一种大腹园蛛梨状腺丝蛋白的基因及其制备方法与流程

本发明属于基因及其制备领域，特别涉及一种大腹园蛛梨状腺丝蛋白的基因及其制备方法。

背景技术：

园蛛科蜘蛛可分泌7种蜘蛛丝，分别为主壶腹腺丝、次壶腹腺丝、鞭状腺丝、管状腺丝、梨状腺丝、葡萄状腺丝及聚合腺丝；蛛丝纤维由不同的蛛丝蛋白组成，具有不同的材料学性能和生物学功能。蜘蛛丝拥有优异的机械性能以及力学性能，是许多天然或人工材料无法比拟的。获得蛛丝蛋白基因的全长基因序列是研究蜘蛛丝性能及其机理的基础，蛛丝蛋白序列由相对保守的非重复区nt，ct以及一个很大的重复区组成，蛛丝蛋白往往是很大的蛋白质，相对分子量可达200-350kda。从dna文库中获取蛛丝全长基因耗时耗力，直接pcr得到全长蛛丝基因方便快捷，但由于缺乏相关目的序列片段及长距离pcr结果的不确定性,只有少数全长的蛛丝蛋白基因被克隆出来，严重制约了对蜘蛛丝的进一步研究。

梨状腺丝由梨状腺所分泌，属于固着丝，梨状腺丝其实是干性丝纤维和湿胶的复合体，主要用于蛛丝与固体支持物之间的连接。第一条pysp1全长基因在2017年才被报道出来，其包括21个重复单元，每个重复单元包括234个氨基酸残基，其中由包含大量px和qq小模块。

蜘蛛丝外观光滑纤巧、丝滑闪亮、坚韧轻柔，且具有生物相容性以及较好的记忆性能，作为性能优异的天然蛋白类高分子材料具有极大的潜在应用价值，人工制备的蛛丝蛋白通过不同的方法可以制备成不同的形态，如纤维、膜、微球、胶囊等作为生物材料应用于军事、航天及生物医学领域，且其蛋白成分决定了它的可降解性，符合可持续发展的战略要求。

技术实现要素：

本发明所要解决的技术问题是提供一种大腹园蛛梨状腺丝蛋白的基因及其制备方法，克服了现有技术的大腹园蛛梨状腺丝蛋白基因空白的缺陷，该方法克隆了整个基因包含有完整的nt端，重复区以及完整的ct端，用于编码大腹园蛛梨状腺丝蛋白；而且对环境无污染；制备方法工艺简单，条件温和，易于操作，为在基因工程上大规模生产蛛蛋白奠定了基因基础。

本发明的一种大腹园蛛梨状腺丝蛋白的基因，所述基因序列如seqidno.1所示。

所述基因全长大小为11,931bp，共编码3976个氨基酸，具体序列如seqidno.1所示，蛋白分子量为400.0kda。

所述全长基因中nt端大小为450bp，编码150个氨基酸，具体序列如seqidno.2所示。

n-linker区大小为768bp，共编码256个氨基酸，具体序列如seqidno.3所示。

重复区大小为10,116bp，共编码3372个氨基酸，具体序列如seqidno.4所示。

c-linker区大小为327bp，共编码109个氨基酸，具体序列如seqidno.5所示

ct端大小为267bp，共编码89个氨基酸，具体序列如seqidno.6所示。

本发明一种大腹园蛛梨状腺丝蛋白的基因的制备方法，包括：

(1)根据蜘蛛梨状腺丝的pysp1蛋白基因，在nt端简并引物，在重复区设计2条特异性引物，通过简并pcr扩增得到部分的nt端基因序列；

(2)根据步骤(1)所获得的序列分别设计2条特异性引物及1条锚定引物，通过锚定pcr将nt端补齐；

(3)根据步骤(2)所获得的包含完整nt端序列，在nt端5’末端设计1条正向引物，在ct端3’末端设计1条反向引物，用于扩增全长pysp1基因；

(4)对步骤(3)所得pcr产物进行琼脂糖凝胶切胶回收后，与载体进行平末端连接反应，之后进行连接产物回收；

(5)对步骤(4)的连接产物进行平末端克隆，使用热击转化法，转入dh5α感受态细胞中进行转化，复苏时间1h，将转化后的菌液涂布在lb固体培养基上，过夜培养后，挑取单克隆进行pcr检测；

(6)将步骤(5)中得到的阳性单克隆的质粒进行完整测序，最终得到大腹园蛛梨状腺丝蛋白全长基因。

上述制备方法的优选方式如下：

所述步骤(1)中用于扩增部分nt端的引物：正向简并引物：atgttycaratgagttcaatg；

如seqidno.7所示。

反向重复区特异性引物：ctgttgtgtttgagcggattg。如seqidno.8所示。所述步骤(1)中简并pcr扩增的条件为：95℃预变性5min，95℃变性30s，55℃退火30s，72℃延伸30s，循环30次。

所述步骤(2)中用于补全nt端的特异性引物：

引物1：tttcaatagctgccgcctgc；如seqidno.9所示。

引物2：gtggcaatagcttgggttacgc；如seqidno.10所示。

锚定引物：actcctgtggaaccatcggacgggggg。如seqidno.11所示。

所述步骤(2)中锚定pcr条件为：在第一轮pcr中，使用特异性引物1进行单引物扩增，条件：95℃预变性5min，95℃变性30s，60℃退火30s，72℃延伸30s，循环30次；之后将pcr产物进行琼脂糖凝胶切胶回收，用tdt酶进行加多聚dctp反应，产物回收后进行第二轮pcr；使用特异性引物2以及锚定引物进行扩增，条件同上。

所述步骤(3)中用于扩增全长pysp1基因的引物：

正向引物：atgtcttggaccctggggcttcagtttttattc；如seqidno.12所示

反向引物：ctatccaagtgctgcaagtacgac。如seqidno.13所示。

1对引物分别位于pysp基因的5’末端以及3’末端，以获得完整的pysp1基因。

所述步骤(3)中用于扩增全长pysp1基因的酶为phanta高保真聚合酶。

所述步骤(3)中扩增条件为：98℃预变性1min，98℃变性5s，60℃退火15s，72℃延伸10min，循环30次。

所述步骤(4)中载体为：peasy-bluntzerocloningvector。

所述步骤(4)中平末端连接反应为：16℃反应过夜。

所述步骤(5)中连接产物和dh5α感受态细胞的体积比为1:10；lb固体培养基含有氨苄青霉素amp和卡那霉素kan，氨苄青霉素和卡那霉素在培养基中的浓度均为100μg/ml。

本发明的一种大腹园蛛梨状腺丝蛋白的基因的应用，如作为纤维材料。

大腹园蛛梨状腺丝蛋白的基因的功能为：梨状腺丝蛋白是最近才被发现的一种蛛丝蛋白，它是组成附着盘的主要蛋白。附着盘用于连接网的交界处、附着拖丝到其它介质的表面，而且也被用来捕捉和移动猎物。扫描电子显微镜研究发现：在附着盘中，小直径蛋白纤维被埋在像胶的粘合物中，形成类似于树根形状的网络，而且里面还有大直径拖丝，是一种黏性极强的材料，在蜘蛛生命活动中扮演重要角色。

有益效果

本发明首先通过简并pcr扩增出pysp1的nt端部分序列，然后利用锚定pcr将其补全，之后采用长距离pcr方法扩增得到完整的pysp1基因并进行平末端克隆，得到含有全长pysp1基因的克隆；

本发明克隆了整个基因包含有完整的nt端，重复区以及完整的ct端，用于编码大腹园蛛梨状腺丝蛋白；而且对环境无污染；

首次尝试构建具有天然大腹园蛛梨状腺丝蛋白主要特征模块的迷你型蛛丝蛋白基因，(av.pyspnt-pysprp-pyspct)及pysp的一个完整的重复区域(av.pysprp)，但迷你型pysp蛛丝蛋白基因av.pyspnt-pysprp-pyspct在e.colibl21(de3)菌株中的表达量极低，单纯pysp完整的重复区(av.pysprp)基因在e.colibl21(de3)菌株中可以正常表达，得到的大腹园蛛梨状腺丝蛋白，经过静电纺丝可将该蛋白纺成纳米纤维膜；

本发明制备方法工艺简单，条件温和，易于操作，为在基因工程上大规模生产蛛蛋白奠定了基因基础。

附图说明

图1为本发明大腹园蛛pysp1蛋白的氨基酸序列。

图2为本发明大腹园蛛pysp1蛋白的疏水性分析图；其中图2上为完整的pysp1蛋白疏水性图，图2下自左至右依次为nt端、n-linker、repeat8、ct端的疏水性图。

图3为本发明大腹园蛛pysp1蛋白的二级结构预测图；其中图3自左至右依次为nt端、repeat8、ct端的二级结构预测图。

图4为本发明大腹园蛛pysp1蛋白的序列比对图；其中，图4a为5种pysp1蛋白的nt端序列比对图，图4b为4种pysp1蛋白的重复单元序列比对图，图4c为7种pysp1蛋白的ct端序列比对图。

图5为纯pysp蛋白纤维膜的sem照片(1000×)。

具体实施方式

下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。此外应理解，在阅读了本发明讲授的内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。

实施例1

(1)根据其它蜘蛛的pysp1的nt端的保守序列，分别在nt端和ct端各设计1条简并引物：nt端简并引物：atgttycaratgagttcaatg，并在重复区设计1条特异性反向引物，特异性反向引物：ctgttgtgtttgagcggattg；然后进行pcr，条件为95℃预变性5min，95℃变性30s，55℃退火30s，72℃延伸30s，循环30次。

(2)根据所获得的序列分别在nt端和ct端各设计2条特异性引物以及1条锚定引物用于补全nt端和ct端。

nt端特异性引物1：tttcaatagctgccgcctgc；

nt端特异性引物2：gtggcaatagcttgggttacgc；

:锚定引物：actcctgtggaaccatcggacgggggg；

锚定pcr条件为：在第一轮pcr中，使用特异性引物1进行单引物扩增，条件：95℃预变性5min，95℃变性30s，60℃退火30s，72℃延伸30s，循环30次。之后将pcr产物进行琼脂糖凝胶切胶回收，用tdt酶进行加c反应，产物回收后进行第二轮pcr。使用特异性引物2以及锚定引物进行扩增，条件同上。

(3)之后设计1对特异性引物用于扩增全长pysp基因片段，

正向引物：atgtcttggaccctggggcttcagtttttattc；

反向引物：ctatccaagtgctgcaagtacgac；

pcr条件为98℃预变性1min，98℃变性5s，60℃退火15s，72℃延伸10min，循环30次。之后琼脂糖凝胶切胶回收。(phanta高保真聚合酶添加在pcr反应液中)

(4)将回收产物与载体peasy-bluntzerocloningvector进行平末端连接反应，条件为：16℃反应过夜。

(5)之后转入dh5α感受态细胞，体积比为1：10，复苏时间为1h。之后将菌液涂布在氨苄青霉素和卡那霉素的固体培养基上，浓度均为100μg/ml，37℃培养12h。然后对菌落进行筛选，测序。

使用signalp4.1、expasytools、psipred等在线软件以及geneious、dnastar软件对该基因进行分析，signalp4.1用于寻找基因的信号肽，expasytools用来对pysp1的疏水性进行分析，psipred对pysp1的二级结构进行预测，geneious用来进行多序列比对，dnastar用来对pysp1基因的碱基组成和氨基酸组成进行分析。

分析结果如下：

signalp4.1以及dnastar分析结果：

图1为pysp1蛋白的结构域以及序列图谱，从图1中可以看出，整个pysp1基因划分为五个区域，nt端，n-linker，重复区，c-linker以及ct端。其中重复区内由16个在序列上极其相似的重复单元组成，并占据了整个蛋白序列的85％以上。其中前15个重复单元大小为213个氨基酸，第16个重复单元为177个氨基酸。在图1中可以发现蛋白的信号肽存在于nt端中(粗体标记)，并且存在2个cys残基。

本发明大腹园蛛pysp1蛋白的氨基酸序列，同时如seqidno.16所示。

expasytools分析结果：图2为整个pysp1蛋白的疏水性分析图以及nt端，repeatunit8(r8)，ct端的疏水性图。从图2中可以看到，疏水性区域以及亲水性区域在整个蛋白序列中反复变化，整个蛋白分子表现为疏水性。ct端的平均疏水性值大于nt端，但nt端的疏水性峰值大于ct端。

psipred分析结果：图3为pysp蛋白二级结构预测图，从图3可以看出，在nt端中含有5个α螺旋结构，第一个cys残基处于第1个和第2个螺旋之间，第二个cys残基处于第4个螺旋中。在图3中可以发现ct端中只含有4个螺旋结构。图3中重复单元内有8个螺旋结构。

geneious分析结果：图4为pysp蛋白的对比图。从图4a，图4b，图4c中可以看出不同物种之间的pysp的nt端，重复单元以及ct端都有较高的相似度。

实施例2

1.根据pysp重复区序列设计引物

primer1：tattctgcagcatcaactgcc；如seqidno.14所示

primer2：agcgtttgagctttgttgagattg；如seqidno.15所示。

扩增条件为：95℃预变性5，95℃变性30s，57℃退火30s，72℃延伸1min，循环30次。

扩增全长pysp重复区基因片段得到pcr产物。将上述实验制备所得到的插入片段和pehs载体进行连接，在冰盒上解冻以下试剂，并按下列顺序在冰上加样，连接体系如下：

插入片段7l

pehs载体1l

t4dnaligase1l

10×t4ligationbuffer1l

total10l

置于pcr管中，混匀，于室温下连接6h。连接产物热激转化入e.colidh5α感受态细胞。

抽取质粒转入bl21菌株，进行蛋白诱导表达：

(1)蛋白诱导表达5h后，使用低温高速离心机4000rpm，15min离心收集菌体，并用20mm的tris-hcl(ph8.0)以30ml/l菌液的比例溶解；

(2)将溶解得到的混合液使用机械细胞破碎机进行细胞破碎，菌体破碎后12000rpm，4℃，10min收集包涵体；

(3)将离心得到的包涵体全部溶于8m的尿素中，按照70ml/l的比例，将混合液套上保鲜膜于电磁加热搅拌器上孵育30～60min；

(4)孵育后的混合液，12000rpm，25℃条件下离心30min；

(5)离心后，收集上清液，取出部分使用sds-page检测蛋白表达的裂解的情况；

(6)剪一段合适长度的透析袋，用去离子水将透析袋内部冲洗干净，待用；

(7)将透析袋的一端用透析夹夹紧，倒入蛋白液之前可先向其中加入少量去离子水以检查密封性；

(8)将处理过的含尿素的蛋白溶液缓慢倒入透析袋中，最后，将透析袋的另一端同样用透析夹夹紧；

(9)将透析袋放入装有超纯水的大的容器中，放入转子并置于电磁搅拌器上搅拌，约2h更换一次容器中的透析液，整个透析过程进行约48h。

透析结束后，将透析袋内的蛋白液全部倒入到500ml冻干杯内，用冷冻干燥机冻干，至杯中出现粉状的蛋白。取蛋白粉进行静电纺：

(1)取0.24g提取的蛋白加入3ml的六氟异丙醇(hfip)制成8％的纺丝液；

(2)另取一干净的针管，将混合均匀的纺丝液倒入针管中，并将处理好的针头插好，固定在注射棒上；

(3)准备一张约17cm*17cm的正方形纸板，用铝箔纸包好，使一面光滑完整；

(4)设置注射棒的参数，5cc，0.80ml/h，4.00ml；

(5)待针头处有液滴流出时，接通高压电源(16kv)，开始静电纺丝，可以观察到，在电场力的作用下，枕头出的液滴由球型变为圆锥形(taylor锥)；

(6)静电纺丝结束后，关闭电源，将用过的针头取下重新置于六氟异丙醇中循环使用，纺得的纳米纤维膜放于干燥处储存待用。

将纺得的膜于扫描电子显微镜(sem)下拍照，参见附图5。

由此可以说明，利用纯pysp蛋白进行静电纺丝制备纳米纤维膜是可行的，为蛛丝纤维应用提供了基础材料。

sequencelisting

<110>东华大学

<120>一种大腹园蛛梨状腺丝蛋白的基因及其制备方法

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<160>16

<170>patentinversion3.3

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atgtcttggaccctggggcttcagtttttattcacattttgcttggtgctcttcgcatgg60

aggggagatgctctttcagacccaaaatcttgggttcaggacgaagcgacggccgtttat120

ttcttggagcgcaccttggagtatctgggagaatgcggcgttctgaaaattgaccaggag180

agagatgccatcgacaccatgtttcagatgagttcaatgtttcaacagaatgcaaaaccc240

agcaagataaagcactctatatcttccaagttggccgacatagtcatagaaggtctggag300

ggagacagcgacccgtcctacaagatcgactgcgtaacccaagctattgccaccgctttc360

gaagcaacaacaggtgttcgagatgaaaatttcattgagtctgtcattgaacttatagta420

ttaatatttaacaatgatatcgaagagaagatggaagaagaaatcgaagagactgctgca480

agtcagcaggcggcagctattgaaaaccaaatttcgacaatagaatcgaactttggatcg540

gcacagactcagtcatcagtgcagaacgatatacagcagcaaagcactgtagatatttct600

tcttcggtcaccaatcagattcagtctcagcagtatgcagacagtcaatcttccgcgcaa660

attcagtcacagcaaagtgtcgctagccaacaacaagatcttacttctcaactaacgact720

tctagcgctcagcaacaatatgaaacttcccaagcttctatcgcacagcagcaatatgaa780

acttcccaagcttctatcgcgcagcagcaatatgaagcttctcaacagcaggcttctatc840

gagcagcaacaatatgaagcttcgcaagcttctatcgcgcaacagcaatatgaagcttct900

caacagcaggcttctatcgagcagcaacaatatgaagcttcgcaagcttctatcgcgcaa960

cagcaatatgaagcttctcaacagcaagcttctatcgcgcagcaacaatatcaaagttcg1020

cagcaacaggcttctataaatcagcaacaatatgaagcttctcagcagcaatcttccagt1080

tctgaacagcagtacacaacttccgaacagcaaattcagcagtccgagaatgcatatacc1140

gatcagtcaaatattcaacaatccgctcaaacacaacagtacagcacttcacaacaagaa1200

caagaatcctctgcaagctattctacagcctcaattgccggttcaagtgtgtcgcaatct1260

caggcgactgtctcaagcgcacctgattattttaacacgcaaactttaagtagcagcctg1320

tcttcttctctgcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcc1380

tcgaatgccgcttctattttagcgagtgcagtggcacagtcacttggagtgtcccaagcg1440

tcggttcaaaatagcataagccaacagttgagaagcgtagggcccggatcttccacgtcc1500

tctgtcgctcaagcaatagcaaatggagtggctaacgcagttggagcatcaggaactgga1560

gttgcaggacaagaacaatctatttcacaatccatatatacttcaatttccactgctctt1620

tctcaactggcagcaccggctccagcacctgcaccgagacttgctcctagaccactacca1680

gccccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtttacgcacca1740

gccccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagt1800

gccttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttat1860

tctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgct1920

cctgtttattttaacacgcaaactttaagtagcagcctgtcttcttctctgcaatcactc1980

agtgcactcaattcgttagcgagtggtcaactgagctcctggaatgccgcttctattata2040

gcgagtgcggtagcaccgtcacttggagtgtcccaagcgtcggttcaaaatagtataagc2100

caacagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagca2160

aatggagtggctaacgcagttggagcatcaggaacaggagttgcaggacaagaacaatct2220

atttcacaatccatatatacttcagtttccactgctctatctcaactggcagcaccggct2280

ccagcacctgcacctagaccagctcctcgaccactaccagccccaattcaagccccaaga2340

ccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagtt2400

caggcaacttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaa2460

tcttcagttgttcaatctcaacaaagctcaaacgcttattctgcagcttcatctgtaggc2520

tcaagtttttcgcagtctcaggggactgtcccaagcgctcctgtttattttaacacgcaa2580

actttaagtagcagcctgtcttcttctctgcaatcactcagtgcactcaattcgatagcg2640

agtggtcaactgagctcctcgaatgccgcttctattatagcgagtgcagtggcacggtca2700

cttggagtgtcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtaggg2760

cccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagtt2820

ggagcatcaggaactggagttgcaggacaagaacaatctatttcacaatccatatatact2880

tcaatttccactgctctttctcaactggcagcaccggctccagcacctgcaccgagactt2940

gctcctagaccactaccagccccaattcaagccccaagaccagcacccgcaccacaacct3000

gcaccggtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaa3060

gcttcggctcaacagagtgccttcgcacagtcccagcaatcttcagttgcacaatctcaa3120

caaagctcaaacgttgattctgcagcttcatctgtaggctcaagtttttcgcagtctcag3180

gggactgtcccaagcgctcctgtttattttaacacgcaaactttaagtagcagcctgtct3240

tcttctctgcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcatcg3300

tatgccgattctattttagcgagtgcagtggctcggtctcttggagtttcccaagcgtcg3360

gttctaaatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcctct3420

gtcgctcaagcaatagcaaatggagtggctaacgcagttggagcatcaggaacaggagtt3480

gcaggacaagaacaatctatttcacaatccatatatacttcagtttccactgctctttct3540

caactggcagcaccggctccagcacctgcaccgagacttgctcctagaccactaccagcc3600

ccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagcc3660

ccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgcc3720

ttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattct3780

gcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcct3840

gattattttaacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagt3900

gcactcaattcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcg3960

agtgcagtggcacggtcacttgtagtgtcccacgcgtcggttcaaaatagtataagccaa4020

cagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaat4080

ggagtggctaacgcagttggagcatcaggaactggagttgcaggacaagaacaatctatt4140

tcacaatccatatatacttcaatttccactgctctttctcaactggcagcaccggctcca4200

gcacctgcaccgagacttgctcctagaccactaccagccccaattcaagccccaagacca4260

gcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcag4320

gcaacttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatct4380

tcagttgttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttca4440

agtgtgtcgcaatctcaggcgattgtctcaagcgctcctgattattttaacacgcaaact4500

ttaagtagcagcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcgagt4560

ggtcaactgagctcctcgaatgccgcttctattttagcgagtgcagtggcacggtcactt4620

ggagtgtcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggccc4680

ggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagttgga4740

gcatcaggaactggagttgcaggacaagaacaatctatttcacaatccatatatacttca4800

atttccactgctctttctcaactggcagcaccggctccagcacctgcaccgagacttgct4860

cctagaccactaccagccccaattcaagccccaagaccagcacccgcaccacaacctgca4920

ccggtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcc4980

tcggctcaacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaa5040

agttcaaacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcg5100

attgtctcaagcgctcctgattattttaacacgcaaactttaagtagcagcctgtcttct5160

tctcagcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaat5220

gccgcttctattttagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggtt5280

caaaatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtc5340

gctcaagcaatagcaaatggagtggctaacgcagttggagcatcaggaactggagttgca5400

ggacaagaacaatctatttcacaatccatatatacttcaatttccactgctctttctcaa5460

ctggcagcaccggctccagcacctgcaccgagacttgctcctagaccactaccagcccca5520

attcaagccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagcccca5580

gtcgtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgccttc5640

gcacagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattctgca5700

gcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgat5760

tattttaacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagtgca5820

ctcaattcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcgagt5880

gcagtggcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacag5940

ttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatgga6000

gtggctaacgcagttggagcatcaggaactggagttgcaggacaagaacaatctatttca6060

caatccatatatacttcaatttccactgctctttctcaactggcagcaccggctccagca6120

cctgcaccgagacttgctcctagaccactaccagccccaattcaagccccaagaccagca6180

cccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcaggca6240

acttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatcttca6300

gttgttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttcaagt6360

gtgtcgcaatctcaggcgattgtctcaagcgctcctgattattttaacacgcaaacttta6420

agtagcagcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcgagtggt6480

caactgagctcctcgaatgccgcttctattttagcgagtgcagtggcacggtcacttgga6540

gtgtcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggcccgga6600

tcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagttggagca6660

tcaggaactggagttgcaggacaagaacaatctatttcacaatccatatatacttcaatt6720

tccactgctctttctcaactggcagcaccggctccagcacctgcaccgagacttgctcct6780

agaccactaccagccccaattcaagccccaagaccagcacccgcaccacaacctgcaccg6840

gtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcg6900

gctcaacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagt6960

tcaaacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgatt7020

gtctcaagcgctcctgattattttaacacgcaaactttaagtagcagcctgtcttcttct7080

cagcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaatgcc7140

gcttctattttagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggttcaa7200

aatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtcgct7260

caagcaatagcaaatggagtggctaacgcagttggagcatcaggaacaggagttgcagga7320

caagaacaatctatttcacaatccatagatacttcaatttccactgctctttctcaactg7380

gcagcaccggctccagcacctgcaccgagacttgctcctagaccactaccagccccaatt7440

caagccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtc7500

gtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgccttcgca7560

cagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattctgcagca7620

tcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgattat7680

tttaacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagtgcactc7740

aattcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcgagtgca7800

gtggcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacagttg7860

agaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtg7920

gctaacgcagttggagcatcaggaactggagttgcaggacaagaacaatctatttcacaa7980

tccatatatacttcaatttccactgctctttctcaactggcagcaccggctccagcacct8040

gcaccgagacttgctcctagaccactaccagccccaattcaagccccaagaccagcaccc8100

gcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcaggcaact8160

tcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatcttcagtt8220

gttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttcaagtgtg8280

tcgcaatctcaggcgattgtctcaagcgctcctgtttattttaacacgcaaactttaagt8340

agcagcctgtcttcttctctgcagtcactcagtgcactcaattcgatagcgagtggtcaa8400

ctgagctcctcgaatgccgcttctattatagcgagtgcagtggcacggtcacttggagtg8460

tcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggcccggatct8520

tccacgtcctctgtcgatcaagcaatagcaaatgtagaggctaacgcagatggatcatca8580

ggaacaggagttgcaggacaagaacaatctatttcacaatccatatatacttcaatttcc8640

actgctctttctcaactggcagcaccggctccagcacctgcaccgagacttgctcctaga8700

ccactaccagccccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtt8760

tacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcggct8820

caacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagttca8880

aacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtc8940

tcaagcgctcctgattattttaacacgcaaactttaagtagcagcctgtcttcttctcag9000

caatcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaatgccgct9060

tctattttagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggttcaaaat9120

agtataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaa9180

gcaatagcaaatggagtggctaacgcagttggagcatcaggaactggagttgcaggacaa9240

gaacaatctatttcacaatccatatatacttcaatttccactgctctttctcaactggca9300

gcaccggctccagcacctgcaccgagacttgctcctagaccactaccagccccaattcaa9360

gccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtt9420

tcacaagttcaggcaacttcttcttctcaagcctcggctcaacagagtgccttcgcacag9480

tcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattctgcagcatca9540

actgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgattatttt9600

aacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagtgcactcaat9660

tcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcgagtgcagtg9720

gcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacagttgaga9780

agcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggct9840

aacgcagttggagcatcaggaactggagttgcaggacaagaacaatctatttcacaatcc9900

atatatacttcaatttccactgctctttctcaactggcagcaccggctccagcacctgca9960

ccgagacttgctcctagaccactaccagccccaattcaagccccaagaccagcacccgca10020

ccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcaggcaacttct10080

tcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatcttcagttgtt10140

caatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttcaagtgtgtcg10200

caatctcaggcgattgtctcaagcgctcctgattattttaacacgcaaactttaagtagc10260

agcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcgagtggtcaactg10320

agctcctcgaatgccgcttctattttagcgagtgcagtggcacggtcacttggagtgtcc10380

caagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggcccggatcttcc10440

acgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagttggagcatcagga10500

actggagttgcaggacaagaacaatctatttcacaatccatatatacttcaatttccact10560

gctctttctcaactggcagcaccggctccagcacctgcacctagaccagctcctcgacca10620

ctaccagccccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtttac10680

gcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcggctcaa10740

cagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagttcaaac10800

gcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctca10860

agcgctcctgtttattttaacacgcaaactttaagtagcagcctgtcttcttctctgcaa10920

tcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaatgccgcttct10980

attatagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggttcaaaatagt11040

ataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagca11100

atagcaaatggagtggctaacgcagttggagcatcaggaacaggagttgcaggacaagaa11160

caatctatttcacaatccatatatacttcaatttccactgctctttctcaactggcagca11220

acggctccagcacctgcaccgagaccagctcctcgaccactaccagccccaattcaagcc11280

ccaagaccagcacccgcaccacaacctgcgccagtttacgcacccgcgccagtgtacgct11340

ccagcgccagtgtacgctccagcgccaatcgtttcccagattcaggaaacttcttcttct11400

caagcctcggcgcaacagaattatttggcacagtcccagcaatcttccgttgctcaatct11460

caacaaagttcgaacgcttattcggcagtatcagctgctgattcctcattttcaagcgga11520

gcttcatcaggatttggctcttctctgtcttcttcctctggcttttctgccccagccagc11580

cccgcgccgtattctccctcagtttccgcaagttcttcgattggagcctcgtccggatct11640

gcgttggccgcttctgccgcccagcaactcatgtctcccgcagcgaaccaaaggattgcg11700

gctctgtccaattctctgagggctgctgtatccggaggacaagtcaactatggcgccctc11760

tccaactcgcttgccagcgcagcgagtcagatacagagttcatccggcttgtccaagagt11820

gaagttttagtcgaggctctcctggaaacgttgtttgctctgctgagctctctcacgtct11880

ggagcgtctacggctgatttcgcacaggtcgtacttgcagcacttggatag11931

<210>2

<211>450

<212>dna

<213>人工序列

<400>2

atgtcttggaccctggggcttcagtttttattcacattttgcttggtgctcttcgcatgg60

aggggagatgctctttcagacccaaaatcttgggttcaggacgaagcgacggccgtttat120

ttcttggagcgcaccttggagtatctgggagaatgcggcgttctgaaaattgaccaggag180

agagatgccatcgacaccatgtttcagatgagttcaatgtttcaacagaatgcaaaaccc240

agcaagataaagcactctatatcttccaagttggccgacatagtcatagaaggtctggag300

ggagacagcgacccgtcctacaagatcgactgcgtaacccaagctattgccaccgctttc360

gaagcaacaacaggtgttcgagatgaaaatttcattgagtctgtcattgaacttatagta420

ttaatatttaacaatgatatcgaagagaag450

<210>3

<211>768

<212>dna

<213>人工序列

<400>3

atggaagaagaaatcgaagagactgctgcaagtcagcaggcggcagctattgaaaaccaa60

atttcgacaatagaatcgaactttggatcggcacagactcagtcatcagtgcagaacgat120

atacagcagcaaagcactgtagatatttcttcttcggtcaccaatcagattcagtctcag180

cagtatgcagacagtcaatcttccgcgcaaattcagtcacagcaaagtgtcgctagccaa240

caacaagatcttacttctcaactaacgacttctagcgctcagcaacaatatgaaacttcc300

caagcttctatcgcacagcagcaatatgaaacttcccaagcttctatcgcgcagcagcaa360

tatgaagcttctcaacagcaggcttctatcgagcagcaacaatatgaagcttcgcaagct420

tctatcgcgcaacagcaatatgaagcttctcaacagcaggcttctatcgagcagcaacaa480

tatgaagcttcgcaagcttctatcgcgcaacagcaatatgaagcttctcaacagcaagct540

tctatcgcgcagcaacaatatcaaagttcgcagcaacaggcttctataaatcagcaacaa600

tatgaagcttctcagcagcaatcttccagttctgaacagcagtacacaacttccgaacag660

caaattcagcagtccgagaatgcatataccgatcagtcaaatattcaacaatccgctcaa720

acacaacagtacagcacttcacaacaagaacaagaatcctctgcaagc768

<210>4

<211>10116

<212>dna

<213>人工序列

<400>4

tattctacagcctcaattgccggttcaagtgtgtcgcaatctcaggcgactgtctcaagc60

gcacctgattattttaacacgcaaactttaagtagcagcctgtcttcttctctgcaatca120

ctcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaatgccgcttctatt180

ttagcgagtgcagtggcacagtcacttggagtgtcccaagcgtcggttcaaaatagcata240

agccaacagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaata300

gcaaatggagtggctaacgcagttggagcatcaggaactggagttgcaggacaagaacaa360

tctatttcacaatccatatatacttcaatttccactgctctttctcaactggcagcaccg420

gctccagcacctgcaccgagacttgctcctagaccactaccagccccaattcaagcccca480

agaccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaa540

gttcaggcaacttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaa600

caatcttcagttgttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgcc660

ggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgtttattttaacacg720

caaactttaagtagcagcctgtcttcttctctgcaatcactcagtgcactcaattcgtta780

gcgagtggtcaactgagctcctggaatgccgcttctattatagcgagtgcggtagcaccg840

tcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgta900

gggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgca960

gttggagcatcaggaacaggagttgcaggacaagaacaatctatttcacaatccatatat1020

acttcagtttccactgctctatctcaactggcagcaccggctccagcacctgcacctaga1080

ccagctcctcgaccactaccagccccaattcaagccccaagaccagcacccgcaccacaa1140

cctgcaccggtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctct1200

caagcctcggctcaacagagtgccttcgcacagtcccaacaatcttcagttgttcaatct1260

caacaaagctcaaacgcttattctgcagcttcatctgtaggctcaagtttttcgcagtct1320

caggggactgtcccaagcgctcctgtttattttaacacgcaaactttaagtagcagcctg1380

tcttcttctctgcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcc1440

tcgaatgccgcttctattatagcgagtgcagtggcacggtcacttggagtgtcccaagcg1500

tcggttcaaaatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcc1560

tctgtcgctcaagcaatagcaaatggagtggctaacgcagttggagcatcaggaactgga1620

gttgcaggacaagaacaatctatttcacaatccatatatacttcaatttccactgctctt1680

tctcaactggcagcaccggctccagcacctgcaccgagacttgctcctagaccactacca1740

gccccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtttacgcacca1800

gccccagtcgtttcacaagttcaggcaacttcttcctctcaagcttcggctcaacagagt1860

gccttcgcacagtcccagcaatcttcagttgcacaatctcaacaaagctcaaacgttgat1920

tctgcagcttcatctgtaggctcaagtttttcgcagtctcaggggactgtcccaagcgct1980

cctgtttattttaacacgcaaactttaagtagcagcctgtcttcttctctgcaatcactc2040

agtgcactcaattcgatagcgagtggtcaactgagctcatcgtatgccgattctatttta2100

gcgagtgcagtggctcggtctcttggagtttcccaagcgtcggttctaaatagtataagc2160

caacagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagca2220

aatggagtggctaacgcagttggagcatcaggaacaggagttgcaggacaagaacaatct2280

atttcacaatccatatatacttcagtttccactgctctttctcaactggcagcaccggct2340

ccagcacctgcaccgagacttgctcctagaccactaccagccccaattcaagccccaaga2400

ccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagtt2460

caggcaacttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaa2520

tcttcagttgttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggt2580

tcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgattattttaacacgcaa2640

actttaagtagcagcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcg2700

agtggtcaactgagctcctcgaatgccgcttctattttagcgagtgcagtggcacggtca2760

cttgtagtgtcccacgcgtcggttcaaaatagtataagccaacagttgagaagcgtaggg2820

cccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagtt2880

ggagcatcaggaactggagttgcaggacaagaacaatctatttcacaatccatatatact2940

tcaatttccactgctctttctcaactggcagcaccggctccagcacctgcaccgagactt3000

gctcctagaccactaccagccccaattcaagccccaagaccagcacccgcaccacaacct3060

gcaccggtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaa3120

gcctcggctcaacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaa3180

caaagttcaaacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcag3240

gcgattgtctcaagcgctcctgattattttaacacgcaaactttaagtagcagcctgtct3300

tcttctcagcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcg3360

aatgccgcttctattttagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcg3420

gttcaaaatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcctct3480

gtcgctcaagcaatagcaaatggagtggctaacgcagttggagcatcaggaactggagtt3540

gcaggacaagaacaatctatttcacaatccatatatacttcaatttccactgctctttct3600

caactggcagcaccggctccagcacctgcaccgagacttgctcctagaccactaccagcc3660

ccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagcc3720

ccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgcc3780

ttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattct3840

gcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcct3900

gattattttaacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagt3960

gcactcaattcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcg4020

agtgcagtggcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaa4080

cagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaat4140

ggagtggctaacgcagttggagcatcaggaactggagttgcaggacaagaacaatctatt4200

tcacaatccatatatacttcaatttccactgctctttctcaactggcagcaccggctcca4260

gcacctgcaccgagacttgctcctagaccactaccagccccaattcaagccccaagacca4320

gcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcag4380

gcaacttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatct4440

tcagttgttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttca4500

agtgtgtcgcaatctcaggcgattgtctcaagcgctcctgattattttaacacgcaaact4560

ttaagtagcagcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcgagt4620

ggtcaactgagctcctcgaatgccgcttctattttagcgagtgcagtggcacggtcactt4680

ggagtgtcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggccc4740

ggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagttgga4800

gcatcaggaactggagttgcaggacaagaacaatctatttcacaatccatatatacttca4860

atttccactgctctttctcaactggcagcaccggctccagcacctgcaccgagacttgct4920

cctagaccactaccagccccaattcaagccccaagaccagcacccgcaccacaacctgca4980

ccggtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcc5040

tcggctcaacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaa5100

agttcaaacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcg5160

attgtctcaagcgctcctgattattttaacacgcaaactttaagtagcagcctgtcttct5220

tctcagcaatcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaat5280

gccgcttctattttagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggtt5340

caaaatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtc5400

gctcaagcaatagcaaatggagtggctaacgcagttggagcatcaggaactggagttgca5460

ggacaagaacaatctatttcacaatccatatatacttcaatttccactgctctttctcaa5520

ctggcagcaccggctccagcacctgcaccgagacttgctcctagaccactaccagcccca5580

attcaagccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagcccca5640

gtcgtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgccttc5700

gcacagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattctgca5760

gcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgat5820

tattttaacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagtgca5880

ctcaattcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcgagt5940

gcagtggcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacag6000

ttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatgga6060

gtggctaacgcagttggagcatcaggaacaggagttgcaggacaagaacaatctatttca6120

caatccatagatacttcaatttccactgctctttctcaactggcagcaccggctccagca6180

cctgcaccgagacttgctcctagaccactaccagccccaattcaagccccaagaccagca6240

cccgcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcaggca6300

acttcttcctctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatcttca6360

gttgttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttcaagt6420

gtgtcgcaatctcaggcgattgtctcaagcgctcctgattattttaacacgcaaacttta6480

agtagcagcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcgagtggt6540

caactgagctcctcgaatgccgcttctattttagcgagtgcagtggcacggtcacttgga6600

gtgtcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggcccgga6660

tcttccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagttggagca6720

tcaggaactggagttgcaggacaagaacaatctatttcacaatccatatatacttcaatt6780

tccactgctctttctcaactggcagcaccggctccagcacctgcaccgagacttgctcct6840

agaccactaccagccccaattcaagccccaagaccagcacccgcaccacaacctgcaccg6900

gtttacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcg6960

gctcaacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagt7020

tcaaacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgatt7080

gtctcaagcgctcctgtttattttaacacgcaaactttaagtagcagcctgtcttcttct7140

ctgcagtcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaatgcc7200

gcttctattatagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggttcaa7260

aatagtataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtcgat7320

caagcaatagcaaatgtagaggctaacgcagatggatcatcaggaacaggagttgcagga7380

caagaacaatctatttcacaatccatatatacttcaatttccactgctctttctcaactg7440

gcagcaccggctccagcacctgcaccgagacttgctcctagaccactaccagccccaatt7500

caagccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtc7560

gtttcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgccttcgca7620

cagtcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattctgcagca7680

tcaactgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgattat7740

tttaacacgcaaactttaagtagcagcctgtcttcttctcagcaatcactcagtgcactc7800

aattcgatagcgagtggtcaactgagctcctcgaatgccgcttctattttagcgagtgca7860

gtggcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacagttg7920

agaagcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtg7980

gctaacgcagttggagcatcaggaactggagttgcaggacaagaacaatctatttcacaa8040

tccatatatacttcaatttccactgctctttctcaactggcagcaccggctccagcacct8100

gcaccgagacttgctcctagaccactaccagccccaattcaagccccaagaccagcaccc8160

gcaccacaacctgcaccggtttacgcaccagccccagtcgtttcacaagttcaggcaact8220

tcttcttctcaagcctcggctcaacagagtgccttcgcacagtcccaacaatcttcagtt8280

gttcaatctcaacaaagttcaaacgcttattctgcagcatcaactgccggttcaagtgtg8340

tcgcaatctcaggcgattgtctcaagcgctcctgattattttaacacgcaaactttaagt8400

agcagcctgtcttcttctcagcaatcactcagtgcactcaattcgatagcgagtggtcaa8460

ctgagctcctcgaatgccgcttctattttagcgagtgcagtggcacggtcacttggagtg8520

tcccaagcgtcggttcaaaatagtataagccaacagttgagaagcgtagggcccggatct8580

tccacgtcctctgtcgctcaagcaatagcaaatggagtggctaacgcagttggagcatca8640

ggaactggagttgcaggacaagaacaatctatttcacaatccatatatacttcaatttcc8700

actgctctttctcaactggcagcaccggctccagcacctgcaccgagacttgctcctaga8760

ccactaccagccccaattcaagccccaagaccagcacccgcaccacaacctgcaccggtt8820

tacgcaccagccccagtcgtttcacaagttcaggcaacttcttcctctcaagcctcggct8880

caacagagtgccttcgcacagtcccaacaatcttcagttgttcaatctcaacaaagttca8940

aacgcttattctgcagcatcaactgccggttcaagtgtgtcgcaatctcaggcgattgtc9000

tcaagcgctcctgattattttaacacgcaaactttaagtagcagcctgtcttcttctcag9060

caatcactcagtgcactcaattcgatagcgagtggtcaactgagctcctcgaatgccgct9120

tctattttagcgagtgcagtggcacggtcacttggagtgtcccaagcgtcggttcaaaat9180

agtataagccaacagttgagaagcgtagggcccggatcttccacgtcctctgtcgctcaa9240

gcaatagcaaatggagtggctaacgcagttggagcatcaggaactggagttgcaggacaa9300

gaacaatctatttcacaatccatatatacttcaatttccactgctctttctcaactggca9360

gcaccggctccagcacctgcacctagaccagctcctcgaccactaccagccccaattcaa9420

gccccaagaccagcacccgcaccacaacctgcaccggtttacgcaccagccccagtcgtt9480

tcacaagttcaggcaacttcttcctctcaagcctcggctcaacagagtgccttcgcacag9540

tcccaacaatcttcagttgttcaatctcaacaaagttcaaacgcttattctgcagcatca9600

actgccggttcaagtgtgtcgcaatctcaggcgattgtctcaagcgctcctgtttatttt9660

aacacgcaaactttaagtagcagcctgtcttcttctctgcaatcactcagtgcactcaat9720

tcgatagcgagtggtcaactgagctcctcgaatgccgcttctattatagcgagtgcagtg9780

gcacggtcacttggagtgtcccaagcgtcggttcaaaatagtataagccaacagttgaga9840

agcgtagggcccggatcttccacgtcctctgtcgctcaagcaatagcaaatggagtggct9900

aacgcagttggagcatcaggaacaggagttgcaggacaagaacaatctatttcacaatcc9960

atatatacttcaatttccactgctctttctcaactggcagcaacggctccagcacctgca10020

ccgagaccagctcctcgaccactaccagccccaattcaagccccaagaccagcacccgca10080

ccacaacctgcgccagtttacgcacccgcgccagtg10116

<210>5

<211>327

<212>dna

<213>人工序列

<400>5

tacgctccagcgccagtgtacgctccagcgccaatcgtttcccagattcaggaaacttct60

tcttctcaagcctcggcgcaacagaattatttggcacagtcccagcaatcttccgttgct120

caatctcaacaaagttcgaacgcttattcggcagtatcagctgctgattcctcattttca180

agcggagcttcatcaggatttggctcttctctgtcttcttcctctggcttttctgcccca240

gccagccccgcgccgtattctccctcagtttccgcaagttcttcgattggagcctcgtcc300

ggatctgcgttggccgcttctgccgcc327

<210>6

<211>267

<212>dna

<213>人工序列

<400>6

cagcaactcatgtctcccgcagcgaaccaaaggattgcggctctgtccaattctctgagg60

gctgctgtatccggaggacaagtcaactatggcgccctctccaactcgcttgccagcgca120

gcgagtcagatacagagttcatccggcttgtccaagagtgaagttttagtcgaggctctc180

ctggaaacgttgtttgctctgctgagctctctcacgtctggagcgtctacggctgatttc240

gcacaggtcgtacttgcagcacttgga267

<210>7

<211>21

<212>dna

<213>人工序列

<400>7

atgttycaratgagttcaatg21

<210>8

<211>21

<212>dna

<213>人工序列

<400>8

ctgttgtgtttgagcggattg21

<210>9

<211>20

<212>dna

<213>人工序列

<400>9

tttcaatagctgccgcctgc20

<210>10

<211>22

<212>dna

<213>人工序列

<400>10

gtggcaatagcttgggttacgc22

<210>11

<211>27

<212>dna

<213>人工序列

<400>11

actcctgtggaaccatcggacgggggg27

<210>12

<211>33

<212>dna

<213>人工序列

<400>12

atgtcttggaccctggggcttcagtttttattc33

<210>13

<211>24

<212>dna

<213>人工序列

<400>13

ctatccaagtgctgcaagtacgac24

<210>14

<211>21

<212>dna

<213>人工序列

<400>14

tattctgcagcatcaactgcc21

<210>15

<211>24

<212>dna

<213>人工序列

<400>15

agcgtttgagctttgttgagattg24

<210>16

<211>3976

<212>prt

<213>人工序列

<400>16

metsertrpthrleuglyleuglnpheleuphethrphecysleuval

151015

leuphealatrpargglyaspalaleuseraspprolyssertrpval

202530

glnaspglualathralavaltyrpheleugluargthrleuglutyr

354045

leuglyglucysglyvalleulysileaspglngluargaspalaile

505560

aspthrmetpheglnmetsersermetpheglnglnasnalalyspro

65707580

serlysilelyshisserileserserlysleualaaspilevalile

859095

gluglyleugluglyaspseraspprosertyrlysileaspcysval

100105110

thrglnalailealathralapheglualathrthrglyvalargasp

115120125

gluasnpheilegluservalilegluleuilevalleuilepheasn

130135140

asnaspilegluglulysmetgluglugluileglugluthralaala

145150155160

serglnglnalaalaalailegluasnglnileserthrilegluser

165170175

asnpheglyseralaglnthrglnserservalglnasnaspilegln

180185190

glnglnserthrvalaspileserserservalthrasnglnilegln

195200205

serglnglntyralaaspserglnserseralaglnileglnsergln

210215220

glnservalalaserglnglnglnaspleuthrserglnleuthrthr

225230235240

serseralaglnglnglntyrgluthrserglnalaserilealagln

245250255

glnglntyrgluthrserglnalaserilealaglnglnglntyrglu

260265270

alaserglnglnglnalaserilegluglnglnglntyrglualaser

275280285

glnalaserilealaglnglnglntyrglualaserglnglnglnala

290295300

serilegluglnglnglntyrglualaserglnalaserilealagln

305310315320

glnglntyrglualaserglnglnglnalaserilealaglnglngln

325330335

tyrglnserserglnglnglnalaserileasnglnglnglntyrglu

340345350

alaserglnglnglnsersersersergluglnglntyrthrthrser

355360365

gluglnglnileglnglnsergluasnalatyrthraspglnserasn

370375380

ileglnglnseralaglnthrglnglntyrserthrserglnglnglu

385390395400

glngluserseralasertyrserthralaserilealaglyserser

405410415

valserglnserglnalathrvalserseralaproasptyrpheasn

420425430

thrglnthrleuserserserleuserserserleuglnserleuser

435440445

alaleuasnserilealaserglyglnleuserserserasnalaala

450455460

serileleualaseralavalalaglnserleuglyvalserglnala

465470475480

servalglnasnserileserglnglnleuargservalglyprogly

485490495

serserthrserservalalaglnalailealaasnglyvalalaasn

500505510

alavalglyalaserglythrglyvalalaglyglngluglnserile

515520525

serglnseriletyrthrserileserthralaleuserglnleuala

530535540

alaproalaproalaproalaproargleualaproargproleupro

545550555560

alaproileglnalaproargproalaproalaproglnproalapro

565570575

valtyralaproalaprovalvalserglnvalglnalathrserser

580585590

serglnalaseralaglnglnseralaphealaglnserglnglnser

595600605

servalvalglnserglnglnserserasnalatyrseralaalaser

610615620

thralaglyserservalserglnserglnalailevalserserala

625630635640

provaltyrpheasnthrglnthrleuserserserleuserserser

645650655

leuglnserleuseralaleuasnserleualaserglyglnleuser

660665670

sertrpasnalaalaserileilealaseralavalalaproserleu

675680685

glyvalserglnalaservalglnasnserileserglnglnleuarg

690695700

servalglyproglyserserthrserservalalaglnalaileala

705710715720

asnglyvalalaasnalavalglyalaserglythrglyvalalagly

725730735

glngluglnserileserglnseriletyrthrservalserthrala

740745750

leuserglnleualaalaproalaproalaproalaproargproala

755760765

proargproleuproalaproileglnalaproargproalaproala

770775780

proglnproalaprovaltyralaproalaprovalvalserglnval

785790795800

glnalathrserserserglnalaseralaglnglnseralapheala

805810815

glnserglnglnserservalvalglnserglnglnserserasnala

820825830

tyrseralaalaserservalglyserserpheserglnserglngly

835840845

thrvalproseralaprovaltyrpheasnthrglnthrleuserser

850855860

serleuserserserleuglnserleuseralaleuasnserileala

865870875880

serglyglnleuserserserasnalaalaserileilealaserala

885890895

valalaargserleuglyvalserglnalaservalglnasnserile

900905910

serglnglnleuargservalglyproglyserserthrserserval

915920925

alaglnalailealaasnglyvalalaasnalavalglyalasergly

930935940

thrglyvalalaglyglngluglnserileserglnseriletyrthr

945950955960

serileserthralaleuserglnleualaalaproalaproalapro

965970975

alaproargleualaproargproleuproalaproileglnalapro

980985990

argproalaproalaproglnproalaprovaltyralaproalapro

99510001005

valvalserglnvalglnalathrserserserglnalaserala

101010151020

glnglnseralaphealaglnserglnglnserservalalagln

102510301035

serglnglnserserasnvalaspseralaalaserservalgly

104010451050

serserpheserglnserglnglythrvalproseralaproval

105510601065

tyrpheasnthrglnthrleuserserserleuserserserleu

107010751080

glnserleuseralaleuasnserilealaserglyglnleuser

108510901095

sersertyralaaspserileleualaseralavalalaargser

110011051110

leuglyvalserglnalaservalleuasnserileserglngln

111511201125

leuargservalglyproglyserserthrserservalalagln

113011351140

alailealaasnglyvalalaasnalavalglyalaserglythr

114511501155

glyvalalaglyglngluglnserileserglnseriletyrthr

116011651170

servalserthralaleuserglnleualaalaproalaproala

117511801185

proalaproargleualaproargproleuproalaproilegln

119011951200

alaproargproalaproalaproglnproalaprovaltyrala

120512101215

proalaprovalvalserglnvalglnalathrsersersergln

122012251230

alaseralaglnglnseralaphealaglnserglnglnserser

123512401245

valvalglnserglnglnserserasnalatyrseralaalaser

125012551260

thralaglyserservalserglnserglnalailevalserser

126512701275

alaproasptyrpheasnthrglnthrleuserserserleuser

128012851290

serserglnglnserleuseralaleuasnserilealasergly

129513001305

glnleuserserserasnalaalaserileleualaseralaval

131013151320

alaargserleuvalvalserhisalaservalglnasnserile

132513301335

serglnglnleuargservalglyproglyserserthrserser

134013451350

valalaglnalailealaasnglyvalalaasnalavalglyala

135513601365

serglythrglyvalalaglyglngluglnserileserglnser

137013751380

iletyrthrserileserthralaleuserglnleualaalapro

138513901395

alaproalaproalaproargleualaproargproleuproala

140014051410

proileglnalaproargproalaproalaproglnproalapro

141514201425

valtyralaproalaprovalvalserglnvalglnalathrser

143014351440

serserglnalaseralaglnglnseralaphealaglnsergln

144514501455

glnserservalvalglnserglnglnserserasnalatyrser

146014651470

alaalaserthralaglyserservalserglnserglnalaile

147514801485

valserseralaproasptyrpheasnthrglnthrleuserser

149014951500

serleuserserserglnglnserleuseralaleuasnserile

150515101515

alaserglyglnleuserserserasnalaalaserileleuala

152015251530

seralavalalaargserleuglyvalserglnalaservalgln

153515401545

asnserileserglnglnleuargservalglyproglyserser

155015551560

thrserservalalaglnalailealaasnglyvalalaasnala

156515701575

valglyalaserglythrglyvalalaglyglngluglnserile

158015851590

serglnseriletyrthrserileserthralaleuserglnleu

159516001605

alaalaproalaproalaproalaproargleualaproargpro

161016151620

leuproalaproileglnalaproargproalaproalaprogln

162516301635

proalaprovaltyralaproalaprovalvalserglnvalgln

164016451650

alathrserserserglnalaseralaglnglnseralapheala

165516601665

glnserglnglnserservalvalglnserglnglnserserasn

167016751680

alatyrseralaalaserthralaglyserservalserglnser

168516901695

glnalailevalserseralaproasptyrpheasnthrglnthr

170017051710

leuserserserleuserserserglnglnserleuseralaleu

171517201725

asnserilealaserglyglnleuserserserasnalaalaser

173017351740

ileleualaseralavalalaargserleuglyvalserglnala

174517501755

servalglnasnserileserglnglnleuargservalglypro

176017651770

glyserserthrserservalalaglnalailealaasnglyval

177517801785

alaasnalavalglyalaserglythrglyvalalaglyglnglu

179017951800

glnserileserglnseriletyrthrserileserthralaleu

180518101815

serglnleualaalaproalaproalaproalaproargleuala

182018251830

proargproleuproalaproileglnalaproargproalapro

183518401845

alaproglnproalaprovaltyralaproalaprovalvalser

185018551860

glnvalglnalathrserserserglnalaseralaglnglnser

186518701875

alaphealaglnserglnglnserservalvalglnserglngln

188018851890

serserasnalatyrseralaalaserthralaglyserserval

189519001905

serglnserglnalailevalserseralaproasptyrpheasn

191019151920

thrglnthrleuserserserleuserserserglnglnserleu

192519301935

seralaleuasnserilealaserglyglnleuserserserasn

194019451950

alaalaserileleualaseralavalalaargserleuglyval

195519601965

serglnalaservalglnasnserileserglnglnleuargser

197019751980

valglyproglyserserthrserservalalaglnalaileala

198519901995

asnglyvalalaasnalavalglyalaserglythrglyvalala

200020052010

glyglngluglnserileserglnseriletyrthrserileser

201520202025

thralaleuserglnleualaalaproalaproalaproalapro

203020352040

argleualaproargproleuproalaproileglnalaproarg

204520502055

proalaproalaproglnproalaprovaltyralaproalapro

206020652070

valvalserglnvalglnalathrserserserglnalaserala

207520802085

glnglnseralaphealaglnserglnglnserservalvalgln

209020952100

serglnglnserserasnalatyrseralaalaserthralagly

210521102115

serservalserglnserglnalailevalserseralaproasp

212021252130

tyrpheasnthrglnthrleuserserserleusersersergln

213521402145

glnserleuseralaleuasnserilealaserglyglnleuser

215021552160

serserasnalaalaserileleualaseralavalalaargser

216521702175

leuglyvalserglnalaservalglnasnserileserglngln

218021852190

leuargservalglyproglyserserthrserservalalagln

219522002205

alailealaasnglyvalalaasnalavalglyalaserglythr

221022152220

glyvalalaglyglngluglnserileserglnseriletyrthr

222522302235

serileserthralaleuserglnleualaalaproalaproala

224022452250

proalaproargleualaproargproleuproalaproilegln

225522602265

alaproargproalaproalaproglnproalaprovaltyrala

227022752280

proalaprovalvalserglnvalglnalathrsersersergln

228522902295

alaseralaglnglnseralaphealaglnserglnglnserser

230023052310

valvalglnserglnglnserserasnalatyrseralaalaser

231523202325

thralaglyserservalserglnserglnalailevalserser

233023352340

alaproasptyrpheasnthrglnthrleuserserserleuser

234523502355

serserglnglnserleuseralaleuasnserilealasergly

236023652370

glnleuserserserasnalaalaserileleualaseralaval

237523802385

alaargserleuglyvalserglnalaservalglnasnserile

239023952400

serglnglnleuargservalglyproglyserserthrserser

240524102415

valalaglnalailealaasnglyvalalaasnalavalglyala

242024252430

serglythrglyvalalaglyglngluglnserileserglnser

243524402445

ileaspthrserileserthralaleuserglnleualaalapro

245024552460

alaproalaproalaproargleualaproargproleuproala

246524702475

proileglnalaproargproalaproalaproglnproalapro

248024852490

valtyralaproalaprovalvalserglnvalglnalathrser

249525002505

serserglnalaseralaglnglnseralaphealaglnsergln

251025152520

glnserservalvalglnserglnglnserserasnalatyrser

252525302535

alaalaserthralaglyserservalserglnserglnalaile

254025452550

valserseralaproasptyrpheasnthrglnthrleuserser

255525602565

serleuserserserglnglnserleuseralaleuasnserile

257025752580

alaserglyglnleuserserserasnalaalaserileleuala

258525902595

seralavalalaargserleuglyvalserglnalaservalgln

260026052610

asnserileserglnglnleuargservalglyproglyserser

261526202625

thrserservalalaglnalailealaasnglyvalalaasnala

263026352640

valglyalaserglythrglyvalalaglyglngluglnserile

264526502655

serglnseriletyrthrserileserthralaleuserglnleu

266026652670

alaalaproalaproalaproalaproargleualaproargpro

267526802685

leuproalaproileglnalaproargproalaproalaprogln

269026952700

proalaprovaltyralaproalaprovalvalserglnvalgln

270527102715

alathrserserserglnalaseralaglnglnseralapheala

272027252730

glnserglnglnserservalvalglnserglnglnserserasn

273527402745

alatyrseralaalaserthralaglyserservalserglnser

275027552760

glnalailevalserseralaprovaltyrpheasnthrglnthr

276527702775

leuserserserleuserserserleuglnserleuseralaleu

278027852790

asnserilealaserglyglnleuserserserasnalaalaser

279528002805

ileilealaseralavalalaargserleuglyvalserglnala

281028152820

servalglnasnserileserglnglnleuargservalglypro

282528302835

glyserserthrserservalaspglnalailealaasnvalglu

284028452850

alaasnalaaspglyserserglythrglyvalalaglyglnglu

285528602865

glnserileserglnseriletyrthrserileserthralaleu

287028752880

serglnleualaalaproalaproalaproalaproargleuala

288528902895

proargproleuproalaproileglnalaproargproalapro

290029052910

alaproglnproalaprovaltyralaproalaprovalvalser

291529202925

glnvalglnalathrserserserglnalaseralaglnglnser

293029352940

alaphealaglnserglnglnserservalvalglnserglngln

294529502955

serserasnalatyrseralaalaserthralaglyserserval

296029652970

serglnserglnalailevalserseralaproasptyrpheasn

297529802985

thrglnthrleuserserserleuserserserglnglnserleu

299029953000

seralaleuasnserilealaserglyglnleuserserserasn

300530103015

alaalaserileleualaseralavalalaargserleuglyval

302030253030

serglnalaservalglnasnserileserglnglnleuargser

303530403045

valglyproglyserserthrserservalalaglnalaileala

305030553060

asnglyvalalaasnalavalglyalaserglythrglyvalala

306530703075

glyglngluglnserileserglnseriletyrthrserileser

308030853090

thralaleuserglnleualaalaproalaproalaproalapro

309531003105

argleualaproargproleuproalaproileglnalaproarg

311031153120

proalaproalaproglnproalaprovaltyralaproalapro

312531303135

valvalserglnvalglnalathrserserserglnalaserala

314031453150

glnglnseralaphealaglnserglnglnserservalvalgln

315531603165

serglnglnserserasnalatyrseralaalaserthralagly

317031753180

serservalserglnserglnalailevalserseralaproasp

318531903195

tyrpheasnthrglnthrleuserserserleusersersergln

320032053210

glnserleuseralaleuasnserilealaserglyglnleuser

321532203225

serserasnalaalaserileleualaseralavalalaargser

323032353240

leuglyvalserglnalaservalglnasnserileserglngln

324532503255

leuargservalglyproglyserserthrserservalalagln

326032653270

alailealaasnglyvalalaasnalavalglyalaserglythr

327532803285

glyvalalaglyglngluglnserileserglnseriletyrthr

329032953300

serileserthralaleuserglnleualaalaproalaproala

330533103315

proalaproargleualaproargproleuproalaproilegln

332033253330

alaproargproalaproalaproglnproalaprovaltyrala

333533403345

proalaprovalvalserglnvalglnalathrsersersergln

335033553360

alaseralaglnglnseralaphealaglnserglnglnserser

336533703375

valvalglnserglnglnserserasnalatyrseralaalaser

338033853390

thralaglyserservalserglnserglnalailevalserser

339534003405

alaproasptyrpheasnthrglnthrleuserserserleuser

341034153420

serserglnglnserleuseralaleuasnserilealasergly

342534303435

glnleuserserserasnalaalaserileleualaseralaval

344034453450

alaargserleuglyvalserglnalaservalglnasnserile

345534603465

serglnglnleuargservalglyproglyserserthrserser

347034753480

valalaglnalailealaasnglyvalalaasnalavalglyala

348534903495

serglythrglyvalalaglyglngluglnserileserglnser

350035053510

iletyrthrserileserthralaleuserglnleualaalapro

351535203525

alaproalaproalaproargproalaproargproleuproala

353035353540

proileglnalaproargproalaproalaproglnproalapro

354535503555

valtyralaproalaprovalvalserglnvalglnalathrser

356035653570

serserglnalaseralaglnglnseralaphealaglnsergln

357535803585

glnserservalvalglnserglnglnserserasnalatyrser

359035953600

alaalaserthralaglyserservalserglnserglnalaile

360536103615

valserseralaprovaltyrpheasnthrglnthrleuserser

362036253630

serleuserserserleuglnserleuseralaleuasnserile

363536403645

alaserglyglnleuserserserasnalaalaserileileala

365036553660

seralavalalaargserleuglyvalserglnalaservalgln

366536703675

asnserileserglnglnleuargservalglyproglyserser

368036853690

thrserservalalaglnalailealaasnglyvalalaasnala

369537003705

valglyalaserglythrglyvalalaglyglngluglnserile

371037153720

serglnseriletyrthrserileserthralaleuserglnleu

372537303735

alaalathralaproalaproalaproargproalaproargpro

374037453750

leuproalaproileglnalaproargproalaproalaprogln

375537603765

proalaprovaltyralaproalaprovaltyralaproalapro

377037753780

valtyralaproalaproilevalserglnileglngluthrser

378537903795

serserglnalaseralaglnglnasntyrleualaglnsergln

380038053810

glnserservalalaglnserglnglnserserasnalatyrser

381538203825

alavalseralaalaaspserserpheserserglyalaserser

383038353840

glypheglyserserleuserserserserglypheseralapro

384538503855

alaserproalaprotyrserproservalseralaserserser

386038653870

ileglyalaserserglyseralaleualaalaseralaalagln

387538803885

glnleumetserproalaalaasnglnargilealaalaleuser

389038953900

asnserleuargalaalavalserglyglyglnvalasntyrgly

390539103915

alaleuserasnserleualaseralaalaserglnileglnser

392039253930

serserglyleuserlyssergluvalleuvalglualaleuleu

393539403945

gluthrleuphealaleuleuserserleuthrserglyalaser

395039553960

thralaaspphealaglnvalvalleualaalaleugly

396539703975