本发明涉及癫痫诊断领域，具体而言，涉及一种辅助癫痫诊断的标志物及其检测试剂盒。
背景技术：
癫痫是慢性反复发作性短暂脑功能失调综合征。以脑部神经元高度同步化异常放电引起反复痫性发作为特征。癫痫是神经系统常见疾病之一，患病率仅次于脑卒中。癫痫的发病率与年龄有关。一般认为1岁以内患病率最高，1～10岁以后逐渐降低。我国癫痫患者男女之比为(1.15-1.7):1。
癫痫病因极其复杂，可分三大类，并存在多种影响发病的因素：
1.特发性癫痫
可疑遗传倾向且无其他明显病因，常在特定年龄段起病，有特征性临床及脑电图表现，诊断较明确。
2.症状性癫痫
中枢神经系统病变影响结构或功能等，如染色体异常、局灶性或弥漫性脑部疾病，以及某些系统性疾病所致。
(1)局限性或弥漫性脑部疾病①先天性异常胚胎发育中各种病因导致脑穿通畸形、小头畸形、先天性脑积水胼胝体缺如及大脑皮质发育不全，围生期胎儿脑损伤等；②获得性脑损伤如脑外伤，颅脑手术后，脑卒中后，颅内感染后，急性酒精中毒；③产伤所致新生儿癫痫发生率约为1％，分娩时合并产伤多伴脑出血或脑缺氧损害，新生儿合并脑先天发育畸形或产伤，癫痫发病率高达25％；④感染包括中枢神经系统细菌、病毒、真菌、寄生虫、螺旋体感染及aids神经系统并发症等；⑤脑血管疾病如脑动静脉畸形、脑梗死和脑出血等；⑥颅内原发性肿瘤如神经胶质瘤、脑膜瘤等；⑦遗传代谢性疾病如结节性硬化、脑-面血管瘤病、苯丙酮酸尿症等；⑧神经系统变性疾病如alzheimer病、pick病等约1/3的患者合并癫痫发作。
(2)系统性疾病①缺氧性脑病如心搏骤停、co中毒窒息、麻醉意外和呼吸衰竭等可引起肌阵挛性发作或全身性大发作；②代谢性脑病如低血糖症，其他代谢及内分泌障碍如高血糖症、低钙血症、低钠血症，以及尿毒症、肝性脑病和甲状腺毒血症等均可导致癫痫发作；③心血管疾病如心脏骤停、高血压脑病等；④热性惊厥热性发作；⑤子痫；⑥中毒如酒精、异烟肼、卡巴唑等药物及铅、铊等重金属中毒。
3.隐源性癫痫
较多见，临床表现提示症状性癫痫，但未找到明确病因，可在特殊年龄段起病，无特定临床和脑电图表现。
癫痫的临床表现有以下：
1.全面强直-阵挛发作(大发作)
系指全身肌肉抽动及意识丧失的发作。以产伤、脑外伤、脑瘤等较常见。强直-阵挛发作可发生在任何年龄，是各种癫痫中最常见的发作类型。其典型发作可分为强直期、阵挛期、恢复期三个临床阶段。发作期间脑电图为典型的爆发性多棘波和棘-慢波综合，每次棘-慢波综合可伴有肌肉跳动。
2.单纯部分发作
是指脑的局部皮质异常放电而引起的与该部位的功能相对应的症状，发作时意识始终存在，包括运动、感觉、自主神经、精神症状及体征。分为四组：①伴运动症状者；②伴躯体感觉或特殊感觉症状者；③伴自主神经症状和体征者；④伴精神症状者。
3.复杂部分发作
习惯上又称精神运动性发作，伴有意识障碍。先兆多在意识丧失前或即将丧失时发生，发作后不能或部分不能回忆发作细节。4.失神发作(小发作)
其典型表现为意识障碍，而不伴先兆或发作后症状。
5.癫痫持续状态
是指单次癫痫发作超过5分钟，或者短期内癫痫频繁发作，以致患者尚未从前一次发作中完全恢复而又有另一次发作。癫痫持续状态是一种需要抢救的急症。
癫痫的治疗可分为控制发作、病因治疗、外科治疗、一般卫生及预防五个方面。其中最重要的是控制发作，目前以药物治疗为主。
临床上根据癫痫发作类型选用抗癫痫药物，一旦找到可以完全控制发作的药物和剂量，就应不间断地应用。一般应于发作完全控制后，如无不良反应再继续服用3～5年，方可考虑逐渐停药。目前多主张用单药治疗，确认单药治疗失败后，方可加用第2种药物。如失神发作或肌阵挛发作无法用单药控制者，可合用乙琥胺和丙戊酸钠，或其一加用苯二氮类。对合并多种发作类型者可以根据发作类型合理联合用药，但以不超过3种药物为宜。
用药宜从小剂量开始，然后逐渐增量，以既能控制发作，又不产生毒性反应的最小有效剂量为宜。换药宜采取先加用新药及后递减旧药的原则。不能骤然停药。
有些器质性脑病的癫痫患者可能需要终身服药；有人主张发病年龄大于30岁者需谨慎停药，因其停药后复发率较高，需长期服药或终身服药。但仍有10％～15％患者难以控制发作，可以采用外科治疗。
在预防方面：
1.预防癫痫病的发生，应详细地进行家系调查，了解患者双亲同胞和近亲中是否有癫痫发作及其发作特点，对能引起智力低下和癫痫的一些严重遗传性疾病，应进行产前诊断或新生儿期过筛检查，以决定终止妊娠或早期进行治疗。防止分娩意外，新生儿产伤是癫痫发病的重要原因之一，避免产伤对预防癫痫有重要意义。
2.对癫痫患者要及时诊断，及早治疗，治疗越早脑损伤越小，复发越少，预后越好。去除或减轻引起癫痫的原发病如颅内占位性疾病、代谢异常、感染等，对反复发作的病例也有重要意义。
3.癫痫是一种慢性疾病，可迁延数年、甚至数十年之久，因而可对患者身体、精神、婚姻以及社会经济地位等，造成严重的不良影响。患者在家庭关系、学校教育和就业等方面的不幸和挫折、文体活动方面的限制等，可使患者产生耻辱感和悲观心理，从而严重影响患者的身心发育，这就要求社会各界对癫痫患者给予理解和支持。
早诊断早治疗是防治癫痫的有效方法，但目前缺乏癫痫诊断相关的标志物。
鉴于此，特提出本发明。
技术实现要素：
本发明的目的在于提供一种用于辅助癫痫诊断的生物标志物，该生物标志物可以用于辅助癫痫的诊断，为癫痫的诊断提供一种新的思路。
本发明的另一目的在于提供上述生物标志物的应用。
本发明的另一目的在于提供一种辅助癫痫诊断的试剂盒。
本发明的另一目的在于提供一种用于辅助癫痫诊断的基因芯片。
本发明是这样实现的：
第一方面，本发明提供了一种辅助癫痫诊断的生物标志物，其为核酸分子，其相对于正常gpr98基因的编码序列具有突变c.a6812g。
gpr98基因编码的蛋白属于g蛋白偶联受体超家族成员，该蛋白包含一个有7个跨膜蛋白受体的结构域，结合钙离子，在中枢神经系统里面表达。该基因突变与usher综合征2型和家族性热性惊厥有关。已报到一些选择性剪切转录本。
进一步地，在本发明的一些实施方案中，所述标志物的碱基序列如seqidno.1所示。
本发明的研究通过对一个癫痫家系患者的成员的gpr98基因的检测测序发现，该家系中患有癫痫的成员的gpr98基因上具有突变c.a6812g，该基因突变导致其对应的gpr98蛋白的第2271位氨基酸由谷氨酰胺(q)变异为精氨酸(r)，为错义突变。该变异不属于多态性位点，在人群中发生频率极低。在hgmd专业版数据库中未见报道。经家系验证分析，受检人(图1中4号成员)之父(图1中3号成员)该位点无变异，受检人之母(图1中2号成员)该位点杂合变异，受检人之姥爷(图1中1号成员)该位点杂合变异。
因此，含有c.a6812g突变的核酸分子即相对于正常gpr98基因的编码序列具有突变c.a6812g的核酸片段，或者含有突变p.q2271r的蛋白均可以作为癫痫诊断的生物标志物，从分子水平上为癫痫的诊断提供一种快速、可靠的检测辅助标记物。
第二方面，本发明提供了一种辅助癫痫诊断的生物标志物，其为蛋白或多肽，其相对于正常gpr98蛋白具有突变p.q2271r。
进一步地，在本发明的一些实施方案中，所述标志物的氨基酸序列如seqidno.2所示。
另一方面，本发明提供了检测第一方面所述的标志物的引物或探针、或检测权第二方面所述的生物标志物的抗体在制备癫痫辅助诊断试剂盒、癫痫产前诊断试剂盒或新生儿癫痫筛查试剂盒中的应用。
基于本发明的研究成果，本领域技术人员应当很容易将用于检测含有c.a6812g突变的gpr98突变基因的引物或探针，以及用于检测含有p.q2271r突变的gpr98突变蛋白的抗体用于制备癫痫辅助诊断试剂盒、癫痫产前诊断试剂盒以及新生儿癫痫筛查试剂盒等领域中。
或者将检测含有c.a6812g突变的gpr98突变基因的探针用于制备辅助癫痫诊断的基因芯片中。
进一步，在本发明的一些实施方案中，所述探针相对于正常gpr98基因的编码序列的第6812位的碱基为g。
另一方面，本发明提供了一种辅助癫痫诊断的试剂盒，其含有用于检测第一方面所述的生物标志物的引物或探针；
或者所述试剂盒含有检测第二方面所述的生物标志物的抗体。
另一方面，本发明提供了一种用于辅助癫痫诊断的基因芯片，该基因芯片上含有用于与目标片段特异性结合的探针，所述目标片段性相对于正常gpr98基因的编码序列具有突变c.a6812g。
所述探针与目标片段特异性结合的区域涵盖如下位置：相对于正常gpr98基因的第6812位。
进一步，在本发明的一些实施方案中，所述探针相对于正常gpr98基因编码序列的第6812位的碱基为g。
进一步，在本发明的一些实施方案中，所述目标片段为来自待测主体的gpr98基因的编码序列。
进一步，在本发明的一些实施方案中，所述主体为人。
附图说明
为了更清楚地说明本发明实施例的技术方案，下面将对实施例中所需要使用的附图作简单地介绍，应当理解，以下附图仅示出了本发明的某些实施例，因此不应被看作是对范围的限定，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他相关的附图。
图1为本发明实施例1中的受检对象癫痫患者家系的遗传家系图谱；
图2-a为本发明实施例1中的遗传家系图谱中的4号成员的gpr98基因测序结果图；
图2-b为本发明实施例1中的遗传家系图谱中的1号成员的gpr98基因测序结果图；
图2-c为本发明实施例1中的遗传家系图谱中的2号成员的gpr98基因测序结果图；
图2-d为本发明实施例1中的遗传家系图谱中的3号成员的gpr98基因测序结果图。
具体实施方式
为使本发明实施例的目的、技术方案和优点更加清楚，下面将对本发明实施例中的技术方案进行清楚、完整地描述。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。
以下结合实施例对本发明的特征和性能作进一步的详细描述。
实施例1
1癫痫患者家系的gpr98基因突变检测
1.1对象：来自重庆市沙坪坝区杨氏癫痫家系先证者及其家系成员，该家系的遗传图谱如图1所示，包括3例癫痫患者(图中124号成员)，1例正常家庭成员(图中3号成员)；1号、2号、4号：全面强直-阵挛发作；3号：未患病。对所有1-4号家庭成员进行详细体格检查，在签署知情同意书后每人采集血液样本。
1.2测序：
将采集的血液样本送北京迈基诺基因科技股份有限公司测序进行癫痫基因突变筛查及验证，分析临床表型、基因型的关系，结果如下：
将测得的序列与正常gpr98基因标准序列比对(https://www.ncbi.nlm.nih.gov/nuccore/nm_032119.3)，测序结果如图2-a所示，先证者即4号成员gpr98基因有1个杂合突变：c.6812a>g(编码区第6812号核苷酸由腺嘌呤变异为鸟嘌呤)，导致氨基酸改变p.q2271r(第2271号氨基酸由谷氨酰胺变异为精氨酸)，为错义突变。该变异不属于多态性位点，在人群中发生频率极低。在hgmd专业版数据库中未见报道。经家系验证分析，先证者(4号成员)之父(图1中3号成员)该位点无变异，3号成员的测序结果如图2-d所示，先证者之母(图1中2号成员)该位点杂合变异，2号成员的测序结果如图2-c所示，先证者之姥爷(图1中1号成员)该位点杂合变异，1号成员的测序结果如图2-b所示。图2的a-d中，箭头所示为gpr98基因6812位点的测序结果，1、2、4号成员在该位点的测序结果为均杂合突变(基因型为ag型)，3号成员在该位点的测序结果为无突变(基因型型为aa型)。
c.6812a>g变异信息如下：
综上分析可以看出，gpr98基因6812位点发生突变a>g与癫痫发生相关，该位点可以作为辅助诊断癫痫疾病的靶点，且含有该突变c.a6812g的核酸片段或相应的具有突变p.q2271r的gpr98蛋白均可以作为辅助诊断癫痫疾病的生物标志物。相应的，用于检测含有具有突变c.a6812g的核酸片段的探针、引物或其他试剂以及检测具有突变p.q2271r的gpr98蛋白的抗体等均可以用于制备辅助诊断癫痫疾病的制剂盒。
实施例2
本实施例提供了检测gpr98基因第6812位点突变的试剂盒，其包括：用于检测gpr98基因6812位点是否发生突变a6812g的引物，正向引物：tccactataggatggtgcttgg，反向引物：agcatagtttgaacattcttgcag；以及pcr扩增试剂：10×buffer(含15mmmg2+)、dntp(2.5mm)、高保真dna聚合酶pfudna聚合酶(5u/μl)以及ddh2o。
使用试剂盒的检测gpr98基因第6812位点是否发生突变a>g的方法，主要包括如下步骤：
(1)提取样本dna，以此为模板，利用上述pcr反应试剂盒进行pcr反应。
(2)多重pcr产物检测：用琼脂糖凝胶对pcr扩增产物进行电泳检测pcr是否成功扩增，扩增片段小461bp。
(3)将pcr产物直接测序，将测序结果与正常gpr98基因序列比对，可判断待测样本的gpr98基因6812位点是否发生突变a>g。
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，本发明可以有各种更改和变化。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
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gaaaggacagttataattgatgaagatagaatatcaagatatgaagaaatcacagtggtt1260
agaaatggaggaacccatgggaatgtctctgcgaattgggtgttgacacggaacagcact1320
gatccctcaccagtaacagcagatatcagaccgagctctggagttctccattttgcacaa1380
gggcagatgttggcaacaattcctcttactgtggttgatgatgatcttccagaagaggca1440
gaagcttatctacttcaaattctgcctcatacaatacgaggaggtgcagaagtgagcgag1500
ccagcggagcttttgttctacattcaggatagtgatgatgtctatggcctaataacattt1560
tttcctatggaaaaccagaagattgaaagcagcccaggtgaacgatacttatccttgagt1620
tttacaagactaggagggactaaaggagatgtgaggttgctttattctgtactttacatt1680
cctgctggagctgtggaccccttgcaagcaaaagaaggcatcttaaatatatcaaggaga1740
aatgacctcatttttccagagcaaaaaactcaagtcactacaaaattaccaataagaaat1800
gatgcattccttcaaaatggagctcactttctagtacagttggaaactgtggagttgtta1860
aacataattcctctaatcccacccataagccctagatttggggaaatctgcaatatttct1920
ttactggttactccagccattgcaaatggagaaattggctttctcagcaatcttccaatt1980
attttgcatgaaccagaagattttgctgctgaagtggtatacattcccttacatcgggat2040
ggaactgatggccaggctactgtctactggagtttgaagccctctggctttaattcaaaa2100
gcagtgaccccggatgatataggcccctttaatggctctgttttgtttttatctgggcaa2160
agtgacacaacaatcaacattactatcaaaggtgatgacataccggaaatgaatgaaact2220
gtaacactttctctagacagggttaacgtggaaaaccaagtgctgaaatctggatatact2280
agccgtgacctaattattttggaaaatgatgaccctgggggagtttttgaattttctcct2340
gcttccagaggaccctatgttataaaagaaggagaatctgtagagctccacatcatccga2400
tcaagggggtcccttgttaagcagtttctacactaccgagtagagccaagagatagcaat2460
gaattctatggaaacacgggagtactagaatttaaacctggagaaagggagatagtgatc2520
accttgctagcaagattggatgggataccagagttggatgaacactactgggtggtcctc2580
agcagccacggagaacgggaaagcaagttgggaagtgccaccattgtcaatataacgatt2640
ctgaaaaatgatgatcctcatggcattatagaatttgtttctgatggtctaattgtgatg2700
ataaatgaaagcaaaggagatgctatctatagtgctgtttatgatgtagtaagaaatcga2760
ggcaactttggtgatgttagtgtatcatgggtggttagtccagactttacacaagatgta2820
tttcctgtacaagggactgttgtctttggagatcaggaattttcaaaaaatatcaccatt2880
tactcccttccagatgagattccagaagaaatggaagaatttaccgttatcctactgaat2940
ggcactggaggagctaaagtgggaaatagaacaactgcaactctgaggattagaagaaat3000
gatgaccccatttattttgcagaacctcgtgtagtgagggttcaggaaggtgagactgcc3060
aactttacagttctcagaaatggatctgttgatgtgacttgcatggtccagtatgctacc3120
aaggatgggaaggctactgcaagagagagagatttcattcctgttgaaaaaggagaaacg3180
ctcatttttgaggttggaagtagacagcagagcatatccatatttgttaatgaagatggt3240
atcccggaaacagatgagcccttttatataatcctcttgaattcaacaggtgatacagta3300
gtatatcaatatggagtagctacagtaataattgaagctaatgatgacccaaatggcatt3360
ttttctctggagcccatagacaaagcagtggaagaaggaaagactaatgcattttggatt3420
ttgaggcaccgaggatactttggtagtgtttctgtatcttggcagctctttcagaatgat3480
tctgctttgcagcctgggcaggagttctatgaaacttcaggaactgttaacttcatggat3540
ggagaagaagcaaaaccaatcattctccatgcttttccagataaaattcctgaattcaat3600
gaattttatttcctaaaacttgtaaacatttcaggtggatccccaggtcctgggggccag3660
ctagcagaaaccaacctccaggtgacagtaatggttccattcaatgatgatccctttgga3720
gtttttatcttggatccagagtgtttagagagagaagtggcagaagatgtcctgtctgaa3780
gatgatatgtcttatattaccaacttcaccattttgaggcagcagggtgtgtttggtgat3840
gtacaactgggctgggaaatactgtccagtgagttccctgctggtttgccaccaatgata3900
gattttttactggttggaattttccccaccaccgtgcatttacaacagcacatgcggcgt3960
caccacagtggaacggatgctttgtactttaccggactagagggtgcatttgggactgtt4020
aatccaaaataccatccctccaggaataatacaattgccaactttacattctcagcttgg4080
gtaatgcccaatgccaatacgaatggattcattatagcgaaggatgacggtaatggaagc4140
atctactacggggtaaaaatacaaacaaacgaatcccatgtgacactttcccttcattat4200
aaaaccttgggttccaatgctacatacattgccaagacaacagtcatgaaatatttagaa4260
gaaagtgtttggcttcatctactaattatcctggaggatggtataatcgaattctacctg4320
gatggaaatgcaatgcccaggggaatcaagagtctgaaaggagaagccattactgacggt4380
cctgggatactgagaattggagcagggataaatggcaatgacagatttacaggtctgatg4440
caggatgtgaggtcctatgagcggaaactgacgcttgaagaaatttatgaacttcatgcc4500
atgcccgcaaaaagtgatttacacccaatttctggatatctggagttcagacagggagaa4560
actaacaaatcattcattatttctgcaagagatgacaatgacgaggaaggagaagaatta4620
ttcattcttaaactagtttctgtatatggaggagctcgtatttcggaagaaaatactact4680
gcaagattaacaatacaaaaaagtgacaatgcaaatggcttgtttggtttcacaggagct4740
tgtataccagagattgcagaggagggatcaaccatttcttgtgtggttgagagaaccaga4800
ggagctctggattatgtgcatgttttttacaccatttcacagattgaaactgatggcatt4860
aattaccttgttgatgactttgctaatgccagtggaactattacattccttccttggcag4920
agatcagaggttctgaatatatatgttcttgatgatgatattcctgaacttaatgagtat4980
ttccgtgtgacattggtttctgcaattcctggagatgggaagctaggctcaactcctacc5040
agtggtgcaagcatagatcctgaaaaggaaacgactgatatcaccatcaaagctagtgat5100
catccatatggcttgctgcagttctccacagggctgcctcctcagcctaaggacgcaatg5160
accctgcctgcaagcagcgttccacatatcactgtggaggaggaagatggagaaatcagg5220
ttattggtcatccgtgcacagggacttctgggaagggtgactgcggaatttagaacagtg5280
tccttgacagcattcagtcctgaggattaccagaatgttgctggcacattagaatttcaa5340
ccaggagaaagatataaatacattttcataaacatcactgataattctattcctgaactg5400
gaaaaatcttttaaagttgagttgttaaacttggaaggaggagtagctgaactctttagg5460
gttgatggaagtggtagtggtgatggggacatggaattcttccttccaactattcacaaa5520
cgtgccagtctaggagtggcttcccaaattctagtgacaattgcagcctctgaccacgct5580
catggcgtatttgaatttagccctgagtcactctttgtcagtggaactgaaccagaagat5640
gggtatagcactgttacattaaatgttataagacatcatggaactctgtctccagtgact5700
ttgcattggaacatagactctgatcctgatggtgatctcgccttcacctctggcaacatc5760
acatttgagattgggcagacgagcgccaatatcactgtggagatattgcctgacgaagac5820
ccagaactggataaggcattctctgtgtcagtcctcagtgtttccagtggttctttggga5880
gctcatattaatgccacgttaacagttttggctagtgatgatccatatgggatattcatt5940
ttttctgagaaaaacagacctgttaaagttgaggaagcaacccagaacatcacactatca6000
ataataaggttgaaaggcctcatgggaaaagtccttgtctcatatgcaacactagatgat6060
atggaaaaaccaccttattttccacctaatttagcgagagcaactcaaggaagagactat6120
ataccagcttctggatttgctctttttggagctaatcagagtgaggcaacaatagctatt6180
tcaattttggatgatgatgagccagaaaggtccgaatctgtctttatcgaactactcaac6240
tctactttagtagcgaaagtacagagtcgttcaattccaaattctccacgtcttgggcct6300
aaggtagaaactattgcgcaactaattatcattgccaatgatgatgcatttggaactctt6360
cagctctcagcaccaattgtccgagtggcagaaaatcatgttggacccattatcaatgtg6420
actagaacaggaggagcatttgcagatgtctctgtgaagtttaaagctgtgccaataact6480
gcaatagctggtgaagattatagtatagcttcatcagatgtggtcttgctagaaggggaa6540
accagtaaagccgtgccaatatatgtcattaatgatatctatcctgaactggaagaatct6600
tttcttgtgcaactgatgaatgaaacaacaggaggagccagactaggggctttaacagag6660
gcagtcattattattgaggcctctgatgacccctatggattatttggttttcagattact6720
aaacttattgtagaggaacctgagtttaactcagtgaaggtaaacctgccaataattcga6780
aattctgggacactcggcaatgttactgttcggtgggttgccaccattaatggacagctt6840
gctactggcgacctgcgagttgtctcaggtaatgtgacctttgcccctggggaaaccatt6900
caaaccttgttgttagaggtcctggctgacgacgttccggagattgaagaggttatccaa6960
gtgcaactaactgatgcctctggtggaggtactattgggttagatcgaattgcaaatatt7020
attattcctgccaatgatgatccttatggtacagtagcctttgctcagatggtttatcgt7080
gttcaagagcctctggaaagaagttcctgtgctaatataactgtcaggcgaagcggaggg7140
cactttggtcggctgttgttgttctacagtacttccgacattgatgtagtggctctggca7200
atggaggaaggtcaagatttactgtcctactatgaatctccaattcaaggggtgcctgac7260
ccactttggagaacttggatgaatgtctctgccgtgggggagcccctgtatacctgtgcc7320
actttgtgccttaaggaacaagcttgctcagcgttttcatttttcagtgcttctgagggt7380
ccccagtgtttctggatgacatcatggatcagcccagctgtcaacaattcagacttctgg7440
acctacaggaaaaacatgaccagggtagcatctctttttagtggtcaggctgtggctggg7500
agtgactatgagcctgtgacaaggcaatgggccataatgcaggaaggtgatgaattcgca7560
aatctcacagtgtctattcttcctgatgatttcccagagatggatgagagttttctaatt7620
tctctccttgaagttcacctcatgaacatttcagccagtttgaaaaatcagccaaccata7680
ggacagccaaatatttctacagttgtcatagcactaaatggtgatgcctttggagtgttt7740
gtgatctacaatattagtcccaatacttccgaagatggcttatttgttgaagttcaggag7800
cagccccaaaccttggtggagctgatgatacacaggacagggggcagcttaggtcaagtg7860
gcagtcgaatggcgtgttgttggtggaacagctactgaaggtttagattttataggtgct7920
ggagagattctgacctttgctgaaggtgaaaccaaaaagacagtcattttaaccatcttg7980
gatgactctgaaccagaggatgacgaaagtatcatagttagtttggtgtacactgaaggt8040
ggaagtagaattttgccaagctccgacactgttagagtgaacattttggccaatgacaat8100
gtggcaggaattgttagctttcagacagcttccagatctgtcataggtcatgaaggagaa8160
attttacaattccatgtgataagaactttccctggtcgaggaaatgttactgttaactgg8220
aaaattattgggcaaaatctagaactcaattttgctaactttagcggacaacttttcttt8280
cctgaggggtcgttgaatacaacattgtttgtgcatttgttggatgacaacattcctgag8340
gagaaagaagtataccaagtcattctgtatgatgtcaggacacaaggagttccaccagcc8400
ggaatcgccctgcttgatgctcaaggatatgcagctgtcctcacagtagaagccagtgat8460
gaaccacatggagttttaaattttgctctttcatcaagatttgtgttactacaagaggct8520
aacataacaattcagcttttcatcaacagagaatttggatctctaggagctatcaatgtc8580
acatataccacggttcctggaatgctgagtctgaagaaccaaacagtaggaaacctagca8640
gagccagaagttgattttgtccctatcattggctttctgattttagaagaaggggaaaca8700
gcagcagccatcaacattaccattcttgaggatgatgtaccagagctagaagaatatttc8760
ctggtgaatttaacttacgttggacttaccatggctgcttcaacttcatttcctcccaga8820
ctagattcagaaggtttgactgcacaagttattattgatgccaatgatggggcccgaggt8880
gtaattgaatggcaacaaagcaggtttgaagtaaatgaaacccatggaagtttaacattg8940
gtagcccagaggagcagagaacctcttggccatgtttccttatttgtgtatgctcagaat9000
ttggaagcacaagtggggctggattatatcttcaccccaatgattcttcattttgctgat9060
ggagaaaggtataaaaatgtcaatatcatgattcttgatgatgacattccagaaggagat9120
gaaaaatttcagctgattttaacaaatccttctcctggactagagctagggaaaaataca9180
atagccttaattattgtccttgctaatgatgacggccctggagttctatcatttaacaac9240
agtgagcactttttcctaagagagccaacagctctctacgtccaggagagtgttgcagta9300
ttgtacattgttcgggaacctgcacaaggattgtttggaacagtgacagttcagttcatt9360
gtgacagaagtgaattcctcaaatgaatctaaagatctgactccttccaaaggctatatt9420
gttttagaagaaggtgttcgattcaaggccctacaaatatctgccatattagacacggaa9480
ccagaaatggatgagtattttgtttgcaccttgtttaatccaactggaggtgctagacta9540
ggggtgcatgttcaaaccctgataacagttttgcaaaaccaggcccctttggggctattc9600
agtatctctgcagttgaaaatagagccacctccatagacatcgaagaagccaataggacc9660
gtgtatttaaatgtatctcgaactaatggcattgatttggctgtgagtgtgcagtgggag9720
acagtatctgaaacagcctttggcatgaggggaatggatgttgtgttttccgtatttcaa9780
agttttttggatgaatcagcttctggctggtgtttctttactttggaaaatttaatatat9840
ggtataatgttaagaaaatcatctgttactgtttaccgatggcaggggatttttattcca9900
gttgaggatttaaatatagaaaatcctaaaacttgtgaggcctttaatattggtttttct9960
ccctactttgtgattactcatgaagaaagaaatgaagaaaagccttctcttaacagtgtg10020
tttacattcacatctggatttaaattattcctggtacaaacaatcattattctggaaagt10080
tctcaagtaagatattttacttcagacagccaagattatttaatcattgcaagtcaaaga10140
gatgattccgaattaactcaggtcttcaggtggaatggaggaagcttcgtgttgcatcaa10200
aaactccctgtccgaggtgtgctgaccgtggccttgttcaacaagggaggctctgtgttc10260
ttagccatttcccaggctaatgccaggctaaactcccttttattcagatggtctggcagt10320
gggtttattaactttcaagaggtgcctgtcagtgggacaacagaagttgaggctttgtct10380
tcagccaatgatatttacctaatatttgccgaaaatgtctttctaggagatcagaattca10440
attgatattttcatctgggagatgggacagtcttccttcaggtattttcagtctgtagat10500
tttgctgctgttaacagaatccactccttcacaccagcctcaggaatagcccacatactt10560
cttattggccaagatatgtctgctctttactgctggaattcggagcgtaatcaattctct10620
tttgttctggaagtaccttctgcttatgatgtggcttctgttacagtaaagtcccttaat10680
tcaagcaagaatttaatagctctagtgggagctcattcacatatatatgagctagcctac10740
atttccagccattctgactttattcctagttcaggtgaactgatatttgaacctggtgag10800
agagaagctacaatagcagtaaatatccttgatgatacagttccagaaaaagaagaatcc10860
ttcaaagttcaacttaaaaatcccaaaggaggagcagagattggcattaatgattctgta10920
acaataaccattctgtctaatgatgatgcctatggaattgttgcatttgctcagaattca10980
ttatataagcaagtggaagaaatggagcaagatagcctagtaaccttgaacgttgaacgc11040
ttaaaaggaacatatggccgtataaccatagcatgggaagctgatggaagtattagtgat11100
atatttcctacctcaggagtgattttatttactgaaggccaggtactgtcaacaatcact11160
ctaactattcttgctgataatataccagagttatcagaggttgtgattgtaaccctcacc11220
cgtatcaccacagaaggggttgaggactcatacaaaggtgctactattgatcaggacaga11280
agcaagtctgttataacaactttgcccaatgactcaccttttggcttggtgggctggcgt11340
gctgcgtctgtcttcattagagtagcagagcctaaagaaaacaccaccactcttcagtta11400
caaatagctcgagataaaggactacttggggatattgccattcacttgagagctcaaccc11460
aatttcttactgcatgtcgataatcaagctactgagaatgaagattatgtattgcaagaa11520
acaataataataatgaaagaaaacataaaagaagctcatgccgaagtttccattttgccg11580
gatgaccttcctgaattggaggaaggatttattgtcactatcactgaggtgaacctggtg11640
aactctgacttctctacaggacagccaagtgtgcggaggcccggaatggaaatagctgag11700
ataatgatagaagaaaatgacgatcccagaggaatttttatgtttcatgttactagaggc11760
gctggggaagttattactgcctatgaggtgcctccacccttgaacgttcttcaagttcct11820
gtagtccggctggctggaagctttggggcagtaaatgtttattggaaagcatcaccagac11880
agtgctggcctggaagactttaaaccatctcatgggattcttgaatttgcagataaacag11940
gttactgcaatgatagaaatcaccataattgatgatgctgaatttgaattgacagagacg12000
ttcaatatttccttgatcagtgttgctggaggtggcagacttggtgatgatgttgtggta12060
actgttgttattccacaaaatgattctccatttggagtatttggatttgaagaaaagact12120
gtaatgattgatgaatccctttcatccgatgaccctgattcatatgtgacattgacggtt12180
gtccggtccccaggaggaaaaggaaccgtccgacttgagtggaccatagatgagaaggct12240
aaacataaccttagtcctttgaatgggacccttcattttgatgagactgagtcccagaag12300
accattgtgttgcacacacttcaagacacagtgttggaggaggacaggcgtttcaccatt12360
cagctgatatcaattgatgaggtagaaatatctccagtaaaaggtagtgcatcaataatt12420
attcggggtgataagcgagcatcaggagaagttgggatagctccgtcatctaggcacatc12480
ctcattggggaaccctcagcaaaatataatggtaccgctattatcagccttgttcgaggc12540
ccagggattttgggggaggtcacagtgttctggaggatattccctccttccgtgggggaa12600
tttgctgaaacatcaggaaaactgacaatgcgagacgaacagtctgcagtcattgtagta12660
atacaggctttgaacgatgacattcccgaggaaaaaagcttctatgagtttcagctcact12720
gcagtcagtgagggaggagttctgagtgaatccagcagcactgccaacatcacggtggtg12780
gccagcgactctccctatggccgatttgccttttcacatgagcaacttcgagtgtcagaa12840
gcacagagggttaacatcacaatcatccgttccagtggagattttggccatgtgcgactc12900
tggtacaagacgatgagcgggacagcggaagcaggcttggattttgttcctgcagcaggg12960
gagctcctctttgaagcaggggagatgaggaaaagtctgcatgttgaaatccttgatgat13020
gactatcctgaaggcccagaggaattttctctaacaattacaaaggtggaactccaggga13080
agagggtatgattttaccattcaagaaaatggacttcagatagatcaacctcctgaaata13140
ggaaacatctccattgttcgcatcataataatgaaaaatgataacgcagaaggcatcatt13200
gaatttgacccaaagtatactgccttcgaagtggaggaagatgttgggctgatcatgatc13260
ccagtggtgaggctacatggaacttatggctatgtgacagctgatttcatctctcagagc13320
tcctctgccagtcccggaggtgttgattacattttgcatggcagtacagtcacctttcag13380
catgggcaaaacttaagttttataaatatctccatcattgatgacaatgaaagtgaattt13440
gaggagcccattgaaattctactcactggagctactggaggagcggtccttgggcgccac13500
ctagtgagcagaatcataatagctaagagtgactctccctttggagttataaggtttctc13560
aatcaaagcaaaatttctattgctaatcccaattccacaatgattttatcactggtgctg13620
gagcggactggaggactcttgggagagattcaggtgaactgggagacagtaggacccaac13680
tctcaagaagccttactgccacagaatagagacattgcagacccagtgagcgggttgttc13740
tattttggagaaggagaaggaggagtgagaaccataattctgacaatctatcctcatgaa13800
gaaattgaagttgaagagacattcattattaaacttcatcttgtgaaaggagaagctaaa13860
ttagactccagagctaaagatgttacattaaccatacaagagtttggtgacccaaatgga13920
gttgttcagtttgctcctgaaactttgtctaagaagacttattcagagcctctggctctg13980
gaagggcccctgctcattaccttctttgtcagaagagtcaagggcacctttggagagatt14040
atggtttactgggaattaagtagtgagtttgacattactgaagactttctttccaccagt14100
ggatttttcaccattgctgatggagagagtgaagctagctttgatgttcatttgctacca14160
gatgaggtacctgagatagaggaagattatgtgatccagcttgtttctgtagagggagga14220
gccgaactggatctggagaagagtatcacatggttctctgtttatgcaaatgatgaccca14280
catggagtatttgccctgtattcggatcgccagtcaatacttattgggcagaaccttatt14340
agatccatccaaattaacataacccggcttgctggaacatttggagatgtggctgttggg14400
cttcgaatatcatcggatcataaagaacagccgattgttaccgaaaatgcagagaggcag14460
ctggtggtcaaagatggtgccacatataaagtggacgtggtgccaataaagaatcaggtc14520
ttcctatcactgggctctaatttcactttgcaactggtgactgtgatgcttgtcggtgga14580
cgtttctatggaatgccaacaattcttcaggaagcaaaatctgctgtccttccagtctct14640
gagaaagctgccaattctcaggtcggatttgaatccactgcttttcaactcatgaacatc14700
actgctggcacaagccacgttatgatttctaggagaggcacatatggagctctctcggtt14760
gcctggaccactggatatgctcctgggttagaaattcctgaattcattgttgttggcaac14820
atgaccccaacactggggagcctttcattttcccacggtgaacaaaggaaaggagttttc14880
ctgtggacgtttcctagccctggttggccagaggcctttgttcttcacctatcaggagtg14940
cagagcagtgctcctggcggagctcaactccgatcaggtttcattgttgctgaaattgaa15000
ccaatgggcgtcttccaattttccactagctcaagaaatatcatagtgtcagaagataca15060
cagatgatcagattacatgtacaaagactatttgggttccacagcgatcttattaaagtt15120
tcttatcagaccactgcaggaagcgccaagccactggaagattttgagcctgttcagaat15180
ggggaactgttttttcaaaaattccaaactgaggttgattttgaaataaccattattaat15240
gatcagctttctgagatagaagaatttttttacattaaccttacttcagtagaaattagg15300
ggattacaaaagtttgatgttaattggagcccacgcctgaatctagatttcagtgttgca15360
gtgattacaatattggataatgatgacctggcaggaatggatatttccttccccgagaca15420
actgtggctgtagcagttgacacaactctcattcctgtagaaactgaatccaccacatac15480
ctcagcacaagcaagacgactaccattctgcagccaaccaacgtggttgccattgttact15540
gaggcaactggtgtatctgccatccctgagaaacttgtcacccttcatggcacacctgct15600
gtgtctgaaaagcctgatgtggccactgtaactgccaatgtttccattcatggaacattc15660
agccttgggccatccattgtttatattgaagaggagatgaagaatggcacattcaacact15720
gcagaagttcttatccgaagaactggtgggtttactggcaatgtcagcataacagttaaa15780
actttcggtgaaagatgtgctcagatggaaccaaatgcattgccctttcgtggtatctat15840
gggatttccaacctaacatgggcagttgaagaagaagactttgaagaacaaactcttacc15900
cttatattcctagatggagaaagagaacgtaaagtatcagttcaaattttggatgatgat15960
gagcctgaggggcaggaattcttctacgtgtttctcacaaaccctcaagggggagcacag16020
attgtggaggagaaggatgatactggatttgcagcttttgccatggttattattacaggg16080
agtgaccttcacaatggcatcataggattcagtgaggagtcccagagtggactagaactc16140
agggaaggagctgttatgagaagattgcaccttattgtcacaagacagccaaacagggcc16200
tttgaagatgtcaaggtcttttggcgagtcacacttaacaaaacagtcgtcgtgctccag16260
aaggatggggtaaacctggtggaggaacttcagtctgtgtcagggaccacaacctgtaca16320
atgggtcaaacaaaatgctttatcagcattgaactcaaaccagaaaaggtaccacaggtt16380
gaagtgtatttttttgtggaactatatgaagctactgctggagcagcaataaacaacagt16440
gccagattcgcacagattaaaatcttagaaagtgatgaatctcaaagccttgtgtatttt16500
tctgtgggttctcggctggcagtggctcacaagaaggccactttaatcagtctgcaggtg16560
gccagagattctgggacaggactaatgatgtctgttaactttagtacccaggagttgagg16620
agtgctgaaacaattggtcgtaccatcatatctccagctatttctggaaaggattttgtg16680
ataactgaaggcacattggtctttgaacctggccagagaagcactgtattggatgtcatc16740
ctaacgccagagacaggatctttaaattcatttcctaaacgcttccagattgtccttttt16800
gacccaaaaggtggtgccagaattgataaagtgtatgggactgccaacatcactcttgtc16860
tcagatgcagattcgcaggccatttgggggcttgcagatcagctacatcagcctgtgaat16920
gatgatattctcaacagagtgctccataccatcagcatgaaagtggccacagaaaacaca16980
gatgaacaactcagtgccatgatgcatttaatagaaaagataactactgaaggaaaaatt17040
caagctttcagtgttgccagccgaactcttttctatgagattctttgttctcttattaac17100
ccaaagcgcaaggacactaggggattcagtcactttgctgaagtgactgagaattttgcc17160
ttttctctgctgactaatgttacttgcggctctcctggtgaaaaaagcaaaaccatcctt17220
gatagttgcccatatttgtcaatattggctcttcactggtatcctcagcaaatcaatgga17280
cacaagtttgaaggaaaggaaggagattacattcgaattccagagaggctactggatgtc17340
caggatgcagaaataatggctgggaaaagtacatgtaaattagtccagtttacagagtat17400
agcagccaacagtggtttataagtggaaacaatcttcctaccctaaaaaataaggtatta17460
tctttgagtgtgaaaggtcagagttcacaactcctgactaatgacaatgaggttctctac17520
aggatttatgctgctgagcctagaattattcctcagacatctctgtgtctcctttggaat17580
caggctgctgcaagctggttgtctgacagtcagttttgcaaagtggttgaggaaactgca17640
gactatgtggaatgtgcctgttcacacatgtctgtgtatgctgtctatgctcggactgac17700
aacttgtcttcatacaatgaagccttcttcacttctggatttatatgtatctcaggtctt17760
tgcttggctgttctttcccatatcttctgtgccaggtactccatgtttgcagctaaactt17820
ctgactcacatgatggcagccagcttaggtacacagattctgtttctggcgtctgcatac17880
gcaagtccccaactcgctgaggagagctgttcagctatggctgctgtcacacattacctg17940
tatctttgccagtttagctggatgctcattcagtctgtgaatttctggtacgtgctggtg18000
atgaatgatgagcacacagagaggcgatatctgctgtttttccttctgagttggggacta18060
ccagcttttgtggtgattctcctcatagttattttgaaaggaatctatcatcagagcatg18120
tcacagatctatggactcattcatggtgacctgtgttttattccaaacgtctatgctgct18180
ttgttcactgcagctcttgttcctttgacgtgcctcgtggtggtgttcgtggtgttcatc18240
catgcctaccaggtgaagccacagtggaaagcatatgatgatgtcttcagaggaaggaca18300
aatgctgcagaaattccactgattttatatctctttgctctgatttccgtgacatggctt18360
tggggaggactacacatggcctacagacacttctggatgttggttctctttgtcattttc18420
aacagtctgcagggactttatgttttcatggtttatttcattttacacaaccaaatgtgt18480
tgccctatgaaggccagttacactgtggaaatgaatgggcatcctggacccagcacagcc18540
tttttcacgcccgggagtggaatgcctcctgctggaggggaaatcagcaagtccacccag18600
aatctcatcggtgctatggaggaggtgccacctgactgggagagagcatccttccaacag18660
ggcagtcaggccagccctgatttaaagccaagtccacaaaatggagccacgttcccgtcc18720
tctggaggatatggccaggggtcactgatagccgatgaggagtcccaggagtttgatgat18780
ttaatatttgcattaaaaactggtgctggtctcagtgtcagtgataatgaatctggtcaa18840
ggcagccaggaggggggcaccttgactgactcccagatcgtggagctcaggaggataccc18900
atcgccgacactcacctgtag18921
<210>2
<211>6306
<212>prt
<213>人工序列
<400>2
metservalpheleuglyproglymetproseralaserleuleuval
151015
asnleuleuseralaleuleuileleuphevalpheglygluthrglu
202530
ileargphethrglyglnthrgluphevalvalasngluthrserthr
354045
thrvalileargleuileilegluargileglygluproalaasnval
505560
thralailevalserleutyrglygluaspalaglyaspphepheasp
65707580
thrtyralaalaalapheileproalaglygluthrasnargthrval
859095
tyrilealavalcysaspaspaspleuprogluproaspgluthrphe
100105110
ilephehisleuthrleuglnlysproseralaasnvallysleugly
115120125
trpproargthrvalthrvalthrileleuserasnaspasnalaphe
130135140
glyileileserpheasnmetleuproserilealavalserglupro
145150155160
lysglyargasnglusermetproleuthrleuileargglulysgly
165170175
thrtyrglymetvalmetvalthrphegluvalgluglyglyproasn
180185190
proproaspgluaspleuserprovallysglyasnilethrphepro
195200205
proglyargalathrvaliletyrasnleuthrvalleuaspaspglu
210215220
valprogluasnaspgluilepheleuileglnleulysservalglu
225230235240
glyglyalagluileasnthrserargasnserilegluileileile
245250255
lyslysasnaspserprovalargpheleuglnseriletyrleuval
260265270
proglugluasphisileleuileileprovalvalargglylysasp
275280285
asnasnglyasnleuileglyseraspglutyrgluvalserileser
290295300
tyralavalthrthrglyasnserthralahisalaglnglnasnleu
305310315320
asppheileaspleuglnproasnthrthrvalvalpheproprophe
325330335
ilehisgluserhisleulyspheglnilevalaspaspthrilepro
340345350
gluilealagluserphehisilemetleuleulysaspthrleugln
355360365
glyaspalavalleuileserproservalvalglnvalthrilelys
370375380
proasnasplysprotyrglyvalleuserpheasnservalleuphe
385390395400
gluargthrvalileileaspgluaspargileserargtyrgluglu
405410415
ilethrvalvalargasnglyglythrhisglyasnvalseralaasn
420425430
trpvalleuthrargasnserthraspproserprovalthralaasp
435440445
ileargproserserglyvalleuhisphealaglnglyglnmetleu
450455460
alathrileproleuthrvalvalaspaspaspleuproglugluala
465470475480
glualatyrleuleuglnileleuprohisthrileargglyglyala
485490495
gluvalsergluproalagluleuleuphetyrileglnaspserasp
500505510
aspvaltyrglyleuilethrphepheprometgluasnglnlysile
515520525
gluserserproglygluargtyrleuserleuserphethrargleu
530535540
glyglythrlysglyaspvalargleuleutyrservalleutyrile
545550555560
proalaglyalavalaspproleuglnalalysgluglyileleuasn
565570575
ileserargargasnaspleuilepheprogluglnlysthrglnval
580585590
thrthrlysleuproileargasnaspalapheleuglnasnglyala
595600605
hispheleuvalglnleugluthrvalgluleuleuasnileilepro
610615620
leuileproproileserproargpheglygluilecysasnileser
625630635640
leuleuvalthrproalailealaasnglygluileglypheleuser
645650655
asnleuproileileleuhisgluprogluaspphealaalagluval
660665670
valtyrileproleuhisargaspglythraspglyglnalathrval
675680685
tyrtrpserleulysproserglypheasnserlysalavalthrpro
690695700
aspaspileglypropheasnglyservalleupheleuserglygln
705710715720
seraspthrthrileasnilethrilelysglyaspaspileproglu
725730735
metasngluthrvalthrleuserleuaspargvalasnvalgluasn
740745750
glnvalleulysserglytyrthrserargaspleuileileleuglu
755760765
asnaspaspproglyglyvalpheglupheserproalaserarggly
770775780
protyrvalilelysgluglygluservalgluleuhisileilearg
785790795800
serargglyserleuvallysglnpheleuhistyrargvalglupro
805810815
argaspserasngluphetyrglyasnthrglyvalleugluphelys
820825830
proglygluarggluilevalilethrleuleualaargleuaspgly
835840845
ileprogluleuaspgluhistyrtrpvalvalleuserserhisgly
850855860
gluarggluserlysleuglyseralathrilevalasnilethrile
865870875880
leulysasnaspaspprohisglyileilegluphevalseraspgly
885890895
leuilevalmetileasngluserlysglyaspalailetyrserala
900905910
valtyraspvalvalargasnargglyasnpheglyaspvalserval
915920925
sertrpvalvalserproaspphethrglnaspvalpheprovalgln
930935940
glythrvalvalpheglyaspglnglupheserlysasnilethrile
945950955960
tyrserleuproaspgluileprogluglumetglugluphethrval
965970975
ileleuleuasnglythrglyglyalalysvalglyasnargthrthr
980985990
alathrleuargileargargasnaspaspproiletyrphealaglu
99510001005
proargvalvalargvalglngluglygluthralaasnphethr
101010151020
valleuargasnglyservalaspvalthrcysmetvalglntyr
102510301035
alathrlysaspglylysalathralaarggluargasppheile
104010451050
provalglulysglygluthrleuilephegluvalglyserarg
105510601065
glnglnserileserilephevalasngluaspglyileproglu
107010751080
thraspgluprophetyrileileleuleuasnserthrglyasp
108510901095
thrvalvaltyrglntyrglyvalalathrvalileilegluala
110011051110
asnaspaspproasnglyilepheserleugluproileasplys
111511201125
alavalglugluglylysthrasnalaphetrpileleuarghis
113011351140
argglytyrpheglyservalservalsertrpglnleuphegln
114511501155
asnaspseralaleuglnproglyglngluphetyrgluthrser
116011651170
glythrvalasnphemetaspglygluglualalysproileile
117511801185
leuhisalapheproasplysileproglupheasngluphetyr
119011951200
pheleulysleuvalasnileserglyglyserproglyprogly
120512101215
glyglnleualagluthrasnleuglnvalthrvalmetvalpro
122012251230
pheasnaspasppropheglyvalpheileleuaspproglucys
123512401245
leugluarggluvalalagluaspvalleusergluaspaspmet
125012551260
sertyrilethrasnphethrileleuargglnglnglyvalphe
126512701275
glyaspvalglnleuglytrpgluileleusersergluphepro
128012851290
alaglyleuproprometileasppheleuleuvalglyilephe
129513001305
prothrthrvalhisleuglnglnhismetargarghishisser
131013151320
glythraspalaleutyrphethrglyleugluglyalaphegly
132513301335
thrvalasnprolystyrhisproserargasnasnthrileala
134013451350
asnphethrpheseralatrpvalmetproasnalaasnthrasn
135513601365
glypheileilealalysaspaspglyasnglyseriletyrtyr
137013751380
glyvallysileglnthrasngluserhisvalthrleuserleu
138513901395
histyrlysthrleuglyserasnalathrtyrilealalysthr
140014051410
thrvalmetlystyrleuglugluservaltrpleuhisleuleu
141514201425
ileileleugluaspglyileilegluphetyrleuaspglyasn
143014351440
alametproargglyilelysserleulysglyglualailethr
144514501455
aspglyproglyileleuargileglyalaglyileasnglyasn
146014651470
aspargphethrglyleumetglnaspvalargsertyrgluarg
147514801485
lysleuthrleuglugluiletyrgluleuhisalametproala
149014951500
lysseraspleuhisproileserglytyrleugluphearggln
150515101515
glygluthrasnlysserpheileileseralaargaspaspasn
152015251530
aspglugluglyglugluleupheileleulysleuvalserval
153515401545
tyrglyglyalaargileserglugluasnthrthralaargleu
155015551560
thrileglnlysseraspasnalaasnglyleupheglyphethr
156515701575
glyalacysileprogluilealaglugluglyserthrileser
158015851590
cysvalvalgluargthrargglyalaleuasptyrvalhisval
159516001605
phetyrthrileserglnilegluthraspglyileasntyrleu
161016151620
valaspaspphealaasnalaserglythrilethrpheleupro
162516301635
trpglnargsergluvalleuasniletyrvalleuaspaspasp
164016451650
ileprogluleuasnglutyrpheargvalthrleuvalserala
165516601665
ileproglyaspglylysleuglyserthrprothrserglyala
167016751680
serileaspproglulysgluthrthraspilethrilelysala
168516901695
serasphisprotyrglyleuleuglnpheserthrglyleupro
170017051710
proglnprolysaspalametthrleuproalaserservalpro
171517201725
hisilethrvalgluglugluaspglygluileargleuleuval
173017351740
ileargalaglnglyleuleuglyargvalthralagluphearg
174517501755
thrvalserleuthralapheserprogluasptyrglnasnval
176017651770
alaglythrleuglupheglnproglygluargtyrlystyrile
177517801785
pheileasnilethraspasnserileprogluleuglulysser
179017951800
phelysvalgluleuleuasnleugluglyglyvalalagluleu
180518101815
pheargvalaspglyserglyserglyaspglyaspmetgluphe
182018251830
pheleuprothrilehislysargalaserleuglyvalalaser
183518401845
glnileleuvalthrilealaalaserasphisalahisglyval
185018551860
pheglupheserprogluserleuphevalserglythrglupro
186518701875
gluaspglytyrserthrvalthrleuasnvalilearghishis
188018851890
glythrleuserprovalthrleuhistrpasnileaspserasp
189519001905
proaspglyaspleualaphethrserglyasnilethrpheglu
191019151920
ileglyglnthrseralaasnilethrvalgluileleuproasp
192519301935
gluaspprogluleuasplysalapheservalservalleuser
194019451950
valserserglyserleuglyalahisileasnalathrleuthr
195519601965
valleualaseraspaspprotyrglyilepheilepheserglu
197019751980
lysasnargprovallysvalgluglualathrglnasnilethr
198519901995
leuserileileargleulysglyleumetglylysvalleuval
200020052010
sertyralathrleuaspaspmetglulysproprotyrphepro
201520202025
proasnleualaargalathrglnglyargasptyrileproala
203020352040
serglyphealaleupheglyalaasnglnserglualathrile
204520502055
alaileserileleuaspaspaspgluprogluargsergluser
206020652070
valpheilegluleuleuasnserthrleuvalalalysvalgln
207520802085
serargserileproasnserproargleuglyprolysvalglu
209020952100
thrilealaglnleuileileilealaasnaspaspalaphegly
210521102115
thrleuglnleuseralaproilevalargvalalagluasnhis
212021252130
valglyproileileasnvalthrargthrglyglyalapheala
213521402145
aspvalservallysphelysalavalproilethralaileala
215021552160
glygluasptyrserilealaserseraspvalvalleuleuglu
216521702175
glygluthrserlysalavalproiletyrvalileasnaspile
218021852190
tyrprogluleuglugluserpheleuvalglnleumetasnglu
219522002205
thrthrglyglyalaargleuglyalaleuthrglualavalile
221022152220
ileileglualaseraspaspprotyrglyleupheglyphegln
222522302235
ilethrlysleuilevalglugluproglupheasnservallys
224022452250
valasnleuproileileargasnserglythrleuglyasnval
225522602265
thrvalargtrpvalalathrileasnglyglnleualathrgly
227022752280
aspleuargvalvalserglyasnvalthrphealaproglyglu
228522902295
thrileglnthrleuleuleugluvalleualaaspaspvalpro
230023052310
gluileglugluvalileglnvalglnleuthraspalasergly
231523202325
glyglythrileglyleuaspargilealaasnileileilepro
233023352340
alaasnaspaspprotyrglythrvalalaphealaglnmetval
234523502355
tyrargvalglngluproleugluargsersercysalaasnile
236023652370
thrvalargargserglyglyhispheglyargleuleuleuphe
237523802385
tyrserthrseraspileaspvalvalalaleualametgluglu
239023952400
glyglnaspleuleusertyrtyrgluserproileglnglyval
240524102415
proaspproleutrpargthrtrpmetasnvalseralavalgly
242024252430
gluproleutyrthrcysalathrleucysleulysgluglnala
243524402445
cysseralapheserphepheseralasergluglyproglncys
245024552460
phetrpmetthrsertrpileserproalavalasnasnserasp
246524702475
phetrpthrtyrarglysasnmetthrargvalalaserleuphe
248024852490
serglyglnalavalalaglyserasptyrgluprovalthrarg
249525002505
glntrpalailemetglngluglyaspgluphealaasnleuthr
251025152520
valserileleuproaspasppheproglumetaspgluserphe
252525302535
leuileserleuleugluvalhisleumetasnileseralaser
254025452550
leulysasnglnprothrileglyglnproasnileserthrval
255525602565
valilealaleuasnglyaspalapheglyvalphevaliletyr
257025752580
asnileserproasnthrsergluaspglyleuphevalgluval
258525902595
glngluglnproglnthrleuvalgluleumetilehisargthr
260026052610
glyglyserleuglyglnvalalavalglutrpargvalvalgly
261526202625
glythralathrgluglyleuasppheileglyalaglygluile
263026352640
leuthrphealagluglygluthrlyslysthrvalileleuthr
264526502655
ileleuaspaspsergluprogluaspaspgluserileileval
266026652670
serleuvaltyrthrgluglyglyserargileleuproserser
267526802685
aspthrvalargvalasnileleualaasnaspasnvalalagly
269026952700
ilevalserpheglnthralaserargservalileglyhisglu
270527102715
glygluileleuglnphehisvalileargthrpheproglyarg
272027252730
glyasnvalthrvalasntrplysileileglyglnasnleuglu
273527402745
leuasnphealaasnpheserglyglnleuphepheproglugly
275027552760
serleuasnthrthrleuphevalhisleuleuaspaspasnile
276527702775
progluglulysgluvaltyrglnvalileleutyraspvalarg
278027852790
thrglnglyvalproproalaglyilealaleuleuaspalagln
279528002805
glytyralaalavalleuthrvalglualaseraspgluprohis
281028152820
glyvalleuasnphealaleuserserargphevalleuleugln
282528302835
glualaasnilethrileglnleupheileasnarggluphegly
284028452850
serleuglyalaileasnvalthrtyrthrthrvalproglymet
285528602865
leuserleulysasnglnthrvalglyasnleualagluproglu
287028752880
valaspphevalproileileglypheleuileleugluglugly
288528902895
gluthralaalaalaileasnilethrileleugluaspaspval
290029052910
progluleugluglutyrpheleuvalasnleuthrtyrvalgly
291529202925
leuthrmetalaalaserthrserpheproproargleuaspser
293029352940
gluglyleuthralaglnvalileileaspalaasnaspglyala
294529502955
argglyvalileglutrpglnglnserargphegluvalasnglu
296029652970
thrhisglyserleuthrleuvalalaglnargserargglupro
297529802985
leuglyhisvalserleuphevaltyralaglnasnleugluala
299029953000
glnvalglyleuasptyrilephethrprometileleuhisphe
300530103015
alaaspglygluargtyrlysasnvalasnilemetileleuasp
302030253030
aspaspileprogluglyaspglulyspheglnleuileleuthr
303530403045
asnproserproglyleugluleuglylysasnthrilealaleu
305030553060
ileilevalleualaasnaspaspglyproglyvalleuserphe
306530703075
asnasnsergluhisphepheleuarggluprothralaleutyr
308030853090
valglngluservalalavalleutyrilevalarggluproala
309531003105
glnglyleupheglythrvalthrvalglnpheilevalthrglu
311031153120
valasnserserasngluserlysaspleuthrproserlysgly
312531303135
tyrilevalleuglugluglyvalargphelysalaleuglnile
314031453150
seralaileleuaspthrgluproglumetaspglutyrpheval
315531603165
cysthrleupheasnprothrglyglyalaargleuglyvalhis
317031753180
valglnthrleuilethrvalleuglnasnglnalaproleugly
318531903195
leupheserileseralavalgluasnargalathrserileasp
320032053210
ilegluglualaasnargthrvaltyrleuasnvalserargthr
321532203225
asnglyileaspleualavalservalglntrpgluthrvalser
323032353240
gluthralapheglymetargglymetaspvalvalpheserval
324532503255
pheglnserpheleuaspgluseralaserglytrpcysphephe
326032653270
thrleugluasnleuiletyrglyilemetleuarglysserser
327532803285
valthrvaltyrargtrpglnglyilepheileprovalgluasp
329032953300
leuasnilegluasnprolysthrcysglualapheasnilegly
330533103315
pheserprotyrphevalilethrhisglugluargasngluglu
332033253330
lysproserleuasnservalphethrphethrserglyphelys
333533403345
leupheleuvalglnthrileileileleugluserserglnval
335033553360
argtyrphethrseraspserglnasptyrleuileilealaser
336533703375
glnargaspaspsergluleuthrglnvalpheargtrpasngly
338033853390
glyserphevalleuhisglnlysleuprovalargglyvalleu
339534003405
thrvalalaleupheasnlysglyglyservalpheleualaile
341034153420
serglnalaasnalaargleuasnserleuleupheargtrpser
342534303435
glyserglypheileasnpheglngluvalprovalserglythr
344034453450
thrgluvalglualaleuserseralaasnaspiletyrleuile
345534603465
phealagluasnvalpheleuglyaspglnasnserileaspile
347034753480
pheiletrpglumetglyglnserserpheargtyrpheglnser
348534903495
valaspphealaalavalasnargilehisserphethrproala
350035053510
serglyilealahisileleuleuileglyglnaspmetserala
351535203525
leutyrcystrpasnsergluargasnglnpheserphevalleu
353035353540
gluvalproseralatyraspvalalaservalthrvallysser
354535503555
leuasnserserlysasnleuilealaleuvalglyalahisser
356035653570
hisiletyrgluleualatyrileserserhisserasppheile
357535803585
proserserglygluleuilephegluproglygluarggluala
359035953600
thrilealavalasnileleuaspaspthrvalproglulysglu
360536103615
gluserphelysvalglnleulysasnprolysglyglyalaglu
362036253630
ileglyileasnaspservalthrilethrileleuserasnasp
363536403645
aspalatyrglyilevalalaphealaglnasnserleutyrlys
365036553660
glnvalgluglumetgluglnaspserleuvalthrleuasnval
366536703675
gluargleulysglythrtyrglyargilethrilealatrpglu
368036853690
alaaspglyserileseraspilepheprothrserglyvalile
369537003705
leuphethrgluglyglnvalleuserthrilethrleuthrile
371037153720
leualaaspasnileprogluleusergluvalvalilevalthr
372537303735
leuthrargilethrthrgluglyvalgluaspsertyrlysgly
374037453750
alathrileaspglnaspargserlysservalilethrthrleu
375537603765
proasnaspserpropheglyleuvalglytrpargalaalaser
377037753780
valpheileargvalalagluprolysgluasnthrthrthrleu
378537903795
glnleuglnilealaargasplysglyleuleuglyaspileala
380038053810
ilehisleuargalaglnproasnpheleuleuhisvalaspasn
381538203825
glnalathrgluasngluasptyrvalleuglngluthrileile
383038353840
ilemetlysgluasnilelysglualahisalagluvalserile
384538503855
leuproaspaspleuprogluleuglugluglypheilevalthr
386038653870
ilethrgluvalasnleuvalasnserasppheserthrglygln
387538803885
proservalargargproglymetgluilealagluilemetile
389038953900
glugluasnaspaspproargglyilephemetphehisvalthr
390539103915
argglyalaglygluvalilethralatyrgluvalpropropro
392039253930
leuasnvalleuglnvalprovalvalargleualaglyserphe
393539403945
glyalavalasnvaltyrtrplysalaserproaspseralagly
395039553960
leugluaspphelysproserhisglyileleugluphealaasp
396539703975
lysglnvalthralametilegluilethrileileaspaspala
398039853990
gluphegluleuthrgluthrpheasnileserleuileserval
399540004005
alaglyglyglyargleuglyaspaspvalvalvalthrvalval
401040154020
ileproglnasnaspserpropheglyvalpheglyphegluglu
402540304035
lysthrvalmetileaspgluserleuserseraspaspproasp
404040454050
sertyrvalthrleuthrvalvalargserproglyglylysgly
405540604065
thrvalargleuglutrpthrileaspglulysalalyshisasn
407040754080
leuserproleuasnglythrleuhispheaspgluthrgluser
408540904095
glnlysthrilevalleuhisthrleuglnaspthrvalleuglu
410041054110
gluaspargargphethrileglnleuileserileaspgluval
411541204125
gluileserprovallysglyseralaserileileilearggly
413041354140
asplysargalaserglygluvalglyilealaproserserarg
414541504155
hisileleuileglygluproseralalystyrasnglythrala
416041654170
ileileserleuvalargglyproglyileleuglygluvalthr
417541804185
valphetrpargilepheproproservalglygluphealaglu
419041954200
thrserglylysleuthrmetargaspgluglnseralavalile
420542104215
valvalileglnalaleuasnaspaspileprogluglulysser
422042254230
phetyrglupheglnleuthralavalsergluglyglyvalleu
423542404245
sergluserserserthralaasnilethrvalvalalaserasp
425042554260
serprotyrglyargphealapheserhisgluglnleuargval
426542704275
serglualaglnargvalasnilethrileileargsersergly
428042854290
asppheglyhisvalargleutrptyrlysthrmetserglythr
429543004305
alaglualaglyleuaspphevalproalaalaglygluleuleu
431043154320
pheglualaglyglumetarglysserleuhisvalgluileleu
432543304335
aspaspasptyrprogluglyprogluglupheserleuthrile
434043454350
thrlysvalgluleuglnglyargglytyraspphethrilegln
435543604365
gluasnglyleuglnileaspglnproprogluileglyasnile
437043754380
serilevalargileileilemetlysasnaspasnalaglugly
438543904395
ileileglupheaspprolystyrthralaphegluvalgluglu
440044054410
aspvalglyleuilemetileprovalvalargleuhisglythr
441544204425
tyrglytyrvalthralaasppheileserglnserserserala
443044354440
serproglyglyvalasptyrileleuhisglyserthrvalthr
444544504455
pheglnhisglyglnasnleuserpheileasnileserileile
446044654470
aspaspasngluserglupheglugluproilegluileleuleu
447544804485
thrglyalathrglyglyalavalleuglyarghisleuvalser
449044954500
argileileilealalysseraspserpropheglyvalilearg
450545104515
pheleuasnglnserlysileserilealaasnproasnserthr
452045254530
metileleuserleuvalleugluargthrglyglyleuleugly
453545404545
gluileglnvalasntrpgluthrvalglyproasnserglnglu
455045554560
alaleuleuproglnasnargaspilealaaspprovalsergly
456545704575
leuphetyrpheglygluglygluglyglyvalargthrileile
458045854590
leuthriletyrprohisglugluilegluvalglugluthrphe
459546004605
ileilelysleuhisleuvallysglyglualalysleuaspser
461046154620
argalalysaspvalthrleuthrileglnglupheglyasppro
462546304635
asnglyvalvalglnphealaprogluthrleuserlyslysthr
464046454650
tyrsergluproleualaleugluglyproleuleuilethrphe
465546604665
phevalargargvallysglythrpheglygluilemetvaltyr
467046754680
trpgluleuserserglupheaspilethrgluasppheleuser
468546904695
thrserglyphephethrilealaaspglygluserglualaser
470047054710
pheaspvalhisleuleuproaspgluvalprogluilegluglu
471547204725
asptyrvalileglnleuvalservalgluglyglyalagluleu
473047354740
aspleuglulysserilethrtrppheservaltyralaasnasp
474547504755
aspprohisglyvalphealaleutyrseraspargglnserile
476047654770
leuileglyglnasnleuileargserileglnileasnilethr
477547804785
argleualaglythrpheglyaspvalalavalglyleuargile
479047954800
serserasphislysgluglnproilevalthrgluasnalaglu
480548104815
argglnleuvalvallysaspglyalathrtyrlysvalaspval
482048254830
valproilelysasnglnvalpheleuserleuglyserasnphe
483548404845
thrleuglnleuvalthrvalmetleuvalglyglyargphetyr
485048554860
glymetprothrileleuglnglualalysseralavalleupro
486548704875
valserglulysalaalaasnserglnvalglyphegluserthr
488048854890
alapheglnleumetasnilethralaglythrserhisvalmet
489549004905
ileserargargglythrtyrglyalaleuservalalatrpthr
491049154920
thrglytyralaproglyleugluileproglupheilevalval
492549304935
glyasnmetthrprothrleuglyserleuserpheserhisgly
494049454950
gluglnarglysglyvalpheleutrpthrpheproserprogly
495549604965
trpproglualaphevalleuhisleuserglyvalglnserser
497049754980
alaproglyglyalaglnleuargserglypheilevalalaglu
498549904995
ilegluprometglyvalpheglnpheserthrserserargasn
500050055010
ileilevalsergluaspthrglnmetileargleuhisvalgln
501550205025
argleupheglyphehisseraspleuilelysvalsertyrgln
503050355040
thrthralaglyseralalysproleugluaspphegluproval
504550505055
glnasnglygluleuphepheglnlyspheglnthrgluvalasp
506050655070
phegluilethrileileasnaspglnleusergluilegluglu
507550805085
phephetyrileasnleuthrservalgluileargglyleugln
509050955100
lyspheaspvalasntrpserproargleuasnleuasppheser
510551105115
valalavalilethrileleuaspasnaspaspleualaglymet
512051255130
aspileserpheprogluthrthrvalalavalalavalaspthr
513551405145
thrleuileprovalgluthrgluserthrthrtyrleuserthr
515051555160
serlysthrthrthrileleuglnprothrasnvalvalalaile
516551705175
valthrglualathrglyvalseralaileproglulysleuval
518051855190
thrleuhisglythrproalavalserglulysproaspvalala
519552005205
thrvalthralaasnvalserilehisglythrpheserleugly
521052155220
proserilevaltyrileglugluglumetlysasnglythrphe
522552305235
asnthralagluvalleuileargargthrglyglyphethrgly
524052455250
asnvalserilethrvallysthrpheglygluargcysalagln
525552605265
metgluproasnalaleupropheargglyiletyrglyileser
527052755280
asnleuthrtrpalavalgluglugluasppheglugluglnthr
528552905295
leuthrleuilepheleuaspglygluarggluarglysvalser
530053055310
valglnileleuaspaspaspgluprogluglyglngluphephe
531553205325
tyrvalpheleuthrasnproglnglyglyalaglnilevalglu
533053355340
glulysaspaspthrglyphealaalaphealametvalileile
534553505355
thrglyseraspleuhisasnglyileileglyphesergluglu
536053655370
serglnserglyleugluleuarggluglyalavalmetargarg
537553805385
leuhisleuilevalthrargglnproasnargalaphegluasp
539053955400
vallysvalphetrpargvalthrleuasnlysthrvalvalval
540554105415
leuglnlysaspglyvalasnleuvalglugluleuglnserval
542054255430
serglythrthrthrcysthrmetglyglnthrlyscyspheile
543554405445
serilegluleulysproglulysvalproglnvalgluvaltyr
545054555460
phephevalgluleutyrglualathralaglyalaalaileasn
546554705475
asnseralaargphealaglnilelysileleugluseraspglu
548054855490
serglnserleuvaltyrpheservalglyserargleualaval
549555005505
alahislyslysalathrleuileserleuglnvalalaargasp
551055155520
serglythrglyleumetmetservalasnpheserthrglnglu
552555305535
leuargseralagluthrileglyargthrileileserproala
554055455550
ileserglylysaspphevalilethrgluglythrleuvalphe
555555605565
gluproglyglnargserthrvalleuaspvalileleuthrpro
557055755580
gluthrglyserleuasnserpheprolysargpheglnileval
558555905595
leupheaspprolysglyglyalaargileasplysvaltyrgly
560056055610
thralaasnilethrleuvalseraspalaaspserglnalaile
561556205625
trpglyleualaaspglnleuhisglnprovalasnaspaspile
563056355640
leuasnargvalleuhisthrilesermetlysvalalathrglu
564556505655
asnthraspgluglnleuseralametmethisleuileglulys
566056655670
ilethrthrgluglylysileglnalapheservalalaserarg
567556805685
thrleuphetyrgluileleucysserleuileasnprolysarg
569056955700
lysaspthrargglypheserhisphealagluvalthrgluasn
570557105715
phealapheserleuleuthrasnvalthrcysglyserprogly
572057255730
glulysserlysthrileleuaspsercysprotyrleuserile
573557405745
leualaleuhistrptyrproglnglnileasnglyhislysphe
575057555760
gluglylysgluglyasptyrileargileprogluargleuleu
576557705775
aspvalglnaspalagluilemetalaglylysserthrcyslys
578057855790
leuvalglnphethrglutyrserserglnglntrppheileser
579558005805
glyasnasnleuprothrleulysasnlysvalleuserleuser
581058155820
vallysglyglnserserglnleuleuthrasnaspasngluval
582558305835
leutyrargiletyralaalagluproargileileproglnthr
584058455850
serleucysleuleutrpasnglnalaalaalasertrpleuser
585558605865
aspserglnphecyslysvalvalglugluthralaasptyrval
587058755880
glucysalacysserhismetservaltyralavaltyralaarg
588558905895
thraspasnleusersertyrasnglualaphephethrsergly
590059055910
pheilecysileserglyleucysleualavalleuserhisile
591559205925
phecysalaargtyrsermetphealaalalysleuleuthrhis
593059355940
metmetalaalaserleuglythrglnileleupheleualaser
594559505955
alatyralaserproglnleualagluglusercysseralamet
596059655970
alaalavalthrhistyrleutyrleucysglnphesertrpmet
597559805985
leuileglnservalasnphetrptyrvalleuvalmetasnasp
599059956000
gluhisthrgluargargtyrleuleuphepheleuleusertrp
600560106015
glyleuproalaphevalvalileleuleuilevalileleulys
602060256030
glyiletyrhisglnsermetserglniletyrglyleuilehis
603560406045
glyaspleucyspheileproasnvaltyralaalaleuphethr
605060556060
alaalaleuvalproleuthrcysleuvalvalvalphevalval
606560706075
pheilehisalatyrglnvallysproglntrplysalatyrasp
608060856090
aspvalpheargglyargthrasnalaalagluileproleuile
609561006105
leutyrleuphealaleuileservalthrtrpleutrpglygly
611061156120
leuhismetalatyrarghisphetrpmetleuvalleupheval
612561306135
ilepheasnserleuglnglyleutyrvalphemetvaltyrphe
614061456150
ileleuhisasnglnmetcyscysprometlysalasertyrthr
615561606165
valglumetasnglyhisproglyproserthralaphephethr
617061756180
proglyserglymetproproalaglyglygluileserlysser
618561906195
thrglnasnleuileglyalametglugluvalproproasptrp
620062056210
gluargalaserpheglnglnglyserglnalaserproaspleu
621562206225
lysproserproglnasnglyalathrpheproserserglygly
623062356240
tyrglyglnglyserleuilealaaspglugluserglngluphe
624562506255
aspaspleuilephealaleulysthrglyalaglyleuserval
626062656270
seraspasngluserglyglnglyserglngluglyglythrleu
627562806285
thraspserglnilevalgluleuargargileproilealaasp
629062956300
thrhisleu
6305