ctgaccgtgt tgccgtcggc ttcaaagatt ttccctttca 32040gcggcggtgt attaaagaga cggttaaaat gactgacact gtcatcgtcg gcatattgat 32100taatgaccga tccgttcagt acctgtgcat catcaaagct cagtgcataa cggtgactgt 32160agaacagagt atagaaaact ttggtcagaa cggagtcgtt gatgatgcct tgtgcattgt 32220cactgcgtac gatagtttgc agttcattcg gtgaaagccc gctagtcagg cacaagcgaa 32280tggctttatt cagtttgagc gcaaatatag tcaggggata agactcaaaa gtgaatattc 32340cgccgccctg atttgtggcg ctggtggaag acgtatagcg ataggcgtat atctttacac 32400cgtttttgta ttcagaatca gatatgttac ttagataatt acttttaaaa ttcgtattgg 32460ctattagagg accggaaagg ctgccgacaa tgccacttgg ccctgcgttt tttctaagag 32520tagccccaaa ttctcttgat accttaaaat tagcacgtat aaagaactga ttatttcctt 32580catacatcaa atcaaagtaa tttatatttt tatcataatc atctgttttt acacgtgtta 32640ttttgtaagc ttcgagttta ctttcattat tgaccactaa acccgttgag atattatcca 32700cataagcaga ggtgctgtca gaatagccat tctgcaacat cccgaggtat ttttgcacct 32760cagaaagttc aagaccataa tacttggcta tccatgattg tgacgcgaaa ttttcgggcg 32820tgatattttc actgaagttt tgcgcaaata aagcatcagc gttcttttcc gtaatctctt 32880cggtcaaaat gttataaagc tccggagaaa tattggccag aatcgccagt aatgaagccc 32940cttccgcctg ccccatcacc tcaggattac gggacagcgc tgacagtgta ctgtcatggg 33000tcataatgac ctgacggata gtctcgtaag gctgatggta aggggtatca atggcctgac 33060ggtaagttga caggctctcc atcaatgcgt ccgaatcacc tccggtcttg cgggtaatat 33120gctccagcaa cagttcgtta gacagtgtca gggtggaaat ttctgtatcc atattactct 33180ggctcagagt cagatcagcc agatccggac ggcgattatc aagatgataa gcagagcttg 33240
aaaaatgtaa gtccttcgct tcacgataca attcggtgag atagccagcc ggtgaaaaca 33300tggaagccac tgaacccggt ttcacaaagg aagaagaacg ggcaccaaac atttcatcat 33360aactgcgtga aacgctgtct cgttcaatac cgagtcggat agcaccggat aattgtgggt 33420tggcacgggt aaaaatacgc gcttccagca agcgattatt ttttttctgc tctatagttt 33480catgatagag atggcgagcc tctccccaac tgagctggtc atcaaagatt tttctcagtt 33540cactgaagga taaatattgc agatccgcaa gagtcatcgt ctgaccgtcg cgagtgggac 33600tgattttatt gagtaataca gccgtgctat acataataac ctcaattatt ttataaaata 33660gtgttgtcag ttaagagttc atctgaagat ttagtgctta ttttgtaagt cattattcta 33720ttcacattgt aattatttgt tttatctgag attaatgata ttaaagagga tgctattgta 33780aatggcggaa tagaatacga ttttctactg aaatttcatt ttaatcataa aatttataac 33840tgactttaat gttacagtcg taatcgatat tgtgtcatgt tggcatcctc ttcatctgcc 33900ttaaaataaa gtagggtacc aaaaggaata catacttgaa tccaagattg agcacaaatc 33960cacatattca gcttattaaa gataattaaa ttttatttat cataaataaa taggataacg 34020gccctggatt ctgaccaggc gaggccaaaa gtcgatgaag ctaagttacg gttgaacaaa 34080tttgtttact ggttaaatgg gcacaaactc tttatataaa taaatagcat attgtaacga 34140gtaattaaaa aatgaaaatc cagcttacct ggttattcat tcattaacaa aacacaaaat 34200atttatgcca acggcactta gaattaaata attttcttta tcaactttta cgttaacttc 34260atttgataaa agtaagatcc catgattttt caagatcctt attcggttat aactgaccag 34320attgggaaaa tcaaccttaa tgtctcatgt gaaataaaat attgtccaag tgatttattg 34380ttttgtatta taattcagtc tcttttatca acatctaact taagtcctca agagaaatta 34440attgcaaatc ggtcaccata accggctaat aatgtattga tctcatattc cattgtttcc 34500tgagtccagg tgataaaacg tcgccagtgg tatttagcct ccctccagac gatttcaatc 34560
aaattcagtt ctgggctgta ggcgggaagg taaagtaaaa gcaggttgtg ctctcgtaac 34620cagcgatttt taagtttttc atcgatccca tgatggatag gcgcattatc taacacgaca 34680aatgtcaggc gatgctcgcc ttgttgggcg acctgctcta aaaaatcaat gacattactt 34740cgcgtgacac tgcttgatat tatctgataa aacagcctgt tatcagtgta atttagcgca 34800cctagcactg accgtctgac agagctttgc ggctctgctt catggggctt acctcgtgga 34860ctccatccat attggaccgg tgggcaggcg gaaaaacccg cctcatcaag atagagcagc 34920cgataatgac ctgcccgtgc gcccgcctta attttattca gtaaggcggc tttttcagca 34980aattccgttt tattgcgttt tttttaagcg acaggcgggg gcgtttatag gggagtccct 35040gttttttcag ggtattcgcc agcgtttcaa gcgtacaggg cagggaaccc tgcctggctt 35100cgacgcacgt cagggactct gcgctggcgg cttcgagcgc agtggcaatc atgtcaggcg 35160tcatggcgag ataccggcct ccggcatgac cccctaataa tcccgctatc cctgaatggt 35220gccacatgtg aacccaatta tagataaccc ggagactgca tccgatttca gcggtgatct 35280gggacggctt gatccctctg gcaagcatga gcaaacccgt tcctcgcgta cgaatgtccc 35340ggtgtgggtg attcaaagcg agtggttgca atgtgattcg ttcaggctca gaaaggatta 35400tcttcgagtt cataagaaca ggcagaaagt caggttatcg tgttatcgat tataacagta 35460atgcagataa tttatctgat taacttaatt tatttttgca ataaacgttt ttagcaccat 35520gaaaaataat aagaaagaat cctgattatg ttgagagagt atacaaaagt ataaaaatgg 35580cgaattaaat caccattctg atagtgacaa ttattccctt acttttatag tataattttt 35640attgaactct ttccctgcgt acattgtacc caaagtaaat cctaccactt caatttttat 35700caattctgtc ttctttgggg tacctgttat atttatatga tgataatctt ttcttatttc 35760tttttttcca ccctatagga aagaactatc ttttggattc catgttagtc ctgagccagt 35820aggatgaatt tttacccaaa cgcttttttc attcaatgca cctcctgtga tggtaattga 35880
tgcctgatat ggttcattta tcactgcatc aggaagaaac tctgattttg gtaaaacttt 35940tggcgttgga ttaccacaac cataaagtaa aaaaataaaa ataattaata tttttttcat 36000gttatttcat tggtttaaaa accaaccctc aaaaaatatg gttgagtaca taatctagtg 36060atttgttttt tttaatttga tgttccactt taccccatga aaacagattt aaatttacat 36120attgattcag atctgaatta tttgtgatat tttctttctc atagttttct actactcctt 36180cccaaactat ccaatgattt tttcctgatg tttctatgtc accaaaatct gataacattc 36240ctgctgaaat caaagtaaca acatgatatc ctttgttata gtaatcacta agagttacta 36300tgtcatttat attagaatgg gataagccga cattactaaa tactttttca taccctgatt 36360tttcaaacca ttctgtcaat tttccccaca ttgtaatacc agctacttca tcatcaacct 36420catcataact catcatcata ttttctgaat ctcttaggct tgccaatgtc agccaatcta 36480acccagatat tctttcacca tattgattat aaaaagtacc tttaggatgt cggcaaccct 36540cacccagctt aatttccagt tgaccaattt tagttcggcc atattgccat aattctcgtg 36600cagcttgctc ataaatatct ggtctatcta ttggcaggca ataaaaaaaa gcggcagggc 36660cacataaact tgctccattt tgatccggat aagttctttt cgataccctg tcctgtattt 36720atgattcaat tttacttttt tcaaatggat cgtgtgggtg accaatggga tattctttgg 36780caataaaagt ccgttcagga atggtgattt ttaattcgac ggtattatcc ccgtcgctga 36840caggttttgt ttcaactgta ctttcaaaat aacaggcatt ttcccgtttt ttctccgaac 36900aggctttacc ggaagttaat tcaccgaggg tatacatttt cttaaaatgg gtttgcagtg 36960ccggaaatat atttgagcca tgtttgcggt gaagcataat ttgcataata taaatcacag 37020aaagatcgtc gtctatttcc tgcgtgatat gctgttgtgt ttcataatta ctctgtagtt 37080tctcctgaaa aacatcaagc aagatatgga aatttccttt tgcattctga cgataaatgg 37140ttaagtcata aagcaggttt tcaacaggta cgttggattc tatttttgtc tgaacggtat 37200
aggctggcat ggtattaatc ctttaaaata tgaaattcaa gtttattttt gtcatccgta 37260agatgccatt gggtgtaacc ttgtttatca gtctttcctt cttttatctg accatccggc 37320aggcaaaccc gatacttgcg ttcggttaaa agattgccgt catcatccac acaacgataa 37380cgggcatgat gtttagcggg ttttaccggg gtttcttcaa ccagcggctt cttaagtggc 37440ttaacattcg agaccccaga taaaagcggc ttgccttctt cacttccggc taattgcgta 37500atgctgtagc ccgaagtccc tgcggcgatg tggttgcatg aaatggactt ataatcgagc 37560atcaccactt catggggtat cgcaccatta tcattgattg aatgcggata attataggaa 37620atatccacaa tcgtggcact ggtcagcttg atttcataaa agaactccag ttgtcccatc 37680tggcttgtcc tgtaatgcac aaaactggca tccagcaaac aggggagagg atttatcaat 37740gggtttcaca aaactgacgg gttgatgatt aacattctgg tcacggctca tcgaatgatt 37800caggctcaat acctgtattt gatcacacag gccagctggt ttgcccgttc gttaagctgg 37860cggtaggtca gtgttgcgcc ttcaaacacc agtgccccgt tatccggtgt cctttccacc 37920tgagcttcaa acagttgtgg cagggttttg tcctgtggat aaggcgcatc ggtctggttc 37980caggtatgca gcagggtatg gcgctcctgt gcggacagaa tatccagcgc ggacagcggt 38040tgtttctggt ctgccacaaa ggcttccagt acccgttgat agctctctgt cagcctgacg 38100atggtggttt cattaaacag gctgactgcg taattcaggc aaccggtaat ctcggtttgt 38160ccgtcggaca taaacaggct gaggtcaaac ttggcggggc tgtatagcgg ctcatccaga 38220gtcaccggcc tgaatggcag gcggttgtct gacggatttt ctccaaagct ctgtaaacca 38280aacatgatct gaaaaatcgg gtggcgggcg gtatcacgtt caatattcag ggcatcaagg 38340agctgttcaa acggcatatc ctgataggcc ttggcttcgg caacctgttt atgggtctgc 38400tcaatcaggg cttccacgct gacagtctgt tgcaactgtg cccttaagac cagtgaattg 38460acaaacatcc caatcagggg ctgagtctgg gcatggtggc ggttatcggt tggcgtcccc 38520
agtacgatat cgttttgccc ggataatttt gccagcgtga cataaaaggc actgagcaac 38580acggtataca gggtggtttc ctgtgttttt gccagactcc ttaactgttc agatagccgg 38640gtattcagcc caaaactgaa attacatccc tgataattca cctgagccgg tctggggtaa 38700tcggttggca aggccagtga ttcatagttg gctaaagcct gttgccagta agcgagttgg 38760cgttcgcgcc ggtccccttg taaatagttg cgttgccatg cggcataatc gccataggtg 38820atatccagcg ctgcaagctg gctgtcgcgg ttttcccgca aggactggta aatttccgcc 38880agttcagcca taaagatatc aattgaccag ccatcaatgg cgatatggtg ccataacaat 38940aataaatagt ggctgtcaga aaccggatag tgacacaggc gcagactggg ttctgtggtc 39000agatc 39005<210>7<211>1533<212>DNA<213>嗜线虫致病杆菌<400>7gatcaggtat tcaatcaacc caaactgttt gatgaacctt tctttgttga taatcgtact 60tttgattaca acgccattcg tggtaatgat gcacgaacaa ttaagcaact gtgcgccgga 120ttgaaaatca ccgtagccac cttccaattg ttagctgagc aggtaaacac cgcctttcat 180ctgccatccg gcaaattaac ctgttcactg cctgttattt cagcgcttta tcgtctggtg 240actgttcctc ggttatttaa tttaaccgct gaacagggca tgatgctgat taacgcatta 300aatgccagcg agaaattctc acctcatatt ctggctggtg agcctcgatt aagcctgtta 360acaacagagg gttcagatac cacagaggtc gatttattgg atgttattct gatgttggaa 420gaagttgctg tctggctgca acagagcaaa ctgaaaccgg aagaattctg cctgatgctg 480caaagtgtta tgttgccggt ggttgccacg gacagcagtg tgacattctt cgacaacctg 540
ctgcaaggca ttcccaaaac cttactcaca gaagataact tcaacgcagg ggatatcccc 600agactccctg aaggagaaac ctggtttgac aaactttcga tgctgataac cagcgatgga 660ctcgtcaacg tttaccctct cagttggggc cagagtgatg aagattatct gaaatcagta 720ttgacacctg tcgtcgaaaa aatcattagc gatccaaaca gtgtgattat cactgtttcc 780gcattaacac aggtcattac tcaggcgaaa actgcgcagg aagatctggt ttccgccagc 840gtgacacggg aatacggtac tggacgtgat atcgttcctt ggttattacg ctggattggc 900agcagtgttc ccgatttcct tggcaaaatt tatatacaag gcgcaaccag aggcggacac 960ttgcgcactc cgccggatat cagcgctgaa ttactgcata tcacctatca tctggcgatg 1020aataacatgc tgattaagca gttacgactc aaagctcaaa tcatttcatt acgtatcatc 1080atgcctgaat ggctcggatt accaacgata gatggcagtc cgctatccgt gcatgaaatt 1140tgggcactga gccggttccg taactgggcg accagctcat tgttcagtga agacgagtta 1200atcgagtatt ttgcttttgc caatcagccg gagcaggacg ttcgtaacga tgaagatttt 1260aatcgggact gtgctgaaaa gcttgccgac atactggaat gggatgccga tgaaattgag 1320ctggcaaccc gacattttga tcctgcccca gcacgtgcca gaaatatggg acaaattgac 1380tggctgcgtc gtgtcatggc gttgtcgcgt cagactggcc tgtcagtgac accgttaatg 1440acagccgcaa cgttaccgcc tttcccgccc tatgaccaga taacccatgt cggtgaagcg 1500gtgattgcgg caacccagta cccatcagag gag 1533<210>8<211>511<212>PRT<213>嗜线虫致病杆菌<400>8Asp Gln Val Phe Asn Gln Pro Lys Leu Phe Asp Glu Pro Phe Phe Val1 5 10 15
Asp Asn Arg Thr Phe Asp Tyr Asn Ala Ile Arg Gly Asn Asp Ala Arg20 25 30Thr Ile Lys Gln Leu Cys Ala Gly Leu Lys Ile Thr Val Ala Thr Phe35 40 45Gln Leu Leu Ala Glu Gln Val Asn Thr Ala Phe His Leu Pro Ser Gly50 55 60Lys Leu Thr Cys Ser Leu Pro Val Ile Ser Ala Leu Tyr Arg Leu Val65 70 75 80Thr Val Pro Arg Leu Phe Asn Leu Thr Ala Glu Gln Gly Met Met Leu85 90 95Ile Asn Ala Leu Asn Ala Ser Glu Lys Phe Ser Pro His Ile Leu Ala100 105 110Gly Glu Pro Arg Leu Ser Leu Leu Thr Thr Glu Gly Ser Asp Thr Thr115 120 125Glu Val Asp Leu Leu Asp Val Ile Leu Met Leu Glu Glu Val Ala Val130 135 140Trp Leu Gln Gln Ser Lys Leu Lys Pro Glu Glu Phe Cys Leu Met Leu145 150 155 160Gln Ser Val Met Leu Pro Val Val Ala Thr Asp Ser Ser Val Thr Phe165 170 175Phe Asp Asn Leu Leu Gln Gly Ile Pro Lys Thr Leu Leu Thr Glu Asp180 185 190
Asn Phe Asn Ala Gly Asp Ile Pro Arg Leu Pro Glu Gly Glu Thr Trp195 200 205Phe Asp Lys Leu Ser Met Leu Ile Thr Ser Asp Gly Leu Val Asn Val210 215 220Tyr Pro Leu Ser Trp Gly Gln Ser Asp Glu Asp Tyr Leu Lys Ser Val225 230 235 240Leu Thr Pro Val Val Glu Lys Ile Ile Ser Asp Pro Asn Ser Val Ile245 250 255Ile Thr Val Ser Ala Leu Thr Gln Val Ile Thr Gln Ala Lys Thr Ala260 265 270Gln Glu Asp Leu Val Ser Ala Ser Val Thr Arg Glu Tyr Gly Thr Gly275 280 285Arg Asp Ile Val Pro Trp Leu Leu Arg Trp Ile Gly Ser Ser Val Pro290 295 300Asp Phe Leu Gly Lys Ile Tyr Ile Gln Gly Ala Thr Arg Gly Gly His305 310 315 320Leu Arg Thr Pro Pro Asp Ile Ser Ala Glu Leu Leu His Ile Thr Tyr325 330 335His Leu Ala Met Asn Asn Met Leu Ile Lys Gln Leu Arg Leu Lys Ala340 345 350Gln Ile Ile Ser Leu Arg Ile Ile Met Pro Glu Trp Leu Gly Leu Pro355 360 365
Thr Ile Asp Gly Ser Pro Leu Ser Val His Glu Ile Trp Ala Leu Ser370 375 380Arg Phe Arg Ash Trp Ala Thr Ser Ser Leu Phe Ser Glu Asp Glu Leu385 390 395 400Ile Glu Tyr Phe Ala Phe Ala Asn Gln Pro Glu Gln Asp Val Arg Asn405 410 415Asp Glu Asp Phe Asn Arg Asp Cys Ala Glu Lys Leu Ala Asp Ile Leu420 425 430Glu Trp Asp Ala Asp Glu Ile Glu Leu Ala Thr Arg His Phe Asp Pro435 440 445Ala Pro Ala Arg Ala Arg Asn Met Gly Gln Ile Asp Trp Leu Arg Arg450 455 460Val Met Ala Leu Ser Arg Gln Thr Gly Leu Ser Val Thr Pro Leu Met465 470 475 480Thr Ala Ala Thr Leu Pro Pro Phe Pro Pro Tyr Asp Gln Ile Thr His485 490 495Val Gly Glu Ala Val Ile Ala Ala Thr Gln Tyr Pro Ser Glu Glu500 505 510<210>9<211>4173<212>DNA<213>嗜线虫致病杆菌<400>9
atgagttcag ttacccaacc tattgaagag cgtttactgg aatcacagcg cgacgcactg 60ctggatttct atctcggaca ggtcgttgcc tattcacctg acatgacaag tcagcgcgac 120aaaattaagg atattgacga tgcctgcgac tacctcctgc tggatctgct gacttccgcc 180aaagtcaaag cgacacgact ttcacttgcg accaattcat tgcagcaatt tgtgaaccgc 240gtgtcactga atattgaacc cggtttgttt atgaccgcgg aagagagcga aaattggcag 300gaatttgcga atcgttataa ttactggtct gcggatcgct tattacggac ttatccggaa 360agctatctgg aacccctgtt acgcctgaat aaaacagaat tcttcttcca actggaaagt 420gcccttaatc agggaaaaat taccgaagat tccgtacaac aagcggtgct cggttatctg 480aataattttg aagatgtcag taacctgaaa gttatcgcag gttatgaaga tggtgttaac 540atcaaacgcg ataagttctt ctttgtcgga cgtacccgta cacagccata ccaatattac 600tggcgttcac tgaatctttc gatacgccat cctgataccg atgcgttatc tcccaatgcc 660tggagcgagt ggaaacctat tgacctgcca ttgggcagcg tagaccccaa tttgatacgc 720cccattttcc tgaataatcg cctgtatatt gcctggacgg aagttgaaga acagtctgaa 780actaaagata caactgcgtt atcactgcat aaccaaaacg ttgagcctag tgcgggtgat 840tgggttcctc ccacaccgtt cctgacccgg atcaaaatcg cttatgccaa atatgatggc 900agctggagta cacccaccat tctgcgcgaa gacaatctgc aataccggat ggcccagatg 960gttgctgtga tggatataca gcaagacccg cataacccgt ttctggctct ggttccgttt1020gtccgtcttc aggggacaga taagaaaggt aaggattatg attatgacga agccttcggt1080tatgtctgcg atacactgct ggtagaaatt actgatttgc cggatgacga atatgctgat1140ggacgaaaag gaaaatatgt cggcaacctg gtctggtatt actcacgtga acacaaggat1200gcagaaggca atcctatcga ttaccgtact atggtgctct atccggcaac ccgggaagaa1260cgctttccta ttgccggaga agccaaaccg gaaggaagcc ctgattttgg caaagacagt1320
atcaaactga ttgtcaattt tgttcatggc actgatgaca cactggagat tgtcgctcaa1380tctgacttta agtttggtgc gatagaagat catcaatatt acaacggttc tttccggctg1440atgcacgata atactgtctt ggatgaacaa ccactggtac tgaacgaaaa agttcctgat1500ttaacctatc catcaatcaa gctggggtcg gataatcgaa tcaccctgaa agccgaactt1560ctctttaagc ccaaaggtgg tgttggcaat gaaagtgcca gctgtactca agagttcaga1620atcggtatgc acattcgcga actgattaaa ctcaatgaac aggatcaggt gcaattcctt1680tccttccccg cagatgaaac tggtaacgcg ccacaaaaca ttcgccttaa tacactgttt1740gcaaaaaaac tgatcgccat tgccagtcag ggtatcccgc aggtactgag ctggaataca1800cagcttatta ctgaacaacc catacccggt tcattcccta cgccgattga tttaaatggc1860gcaaatggga tctatttctg ggaactgttt ttccatatgc catttctggt cgcgtggcga1920ctgaatatcg aacaacgatt aaaagaggcc accgaatggc tgcactatat ttttaatccg1980ctggaagatg aacttgttca ggccagcaac caaggtaaac cgcgttactg gaattcacgg2040ccaattattg atcctccacc caccgtgtac cggatgttaa ttgaaccaac cgatccggat2100gccattgcag ccagtgaacc cattcactac cggaaagcaa tattccgttt ctatgtcaag2160aatctgttag atcagggaga catggaatac cgtaagctga catccagtgc acgtactgtc2220gccaagcaga tctatgactc cgtcaatatg ttactgggta ccagccctga tattctgctc2280gcggcaaact ggcaaccccg tacgctgcaa gatgtggctc tgtatgaaaa cagtgaagca2340cgggcacagg agttaatgct tactgtcagc agcgtgccac ttctgcctgt gacatatgat2400acatccgtct ctgccgcacc gtctgattta tttgtcaaac ctgttgatac ggaatatctc2460aaactgtggc aaatgttgga tcagcgtcta tataacttac gtcataacct gaccttggat2520ggtaaagagt ttccggccgg attatacgat gaacccatca gcccgcaaga tctgctcagg2580cagcgttacc agcgtgttgt ggctaatcgt atggcgggca tgaaacgccg ggcaatcccg2640
aattatcgtt tcaccccgat catgagccgg gcaaaagagg ccgcagaaac gctgattcag2700tacggcagca cgttactgag tttgctggag aaaaaagaca ataccgattt tgaacacttc2760cgtatgcagc agcaactggg gctgtacagc tttacccgca atctgcaaca gcaagcgatt2820gacatgcaac aggcttcatt ggatgcactg accatcagcc gacgggccgc tcaggagcgc2880cagcaacact ataaatcgct ctatgatgaa aacatctcca tcaccgagca ggaagttatc2940gcattacaat caagagcggc tgaaggtgtg atcgctgccc agtcagccgc cactgcggcc3000gctgtggcgg atatggttcc caatattttc ggtctggccg tcggggggat ggtctttggc3060ggtatgcttc gggcaatcgg tgaaggaata cgcattgacg ttgaaagtaa aaatgccaaa3120gccaccagcc tgagcgtgtc agaaaattac cgtcgccgtc agcaagaatg ggagctgcaa3180tacaaacagg cggatatcaa cattgaggag atcgacgcac agattggtat ccagcaacgc3240caactgaata tcagcacaac ccaactggca caattggaag cccagcatga gcaggatcaa3300gtcctgctgg agtactattc aaaccgtttt accaatgatg cgttatacat gtggatgatc3360agccaaatct ccgggcttta cctgcaagcc tatgatgcgg ttaattccct ctgtttactg3420gccgaagcct cctggcagta cgaaacaggt cagtatgata tgaatttcgt ccaaagtggt3480ctctggaatg atctttatca ggggctgctg gtcggagaac atctgaaatt agccttacaa3540cggatggatc aggcgtattt gcaacataac accagacgtc tggagatcat aaaaaccata3600tcggtaaaat cattactgac atcatcacag tgggaaattg gcaagagtac gggttcattc3660actttcttac tgagcgccga aatgttcttg cgcgattatc cgacccacgc tgatcggcgt3720ataaaaaccg tagcgctgtc attgcccgca ttgctggggc cttatgaaga tgtacgggct3780tcactggtac aactcagcaa tacgctttac agtactgctg acttaaaaac tatcgattat3840ttgcttaacc ccttggaata caccaaaccc gaaaacgttt tgctgaacgt acaggctaat3900caaggtgtgg tgatttcaac ggccatggaa gacagcggca tgttcaggct caattttgat3960
gatgaacttt tcctgccttt tgaagggaca ggcgccattt cacagtggaa gttggaattc 4020ggttccgatc aggatcagct gctggagtcg ctgagcgata ttatcctcca tctgcgttat 4080accgcgcgtg atgtgagtgg cggaagtaat gagttcagcc agcaggttcg tagccgtctg 4140aataaacatc aattaaaaca agacaattct aac4173<210>10<211>1391<212>PRT<213>嗜线虫致病杆菌<400>10Met Ser Ser Val Thr Gln Pro Ile Glu Glu Arg Leu Leu Glu Ser Gln1 5 10 15Arg Asp Ala Leu Leu Asp Phe Tyr Leu Gly Gln Val Val Ala Tyr Ser20 25 30Pro Asp Met Thr Ser Gln Arg Asp Lys Ile Lys Asp Ile Asp Asp Ala35 40 45Cys Asp Tyr Leu Leu Leu Asp Leu Leu Thr Ser Ala Lys Val Lys Ala50 55 60Thr Arg Leu Ser Leu Ala Thr Asn Ser Leu Gln Gln Phe Val Asn Arg65 70 75 80Val Ser Leu Asn Ile Glu Pro Gly Leu Phe Met Thr Ala Glu Glu Ser85 90 95Glu Asn Trp Gln Glu Phe Ala Asn Arg Tyr Asn Tyr Trp Ser Ala Asp100 105 110
Arg Leu Leu Arg Thr Tyr Pro Glu Ser Tyr Leu Glu Pro Leu Leu Arg115 120 125Leu Asn Lys Thr Glu Phe Phe Phe Gln Leu Glu Ser Ala Leu Asn Gln130 135 140Gly Lys Ile Thr Glu Asp Ser Val Gln Gln Ala Val Leu Gly Tyr Leu145 150 155 160Asn Asn Phe Glu Asp Val Ser Asn Leu Lys Val Ile Ala Gly Tyr Glu165 170 175Asp Gly Val Asn Ile Lys Arg Asp Lys Phe Phe Phe Val Gly Arg Thr180 185 190Arg Thr Gln Pro Tyr Gln Tyr Tyr Trp Arg Ser Leu Asn Leu Ser Ile195 200 205Arg His Pro Asp Thr Asp Ala Leu Ser Pro Asn Ala Trp Ser Glu Trp210 215 220Lys Pro Ile Asp Leu Pro Leu Gly Ser Val Asp Pro Asn Leu Ile Arg225 230 235 240Pro Ile Phe Leu Asn Asn Arg Leu Tyr Ile Ala Trp Thr Glu Val Glu245 250 255Glu Gln Ser Glu Thr Lys Asp Thr Thr Ala Leu Ser Leu His Asn Gln260 265 270Asn Val Glu Pro Ser Ala Gly Asp Trp Val Pro Pro Thr Pro Phe Leu275 280 285
Thr Arg Ile Lys Ile Ala Tyr Ala Lys Tyr Asp Gly Ser Trp Ser Thr290 295 300Pro Thr Ile Leu Arg Glu Asp Asn Leu Gln Tyr Arg Met Ala Gln Met305 310 315 320Val Ala Val Met Asp Ile Gln Gln Asp Pro His Asn Pro Phe Leu Ala325 330 335Leu Val Pro Phe Val Arg Leu Gln Gly Thr Asp Lys Lys Gly Lys Asp340 345 350Tyr Asp Tyr Asp Glu Ala Phe Gly Tyr Val Cys Asp Thr Leu Leu Val355 360 365Glu Ile Thr Asp Leu Pro Asp Asp Glu Tyr Ala Asp Gly Arg Lys Gly370 375 380Lys Tyr Val Gly Asn Leu Val Trp Tyr Tyr Ser Arg Glu His Lys Asp385 390 395 400Ala Glu Gly Asn Pro Ile Asp Tyr Arg Thr Met Val Leu Tyr Pro Ala405 410 415Thr Arg Glu Glu Arg Phe Pro Ile Ala Gly Glu Ala Lys Pro Glu Gly420 425 430Ser Pro Asp Phe Gly Lys Asp Ser Ile Lys Leu Ile Val Asn Phe Val435 440 445His Gly Thr Asp Asp Thr Leu Glu Ile Val Ala Gln Ser Asp Phe Lys450 455 460
Phe Gly Ala Ile Glu Asp His Gln Tyr Tyr Asn Gly Ser Phe Arg Leu465 470 475 480Met His Asp Asn Thr Val Leu Asp Glu Gln Pro Leu Val Leu Asn Glu485 490 495Lys Val Pro Asp Leu Thr Tyr Pro Ser Ile Lys Leu Gly Ser Asp Asn500 505 510Arg Ile Thr Leu Lys Ala Glu Leu Leu Phe Lys Pro Lys Gly Gly Val515 520 525Gly Asn Glu Ser Ala Ser Cys Thr Gln Glu Phe Arg Ile Gly Met His530 535 540Ile Arg Glu Leu Ile Lys Leu Asn Glu Gln Asp Gln Val Gln Phe Leu545 550 555 560Ser Phe Pro Ala Asp Glu Thr Gly Asn Ala Pro Gln Asn Ile Arg Leu565 570 575Asn Thr Leu Phe Ala Lys Lys Leu Ile Ala Ile Ala Ser Gln Gly Ile580 585 590Pro Gln Val Leu Ser Trp Asn Thr Gln Leu Ile Thr Glu Gln Pro Ile595 600 605Pro Gly Ser Phe Pro Thr Pro Ile Asp Leu Asn Gly Ala Asn Gly Ile610 615 620Tyr Phe Trp Glu Leu Phe Phe His Met Pro Phe Leu Val Ala Trp Arg625 630 635 640
Leu Asn Ile Glu Gln Arg Leu Lys Glu Ala Thr Glu Trp Leu His Tyr645 650 655Ile Phe Asn Pro Leu Glu Asp Glu Leu Val Gln Ala Ser Asn Gln Gly660 665 670Lys Pro Arg Tyr Trp Asn Ser Arg Pro Ile Ile Asp Pro Pro Pro Thr675 680 685Val Tyr Arg Met Leu Ile Glu Pro Thr Asp Pro Asp Ala Ile Ala Ala690 695 700Ser Glu Pro Ile His Tyr Arg Lys Ala Ile Phe Arg Phe Tyr Val Lys705 710 715 720Asn Leu Leu Asp Gln Gly Asp Met Glu Tyr Arg Lys Leu Thr Ser Ser725 730 735Ala Arg Thr Val Ala Lys Gln Ile Tyr Asp Ser Val Asn Met Leu Leu740 745 750Gly Thr Ser Pro Asp Ile Leu Leu Ala Ala Asn Trp Gln Pro Arg Thr755 760 765Leu Gln Asp Val Ala Leu Tyr Glu Asn Ser Glu Ala Arg Ala Gln Glu770 775 780Leu Met Leu Thr Val Ser Ser Val Pro Leu Leu Pro Val Thr Tyr Asp785 790 795 800Thr Ser Val Ser Ala Ala Pro Ser Asp Leu Phe Val Lys Pro Val Asp805 810 815
Thr Glu Tyr Leu Lys Leu Trp Gln Met Leu Asp Gln Arg Leu Tyr Asn820 825 830Leu Arg His Asn Leu Thr Leu Asp Gly Lys Glu Phe Pro Ala Gly Leu835 840 845Tyr Asp Glu Pro Ile Ser Pro Gln Asp Leu Leu Arg Gln Arg Tyr Gln850 855 860Arg Val Val Ala Asn Arg Met Ala Gly Met Lys Arg Arg Ala Ile Pro865 870 875 880Asn Tyr Arg Phe Thr Pro Ile Met Ser Arg Ala Lys Glu Ala Ala Glu885 890 895Thr Leu Ile Gln Tyr Gly Ser Thr Leu Leu Ser Leu Leu Glu Lys Lys900 905 910Asp Asn Thr Asp Phe Glu His Phe Arg Met Gln Gln Gln Leu Gly Leu915 920 925Tyr Ser Phe Thr Arg Asn Leu Gln Gln Gln Ala Ile Asp Met Gln Gln930 935 940Ala Ser Leu Asp Ala Leu Thr Ile Ser Arg Arg Ala Ala Gln Glu Arg945 950 955 960Gln Gln His Tyr Lys Ser Leu Tyr Asp Glu Asn Ile Ser Ile Thr Glu965 970 975Gln Glu Val Ile Ala Leu Gln Ser Arg Ala Ala Glu Gly Val Ile Ala980 985 990
Ala Gln Ser Ala Ala Thr Ala Ala Ala Val Ala Asp Met Val Pro Asn995 1000 1005Ile Phe Gly Leu Ala Val Gly Gly Met Val Phe Gly Gly Met Leu1010 1015 1020Arg Ala Ile Gly Glu Gly Ile Arg Ile Asp Val Glu Ser Lys Asn1025 1030 1035Ala Lys Ala Thr Ser Leu Ser Val Ser Glu Asn Tyr Arg Arg Arg1040 1045 1050Gln Gln Glu Trp Glu Leu Gln Tyr Lys Gln Ala Asp Ile Asn Ile1055 1060 1065Glu Glu Ile Asp Ala Gln Ile Gly Ile Gln Gln Arg Gln Leu Asn1070 1075 1080Ile Ser Thr Thr Gln Leu Ala Gln Leu Glu Ala Gln His Glu Gln1085 1090 1095Asp Gln Val Leu Leu Glu Tyr Tyr Ser Asn Arg Phe Thr Asn Asp1100 1105 1110Ala Leu Tyr Met Trp Met Ile Ser Gln Ile Ser Gly Leu Tyr Leu1115 1120 1125Gln Ala Tyr Asp Ala Val Asn Ser Leu Cys Leu Leu Ala Glu Ala1130 1135 1140Ser Trp Gln Tyr Glu Thr Gly Gln Tyr Asp Met Asn Phe Val Gln1145 1150 1155
Ser Gly Leu Trp Asn Asp Leu Tyr Gln Gly Leu Leu Val Gly Glu1160 1165 1170His Leu Lys Leu Ala Leu Gln Arg Met Asp Gln Ala Tyr Leu Gln1175 1180 1185His Asn Thr Arg Arg Leu Glu Ile Ile Lys Thr Ile Ser Val Lys1190 1195 1200Ser Leu Leu Thr Ser Ser Gln Trp Glu Ile Gly Lys Ser Thr Gly1205 1210 1215Ser Phe Thr Phe Leu Leu Ser Ala Glu Met Phe Leu Arg Asp Tyr1220 1225 1230Pro Thr His Ala Asp Arg Arg Ile Lys Thr Val Ala Leu Ser Leu1235 1240 1245Pro Ala Leu Leu Gly Pro Tyr Glu Asp Val Arg Ala Ser Leu Val1250 1255 1260Gln Leu Ser Asn Thr Leu Tyr Ser Thr Ala Asp Leu Lys Thr Ile1265 1270 1275Asp Tyr Leu Leu Asn Pro Leu Glu Tyr Thr Lys Pro Glu Asn Val1280 1285 1290Leu Leu Asn Val Gln Ala Asn Gln Gly Val Val Ile Ser Thr Ala1295 1300 1305Met Glu Asp Ser Gly Met Phe Arg Leu Asn Phe Asp Asp Glu Leu1310 1315 1320
Phe Leu Pro Phe Glu Gly Thr Gly Ala Ile Ser Gln Trp Lys Leu1325 1330 1335Glu Phe Gly Ser Asp Gln Asp Gln Leu Leu Glu Ser Leu Ser Asp1340 1345 1350Ile Ile Leu His Leu Arg Tyr Thr Ala Arg Asp Val Ser Gly Gly1355 1360 1365Ser Asn Glu Phe Ser Gln Gln Val Arg Ser Arg Leu Asn Lys His1370 1375 1380Gln Leu Lys Gln Asp Asn Ser Asn1385 1390<210>11<211>1944<212>DNA<213>嗜线虫致病杆菌<400>11atgtctcaaa atgtttatcg atacccttca attaaagcga tgtctgacgc cagcagcgaa 60gtaggcgcat ctctggttgc ctggcagaat caatctggtg gtcaaacctg gtatgtcatt120tatgatagcg cggtttttaa aaacatcggc tgggttgaac gctggcatat tcccgaccgc180aatatttcac ctgatttacc ggtttatgag aatgcctggc aatatgtccg tgaggcgaca240ccggaagaaa ttgccgatca cggtaacccc aatacgcctg atgtaccgcc gggagaaaaa300accgaggtat tgcaatatga tgcactcaca gaagaaacct atcagaaggt gggatataaa360cctgacggca gcggaactcc tttgagttat tcttcagcac gtgttgccaa gtccctgtac420aacgaatatg aagttgatcc ggaaaataca gaaccgctgc ctaaagtctc tgcctatatt480actgactggt gccagtatga tgcgcgtttg tcgccagaaa cccaggataa cactgcgctg540
accagcgacg atgcccccgg ccgtggtttt gatctggaaa aaatcccgcc taccgcctac 600gaccgcctga ttttcagttt tatggccgtc aacggtgata aaggcaagtt atccgaacgg 660attaatgagg ttgttgacgg gtggaaccgg caagcagaag ccagcagtgg ccagattgcc 720cctattacat taggccatat tgtacccgtt gatccttatg gtgatttagg caccacacgc 780aatgtcggtc tggacgcgga tcagcgccgt gatgccagcc cgaagaattt cttgcaatat 840tacaatcagg atgcagcctc cggtttactg gggggattgc gtaatctgaa agcgcgagca 900aaacaggcag ggcacaagct ggaactcgca ttcagtatcg gcggctggag tatgtcaggg 960tatttctctg tgatggccaa agatcctgag caacgtgcta catttgtgag tagcatcgtc1020gacttcttcc ggcgttttcc catgtttact gcggtggata tcgactggga ataccccggc1080gccacaggtg aagaaggtaa tgaattcgac ccggaacatg atggcccaaa ctatgttttg1140ttagtgaaag agctgcgtga agcactgaac atcgcctttg gaacccgggc ccgtaaagaa1200atcacgatag cctgtagcgc cgtcgttgcc aaaatggaga agtccagctt caaagaaatc1260gcaccttatt tagacaatat ctttgtgatg acctacgact tctttggtac cggttgggca1320gaatacatcg gtcaccatac taacctgtat ccccccagat atgaatatga cggcgataac1380cctcctccgc ccaatcctga tcgggacatg gattactcgg ctgatgaggc gatccgcttt1440ttactgtcac aaggtgtaca accggagaaa attcacctcg gatttgctaa ctatggacgt1500tcatgtctgg gtgctgatct gacaactcgc cgctataaca gaacaggaga gccactgggc1560acgatggaaa aaggtgctcc ggaattcttc tgtctgctga ataaccaata cgatgcggaa1620tatgaaattg cacgcgggaa aaatcagttt gaactggtga cagacacgga aaccgacgct1680gacgcactct ttaatgctga cggtggtcac tggatttcac tggatacgcc ccgcactgtg1740ctgcataagg gaatttatgc aaccaaaatg aaattgggcg ggatcttctc ttggtcaggc1800gatcaggatg atggcctgtt ggcaaatgct gctcacgaag gtttgggtta cttacctgta1860
cgcggaaaag agaagattga tatgggaccg ttatataaca aaggacgtct cattcagctt 1920cctaaagtaa cccgtcgtaa atcg 1944<210>12<211>648<212>PRT<213>嗜线虫致病杆菌<400>12Met Ser Gln Asn Val Tyr Arg Tyr Pro Ser Ile Lys Ala Met Ser Asp1 5 10 15Ala Ser Ser Glu Val Gly Ala Ser Leu Val Ala Trp Gln Asn Gln Ser20 25 30Gly Gly Gln Thr Trp Tyr Val Ile Tyr Asp Ser Ala Val Phe Lys Asn35 40 45Ile Gly Trp Val Glu Arg Trp His Ile Pro Asp Arg Asn Ile Ser Pro50 55 60Asp Leu Pro Val Tyr Glu Asn Ala Trp Gln Tyr Val Arg Glu Ala Thr65 70 75 80Pro Glu Glu Ile Ala Asp His Gly Asn Pro Asn Thr Pro Asp Val Pro85 90 95Pro Gly Glu Lys Thr Glu Val Leu Gln Tyr Asp Ala Leu Thr Glu Glu100 105 110Thr Tyr Gln Lys Val Gly Tyr Lys Pro Asp Gly Ser Gly Thr Pro Leu115 120 125
Ser Tyr Ser Ser Ala Arg Val Ala Lys Ser Leu Tyr Asn Glu Tyr Glu130 135 140Val Asp Pro Glu Asn Thr Glu Pro Leu Pro Lys Val Ser Ala Tyr Ile145 150 155 160Thr Asp Trp Cys Gln Tyr Asp Ala Arg Leu Ser Pro Glu Thr Gln Asp165 170 175Asn Thr Ala Leu Thr Ser Asp Asp Ala Pro Gly Arg Gly Phe Asp Leu180 185 190Glu Lys Ile Pro Pro Thr Ala Tyr Asp Arg Leu Ile Phe Ser Phe Met195 200 205Ala Val Asn Gly Asp Lys Gly Lys Leu Ser Glu Arg Ile Asn Glu Val210 215220Val Asp Gly Trp Asn Arg Gln Ala Glu Ala Ser Ser Gly Gln Ile Ala225 230 235 240Pro Ile Thr Leu Gly His Ile Val Pro Val Asp Pro Tyr Gly Asp Leu245 250 255Gly Thr Thr Arg Asn Val Gly Leu Asp Ala Asp Gln Arg Arg Asp Ala260 265 270Ser Pro Lys Asn Phe Leu Gln Tyr Tyr Asn Gln Asp Ala Ala Ser Gly275 280 285Leu Leu Gly Gly Leu Arg Asn Leu Lys Ala Arg Ala Lys Gln Ala Gly290 295 300
His Lys Leu Glu Leu Ala Phe Ser Ile Gly Gly Trp Ser Met Ser Gly305 310 315 320Tyr Phe Ser Val Met Ala Lys Asp Pro Glu Gln Arg Ala Thr Phe Val325 330 335Ser SerIle Val Asp Phe Phe Arg Arg Phe Pro Met Phe Thr Ala Val340 345 350Asp Ile Asp Trp Glu Tyr Pro Gly Ala Thr Gly Glu Glu Gly Asn Glu355 360 365Phe Asp Pro Glu His Asp Gly Pro Asn Tyr Val Leu Leu Val Lys Glu370 375 380Leu Arg Glu Ala Leu Asn Ile Ala Phe Gly Thr Arg Ala Arg Lys Glu385 390 395 400Ile Thr Ile Ala Cys Ser Ala Val Val Ala Lys Met Glu Lys Ser Ser405 410 415Phe Lys Glu Ile Ala Pro Tyr Leu Asp Asn Ile Phe Val Met Thr Tyr420 425 430Asp Phe Phe Gly Thr Gly Trp Ala Glu Tyr Ile Gly His His Thr Asn435 440 445Leu Tyr Pro Pro Arg Tyr Glu Tyr Asp Gly Asp Asn Pro Pro Pro Pro450 455 460Asn Pro Asp Arg Asp Met Asp Tyr Ser Ala Asp Glu Ala Ile Arg Phe465 470 475 480
Leu Leu Ser Gln Gly Val Gln Pro Glu Lys Ile His Leu Gly Phe Ala485 490 495Asn Tyr Gly Arg Ser Cys Leu Gly Ala Asp Leu Thr Thr Arg Arg Tyr500 505 510Asn Arg Thr Gly Glu Pro Leu Gly Thr Met Glu Lys Gly Ala Pro Glu515 520 525Phe Phe Cys Leu Leu Asn Asn Gln Tyr Asp Ala Glu Tyr Glu Ile Ala530 535 540Arg Gly Lys Asn Gln Phe Glu Leu Val Thr Asp Thr Glu Thr Asp Ala545 550 555 560Asp Ala Leu Phe Asn Ala Asp Gly Gly His Trp Ile Ser Leu Asp Thr565 570 575Pro Arg Thr Val Leu His Lys Gly Ile Tyr Ala Thr Lys Met Lys Leu580 585 590Gly Gly Ile Phe Ser Trp Ser Gly Asp Gln Asp Asp Gly Leu Leu Ala595 600 605Asn Ala Ala His Glu Gly Leu Gly Tyr Leu Pro Val Arg Gly Lys Glu610 615 620Lys Ile Asp Met Gly Pro Leu Tyr Asn Lys Gly Arg Leu Ile Gln Leu625 630 635 640Pro Lys Val Thr Arg Arg Lys Ser645
<210>13<211>7569<212>DNA<213>嗜线虫致病杆菌<400>13atgataaaag ttaatgaact gttagataag ataaatagaa aaaggtctgg tgatacttta 60ttattgacaa acatttcgtt tatgtctttc agcgaatttc gtcataggac aagtggaact 120ctgacgtggc gagaaacaga ctttttatat caacaggctc atcaggaatc aaaacagaat 180aaacttgaag aactgcgcat tttgtcccgt gctaatccac aactggctaa taccactaac 240cttaatatta caccgtcaac cctaaacaat agttacaaca gttggtttta tggccgtgcc 300caccgttttg taaaaccggg atcaattgct tccatatttt caccagcggc ttatttaaca 360gaattatatc gggaagcgaa agattttcat cctgacaatt ctcaatatca cctgaataaa 420cgacgccccg acattgcttc actggcactg acacagaata atatggatga agaaatttcc 480acattatcct tatctaatga attactgctg cataatattc agacgttaga gaaaactgac 540tataacggtg taatgaaaat gttgtccact taccggcaaa ccggcatgac accctatcat 600ctgccgtatg agtcagcccg tcaggcaatt ttattgcaag ataaaaacct caccgcattt 660agccgtaata cagacgtagc ggaattaatg gacccaacat cgctactggc tattaagact 720gatatatcgc ctgaattgta tcaaatcctt gtagaagaaa ttacaccgga aaattcaaca 780gaactgatga agaaaaattt cggtacagat gatgtactga tttttaagag ttatgcttct 840ttggctcgct actacgattt gtcttatgat gaactcagtt tatttgtcaa tctctccttc 900ggtaagaaaa atacaaatca acagtataag aatgagcaac tgataacatt ggtcaatgac 960gggaatgata cggcaacggc aagattgatt aagcgaaccc gcaaagattt ctacgattca1020catttaaact atgcagaact aattccaatc aaagaaaatg aatacaaata taatttcagt1080gtaaaaaaaa cagaacctga ccacttggat tttcgtctcc agaatggaga taaagaatat1140
atataccaag ataaaaattt cgtccccatt gctaataccc attacagtat tcccattaaa1200ttgacgacag agcaaatcac caacggtata acactccgct tatggcgagt taaaccaaat1260ccgtcggatg ctatcaatgc caatgcatac tttaaaatga tggagttccc cggtgatata1320ttcctgttaa agctgaataa agcgattcgt ttgtataaag ccacaggcat atctccagaa1380gatatctggc aagtaataga aagtatttat gatgacttaa ccattgacag caatgtgttg1440ggtaagctgt tttatgttca atattatatg cagcactata atattagcgt cagcgatgcg1500ctggtattgt gtcattcaga tatcagccaa tattccacta aacaacaacc cagtcatttt1560acaatactgt tcaatacacc gctattaaat ggccaagagt tttctgctga taataccaaa1620ctggatttaa cccccggtga atcaaaaaac catttttatt tgggaataat gaaacgtgct1680ttcagagtga atgatactga actgtataca ttatggaagc tggctaatgg cggaacaaat1740ccagaattta tgtgttccat cgagaacctg tctctgcttt atcgcgttcg tctgctggca1800gacattcatc atctgacagt gaatgaatta tccatgttgt tgtcggtttc tccctatgtg1860aacacgaaaa ttgccctttt ttctgataca gcattaacgc aattaatcag ctttctgttc1920caatgcaccc agtggctgac aacacagaaa tggtctgtca gtgatgtgtt tctgatgacc1980acggataatt acagcactgt ccttacgccg gatattgaaa accttatcac gacactaagt2040aatggattat caacactttc actcggtgat gacgaactga tccgtgcagc tgccccgctg2100attgctgcca gcattcaaat ggattcagcc aagacagcag aaactatttt gctgtggatt2160aatcagataa aaccacaagg actgacattc gatgatttca tgattattgc ggctaaccgt2220gatcgctcag agaatgaaac cagcaacatg gtggcttttt gtcaggtact ggggcaactt2280tctctgattg tgcgcaatat tggactcagc gaaaacgaac tgaccctgtt ggtgacaaaa2340ccggagaaat tccaatcaga aaccacagca ctgcaacatg atctccccac tttgcaagcg2400ctgacccgct tccatgctgt gatcatgcgt tgtggaagct acgcgacaga aatcttaaca2460
gcattggaac taggagcgct gactgccgaa caattggcgg tggcgttaaa atttgatgct2520caggttgtga cacaagcatt gcaacagacc ggtttgggag tgaatacctt taccaactgg2580agaactatag atgtcactct gcaatggctg gatgtcgctg ctacattggg tattaccccg2640gatggtgttg ctgcactcat aaaattaaaa tatatcggtg aaccagaaac cccgatgcca2700acatttgatg attggcaagc cgccagtact ttgttgcagg cgggactgaa cagtcaacaa2760tccgaccagc ttcaggcatg gctggatgaa gccacgacga cagcggccag tgcttactac2820atcaaaaata gtgcacctca acagattaag agccgggatg agttgtacag ctatctgctg2880attgataacc aagtttctgc ccaagtgaaa accacccgtg tggcagaagc cattgccagc2940attcagttat atgtcaaccg ggcgttgaat aatgttgaag gaaaagtatc aaagccagtg3000aaaacccgtc agttcttctg cgactgggaa acctacaatc gacggtatag cacctgggcc3060ggcgtatctg aactggccta ttatccggaa aactatatcg accccacgat tcgtattggt3120cagacaggta tgatgaacaa cctgttacag caactttccc aaagtcagtt aaatatcgat3180accgttgaag atagctttaa aaattatctg accgcatttg aagatgtcgc taacttgcag3240gtgattagcg gatatcatga cagtatcaat gtcaatgagg gactcactta tttaattggt3300tatagccaga cagaacccag aatatattat tggcgcaatg tcgatcacca aaagtgccag3360cacggtcaat ttgctgccaa tgcctgggga gaatggaaaa aaattgaaat acccatcaat3420gtatggcagg aaaatatcag acctgttatt tacaagtctc gtttgtattt actgtggctg3480gaacaaaaag agctgaaaaa tgaaagtgaa gatggcaaga tagatatcac tgattatata3540ttaaaactgt cacatattcg ttatgatggc agctggagct caccgtttaa ttttaatgtg3600actgataaaa tagaaaacct gatcaataaa aaagccagca ttggtatgta ttgttcttct3660gattatgaaa aagacgtcat tattgtttat ttccatgaga aaaaagacaa ttattctttt3720aatagtcttc ctgcaagaga agggatgacc attaaccctg atatgacatt atccattctc3780
acagaaaatg atttagacgc cattgttaag agcacattat cagaacttga taccaggaca3840gaatacaaag tcaacaatca atttgctaca gattatttgg ccgaatataa ggaatctata3900accacaaaaa ataaattagc cagttttacc ggaaatattt ttgatctctc gtatatatca3960ccaggaaatg gtcatattaa tttaacgttc aatccttcaa tggaaattaa tttttcaaaa4020ggcaatatat ataatgatga ggttaaatac ctgttatcga tggtagaaga tgaaacggtt4080attttatttg attatgatag acatgatgaa atgcttggaa aagaagaaga agtttttcat4140tatggaactt tggattttat tatttccatc gatcttaaaa atgccgaata ttttagagtg4200ttaatgcatc taagaaccaa ggaaaaaatt cctagaaaat cagaaattgg agttggtata4260aattatgatt atgaatcaaa tgatgctgaa ttcaaacttg atactaacat agtattagat4320tggaaagata acacaggagt atggcatact atatgtgaat catttactaa tgatgtttca4380atcattaata acatgggaaa tattgcggca ctgttccttc gcgaggatcc atgtgtgtat4440ttatgttcaa tagccacaga tataaaaatt gcttcatcta tgatcgaaca gatccaagat4500aaaaacatta gttttttatt aaaaaatggc tctgatattc tagtggagtt aaatgctgaa4560gaccatgtgg catctaaacc ttcacacgaa tctgacccta tggtatatga ttttaatcaa4620gtaaaagttg atattgaagg ctatgatatt cctctggtga gcgagtttat tattaagcaa4680cccgacggcg gttataacga tattgttatt gaatcgccaa ttcatataaa actaaaatcc4740aaagatacaa gtaacgttat atcactgcat aaaatgccat caggcacaca atatatgcag4800attggccctt acagaacccg gttaaatact ttattttcca gaaaattagc tgaaagagcc4860aatattggta ttgataatgt tttaagtatg gaaacgcaaa atttaccaga gccgcaatta4920ggtgaagggt tttatgcgac atttaagttg cccccctaca ataaagagga gcatggtgat4980gaacgttggt ttaagatcca tattgggaat attgatggca attctgccag acaaccttat5040tacgaaggaa tgttatctga tattgaaacc acagtaacgc tctttgttcc ctatgctaaa5100
ggatattaca tacgtgaagg tgtcagatta ggggttgggt acaaaaaaat tatctatgac5160aaatcctggg aatctgcttt cttttatttt gatgagacga aaaatcaatt tatattcatt5220aatgatgccg atcatgattc gggaatgaca caacagggga tagtaaaaaa tatcaaaaaa5280tataaagggt ttattcatgt cgttgtcatg aaaaataaca ctgaacccat ggatttcaac5340ggcgccaatg caatctattt ctgggaattg ttctattaca cgcccatgat ggtattccag5400cgcttattgc aagagcagaa ttttaccgaa tcgacacgct ggctgcgcta tatctggaac5460ccggccggat attcggttca gggtgaaatg caggattatt actggaacgt ccgcccattg5520gaggaagata cgtcctggaa tgccaatccg ctggattcgg tcgatcctga cgccgttgcc5580cagcatgatc cgatgcacta taaagtggct acctttatga aaatgctgga tttgttgatt5640acccgcggag atagcgccta tcgccagctt gaacgtgata ccttaaacga agctaaaatg5700tggtatgtac aggcgctcac tttattgggt gatgagcctt atttttcatt ggataacgat5760tggtcagagc cacggctgga agaagctgcc agccaaacaa tgcggcatca ttatcaacat5820aaaatgctgc aactgcgtca gcgcgctgca ttacccacga aacgtacggc aaattcgtta5880accgcattgt tcctccctca aattaataaa aaactgcaag gttactggca gacattgacg5940caacgcctct ataacttacg ccataacctg acaatcgacg gtcagccact gtcattatct6000ctctatgcca cgcccgcaga tccgtccatg ttactcagtg ctgccatcac tgcttcacaa6060ggcggcggcg atttacctca tgcagtgatg ccgatgtacc gttttccggt gattctggaa6120aatgccaagt ggggggtaag ccagttgata caatttggca ataccctgct cagcattact6180gaacggcagg atgcagaagc cttggctgaa atactgcaaa ctcaaggcag tgagttagcc6240ctgcaaagta ttaaaatgca ggataaggtc atggctgaaa ttgatgctga taaattggcg6300cttcaagaaa gccgtcatgg tgcacagtct cgttttgaca gtttcaatac gctgtacgac6360gaagatgtta acgctggtga aaaacaagcg atggatcttt acctctcttc atcggtcttg6420
agcaccagcg gcacagccct gcatatggcc gccgccgcgg cagatctcgt ccccaatatt6480tacggttttg ctgtgggagg ttcccgtttt ggggcgcttt tcaatgccag tgcgattggt6540atcgaaattt ctgcgtcagc aacacgtatt gccgcagaca aaatcagcca atcagaaata6600taccgtcgcc gtcggcaaga gtgggaaatt cagcgcaata atgcggaagc tgagataaaa6660caaattgatg ctcaattagc gacgctggct gtacgtcgtg aagcggcagt attacaaaaa6720aactatctgg aaactcagca ggcacaaact caggcgcagt tagcctttct gcaaagtaaa6780ttcagtaatg cagcgctata caactggctc cgtggaaggt tgtccgctat ttattatcag6840ttttatgatt tggcggtctc actctgttta atggcagagc aaacttatca gtatgaattg6900aataatgcgg cagcacactt tattaaacca ggtgcctggc atgggactta tgcgggttta6960ttagcgggtg aaaccctgat gctgaattta gcacagatgg aaaaaagcta tttggaaaaa7020gatgaacggg cactggaggt caccagaacc gtttctctgg ctgaagtgta tgctggtctg7080acagaaaata gtttcatttt aaaagataaa gtgactgagt tagtcaatgc aggtgaaggc7140agtgcaggca caacgcttaa cggtttgaac gtcgaaggga cacaactgca agccagcctc7200aaattatcgg atctgaatat tgctaccgat tatcctgacg gtttaggtaa tacacgccgt7260atcaaacaaa tcagtgtgac attacctgcc cttttagggc cttatcagga tgttcgggca7320atactaagtt atggcggcag cacaatgatg ccacgtggct gcaaagcgat tgcgatctca7380catggcatga atgacagtgg tcaattccag atggatttca atgatgccaa gtacctgcca7440tttgaagggc ttcctgtggc cgatacaggc acattaaccc tcagttttcc cggtatcagt7500ggtaaacaga aaagcttatt gctcagcctg agcgatatca ttctgcatat ccgttacacc7560attcgttct7569<210>14<211>2523<212>PRT
<213>嗜线虫致病杆菌<400>14Met Ile Lys Val Asn Glu Leu Leu Asp Lys Ile Asn Arg Lys Arg Ser1 5 10 15Gly Asp Thr Leu Leu Leu Thr Asn Ile Ser Phe Met Set Phe Ser Glu20 25 30Phe Arg His Arg Thr Ser Gly Thr Leu Thr Trp Arg Glu Thr Asp Phe35 40 45Leu Tyr Gln Gln Ala His Gln Glu Ser Lys Gln Asn Lys Leu Glu Glu50 55 60Leu Arg Ile Leu Ser Arg Ala Asn Pro Gln Leu Ala Asn Thr Thr Asn65 70 75 80Leu Asn Ile Thr Pro Ser Thr Leu Asn Asn Ser Tyr Asn Ser Trp Phe85 90 95Tyr Gly Arg Ala His Arg Phe Val Lys Pro Gly Ser Ile Ala Ser Ile100 105 110Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asp115 120 125Phe His Pro Asp Asn Ser Gln Tyr His Leu Asn Lys Arg Arg Pro Asp130 135 140Ile Ala Ser Leu Ala Leu Thr Gln Asn Asn Met Asp Glu Glu Ile Ser145 150 155 160
Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu His Asn Ile Gln Thr Leu165 170 175Glu Lys Thr Asp Tyr Asn Gly Val Met Lys Met Leu Ser Thr Tyr Arg180 185 190Gln Thr Gly Met Thr Pro Tyr His Leu Pro Tyr Glu Ser Ala Arg Gln195 200 205Ala Ile Leu Leu Gln Asp Lys Asn Leu Thr Ala Phe Ser Arg Asn Thr210 215 220Asp Val Ala Glu Leu Met Asp Pro Thr Ser Leu Leu Ala Ile Lys Thr225 230 235 240Asp Ile Ser Pro Glu Leu Tyr Gln Ile Leu Val Glu Glu Ile Thr Pro245 250 255Glu Asn Ser Thr Glu Leu Met Lys Lys Asn Phe Gly Thr Asp Asp Val260 265 270Leu Ile Phe Lys Ser Tyr Ala Ser Leu Ala Arg Tyr Tyr Asp Leu Ser275 280 285Tyr Asp Glu Leu Ser Leu Phe Val Asn Leu Ser Phe Gly Lys Lys Asn290 295 300Thr Asn Gln Gln Tyr Lys Asn Glu Gln Leu Ile Thr Leu Val Asn Asp305 310 315 320Gly Asn Asp Thr Ala Thr Ala Arg Leu Ile Lys Arg Thr Arg Lys Asp325 330 335
Phe Tyr Asp Ser His Leu Asn Tyr Ala Glu Leu Ile Pro Ile Lys Glu340 345 350Asn Glu Tyr Lys Tyr Asn Phe Ser Val Lys Lys Thr Glu Pro Asp His355 360 365Leu Asp Phe Arg Leu Gln Asn Gly Asp Lys Glu Tyr Ile Tyr Gln Asp370 375 380Lys Asn Phe Val Pro Ile Ala Asn Thr His Tyr Ser Ile Pro Ile Lys385 390 395 400Leu Thr Thr Glu Gln Ile Thr Asn Gly Ile Thr Leu Arg Leu Trp Arg405 410 415Val Lys Pro Asn Pro Ser Asp Ala Ile Asn Ala Asn Ala Tyr Phe Lys420 425 430Met Met Glu Phe Pro Gly Asp Ile Phe Leu Leu Lys Leu Asn Lys Ala435 440 445Ile Arg Leu Tyr Lys Ala Thr Gly Ile Ser Pro Glu Asp Ile Trp Gln450 455 460Val Ile Glu Ser Ile Tyr Asp Asp Leu Thr Ile Asp Ser Asn Val Leu465 470 475 480Gly Lys Leu Phe Tyr Val Gln Tyr Tyr Met Gln His Tyr Asn Ile Ser485 490 495Val Ser Asp Ala Leu Val Leu Cys His Ser Asp Ile Ser Gln Tyr Ser500 505 510
Thr Lys Gln Gln Pro Ser His Phe Thr Ile Leu Phe Asn Thr Pro Leu515 520 525Leu Asn Gly Gln Glu Phe Ser Ala Asp Asn Thr Lys Leu Asp Leu Thr530 535 540Pro Gly Glu Ser Lys Asn His Phe Tyr Leu Gly Ile Met Lys Arg Ala545 550 555 560Phe Arg Val Asn Asp Thr Glu Leu Tyr Thr Leu Trp Lys Leu Ala Asn565 570 575Gly Gly Thr Asn Pro Glu Phe Met Cys Ser Ile Glu Asn Leu Ser Leu580 585 590Leu Tyr Arg Val Arg Leu Leu Ala Asp Ile His His Leu Thr Val Asn595 600 605Glu Leu Ser Met Leu Leu Ser Val Ser Pro Tyr Val Asn Thr Lys Ile610 615 620Ala Leu Phe Ser Asp Thr Ala Leu Thr Gln Leu Ile Ser Phe Leu Phe625 630 635 640Gln Cys Thr Gln Trp Leu Thr Thr Gln Lys Trp Ser Val Ser Asp Val645 650 655Phe Leu Met Thr Thr Asp Asn Tyr Ser Thr Val Leu Thr Pro Asp Ile660 665 670Glu Asn Leu Ile Thr Thr Leu Ser Asn Gly Leu Ser Thr Leu Ser Leu675 680 685
Gly Asp Asp Glu Leu Ile Arg Ala Ala Ala Pro Leu Ile Ala Ala Ser690 695 700Ile Gln Met Asp Ser Ala Lys Thr Ala Glu Thr Ile Leu Leu Trp Ile705 710 715 720Asn Gln Ile Lys Pro Gln Gly Leu Thr Phe Asp Asp Phe Met Ile Ile725 730 735Ala Ala Asn Arg Asp Arg Ser Glu Asn Glu Thr Ser Asn Met Val Ala740 745 750Phe Cys Gln Val Leu Gly Gln Leu Ser Leu Ile Val Arg Asn Ile Gly755 760 765Leu Ser Glu Asn Glu Leu Thr Leu Leu Val Thr Lys Pro Glu Lys Phe770 775 780Gln Ser Glu Thr Thr Ala Leu Gln His Asp Leu Pro Thr Leu Gln Ala785 790 795 800Leu Thr Arg Phe His Ala Val Ile Met Arg Cys Gly Ser Tyr Ala Thr805 810 815Glu Ile Leu Thr Ala Leu Glu Leu Gly Ala Leu Thr Ala Glu Gln Leu820 825 830Ala Val Ala Leu Lys Phe Asp Ala Gln Val Val Thr Gln Ala Leu Gln835 840 845Gln Thr Gly Leu Gly Val Asn Thr Phe Thr Asn Trp Arg Thr Ile Asp850 855 860
Val Thr Leu Gln Trp Leu Asp Val Ala Ala Thr Leu Gly Ile Thr Pro865 870 875 880Asp Gly Val Ala Ala Leu Ile Lys Leu Lys Tyr Ile Gly Glu Pro Glu885 890 895Thr Pro Met Pro Thr Phe Asp Asp Trp Gln Ala Ala Ser Thr Leu Leu900 905 910Gln Ala Gly Leu Asn Ser Gln Gln Ser Asp Gln Leu Gln Ala Trp Leu915 920 925Asp Glu Ala Thr Thr Thr Ala Ala Ser Ala Tyr Tyr Ile Lys Asn Ser930 935 940Ala Pro Gln Gln Ile Lys Ser Arg Asp Glu Leu Tyr Ser Tyr Leu Leu945 950 955 960Ile Asp Asn Gln Val Ser Ala Gln Val Lys Thr Thr Arg Val Ala Glu965 970 975Ala Ile Ala Ser Ile Gln Leu Tyr Val Asn Arg Ala Leu Asn Asn Val980 985 990Glu Gly Lys Val Ser Lys Pro Val Lys Thr Arg Gln Phe Phe Cys Asp995 1000 1005Trp Glu Thr Tyr Asn Arg Arg Tyr Ser Thr Trp Ala Gly Val Ser1010 1015 1020Glu Leu Ala Tyr Tyr Pro Glu Asn Tyr Ile Asp Pro Thr Ile Arg1025 1030 1035
Ile Gly Gln Thr Gly Met Met Asn Asn Leu Leu Gln Gln Leu Ser1040 1045 1050Gln Ser Gln Leu Asn Ile Asp Thr Val Glu Asp Ser Phe Lys Asn1055 1060 1065Tyr Leu Thr Ala Phe Glu Asp Val Ala Asn Leu Gln Val Ile Ser1070 1075 1080Gly Tyr His Asp Ser Ile Asn Val Asn Glu Gly Leu Thr Tyr Leu1085 1090 1095Ile Gly Tyr Ser Gln Thr Glu Pro Arg Ile Tyr Tyr Trp Arg Asn1100 1105 1110Val Asp His Gln Lys Cys Gln His Gly Gln Phe Ala Ala Asn Ala1115 1120 1125Trp Gly Glu Trp Lys Lys Ile Glu Ile Pro Ile Asn Val Trp Gln1130 1135 1140Glu Asn Ile Arg Pro Val Ile Tyr Lys Ser Arg Leu Tyr Leu Leu1145 1150 1155Trp Leu Glu Gln Lys Glu Leu Lys Asn Glu Ser Glu Asp Gly Lys1160 1165 1170Ile Asp Ile Thr Asp Tyr Ile Leu Lys Leu Ser His Ile Arg Tyr1175 1180 1185Asp Gly Ser Trp Ser Ser Pro Phe Asn Phe Asn Val Thr Asp Lys1190 1195 1200
Ile Glu Asn Leu Ile Asn Lys Lys Ala Ser Ile Gly Met Tyr Cys1205 1210 1215Ser Ser Asp Tyr Glu Lys Asp Val Ile Ile Val Tyr Phe His Glu1220 1225 1230Lys Lys Asp Asn Tyr Ser Phe Asn Ser Leu Pro Ala Arg Glu Gly1235 1240 1245Met Thr Ile Asn Pro Asp Met Thr Leu Ser Ile Leu Thr Glu Asn1250 1255 1260Asp Leu Asp Ala Ile Val Lys Ser Thr Leu Ser Glu Leu Asp Thr1265 1270 1275Arg Thr Glu Tyr Lys Val Asn Asn Gln Phe Ala Thr Asp Tyr Leu1280 1285 1290Ala Glu Tyr Lys Glu Ser Ile Thr Thr Lys Asn Lys Leu Ala Ser1295 1300 1305Phe Thr Gly Asn Ile Phe Asp Leu Ser Tyr Ile Ser Pro Gly Asn1310 1315 1320Gly His Ile Asn Leu Thr Phe Asn Pro Ser Met Glu Ile Asn Phe1325 1330 1335Ser Lys Gly Asn Ile Tyr Asn Asp Glu Val Lys Tyr Leu Leu Ser1340 1345 1350Met Val Glu Asp Glu Thr Val Ile Leu Phe Asp Tyr Asp Arg His1355 1360 1365
Asp Glu Met Leu Gly Lys Glu Glu Glu Val Phe His Tyr Gly Thr1370 1375 1380Leu Asp Phe IleIle Ser Ile Asp Leu Lys Asn Ala Glu Tyr Phe1385 1390 1395Arg Val Leu Met His Leu Arg Thr Lys Glu Lys Ile Pro Arg Lys1400 1405 1410Ser Glu Ile Gly Val Gly Ile Asn Tyr Asp Tyr Glu Ser Asn Asp1415 1420 1425Ala Glu Phe Lys Leu Asp Thr AsnIle Val Leu Asp Trp Lys Asp1430 1435 1440Asn Thr Gly Val Trp His Thr Ile Cys Glu Ser Phe Thr Asn Asp1445 1450 1455Val Ser Ile Ile Asn Asn Met Gly Asn Ile Ala Ala Leu Phe Leu1460 1465 1470Arg Glu Asp Pro Cys Val Tyr Leu Cys Ser Ile Ala Thr Asp Ile1475 1480 1485Lys Ile Ala Ser Ser Met Ile Glu Gln Ile Gln Asp Lys Asn Ile1490 1495 1500Ser Phe Leu Leu Lys Asn Gly Ser Asp Ile Leu Val Glu Leu Asn1505 1510 1515Ala Glu Asp His Val Ala Ser Lys Pro Ser His Glu Ser Asp Pro1520 1525 1530
Met Val Tyr Asp Phe Asn Gln Val Lys Val Asp Ile Glu Gly Tyr1535 1540 1545Asp Ile Pro Leu Val Ser Glu Phe Ile Ile Lys Gln Pro Asp Gly1550 1555 1560Gly Tyr Asn Asp Ile Val Ile Glu Ser Pro Ile His Ile Lys Leu1565 1570 1575Lys Ser Lys Asp Thr Ser Asn Val Ile Ser Leu His Lys Met Pro1580 1585 1590Ser Gly Thr Gln Tyr Met Gln Ile Gly Pro Tyr Arg Thr Arg Leu1595 1600 1605Asn Thr Leu Phe Ser Arg Lys Leu Ala Glu Arg Ala Asn Ile Gly1610 1615 1620Ile Asp Asn Val Leu Ser Met Glu Thr Gln Asn Leu Pro Glu Pro1625 1630 1635Gln Leu Gly Glu Gly Phe Tyr Ala Thr Phe Lys Leu Pro Pro Tyr1640 1645 1650Asn Lys Glu Glu His Gly Asp Glu Arg Trp Phe Lys Ile His Ile1655 1660 1665Gly Asn Ile Asp Gly Asn Ser Ala Arg Gln Pro Tyr Tyr Glu Gly1670 1675 1680Met Leu Ser Asp Ile Glu Thr Thr Val Thr Leu Phe Val Pro Tyr1685 1690 1695
Ala Lys Gly Tyr Tyr Ile Arg Glu Gly Val Arg Leu Gly Val Gly1700 1705 1710Tyr Lys Lys Ile Ile Tyr Asp Lys Ser Trp Glu Ser Ala Phe Phe1715 1720 1725Tyr Phe Asp Glu Thr Lys Asn Gln Phe Ile Phe Ile Asn Asp Ala1730 1735 1740Asp His Asp Ser Gly Met Thr Gln Gln Gly Ile Val Lys Asn Ile1745 1750 1755Lys Lys Tyr Lys Gly Phe Ile His Val Val Val Met Lys Asn Asn1760 1765 1770Thr Glu Pro Met Asp Phe Asn Gly Ala Asn Ala Ile Tyr Phe Trp1775 1780 1785Glu Leu Phe Tyr Tyr Thr Pro Met Met Val Phe Gln Arg Leu Leu1790 1795 1800Gln Glu Gln Asn Phe Thr Glu Ser Thr Arg Trp Leu Arg Tyr Ile1805 1810 1815Trp Asn Pro Ala Gly Tyr Ser Val Gln Gly Glu Met Gln Asp Tyr1820 1825 1830Tyr Trp Asn Val Arg Pro Leu Glu Glu Asp Thr Ser Trp Asn Ala1835 1840 1845Asn Pro Leu Asp Ser Val Asp Pro Asp Ala Val Ala Gln His Asp1850 1855 1860
Pro Met His Tyr Lys Val Ala Thr Phe Met Lys Met Leu Asp Leu1865 1870 1875Leu Ile Thr Arg Gly Asp Ser Ala Tyr Arg Gln Leu Glu Arg Asp1880 1885 1890Thr Leu Asn Glu Ala Lys Met Trp Tyr Val Gln Ala Leu Thr Leu1895 1900 1905Leu Gly Asp Glu Pro Tyr Phe Ser Leu Asp Asn Asp Trp Ser Glu1910 1915 1920Pro Arg Leu Glu Glu Ala Ala Ser Gln Thr Met Arg His His Tyr1925 1930 1935Gln His Lys Met Leu Gln Leu Arg Gln Arg Ala Ala Leu Pro Thr1940 1945 1950Lys Arg Thr Ala Asn Ser Leu Thr Ala Leu Phe Leu Pro Gln Ile1955 1960 1965Asn Lys Lys Leu Gln Gly Tyr Trp Gln Thr Leu Thr Gln Arg Leu1970 1975 1980Tyr Asn Leu Arg His Asn Leu Thr Ile Asp Gly Gln Pro Leu Ser1985 1990 1995Leu Ser Leu Tyr Ala Thr Pro Ala Asp Pro Ser Met Leu Leu Ser2000 2005 2010Ala Ala Ile Thr Ala Ser Gln Gly Gly Gly Asp Leu Pro His Ala2015 2020 2025
Val Met Pro Met Tyr Arg Phe Pro Val Ile Leu Glu Asn Ala Lys2030 2035 2040Trp Gly Val Ser Gln Leu Ile Gln Phe Gly Asn Thr Leu Leu Ser2045 2050 2055Ile Thr Glu Arg Gln Asp Ala Glu Ala Leu Ala Glu Ile Leu Gln2060 2065 2070Thr Gln Gly Ser Glu Leu Ala Leu Gln Ser Ile Lys Met Gln Asp2075 2080 2085Lys Val Met Ala Glu Ile Asp Ala Asp Lys Leu Ala Leu Gln Glu2090 2095 2100Ser Arg His Gly Ala Gln Ser Arg Phe Asp Ser Phe Asn Thr Leu2105 2110 2115Tyr Asp Glu Asp Val Asn Ala Gly Glu Lys Gln Ala Met Asp Leu2120 2125 2130Tyr Leu Ser Ser Ser Val Leu Ser Thr Ser Gly Thr Ala Leu His2135 2140 2145Met Ala Ala Ala Ala Ala Asp Leu Val Pro Asn Ile Tyr Gly Phe2150 2155 2160Ala Val Gly Gly Ser Arg Phe Gly Ala Leu Phe Asn Ala Ser Ala2165 2170 2175Ile Gly Ile Glu Ile Ser Ala Ser Ala Thr Arg Ile Ala Ala Asp2180 2185 2190
Lys Ile Ser Gln Ser Glu Ile Tyr Arg Arg Arg Arg Gln Glu Trp2195 2200 2205Glu Ile Gln Arg Asn Asn Ala Glu Ala Glu Ile Lys Gln Ile Asp2210 2215 2220Ala Gln Leu Ala Thr Leu Ala Val Arg Arg Glu Ala Ala Val Leu2225 2230 2235Gln Lys Asn Tyr Leu Glu Thr Gln Gln Ala Gln Thr Gln Ala Gln2240 2245 2250Leu Ala Phe Leu Gln Ser Lys Phe Ser Asn Ala Ala Leu Tyr Asn2255 2260 2265Trp Leu Arg Gly Arg Leu Ser Ala Ile Tyr Tyr Gln Phe Tyr Asp2270 2275 2280Leu Ala Val Ser Leu Cys Leu Met Ala Glu Gln Thr Tyr Gln Tyr2285 2290 2295Glu Leu Asn Asn Ala Ala Ala His Phe Ile Lys Pro Gly Ala Trp2300 2305 2310His Gly Thr Tyr Ala Gly Leu Leu Ala Gly Glu Thr Leu Met Leu2315 2320 2325Asn Leu Ala Gln Met Glu Lys Ser Tyr Leu Glu Lys Asp Glu Arg2330 2335 2340Ala Leu Glu Val Thr Arg Thr Val Ser Leu Ala Glu Val Tyr Ala2345 2350 2355
Gly Leu Thr Glu Asn Ser Phe Ile Leu Lys Asp Lys Val Thr Glu2360 2365 2370Leu Val Asn Ala Gly Glu Gly Ser Ala Gly Thr Thr Leu Asn Gly2375 2380 2385Leu Asn Val Glu Gly Thr Gln Leu Gln Ala Ser Leu Lys Leu Ser2390 2395 2400Asp Leu Asn Ile Ala Thr Asp Tyr Pro Asp Gly Leu Gly Asn Thr2405 2410 2415Arg Arg Ile Lys Gln Ile Ser Val Thr Leu Pro Ala Leu Leu Gly2420 2425 2430Pro Tyr Gln Asp Val Arg Ala Ile Leu Ser Tyr Gly Gly Ser Thr2435 2440 2445Met Met Pro Arg Gly Cys Lys Ala Ile Ala Ile Ser His Gly Met2450 2455 2460Asn Asp Ser Gly Gln Phe Gln Met Asp Phe Asn Asp Ala Lys Tyr2465 2470 2475Leu Pro Phe Glu Gly Leu Pro Val Ala Asp Thr Gly Thr Leu Thr2480 2485 2490Leu Ser Phe Pro Gly Ile Ser Gly Lys Gln Lys Ser Leu Leu Leu2495 2500 2505Ser Leu Ser Asp Ile Ile Leu His Ile Arg Tyr Thr Ile Arg Ser2510 2515 2520
<210>15<211>3048<212>DNA<213>嗜线虫致病杆菌<400>15atgaagaatt tcgttcacag caatacgcca tccgtcaccg tactggacaa ccgtggtcag 60acagtacgcg aaatagcctg gtatcggcac cccgatacac ctcaggtaac cgatgaacgc120atcaccggtt atcaatatga tgctcaagga tctctgactc agagtattga tccgcgattt180tatgaacgcc agcagacagc gagtgacaag aacgccatta cacccaatct tattctcttg240tcatcactca gtaagaaggc attgcgtacg caaagtgtgg atgccggaac ccgtgtcgcc300ctgcatgatg ttgccgggcg tcccgtttta gctgtcagcg ccaatggcgt tagccgaacg360tttcagtatg aaagtgataa ccttccggga cgattgctaa cgattaccga gcaggtaaaa420ggagagaacg cctgtatcac ggagcgattg atctggtcag gaaatacgcc ggcagaaaaa480ggcaataatc tggccggcca gtgcgtggtc cattatgatc ccaccggaat gaatcaaacc540aacagcatat cgttaaccag catacccttg tccatcacac agcaattact gaaagatgac600agcgaagccg attggcacgg tatggatgaa tctggctgga aaaacgcgct ggcgccggaa660agcttcactt ctgtcagcac aacggatgct accggcacgg tattaacgag tacagatgct720gccggaaaca agcaacgtat cgcctatgat gtggccggtc tgcttcaagg cagttggttg780gcgctgaagg ggaaacaaga acaagttatc gtgaaatccc tgacctattc ggctgccagc840cagaagctac gggaggaaca tggtaacggg atagtgacta catataccta tgaacccgag900acgcaacgag ttattggcat aaaaacagaa cgtccttccg gtcatgccgc tggggagaaa960attttacaaa acctgcgtta tgaatatgat cctgtcggaa atgtgctgaa atcaactaat 1020gatgctgaaa ttacccgctt ttggcgcaac cagaaaattg taccggaaaa tacttacacc 1080tatgacagcc tgtaccagct ggtttccgtc actgggcgtg aaatggcgaa tattggccga 1140
caaaaaaacc agttacccat ccccgctctg attgataaca atacttatac gaattactct1200cgcacttacg actatgatcg tgggggaaat ctgaccagaa ttcgccataa ttcaccgatc1260accggtaata actatacaac gaacatgacc gtttcagatc acagcaaccg ggctgtactg1320gaagagctgg cgcaagatcc cactcaggtg gatatgttgt tcacccccgg cgggcatcag1380acccggcttg ttcccggtca ggatcttttc tggacacccc gtgacgaatt gcaacaagtg1440atattggtca atagggaaaa tacgacgcct gatcaggaat tctaccgtta tgatgcagac1500agtcagcgtg tcattaagac tcatattcag aagacaggta acagtgagca aatacagcga1560acattatatt tgccagagct ggaatggcgc acgacatata gcggcaatac attaaaagag1620tttttgcagg tcatcactgt cggtgaatcg ggtcaggcac aagtgcgggt gctgcattgg1680gaaacaggca aaccggcgga tatcagcaat gatcagctgc gctacagtta tggcaacctg1740attggcagta gcgggctgga attggacagt gacgggcaga tcattagtca ggaagaatat1800tacccctatg ggggaaccgc cgtgtgggca gcccgaagtc agtcagaagc tgattacaaa1860accgtgcgtt attctggcaa agagcgggat gcaacagggt tgtattacta cggttatcgt1920tattatcaat cgtggacagg gcgatggttg agtgtagatc ctgccggtga ggtcgatggt1980ctcaatttgt tccgaatgtg caggaataac cccatcgttt tttctgattc tgatggtcgt2040ttccccggtc agggtgtcct tgcctggata gggaaaaaag cgtatcgaaa ggcagtcaac2100atcacgacag aacacctgct tgaacaaggc gcttcctttg atacgttctt gaaattaaac2160cgaggattgc gaacgtttgt tttgggtgtg ggggtagcaa gtctgggggt gaaggcggcc2220acgattgcag gagcgtcgcc ttgggggatt gtcggggctg ccattggtgg ttttgtctcc2280ggggcggtga tggggttttt cgcgaacaac atctcagaaa aaattgggga agttttaagt2340tatctgacgc gtaaacgttc tgttcctgtt caggttggcg cttttgttgt cacatcgctt2400gtgacgtctg cactatttaa cagctcttcg acaggtaccg ccatttccgc agcaacagcg2460
gtcaccgttg gaggattaat ggctttagcc ggagagcata acacgggcat ggctatcagt 2520attgccacac ccgccggaca aggtacgctg gatacgctca ggcccggtaa tgtcagcgcg 2580ccagagcggt taggggcact atcaggcgca attattggcg gcatattact tggccgccat 2640cagggaagtt ctgagctggg tgaacgggca gcgattggtg ctatgtatgg tgctcgatgg 2700ggaaggatca ttggtaatct atgggatggc ccttatcggt ttatcggcag gttactgctc 2760agaagaggca ttagctctgc catttcccac gctgtcagtt ccaggagctg gtttggccga 2820atgataggag aaagtgtcgg gagaaatatt tctgaagtat tattacctta tagccgtaca 2880cccggtgaat gggttggtgc agccattggc gggacagccg cggccgctca tcatgccgtt 2940ggaggggaag ttgccaatgc cgctagccgg gttacctgga gcggctttaa gcgggctttt 3000aataacttct tctttaacgc ctctgcacgt cataatgaat ccgaagca 3048<210>16<211>1016<212>PRT<213>嗜线虫致病杆菌<400>16Met Lys Asn Phe Val His Ser Asn Thr Pro Ser Val Thr Val Leu Asp1 5 10 15Asn Arg Gly Gln Thr Val Arg Glu Ile Ala Trp Tyr Arg His Pro Asp20 25 30Thr Pro Gln Val Thr Asp Glu Arg Ile Thr Gly Tyr Gln Tyr Asp Ala35 40 45Gln Gly Ser Leu Thr Gln Ser Ile Asp Pro Arg Phe Tyr Glu Arg Gln50 55 60
Gln Thr Ala Ser Asp Lys Asn Ala Ile Thr Pro Asn Leu Ile Leu Leu65 70 75 80Ser Ser Leu Ser Lys Lys Ala Leu Arg Thr Gln Ser Val Asp Ala Gly85 90 95Thr Arg Val Ala Leu His Asp Val Ala Gly Arg Pro Val Leu Ala Val100 105 110Ser Ala Asn Gly Val Ser Arg Thr Phe Gln Tyr Glu Ser Asp Asn Leu115 120 125Pro Gly Arg Leu Leu Thr Ile Thr Glu Gln Val Lys Gly Glu Asn Ala130 135 140Cys Ile Thr Glu Arg Leu Ile Trp Ser Gly Asn Thr Pro Ala Glu Lys145 150 155 160Gly Asn Asn Leu Ala Gly Gln Cys Val Val His Tyr Asp Pro Thr Gly165 170 175Met Asn Gln Thr Asn Ser Ile Ser Leu Thr Ser Ile Pro Leu Ser Ile180 185 190Thr Gln Gln Leu Leu Lys Asp Asp Ser Glu Ala Asp Trp His Gly Met195 200 205Asp Glu Ser Gly Trp Lys Asn Ala Leu Ala Pro Glu Ser Phe Thr Ser210 215 220Val Ser Thr Thr Asp Ala Thr Gly Thr Val Leu Thr Ser Thr Asp Ala225 230 235 240
Ala Gly Asn Lys Gln Arg Ile Ala Tyr Asp Val Ala Gly Leu Leu Gln245 250 255Gly Ser Trp Leu Ala Leu Lys Gly Lys Gln Glu Gln Val Ile Val Lys260 265 270Ser Leu Thr Tyr Ser Ala Ala Ser Gln Lys Leu Arg Glu Glu His Gly275 280 285Asn Gly Ile Val Thr Thr Tyr Thr Tyr Glu Pro Glu Thr Gln Arg Val290 295 300Ile Gly Ile Lys Thr Glu Arg Pro Ser Gly His Ala Ala Gly Glu Lys305 310 315 320Ile Leu Gln Asn Leu Arg Tyr Glu Tyr Asp Pro Val Gly Asn Val Leu325 330 335Lys Ser Thr Asn Asp Ala Glu Ile Thr Arg Phe Trp Arg Asn Gln Lys340 345 350Ile Val Pro Glu Asn Thr Tyr Thr Tyr Asp Ser Leu Tyr Gln Leu Val355 360 365Ser Val Thr Gly Arg Glu Met Ala Asn Ile Gly Arg Gln Lys Asn Gln370 375 380Leu Pro Ile Pro Ala Leu Ile Asp Asn Asn Thr Tyr Thr Asn Tyr Ser385 390 395 400Arg Thr Tyr Asp Tyr Asp Arg Gly Gly Asn Leu Thr Arg Ile Arg His405 410 415
Asn Ser Pro Ile Thr Gly Asn Asn Tyr Thr Thr Asn Met Thr Val Ser420 425 430Asp His Ser Asn Arg Ala Val Leu Glu Glu Leu Ala Gln Asp Pro Thr435 440 445Gln Val Asp Met Leu Phe Thr Pro Gly Gly His Gln Thr Arg Leu Val450 455 460Pro Gly Gln Asp Leu Phe Trp Thr Pro Arg Asp Glu Leu Gln Gln Val465 470 475 480Ile Leu Val Asn Arg Glu Asn Thr Thr Pro Asp Gln Glu Phe Tyr Arg485 490 495Tyr Asp Ala Asp Ser Gln Arg Val Ile Lys Thr His Ile Gln Lys Thr500 505 510Gly Asn Ser Glu Gln Ile Gln Arg Thr Leu Tyr Leu Pro Glu Leu Glu515 520 525Trp Arg Thr Thr Tyr Ser Gly Asn Thr Leu Lys Glu Phe Leu Gln Val530 535 540Ile Thr Val Gly Glu Ser Gly Gln Ala Gln Val Arg Val Leu His Trp545 550 555 560Glu Thr Gly Lys Pro Ala Asp Ile Ser Asn Asp Gln Leu Arg Tyr Ser565 570 575Tyr Gly Asn Leu Ile Gly Ser Ser Gly Leu Glu Leu Asp Ser Asp Gly580 585 590
Gln Ile Ile Ser Gln Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr Ala Val595 600 605Trp Ala Ala Arg Ser Gln Ser Glu Ala Asp Tyr Lys Thr Val Arg Tyr610 615 620Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg625 630 635 640Tyr Tyr Gln Ser Trp Thr Gly Arg Trp Leu Ser Val Asp Pro Ala Gly645 650 655Glu Val Asp Gly Leu Asn Leu Phe Arg Met Cys Arg Asn Asn Pro Ile660 665 670Val Phe Ser Asp Ser Asp Gly Arg Phe Pro Gly Gln Gly Val Leu Ala675 680 685Trp Ile Gly Lys Lys Ala Tyr Arg Lys Ala Val Asn Ile Thr Thr Glu690 695 700His Leu Leu Glu Gln Gly Ala Ser Phe Asp Thr Phe Leu Lys Leu Asn705 710 715 720Arg Gly Leu Arg Thr Phe Val Leu Gly Val Gly Val Ala Ser Leu Gly725 730 735Val Lys Ala Ala Thr Ile Ala Gly Ala Ser Pro Trp Gly Ile Val Gly740 745 750Ala Ala Ile Gly Gly Phe Val Ser Gly Ala Val Met Gly Phe Phe Ala755 760 765
Asn Asn Ile Ser Glu Lys Ile Gly Glu Val Leu Ser Tyr Leu Thr Arg770 775 780Lys Arg Ser Val Pro Val Gln Val Gly Ala Phe Val Val Thr Ser Leu785 790 795 800Val Thr Ser Ala Leu Phe Asn Ser Ser Ser Thr Gly Thr Ala Ile Ser805 810 815Ala Ala Thr Ala Val Thr Val Gly Gly Leu Met Ala Leu Ala Gly Glu820 825 830His Asn Thr Gly Met Ala Ile Ser Ile Ala Thr Pro Ala Gly Gln Gly835 840 845Thr Leu Asp Thr Leu Arg Pro Gly Asn Val Ser Ala Pro Glu Arg Leu850 855 860Gly Ala Leu Ser Gly Ala Ile Ile Gly Gly Ile Leu Leu Gly Arg His865 870 875 880Gln Gly Ser Ser Glu Leu Gly Glu Arg Ala Ala Ile Gly Ala Met Tyr885 890 895Gly Ala Arg Trp Gly Arg Ile Ile Gly Asn Leu Trp Asp Gly Pro Tyr900 905 910Arg Phe Ile Gly Arg Leu Leu Leu Arg Arg Gly Ile Ser Ser Ala Ile915 920 925Ser His Ala Val Ser Ser Arg Ser Trp Phe Gly Arg Met Ile Gly Glu930 935 940
Ser Val Gly Arg Asn Ile Ser Glu Val Leu Leu Pro Tyr Ser Arg Thr945 950 955 960Pro Gly Glu Trp Val Gly Ala Ala Ile Gly Gly Thr Ala Ala Ala Ala965 970 975His His Ala Val Gly Gly Glu Val Ala Asn Ala Ala Ser Arg Val Thr980 985 990Trp Ser Gly Phe Lys Arg Ala Phe Asn Asn Phe Phe Phe Asn Ala Ser995 1000 1005Ala Arg His Asn Glu Ser Glu Ala1010 1015<210>17<211>4479<212>DNA<213>嗜线虫致病杆菌<400>17atgcagggtt caacaccttt gaaacttgaa ataccgtcat tgccctctgg gggcggatca 60ctaaaaggaa tgggagaagc actcaatgcc gtcggagcgg aagggggagc gtcattttca 120ctgcccttgc cgatctctgt cgggcgtggt ctggtgccgg tgctatcact gaattacagc 180agtactgccg gcaatgggtc attcgggatg gggtggcaat gtggggttgg ttttatcagc 240ctgcgtaccg ccaagggcgt tccgcactat acgggacaag atgagtatct cgggccggat 300ggggaagtgt tgagtattgt gccggacagc caagggcaac cagagcaacg caccgcaacc 360tcactgttgg ggacggttct gacacagccg catactgtta cccgctatca gtcccgcgtg 420gcagaaaaaa tcgttcgttt agaacactgg cagccacagc agagacgtga ggaagagacg 480tctttttggg tactttttac tgcggatggt ttagtgcacc tattcggtaa gcatcaccat 540
gcacgtattg ctgacccgca ggatgaaacc agaattgccc gctggctgat ggaggaaacc 600gtcacgcata ccggggaaca tatttactat cactatcggg cagaagacga tcttgactgt 660gatgagcatg aacttgctca gcattcaggt gttacggccc agcgttatct ggcaaaagtc 720agctatggca atactcagcc ggaaaccgct tttttcgcgg taaaatcagg tattcctgct 780gataatgact ggctgtttca tctggtattt gattacggtg agcgctcatc ttcgctgaac 840tctgtacccg aattcaatgt gtcagaaaac aatgtgtctg aaaacaatgt gcctgaaaaa 900tggcgttgtc gtccggacag tttctcccgc tatgaatatg ggtttgaaat tcgaacccgt 960cgcttgtgtc gccaagttct gatgtttcat cagctgaaag cgctggcagg ggaaaaggtt1020gcagaagaaa caccggcgct ggtttcccgt cttattctgg attatgacct gaacaacaag1080gtttccttgc tgcaaacggc ccgcagactg gcccatgaaa cggacggtac gccagtgatg1140atgtccccgc tggaaatgga ttatcaacgt gttaatcatg gcgtgaatct gaactggcag1200tccatgccgc agttagaaaa aatgaacacg ttgcagccat accaattggt tgatttatat1260ggagaaggaa tttccggcgt actttatcag gatactcaga aagcctggtg gtaccgtgct1320ccggtacggg atatcactgc cgaaggaacg aatgcggtta cctatgagga ggccaaacca1380ctgccacata ttccggcaca acaggaaagc gcgatgttgt tggacatcaa tggtgacggg1440cgtctggatt gggtgattac ggcatcaggg ttacggggct accacaccat gtcaccggaa1500ggtgaatgga caccctttat tccattatcc gctgtgccaa tggaatattt ccatccgcag1560gcaaaactgg ctgatattga tggggctggg ctgcctgact tagcgcttat cgggccaaat1620agtgtacgtg tctggtcaaa taatcgggca ggatgggatc gcgctcagga tgtgattcat1680ttgtcagata tgccactgcc ggttcccggc agaaatgagc gtcatcttgt cgcattcagt1740gatatgacag gctccgggca atcacatctg gtggaagtaa cggcagatag cgtgcgctac1800tggccgaacc tggggcatgg aaaatttggt gagcctctga tgatgacagg cttccagatt1860
agcggggaaa cgtttaaccc cgacagactg tatatggtag acatagatgg ctcaggcacc1920accgatttta tttatgcccg caatacttac cttgaactct atgccaatga aagcggcaat1980cattttgctg aacctcagcg tattgatctg ccggatgggg tacgttttga tgatacttgt2040cggttacaaa tagcggatac acaaggatta gggactgcca gcattatttt gacgatcccc2100catatgaagg tgcagcactg gcgattggat atgaccatat tcaagccttg gctgctgaat2160gccgtcaata acaatatggg aacagaaacc acgctgtatt atcgcagctc tgcccagttc2220tggctggatg agaaattaca ggcttctgaa tccgggatga cggtggtcag ctacttaccg2280ttcccggtgc atgtgttgtg gcgcacggaa gtgctggatg aaatttccgg taaccgattg2340accagccatt atcattactc acatggtgcc tgggatggtc tggaacggga gtttcgtggt2400tttgggcggg tgacacaaac tgatattgat tcacgggcga gtgcgacaca ggggacacat2460gctgaaccac cggcaccttc gcgcacggtt aattggtacg gcactggcgt acgggaagtc2520gatattcttc tgcccacgga atattggcag ggggatcaac aggcatttcc ccattttacc2580ccacgcttta cccgttatga cgaaaaatcc ggtggtgata tgacggtcac gccgagcgaa2640caggaagaat actggttaca tcgagcctta aaaggacaac gtttacgcag tgagctgtat2700ggggatgatg attctatact ggccggtacg ccttattcag tggatgaatc ccgcacccaa2760gtacgtttgt taccggtgat ggtatcggac gtgcctgcgg tactggtttc ggtggccgaa2820tcccgccaat accgatatga acgggttgct accgatccac agtgcagcca aaagatcgtc2880cttaaatctg atgcgttagg atttccgcag gacaatcttg agattgccta ttcgagacgt2940ccacagcctg agttctcgcc ttatccggat accctgcccg aaacactttt caccagcagt3000ttcgacgaac agcagatgtt ccttcgtctg acacgccagc gttcttctta tcatcatctg3060aatcatgatg ataatacgtg gatcacaggg cttatggata cctcacgcag tgacgcacgt3120atttatcaag ccgataaagt gccggacggt ggattttccc ttgaatggtt ttctgccaca3180
ggtgcaggag cattgttgtt gcctgatgcc gcagccgatt atctgggaca tcagcgtgta3240gcatataccg gtccagaaga acaacccgct attcctccgc tggtggcata cattgaaacc3300gcagagtttg atgaacgatc gttggcggct tttgaggagg tgatggatga gcaggagctg3360acaaaacagc tgaatgatgc gggctggaat acggcaaaag tgccgttcag tgaaaagaca3420gatttccatg tctgggtggg acaaaaggaa tttacagaat atgccggtgc agacggattc3480tatcggccat tggtgcaacg ggaaaccaag cttacaggta aaacgacagt cacgtgggat3540agccattact gtgttatcac cgcaacagag gatgcggctg gcctgcgtat gcaagcgcat3600tacgattatc gatttatggt tgcggataac accacagatg tcaatgataa ctatcacacc3660gtgacgtttg atgcactggg gagggtaacc agcttccgtt tctgggggac tgaaaacggt3720gaaaaacaag gatatacccc tgcggaaaat gaaactgtcc cctttattgt ccccacaacg3780gtggatgatg ctctggcatt gaaacccggt atacctgttg cagggctgat ggtttatgcc3840cctctgagct ggatggttca ggccagcttt tctaatgatg gggagcttta tggagagctg3900aaaccggctg ggatcatcac tgaagatggt tatctcctgt cgcttgcttt tcgccgctgg3960caacaaaata accctgccgc tgccatgcca aagcaagtca attcacagaa cccaccccat4020gtactgagtg tgatcaccga ccgctatgat gccgatccgg aacaacaatt acgtcaaacg4080tttacgttta gtgatggttt tgggcgaacc ttacaaacag ccgtacgcca tgaaagtggt4140gaagcctggg tacgtgatga gtatggagcc attgtggctg aaaatcatgg cgcgcctgaa4200acggcgatga cagatttccg ttgggcagtt tccggacgta cagaatatga cggaaaaggc4260caagccctgc gtaagtatca accgtatttc ctgaatagtt ggcagtacgt cagtgatgac4320agtgcccggc aggatatata tgccgatacc cattactatg atccgttggg gcgtgaatat4380caggttatca cggccaaagg cgggtttcgt cgatccttat tcactccctg gtttgtggtg4440aatgaagatg aaaatgacac tgccggtgaa atgacagca 4479
<210>18<211>1493<212>PRT<213>嗜线虫致病杆菌<400>18Met Gln Gly Ser Thr Pro Leu Lys Leu Glu Ile Pro Ser Leu Pro Ser1 5 10 15Gly Gly Gly Ser Leu Lys Gly Met Gly Glu Ala Leu Asn Ala Val Gly20 25 30Ala Glu Gly Gly Ala Ser Phe Ser Leu Pro Leu Pro Ile Ser Val Gly35 40 45Arg Gly Leu Val Pro Val Leu Ser Leu Asn Tyr Ser Ser Thr Ala Gly50 55 60Asn Gly Ser Phe Gly Met Gly Trp Gln Cys Gly Val Gly Phe Ile Ser65 70 75 80Leu Arg Thr Ala Lys Gly Val Pro His Tyr Thr Gly Gln Asp Glu Tyr85 90 95Leu Gly Pro Asp Gly Glu Val Leu Ser Ile Val Pro Asp Ser Gln Gly100105 110Gln Pro Glu Gln Arg Thr Ala Thr Ser Leu Leu Gly Thr Val Leu Thr115 120 125Gln Pro His Thr Val Thr Arg Tyr Gln Ser Arg Val Ala Glu Lys Ile130 135 140
Val Arg Leu Glu His Trp Gln Pro Gln Gln Arg Arg Glu Glu Glu Thr145 150 155 160Ser Phe Trp Val Leu Phe Thr Ala Asp Gly Leu Val His Leu Phe Gly165 170 175Lys His His His Ala Arg Ile Ala Asp Pro Gln Asp Glu Thr Arg Ile180 185 190Ala Arg Trp Leu Met Glu Glu Thr Val Thr His Thr Gly Glu His Ile195 200 205Tyr Tyr His Tyr Arg Ala Glu Asp Asp Leu Asp Cys Asp Glu His Glu210 215 220Leu Ala Gln His Ser Gly Val Thr Ala Gln Arg Tyr Leu Ala Lys Val225 230 235 240Ser Tyr Gly Asn Thr Gln Pro Glu Thr Ala Phe Phe Ala Val Lys Ser245 250 255Gly Ile Pro Ala Asp Asn Asp Trp Leu Phe His Leu Val Phe Asp Tyr260 265 270Gly Glu Arg Ser Ser Ser Leu Asn Ser Val Pro Glu Phe Asn Val Ser275 280 285Glu Asn Asn Val Ser Glu Asn Asn Val Pro Glu Lys Trp Arg Cys Arg290 295 300Pro Asp Ser Phe Ser Arg Tyr Glu Tyr Gly Phe Glu Ile Arg Thr Arg305 310 315 320
Arg Leu Cys Arg Gln Val Leu Met Phe His Gln Leu Lys Ala Leu Ala325 330 335Gly Glu Lys Val Ala Glu Glu Thr Pro Ala Leu Val Ser Arg Leu Ile340 345 350Leu Asp Tyr Asp Leu Asn Asn Lys Val Ser Leu Leu Gln Thr Ala Arg355 360 365Arg Leu Ala Hi s Glu Thr Asp Gly Thr Pro Val Met Met Ser Pro Leu370 375 380Glu Met Asp Tyr Gln Arg Val Asn His Gly Val Asn Leu Asn Trp Gln385 390 395 400Ser Met Pro Gln Leu Glu Lys Met Asn Thr Leu Gln Pro Tyr Gln Leu405 410 415Val Asp Leu Tyr Gly Glu Gly Ile Ser Gly Val Leu Tyr Gln Asp Thr420 425 430Gln Lys Ala Trp Trp Tyr Arg Ala Pro Val Arg Asp Ile Thr Ala Glu435 440 445Gly Thr Asn Ala Val Thr Tyr Glu Glu Ala Lys Pro Leu Pro His Ile450 455 460Pro Ala Gln Gln Glu Ser Ala Met Leu Leu Asp Ile Asn Gly Asp Gly465 470 475 480Arg Leu Asp Trp Val Ile Thr Ala Ser Gly Leu Arg Gly Tyr His Thr485 490 495
Met Ser Pro Glu Gly Glu Trp Thr Pro Phe Ile Pro Leu Ser Ala Val500 505 510Pro Met Glu Tyr Phe His Pro Gln Ala Lys Leu Ala Asp Ile Asp Gly515 520 525Ala Gly Leu Pro Asp Leu Ala Leu Ile Gly Pro Asn Ser Val Arg Val530 535 540Trp Ser Asn Asn Arg Ala Gly Trp Asp Arg Ala Gln Asp Val Ile His545 550 555 560Leu Ser Asp Met Pro Leu Pro Val Pro Gly Arg Asn Glu Arg His Leu565 570 575Val Ala Phe Ser Asp Met Thr Gly Ser Gly Gln Ser His Leu Val Glu580 585 590Val Thr Ala Asp Ser Val Arg Tyr Trp Pro Asn Leu Gly His Gly Lys595 600 605Phe Gly Glu Pro Leu Met Met Thr Gly Phe Gln Ile Ser Gly Glu Thr610 615 620Phe Asn Pro Asp Arg Leu Tyr Met Val Asp Ile Asp Gly Ser Gly Thr625 630 635 640Thr Asp Phe Ile Tyr Ala Arg Asn Thr Tyr Leu Glu Leu Tyr Ala Asn645 650 655Glu Ser Gly Asn His Phe Ala Glu Pro Gln Arg Ile Asp Leu Pro Asp660 665 670
Gly Val Arg Phe Asp Asp Thr Cys Arg Leu Gln Ile Ala Asp Thr Gln675 680 685Gly Leu Gly Thr Ala Ser Ile Ile Leu Thr Ile Pro His Met Lys Val690 695 700Gln His Trp Arg Leu Asp Met Thr Ile Phe Lys Pro Trp Leu Leu Asn705 710 715 720Ala Val Asn Asn Asn Met Gly Thr Glu Thr Thr Leu Tyr Tyr Arg Ser725 730 735Ser Ala Gln Phe Trp Leu Asp Glu Lys Leu Gln Ala Ser Glu Ser Gly740 745 750Met Thr Val Val Ser Tyr Leu Pro Phe Pro Val His Val Leu Trp Arg755 760 765Thr Glu Val Leu Asp Glu Ile Ser Gly Asn Arg Leu Thr Ser His Tyr770 775 780His Tyr Ser His Gly Ala Trp Asp Gly Leu Glu Arg Glu Phe Arg Gly785 790 795 800Phe Gly Arg Val Thr Gln Thr Asp Ile Asp Ser Arg Ala Ser Ala Thr805 810 815Gln Gly Thr His Ala Glu Pro Pro Ala Pro Ser Arg Thr Val Asn Trp820 825 830Tyr Gly Thr Gly Val Arg Glu Val Asp Ile Leu Leu Pro Thr Glu Tyr835 840 845
Trp Gln Gly Asp Gln Gln Ala Phe Pro His Phe Thr Pro Arg Phe Thr850 855 860Arg Tyr Asp Glu Lys Ser Gly Gly Asp Met Thr Val Thr Pro Ser Glu865 870 875 880Gln Glu Glu Tyr Trp Leu His Arg Ala Leu Lys Gly Gln Arg Leu Arg885 890 895Ser Glu Leu Tyr Gly Asp Asp Asp Ser Ile Leu Ala Gly Thr Pro Tyr900 905 910Ser Val Asp Glu Ser Arg Thr Gln Val Arg Leu Leu Pro Val Met Val915 920 925Ser Asp Val Pro Ala Val Leu Val Ser Val Ala Glu Ser Arg Gln Tyr930 935 940Arg Tyr Glu Arg Val Ala Thr Asp Pro Gln Cys Ser Gln Lys Ile Val945 950 955 960Leu Lys Ser Asp Ala Leu Gly Phe Pro Gln Asp Asn Leu Glu Ile Ala965 970 975Tyr Ser Arg Arg Pro Gln Pro Glu Phe Ser Pro Tyr Pro Asp Thr Leu980 985 990Pro Glu Thr Leu Phe Thr Ser Ser Phe Asp Glu Gln Gln Met Phe Leu995 1000 1005Arg Leu Thr Arg Gln Arg Ser Ser Tyr His His Leu Asn His Asp1010 1015 1020
Asp Asn Thr Trp Ile Thr Gly Leu Met Asp Thr Ser Arg Ser Asp1025 1030 1035Ala Arg Ile Tyr Gln Ala Asp Lys Val Pro Asp Gly Gly Phe Ser1040 1045 1050Leu Glu Trp Phe Ser Ala Thr Gly Ala Gly Ala Leu Leu Leu Pro1055 1060 1065Asp Ala Ala Ala Asp Tyr Leu Gly His Gln Arg Val Ala Tyr Thr1070 1075 1080Gly Pro Glu Glu Gln Pro Ala Ile Pro Pro Leu Val Ala Tyr Ile1085 1090 1095Glu Thr Ala Glu Phe Asp Glu Arg Ser Leu Ala Ala Phe Glu Glu1100 1105 1110Val Met Asp Glu Gln Glu Leu Thr Lys Gln Leu Asn Asp Ala Gly1115 1120 1125Trp Asn Thr Ala Lys Val Pro Phe Ser Glu Lys Thr Asp Phe His1130 1135 1140Val Trp Val Gly Gln Lys Glu Phe Thr Glu Tyr Ala Gly Ala Asp1145 1150 1155Gly Phe Tyr Arg Pro Leu Val Gln Arg Glu Thr Lys Leu Thr Gly1160 1165 1170Lys Thr Thr Val Thr Trp Asp Ser His Tyr Cys Val Ile Thr Ala1175 1180 1185
Thr Glu Asp Ala Ala Gly Leu Arg Met Gln Ala His Tyr Asp Tyr1190 1195 1200Arg Phe Met Val Ala Asp Asn Thr Thr Asp Val Asn Asp Asn Tyr1205 1210 1215His Thr Val Thr Phe Asp Ala Leu Gly Arg Val Thr Ser Phe Arg1220 1225 1230Phe Trp Gly Thr Glu Asn Gly Glu Lys Gln Gly Tyr Thr Pro Ala1235 1240 1245Glu Asn Glu Thr Val Pro Phe Ile Val Pro Thr Thr Val Asp Asp1250 1255 1260Ala Leu Ala Leu Lys Pro Gly Ile Pro Val Ala Gly Leu Met Val1265 1270 1275Tyr Ala Pro Leu Ser Trp Met Val Gln Ala Ser Phe Ser Asn Asp1280 1285 1290Gly Glu Leu Tyr Gly Glu Leu Lys Pro Ala Gly Ile Ile Thr Glu1295 1300 1305Asp Gly Tyr Leu Leu Ser Leu Ala Phe Arg Arg Trp Gln Gln Asn1310 1315 1320Asn Pro Ala Ala Ala Met Pro Lys Gln Val Asn Ser Gln Asn Pro1325 1330 1335Pro His Val Leu Ser Val Ile Thr Asp Arg Tyr Asp Ala Asp Pro1340 1345 1350
Glu Gln Gln Leu Arg Gln Thr Phe Thr Phe Ser Asp Gly Phe Gly1355 1360 1365Arg Thr Leu Gln Thr Ala Val Arg His Glu Ser Gly Glu Ala Trp1370 1375 1380Val Arg Asp Glu Tyr Gly Ala Ile Val Ala Glu Asn His Gly Ala1385 1390 1395Pro Glu Thr Ala Met Thr Asp Phe Arg Trp Ala Val Ser GlyArg1400 1405 1410Thr Glu Tyr Asp Gly Lys Gly Gln Ala Leu Arg Lys Tyr Gln Pro1415 1420 1425Tyr Phe Leu Asn Ser Trp Gln Tyr Val Ser Asp Asp Ser Ala Arg1430 1435 1440Gln Asp Ile Tyr Ala Asp Thr His Tyr Tyr Asp Pro Leu Gly Arg1445 1450 1455Glu Tyr Gln Val Ile Thr Ala Lys Gly Gly Phe Arg Arg Ser Leu1460 1465 1470Phe Thr Pro Trp Phe Val Val Asn Glu Asp Glu Asn Asp Thr Ala1475 1480 1485Gly Glu Met Thr Ala1490<210>19<211>7614<212>DNA<213>嗜线虫致病杆菌
<400>19atgtatagca cggctgtatt actcaataaa atcagtccca ctcgcgacgg tcagacgatg 60actcttgcgg atctgcaata tttatccttc agtgaactga gaaaaatctt tgatgaccag 120ctcagttggg gagaggctcg ccatctctat catgaaacta tagagcagaa aaaaaataat 180cgcttgctgg aagcgcgtat ttttacccgt gccaacccac aattatccgg tgctatccga 240ctcggtattg aacgagacag cgtttcacgc agttatgatg aaatgtttgg tgcccgttct 300tcttcctttg tgaaaccggg ttcagtggct tccatgtttt caccggctgg ctatctcacc 360gaattgtatc gtgaagcgaa ggacttacat ttttcaagct ctgcttatca tcttgataat 420cgccgtccgg atctggctga tctgactctg agccagagta atatggatac agaaatttcc 480accctgacac tgtctaacga actgttgctg gagcatatta cccgcaagac cggaggtgat 540tcggacgcat tgatggagag cctgtcaact taccgtcagg ccattgatac cccttaccat 600cagccttacg agactatccg tcaggtcatt atgacccatg acagtacact gtcagcgctg 660tcccgtaatc ctgaggtgat ggggcaggcg gaaggggctt cattactggc gattctggcc 720aatatttctc cggagcttta taacattttg accgaagaga ttacggaaaa gaacgctgat 780gctttatttg cgcaaaactt cagtgaaaat atcacgcccg aaaatttcgc gtcacaatca 840tggatagcca agtattatgg tcttgaactt tctgaggtgc aaaaatacct cgggatgttg 900cagaatggct attctgacag cacctctgct tatgtggata atatctcaac gggtttagtg 960gtcaataatg aaagtaaact cgaagcttac aaaataacac gtgtaaaaac agatgattat1020gataaaaata taaattactt tgatttgatg tatgaaggaa ataatcagtt ctttatacgt1080gctaatttta aggtatcaag agaatttggg gctactctta gaaaaaacgc agggccaagt1140ggcattgtcg gcagcctttc cggtcctcta atagccaata cgaattttaa aagtaattat1200ctaagtaaca tatctgattc tgaatacaaa aacggtgtaa agatatacgc ctatcgctat1260
acgtcttcca ccagcgccac aaatcagggc ggcggaatat tcacttttga gtcttatccc1320ctgactatat ttgcgctcaa actgaataaa gccattcgct tgtgcctgac tagcgggctt1380tcaccgaatg aactgcaaac tatcgtacgc agtgacaatg cacaaggcat catcaacgac1440tccgttctga ccaaagtttt ctatactctg ttctacagtc accgttatgc actgagcttt1500gatgatgcac aggtactgaa cggatcggtc attaatcaat atgccgacga tgacagtgtc1560agtcatttta accgtctctt taatacaccg ccgctgaaag ggaaaatctt tgaagccgac1620ggcaacacgg tcagcattga tccggatgaa gagcaatcta cctttgcccg ttcagccctg1680atgcgtggtc tgggggtcaa cagtggtgaa ctgtatcagt taggcaaact ggcgggtgtg1740ctggacgccc aaaataccat cacactttct gtcttcgtta tctcttcact gtatcgcctc1800acgttactgg cccgtgtcca tcagctgacg gtcaatgaac tgtgtatgct ttatggtctt1860tcgccgttca atggcaaaac aacggcttct ttgtcttccg gggagttgcc acggctggtt1920atctggctgt atcaggtgac gcagtggctg actgaggcgg aaatcaccac tgaagcgatc1980tggttattat gtacgccaga gtttagcggg aatatttcac cggaaatcag taatctgctc2040aataacctcc gaccgagtat tagtgaagat atggcacaga gtcacaatcg ggagctgcag2100gctgaaattc tcgcgccgtt tattgctgca acgctgcatc tggcgtcacc ggatatggca2160cggtatatcc tgttgtggac cgataacctg cggccgggtg gcttagatat tgccgggttt2220atgacactgg tattgaaaga gtcgttaaat gccaatgaaa ccacccaatt ggtacaattc2280tgccatgtga tggcacagtt atcgctttcc gtacagacac tgcgcctcag tgaagcggag2340ctatccgtgc tggtcatctc cggattcgcc gtgctggggg caaaaaatca acctgccgga2400cagcacaata ttgatacgct attctcactc taccgattcc accagtggat taatgggctg2460ggcaatcccg gctctgacac gctggatatg ctgcgccagc agacactcac ggccgacaga2520ctggcctccg tgatggggct ggacatcagt atggtaacgc aggccatggt ttccgccggc2580
gtgaaccagc ttcagtgttg gcaggatatc aacaccgtgt tgcagtggat agatgtggca2640tcagcactgc acacgatgcc gtcggttatc cgtacgctgg tgaatatccg ttacgtgact2700gcattaaaca aagccgagtc gaatctgcct tcctgggatg agtggcagac actggcagaa2760aatatggaag ccggactcag tacacaacag gctcagacgc tggcggatta taccgcggag2820cgcctgagta gcgtgctgtg caattggttt ctggcgaata tccagccaga aggggtgtcc2880ctgcacagcc gggatgacct gtacagctat ttcctgattg ataatcaggt ctcttctgcc2940ataaaaacca cccgactggc agaggccatt gccggtattc agctctacat caaccgggcg3000ctgaatcgga tagagcctaa tgcccgtgcc gatgtgtcaa cccgccagtt ttttaccgac3060tggacggtga ataaccgtta cagcacctgg ggcggggtgt cgcggctggt ttattatccg3120gaaaattaca ttgacccaac ccagcgtatc gggcagaccc ggatgatgga tgaactgctg3180gaaaatatca gccagagtaa acttagccgg gacacagtgg aggatgcctt taaaacttac3240ctgacccgct ttgaaaccgt ggcggatctg aaagttgtca gcgcctatca cgacaacgtc3300aacagcaaca ccggactgac ctggtttgtc ggccaaacgc gggagaacct gccggaatac3360tactggcgta acgtggatat atcacggatg caggcgggtg aactggccgc caatgcctgg3420aaagagtgga cgaagattga tacagcggtc aacccctaca aggatgcaat acgtccggtc3480atattcaggg aacgtttgca ccttatctgg gtagaaaaag aggaagtggc gaaaaatggt3540actgatccgg tggaaaccta tgaccgtttt actctgaaac tggcgtttct gcgtcatgat3600ggcagttgga gtgccccctg gtcttacgat atcacaacgc aggtggaggc ggtcactgac3660aaaaaacctg acactgaacg gctggcgctg gccgcatcag gctttcaggg cgaggacact3720ctgctggtgt ttgtctacaa aaccgggaag agttactcgg attttggcgg cagcaataaa3780aatgtggcag gcatgaccat ttacggcgat ggctccttca aaaagatgga gaacacagca3840ctcagccgtt acagccaact gaaaaatacc tttgatatca ttcatactca aggcaacgac3900
ttggtaagaa aggccagcta tcgtttcgcg caggattttg aagtgcctgc ctcgttgaat3960atgggttctg ccatcggtga tgatagtctg acggtgatgg agaacgggaa tattccgcag4020ataaccagta aatactccag cgataacctt gctattacgc tacataacgc cgctttcact4080gtcagatatg atggcagtgg caatgtcatc agaaacaaac aaatcagcgc catgaaactg4140acgggggtgg atggaaagtc ccagtacggc aatgcattta tcatcgcaaa taccgttaaa4200cattatggcg gttactctga tctggggggg ccgatcaccg tttataataa aacgaaaaac4260tatattgcat cagttcaagg ccacttgatg aacgcagatt acactaggcg tttgattcta4320acaccagttg aaaataatta ttatgccaga ttgttcgagt ttccattttc tccaaacaca4380attttaaaca ccgttttcac ggttggtagc aataaaacca gtgattttaa aaagtgcagt4440tatgctgttg atggtaataa ttctcagggc ttccagatat ttagttccta tcaatcatcc4500ggctggctgg atattgatac aggcattaac aataccgata tcaaaattac ggtgatggct4560ggcagtaaaa cccacacctt tacggccagt gaccatattg cttccttgcc ggcaaacagt4620tttgatgcta tgccgtacac ctttaagcca ctggaaatcg atgcttcatc gttggccttt4680accaataata ttgctcctct ggatatcgtt tttgagacca aagccaaaga cgggcgagtg4740ctgggtaaga tcaagcaaac attatcggtg aaacgggtaa attataatcc ggaagatatt4800ctgtttctgc gtgaaactca ttcgggtgcc caatatatgc agctcggggt gtatcgtatt4860cgtcttaata ccctgctggc ttctcaactg gtatccagag caaacacggg cattgatact4920atcctgacaa tggaaaccca gcggttaccg gaacctccgt tgggagaagg cttctttgcc4980aactttgttc tgcctaaata tgaccctgct gaacatggcg atgagcggtg gtttaaaatc5040catattggga atgttggcgg taacacggga aggcagcctt attacagcgg aatgttatcc5100gatacgtcgg aaaccagtat gacactgttt gtcccttatg ccgaagggta ttacatgcat5160gaaggtgtca gattgggggt tggataccag aaaattacct atgacaacac ttgggaatct5220
gctttctttt attttgatga gacaaaacag caatttgtat taattaacga tgctgatcat5280gattcaggaa tgacgcaaca ggggatcgtg aaaaatatca agaaatacaa aggatttttg5340aatgtttcta tcgcaacggg ctattccgcc ccgatggatt tcaatagtgc cagcgccctc5400tattactggg aattgttcta ttacaccccg atgatgtgct tccagcgttt gctacaggaa5460aaacaattcg acgaagccac acaatggata aactacgtct acaatcccgc cggctatatc5520gttaacggag aaatcgcccc ctggatctgg aactgccggc cgctggaaga gaccacctcc5580tggaatgcca atccgctgga tgccatcgat ccggatgccg tcgcccaaaa tgacccaatg5640cactacaaga ttgccacctt tatgcgcctg ttggatcaac ttattctgcg cggcgatatg5700gcctatcgag aactgacccg cgatgcgttg aatgaagcca aaatgtggta tgtgcgtact5760ttagaattgc tcggtgatga gccggaggat tacggtagcc aacagtgggc agcaccgtcc5820ctttccgggg cggcgagtca aaccgtgcag gcggcttatc agcaggatct tacgatgctg5880ggccgtggtg gggtttccaa gaatctccgt accgctaact cgttggtggg tttgttcctg5940ccggaatata acccggcgct caccgattac tggcaaaccc tgcgtttgcg cctgtttaac6000ctgcgccata atctttccat tgacggacag ccgttatcgc tggcgattta cgccgagcct6060accgatccga aagcgctgct caccagtatg gtacaggcct ctcagggcgg tagtgcagtg6120ctgcccggca cattgtcgtt ataccgcttc ccggtgatgc tggagcggac ccgcaatctg6180gtagcgcaat taacccagtt cggcacctct ctgctcagta tggcagagca tgatgatgcc6240gatgaactca ccacgctgct actacagcag ggtatggaac tggcgacaca gagcatccgt6300attcagcaac gaactgtcga tgaagtggat gctgatattg ctgtattggc agagagccgc6360cgcagtgcac aaaatcgtct ggaaaaatac cagcagctgt atgacgagga tatcaaccac6420ggagaacagc gggcaatgtc actgcttgat gcagcggcag gtcagtctct ggccgggcag6480gtgctttcaa tagcggaagg ggtggccgat ttagtgccaa acgtgttcgg tttagcttgt6540
ggcggcagtc gttggggggc agcactgcgt gcttccgcct ccgtgatgtc gctttctgcc 6600acagcttccc aatattccgc agacaaaatc agccgttcgg aagcctaccg ccgccgccgt 6660caggagtggg aaattcagcg tgataatgct gacggtgaag tcaaacaaat ggatgcccag 6720ttggaaagcc tgaaaatccg ccgcgaagca gcacagatgc aggtggaata tcaggagacc 6780cagcaggccc atactcaggc tcagttagag ctgttacagc gtaaattcac aaacaaagcg 6840ctttacagtt ggatgcgcgg caagctgagt gctatctatt accagttctt tgacctgacc 6900cagtccttct gcctgatggc acaggaagcg ctgcgccgcg agctgaccga caacggtgtt 6960acctttatcc ggggtggggc ctggaacggt acgactgcgg gtttgatggc gggtgaaacg 7020ttgctgctga atctggcaga aatggaaaaa gtctggctgg agcgtgatga gcgggcactg 7080gaagtgaccc gtaccgtctc gttggcacag ttctatcagg ccttatcatc agacaacttt 7140aatctgaccg aaaaactcac gcaattcctg cgtgaaggga aaggcaacgt aggagcttcc 7200ggcaatgaat taaaactcag taaccgtcag atagaagcct cagtgcgatt gtctgatttg 7260aaaattttca gcgactaccc cgaaagcctt ggcaataccc gtcagttgaa acaggtgagt 7320gtcaccttgc cggcgctggt tgggccgtat gaagatattc gggcggtgct gaattacggg 7380ggcagcatcg tcatgccacg cggttgcagt gctattgctc tctcccacgg cgtgaatgac 7440agtggtcaat ttatgctgga tttcaacgat tcccgttatc tgccgtttga aggtatttcc 7500gtgaatgaca gcggcagcct gacgttgagt ttcccggatg cgactgatcg gcagaaagcg 7560ctgctggaga gcctgagcga tatcattctg catatccgct ataccattcg ttct7614<210>20<211>2538<212>PRT<213>嗜线虫致病杆菌<400>20
Met Tyr Ser Thr Ala Val Leu Leu Asn Lys Ile Ser Pro Thr Arg Asp1 5 10 15Gly Gln Thr Met Thr Leu Ala Asp Leu Gln Tyr Leu Ser Phe Ser Glu20 25 30Leu Arg Lys Ile Phe Asp Asp Gln Leu Ser Trp Gly Glu Ala Arg His35 40 45Leu Tyr His Glu ThrIle Glu Gln Lys Lys Asn Asn Arg Leu Leu Glu50 55 60Ala Arg Ile Phe Thr Arg Ala Asn Pro Gln Leu Ser Gly Ala Ile Arg65 70 75 80Leu Gly Ile Glu Arg Asp Ser Val Ser Arg Ser Tyr Asp Glu Met Phe85 90 95Gly Ala Arg Ser Ser Ser Phe Val Lys Pro Gly Ser Val Ala Ser Met100 105 110Phe Ser Pro Ala Gly Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asp115 120 125Leu His Phe Ser Ser Ser Ala Tyr His Leu Asp Asn Arg Arg Pro Asp130 135 140Leu Ala Asp Leu Thr Leu Ser Gln Ser Asn Met Asp Thr Glu Ile Ser145 150 155 160Thr Leu Thr Leu Ser Asn Glu Leu Leu Leu Glu His Ile Thr Arg Lys165 170 175
Thr Gly Gly Asp Ser Asp Ala Leu Met Glu Ser Leu Ser Thr Tyr Arg180 185 190Gln Ala Ile Asp Thr Pro Tyr His Gln Pro Tyr Glu Thr Ile Arg Gln195 200 205Val Ile Met Thr His Asp Ser Thr Leu Ser Ala Leu Ser Arg Asn Pro210 215 220Glu Val Met Gly Gln Ala Glu Gly Ala Ser Leu Leu Ala Ile Leu Ala225 230 235 240Asn Ile Ser Pro Glu Leu Tyr Asn Ile Leu Thr Glu Glu Ile Thr Glu245 250 255Lys Asn Ala Asp Ala Leu Phe Ala Gln Asn Phe Ser Glu Asn Ile Thr260 265 270Pro Glu Asn Phe Ala Ser Gln Ser Trp Ile Ala Lys Tyr Tyr Gly Leu275 280 285Glu Leu Ser Glu Val Gln Lys Tyr Leu Gly Met Leu Gln Asn Gly Tyr290 295 300Ser Asp Ser Thr Ser Ala Tyr Val Asp Asn Ile Ser Thr Gly Leu Val305 310 315 320Val Asn Asn Glu Ser Lys Leu Glu Ala Tyr Lys Ile Thr Arg Val Lys325 330 335Thr Asp Asp Tyr Asp Lys Asn Ile Asn Tyr Phe Asp Leu Met Tyr Glu340 345 350
Gly Asn Asn Gln Phe Phe Ile Arg Ala Asn Phe Lys Val Ser Arg Glu355 360 365Phe Gly Ala Thr Leu Arg Lys Asn Ala Gly Pro Ser Gly Ile Val Gly370 375 380Ser Leu Ser Gly Pro Leu Ile Ala Asn Thr Asn Phe Lys Ser Asn Tyr385 390 395 400Leu Ser Asn Ile Ser Asp Ser Glu Tyr Lys Asn Gly Val Lys Ile Tyr405 410 415Ala Tyr Arg Tyr Thr Ser Ser Thr Ser Ala Thr Asn Gln Gly Gly Gly420 425 430Ile Phe Thr Phe Glu Ser Tyr Pro Leu Thr Ile Phe Ala Leu Lys Leu435 440 445Asn Lys Ala Ile Arg Leu Cys Leu Thr Ser Gly Leu Ser Pro Asn Glu450 455 460Leu Gln Thr Ile Val Arg Ser Asp Asn Ala Gln Gly Ile Ile Asn Asp465 470 475 480Ser Val Leu Thr Lys Val Phe Tyr Thr Leu Phe Tyr Ser His Arg Tyr485 490 495Ala Leu Ser Phe Asp Asp Ala Gln Val Leu Asn Gly Ser Val Ile Asn500 505 510Gln Tyr Ala Asp Asp Asp Ser Val Ser His Phe Asn Arg Leu Phe Asn515 520 525
Thr Pro Pro Leu Lys Gly Lys Ile Phe Glu Ala Asp Gly Asn Thr Val530 535 540Ser Ile Asp Pro Asp Glu Glu Gln Ser Thr Phe Ala Arg Ser Ala Leu545 550 555 560Met Arg Gly Leu Gly Val Asn Ser Gly Glu Leu Tyr Gln Leu Gly Lys565 570 575Leu Ala Gly Val Leu Asp Ala Gln Asn Thr Ile Thr Leu Ser Val Phe580 585 590Val Ile Ser Ser Leu Tyr Arg Leu Thr Leu Leu Ala Arg Val His Gln595 600 605Leu Thr Val Asn Glu Leu Cys Met Leu Tyr Gly Leu Ser Pro Phe Asn610 615 620Gly Lys Thr Thr Ala Ser Leu Ser Ser Gly Glu Leu Pro Arg Leu Val625 630 635 640Ile Trp Leu Tyr Gln Val Thr Gln Trp Leu Thr Glu Ala Glu Ile Thr645 650 655Thr Glu Ala Ile Trp Leu Leu Cys Thr Pro Glu Phe Ser Gly Asn Ile660 665 670Ser Pro Glu Ile Ser Asn Leu Leu Asn Asn Leu Arg Pro Ser Ile Ser675 680 685Glu Asp Met Ala Gln Ser His Asn Arg Glu Leu Gln Ala Glu Ile Leu690 695 700
Ala Pro Phe Ile Ala Ala Thr Leu His Leu Ala Ser Pro Asp Met Ala705 710 715 720Arg Tyr Ile Leu Leu Trp Thr Asp Asn Leu Arg Pro Gly Gly Leu Asp725 730 735Ile Ala Gly Phe Met Thr Leu Val Leu Lys Glu Ser Leu Asn Ala Asn740 745 750Glu Thr Thr Gln Leu Val Gln Phe Cys His Val Met Ala Gln Leu Ser755 760 765Leu Ser Val Gln Thr Leu Arg Leu Ser Glu Ala Glu Leu Ser Val Leu770 775 780Val Ile Ser Gly Phe Ala Val Leu Gly Ala Lys Asn Gln Pro Ala Gly785 790 795 800Gln His Asn Ile Asp Thr Leu Phe Ser Leu Tyr Arg Phe His Gln Trp805 810 815Ile Asn Gly Leu Gly Asn Pro Gly Ser Asp Thr Leu Asp Met Leu Arg820 825 830Gln Gln Thr Leu Thr Ala Asp Arg Leu Ala Ser Val Met Gly Leu Asp835 840 845Ile Ser Met Val Thr Gln Ala Met Val Ser Ala Gly Val Asn Gln Leu850 855 860Gln Cys Trp Gln Asp Ile Asn Thr Val Leu Gln Trp Ile Asp Val Ala865 870 875 880
Ser Ala Leu His Thr Met Pro Ser Val Ile Arg Thr Leu Val Asn Ile885 890 895Arg Tyr Val Thr Ala Leu Asn Lys Ala Glu Ser Asn Leu Pro Ser Trp900 905 910Asp Glu Trp Gln Thr Leu Ala Glu Asn Met Glu Ala Gly Leu Ser Thr915 920 925Gln Gln Ala Gln Thr Leu Ala Asp Tyr Thr Ala Glu Arg Leu Ser Ser930 935 940Val Leu Cys Asn Trp Phe Leu Ala Asn Ile Gln Pro Glu Gly Val Ser945 950 955 960Leu His Ser Arg Asp Asp Leu Tyr Ser Tyr Phe Leu Ile Asp Asn Gln965 970 975Val Ser Ser Ala Ile Lys Thr Thr Arg Leu Ala Glu Ala Ile Ala Gly980 985 990Ile Gln Leu Tyr Ile Asn Arg Ala Leu Asn Arg Ile Glu Pro Asn Ala995 1000 1005Arg Ala Asp Val Ser Thr Arg Gln Phe Phe Thr Asp Trp Thr Val1010 1015 1020Asn Asn Arg Tyr Ser Thr Trp Gly Gly Val Ser Arg Leu Val Tyr1025 1030 1035Tyr Pro Glu Asn Tyr Ile Asp Pro Thr Gln Arg Ile Gly Gln Thr1040 1045 1050
Arg Met Met Asp Glu Leu Leu Glu Asn Ile Ser Gln Ser Lys Leu1055 1060 1065Ser Arg Asp Thr Val Glu Asp Ala Phe Lys Thr Tyr Leu Thr Arg1070 1075 1080Phe Glu Thr Val Ala Asp Leu Lys Val Val Ser Ala Tyr His Asp1085 1090 1095Asn Val Asn Ser Asn Thr Gly Leu Thr Trp Phe Val Gly Gln Thr1100 1105 1110Arg Glu Asn Leu Pro Glu Tyr Tyr Trp Arg Asn Val Asp Ile Ser1115 1120 1125Arg Met Gln Ala Gly Glu Leu Ala Ala Asn Ala Trp Lys Glu Trp1130 1135 1140Thr Lys Ile Asp Thr Ala Val Asn Pro Tyr Lys Asp Ala Ile Arg1145 1150 1155Pro Val Ile Phe Arg Glu Arg Leu His Leu Ile Trp Val Glu Lys1160 1165 1170Glu Glu Val Ala Lys Asn Gly Thr Asp Pro Val Glu Thr Tyr Asp1175 1180 1185Arg Phe Thr Leu Lys Leu Ala Phe Leu Arg His Asp Gly Ser Trp1190 1195 1200Ser Ala Pro Trp Ser Tyr Asp Ile Thr Thr Gln Val Glu Ala Val1205 1210 1215
Thr Asp Lys Lys Pro Asp Thr Glu Arg Leu Ala Leu Ala Ala Ser1220 1225 1230Gly Phe Gln Gly Glu Asp Thr Leu Leu Val Phe Val Tyr Lys Thr1235 1240 1245Gly Lys Ser Tyr Ser Asp Phe Gly Gly Ser Asn Lys Asn Val Ala1250 1255 1260Gly Met Thr Ile Tyr Gly Asp Gly Ser Phe Lys Lys Met Glu Asn1265 1270 1275Thr Ala Leu Ser Arg Tyr Ser Gln Leu Lys Asn Thr Phe Asp Ile1280 1285 1290Ile His Thr Gln Gly Asn Asp Leu Val Arg Lys Ala Ser Tyr Arg1295 1300 1305Phe Ala Gln Asp Phe Glu Val Pro Ala Ser Leu Asn Met Gly Ser1310 1315 1320Ala Ile Gly Asp Asp Ser Leu Thr Val Met Glu Asn Gly Asn Ile1325 1330 1335Pro Gln Ile Thr Ser Lys Tyr Ser Ser Asp Asn Leu Ala Ile Thr1340 1345 1350Leu His Asn Ala Ala Phe Thr Val Arg Tyr Asp Gly Ser Gly Asn1355 1360 1365Val Ile Arg Asn Lys Gln Ile Ser Ala Met Lys Leu Thr Gly Val1370 1375 1380
Asp Gly Lys Ser Gln Tyr Gly Asn Ala Phe Ile Ile Ala Asn Thr1385 1390 1395Val Lys His Tyr Gly Gly Tyr Ser Asp Leu Gly Gly Pro Ile Thr1400 1405 1410Val Tyr Asn Lys Thr Lys Asn Tyr Ile Ala Ser Val Gln Gly His1415 1420 1425Leu Met Asn Ala Asp Tyr Thr Arg Arg LeuIle Leu Thr Pro Val1130 1435 1440Glu Asn Asn Tyr Tyr Ala Arg Leu Phe Glu Phe Pro Phe Ser Pro1445 1450 1455Asn Thr Ile Leu Asn Thr Val Phe Thr Val Gly Ser Asn Lys Thr1460 1465 1470Ser Asp Phe Lys Lys Cys Ser Tyr Ala Val Asp Gly Asn Asn Ser1475 1480 1485Gln Gly Phe Gln Ile Phe Ser Ser Tyr Gln Ser Ser Gly Trp Leu1490 1495 1500Asp Ile Asp Thr Gly Ile Asn Asn Thr Asp Ile Lys Ile Thr Val1505 1510 1515Met Ala Gly Ser Lys Thr His Thr Phe Thr Ala Ser Asp His Ile1520 1525 1530Ala Ser Leu Pro Ala Asn Ser Phe Asp Ala Met Pro Tyr Thr Phe1535 1540 1545
Lys Pro Leu Glu Ile Asp Ala Ser Ser Leu Ala Phe Thr Asn Asn1550 1555 1560Ile Ala Pro Leu Asp Ile Val Phe Glu Thr Lys Ala Lys Asp Gly1565 1570 1575Arg Val Leu Gly Lys Ile Lys Gln Thr Leu Ser Val Lys Arg Val1580 1585 1590Asn Tyr Asn Pro Glu Asp Ile Leu Phe Leu Arg Glu Thr His Ser1595 1600 1605Gly Ala Gln Tyr Met Gln Leu Gly Val Tyr Arg Ile Arg Leu Asn1610 1615 1620Thr Leu Leu Ala Ser Gln Leu Val Ser Arg Ala Asn Thr Gly Ile1625 1630 1635Asp Thr Ile Leu Thr Met Glu Thr Gln Arg Leu Pro Glu Pro Pro1640 1645 1650Leu Gly Glu Gly Phe Phe Ala Asn Phe Val Leu Pro Lys Tyr Asp1655 1660 1665Pro Ala Glu His Gly Asp Glu Arg Trp Phe Lys Ile His Ile Gly1670 1675 1680Asn Val Gly Gly Asn Thr Gly Arg Gln Pro Tyr Tyr Ser Gly Met1685 1690 1695Leu Ser Asp Thr Ser Glu Thr Ser Met Thr Leu Phe Val Pro Tyr1700 1705 1710
Ala Glu Gly Tyr Tyr Met His Glu Gly Val Arg Leu Gly Val Gly1715 1720 1725Tyr Gln Lys Ile Thr Tyr Asp Asn Thr Trp Glu Ser Ala Phe Phe1730 1735 1740Tyr Phe Asp Glu Thr Lys Gln Gln Phe Val Leu Ile Asn Asp Ala1745 1750 1755Asp His Asp Ser Gly Met Thr Gln Gln Gly Ile Val Lys Asn Ile1760 1765 1770Lys Lys Tyr Lys Gly Phe Leu Asn Val Ser Ile Ala Thr Gly Tyr1775 1780 1785Ser Ala Pro Met Asp Phe Asn Ser Ala Ser Ala Leu Tyr Tyr Trp1790 1795 1800Glu Leu Phe Tyr Tyr Thr Pro Met Met Cys Phe Gln Arg Leu Leu1805 1810 1815Gln Glu Lys Gln Phe Asp Glu Ala Thr Gln Trp Ile Asn Tyr Val1820 1825 1830Tyr Asn Pro Ala Gly Tyr Ile Val Asn Gly Glu Ile Ala Pro Trp1835 1840 1845Ile Trp Asn Cys Arg Pro Leu Glu Glu Thr Thr Ser Trp Asn Ala1850 1855 1860Asn Pro Leu Asp Ala Ile Asp Pro Asp Ala Val Ala Gln Asn Asp1865 1870 1875
Pro Met His Tyr Lys Ile Ala Thr Phe Met Arg Leu Leu Asp Gln1880 1885 1890Leu Ile Leu Arg Gly Asp Met Ala Tyr Arg Glu Leu Thr Arg Asp1895 1900 1905Ala Leu Asn Glu Ala Lys Met Trp Tyr Val Arg Thr Leu Glu Leu1910 1915 1920Leu Gly Asp Glu Pro Glu Asp Tyr Gly Ser Gln Gln Trp Ala Ala1925 1930 1935Pro Ser Leu Ser Gly Ala Ala Ser Gln Thr Val Gln Ala Ala Tyr1940 1945 1950Gln Gln Asp Leu Thr Met Leu Gly Arg Gly Gly Val Ser Lys Asn1955 1960 1965Leu Arg Thr Ala Asn Ser Leu Val Gly Leu Phe Leu Pro Glu Tyr1970 1975 1980Asn Pro Ala Leu Thr Asp Tyr Trp Gln Thr Leu Arg Leu Arg Leu1985 1990 1995Phe Asn Leu Arg His Asn Leu Ser Ile Asp Gly Gln Pro Leu Ser2000 2005 2010Leu Ala Ile Tyr Ala Glu Pro Thr Asp Pro Lys Ala Leu Leu Thr2015 2020 2025Ser Met Val Gln Ala Ser Gln Gly Gly Ser Ala Val Leu Pro Gly2030 2035 2040
Thr Leu Ser Leu Tyr Arg Phe Pro Val Met Leu Glu Arg Thr Arg2045 2050 2055Asn Leu Val Ala Gln Leu Thr Gln Phe Gly Thr Ser Leu Leu Ser2060 2065 2070Met Ala Glu His Asp Asp Ala Asp Glu Leu Thr Thr Leu Leu Leu2075 2080 2085Gln Gln Gly Met Glu Leu Ala Thr Gln Ser Ile Arg Ile Gln Gln2090 2095 2100Arg Thr Val Asp Glu Val Asp Ala Asp Ile Ala Val Leu Ala Glu2105 2110 2115Ser Arg Arg Ser Ala Gln Asn Arg Leu Glu Lys Tyr Gln Gln Leu2120 2125 2130Tyr Asp Glu Asp Ile Asn His Gly Glu Gln Arg Ala Met Ser Leu2135 2140 2145Leu Asp Ala Ala Ala Gly Gln Ser Leu Ala Gly Gln Val Leu Ser2150 2155 2160Ile Ala Glu Gly Val Ala Asp Leu Val Pro Asn Val Phe Gly Leu2165 2170 2175Ala Cys Gly Gly Ser Arg Trp Gly Ala Ala Leu Arg Ala Ser Ala2180 2185 2190Ser Val Met Ser Leu Ser Ala Thr Ala Ser Gln Tyr Ser Ala Asp2195 2200 2205
Lys Ile Ser Arg Ser Glu Ala Tyr Arg Arg Arg Arg Gln Glu Trp2210 2215 2220Glu Ile Gln Arg Asp Asn Ala Asp Gly Glu Val Lys Gln Met Asp2225 2230 2235Ala Gln Leu Glu Ser Leu Lys Ile Arg Arg Glu Ala Ala Gln Met2240 2245 2250Gln Val Glu Tyr Gln Glu Thr Gln Gln Ala His Thr Gln Ala Gln2255 2260 2265Leu Glu Leu Leu Gln Arg Lys Phe Thr Asn Lys Ala Leu Tyr Ser2270 2275 2280Trp Met Arg Gly Lys Leu Ser Ala Ile Tyr Tyr Gln Phe Phe Asp2285 2290 2295Leu Thr Gln Ser Phe Cys Leu Met Ala Gln Glu Ala Leu Arg Arg2300 2305 2310Glu Leu Thr Asp Asn Gly Val Thr Phe Ile Arg Gly Gly Ala Trp2315 2320 2325Asn Gly Thr Thr Ala Gly Leu Met Ala Gly Glu Thr Leu Leu Leu2330 2335 2340Asn Leu Ala Glu Met Glu Lys Val Trp Leu Glu Arg Asp Glu Arg2345 2350 2355Ala Leu Glu Val Thr Arg Thr Val Ser Leu Ala Gln Phe Tyr Gln2360 2365 2370
Ala Leu Ser Ser Asp Asn Phe Asn Leu Thr Glu Lys Leu Thr Gln2375 2380 2385Phe Leu Arg Glu Gly Lys Gly Asn Val Gly Ala Ser Gly Asn Glu2390 2395 2400Leu Lys Leu Ser Asn Arg Gln Ile Glu Ala Ser Val Arg Leu Ser2405 2410 2415Asp Leu Lys Ile Phe Ser Asp Tyr Pro Glu Ser Leu Gly Asn Thr2420 2425 2430Arg Gln Leu Lys Gln Val Ser Val Thr Leu Pro Ala Leu Val Gly2435 2440 2445Pro Tyr Glu Asp Ile Arg Ala Val Leu Asn Tyr Gly Gly Ser Ile2450 2455 2460Val Met Pro Arg Gly Cys Ser Ala Ile Ala Leu Ser His Gly Val2465 2470 2475Asn Asp Ser Gly Gln Phe Met Leu Asp Phe Asn Asp Ser Arg Tyr2480 2485 2490Leu Pro Phe Glu Gly Ile Ser Val Asn Asp Ser Gly Ser Leu Thr2495 2500 2505Leu Ser Phe Pro Asp Ala Thr Asp Arg Gln Lys Ala Leu Leu Glu2510 2515 2520Ser Leu Ser Asp Ile Ile Leu His Ile Arg Tyr Thr Ile Arg Ser2525 2530 2535
<210>21<211>7551<212>DNA<213>发光光杆状菌(Photorhabdus luminescens)<220>
<221>外显子<222>(1)..(7551)<400>21atg aac gag tct gta aaa gag ata cct gat gta tta aaa agc cag tgt 48Met Asn Glu Ser Val Lys Glu Ile Pro Asp Val Leu Lys Ser Gln Cys1 5 10 15ggt ttt aat tgt ctg aca gat att agc cac agc tct ttt aat gaa ttt 96Gly Phe Asn Cys Leu Thr Asp Ile Ser His Ser Ser Phe Asn Glu Phe20 25 30cgc cag caa gta tct gag cac ctc tcc tgg tcc gaa aca cac gac tta144Arg Gln Gln Val Ser Glu His Leu Ser Trp Ser Glu Thr His Asp Leu35 40 45tat cat gat gca caa cag gca caa aag gat aat cgc ctg tat gaa gcg192Tyr His Asp Ala Gln Gln Ala Gln Lys Asp Asn Arg Leu Tyr Glu Ala50 55 60cgt att ctc aaa cgc gcc aat ccc caa tta caa aat gcg gtg cat ctt240Arg Ile Leu Lys Arg Ala Asn Pro Gln Leu Gln Asn Ala Val His Leu65 70 75 80gcc att ctc gct ccc aat gct gaa ctg ata ggc tat aac aat caa ttt288Ala Ile Leu Ala Pro Asn Ala Glu Leu Ile Gly Tyr Asn Asn Gln Phe85 90 95agc ggt aga gcc agt caa tat gtt gcg ccg ggt acc gtt tct tcc atg336Ser Gly Arg Ala Ser Gln Tyr Val Ala Pro Gly Thr Val Ser Ser Met100 105 110ttc tcc ccc gcc gct tat ttg act gaa ctt tat cgt gaa gca cgc aat384Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Arg Asn115 120 125tta cac gca agt gac tcc gtt tat tat ctg gat acc cgc cgc cca gat432
Leu His Ala Ser Asp Ser Val Tyr Tyr Leu Asp Thr Arg Arg Pro Asp130 135 140ctc aaa tca atg gcg ctc agt cag caa aat atg gat ata gaa tta tcc480Leu Lys Ser Met Ala Leu Ser Gln Gln Asn Met Asp Ile Glu Leu Ser145 150 155 160aca ctc tct ttg tcc aat gag ctg tta ttg gaa agc att aaa act gaa528Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu Glu Ser Ile Lys Thr Glu165 170 175tct aaa ctg gaa aac tat act aaa gtg atg gaa atg ctc tcc act ttc576Ser Lys Leu Glu Asn Tyr Thr Lys Val Met Glu Met Leu Ser Thr Phe180 185 190cgt cct tcc ggc gca acg cct tat cat gat gct tat gaa aat gtg cgt624Arg Pro Ser Gly Ala Thr Pro Tyr His Asp Ala Tyr Glu Asn Val Arg195 200 205gaa gtt atc cag cta caa gat cct gga ctt gag caa ctc aat gca tca672Glu Val Ile Gln Leu Gln Asp Pro Gly Leu Glu Gln Leu Asn Ala Ser210 215 220ccg gca att gcc ggg ttg atg cat caa gcc tcc cta ttg ggt att aac720Pro Ala Ile Ala Gly Leu Met His Gln Ala Ser Leu Leu Gly Ile Asn225 230 235 240gct tca atc tcg cct gag cta ttt aat att ctg acg gag gag att acc768Ala Ser Ile Ser Pro Glu Leu Phe Asn Ile Leu Thr Glu Glu Ile Thr245 250 255gaa ggt aat gct gag gaa ctt tat aag aaa aat ttt ggt aat atc gaa816Glu Gly Asn Ala Glu Glu Leu Tyr Lys Lys Asn Phe Gly Asn Ile Glu260 265 270ccg gcc tca ttg gct atg ccg gaa tac ctt aaa cgt tat tat aat tta864Pro Ala Ser Leu Ala Met Pro Glu Tyr Leu Lys Arg Tyr Tyr Asn Leu275 280 285agc gat gaa gaa ctt agt cag ttt att ggt aaa gcc agc aat ttt ggt912Ser Asp Glu Glu Leu Ser Gln Phe Ile Gly Lys Ala Ser Asn Phe Gly290 295 300caa cag gaa tat agt aat aac caa ctt att act ccg gta gtc aac agc960
Gln Gln Glu Tyr Ser Asn Asn Gln Leu Ile Thr Pro Val Val Asn Ser305 310 315 320agt gat ggc acg gtt aag gta tat cgg atc acc cgc gaa tat aca acc1008Ser Asp Gly Thr Val Lys Val Tyr Arg Ile Thr Arg Glu Tyr Thr Thr325 330 335aat gct tat caa atg gat gtg gag cta ttt ccc ttc ggt ggt gag aat1056Asn Ala Tyr Gln Met Asp Val Glu Leu Phe Pro Phe Gly Gly Glu Asn340 345 350tat cgg tta gat tat aaa ttc aaa aat ttt tat aat gcc tct tat tta1104Tyr Arg Leu Asp Tyr Lys Phe Lys Asn Phe Tyr Asn Ala Ser Tyr Leu355 360 365tcc atc aag tta aat gat aaa aga gaa ctt gtt cga act gaa ggc gct1152Ser Ile Lys Leu Asn Asp Lys Arg Glu Leu Val Arg Thr Glu Gly Ala370 375 380cct caa gtc aat ata gaa tac tcc gca aat atc aca tta aat acc gct1200Pro Gln Val Asn Ile Glu Tyr Ser Ala Asn Ile Thr Leu Asn Thr Ala385 390 395 400gat atc agt caa cct ttt gaa att ggc ctg aca cga gta ctt cct tcc1248Asp Ile Ser Gln Pro Phe Glu Ile Gly Leu Thr Arg Val Leu Pro Ser405 410 415ggt tct tgg gca tat gcc gcc gca aaa ttt acc gtt gaa gag tat aac1296Gly Ser Trp Ala Tyr Ala Ala Ala Lys Phe Thr Val Glu Glu Tyr Asn420 425 430caa tac tct ttt ctg cta aaa ctt aac aag gct att cgt cta tca cgt1344Gln Tyr Ser Phe Leu Leu Lys Leu Asn Lys Ala Ile Arg Leu Ser Arg435 440 445gcg aca gaa ttg tca ccc acg att ctg gaa ggc att gtg cgc agt gtt1392Ala Thr Glu Leu Ser Pro Thr Ile Leu Glu Gly Ile Val Arg Ser Val450 455 460aat cta caa ctg gat atc aac aca gac gta tta ggt aaa gtt ttt ctg1440Asn Leu Gln Leu Asp Ile Asn Thr Asp Val Leu Gly Lys Val Phe Leu465 470 475 480act aaa tat tat atg cag cgt tat gct att cat gct gaa act gcc ctg1488
Thr Lys Tyr Tyr Met Gln Arg Tyr Ala Ile His Ala Glu Thr Ala Leu485 490 495ata cta tgc aac gcg cct att tca caa cgt tca tat gat aat caa cct1536Ile Leu Cys Asn Ala Pro Ile Ser Gln Arg Ser Tyr Asp Asn Gln Pro500 505 510agc caa ttt gat cgc ctg ttt aat acg cca tta ctg aac gga caa tat1584Ser Gln Phe Asp Arg Leu Phe Asn Thr Pro Leu Leu Asn Gly Gln Tyr515 520 525ttt tct acc ggc gat gag gag att gat tta aat tca ggt agc acc ggc1632Phe Ser Thr Gly Asp Glu Glu Ile Asp Leu Asn Ser Gly Ser Thr Gly530 535 540gat tgg cga aaa acc ata ctt aag cgt gca ttt aat att gat gat gtc1680Asp Trp Arg Lys Thr Ile Leu Lys Arg Ala Phe Asn Ile Asp Asp Val545 550 555 560tcg ctc ttc cgc ctg ctt aaa att acc gac cat gat aat aaa gat gga1728Ser Leu Phe Arg Leu Leu Lys Ile Thr Asp His Asp Asn Lys Asp Gly565 570 575aaa att aaa aat aac cta aag aat ctt tcc aat tta tat att gga aaa1776Lys Ile Lys Asn Asn Leu Lys Asn Leu Ser Asn Leu Tyr Ile Gly Lys580 585 590tta ctg gca gat att cat caa tta acc att gat gaa ctg gat tta tta1824Leu Leu Ala Asp Ile His Gln Leu Thr Ile Asp Glu Leu Asp Leu Leu595 600 605ctg att gcc gta ggt gaa gga aaa act aat tta tcc gct atc agt gat1872Leu Ile Ala Val Gly Glu Gly Lys Thr Asn Leu Ser Ala Ile Ser Asp610 615 620aag caa ttg gct acc ctg atc aga aaa ctc aat act att acc agc tgg1920Lys Gln Leu Ala Thr Leu Ile Arg Lys Leu Asn Thr Ile Thr Ser Trp625 630 635 640cta cat aca cag aag tgg agt gta ttc cag cta ttt atc atg acc tcc1968Leu His Thr Gln Lys Trp Ser Val Phe Gln Leu Phe Ile Met Thr Ser645 650 655acc agc tat aac aaa acg cta acg cct gaa att aag aat ttg ctg gat2016
Thr Ser Tyr Asn Lys Thr Leu Thr Pro Glu Ile Lys Asn Leu Leu Asp660 665 670acc gtc tac cac ggt tta caa ggt ttt gat aaa gac aaa gca gat ttg2064Thr Val Tyr His Gly Leu Gln Gly Phe Asp Lys Asp Lys Ala Asp Leu675 680 685cta cat gtc atg gcg ccc tat att gcg gcc acc ttg caa tta tca tcg2112Leu His Val Met Ala Pro Tyr Ile Ala Ala Thr Leu Gln Leu Ser Ser690 695 700gaa aat gtc gcc cac tcg gta ctc ctt tgg gca gat aag tta cag ccc2160Glu Asn Val Ala His Ser Val Leu Leu Trp Ala Asp Lys Leu Gln Pro705 710 715 720ggc gac ggc gca atg aca gca gaa aaa ttc tgg gac tgg ttg aat act2208Gly Asp Gly Ala Met Thr Ala Glu Lys Phe Trp Asp Trp Leu Asn Thr725 730 735aag tat acg ccg ggt tca tcg gaa gcc gta gaa acg cag gaa cat atc2256Lys Tyr Thr Pro Gly Ser Ser Glu Ala Val Glu Thr Gln Glu His Ile740 745 750gtt cag tat tgt cag gct ctg gca caa ttg gaa atg gtt tac cat tcc2304Val Gln Tyr Cys Gln Ala Leu Ala Gln Leu Glu Met Val Tyr His Ser755 760 765acc ggc atc aac gaa aac gcc ttc cgt cta ttt gtg aca aaa cca gag2352Thr Gly Ile Asn Glu Asn Ala Phe Arg Leu Phe Val Thr Lys Pro Glu770 775 780atg ttt ggc gct gca act gga gca gcg ccc gcg cat gat gcc ctt tca2400Met Phe Gly Ala Ala Thr Gly Ala Ala Pro Ala His Asp Ala Leu Ser785 790 795 800ctg att atg ctg aca cgt ttt gcg gat tgg gtg aac gca cta ggc gaa2448Leu Ile Met Leu Thr Arg Phe Ala Asp Trp Val Asn Ala Leu Gly Glu805 810 815aaa gcg tcc tcg gtg cta gcg gca ttt gaa gct aac tcg tta acg gca2496Lys Ala Ser Ser Val Leu Ala Ala Phe Glu Ala Asn Ser Leu Thr Ala820 825 830gaa caa ctg gct gat gcc atg aat ctt gat gct aat ttg ctg ttg caa2544
Glu Gln Leu Ala Asp Ala Met Asn Leu Asp Ala Asn Leu Leu Leu Gln835 840 845gcc agt att caa gca caa aat cat caa cat ctt ccc cca gta act cca2592Ala Ser Ile Gln Ala Gln Asn His Gln His Leu Pro Pro Val Thr Pro850 855 860gaa aat gcg ttc tcc tgt tgg aca tct atc aat act atc ctg caa tgg2640Glu Asn Ala Phe Ser Cys Trp Thr Ser Ile Asn Thr Ile Leu Gln Trp865 870 875 880gtt aat gtc gca caa caa ttg aat gtc gcc cca cag ggc gtt tcc gct2688Val Asn Val Ala Gln Gln Leu Asn Val Ala Pro Gln Gly Val Ser Ala885 890 895ttg gtc ggg ctg gat tat att caa tca atg aaa gag aca ccg acc tat2736Leu Val Gly Leu Asp Tyr Ile Gln Ser Met Lys Glu Thr Pro Thr Tyr900 905 910gcc cag tgg gaa aac gcg gca ggc gta tta acc gcc ggg ttg aat tca2784Ala Gln Trp Glu Asn Ala Ala Gly Val Leu Thr Ala Gly Leu Asn Ser915 920 925caa cag gct aat aca tta cac gct ttt ctg gat gaa tct cgc agt gcc2832Gln Gln Ala Asn Thr Leu His Ala Phe Leu Asp Glu Ser Arg Ser Ala930 935 940gca tta agc acc tac tat atc cgt caa gtc gcc aag gca gcg gcg gct2880Ala Leu Ser Thr Tyr Tyr Ile Arg Gln Val Ala Lys Ala Ala Ala Ala945 950 955 960att aaa agc cgt gat gac ttg tat caa tac tta ctg att gat aat cag2928Ile Lys Ser Arg Asp Asp Leu Tyr Gln Tyr Leu Leu Ile Asp Asn Gln965 970 975gtt tct gcg gca ata aaa acc acc cgg atc gcc gaa gcc att gcc agt2976Val Ser Ala Ala Ile Lys Thr Thr Arg Ile Ala Glu Ala Ile Ala Ser980 985 990att caa ctg tac gtc aac cgg gca ttg gaa aat gtg gaa gaa aat gcc3024Ile Gln Leu Tyr Val Asn Arg Ala Leu Glu Asn Val Glu Glu Asn Ala995 10001005aat tcg ggg gtt atc agc cgc caa ttc ttt atc gac tgg gac aaa 3069
Asn Ser Gly Val Ile Ser Arg Gln Phe Phe Ile Asp Trp Asp Lys1010 1015 1020tac aat aaa cgc tac agc act tgg gcg ggt gtt tct caa tta gtt3114Tyr Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Gln Leu Val1025 1030 1035tac tac ccg gaa aac tat att gat ccg acc atg cgt atc gga caa3159Tyr Tyr Pro Glu Asn Tyr Ile Asp Pro Thr Met Arg Ile Gly Gln1040 1045 1050acc aaa atg atg gac gca tta ctg caa tcc gtc agc caa agc caa3204Thr Lys Met Met Asp Ala Leu Leu Gln Ser Val Ser Gln Ser Gln1055 1060 1065tta aac gcc gat acc gtc gaa gat gcc ttt atg tct tat ctg aca3249Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Met Ser Tyr Leu Thr1070 1075 1080tcg ttt gaa caa gtg gct aat ctt aaa gtt att agc gca tat cac3294Ser Phe Glu Gln Val Ala Asn Leu Lys Val Ile Ser Ala Tyr His1085 1090 1095gat aat att aat aac gat caa ggg ctg acc tat ttt atc gga ctc3339Asp Asn Ile Asn Asn Asp Gln Gly Leu Thr Tyr Phe Ile Gly Leu1100 1105 1110agt gaa act gat gcc ggt gaa tat tat tgg cgc agt gtc gat cac3384Ser Glu Thr Asp Ala Gly Glu Tyr Tyr Trp Arg Ser Val Asp His1115 1120 1125agt aaa ttc aac gac ggt aaa ttc gcg gct aat gcc tgg agt gaa3429Ser Lys Phe Asn Asp Gly Lys Phe Ala Ala Asn Ala Trp Ser Glu1130 1135 1140tgg cat aaa att gat tgt cca att aac cct tat aaa agc act atc3474Trp His Lys Ile Asp Cys Pro Ile Asn Pro Tyr Lys Ser ThrIle1145 1150 1155cgt cca gtg ata tat aaa tcc cgc ctg tat ctg ctc tgg ttg gaa3519Arg Pro Val Ile Tyr Lys Ser Arg Leu Tyr Leu Leu Trp Leu Glu1160 1165 1170caa aag gag atc acc aaa cag aca gga aat agt aaa gat ggc tat3564
Gln Lys Glu Ile Thr Lys Gln Thr Gly Asn Ser Lys Asp Gly Tyr1175 1180 1185caa act gaa acg gat tat cgt tat gaa cta aaa ttg gcg cat atc3609Gln Thr Glu Thr Asp Tyr Arg Tyr Glu Leu Lys Leu Ala His Ile1190 1195 1200cgc tat gat ggc act tgg aat acg cca atc acc ttt gat gtc aat3654Arg Tyr Asp Gly Thr Trp Asn Thr Pro Ile Thr Phe Asp Val Asn1205 1210 1215aaa aaa ata tcc gag cta aaa ctg gaa aaa aat aga gcg ccc gga3699Lys Lys Ile Ser Glu Leu Lys Leu Glu Lys Asn Arg Ala Pro Gly1220 1225 1230ctc tat tgt gcc ggt tat caa ggt gaa gat acg ttg ctg gtg atg3744Leu Tyr Cys Ala Gly Tyr Gln Gly Glu Asp Thr Leu Leu Val Met1235 1240 1245ttt tat aac caa caa gac aca cta gat agt tat aaa aac gct tca3789Phe Tyr Asn Gln Gln Asp Thr Leu Asp Ser Tyr Lys Asn Ala Ser1250 1255 1260atg caa gga cta tat atc ttt gct gat atg gca tcc aaa gat atg3834Met Gln Gly Leu Tyr Ile Phe Ala Asp Met Ala Ser Lys Asp Met1265 1270 1275acc cca gaa cag agc aat gtt tat cgg gat aat agc tat caa caa3879Thr Pro Glu Gln Ser Asn Val Tyr Arg Asp Asn Ser Tyr Gln Gln1280 1285 1290ttt gat acc aat aat gtc aga aga gtg aat aac cgc tat gca gag3924Phe Asp Thr Asn Asn Val Arg Arg Val Asn Asn Arg Tyr Ala Glu1295 1300 1305gat tat gag att cct tcc tcg gta agt agc cgt aaa gac tat ggt3969Asp Tyr Glu Ile Pro Ser Ser Val Ser Ser Arg Lys Asp Tyr Gly1310 1315 1320tgg gga gat tat tac ctc agc atg gta tat aac gga gat att cca4014Trp Gly Asp Tyr Tyr Leu Ser Met Val Tyr Asn Gly Asp Ile Pro1325 1330 1335act atc aat tac aaa gcc gca tca agt gat tta aaa atc tat atc4059
Thr Ile Asn Tyr Lys Ala Ala Ser Ser Asp Leu Lys Ile Tyr Ile1340 1345 1350tca cca aaa tta aga att att cat aat gga tat gaa gga cag aag4104Ser Pro Lys Leu Arg Ile Ile His Asn Gly Tyr Glu Gly Gln Lys1355 1360 1365cgc aat caa tgc aat ctg atg aat aaa tat ggc aaa cta ggt gat4149Arg Asn Gln Cys Asn Leu Met Asn Lys Tyr Gly Lys Leu Gly Asp1370 1375 1380aaa ttt att gtt tat act agc ttg ggg gtc aat cca aat aac tcg4194Lys Phe Ile Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn Ser1385 1390 1395tca aat aag ctc atg ttt tac ccc gtc tat caa tat agc gga aac4239Ser Asn Lys Leu Met Phe Tyr Pro Val Tyr Gln Tyr Ser Gly Asn1400 1405 1410acc agt gga ctc aat caa ggg aga cta cta ttc cac cgt gac acc4284Thr Ser Gly Leu Asn Gln Gly Arg Leu Leu Phe His Arg Asp Thr1415 1420 1425act tat cca tct aaa gta gaa gct tgg att cct gga gca aaa cgt4329Thr Tyr Pro Ser Lys Val Glu Ala Trp Ile Pro Gly Ala Lys Arg1430 1435 1440tct cta acc aac caa aat gcc gcc att ggt gat gat tat gct aca4374Ser Leu Thr Asn Gln Asn Ala Ala Ile Gly Asp Asp Tyr Ala Thr1445 1450 1455gac tct ctg aat aaa ccg gat gat ctt aag caa tat atc ttt atg4419Asp Ser Leu Asn Lys Pro Asp Asp Leu Lys Gln Tyr Ile Phe Met1460 1465 1470act gac agt aaa ggg act gct act gat gtc tca ggc cca gta gag4464Thr Asp Ser Lys Gly Thr Ala Thr Asp Val Ser Gly Pro Val Glu1475 1480 1485att aat act gca att tct cca gca aaa gtt cag ata ata gtc aaa4509Ile Asn Thr Ala Ile Ser Pro Ala Lys Val Gln Ile Ile Val Lys1490 1495 1500gcg ggt ggc aag gag caa act ttt acc gca gat aaa gat gtc tcc4554
Ala Gly Gly Lys Glu Gln Thr Phe Thr Ala Asp Lys Asp Val Ser1505 1510 1515att cag cca tca cct agc ttt gat gaa atg aat tat caa ttt aat4599Ile Gln Pro Ser Pro Ser Phe Asp Glu Met Asn Tyr Gln Phe Asn1520 1525 1530gcc ctt gaa ata gac ggt tct ggt ctg aat ttt att aac aac tca4644Ala Leu Glu Ile Asp Gly Ser Gly Leu Asn Phe Ile Asn Asn Ser1535 1540 1545gcc agt att gat gtt act ttt acc gca ttt gcg gag gat ggc cgc4689Ala Ser Ile Asp Val Thr Phe Thr Ala Phe Ala Glu Asp Gly Arg1550 1555 1560aaa ctg ggt tat gaa agt ttc agt att cct gtt acc ctc aag gta4734Lys Leu Gly Tyr Glu Ser Phe Ser Ile Pro Val Thr Leu Lys Val1565 1570 1575agt acc gat aat gcc ctg acc ctg cac cat aat gaa aat ggt gcg4779Ser Thr Asp Asn Ala Leu Thr Leu His His Asn Glu Asn Gly Ala1580 1585 1590caa tat atg caa tgg caa tcc tat cgt acc cgc ctg aat act cta4824Gln Tyr Met Gln Trp Gln Ser Tyr Arg Thr Arg Leu Asn Thr Leu1595 1600 1605ttt gcc cgc cag ttg gtt gca cgc gcc acc acc gga atc gat aca4869Phe Ala Arg Gln Leu Val Ala Arg Ala Thr Thr Gly Ile Asp Thr1610 1615 1620att ctg agt atg gaa act cag aat att cag gaa ccg cag tta ggc4914Ile Leu Ser Met Glu Thr Gln Asn Ile Gln Glu Pro Gln Leu Gly1625 1630 1635aaa ggt ttc tat gct acg ttc gtg ata cct ccc tat aac cta tca4959Lys Gly Phe Tyr Ala Thr Phe Val Ile Pro Pro Tyr Asn Leu Ser1640 1645 1650act cat ggt gat gaa cgt tgg ttt aag ctt tat atc aaa cat gtt5004Thr His Gly Asp Glu Arg Trp Phe Lys Leu Tyr Ile Lys His Val1655 1660 1665gtt gat aat aat tca cat att atc tat tca ggc cag cta aca gat5049
Val Asp Asn Asn Ser His Ile Ile Tyr Ser Gly Gln Leu Thr Asp1670 1675 1680aca aat ata aac atc aca tta ttt att cct ctt gat gat gtc cca5094Thr Asn Ile Asn Ile Thr Leu Phe Ile Pro Leu Asp Asp Val Pro1685 1690 1695ttg aat caa gat tat cac gcc aag gtt tat atg acc ttc aag aaa5139Leu Asn Gln Asp Tyr His Ala Lys Val Tyr Met Thr Phe Lys Lys1700 1705 1710tca cca tca gat ggt acc tgg tgg ggc cct cac ttt gtt aga gat5184Ser Pro Ser Asp Gly Thr Trp Trp Gly Pro His Phe Val Arg Asp1715 1720 1725gat aaa gga ata gta aca ata aac cct aaa tcc att ttg acc cat5229Asp Lys Gly Ile Val Thr Ile Asn Pro Lys Ser Ile Leu Thr His1730 1735 1740ttt gag agc gtc aat gtc ctg aat aat att agt agc gaa cca atg5274Phe Glu Ser Val Asn Val Leu Asn Asn Ile Ser Ser Glu Pro Met1745 1750 1755gat ttc agc ggc gct aac agc ctc tat ttc tgg gaa ctg ttc tac5319Asp Phe Ser Gly Ala Asn Ser Leu Tyr Phe Trp Glu Leu Phe Tyr1760 1765 1770tat acc ccg atg ctg gtt gct caa cgt ttg ctg cat gaa cag aac5364Tyr Thr Pro Met Leu Val Ala Gln Arg Leu Leu His Glu Gln Asn1775 1780 1785ttc gat gaa gcc aac cgt tgg ctg aaa tat gtc tgg agt cca tcc5409Phe Asp Glu Ala Asn Arg Trp Leu Lys Tyr Val Trp Ser Pro Ser1790 1795 1800ggt tat att gtc cac ggc cag att cag aac tac cag tgg aac gtc5454Gly Tyr Ile Val His Gly Gln Ile Gln Asn Tyr Gln Trp Asn Val1805 1810 1815cgc ccg tta ctg gaa gac acc agt tgg aac agt gat cct ttg gat5499Arg Pro Leu Leu Glu Asp Thr Ser Trp Asn Ser Asp Pro Leu Asp1820 1825 1830tcc gtc gat cct gac gcg gta gca cag cac gat cca atg cac tac5544
Ser Val Asp Pro Asp Ala Val Ala Gln His Asp Pro Met His Tyr1835 1840 1845aaa gtt tca act ttt atg cgt acc ttg gat cta ttg ata gca cgc5589Lys Val Ser Thr Phe Met Arg Thr Leu Asp Leu Leu Ile Ala Arg1850 1855 1860ggc gac cat gct tat cgc caa ctg gaa cga gat aca ctc aac gaa5634Gly Asp His Ala Tyr Arg Gln Leu Glu Arg Asp Thr Leu Asn Glu1865 1870 1875gcg aag atg tgg tat atg caa gcg ctg cat cta tta ggt gac aaa5679Ala Lys Met Trp Tyr Met Gln Ala Leu His Leu Leu Gly Asp Lys1880 1885 1890cct tat cta ccg ctg agt acg aca tgg agt gat cca cga cta gac5724Pro Tyr Leu Pro Leu Ser Thr Thr Trp Ser Asp Pro Arg Leu Asp1895 1900 1905aga gcc gcg gat atc act acc caa aat gct cac gac agc gca ata 5769Arg Ala Ala Asp Ile Thr Thr Gln Asn Ala His Asp Ser Ala Ile1910 1915 1920gtc gct ctg cgg cag aat ata cct aca ccg gca cct tta tca ttg5814Val Ala Leu Arg Gln Asn Ile Pro Thr Pro Ala Pro Leu Ser Leu1925 1930 1935cgc agc gct aat acc ctg act gat ctc ttc ctg ccg caa atc aat5859Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gln Ile Asn1940 1945 1950gaa gtg atg atg aat tac tgg cag aca tta gct cag aga gta tac5904Glu Val Met Met Asn Tyr Trp Gln Thr Leu Ala Gln Arg Val Tyr1955 1960 1965aat ctg cgt cat aac ctc tct atc gac ggc cag ccg tta tat ctg5949Asn Leu Arg His Asn Leu Ser Ile Asp Gly Gln Pro Leu Tyr Leu1970 1975 1980cca atc tat gcc aca ccg gcc gat ccg aaa gcg tta ctc agc gcc5994Pro Ile Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala1985 1990 1995gcc gtt gcc act tct caa ggt gga ggc aag cta ccg gaa tca ttt6039
Ala Val Ala Thr Ser Gln Gly Gly Gly Lys Leu Pro Glu Ser Phe2000 2005 2010atg tcc ctg tgg cgt ttc ccg cac atg ctg gaa aat gcg cgc ggc6084Met Ser Leu Trp Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly2015 2020 2025atg gtt agc cag ctc acc cag ttc ggc tcc acg tta caa aat att6129Met Val Ser Gln Leu Thr Gln Phe Gly Ser Thr Leu Gln Asn Ile2030 2035 2040atc gaa cgt cag gac gcg gaa gcg ctc aat gcg tta tta caa aat6174Ile Glu Arg Gln Asp Ala Glu Ala Leu Asn Ala Leu Leu Gln Asn2045 2050 2055cag gcc gcc gag ctg ata ttg act aac ctg agc att cag gac aaa6219Gln Ala Ala Glu Leu Ile Leu Thr Asn Leu Ser Ile Gln Asp Lys2060 2065 2070acc att gaa gaa ttg gat gcc gag aaa acg gtg ttg gaa aaa tcc6264Thr Ile Glu Glu Leu Asp Ala Glu Lys Thr Val Leu Glu Lys Ser2075 2080 2085aaa gcg gga gca caa tcg cgc ttt gat agc tac ggc aaa ctg tac6309Lys Ala Gly Ala Gln Ser Arg Phe Asp Ser Tyr Gly Lys Leu Tyr2090 2095 2100gat gag aat atc aac gcc ggt gaa aac caa gcc atg acg cta cga6354Asp Glu Asn Ile Asn Ala Gly Glu Asn Gln Ala Met Thr Leu Arg2105 2110 2115gcg tcc gcc gcc ggg ctt acc acg gca gtt cag gca tcc cgt ctg6399Ala Ser Ala Ala Gly Leu Thr Thr Ala Val Gln Ala Ser Arg Leu2120 2125 2130gcc ggt gcg gcg gct gat ctg gtg cct aac atc ttc ggc ttt gcc6444Ala Gly Ala Ala Ala Asp Leu Val Pro Asn Ile Phe Gly Phe Ala2135 2140 2145ggt ggc ggc agc cgt tgg ggg gct atc gct gag gcg aca ggt tat6489Gly Gly Gly Ser Arg Trp Gly Ala Ile Ala Glu Ala Thr Gly Tyr2150 2155 2160gtg atg gaa ttc tcc gcg aat gtt atg aac acc gaa gcg gat aaa6534
Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala Asp Lys2165 2170 2175att agc caa tct gaa acc tac cgt cgt cgc cgt cag gag tgg gag6579Ile Ser Gln Ser Glu Thr Tyr Arg Arg Arg Arg Gln Glu Trp Glu2180 2185 2190atc cag cgg aat aat gcc gaa gcg gaa ttg aag caa atc gat gct6624Ile Gln Arg Asn Asn Ala Glu Ala Glu Leu Lys Gln Ile Asp Ala2195 2200 2205cag ctc aaa tca ctc gct gta cgc cgc gaa gcc gcc gta ttg cag6669Gln Leu Lys Ser Leu Ala Val Arg Arg Glu Ala Ala Val Leu Gln2210 2215 2220aaa acc agt ctg aaa acc caa caa gaa cag acc caa tct caa ttg6714Lys Thr Ser Leu Lys Thr Gln Gln Glu Gln Thr Gln Ser Gln Leu2225 2230 2235gcc ttc ctg caa cgt aag ttc agc aat cag gcg tta tac aac tgg6759Ala Phe Leu Gln Arg Lys Phe Ser Asn Gln Ala Leu Tyr Asn Trp2240 2245 2250ctg cgt ggt cga ctg gcg gcg att tac ttc cag ttc tac gat ttg6804Leu Arg Gly Arg Leu Ala Ala Ile Tyr Phe Gln Phe Tyr Asp Leu2255 2260 2265gcc gtc gcg cgt tgc ctg atg gca gaa caa gct tac cgt tgg gaa6849Ala Val Ala Arg Cys Leu Met Ala Glu Gln Ala Tyr Arg Trp Glu2270 2275 2280ctc aat gat gac tct gcc cgc ttc att aaa ccg ggc gcc tgg cag6894Leu Asn Asp Asp Ser Ala Arg Phe Ile Lys Pro Gly Ala Trp Gln2285 2290 2295gga acc tat gcc ggt ctg ctt gca ggt gaa acc ttg atg ctg agt6939Gly Thr Tyr Ala Gly Leu Leu Ala Gly Glu Thr Leu Met Leu Ser2300 2305 2310ctg gca caa atg gaa gac gct cat ctg aaa cgc gat aaa cgc gca6984Leu Ala Gln Met Glu Asp Ala His Leu Lys Arg Asp Lys Arg Ala2315 2320 2325tta gag gtt gaa cgc aca gta tcg ctg gcc gaa gtt tat gca gga7029
Leu Glu Val Glu Arg Thr Val Ser Leu Ala Glu Val Tyr Ala Gly2330 2335 2340tta cca aaa gat aac ggt cca ttt tcc ctg gct cag gaa att gac7074Leu Pro Lys Asp Asn Gly Pro Phe Ser Leu Ala Gln Glu Ile Asp2345 2350 2355aag ctg gtg agt caa ggt tca ggc agt gcc ggc agt ggt aat aat7119Lys Leu Val Ser Gln Gly Ser Gly Ser Ala Gly Ser Gly Asn Asn2360 2365 2370aat ttg gcg ttc ggc gcc ggc acg gac act aaa acc tct ttg cag7164Asn Leu Ala Phe Gly Ala Gly Thr Asp Thr Lys Thr Ser Leu Gln2375 2380 2385gca tca gtt tca ttc gct gat ttg aaa att cgt gaa gat tac ccg7209Ala Ser Val Ser Phe Ala Asp Leu Lys Ile Arg Glu Asp Tyr Pro2390 2395 2400gca tcg ctt ggc aaa att cga cgt atc aaa cag atc agc gtc act7254Ala Ser Leu Gly Lys Ile Arg Arg Ile Lys Gln Ile Ser Val Thr2405 2410 2415ttg ccc gcg cta ctg gga ccg tat cag gat gta cag gca ata ttg7299Leu Pro Ala Leu Leu Gly Pro Tyr Gln Asp Val Gln Ala Ile Leu2420 2425 2430tct tac ggc gat aaa gcc gga tta gct aac ggc tgt gaa gcg ctg7344Ser Tyr Gly Asp Lys Ala Gly Leu Ala Asn Gly Cys Glu Ala Leu2435 2440 2445gca gtt tct cac ggt atg aat gac agc ggc caa ttc cag ctc gat7389Ala Val Ser His Gly Met Asn Asp Ser Gly Gln Phe Gln Leu Asp2450 2455 2460ttc aac gat ggc aaa ttc ctg cca ttc gaa ggc atc gcc att gat7434Phe Asn Asp Gly Lys Phe Leu Pro Phe Glu Gly Ile Ala Ile Asp2465 2470 2475caa ggc acg ctg aca ctg agc ttc cca aat gca tct atg ccg gag7479Gln Gly Thr Leu Thr Leu Ser Phe Pro Asn Ala Ser Met Pro Glu2480 2485 2490aaa ggt aaa caa gcc act atg tta aaa acc ctg aac gat atc att7524
Lys Gly Lys Gln Ala Thr Met Leu Lys Thr Leu Asn Asp Ile Ile249525002505ttg cat att cgc tac acc att aaa taa7551Leu His Ile Arg Tyr Thr Ile Lys25102515<210>22<211>4431<212>DNA<213>发光光杆状菌<220>
<221>外显子<222>(1)..(4431)<400>22atg cag aat tca caa aca ttc agt gtt acc gag ctg tca tta ccc aaa 48Met Gln Asn Ser Gln Thr Phe Ser Val Thr Glu Leu Ser Leu Pro Lys1 5 10 15ggc ggc ggc gct att acc ggt atg ggt gaa gca tta aca cca gcc ggg 96Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Ala Leu Thr Pro Ala Gly20 25 30ccg gat ggt atg gcc gcc tta tcc ctg cca tta ccc att tcc gcc ggg 144Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Leu Pro Ile Ser Ala Gly35 40 45cgt ggt tac gca ccc tcg ctc act ctg aat tac aac agt gga acc ggt 192Arg Gly Tyr Ala Pro Ser Leu Thr Leu Asn Tyr Ash Ser Gly Thr Gly50 55 60aac agc cca ttt ggt ctc ggt tgg gac tgc ggc gtc atg gca att cgt 240Asn Ser Pro Phe Gly Leu Gly Trp Asp Cys Gly Val Met Ala Ile Arg65 70 75 80cgt cgc acc agt acc ggc gta ccg aat tac gat gaa acc gat act ttt 288Arg Arg Thr Ser Thr Gly Val Pro Asn Tyr Asp Glu Thr Asp Thr Phe85 90 95ctg ggg ccg gaa ggt gaa gtg ttg gtc gta gca tta aat gag gca ggt 336
Leu Gly Pro Glu Gly Glu Val Leu Val Val Ala Leu Asn Glu Ala Gly100 105 110caa gct gat atc cgc agt gaa tcc tca ttg cag ggc atc aat ttg ggt 384Gln Ala Asp Ile Arg Ser Glu Ser Ser Leu Gln Gly Ile Asn Leu Gly115 120 125gcg acc ttc acc gtt acc tgt tat cgc tcc cgc cta gaa agc cac ttt 432Ala Thr Phe Thr Val Thr Cys Tyr Arg Ser Arg Leu Glu Ser His Phe130 135 140aac cgg ttg gaa tac tgg caa ccc caa aca acc ggc gca acc gat ttc 480Asn Arg Leu Glu Tyr Trp Gln Pro Gln Thr Thr Gly Ala Thr Asp Phe145 150 155 160tgg ctg ata tac agc ccc gac gga cag gtc cat tta ctg ggc aaa aat 528Trp Leu Ile Tyr Ser Pro Asp Gly Gln Val His Leu Leu Gly Lys Asn165 170 175cct cag gca cgt atc agc aat cca ctc aat gtt aac caa aca gcg caa 576Pro Gln Ala Arg Ile Ser Asn Pro Leu Asn Val Asn Gln Thr Ala Gln180 185 190tgg ctg ttg gaa gcc tcg ata tca tcc cac agc gaa cag att tat tat 624Trp Leu Leu Glu Ala Ser Ile Ser Ser His Ser Glu Gln Ile Tyr Tyr195 200 205caa tat cgc gct gaa gat gaa gca ggt tgt gaa acc gac gag cta gca 672Gln Tyr Arg Ala Glu Asp Glu Ala Gly Cys Glu Thr Asp Glu Leu Ala210 215 220gcc cac ccc agc gca acc gtt cag cgc tac ctg caa aca gta cat tac 720Ala His Pro Ser Ala Thr Val Gln Arg Tyr Leu Gln Thr Val His Tyr225 230 235 240ggg aac ctg acc gcc agc gac gtt ttt cct aca cta aac gga gat gac 768Gly Asn Leu Thr Ala Ser Asp Val Phe Pro Thr Leu Asn Gly Asp Asp245 250 255cca ctt aaa tct ggc tgg atg ttc tgt tta gta ttt gac tac ggt gag 816Pro Leu Lys Ser Gly Trp Met Phe Cys Leu Val Phe Asp Tyr Gly Glu260 265 270cgc aaa aac agc tta tct gaa atg ccg ctg ttt aaa gcc aca ggc aat 864
Arg Lys Asn Ser Leu Ser Glu Met Pro Leu Phe Lys Ala Thr Gly Asn275 280 285tgg ctt tgc cga aaa gac cgt ttt tcc cgt tat gag tac ggt ttt gaa 912Trp Leu Cys Arg Lys Asp Arg Phe Ser Arg Tyr Glu Tyr Gly Phe Glu290 295 300ttg cgt act cgc cgc tta tgc cgc caa ata ctg atg ttt cac cgt cta 960Leu Arg Thr Arg Arg Leu Cys Arg Gln Ile Leu Met Phe His Arg Leu305 310 315 320caa acc cta tct ggt cag gca aag ggg gat gat gaa cct gcg cta gtg1008Gln Thr Leu Ser Gly Gln Ala Lys Gly Asp Asp Glu Pro Ala Leu Val325 330 335tcg cgt ctg ata ctg gat tat gac gaa aac gcg atg gtc agt acg ctc1056Ser Arg Leu Ile Leu Asp Tyr Asp Glu Asn Ala Met Val Ser Thr Leu340 345 350gtt tct gtc cgc cgg gta ggc cat gag gac aac aac acg gtt acc gcg1104Val Ser Val Arg Arg Val Gly His Glu Asp Asn Asn Thr Val Thr Ala355 360 365ctg cca cca ctg gaa ctg gcc tat cag cct ttt gag cca gaa caa acc1152Leu Pro Pro Leu Glu Leu Ala Tyr Gln Pro Phe Glu Pro Glu Gln Thr370 375 380gca ctc tgg caa tca atg gat gta ctg gca aat ttc aac acc att cag1200Ala Leu Trp Gln Ser Met Asp Val Leu Ala Asn Phe Asn Thr Ile Gln385 390 395 400cgc tgg caa ctg ctt gac ctg aaa gga gaa ggc gtg ccc ggc att ctc1248Arg Trp Gln Leu Leu Asp Leu Lys Gly Glu Gly Val Pro Gly Ile Leu405 410 415tat cag gat aga aat ggc tgg tgg tat cga tct gcc caa cgt cag gcc1296Tyr Gln Asp Arg Asn Gly Trp Trp Tyr Arg Ser Ala Gln Arg Gln Ala420 425 430ggg gaa gag atg aat gcg gtc acc tgg ggg aaa atg caa ctc ctt ccc1344Gly Glu Glu Met Asn Ala Val Thr Trp Gly Lys Met Gln Leu Leu Pro435 440 445atc aca cca gct gtg cag gat aac gcc tca ctg atg gat att aac ggt1392
Ile Thr Pro Ala Val Gln Asp Asn Ala Ser Leu Met Asp Ile Asn Gly450 455 460gac ggg caa ctg gac tgg gtg att acc ggg ccg ggg cta agg ggc tat1440Asp Gly Gln Leu Asp Trp Val Ile Thr Gly Pro Gly Leu Arg Gly Tyr465 470 475 480cac agc caa cac ccg gat ggc agt tgg acg cgt ttt acg cca tta cat1488His Ser Gln His Pro Asp Gly Ser Trp Thr Arg Phe Thr Pro Leu His485 490 495gcc ctg ccg ata gaa tat tct cat cct cgc gct caa ctt gcc gat tta1536Ala Leu Pro Ile Glu Tyr Ser His Pro Arg Ala Gln Leu Ala Asp Leu500 505 510atg gga gcc ggg ctg tcc gat tta gtg cta att ggt ccc aaa agt gtg1584Met Gly Ala Gly Leu Ser Asp Leu Val Leu Ile Gly Pro Lys Ser Val515 520 525cgc tta tat gtc aat aac cgt gat ggt ttt acc gaa ggg cgg gat gtg1632Arg Leu Tyr Val Asn Asn Arg Asp Gly Phe Thr Glu Gly Arg Asp Val530 535 540gtg caa tcc ggt gat atc acc ctg ccg cta ccg ggc gcc gat gcc cgt1680Val Gln Ser Gly Asp Ile Thr Leu Pro Leu Pro Gly Ala Asp Ala Arg545 550 555 560aag tta gtg gca ttt agt gac gta ctg ggt tca ggc caa gca cat ctg1728Lys Leu Val Ala Phe Ser Asp Val Leu Gly Ser Gly Gln Ala His Leu565 570 575gtt gaa gtt agt gca act caa gtc acc tgc tgg ccg aat ctg ggg cat1776Val Glu Val Ser Ala Thr Gln Val Thr Cys Trp Pro Asn Leu Gly His580 585 590ggc cgt ttt ggt cag cca atc gta ttg ccg gga ttc agc caa tct gcc1824Gly Arg Phe Gly Gln Pro Ile Val Leu Pro Gly Phe Ser Gln Ser Ala595 600 605gcc agt ttt aat cct gat cga gtt cat ctg gcc gat ttg gat ggg agc1872Ala Ser Phe Asn Pro Asp Arg Val His Leu Ala Asp Leu Asp Gly Ser610 615 620ggc cct gcc gat ttg att tat gtt cat gct gac cgt ctg gat att ttc1920
Gly Pro Ala Asp Leu Ile Tyr Val His Ala Asp Arg Leu Asp Ile Phe625 630 635 640agc aat gaa agt ggc aac ggt ttt gca aaa cca ttc aca ctc tct ttt1968Ser Asn Glu Ser Gly Asn Gly Phe Ala Lys Pro Phe Thr Leu Ser Phe645 650 655cct gac ggc ctg cgt ttt gat gat acc tgc cag ttg caa gta gcc gat2016Pro Asp Gly Leu Arg Phe Asp Asp Thr Cys Gln Leu Gln Val Ala Asp660 665 670gta caa ggg tta ggc gtt gtc agc ctg atc cta agc gta ccg cat atg2064Val Gln Gly Leu Gly Val Val Ser Leu Ile Leu Ser Val Pro His Met675 680 685gcg cca cat cat tgg cgc tgc gat ctg acc aac gcg aaa ccg tgg tta2112Ala Pro His His Trp Arg Cys Asp Leu Thr Asn Ala Lys Pro Trp Leu690 695 700ctc agt gaa acg aac aac aat atg ggg gcc aat cac acc ttg cat tac2160Leu Ser Glu Thr Asn Asn Asn Met Gly Ala Asn His Thr Leu His Tyr705 710 715 720cgt agc tct gtc cag ttc tgg ctg gat gaa aaa gct gcg gca ttg gct2208Arg Ser Ser Val Gln Phe Trp Leu Asp Glu Lys Ala Ala Ala Leu Ala725 730 735acc gga caa aca ccg gtc tgt tac ctg ccc ttc ccg gtc cat acc ctt2256Thr Gly Gln Thr Pro Val Cys Tyr Leu Pro Phe Pro Val His Thr Leu740 745 750tgg caa aca gaa acc gag gat gaa atc agc ggc aat aag tta gtg acc2304Trp Gln Thr Glu Thr Glu Asp Glu Ile Ser Gly Asn Lys Leu Val Thr755 760 765acg tta cgt tat gct cac ggc gct tgg gat gga cgt gaa cgg gaa ttt2352Thr Leu Arg Tyr Ala His Gly Ala Trp Asp Gly Arg Glu Arg Glu Phe770 775 780cgt ggc ttt ggt tat gtt gag cag aca gac agc cat caa ctc gct caa2400Arg Gly Phe Gly Tyr Val Glu Gln Thr Asp Ser His Gln Leu Ala Gln785 790 79 5800ggc aat gcg ccg gaa cgt aca cca ccg gca ctc acc aaa agc tgg tat2448
Gly Asn Ala Pro Glu Arg Thr Pro Pro Ala Leu Thr Lys Ser Trp Tyr805 810 815gcc acc gga tta cct gcg gta gat aat gcg tta tcc gcc ggg tat tgg2496Ala Thr Gly Leu Pro Ala Val Asp Asn Ala Leu Ser Ala Gly Tyr Trp820 825 830cgt ggc gat aag caa gct ttc gcc ggt ttt acg cca cgt ttt act ctc2544Arg Gly Asp Lys Gln Ala Phe Ala Gly Phe Thr Pro Arg Phe Thr Leu835 840 845tgg aaa gag ggc aaa gat gtt cca ctg aca ccg gaa gat gac cat aat2592Trp Lys Glu Gly Lys Asp Val Pro Leu Thr Pro Glu Asp Asp His Asn850 855 860cta tac tgg tta aac cgg gcg cta aaa ggt cag cca ctg cgt agt gaa2640Leu Tyr Trp Leu Asn Arg Ala Leu Lys Gly Gln Pro Leu Arg Ser Glu865 870 875 880ctc tac ggg ctg gat ggc agc gca cag caa cag atc ccc tat aca gtg2688Leu Tyr Gly Leu Asp Gly Ser Ala Gln Gln Gln Ile Pro Tyr Thr Val885 890 895act gaa tcc cgt cca cag gtg cgc caa tta caa gat ggc gcc acc gtt2736Thr Glu Ser Arg Pro Gln Val Arg Gln Leu Gln Asp Gly Ala Thr Val900 905 910tcc ccg gtg ctc tgg gcc tca gtc gtg gaa agc cgt agt tat cac tac2784Ser Pro Val Leu Trp Ala Ser Val Val Glu Ser Arg Ser Tyr His Tyr915 920 925gaa cgt att atc agt gat ccc cag tgc aat cag gat atc acg ttg tcc2832Glu Arg Ile Ile Ser Asp Pro Gln Cys Asn Gln Asp Ile Thr Leu Ser930 935 940agt gac cta ttc ggg caa cca ctg aaa cag gtt tcc gta caa tat ccc2880Ser Asp Leu Phe Gly Gln Pro Leu Lys Gln Val Ser Val Gln Tyr Pro945 950 955 960cgc cgc aac aaa cca aca acc aat ccg tat ccc gat acc cta ccg gat2928Arg Arg Asn Lys Pro Thr Thr Asn Pro Tyr Pro Asp Thr Leu Pro Asp965 970 975acg ctg ttt gcc agc agt tat gac gat caa caa cag cta ttg cga tta2976
Thr Leu Phe Ala Ser Ser Tyr Asp Asp Gln Gln Gln Leu Leu Arg Leu980 985 990acc tgc cga caa tcc agt tgg cac cat ctt att ggt aat gag cta aga 3024Thr Cys Arg Gln Ser Ser Trp His His Leu Ile Gly Asn Glu Leu Arg995 10001005gtg ttg gga tta ccg gat ggc aca cgc agt gat gcc ttt act tac3069Val Leu Gly Leu Pro Asp Gly Thr Arg Ser Asp Ala Phe Thr Tyr1010 1015 1020gat gcc aaa cag gta cct gtc gat ggc tta aat ctg gaa acc ctg3114Asp Ala Lys Gln Val Pro Val Asp Gly Leu Asn Leu Glu Thr Leu1025 1030 1035tgt gct gaa aat agc ctg att gcc gat gat aaa cct cgc gaa tac3159Cys Ala Glu Asn Ser Leu Ile Ala Asp Asp Lys Pro Arg Glu Tyr1040 1045 1050ctc aat cag caa cga acg ttc tat acc gac ggg aaa aac caa aca3204Leu Asn Gln Gln Arg Thr Phe Tyr Thr Asp Gly Lys Asn Gln Thr1055 1060 1065ccg ctg aaa aca ccg aca cga caa gcg tta atc gcc ttt acc gaa3249Pro Leu Lys Thr Pro Thr Arg Gln Ala Leu Ile Ala Phe Thr Glu1070 1075 1080acg gcg gta tta acg gaa tct ctg tta tcc gcg ttt gat ggc ggt3294Thr Ala Val Leu Thr Glu Ser Leu Leu Ser Ala Phe Asp Gly Gly1085 1090 1095att acg cca gac gaa tta ccg gga ata ctg aca cag gcc gga tac3339Ile Thr Pro Asp Glu Leu Pro Gly Ile Leu Thr Gln Ala Gly Tyr1100 1105 1110caa caa gag cct tat ctg ttt cca cgc acc ggc gaa aac aaa gtt3384Gln Gln Glu Pro Tyr Leu Phe Pro Arg Thr Gly Glu Asn Lys Val1115 1120 1125tgg gta gcg cgt caa ggc tat acc gat tac ggg acg gaa gca caa3429Trp Val Ala Arg Gln Gly Tyr Thr Asp Tyr Gly Thr Glu Ala Gln1130 1135 1140ttt tgg cgt cct gtc gca caa cgt aac agc ctg tta acc ggg aaa3474
Phe Trp Arg Pro Val Ala Gln Arg Asn Ser Leu Leu Thr Gly Lys1145 1150 1155atg acg tta aaa tgg gat act cac tat tgt gtc atc acc caa acc3519Met Thr Leu Lys Trp Asp Thr His Tyr Cys Val Ile Thr Gln Thr1160 1165 1170caa gat gct gcc ggc ctc acc gtc tca gcc aat tat gac tgg cgt3564Gln Asp Ala Ala Gly Leu Thr Val Ser Ala Asn Tyr Asp Trp Arg1175 1180 1185ttt ctc aca cca acg caa ctg act gac atc aac gat aat gtg cat3609Phe Leu Thr Pro Thr Gln Leu Thr Asp Ile Asn Asp Asn Val His1190 1195 1200ctc atc acc ttg gat gct ctg gga cgc cct gtc acg caa cgt ttc3654Leu Ile Thr Leu Asp Ala Leu Gly Arg Pro Val Thr Gln Arg Phe1205 1210 1215tgg ggg atc gaa agc ggt gtg gca aca ggt tac tct tca tca gaa3699Trp Gly Ile Glu Ser Gly Val Ala Thr Gly Tyr Ser Ser Ser Glu1220 1225 1230gaa aaa cca ttc tct cca cca aac gat atc gat acc gct att aat3744Glu Lys Pro Phe Ser Pro Pro Asn Asp Ile Asp Thr Ala Ile Asn1235 1240 1245cta acc gga cca ctc cct gtc gca cag tgt ctg gtc tat gca ccg3789Leu Thr Gly Pro Leu Pro Val Ala Gln Cys Leu Val Tyr Ala Pro1250 1255 1260gac agt tgg atg cca cta ttc agt caa gaa acc ttc aac aca tta3834Asp Ser Trp Met Pro Leu Phe Ser Gln Glu Thr Phe Asn Thr Leu1265 1270 1275acg cag gaa gag cag gag acg ctg cgt gat tca cgt att atc acg3879Thr Gln Glu Glu Gln Glu Thr Leu Arg Asp Ser Arg Ile Ile Thr1280 1285 1290gaa gat tgg cgt att tgc gca ctg act cgc cgc cgt tgg cta caa3924Glu Asp Trp Arg Ile Cys Ala Leu Thr Arg Arg Arg Trp Leu Gln1295 1300 1305agt caa aag atc agt aca cca tta gtt aaa ctg tta acc aac agc3969
Ser Gln Lys Ile Ser Thr Pro Leu Val Lys Leu Leu Thr Asn Ser1310 1315 1320att ggt tta cct ccc cat aac ctt acg ctg acc aca gac cgt tat4014Ile Gly Leu Pro Pro His Asn Leu Thr Leu Thr Thr Asp Arg Tyr1325 1330 1335gac cgc gac tct gag cag caa att cgc caa caa gtc gca ttt agt4059Asp Arg Asp Ser Glu Gln Gln Ile Arg Gln Gln Val Ala Phe Ser1340 1345 1350gat ggt ttt ggc cgt ctg cta caa gcg tct gta cga cat gag gca4104Asp Gly Phe Gly Arg Leu Leu Gln Ala Ser Val Arg His Glu Ala1355 1360 1365ggc gaa gcc tgg caa cgt aac caa gac ggt tct ctg gtg aca aaa4149Gly Glu Ala Trp Gln Arg Asn Gln Asp Gly Ser Leu Val Thr Lys1370 1375 1380gtg gag aat acc aaa acg cgt tgg gcg gtc acg gga cgc acc gaa4194Val Glu Asn Thr Lys Thr Arg Trp Ala Val Thr Gly Arg Thr Glu1385 1390 1395tat gat aat aaa ggg caa acg ata cgc act tat cag ccc tat ttc4239Tyr Asp Asn Lys Gly Gln Thr Ile Arg Thr Tyr Gln Pro Tyr Phe1400 1405 1410ctc aac gac tgg cga tat gtc agt gat gac agc gcc aga aaa gaa4284Leu Asn Asp Trp Arg Tyr Val Ser Asp Asp Ser Ala Arg Lys Glu1415 1420 1425gcc tat gcg gat act cat att tat gat cca att ggg cga gaa atc4329Ala Tyr Ala Asp Thr His Ile Tyr Asp Pro Ile Gly Arg Glu Ile1430 1435 1440cgg gtt att act gca aaa ggc tgg ctg cgc caa agc caa tat ttc4374Arg Val Ile Thr Ala Lys Gly Trp Leu Arg Gln Ser Gln Tyr Phe1445 1450 1455ccg tgg ttt acc gtg agt gag gat gag aat gat acg gcc gct gat4419Pro Trp Phe Thr Val Ser Glu Asp Glu Asn Asp Thr Ala Ala Asp1460 1465 1470gcg ctg gtg taa 4431
Ala Leu Val1475<210>23<211>74<212>DNA<213>人工序列<220>
<223>用于从质粒pBC-AS4扩增TcdB1序列的正向引物<400>23atatagtcga cgaattttaa tctactagta aaaaggagat aaccatgcag aattcacaaa 60cattcagtgt tacc 74<210>24<211>57<212>DNA<213>人工序列<220>
<223>用于从质粒pBC-AS4扩增TcdB1序列的反向引物.
<400>24ataatacgat cgtttctcga gtcattacac cagcgcatca gcggccgtat cattctc57<210>25<211>3132<212>DNA<213>发光光杆状菌<220>
<221>外显子<222>(1)..(3132)<400>25atg agt ccg tct gag act act ctt tat act caa acc cca aca gtc agc 48Met Ser Pro Ser Glu Thr Thr Leu Tyr Thr Gln Thr Pro Thr Val Ser1 5 10 15
gtg tta gat aat cgc ggt ctg tcc att cgt gat att ggt ttt cac cgt 96Val Leu Asp Asn Arg Gly Leu Ser Ile Arg Asp Ile Gly Phe His Arg20 25 30att gta atc ggg ggg gat act gac acc cgc gtc acc cgt cac cag tat144Ile Val Ile Gly Gly Asp Thr Asp Thr Arg Val Thr Arg His Gln Tyr35 40 45gat gcc cgt gga cac ctg aac tac agt att gac cca cgc ttg tat gat192Asp Ala Arg Gly His Leu Asn Tyr Ser Ile Asp Pro Arg Leu Tyr Asp50 55 60gca aag cag gct gat aac tca gta aag cct aat ttt gtc tgg cag cat240Ala Lys Gln Ala Asp Asn Ser Val Lys Pro Asn Phe Val Trp Gln His65 70 75 80gat ctg gcc ggt cat gcc ctg cgg aca gag agt gtc gat gct ggt cgt288Asp Leu Ala Gly His Ala Leu Arg Thr Glu Ser Val Asp Ala Gly Arg85 90 95act gtt gca ttg aat gat att gaa ggt cgt tcg gta atg aca atg aat336Thr Val Ala Leu Asn Asp Ile Glu Gly Arg Ser Val Met Thr Met Asn100 105 110gcg acc ggt gtt cgt cag acc cgt cgc tat gaa ggc aac acc ttg ccc384Ala Thr Gly Val Arg Gln Thr Arg Arg Tyr Glu Gly Asn Thr Leu Pro115 120 125ggt cgc ttg tta tct gtg agc gag caa gtt ttc aac caa gag agt gct432Gly Arg Leu Leu Ser Val Ser Glu Gln Val Phe Asn Gln Glu Ser Ala130 135 140aaa gtg aca gag cgc ttt atc tgg gct ggg aat aca acc tcg gag aaa480Lys Val Thr Glu Arg Phe Ile Trp Ala Gly Asn Thr Thr Ser Glu Lys145 150 155 160gag tat aac ctc tcc ggt ctg tgt ata cgc cac tac gac aca gcg gga528Glu Tyr Asn Leu Ser Gly Leu Cys Ile Arg His Tyr Asp Thr Ala Gly165 170 175gtg acc cgg ttg atg agt cag tca ctg gcg ggc gcc atg cta tcc caa576Val Thr Arg Leu Met Ser Gln Ser Leu Ala Gly Ala Met Leu Ser Gln180 185 190
tct cac caa ttg ctg gcg gaa ggg cag gag gct aac tgg agc ggt gac 624Ser His Gln Leu Leu Ala Glu Gly Gln Glu Ala Asn Trp Ser Gly Asp195 200 205gac gaa act gtc tgg cag gga atg ctg gca agt gag gtc tat acg aca 672Asp Glu Thr Val Trp Gln Gly Met Leu Ala Ser Glu Val Tyr Thr Thr210 215 220caa agt acc act aat gcc atc ggg gct tta ctg acc caa acc gat gcg 720Gln Ser Thr Thr Asn Ala Ile Gly Ala Leu Leu Thr Gln Thr Asp Ala225 230 235 240aaa ggc aat att cag cgt ctg gct tat gac att gcc ggt cag tta aaa 768Lys Gly Asn Ile Gln Arg Leu Ala Tyr Asp Ile Ala Gly Gln Leu Lys245 250 255ggg agt tgg ttg acg gtg aaa ggc cag agt gaa cag gtg att gtt aag 816Gly Ser Trp Leu Thr Val Lys Gly Gln Ser Glu Gln Val Ile Val Lys260 265 270tcc ctg agc tgg tca gcc gca ggt cat aaa ttg cgt gaa gag cac ggt 864Ser Leu Ser Trp Ser Ala Ala Gly His Lys Leu Arg Glu Glu His Gly275 280 285aac ggc gtg gtt acg gag tac agt tat gag ccg gaa act caa cgt ctg 912Asn Gly Val Val Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gln Arg Leu290 295 300ata ggt atc acc acc cgg cgt gcc gaa ggg agt caa tca gga gcc aga 960Ile Gly Ile Thr Thr Arg Arg Ala Glu Gly Ser Gln Ser Gly Ala Arg305 310 315 320gta ttg cag gat cta cgc tat aag tat gat ccg gtg ggg aat gtt atc1008Val Leu Gln Asp Leu Arg Tyr Lys Tyr Asp Pro Val Gly Asn Val Ile325 330 335agt atc cat aat gat gcc gaa gct acc cgc ttt tgg cgt aat cag aaa1056Ser Ile His Asn Asp Ala Glu Ala Thr Arg Phe Trp Arg Asn Gln Lys340 345 350gtg gag ccg gag aat cgc tat gtt tat gat tct ctg tat cag ctt atg1104Val Glu Pro Glu Asn Arg Tyr Val Tyr Asp Ser Leu Tyr Gln Leu Met355 360 365
agt gcg aca ggg cgt gaa atg gct aat atc ggt cag caa agc aac caa1152Ser Ala Thr Gly Arg Glu Met Ala Asn Ile Gly Gln Gln Ser Asn Gln370 375 380ctt ccc tca ccc gtt ata cct gtt cct act gac gac agc act tat acc1200Leu Pro Ser Pro Val Ile Pro Val Pro Thr Asp Asp Ser Thr Tyr Thr385 390 395 400aat tac ctt cgt acc tat act tat gac cgt ggc ggt aat ttg gtt caa1248Asn Tyr Leu Arg Thr Tyr Thr Tyr Asp Arg Gly Gly Asn Leu Val Gln405 410 415atc cga cac agt tca ccc gcg act caa aat agt tac acc aca gat atc1296Ile Arg His Ser Ser Pro Ala Thr Gln Asn Ser Tyr Thr Thr Asp Ile420 425 430acc gtt tca agc cgc agt aac cgg gcg gta ttg agt aca tta acg aca1344Thr Val Ser Ser Arg Ser Asn Arg Ala Val Leu Ser Thr Leu Thr Thr435 440 445gat cca acc cga gtg gat gcg cta ttt gat tcc ggc ggt cat cag aag1392Asp Pro Thr Arg Val Asp Ala Leu Phe Asp Ser Gly Gly His Gln Lys450 455 460atg tta ata ccg ggg caa aat ctg gat tgg aat att cgg ggt gaa ttg1440Met Leu Ile Pro Gly Gln Asn Leu Asp Trp Asn Ile Arg Gly Glu Leu465 470 475 480caa cga gtc aca ccg gtg agc cgt gaa aat agc agt gac agt gaa tgg1488Gln Arg Val Thr Pro Val Ser Arg Glu Asn Ser Ser Asp Ser Glu Trp485 490 495tat cgc tat agc agt gat ggc atg cgg ctg cta aaa gtg agt gaa cag1536Tyr Arg Tyr Ser Ser Asp Gly Met Arg Leu Leu Lys Val Ser Glu Gln500 505 510cag acg ggc aac agt act caa gta caa cgg gtg act tat ctg ccg gga1584Gln Thr Gly Asn Ser Thr Gln Val Gln Arg Val Thr Tyr Leu Pro Gly515 520 525tta gag cta cgg aca act ggg gtt gca gat aaa aca acc gaa gat ttg1632Leu Glu Leu Arg Thr Thr Gly Val Ala Asp Lys Thr Thr Glu Asp Leu530 535 540
cag gtg att acg gta ggt gaa gcg ggt cgc gca cag gta agg gta ttg1680Gln Val Ile Thr Val Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu545 550 555 560cac tgg gaa agt ggt aag ccg aca gat att gac aac aat cag gtg cgc1728His Trp Glu Ser Gly Lys Pro Thr Asp Ile Asp Asn Asn Gln Val Arg565 570 575tac agc tac gat aat ctg ctt ggc tcc agc cag ctt gaa ctg gat agc1776Tyr Ser Tyr Asp Asn Leu Leu Gly Ser Ser Gln Leu Glu Leu Asp Ser580 585 590gaa ggg cag att ctc agt cag gaa gag tat tat ccg tat ggc ggt acg1824Glu Gly Gln Ile Leu Ser Gln Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr595 600 605gcg ata tgg gcg gcg aga aat cag aca gaa gcc agc tac aaa ttt att1872Ala Ile Trp Ala Ala Arg Asn Gln Thr Glu Ala Ser Tyr Lys Phe Ile610 615 620cgt tac tcc ggt aaa gag cgg gat gcc act gga ttg tat tat tac ggc1920Arg Tyr Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly625 630 635 640tac cgt tat tat caa cct tgg gtg ggt cga tgg ttg agt gct gat ccg1968Tyr Arg Tyr Tyr Gln Pro Trp Val Gly Arg Trp Leu Ser Ala Asp Pro645 650 655gcg gga acc gtg gat ggg ctg aat ttg tac cga atg gtg agg aat aac2016Ala Gly Thr Val Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn660 665 670ccc atc aca ttg act gac cat gac gga tta gca ccg tct cca aat aga2064Pro Ile Thr Leu Thr Asp His Asp Gly Leu Ala Pro Ser Pro Asn Arg675 680 685aat cga aat aca ttt tgg ttt gct tca ttt ttg ttt cgt aaa cct gat2112Asn Arg Asn Thr Phe Trp Phe Ala Ser Phe Leu Phe Arg Lys Pro Asp690 695 700gag gga atg tcc gcg tca atg aga cgg gga caa aaa att ggc aga gcc2160Glu Gly Met Ser Ala Ser Met Arg Arg Gly Gln Lys Ile Gly Arg Ala705 710 715 720
att gcc ggc ggg att gcg att ggc ggt ctt gcg gct acc att gcc gct2208Ile Ala Gly Gly Ile Ala Ile Gly Gly Leu Ala Ala Thr Ile Ala Ala725 730 735acg gct ggc gcg gct atc ccc gtc att ctg ggg gtt gcg gcc gta ggc2256Thr Ala Gly Ala Ala Ile Pro Val Ile Leu Gly Val Ala Ala Val Gly740 745 750gcg ggg att ggc gcg ttg atg gga tat aac gtc ggt agc ctg ctg gaa2304Ala Gly Ile Gly Ala Leu Met Gly Tyr Asn Val Gly Ser Leu Leu Glu755 760 765aaa ggc ggg gca tta ctt gct cga ctc gta cag ggg aaa tcg acg tta2352Lys Gly Gly Ala Leu Leu Ala Arg Leu Val Gln Gly Lys Ser Thr Leu770 775 780gta cag tcg gcg gct ggc gcg gct gcc gga gcg agt tca gcc gcg gct2400Val Gln Ser Ala Ala Gly Ala Ala Ala Gly Ala Ser Ser Ala Ala Ala785 790 795 800tat ggc gca cgg gca caa ggt gtc ggt gtt gca tca gcc gcc ggg gcg2448Tyr Gly Ala Arg Ala Gln Gly Val Gly Val Ala Ser Ala Ala Gly Ala805 810 815gta aca ggg gct gtg gga tca tgg ata aat aat gct gat cgg ggg att2496Val Thr Gly Ala Val Gly Ser Trp Ile Asn Asn Ala Asp Arg Gly Ile820 825 830ggc ggc gct att ggg gcc ggg agt gcg gta ggc acc att gat act atg2544Gly Gly Ala Ile Gly Ala Gly Ser Ala Val Gly Thr Ile Asp Thr Met835 840 845tta ggg act gcc tct acc ctt acc cat gaa gtc ggg gca gcg gcg ggt2592Leu Gly Thr Ala Ser Thr Leu Thr His Glu Val Gly Ala Ala Ala Gly850 855 860ggg gcg gcg ggt ggg atg atc acc ggt acg caa ggg agt act cgg gca2640Gly Ala Ala Gly Gly Met Ile Thr Gly Thr Gln Gly Ser Thr Arg Ala865 870 875 880ggt atc cat gcc ggt att ggc acc tat tat ggc tcc tgg att ggt ttt2688Gly Ile His Ala Gly Ile Gly Thr Tyr Tyr Gly Ser Trp Ile Gly Phe885 890 895
ggt tta gat gtc gct agt aac ccc gcc gga cat tta gcg aat tac gca2736Gly Leu Asp Val Ala Ser Asn Pro Ala Gly His Leu Ala Asn Tyr Ala900 905 910gtg ggt tat gcc gct ggt ttg ggt gct gaa atg gct gtc aac aga ata2784Val Gly Tyr Ala Ala Gly Leu Gly Ala Glu Met Ala Val Asn Arg Ile915 920 925atg ggt ggt gga ttt ttg agt agg ctc tta ggc cgg gtt gtc agc cca2832Met Gly Gly Gly Phe Leu Ser Arg Leu Leu Gly Arg Val Val Ser Pro930 935 940tat gcc gcc ggt tta gcc aga caa tta gta cat ttc agt gtc gcc aga2880Tyr Ala Ala Gly Leu Ala Arg Gln Leu Val His Phe Ser Val Ala Arg945 950 955 960cct gtc ttt gag ccg ata ttt agt gtt ctc ggc ggg ctt gtc ggt ggt2928Pro Val Phe Glu Pro Ile Phe Ser Val Leu Gly Gly Leu Val Gly Gly965 970 975att gga act ggc ctg cac aga gtg atg gga aga gag agt tgg att tcc2976Ile Gly Thr Gly Leu His Arg Val Met Gly Arg Glu Ser Trp Ile Ser980 985 990aga gcg tta agt gct gcc ggt agt ggt ata gat cat gtc gct ggc atg3024Arg Ala Leu Ser Ala Ala Gly Ser Gly Ile Asp His Val Ala Gly Met995 10001005att ggt aat cag atc aga ggc agg gtc ttg acc aca acc ggg atc3069Ile Gly Asn Gln Ile Arg Gly Arg Val Leu Thr Thr Thr Gly Ile101010151020gct aat gcg ata gac tat ggc acc agt gct gtg gga gcc gca cga3114Ala Asn Ala Ile Asp Tyr Gly Thr Ser Ala Val Gly Ala Ala Arg102510301035cga gtt ttt tct ttg taa3132Arg Val Phe Ser Leu1040<210>26<211>77
<212>DNA<213>人工序列<220>
<223>用于从pBC KS+载体扩增TccC1的正向引物<400>26gtcgacgcac tactagtaaa aaggagataa ccccatgagc ccgtctgaga ctactcttta60tactcaaacc ccaacag 77<210>27<211>57<212>DNA<213>人工序列<220>
<223>用于从pBC KS+载体扩增TccC1的反向引物<400>27cggccgcagt cctcgagtca gattaattac aaagaaaaaa ctcgtcgtgc ggctccc 57<210>28<211>84<212>DNA<213>人工序列<220>
<223>用于扩增XptA2的正向引物.
<400>28gtctagacgt gcgtcgacaa gaaggagata taccatgtat agcacggctg tattactcaa 60taaaatcagt cccactcgcg acgg84<210>29<211>64<212>DNA<213>人工序列<220>
<223>用于扩增XptA2的反向引物.
<400>29gctcgagatt aattaagaac gaatggtata gcggatatgc agaatgatat cgctcaggct60ctcc 64<210>30<211>81<212>DNA<213>人工序列<220>
<223>用于扩增XptC1的正向引物.
<400>30gtctagacgt gcgtcgacaa gaaggagata taccatgcag ggttcaacac ctttgaaact60tgaaataccg tcattgccct c 81<210>31<211>64<212>DNA<213>人工序列<220>
<223>用于扩增XptC1的反向引物.
<400>31gactcgagag cattaattat gctgtcattt caccggcagt gtcattttca tcttcattca60ccac 64<210>32<211>86<212>DNA<213>人工序列<220>
<223>用于扩增XptB1的正向引物.
<400>32gtctagacgt gcgtcgacaa gaaggagata taccatgaag aatttcgttc acagcaatac60gccatccgtc accgtactgg acaacc 86<210>33<211>66<212>DNA<213>人工序列<220>
<223>用于扩增XptB1的反向引物.
<400>33gctcgagcag attaattatg cttcggattc attatgacgt gcagaggcgt taaagaagaa60gttatt 66<210>34<211>2538<212>PRT<213>嗜线虫致病杆菌<400>34Met Tyr Ser Thr Ala Val Leu Leu Asn Lys Ile Ser Pro Thr Arg Asp1 5 10 15Gly Gln Thr Met Thr Leu Ala Asp Leu Gln Tyr Leu Ser Phe Ser Glu20 25 30Leu Arg Lys Ile Phe Asp Asp Gln Leu Ser Trp Gly Glu Ala Arg His35 40 45Leu Tyr His Glu Thr Ile Glu Gln Lys Lys Asn Asn Arg Leu Leu Glu50 55 60Ala Arg Ile Phe Thr Arg Ala Asn Pro Gln Leu Ser Gly Ala Ile Arg
65 70 75 80Leu Gly Ile Glu Arg Asp Ser Val Ser Arg Ser Tyr Asp Glu Met Phe85 90 95Gly Ala Arg Ser Ser Ser Phe Val Lys Pro Gly Ser Val Ala Ser Met100 105 110Phe Ser Pro Ala Gly Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asp115 120 125Leu His Phe Ser Ser Ser Ala Tyr His Leu Asp Asn Arg Arg Pro Asp130 135 140Leu Ala Asp Leu Thr Leu Ser Gln Ser Asn Met Asp Thr Glu Ile Ser145 150 155 160Thr Leu Thr Leu Ser Asn Glu Leu Leu Leu Glu His Ile Thr Arg Lys165 170 175Thr Gly Gly Asp Ser Asp Ala Leu Met Glu Ser Leu Ser Thr Tyr Arg180 185 190Gln Ala Ile Asp Thr Pro Tyr His Gln Pro Tyr Glu Thr Ile Arg Gln195 200 205Val Ile Met Thr His Asp Ser Thr Leu Ser Ala Leu Ser Arg Asn Pro210 215 220Glu Val Met Gly Gln Ala Glu Gly Ala Ser Leu Leu Ala Ile Leu Ala225 230 235 240Asn Ile Ser Pro Glu Leu Tyr Asn Ile Leu Thr Glu Glu Ile Thr Glu
245 250 255Lys Asn Ala Asp Ala Leu Phe Ala Gln Asn Phe Ser Glu Asn Ile Thr260 265 270Pro Glu Asn Phe Ala Ser Gln Ser Trp Ile Ala Lys Tyr Tyr Gly Leu275 280 285Glu Leu Ser Glu Val Gln Lys Tyr Leu Gly Met Leu Gln Asn Gly Tyr290 295 300Ser Asp Ser Thr Ser Ala Tyr Val Asp Asn Ile Ser Thr Gly Leu Val305 310 315 320Val Asn Asn Glu Ser Lys Leu Glu Ala Tyr Lys Ile Thr Arg Val Lys325 330 335Thr Asp Asp Tyr Asp Lys Asn Ile Asn Tyr Phe Asp Leu Met Tyr Glu340 345 350Gly Asn Asn Gln Phe Phe Ile Arg Ala Asn Phe Lys Val Ser Arg Glu355 360 365Phe Gly Ala Thr Leu Arg Lys Asn Ala Gly Pro Ser Gly Ile Val Gly370 375 380Ser Leu Ser Gly Pro Leu Ile Ala Asn Thr Asn Phe Lys Ser Asn Tyr385 390 395 400Leu Ser Asn Ile Ser Asp Ser Glu Tyr Lys Asn Gly Val Lys Ile Tyr405 410 415Ala Tyr Arg Tyr Thr Ser Ser Thr Ser Ala Thr Asn Gln Gly Gly Gly
420 425 430Ile Phe Thr Phe Glu Ser Tyr Pro Leu Thr Ile Phe Ala Leu Lys Leu435 440 445Asn Lys Ala Ile Arg Leu Cys Leu Thr Ser Gly Leu Ser Pro Asn Glu450 455 460Leu Gln Thr Ile Val Arg Ser Asp Asn Ala Gln Gly Ile Ile Asn Asp465 470 475 480Ser Val Leu Thr Lys Val Phe Tyr Thr Leu Phe Tyr Ser His Arg Tyr485 490 495Ala Leu Ser Phe Asp Asp Ala Gln Val Leu Asn Gly Ser Val Ile Asn500 505 510Gln Tyr Ala Asp Asp Asp Ser Val Ser His Phe Asn Arg Leu Phe Asn515 520 525Thr Pro Pro Leu Lys Gly Lys Ile Phe Glu Ala Asp Gly Asn Thr Val530 535 540Ser Ile Asp Pro Asp Glu Glu Gln Ser Thr Phe Ala Arg Ser Ala Leu545 550 555 560Met Arg Gly Leu Gly Val Asn Ser Gly Glu Leu Tyr Gln Leu Gly Lys565 570 575Leu Ala Gly Val Leu Asp Ala Gln Asn Thr Ile Thr Leu Ser Val Phe580 585 590Val Ile Ser Ser Leu Tyr Arg Leu Thr Leu Leu Ala Arg Val His Gln
595 600 605Leu Thr Val Asn Glu Leu Cys Met Leu Tyr Gly Leu Ser Pro Phe Asn610 615 620Gly Lys Thr Thr Ala Ser Leu Ser Ser Gly Glu Leu Pro Arg Leu Val625 630 635 640Ile Trp Leu Tyr Gln Val Thr Gln Trp Leu Thr Glu Ala Glu Ile Thr645 650 655Thr Glu Ala Ile Trp Leu Leu Cys Thr Pro Glu Phe Ser Gly Asn Ile660 665 670Ser Pro Glu Ile Ser Asn Leu Leu Asn Asn Leu Arg Pro Ser Ile Ser675 680 685Glu Asp Met Ala Gln Ser His Asn Arg Glu Leu Gln Ala Glu Ile Leu690 695 700Ala Pro Phe Ile Ala Ala Thr Leu His Leu Ala Ser Pro Asp Met Ala705 710 715 720Arg Tyr Ile Leu Leu Trp Thr Asp Asn Leu Arg Pro Gly Gly Leu Asp725 730 735Ile Ala Gly Phe Met Thr Leu Val Leu Lys Glu Ser Leu Asn Ala Asn740 745 750Glu Thr Thr Gln Leu Val Gln Phe Cys His Val Met Ala Gln Leu Ser755 760 765Leu Ser Val Gln Thr Leu Arg Leu Ser Glu Ala Glu Leu Ser Val Leu
770 775 780Val Ile Ser Gly Phe Ala Val Leu Gly Ala Lys Asn Gln Pro Ala Gly785 790 795 800Gln His Asn Ile Asp Thr Leu Phe Ser Leu Tyr Arg Phe His Gln Trp805 810 815Ile Asn Gly Leu Gly Asn Pro Gly Ser Asp Thr Leu Asp Met Leu Arg820 825 830Gln Gln Thr Leu Thr Ala Asp Arg Leu Ala Ser Val Met Gly Leu Asp835 840 845Ile Ser Met Val Thr Gln Ala Met Val Ser Ala Gly Val Asn Gln Leu850 855 860Gln Cys Trp Gln Asp Ile Asn Thr Val Leu Gln Trp Ile Asp Val Ala865 870 875 880Ser Ala Leu His Thr Met Pro Ser Val Ile Arg Thr Leu Val Asn Ile885 890 895Arg Tyr Val Thr Ala Leu Asn Lys Ala Glu Ser Asn Leu Pro Ser Trp900 905 910Asp Glu Trp Gln Thr Leu Ala Glu Asn Met Glu Ala Gly Leu Ser Thr915 920 925Gln Gln Ala Gln Thr Leu Ala Asp Tyr Thr Ala Glu Arg Leu Ser Ser930 935 940Val Leu Cys Asn Trp Phe Leu Ala Asn Ile Gln Pro Glu Gly Val Ser
945 950 955 960Leu His Ser Arg Asp Asp Leu Tyr Ser Tyr Phe Leu Ile Asp Asn Gln965 970 975Val Ser Ser Ala Ile Lys Thr Thr Arg Leu Ala Glu Ala Ile Ala Gly980 985 990Ile Gln Leu Tyr Ile Asn Arg Ala Leu Asn Arg Ile Glu Pro Asn Ala995 1000 1005Arg Ala Asp Val Ser Thr Arg Gln Phe Phe Thr Asp Trp Thr Val1010 1015 1020Asn Asn Arg Tyr Ser Thr Trp Gly Gly Val Ser Arg Leu Val Tyr1025 1030 1035Tyr Pro Glu Asn Tyr Ile Asp Pro Thr Gln Arg Ile Gly Gln Thr1040 1045 1050Arg Met Met Asp Glu Leu Leu Glu Asn Ile Ser Gln Ser Lys Leu1055 1060 1065Ser Arg Asp Thr Val Glu Asp Ala Phe Lys Thr Tyr Leu Thr Arg1070 1075 1080Phe Glu Thr Val Ala Asp Leu Lys Val Val Ser Ala Tyr His Asp1085 1090 1095Asn Val Asn Ser Asn Thr Gly Leu Thr Trp Phe Val Gly Gln Thr1100 1105 1110Arg Glu Asn Leu Pro Glu Tyr Tyr Trp Arg Asn Val Asp Ile Ser
1115 1120 1125Arg Met Gln Ala Gly Glu Leu Ala Ala Asn Ala Trp Lys Glu Trp1130 1135 1140Thr Lys Ile Asp Thr Ala Val Asn Pro Tyr Lys Asp Ala Ile Arg1145 1150 1155Pro Val Ile Phe Arg Glu Arg Leu His Leu Ile Trp Val Glu Lys1160 1165 1170Glu Glu Val Ala Lys Asn Gly Thr Asp Pro Val Glu Thr Tyr Asp1175 1180 1185Arg Phe Thr Leu Lys Leu Ala Phe Leu Arg His Asp Gly Ser Trp1190 1195 1200Ser Ala Pro Trp Ser Tyr Asp Ile Thr Thr Gln Val Glu Ala Val1205 1210 1215Thr Asp Lys Lys Pro Asp Thr Glu Arg Leu Ala Leu Ala Ala Ser1220 1225 1230Gly Phe Gln Gly Glu Asp Thr Leu Leu Val Phe Val Tyr Lys Thr1235 1240 1245Gly Lys Ser Tyr Ser Asp Phe Gly Gly Ser Asn Lys Asn Val Ala1250 1255 1260Gly Met Thr Ile Tyr Gly Asp Gly Ser Phe Lys Lys Met Glu Asn1265 1270 1275Thr Ala Leu Ser Arg Tyr Ser Gln Leu Lys Asn Thr Phe Asp Ile
1280 1285 1290Ile His Thr Gln Gly Asn Asp Leu Val Arg Lys Ala Ser Tyr Arg1295 1300 1305Phe Ala Gln Asp Phe Glu Val Pro Ala Ser Leu Asn Met Gly Ser1310 1315 1320Ala Ile Gly Asp Asp Ser Leu Thr Val Met Glu Asn Gly Asn Ile1325 1330 1335Pro Gln Ile Thr Ser Lys Tyr Ser Ser Asp Asn Leu Ala Ile Thr1340 1345 1350Leu His Asn Ala Ala Phe Thr Val Arg Tyr Asp Gly Ser Gly Asn1355 1360 1365Val Ile Arg Asn Lys Gln Ile Ser Ala Met Lys Leu Thr Gly Val1370 1375 1380Asp Gly Lys Ser Gln Tyr Gly Asn Ala Phe Ile Ile Ala Asn Thr1385 1390 1395Val Lys His Tyr Gly Gly Tyr Ser Asp Leu Gly Gly Pro Ile Thr1400 1405 1410Val Tyr Asn Lys Thr Lys Asn Tyr Ile Ala Ser Val Gln Gly His1415 1420 1425Leu Met Asn Ala Asp Tyr Thr Arg Arg Leu Ile Leu Thr Pro Val1430 1435 1440Glu Asn Asn Tyr Tyr Ala Arg Leu Phe Glu Phe Pro Phe Ser Pro
1445 1450 1455Asn Thr Ile Leu Asn Thr Val Phe Thr Val Gly Ser Asn Lys Thr1460 1465 1470Ser Asp Phe Lys Lys Cys Ser Tyr Ala Val Asp Gly Asn Asn Ser1475 1480 1485Gln Gly Phe Gln Ile Phe Ser Ser Tyr Gln Ser Ser Gly Trp Leu1490 1495 1500Asp Ile Asp Thr Gly Ile Asn Asn Thr Asp Ile Lys Ile Thr Val1505 1510 1515Met Ala Gly Ser Lys Thr His Thr Phe Thr Ala Ser Asp His Ile1520 1525 1530Ala Ser Leu Pro Ala Asn Ser Phe Asp Ala Met Pro Tyr Thr Phe1535 1540 1545Lys Pro Leu Glu Ile Asp Ala Ser Ser Leu Ala Phe Thr Asn Asn1550 1555 1560Ile Ala Pro Leu Asp Ile Val Phe Glu Thr Lys Ala Lys Asp Gly1565 1570 1575Arg Val Leu Gly Lys Ile Lys Gln Thr Leu Ser Val Lys Arg Val1580 1585 1590Asn Tyr Asn Pro Glu Asp Ile Leu Phe Leu Arg Glu Thr His Ser1595 1600 1605Gly Ala Gln Tyr Met Gln Leu Gly Val Tyr Arg Ile Arg Leu Asn
1610 1615 1620Thr Leu Leu Ala Ser Gln Leu Val Ser Arg Ala Asn Thr Gly Ile1625 1630 1635Asp Thr Ile Leu Thr Met Glu Thr Gln Arg Leu Pro Glu Pro Pro1640 1645 1650Leu Gly Glu Gly Phe Phe Ala Asn Phe Val Leu Pro Lys Tyr Asp1655 1660 1665Pro Ala Glu His Gly Asp Glu Arg Trp Phe Lys Ile His Ile Gly1670 1675 1680Asn Val Gly Gly Asn Thr Gly Arg Gln Pro Tyr Tyr Ser Gly Met1685 1690 1695Leu Ser Asp Thr Ser Glu Thr Ser Met Thr Leu Phe Val Pro Tyr1700 1705 1710A1a Glu Gly Tyr Tyr Met His Glu Gly Val Arg Leu Gly Val Gly1715 1720 1725Tyr Gln Lys Ile Thr Tyr Asp Asn Thr Trp Glu Ser Ala Phe Phe1730 1735 1740Tyr Phe Asp Glu Thr Lys Gln Gln Phe Val Leu Ile Asn Asp Ala1745 1750 1755Asp His Asp Ser Gly Met Thr Gln Gln Gly Ile Val Lys Asn Ile1760 1765 1770Lys Lys Tyr Lys Gly Phe Leu Asn Val Ser Ile Ala Thr Gly Tyr
1775 1780 1785Ser Ala Pro Met Asp Phe Asn Ser Ala Ser Ala Leu Tyr Tyr Trp1790 1795 1800Glu Leu Phe Tyr Tyr Thr Pro Met Met Cys Phe Gln Arg Leu Leu1805 1810 1815Gln Glu Lys Gln Phe Asp Glu Ala Thr Gln Trp Ile Asn Tyr Val1820 1825 1830Tyr Asn Pro Ala Gly Tyr Ile Val Asn Gly Glu Ile Ala Pro Trp1835 1840 1845Ile Trp Asn Cys Arg Pro Leu Glu Glu Thr Thr Ser Trp Asn Ala1850 1855 1860Asn Pro Leu Asp Ala Ile Asp Pro Asp Ala Val Ala Gln Asn Asp1865 1870 1875Pro Met His Tyr Lys Ile Ala Thr Phe Met Arg Leu Leu Asp Gln1880 1885 1890Leu Ile Leu Arg Gly Asp Met Ala Tyr Arg Glu Leu Thr Arg Asp1895 1900 1905Ala Leu Asn Glu Ala Lys Met Trp Tyr Val Arg Thr Leu Glu Leu1910 1915 1920Leu Gly Asp Glu Pro Glu Asp Tyr Gly Ser Gln Gln Trp Ala Ala1925 1930 1935Pro Ser Leu Ser Gly Ala Ala Ser Gln Thr Val Gln Ala Ala Tyr
1940 1945 1950Gln Gln Asp Leu Thr Met Leu Gly Arg Gly Gly Val Ser Lys Asn1955 1960 1965Leu Arg Thr Ala Asn Ser Leu Val Gly Leu Phe Leu Pro Glu Tyr1970 1975 1980Asn Pro Ala Leu Thr Asp Tyr Trp Gln Thr Leu Arg Leu Arg Leu1985 1990 1995Phe Asn Leu Arg His Asn Leu Ser Ile Asp Gly Gln Pro Leu Ser2000 2005 2010Leu Ala Ile Tyr Ala Glu Pro Thr Asp Pro Lys Ala Leu Leu Thr2015 2020 2025Ser Met Val Gln Ala Ser Gln Gly Gly Ser Ala Val Leu Pro Gly2030 2035 2040Thr Leu Ser Leu Tyr Arg Phe Pro Val Met Leu Glu Arg Thr Arg2045 2050 2055Asn Leu Val Ala Gln Leu Thr Gln Phe Gly Thr Ser Leu Leu Ser2060 2065 2070Met Ala Glu His Asp Asp Ala Asp Glu Leu Thr Thr Leu Leu Leu2075 2080 2085Gln Gln Gly Met Glu Leu Ala Thr Gln Ser Ile Arg Ile Gln Gln2090 2095 2100Arg Thr Val Asp Glu Val Asp Ala Asp Ile Ala Val Leu Ala Glu
2105 2110 2115Ser Arg Arg Ser Ala Gln Asn Arg Leu Glu Lys Tyr Gln Gln Leu2120 2125 2130Tyr Asp Glu Asp Ile Asn His Gly Glu Gln Arg Ala Met Ser Leu2135 2140 2145Leu Asp Ala Ala Ala Gly Gln Ser Leu Ala Gly Gln Val Leu Ser2150 2155 2160Ile Ala Glu Gly Val Ala Asp Leu Val Pro Asn Val Phe Gly Leu2165 2170 2175Ala Cys Gly Gly Ser Arg Trp Gly Ala Ala Leu Arg Ala Ser Ala2180 2185 2190Ser Val Met Ser Leu Ser Ala Thr Ala Ser Gln Tyr Ser Ala Asp2195 2200 2205Lys Ile Ser Arg Ser Glu Ala Tyr Arg Arg Arg Arg Gln Glu Trp2210 2215 2220Glu Ile Gln Arg Asp Asn Ala Asp Gly Glu Val Lys Gln Met Asp2225 2230 2235Ala Gln Leu Glu Ser Leu Lys Ile Arg Arg Glu Ala Ala Gln Met2240 2245 2250Gln Val Glu Tyr Gln Glu Thr Gln Gln Ala His Thr Gln Ala Gln2255 2260 2265Leu Glu Leu Leu Gln Arg Lys Phe Thr Asn Lys Ala Leu Tyr Ser
2270 2275 2280Trp Met Arg Gly Lys Leu Ser Ala Ile Tyr Tyr Gln Phe Phe Asp2285 2290 2295Leu Thr Gln Ser Phe Cys Leu Met Ala Gln Glu Ala Leu Arg Arg2300 2305 2310Glu Leu Thr Asp Asn Gly Val Thr Phe Ile Arg Gly Gly Ala Trp2315 2320 2325Asn Gly Thr Thr Ala Gly Leu Met Ala Gly Glu Thr Leu Leu Leu2330 2335 2340Asn Leu Ala Glu Met Glu Lys Val Trp Leu Glu Arg Asp Glu Arg2345 2350 2355Ala Leu Glu Val Thr Arg Thr Val Ser Leu Ala Gln Phe Tyr Gln2360 2365 2370Ala Leu Ser Ser Asp Asn Phe Asn Leu Thr Glu Lys Leu Thr Gln2375 2380 2385Phe Leu Arg Glu Gly Lys Gly Asn Val Gly Ala Ser Gly Asn Glu2390 2395 2400Leu Lys Leu Ser Asn Arg Gln Ile Glu Ala Ser Val Arg Leu Ser2405 2410 2415Asp Leu Lys Ile Phe Ser Asp Tyr Pro Glu Ser Leu Gly Asn Thr2420 2425 2430Arg Gln Leu Lys Gln Val Ser Val Thr Leu Pro Ala Leu Val Gly
2435 2440 2443Pro Tyr Glu Asp Ile Arg Ala Val Leu Asn Tyr Gly Gly Ser Ile2450 2455 2460Val Met Pro Arg Gly Cys Ser Ala Ile Ala Leu Ser His Gly Val2465 2470 2475Asn Asp Ser Gly Gln Phe Met Leu Asp Phe Asn Asp Ser Arg Tyr2480 2485 2490Leu Pro Phe Glu Gly Ile Ser Val Asn Asp Ser Gly Ser Leu Thr2495 2500 2505Leu Ser Phe Pro Asp Ala Thr Asp Arg Gln Lys Ala Leu Leu Glu2510 2515 2520Ser Leu Ser Asp Ile Ile Leu His Ile Arg Tyr Thr Ile Arg Ser2525 2530 2535<210>35<211>3300<212>DNA<213>类芽孢杆菌(Paenibacillus)DAS1529株<400>35atggtgtcaa caacagacaa cacggccggc gtattccggc tcggaaccga agaattaaca 60gaagcgctta agcagtccgg ttatcggacc gtctttgata ttgtatctga caatcttgcg 120gaatttcaga aaaacaatcc ggagattccc tcttctgacg cgaaggagat tcatcaatta 180gccgtccaga ggacagaaaa cttatgcatg ctttataagg cctggcagct gcacaatgat 240ccggttgtcc agagccttcc caaattatcc gcggataccg gcctgcaagg catgcgtgcc 300gcgttggagc ggagtcttgg aggcggagcc gattttggag acttgttccc ggagcgatcg 360
ccagagggct atgcggaagc ctcctctata cagtcgcttt tctcgccggg acgttacctg 420acggtgctgt ataaaattgc gcgggatctc cacgacccaa aagataaact gcatattgac 480aaccgccgtc cagatttgaa gtcgctgatc ctcaataatg acaatatgaa ccgagaggta 540tcttctctgg atatccttct ggatgtgctg cagcccgaag gctctgacac gctgacatcc 600ttgaaggata cctaccatcc gatgaccctt ccctatgatg acgaccttgc gcaaatcaat 660gccgtggcgg aggcgcgttc atctaatttg ctggggattt gggataccct gctggacacg 720cagcggactt ccatcctgca gaattccgcc gctgcccgcc ggataagcaa ggcgcggcac 780tcggcatacg ccaatcagaa agcctccaat gatgagccgg tattcatcac gggagaggaa 840atctacctgg aaaccggagg taaacggctt tttctggcgc ataaactcga gataggttca 900actattagcg ctaaaatcaa cattggaccg ccgcaagcgg ccgatatcgc gccggcaaag 960ttgcaactcg tatattacgg cagaggcggc agagggaact acttcctgcg cgtggcagac1020gatgtgtccc tcggtggaaa gctgctgacc aattgttatc tgaccagcga tgacggacag1080agcaacaata ttagcgggcc atactgccta atgatcaacc gaggcaccgg cagcatgcct1140agcgggactc accttccagt tcagattgaa agagtgaccg atacatccat ccgcattttt1200gtgccggatc acggctattt ggggctaggc gaaagccttg ccagcaactg gaatgaaccg1260ttggcgctga atctgggctt ggatgaagcg ttgaccttta ccttgagaaa gaaggagacg1320ggaaatgaca ccatttccat aatcgacatg ctgccgccgg tagcgaacac gactccgtct1380ccgccgacga gggaaacgct ttccttgacg ccaaacagct tccgtctgct ggtcaaccct1440gagccgacag cggaggacat cgccaagcac tacaacgtca cgacggtaac ccgggctcct1500gccgatctgg cctccgcctt aaatgttgtc gatgatttct gcttgaaaac cggtttgagc1560tttaacgaat tgctggattt aaccatgcag aaggattatc agtcaaaaag cagtgagtac1620aaaagccgat ttgtaaaatt cggcggcggg gagaatgttc cggtatcaag ctatggcgca1680
gcctttctga caggagcgga agatactcct ttgtgggtga aacagtataa cagcgtgggg1740actgcaacaa gcacccctgt tttaaacttt acgccagata atgttgtggc tttggcagga1800agggcggaaa agcttgtccg gctgatgcgc agcacgggtc tttcctttga gcagttggat1860tggctgattg ccaatgccag ccgtgccgtt atcgaacacg gtggagagct ttttctggat1920aagccggtac tggaagctgt ggccgaattc acaaggctca ataagcgtta tggcgtcaca1980tcggatatgt tcgccgcgtt tatcggcgaa gtcaatacgt atacagaagc gggcaaggac2040agcttttatc aggcgagttt cagcacggcc gaccattcgg ctaccttacc tttgggcgct2100tctttgcaac ttgaggtgag caagcaggat cgatatgaag cgatttgctg cggggctatg2160ggggtgaccg ccgatgagtt ctcccgtatc ggcaaatact gctttgggga taaagcacag2220caaatcacgg ccaatgaaac aaccgttgcc cagctttatc gtttaggccg aattcctcat2280atgctaggct tgcgttttac cgaggcagag ctgttgtgga aattgatggc tgggggcgag2340gataccttgc tccgcacgat tggcgcgaac cctcgcagtt tagaagcgtt agagattatt2400cgccggacgg aggtcctttt ggactggatg gatgcccatc agctggatgt tgtctccctg2460caagccatgg ttaccaatcg gtacagcggc acagccacgc cggagctgta caattttttg2520gcacaggtgc atcaatccgc aagcagtgcc gcgaacgtgg ccagagcgga tggtcaggat2580acgttgcctg cggacaagct gctccgggca ttggcggcgg gcttcaaact gaaagccaac2640gtgatggcgc gagtaatcga ctggatggac aaaaccaata aagcgtttac gctgcgggct2700ttctgggaca agcttcaagc gtatttcagc gccgatcatg aagaagaact gaccgccctg2760gaaggagaag ccgcaatgct gcagtggtgc cagcagatca gccagtatgc gctcattgtc2820cgctggtgcg ggttaagcga gcaggatctg gcgctgctga ccgggaatcc ggagcagctt2880ctggacggac aacatacggt gcccgtaccc tcgctgcatc tcctgctggt gctgacccgc2940ctgaaggaat ggcagcagcg cgtccaggtt tccagcgagg aggctatgcg ctattttgcc3000
caggccgatt cgccaaccgt cacgcgcgac gatgcggtta atctgcttgc ccgtatccat 3060ggctggaatg aagcggatac cgtctcgatg aatgactacc tgctgggaga gaacgaatat 3120cctaagaact ttgatcagat ctttgcactg gaaagctggg tcaacctggg ccgtcaactg 3180aacgtgggca gcagaacgct gggagagctg gttgacatgg ctgaagagga taaaaccgcg 3240gaaaacatgg atctgattac ttcggtggcc catagcctga tggctgcagc gaaagcctga 3300<210>36<211>1080<212>PRT<213>类芽孢杆菌DAS1529株<400>36Met Val Ser Thr Thr Asp Asn Thr Ala Gly Val Phe Arg Leu Gly Thr1 5 10 15Glu Glu Leu Thr Glu Ala Leu Lys Gln Ser Gly Tyr Arg Thr Val Phe20 25 30Asp Ile Val Ser Asp Asn Leu Ala Glu Phe Gln Lys Asn Asn Pro Glu35 40 45Ile Pro Ser Ser Asp Ala Lys Glu Ile His Gln Leu Ala Val Gln Arg50 55 60Thr Glu Asn Leu Cys Met Leu Tyr Lys Ala Trp Gln Leu His Asn Asp65 70 75 80Pro Val Val Gln Ser Leu Pro Lys Leu Ser Ala Asp Thr Gly Leu Gln85 90 95Gly Met Arg Ala Ala Leu Glu Arg Ser Leu Gly Gly Gly Ala Asp Phe
100 105 110Gly Asp Leu Phe Pro Glu Arg Ser Pro Glu Gly Tyr Ala Glu Ala Ser115 120 125Ser Ile Gln Ser Leu Phe Ser Pro Gly Arg Tyr Leu Thr Val Leu Tyr130 135 140Lys Ile Ala Arg Asp Leu His Asp Pro Lys Asp Lys Leu His Ile Asp145 150 155 160Asn Arg Arg Pro Asp Leu Lys Ser Leu Ile Leu Asn Asn Asp Asn Met165 170 175Asn Arg Glu Val Ser Ser Leu Asp Ile Leu Leu Asp Val Leu Gln Pro180 185 l90Glu Gly Ser Asp Thr Leu Thr Ser Leu Lys Asp Thr Tyr His Pro Met195 200 205Thr Leu Pro Tyr Asp Asp Asp Leu Ala Gln Ile Asn Ala Val Ala Glu210 215 220Ala Arg Ser Ser Asn Leu Leu Gly Ile Trp Asp Thr Leu Leu Asp Thr225 230 235 240Gln Arg Thr Ser Ile Leu Gln Asn Ser Ala Ala Ala Arg Arg Ile Ser245 250 255Lys Ala Arg His Ser Ala Tyr Ala Asn Gln Lys Ala Ser Asn Asp Glu260 265 270Pro Val Phe Ile Thr Gly Glu Glu Ile Tyr Leu Glu Thr Gly Gly Lys
275 280 285Arg Leu Phe Leu Ala His Lys Leu GluIle Gly Ser Thr Ile Ser Ala290 295 300Lys Ile Asn Ile Gly Pro Pro Gln Ala Ala Asp Ile Ala Pro Ala Lys305 310 315 320Leu Gln Leu Val Tyr Tyr Gly Arg Gly Gly Arg Gly Asn Tyr Phe Leu325 330 335Arg Val Ala Asp Asp Val Ser Leu Gly Gly Lys Leu Leu Thr Asn Cys340 345 350Tyr Leu Thr Ser Asp Asp Gly Gln Ser Asn Asn Ile Ser Gly Pro Tyr355 360 365Cys Leu Met Ile Asn Arg Gly Thr Gly Ser Met Pro Ser Gly Thr His370 375 380Leu Pro Val Gln Ile Glu Arg Val Thr Asp Thr Ser Ile Arg Ile Phe385 390 395 400Val Pro Asp His Gly Tyr Leu Gly Leu Gly Glu Ser Leu Ala Ser Asn405 410 415Trp Asn Glu Pro Leu Ala Leu Asn Leu Gly Leu Asp Glu Ala Leu Thr420 425 430Phe Thr Leu Arg Lys Lys Glu Thr Gly Asn Asp Thr Ile Ser Ile Ile435 440 445Asp Met Leu Pro Pro Val Ala Asn Thr Thr Pro Ser Pro Pro Thr Arg
450 455 460Glu Thr Leu Ser Leu Thr Pro Asn Ser Phe Arg Leu Leu Val Asn Pro465 470 475 480Glu Pro Thr Ala Glu Asp Ile Ala Lys His Tyr Asn Val Thr Thr Val485 490 495Thr Arg Ala Pro Ala Asp Leu Ala Ser Ala Leu Asn Val Val Asp Asp500 505 510Phe Cys Leu Lys Thr Gly Leu Ser Phe Asn Glu Leu Leu Asp Leu Thr515 520 525Met Gln Lys Asp Tyr Gln Ser Lys Ser Ser Glu Tyr Lys Ser Arg Phe530 535 540Val Lys Phe Gly Gly Gly Glu Asn Val Pro Val Ser Ser Tyr Gly Ala545 550 555 560Ala Phe Leu Thr Gly Ala Glu Asp Thr Pro Leu Trp Val Lys Gln Tyr565 570 575Asn Ser Val Gly Thr Ala Thr Ser Thr Pro Val Leu Asn Phe Thr Pro580 585 590Asp Asn Val Val Ala Leu Ala Gly Arg Ala Glu Lys Leu Val Arg Leu595 600 605Met Arg Ser Thr Gly Leu Ser Phe Glu Gln Leu Asp Trp Leu Ile Ala610 615 620Asn Ala Ser Arg Ala Val Ile Glu His Gly Gly Glu Leu Phe Leu Asp
625 630 635 640Lys Pro Val Leu Glu Ala Val Ala Glu Phe Thr Arg Leu Asn Lys Arg645 650 655Tyr Gly Val Thr Ser Asp Met Phe Ala Ala Phe Ile Gly Glu Val Asn660 665 670Thr Tyr Thr Glu Ala Gly Lys Asp Ser Phe Tyr Gln Ala Ser Phe Ser675 680 685Thr Ala Asp His Ser Ala Thr Leu Pro Leu Gly Ala Ser Leu Gln Leu690 695 700Glu Val Ser Lys Gln Asp Arg Tyr Glu Ala Ile Cys Cys Gly Ala Met705 710 715 720Gly Val Thr Ala Asp Glu Phe Ser Arg Ile Gly Lys Tyr Cys Phe Gly725 730 735Asp Lys Ala Gln Gln Ile Thr Ala Asn Glu Thr Thr Val Ala Gln Leu740 745 750Tyr Arg Leu Gly Arg Ile Pro His Met Leu Gly Leu Arg Phe Thr Glu755 760 765Ala Glu Leu Leu Trp Lys Leu Met Ala Gly Gly Glu Asp Thr Leu Leu770 775 780Arg Thr Ile Gly Ala Asn Pro Arg Ser Leu Glu Ala Leu Glu Ile Ile785 790 795 800Arg Arg Thr Glu Val Leu Leu Asp Trp Met Asp Ala His Gln Leu Asp
805 810 815Val Val Ser Leu Gln Ala Met Val Thr Asn Arg Tyr Ser Gly Thr Ala820 825 830Thr Pro Glu Leu Tyr Asn Phe Leu Ala Gln Val His Gln Ser Ala Ser835 840 845Ser Ala Ala Asn Val Ala Arg Ala Asp Gly Gln Asp Thr Leu Pro Ala850 855 860Asp Lys Leu Leu Arg Ala Leu Ala Ala Gly Phe Lys Leu Lys Ala Asn865 870 875 880Val Met Ala Arg Val Ile Asp Trp Met Asp Lys Thr Asn Lys Ala Phe885 890 895Thr Leu Arg Ala Phe Trp Asp Lys Leu Gln Ala Tyr Phe Ser Ala Asp900 905 910His Glu Glu Glu Leu Thr Ala Leu Glu Gly Glu Ala Ala Met Leu Gln915 920 925Trp Cys Gln Gln Ile Ser Gln Tyr Ala Leu Ile Val Arg Trp Cys Gly930 935 940Leu Ser Glu Gln Asp Leu Ala Leu Leu Thr Gly Asn Pro Glu Gln Leu945 950 955 960Leu Asp Gly Gln His Thr Val Pro Val Pro Ser Leu His Leu Leu Leu965 970 975Val Leu Thr Arg Leu Lys Glu Trp Gln Gln Arg Val Gln Val Ser Ser
980 985 990Glu Glu Ala Met Arg Tyr Phe Ala Gln Ala Asp Ser Pro Thr Val Thr995 10001005Arg Asp Asp Ala Val Asn Leu Leu Ala Arg Ile His Gly Trp Asn1010 1015 1020Glu Ala Asp Thr Val Ser Met Asn Asp Tyr Leu Leu Gly Glu Asn1025 1030 1035Glu Tyr Pro Lys Ash Phe Asp Gln Ile Phe Ala Leu Glu Ser Trp1040 1045 1050Val Asn Leu Gly Arg Gln Leu Asn Val Gly Ser Arg Thr Leu Gly1055 1060 1065Glu Leu Val Asp Met Ala Glu Glu Asp Lys Thr Ala1070 1075 1080<210>37<211>3627<212>DNA<213>类芽孢杆菌DAS1529株<400>37atgaccaagg aaggtgataa gcatatgtct acttcaaccc tgttgcaatc gattaaagaa 60gcccgccggg atgcgctggt caaccattat attgctaatc aggttccgac agcgcttgcg 120gacaagatta cggacgcgga cagcctgtat gagtacttgc tgctggatac caagatcagt 180gaactcgtaa aaacatcgcc gatagcggag gccatcagca gcgtgcagtt atacatgaac 240cgctgcgtcg aaggctatga aggcaagttg actccggaaa gtaatactca ttttggccca 300ggtaaatttc tatataactg ggatacgtac aacaaacgtt tttccacctg ggcaggaaaa 360
gaacgcttga aatattatgc aggcagctat attgagccgt ccttgcgcta caacaaaacc 420gatccattcc tgaacctgga acagagcatc agccagggaa gaattactga tgataccgta 480aagaacgcgc tgcaacacta cctgactgaa tatgaagtgt tggcggatct ggattatatc 540agcgttaata aaggcggcga cgaaagtgtt ttactctttg ttggacgcac caaaaccgta 600ccgtatgaat actactggcg ccgtttgctt ttaaaaaggg acaataataa taagctagta 660ccagcagtct ggtctcagtg gaaaaaaatc agtgccaata tcggtgaagc ggttgatagt 720tatgtggtgc ctcggtggca taaaaaccgg ctacatgtgc aatggtgttc tatagagaaa 780agtgaaaatg atgccggtga acccattgag aaacgatatt tgaatgactg gttcatggat 840agttccggag tctggtcttc atttcgaaag attccggttg tggaaaagag tttcgaatat 900ttggacggaa gcctcgatcc ccgatttgtc gctcttgtta gaaatcaaat attaattgat 960gagccagaaa tattcagaat tacagtatca gcccctaatc cgatagatgc aaatggaaga1020gtagaggtac attttgaaga aaactatgca aacagatata atattaccat taaatatggg1080acaacgagtc ttgctattcc tgcagggcag gtagggcatc caaatatctc tattaatgaa1140acattaaggg ttgaattcgg caccaggccg gattggtatt atactttcag atatttagga1200aatacaatcc aaaactcata cggttcaatt gtcaataatc aattttcacc tccatcagga1260agcaatatta aaggtcctat cgaccttacc ctgaaaaata acatcgacct gtcggccttg1320ttggatgaga gccttgacgc actgttcgac tataccattc agggcgataa ccaattgggc1380ggcttagctg cctttaacgg gccttacgga ctttacttgt gggaaatctt cttccatgtt1440ccttttttaa tggcggttcg cttccacacc gagcagcggt atgagttggc ggaacgttgg1500tttaaattca tcttcaacag cgcaggatac cgtgatgatt acggcagtct gctgacggat1560gacaaaggca acgtgcgtta ctggaacgtg ataccgctgc aagaggacac ggagtgggat1620gacacgttgt ccctggcaac gaccgacccg gacgagattg cgatggccga cccgatgcaa1680
tacaagctgg ctatatttat tcacaccatg gacttcctga tcagccgcgg cgatagcttg1740taccggatgc tggagcggga taccctggcc gaagccaaga tgtattacat tcaggccagc1800caactgcttg ggccccgccc cgacatccgg ctcaatcaca gttggcctaa tccgaccttg1860caaagcgaag cggacgcggt aaccgccgtg ccgacgcgaa gcgattcgcc ggcagcgcca1920attttggcct tgcgagcgct tctgacaggc gaaaacggtc atttcctgcc gccttataat1980gatgaactgt tcgctttctg ggacaaaatc gatctgcgtt tatacaattt gcgccacaat2040ttgagtctgg acggtcagcc gcttcatttg ccgctctttg ccgaaccggt caatccgcgt2100gaattgcagg ttcagcatgg cccgggcgat ggcttggggg gaagcgcggg ttccgcccaa2160agccgtcaga gtgtctatcg ttttcctctg gtcatcgata aggcgcgcaa tgcggccaac2220agtgtcatcc aattcggcaa tgccctggaa aacgcactga ccaagcaaga cagcgaagca2280atgaccatgc tgttgcagtc ccagcagcag attgtcctgc agcaaacccg cgatattcag2340gagaagaacc tggccgcgct gcaagcaagt ctggaagcaa cgatgacagc gaaagcgggg2400gcggagtccc ggaagaccca ttttgccggc ttggcggaca actggatgtc ggacaatgaa2460accgcctcac tcgcactgcg taccaccgcg ggaatcatca ataccagctc aaccgtgccg2520atcgccatca ccggcggctt ggatatggct ccgaacattt ttggtttcgc agttggaggt2580tcccgctggg gagcagccag cgcggctgta gcccaaggat tgcaaatcgc cgccggcgta2640atggaacaga cggccaatat tatcgatatt agcgaaagct accgccggcg ccgggaggat2700tggctgctgc agcgggatgt tgccgaaaat gaagcggcgc agttggattc gcagattgcg2760gccctgcggg aacagatgga tatggcgcgc aagcaacttg cgctggcgga gacggaacag2820gcgcacgcgc aagcggtcta cgagctgcaa agcacccgct ttacgaatca agctttgtat2880aactggatgg ctggacgtct gtcgtctcta tactatcaaa tgtatgacgc cgcattgccg2940ctctgcttga tggcgaagca ggctttagag aaagaaatcg gttcggataa aacggtcgga3000
gtcttgtccc tcccggcctg gaatgatcta tatcagggat tattggcggg cgaggcgctg 3060ctgctcgagc ttcagaagct ggagaatctg tggctggagg aagacaagcg cggaatggaa 3120gccgtaaaaa cagtctctct ggatactctt ctccgcaaaa caaatccgaa ctccgggttt 3180gcggatctcg tcaaggaggc actggacgaa aacggaaaga cgcctgaccc ggtgagcgga 3240gtcggcgtac agctgcaaaa caatattttc agcgcaaccc ttgacctctc cgttcttggc 3300ctggatcgct cttacaatca ggcggaaaag tcccgcagga tcaaaaatat gtcggttacc 3360ttacctgcgc tattggggcc ttaccaggat atagaggcaa ccttatcgct aggcggcgag 3420accgttgcgc tgtcccatgg cgtggatgac agcggcttgt tcatcactga tctcaacgac 3480agccggttcc tgcctttcga gggcatggat ccgttatccg gcacactcgt cctgtcgata 3540ttccatgccg ggcaagacgg cgaccagcgc ctcctgctgg aaagtctcaa tgacgtcatc 3600ttccacattc gatatgttat gaaatag 3627<210>38<211>1208<212>PRT<213>类芽孢杆菌DAS1529株<400>38Met Thr Lys Glu Gly Asp Lys His Met Ser Thr Ser Thr Leu Leu Gln1 5 10 15Ser Ile Lys Glu Ala Arg Arg Asp Ala Leu Val Asn His Tyr Ile Ala20 25 30Asn Gln Val Pro Thr Ala Leu Ala Asp Lys Ile Thr Asp Ala Asp Ser35 40 45Leu Tyr Glu Tyr Leu Leu Leu Asp Thr Lys Ile Ser Glu Leu Val Lys
50 55 60Thr Ser Pro Ile Ala Glu Ala Ile Ser Ser Val Gln Leu Tyr Met Asn65 70 75 80Arg Cys Val Glu Gly Tyr Glu Gly Lys Leu Thr Pro Glu Ser Asn Thr85 90 95His Phe Gly Pro Gly Lys Phe Leu Tyr Asn Trp Asp Thr Tyr Asn Lys100 105 110Arg Phe Ser Thr Trp Ala Gly Lys Glu Arg Leu Lys Tyr Tyr Ala Gly115 120 125Ser Tyr Ile Glu Pro Ser Leu Arg Tyr Asn Lys Thr Asp Pro Phe Leu130 135 140Asn Leu Glu Gln Ser Ile Ser Gln Gly Arg Ile Thr Asp Asp Thr Val145 150 155 160Lys Asn Ala Leu Gln His Tyr Leu Thr Glu Tyr Glu Val Leu Ala Asp165 170 175Leu Asp Tyr Ile Ser Val Asn Lys Gly Gly Asp Glu Ser Val Leu Leu180 185 190Phe Val Gly Arg Thr Lys Thr Val Pro Tyr Glu Tyr Tyr Trp Arg Arg195 200 205Leu Leu Leu Lys Arg Asp Asn Asn Asn Lys Leu Val Pro Ala Val Trp210 215 220Ser Gln Trp Lys Lys Ile Ser Ala Asn Ile Gly Glu Ala Val Asp Ser
225 230 235 240Tyr Val Val Pro Arg Trp His Lys Asn Arg Leu His Val Gln Trp Cys245 250 255Ser Ile Glu Lys Ser Glu Asn Asp Ala Gly Glu Pro Ile Glu Lys Arg260 265 270Tyr Leu Asn Asp Trp Phe Met Asp Ser Ser Gly Val Trp Ser Ser Phe275 280 285Arg Lys Ile Pro Val Val Glu Lys Ser Phe Glu Tyr Leu Asp Gly Ser290 295 300Leu Asp Pro Arg Phe Val Ala Leu Val Arg Asn Gln Ile Leu Ile Asp305 310 315 320Glu Pro Glu Ile Phe Arg Ile Thr Val Ser Ala Pro Asn Pro Ile Asp325 330 335Ala Asn Gly Arg Val Glu Val His Phe Glu Glu Asn Tyr Ala Asn Arg340 345 350Tyr Asn Ile Thr Ile Lys Tyr Gly Thr Thr Ser Leu Ala Ile Pro Ala355 360 365Gly Gln Val Gly His Pro Asn Ile Ser Ile Asn Glu Thr Leu Arg Val370 375 380Glu Phe Gly Thr Arg Pro Asp Trp Tyr Tyr Thr Phe Arg Tyr Leu Gly385 390 395 400Asn Thr Ile Gln Asn Ser Tyr Gly Ser Ile Val Asn Asn Gln Phe Ser
405 410 415Pro Pro Ser Gly Ser Asn Ile Lys Gly Pro Ile Asp Leu Thr Leu Lys420 425 430Asn Asn Ile Asp Leu Ser Ala Leu Leu Asp Glu Ser Leu Asp Ala Leu435 440 445Phe Asp Tyr Thr Ile Gln Gly Asp Asn Gln Leu Gly Gly Leu Ala Ala450 455 460Phe Asn Gly Pro Tyr Gly Leu Tyr Leu Trp Glu Ile Phe Phe His Val465 470 475 480Pro Phe Leu Met Ala Val Arg Phe His Thr Glu Gln Arg Tyr Glu Leu485 490 495Ala Glu Arg Trp Phe Lys Phe Ile Phe Asn Ser Ala Gly Tyr Arg Asp500 505 510Asp Tyr Gly Ser Leu Leu Thr Asp Asp Lys Gly Asn Val Arg Tyr Trp515 520 525Asn Val Ile Pro Leu Gln Glu Asp Thr Glu Trp Asp Asp Thr Leu Ser530 535 540Leu Ala Thr Thr Asp Pro Asp Glu Ile Ala Met Ala Asp Pro Met Gln545 550 555 560Tyr Lys Leu Ala Ile Phe Ile His Thr Met Asp Phe Leu Ile Ser Arg565 570 575Gly Asp Ser Leu Tyr Arg Met Leu Glu Arg Asp Thr Leu Ala Glu Ala
580 585 590Lys Met Tyr Tyr Ile Gln Ala Ser Gln Leu Leu Gly Pro Arg Pro Asp595 600 605Ile Arg Leu Asn His Ser Trp Pro Asn Pro Thr Leu Gln Ser Glu Ala610 615 620Asp Ala Val Thr Ala Val Pro Thr Arg Ser Asp Ser Pro Ala Ala Pro625 630 635 640Ile Leu Ala Leu Arg Ala Leu Leu Thr Gly Glu Asn Gly His Phe Leu645 650 655Pro Pro Tyr Asn Asp Glu Leu Phe Ala Phe Trp Asp Lys Ile Asp Leu660 665 670Arg Leu Tyr Asn Leu Arg His Asn Leu Ser Leu Asp Gly Gln Pro Leu675 680 685His Leu Pro Leu Phe Ala Glu Pro Val Asn Pro Arg Glu Leu Gln Val690 695 700Gln His Gly Pro Gly Asp Gly Leu Gly Gly Ser Ala Gly Ser Ala Gln705 710 715 720Ser Arg Gln Ser Val Tyr Arg Phe Pro Leu Val Ile Asp Lys Ala Arg725 730 735Asn Ala Ala Asn Ser Val Ile Gln Phe Gly Asn Ala Leu Glu Asn Ala740 745 750Leu Thr Lys Gln Asp Ser Glu Ala Met Thr Met Leu Leu Gln Ser Gln
755 760 765Gln Gln Ile Val Leu Gln Gln Thr Arg Asp Ile Gln Glu Lys Asn Leu770 775 780Ala Ala Leu Gln Ala Ser Leu Glu Ala Thr Met Thr Ala Lys Ala Gly785 790 795 800Ala Glu Ser Arg Lys Thr His Phe Ala Gly Leu Ala Asp Asn Trp Met805 810 8l5Ser Asp Asn Glu Thr Ala Ser Leu Ala Leu Arg Thr Thr Ala Gly Ile820 825 830Ile Asn Thr Ser Ser Thr Val Pro Ile Ala Ile Thr Gly Gly Leu Asp835 840 845Met Ala Pro Asn Ile Phe Gly Phe Ala Val Gly Gly Ser Arg Trp Gly850 855 860Ala Ala Ser Ala Ala Val Ala Gln Gly Leu Gln Ile Ala Ala Gly Val865 870 875 880Met Glu Gln Thr Ala Asn Ile Ile Asp Ile Ser Glu Ser Tyr Arg Arg885 890 895Arg Arg Glu Asp Trp Leu Leu Gln Arg Asp Val Ala Glu Asn Glu Ala900 905 910Ala Gln Leu Asp Ser Gln Ile Ala Ala Leu Arg Glu Gln Met Asp Met915 920 925Ala Arg Lys Gln Leu Ala Leu Ala Glu Thr Glu Gln Ala His Ala Gln
930 935 940Ala Val Tyr Glu Leu Gln Ser Thr Arg Phe Thr Asn Gln Ala Leu Tyr945 950 955 960Asn Trp Met Ala Gly Arg Leu Ser Ser Leu Tyr Tyr Gln Met Tyr Asp965 970 975Ala Ala Leu Pro Leu Cys Leu Met Ala Lys Gln Ala Leu Glu Lys Glu980 985 990Ile Gly Ser Asp Lys Thr Val Gly Val Leu Ser Leu Pro Ala Trp Asn995 10001005Asp Leu Tyr Gln Gly Leu Leu Ala Gly Glu Ala Leu Leu Leu Glu1010 1015 1020Leu Gln Lys Leu Glu Asn Leu Trp Leu Glu Glu Asp Lys Arg Gly1025 1030 1035Met Glu Ala Val Lys Thr Val Ser Leu Asp Thr Leu Leu Arg Lys1040 1045 1050Thr Asn Pro Asn Ser Gly Phe Ala Asp Leu Val Lys Glu Ala Leu1055 1060 1065Asp Glu Asn Gly Lys Thr Pro Asp Pro Val Ser Gly Val Gly Val1070 1075 1080Gln Leu Gln Asn Asn Ile Phe Ser Ala Thr Leu Asp Leu Ser Val1085 1090 1095Leu Gly Leu Asp Arg Ser Tyr Asn Gln Ala Glu Lys Ser Arg Arg
1100 1105 1110Ile Lys Asn Met Ser Val Thr Leu Pro Ala Leu Leu Gly Pro Tyr1115 1120 1125Gln Asp Ile Glu Ala Thr Leu Ser Leu Gly Gly Glu Thr Val Ala1130 1135 1140Leu Ser His Gly Val Asp Asp Ser Gly Leu Phe Ile Thr Asp Leu1145 1150 1155Asn Asp Ser Arg Phe Leu Pro Phe Glu Gly Met Asp Pro Leu Set1160 1165 1170Gly Thr Leu Val Leu Ser Ile Phe His Ala Gly Gln Asp Gly Asp1175 1180 1185Gln Arg Leu Leu Leu Glu Ser Leu Asn Asp Val Ile Phe His Ile1190 1195 1200Arg Tyr Val Met Lys1205<210>39<211>4335<212>DNA<213>类芽孢杆菌DAS1529株<400>39atgccacaat ctagcaatgc cgatatcaag ctattgtcgc catcgctgcc aaagggcggc 60ggttccatga agggaatcga agaaaacatc gcggctcccg gctccgacgg catggcacgt120tgtaatgtgc cgctgccggt aacctccggc cgctatatta ctcctgatat aagcctgtcc180tatgcgagcg gccacggcaa cggcgcttat ggaatgggct ggacgatggg agtgatgagc240
attagccgga gaacaagccg agggaccccc agttatacat ccgaagacca gttccttggt 300ccggatgggg aggtgcttgt tccggaaagc aacgaacaag gggagatcat tacccgccac 360accgatacgg cccaagggat accgttaggc gagacgttta cggttacacg ctattttccc 420cggatcgaga gcgcttttca tttgctggaa tactgggaag cgcaagcagg aagcgcaaca 480gcgtcgtttt ggcttattca ctctgccgat ggagtgctgc actgtctggg taaaactgct 540caggcgagga tagccgcccc tgacgattcc gccaagatcg cagaatggct agtggaggag 600tccgtctccc ccttcggaga gcatatttat taccaataca aagaagaaga caatcaaggc 660gtgaatctgg aggaagacaa tcatcaatat ggggcgaacc gctatctgaa atcgattcgc 720tatggaaata aggttgcctc tccttctctc tatgtctgga agggggaaat tccggcagac 780ggccaatggc tgtattccgt tatcctggat tatggcgaga acgatacctc agcggatgtt 840cctcccctat acacgcccca aggggagtgg ctggtgcgcc cggaccgttt ttcccgctat 900gactacggat ttgaggtccg gacttgccgc ttgtgccgcc aggtcttgat gttccacgtc 960tttaaggagc ttggcgggga gccggcgctg gtgtggcgga tgcagttgga atacgacgag1020aacccggcgg cgtccatgct gagcgcggtc cggcaattgg cttatgaagc agatggggcc1080attcgaagct tgccgccgct ggaattcgat tatactccat ttggcatcga gacaacggcc1140gattggcagc cttttctgcc tgtgcctgaa tgggcggatg aagaacatta tcagttggtc1200gatttgtacg gagaaggcat accgggctta ttatatcaga acaatgacca ctggcattat1260cgttcgcccg cccggggcga cacaccggac gggatcgcct ataacagctg gcggccgctt1320cctcatatcc ccgtgaactc ccggaacggg atgctgatgg atctgaatgg agacgggtat1380ctggaatggt tgcttgcgga acccggggtt gcggggcgct atagcatgaa cccggataag1440agctggtccg gttttgtgcc gctccaggca ctgccaacgg aattcttcca tccgcaggca1500cagcttgcca atgttaccgg atcgggttta accgacttgg ttatgatcgg tccgaagagc1560
gtccggtttt atgccggaga agaagcgggc ttcaagcgcg catgtgaagt gtggcagcaa1620gtgggcatta ctttgcctgt ggaacgcgtg gataaaaagg aactggtggc attcagcgat1680atgctgggat cgggtcagtc tcatctggtg cgcatccggc atgatggcgt tacatgctgg1740cctaatctgg ggaacggcgt gttcggggcg ccgttggccc ttcacgggtt tacggcatcg1800gagcgggaat tcaatccgga acgtgtatat cttgtggacc ttgatggatc cggcgcttcc1860gatatcattt atgcttctcg tgacgctcta ctcatttacc gaaatctttc cggcaatggc1920tttgctgatc cggtgcgggt tccgctgcct gacggcgtgc ggtttgataa tctgtgccgg1980ctgctgcctg ccgatatccg cgggttaggt gtggccagtc tggtgctgca tgtaccttac2040atggcccccc gcagttggaa attagatttc tttgcggcga agccgtattt attgcaaacg2100gtcagcaaca atcttggagc ttccagctcg ttttggtacc gaagctccac ccagtattgg2160ctggatgaga aacaggcggc ctcatcggct gtctccgctt tgcccttccc gataaacgtg2220gtatcggata tgcacacggt ggacgaaatc agcggccgca ccaggactca gaagtatact2280taccgccatg gcgtgtatga ccggaccgaa aaggaatttg ccggattcgg ccgcattgac2340acatgggaag aggagcggga ttccgaagga accctgagcg tcagcactcc gcccgtgctg2400acgcggacct ggtatcatac cgggcaaaag caggatgagg agcgtgccgt gcagcaatat2460tggcaaggcg accctgcggc ttttcaggtt aaacccgtcc ggcttactcg attcgatgcg2520gcagcggccc aggatctgcc gctagattct aataatgggc agcaagaata ctggctgtac2580cgatcattac aagggatgcc gctgcggact gagatttttg cgggagatgt tggcgggtcg2640cctccttatc aggtagagag cttccgttat caagtgcgct tggtgcagag catcgattcg2700gaatgtgttg ccttgcccat gcagttggag cagcttacgt acaactatga gcaaatcgcc2760tctgatccgc agtgttcaca gcagatacag caatggttcg acgaatacgg cgtggcggca2820cagagtgtaa caatccaata tccgcgccgg gcacagccgg aggacaatcc gtaccctcgc2880
acgctgccgg ataccagctg gagcagcagt tatgattcgc agcaaatgct gctgcggttg2940accaggcaaa ggcaaaaagc gtaccacctt gcagatcctg aaggctggcg cttgaatatt3000ccccatcaga cacgcctgga tgccttcatt tattctgctg acagcgtgcc cgccgaagga3060ataagcgccg agctgctgga ggtggacggc acgttacgat cttcggcgct ggaacaggct3120tatggcggcc agtcagagat catctatgcg ggcgggggcg aaccggattt gcgagccctg3180gtccattaca ccagaagcgc ggttcttgat gaagactgtt tacaagccta tgaaggcgta3240ctgagcgata gccaattgaa ctcgcttctt gcctcttccg gctatcaacg aagcgcaaga3300atattgggtt cgggcgatga agtggatatt tttgtcgcgg aacaaggatt tacccgttat3360gcggatgaac cgaatttttt ccgtattctg gggcaacaat cctctctctt gtccggggaa3420caagtattaa catgggatga taatttctgt gcggttacat ccatcgaaga cgcgcttggc3480aatcaaattc agattgcata tgattaccgc tttgtggagg ccatccagat taccgatacg3540aataataatg tgaatcaggt cgccctggat gctctcggcc gggtcgtata cagccggacc3600tggggcacgg aggaagggat aaagaccggc ttccgcccgg aggtggaatt cgcgacgccc3660gagacaatgg agcaggcgct tgccctggca tctcccttgc cggttgcatc ctgctgtgta3720tatgatgcgc atagctggat gggaacgata actcttgcac aactgtcaga gcttgttcca3780gatagtgaaa agcaatggtc gttcttgata gacaatcgct tgattatgcc ggacggcaga3840atcagatccc gcggtcggga tccatggtcg cttcaccggc tattgccgcc tgctgtgggc3900gaattgctga gcgaggcgga ccgtaaaccg ccgcatacgg taattttggc agcagatcgt3960tacccggatg acccatccca gcaaattcag gcgagcatcg tgtttagcga tggctttggg4020cgtacgatac aaactgctaa aagagaagat acccgatggg cgattgcgga acgggtggac4080tatgacggaa ccggagccgt aatccgcagc tttcagcctt tttatcttga cgactggaat4140tatgtgggcg aagaggctgt cagcagctct atgtacgcaa cgatctatta ttatgatgct4200
ctggcacgac aattaaggat ggtcaacgct aaaggatatg agaggagaac tgctttttac 4260ccatggttta cagtaaacga agatgaaaat gataccatgg actcatcatt atttgcttca 4320ccgcctgcgc ggtga4335<210>40<211>1444<212>PRT<213>类芽孢杆菌DAS1529株<400>40Met Pro Gln Ser Ser Asn Ala Asp Ile Lys Leu Leu Ser Pro Ser Leu1 5 10 15Pro Lys Gly Gly Gly Ser Met Lys Gly Ile Glu Glu Asn Ile Ala Ala20 25 30Pro Gly Ser Asp Gly Met Ala Arg Cys Asn Val Pro Leu Pro Val Thr35 40 45Ser Gly Arg Tyr Ile Thr Pro Asp Ile Ser Leu Ser Tyr Ala Ser Gly50 55 60His Gly Asn Gly Ala Tyr Gly Met Gly Trp Thr Met Gly Val Met Ser65 70 75 80Ile Ser Arg Arg Thr Ser Arg Gly Thr Pro Ser Tyr Thr Ser Glu Asp85 90 95Gln Phe Leu Gly Pro Asp Gly Glu Val Leu Val Pro Glu Ser Asn Glu100 105 110Gln Gly Glu Ile Ile Thr Arg His Thr Asp Thr Ala Gln Gly Ile Pro
115 120 125Leu Gly Glu Thr Phe Thr Val Thr Arg Tyr Phe Pro Arg Ile Glu Ser130 135 140Ala Phe His Leu Leu Glu Tyr Trp Glu Ala Gln Ala Gly Ser Ala Thr145 150 155 160Ala Ser Phe Trp Leu Ile His Ser Ala Asp Gly Val Leu His Cys Leu165 170 175Gly Lys Thr Ala Gln Ala Arg Ile Ala Ala Pro Asp Asp Ser Ala Lys180 185 190Ile Ala Glu Trp Leu Val Glu Glu Ser Val Ser Pro Phe Gly Glu His195 200 205Ile Tyr Tyr Gln Tyr Lys Glu Glu Asp Asn Gln Gly Val Asn Leu Glu210 215 220Glu Asp Asn Hi s Gln Tyr Gly Ala Asn Arg Tyr Leu Lys Ser Ile Arg225 230 235 240Tyr Gly Asn Lys Val Ala Ser Pro Ser Leu Tyr Val Trp Lys Gly Glu245 250 255Ile Pro Ala Asp Gly Gln Trp Leu Tyr Ser Val Ile Leu Asp Tyr Gly260 265 270Glu Asn Asp Thr Ser Ala Asp Val Pro Pro Leu Tyr Thr Pro Gln Gly275 280 285Glu Trp Leu Val Arg Pro Asp Arg Phe Ser Arg Tyr Asp Tyr Gly Phe
290 295 300Glu Val Arg Thr Cys Arg Leu Cys Arg Gln Val Leu Met Phe His Val305 310 315 320Phe Lys Glu Leu Gly Gly Glu Pro Ala Leu Val Trp Arg Met Gln Leu325 330 335Glu Tyr Asp Glu Asn Pro Ala Ala Ser Met Leu Ser Ala Val Arg Gln340 345 350Leu Ala Tyr Glu Ala Asp Gly Ala Ile Arg Ser Leu Pro Pro Leu Glu355 360 365Phe Asp Tyr Thr Pro Phe Gly Ile Glu Thr Thr Ala Asp Trp Gln Pro370 375 380Phe Leu Pro Val Pro Glu Trp Ala Asp Glu Glu His Tyr Gln Leu Val385 390 395 400Asp Leu Tyr Gly Glu Gly Ile Pro Gly Leu Leu Tyr Gln Asn Asn Asp405 410 415His Trp His Tyr Arg Ser Pro Ala Arg Gly Asp Thr Pro Asp Gly Ile420 425 430Ala Tyr Asn Ser Trp Arg Pro Leu Pro His Ile Pro Val Asn Ser Arg435 440 445Asn Gly Met Leu Met Asp Leu Asn Gly Asp Gly Tyr Leu Glu Trp Leu450 455 460Leu Ala Glu Pro Gly Val Ala Gly Arg Tyr Ser Met Asn Pro Asp Lys
465 470 475 480Ser Trp Ser Gly Phe Val Pro Leu Gln Ala Leu Pro Thr Glu Phe Phe485 490 495His Pro Gln Ala Gln Leu Ala Asn Val Thr Gly Ser Gly Leu Thr Asp500 505 510Leu Val Met Ile Gly Pro Lys Ser Val Arg Phe Tyr Ala Gly Glu Glu515 520 525Ala Gly Phe Lys Arg Ala Cys Glu Val Trp Gln Gln Val Gly Ile Thr530 535 540Leu Pro Val Glu Arg Val Asp Lys Lys Glu Leu Val Ala Phe Ser Asp545 550 555 560Met Leu Gly Ser Gly Gln Ser His Leu Val Arg Ile Arg His Asp Gly565 570 575Val Thr Cys Trp Pro Asn Leu Gly Asn Gly Val Phe Gly Ala Pro Leu580 585 590Ala Leu His Gly Phe Thr Ala Ser Glu Arg Glu Phe Asn Pro Glu Arg595 600 605Val Tyr Leu Val Asp Leu Asp Gly Ser Gly Ala Ser Asp Ile Ile Tyr610 615 620Ala Ser Arg Asp Ala Leu Leu Ile Tyr Arg Asn Leu Ser Gly Asn Gly625 630 635 640Phe Ala Asp Pro Val Arg Val Pro Leu Pro Asp Gly Val Arg Phe Asp
645 650 655Asn Leu Cys Arg Leu Leu Pro Ala Asp Ile Arg Gly Leu Gly Val Ala660 665 670Ser Leu Val Leu His Val Pro Tyr Met Ala Pro Arg Ser Trp Lys Leu675 680 685Asp Phe Phe Ala Ala Lys Pro Tyr Leu Leu Gln Thr Val Ser Asn Asn690 695 700Leu Gly Ala Ser Ser Ser Phe Trp Tyr Arg Ser Ser Thr Gln Tyr Trp705 710 715 720Leu Asp Glu Lys Gln Ala Ala Ser Ser Ala Val Ser Ala Leu Pro Phe725 730 735Pro Ile Asn Val Val Ser Asp Met His Thr Val Asp Glu Ile Ser Gly740 745 750Arg Thr Arg Thr Gln Lys Tyr Thr Tyr Arg His Gly Val Tyr Asp Arg755 760 765Thr Glu Lys Glu Phe Ala Gly Phe Gly Arg Ile Asp Thr Trp Glu Glu770 775 780Glu Arg Asp Ser Glu Gly Thr Leu Ser Val Ser Thr Pro Pro Val Leu785 790 795 800Thr Arg Thr Trp Tyr His Thr Gly Gln Lys Gln Asp Glu Glu Arg Ala805 810 815Val Gln Gln Tyr Trp Gln Gly Asp Pro Ala Ala Phe Gln Val Lys Pro
820 825 830Val Arg Leu Thr Arg Phe Asp Ala Ala Ala Ala Gln Asp Leu Pro Leu835 840 845Asp Ser Asn Asn Gly Gln Gln Glu Tyr Trp Leu Tyr Arg Ser Leu Gln850 855 860Gly Met Pro Leu Arg Thr Glu Ile Phe Ala Gly Asp Val Gly Gly Ser865 870 875 880Pro Pro Tyr Gln Val Glu Ser Phe Arg Tyr Gln Val Arg Leu Val Gln885 890 895Ser Ile Asp Ser Glu Cys Val Ala Leu Pro Met Gln Leu Glu Gln Leu900 905 910Thr Tyr Asn Tyr Glu Gln Ile Ala Ser Asp Pro Gln Cys Ser Gln Gln915 920 925Ile Gln Gln Trp Phe Asp Glu Tyr Gly Val Ala Ala Gln Ser Val Thr930 935 940Ile Gln Tyr Pro Arg Arg Ala Gln Pro Glu Asp Asn Pro Tyr Pro Arg945 950 955 960Thr Leu Pro Asp Thr Ser Trp Ser Ser Ser Tyr Asp Ser Gln Gln Met965 970 975Leu Leu Arg Leu Thr Arg Gln Arg Gln Lys Ala Tyr His Leu Ala Asp980 985 990Pro Glu Gly Trp Arg Leu Asn Ile Pro His Gln Thr Arg Leu Asp Ala
995 1000 1005Phe Ile Tyr Ser Ala Asp Ser Val Pro Ala Glu Gly Ile Ser Ala1010 1015 1020Glu Leu Leu Glu Val Asp Gly Thr Leu Arg Ser Ser Ala Leu Glu1025 1030 1035Gln Ala Tyr Gly Gly Gln Ser Glu Ile Ile Tyr Ala Gly Gly Gly1040 1045 1050Glu Pro Asp Leu Arg Ala Leu Val His Tyr Thr Arg Ser Ala Val1055 1060 1065Leu Asp Glu Asp Cys Leu Gln Ala Tyr Glu Gly Val Leu Ser Asp1070 1075 1080Ser Gln Leu Asn Ser Leu Leu Ala Ser Ser Gly Tyr Gln Arg Ser1085 1090 1095Ala Arg Ile Leu Gly Ser Gly Asp Glu Val Asp Ile Phe Val Ala1100 1105 1110Glu Gln Gly Phe Thr Arg Tyr Ala Asp Glu Pro Asn Phe Phe Arg1115 1120 1125Ile Leu Gly Gln Gln Ser Ser Leu Leu Ser Gly Glu Gln Val Leu1130 1135 1140Thr Trp Asp Asp Asn Phe Cys Ala Val Thr Ser Ile Glu Asp Ala1145 1150 1155Leu Gly Asn Gln Ile Gln Ile Ala Tyr Asp Tyr Arg Phe Val Glu
1160 1165 1170Ala Ile Gln Ile Thr Asp Thr Asn Asn Asn Val Asn Gln Val Ala1175 1180 1185Leu Asp Ala Leu Gly Arg Val Val Tyr Ser Arg Thr Trp Gly Thr1190 1195 1200Glu Glu Gly Ile Lys Thr Gly Phe Arg Pro Glu Val Glu Phe Ala1205 1210 1215Thr Pro Glu Thr Met Glu Gln Ala Leu Ala Leu Ala Ser Pro Leu1220 1225 1230Pro Val Ala Ser Cys Cys Val Tyr Asp Ala His Ser Trp Met Gly1235 1240 1245Thr Ile Thr Leu Ala Gln Leu Ser Glu Leu Val Pro Asp Ser Glu1250 1255 1260Lys Gln Trp Ser Phe Leu Ile Asp Asn Arg Leu Ile Met Pro Asp1265 1270 1275Gly Arg Ile Arg Ser Arg Gly Arg Asp Pro Trp Ser Leu His Arg1280 1285 1290Leu Leu Pro Pro Ala Val Gly Glu Leu Leu Ser Glu Ala Asp Arg1295 1300 1305Lys Pro Pro His Thr Val Ile Leu Ala Ala Asp Arg Tyr Pro Asp1310 1315 1320Asp Pro Ser Gln Gln Ile Gln Ala Ser Ile Val Phe Ser Asp Gly
1325 1330 1335Phe Gly Arg Thr Ile Gln Thr Ala Lys Arg Glu Asp Thr Arg Trp1340 1345 1350Ala Ile Ala Glu Arg Val Asp Tyr Asp Gly Thr Gly Ala Val Ile1355 1360 1365Arg Ser Phe Gln Pro Phe Tyr Leu Asp Asp Trp Ash Tyr Val Gly1370 1375 1380Glu Glu Ala Val Ser Ser Ser Met Tyr Ala Thr Ile Tyr Tyr Tyr1385 1390 1395Asp Ala Leu Ala Arg Gln Leu Arg Met Val Asn Ala Lys Gly Tyr1400 1405 1410Glu Arg Arg Thr Ala Phe Tyr Pro Trp Phe Thr Val Asn Glu Asp1415 1420 1425Glu Asn Asp Thr Met Asp Ser Ser Leu Phe Ala Ser Pro Pro Ala1430 1435 1440Arg<210>41<211>2793<212>DNA<213>类芽孢杆菌DAS1529株<400>41atgaacacaa cgtccatata taggggcacg cctacgattt cagttgtgga taaccggaac 60ttggagattc gcattcttca gtataaccgt atcgcggctg aagatccggc agatgagtgt120
atcctgcgga acacgtatac gccgttaagc tatcttggca gcagcatgga tccccgtttg 180ttctcgcaat atcaggatga tcgcggaaca ccgccgaata tacgaaccat ggcttccctg 240agaggcgaag cgctgtgttc ggaaagtgtg gatgccggcc gcaaggcgga gctttttgat 300atcgaggggc ggcccgtctg gcttatcgat gccaacggca cagagacgac tctcgaatat 360gatgtcttag gcaggccaac agccgtattc gagcaacagg aaggtacgga ctccccccag 420tgcagggagc ggtttattta tggtgagaag gaggcggatg cccaggccaa caatttgcgc 480ggacaactgg ttcgccacta cgataccgcg ggccggatac agaccgacag catctccttg 540gctggactgc cgttgcgcca aagccgtcaa ctgctgaaaa attgggatga acctggcgac 600tggagtatgg atgaggaaag cgcctgggcc tcgttgctgg ctgccgaagc ttatgatacg 660agctggcggt atgacgcgca ggacagggtg ctcgcccaaa ccgacgccaa agggaatctc 720cagcaactga cttacaatga cgccggccag ccgcaggcgg tcagcctcaa gctgcaaggc 780caagcggagc aacggatttg gaaccggatc gagtacaacg cggcgggtca agtggatctc 840gccgaagccg ggaatggaat cgtaacggaa tatacttacg aggaaagcac gcagcggtta 900atccgaaaaa aagattcccg cggactgtcc tccggggaaa gagaagtgct gcaggattat 960cgttatgaat atgatccggt aggcaatatc ctttctattt acaatgaagc ggagccggtt1020cgttatttcc gcaatcaggc cgttgctccg aaaaggcaat atgcctacga tgccttgtat1080cagcttgtat ctagttcggg gcgggaatcc gacgcgcttc ggcagcagac gtcgcttcct1140cccttgatca cgcctatccc tctggacgat agccaatacg tcaattacgc tgaaaaatac1200agctatgatc aggcgggcaa tttaatcaag cttagccata acggggcaag tcaatataca1260acgaatgtgt atgtggacaa aagctcaaac cgggggattt ggcggcaagg ggaagacatc1320ccggatatcg cggcttcctt tgacagagca ggcaatcaac aagctttatt cccggggaga1380ccgttggaat gggatacacg caatcaatta agccgtgtcc atatggtcgt gcgcgaaggc1440
ggagacaacg actgggaagg ctatctctat gacagctcgg gaatgcgtat cgtaaaacga1500tctacccgca aaacacagac aacgacgcaa acggatacga ccctctattt gccgggcctg1560gagctgcgaa tccgccagac cggggaccgg gtcacggaag cattgcaggt cattaccgtg1620gatgagggag cgggacaagt gagggtgctg cactgggagg atggaaccga gccgggcggc1680atcgccaatg atcagtaccg gtacagcctg aacgatcatc ttacctcctc tttattggaa1740gttgacgggc aaggtcagat cattagtaag gaagaatttt atccctatgg cggcacagcc1800ctgtggacag cccggtcaga ggtagaggca agctacaaga ccatccgcta ttcaggcaaa1860gagcgggatg ccacaggcct gtattattac ggacaccgct actatatgcc atggttgggt1920cgctggctga atccggaccc ggccggaatg gtagatggac taaacctgta ccgtatggtc1980aggaacaatc ctataggact gatggatccg aatgggaatg cgccaatcaa cgtggcggat2040tatagcttcg tgcatggtga tttagtttat ggtcttagta aggaaagagg aagatatcta2100aagctattta atccaaactt taatatggaa aaatcagact ctcctgctat ggttatagat2160caatataata ataatgttgc attgagtata actaaccaat ataaagtaga agaattgatg2220aaatttcaaa aagacccaca aaaagccgca cggaaaataa aggttccaga agggaatcgt2280ttatcgagga acgaaaatta tcctttgtgg cacgattata ttaacattgg agaagctaaa2340gctgcattta aggcctctca tattttccaa gaagtgaagg ggaattatgg gaaagattat2400tatcataaat tattattaga cagaatgata gaatcgccgt tgctgtggaa acgaggcagc2460aaactcgggc tagaaatcgc cgctaccaat cagagaacaa aaatacactt tgttcttgac2520aatttaaata tcgagcaggt ggttacgaaa gagggtagcg gcggtcagtc aatcacagct2580tcggagctcc gttatattta tcgaaatcgc gaaagattga acgggcgtgt cattttctat2640agaaataatg aaaggctaga tcaggctcca tggcaagaaa atccggactt atggagcaaa2700tatcaaccgg gtcttagaca aagcagcagt tcaagagtca aagaacgagg gattgggaac2760
tttttccgcc ggttttcaat gaagagaaag tag 2793<210>42<211>930<212>PRT<213>类芽孢杆菌DAS1529株<400>42Met Asn Thr Thr Ser Ile Tyr Arg Gly Thr Pro Thr Ile Ser Val Val1 5 10 15Asp Asn Arg Asn Leu Glu Ile Arg Ile Leu Gln Tyr Asn Arg Ile Ala20 25 30Ala Glu Asp Pro Ala Asp Glu Cys Ile Leu Arg Asn Thr Tyr Thr Pro35 40 45Leu Ser Tyr Leu Gly Ser Ser Met Asp Pro Arg Leu Phe Ser Gln Tyr50 55 60Gln Asp Asp Arg Gly Thr Pro Pro Asn Ile Arg Thr Met Ala Ser Leu65 70 75 80Arg Gly Glu Ala Leu Cys Ser Glu Ser Val Asp Ala Gly Arg Lys Ala85 90 95Glu Leu Phe Asp Ile Glu Gly Arg Pro Val Trp Leu Ile Asp Ala Asn100 105 110Gly Thr Glu Thr Thr Leu Glu Tyr Asp Val Leu Gly Arg Pro Thr Ala115 120 125Val Phe Glu Gln Gln Glu Gly Thr Asp Ser Pro Gln Cys Arg Glu Arg
130 135 140Phe Ile Tyr Gly Glu Lys Glu Ala Asp Ala Gln Ala Asn Asn Leu Arg145 150 155 160Gly Gln Leu Val Arg His Tyr Asp Thr Ala Gly Arg Ile Gln Thr Asp165 170 175Ser Ile Ser Leu Ala Gly Leu Pro Leu Arg Gln Ser Arg Gln Leu Leu180 185 190Lys Asn Trp Asp Glu Pro Gly Asp Trp Ser Met Asp Glu Glu Ser Ala195 200 205Trp Ala Ser Leu Leu Ala Ala Glu Ala Tyr Asp Thr Ser Trp Arg Tyr210 215 220Asp Ala Gln Asp Arg Val Leu Ala Gln Thr Asp Ala Lys Gly Asn Leu225 230 235 240Gln Gln Leu Thr Tyr Asn Asp Ala Gly Gln Pro Gln Ala Val Ser Leu245 250 255Lys Leu Gln Gly Gln Ala Glu Gln Arg Ile Trp Asn Arg Ile Glu Tyr260 265 270Asn Ala Ala Gly Gln Val Asp Leu Ala Glu Ala Gly Asn Gly Ile Val275 280 285Thr Glu Tyr Thr Tyr Glu Glu Ser Thr Gln Arg Leu Ile Arg Lys Lys290 295 300Asp Ser Arg Gly Leu Ser Ser Gly Glu Arg Glu Val Leu Gln Asp Tyr
305 310 315 320Arg Tyr Glu Tyr Asp Pro Val Gly Asn Ile Leu Ser Ile Tyr Asn Glu325 330 335Ala Glu Pro Val Arg Tyr Phe Arg Asn Gln Ala Val Ala Pro Lys Arg340 345 350Gln Tyr Ala Tyr Asp Ala Leu Tyr Gln Leu Val Ser Ser Ser Gly Arg355 360 365Glu Ser Asp Ala Leu Arg Gln Gln Thr Ser Leu Pro Pro Leu Ile Thr370 375 380Pro Ile Pro Leu Asp Asp Ser Gln Tyr Val Asn Tyr Ala Glu Lys Tyr385 390 395 400Ser Tyr Asp Gln Ala Gly Asn Leu Ile Lys Leu Ser His Asn Gly Ala405 410 415Ser Gln Tyr Thr Thr Asn Val Tyr Val Asp Lys Ser Ser Asn Arg Gly420 425 430Ile Trp Arg Gln Gly Glu Asp Ile Pro Asp Ile Ala Ala Ser Phe Asp435 440 445Arg Ala Gly Asn Gln Gln Ala Leu Phe Pro Gly Arg Pro Leu Glu Trp450 455 460Asp Thr Arg Asn Gln Leu Ser Arg Val His Met Val Val Arg Glu Gly465 470 475 480Gly Asp Asn Asp Trp Glu Gly Tyr Leu Tyr Asp Ser Ser Gly Met Arg
485 490 495Ile Val Lys Arg Ser Thr Arg Lys Thr Gln Thr Thr Thr Gln Thr Asp500 505 510Thr Thr Leu Tyr Leu Pro Gly Leu Glu Leu Arg Ile Arg Gln Thr Gly515 520 525Asp Arg Val Thr Glu Ala Leu Gln Val Ile Thr Val Asp Glu Gly Ala530 535 540Gly Gln Val Arg Val Leu His Trp Glu Asp Gly Thr Glu Pro Gly Gly545 550 555 560Ile Ala Asn Asp Gln Tyr Arg Tyr Ser Leu Asn Asp His Leu Thr Ser565 570 575Ser Leu Leu Glu Val Asp Gly Gln Gly Gln Ile Ile Ser Lys Glu Glu580 585 590Phe Tyr Pro Tyr Gly Gly Thr Ala Leu Trp Thr Ala Arg Ser Glu Val595 600 605Glu Ala Ser Tyr Lys Thr Ile Arg Tyr Ser Gly Lys Glu Arg Asp Ala610 615 620Thr Gly Leu Tyr Tyr Tyr Gly His Arg Tyr Tyr Met Pro Trp Leu Gly625 630 635 640Arg Trp Leu Asn Pro Asp Pro Ala Gly Met Val Asp Gly Leu Asn Leu645 650 655Tyr Arg Met Val Arg Asn Asn Pro Ile Gly Leu Met Asp Pro Asn Gly
660 665 670Asn Ala Pro Ile Asn Val Ala Asp Tyr Ser Phe Val His Gly Asp Leu675 680 685Val Tyr Gly Leu Ser Lys Glu Arg Gly Arg Tyr Leu Lys Leu Phe Asn690 695 700Pro Asn Phe Asn Met Glu Lys Ser Asp Ser Pro Ala Met Val Ile Asp705 710 715 720Gln Tyr Asn Asn Asn Val Ala Leu Ser Ile Thr Asn Gln Tyr Lys Val725 730 735Glu Glu Leu Met Lys Phe Gln Lys Asp Pro Gln Lys Ala Ala Arg Lys740 745 750Ile Lys Val Pro Glu Gly Asn Arg Leu Ser Arg Asn Glu Asn Tyr Pro755 760 765Leu Trp His Asp Tyr Ile Asn Ile Gly Glu Ala Lys Ala Ala Phe Lys770 775 780Ala Ser His Ile Phe Gln Glu Val Lys Gly Asn Tyr Gly Lys Asp Tyr785 790 795 800Tyr His Lys Leu Leu Leu Asp Arg Met Ile Glu Ser Pro Leu Leu Trp805 810 815Lys Arg Gly Ser Lys Leu Gly Leu Glu Ile Ala Ala Thr Asn Gln Arg820 825 830Thr Lys Ile His Phe Val Leu Asp Asn Leu Asn Ile Glu Gln Val Val
835 840 845Thr Lys Glu Gly Ser Gly Gly Gln Ser Ile Thr Ala Ser Glu Leu Arg850 855 860Tyr Ile Tyr Arg Asn Arg Glu Arg Leu Asn Gly Arg Val Ile Phe Tyr865 870 875 880Arg Asn Asn Glu Arg Leu Asp Gln Ala Pro Trp Gln Glu Asn Pro Asp885 890 895Leu Trp Ser Lys Tyr Gln Pro Gly Leu Arg Gln Ser Set Ser Ser Arg900 905 910Val Lys Glu Arg Gly Ile Gly Ash Phe Phe Arg Arg Phe Ser Met Lys915 920 925Arg Lys930<210>43<211>953<212>PRT<213>类芽孢杆菌DAS1529株<400>43Met Lys Met Ile Pro Trp Thr His His Tyr Leu Leu His Arg Leu Arg1 5 10 15Gly Glu Met Glu Val Lys Pro Met Asn Thr Thr Ser Ile Tyr Arg Gly20 25 30Thr Pro Thr Ile Ser Val Val Asp Asn Arg Asn Leu Glu Ile Arg Ile35 40 45
Leu Gln Tyr Asn Arg Ile Ala Ala Glu Asp Pro Ala Asp Glu Cys Ile50 55 60Leu Arg Asn Thr Tyr Thr Pro Leu Ser Tyr Leu Gly Ser Ser Met Asp65 70 75 80Pro Arg Leu Phe Ser Gln Tyr Gln Asp Asp Arg Gly Thr Pro Pro Asn85 90 95Ile Arg Thr Met Ala Ser Leu Arg Gly Glu Ala Leu Cys Ser Glu Ser100 105 110Val Asp Ala Gly Arg Lys Ala Glu Leu Phe Asp Ile Glu Gly Arg Pro115 120 125Val Trp Leu Ile Asp Ala Asn Gly Thr Glu Thr Thr Leu Glu Tyr Asp130 135 140Val Leu Gly Arg Pro Thr Ala Val Phe Glu Gln Gln Glu Gly Thr Asp145 150 155 160Ser Pro Gln Cys Arg Glu Arg Phe Ile Tyr Gly Glu Lys Glu Ala Asp165 170 175Ala Gln Ala Asn Asn Leu Arg Gly Gln Leu Val Arg His Tyr Asp Thr180 185 190Ala Gly Arg Ile Gln Thr Asp Ser Ile Ser Leu Ala Gly Leu Pro Leu195 200 205Arg Gln Ser Arg Gln Leu Leu Lys Asn Trp Asp Glu Pro Gly Asp Trp210 215 220
Ser Met Asp Glu Glu Ser Ala Trp Ala Ser Leu Leu Ala Ala Glu Ala225 230 235 240Tyr Asp Thr Ser Trp Arg Tyr Asp Ala Gln Asp Arg Val Leu Ala Gln245 250 255Thr Asp Ala Lys Gly Asn Leu Gln Gln Leu Thr Tyr Asn Asp Ala Gly260 265 270Gln Pro Gln Ala Val Ser Leu Lys Leu Gln Gly Gln Ala Glu Gln Arg275 280 285Ile Trp Asn Arg Ile Glu Tyr Asn Ala Ala Gly Gln Val Asp Leu Ala290 295 300Glu Ala Gly Asn Gly Ile Val Thr Glu Tyr Thr Tyr Glu Glu Ser Thr305 310 315 320Gln Arg Leu Ile Arg Lys Lys Asp Ser Arg Gly Leu Ser Ser Gly Glu325 330 335Arg Glu Val Leu Gln Asp Tyr Arg Tyr Glu Tyr Asp Pro Val Gly Asn340 345 350Ile Leu Ser Ile Tyr Asn Glu Ala Glu Pro Val Arg Tyr Phe Arg Asn355 360 365Gln Ala Val Ala Pro Lys Arg Gln Tyr Ala Tyr Asp Ala Leu Tyr Gln370 375 380Leu Val Ser Ser Ser Gly Arg Glu Ser Asp Ala Leu Arg Gln Gln Thr385 390 395 400
Ser Leu Pro Pro Leu Ile Thr Pro Ile Pro Leu Asp Asp Ser Gln Tyr405 410 415Val Asn Tyr Ala Glu Lys Tyr Ser Tyr Asp Gln Ala Gly Asn Leu Ile420 425 430Lys Leu Ser Hi s Asn Gly Ala Ser Gln Tyr Thr Thr Asn Val Tyr Val435 440 445Asp Lys Ser Ser Asn Arg Gly Ile Trp Arg Gln Gly Glu Asp Ile Pro450 455 460Asp Ile Ala Ala Ser Phe Asp Arg Ala Gly Asn Gln Gln Ala Leu Phe465 470 475 480Pro Gly Arg Pro Leu Glu Trp Asp Thr Arg Asn Gln Leu Ser Arg Val485 490 495His Met Val Val Arg Glu Gly Gly Asp Asn Asp Trp Glu Gly Tyr Leu500 505 510Tyr Asp Ser Ser Gly Met Arg Ile Val Lys Arg Ser Thr Arg Lys Thr515 520 525Gln Thr Thr Thr Gln Thr Asp Thr Thr Leu Tyr Leu Pro Gly Leu Glu530 535 540Leu Arg Ile Arg Gln Thr Gly Asp Arg Val Thr Glu Ala Leu Gln Val545 550 555 560Ile Thr Val Asp Glu Gly Ala Gly Gln Val Arg Val Leu His Trp Glu565 570 575
Asp Gly Thr Glu Pro Gly Gly Ile Ala Asn Asp Gln Tyr Arg Tyr Ser580 585 590Leu Asn Asp His Leu Thr Ser Ser Leu Leu Glu Val Asp Gly Gln Gly595 600 605Gln Ile Ile Ser Lys Glu Glu Phe Tyr Pro Tyr Gly Gly Thr Ala Leu610 615 620Trp Thr Ala Arg Ser Glu Val Glu Ala Ser Tyr Lys Thr Ile Arg Tyr625 630 635 640Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly His Arg645 650 655Tyr Tyr Met Pro Trp Leu Gly Arg Trp Leu Asn Pro Asp Pro Ala Gly660 665 670Met Val Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Ile675 680 685Gly Leu Met Asp Pro Asn Gly Asn Ala Pro Ile Asn Val Ala Asp Tyr690 695 700Ser Phe Val His Gly Asp Leu Val Tyr Gly Leu Ser Lys Glu Arg Gly705 710 715 720Arg Tyr Leu Lys Leu Phe Asn Pro Asn Phe Asn Met Glu Lys Ser Asp725 730 735Ser Pro Ala Met Val Ile Asp Gln Tyr Asn Asn Asn Val Ala Leu Ser740 745 750
Ile Thr Asn Gln Tyr Lys Val Glu Glu Leu Met Lys Phe Gln Lys Asp755 760 765Pro Gln Lys Ala Ala Arg Lys Ile Lys Val Pro Glu Gly Asn Arg Leu770 775 780Ser Arg Asn Glu Asn Tyr Pro Leu Trp His Asp Tyr Ile Asn Ile Gly785 790 795 800Glu Ala Lys Ala Ala Phe Lys Ala Ser His Ile Phe Gln Glu Val Lys805 810 815Gly Asn Tyr Gly Lys Asp Tyr Tyr His Lys Leu Leu Leu Asp Arg Met820 825 830Ile Glu Ser Pro Leu Leu Trp Lys Arg Gly Ser Lys Leu Gly Leu Glu835 840 845Ile Ala Ala Thr Asn Gln Arg Thr Lys Ile His Phe Val Leu Asp Asn850 855 860Leu Asn Ile Glu Gln Val Val Thr Lys Glu Gly Ser Gly Gly Gln Ser865 870 875 880Ile Thr Ala Ser Glu Leu Arg Tyr Ile Tyr Arg Asn Arg Glu Arg Leu885 890 895Asn Gly Arg Val Ile Phe Tyr Arg Asn Asn Glu Arg Leu Asp Gln Ala900 905 910Pro Trp Gln Glu Asn Pro Asp Leu Trp Ser Lys Tyr Gln Pro Gly Leu915 920 925
Arg Gln Ser Ser Ser Ser Arg Val Lys Glu Arg Gly Ile Gly Asn Phe930 935 940Phe Arg Arg Phe Ser Met Lys Arg Lys945 950<210>44<211>4425<212>DNA<213>发光光杆状菌<220>
<221>CDS<222>(1)..(4422)<400>44atg caa aat tca caa gat ttt agt att acg gaa ctg tca ctg ccc aaa48Met Gln Asn Ser Gln Asp Phe Ser Ile Thr Glu Leu Ser Leu Pro Lys15 10 15ggg ggg ggc gct atc acg gga atg ggt gaa gca tta acc ccc act gga96Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Ala Leu Thr Pro Thr Gly20 25 30ccg gat ggt atg gcc gcg cta tct cta cca ttg cct att tct gcc ggg 144Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Leu Pro Ile Ser Ala Gly35 40 45cgc ggt tat gct ccc gca ttc act ctg aat tac aac agc ggc gcc ggt 192Arg Gly Tyr Ala Pro Ala Phe Thr Leu Asn Tyr Asn Ser Gly Ala Gly50 55 60aac agt cca ttt ggt ctg ggt tgg gat tgc aac gtt atg act atc cgc 240Ash Ser Pro Phe Gly Leu Gly Trp Asp Cys Asn Val Met Thr Ile Arg65 70 75 80cgc cgc acc cat ttt ggc gtc ccc cat tat gac gaa acc gat acc ttt 288Arg Arg Thr His Phe Gly Val Pro His Tyr Asp Glu Thr Asp Thr Phe85 90 95
ttg ggg cca gaa ggc gaa gtg ctg gtg gta gcg gat caa cct cgc gac336Leu Gly Pro Glu Gly Glu Val Leu Val Val Ala Asp Gln Pro Arg Asp100 105 110gaa tcc aca tta cag ggt atc aat tta ggc gcc acc ttt acc gtt acc384Glu Ser Thr Leu Gln Gly Ile Asn Leu Gly Ala Thr Phe Thr Val Thr115 120 125ggc tac cgt tcc cgt ctg gaa agc cat ttc agc cga ttg gaa tat tgg432Gly Tyr Arg Ser Arg Leu Glu Ser His Phe Ser Arg Leu Glu Tyr Trp130 135 140caa ccc aaa aca aca ggt aaa aca gat ttt tgg ttg ata tat agc cca480Gln Pro Lys Thr Thr Gly Lys Thr Asp Phe Trp Leu Ile Tyr Ser Pro145 150 155 160gat ggg cag gtg cat cta ctg ggt aaa tca ccg caa gcg cgg atc agc528Asp Gly Gln Val His Leu Leu Gly Lys Ser Pro Gln Ala Arg Ile Ser165 170 175aac cca tcc caa acg aca caa aca gca caa tgg ctg ctg gaa gcc tct576Asn Pro Ser Gln Thr Thr Gln Thr Ala Gln Trp Leu Leu Glu Ala Ser180 185 190gta tca tca cgt ggc gaa caa att tat tat caa tat cgc gcc gaa gat624Val Ser Ser Arg Gly Glu Gln Ile Tyr Tyr Gln Tyr Arg Ala Glu Aspl95 200 205gac aca ggt tgc gaa gca gat gaa att acg cac cat tta cag gct aca672Asp Thr Gly Cys Glu Ala Asp Glu Ile Thr His His Leu Gln Ala Thr210 215 220gcg caa cgt tat tta cac atc gtg tat tac ggc aac cgt aca gcc agc720Ala Gln Arg Tyr Leu His Ile Val Tyr Tyr Gly Asn Arg Thr Ala Ser225 230 235 240gaa aca tta ccc ggt ctg gat ggc agc gcc cca tca caa gca gac tgg768Glu Thr Leu Pro Gly Leu Asp Gly Ser Ala Pro Ser Gln Ala Asp Trp245 250 255ttg ttc tat ctg gta ttt gat tac ggc gaa cgc agt aac aac ctg aaa816Leu Phe Tyr Leu Val Phe Asp Tyr Gly Glu Arg Ser Asn Asn Leu Lys260 265 270
acg cca cca gca ttt tcg act aca ggt agc tgg ctt tgc cgt cag gac 864Thr Pro Pro Ala Phe Ser Thr Thr Gly Ser Trp Leu Cys Arg Gln Asp275 280 285cgt ttt tcc cgt tat gaa tat ggc ttt gag att cgt acc cgc cgc tta 912Arg Phe Ser Arg Tyr Glu Tyr Gly Phe Glu Ile Arg Thr Arg Arg Leu290 295 300tgc cgt cag gta ttg atg tac cat cac ctg caa gca ctg gat agt aag 960Cys Arg Gln Val Leu Met Tyr His His Leu Gln Ala Leu Asp Ser Lys305 310 315 320ata aca gaa cac aac gga cca acg ctg gtt tca cgc ctg ata ctc aat1008Ile Thr Glu His Asn Gly Pro Thr Leu Val Ser Arg Leu Ile Leu Asn325 330 335tac gac gaa agc gcg ata gcc agc acg cta gta ttc gtt cgc cga gtg1056Tyr Asp Glu Ser Ala Ile Ala Ser Thr Leu Val Phe Val Arg Arg Val340 345 350gga cac gag caa gat ggt aat gtc gtc acc ctg ccg cca tta gaa ttg1104Gly His Glu Gln Asp Gly Asn Val Val Thr Leu Pro Pro Leu Glu Leu355 360 365gca tat cag gat ttt tca ccg cga cat cac gct cac tgg caa cca atg1152Ala Tyr Gln Asp Phe Ser Pro Arg His His Ala His Trp Gln Pro Met370 375 380gat gta ctg gca aac ttc aat gcc att cag cgc tgg cag cta gtc gat1200Asp Val Leu Ala Asn Phe Asn Ala Ile Gln Arg Trp Gln Leu Val Asp385 390 395 400cta aaa ggc gaa gga tta ccc ggc ctg tta tat cag gat aaa ggc gct1248Leu Lys Gly Glu Gly Leu Pro Gly Leu Leu Tyr Gln Asp Lys Gly Ala405 410 415tgg tgg tac cgc tcc gca cag cgt ctg ggc gaa att ggc tca gat gcc1296Trp Trp Tyr Arg Ser Ala Gln Arg Leu Gly Glu Ile Gly Ser Asp Ala420 425 430gtc act tgg gaa aag atg caa cct tta tcg gtt att cct tct ttg caa1344Val Thr Trp Glu Lys Met Gln Pro Leu Ser Val Ile Pro Ser Leu Gln435 440 445
agt aat gcc tcg ttg gtg gat atc aat gga gac ggc caa ctt gac tgg1392Ser Asn Ala Ser Leu Val Asp Ile Asn Gly Asp Gly Gln Leu Asp Trp450 455 460gtt atc acc gga ccg gga tta cgg gga tat cat agt caa cgc ccg gat1440Val Ile Thr Gly Pro Gly Leu Arg Gly Tyr His Ser Gln Arg Pro Asp465 470 475 480ggc agt tgg aca cgt ttt acc cca ctc aac gct ctg ccg gtg gaa tac1488Gly Ser Trp Thr Arg Phe Thr Pro Leu Asn Ala Leu Pro Val Glu Tyr485 490 495acc cat cca cgc gcg caa ctc gca gat tta atg gga gcc ggg cta tcc1536Thr His Pro Arg Ala Gln Leu Ala Asp Leu Met Gly Ala Gly Leu Ser500 505 510gat ttg gtg ctg atc ggc cct aag agc gtg cgt tta tat gcc aat acc1584Asp Leu Val Leu Ile Gly Pro Lys Ser Val Arg Leu Tyr Ala Asn Thr515 520 525cgc gac ggc ttt gcc aaa gga aaa gat gtg gtg caa tcc ggt gat atc1632Arg Asp Gly Phe Ala Lys Gly Lys Asp Val Val Gln Ser Gly Asp Ile530 535 540aca ctg ccg gtg ccg ggc gcc gat cca cgt aag ttg gtg gcg ttt agt1680Thr Leu Pro Val Pro Gly Ala Asp Pro Arg Lys Leu Val Ala Phe Ser545 550 555 560gat gta ttg ggt tca ggt caa gcc cat ctg gtt gaa gta agc gcg act1728Asp Val Leu Gly Ser Gly Gln Ala His Leu Val Glu Val Ser Ala Thr565 570 575aaa gtc acc tgc tgg cct aat ctg ggg cgc gga cgt ttt ggt caa ccc1776Lys Val Thr Cys Trp Pro Asn Leu Gly Arg Gly Arg Phe Gly Gln Pro580 585 590att acc tta ccg gga ttc agc cag cca gca acc gag ttt aac ccg gct1824Ile Thr Leu Pro Gly Phe Ser Gln Pro Ala Thr Glu Phe Asn Pro Ala595 600 605caa gtt tat ctg gcc gat ctg gat ggc agc ggt cca acg gat ctg att1872Gln Val Tyr Leu Ala Asp Leu Asp Gly Ser Gly Pro Thr Asp Leu Ile610 615 620
tat gtt cat aca aac cgt ctg gat atc ttc ctg aac aaa agt ggc aat1920Tyr Val His Thr Asn Arg Leu Asp Ile Phe Leu Asn Lys Ser Gly Asn625 630 635 640ggc ttt gct gaa cca gtg aca tta cgc ttc ccg gaa ggt ctg cgt ttt1968Gly Phe Ala Glu Pro Val Thr Leu Arg Phe Pro Glu Gly Leu Arg Phe645 650 655gat cat acc tgt cag tta caa atg gcc gat gta caa gga tta ggc gtc2016Asp His Thr Cys Gln Leu Gln Met Ala Asp Val Gln Gly Leu Gly Val660 665 670gcc agc ctg ata ctg agc gtg ccg cat atg tct ccc cat cac tgg cgc2064Ala Ser Leu Ile Leu Ser Val Pro His Met Ser Pro His His Trp Arg675 680 685tgc gat ctg acc aac atg aag ccg tgg tta ctc aat gaa atg aac aac2112Cys Asp Leu Thr Asn Met Lys Pro Trp Leu Leu Asn Glu Met Asn Asn690 695 700aat atg ggg gtc cat cac acc ttg cgt tac cgc agt tcc tcc caa ttc2160Asn Met Gly Val His His Thr Leu Arg Tyr Arg Ser Ser Ser Gln Phe705 710 715 720tgg ctg gat gaa aaa gcc gcg gcg ctg act acc gga caa aca ccg gtt2208Trp Leu Asp Glu Lys Ala Ala Ala Leu Thr Thr Gly Gln Thr Pro Val725 730 735tgc tat ctc ccc ttc ccg atc cac acc cta tgg caa acg gaa aca gaa2256Cys Tyr Leu Pro Phe Pro Ile His Thr Leu Trp Gln Thr Glu Thr Glu740 745 750gat gaa atc agc ggc aac aaa tta gtc aca aca ctt cgt tat gct cgt2304Asp Glu Ile Ser Gly Asn Lys Leu Val Thr Thr Leu Arg Tyr Ala Arg755 760 765ggc gca tgg gac gga cgc gag cgg gaa ttt cgc gga ttt ggt tat gta2352Gly Ala Trp Asp Gly Arg Glu Arg Glu Phe Arg Gly Phe Gly Tyr Val770 775 780gag cag aca gac agc cat caa ctg gct caa ggc aac gcg cca gaa cgt2400Glu Gln Thr Asp Ser His Gln Leu Ala Gln Gly Asn Ala Pro Glu Arg785 790 795 800
acg cca ccg gcg ctg acc aaa aac tgg tat gcc acc gga ctg ccg gtg2448Thr Pro Pro Ala Leu Thr Lys Asn Trp Tyr Ala Thr Gly Leu Pro Val805 810 815ata gat aac gca tta tca acc gag tat tgg cgt gat gat cag gct ttt2496Ile Asp Asn Ala Leu Ser Thr Glu Tyr Trp Arg Asp Asp Gln Ala Phe820 825 830gcc ggt ttc tca ccg cgc ttt acg act tgg caa gat aac aaa gat gtc2544Ala Gly Phe Ser Pro Arg Phe Thr Thr Trp Gln Asp Asn Lys Asp Val835 840 845ccg tta aca ccg gaa gat gat aac agt cgt tac tgg ttc aac cgc gcg2592Pro Leu Thr Pro Glu Asp Asp Asn Ser Arg Tyr Trp Phe Asn Arg Ala850 855 860ttg aaa ggt caa ctg cta cgt agt gaa ctg tac gga ttg gac gat agt2640Leu Lys Gly Gln Leu Leu Arg Ser Glu Leu Tyr Gly Leu Asp Asp Ser865 870 875 880aca aat aaa cac gtt ccc tat act gtc act gaa ttt cgt tca cag gta2688Thr Asn Lys His Val Pro Tyr Thr Val Thr Glu Phe Arg Ser Gln Val885 890 895cgt cga tta cag cat acc gac agc cga tac cct gta ctt tgg tca tct2736Arg Arg Leu Gln His Thr Asp Ser Arg Tyr Pro Val Leu Trp Ser Ser900 905 910gta gtt gaa agc cgc aac tat cac tac gaa cgt atc gcc agc gac ccg2784Val Val Glu Ser Arg Asn Tyr His Tyr Glu Arg Ile Ala Ser Asp Pro915 920 925caa tgc agt caa aat att acg cta tcc agt gat cga ttt ggt cag ccg2832Gln Cys Ser Gln Asn Ile Thr Leu Ser Ser Asp Arg Phe Gly Gln Pro930 935 940cta aaa cag ctt tcg gta cag tac ccg cgc cgc cag cag cca gca atc2880Leu Lys Gln Leu Ser Val Gln Tyr Pro Arg Arg Gln Gln Pro Ala Ile945 950 955 960aat ctg tat cct gat aca ttg cct gat aag ttg tta gcc aac agc tat2928Asn Leu Tyr Pro Asp Thr Leu Pro Asp Lys Leu Leu Ala Asn Ser Tyr965 970 975
gat gac caa caa cgc caa tta cgg ctc acc tat caa caa tcc agt tgg 2976Asp Asp Gln Gln Arg Gln Leu Arg Leu Thr Tyr Gln Gln Ser Ser Trp980 985 990cat cac ctg acc aac aat acc gtt cga gta ttg gga tta ccg gat agt 3024His His Leu Thr Asn Asn Thr Val Arg Val Leu Gly Leu Pro Asp Ser995 10001005acc cgc agt gat atc ttt act tat ggc gct gaa aat gtg cct gct 3069Thr Arg Ser Asp Ile Phe Thr Tyr Gly Ala Glu Asn Val Pro Ala1010 1015 1020ggt ggt tta aat ctg gaa ctt ctg agt gat aaa aat agc ctg atc 3114Gly Gly Leu Asn Leu Glu Leu Leu Ser Asp Lys Asn Ser Leu Ile1025 1030 1035gcg gac gat aaa cca cgt gaa tac ctc ggt cag caa aaa acc gct 3159Ala Asp Asp Lys Pro Arg Glu Tyr Leu Gly Gln Gln Lys Thr Ala1040 1045 1050tat acc gat gga caa aat aca acg ccg ttg caa aca cca aca cgg 3204Tyr Thr Asp Gly Gln Asn Thr Thr Pro Leu Gln Thr Pro Thr Arg1055 1060 1065caa gcc ctg att gcc ttt acc gaa aca acg gta ttc aac cag tcc 3249Gln Ala Leu Ile Ala Phe Thr Glu Thr Thr Val Phe Asn Gln Ser1070 1075 1080aca tta tca gcg ttt aac gga agc atc ccg tcc gat aaa tta tca 3294Thr Leu Ser Ala Phe Asn Gly Ser Ile Pro Ser Asp Lys Leu Ser1085 1090 1095acg acg ctg gag caa gct gga tat cag caa aca aat tat cta ttc 3339Thr Thr Leu Glu Gln Ala Gly Tyr Gln Gln Thr Asn Tyr Leu Phe1100 1105 1110cct cgc act gga gaa gat aaa gtt tgg gta gcc cat cac ggc tat 3384Pro Arg Thr Gly Glu Asp Lys Val Trp Val Ala His His Gly Tyr1115 1120 1125acc gat tat ggt aca gcg gca cag ttc tgg cgc ccg caa aaa cag 3429Thr Asp Tyr Gly Thr Ala Ala Gln Phe Trp Arg Pro Gln Lys Gln1130 1135 1140
agc aac acc caa ctc acc ggt aaa atc acc ctc atc tgg gat gca3474Ser Asn Thr Gln Leu Thr Gly Lys Ile Thr Leu Ile Trp Asp Ala1145 1150 1155aac tat tgc gtt gtg gta caa acc cgg gat gct gct gga ctg aca3519Asn Tyr Cys Val Val Val Gln Thr Arg Asp Ala Ala Gly Leu Thr1160 1165 1170acc tca gcc aaa tat gac tgg cgt ttt ctg acc ccg gtg caa ctc3564Thr Ser Ala Lys Tyr Asp Trp Arg Phe Leu Thr Pro Val Gln Leu1175 1180 1185acc gat atc aat gac aat cag cac ctt atc aca ctg gat gca ttg3609Thr Asp Ile Asn Asp Asn Gln His Leu Ile Thr Leu Asp Ala Leu1190 1195 1200ggc cga cca atc aca ttg cgc ttt tgg gga act gaa aac ggc aag3654Gly Arg Pro Ile Thr Leu Arg Phe Trp Gly Thr Glu Asn Gly Lys1205 1210 1215atg aca ggt tat tcc tca ccg gaa aaa gca tca ttt tct cca cca3699Met Thr Gly Tyr Ser Ser Pro Glu Lys Ala Ser Phe Ser Pro Pro1220 1225 1230tcc gat gtt aat gcc gct att gag tta aaa aaa ccg ctc cct gta3744Ser Asp Val Asn Ala Ala Ile Glu Leu Lys Lys Pro Leu Pro Val1235 1240 1245gca cag tgt cag gtc tac gca cca gaa agc tgg atg cca gta tta3789Ala Gln Cys Gln Val Tyr Ala Pro Glu Ser Trp Met Pro Val Leu1250 1255 1260agt cag aaa acc ttc aat cga ctg gca gaa caa gat tgg caa aag3834Ser Gln Lys Thr Phe Asn Arg Leu Ala Glu Gln Asp Trp Gln Lys1265 1270 1275tta tat aac gcc cga atc atc acc gaa gat gga cgt atc tgc aca3879Leu Tyr Asn Ala Arg Ile Ile Thr Glu Asp Gly Arg Ile Cys Thr1280 1285 1290ctg gct tat cgc cgc tgg gta caa agc caa aag gca atc cct caa3924Leu Ala Tyr Arg Arg Trp Val Gln Ser Gln Lys Ala Ile Pro Gln1295 1300 1305
ctc att agc ctg tta aac aac gga ccc cgt tta cct cct cac agc3969Leu Ile Ser Leu Leu Asn Asn Gly Pro Arg Leu Pro Pro His Ser1310 1315 1320ctg aca ttg acg acg gat cgt tat gat cac gat cct gag caa cag4014Leu Thr Leu Thr Thr Asp Arg Tyr Asp His Asp Pro Glu Gln Gln1325 1330 1335atc cgt caa cag gtg gta ttc agt gat ggc ttt ggc cgc ttg ctg4059Ile Arg Gln Gln Val Val Phe Ser Asp Gly Phe Gly Arg Leu Leu1340 1345 1350caa gcc gct gcc cga cat gag gca ggc atg gcc cgg caa cgc aat4104Gln Ala Ala Ala Arg His Glu Ala Gly Met Ala Arg Gln Arg Asn1355 1360 1365gaa gac ggc tct ttg att ata aat gtc cag cat act gag aac cgt4149Glu Asp Gly Ser Leu Ile Ile Asn Val Gln His Thr Glu Asn Arg1370 1375 1380tgg gca gtg act gga cga acg gaa tat gac aat aag ggg caa ccg4194Trp Ala Val Thr Gly Arg Thr Glu Tyr Asp Asn Lys Gly Gln Pro1385 1390 1395ata cgt acc tat cag ccc tat ttc ctc aat gac tgg cga tac gtc4239Ile Arg Thr Tyr Gln Pro Tyr Phe Leu Asn Asp Trp Arg Tyr Val1400 1405 1410agc aat gat agt gcc cgg cag gaa aaa gaa gct tat gca gat acc4284Ser Asn Asp Ser Ala Arg Gln Glu Lys Glu Ala Tyr Ala Asp Thr1415 1420 1425cat gtc tat gat ccc ata ggt cga gaa atc aag gtt atc acc gca4329His Val Tyr Asp Pro Ile Gly Arg Glu Ile Lys Val Ile Thr Ala1430 1435 1440aaa ggt tgg ttc cgt cga acc ttg ttc act ccc tgg ttt act gtc4374Lys Gly Trp Phe Arg Arg Thr Leu Phe Thr Pro Trp Phe Thr Val1445 1450 1455aat gaa gat gaa aat gac aca gcc gct gag gtg aag aag gta aag4419Asn Glu Asp Glu Asn Asp Thr Ala Ala Glu Val Lys Lys Val Lys1460 1465 1470
atg taa 4425Met<210>45<211>1474<212>PRT<213>发光光杆状菌<400>45Met Gln Asn Ser Gln Asp Phe Ser Ile Thr Glu Leu Ser Leu Pro Lys1 5 10 15Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Ala Leu Thr Pro Thr Gly20 25 30Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Leu Pro Ile Ser Ala Gly35 40 45Arg Gly Tyr Ala Pro Ala Phe Thr Leu Asn Tyr Asn Ser Gly Ala Gly50 55 60Asn Ser Pro Phe Gly Leu Gly Trp Asp Cys Asn Val Met Thr Ile Arg65 70 75 80Arg Arg Thr His Phe Gly Val Pro His Tyr Asp Glu Thr Asp Thr Phe85 90 95Leu Gly Pro Glu Gly Glu Val Leu Val Val Ala Asp Gln Pro Arg Asp100 105 110Glu Ser Thr Leu Gln Gly Ile Asn Leu Gly Ala Thr Phe Thr Val Thr115 120 125
Gly Tyr Arg Ser Arg Leu Glu Ser His Phe Ser Arg Leu Glu Tyr Trp130 135 140Gln Pro Lys Thr Thr Gly Lys Thr Asp Phe Trp Leu Ile Tyr Ser Pro145 150 155 160Asp Gly Gln Val His Leu Leu Gly Lys Ser Pro Gln Ala Arg Ile Ser165 170 175Asn Pro Ser Gln Thr Thr Gln Thr Ala Gln Trp Leu Leu Glu Ala Ser180 185 190Val Ser Ser Arg Gly Glu Gln Ile Tyr Tyr Gln Tyr Arg Ala Glu Asp195 200 205Asp Thr Gly Cys Glu Ala Asp Glu Ile Thr His His Leu Gln Ala Thr210 215 220Ala Gln Arg Tyr Leu His Ile Val Tyr Tyr Gly Asn Arg Thr Ala Ser225 230 235 240Glu Thr Leu Pro Gly Leu Asp Gly Ser Ala Pro Ser Gln Ala Asp Trp245 250 255Leu Phe Tyr Leu Val Phe Asp Tyr Gly Glu Arg Ser Asn Asn Leu Lys260 265 270Thr Pro Pro Ala Phe Ser Thr Thr Gly Ser Trp Leu Cys Arg Gln Asp275 280 285Arg Phe Ser Arg Tyr Glu Tyr Gly Phe Glu Ile Arg Thr Arg Arg Leu290 295 300
Cys Arg Gln Val Leu Met Tyr His His Leu Gln Ala Leu Asp Ser Lys305 310 315 320Ile Thr Glu His Asn Gly Pro Thr Leu Val Ser Arg Leu Ile Leu Asn325 330 335Tyr Asp Glu Ser Ala Ile Ala Ser Thr Leu Val Phe Val Arg Arg Val340 345 350Gly His Glu Gln Asp Gly Asn Val Val Thr Leu Pro Pro Leu Glu Leu355 360 365Ala Tyr Gln Asp Phe Ser Pro Arg His His Ala His Trp Gln Pro Met370 375 380Asp Val Leu Ala Asn Phe Asn Ala Ile Gln Arg Trp Gln Leu Val Asp385 390 395 400Leu Lys Gly Glu Gly Leu Pro Gly Leu Leu Tyr Gln Asp Lys Gly Ala405 410 415Trp Trp Tyr Arg Ser Ala Gln Arg Leu Gly Glu Ile Gly Ser Asp Ala420 425 430Val Thr Trp Glu Lys Met Gln Pro Leu Ser Val Ile Pro Ser Leu Gln435 440 445Ser Asn Ala Ser Leu Val Asp Ile Asn Gly Asp Gly Gln Leu Asp Trp450 455 460Val Ile Thr Gly Pro Gly Leu Arg Gly Tyr His Ser Gln Arg Pro Asp465 470 475 480
Gly Ser Trp Thr Arg Phe Thr Pro Leu Asn Ala Leu Pro Val Glu Tyr485 490 495Thr His Pro Arg Ala Gln Leu Ala Asp Leu Met Gly Ala Gly Leu Ser500 505 510Asp Leu Val Leu Ile Gly Pro Lys Ser Val Arg Leu Tyr Ala Asn Thr515 520 525Arg Asp Gly Phe Ala Lys Gly Lys Asp Val Val Gln Ser Gly Asp Ile530 535 540Thr Leu Pro Val Pro Gly Ala Asp Pro Arg Lys Leu Val Ala Phe Ser545 550 555 560Asp Val Leu Gly Ser Gly Gln Ala His Leu Val Glu Val Ser Ala Thr565 570 575Lys Val Thr Cys Trp Pro Asn Leu Gly Arg Gly Arg Phe Gly Gln Pro580 585 590Ile Thr Leu Pro Gly Phe Ser Gln Pro Ala Thr Glu Phe Asn Pro Ala595 600 605Gln Val Tyr Leu Ala Asp Leu Asp Gly Ser Gly Pro Thr Asp Leu Ile610 615 620Tyr Val His Thr Asn Arg Leu Asp Ile Phe Leu Asn Lys Ser Gly Asn625 630 635 640Gly Phe Ala Glu Pro Val Thr Leu Arg Phe Pro Glu Gly Leu Arg Phe645 650 655
Asp His Thr Cys Gln Leu Gln Met Ala Asp Val Gln Gly Leu Gly Val660 665 670Ala Ser Leu Ile Leu Ser Val Pro His Met Ser Pro His His Trp Arg675 680 685Cys Asp Leu Thr Asn Met Lys Pro Trp Leu Leu Asn Glu Met Asn Asn690 695 700Asn Met Gly Val His His Thr Leu Arg Tyr Arg Ser Ser Ser Gln Phe705 710 715 720Trp Leu Asp Glu Lys Ala Ala Ala Leu Thr Thr Gly Gln Thr Pro Val725 730 735Cys Tyr Leu Pro Phe Pro Ile His Thr Leu Trp Gln Thr Glu Thr Glu740 745 750Asp Glu Ile Ser Gly Asn Lys Leu Val Thr Thr Leu Arg Tyr Ala Arg755 760 765Gly Ala Trp Asp Gly Arg Glu Arg Glu Phe Arg Gly Phe Gly Tyr Val770 775 780Glu Gln Thr Asp Ser His Gln Leu Ala Gln Gly Asn Ala Pro Glu Arg785 790 795 800Thr Pro Pro Ala Leu Thr Lys Asn Trp Tyr Ala Thr Gly Leu Pro Val805 810 815Ile Asp Asn Ala Leu Ser Thr Glu Tyr Trp Arg Asp Asp Gln Ala Phe820 825 830
Ala Gly Phe Ser Pro Arg Phe Thr Thr Trp Gln Asp Asn Lys Asp Val835 840 845Pro Leu Thr Pro Glu Asp Asp Asn Ser Arg Tyr Trp Phe Asn Arg Ala850 855 860Leu Lys Gly Gln Leu Leu Arg Ser Glu Leu Tyr Gly Leu Asp Asp Ser865 870 875 880Thr Asn Lys His Val Pro Tyr Thr Val Thr Glu Phe Arg Ser Gln Val885 890 895Arg Arg Leu Gln His Thr Asp Ser Arg Tyr Pro Val Leu Trp Ser Ser900 905 910Val Val Glu Ser Arg Asn Tyr His Tyr Glu Arg Ile Ala Ser Asp Pro915 920 925Gln Cys Ser Gln Asn Ile Thr Leu Ser Ser Asp Arg Phe Gly Gln Pro930 935 940Leu Lys Gln Leu Ser Val Gln Tyr Pro Arg Arg Gln Gln Pro Ala Ile945 950 955 960Asn Leu Tyr Pro Asp Thr Leu Pro Asp Lys Leu Leu Ala Asn Ser Tyr965 970 975Asp Asp Gln Gln Arg Gln Leu Arg Leu Thr Tyr Gln Gln Ser Ser Trp980 985 990His His Leu Thr Asn Asn Thr Val Arg Val Leu Gly Leu Pro Asp Ser995 10001005
Thr Arg Ser Asp Ile Phe Thr Tyr Gly Ala Glu Asn Val Pro Ala1010 1015 1020Gly Gly Leu Asn Leu Glu Leu Leu Ser Asp Lys Asn Ser Leu Ile1025 1030 1035Ala Asp Asp Lys Pro Arg Glu Tyr Leu Gly Gln Gln Lys Thr Ala1040 1045 1050Tyr Thr Asp Gly Gln Asn Thr Thr Pro Leu Gln Thr Pro Thr Arg1055 1060 1065Gln Ala Leu Ile Ala Phe Thr Glu Thr Thr Val Phe Asn Gln Ser1070 1075 1080Thr Leu Ser Ala Phe Asn Gly Ser Ile Pro Ser Asp Lys Leu Ser1085 1090 1095Thr Thr Leu Glu Gln Ala Gly Tyr Gln Gln Thr Asn Tyr Leu Phe1100 1105 1110Pro Arg Thr Gly Glu Asp Lys Val Trp Val Ala His His Gly Tyr1115 1120 1125Thr Asp Tyr Gly Thr Ala Ala Gln Phe Trp Arg Pro Gln Lys Gln1130 1135 1140Ser Asn Thr Gln Leu Thr Gly Lys Ile Thr Leu Ile Trp Asp Ala1145 1150 1155Asn Tyr Cys Val Val Val Gln Thr Arg Asp Ala Ala Gly Leu Thr1160 1165 1170
Thr Ser Ala Lys Tyr Asp Trp Arg Phe Leu Thr Pro Val Gln Leu1175 1180 1185Thr Asp Ile Asn Asp Asn Gln His Leu Ile Thr Leu Asp Ala Leu1190 1195 1200Gly Arg Pro Ile Thr Leu Arg Phe Trp Gly Thr Glu Asn Gly Lys1205 1210 1215Met Thr Gly Tyr Ser Ser Pro Glu Lys Ala Ser Phe Ser Pro Pro1220 1225 1230Ser Asp Val Asn Ala Ala Ile Glu Leu Lys Lys Pro Leu Pro Val1235 1240 1245Ala Gln Cys Gln Val Tyr Ala Pro Glu Ser Trp Met Pro Val Leu1250 1255 1260Ser Gln Lys Thr Phe Asn Arg Leu Ala Glu Gln Asp Trp Gln Lys1265 1270 1275Leu Tyr Asn Ala Arg Ile Ile Thr Glu Asp Gly Arg Ile Cys Thr1280 1285 1290Leu Ala Tyr Arg Arg Trp Val Gln Ser Gln Lys Ala Ile Pro Gln1295 1300 1305Leu Ile Ser Leu Leu Asn Asn Gly Pro Arg Leu Pro Pro His Ser1310 1315 1320Leu Thr Leu Thr Thr Asp Arg Tyr Asp His Asp Pro Glu Gln Gln1325 1330 1335
Ile Arg Gln Gln Val Val Phe Ser Asp Gly Phe Gly Arg Leu Leu1340 1345 1350Gln Ala Ala Ala Arg His Glu Ala Gly Met Ala Arg Gln Arg Asn1355 1360 1365Glu Asp Gly Ser Leu Ile Ile Asn Val Gln His Thr Glu Asn Arg1370 1375 1380Trp Ala Val Thr Gly Arg Thr Glu Tyr Asp Asn Lys Gly Gln Pro1385 1390 1395Ile Arg Thr Tyr Gln Pro Tyr Phe Leu Asn Asp Trp Arg Tyr Val1400 1405 1410Ser Asn Asp Ser Ala Arg Gln Glu Lys Glu Ala Tyr Ala Asp Thr1415 1420 1425His Val Tyr Asp Pro Ile Gly Arg Glu Ile Lys Val Ile Thr Ala1430 1435 1440Lys Gly Trp Phe Arg Arg Thr Leu Phe Thr Pro Trp Phe Thr Val1445 1450 1455Asn Glu Asp Glu Asn Asp Thr Ala Ala Glu Val Lys Lys Val Lys1460 1465 1470Met<210>46<211>2883<212>DNA
<213>发光光杆状菌<220>
<221>CDS<222>(1)..(2880)<400>46atg aaa aac att gat ccc aaa ctt tat caa aaa acc cct act gtc agc 48Met Lys Asn Ile Asp Pro Lys Leu Tyr Gln Lys Thr Pro Thr Val Ser1 5 10 15gtt tac gat aac cgt ggt ctg ata atc cgt aac atc gat ttt cat cgt 96Val Tyr Asp Asn Arg Gly Leu Ile Ile Arg Asn Ile Asp Phe His Arg20 25 30act acc gca aat ggt gat ccc gat acc cgt att acc cgc cat caa tac144Thr Thr Ala Asn Gly Asp Pro Asp Thr Arg Ile Thr Arg His Gln Tyr35 40 45gat att cac gga cac cta aat caa agc atc gat ccg cgc cta tat gaa192Asp Ile His Gly His Leu Asn Gln Ser Ile Asp Pro Arg Leu Tyr Glu50 55 60gcc aag caa acc aac aat acg atc aaa ccc aat ttt ctt tgg cag tat240Ala Lys Gln Thr Asn Asn Thr Ile Lys Pro Asn Phe Leu Trp Gln Tyr65 70 75 80gat ttg acc ggt aat ccc cta tgt aca gag agc att gat gca ggt cgc288Asp Leu Thr Gly Asn Pro Leu Cys Thr Glu Ser Ile Asp Ala Gly Arg85 90 95act gtc acc ttg aat gat att gaa ggc cgt ccg cta cta acg gtg act336Thr Val Thr Leu Asn Asp Ile Glu Gly Arg Pro Leu Leu Thr Val Thr100 105 110gca aca ggg gtt ata caa act cga caa tat gaa act tct tcc ctg ccc384Ala Thr Gly Val Ile Gln Thr Arg Gln Tyr Glu Thr Ser Ser Leu Pro115 120 125ggt cgt ctg tta tct gtt gcc gaa caa aca ccc gag gaa aaa aca tcc432Gly Arg Leu Leu Ser Val Ala Glu Gln Thr Pro Glu Glu Lys Thr Ser130 135 140
cgt atc acc gaa cgc ctg att tgg gct ggc aat acc gaa gca gag aaa480Arg Ile Thr Glu Arg Leu Ile Trp Ala Gly Asn Thr Glu Ala Glu Lys145 150 155 160gac cat aac ctt gcc ggc cag tgc gtg cgt cac tat gac acg gcg gga528Asp His Asn Leu Ala Gly Gln Cys Val Arg His Tyr Asp Thr Ala Gly165 170 175gtt acc cgg tta gag agt tta tca ctg acc ggt act gtt tta tct caa576Val Thr Arg Leu Glu Ser Leu Ser Leu Thr Gly Thr Val Leu Ser Gln180 185 190tcc agc caa cta ttg atc gac act caa gag gca aac tgg aca ggt gat624Ser Ser Gln Leu Leu Ile Asp Thr Gln Glu Ala Asn Trp Thr Gly Asp195 200 205aac gaa acc gtc tgg caa aac atg ctg gct gat gac atc tac aca acc672Asn Glu Thr Val Trp Gln Asn Met Leu Ala Asp Asp Ile Tyr Thr Thr210 215 220ctg agc acc ttc gat gcc acc ggt gct tta ctg act cag acc gat gcg720Leu Ser Thr Phe Asp Ala Thr Gly Ala Leu Leu Thr Gln Thr Asp Ala225 230 235 240aaa ggg aac att cag aga ctg gct tat gat gtg gcc ggg cag cta aac768Lys Gly Asn Ile Gln Arg Leu Ala Tyr Asp Val Ala Gly Gln Leu Asn245 250 255ggg agc tgg cta aca ctc aaa ggc cag acg gaa caa gtg att atc aaa816Gly Ser Trp Leu Thr Leu Lys Gly Gln Thr Glu Gln Val Ile Ile Lys260 265 270tcc ctg acc tac tcc gcc gcc gga caa aaa tta cgt gag gaa cac ggc864Ser Leu Thr Tyr Ser Ala Ala Gly Gln Lys Leu Arg Glu Glu His Gly275 280 285aat gat gtt atc acc gaa tac agt tat gaa ccg gaa acc caa cgg ctg912Asn Asp Val Ile Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gln Arg Leu290 295 300atc ggt atc aaa acc cgc cgt ccg tca gac act aaa gtg cta caa gac960Ile Gly Ile Lys Thr Arg Arg Pro Ser Asp Thr Lys Val Leu Gln Asp305 310 315 320
ctg cgc tat gaa tat gac ccg gta ggc aat gtc atc agc atc cgt aat1008Leu Arg Tyr Glu Tyr Asp Pro Val Gly Asn Val Ile Ser Ile Arg Asn325 330 335gac gcg gaa gcc acc cgc ttt tgg cac aat cag aaa gtg atg ccg gaa1056Asp Ala Glu Ala Thr Arg Phe Trp His Asn Gln Lys Val Met Pro Glu340 345 350aac act tat acc tac gat tcc ctg tat cag ctt atc agc gcc acc ggg1104Asn Thr Tyr Thr Tyr Asp Ser Leu Tyr Gln Leu Ile Ser Ala Thr Gly355 360 365cgc gaa atg gcg aat ata ggt caa caa agt cac caa ttt ccc tca ccc1152Arg Glu Met Ala Asn Ile Gly Gln Gln Ser His Gln Phe Pro Ser Pro370 375 380gct cta cct tct gat aac aac acc tat acc aac tat acc cgt act tat1200Ala Leu Pro Ser Asp Asn Asn Thr Tyr Thr Asn Tyr Thr Arg Thr Tyr385 390 395 400act tat gac cgt ggc ggc aat ctg acc aaa atc cag cac agt tca ccg1248Thr Tyr Asp Arg Gly Gly Asn Leu Thr Lys Ile Gln His Ser Ser Pro405 410 415gcg acg caa aac aac tac acc acc aat atc acg gtt tca aat cgc agc1296Ala Thr Gln Asn Asn Tyr Thr Thr Asn Ile Thr Val Ser Asn Arg Ser420 425 430aac cgc gca gta ctc agc aca ttg acc gaa gat ccg gcg caa gta gat1344Asn Arg Ala Val Leu Ser Thr Leu Thr Glu Asp Pro Ala Gln Val Asp435 440 445gct ttg ttt gat gca ggc gga cat cag aac acc ttg ata tca gga caa1392Ala Leu Phe Asp Ala Gly Gly His Gln Asn Thr Leu Ile Ser Gly Gln450 455 460aac ctg aac tgg aat act cgt ggt gaa ctg caa caa gta aca ctg gtt1440Asn Leu Asn Trp Asn Thr Arg Gly Glu Leu Gln Gln Val Thr Leu Val465 470 475 480aaa cgg gac aag ggc gcc aat gat gat cgg gaa tgg tat cgt tat agc1488Lys Arg Asp Lys Gly Ala Asn Asp Asp Arg Glu Trp Tyr Arg Tyr Ser485 490 495
ggt gac gga aga agg atg tta aaa atc aat gaa cag cag gcc agc aac1536Gly Asp Gly Arg Arg Met Leu Lys Ile Asn Glu Gln Gln Ala Ser Asn500 505 510aac gct caa aca caa cgt gtg act tat ttg ccg aac tta gaa ctt cgt1584Asn Ala Gln Thr Gln Arg Val Thr Tyr Leu Pro Asn Leu Glu Leu Arg515 520 525cta aca caa aac agc acg gcc aca acc gaa gat ttg caa gtt atc acc1632Leu Thr Gln Asn Ser Thr Ala Thr Thr Glu Asp Leu Gln Val Ile Thr530 535 540gta ggc gaa gcg ggc cgg gca cag gta cga gta tta cat tgg gag agc1680Val Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp Glu Ser545 550 555 560ggt aaa ccg gaa gat atc gac aat aat cag ttg cgt tat agt tac gat1728Gly Lys Pro Glu Asp Ile Asp Asn Asn Gln Leu Arg Tyr Ser Tyr Asp565 570 575aat ctt atc ggt tcc agt caa ctt gaa tta gat agc gaa gga caa att1776Asn Leu Ile Gly Ser Ser Gln Leu Glu Leu Asp Ser Glu Gly Gln Ile580 585 590atc agt gaa gaa gaa tat tat ccc tat ggt gga aca gca tta tgg gcc1824Ile Ser Glu Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr Ala Leu Trp Ala595 600 605gcc agg aat cag aca gaa gcc agt tat aaa act atc cgt tat tca ggc1872Ala Arg Asn Gln Thr Glu Ala Ser Tyr Lys Thr Ile Arg Tyr Ser Gly610 615 620aaa gag cgg gat gcc acc ggg cta tat tac tac ggc tat cgg tat tac1920Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg Tyr Tyr625 630 635 640caa ccg tgg ata gga cgg tgg tta agc tcc gat ccg gca gga aca atc1968Gln Pro Trp Ile Gly Arg Trp Leu Ser Ser Asp Pro Ala Gly Thr Ile645 650 655gat ggg ctg aat tta tat cgg atg gtg agg aat aat cca gtt acc ctc2016Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Val Thr Leu660 665 670
ctt gat cct gat gga tta atg cca aca att gca gaa cgc ata gca gca2064Leu Asp Pro Asp Gly Leu Met Pro Thr Ile Ala Glu Arg Ile Ala Ala675 680 685cta aaa aaa aat aaa gta aca gac tca gcg cct tcg cca gca aat gcc2112Leu Lys Lys Asn Lys Val Thr Asp Ser Ala Pro Ser Pro Ala Asn Ala690 695 700aca aac gta gcg ata aac atc cgc ccg cct gta gca cca aaa cct agc2160Thr Asn Val Ala Ile Asn Ile Arg Pro Pro Val Ala Pro Lys Pro Ser705 710 715 720tta ccg aaa gca tca acg agt agc caa cca acc aca cac cct atc gga2208Leu Pro Lys Ala Ser Thr Ser Ser Gln Pro Thr Thr His Pro Ile Gly725 730 735gct gca aac ata aaa cca acg acg tct ggg tca tct att gtt gct cca2256Ala Ala Asn Ile Lys Pro Thr Thr Ser Gly Ser Ser Ile Val Ala Pro740 745 750ttg agt cca gta gga aat aaa tct act tct gaa atc tct ctg cca gaa2304Leu Ser Pro Val Gly Asn Lys Ser Thr Ser Glu Ile Ser Leu Pro Glu755 760 765agc gct caa agc agt tct tca agc act acc tcg aca aat cta cag aaa2352Ser Ala Gln Ser Ser Ser Ser Ser Thr Thr Ser Thr Asn Leu Gln Lys770 775 780aaa tca ttt act tta tat aga gca gat aac aga tcc ttt gaa gaa atg2400Lys Ser Phe Thr Leu Tyr Arg Ala Asp Asn Arg Ser Phe Glu Glu Met785 790 795 800caa agt aaa ttc cct gaa gga ttt aaa gcc tgg act cct cta gac act2448Gln Ser Lys Phe Pro Glu Gly Phe Lys Ala Trp Thr Pro Leu Asp Thr805 810 815aag atg gca agg caa ttt gct agt atc ttt att ggt cag aaa gat aca2496Lys Met Ala Arg Gln Phe Ala Ser Ile Phe Ile Gly Gln Lys Asp Thr820 825 830tct aat tta cct aaa gaa aca gtc aag aac ata agc aca tgg gga gca2544Ser Asn Leu Pro Lys Glu Thr Val Lys Asn Ile Ser Thr Trp Gly Ala835 840 845
aag cca aaa cta aaa gat ctc tca aat tac ata aaa tat acc aag gac2592Lys Pro Lys Leu Lys Asp Leu Ser Asn Tyr Ile Lys Tyr Thr Lys Asp850 855 860aaa tct aca gta tgg gtt tct act gca att aat act gaa gca ggt gga2640Lys Ser Thr Val Trp Val Ser Thr Ala Ile Asn Thr Glu Ala Gly Gly865 870 875 880caa agc tca ggg gct cca ctc cat aaa att gat atg gat ctc tac gag2688Gln Ser Ser Gly Ala Pro Leu His Lys Ile Asp Met Asp Leu Tyr Glu885 890 895ttt gcc att gat gga caa aaa cta aat cca cta ccg gag ggt aga act2736Phe Ala Ile Asp Gly Gln Lys Leu Asn Pro Leu Pro Glu Gly Arg Thr900 905 910aaa aac atg gta cct tcc ctt tta ctc gac acc cca caa ata gag aca2784Lys Asn Met Val Pro Ser Leu Leu Leu Asp Thr Pro Gln Ile Glu Thr915 920 925tca tcc atc att gca ctt aat cat gga ccg gta aat gat gca gaa att2832Ser Ser Ile Ile Ala Leu Asn His Gly Pro Val Asn Asp Ala Glu Ile930 935 940tca ttt ctg aca aca att ccg ctt aaa aat gta aaa cct cat aag aga2880Ser Phe Leu Thr Thr Ile Pro Leu Lys Ash Val Lys Pro His Lys Arg945 950 955 960taa2883<210>47<211>960<212>PRT<213>发光光杆状菌<400>47Met Lys Asn Ile Asp Pro Lys Leu Tyr Gln Lys Thr Pro Thr Val Ser1 5 10 15Val Tyr Asp Asn Arg Gly Leu Ile Ile Arg Asn Ile Asp Phe His Arg20 25 30
Thr Thr Ala Asn Gly Asp Pro Asp Thr Arg Ile Thr Arg His Gln Tyr35 40 45Asp Ile His Gly His Leu Asn Gln Ser Ile Asp Pro Arg Leu Tyr Glu50 55 60Ala Lys Gln Thr Asn Asn Thr Ile Lys Pro Asn Phe Leu Trp Gln Tyr65 70 75 80Asp Leu Thr Gly Asn Pro Leu Cys Thr Glu Ser Ile Asp Ala Gly Arg85 90 95Thr Val Thr Leu Asn Asp Ile Glu Gly Arg Pro Leu Leu Thr Val Thr100 105 110Ala Thr Gly Val Ile Gln Thr Arg Gln Tyr Glu Thr Ser Ser Leu Pro115 120 125Gly Arg Leu Leu Ser Val Ala Glu Gln Thr Pro Glu Glu Lys Thr Ser130 135 140Arg Ile Thr Glu Arg Leu Ile Trp Ala Gly Asn Thr Glu Ala Glu Lys145 150 155 160Asp His Asn Leu Ala Gly Gln Cys Val Arg His Tyr Asp Thr Ala Gly165 170 175Val Thr Arg Leu Glu Ser Leu Ser Leu Thr Gly Thr Val Leu Ser Gln180 185 190Ser Ser Gln Leu Leu Ile Asp Thr Gln Glu Ala Asn Trp Thr Gly Asp195 200 205
Asn Glu Thr Val Trp Gln Asn Met Leu Ala Asp Asp Ile Tyr Thr Thr210 215 220Leu Ser Thr Phe Asp Ala Thr Gly Ala Leu Leu Thr Gln Thr Asp Ala225 230 235 240Lys Gly Asn Ile Gln Arg Leu Ala Tyr Asp Val Ala Gly Gln Leu Asn245 250 255Gly Ser Trp Leu Thr Leu Lys Gly Gln Thr Glu Gln Val Ile Ile Lys260 265 270Ser Leu Thr Tyr Ser Ala Ala Gly Gln Lys Leu Arg Glu Glu His Gly275 280 285Asn Asp Val Ile Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gln Arg Leu290 295 300Ile Gly Ile Lys Thr Arg Arg Pro Ser Asp Thr Lys Val Leu Gln Asp305 310 315 320Leu Arg Tyr Glu Tyr Asp Pro Val Gly Asn Val Ile Ser Ile Arg Asn325 330 335Asp Ala Glu Ala Thr Arg Phe Trp His Asn Gln Lys Val Met Pro Glu340 345 350Asn Thr Tyr Thr Tyr Asp Ser Leu Tyr Gln Leu Ile Ser Ala Thr Gly355 360 365Arg Glu Met Ala Asn Ile Gly Gln Gln Ser His Gln Phe Pro Ser Pro370 375 380
Ala Leu Pro Ser Asp Asn Asn Thr Tyr Thr Asn Tyr Thr Arg Thr Tyr385 390 395 400Thr Tyr Asp Arg Gly Gly Asn Leu Thr Lys Ile Gln His Ser Ser Pro405 410 415Ala Thr Gln Asn Asn Tyr Thr Thr Asn Ile Thr Val Ser Asn Arg Ser420 425 430Asn Arg Ala Val Leu Ser Thr Leu Thr Glu Asp Pro Ala Gln Val Asp435 440 445Ala Leu Phe Asp Ala Gly Gly His Gln Asn Thr Leu Ile Ser Gly Gln450 455 460Asn Leu Asn Trp Asn Thr Arg Gly Glu Leu Gln Gln Val Thr Leu Val465 470 475 480Lys Arg Asp Lys Gly Ala Asn Asp Asp Arg Glu Trp Tyr Arg Tyr Ser485 490 495Gly Asp Gly Arg Arg Met Leu Lys Ile Asn Glu Gln Gln Ala Ser Asn500 505 510Asn Ala Gln Thr Gln Arg Val Thr Tyr Leu Pro Asn Leu Glu Leu Arg515 520 525Leu Thr Gln Asn Ser Thr Ala Thr Thr Glu Asp Leu Gln Val Ile Thr530 535 540Val Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp Glu Ser545 550 555 560
Gly Lys Pro Glu Asp Ile Asp Asn Asn Gln Leu Arg Tyr Ser Tyr Asp565 570 575Asn Leu Ile Gly Ser Ser Gln Leu Glu Leu Asp Ser Glu Gly Gln Ile580 585 590Ile Ser Glu Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr Ala Leu Trp Ala595 600 605Ala Arg Asn Gln Thr Glu Ala Ser Tyr Lys Thr Ile Arg Tyr Ser Gly610 615 620Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg Tyr Tyr625 630 635 640Gln Pro Trp Ile Gly Arg Trp Leu Ser Ser Asp Pro Ala Gly Thr Ile645 650 655Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Val Thr Leu660 665 670Leu Asp Pro Asp Gly Leu Met Pro Thr Ile Ala Glu Arg Ile Ala Ala675 680 685Leu Lys Lys Asn Lys Val Thr Asp Ser Ala Pro Ser Pro Ala Asn Ala690 695 700Thr Asn Val Ala Ile Asn Ile Arg Pro Pro Val Ala Pro Lys Pro Ser705 710 715 720Leu Pro Lys Ala Ser Thr Ser Ser Gln Pro Thr Thr His Pro Ile Gly725 730 735
Ala Ala Asn Ile Lys Pro Thr Thr Ser Gly Ser Ser Ile Val Ala Pro740 745 750Leu Ser Pro Val Gly Asn Lys Ser Thr Ser Glu Ile Ser Leu Pro Glu755 760 765Ser Ala Gln Ser Ser Ser Ser Ser Thr Thr Ser Thr Asn Leu Gln Lys770 775 780Lys Ser Phe Thr Leu Tyr Arg Ala Asp Asn Arg Ser Phe Glu Glu Met785 790 795 800Gln Ser Lys Phe Pro Glu Gly Phe Lys Ala Trp Thr Pro Leu Asp Thr805 810 815Lys Met Ala Arg Gln Phe Ala Ser Ile Phe Ile Gly Gln Lys Asp Thr820 825 830Ser Asn Leu Pro Lys Glu Thr Val Lys Asn Ile Ser Thr Trp Gly Ala835 840 845Lys Pro Lys Leu Lys Asp Leu Ser Asn Tyr Ile Lys Tyr Thr Lys Asp850 855 860Lys Ser Thr Val Trp Val Ser Thr Ala Ile Asn Thr Glu Ala Gly Gly865 870 875 880Gln Ser Ser Gly Ala Pro Leu His Lys Ile Asp Met Asp Leu Tyr Glu885 890 895Phe Ala Ile Asp Gly Gln Lys Leu Asn Pro Leu Pro Glu Gly Arg Thr900 905 910
Lys Asn Met Val Pro Ser Leu Leu Leu Asp Thr Pro Gln Ile Glu Thr915 920 925Ser Ser Ile Ile Ala Leu Asn His Gly Pro Val Asn Asp Ala Glu Ile930 935 940Ser Phe Leu Thr Thr Ile Pro Leu Lys Ash Val Lys Pro His Lys Arg945 950 955 960<210>48<211>4521<212>DNA<213>伯氏致病杆菌(Xenorhabdus bovienii)<400>48atgaaacaag attcacagga catgacagta acacagctgt ccctgcccaa agggggcggt 60gcgatcagtg gcatgggtga cactatcagc aatgcagggc cggatgggat ggcttcgctt120tccgtgcctt tgcctatctc tgccggtcgg gggggcgcac cgaatttatc cctgaactac180agtagcggag caggaaacgg gtcatttggt attggctggc aatccagtac catggctatc240agccgtcgta ctcaacatgg cgtaccgcaa tatcacggcg aagatacttt tttatgtccg300atgggagaag tgatggcggt tgccgtcaat cagagcgggc aacccgatgt gcgtaaaacc360gataaactat taggcgggca actgcctgtt acttataccg ttacgcgtca tcagcccaga420aatattcagc acttcagcaa acttgaatac tggcagcccc caacggatgt ggaaaccacg480cctttttggt taatgtattc acccgatgga caaattcaca ttttcggaaa aactgagcag540gctcagatcg ctaacccggc agaggtttca cagattgccc aatggctttt ggaagaaacc600gtaacaccag cgggagaaca catttattac cagtatcggg cagaagacga tatcggttgt660gatgacagcg aaaaaaatgc ccaccctaat gccagtgctc aacgttattt gactcaggtg720
aactacggca atattacacc tgaatccagc ctgcttgtgc tgaagaatac gccaccggcg 780gataacgaat ggctattcca tttggttttt gattatggtg aacgagcgca ggaaataaac 840acggttcctc ctttcaaagc accttcaaac aactggaaaa tacggccaga ccgtttctcc 900cgctttgaat atggttttga ggtgcgaacc cgccgcctgt gtcaacaaat tctgatgttc 960catcgcctga aatcccttgc aggagaacag attgacggag aagaaatccc tgccttggtt1020gcccgtctgc ttctcagtta tgacctgaac gacagcgtga caacccttac cgccattcgg1080caaatggcgt atgaaactga cgcaacctta atcgctttac cgccactgga gtttgactat1140cagccctttg aggcaaaagt cacgcagaaa tggcaggaaa tgcctcaatt ggccggattg1200aatgcccaac aaccttacca actcgtcgat ctctatggtg aaggtatctc cggcatcttg1260tatcaggaca gacccggagc atggtggtat caggcaccga tccgtcagaa aaacgttgaa1320gatattaacg ctgtcaccta tagcccaata aaccccttac ctaagatccc cagccagcag1380gacagagcaa cgttgatgga tatcgacggt gatggacatc tggattgggt gatcgctggc1440gcaggtattc aggggcggta cagtatgcag ccgaatggag agtggacaca ctttattccc1500atttctgcac tgccaacaga atattttcat ccacaggcac aactggcgga tctggtgggg1560gccgggttat ctgatttagc gctgattggc cccagaagtg tgcgtttata tgccaacgac1620cgaggaaact ggaaagcggg tattaatgtt atgccacctg atggtgtgaa tttgccgata1680tttggtggtg atgccagcag tctggtcgca ttttctgaca tgttgggatc gggacagcag1740catttggtgg aaattgccgc tcagagcgtc aaatgctggc cgaatctagg acatggccgt1800tttggtgcgg ctattttgct gccggggttt agccagccga atggaacatt caatgctaac1860caagtttttc tggcagatat cgatggttcc ggcaccgccg acatcatcta tgcacacagt1920acgtatctgg atatttacct gaacgaaagc ggcaaccgtt tcagtgcacc cgttcggctt1980aatttgccgg aaggggtgat gtttgacaat acctgtcagt tacaggtgtc ggatattcaa2040
ggattgggcg ctgccagcat tgtactgacc gtacctcata tgacaccgcg ccattggcgt2100tatgatttta ctcacaataa accttggctg ctcaatgtca tcaacaacaa tcgtggcgca2160gaaaccacgt tgttttaccg tagttctgcc caattctggc tggatgaaaa aagtcagatc2220gaagagctgg gaaaatttgc agcgagttat ctgcctttcc ccatacattt gttgtggcgc2280aatgaggcgc tggatgaaat tactggtaat cgactgacta aggtcatgaa ttatgcccac2340ggtgcatggg atggcagaga gagagaattt tgcggatttg gccgtgtaac gcaaattgat2400accgacgaat ttgccaaggg aaccacagag aaagcgccgg atgaaaatat ctatccttcc2460cgtagcataa gctggtttgc cacgggttta ccagaagtgg attctcaact tccggcagaa2520tactggcgtg gtgacgatca ggcatttgcc ggctttacac cgcgcttcac tcgttatgaa2580aaaggtaatg cggggcaaga ggggcaggat accccgatta aagaaccgac cgaaacagaa2640gcgtattggc ttaaccgcgc catgaaaggc caattactgc gcagtgaagt ctatggtgac2700gacaaaacag aaaaagctaa aattccgtac accgtcacag aagctcgctg tcaggtcaga2760ttaattccca gcaatgacga agccgcgccg tcgtcttgga cgtcgatcat tgaaaaccgc2820agttatcact atgagcgtat cgtcgtcgat ccgagttgca aacaacaggt cgtgctcaag2880gcggatgaat atggcttccc actggcaaaa gtagatatcg cctatccacg gcgcaataaa2940ccggcacaga acccttatcc ggattcgtta ccggatactc tgttcgccga tagctatgac3000gaccagcaaa aacagttata tctgacaaaa cagcagcaga gctattacca cctgacccag3060caggatgatt gggttctggg tttgacggat agccgataca gcgaagttta tcattatgcg3120caaactgacg ctcaaagtga catccccaag gcagggctga tattggaaga cctgctgaaa3180gttgacggcc tgataggtaa agacaagact tttatctatt tagggcagca gcgagtggct3240tatgtgggag gagatgcaga aaaaccgaca cgtcaggtgc gggtggctta tacagaaacc3300gctgcttttg atgacaatgc gctgcacgcc tttgatggcg tgattgcccc tgatgaactg3360
acgcaacagt tgctggcggg tggatacctg ctcgtgccgc agatttctga tgtggcaggc3420agtagtgaaa aggtatgggt agctcggcag ggatacaccg aatacggcag tgctgctcaa3480ttctaccggc cactcatcca gcgcaaaagc ttgctgaccg gaaaatatac ccttagttgg3540gatacgcact attgtgtggt ggtaaaaacc gaagatggtg cgggaatgac cacgcaagcg3600aagtacgatt accgcttcct gcttccggcg caattgacag atatcaatga caaccagcac3660atcgtgacat ttaatgcatt ggggcaggtg acttccagcc gtttctgggg cacagaaaat3720ggcaaaataa gcggttactc gacgccggag agtaaaccgt tcacagtacc cgataccgtc3780gaaaaagccc ttgccttgca accgacgatc ccggtttcac agtgcaacat ttatgtgccg3840gatagttgga tgcggcttct gccccaacag tctctgactg gccagctaaa agagggggaa3900actttgtgga acgcattaca ccgggcgggt gtagtaacgg aagacggttt gatctgtgaa3960ctggcctatc gtcgttggat caaacgtcag gcaacgtctt caatgatggc cgtgacatta4020cagcaaatct tggctcagac tccacgacaa cctccgcatg ccatgacgat cacgacagat4080cgttatgaca gcgattctca gcagcaactt cggcagtcga tagtattgag tgatggtttt4140ggtcgcgtat tgcaaagcgc ccagcgtcat gaagcaggag aggcatggca gcgtgcagaa4200gatggttctt tggttgtcga taataccggt aaacccgttg ttgctaatac cacaacgcgc4260tgggcagtat ccggtcgcac agaatacgac ggcaaagggc aggcgatcag agcttacctg4320ccttattatc tcaatgattg gcgctatgtc agtgatgaca gcgcccggga tgacctgtac4380gccgataccc atttttacga tcctctgggg cgtgaatatc aggtaaaaac cgcgaaagga4440ttttggcgtg aaaacatgtt tatgccgtgg tttgtcgtca atgaagatga aaatgacaca4500gcagcacgtt taacatctta a 4521<210>49<211>1506<212>PRT
<213>伯氏致病杆菌<400>49Met Lys Gln Asp Ser Gln Asp Met Thr Val Thr Gln Leu Ser Leu Pro1 5 10 15Lys Gly Gly Gly Ala Ile Ser Gly Met Gly Asp Thr Ile Ser Asn Ala20 25 30Gly Pro Asp Gly Met Ala Ser Leu Ser Val Pro Leu Pro Ile Ser Ala35 40 45Gly Arg Gly Gly Ala Pro Asn Leu Ser Leu Asn Tyr Ser Ser Gly Ala50 55 60Gly Asn Gly Ser Phe Gly Ile Gly Trp Gln Ser Ser Thr Met Ala Ile65 70 75 80Ser Arg Arg Thr Gln His Gly Val Pro Gln Tyr His Gly Glu Asp Thr85 90 95Phe Leu Cys Pro Met Gly Glu Val Met Ala Val Ala Val Asn Gln Ser100 105 110Gly Gln Pro Asp Val Arg Lys Thr Asp Lys Leu Leu Gly Gly Gln Leu115 120 125Pro Val Thr Tyr Thr Val Thr Arg His Gln Pro Arg Asn Ile Gln His130 135 140Phe Ser Lys Leu Glu Tyr Trp Gln Pro Pro Thr Asp Val Glu Thr Thr145 150 155 160
Pro Phe Trp Leu Met Tyr Ser Pro Asp Gly Gln Ile His Ile Phe Gly165 170 175Lys Thr Glu Gln Ala Gln Ile Ala Asn Pro Ala Glu Val Ser Gln Ile180 185 190Ala Gln Trp Leu Leu Glu Glu Thr Val Thr Pro Ala Gly Glu His Ile195 200 205Tyr Tyr Gln Tyr Arg Ala Glu Asp Asp Ile Gly Cys Asp Asp Ser Glu210 215 220Lys Asn Ala His Pro Asn Ala Ser Ala Gln Arg Tyr Leu Thr Gln Val225 230 235 240Asn Tyr Gly Asn Ile Thr Pro Glu Ser Ser Leu Leu Val Leu Lys Asn245 250 255Thr Pro Pro Ala Asp Asn Glu Trp Leu Phe His Leu Val Phe Asp Tyr260 265 270Gly Glu Arg Ala Gln Glu Ile Asn Thr Val Pro Pro Phe Lys Ala Pro275 280 285Ser Asn Asn Trp Lys Ile Arg Pro Asp Arg Phe Ser Arg Phe Glu Tyr290 295 300Gly Phe Glu Val Arg Thr Arg Arg Leu Cys Gln Gln Ile Leu Met Phe305 310 315 320His Arg Leu Lys Ser Leu Ala Gly Glu Gln Ile Asp Gly Glu Glu Ile325 330 335
Pro Ala Leu Val Ala Arg Leu Leu Leu Ser Tyr Asp Leu Asn Asp Ser340 345 350Val Thr Thr Leu Thr Ala Ile Arg Gln Met Ala Tyr Glu Thr Asp Ala355 360 365Thr Leu Ile Ala Leu Pro Pro Leu Glu Phe Asp Tyr Gln Pro Phe Glu370 375 380Ala Lys Val Thr Gln Lys Trp Gln Glu Met Pro Gln Leu Ala Gly Leu385 390 395 400Asn Ala Gln Gln Pro Tyr Gln Leu Val Asp Leu Tyr Gly Glu Gly Ile405 410 415Ser Gly Ile Leu Tyr Gln Asp Arg Pro Gly Ala Trp Trp Tyr Gln Ala420 425 430Pro Ile Arg Gln Lys Asn Val Glu Asp Ile Asn Ala Val Thr Tyr Ser435 440 445Pro Ile Asn Pro Leu Pro Lys Ile Pro Ser Gln Gln Asp Arg Ala Thr450 455 460Leu Met Asp Ile Asp Gly Asp Gly His Leu Asp Trp Val Ile Ala Gly465 470 475 480Ala Gly Ile Gln Gly Arg Tyr Ser Met Gln Pro Asn Gly Glu Trp Thr485 490 495His Phe Ile Pro Ile Ser Ala Leu Pro Thr Glu Tyr Phe His Pro Gln500 505 510
Ala Gln Leu Ala Asp Leu Val Gly Ala Gly Leu Ser Asp Leu Ala Leu515 520 525Ile Gly Pro Arg Ser Val Arg Leu Tyr Ala Asn Asp Arg Gly Asn Trp530 535 540Lys Ala Gly Ile Asn Val Met Pro Pro Asp Gly Val Asn Leu Pro Ile545 550 555 560Phe Gly Gly Asp Ala Ser Ser Leu Val Ala Phe Ser Asp Met Leu Gly565 570 575Ser Gly Gln Gln His Leu Val Glu Ile Ala Ala Gln Ser Val Lys Cys580 585 590Trp Pro Asn Leu Gly His Gly Arg Phe Gly Ala Ala Ile Leu Leu Pro595 600 605Gly Phe Ser Gln Pro Asn Gly Thr Phe Asn Ala Asn Gln Val Phe Leu610 615 620Ala Asp Ile Asp Gly Ser Gly Thr Ala Asp Ile Ile Tyr Ala His Ser625 630 635 640Thr Tyr Leu Asp Ile Tyr Leu Asn Glu Ser Gly Asn Arg Phe Ser Ala645 650 655Pro Val Arg Leu Asn Leu Pro Glu Gly Val Met Phe Asp Asn Thr Cys660 665 670Gln Leu Gln Val Ser Asp Ile Gln Gly Leu Gly Ala Ala Ser Ile Val675 680 685
Leu Thr Val Pro His Met Thr Pro Arg His Trp Arg Tyr Asp Phe Thr690 695 700His Asn Lys Pro Trp Leu Leu Asn Val Ile Asn Asn Asn Arg Gly Ala705 710 715 720Glu Thr Thr Leu Phe Tyr Arg Ser Ser Ala Gln Phe Trp Leu Asp Glu725 730 735Lys Ser Gln Ile Glu Glu Leu Gly Lys Phe Ala Ala Ser Tyr Leu Pro740 745 750Phe Pro Ile His Leu Leu Trp Arg Asn Glu Ala Leu Asp Glu Ile Thr755 760 765Gly Asn Arg Leu Thr Lys Val Met Asn Tyr Ala His Gly Ala Trp Asp770 775 780Gly Arg Glu Arg Glu Phe Cys Gly Phe Gly Arg Val Thr Gln Ile Asp785 790 795 800Thr Asp Glu Phe Ala Lys Gly Thr Thr Glu Lys Ala Pro Asp Glu Asn805 810 815Ile Tyr Pro Ser Arg Ser Ile Ser Trp Phe Ala Thr Gly Leu Pro Glu820 825 830Val Asp Ser Gln Leu Pro Ala Glu Tyr Trp Arg Gly Asp Asp Gln Ala835 840 845Phe Ala Gly Phe Thr Pro Arg Phe Thr Arg Tyr Glu Lys Gly Asn Ala850 855 860
Gly Gln Glu Gly Gln Asp Thr Pro Ile Lys Glu Pro Thr Glu Thr Glu865 870 875 880Ala Tyr Trp Leu Asn Arg Ala Met Lys Gly Gln Leu Leu Arg Ser Glu885 890 895Val Tyr Gly Asp Asp Lys Thr Glu Lys Ala Lys Ile Pro Tyr Thr Val900 905 910Thr Glu Ala Arg Cys Gln Val Arg Leu Ile Pro Ser Asn Asp Glu Ala915 920 925Ala Pro Ser Ser Trp Thr Ser Ile Ile Glu Asn Arg Ser Tyr His Tyr930 935 940Glu Arg Ile Val Val Asp Pro Ser Cys Lys Gln Gln Val Val Leu Lys945 950 955 960Ala Asp Glu Tyr Gly Phe Pro Leu Ala Lys Val Asp Ile Ala Tyr Pro965 970 975Arg Arg Asn Lys Pro Ala Gln Asn Pro Tyr Pro Asp Ser Leu Pro Asp980 985 990Thr Leu Phe Ala Asp Ser Tyr Asp Asp Gln Gln Lys Gln Leu Tyr Leu995 1000 1005Thr Lys Gln Gln Gln Ser Tyr Tyr His Leu Thr Gln Gln Asp Asp1010 1015 1020Trp Val Leu Gly Leu Thr Asp Ser Arg Tyr Ser Glu Val Tyr His1025 1030 1035
Tyr Ala Gln Thr Asp Ala Gln Ser Asp Ile Pro Lys Ala Gly Leu1040 1045 1050Ile Leu Glu Asp Leu Leu Lys Val Asp Gly Leu Ile Gly Lys Asp1055 1060 1065Lys Thr Phe Ile Tyr Leu Gly Gln Gln Arg Val Ala Tyr Val Gly1070 1075 1080Gly Asp Ala Glu Lys Pro Thr Arg Gln Val Arg Val Ala Tyr Thr1085 1090 1095Glu Thr Ala Ala Phe Asp Asp Asn Ala Leu His Ala Phe Asp Gly1100 1105 1110Val Ile Ala Pro Asp Glu Leu Thr Gln Gln Leu Leu Ala Gly Gly1115 1120 1125Tyr Leu Leu Val Pro Gln Ile Ser Asp Val Ala Gly Ser Ser Glu1130 1135 1140Lys Val Trp Val Ala Arg Gln Gly Tyr Thr Glu Tyr Gly Ser Ala1145 1150 1155Ala Gln Phe Tyr Arg Pro Leu Ile Gln Arg Lys Ser Leu Leu Thr1160 1165 1170Gly Lys Tyr Thr Leu Ser Trp Asp Thr His Tyr Cys Val Val Val1175 1180 1185Lys Thr Glu Asp Gly Ala Gly Met Thr Thr Gln Ala Lys Tyr Asp1190 1195 1200
Tyr Arg Phe Leu Leu Pro Ala Gln Leu Thr Asp Ile Asn Asp Asn1205 1210 1215Gln His Ile Val Thr Phe Asn Ala Leu Gly Gln Val Thr Ser Ser1220 1225 1230Arg Phe Trp Gly Thr Glu Asn Gly Lys Ile Ser Gly Tyr Ser Thr1235 1240 1245Pro Glu Ser Lys Pro Phe Thr Val Pro Asp Thr Val Glu Lys Ala1250 1255 1260Leu Ala Leu Gln Pro Thr Ile Pro Val Ser Gln Cys Asn Ile Tyr1265 1270 1275Val Pro Asp Ser Trp Met Arg Leu Leu Pro Gln Gln Ser Leu Thr1280 1285 1290Gly Gln Leu Lys Glu Gly Glu Thr Leu Trp Asn Ala Leu His Arg1295 1300 1305Ala Gly Val Val Thr Glu Asp Gly Leu Ile Cys Glu Leu Ala Tyr1310 1315 1320Arg Arg Trp Ile Lys Arg Gln Ala Thr Ser Ser Met Met Ala Val1325 1330 1335Thr Leu Gln Gln Ile Leu Ala Gln Thr Pro Arg Gln Pro Pro His1340 1345 1350Ala Met Thr Ile Thr Thr Asp Arg Tyr Asp Ser Asp Ser Gln Gln1355 1360 1365
Gln Leu Arg Gln Ser Ile Val Leu Ser Asp Gly Phe Gly Arg Val1370 1375 1380Leu Gln Ser Ala Gln Arg His Glu Ala Gly Glu Ala Trp Gln Arg1385 1390 1395Ala Glu Asp Gly Ser Leu Val Val Asp Asn Thr Gly Lys Pro Val1400 1405 1410Val Ala Asn Thr Thr Thr Arg Trp Ala Val Ser Gly Arg Thr Glu1415 1420 1425Tyr Asp Gly Lys Gly Gln Ala Ile Arg Ala Tyr Leu Pro Tyr Tyr1430 1435 1440Leu Asn Asp Trp Arg Tyr Val Ser Asp Asp Ser Ala Arg Asp Asp1445 1450 1455Leu Tyr Ala Asp Thr His Phe Tyr Asp Pro Leu Gly Arg Glu Tyr1460 1465 1470Gln Val Lys Thr Ala Lys Gly Phe Trp Arg Glu Asn Met Phe Met1475 1480 1485Pro Trp Phe Val Val Asn Glu Asp Glu Asn Asp Thr Ala Ala Arg1490 1495 1500Leu Thr Ser1505<210>50<211>2889<212>DNA<213>伯氏致病杆菌
<400>50atgaatgttt ttaatccaac tttatatgcc ggtacaccga ctgtcaccgt catggacaat 60cgagggctgt cagtgcggga tattgcttat caccgtacaa cagcaggaga gcaggctgac 120actcgcatca cccgccatca atacagtccc cataattttt taatcgagag cattgatcca 180cgcctttttg atttgcaatc tcagagcacc ataaaaccta atttcaccta ctgtcctgcc 240ttgaagggtg atgtcctacg gacagagagt gtggatgccg gacaaactgt cattttgagt 300gacatcgaag gtcgtccgtt actgaatatc agtgcgatgg gtgtcgtcaa acactggcaa 360tatgaagaga gtacattgcc ggggcgcttg ctcgctgtca gtgaacggaa gaatgaggct 420tcaacacccc aaattattga acggtttatt tggtcgggaa atagcccatc agaaaaagat 480cacaatttgg cgggaaaata tcttcgtcat tatgataccg ccggattaaa ccagcttaat 540gctgtgtctc tgaccagcgt ggatctctca caatcccgtc agttattgca ggatgatgtc 600acagcagatt ggagcggaag tgacgaatcc cagtggaaga cgcgactgag taacgacata 660ttcacaaccg aaatcaccgc tgatgcggtt ggcaatttct tgactcagaa tgatgccaaa 720agcaaccagc aacgattgtc ctatgatgtg gcagggcagt taaaggcaag ctggctgacg 780ataaaaggcc agaatgagca ggtgatagtt aactccctga cttactccgc cgcagggcag 840aaactgcgtg aagagcaggg taacggcgtt gtcactgaat actcctatga agcacaaacc 900tggcgtttga taggtgtaac ggcttaccgt cagtcagata aaaaaagatt gcaggatctt 960gtctataact atgatccggt cggtaatctc ctgaatattc gcaataatgc agaggcaacc1020cgtttctggc gtaatcagat agtagaacca gagaaccact atgcttatga ctcgctttat1080caactcatca gtgctagtgg tcgagaaatc gccagtatcg gtcagcaggg cagccggctg1140cctgtaccga ttattcctct tcctgccaat gacgatgttt atactcgcta cacccgcaca1200tatcactatg atcgcggtgg aaatctctgc cagatccggc attgcgctcc tgctacagat1260
aataagtaca ccacaaagat caccgtatcg aatcgtagta atcgtgcagt atgggatacc1320ttgaccacag atcccgccaa agtggatacc ctgtttgatc atggagggca tcaacttcaa1380ctccagtcag gccagacttt atgttggaac tatcggggtg aactacagca aataacaaag1440atacagcgtg acgaaaaacc cgcagataaa gagcggtatc gctatggtgt tggggctgcg1500cgggtcgtga aaatcagcac acagcaggcg gggggaagca gccatgtgca gcgtgttgtt1560tatctgccgg ggttggaact acgcacaact cagcatgatg cgacattaat cgaagactta1620caggtgatta tcatgggtga agcaggacgt gctcaggtac gcgtacttca ttgggaaata1680ccaccaccgg ataatcttaa caatgactca ctgcgttaca gctacgatag tttgatgggt1740tccagtcagc ttgaattgga tggagcaggg cagattatta cgcaggaaga atactacccc1800tatggaggta cagcaatatg ggcggcaaga aaccagaccg aagccaatta caaaaccatt1860cgctactccg gcaaagagcg tgatgcgacg gggctttatt actacgggca ccgttattat1920cagccgtggc tagggcgctg gttgagcgca gatcccgccg gaaccgtgga cggactgaat1980ctatatcgaa tggtgaggaa taacccgatt acttaccggg atgcagatgg gcttgcgccg2040ataggcgata agatcagcga agggatttat gagcctgagt tgcgagttgg tcttgaacga2100gatgacccaa atgtcagaga ttatgaccgg gtttatcctg atacggccaa gacagagatg2160atcgaagcaa ctgcgaccac aattgctccc agtcaaatgt tatcggcgca tgcttttgca2220tctgtaccta tattgacaga tttgtttaat cctcaaacag caaggctttc tcaaaagaca2280acggatattg tattaaacac acaaggtgga ggcgatttaa tctttactgg catgaatatt2340aaaggtaagg gaaaagaatt taatgcatta aaaatcgttg atacttatgg cggagaaatg2400cctgatagca aaaccgctat ttcagcatat tggcttccgc aaggtgggta tactgatatt2460ccgatacatc cgactggaat acaaaagtat ttgtttacgc ctgcgtttag tggttgcact2520ctggcagtag ataagcttaa cgaaaataca ttacgggcgt atcacgtcga aggaagtaag2580
gaagatgctc aatataataa tttagcagtt gcagcgcacg gagagggttt ggtcatggct 2640atggaatttc ctgactatgg atttcataca gacaaaacag ggcaaagact aaggaacaca 2700cagggatttg cgtttatgtc ctacaatcaa tcccagaaaa aatgggaaat tcattatcaa 2760aggcaagcat tgacatcaaa caccggtatc atgaatgtta gtgctaaaaa caagattcga 2820ttgaatgccc ccagtcatgt aaaaaatagc tcaatcaaag gaactgaaat aatgacgaca 2880catttttaa 2889<210>51<211>962<212>PRT<213>伯氏致病杆菌<400>51Met Asn Val Phe Asn Pro Thr Leu Tyr Ala Gly Thr Pro Thr Val Thr1 5 10 15Val Met Asp Asn Arg Gly Leu Ser Val Arg Asp Ile Ala Tyr His Arg20 25 30Thr Thr Ala Gly Glu Gln Ala Asp Thr Arg Ile Thr Arg His Gln Tyr35 40 45Ser Pro His Asn Phe Leu Ile Glu Ser Ile Asp Pro Arg Leu Phe Asp50 55 60Leu Gln Ser Gln Ser Thr Ile Lys Pro Ash Phe Thr Tyr Cys Pro Ala65 70 75 80Leu Lys Gly Asp Val Leu Arg Thr Glu Ser Val Asp Ala Gly Gln Thr85 90 95
Val Ile Leu Ser Asp Ile Glu Gly Arg Pro Leu Leu Asn Ile Ser Ala100 105 110Met Gly Val Val Lys His Trp Gln Tyr Glu Glu Ser Thr Leu Pro Gly115 120 125Arg Leu Leu Ala Val Ser Glu Arg Lys Asn Glu Ala Ser Thr Pro Gln130 135 140Ile Ile Glu Arg Phe Ile Trp Ser Gly Asn Ser Pro Ser Glu Lys Asp145 150 155 160His Asn Leu Ala Gly Lys Tyr Leu Arg His Tyr Asp Thr Ala Gly Leu165 170 175Asn Gln Leu Asn Ala Val Ser Leu Thr Ser Val Asp Leu Ser Gln Ser180 185 190Arg Gln Leu Leu Gln Asp Asp Val Thr Ala Asp Trp Ser Gly Ser Asp195 200 205Glu Ser Gln Trp Lys Thr Arg Leu Ser Asn Asp Ile Phe Thr Thr Glu210 215 220Ile Thr Ala Asp Ala Val Gly Asn Phe Leu Thr Gln Asn Asp Ala Lys225 230 235 240Ser Asn Gln Gln Arg Leu Ser Tyr Asp Val Ala Gly Gln Leu Lys Ala245 250 255Ser Trp Leu Thr Ile Lys Gly Gln Asn Glu Gln Val Ile Val Asn Ser260 265 270
Leu Thr Tyr Ser Ala Ala Gly Gln Lys Leu Arg Glu Glu Gln Gly Asn275 280 285Gly Val Val Thr Glu Tyr Ser Tyr Glu Ala Gln Thr Trp Arg Leu Ile290 295 300Gly Val Thr Ala Tyr Arg Gln Ser Asp Lys Lys Arg Leu Gln Asp Leu305 310 315 320Val Tyr Asn Tyr Asp Pro Val Gly Asn Leu Leu Asn Ile Arg Asn Asn325 330 335Ala Glu Ala Thr Arg Phe Trp Arg Asn Gln Ile Val Glu Pro Glu Asn340 345 350His Tyr Ala Tyr Asp Ser Leu Tyr Gln Leu Ile Ser Ala Ser Gly Arg355 360 365Glu Ile Ala Ser Ile Gly Gln Gln Gly Ser Arg Leu Pro Val Pro Ile370 375 380Ile Pro Leu Pro Ala Asn Asp Asp Val Tyr Thr Arg Tyr Thr Arg Thr385 390 395 400Tyr His Tyr Asp Arg Gly Gly Asn Leu Cys Gln Ile Arg His Cys Ala405 410 415Pro Ala Thr Asp Asn Lys Tyr Thr Thr Lys Ile Thr Val Ser Asn Arg420 425 430Ser Asn Arg Ala Val Trp Asp Thr Leu Thr Thr Asp Pro Ala Lys Val435 440 445
Asp Thr Leu Phe Asp His Gly Gly His Gln Leu Gln Leu Gln Ser Gly450 455 460Gln Thr Leu Cys Trp Asn Tyr Arg Gly Glu Leu Gln Gln Ile Thr Lys465 470 475 480Ile Gln Arg Asp Glu Lys Pro Ala Asp Lys Glu Arg Tyr Arg Tyr Gly485 490 495Val Gly Ala Ala Arg Val Val Lys Ile Ser Thr Gln Gln Ala Gly Gly500 505 510Ser Ser His Val Gln Arg Val Val Tyr Leu Pro Gly Leu Glu Leu Arg515 520 525Thr Thr Gln His Asp Ala Thr Leu Ile Glu Asp Leu Gln Val Ile Ile530 535 540Met Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp Glu Ile545 550 555 560Pro Pro Pro Asp Asn Leu Asn Asn Asp Ser Leu Arg Tyr Ser Tyr Asp565 570 575Ser Leu Met Gly Ser Ser Gln Leu Glu Leu Asp Gly Ala Gly Gln Ile580 585 590Ile Thr Gln Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr Ala Ile Trp Ala595 600 605Ala Arg Asn Gln Thr Glu Ala Asn Tyr Lys Thr Ile Arg Tyr Ser Gly610 615 620
Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly His Arg Tyr Tyr625 630 635 640Gln Pro Trp Leu Gly Arg Trp Leu Ser Ala Asp Pro Ala Gly Thr Val645 650 655Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Ile Thr Tyr660 665 670Arg Asp Ala Asp Gly Leu Ala Pro Ile Gly Asp Lys Ile Ser Glu Gly675 680 685Ile Tyr Glu Pro Glu Leu Arg Val Gly Leu Glu Arg Asp Asp Pro Asn690 695 700Val Arg Asp Tyr Asp Arg Val Tyr Pro Asp Thr Ala Lys Thr Glu Met705 710 715 720Ile Glu Ala Thr Ala Thr Thr Ile Ala Pro Ser Gln Met Leu Ser Ala725 730 735His Ala Phe Ala Ser Val Pro Ile Leu Thr Asp Leu Phe Asn Pro Gln740 745 750Thr Ala Arg Leu Ser Gln Lys Thr Thr Asp Ile Val Leu Asn Thr Gln755 760 765Gly Gly Gly Asp Leu Ile Phe Thr Gly Met Asn Ile Lys Gly Lys Gly770 775 780Lys Glu Phe Asn Ala Leu Lys Ile Val Asp Thr Tyr Gly Gly Glu Met785 790 795 800
Pro Asp Ser Lys Thr Ala Ile Ser Ala Tyr Trp Leu Pro Gln Gly Gly805 810 815Tyr Thr Asp Ile Pro Ile His Pro Thr Gly Ile Gln Lys Tyr Leu Phe820 825 830Thr Pro Ala Phe Ser Gly Cys Thr Leu Ala Val Asp Lys Leu Asn Glu835 840 845Asn Thr Leu Arg Ala Tyr His Val Glu Gly Ser Lys Glu Asp Ala Gln850 855 860Tyr Asn Asn Leu Ala Val Ala Ala His Gly Glu Gly Leu Val Met Ala865 870 875 880Met Glu Phe Pro Asp Tyr Gly Phe His Thr Asp Lys Thr Gly Gln Arg885 890 895Leu Arg Asn Thr Gln Gly Phe Ala Phe Met Ser Tyr Asn Gln Ser Gln900 905 910Lys Lys Trp Glu Ile His Tyr Gln Arg Gln Ala Leu Thr Ser Asn Thr915 920 925Gly Ile Met Asn Val Ser Ala Lys Asn Lys Ile Arg Leu Asn Ala Pro930 935 940Ser His Val Lys Asn Ser Ser Ile Lys Gly Thr Glu Ile Met Thr Thr945 950 955 960His Phe
<210>52<211>4595<212>DNA<213>伯氏致病杆菌<400>52tctagaacta gtgtcgacta aagaagaagg agatatacca tgaaacaaga cagccaggac 60atgacagtaa cacagctgtc cctgcccaaa gggggcggtg cgatcagtgg catgggtgac 120actatcagca atgcagggcc ggatgggatg gcttcgcttt ccgtgccttt gcctatctct 180gccggtcggg ggggcgcacc gaatttatcc ctgaactaca gtagcggagc aggaaacggg 240tcatttggta ttggctggca atccagtacc atggctatca gccgtcgtac tcaacatggc 300gtaccgcaat atcacggcga agatactttt ttatgtccga tgggagaagt gatggcggtt 360gccgtcaatc agagcgggca acccgatgtg cgtaaaaccg ataaactatt aggcgggcaa 420ctgcctgtta cttataccgt tacgcgtcat cagcccagaa atattcagca cttcagcaaa 480cttgaatact ggcagccccc aacggatgtg gaaaccacgc ctttttggtt aatgtattca 540cccgatggac aaattcacat tttcggaaaa actgagcagg ctcagatcgc taacccggca 600gaggtttcac agattgccca atggcttttg gaagaaaccg taacaccagc gggagaacac 660atttattacc agtatcgggc agaagacgat atcggttgtg atgacagcga aaaaaatgcc 720caccctaatg ccagtgctca acgttatttg actcaggtga actacggcaa tattacacct 780gaatccagcc tgcttgtgct gaagaatacg ccaccggcgg ataacgaatg gctattccat 840ttggtttttg attatggtga acgagcgcag gaaataaaca cggttcctcc tttcaaagca 900ccttcaaaca actggaaaat acggccagac cgtttctccc gctttgaata tggttttgag 960gtgcgaaccc gccgcctgtg tcaacaaatt ctgatgttcc atcgcctgaa atcccttgca1020ggagaacaga ttgacggaga agaaatccct gccttggttg cccgtctgct tctcagttat1080gacctgaacg acagcgtgac aacccttacc gccattcggc aaatggcgta tgaaactgac1140
gcaaccttaa tcgctttacc gccactggag tttgactatc agccctttga ggcaaaagtc1200acgcagaaat ggcaggaaat gcctcaattg gccggattga atgcccaaca accttaccaa1260ctcgtcgatc tctatggtga aggtatctcc ggcatcttgt atcaggacag acccggagca1320tggtggtatc aggcaccgat ccgtcagaaa aacgttgaag atattaacgc tgtcacctat1380agcccaataa accccttacc taagatcccc agccagcagg acagagcaac gttgatggat1440atcgacggtg atggacatct ggattgggtg atcgctggcg caggtattca ggggcggtac1500agtatgcagc cgaatggaga gtggacacac tttattccca tttctgcact gccaacagaa1560tattttcatc cacaggcaca actggcggat ctggtggggg ccgggttatc tgatttagcg1620ctgattggcc ccagaagtgt gcgtttatat gccaacgacc gaggaaactg gaaagcgggt1680attaatgtta tgccacctga tggtgtgaat ttgccgatat ttggtggtga tgccagcagt1740ctggtcgcat tttctgacat gttgggatcg ggacagcagc atttggtgga aattgccgct1800cagagcgtca aatgctggcc gaatctagga catggccgtt ttggtgcggc tattttgctg1860ccggggttta gccagccgaa tggaacattc aatgctaacc aagtttttct ggcagatatc1920gatggttccg gcaccgccga catcatctat gcacacagta cgtatctgga tatttacctg1980aacgaaagcg gcaaccgttt cagtgcaccc gttcggctta atttgccgga aggggtgatg2040tttgacaata cctgtcagtt acaggtgtcg gatattcaag gattgggcgc tgccagcatt2100gtactgaccg tacctcatat gacaccgcgc cattggcgtt atgattttac tcacaataaa2160ccttggctgc tcaatgtcat caacaacaat cgtggcgcag aaaccacgtt gttttaccgt2220agttctgccc aattctggct ggatgaaaaa agtcagatcg aagagctggg aaaatttgca2280gcgagttatc tgcctttccc catacatttg ttgtggcgca atgaggcgct ggatgaaatt2340actggtaatc gactgactaa ggtcatgaat tatgcccacg gtgcatggga tggcagagag2400agagaatttt gcggatttgg ccgtgtaacg caaattgata ccgacgaatt tgccaaggga2460
accacagaga aagcgccgga tgaaaatatc tatccttccc gtagcataag ctggtttgcc2520acgggtttac cagaagtgga ttctcaactt ccggcagaat actggcgtgg tgacgatcag2580gcatttgccg gctttacacc gcgcttcact cgttatgaaa aaggtaatgc ggggcaagag2640gggcaggata ccccgattaa agaaccgacc gaaacagaag cgtattggct taaccgcgcc2700atgaaaggcc aattactgcg cagtgaagtc tatggtgacg acaaaacaga aaaagctaaa2760attccgtaca ccgtcacaga agctcgctgt caggtcagat taattcccag caatgacgaa2820gccgcgccgt cgtcttggac gtcgatcatt gaaaaccgca gttatcacta tgagcgtatc2880gtcgtcgatc cgagttgcaa acaacaggtc gtgctcaagg cggatgaata tggcttccca2940ctggcaaaag tagatatcgc ctatccacgg cgcaataaac cggcacagaa cccttatccg3000gattcgttac cggatactct gttcgccgat agctatgacg accagcaaaa acagttatat3060ctgacaaaac agcagcagag ctattaccac ctgacccagc aggatgattg ggttctgggt3120ttgacggata gccgatacag cgaagtttat cattatgcgc aaactgacgc tcaaagtgac3180atccccaagg cagggctgat attggaagac ctgctgaaag ttgacggcct gataggtaaa3240gacaagactt ttatctattt agggcagcag cgagtggctt atgtgggagg agatgcagaa3300aaaccgacac gtcaggtgcg ggtggcttat acagaaaccg ctgcttttga tgacaatgcg3360ctgcacgcct ttgatggcgt gattgcccct gatgaactga cgcaacagtt gctggcgggt3420ggatacctgc tcgtgccgca gatttctgat gtggcaggca gtagtgaaaa ggtatgggta3480gctcggcagg gatacaccga atacggcagt gctgctcaat tctaccggcc actcatccag3540cgcaaaagct tgctgaccgg aaaatatacc cttagttggg atacgcacta ttgtgtggtg3600gtaaaaaccg aagatggtgc gggaatgacc acgcaagcga agtacgatta ccgcttcctg3660cttccggcgc aattgacaga tatcaatgac aaccagcaca tcgtgacatt taatgcattg3720gggcaggtga cttccagccg tttctggggc acagaaaatg gcaaaataag cggttactcg3780
acgccggaga gtaaaccgtt cacagtaccc gataccgtcg aaaaagccct tgccttgcaa 3840ccgacgatcc cggtttcaca gtgcaacatt tatgtgccgg atagttggat gcggcttctg 3900ccccaacagt ctctgactgg ccagctaaaa gagggggaaa ctttgtggaa cgcattacac 3960cgggcgggtg tagtaacgga agacggtttg atctgtgaac tggcctatcg tcgttggatc 4020aaacgtcagg caacgtcttc aatgatggcc gtgacattac agcaaatctt ggctcagact 4080ccacgacaac ctccgcatgc catgacgatc acgacagatc gttatgacag cgattctcag 4140cagcaacttc ggcagtcgat agtattgagt gatggttttg gtcgcgtatt gcaaagcgcc 4200cagcgtcatg aagcaggaga ggcatggcag cgtgcagaag atggttcttt ggttgtcgat 4260aataccggta aacccgttgt tgctaatacc acaacgcgct gggcagtatc cggtcgcaca 4320gaatacgacg gcaaagggca ggcgatcaga gcttacctgc cttattatct caatgattgg 4380cgctatgtca gtgatgacag cgcccgggat gacctgtacg ccgataccca tttttacgat 4440cctctggggc gtgaatatca ggtaaaaacc gcgaaaggat tttggcgtga aaacatgttt 4500atgccgtggt ttgtcgtcaa tgaagatgaa aatgacacag cagcacgttt aacatcttaa 4560ttaatgcggc cgcaggcctc tgtaagactc tcgag 4595<210>53<211>2947<212>DNA<213>伯氏致病杆菌<400>53tctagaacta gtaggcctta aagaagagag agatatacca tgaatgtttt taatccaact60ttatatgccg gtacaccgac tgtcaccgtc atggacaatc gagggctgtc agtgcgggat 120attgcttatc accgtacaac agcaggagag caggctgaca ctcgcatcac ccgccatcaa 180tacagtcccc ataatttttt aatcgagagc attgatccac gcctttttga tttgcaatct 240cagagcacca taaaacctaa tttcacctac tgtcctgcct tgaagggtga tgtcctacgg 300
acagagagtg tggatgccgg acaaactgtc attttgagtg acatcgaagg tcgtccgtta 360ctgaatatca gtgcgatggg tgtcgtcaaa cactggcaat atgaagagag tacattgccg 420gggcgcttgc tcgctgtcag tgaacggaag aatgaggctt caacacccca aattattgaa 480cggtttattt ggtcgggaaa tagcccatca gaaaaagatc acaatttggc gggaaaatat 540cttcgtcatt atgataccgc cggattaaac cagcttaatg ctgtgtctct gaccagcgtg 600gatctctcac aatcccgtca gttattgcag gatgatgtca cagcagattg gagcggaagt 660gacgaatccc agtggaagac gcgactgagt aacgacatat tcacaaccga aatcaccgct 720gatgcggttg gcaatttctt gactcagaat gatgccaaaa gcaaccagca acgattgtcc 780tatgatgtgg cagggcagtt aaaggcaagc tggctgacga taaaaggcca gaatgagcag 840gtgatagtta actccctgac ttactccgcc gcagggcaga aactgcgtga agagcagggt 900aacggcgttg tcactgaata ctcctatgaa gcacaaacct ggcgtttgat aggtgtaacg 960gcttaccgtc agtcagataa aaaaagattg caggatcttg tctataacta tgatccggtc1020ggtaatctcc tgaatattcg caataatgca gaggcaaccc gtttctggcg taatcagata1080gtagaaccag agaaccacta tgcttatgac tcgctttatc aactcatcag tgctagtggt1140cgagaaatcg ccagtatcgg tcagcagggc agccggctgc ctgtaccgat tattcctctt1200cctgccaatg acgatgttta tactcgctac acccgcacat atcactatga tcgcggtgga1260aatctctgcc agatccggca ttgcgctcct gctacagata ataagtacac cacaaagatc1320accgtatcga atcgtagtaa tcgtgcagta tgggatacct tgaccacaga tcccgccaaa1380gtggataccc tgtttgatca tggagggcat caacttcaac tccagtcagg ccagacttta1440tgttggaact atcggggtga actacagcaa ataacaaaga tacagcgtga cgaaaaaccc1500gcagataaag agcggtatcg ctatggtgtt ggggctgcgc gggtcgtgaa aatcagcaca1560cagcaggcgg ggggaagcag ccatgtgcag cgtgttgttt atctgccggg gttggaacta1620
cgcacaactc agcatgatgc gacattaatc gaagacttac aggtgattat catgggtgaa1680gcaggacgtg ctcaggtacg cgtacttcat tgggaaatac caccaccgga taatcttaac1740aatgactcac tgcgttacag ctacgatagt ttgatgggtt ccagtcagct tgaattggat1800ggagcagggc agattattac gcaggaagaa tactacccct atggaggtac agcaatatgg1860gcggcaagaa accagaccga agccaattac aaaaccattc gctactccgg caaagagcgt1920gatgcgacgg ggctttatta ctacgggcac cgttattatc agccgtggct agggcgctgg1980ttgagcgcag atcccgccgg aaccgtggac ggactgaatc tatatcgaat ggtgaggaat2040aacccgatta cttaccggga tgcagatggg cttgcgccga taggcgataa gatcagcgaa2100gggatttatg agcctgagtt gcgagttggt cttgaacgag atgacccaaa tgtcagagat2160tatgaccggg tttatcctga tacggccaag acagagatga tcgaagcaac tgcgaccaca2220attgctccca gtcaaatgtt atcggcgcat gcttttgcat ctgtacctat attgacagat2280ttgtttaatc ctcaaacagc aaggctttct caaaagacaa cggatattgt attaaacaca2340caaggtggag gcgatttaat ctttactggc atgaatatta aaggtaaggg aaaagaattt2400aatgcattaa aaatcgttga tacttatggc ggagaaatgc ctgatagcaa aaccgctatt2460tcagcatatt ggcttccgca aggtgggtat actgatattc cgatacatcc gactggaata2520caaaagtatt tgtttacgcc tgcgtttagt ggttgcactc tggcagtaga taagcttaac2580gaaaatacat tacgggcgta tcacgtcgaa ggaagtaagg aagatgctca atataataat2640ttagcagttg cagcgcacgg agagggtttg gtcatggcta tggaatttcc tgactatgga2700tttcatacag acaaaacagg gcaaagacta aggaacacac agggatttgc gtttatgtcc2760tacaatcaat cccagaaaaa atgggaaatt cattatcaaa ggcaagcatt gacatcaaac2820accggtatca tgaatgttag tgctaaaaac aagattcgat tgaatgcccc cagtcatgta2880aaaaatagct caatcaaagg aactgaaata atgacgacac atttttaatt aatgcggccg2940
cctcgag2947<210>54<211>7508<212>DNA<213>伯氏致病杆菌<400>54tctagaacta gtgtcgacta aagaagaagg agatatacca tgaaacaaga cagccaggac60atgacagtaa cacagctgtc cctgcccaaa gggggcggtg cgatcagtgg catgggtgac 120actatcagca atgcagggcc ggatgggatg gcttcgcttt ccgtgccttt gcctatctct 180gccggtcggg ggggcgcacc gaatttatcc ctgaactaca gtagcggagc aggaaacggg 240tcatttggta ttggctggca atccagtacc atggctatca gccgtcgtac tcaacatggc 300gtaccgcaat atcacggcga agatactttt ttatgtccga tgggagaagt gatggcggtt 360gccgtcaatc agagcgggca acccgatgtg cgtaaaaccg ataaactatt aggcgggcaa 420ctgcctgtta cttataccgt tacgcgtcat cagcccagaa atattcagca cttcagcaaa 480cttgaatact ggcagccccc aacggatgtg gaaaccacgc ctttttggtt aatgtattca 540cccgatggac aaattcacat tttcggaaaa actgagcagg ctcagatcgc taacccggca 600gaggtttcac agattgccca atggcttttg gaagaaaccg taacaccagc gggagaacac 660atttattacc agtatcgggc agaagacgat atcggttgtg atgacagcga aaaaaatgcc 720caccctaatg ccagtgctca acgttatttg actcaggtga actacggcaa tattacacct 780gaatccagcc tgcttgtgct gaagaatacg ccaccggcgg ataacgaatg gctattccat 840ttggtttttg attatggtga acgagcgcag gaaataaaca cggttcctcc tttcaaagca 900ccttcaaaca actggaaaat acggccagac cgtttctccc gctttgaata tggttttgag 960gtgcgaaccc gccgcctgtg tcaacaaatt ctgatgttcc atcgcctgaa atcccttgca 1020
ggagaacaga ttgacggaga agaaatccct gccttggttg cccgtctgct tctcagttat1080gacctgaacg acagcgtgac aacccttacc gccattcggc aaatggcgta tgaaactgac1140gcaaccttaa tcgctttacc gccactggag tttgactatc agccctttga ggcaaaagtc1200acgcagaaat ggcaggaaat gcctcaattg gccggattga atgcccaaca accttaccaa1260ctcgtcgatc tctatggtga aggtatctcc ggcatcttgt atcaggacag acccggagca1320tggtggtatc aggcaccgat ccgtcagaaa aacgttgaag atattaacgc tgtcacctat1380agcccaataa accccttacc taagatcccc agccagcagg acagagcaac gttgatggat1440atcgacggtg atggacatct ggattgggtg atcgctggcg caggtattca ggggcggtac1500agtatgcagc cgaatggaga gtggacacac tttattccca tttctgcact gccaacagaa1560tattttcatc cacaggcaca actggcggat ctggtggggg ccgggttatc tgatttagcg1620ctgattggcc ccagaagtgt gcgtttatat gccaacgacc gaggaaactg gaaagcgggt1680attaatgtta tgccacctga tggtgtgaat ttgccgatat ttggtggtga tgccagcagt1740ctggtcgcat tttctgacat gttgggatcg ggacagcagc atttggtgga aattgccgct1800cagagcgtca aatgctggcc gaatctagga catggccgtt ttggtgcggc tattttgctg1860ccggggttta gccagccgaa tggaacattc aatgctaacc aagtttttct ggcagatatc1920gatggttccg gcaccgccga catcatctat gcacacagta cgtatctgga tatttacctg1980aacgaaagcg gcaaccgttt cagtgcaccc gttcggctta atttgccgga aggggtgatg2040tttgacaata cctgtcagtt acaggtgtcg gatattcaag gattgggcgc tgccagcatt2100gtactgaccg tacctcatat gacaccgcgc cattggcgtt atgattttac tcacaataaa2160ccttggctgc tcaatgtcat caacaacaat cgtggcgcag aaaccacgtt gttttaccgt2220agttctgccc aattctggct ggatgaaaaa agtcagatcg aagagctggg aaaatttgca2280gcgagttatc tgcctttccc catacatttg ttgtggcgca atgaggcgct ggatgaaatt2340
actggtaatc gactgactaa ggtcatgaat tatgcccacg gtgcatggga tggcagagag2400agagaatttt gcggatttgg ccgtgtaacg caaattgata ccgacgaatt tgccaaggga2460accacagaga aagcgccgga tgaaaatatc tatccttccc gtagcataag ctggtttgcc2520acgggtttac cagaagtgga ttctcaactt ccggcagaat actggcgtgg tgacgatcag2580gcatttgccg gctttacacc gcgcttcact cgttatgaaa aaggtaatgc ggggcaagag2640gggcaggata ccccgattaa agaaccgacc gaaacagaag cgtattggct taaccgcgcc2700atgaaaggcc aattactgcg cagtgaagtc tatggtgacg acaaaacaga aaaagctaaa2760attccgtaca ccgtcacaga agctcgctgt caggtcagat taattcccag caatgacgaa2820gccgcgccgt cgtcttggac gtcgatcatt gaaaaccgca gttatcacta tgagcgtatc2880gtcgtcgatc cgagttgcaa acaacaggtc gtgctcaagg cggatgaata tggcttccca2940ctggcaaaag tagatatcgc ctatccacgg cgcaataaac cggcacagaa cccttatccg3000gattcgttac cggatactct gttcgccgat agctatgacg accagcaaaa acagttatat3060ctgacaaaac agcagcagag ctattaccac ctgacccagc aggatgattg ggttctgggt3120ttgacggata gccgatacag cgaagtttat cattatgcgc aaactgacgc tcaaagtgac3180atccccaagg cagggctgat attggaagac ctgctgaaag ttgacggcct gataggtaaa3240gacaagactt ttatctattt agggcagcag cgagtggctt atgtgggagg agatgcagaa3300aaaccgacac gtcaggtgcg ggtggcttat acagaaaccg ctgcttttga tgacaatgcg3360ctgcacgcct ttgatggcgt gattgcccct gatgaactga cgcaacagtt gctggcgggt3420ggatacctgc tcgtgccgca gatttctgat gtggcaggca gtagtgaaaa ggtatgggta3480gctcggcagg gatacaccga atacggcagt gctgctcaat tctaccggcc actcatccag3540cgcaaaagct tgctgaccgg aaaatatacc cttagttggg atacgcacta ttgtgtggtg3600gtaaaaaccg aagatggtgc gggaatgacc acgcaagcga agtacgatta ccgcttcctg3660
cttccggcgc aattgacaga tatcaatgac aaccagcaca tcgtgacatt taatgcattg3720gggcaggtga cttccagccg tttctggggc acagaaaatg gcaaaataag cggttactcg3780acgccggaga gtaaaccgtt cacagtaccc gataccgtcg aaaaagccct tgccttgcaa3840ccgacgatcc cggtttcaca gtgcaacatt tatgtgccgg atagttggat gcggcttctg3900ccccaacagt ctctgactgg ccagctaaaa gagggggaaa ctttgtggaa cgcattacac3960cgggcgggtg tagtaacgga agacggtttg atctgtgaac tggcctatcg tcgttggatc4020aaacgtcagg caacgtcttc aatgatggcc gtgacattac agcaaatctt ggctcagact4080ccacgacaac ctccgcatgc catgacgatc acgacagatc gttatgacag cgattctcag4140cagcaacttc ggcagtcgat agtattgagt gatggttttg gtcgcgtatt gcaaagcgcc4200cagcgtcatg aagcaggaga ggcatggcag cgtgcagaag atggttcttt ggttgtcgat4260aataccggta aacccgttgt tgctaatacc acaacgcgct gggcagtatc cggtcgcaca4320gaatacgacg gcaaagggca ggcgatcaga gcttacctgc cttattatct caatgattgg4380cgctatgtca gtgatgacag cgcccgggat gacctgtacg ccgataccca tttttacgat4440cctctggggc gtgaatatca ggtaaaaacc gcgaaaggat tttggcgtga aaacatgttt4500atgccgtggt ttgtcgtcaa tgaagatgaa aatgacacag cagcacgttt aacatcttaa4560ttaatgcggc cgcaggcctt aaagaagaga gagatatacc atgaatgttt ttaatccaac4620tttatatgcc ggtacaccga ctgtcaccgt catggacaat cgagggctgt cagtgcggga4680tattgcttat caccgtacaa cagcaggaga gcaggctgac actcgcatca cccgccatca4740atacagtccc cataattttt taatcgagag cattgatcca cgcctttttg atttgcaatc4800tcagagcacc ataaaaccta atttcaccta ctgtcctgcc ttgaagggtg atgtcctacg4860gacagagagt gtggatgccg gacaaactgt cattttgagt gacatcgaag gtcgtccgtt4920actgaatatc agtgcgatgg gtgtcgtcaa acactggcaa tatgaagaga gtacattgcc4980
ggggcgcttg ctcgctgtca gtgaacggaa gaatgaggct tcaacacccc aaattattga5040acggtttatt tggtcgggaa atagcccatc agaaaaagat cacaatttgg cgggaaaata5100tcttcgtcat tatgataccg ccggattaaa ccagcttaat gctgtgtctc tgaccagcgt5160ggatctctca caatcccgtc agttattgca ggatgatgtc acagcagatt ggagcggaag5220tgacgaatcc cagtggaaga cgcgactgag taacgacata ttcacaaccg aaatcaccgc5280tgatgcggtt ggcaatttct tgactcagaa tgatgccaaa agcaaccagc aacgattgtc5340ctatgatgtg gcagggcagt taaaggcaag ctggctgacg ataaaaggcc agaatgagca5400ggtgatagtt aactccctga cttactccgc cgcagggcag aaactgcgtg aagagcaggg5460taacggcgtt gtcactgaat actcctatga agcacaaacc tggcgtttga taggtgtaac5520ggcttaccgt cagtcagata aaaaaagatt gcaggatctt gtctataact atgatccggt5580cggtaatctc ctgaatattc gcaataatgc agaggcaacc cgtttctggc gtaatcagat5640agtagaacca gagaaccact atgcttatga ctcgctttat caactcatca gtgctagtgg5700tcgagaaatc gccagtatcg gtcagcaggg cagccggctg cctgtaccga ttattcctct5760tcctgccaat gacgatgttt atactcgcta cacccgcaca tatcactatg atcgcggtgg5820aaatctctgc cagatccggc attgcgctcc tgctacagat aataagtaca ccacaaagat5880caccgtatcg aatcgtagta atcgtgcagt atgggatacc ttgaccacag atcccgccaa5940agtggatacc ctgtttgatc atggagggca tcaacttcaa ctccagtcag gccagacttt6000atgttggaac tatcggggtg aactacagca aataacaaag atacagcgtg acgaaaaacc6060cgcagataaa gagcggtatc gctatggtgt tggggctgcg cgggtcgtga aaatcagcac6120acagcaggcg gggggaagca gccatgtgca gcgtgttgtt tatctgccgg ggttggaact6180acgcacaact cagcatgatg cgacattaat cgaagactta caggtgatta tcatgggtga6240agcaggacgt gctcaggtac gcgtacttca ttgggaaata ccaccaccgg ataatcttaa6300
caatgactca ctgcgttaca gctacgatag tttgatgggt tccagtcagc ttgaattgga6360tggagcaggg cagattatta cgcaggaaga atactacccc tatggaggta cagcaatatg6420ggcggcaaga aaccagaccg aagccaatta caaaaccatt cgctactccg gcaaagagcg6480tgatgcgacg gggctttatt actacgggca ccgttattat cagccgtggc tagggcgctg6540gttgagcgca gatcccgccg gaaccgtgga cggactgaat ctatatcgaa tggtgaggaa6600taacccgatt acttaccggg atgcagatgg gcttgcgccg ataggcgata agatcagcga6660agggatttat gagcctgagt tgcgagttgg tcttgaacga gatgacccaa atgtcagaga6720ttatgaccgg gtttatcctg atacggccaa gacagagatg atcgaagcaa ctgcgaccac6780aattgctccc agtcaaatgt tatcggcgca tgcttttgca tctgtaccta tattgacaga6840tttgtttaat cctcaaacag caaggctttc tcaaaagaca acggatattg tattaaacac6900acaaggtgga ggcgatttaa tctttactgg catgaatatt aaaggtaagg gaaaagaatt6960taatgcatta aaaatcgttg atacttatgg cggagaaatg cctgatagca aaaccgctat7020ttcagcatat tggcttccgc aaggtgggta tactgatatt ccgatacatc cgactggaat7080acaaaagtat ttgtttacgc ctgcgtttag tggttgcact ctggcagtag ataagcttaa7140cgaaaataca ttacgggcgt atcacgtcga aggaagtaag gaagatgctc aatataataa7200tttagcagtt gcagcgcacg gagagggttt ggtcatggct atggaatttc ctgactatgg7260atttcataca gacaaaacag ggcaaagact aaggaacaca cagggatttg cgtttatgtc7320ctacaatcaa tcccagaaaa aatgggaaat tcattatcaa aggcaagcat tgacatcaaa7380caccggtatc atgaatgtta gtgctaaaaa caagattcga ttgaatgccc ccagtcatgt7440aaaaaatagc tcaatcaaag gaactgaaat aatgacgaca catttttaat taatgcggcc7500gcctcgag 7508<210>55
<211>2862<212>DNA<213>类芽孢杆菌DAS1529株<400>55atgaaaatga taccgtggac tcaccattat ttgcttcacc gcctgcgcgg tgagatggag 60gttaaaccta tgaacacaac gtccatatat aggggcacgc ctacgatttc agttgtggat120aaccggaact tggagattcg cattcttcag tataaccgta tcgcggctga agatccggca180gatgagtgta tcctgcggaa cacgtatacg ccgttaagct atcttggcag cagcatggat240ccccgtttgt tctcgcaata tcaggatgat cgcggaacac cgccgaatat acgaaccatg300gcttccctga gaggcgaagc gctgtgttcg gaaagtgtgg atgccggccg caaggcggag360ctttttgata tcgaggggcg gcccgtctgg cttatcgatg ccaacggcac agagacgact420ctcgaatatg atgtcttagg caggccaaca gccgtattcg agcaacagga aggtacggac480tccccccagt gcagggagcg gtttatttat ggtgagaagg aggcggatgc ccaggccaac540aatttgcgcg gacaactggt tcgccactac gataccgcgg gccggataca gaccgacagc600atctccttgg ctggactgcc gttgcgccaa agccgtcaac tgctgaaaaa ttgggatgaa660cctggcgact ggagtatgga tgaggaaagc gcctgggcct cgttgctggc tgccgaagct720tatgatacga gctggcggta tgacgcgcag gacagggtgc tcgcccaaac cgacgccaaa780gggaatctcc agcaactgac ttacaatgac gccggccagc cgcaggcggt cagcctcaag840ctgcaaggcc aagcggagca acggatttgg aaccggatcg agtacaacgc ggcgggtcaa900gtggatctcg ccgaagccgg gaatggaatc gtaacggaat atacttacga ggaaagcacg960cagcggttaa tccgaaaaaa agattcccgc ggactgtcct ccggggaaag agaagtgctg 1020caggattatc gttatgaata tgatccggta ggcaatatcc tttctattta caatgaagcg 1080gagccggttc gttatttccg caatcaggcc gttgctccga aaaggcaata tgcctacgat 1140gccttgtatc agcttgtatc tagttcgggg cgggaatccg acgcgcttcg gcagcagacg 1200
tcgcttcctc ccttgatcac gcctatccct ctggacgata gccaatacgt caattacgct1260gaaaaataca gctatgatca ggcgggcaat ttaatcaagc ttagccataa cggggcaagt1320caatatacaa cgaatgtgta tgtggacaaa agctcaaacc gggggatttg gcggcaaggg1380gaagacatcc cggatatcgc ggcttccttt gacagagcag gcaatcaaca agctttattc1440ccggggagac cgttggaatg ggatacacgc aatcaattaa gccgtgtcca tatggtcgtg1500cgcgaaggcg gagacaacga ctgggaaggc tatctctatg acagctcggg aatgcgtatc1560gtaaaacgat ctacccgcaa aacacagaca acgacgcaaa cggatacgac cctctatttg1620ccgggcctgg agctgcgaat ccgccagacc ggggaccggg tcacggaagc attgcaggtc1680attaccgtgg atgagggagc gggacaagtg agggtgctgc actgggagga tggaaccgag1740ccgggcggca tcgccaatga tcagtaccgg tacagcctga acgatcatct tacctcctct1800ttattggaag ttgacgggca aggtcagatc attagtaagg aagaatttta tccctatggc1860ggcacagccc tgtggacagc ccggtcagag gtagaggcaa gctacaagac catccgctat1920tcaggcaaag agcgggatgc cacaggcctg tattattacg gacaccgcta ctatatgcca1980tggttgggtc gctggctgaa tccggacccg gccggaatgg tagatggact aaacctgtac2040cgtatggtca ggaacaatcc tataggactg atggatccga atgggaatgc gccaatcaac2100gtggcggatt atagcttcgt gcatggtgat ttagtttatg gtcttagtaa ggaaagagga2160agatatctaa agctatttaa tccaaacttt aatatggaaa aatcagactc tcctgctatg2220gttatagatc aatataataa taatgttgca ttgagtataa ctaaccaata taaagtagaa2280gaattgatga aatttcaaaa agacccacaa aaagccgcac ggaaaataaa ggttccagaa2340gggaatcgtt tatcgaggaa cgaaaattat cctttgtggc acgattatat taacattgga2400gaagctaaag ctgcatttaa ggcctctcat attttccaag aagtgaaggg gaattatggg2460aaagattatt atcataaatt attattagac agaatgatag aatcgccgtt gctgtggaaa2520
cgaggcagca aactcgggct agaaatcgcc gctaccaatc agagaacaaa aatacacttt 2580gttcttgaca atttaaatat cgagcaggtg gttacgaaag agggtagcgg cggtcagtca 2640atcacagctt cggagctccg ttatatttat cgaaatcgcg aaagattgaa cgggcgtgtc 2700attttctata gaaataatga aaggctagat caggctccat ggcaagaaaa tccggactta 2760tggagcaaat atcaaccggg tcttagacaa agcagcagtt caagagtcaa agaacgaggg 2820attgggaact ttttccgccg gttttcaatg aagagaaagt aa 2862<210>56<211>4458<212>DNA<213>发光光杆状菌W14株<220>
<221>外显子<222>(1)..(4458)<400>56atg cag gat tca cca gaa gta tcg att aca acg ctg tca ctt ccc aaa 48Met Gln Asp Ser Pro Glu Val Ser Ile Thr Thr Leu Ser Leu Pro Lys1 5 10 15ggt ggc ggt gct atc aat ggc atg gga gaa gca ctg aat gct gcc ggc 96Gly Gly Gly Ala Ile Asn Gly Met Gly Glu Ala Leu Asn Ala Ala Gly20 25 30cct gat gga atg gcc tcc cta tct ctg cca tta ccc ctt tcg acc ggc144Pro Asp Gly Met Ala Ser Leu Ser Leu Pro Leu Pro Leu Ser Thr Gly35 40 45aga ggg acg gct cct gga tta tcg ctg att tac agc aac agt gca ggt192Arg Gly Thr Ala Pro Gly Leu Ser Leu Ile Tyr Ser Asn Ser Ala Gly50 55 60aat ggg cct ttc ggc atc ggc tgg caa tgc ggt gtt atg tcc att agc240Asn Gly Pro Phe Gly Ile Gly Trp Gln Cys Gly Val Met Ser Ile Ser65 70 75 80
cga cgc acc caa cat ggc att cca caa tac ggt aat gac gac acg ttc288Arg Arg Thr Gln His Gly Ile Pro Gln Tyr Gly Asn Asp Asp Thr Phe85 90 95cta tcc cca caa ggc gag gtc atg aat atc gcc ctg aat gac caa ggg336Leu Ser Pro Gln Gly Glu Val Met Asn Ile Ala Leu Asn Asp Gln Gly100 105 110caa cct gat atc cgt caa gac gtt aaa acg ctg caa ggc gtt acc ttg384Gln Pro Asp Ile Arg Gln Asp Val Lys Thr Leu Gln Gly Val Thr Leu115 120 125cca att tcc tat acc gtg acc cgc tat caa gcc cgc cag atc ctg gat432Pro Ile Ser Tyr Thr Val Thr Arg Tyr Gln Ala Arg Gln Ile Leu Asp130 135 140ttc agt aaa atc gaa tac tgg caa cct gcc tcc ggt caa gaa gga cgc480Phe Ser Lys Ile Glu Tyr Trp Gln Pro Ala Ser Gly Gln Glu Gly Arg145 150 155 160gct ttc tgg ctg ata tcg tca ccg gac ggc caa cta cac atc tta ggg528Ala Phe Trp Leu Ile Ser Ser Pro Asp Gly Gln Leu His Ile Leu Gly165 170 175aaa acc gcg cag gct tgt ctg gca aat ccg caa aat gac caa caa atc576Lys Thr Ala Gln Ala Cys Leu Ala Asn Pro Gln Asn Asp Gln Gln Ile180 185 190gcc cag tgg ttg ctg gaa gaa act gtg acg cca gcc ggt gaa cat gtc624Ala Gln Trp Leu Leu Glu Glu Thr Val Thr Pro Ala Gly Glu His Val195 200 205agc tat caa tat cga gcc gaa gat gaa gcc cat tgt gac gac aat gaa672Ser Tyr Gln Tyr Arg Ala Glu Asp Glu Ala His Cys Asp Asp Asn Glu210 215 220aaa acc gct cat ccc aat gtt acc gca cag cgc tat ctg gta cag gtg720Lys Thr Ala His Pro Asn Val Thr Ala Gln Arg Tyr Leu Val Gln Val225 230 235 240aac tac ggc aac atc aaa cca caa gcc agc ctg ttc gta ctg gat aac768Asn Tyr Gly Asn Ile Lys Pro Gln Ala Ser Leu Phe Val Leu Asp Asn245 250 255
gca cct ccc gca ccg gaa gag tgg ctg ttt cat ctg gtc ttt gac cac 816Ala Pro Pro Ala Pro Glu Glu Trp Leu Phe His Leu Val Phe Asp His260 265 270ggt gag cgc gat acc tca ctt cat acc gtg cca aca tgg gat gca ggt 864Gly Glu Arg Asp Thr Ser Leu His Thr Val Pro Thr Trp Asp Ala Gly275 280 285aca gcg caa tgg tct gta cgc ccg gat atc ttc tct cgc tat gaa tat 912Thr Ala Gln Trp Ser Val Arg Pro Asp Ile Phe Ser Arg Tyr Glu Tyr290 295 300ggt ttt gaa gtg cgt act cgc cgc tta tgt caa caa gtg ctg atg ttt 960Gly Phe Glu Val Arg Thr Arg Arg Leu Cys Gln Gln Val Leu Met Phe305 310 315 320cac cgc acc gcg ctc atg gcc gga gaa gcc agt acc aat gac gcc ccg1008His Arg Thr Ala Leu Met Ala Gly Glu Ala Ser Thr Asn Asp Ala Pro325 330 335gaa ctg gtt gga cgc tta ata ctg gaa tat gac aaa aac gcc agc gtc1056Glu Leu Val Gly Arg Leu Ile Leu Glu Tyr Asp Lys Asn Ala Ser Val340 345 350acc acg ttg att acc atc cgt caa tta agc cat gaa tcg gac ggc agc1104Thr Thr Leu Ile Thr Ile Arg Gln Leu Ser His Glu Ser Asp Gly Ser355 360 365cca gtc acc cag cca cca cta gaa cta gcc tgg caa cgg ttt gat ctg1152Pro Val Thr Gln Pro Pro Leu Glu Leu Ala Trp Gln Arg Phe Asp Leu370 375 380gag aaa atg ccg aca tgg caa cgc ttt gac gca cta gat aat ttt aac1200Glu Lys Met Pro Thr Trp Gln Arg Phe Asp Ala Leu Asp Asn Phe Asn385 390 395 400tcg cag caa cgt tat caa ctg gtt gat ctg cgg gga gaa ggg ttg cca1248Ser Gln Gln Arg Tyr Gln Leu Val Asp Leu Arg Gly Glu Gly Leu Pro405 410 415ggt atg ctg tat caa gat cga ggc gct tgg tgg tat aaa gct ccg caa1296Gly Met Leu Tyr Gln Asp Arg Gly Ala Trp Trp Tyr Lys Ala Pro Gln420 425 430
cgt cag gaa gac gga gac agc aat gcc gtc act tac gac aaa atc gcc1344Arg Gln Glu Asp Gly Asp Ser Asn Ala Val Thr Tyr Asp Lys Ile Ala435 440 445cca ctg cct acc cta ccc aat ttg cag gat aat gcc tca ttg atg gat1392Pro Leu Pro Thr Leu Pro Asn Leu Gln Asp Asn Ala Ser Leu Met Asp450 455 460atc aac gga gac ggc caa ctg gat tgg gtt gtt acc gcc tcc ggt att1440Ile Asn Gly Asp Gly Gln Leu Asp Trp Val Val Thr Ala Ser Gly Ile465 470 475 480cgc gga tac cat agt cag caa ccc gat gga aag tgg acg cac ttt acg1488Arg Gly Tyr His Ser Gln Gln Pro Asp Gly Lys Trp Thr His Phe Thr485 490 495cca atc aat gcc ttg ccc gtg gaa tat ttt cat cca agc atc cag ttc1536Pro Ile Asn Ala Leu Pro Val Glu Tyr Phe His Pro Ser Ile Gln Phe500 505 510gct gac ctt acc ggg gca ggc tta tct gat tta gtg ttg atc ggg ccg1584Ala Asp Leu Thr G1y Ala Gly Leu Ser Asp Leu Val Leu Ile Gly Pro515 520 525aaa agc gtg cgt cta tat gcc aac cag cga aac ggc tgg cgt aaa gga1632Lys Ser Val Arg Leu Tyr Ala Asn Gln Arg Asn Gly Trp Arg Lys Gly530 535 540gaa gat gtc ccc caa tcc aca ggt atc acc ctg cct gtc aca ggg acc1680Glu Asp Val Pro Gln Ser Thr Gly Ile Thr Leu Pro Val Thr Gly Thr545 550 555 560gat gcc cgc aaa ctg gtg gct ttc agt gat atg ctc ggt tcc ggt caa1728Asp Ala Arg Lys Leu Val Ala Phe Ser Asp Met Leu Gly Ser Gly Gln565 570 575caa cat ctg gtg gaa atc aag gct aat cgc gtc acc tgt tgg ccg aat1776Gln His Leu Val Glu Ile Lys Ala Asn Arg Val Thr Cys Trp Pro Asn580 585 590cta ggg cat ggc cgt ttc ggt caa cca cta act ctg tca gga ttt agc1824Leu G1y His Gly Arg Phe Gly Gln Pro Leu Thr Leu Ser Gly Phe Ser595 600 605
cag ccc gaa aat agc ttc aat ccc gaa cgg ctg ttt ctg gcg gat atc1872Gln Pro Glu Asn Ser Phe Asn Pro Glu Arg Leu Phe Leu Ala Asp Ile610 615 620gac ggc tcc ggc acc acc gac ctt atc tat gcg caa tcc ggc tct ttg1920Asp Gly Ser Gly Thr Thr Asp Leu Ile Tyr Ala Gln Ser Gly Ser Leu625 630 635 640ctc att tat ctc aac caa agt ggt aat cag ttt gat gcc ccg ttg aca1968Leu Ile Tyr Leu Asn Gln Ser Gly Asn Gln Phe Asp Ala Pro Leu Thr645 650 655tta gcg ttg cca gaa ggc gta caa ttt gac aac act tgc caa ctt caa2016Leu Ala Leu Pro Glu Gly Val Gln Phe Asp Asn Thr Cys Gln Leu Gln660 665 670gtc gcc gat att cag gga tta ggg ata gcc agc ttg att ctg act gtg2064Val Ala Asp Ile Gln Gly Leu Gly Ile Ala Ser Leu Ile Leu Thr Val675 680 685cca cat atc gcg cca cat cac tgg cgt tgt gac ctg tca ctg acc aaa2112Pro His Ile Ala Pro His His Trp Arg Cys Asp Leu Ser Leu Thr Lys690 695 700ccc tgg ttg ttg aat gta atg aac aat aac cgg ggc gca cat cac acg2160Pro Trp Leu Leu Asn Val Met Asn Asn Asn Arg Gly Ala His His Thr705 710 715 720cta cat tat cgt agt tcc gcg caa ttc tgg ttg gat gaa aaa tta cag2208Leu His Tyr Arg Ser Ser Ala Gln Phe Trp Leu Asp Glu Lys Leu Gln725 730 735ctc acc aaa gca ggc aaa tct ccg gct tgt tat ctg ccg ttt cca atg2256Leu Thr Lys Ala Gly Lys Ser Pro Ala Cys Tyr Leu Pro Phe Pro Met740 745 750cat ttg cta tgg tat acc gaa att cag gat gaa atc agc ggc aac cgg2304His Leu Leu Trp Tyr Thr Glu Ile Gln Asp Glu Ile Ser Gly Asn Arg755 760 765ctc acc agt gaa gtc aac tac agc cac ggc gtc tgg gat ggt aaa gag2352Leu Thr Ser Glu Val Asn Tyr Ser His Gly Val Trp Asp Gly Lys Glu770 775 780
cgg gaa ttc aga gga ttt ggc tgc atc aaa cag aca gat acc aca acg2400Arg Glu Phe Arg Gly Phe Gly Cys Ile Lys Gln Thr Asp Thr Thr Thr785 790 795 800ttt tct cac ggc acc gcc ccc gaa cag gcg gca ccg tcg ctg agt att2448Phe Ser His Gly Thr Ala Pro Glu Gln Ala Ala Pro Ser Leu Ser Ile805 810 815agc tgg ttt gcc acc ggc atg gat gaa gta gac agc caa tta gct acg2496Ser Trp Phe Ala Thr Gly Met Asp Glu Val Asp Ser Gln Leu Ala Thr820 825 830gaa tat tgg cag gca gac acg caa gct tat agc gga ttt gaa acc cgt2544Glu Tyr Trp Gln Ala Asp Thr Gln Ala Tyr Ser Gly Phe Glu Thr Arg835 840 845tat acc gtc tgg gat cac acc aac cag aca gac caa gca ttt acc ccc2592Tyr Thr Val Trp Asp His Thr Asn Gln Thr Asp Gln Ala Phe Thr Pro850 855 860aat gag aca caa cgt aac tgg ctg acg cga gcg ctt aaa ggc caa ctg2640Asn Glu Thr Gln Arg Asn Trp Leu Thr Arg Ala Leu Lys Gly Gln Leu865 870 875 880cta cgc act gag ctc tac ggt ctg gac gga aca gat aag caa aca gtg2688Leu Arg Thr Glu Leu Tyr Gly Leu Asp Gly Thr Asp Lys Gln Thr Val885 890 895cct tat acc gtc agt gaa tcg cgc tat cag gta cgc tct att ccc gta2736Pro Tyr Thr Val Ser Glu Ser Arg Tyr Gln Val Arg SerIle Pro Val900 905 910aat aaa gaa act gaa tta tct gcc tgg gtg act gct att gaa aat cgc2784Asn Lys Glu Thr Glu Leu Ser Ala Trp Val Thr Ala Ile Glu Asn Arg915 920 925agc tac cac tat gaa cgt atc atc act gac cca cag ttc agc cag agt2832Ser Tyr His Tyr Glu Arg Ile Ile Thr Asp Pro Gln Phe Ser Gln Ser930 935 940atc aag ttg caa cac gat atc ttt ggt caa tca ctg caa agt gtc gat2880Ile Lys Leu Gln His Asp Ile Phe Gly Gln Ser Leu Gln Ser Val Asp945 950 955 960
att gcc tgg ccg cgc cgc gaa aaa cca gca gtg aat ccc tac ccg cct2928Ile Ala Trp Pro Arg Arg Glu Lys Pro Ala Val Asn Pro Tyr Pro Pro965 970 975acc ctg ccg gaa acg cta ttt gac agc agc tat gat gat caa caa caa2976Thr Leu Pro Glu Thr Leu Phe Asp Ser Ser Tyr Asp Asp Gln Gln Gln980 985 990cta tta cgt ctg gtg aga caa aaa aat agc tgg cat cac ctg act gat3024Leu Leu Arg Leu Val Arg Gln Lys Asn Ser Trp His His Leu Thr Asp995 10001005ggg gaa aac tgg cga tta ggt tta ccg aat gca caa cgc cgt gat 3069Gly Glu Asn Trp Arg Leu Gly Leu Pro Asn Ala Gln Arg Arg Asp1010 1015 1020gtt tat act tat gac cgg agc aaa att cca acc gaa ggg att tcc 3114Val Tyr Thr Tyr Asp Arg Ser Lys Ile Pro Thr Glu Gly Ile Ser1025 1030 1035ctt gaa atc ttg ctg aaa gat gat ggc ctg cta gca gat gaa aaa 3159Leu Glu Ile Leu Leu Lys Asp Asp Gly Leu Leu Ala Asp Glu Lys1040 1045 1050gcg gcc gtt tat ctg gga caa caa cag acg ttt tac acc gcc ggt 3204Ala Ala Val Tyr Leu Gly Gln Gln Gln Thr Phe Tyr Thr Ala Gly1055 1060 1065caa gcg gaa gtc act cta gaa aaa ccc acg tta caa gca ctg gtc 3249Gln Ala Glu Val Thr Leu Glu Lys Pro Thr Leu Gln Ala Leu Val1070 1075 1080gcg ttc caa gaa acc gcc atg atg gac gat acc tca tta cag gcg 3294Ala Phe Gln Glu Thr Ala Met Met Asp Asp Thr Ser Leu Gln Ala1085 1090 1095tat gaa ggc gtg att gaa gag caa gag ttg aat acc gcg ctg aca 3339Tyr Glu Gly Val Ile Glu Glu Gln Glu Leu Asn Thr Ala Leu Thr1100 1105 11l0cag gcc ggt tat cag caa gtc gcg cgg ttg ttt aat acc aga tca 3384Gln Ala Gly Tyr Gln Gln Val Ala Arg Leu Phe Asn Thr Arg Ser1115 1120 1125
gaa agc ccg gta tgg gcg gca cgg caa ggt tat acc gat tac ggt3429Glu Ser Pro Val Trp Ala Ala Arg Gln Gly Tyr Thr Asp Tyr Gly1130 1135 1140gac gcc gca cag ttc tgg cgg cct cag gct cag cgt aac tcg ttg3474Asp Ala Ala Gln Phe Trp Arg Pro Gln Ala Gln Arg Asn Ser Leu1145 1150 1155ctg aca ggg aaa acc aca ctg acc tgg gat acc cat cat tgt gta3519Leu Thr Gly Lys Thr Thr Leu Thr Trp Asp Thr His His Cys Val1160 1165 1170ata ata cag act caa gat gcc gct gga tta acg acg caa gcc cat3564Ile Ile Gln Thr Gln Asp Ala Ala Gly Leu Thr Thr Gln Ala His1175 1180 1185tac gat tat cgt ttc ctt aca ccg gta caa ctg aca gat att aat3609Tyr Asp Tyr Arg Phe Leu Thr Pro Val Gln Leu Thr Asp Ile Asn1190 1195 1200gat aat caa cat att gtg act ctg gac gcg cta ggt cgc gta acc3654Asp Asn Gln His Ile Val Thr Leu Asp Ala Leu Gly Arg Val Thr1205 1210 1215acc agc cgg ttc tgg ggc aca gag gca gga caa gcc gca ggc tat3699Thr Ser Arg Phe Trp Gly Thr Glu Ala Gly Gln Ala Ala Gly Tyr1220 1225 1230tcc aac cag ccc ttc aca cca ccg gac tcc gta gat aaa gcg ctg3744Ser Asn Gln Pro Phe Thr Pro Pro Asp Ser Val Asp Lys Ala Leu1235 1240 1245gca tta acc ggc gca ctc cct gtt gcc caa tgt tta gtc tat gcc3789Ala Leu Thr Gly Ala Leu Pro Val Ala Gln Cys Leu Val Tyr Ala1250 1255 1260gtt gat agc tgg atg ccg tcg tta tct ttg tct cag ctt tct cag3834Val Asp Ser Trp Met Pro Ser Leu Ser Leu Ser Gln Leu Ser Gln1265 1270 1275tca caa gaa gag gca gaa gcg cta tgg gcg caa ctg cgt gcc gct3879Ser Gln Glu Glu Ala Glu Ala Leu Trp Ala Gln Leu Arg Ala Ala1280 1285 1290
cat atg att acc gaa gat ggg aaa gtg tgt gcg tta agc ggg aaa3924His Met Ile Thr Glu Asp Gly Lys Val Cys Ala Leu Ser Gly Lys1295 1300 1305cga gga aca agc cat cag aac ctg acg att caa ctt att tcg cta3969Arg Gly Thr Ser His Gln Asn Leu Thr Ile Gln Leu Ile Ser Leu1310 1315 1320ttg gca agt att ccc cgt tta ccg cca cat gta ctg ggg atc acc4014Leu Ala Ser Ile Pro Arg Leu Pro Pro His Val Leu Gly Ile Thr1325 1330 1335act gat cgc tat gat agc gat ccg caa cag cag cac caa cag acg4059Thr Asp Arg Tyr Asp Ser Asp Pro Gln Gln Gln His Gln Gln Thr1340 1345 1350gtg agc ttt agt gac ggt ttt ggc cgg tta ctc cag agt tca gct4104Val Ser Phe Ser Asp Gly Phe Gly Arg Leu Leu Gln Ser Ser Ala1355 1360 1365cgt cat gag tca ggt gat gcc tgg caa cgt aaa gag gat ggc ggg4149Arg His Glu Ser Gly Asp Ala Trp Gln Arg Lys Glu Asp Gly Gly1370 1375 1380ctg gtc gtg gat gca aat ggc gtt ctg gtc agt gcc cct aca gac4194Leu Val Val Asp Ala Asn Gly Val Leu Val Ser Ala Pro Thr Asp1385 1390 1395acc cga tgg gcc gtt tcc ggt cgc aca gaa tat gac gac aaa ggc4239Thr Arg Trp Ala Val Ser Gly Arg Thr Glu Tyr Asp Asp Lys Gly1400 1405 1410caa cct gtg cgt act tat caa ccc tat ttt cta aat gac tgg cgt4284Gln Pro Val Arg Thr Tyr Gln Pro Tyr Phe Leu Asn Asp Trp Arg1415 1420 1425tac gtt agt gat gac agc gca cga gat gac ctg ttt gcc gat acc4329Tyr Val Ser Asp Asp Ser Ala Arg Asp Asp Leu Phe Ala Asp Thr1430 1435 1440cac ctt tat gat cca ttg gga cgg gaa tac aaa gtc atc act gct4374His Leu Tyr Asp Pro Leu Gly Arg Glu Tyr Lys Val Ile Thr Ala1445 1450 1455
aag aaa tat ttg cga gaa aag ctg tac acc ccg tgg ttt att gtc 4419Lys Lys Tyr Leu Arg Glu Lys Leu Tyr Thr Pro Trp Phe Ile Val1460 1465 1470agt gag gat gaa aac gat aca gca tca aga acc cca tag 4458Ser Glu Asp Glu Asn Asp Thr Ala Ser Arg Thr Pro1475 1480 1485<210>57<211>2817<212>DNA<213>发光光杆状菌W14株<220>
<221>外显子<222>(1)..(2817)<400>57atg gaa aac att gac cca aaa ctt tat cac cat acg cct acc gtc agt 48Met Glu Asn Ile Asp Pro Lys Leu Tyr His His Thr Pro Thr Val Ser1 5 10 15gtt cac gat aac cgt gga cta gct atc cgt aat att agt ttt cac cgc 96Val His Asp Asn Arg Gly Leu Ala Ile Arg Asn Ile Ser Phe His Arg20 25 30act acc gca gaa gca aat acc gat acc cgt att acc cgc cat caa tat144Thr Thr Ala Glu Ala Asn Thr Asp Thr Arg Ile Thr Arg His Gln Tyr35 40 45aat gcc ggc gga tat ttg aac caa agc att gat cct cgc ctg tat gac192Asn Ala Gly Gly Tyr Leu Asn Gln Ser Ile Asp Pro Arg Leu Tyr Asp50 55 60gcc aaa cag act aac aac gct gta caa ccg aat ttt atc tgg cga cat240Ala Lys Gln Thr Asn Asn Ala Val Gln Pro Ash Phe Ile Trp Arg His65 70 75 80aat ttg acc ggc aat atc ctg cga aca gag agc gtc gat gcc ggt cgg288Asn Leu Thr Gly Asn Ile Leu Arg Thr Glu Ser Val Asp Ala Gly Arg85 90 95
acg att acc ctc aac gat att gaa ggc cgc ccg gtg ttg acc atc aat336Thr Ile Thr Leu Asn Asp Ile Glu Gly Arg Pro Val Leu Thr Ile Asn100 105 110gca gcc ggt gtc cgg caa aac cat cgc tac gaa gat aac acc ctg ccc384Ala Ala Gly Val Arg Gln Asn His Arg Tyr Glu Asp Asn Thr Leu Pro115 120 125ggt cgc ctg ctc gct atc agc gaa caa gga cag gca gaa gag aaa acg432Gly Arg Leu Leu Ala Ile Ser Glu Gln Gly Gln Ala Glu Glu Lys Thr130 135 140acc gag cgc ctt atc tgg gcc ggc aat acg ccg caa gaa aaa gac cac480Thr Glu Arg Leu Ile Trp Ala Gly Asn Thr Pro Gln Glu Lys Asp His145 150 155 160aac ctt gcc ggt cag tgc gtc cgc cat tac gat acc gca gga ctc act528Asn Leu Ala Gly Gln Cys Val Arg His Tyr Asp Thr Ala Gly Leu Thr165 170 175caa ctc aac agc ctt gcc ctg acc ggc gcc gtt cta tca caa tct caa576Gln Leu Asn Ser Leu Ala Leu Thr Gly Ala Val Leu Ser Gln Ser Gln180 185 190caa ctg ctt acc gat aac cag gat gcc gac tgg aca ggt gaa gac cag624Gln Leu Leu Thr Asp Asn Gln Asp Ala Asp Trp Thr Gly Glu Asp Gln195 200 205agc ctc tgg caa caa aaa ctg agt agt gat gtc tat atc acc caa agt672Ser Leu Trp Gln Gln Lys Leu Ser Ser Asp Val Tyr Ile Thr Gln Ser210 215 220aac act gat gcc acc ggg gct tta ctg acc cag acc gat gcc aaa ggc720Asn Thr Asp Ala Thr Gly Ala Leu Leu Thr Gln Thr Asp Ala Lys Gly225 230 235 240aac att cag cgg ctg gcc tat gat gtg gcc ggg cag cta aaa ggg agt768Asn Ile Gln Arg Leu Ala Tyr Asp Val Ala Gly Gln Leu Lys Gly Ser245 250 255tgg tta aca ctc aaa ggt cag gcg gaa cag gtg att atc aaa tcg cta816Trp Leu Thr Leu Lys Gly Gln Ala Glu Gln Val Ile Ile Lys Ser Leu260 265 270
acc tac tcc gcc gcc ggg caa aaa tta cgt gaa gag cac ggt aac ggg 864Thr Tyr Ser Ala Ala Gly Gln Lys Leu Arg Glu Glu His Gly Asn Gly275 280 285att gtc act gaa tac agc tac gaa ccg gaa acc caa cgg ctt atc ggc 912Ile Val Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gln Arg Leu Ile Gly290 295 300att acc act cgc cgt cca tca gac gcc aag gtg ttg caa gac cta cgc 960Ile Thr Thr Arg Arg Pro Ser Asp Ala Lys Val Leu Gln Asp Leu Arg305 310 315 320tat caa tat gac cca gta ggc aat gtc att agt atc cgt aat gat gcg1008Tyr Gln Tyr Asp Pro Val Gly Asn Val Ile Ser Ile Arg Asn Asp Ala325 330 335gaa gcc act cgc ttt tgg cgc aat cag aaa gta gcc ccg gag aat agc1056Glu Ala Thr Arg Phe Trp Arg Asn Gln Lys Val Ala Pro Glu Asn Ser340 345 350tat acc tac gat tcc ctg tat cag ctt atc agc gcc acc ggg cgc gag1104Tyr Thr Tyr Asp Ser Leu Tyr Gln Leu Ile Ser Ala Thr Gly Arg Glu355 360 365atg gcc aat atc ggt cag caa agc aac caa ctt ccc tct ccg gcg cta1152Met Ala Asn Ile Gly Gln Gln Ser Asn Gln Leu Pro Ser Pro Ala Leu370 375 380cct tct gat aac aat acc tac acc aac tat act cgc act tat act tat1200Pro Ser Asp Asn Asn Thr Tyr Thr Asn Tyr Thr Arg Thr Tyr Thr Tyr385 390 395 400gac cgt ggc ggc aat ttg acg aaa att cag cat agt tca cca gcc gcg1248Asp Arg Gly Gly Asn Leu Thr Lys Ile Gln His Ser Ser Pro Ala Ala405 410 415caa aat aac tac acg acg gat ata acg gtt tca aat cgc agc aac cgc1296Gln Asn Asn Tyr Thr Thr Asp Ile Thr Val Ser Asn Arg Ser Asn Arg420 425 430gcg gta ctc agc aca ttg acc gca gat cca act caa gtc gat gcc tta1344Ala Val Leu Ser Thr Leu Thr Ala Asp Pro Thr Gln Val Asp Ala Leu435 440 445
ttt gat gcg gga ggc cat caa acc agc ttg tta tcc ggc caa gtt cta1392Phe Asp Ala Gly Gly His Gln Thr Ser Leu Leu Ser Gly Gln Val Leu450 455 460act tgg aca ccg cga ggc gaa ttg aaa caa gcc aac aat agc gca gga1440Thr Trp Thr Pro Arg Gly Glu Leu Lys Gln Ala Asn Asn Ser Ala Gly465 470 475 480aat gag tgg tat cgc tac gat agc aac ggc ata cgc cag cta aaa gtg1488Asn Glu Trp Tyr Arg Tyr Asp Ser Asn Gly Ile Arg Gln Leu Lys Val485 490 495aat gaa caa caa act cag aat atc ccg caa caa caa agg gta act tat1536Asn Glu Gln Gln Thr Gln Asn Ile Pro Gln Gln Gln Arg Val Thr Tyr500 505 510cta ccg ggg ctg gaa ata cgt aca acc cag aac aac gcc aca aca acal584Leu Pro Gly Leu Glu Ile Arg Thr Thr Gln Asn Asn Ala Thr Thr Thr515 520 525gaa gag tta cac gtt atc aca ctc ggt aaa gcc ggc cgc gcg caa gtc1632Glu Glu Leu His Val Ile Thr Leu Gly Lys Ala Gly Arg Ala Gln Val530 535 540cga gta ttg cat tgg gag agc ggt aaa cca gaa gat att aat aac aat1680Arg Val Leu His Trp Glu Ser Gly Lys Pro Glu Asp Ile Asn Asn Asn545 550 555 560cag ctt cgt tac agc tac gat aat ctt att ggc tcc agc caa ctt caa1728Gln Leu Arg Tyr Ser Tyr Asp Asn Leu Ile Gly Ser Ser Gln Leu Gln565 570 575tta gat agc gac gga caa att atc agt gaa gaa gaa tat tat cca ttt1776Leu Asp Ser Asp Gly Gln Ile Ile Ser Glu Glu Glu Tyr Tyr Pro Phe580 585 590ggt ggt aca gcg ctg tgg gcg gca agg aat caa acc gaa gcc agc tat1824Gly Gly Thr Ala Leu Trp Ala Ala Arg Asn Gln Thr Glu Ala Ser Tyr595 600 605aaa acc att cgt tat tct ggt aaa gag cgg gat gtt acc ggg ctg tat1872Lys Thr Ile Arg Tyr Ser Gly Lys Glu Arg Asp Val Thr Gly Leu Tyr610 615 620
tat tat ggc tac cgt tat tac caa ccg tgg gcg ggc aga tgg tta ggt1920Tyr Tyr Gly Tyr Arg Tyr Tyr Gln Pro Trp Ala Gly Arg Trp Leu Gly625 630 635 640gca gac ccg gca gga acc att gat gga ctg aat tta tat cgc atg gtg1968Ala Asp Pro Ala Gly Thr Ile Asp Gly Leu Asn Leu Tyr Arg Met Val645 650 655aga aat aac ccg gtg acg caa ttt gat gtt cag gga tta tca ccg gcc2016Arg Asn Asn Pro Val Thr Gln Phe Asp Val Gln Gly Leu Ser Pro Ala660 665 670aac aga aca gaa gaa gcg ata ata aaa cag ggt tcc ttt acg gga atg2064Asn Arg Thr Glu Glu Ala Ile Ile Lys Gln Gly Ser Phe Thr Gly Met675 680 685gaa gaa gct gtt tat aaa aaa atg gct aaa cct caa act ttc aaa cgc2112Glu Glu Ala Val Tyr Lys Lys Met Ala Lys Pro Gln Thr Phe Lys Arg690 695 700caa aga gct atc gct gcc caa aca gag caa gaa gcc cat gaa tca ttg2160Gln Arg Ala Ile Ala Ala Gln Thr Glu Gln Glu Ala His Glu Ser Leu705 710 715 720acc aac aac cct agt gta gat att agc cca att aaa aac tac acc aca2208Thr Asn Asn Pro Ser Val Asp Ile Ser Pro Ile Lys Asn Tyr Thr Thr725 730 735gat agc tca caa att aat gcc gcg ata agg gaa aat cgt att acg cca2256Asp Ser Ser Gln Ile Asn Ala Ala Ile Arg Glu Asn Arg Ile Thr Pro740 745 750gca gtg gaa agt tta gac gcc aca tta tct tcc cta caa gat aga caa2304Ala Val Glu Ser Leu Asp Ala Thr Leu Ser Ser Leu Gln Asp Arg Gln755 760 765atg agg gta act tat cgg gtg atg acc tat gta gat aat tcc acg cca2352Met Arg Val Thr Tyr Arg Val Met Thr Tyr Val Asp Asn Ser Thr Pro770 775 780tcg cct tgg cac tcg cca cag gaa gga aat agt att aat gtt ggt gat2400Ser Pro Trp His Ser Pro Gln Glu Gly Asn Ser Ile Asn Val Gly Asp785 790 795 800
atc gtt tcg gat aac gct tat tta tca aca tcg gcc cat cgt ggt ttt2448Ile Val Ser Asp Asn Ala Tyr Leu Ser Thr Ser Ala His Arg Gly Phe805 810 815ctg aat ttt gtt cac aaa aaa gaa acc agt gaa act cga tac gtc aag2496Leu Asn Phe Val His Lys Lys Glu Thr Ser Glu Thr Arg Tyr Val Lys820 825 830atg gca ttt tta acg aat gcg ggt gtc aat gtc cca gca gca tct atg2544Met Ala Phe Leu Thr Asn Ala Gly Val Asn Val Pro Ala Ala Ser Met835 840 845tat aat aat gct ggc gag gag caa gta ttt aaa atg gat tta aac gat2592Tyr Asn Asn Ala Gly Glu Glu Gln Val Phe Lys Met Asp Leu Asn Asp850 855 860tca aga aaa agc ctt gct gaa aaa tta aaa cta aga gtc agt gga cca2640Ser Arg Lys Ser Leu Ala Glu Lys Leu Lys Leu Arg Val Ser Gly Pro865 870 875 880caa tcg gga caa gcg gaa ata tta cta cct agg gaa aca cag ttc gaa2688Gln Ser Gly Gln Ala Glu Ile Leu Leu Pro Arg Glu Thr Gln Phe Glu885 890 895gtt gtt tca atg aaa cat caa ggc aga gat acc tat gta tta ttg caa2736Val Val Ser Met Lys His Gln Gly Arg Asp Thr Tyr Val Leu Leu Gln900 905 910gat att aac caa tcc gca gcc act cat aga aat gta cgt aac act tac2784Asp Ile Asn Gln Ser Ala Ala Thr His Arg Asn Val Arg Asn Thr Tyr915 920 925acc ggt aat ttc aaa tca tcc agt gca aat taa2817Thr Gly Ash Phe Lys Ser Ser Ser Ala Asn930 935<210>58<211>915<212>PRT<213>发光光杆状菌<400>58
Met Ser Ser Tyr Asn Ser Ala Ile Asp Gln Lys Thr Pro Ser Ile Lys1 5 10 15Val Leu Asp Asn Arg Lys Leu Asn Val Arg Thr Leu Glu Tyr Leu Arg20 25 30Thr Gln Ala Asp Glu Asn Ser Asp Glu Leu Ile Thr Phe Tyr Glu Phe35 40 45Asn Ile Pro Gly Phe Gln Val Lys Ser Thr Asp Pro Arg Lys Asn Lys50 55 60Asn Gln Ser Gly Pro Asn Phe Ile Arg Val Phe Asn Leu Ala Gly Gln65 70 75 80Val Leu Arg Glu Glu Ser Val Asp Ala Gly Arg Thr Ile Thr Leu Asn85 90 95Asp Ile Glu Ser Arg Pro Val Leu Ile Ile Asn Ala Thr Gly Val Arg100 105 110Gln Asn His Arg Tyr Glu Asp Asn Thr Leu Pro Gly Arg Leu Leu Ala115 120 125Ile Thr Glu Gln Val Gln Ala Gly Glu Lys Thr Thr Glu Arg Leu Ile130 135 140Trp Ala Gly Asn Thr Pro Gln Glu Lys Asp Tyr Asn Leu Ala Gly Gln145 150 155 160Cys Val Arg His Tyr Asp Thr Ala Gly Leu Thr Gln Leu Asn Ser Leu165 170 175
Ser Leu Ala Gly Val Val Leu Ser Gln Ser Gln Gln Leu Leu Val Asp180 185 190Asp Lys Asn Ala Asp Trp Thr Gly Glu Asp Gln Ser Leu Trp Gln Gln195 200 205Lys Leu Ser Ser Asp Val Tyr Thr Thr Gln Asn Lys Ala Asp Ala Thr210 215 220Gly Ala Leu Leu Thr Gln Thr Asp Ala Lys Gly Asn Ile Gln Arg Leu225 230 235 240Ala Tyr Asp Val Ala Gly Gln Leu Lys Gly Cys Trp Leu Thr Leu Lys245 250 255Gly Gln Ala Glu Gln Val Ile Ile Lys Ser Leu Thr Tyr Ser Ala Ala260 265 270Gly Gln Lys Leu Arg Glu Glu His Gly Asn Gly Val Ile Thr Glu Tyr275 280 285Ser Tyr Glu Pro Glu Thr Gln Arg Leu Ile Gly Ile Ala Thr Arg Arg290 295 300Pro Ser Asp Ala Lys Val Leu Gln Asp Leu Arg Tyr Gln Tyr Asp Pro305 310 315 320Val Gly Asn Val Ile Asn Ile Arg Asn Asp Ala Glu Ala Thr Arg Phe325 330 335Trp Arg Asn Gln Lys Val Val Pro Glu Asn Ser Tyr Thr Tyr Asp Ser340 345 350
Leu Tyr Gln Leu Ile Ser Ala Thr Gly Arg Glu Met Ala Asn Ile Gly355 360 365Gln Gln Asn Asn Gln Leu Pro Ser Pro Ala Leu Pro Ser Asp Asn Asn370 375 380Thr Tyr Thr Asn Tyr Thr Arg Ser Tyr Ser Tyr Asp His Ser Gly Asn385 390 395 400Leu Thr Gln Ile Arg His Ser Ser Pro Ala Thr Gln Asn Asn Tyr Thr405 410 415Val Ala Ile Thr Leu Ser Asn Arg Ser Asn Arg Gly Val Leu Ser Thr420 425 430Leu Thr Thr Asp Pro Asn Gln Val Asp Thr Leu Phe Asp Ala Gly Gly435 440 445His Gln Thr Ser Leu Leu Pro Gly Gln Thr Leu Ile Trp Thr Pro Arg450 455 460Gly Glu Leu Lys Gln Val Asn Asn Gly Pro Gly Asn Glu Trp Tyr Arg465 470 475 480Tyr Asp Ser Asn Gly Met Arg Gln Leu Lys Val Ser Glu Gln Pro Thr485 490 495Gln Asn Thr Thr Gln Gln Gln Arg Val Ile Tyr Leu Pro Gly Leu Glu500 505 510Leu Arg Thr Thr Gln Ser Asn Ala Thr Thr Thr Glu Glu Leu His Val515 520 525
Ile Thr Leu Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp530 535 540Glu Ser Gly Lys Pro Glu Asp Val Asn Asn Asn Gln Leu Arg Tyr Ser545 550 555 560Tyr Asp Asn Leu Ile Gly Ser Ser Gln Leu Glu Leu Asp Asn Gln Gly565 570 575Gln Ile Ile Ser Glu Glu Glu Tyr Tyr Pro Phe Gly Gly Thr Ala Leu580 585 590Trp Ala Ala Asn Ser Gln Thr Glu Ala Ser Tyr Lys Thr Ile Arg Tyr595 600 605Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg610 615 620Tyr Tyr Gln Pro Trp Ala Gly Arg Trp Leu Ser Ala Asp Pro Ala Gly625 630 635 640Thr Ile Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Val645 650 655Ser Leu Gln Asp Glu Asn Gly Leu Ala Pro Glu Lys Gly Lys Tyr Thr660 665 670Lys Glu Val Asn Phe Phe Asp Glu Leu Lys Phe Lys Leu Ala Ala Lys675 680 685Ser Ser His Val Val Lys Trp Asn Glu Lys Glu Ser Ser Tyr Thr Lys690 695 700
Asn Lys Ser Leu Lys Val Val Arg Val Gly Asp Ser Asp Pro Ser Gly705 710 715 720Tyr Leu Leu Ser His Glu Glu Leu Leu Lys Gly Ile Glu Lys Ser Gln725 730 735Ile Ile Tyr Ser Arg Leu Glu Glu Asn Ser Ser Leu Ser Glu Lys Ser740 745 750Lys Thr Asn Leu Ser Leu Gly Ser Glu Ile Ser Gly Tyr Met Ala Arg755 760 765Thr Ile Gln Asp Thr Ile Ser Glu Tyr Ala Glu Glu His Lys Tyr Arg770 775 780Ser Asn His Pro Asp Phe Tyr Ser Glu Thr Asp Phe Phe Ala Leu Met785 790 795 800Asp Lys Ser Glu Lys Asn Asp Tyr Ser Gly Glu Arg Lys Ile Tyr Ala805 810 815Ala Met Glu Val Lys Val Tyr His Asp Leu Lys Asn Lys Gln Ser Glu820 825 830Leu His Val Asn Tyr Ala Leu Ala His Pro Tyr Thr Gln Leu Ser Asn835 840 845Glu Glu Arg Ala Leu Leu Gln Glu Thr Glu Pro Ala Ile Ala Ile Asp850 855 860Arg Glu Tyr Asn Phe Lys Gly Val Gly Lys Phe Leu Thr Met Lys Ala865 870 875 880
Ile Lys Lys Ser Leu Lys Gly His Lys Ile Asn Arg Ile Ser Thr Glu885 890 895Ala Ile Asn Ile Arg Ser Ala Ala Ile Ala Glu Asn Leu Gly Met Arg900 905 910Arg Thr Ser915<210>59<211>2504<212>PRT<213>发光光杆状菌<400>59Met Gln Asn Ser Leu Ser Ser Thr Ile Asp Thr Ile Cys Gln Lys Leu1 5 10 15Gln Leu Thr Cys Pro Ala Glu Ile Ala Leu Tyr Pro Phe Asp Thr Phe20 25 30Arg Glu Lys Thr Arg Gly Met Val Asn Trp Gly Glu Ala Lys Arg Ile35 40 45Tyr Glu Ile Ala Gln Ala Glu Gln Asp Arg Ash Leu Leu His Glu Lys50 55 60Arg Ile Phe Ala Tyr Ala Asn Pro Leu Leu Lys Asn Ala Val Arg Leu65 70 75 80Gly Thr Arg Gln Met Leu Gly Phe Ile Gln Gly Tyr Ser Asp Leu Phe85 90 95
Gly Asn Arg Ala Asp Asn Tyr Ala Ala Pro Gly Ser Val Ala Ser Met100 105 110Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asn115 120 125Leu His Asp Ser Ser Ser Ile Tyr Tyr Leu Asp Lys Arg Arg Pro Asp130 135 140Leu Ala Ser Leu Met Leu Ser Gln Lys Asn Met Asp Glu Glu Ile Ser145 150 155 160Thr Leu Ala Leu Ser Asn Glu Leu Cys Leu Ala Gly Ile Glu Thr Lys165 170 175Thr Gly Lys Ser Gln Asp Glu Val Met Asp Met Leu Ser Thr Tyr Arg180 185 190Leu Ser Gly Glu Thr Pro Tyr His His Ala Tyr Glu Thr Val Arg Glu195 200 205Ile Val His Glu Arg Asp Pro Gly Phe Arg His Leu Ser Gln Ala Pro210 215 220Ile Val Ala Ala Lys Leu Asp Pro Val Thr Leu Leu Gly Ile Ser Ser225 230 235 240His Ile Ser Pro Glu Leu Tyr Asn Leu Leu Ile Glu Glu Ile Pro Glu245 250 255Lys Asp Glu Ala Ala Leu Asp Thr Leu Tyr Lys Thr Asn Phe Gly Asp260 265 270
Ile Thr Thr Ala Gln Leu Met Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr275 280 285Gly Val Ser Pro Glu Asp Ile Ala Tyr Val Thr Thr Ser Leu Ser His290 295 300Val Gly Tyr Ser Ser Asp Ile Leu Val Ile Pro Leu Val Asp Gly Val305 310 315 320Gly Lys Met Glu Val Val Arg Val Thr Arg Thr Pro Ser Asp Asn Tyr325 330 335Thr Ser Gln Thr Asn Tyr Ile Glu Leu Tyr Pro Gln Gly Gly Asp Asn340 345 350Tyr Leu Ile Lys Tyr Asn Leu Ser Asn Ser Phe Gly Leu Asp Asp Phe355 360 365Tyr Leu Gln Tyr Lys Asp Gly Ser Ala Asp Trp Thr Glu Ile Ala His370 375 380Asn Pro Tyr Pro Asp Met Val Ile Asn Gln Lys Tyr Glu Ser Gln Ala385 390 395 400Thr Ile Lys Arg Ser Asp Ser Asp Asn Ile Leu Ser Ile Gly Leu Gln405 410 415Arg Trp His Ser Gly Ser Tyr Asn Phe Ala Ala Ala Asn Phe Lys Ile420 425 430Asp Gln Tyr Ser Pro Lys Ala Phe Leu Leu Lys Met Asn Lys Ala Ile435 440 445
Arg Leu Leu Lys Ala Thr Gly Leu Ser Phe Ala Thr Leu Glu Arg Ile450 455 460Val Asp Ser Val Asn Ser Thr Lys Ser Ile Thr Val Glu Val Leu Asn465 470 475 480Lys Val Tyr Arg Val Lys Phe Tyr Ile Asp Arg Tyr Gly Ile Ser Glu485 490 495Glu Thr Ala Ala Ile Leu Ala Asn Ile Asn Ile Ser Gln Gln Ala Val500 505 510Gly Asn Gln Leu Ser Gln Phe Glu Gln Leu Phe Asn His Pro Pro Leu515 520 525Asn Gly Ile Arg Tyr Glu Ile Ser Glu Asp Asn Ser Lys His Leu Pro530 535 540Asn Pro Asp Leu Asn Leu Lys Pro Asp Ser Thr Gly Asp Asp Gln Arg545 550 555 560Lys Ala Val Leu Lys Arg Ala Phe Gln Val Asn Ala Ser Glu Leu Tyr565 570 575Gln Met Leu Leu Ile Thr Asp Arg Lys Glu Asp Gly Val Ile Lys Asn580 585 590Asn Leu Glu Asn Leu Ser Asp Leu Tyr Leu Val Ser Leu Leu Ala Gln595 600 605Ile His Asn Leu Thr Ile Ala Glu Leu Asn Ile Leu Leu Val Ile Cys610 615 620
Gly Tyr Gly Asp Thr Asn Ile Tyr Gln Ile Thr Asp Asp Asn Leu Ala625 630 635 640Lys Ile Val Glu Thr Leu Leu Trp Ile Thr Gln Trp Leu Lys Thr Gln645 650 655Lys Trp Thr Val Thr Asp Leu Phe Leu Met Thr Thr Ala Thr Tyr Ser660 665 670Thr Thr Leu Thr Pro Glu Ile Ser Asn Leu Thr Ala Thr Leu Ser Ser675 680 685Thr Leu His Gly Lys Glu Ser Leu Ile Gly Glu Asp Leu Lys Arg Ala690 695 700Met Ala Pro Cys Phe Thr Ser Ala Leu His Leu Thr Ser Gln Glu Val705 710 715 720Ala Tyr Asp Leu Leu Leu Trp Ile Asp Gln Ile Gln Pro Ala Gln Ile725 730 735Thr Val Asp Gly Phe Trp Glu Glu Val Gln Thr Thr Pro Thr Ser Leu740 745 750Lys Val Ile Thr Phe Ala Gln Val Leu Ala Gln Leu Ser Leu Ile Tyr755 760 765Arg Arg Ile Gly Leu Ser Glu Thr Glu Leu Ser Leu Ile Val Thr Gln770 775 780Ser Ser Leu Leu Val Ala Gly Lys Ser Ile Leu Asp His Gly Leu Leu785 790 795 800
Thr Leu Met Ala Leu Glu Gly Phe His Thr Trp Val Asn Gly Leu Gly805 810 815Gln His Ala Ser Leu Ile Leu Ala Ala Leu Lys Asp Gly Ala Leu Thr820 825 830Val Thr Asp Val Ala Gln Ala Met Asn Lys Glu Glu Ser Leu Leu Gln835 840 845Met Ala Ala Asn Gln Val Glu Lys Asp Leu Thr Lys Leu Thr Ser Trp850 855 860Thr Gln Ile Asp Ala Ile Leu Gln Trp Leu Gln Met Ser Ser Ala Leu865 870 875 880Ala Val Ser Pro Leu Asp Leu Ala Gly Met Met Ala Leu Lys Tyr Gly885 890 895Ile Asp His Asn Tyr Ala Ala Trp Gln Ala Ala Ala Ala Ala Leu Met900 905 910Ala Asp His Ala Asn Gln Ala Gln Lys Lys Leu Asp Glu Thr Phe Ser915 920 925Lys Ala Leu Cys Asn Tyr Tyr Ile Asn Ala Val Val Asp Ser Ala Ala930 935 940Gly Val Arg Asp Arg Asn Gly Leu Tyr Thr Tyr Leu Leu Ile Asp Asn945 950 955 960Gln Val Ser Ala Asp Val Ile Thr Ser Arg Ile Ala Glu Ala Ile Ala965 970 975
Gly Ile Gln Leu Tyr Val Asn Arg Ala Leu Asn Arg Asp Glu Gly Gln980 985 990Leu Ala Ser Asp Val Ser Thr Arg Gln Phe Phe Thr Asp Trp Glu Arg995 10001005Tyr Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Glu Leu Val1010 1015 1020Tyr Tyr Pro Glu Asn Tyr Val Asp Pro Thr Gln Arg Ile Gly Gln1025 1030 1035Thr Lys Met Met Asp Ala Leu Leu Gln Ser Ile Asn Gln Ser Gln1040 1045 1050Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Lys Thr Tyr Leu Thr1055 1060 1065Ser Phe Glu Gln Val Ala Asn Leu Lys Val Ile Ser Ala Tyr His1070 1075 1080Asp Asn Val Asn Val Asp Gln Gly Leu Thr Tyr Phe Ile Gly Ile1085 1090 1095Asp Gln Ala Ala Pro Gly Thr Tyr Tyr Trp Arg Ser Val Asp His1100 1105 1110Ser Lys Cys Glu Asn Gly Lys Phe Ala Ala Asn Ala Trp Gly Glu1115 1120 1125Trp Asn Lys Ile Thr Cys Ala Val Asn Pro Trp Lys Asn Ile Ile1130 1135 1140
Arg Pro Val Val Tyr Met Ser Arg Leu Tyr Leu Leu Trp Leu Glu1145 1150 1155Gln Gln Ser Lys Lys Ser Asp Asp Gly Lys Thr Thr Ile Tyr Gln1160 1165 1170Tyr Asn Leu Lys Leu Ala His Ile Arg Tyr Asp Gly Ser Trp Asn1175 1180 1185Thr Pro Phe Thr Phe Asp Val Thr Glu Lys Val Lys Asn Tyr Thr1190 1195 1200Ser Ser Thr Asp Ala Ala Glu Ser Leu Gly Leu Tyr Cys Thr Gly1205 1210 1215Tyr Gln Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Ser Met Gln1220 1225 1230Ser Ser Tyr Ser Ser Tyr Thr Asp Asn Asn Ala Pro Val Thr Gly1235 1240 1245Leu Tyr Ile Phe Ala Asp Met Ser Ser Asp Asn Met Thr Asn Ala1250 1255 1260Gln Ala Thr Asn Tyr Trp Asn Asn Ser Tyr Pro Gln Phe Asp Thr1265 1270 1275Val Met Ala Asp Pro Asp Ser Asp Asn Lys Lys Val Ile Thr Arg1280 1285 1290Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr Glu Ile Pro Ser Ser1295 1300 1305
Val Thr Ser Asn Ser Asn Tyr Ser Trp Gly Asp His Ser Leu Thr1310 1315 1320Met Leu Tyr Gly Gly Ser Val Pro Asn Ile Thr Phe Glu Ser Ala1325 1330 1335Ala Glu Asp Leu Arg Leu Ser Thr Asn Met Ala Leu Ser Ile Ile1340 1345 1350His Asn Gly Tyr Ala Gly Thr Arg Arg Ile Gln Cys Asn Leu Met1355 1360 1365Lys Gln Tyr Ala Ser Leu Gly Asp Lys Phe Ile Ile Tyr Asp Ser1370 1375 1380Ser Phe Asp Asp Ala Asn Arg Phe Asn Leu Val Pro Leu Phe Lys1385 1390 1395Phe Gly Lys Asp Glu Asn Ser Asp Asp Ser Ile Cys Ile Tyr Asn1400 1405 1410Glu Asn Pro Ser Ser Glu Asp Lys Lys Trp Tyr Phe Ser Ser Lys1415 1420 1425Asp Asp Asn Lys Thr Ala Asp Tyr Asn Gly Gly Thr Gln Cys Ile1430 1435 1440Asp Ala Gly Thr Ser Asn Lys Asp Phe Tyr Tyr Asn Leu Gln Glu1445 1450 1455Ile Glu Val Ile Ser Val Thr Gly Gly Tyr Trp Ser Ser Tyr Lys1460 1465 1470
Ile Ser Asn Pro Ile Asn Ile Asn Thr Gly Ile Asp Ser Ala Lys1475 1480 1485Val Lys Val Thr Val Lys Ala Gly Gly Asp Asp Gln Ile Phe Thr1490 1495 1500Ala Asp Asn Ser Thr Tyr Val Pro Gln Gln Pro Ala Pro Ser Phe1505 1510 1515Glu Glu Met Ile Tyr Gln Phe Asn Asn Leu Thr Ile Asp Cys Lys1520 1525 1530Asn Leu Asn Phe Ile Asp Asn Gln Ala His Ile Glu Ile Asp Phe1535 1540 1545Thr Ala Thr Ala Gln Asp Gly Arg Phe Leu Gly Ala Glu Thr Phe1550 1555 1560Ile Ile Pro Val Thr Lys Lys Val Leu Gly Thr Glu Asn Val Ile1565 1570 1575Ala Leu Tyr Ser Glu Asn Asn Gly Val Gln Tyr Met Gln Ile Gly1580 1585 1590Ala Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Gln Gln Leu Val1595 1600 1605Ser Arg Ala Asn Arg Gly Ile Asp Ala Val Leu Ser Met Glu Thr1610 1615 1620Gln Asn Ile Gln Glu Pro Gln Leu Gly Ala Gly Thr Tyr Val Gln1625 1630 1635
Leu Val Leu Asp Lys Tyr Asp Glu Ser Ile His Gly Thr Asn Lys1640 1645 1650Ser Phe Ala Ile Glu Tyr Val Asp Ile Phe Lys Glu Asn Asp Ser1655 1660 1665Phe Val Ile Tyr Gln Gly Glu Leu Ser Glu Thr Ser Gln Thr Val1670 1675 1680Val Lys Val Phe Leu Ser Tyr Phe Ile Glu Ala Thr Gly Asn Lys1685 1690 1695Asn His Leu Trp Val Arg Ala Lys Tyr Gln Lys Glu Thr Thr Asp1700 1705 1710Lys Ile Leu Phe Asp Arg Thr Asp Glu Lys Asp Pro His Gly Trp1715 1720 1725Phe Leu Ser Asp Asp His Lys Thr Phe Ser Gly Leu Ser Ser Ala1730 1735 1740Gln Ala Leu Lys Asn Asp Ser Glu Pro Met Asp Phe Ser Gly Ala1745 1750 1755Asn Ala Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro Met Met1760 1765 1770Met Ala His Arg Leu Leu Gln Glu Gln Asn Phe Asp Ala Ala Asn1775 1780 1785His Trp Phe Arg Tyr Val Trp Ser Pro Ser Gly Tyr Ile Val Asp1790 1795 1800
Gly Lys Ile Ala Ile Tyr His Trp Asn Val Arg Pro Leu Glu Glu1805 1810 1815Asp Thr Ser Trp Asn Ala Gln Gln Leu Asp Ser Thr Asp Pro Asp1820 1825 1830Ala Val Ala Gln Asp Asp Pro Met His Tyr Lys Val Ala Thr Phe1835 1840 1845Met Ala Thr Leu Asp Leu Leu Met Ala Arg Gly Asp Ala Ala Tyr1850 1855 1860Arg Gln Leu Glu Arg Asp Thr Leu Ala Glu Ala Lys Met Trp Tyr1865 1870 1875Thr Gln Ala Leu Asn Leu Leu Gly Asp Glu Pro Gln Val Met Leu1880 1885 1890Ser Thr Thr Trp Ala Asn Pro Thr Leu Gly Asn Ala Ala Ser Lys1895 1900 1905Thr Thr Gln Gln Val Arg Gln Gln Val Leu Thr Gln Leu Arg Leu1910 1915 1920Asn Ser Arg Val Lys Thr Pro Leu Leu Gly Thr Ala Asn Ser Leu1925 1930 1935Thr Ala Leu Phe Leu Pro Gln Glu Asn Ser Lys Leu Lys Gly Tyr1940 1945 1950Trp Arg Thr Leu Ala Gln Arg Met Phe Asn Leu Arg His Asn Leu1955 1960 1965
Ser Ile Asp Gly Gln Pro Leu Ser Leu Pro Leu Tyr Ala Lys Pro1970 1975 1980Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser Ala Ser Gln1985 1990 1995Gly Gly Ala Asp Leu Pro Lys Ala Pro Leu Thr Ile His Arg Phe2000 2005 2010Pro Gln Met Leu Glu Gly Ala Arg Gly Leu Val Asn Gln Leu Ile2015 2020 2025Gln Phe Gly Ser Ser Leu Leu Gly Tyr Ser Glu Arg Gln Asp Ala2030 2035 2040Glu Ala Met Ser Gln Leu Leu Gln Thr Gln Ala Ser Glu Leu Ile2045 2050 2055Leu Thr Ser Ile Arg Met Gln Asp Asn Gln Leu Ala Glu Leu Asp2060 2065 2070Ser Glu Lys Thr Ala Leu Gln Val Ser Leu Ala Gly Val Gln Gln2075 2080 2085Arg Phe Asp Ser Tyr Ser Gln Leu Tyr Glu Glu Asn Ile Asn Ala2090 2095 2100Gly Glu Gln Arg Ala Leu Ala Leu Arg Ser Glu Ser Ala Ile Glu2105 2110 2115Ser Gln Gly Ala Gln Ile Ser Arg Met Ala Gly Ala Gly Val Asp2120 2125 2130
Met Ala Pro Asn Ile Phe Gly Leu Ala Asp Gly Gly Met His Tyr2135 2140 2145Gly Ala Ile Ala Tyr Ala Ile Ala Asp Gly Ile Glu Leu Ser Ala2150 2155 2160Ser Ala Lys Met Val Asp Ala Glu Lys Val Ala Gln Ser Glu Ile2165 2170 2175Tyr Arg Arg Arg Arg Gln Glu Trp Lys Ile Gln Arg Asp Asn Ala2180 2185 2190Gln Ala Glu Ile Asn Gln Leu Asn Ala Gln Leu Glu Ser Leu Ser2195 2200 2205Ile Arg Arg Glu Ala Ala Glu Met Gln Lys Glu Tyr Leu Lys Thr2210 2215 2220Gln Gln Ala Gln Ala Gln Ala Gln Leu Thr Phe Leu Arg Ser Lys2225 2230 2235Phe Ser Asn Gln Ala Leu Tyr Ser Trp Leu Arg Gly Arg Leu Ser2240 2245 2250Gly Ile Tyr Phe Gln Phe Tyr Asp Leu Ala Val Ser Arg Cys Leu2255 2260 2265Met Ala Glu Gln Ser Tyr Gln Trp Glu Ala Asn Asp Asn Ser Ile2270 2275 2280Ser Phe Val Lys Pro Gly Ala Trp Gln Gly Thr Tyr Ala Gly Leu2285 2290 2295
Leu Cys Gly Glu Ala Leu Ile Gln Asn Leu Ala Gln Met Glu Glu2300 2305 2310Ala Tyr Leu Lys Trp Glu Ser Arg Ala Leu Glu Val Glu Arg Thr2315 2320 2325Val Ser Leu Ala Val Val Tyr Asp Ser Leu Glu Gly Asn Asp Arg2330 2335 2340Phe Asn Leu Ala Glu Gln Ile Pro Ala Leu Leu Asp Lys Gly Glu2345 2350 2355Gly Thr Ala Gly Thr Lys Glu Asn Gly Leu Ser Leu Ala Asn Ala2360 2365 2370Ile Leu Ser Ala Ser Val Lys Leu Ser Asp Leu Lys Leu Gly Thr2375 2380 2385Asp Tyr Pro Asp Ser Ile Val Gly Ser Asn Lys Val Arg Arg Ile2390 2395 2400Lys Gln Ile Ser Val Ser Leu Pro Ala Leu Val Gly Pro Tyr Gln2405 2410 2415Asp Val Gln Ala Met Leu Ser Tyr Gly Gly Ser Thr Gln Leu Pro2420 2425 2430Lys Gly Cys Ser Ala Leu Ala Val Ser His Gly Thr Asn Asp Ser2435 2440 2445Gly Gln Phe Gln Leu Asp Phe Asn Asp Gly Lys Tyr Leu Pro Phe2450 2455 2460
Glu Gly Ile Ala Leu Asp Asp Gln Gly Thr Leu Asn Leu Gln Phe2465 2470 2475Pro Ash Ala Thr Asp Lys Gln Lys Ala Ile Leu Gln Thr Met Ser2480 2485 2490Asp Ile Ile Leu His Ile Arg Tyr Thr Ile Arg2495 2500<210>60<211>1428<212>PRT<213>嗜线虫沙雷菌(Serratia entomophila)<400>60Met Gln Asn His Gln Asp Met Ala Ile Thr Ala Pro Thr Leu Pro Ser1 5 10 15Gly Gly Gly Ala Val Thr Gly Leu Lys Gly Asp Ile Ala Ala Ala Gly20 25 30Pro Asp Gly Ala Ala Thr Leu Ser Ile Pro Leu Pro Val Ser Pro Gly35 40 45Arg Gly Tyr Ala Pro Thr Gly Ala Leu Asn Tyr His Ser Arg Ser Gly50 55 60Asn Gly Pro Phe Gly Ile Gly Trp Gly Ile Gly Gly Ala Ala Val Gln65 70 75 80Arg Arg Thr Arg Asn Gly Ala Pro Thr Tyr Asp Asp Thr Asp Glu Phe85 90 95Thr Gly Pro Asp Gly Glu Val Leu Val Pro Ala Leu Thr Ala Ala Gly
100 105 110Thr Gln Glu Ala Arg Gln Ala Thr Ser Leu Leu Gly Ile Asn Pro Gly115 120 125Gly Ser Phe Asn Val Gln Val Tyr Arg Ser Arg Thr Glu Gly Ser Leu130 135 140Ser Arg Leu Glu Arg Trp Leu Pro Ala Asp Glu Thr Glu Thr Glu Phe145 150 155 160Trp Val Leu Tyr Thr Pro Asp Gly Gln Val Ala Leu Leu Gly Arg Asn165 170 175Ala Gln Ala Arg Ile Ser Asn Pro Thr Ala Pro Thr Gln Thr Ala Val180 185 190Trp Leu Met Glu Ser Ser Val Ser Leu Thr Gly Glu Gln Met Tyr Tyr195 200 205Gln Tyr Arg Ala Glu Asp Asp Asp Gly Cys Asp Glu Ala Glu Arg Asp210 215 220Ala His Pro Gln Ala Gly Ala Gln Arg Tyr Pro Val Ala Val Trp Tyr225 230 235 240Gly Asn Arg Gln Ala Ala Arg Thr Leu Pro Ala Leu Val Ser Thr Pro245 250 255Ser Met Asp Ser Trp Leu Phe Ile Leu Val Phe Asp Tyr Gly Glu Arg260 265 270Ser Ser Val Leu Ser Glu Ala Pro Ala Trp Gln Thr Pro Gly Ser Gly
275 280 285Glu Trp Leu Cys Arg Gln Asp Cys Phe Ser Gly Tyr Glu Phe Gly Phe290 295 300Asn Leu Arg Thr Arg Arg Leu Cys Arg Gln Val Leu Met Phe His Tyr305 310 315 320Leu Gly Val Leu Ala Gly Ser Ser Gly Ala Asn Asp Ala Pro Ala Leu325 330 335Ile Ser Arg Leu Leu Leu Asp Tyr Arg Glu Ser Pro Ser Leu Ser Leu340 345 350Leu Glu Asn Val His Gln Val Ala Tyr Glu Ser Asp Gly Thr Ser Cys355 360 365Ala Leu Pro Ala Leu Ala Leu Gly Trp Gln Thr Phe Thr Pro Pro Thr370 375 380Leu Ser Ala Trp Gln Thr Arg Asp Asp Met Gly Lys Leu Ser Leu Leu385 390 395 400Gln Pro Tyr Gln Leu Val Asp Leu Asn Gly Glu Gly Val Val Gly Ile405 410 415Leu Tyr Gln Asp Ser Gly Ala Trp Trp Tyr Arg Glu Pro Val Arg Gln420 425 430Ser Gly Asp Asp Pro Asp Ala Val Thr Trp Gly Ala Ala Ala Ala Leu435 440 445Pro Thr Met Pro Ala Leu His Asn Ser Gly Ile Leu Ala Asp Leu Asn
450 455 460Gly Asp Gly Arg Leu Glu Trp Val Val Thr Ala Pro Gly Val Ala Gly465 470 475 480Met Tyr Asp Arg Thr Pro Gly Arg Asp Trp Leu His Phe Thr Pro Leu485 490 495Ser Ala Leu Pro Val Glu Tyr Ala His Pro Lys Ala Val Leu Ala Asp500 505 510Ile Leu Gly Ala Gly Leu Thr Asp Met Val Leu Ile Gly Pro Arg Ser515 520 525Val Arg Leu Tyr Ser Gly Lys Asn Asp Gly Trp Asn Lys Gly Glu Thr530 535 540Val Gln Gln Thr Glu Arg Leu Thr Leu Pro Val Pro Gly Val Asp Pro545 550 555 560Arg Thr Leu Val Ala Phe Ser Asp Met Ala Gly Ser Gly Gln Gln His565 570 575Leu Thr Glu Val Arg Ala Asn Gly Val Arg Tyr Trp Pro Asn Leu Gly580 585 590His Gly Arg Phe Gly Gln Pro Val Asn Ile Pro Gly Phe Ser Gln Ser595 600 605Val Thr Thr Phe Asn Pro Asp Gln Ile Leu Leu Ala Asp Thr Asp Gly610 615 620Ser Gly Thr Thr Asp Leu Ile Tyr Ala Met Ser Asp Arg Leu Val Ile
625 630 635 640Tyr Phe Asn Gln Ser Gly Asn Tyr Phe Ala Glu Pro His Thr Leu Leu645 650 655Leu Pro Lys Gly Val Arg Tyr Asp Arg Thr Cys Ser Leu Gln Val Ala660 665 670Asp Ile Gln Gly Leu Gly Val Pro Ser Leu Leu Leu Thr Val Pro His675 680 685Val Ala Pro His His Trp Val Cys His Leu Ser Ala Asp Lys Pro Trp690 695 700Leu Leu Asn Gly Met Asn Asn Asn Met Gly Ala Arg His Ala Leu His705 710 715 720Tyr Arg Ser Ser Val Gln Phe Trp Leu Asp Glu Lys Ala Glu Ala Leu725 730 735Ala Ala Gly Ser Ser Pro Ala Cys Tyr Leu Pro Phe Thr Leu His Thr740 745 750Leu Trp Arg Ser Val Val Gln Asp Glu Ile Thr Gly Asn Arg Leu Val755 760 765Ser Asp Val Leu Tyr Arg His Gly Val Trp Asp Gly Gln Glu Arg Glu770 775 780Phe Arg Gly Phe Gly Phe Val Glu Ile Arg Asp Thr Asp Thr Leu Ala785 790 795 800Ser Gln Gly Thr Ala Thr Glu Leu Ser Met Pro Ser Val Ser Arg Asn
805 810 815Trp Tyr Ala Thr Gly Val Pro Ala Val Asp Glu Arg Leu Pro Glu Thr820 825 830Tyr Trp Gln Asn Asp Ala Ala Ala Phe Ala Asp Phe Ala Thr Arg Phe835 840 845Thr Val Gly Ser Gly Glu Asp Glu Gln Thr Tyr Thr Pro Asp Asp Ser850 855 860Lys Thr Phe Trp Leu Gln Arg Ala Leu Lys Gly Ile Leu Leu Arg Ser865 870 875 880Glu Leu Tyr Gly Ala Asp Gly Ser Ser Gln Ala Asp Ile Pro Tyr Ser885 890 895Val Thr Glu Ser Arg Pro Gln Val Arg Leu Val Glu Ala Asn Gly Asp900 905 910Tyr Pro Val Val Trp Pro Met Gly Ala Glu Ser Arg Thr Ser Val Tyr915 920 925Glu Arg Tyr His Asn Asp Pro Gln Cys Gln Gln Gln Ala Val Leu Leu930 935 940Ser Asp Glu Tyr Gly Phe Pro Leu Arg Gln Val Ser Val Asn Tyr Pro945 950 955 960Arg Arg Pro Pro Ser Ala Asp Asn Pro Tyr Pro Ala Ser Leu Pro Ala965 970 975Thr Leu Phe Ala Asn Ser Tyr Asp Glu Gln Gln Gln Ile Leu Arg Leu
980 985 990Gly Leu Gln Gln Ser Ser Ala His His Leu Val Ser Leu Ser Glu Gly995 10001005His Trp Leu Leu Gly Leu Ala Glu Ala Ser Arg Asp Asp Val Phe1010 1015 1020Thr Tyr Ser Ala Asp Asn Val Pro Glu Gly Gly Leu Thr Leu Glu1025 1030 1035His Leu Leu Ala Pro Glu Ser Leu Val Ser Asp Ser Gln Val Gly1040 1045 1050Thr Leu Ala Gly Gln Gln Gln Val Trp Tyr Leu Asp Ser Gln Asp1055 1060 1065Val Ala Thr Val Ala Ala Pro Pro Leu Pro Pro Lys Val Ala Phe1070 1075 1080Ile Glu Thr Ala Val Leu Asp Glu Gly Met Val Ser Ser Leu Ala1085 1090 1095Ala Tyr Ile Val Asp Glu His Leu Glu Gln Ala Gly Tyr Arg Gln1100 1105 1110Ser Gly Tyr Leu Phe Pro Arg Gly Arg Glu Ala Glu Gln Ala Leu1115 1120 1125Trp Thr Gln Cys Gln Gly Tyr Val Thr Tyr Ala Gly Ala Glu His1130 1135 1140Phe Trp Leu Pro Leu Ser Phe Arg Asp Ser Met Leu Thr Gly Pro
1145 1150 1155Val Thr Val Thr Arg Asp Ala Tyr Asp Cys Val Ile Thr Gln Trp1160 1165 1170Gln Asp Ala Ala Gly Ile Val Thr Thr Ala Asp Tyr Asp Trp Arg1175 1180 1185Phe Leu Thr Pro Val Arg Val Thr Asp Pro Asn Asp Asn Leu Gln1190 1195 1200Ser Val Thr Leu Asp Ala Leu Gly Arg Val Thr Thr Leu Arg Phe1205 1210 1215Trp Gly Thr Glu Asn Gly Ile Ala Thr Gly Tyr Ser Asp Ala Thr1220 1225 1230Leu Ser Val Pro Asp Gly Ala Ala Ala Ala Leu Ala Leu Thr Ala1235 1240 1245Pro Leu Pro Val Ala Gln Cys Leu Val Tyr Val Thr Asp Ser Trp1250 1255 1260Gly Asp Asp Asp Asn Glu Lys Met Pro Pro His Val Val Val Leu1265 1270 1275Ala Thr Asp Arg Tyr Asp Ser Asp Thr Gly Gln Gln Val Arg Gln1280 1285 1290Gln Val Thr Phe Ser Asp Gly Phe Gly Arg Glu Leu Gln Ser Ala1295 1300 1305Thr Arg Gln Ala Glu Gly Asn Ala Trp Gln Arg Gly Arg Asp Gly
1310 1315 1320Lys Leu Val Thr Ala Ser Asp Gly Leu Pro Val Thr Val Ala Thr1325 1330 1335Asn Phe Arg Trp Ala Val Thr Gly Arg Ala Glu Tyr Asp Asn Lys1340 1345 1350Gly Leu Pro Val Arg Val Tyr Gln Pro Tyr Phe Leu Asp Ser Trp1355 1360 1365Gln Tyr Val Ser Asp Asp Ser Ala Arg Gln Asp Leu Tyr Ala Asp1370 1375 1380Thr His Phe Tyr Asp Pro Thr Ala Arg Glu Trp Gln Val Ile Thr1385 1390 1395Ala Lys Gly Glu Arg Arg Gln Val Leu Tyr Thr Pro Trp Phe Val1400 1405 1410Val Ser Glu Asp Glu Asn Asp Thr Val Gly Leu Ash Asp Ala Ser1415 1420 1425<210>61<211>973<212>PRT<213>嗜线虫沙雷菌<400>61Met Ser Thr Ser Leu Phe Ser Ser Thr Pro Ser Val Ala Val Leu Asp1 5 10 15Asn Arg Gly Leu Leu Val Arg Glu Leu Gin Tyr Tyr Arg His Pro Asp20 25 30
Thr Pro Glu Glu Thr Asp Glu Arg Ile Thr Cys His Gln His Asp Glu35 40 45Arg Gly Ser Leu Ser Gln Ser Ala Asp Pro Arg Leu His Ala Ala Gly50 55 60Leu Thr Asn Phe Thr Tyr Leu Asn Ser Leu Thr Gly Thr Val Leu Gln65 70 75 80Ser Val Ser Ala Asp Ala Gly Thr Ser Leu Glu Leu Ser Asp Ala Ala85 90 95Gly Arg Ala Phe Leu Ala Val Thr Gly Ala Gly Thr Glu Asp Ala Val100 105 110Thr Arg Thr Trp Gln Tyr Glu Asp Asp Thr Leu Pro Gly Arg Pro Leu115 120 125Ser Ile Thr Glu Gln Val Thr Gly Glu Ala Ala Gln Ile Thr Glu Arg130 135 l40Phe Val Tyr Ala Gly Asn Thr Asp Ala Glu Lys Ile Leu Asn Leu Ala145 150 155 160Gly Gln Cys Val Ser His Tyr Asp Thr Ala Gly Leu Val Gln Thr Asp165 170 175Ser Ile Ala Leu Ser Gly Val Pro Leu Ala Val Thr Arg Gln Leu Leu180 185 190Pro Asp Ala Ala Gly Ala Asn Trp Met Gly Glu Asp Ala Ser Ala Trp195 200 205
Asn Asp Leu Leu Asp Gly Glu Thr Phe Phe Thr Gln Thr His Ala Asp210 215 220Ala Thr Gly Ala Val Leu Ser Ile Thr Asp Ala Lys Gly Asn Leu Gln225 230 235 240Arg Val Ala Tyr Asp Val Ala Gly Leu Leu Ser Gly Ser Trp Leu Thr245 250 255Leu Lys Asp Gly Thr Glu Gln Val Ile Val Ala Ser Leu Thr Tyr Ser260 265 270Ala Ala Gly Lys Lys Leu Arg Glu Glu His Gly Asn Gly Val Val Thr275 280 285Ser Tyr Ile Tyr Glu Pro Glu Thr Gln Arg Leu Thr Gly Ile Lys Thr290 295 300Glu Arg Pro Ser Gly His Val Ala Gly Ala Lys Val Leu Gln Asp Leu305 310 315 320Arg Tyr Thr Tyr Asp Pro Val Gly Asn Val Leu Ser Val Asn Asn Asp325 330 335Ala Glu Glu Thr Arg Phe Trp Arg Asn Gln Lys Val Val Pro Glu Asn340 345 350Thr Tyr Ile Tyr Asp Ser Leu Tyr Gln Leu Val Ser Ala Thr Gly Arg355 360 365Glu Met Ala Asn Ala Gly Gln Gln Gly Asn Asp Leu Pro Ser Ala Thr370 375 380
Ala Pro Leu Pro Thr Asp Ser Ser Ala Tyr Thr Asn Tyr Thr Arg Thr385 390 395 400Tyr Arg Tyr Asp Arg Gly Gly Asn Leu Thr Gln Met Arg His Ser Ala405 410 415Pro Ala Thr Asn Asn Asn Tyr Thr Thr Asp Ile Thr Val Ser Asp Arg420 425 430Ser Asn Arg Ala Val Leu Ser Thr Leu Ala Glu Val Pro Ser Asp Val435 440 445Asp Met Leu Phe Ser Ala Gly Gly His Gln Lys His Leu Gln Pro Gly450 455 460Gln Ala Leu Val Trp Thr Pro Arg Gly Glu Leu Gln Lys Val Thr Pro465 470 475 480Val Val Arg Asp Gly Gly Ala Asp Asp Ser Glu Ser Tyr Arg Tyr Asp485 490 495Ala Gly Ser Gln Arg Ile Ile Lys Thr Gly Thr Arg Gln Thr Gly Asn500 505 510Asn Val Gln Thr Gln Arg Val Val Tyr Leu Pro Gly Leu Glu Leu Arg515 520 525Ile Met Ala Asn Gly Val Thr Glu Lys Glu Ser Leu Gln Val Ile Thr530 535 540Val Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp Glu Ile545 550 555 560
Gly Lys Pro Asp Asp Leu Asp Glu Asp Ser Val Arg Tyr Ser Tyr Asp565 570 575Asn Leu Val Gly Ser Ser Gln Leu Glu Leu Asp Arg Glu Gly Tyr Leu580 585 590Ile Ser Glu Glu Glu Phe Tyr Pro Tyr Gly Gly Thr Ala Val Leu Thr595 600 605Ala Arg Ser Glu Val Glu Ala Asp Tyr Lys Thr Ile Arg Tyr Ser Gly610 615 620Lys Glu Arg Asp Ala Thr Gly Leu Asp Tyr Tyr Gly Tyr Arg Tyr Tyr625 630 635 640Gln Pro Trp Ala Gly Arg Trp Leu Ser Thr Asp Pro Ala Gly Thr Val645 650 655Asp Gly Leu Asn Leu Phe Arg Met Val Arg Asn Asn Pro Val Thr Leu660 665 670Phe Asp Ser Asn Gly Arg Ile Ser Thr Gly Gln Glu Ala Arg Arg Leu675 680 685Val Gly Glu Ala Phe Val His Pro Leu His Met Pro Val Phe Glu Arg690 695 700Ile Ser Val Glu Arg Lys Ile Ser Met Ser Val Arg Glu Ala Gly Ile705 710 715 720Tyr Thr Ile Ser Ala Leu Gly Glu Gly Ala Ala Ala Lys Gly His Asn725 730 735
Ile Leu Glu Lys Thr Ile Lys Pro Gly Ser Leu Lys Ala Ile Tyr Gly740 745 750Asp Lys Ala Glu Ser Ile Leu Gly Leu Ala Lys Arg Ser Gly Leu Val755 760 765Gly Arg Val Gly Gln Trp Asp Ala Ser Gly Val Arg Gly Ile Tyr Ala770 775 780His Asn Arg Pro Gly Gly Glu Asp Leu Val Tyr Pro Val Ser Leu Gln785 790 795 800Asn Thr Ser Ala Asn Glu Ile Val Asn Ala Trp Ile Lys Phe Lys Ile805 810 815Ile Thr Pro Tyr Thr Gly Asp Tyr Asp Met His Asp Ile Ile Lys Phe820 825 830Ser Asp Gly Lys Gly His Val Pro Thr Ala Glu Ser Ser Glu Glu Arg835 840 845Gly Val Lys Asp Leu Ile Asn Lys Gly Val Ala Glu Val Asp Pro Ser850 855 860Arg Pro Phe Glu Tyr Thr Ala Met Asn Val Ile Arg His Gly Pro Gln865 870 875 880Val Asn Phe Val Pro Tyr Met Trp Glu His Glu His Asp Lys Val Val885 890 895Asn Asp Asn Gly Tyr Leu Gly Val Val Ala Ser Pro Gly Pro Phe Pro900 905 910
Val Ala Met Val His Gln Gly Glu Trp Thr Val Phe Asp Asn Ser Glu915 920 925Glu Leu Phe Asn Phe Tyr Lys Ser Thr Asn Thr Pro Leu Pro Glu His930 935 940Trp Ser Gln Asp Phe Met Asp Arg Gly Lys Gly Ile Val Ala Thr Pro945 950 955 960Arg His Ala Glu Leu Leu Asp Lys Arg Arg Val Met Tyr965 970<210>62<211>2499<212>PRT<213>发光光杆状菌<400>62Met Asn Thr Leu Lys Ser Glu Tyr Gln Gln Ala Leu Gly Ala Gly Phe1 5 10 15Asn Asn Leu Thr Asp Ile Cys His Leu Ser Phe Asp Glu Leu Arg Lys20 25 30Lys Val Lys Asp Lys Leu Ser Trp Ser Gln Thr Gln Ser Leu Tyr Leu35 40 45Glu Ala Gln Gln Val Gln Lys Asp Asn Leu Leu His Glu Ala Arg Ile50 55 60Leu Lys Arg Ala Asn Pro His Leu Gln Ser Ala Val His Leu Ala Leu65 70 75 80
Thr Ala Pro His Ala Asp Gln Gln Gly Tyr Asn Ser Arg Phe Gly Asn85 90 95Arg Ala Ser Lys Tyr Ala Ala Pro Gly Ala Ile Ser Ser Met Phe Ser100 105 110Leu Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Gln Ala Arg Asn Leu His115 120 125Ala Glu Gly Ser Ile Tyr His Leu Asp Thr Arg Arg Pro Asp Leu Lys130 135 140Ser Leu Val Leu Ser Gln Lys Asn Met Asn Thr Glu Ile Ser Thr Leu145 150 155 160Ser Leu Ser Asn Asn Met Leu Leu Asn Ser Ile Lys Thr Gln Pro Asn165 170 175Leu Asn Ser His Ala Lys Val Met Glu Lys Leu Ser Thr Phe Arg Thr180 185 190Ser Gly Ser Met Pro Tyr His Asp Ala Tyr Glu Ser Val Arg Lys Ile195 200 205Ile Gln Leu Gln Ala Pro Val Phe Glu Gln Ser Ser Thr Leu Thr Asp210 215 220Thr Pro Ile Thr Lys Leu Met Tyr Gln Ile Ser Leu Leu Gly Ile Asn225 230 235 240Ala Ser Val Ser Pro Glu Leu Phe Thr Ile Leu Thr Gln Lys Ile Lys245 250 255
Pro Ala Thr Asn Ala Asp Asn Thr Asn Glu Leu Lys Lys Leu Tyr Lys260 265 270Lys Asn Phe Gly Glu Ile Lys Ser Ile Gln Met Ala Arg Ala Glu Tyr275 280 285Leu Lys Ser Tyr Tyr Asn Leu Thr Asp Lys Glu Leu Asn Gln Phe Ser290 295 300Lys Lys Ile Lys Gln Ile Asp Ser Leu Trp Asn Ile Gly Asp Glu Ile305 310 315 320Thr Gln Tyr His Leu Leu Lys Phe Asn Lys Ala Ile Asn Leu Ser Arg325 330 335Ser Thr Glu Leu Ser Pro Ile Ile Leu Asn Ser Ile Ala Ile Asp Ile340 345 350Leu Lys Lys Thr Pro Pro Glu Asp Asp Ser Asp Asn Pro Phe Arg Asp355 360 365Asp Pro Asp Tyr Leu Glu Ser Phe Gln Asp Leu Asp Leu Ser Asp Glu370 375 380Pro Asp Ile Asp Glu Asp Val Leu Arg Glu Ala Leu Arg Val Lys Asp385 390 395 400Tyr Met Gln Arg Tyr Gly Ile Asp Ala Glu Thr Ala Leu Ile Leu Cys405 410 415Lys Ala Pro Ile Ser Glu Asn Pro Ser His Pro Asp Leu Ser Lys Leu420 425 430
Leu Ala Asp Ile His Gln Leu Thr Ile Asp Glu Leu Gly Val Leu Leu435 440 445Val Ala Ile Asp Glu Gly Lys Thr Asp Leu Ser Gln Ile Thr His Asp450 455 460Asn Leu Ala Val Leu Ile Ser Lys Leu Tyr Ser Val Thr Asn Trp Leu465 470 475 480Arg Thr Arg Lys Trp Ser Val Tyr Gln Leu Phe Val Met Thr Thr Asp485 490 495Lys Tyr Asn Lys Thr Leu Thr Pro Glu Ile Asn Asn Leu Leu Asp Thr500 505 5l0Val Tyr Asn Gly Leu Gln Asn Phe Tyr Lys Asp Asn Leu Leu Lys Ile515 520 525Lys Asp Asn Leu Leu Lys Ala Lys Glu Ser Leu Pro Glu Asp Lys Asp530 535 540Asn Leu Pro Lys Ala Glu Gln Tyr Leu Leu Glu Ala Glu Lys Tyr Leu545 550 555 560Leu Ala Ala Glu Lys Tyr Leu Leu Ala Ala Glu Lys Tyr Leu Leu Glu565 570 575Ala Asn Lys Asn Pro Leu Glu Ala Lys Lys Ala Leu Lys Glu Tyr Glu580 585 590Lys Asn Gln Glu Ala Tyr Glu Lys Asn Leu Lys Glu His Glu Lys Tyr595 600 605
Leu Leu Lys Ala Gly Glu Asn Leu Pro Ala Ile Lys Glu Asn Leu Leu610 615 620Lys Ile Lys Glu Asn Leu Pro Lys Ala Ile Ser Pro Tyr Ile Ala Ala625 630 635 640Ala Leu Gln Leu Pro Ser Glu Asn Val Ala Leu Ser Val Leu Ala Trp645 650 655Ala Asp Lys Leu Asn Ser Gly Lys Glu Asn Lys Met Thr Ala Asp Ser660 665 670Phe Trp Asn Trp Leu Arg Lys Lys Pro Ile Glu Thr Gln Ser Lys Thr675 680 685Thr Glu Ala Thr Glu Ala Thr Glu Ala Thr Glu Ala Thr Glu Ala Thr690 695 700Glu Ala Thr Glu Lys Thr Thr Leu Ile Gln Gln Ala Val Gln Tyr Cys705 710 715 720Gln Cys Leu Ala Gln Leu Ala Leu Ile Tyr Arg Ser Thr Gly Leu Ser725 730 735Glu Ser Thr Leu Arg Leu Phe Val Thr Asn Pro Gln Ile Phe Gly Leu740 745 750Thr Ala Lys Thr Thr Ser Thr His Asn Val Leu Ser Leu Ile Met Leu755 760 765Thr Arg Phe Thr Asp Trp Val Asn Ser Leu Gly Glu Asn Ala Ser Ser770 775 780
Val Leu Thr Glu Phe Glu Lys Gly Thr Leu Thr Ala Glu Leu Leu Ala785 790 795 800Asn Ala Met Asn Leu Asp Lys Asn Leu Leu Glu Gln Ala Ser Thr Gln805 810 815Ala Gln Ala Asp Phe Ser Asn Trp Pro Ser Ile Asp Asn Leu Leu Gln820 825 830Trp Ile Asn Ile Ser Arg Gln Leu Asn Ile Ser Pro Gln Gly Val Ser835 840 845Glu Leu Ala Lys Ile Leu Asp Ile Glu Ser Ser Thr Asn Tyr Ala Gln850 855 860Trp Glu Asn Val Ala Ser Ile Leu Thr Ala Gly Leu Asp Thr Gln Lys865 870 875 880Ala Asn Thr Leu His Ala Phe Leu Gly Glu Ser Arg Ser Thr Ala Leu885 890 895Ser Thr Tyr Tyr Ile Tyr Ser His Asn Gln Lys Asp Arg Glu Glu Arg900 905 910Lys His Thr Val Ile Lys Asp Arg Asp Asp Leu Tyr Gln Tyr Leu Leu915 920 925Ile Asp Asn Gln Val Ser Ala Ala Ile Lys Thr Thr Glu Ile Ala Glu930 935 940Ala Ile Ala Ser Ile Gln Leu Tyr Ile Asn Arg Ala Leu Lys Asn Met945 950 955 960
Glu Gly Asp Thr Asp Thr Ser Val Thr Ser Arg Leu Phe Phe Thr Asn965 970 975Trp Asp Lys Tyr Asn Lys Arg Tyr Ser Thr Trp Ala Gly Ile Thr Lys980 985 990Leu Leu Tyr Tyr Pro Glu Asn Tyr Ile Asp Pro Thr Leu Arg Ile Gly995 10001005Gln Thr Lys Met Met Asp Thr Leu Leu Gln Ser Ile Ser Gln Ser1010 1015 1020Gln Leu Asn Thr Asp Thr Val Glu Asp Ala Phe Lys Ser Tyr Leu1025 1030 1035Thr Ser Phe Glu Gln Val Ala Asn Leu Glu Val Ile Ser Ala Tyr1040 1045 1050His Asp Asn Ile Asn Asn Asp Gln Gly Leu Thr Tyr Phe Ile Gly1055 1060 1065Arg Ser Lys Thr Glu Val Asn Gln Tyr Tyr Trp Arg Ser Val Asp1070 1075 1080His Asn Lys Phe Ser Glu Gly Lys Phe Pro Ala Asn Ala Trp Ser1085 1090 1095Glu Trp His Lys Ile Asp Cys Pro Ile Asn Pro Tyr Glu Asp Thr1100 1105 1110Ile Arg Pro Val Val Tyr Gln Ser Arg Leu Tyr Ile Ile Trp Leu1115 1120 1125
Glu Gln Lys Lys Val Thr Asn Arg Ala Glu Gly Glu Ala Ile Lys1130 1135 1140Gln Gly Ser Lys Thr Thr Thr Ser Tyr His Tyr Glu Leu Lys Leu1145 1150 1155Ala His Ile Arg Tyr Asp Gly Thr Trp Asn Thr Pro Ile Thr Phe1160 1165 1170Asp Val Asp Glu Lys Ile Ser Gly Leu Asn Leu Glu Leu Asn Lys1175 1180 1185Ala Leu Gly Leu Tyr Cys Ala Ser Tyr Gln Gly Lys Asp Lys Leu1190 1195 1200Leu Val Met Phe Tyr Lys Lys Gln Glu Gln Leu Asn Asn Tyr Thr1205 1210 1215Glu Lys Thr Gly Asn Thr Tyr Thr Ala Pro Ile Lys Gly Leu Tyr1220 1225 1230Ile Thr Ser Asn Met Ser Pro Glu Glu Met Thr Pro Glu Ser Tyr1235 1240 1245Arg Leu Asn Ala His Lys Gln Phe Asp Thr Asn Asn Val Val Arg1250 1255 1260Val Asn Asn Arg Tyr Ala Glu Ser Tyr Glu Ile Pro Ser Ser Val1265 1270 1275Asn Ser Asn Asn Gly Tyr Asp Trp Gly Glu Gly Tyr Leu Ser Met1280 1285 1290
Val Tyr Gly Gly Ser Ile Leu Ile Thr Arg Asp Pro Ser Asp Asn1295 1300 1305Ser Lys Ile Gln Ile Ser Pro Lys Leu Arg Ile Ile His Asn Gly1310 1315 1320Tyr Glu Gly Arg Gln Arg Asn Gln Cys Asn Leu Met Lys Lys Tyr1325 1330 1335Gly Lys Leu Gly Asp Lys Phe Ile Ile Tyr Thr Thr Leu Gly Ile1340 1345 1350Asn Pro Asn Asn Leu Ser Asn Lys Lys Leu Ile Tyr Pro Val Tyr1355 1360 1365Gln Tyr Glu Gly Asn Glu Ser Lys Leu Ser Gln Gly Arg Leu Leu1370 1375 1380Phe Tyr Arg Asp Ser Thr Thr Asn Phe Thr Arg Ala Trp Phe Pro1385 1390 1395Asn Leu Ser Ser Asp Ser Lys Glu Met Ser Ile Thr Thr Gly Gly1400 1405 1410Asn Ile Ser Gly Asn Tyr Gly Tyr Ile Asp Asn Lys His Ser Asp1415 1420 1425Asn Lys Pro Phe Glu Glu Tyr Phe Tyr Met Asp Asp His Gly Gly1430 1435 1440Ile Asp Thr Asp Val Ser Glu Pro Ile Phe Ile Asn Thr Lys Ile1445 1450 1455
Gln Pro Ser Asn Val Lys Ile Ile Val Lys Thr Val Lys Asp Asp1460 1465 1470Gly Lys Leu Asp Ser Lys Pro Tyr Ile Ala Glu Asp Lys Val Ser1475 1480 1485Val Lys Pro Thr Pro Asn Phe Glu Glu Met Cys Tyr Gln Phe Asn1490 1495 1500Asn Leu Asp Gln Ile Asp Val Ser Thr Leu Val Phe Lys Asn Asn1505 1510 1515Glu Ala Ser Ile Asp Ile Thr Phe Thr Ala Ser Ala Asp Ala Phe1520 1525 1530Glu Ser Gly Lys Glu Gln Arg Asn Leu Gly Glu Glu His Phe Ser1535 1540 1545Ile Arg Ile Ile Lys Lys Ala Asn Val Asn Asp Val Leu Thr Leu1550 1555 1560His His Asp Pro Ser Gly Ala Gln Tyr Met Gln Trp Gly Ala Tyr1565 1570 1575Arg Thr Arg Leu Asn Thr Leu Phe Ala Arg Lys Leu Ile Ser Arg1580 1585 1590Ala Asn Ala Gly Ile Asp Thr Ile Leu Ser Met Glu Thr Gln Asn1595 1600 1605Ile Gln Glu Pro Gln Leu Gly Lys Gly Phe Tyr Val Asn Phe Thr1610 1615 1620
Leu Pro Lys Tyr Asp Gln Asn Thr His Gly Asn Glu Arg Gln Phe1625 1630 1635Lys Ile His Ile Gly Asn Ile Ala Gly Asp Asn Thr Met Arg Pro1640 1645 1650Tyr Tyr Gln Gly Ile Leu Ala Asp Thr Glu Thr Ser Val Val Leu1655 1660 1665Phe Val Pro Tyr Glu Lys Gln Ser Tyr Thr Asn Glu Gly Val Arg1670 1675 1680Leu Gly Val Glu Tyr Lys Lys Val Ser Tyr Leu Gly Val Trp Glu1685 1690 1695Pro Ala Phe Phe Tyr Phe Asn Glu Ile Gln Gln Lys Phe Ile Leu1700 1705 1710Ile Asn Asp Ala Asp His Asn Ser Ala Met Thr Gln Ser Gly Glu1715 1720 1725Lys Thr Gly Ile Lys Lys Tyr Lys Gly Phe Leu Asp Val Ser Ile1730 1735 1740Leu Ile Asp His Gln His Thr Glu Pro Met Asp Phe Asn Gly Ala1745 1750 1755Asn Ser Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro Met Leu1760 1765 1770Ile Ala Gln Arg Leu Leu His Glu Gln Asn Phe Asp Glu Ala Asn1775 1780 1785
Arg Trp Leu Lys Tyr Val Trp Asn Pro Ser Gly His Ile Ala Asn1790 1795 1800Gly Gln Lys Gln His Pro His Asn Trp Asn Val Arg Pro Leu Gln1805 1810 1815Glu Asp Thr Ser Trp Asn Asp Asp Pro Leu Asp Thr Phe Asp Pro1820 1825 1830Asp Ala Ile Ala Gln His Asp Pro Met His Tyr Lys Val Ala Thr1835 1840 1845Phe Met Cys Ala Leu Asp Leu Leu Ile Glu Gln Gly Asp Tyr Ala1850 1855 1860Tyr Arg Gln Leu Glu Arg Asp Thr Leu Ala Glu Ala Lys Met Trp1865 1870 1875Tyr Met Gln Ala Leu His Leu Leu Gly Asp Lys Pro His Leu Leu1880 1885 1890Leu Ser Ser Thr Trp Ser Asp Pro Glu Leu Lys Glu Ala Ala Asp1895 1900 1905Leu Glu Lys Gln Gln Ala His Ala Lys Ala Ile Ala Asp Leu Arg1910 1915 1920Gln Gly Gln Pro Lys Asp Gly Ser Asn Thr Asp Leu Phe Leu Pro1925 1930 1935Gln Val Asn Glu Val Met Leu Ser Tyr Trp Gln Lys Leu Glu Gln1940 1945 1950
Arg Leu Tyr Asn Leu Arg His Asn Leu Ser Ile Asp Gly Gln Pro1955 1960 1965Leu His Leu Pro Ile Phe Ala Thr Pro Ala Asp Pro Lys Ala Leu1970 1975 1980Leu Ser Ala Ala Val Ala Ser Ser Gln Gly Gly Ser Asn Leu Pro1985 1990 1995Ser Glu Phe Ile Ser Val Trp Arg Phe Pro His Met Leu Glu Asn2000 2005 2010Ala Arg Ser Met Val Ser Gln Leu Thr Gln Phe Gly Ser Thr Leu2015 2020 2025Gln Asn Ile Ile Glu Arg Gln Asp Ala Glu Ala Leu Asn Thr Leu2030 2035 2040Leu Gln Asn Gln Ala Ala Glu Leu Ile Leu Thr Asn Leu Ser Ile2045 2050 2055Gln Asp Lys Thr Ile Glu Glu Leu Asp Val Glu Lys Thr Val Leu2060 2065 2070Glu Lys Thr Arg Ala Gly Ala Lys Ser Arg Phe Asp Ser Tyr Ser2075 2080 2085Lys Phe Tyr Asp Glu Asp Ile Asn Ala Gly Glu Lys Gln Ala Met2090 2095 2100Ala Leu Arg Ala Ser Val Ala Gly Ile Ser Thr Ala Leu Gln Ala2105 2110 2115
Ser His Leu Ala Gly Ala Ala Leu Asp Leu Ala Pro Asn Ile Phe2120 2125 2130Gly Phe Ala Asp Gly Gly Ser His Trp Gly Ala Ile Ala Gln Ala2135 2140 2145Thr Ser Asn Val Met Glu Phe Ser Ala Ser Val Met Ser Thr Glu2150 2155 2160Ala Asp Lys Ile Ser Gln Ser Glu Ala Tyr Arg Arg Arg Arg Gln2165 2170 2175Glu Trp Lys Ile Gln Arg Asn Asn Ala Asp Ala Glu Leu Lys Gln2180 2185 2190Ile Asp Ala Gln Leu Gln Ser Leu Val Val Arg Arg Glu Ala Ala2195 2200 2205Val Leu Gln Lys Thr Ser Leu Lys Thr Gln Gln Glu Gln Thr His2210 2215 2220Ala Gln Leu Thr Phe Leu Gln His Lys Phe Ser Asn Gln Ala Leu2225 2230 2235Tyr Asn Trp Leu Arg Gly Arg Leu Ser Ala Ile Tyr Phe Gln Phe2240 2245 2250Tyr Asp Leu Ala Val Ala Arg Cys Leu Met Ala Glu Met Ala Tyr2255 2260 2265Arg Trp Glu Thr Asn Asp Ala Ala Ala Arg Phe Ile Lys Pro Gly2270 2275 2280
Ala Trp Gln Gly Thr His Ala Gly Leu Leu Ala Gly Glu Thr Leu2285 2290 2295Met Leu Asn Leu Ala Gln Met Glu Asp Ala His Leu Lys Gln Glu2300 2305 2310Gln Arg Val Leu Glu Val Glu Arg Thr Val Ser Leu Ala Glu Val2315 2320 2325Tyr Lys Glu Lys Gly Gln Phe Ser Leu Thr Lys Lys Ile Ala Glu2330 2335 2340Leu Val Asn Lys Lys Pro Asp Thr Thr Ser Ser Arg Asn Asn Thr2345 2350 2355Leu Asn Phe Gly Glu Gly Asn Ala Lys Thr Ser Leu Gln Ala Ser2360 2365 2370Ile Ser Leu Ala Asp Leu Gln Ile Arg His Asp Tyr Pro Glu Asn2375 2380 2385Ser Gly Ala Gly Asn Val Arg Arg Ile Lys Gln Ile Ser Val Thr2390 2395 2400Leu Pro Ala Leu Leu Gly Pro Tyr Gln Asp Val Gln Ala Ile Leu2405 2410 2415Ser Tyr Gly Gly Asp Ala Thr Gly Leu Ala Lys Gly Cys Lys Ala2420 2425 2430Leu Ala Val Ser His Gly Met Asn Asp Ser Gly Gln Phe Gln Leu2435 2440 2445
Asp Phe Asn Asp Gly Lys Phe Leu Pro Phe Glu Gly Ile Glu Ile2450 2455 2460Asp Lys Gly Thr Leu Thr Leu Ser Phe Pro Asn Ala Thr Glu Lys2465 2470 2475Gln Lys Thr Met Leu Glu Ser Ile Ser Asp Ile Ile Leu His Ile2480 2485 2490Arg Tyr Thr Ile Arg Gln2495<210>63<211>2381<212>PRT<213>发光光杆状菌<400>63Met Asn Ser Tyr Val Lys Glu Ile Pro Asp Val Leu Gln Ser Gln Tyr1 5 10 15Gly Ile Asn Cys Leu Thr Asp Ile Cys His Tyr Ser Phe Asn Glu Phe20 25 30Arg Gln Gln Val Ser Asp His Leu Ser Trp Ser Glu Thr Asn Arg Leu35 40 45Tyr Arg Asp Ala Gln Gln Glu Gln Lys Glu Asn Gln Leu Tyr Glu Ala50 55 60Arg Ile Leu Lys Arg Ala Asn Pro Gln Leu Gln Asn Ala Val His Leu65 70 75 80
Gly Ile Thr Leu Pro His Ala Glu Leu Arg Gly Tyr Asn Ser Glu Phe85 90 95Gly Gly Arg Ala Ser Gln Tyr Val Ala Pro Gly Ser Val Ser Ser Met100 105 110Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Arg Asn115 120 125Leu His Ala Ser Asp Ser Val Tyr His Leu Asp Glu Arg Arg Pro Asp130 135 140Leu Gln Ser Met Thr Leu Ser Gln Gln Asn Met Asp Thr Glu Leu Ser145 150 155 160Thr Leu Ser Leu Ser Asn Glu Ile Leu Leu Lys Gly Ile Lys Ala Asn165 170 175Gln Ser Asn Leu Asp Ser Asp Thr Lys Val Met Glu Met Leu Ser Thr180 185 190Phe Arg Pro Ser Gly Thr Ile Pro Tyr His Asp Ala Tyr Glu Asn Val195 200 205Arg Lys Ala Ile Gln Leu Gln Asp Pro Lys Leu Glu Gln Phe Gln Lys210 215 220Ser Pro Ala Val Ala Gly Leu Met His Gln Ala Ser Leu Leu Gly Ile225 230 235 240Asn Asn Ser Ile Ser Pro Glu Leu Phe Asn Ile Leu Thr Glu Glu Ile245 250 255
Thr Glu Ala Asn Ala Glu Ala Ile Tyr Lys Gln Asn Phe Gly Asp Ile260 265 270Asp Pro Ala Cys Leu Ala Met Pro Glu Tyr Leu Lys Ser Tyr Tyr Asn275 280 285Phe Ser Asp Glu Glu Leu Ser Gln Phe Ile Arg Lys Tyr Pro Asp Asn290 295 300Glu Leu Asn Thr Gln Lys Ile His Leu Leu Lys Ile Asn Lys Ile Ile305 310 315 320Leu Leu Ser Gln Ala Val Asn Leu Pro Phe Leu Lys Leu Asp Glu Ile325 330 335Ile Pro Glu Gln Asn Ile Thr Pro Thr Val Leu Gly Lys Ile Phe Leu340 345 350Val Lys Tyr Tyr Met Gln Lys Tyr Asn Ile Gly Thr Glu Thr Ala Leu355 360 365Ile Leu Cys Asn Asp Ser Ile Ser Gln Tyr Ser Tyr Ser Asn Gln Pro370 375 380Ser Gln Phe Asp Arg Leu Phe Asn Thr Ser Pro Leu Asn Gly Gln Tyr385 390 395 400Phe Val Ile Glu Asp Thr Asn Ile Asp Leu Ser Leu Asn Ser Thr Asp405 410 415Asn Trp His Lys Ala Val Leu Lys Arg Ala Phe Asn Val Asp Asp Ile420 425 430
Ser Leu Tyr Arg Leu Leu His Ile Ala Asn His Asn Asn Thr Asp Gly435 440 445Lys Ile Ala Asn Asn Ile Lys Asn Leu Ser Asn Leu Tyr Met Thr Lys450 455 460Leu Leu Ala Asp Ile His Gln Leu Thr Ile Asp Glu Leu Tyr Leu Leu465 470 475 480Leu Ile Thr Ile Gly Glu Asp Lys Ile Asn Leu Tyr Asp Ile Asp Asp485 490 495Lys Glu Leu Glu Lys Leu Ile Asn Arg Leu Asp Thr Leu Ser Asn Trp500 505 510Leu His Thr Gln Lys Trp Ser Ile Tyr Gln Leu Phe Leu Met Thr Thr515 520 525Thr Asn Tyr Asp Lys Thr Leu Thr Pro Glu Ile Gln Asn Leu Leu Asp530 535 540Thr Val Tyr Asn Gly Leu Gln Asn Phe Asp Lys Asn Lys Thr Lys Leu545 550 555 560Leu Ala Ala Ile Ala Pro Tyr Ile Ala Ala Thr Leu Gln Leu Pro Ser565 570 575Glu Asn Val Ala His Ser Ile Leu Leu Trp Ala Asp Lys Ile Lys Pro580 585 590Ser Glu Asn Lys Ile Thr Ala Glu Lys Phe Trp Ile Trp Leu Gln Asn595 600 605
Arg Asp Thr Thr Glu Leu Ser Lys Pro Pro Glu Met Gln Glu Gln Ile610 615 620Ile Gln Tyr Cys His Cys Leu Ala Gln Leu Thr Met Ile Tyr Arg Ser625 630 635 640Ser Gly Ile Asn Glu Asn Ala Phe Arg Leu Phe Ile Glu Lys Pro Thr645 650 655Ile Phe Gly Ile Pro Asp Glu Pro Asn Lys Ala Thr Pro Ala His Asn660 665 670Ala Pro Thr Leu Ile Ile Leu Thr Arg Phe Ala Asn Trp Val Asn Ser675 680 685Leu Gly Glu Lys Ala Ser Pro Ile Leu Thr Ala Phe Glu Asn Lys Thr690 695 700Leu Thr Ala Glu Lys Leu Ala Asn Ala Met Asn Leu Asp Ala Asn Leu705 710 715 720Leu Glu Gln Ala Ser Ile Gln Ala Gln Asn Tyr Lys Gln Val Thr Lys725 730 735Glu Asn Thr Phe Ser Asn Trp Gln Ser Ile Asp Ile Ile Leu Gln Trp740 745 750Thr Asn Ile Ala Ser Asn Leu Asn Ile Ser Pro Gln Gly Ile Ser Pro755 760 765Leu Ile Ala Leu Asp Tyr Ile Lys Pro Ala Gln Lys Thr Pro Thr Tyr770 775 780
Ala Gln Trp Glu Asn Ala Ala Ile Ala Leu Thr Ala Gly Leu Asp Thr785 790 795 800Gln Gln Thr His Thr Leu His Val Phe Leu Asp Glu Ser Arg Ser Thr805 810 815Ala Leu Ser Asn Tyr Tyr Ile Gly Lys Val Ala Asn Arg Ala Ala Ser820 825 830Ile Lys Ser Arg Asp Asp Leu Tyr Gln Tyr Leu Leu Ile Asp Asn Gln835 840 845Val Ser Ala Glu Ile Lys Thr Thr Arg Ile Ala Glu Ala Ile Ala Ser850 855 860Ile Gln Leu Tyr Val Asn Arg Ala Leu Glu Asn Ile Glu Ile His Ala865 870 875 880Val Ser Asp Val Ile Thr Arg Gln Phe Phe Ile Asp Trp Asp Lys Tyr885 890 895Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Gln Leu Val Tyr Tyr900 905 910Pro Glu Asn Tyr Ile Asp Pro Thr Met Arg Ile Gly Gln Thr Lys Met915 920 925Met Asp Thr Leu Leu Gln Ser Val Ser Gln Ser Gln Leu Asn Ala Asp930 935 940Thr Val Glu Asp Ala Phe Lys Ser Tyr Leu Thr Ser Phe Glu Gln Val945 950 955 960
Ala Asn Leu Glu Val Ile Ser Ala Tyr His Asp Asn Val Asn Asn Asp965 970 975Gln Gly Leu Thr Tyr Phe Ile Gly Asn Ser Lys Thr Glu Val Asn Gln980 985 990Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Phe Asn Asp Gly Lys Phe995 10001005Ala Ala Asn Ala Trp Ser Glu Trp His Lys Ile Asp Cys Ala Ile1010 1015 1020Asn Pro Tyr Gln Ser Thr Ile Arg Pro Val Ile Tyr Lys Ser Arg1025 1030 1035Leu Tyr Leu Ile Trp Leu Glu Gln Lys Glu Thr Ala Lys Gln Lys1040 1045 1050Glu Asp Asn Lys Val Thr Thr Asp Tyr His Tyr Glu Leu Lys Leu1055 1060 1065Ala His Ile Arg Tyr Asp Gly Thr Trp Asn Val Pro Ile Thr Phe1070 1075 1080Asp Val Asp Glu Lys Ile Leu Ala Leu Glu Leu Thr Lys Ser Gln1085 1090 1095Ala Pro Gly Leu Tyr Cys Ala Gly Tyr Gln Gly Glu Asp Thr Leu1100 1105 1110Leu Ile Met Phe Tyr Arg Lys Lys Glu Lys Leu Asp Asp Tyr Lys1115 1120 1125
Thr Ala Pro Met Gln Gly Phe Tyr Ile Phe Ser Asp Met Ser Ser1130 1135 1140Lys Asp Met Thr Asn Glu Gln Cys Asn Ser Tyr Arg Asp Asn Gly1145 1150 1155Tyr Thr His Phe Asp Thr Asn Ser Asp Thr Asn Ser Val Ile Arg1160 1165 1170Ile Asn Asn Arg Tyr Ala Glu Asp Tyr Glu Ile Pro Ser Leu Ile1175 1180 1185Asn His Ser Asn Ser His Asp Trp Gly Glu Tyr Asn Leu Ser Gln1190 1195 1200Val Tyr Gly Gly Asn Ile Val Ile Asn Tyr Lys Val Thr Ser Asn1205 1210 1215Asp Leu Lys Ile Tyr Ile Ser Pro Lys Leu Arg Ile Ile His Asp1220 1225 1230Gly Lys Glu Gly Arg Glu Arg Ile Gln Ser Asn Leu Ile Lys Lys1235 1240 1245Tyr Gly Lys Leu Gly Asp Lys Phe Ile Ile Tyr Thr Ser Leu Gly1250 1255 1260Ile Asn Pro Asn Asn Ser Ser Asn Arg Phe Met Phe Tyr Pro Val1265 1270 1275Tyr Gln Tyr Asn Gly Asn Thr Ser Gly Leu Ala Gln Gly Arg Leu1280 1285 1290
Leu Phe His Arg Asp Thr Ser Tyr Ser Ser Lys Val Ala Ala Trp1295 1300 1305Ile Pro Gly Ala Gly Arg Ser Leu Ile Asn Glu Asn Ala Asn Ile1310 1315 1320Gly Asp Asp Cys Ala Glu Asp Ser Val Asn Lys Pro Asp Asp Leu1325 1330 1335Lys Gln Tyr Ile Tyr Met Thr Asp Ser Lys Gly Thr Ala Thr Asp1340 1345 1350Val Ser Gly Pro Val Asp Ile Asn Thr Ala Ile Ser Ser Glu Lys1355 1360 1365Val Gln Ile Thr Ile Lys Ala Gly Lys Glu Tyr Ser Leu Thr Ala1370 1375 1380Asn Lys Asp Val Ser Val Gln Pro Ser Pro Ser Phe Glu Glu Met1385 1390 1395Cys Tyr Gln Phe Asn Ala Leu Glu Ile Asp Gly Ser Asn Leu Asn1400 1405 1410Phe Thr Asn Asn Ser Ala Ser Ile Asp Val Thr Phe Thr Ala Leu1415 1420 1425Ala Asp Asp Gly Arg Lys Leu Gly Tyr Glu Ile Phe Asn Ile Pro1430 1435 1440Val Ile Gln Lys Val Lys Thr Asp Asn Ala Leu Thr Leu Phe His1445 1450 1455
Asp Glu Asn Gly Ala Gln Tyr Met Gln Trp Gly Ala Tyr Arg Ile1460 1465 1470Arg Leu Asn Thr Leu Phe Ala Arg Gln Leu Val Glu Arg Ala Asn1475 1480 1485Thr Gly Ile Asp Thr Ile Leu Ser Met Glu Thr Gln Asn Ile Gln1490 1495 1500Glu Pro Met Met Gly Ile Gly Ala Tyr Ile Glu Leu Ile Leu Asp1505 1510 1515Lys Tyr Asn Pro Asp Ile His Gly Thr Asn Lys Ser Phe Lys Ile1520 1525 1530Ile Tyr Gly Asp Ile Phe Lys Ala Gly Asp His Phe Pro Ile Tyr1535 1540 1545Gln Gly Ala Leu Ser Asp Ile Thr Gln Thr Thr Val Lys Leu Phe1550 1555 1560Leu Pro Arg Val Asp Asn Ala Tyr Gly Asn Lys Asn Asn Leu Tyr1565 1570 1575Val Tyr Ala Ala Tyr Gln Lys Val Glu Thr Asn Phe Ile Arg Phe1580 1585 1590Val Lys Glu Asp Asn Asn Lys Pro Ala Thr Phe Asp Thr Thr Tyr1595 1600 1605Lys Asn Gly Thr Phe Pro Gly Leu Ala Ser Ala Arg Val Ile Gln1610 1615 1620
Thr Val Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ser Leu Tyr1625 1630 1635Phe Trp Glu Leu Phe Tyr Tyr Thr Pro Met Met Val Ala Gln Arg1640 1645 1650Leu Leu His Glu Gln Asn Phe Asp Glu Ala Asn Arg Trp Leu Lys1655 1660 1665Tyr Val Trp Ser Pro Ser Gly Tyr Ile Val Arg Gly Gln Ile Lys1670 1675 1680Asn Tyr His Trp Asn Val Arg Pro Leu Leu Glu Asn Thr Ser Trp1685 1690 1695Asn Ser Asp Pro Leu Asp Ser Val Asp Pro Asp Ala Val Ala Gln1700 1705 1710His Asp Pro Met His Tyr Lys Val Ala Thr Phe Met Arg Thr Leu1715 1720 1725Asp Leu Leu Met Ala Arg Gly Asp His Ala Tyr Arg Gln Leu Glu1730 1735 1740Arg Asp Thr Leu Asn Glu Ala Lys Met Trp Tyr Met Gln Ala Leu1745 1750 1755His Leu Leu Gly Asn Lys Pro Tyr Leu Pro Leu Ser Ser Val Trp1760 1765 1770Asn Asp Pro Arg Leu Asp Asn Ala Ala Ala Thr Thr Thr Gln Lys1775 1780 1785
Ala His Ala Tyr Ala Ile Thr Ser Leu Arg Gln Gly Thr Gln Thr1790 1795 1800Pro Ala Leu Leu Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe1805 1810 1815Leu Pro Gln Ile Asn Asp Val Met Leu Ser Tyr Trp Asn Lys Leu1820 1825 1830Glu Leu Arg Leu Tyr Asn Leu Arg His Asn Leu Ser Ile Asp Gly1835 1840 1845Gln Pro Leu His Leu Pro Ile Tyr Ala Thr Pro Ala Asp Pro Lys1850 1855 1860Ala Leu Leu Ser Ala Ala Val Ala Thr Ser Gln Gly Gly Gly Lys1865 1870 1875Leu Pro Glu Ser Phe Ile Ser Leu Trp Arg Phe Pro His Met Leu1880 1885 1890Glu Asn Ala Arg Ser Met Val Thr Gln Leu Ile Gln Phe Gly Ser1895 1900 1905Thr Leu Gln Asn Ile Ile Glu Arg Gln Asp Ala Glu Ser Leu Asn1910 1915 1920Ala Leu Leu Gln Asn Gln Ala Lys Glu Leu Ile Leu Thr Thr Leu1925 1930 1935Ser Ile Gln Asp Lys Thr Ile Glu Glu Ile Asp Ala Glu Lys Thr1940 1945 1950
Val Leu Glu Lys Ser Lys Ala Gly Ala Lys Ser Arg Phe Asp Asn1955 1960 1965Tyr Ser Lys Leu Tyr Asp Glu Asp Val Asn Ala Gly Glu Arg Gln1970 1975 1980Ala Leu Asp Met Arg Ile Ala Ser Gln Ser Ile Thr Ser Gly Leu1985 1990 1995Lys Gly Leu His Met Ala Ala Ala Ala Leu Glu Met Val Pro Asn2000 2005 2010Ile Tyr Gly Phe Ala Val Gly Gly Thr Arg Tyr Gly Ala Ile Ala2015 2020 2025Asn Ala Ile Ala Ile Gly Gly Gly Ile Ala Ala Glu Gly Leu Leu2030 2035 2040Ile Glu Ala Glu Lys Val Ser Gln Ser Glu Ile Trp Arg Arg Arg2045 2050 2055Arg Gln Glu Trp Glu Ile Gln Arg Asn Asn Ala Glu Ala Glu Met2060 2065 2070Lys Gln Ile Asp Ala Gln Leu Lys Ser Leu Thr Val Arg Arg Glu2075 2080 2085Ala Ala Val Leu Gln Lys Thr Gly Leu Lys Thr Gln Gln Glu Gln2090 2095 2100Thr Gln Ala Gln Leu Ala Phe Leu Gln Arg Lys Phe Ser Asn Gln2105 2110 2115
Ala Leu Tyr Asn Trp Leu Arg Gly Arg Leu Ala Ala Ile Tyr Phe2120 2125 2130Gln Phe Tyr Asp Leu Val Val Ala Arg Cys Leu Met Ala Glu Gln2135 2140 2145Ala Tyr Arg Trp Glu Thr Asn Asp Ser Ser Ala Arg Phe Ile Lys2150 2155 2160Pro Gly Ala Trp Gln Gly Thr Tyr Ala Gly Leu Leu Ala Gly Glu2165 2170 2175Thr Leu Met Leu Asn Leu Ala Gln Met Glu Asp Ala His Leu Lys2180 2185 2190Gln Glu Gln Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu Ala2195 2200 2205Gln Val Tyr Gln Ser Leu Gly Glu Lys Ser Phe Ala Leu Lys Asp2210 2215 2220Lys Ile Glu Ala Leu Leu Gln Gly Asp Lys Glu Thr Ser Ala Gly2225 2230 2235Asn Asp Gly Asn Gln Leu Lys Leu Thr Asn Asn Thr Leu Ser Ala2240 2245 2250Thr Leu Thr Leu Gln Asp Leu Lys Leu Lys Asp Asp Tyr Pro Glu2255 2260 2265Glu Met Gln Leu Gly Lys Thr Arg Arg Ile Lys Gln Ile Ser Val2270 2275 2280
Ser Leu Pro Ala Leu Leu Gly Pro Tyr Gln Asp Val Gln Ala Val2285 2290 2295Leu Ser Tyr Gly Gly Asp Ala Thr Gly Leu Ala Lys Gly Cys Lys2300 2305 2310Ala Leu Ala Val Ser His Gly Leu Asn Asp Asn Gly Gln Phe Gln2315 2320 2325Leu Asp Phe Ash Asp Gly Lys Phe Leu Pro Phe Glu Gly Ile Asp2330 2335 2340Ile Asn Asp Lys Gly Thr Phe Thr Leu Ser Phe Pro Asn Ala Ala2345 2350 2355Ser Lys Gln Lys Asn Ile Leu Gln Met Leu Thr Asp Ile Ile Leu2360 2365 2370His Ile Arg Tyr Thr Ile Leu Glu2375 2380<210>64<211>949<212>PRT<213>发光光杆状菌<400>64Met Lys Asn Ile Asp Pro Lys Leu Tyr Gln His Thr Pro Thr Val Asn1 5 10 15Val Tyr Asp Asn Arg Gly Leu Thr Ile Arg Ash Ile Asp Phe His Arg20 25 30Asp Val Ala Gly Gly Asp Thr Asp Thr Arg Ile Thr Arg His Gln Tyr
35 40 45Asp Thr Arg Gly His Leu Ser Gln Ser Ile Asp Pro Arg Leu Tyr Asp50 55 60Ala Lys Gln Thr Asn Asn Ser Thr Asn Pro Asn Phe Leu Trp Gln Tyr65 70 75 80Asn Leu Thr Gly Asp Thr Leu Arg Thr Glu Ser Val Asp Ala Gly Arg85 90 95Thr Val Ala Leu Asn Asp Ile Glu Gly Arg Gln Val Leu Ile Val Thr100 105 110Ala Thr Gly Ala Ile Gln Thr Arg Gln Tyr Glu Ala Asn Thr Leu Pro115 120 125Gly Arg Leu Leu Ser Val Ser Glu Gln Ala Pro Gly Glu Gln Thr Pro130 135 140Arg Val Thr Glu His Phe Ile Trp Ala Gly Asn Thr Gln Ala Glu Lys145 150 155 160Asp His Asn Leu Ala Gly Gln Tyr Val Arg His Tyr Asp Thr Ala Gly165 170 175Val Thr Gln Leu Glu Ser Leu Ser Leu Thr Glu Asn Ile Leu Ser Gln180 185 190Ser Arg Gln Leu Leu Ala Asp Gly Gln Glu Ala Asp Trp Thr Gly Asn195 200 205Asp Glu Thr Leu Trp Gln Thr Lys Leu Asn Ser Glu Thr Tyr Thr Thr
210 215 220Gln Ser Thr Phe Asp Ala Thr Gly Ala Leu Leu Thr Gln Thr Asp Ala225 230 235 240Lys Gly Asn Met Gln Arg Leu Ala Tyr Asn Val Ala Gly Gln Leu Gln245 250 255Gly Ser Trp Leu Thr Leu Lys Asn Gln Ser Glu Gln Val Ile Val Lys260 265 270Ser Leu Thr Tyr Ser Ala Ala Gly Gln Lys Leu Arg Glu Glu His Gly275 280 285Asn Gly Val Ile Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Leu Arg Leu290 295 300Ile Gly Thr Thr Thr Arg Arg Gln Ser Asp Ser Lys Val Leu Gln Asp305 310 315 320Leu Arg Tyr Glu His Asp Pro Val Gly Asn Ile Ile Ser Val Arg Asn325 330 335Asp Ala Glu Ala Thr Arg Phe Trp Arg Asn Gln Lys Ile Val Pro Glu340 345 350Asn Thr Tyr Thr Tyr Asp Ser Leu Tyr Gln Leu Ile Ser Ala Thr Gly355 360 365Arg Glu Met Ala Asn Ile Gly Gln Gln Ser Asn Gln Leu Pro Ser Pro370 375 380Ile Ile Pro Leu Pro Thr Asp Glu Asn Ser Tyr Thr Asn Tyr Thr Arg
385 390 395 400Ser Tyr Asn Tyr Asp Arg Gly Gly Asn Leu Val Gln Ile Arg His Ser405 410 415Ser Pro Ala Ala Gln Asn Asn Tyr Thr Thr Asp Ile Thr Val Ser Asn420 425 430Arg Ser Asn Arg Ala Val Leu Ser Ser Leu Thr Ser Asp Pro Thr Gln435 440 445Val Glu Ala Leu Phe Asp Ala Gly Gly His Gln Thr Lys Leu Leu Pro450 455 460Gly Gln Glu Leu Ser Trp Asn Thr Arg Gly Glu Leu Lys Gln Val Thr465 470 475 480Pro Val Ser Arg Glu Ser Ala Ser Asp Arg Glu Trp Tyr Arg Tyr Gly485 490 495Asn Asp Gly Met Arg Arg Leu Lys Val Ser Glu Gln Gln Thr Gly Asn500 505 510Ser Thr Gln Gln Gln Arg Val Thr Tyr Leu Pro Asp Leu Glu Leu Arg515 520 525Thr Thr Gln Asn Gly Thr Thr Thr Ser Glu Asp Leu His Ala Ile Thr530 535 540Val Gly Ala Ala Gly His Ala Gln Val Arg Val Leu His Trp Glu Thr545 550 555 560Thr Pro Pro Ala Gly Ile Asn Asn Asn Gln Leu Arg Tyr Ser Tyr Asp
565 570 575Asn Leu Ile Gly Ser Ser Gln Leu Glu Leu Asp Asn Ala Gly Gln Ile580 585 590Ile Ser Gln Glu Glu Tyr Tyr Pro Phe Gly Gly Thr Ala Leu Trp Ala595 600 605Ala Arg Asn Gln Ile Glu Ala Ser Tyr Lys Ile Leu Arg Tyr Ser Gly610 615 620Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg Tyr Tyr625 630 635 640Gln Pro Trp Val Gly Arg Trp Leu Ser Ala Asp Pro Ala Gly Thr Ile645 650 655Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Ser Thr Leu660 665 670Val Asp Ile Ser Gly Leu Ala Pro Thr Lys Tyr Asn Ile Pro Gly Phe675 680 685Asp Phe Asp Val Glu Ile Asp Glu Gln Lys Arg Ser Lys Leu Lys Pro690 695 700Thr Leu Ile Arg Ile Lys Asp Glu Phe Leu His Tyr Gly Pro Val Asp705 710 715 720Lys Leu Leu Glu Glu Lys Lys Pro Gly Leu Asn Val Pro Glu Glu Leu725 730 735Phe Asp Arg Gly Pro Ser Glu Asn Gly Val Ser Thr Leu Thr Phe Lys
740 745 750Lys Asp Leu Pro Ile Ser Cys Ile Ser Asn Thr Glu Tyr Thr Leu Asp755 760 765Ile Leu Tyr Asn Lys His Glu Thr Lys Pro Phe Pro Tyr Glu Asn Glu770 775 780Ala Thr Val Gly Ala Asp Leu Gly Val Ile Met Ser Val Glu Phe Gly785 790 795 800Asn Lys Ser Ile Gly Asn Ala Ser Asp Glu Asp Leu Lys Glu Glu His805 810 815Leu Pro Leu Gly Lys Ser Thr Met Asp Lys Thr Asp Leu Pro Asp Leu820 825 830Lys Gln Gly Leu Met Ile Ala Glu Lys Ile Lys Ser Gly Lys Gly Ala835 840 845Tyr Pro Phe His Phe Gly Ala Ala Ile Ala Val Val Tyr Gly Glu Asp850 855 860Lys Lys Val Ala Ala Ser Ile Leu Thr Asp Leu Ser Glu Pro Lys Arg865 870 875 880Asp Glu Gly Glu Tyr Leu Gln Ser Thr Arg Lys Val Ser Ala Met Phe885 890 895Ile Thr Asn Val Asn Glu Phe Arg Gly His Asp Tyr Pro Lys Ser Lys900 905 910Tyr Ser Ile Gly Leu Val Thr Ala Glu Lys Arg Gln Pro Val Ile Ser
915 920 925Lys Lys Arg Ala Asn Pro Glu Glu Ala Pro Ser Ser Ser Arg Asn Lys930 935 940Lys Leu His Val His94权利要求
1.控制或者抑制昆虫的方法，其包括将所述昆虫与有效量的蛋白质A、蛋白质B和蛋白质C相接触，其中(i)每一种所述的蛋白质A、B和C由天然存在的基因编码或者具有不同于天然存在基因编码的产物、仅通过截短或者保守氨基酸改变的氨基酸序列；(ii)所述的蛋白质A是230-290kDa毒素复合体昆虫毒素，其来源于第一分类种，具有独特的杀昆虫活性，并且具有的氨基酸序列与选自SEQID NO14(XptA1Xwi)、SEQ ID NO34(XptA2Xwi)、SEQ ID NO21(TcdA)、SEQ ID NO62(TcdA2)、SEQ ID NO63(TcdA4)和SEQ IDNO59(TcbA)的序列具有至少40％的同一性；(iii)所述的蛋白质B是130-180kDa毒素复合体增效剂，其具有的氨基酸序列与选自SEQ ID NO22(TcdB1)、SEQ ID NO45(TcdB2)、SEQ IDNO56(TcaC)、SEQ ID NO18(XptC1Xwi)、SEQ ID NO49(XptB1xb)、SEQID NO40(PptB1(orf5))和SEQ ID NO60(SepB)的序列具有至少40％的同一性；(iv)所述的蛋白质C是90-120kDa毒素复合体增效剂，其具有的氨基酸序列与选自SEQ ID NO25(TccC1)、SEQ ID NO58(TccC2)、SEQ IDNO47(TccC3)、SEQ ID NO64(TccC4)、SEQ ID NO57(TccC5)、SEQ IDNO16(XptB1Xwi)、SEQ ID NO51(XptC1xb)、SEQ ID NO43(PptC1(orf6长))、SEQ ID NO42(PptC1(orf6短))和SEQ ID NO61(SepC)的序列具有至少35％的同一性；(v)所述的蛋白质B和所述的蛋白质C中至少一种蛋白质是来源于不同于所述第一分类种的第二分类种；(vi)如果所述的蛋白质B来源于所述第二分类种，则所述蛋白质B的氨基酸序列与已知由所述第一分类种产生的任一蛋白质的氨基酸序列的同一性小于75％；和(vii)如果所述的蛋白质C来源于所述第二分类种，则所述蛋白质C的氨基酸序列与已知由所述第一分类种产生的任一蛋白质的氨基酸序列的同一性小于75％；其中如果特定物种的基因组含有编码特定蛋白质的基因，或者特定蛋白质是通过对特定物种基因组中含有的基因所编码蛋白质的氨基酸序列进行截短或者保守氨基酸变化而设计的，则认为特定蛋白质来源于特定物种。
2.权利要求1的方法，其中所述第一和第二分类种是来源于不同的属。
3.权利要求2的方法，其中所述蛋白质A来源于光杆状菌属物种并且所述蛋白质B和蛋白质C中至少一种蛋白质来源于致病杆菌属、类芽孢杆菌属、沙雷氏菌属或假单胞菌属的物种。
4.权利要求2的方法，其中所述蛋白质A来源于致病杆菌属物种并且所述蛋白质B和蛋白质C中至少一种蛋白质来源于光杆状菌属、类芽孢杆菌属、沙雷氏菌属或假单胞菌属的物种。
5.权利要求2的方法，其中所述蛋白质A、蛋白质B和蛋白质C中的至少一种蛋白质来源于致病杆菌属物种并且所述蛋白质A、蛋白质B和蛋白质C中的至少一种蛋白质来源于光杆状菌属物种。
6.权利要求2的方法，其中所述蛋白质A、蛋白质B和蛋白质C中的至少一种蛋白质来源于光杆状菌属物种并且所述蛋白质A、蛋白质B和蛋白质C中的至少一种蛋白质来源于致病杆菌属物种。
7.权利要求1的方法，其中(i)蛋白质A是SEQ ID NO34(XptA2Xwi)或者SEQ ID NO21(TcdA)，(ii)蛋白质B是SEQ ID NO45(TcdB2)、SEQ ID NO40(PptB11529)或者SEQ ID NO49(XptB1Xb)，和(iii)蛋白质C是SEQ ID NO47(TccC3)、SEQ ID NO42(PptC11529-短)、SEQ ID NO43(PptC11529-长)或者SEQ ID NO51(XptC1xb)。
8.权利要求5的方法，其中所述蛋白质A与选自SEQ ID NO14(XptA1Xwi)和SEQ ID NO34(XptA2Xwi)的蛋白质具有至少40％的同一性。
9.权利要求6的方法，其中所述蛋白质A与选自SEQ ID NO21(TcdA)和SEQ ID NO59(TcbA)的蛋白质具有至少40％的同一性。
10.权利要求8的方法，其中蛋白质B与选自SEQ ID NO22、SEQ IDNO40、SEQ ID NO45和SEQ ID NO49的氨基酸序列具有至少40％的同一性，或者蛋白质C与选自SEQ ID NO25、SEQ ID NO42、SEQ IDNO47和SEQ ID NO51的氨基酸序列具有至少35％的同一性。
11.权利要求9的方法，其中蛋白质B与选自SEQ ID NO18和SEQID NO40的氨基酸序列具有至少40％的同一性，或者蛋白质C与选自SEQ.ID NO16(XptB1wi)和SEQ ID NO42的氨基酸序列具有至少35％的同一性。
12.权利要求5的方法，其中所述蛋白质A由在严格条件下与这样的探针杂交的多核苷酸编码，其中所述探针与编码选自SEQ ID NO14(XptA1wi)和SEQ ID NO20(XptA2wi)的氨基酸序列的核酸序列完全互补。
13.权利要求6的方法，其中所述蛋白质A由在严格条件下与这样的探针杂交的多核苷酸编码，其中所述探针与编码SEQ ID NO21(TcdA)所示氨基酸序列的核酸序列完全互补。
14.权利要求8的方法，其中所述蛋白质B由在严格条件下与这样的探针杂交的多核苷酸编码，其中所述探针与编码选自SEQ ID NO22、SEQID NO40、SEQ ID NO45和SEQ ID NO49的氨基酸序列的核酸序列完全互补，或者所述蛋白质C由在严格条件下与这样的探针杂交的多核苷酸编码，其中所述探针与编码选自SEQ ID NO25、SEQ ID NO42、SEQ IDNO47和SEQ ID NO51的氨基酸序列的核酸序列完全互补。
15.权利要求9的方法，其中所述蛋白质B由在严格条件下与这样的探针杂交的多核苷酸编码，其中所述探针与编码选自SEQ ID NO18和SEQ ID NO40的氨基酸序列的核酸序列完全互补，或者所述蛋白质C由在严格条件下与这样的探针杂交的多核苷酸编码，其中所述探针与编码选自SEQ ID NO16(XptB1wi)和SEQ ID NO42的氨基酸序列的核酸序列完全互补。
16.产生蛋白质A、蛋白质B和蛋白质C的转基因植物或者植物细胞，其中(i)每一种所述的蛋白质A、B和C由天然存在的基因编码或者具有不同于天然存在基因编码的产物、仅通过截短或者保守氨基酸改变的氨基酸序列；(ii)所述的蛋白质A是230-290kDa毒素复合体昆虫毒素，其来源于第一分类种，具有独特的杀昆虫活性，并且具有的氨基酸序列与选自SEQID NO14(XptA1Xwi)、SEQ ID NO34(XptA2Xwi)、SEQ ID NO21(TcdA)、SEQ ID NO62(TcdA2)、SEQ ID NO63(TcdA4)和SEQ IDNO59(TcbA)的序列具有至少40％的同一性；(iii)所述的蛋白质B是130-180kDa毒素复合体增效剂，其具有的氨基酸序列与选自SEQ ID NO22(TcdB1)、SEQ ID NO45(TcdB2)、SEQ IDNO56(TcaC)、SEQ ID NO18(XptC1Xwi)、SEQ ID NO49(XptB1xb)、SEQID NO40(PptB1(orf5))和SEQ ID NO60(SepB)的序列具有至少40％的同一性；(iv)所述的蛋白质C是90-120kDa毒素复合体增效剂，其具有的氨基酸序列与选自SEQ ID NO25(TccC1)、SEQ ID NO58(TccC2)、SEQ IDNO47(TccC3)、SEQ ID NO64(TccC4)、SEQ ID NO57(TccC5)、SEQ IDNO16(XptB1Xwi)、SEQ ID NO51(XptC1xb)、SEQ ID NO43(PptC1(orf6长))、SEQ ID NO42(PptC1(orf6短))和SEQ ID NO61(SepC)的序列具有至少35％的同一性；(v)所述的蛋白质B和所述的蛋白质C中至少一种蛋白质是来源于不同于所述第一分类种的第二分类种；(vi)如果所述的蛋白质B来源于所述第二分类种，则所述蛋白质B的氨基酸序列与已知由所述第一分类种产生的任一蛋白质的氨基酸序列的同一性小于75％；和(vii)如果所述的蛋白质C来源于所述第二分类种，则所述蛋白质C的氨基酸序列与已知由所述第一分类种产生的任一蛋白质的氨基酸序列的同一性小于75％；其中如果特定物种的基因组含有编码特定蛋白质的基因，或者符定蛋白质是通过对特定物种基因组中含有的基因所编码蛋白质的氨基酸序列进行截短或者保守氨基酸变化而设计的，则认为特定蛋白质来源于特定物种。
17.权利要求16的转基因植物或者植物细胞，其中(i)蛋白质A是SEQ ID NO34(XptA2Xwi)或者SEQ ID NO21(TcdA)，(ii)蛋白质B是SEQ ID NO45(TcdB2)、SEQ ID NO40(PptB11529)或者SEQ ID NO49(XptB1xb)，和(iii)蛋白质C是SEQ ID NO47(TccC3)、SEQ ID NO42(PptC11529-短)、SEQ ID NO43(PptC11529-长)或者SEQ ID NO51(XptC1xb)。
18.权利要求1的方法，其中将所述昆虫与第二种蛋白质A相接触，该第二种蛋白质A来源于与所述第一分类种不同的分类种。
19.权利要求18的方法，其中将所述昆虫与选自SEQ ID NO14(XptA1Xwi)和SEQ ID NO34(XptA2Xwi)的第一种蛋白质A和选自SEQ IDNO21(TcdA)和SEQ ID NO59(TcbA)的第二种蛋白质A相接触。
20.权利要求18的方法，其中所述第一种蛋白质A是SEQ ID NO14(XptA1Xwi)，所述第二种蛋白质A是SEQ ID NO21(TcdA)，所述蛋白质B是SEQ ID NO45(TcdB2)并且所述蛋白质C是SEQ ID NO47(TccC3)。
全文摘要
本主题发明涉及到惊人的发现，即从致病杆菌属、光杆状菌属和类芽孢杆菌属得到的毒素复合体(TC)蛋白质可以彼此互换使用。在本发明尤其优选的实施方案中，“独特”TC蛋白质(例如来源于光杆状菌属、致病杆菌属或者类芽孢杆菌属)的毒性通过一种或者多种TC蛋白质“增效剂”而增强，该增效剂的来源生物与毒素来源的生物是不同的属。本领域技术人员应该认识到本公开的益处，这扩展了内涵并拓展了现在认识到的单一类型TC蛋白质的应用范围。最重要的好处是本领域技术人员现在能够使用单一一组增效剂来增强独特的致病杆菌属蛋白质毒素以及独特的光杆状菌属蛋白质毒素的活性。这降低了需要通过转基因植物的表达实现对广谱靶害虫有效控制的基因数量和转化事件。文中也公开了异源TC蛋白质的一些优选组合。
文档编号C07K14/24GK1751125SQ200480004601
公开日2006年3月22日 申请日期2004年1月7日 优先权日2003年1月21日
发明者T·D·海伊, A·D·施勒佩尔, S·A·贝文, S·B·宾特里姆, J·C·米切尔, 李泽生, 倪卫汀, 朱宝龙, D·J·默洛, R·C·阿佩尔－比克霍尔德, T·米德 申请人:美国陶氏益农公司