纤维素是由β-1,4键连接的葡萄糖分子组成的聚合物，其对分解或解聚合非常有耐性。一旦纤维素转化为葡萄糖，后者容易用酵母发酵成生物燃料，如乙醇。研究的用于将纤维素转化为葡萄糖的最古老的方法是基于酸水解。该工艺可以在浓酸或稀酸的存在下进行。然而，几个缺点(例如当使用浓酸时酸回收率很差，以及使用稀酸的情况下葡萄糖的产量低)对于酸水解工艺的经济性是不利的。为了克服酸水解工艺的缺点，最近的纤维素转化工艺已经更多地与使用纤维素酶类型的酶的酶促水解相关。然而，该木质纤维素生物质(如纤维素)的酶促水解的缺点是其工业生产工艺较为昂贵。因此，有必要使用日益有效的分泌纤维素酶的微生物菌株。在这方面，很多微生物包含水解纤维素的酶，例如真菌-木霉属(Trichoderma)、曲霉属(Aspergilus)、腐质霉属(Humicola)、或镰刀菌属(Fusarium)，以及细菌例如高温单孢菌属(Thermomonospora)、芽孢杆菌属(Bacilus)、纤维单胞菌属(Celulomonas)和链霉菌属(Streptomyces)。由这些微生物分泌的酶具有用于将纤维素转化为葡萄糖的三种不同类型的活性，可以分为三组：随机攻击纤维素纤维内部的内切葡聚糖酶，攻击纤维末端释放纤维二糖的外切葡聚糖酶，和将该纤维二糖水解为葡萄糖的β-葡萄糖苷酶。其他类型的酶，例如半纤维素酶或最近发现的一类多糖单加氧酶也可以在水解功效中起作用。工业上强烈需要降低酶促水解的成本，而这种将低涉及使用降低的酶剂量，从而使用更有效的酶鸡尾酒。因此，一些专利申请描述了活性高于里氏木霉(Trichodermareesei)的天然酶或通过基因工程改进的变体。可以提及的有涉及外切葡聚糖酶的专利申请US2010304464、WO2010/066411和WO2013/029176，涉及内切葡聚糖酶的申请WO2007/109441、WO2012/149192和WO2010/076388，涉及β-葡萄糖苷酶的申请WO2010/029259、WO2010/135836或WO2010/022518，或涉及多糖单加氧酶的申请WO12135659和WO12149344。主要基于结构标准，水解木质纤维素生物质的酶在CAZy系统中分类(Cantarel,B.L.,Coutinho,P.M.,Rancurel,C.,Bernard,T.,Lombard,V.,&Henrissat,B.(2009).TheCarbohydrate-ActiveEnZymesdatabase(CAZy):anexpertresourceforGlycogenomics.Nucleicacidsresearch,37,D233-8)。外切葡聚糖酶可属于GH6、7、9、48和74家族。为了使木质纤维素生物质水解有效且具有经济效益，酶混合物必须包含平衡比例的具有各种酶活性的酶，特别包括但不排除外切葡聚糖酶、内切葡聚糖酶、木聚糖酶和β-葡萄糖苷酶类型。举例而言，在里氏木霉的天然混合物中，一般注意到存在60-70％的外切葡聚糖酶、15-20％的内切葡聚糖酶、几个百分点的半纤维素酶和约的5-10％的β-葡萄糖苷酶。该混合物适于以可接受的收率水解大部分经预处理的物质(例如在酸条件下汽爆的小麦秸秆类型)。在混合物中已经存在相当比例的外切葡聚糖酶表明，增加这些酶的量而不损害其他活性将非常困难。里氏木霉的基因组包含两种外切葡聚糖酶，一种来自家族6(CBH2,cel6a)，另一种来自家族7(CBH1,Cel7a)。它们分别将纤维素的非还原(EC3.2.1.176)和还原(EC3.2.1.91)末端水解为纤维二糖。水解和发酵可以根据各种方案进行。最常见的由独立的水解和发酵(SHF)组成。该方法能够通过维持最佳反应条件来优化各个步骤。该发酵在约28℃至约30℃的温度即时进行，而水解一般在至少45℃的温度进行。然而，在SHF中，在反应结束时释放的糖浓度非常高，导致对酶的抑制，降低了该方法的效率。为了避免这些缺点，可以设想另一种方法。在SSF中，两个步骤(己糖的水解和发酵)同时发生，防止糖积聚到抑制酶的浓度。由于使用单一反应器，投资成本也降低了。由于没有抑制，水解率更高，因为释放的糖立即被用于发酵成乙醇。在该方法中，反应器的温度必然在水解和发酵的最佳温度之间形成平衡，一般在约30℃至约35℃。然而，纤维素分解酶的活性在该温度降低约30％。SSF也允许分解纤维素的酶在发酵糖的生物中表达，从而能够限制，或在极端情况下消除在单独步骤产生的酶的来源。因此，获得在水解和发酵的最佳温度(即，30℃-50℃)下保留有效的外切葡聚糖酶活性的酶，同时保持混合物中所有酶的比例，对将木质纤维素生物质转化为生物燃料的工艺而言是显著的增益。本发明人已经开发了具有增强的外切葡聚糖酶活性的多肽，尤其是与序列SEQIDNO：2的CBH2参考蛋白的外切葡聚糖酶活性相比。CBH2对应于来自里氏木霉的外切葡聚糖酶2。在这方面，值得赞扬的是，申请人在大量研究后已经分离或纯化与CBH2参考蛋白(SEQIDNO：2)的外切葡聚糖酶活性相比，外切葡聚糖酶活性增强的多肽。因此，本发明涉及选自以下的多肽：i.选自SEQIDNO:4、SEQIDNO:6、SEQIDNO:8、SEQIDNO:10、SEQIDNO:12、SEQIDNO:14、SEQIDNO:16、SEQIDNO:18、SEQIDNO:20、SEQIDNO:22、SEQIDNO:24、SEQIDNO:26和SEQIDNO:28的氨基酸序列；和ii.与序列SEQIDNO:6、SEQIDNO:8、SEQIDNO:10、SEQIDNO:12、SEQIDNO:14、SEQIDNO:16、SEQIDNO:18、SEQIDNO:20、SEQIDNO:22、SEQIDNO:24、SEQIDNO:26和SEQIDNO:28相比具有至少70％，优选至少75％、80％、85％、90％、95％、98％或99％百分比的相同残基(同一性百分比)的氨基酸序列。优选的，上述多肽的特征在于，其在发酵生物中的表达至少与CBH2参考蛋白(SEQIDNO：2)的表达相等。根据本发明，给定序列相对于SEQIDNO:4、6、8、10、12、14、16、18、20、22、24、26或28的同一性百分比对应于该给定序列与SEQIDNO:4、6、8、10、12、14、16、18、20、22、24、26或28之间相同的残基数除以SEQIDNO:4、6、8、10、12、14、16、18、20、22、24、26或28的残基数。在一个优选的实施方案中，在约35℃和/或约50℃的温度下，与氨基酸序列SEQIDNO：2的CBH2多肽的外切葡聚糖酶活性相比，本发明的多肽的外切葡聚糖酶活性增强至少10％，优选至少20％，优选至少30％，甚至更优选至少40％。例如，本领域技术人员将能够使用底物例如纤维素纤维素PASC或使用显色底物(对硝基苯基糖苷)例如pNP乳糖苷来测定酶活性的增加，或换言之增强。将分别通过释放的还原糖或硝基苯酚的比色法分析来显示酶活性。本领域技术人员能够用来测定根据本发明的多肽与CBH2参考蛋白(SEQIDNO：2)相比，酶活性是否增强的实验方案的一个实例如下：–制备表达根据本发明的重组酶的解脂耶罗维亚酵母(Y.lipolytica)的原种培养物，于28℃过夜；–用一定体积的原种培养物接种表达培养基使其在培养开始时在600nm的光密度为0.2；–于28℃培养所述细胞96小时；–在8000rpm离心5分钟；–于35℃和50℃用100μl含有1％还原纤维糊精(CD)的pH6的0.1M柠檬酸磷酸缓冲液孵育100μl上清液4小时；–取出100μl反应液；–加入100μlDNS试剂；–于100℃孵育5分钟；–在冰上孵育3分钟；–在3000rpm离心10分钟；–用150μl在540nm读取OD。本发明的一个主题还是编码至少一种如上所述多肽的纯化或分离的核酸。下表1包含来自里氏木霉的参考基因CBH2、来自鞭毛藻丛赤壳菌(Nectriahaematococca，NH)和来自小麦赤霉菌(Giberelazeae，GZ)的推定的外切葡聚糖酶的核酸和肽序列，以及本发明的多肽和核酸的鉴定。表1克隆核酸多肽CBH2(野生型)SEQIDNO:1SEQIDNO:235B7SEQIDNO:3SEQIDNO:495B7SEQIDNO:5SEQIDNO:6100F11SEQIDNO:7SEQIDNO:8139F12SEQIDNO:9SEQIDNO:10157B11SEQIDNO:11SEQIDNO:12161A1SEQIDNO:13SEQIDNO:14161C12SEQIDNO:15SEQIDNO:16189H8SEQIDNO:17SEQIDNO:18196D9SEQIDNO:19SEQIDNO:20198E11SEQIDNO:21SEQIDNO:22251B4SEQIDNO:23SEQIDNO:24251C4SEQIDNO:25SEQIDNO:26382A2SEQIDNO:27SEQIDNO:28GZ基因SEQIDNO:29SEQIDNO:30NH-7基因SEQIDNO:31SEQIDNO:32本发明的一个主题还是编码至少一种如上所述多肽的纯化或分离的核酸。优选的，所述纯化或分离的核酸选自以下序列：SEQIDNO:3、SEQIDNO:5、SEQIDNO:7、SEQIDNO:9、SEQIDNO:11、SEQIDNO:13、SEQIDNO:15、SEQIDNO:17、SEQIDNO:19、SEQIDNO:21、SEQIDNO:23、SEQIDNO:25和SEQIDNO:27。根据本发明，如上所述的核酸可以与启动子、终止子或其在宿主细胞中的表达所需的任何其他序列功能性连接。本发明还涉及包含至少一个如上所述的核酸的载体。根据本发明，术语“载体”意欲是指可能在其中插入外源核酸片段的任何DNA序列，载体能够将外源DNA引入宿主细胞。作为载体，可以提及(不排除其他)：质粒、黏粒、酵母人工染色体(YAC)、细菌人工染色体(BAC)、衍生自P1噬菌体的人工染色体(PAC)或来自病毒的载体。根据本发明的载体还可以携带可选择标志物。术语“可选择标志物”意欲是指其表达赋予包含它的细胞使得能够选择它们这一特征的基因。例如抗生素抗性基因。本发明的一个主题还是包含至少一种如上所述的多肽、或至少一种如上所述的核酸、或至少一种如上所述的载体的分离的宿主细胞。本领域技术人员将能够通过熟知的常规方法将如上所述的多肽之一、核酸之一或载体之一引入宿主细胞。例如，可以提及氯化钙处理、电穿孔或使用基因枪。根据一个实施方案，本领域技术人员将能够通过常规方法将编码根据本发明的具有增强的外切葡聚糖酶活性的多肽的核酸的几个拷贝引入宿主细胞。根据一个实施方案，如上所述的分离的宿主细胞选自：木霉属(Trichoderma)、曲霉属(Aspergilus)、脉胞菌属(Neurospora)、腐质霉属(Humicola)、毁丝霉属(Myceliophthora)、金孢子菌属(Chrysosporium)、青霉属(Penicilium)、镰刀菌属(Fusarium)、高温单孢菌属(Thermomonospora)、芽孢杆菌属(Bacilus)、假单包菌属(Pesudomonas),埃希氏菌属(Escherichia)、梭状芽孢杆菌(Clostridium)、纤维单胞菌属(Celulomonas)、链霉菌属(Streptomyces)、耶罗维亚酵母属(Yarrowia)、毕赤酵母属(Pichia)和酵母属(Saccharomyces)。根据一个优选的实施方案，如上所述的宿主细胞选自：里氏木霉、Trichodermaviridae、康氏木霉(Trichodermakoningi)、黑曲霉(Aspergilusniger)、构巢曲霉(Aspergilusnidulans)、文氏曲霉(Aspergiluswenti)、米曲霉(Aspergilusoryzae)、海枣曲霉(Aspergilusphoenicis)、嗜热毁丝霉(Myceliophthorathermopila)、Chrysosporiumlucknowense、粗糙脉孢菌(Neurosporacrassa)、灰腐质霉(Humicolagrisae)、嗜松青霉(Peniciliumpinophilum)、草酸青霉(Peniciliumoxalicum)、大肠杆菌(Escherichiacoli)、丙酮丁醇梭菌(Clostridiumacetobutylicum)、糖解梭菌(Clostridiumsaccharolyticum)、拜氏梭菌(Clostridiumbenjerincki)、丁酸梭菌(Clostridiumbutylicum)、毕赤酵母(Pichiapastoris)、解脂耶罗维亚酵母(Yarrowialipolityca)和酿酒酵母(Saccharomycescerevisiae)。根据一个优选的实施方案，如上所述的分离的宿主细胞选自里氏木霉和酿酒酵母。本发明的一个主题还是如上所述的任何一种多肽用于纤维素水解的用途。本发明的一个主题还是如上所述的任何一种多肽用于生产生物燃料的用途。根据本发明，术语“生物燃料”可以定义为来自生物质转化且可以用于能量目的的任何产物。首先，不希望被限制于此，可以提及的实例有生物气、可引入燃料(任选地在随后转化之后)或其本身可以作为燃料的产物，例如醇(乙醇、丁醇和/或异丙醇，取决于所用发酵生物的类型)、溶剂(丙酮)、酸(丁酸)、脂质及其衍生物(短链或长链脂肪酸、脂肪酸酯)和氢。优选的，根据本发明的生物燃料是醇，例如乙醇、丁醇和/或异丙醇。更优选的，根据本发明的生物燃料是乙醇。在另一个实施方案中，生物燃料是生物气。在另一个实施方案中，产物是在化学工业中具有优势的分子，例如另一种醇，诸如1,2-丙二醇、1,3-丙二醇、1,4-丙二醇或2,3-丁二醇，有机酸例如乙酸、丙酸、丙烯酸、丁酸、琥珀酸、苹果酸、延胡索酸、柠檬酸或衣康酸，或羟基酸例如乙醇酸，羟基丙酸和乳酸。生产用于木质纤维素水解的酶鸡尾酒的一个实施方案描述如下。在碳基底物例如乳糖或葡萄糖(选择用于微生物生长)存在下，在发酵器中培养能够表达至少一种根据本发明的多肽的丝状真菌(优选木霉属，更优选里氏木霉)的菌株。在一个实施方案中，取决于其性质，所述碳基底物在灭菌之前引入发酵器，或单独灭菌并在发酵器灭菌后引入发酵器，从而获得20-35g/l的初始浓度。然后加入含有选择用于酶生产的底物的水性溶液。最后通过将培养基离心回收真菌产生的作用于木质纤维素生物质的酶组合物。该组合物特别包含根据本发明的β-葡萄糖苷酶、内切葡聚糖酶和外切葡聚糖酶。在一个实施方案中，含有选择用于生产酶的底物的水性溶液以200-250g/l的浓度制备。该溶液优选还包含诱导底物，例如乳糖。在初始碳基底物耗尽后注入该水性溶液，以提供35-45mg/g细胞的最佳量(分批进料)。在该分批进料阶段期间，培养基中糖的残留浓度小于1g/l，通过真菌分泌作用于木质纤维素生物质的酶。所述酶可以通过离心培养基来回收。本发明的一个主题是能够作用于木质纤维素生物质的酶组合物，所述酶组合物由丝状真菌产生，且包含至少一种与CBH2参考蛋白的外切葡聚糖酶活性相比，外切葡聚糖酶活性增强的多肽。术语“丝状真菌”特别意欲是指木霉属，更优选里氏木霉。最后，本发明的一个主题是用于从生物质生产生物燃料的方法，包含以下连续步骤：–将待水解的生物质悬浮于水相中；–在如上所述的酶组合物存在下水解木质纤维素生物质，以产生含有葡萄糖的水解物；–在发酵生物存在下发酵水解物的葡萄糖，以产生发酵液；–将生物燃料与发酵液分离。在一个实施方案中，待水解的生物质以6％-40％，优选20％-30％的固体量悬浮于水相。将pH调节至4-5.5，优选4.8-5.2，温度为40-60℃，优选45-50℃。通过加入作用于木质纤维素生物质的酶组合物启动水解反应，通常使用的量是10-30mg分泌蛋白/g预处理底物，或更少。反应通常持续15-48小时。反应之后分析释放的糖，尤其是葡萄糖。通过过滤或离心然后在发酵单元中处理将糖溶液与未水解的固体部分(主要由木质素组成)分离。在发酵步骤后，例如通过蒸馏将生物燃料与发酵液分离。本发明的另一个主题是用于从生物质生产生物燃料的方法，其特征在于，它包含以下连续步骤：–将待水解的生物质悬浮于水相中；–向悬浮液同时加入如上所述的酶组合物和发酵生物，发酵混合物以产生发酵液；–将生物燃料与发酵液分离。优选的，同时加入酶组合物和发酵生物，然后在30-50℃的温度下孵育以产生发酵液。根据该实施方案，根据本领域技术人员已知的SSF(同时发生的糖化和发酵)方法，将存在于生物质中的纤维素转化为葡萄糖，同时，在同一反应器中，发酵生物(例如酵母)将葡萄糖转化为最终产物。取决于发酵生物的代谢和水解能力，可能需要加入或多或少显著量的外源细胞裂解混合物以使操作正确进行。在另一个实施方案中，发酵生物通过分泌或在其细胞表面产生本发明主题的多肽，任选和作用于木质纤维素生物质的其他酶一起，从而限制或消除对由丝状真菌产生的酶的需要。优选的，发酵生物是如上所述的宿主细胞。优选的，加入具有酶组合物的宿主细胞和/或发酵生物，然后在30-50℃的温度下孵育以产生发酵液。因此，使用根据本发明的具有更好外切葡聚糖酶活性的多肽具有以下优势：获得更好的葡萄糖生产收率，同时使用比之前更少的酶，从而还具有经济上的优势。本发明的其他方面、主题、优势和特征将通过实施例和附图的方式阅读以下描述本发明优选实施方案的非限制性描述展示。图1是显示用于筛选的DP3-DP11纤维糊精的MALDI-TOF质谱图。具体实施方式实施例1：制备还原纤维糊精(DP3-11)1-纤维素水解(改编自Y-H.PercivalZhang,L.R.LyndAnalyticalBiochemistry322(2003),225-232.)向160ml冷却至0℃的盐酸溶液分批加入20g纤维素(Avicel,CASNumber9004-34-6,Sigma-AldrichSaint-QuentinFallavier)，并剧烈搅拌。分几个步骤(4×10ml)向该溶液中加入预先冷却的硫酸。将该反应于24℃保持搅拌4小时，然后倒入冷却至-20℃的0.8l丙酮。搅拌2小时后，过滤出沉淀物，取400ml冷却的丙酮，然后再次过滤。然后将固体置于600ml水中，搅拌过夜以溶解纤维糊精(CD)。过滤出固体后，用300gAmberliteIRA400OH-树脂中和含有纤维糊精的可溶部分，然后冻干。然后在过滤前，在超声存在下，将冻干物重悬于500ml甲醇中30分钟以溶解低分子量的糖，然后再次冻干以获得6.8gDP3-11纤维糊精。对于筛选，选择具有最好可能分子量的底物进行，以尽可能模拟纤维素的结构。然而，高分子量的纤维糊精是不可溶的，这使该测试不容易重复。因此，选择DP5-7的纤维糊精，这表示所需的高分子量和仙湖微晶的溶解度之间的良好平衡。图1表示根据如上所述的方法一般获得的MALDI-TOF质谱。图1表明，分离的寡糖主要是DP5-7。2-纤维糊精的还原在稀释于120ml水的2gDP3-11纤维糊精中加入400mg硼氢化钠。在室温下搅拌3小时后，加入AmberliteH+IR120树脂中和溶液，过滤，然后冻干，以获得2g定量还原的纤维糊精(C.Schou,G.Rasmussen,M-B.Kaltoft,B.Henrissat,M.SchuleinEur.J.Biochem.217,947-953(1993))。用BCA(二喹啉甲酸)分析分离的纤维糊精能够确认末端的完全还原(Y.-H.PercivalZhang,L.R.LyndBiomacromolecules2005,6,1510-1515)。实施例2：通过L-洗牌(shuffling)的进化将里氏木霉的纤维二糖水解酶2基因(SEQIDNO：1)用与亲本基因CBH2(SEQIDNO：1)分别具有63％和69％同源性的来自小麦赤霉菌PH-1的推测的外切葡聚糖酶基因(SEQIDNO：29)和来自鞭毛藻丛赤壳菌mpVI的假想蛋白NECHADRAFT_73991基因(SEQIDNO：31)根据专利EP1104457中所述的专利方法进行一轮L-洗牌。在克隆中删除编码信号肽的核酸序列(SEQIDNO:33)，并用酵母的序列SEQIDNO：34(对应的信号肽序列：SEQIDNO:35)替换。1-高通量筛选开发高通量筛选试验以选择由洗牌产生的最佳克隆，即，与CBH2参考酶(SEQIDNO：2)相比，显示纤维二糖水解酶活性增强至少20％的那些。高通量筛选试验实验根据以下步骤进行：–在琼脂上分离表达根据本发明的酶的L-洗牌变体的解脂耶罗维亚酵母的克隆，并于28℃在YNBcasa培养基(酵母氮基1.7g/l、NH4Cl10g/l、葡萄糖10g/l、酪蛋白氨基酸2g/l,pH7)中预培养所述克隆36小时；–用所述预培养接种补充有12.5μg/ml的四环素的5％YTD培养基(酵母提取物10g/l、胰蛋白胨20g/l、葡萄糖2.5g/l,pH6.8)，然后于28℃孵育20小时；–用之前的培养物接种含有20g/l量的诱导剂(油酸)的10％表达培养基，然后于28℃孵育96小时-在1500rpm离心5分钟；–取出100μl上清液；–加入100μl在0.1M柠檬酸磷酸缓冲液(pH6)中的1g/l的还原CD，–于35℃孵育24小时；–在2500rpm离心5分钟；–取出80μl上清液；–加入80μlDNS试剂；–于105℃孵育12分钟，然后在冰上孵育5分钟；–用120μl在540nm读取光学密度(OD)。在这些筛选条件下，发现一些克隆中的纤维二糖水解酶活性与CBH2参考酶(SEQIDNO:2)相比增强(540nm的OD增加)。在这些克隆中，可以提及35B7、95B7、100F11、139F12、157B11、161A1、161C12、189H8、196D9、198E11、251B4、251C4和382A2，其分别编码酶SEQIDNO:4、SEQIDNO:6、SEQIDNO:8、SEQIDNO:10、SEQIDNO:12、SEQIDNO:14、SEQIDNO:16、SEQIDNO:18、SEQIDNO:20、SEQIDNO:22、SEQIDNO:24、SEQIDNO:26和SEQIDNO:28。2-测定纤维二糖水解酶活性的增强2-1、在还原纤维糊精底物上为了评估在第一轮洗牌中选择的变体相对于CBH2参考酶(SEQIDNO:2)的Kcat，进行以下过程：–制备表达根据本发明的重组酶的解脂耶罗维亚酵母的原种培养物，于28℃过夜；–用一定体积的原种培养物接种表达培养基使其在培养开始时在600nm的光密度为0.2；–于28℃培养所述细胞96小时；–在8000rpm离心5分钟；–于35℃和50℃用100μl含有1％还原纤维糊精(CD)的pH6的0.1M柠檬酸磷酸缓冲液孵育100μl上清液4小时；–取出100μl反应液；–加入100μlDNS试剂；–于100℃孵育5分钟；–在冰上孵育3分钟；–在3000rpm离心10分钟；–用150μl在540nm读取光密度。根据本发明，根据以下方法计算Kcat：–将测试中在540nm的OD作为培养物上清液的体积的函数作曲线图；–减去阴性对照的值；–除以葡萄糖标准率系数(用DNS显示的葡萄糖的各种量)；–除以反应时间(240分钟)。表2给出了在这些实验条件下，与CBH2参考蛋白(SEQIDNO：2)相比，分别编码酶SEQIDNO:4、SEQIDNO:6、SEQIDNO:8、SEQIDNO:10、SEQIDNO:12、SEQIDNO:14、SEQIDNO:16、SEQIDNO:18、SEQIDNO:20、SEQIDNO:22、SEQIDNO:24、SEQIDNO:26和SEQIDNO:28的35B7、95B7、100F11、139F12、157B11、161A1、161C12、189H8、196D9、198E11、251B4、251C4和382A2克隆所得的Kcat值和增强倍数。表2：在还原CD上纤维二糖水解酶活性的增强结果表明，无论在35℃或在50℃，与CBH2参考蛋白(SEQIDNO：2)相比，分别编码酶SEQIDNO:4、SEQIDNO:6、SEQIDNO:8、SEQIDNO:10、SEQIDNO:12、SEQIDNO:14、SEQIDNO:16、SEQIDNO:18、SEQIDNO:20、SEQIDNO:22、SEQIDNO:24、SEQIDNO:26和SEQIDNO:28的35B7、95B7、100F11、139F12、157B11、161A1、161C12、189H8、196D9、198E11、251B4、251C4和382A2克隆的酶活性增强。2-2、在Avicel底物上然后在第二种底物：Avicel上测量分别编码酶SEQIDNO:4、SEQIDNO:6、SEQIDNO:8、SEQIDNO:10、SEQIDNO:12、SEQIDNO:14、SEQIDNO:16、SEQIDNO:18、SEQIDNO:20、SEQIDNO:22、SEQIDNO:24、SEQIDNO:26和SEQIDNO:28的35B7、95B7、100F11、139F12、157B11、161A1、161C12、189H8、196D9、198E11、251B4、251C4和382A2克隆的活性的增强。根据以下实验方案通过端点测量进行在这种底物上活性增强的测定：–制备表达根据本发明的重组酶的解脂耶罗维亚酵母的原种培养物，于28℃过夜；–用一定体积的原种培养物接种表达培养基使其在培养开始时在600nm的光密度为0.2；–于28℃培养所述细胞96小时；–在8000rpm离心5分钟；–于35℃和50℃用100μl含有1％Avicel的pH6的0.1M柠檬酸磷酸缓冲液孵育100μl上清液18小时；–取出100μl反应液；–加入100μlDNS试剂；–于100℃孵育5分钟；–在冰上孵育3分钟；–在3000rpm离心10分钟；–用150μl在540nm读取光密度。表3示出了在这些实验条件下，35B7、95B7、100F11、139F12、157B11、161A1、161C12、189H8、196D9、198E11、251B4、251C4和382A2克隆在540nm的OD值(减去阴性对照值之后)以及增强倍数。表3：在Avicel上纤维二糖水解酶活性的增强表3的结果表明，198E11和251B4克隆(分别为SEQIDNo:22和24)在35℃的酶活性与CBH2参考酶(SEQIDNO：2)相比增强，382A2克隆(SEQIDNO:28)在35℃和50℃的酶活性与CBH2参考酶(SEQIDNO：2)相比增强。序列表<110>IFPEnergiesNouvellesetal<120>具有增强活性的外切葡聚糖酶变体及其用途<130>BFF140338<160>35<170>PatentInversion3.5<210>1<211>1413<212>DNA<213>里氏木霉<400>1atgattgtcggcattctcaccacgctggctacgctggccacactcgcagctagtgtgcct60ctagaggagcggcaagcttgctcaagcgtctggggccaatgtggtggccagaattggtcg120ggtccgacttgctgtgcttccggaagcacatgcgtctactccaacgactattactcccag180tgtcttcccggcgctgcaagctcaagctcgtccacgcgcgccgcgtcgacgacttctcga240gtatcccccacaacatcccggtcgagctccgcgacgcctccacctggttctactactacc300agagtacctccagtcggatcgggaaccgctacgtattcaggcaacccttttgttggggtc360actccttgggccaatgcatattacgcctctgaagttagcagcctcgctattcctagcttg420actggagccatggccactgctgcagcagctgtcgcaaaggttccctcttttatgtggcta480gatactcttgacaagacccctctcatggagcaaaccttggccgacatccgcaccgccaac540aagaatggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgcgattgc600gctgcccttgcctcgaatggcgaatactctattgccgatggtggcgtcgccaaatataag660aactatatcgacaccattcgtcaaattgtcgtggaatattccgatatccggaccctcctg720gttattgagcctgactctcttgccaacctggtgaccaacctcggtactccaaagtgtgcc780aatgctcagtcagcctaccttgagtgcatcaactacgccgtcacacagctgaaccttcca840aatgttgcgatgtatttggacgctggccatgcaggatggcttggctggccggcaaaccaa900gacccggccgctcagctatttgcaaatgtttacaagaatgcatcgtctccgagagctctt960cgcggattggcaaccaatgtcgccaactacaacgggtggaacattaccagccccccatcg1020tacacgcaaggcaacgctgtctacaacgagaagctgtacatccacgctattggacctctt1080cttgccaatcacggctggtccaacgccttcttcatcactgatcaaggtcgatcgggaaag1140cagcctaccggacagcaacagtggggagactggtgcaatgtgatcggcaccggatttggt1200attcgcccatccgcaaacactggggactcgttgctggattcgtttgtctgggtcaagcca1260ggcggcgagtgtgacggcaccagcgacagcagtgcgccacgatttgactcccactgtgcg1320ctcccagatgccttgcaaccggcgcctcaagctggtgcttggttccaagcctactttgtg1380cagcttctcacaaacgcaaacccatcgttcctg1413<210>2<211>471<212>PRT<213>里氏木霉<400>2MetIleValGlyIleLeuThrThrLeuAlaThrLeuAlaThrLeuAla151015AlaSerValProLeuGluGluArgGlnAlaCysSerSerValTrpGly202530GlnCysGlyGlyGlnAsnTrpSerGlyProThrCysCysAlaSerGly354045SerThrCysValTyrSerAsnAspTyrTyrSerGlnCysLeuProGly505560AlaAlaSerSerSerSerSerThrArgAlaAlaSerThrThrSerArg65707580ValSerProThrThrSerArgSerSerSerAlaThrProProProGly859095SerThrThrThrArgValProProValGlySerGlyThrAlaThrTyr100105110SerGlyAsnProPheValGlyValThrProTrpAlaAsnAlaTyrTyr115120125AlaSerGluValSerSerLeuAlaIleProSerLeuThrGlyAlaMet130135140AlaThrAlaAlaAlaAlaValAlaLysValProSerPheMetTrpLeu145150155160AspThrLeuAspLysThrProLeuMetGluGlnThrLeuAlaAspIle165170175ArgThrAlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheValVal180185190TyrAspLeuProAspArgAspCysAlaAlaLeuAlaSerAsnGlyGlu195200205TyrSerIleAlaAspGlyGlyValAlaLysTyrLysAsnTyrIleAsp210215220ThrIleArgGlnIleValValGluTyrSerAspIleArgThrLeuLeu225230235240ValIleGluProAspSerLeuAlaAsnLeuValThrAsnLeuGlyThr245250255ProLysCysAlaAsnAlaGlnSerAlaTyrLeuGluCysIleAsnTyr260265270AlaValThrGlnLeuAsnLeuProAsnValAlaMetTyrLeuAspAla275280285GlyHisAlaGlyTrpLeuGlyTrpProAlaAsnGlnAspProAlaAla290295300GlnLeuPheAlaAsnValTyrLysAsnAlaSerSerProArgAlaLeu305310315320ArgGlyLeuAlaThrAsnValAlaAsnTyrAsnGlyTrpAsnIleThr325330335SerProProSerTyrThrGlnGlyAsnAlaValTyrAsnGluLysLeu340345350TyrIleHisAlaIleGlyProLeuLeuAlaAsnHisGlyTrpSerAsn355360365AlaPhePheIleThrAspGlnGlyArgSerGlyLysGlnProThrGly370375380GlnGlnGlnTrpGlyAspTrpCysAsnValIleGlyThrGlyPheGly385390395400IleArgProSerAlaAsnThrGlyAspSerLeuLeuAspSerPheVal405410415TrpValLysProGlyGlyGluCysAspGlyThrSerAspSerSerAla420425430ProArgPheAspSerHisCysAlaLeuProAspAlaLeuGlnProAla435440445ProGlnAlaGlyAlaTrpPheGlnAlaTyrPheValGlnLeuLeuThr450455460AsnAlaAsnProSerPheLeu465470<210>3<211>1353<212>DNA<213>人工序列<220><223>克隆35B7<400>3gcccctcttgttgaggagcgccaggcttgcgctgcccaatgggcccagtgtggtggcttc60agctggaatggtgctacctgctgccagtctggtagctactgcagcaagatcaacgactat120tactctcagtgcattcctggagaaggccccgccacttccaagtcgagcactcttcctgct180tccaccacaacaactcagccaacttccacttcgactgctggaacctcttccactaccaag240cctcctccagctggatcgggaaccgctacgtattcaggcaacccttactctggggtcaac300ctttgggccaatagctattaccgctctgaagttaccaacctcgctattcctaagttgagc360ggagccatggccactgctgcagcaaaggtcgcagatgttccctcttttcagtggatggat420tctttcgaccacatctccctcatggaggacaccttggtcgacatccgcaaggccaacctg480gctggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgcgattgcgct540gccgccgcctcgaatggcgaatactctcttgccgatggtggcgtcgccaaatataaggcc600tatatcgccaagattaagggtattctccaggactattccgatacccggatcattctggtt660attgagcctgactctcttgccaacctggtgaccaacatgaacgtcccaaagtgtgccaat720gctgcttcagcctacaaggagctcaccattcacgccctcaaggagctgaaccttccaaat780gtttccatgtatatcgacgctggccatggtggatggcttggctggccggcaaaccaaggc840ccggccgctaaactatttgcaagcatctacaaggatgcaggcaagccggccgctcttcgc900ggattggcaaccaatgtcgccaactacaacgcctggagcctcagcagcgctccaccttac960acgcaaggcgcctctatctacgacgagaagagcttcatccacgctatgggacctcttctt1020gagcagaatggctggcctggtgcccacttcatcactgatcaaggtcgatcgggaaagcag1080cctaccggacagatccagtggggagactggtgcaattccaagggcaccggatttggtatt1140cgcccatccgcaaacactggggactcgttgctggatgcttttgtctgggtcaagccaggc1200ggcgagtctgacggcaccagcgacaccagtgcggctcgatacgactaccactgtggtatt1260gacggcgccgtcaagccggcgcctgaggctggtacctggttccaagcctactttgagcag1320cttctcacaaacgcaaacccatcgttcctgtaa1353<210>4<211>450<212>PRT<213>人工序列<220><223>克隆35B7<400>4AlaProLeuValGluGluArgGlnAlaCysAlaAlaGlnTrpAlaGln151015CysGlyGlyPheSerTrpAsnGlyAlaThrCysCysGlnSerGlySer202530TyrCysSerLysIleAsnAspTyrTyrSerGlnCysIleProGlyGlu354045GlyProAlaThrSerLysSerSerThrLeuProAlaSerThrThrThr505560ThrGlnProThrSerThrSerThrAlaGlyThrSerSerThrThrLys65707580ProProProAlaGlySerGlyThrAlaThrTyrSerGlyAsnProTyr859095SerGlyValAsnLeuTrpAlaAsnSerTyrTyrArgSerGluValThr100105110AsnLeuAlaIleProLysLeuSerGlyAlaMetAlaThrAlaAlaAla115120125LysValAlaAspValProSerPheGlnTrpMetAspSerPheAspHis130135140IleSerLeuMetGluAspThrLeuValAspIleArgLysAlaAsnLeu145150155160AlaGlyGlyAsnTyrAlaGlyGlnPheValValTyrAspLeuProAsp165170175ArgAspCysAlaAlaAlaAlaSerAsnGlyGluTyrSerLeuAlaAsp180185190GlyGlyValAlaLysTyrLysAlaTyrIleAlaLysIleLysGlyIle195200205LeuGlnAspTyrSerAspThrArgIleIleLeuValIleGluProAsp210215220SerLeuAlaAsnLeuValThrAsnMetAsnValProLysCysAlaAsn225230235240AlaAlaSerAlaTyrLysGluLeuThrIleHisAlaLeuLysGluLeu245250255AsnLeuProAsnValSerMetTyrIleAspAlaGlyHisGlyGlyTrp260265270LeuGlyTrpProAlaAsnGlnGlyProAlaAlaLysLeuPheAlaSer275280285IleTyrLysAspAlaGlyLysProAlaAlaLeuArgGlyLeuAlaThr290295300AsnValAlaAsnTyrAsnAlaTrpSerLeuSerSerAlaProProTyr305310315320ThrGlnGlyAlaSerIleTyrAspGluLysSerPheIleHisAlaMet325330335GlyProLeuLeuGluGlnAsnGlyTrpProGlyAlaHisPheIleThr340345350AspGlnGlyArgSerGlyLysGlnProThrGlyGlnIleGlnTrpGly355360365AspTrpCysAsnSerLysGlyThrGlyPheGlyIleArgProSerAla370375380AsnThrGlyAspSerLeuLeuAspAlaPheValTrpValLysProGly385390395400GlyGluSerAspGlyThrSerAspThrSerAlaAlaArgTyrAspTyr405410415HisCysGlyIleAspGlyAlaValLysProAlaProGluAlaGlyThr420425430TrpPheGlnAlaTyrPheGluGlnLeuLeuThrAsnAlaAsnProSer435440445PheLeu450<210>5<211>1362<212>DNA<213>人工序列<220><223>克隆95B7<400>5gtgcctctagaggagcggcaagcttgctcaagcgtctggggccaatgtggtggccagaat60tggtcgggtccgacttgctgtgcttccggaagcacatgcgtctactccaacgactattac120tcccagtgtcttcccggcgctgcaagctcaagctcgtccacgcgcgccgcgtcgacgact180tctcgagtatcccccacaacatcccggtcgagctccgcgacgcctccacctggttctact240actaccagagtacctccagtcggatcgggaaccgctacgtattcaggcaacccttttgtt300ggggtcactccttgggccaatgcatattacgcctctgaagttagcagcctcgctattcct360agcttgactggagccatggccactgctgcagcagctgtcgcaaaggttccctcttttatg420tggctagatactcttgacaagacccctctcatggagcaaaccttggccgacatccgcacc480gccaacaagaatggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgc540gattgcgctgcccttgcctcgaatggcgaatactctattgccgatggtggcgtcgccaaa600tataagaactatatcgacaccattcgtcaaattgtcgtggaatattccgatatccggacc660ctcctggttattgagcctgactctcttgccaaccaggtgaccaacatggatgtcgccaag720tgtgccaaggctcatgatgcctacatcagcctgacgaactacgccgtcacagaactgaac780cttccaaatgttgcgatgtatttggacgctggcaatgcaggatggcttggctggccggca840aaccaaggcccggccgctaaactatttgcaagcatctacaaggatgcaggcaagccggcc900gctcttcgcggattggcaaccaatgtcgccaactacaacgcctggagcctcagcagcgct960ccaccttacacgcaaggcgcctctatctacgacgagaagagcttcatccacgctatggga1020cctcttcttgagcagaatggctggcctggtgcccacttcatcactgatcaaggtcgatcg1080ggaaagcagcctaccggacagatccagtggggagactggtgcaattccaagggcaccgga1140tttggtattcgcccatccgcaaacactggggactcgttgctggatgcttttgtctgggtc1200aagccaggcggcgagtctgacggcaccagcgacaccagtgcgacccgatacgactaccac1260tgtggtgcttctgccgccttgcaaccggcgcctgaggctggtacctggttccaagcctac1320tttgagcagcttctcaagaacgcaaacccatcgttcctgtaa1362<210>6<211>453<212>PRT<213>人工序列<220><223>克隆95B7<400>6ValProLeuGluGluArgGlnAlaCysSerSerValTrpGlyGlnCys151015GlyGlyGlnAsnTrpSerGlyProThrCysCysAlaSerGlySerThr202530CysValTyrSerAsnAspTyrTyrSerGlnCysLeuProGlyAlaAla354045SerSerSerSerSerThrArgAlaAlaSerThrThrSerArgValSer505560ProThrThrSerArgSerSerSerAlaThrProProProGlySerThr65707580ThrThrArgValProProValGlySerGlyThrAlaThrTyrSerGly859095AsnProPheValGlyValThrProTrpAlaAsnAlaTyrTyrAlaSer100105110GluValSerSerLeuAlaIleProSerLeuThrGlyAlaMetAlaThr115120125AlaAlaAlaAlaValAlaLysValProSerPheMetTrpLeuAspThr130135140LeuAspLysThrProLeuMetGluGlnThrLeuAlaAspIleArgThr145150155160AlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheValValTyrAsp165170175LeuProAspArgAspCysAlaAlaLeuAlaSerAsnGlyGluTyrSer180185190IleAlaAspGlyGlyValAlaLysTyrLysAsnTyrIleAspThrIle195200205ArgGlnIleValValGluTyrSerAspIleArgThrLeuLeuValIle210215220GluProAspSerLeuAlaAsnGlnValThrAsnMetAspValAlaLys225230235240CysAlaLysAlaHisAspAlaTyrIleSerLeuThrAsnTyrAlaVal245250255ThrGluLeuAsnLeuProAsnValAlaMetTyrLeuAspAlaGlyAsn260265270AlaGlyTrpLeuGlyTrpProAlaAsnGlnGlyProAlaAlaLysLeu275280285PheAlaSerIleTyrLysAspAlaGlyLysProAlaAlaLeuArgGly290295300LeuAlaThrAsnValAlaAsnTyrAsnAlaTrpSerLeuSerSerAla305310315320ProProTyrThrGlnGlyAlaSerIleTyrAspGluLysSerPheIle325330335HisAlaMetGlyProLeuLeuGluGlnAsnGlyTrpProGlyAlaHis340345350PheIleThrAspGlnGlyArgSerGlyLysGlnProThrGlyGlnIle355360365GlnTrpGlyAspTrpCysAsnSerLysGlyThrGlyPheGlyIleArg370375380ProSerAlaAsnThrGlyAspSerLeuLeuAspAlaPheValTrpVal385390395400LysProGlyGlyGluSerAspGlyThrSerAspThrSerAlaThrArg405410415TyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnProAlaProGlu420425430AlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeuLysAsnAla435440445AsnProSerPheLeu450<210>7<211>1323<212>DNA<213>人工序列<220><223>克隆100F11<400>7gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccttcatggaggag420tctttggccgacatccgcaaggccaacaaggctggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgctgcctcgaatggcgaatactctctt540gacaaggatggcaagaacaaatataaggcctatatcgccaagattaagggtattctccag600gactattccgatacccggatcattctggttattgagcctgactctcttgccaacatggtg660accaacatgaacgtcccaaagtgtgccaatgctgcttcagcctacaaggagctcaccatt720cacgccctcaaggagctgaaccttccaaatgtttccatgtatatcgacgctggccatggt780ggatggcttggctggccggcaaaccttcctccggccgctcagctatacggtcagctctac840aaggatgcaggcaagccgtctcgccttcgcggattggtcaccaatgtctccaactacaac900gcctggaagctatccagcaagccagactacacggagagcaaccccaactacgacgagcag960aagtacatccacgctctgtctcctcttcttgagcaggagggctggcccggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtattcgcccatccgcaaacactggggactcgttg1140ctggatgcttttgtctgggtcaagccaggcggcgagtctgacggcaccagcgacaccagt1200gcgacccgatacgactaccactgtggtgcttctgccgccttgcaaccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcacaaacgcaaacccatcgttcctg1320taa1323<210>8<211>440<212>PRT<213>人工序列<220><223>克隆100F11<400>8AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerPheMetGluGluSerLeuAlaAsp130135140IleArgLysAlaAsnLysAlaGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspLysAspGlyLysAsnLysTyrLysAlaTyrIle180185190AlaLysIleLysGlyIleLeuGlnAspTyrSerAspThrArgIleIle195200205LeuValIleGluProAspSerLeuAlaAsnMetValThrAsnMetAsn210215220ValProLysCysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIle225230235240HisAlaLeuLysGluLeuAsnLeuProAsnValSerMetTyrIleAsp245250255AlaGlyHisGlyGlyTrpLeuGlyTrpProAlaAsnLeuProProAla260265270AlaGlnLeuTyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArg275280285LeuArgGlyLeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeu290295300SerSerLysProAspTyrThrGluSerAsnProAsnTyrAspGluGln305310315320LysTyrIleHisAlaLeuSerProLeuLeuGluGlnGluGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyIleArgProSerAlaAsnThrGlyAspSerLeuLeuAspAlaPhe370375380ValTrpValLysProGlyGlyGluSerAspGlyThrSerAspThrSer385390395400AlaThrArgTyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430ThrAsnAlaAsnProSerPheLeu435440<210>9<211>1323<212>DNA<213>人工序列<220><223>克隆139F12<400>9gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccttcatggaggag420tctttggccgacatccgcaaggccaacaaggctggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgctgcctcgaatggcgaatactctctt540gacaaggatggcaagaacaaatataaggcctatatcgccaagattaagggtattctccag600gactattccgatacccggatcattctggttattgagcctgactctcttgccaacatggtg660accaacatgaacgtcccaaagtgtgccaatgctgcttcagcctacaaggagctcaccatt720cacgccctcaaggagctgaaccttccaaatgtttccatgtatatcgacgctggccatggt780ggatggcttggctggccggcaaaccttcctccggccgctcagctatacggtcagctctac840aaggatgcaggcaagccgtctcgccttcgcggattggtcaccaatgtctccaactacaac900gcctggaagctatccagcaagccagactacacggagagcaaccccaactacgacgagcag960aagtacatccacgctctgtctcctcttcttgagcaggagggctggcccggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtctccgcccatccgcaaacactggggacgccttg1140gtcgatgcttttgtctgggtcaagccaggcggcgagtctgacggcaccagcgacaccagt1200gcggctcgatacgactaccactgtggtgcttctgccgccttgcaaccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcaagaacgcaaacccatcgttcctg1320taa1323<210>10<211>440<212>PRT<213>人工序列<220><223>克隆139F12<400>10AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerPheMetGluGluSerLeuAlaAsp130135140IleArgLysAlaAsnLysAlaGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspLysAspGlyLysAsnLysTyrLysAlaTyrIle180185190AlaLysIleLysGlyIleLeuGlnAspTyrSerAspThrArgIleIle195200205LeuValIleGluProAspSerLeuAlaAsnMetValThrAsnMetAsn210215220ValProLysCysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIle225230235240HisAlaLeuLysGluLeuAsnLeuProAsnValSerMetTyrIleAsp245250255AlaGlyHisGlyGlyTrpLeuGlyTrpProAlaAsnLeuProProAla260265270AlaGlnLeuTyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArg275280285LeuArgGlyLeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeu290295300SerSerLysProAspTyrThrGluSerAsnProAsnTyrAspGluGln305310315320LysTyrIleHisAlaLeuSerProLeuLeuGluGlnGluGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyLeuArgProSerAlaAsnThrGlyAspAlaLeuValAspAlaPhe370375380ValTrpValLysProGlyGlyGluSerAspGlyThrSerAspThrSer385390395400AlaAlaArgTyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430LysAsnAlaAsnProSerPheLeu435440<210>11<211>1323<212>DNA<213>人工序列<220><223>克隆157B11<400>11gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccttcatggaggag420tctttggccgacatccgcaaggccaacaaggctggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgccgcctcgaatggcgaatactctctc540gacaatgatggcgccaacaaatataaggcctatatcgccaagattaagggtattctccag600gactattccgatacccggatcattctggttattgagcctgactctcttgccaacatggtg660accaacatgaacgtcccaaagtgtgccaatgctgcttcagcctacaaggagctcaccatt720cacgccctcaaggagctgaaccttccaaatgtttccatgtatatcgacgctggccatggt780ggatggcttggctggccggcaaaccttcctccggccgctcagctatacggtcagctctac840aaggatgcaggcaagccgtctcgccttcgcggattggtcaccaatgtctccaactacaac900gcctggaagctatccagcaagccagactacacggagagcaaccccaactacgacgagcag960aagtacatccacgctctgtctcctcttcttgagcaggagggctggcccggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtctccgcccatccgcaaacactggggactcgttg1140ctggatgcttttgtctgggtcaagccaggcggcgagtctgacggcaccagcgacaccagt1200gcgacccgatacgactaccactgtggtgcttctgccgccttgcaaccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcacaaacgcaaacccatcgttcctg1320taa1323<210>12<211>440<212>PRT<213>人工序列<220><223>克隆157B11<400>12AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerPheMetGluGluSerLeuAlaAsp130135140IleArgLysAlaAsnLysAlaGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspAsnAspGlyAlaAsnLysTyrLysAlaTyrIle180185190AlaLysIleLysGlyIleLeuGlnAspTyrSerAspThrArgIleIle195200205LeuValIleGluProAspSerLeuAlaAsnMetValThrAsnMetAsn210215220ValProLysCysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIle225230235240HisAlaLeuLysGluLeuAsnLeuProAsnValSerMetTyrIleAsp245250255AlaGlyHisGlyGlyTrpLeuGlyTrpProAlaAsnLeuProProAla260265270AlaGlnLeuTyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArg275280285LeuArgGlyLeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeu290295300SerSerLysProAspTyrThrGluSerAsnProAsnTyrAspGluGln305310315320LysTyrIleHisAlaLeuSerProLeuLeuGluGlnGluGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyLeuArgProSerAlaAsnThrGlyAspSerLeuLeuAspAlaPhe370375380ValTrpValLysProGlyGlyGluSerAspGlyThrSerAspThrSer385390395400AlaThrArgTyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430ThrAsnAlaAsnProSerPheLeu435440<210>13<211>1323<212>DNA<213>人工序列<220><223>克隆161A1<400>13gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccttcatggaggag420tctttggccgacatccgcaaggccaacaaggctggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgctgcctcgaatggcgaatactctctt540gacaaggatggcaagaacaaatataaggcctatatcgccaagattaagggtattctccag600gactattccgatacccggatcattctggttattgagcctgactctcttgccaacatggtg660accaacatgaacgtcccaaagtgtgccaatgctgcttcagcctacaaggagctcaccatt720cacgccctcaaggagctgaaccttccaaatgtttccatgtatatcgacgctggccatggt780ggatggcttggctggccggcaaaccttcctccggccgctcagctatacggtcagctctac840aaggatgcaggcaagccgtctcgccttcgcggattggtcaccaatgtctccaactacaac900gcctggaagctatccagcaagccagactacacggagagcaaccccaactacgacgagcag960aagtacatccacgctctgtctcctcttcttgagcaggagggctggcccggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtatccgcccatccgcaaacactggggacgccttg1140gtcgatgcttttgtctgggtcaagccaggcggcgagtctgacggcaccagcgacaccagt1200gcgacccgatacgactaccactgtggtgcttctgccgccttgcaaccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcaagaacgcaaacccatcgttcctg1320taa1323<210>14<211>440<212>PRT<213>人工序列<220><223>克隆161A1<400>14AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerPheMetGluGluSerLeuAlaAsp130135140IleArgLysAlaAsnLysAlaGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspLysAspGlyLysAsnLysTyrLysAlaTyrIle180185190AlaLysIleLysGlyIleLeuGlnAspTyrSerAspThrArgIleIle195200205LeuValIleGluProAspSerLeuAlaAsnMetValThrAsnMetAsn210215220ValProLysCysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIle225230235240HisAlaLeuLysGluLeuAsnLeuProAsnValSerMetTyrIleAsp245250255AlaGlyHisGlyGlyTrpLeuGlyTrpProAlaAsnLeuProProAla260265270AlaGlnLeuTyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArg275280285LeuArgGlyLeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeu290295300SerSerLysProAspTyrThrGluSerAsnProAsnTyrAspGluGln305310315320LysTyrIleHisAlaLeuSerProLeuLeuGluGlnGluGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyIleArgProSerAlaAsnThrGlyAspAlaLeuValAspAlaPhe370375380ValTrpValLysProGlyGlyGluSerAspGlyThrSerAspThrSer385390395400AlaThrArgTyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430LysAsnAlaAsnProSerPheLeu435440<210>15<211>1362<212>DNA<213>人工序列<220><223>克隆161C12<400>15gtgcctctagaggagcggcaagcttgctcaagcgtctggggccaatgtggtggccagaat60tggtcgggtccgacttgctgtgcttccggaagcacatgcgtctactccaacgactattac120tcccagtgtcttcccggcgctgcaagctcaagctcgtccacgcgcgccgcgtcgacgact180tctcgagtatcccccacaacatcccggtcgagctccgcgacgcctccacctggttctact240actaccagagtacctccagtcggatcgggaaccgctacgtattcaggcaacccttttgtt300ggggtcactccttgggccaatgcatattacgcctctgaagttagcagcctcgctattcct360agcttgactggagccatggccactgctgcagcagctgtcgcaaaggttccctcttttatg420tggctagatactcttgacaagacccctctcatggagcaaaccttggccgacatccgcacc480gccaacaagaatggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgc540gattgcgctgcccttgcctcgaatggcgaatactctctcgacaatgatggcgccaacaaa600tataagaactatatccaaaccattaagaagattatccagagctattccgatatccggata660ctcctggttattgagcctgactctcttgccaacctggtgaccaacatggatgtcgccaag720tgtgccaaggctcatgatgcctacatcagcctgacgaactacgccgtcacagaactgaac780cttccaaatgttgcgatgtatttggacgctggccatgcaggatggcttggctggccggca840aaccttcctccggccgctcagctatacggtcagctctacaaggatgcaggcaagccgtct900cgccttcgcggattggtcaccaatgtctccaactacaacgcctggaagctatccagcaag960ccagactacacggagagcaaccccaactacgacgagcagaagtacatccacgctctgtct1020cctcttcttgagcaggagggctggcccggtgccaagttcatcgtcgatcaaggtcgatcg1080ggaaagcagcctaccggacagaaggcttggggagactggtgcaatgctcccggcaccgga1140tttggtctccgcccatccgcaaacactggggacgccttggtcgatgcttttgtctgggtc1200aagccaggcggcgagtctgacggcaccagcgacaccagtgcggctcgatacgactaccac1260tgtggtattgacggcgccgtcaagccggcgcctgaggctggtacctggttccaagcctac1320tttgagcagcttctcaagaacgcaaacccatcgttcctgtaa1362<210>16<211>453<212>PRT<213>人工序列<220><223>克隆161C12<400>16ValProLeuGluGluArgGlnAlaCysSerSerValTrpGlyGlnCys151015GlyGlyGlnAsnTrpSerGlyProThrCysCysAlaSerGlySerThr202530CysValTyrSerAsnAspTyrTyrSerGlnCysLeuProGlyAlaAla354045SerSerSerSerSerThrArgAlaAlaSerThrThrSerArgValSer505560ProThrThrSerArgSerSerSerAlaThrProProProGlySerThr65707580ThrThrArgValProProValGlySerGlyThrAlaThrTyrSerGly859095AsnProPheValGlyValThrProTrpAlaAsnAlaTyrTyrAlaSer100105110GluValSerSerLeuAlaIleProSerLeuThrGlyAlaMetAlaThr115120125AlaAlaAlaAlaValAlaLysValProSerPheMetTrpLeuAspThr130135140LeuAspLysThrProLeuMetGluGlnThrLeuAlaAspIleArgThr145150155160AlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheValValTyrAsp165170175LeuProAspArgAspCysAlaAlaLeuAlaSerAsnGlyGluTyrSer180185190LeuAspAsnAspGlyAlaAsnLysTyrLysAsnTyrIleGlnThrIle195200205LysLysIleIleGlnSerTyrSerAspIleArgIleLeuLeuValIle210215220GluProAspSerLeuAlaAsnLeuValThrAsnMetAspValAlaLys225230235240CysAlaLysAlaHisAspAlaTyrIleSerLeuThrAsnTyrAlaVal245250255ThrGluLeuAsnLeuProAsnValAlaMetTyrLeuAspAlaGlyHis260265270AlaGlyTrpLeuGlyTrpProAlaAsnLeuProProAlaAlaGlnLeu275280285TyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArgLeuArgGly290295300LeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeuSerSerLys305310315320ProAspTyrThrGluSerAsnProAsnTyrAspGluGlnLysTyrIle325330335HisAlaLeuSerProLeuLeuGluGlnGluGlyTrpProGlyAlaLys340345350PheIleValAspGlnGlyArgSerGlyLysGlnProThrGlyGlnLys355360365AlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPheGlyLeuArg370375380ProSerAlaAsnThrGlyAspAlaLeuValAspAlaPheValTrpVal385390395400LysProGlyGlyGluSerAspGlyThrSerAspThrSerAlaAlaArg405410415TyrAspTyrHisCysGlyIleAspGlyAlaValLysProAlaProGlu420425430AlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeuLysAsnAla435440445AsnProSerPheLeu450<210>17<211>1323<212>DNA<213>人工序列<220><223>克隆189H8<400>17gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccctcatggaggac420accttggtcgacatccgcaaggccaacctggctggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgctgcctcgaatggcgaatactctctt540gacaaggatggcaagaacaaatataaggcctatatcgccaagattaagggtattctccag600gactattccgatacccggatcattctggttattgagcctgactctcttgccaacatggtg660accaacatgaacgtcccaaagtgtgccaatgctgcttcagcctacaaggagctcaccatt720cacgccctcaaggagctgaaccttccaaatgtttccatgtatatcgacgctggccatggt780ggatggcttggctggccggcaaaccaaggcccggccgctaaactatttgcaagcatctac840aaggatgcaggcaagccggccgctcttcgcggattggcaaccaatgtcgccaactacaac900gcctggagcctcagcagcgctccaccttacacgcaaggcgcctctatctacgacgagaag960agcttcatccacgctatgggacctcttcttgagcagaatggctggcctggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtctccgcccatccgcaaacactggggactcgttg1140ctggatgcttttgtctgggtcaagccaggcggcgagtctgacggcaccagcgacaccagt1200gcgacccgatacgactaccactgtggtgcttctgccgccttgcaaccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcacaaacgcaaacccatcgttcctg1320taa1323<210>18<211>440<212>PRT<213>人工序列<220><223>克隆189H8<400>18AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerLeuMetGluAspThrLeuValAsp130135140IleArgLysAlaAsnLeuAlaGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspLysAspGlyLysAsnLysTyrLysAlaTyrIle180185190AlaLysIleLysGlyIleLeuGlnAspTyrSerAspThrArgIleIle195200205LeuValIleGluProAspSerLeuAlaAsnMetValThrAsnMetAsn210215220ValProLysCysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIle225230235240HisAlaLeuLysGluLeuAsnLeuProAsnValSerMetTyrIleAsp245250255AlaGlyHisGlyGlyTrpLeuGlyTrpProAlaAsnGlnGlyProAla260265270AlaLysLeuPheAlaSerIleTyrLysAspAlaGlyLysProAlaAla275280285LeuArgGlyLeuAlaThrAsnValAlaAsnTyrAsnAlaTrpSerLeu290295300SerSerAlaProProTyrThrGlnGlyAlaSerIleTyrAspGluLys305310315320SerPheIleHisAlaMetGlyProLeuLeuGluGlnAsnGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyLeuArgProSerAlaAsnThrGlyAspSerLeuLeuAspAlaPhe370375380ValTrpValLysProGlyGlyGluSerAspGlyThrSerAspThrSer385390395400AlaThrArgTyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430ThrAsnAlaAsnProSerPheLeu435440<210>19<211>1362<212>DNA<213>人工序列<220><223>克隆196D9<400>19gtgcctctagaggagcggcaagcttgctcaagcgtctggggccaatgtggtggccagaat60tggtcgggtccgacttgctgtgcttccggaagcacatgcgtctactccaacgactattac120tcccagtgtcttcccggcgctgcaagctcaagctcgtccacgcgcgccgcgtcgacgact180tctcgagtatcccccacaacatcccggtcgagctccgcgacgcctccacctggttctact240actaccagagtacctccagtcggatcgggaaccgctacgtattcaggcaacccttttgtt300ggggtcactccttgggccaatgcatattacgcctctgaagttagcagcctcgctattcct360agcttgactggagccatggccactgctgcagcagctgtcgcaaaggttccctcttttatg420tggctagatactcttgacaagacccctctcatggagcaaaccttggccgacatccgcacc480gccaacaagaatggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgc540gattgcgctgcccttgcctcgaatggcgaatactctattgccgatggtggcgtcgccaaa600tataagaactatatcgacaccattcgtcaaattgtcgtggaatattccgatatccggacc660ctcctggttattgagcctgactctcttaccaacctggtgaccaacatgaacgtcccaaag720tgtgccaatgctcagtcagcctaccttgagtgcatcaactacgccgtcacacagctgaac780cttccaaatgttgcgatgtatttggacgctggccatgcaggatggcttggctggccggca840aaccaagacccggccgctcagctatttgcaaatgtttacaagaatgcatcgtctccgaga900gctcttcgcggattggcaaccaatgtcgccaactacaacgggtggaacattaccagcccc960ccatcgtacacgcaaggcaacgctgtctacaacgagaagctgtacatccacgctattgga1020cctcttcttgccaatcacggctggtccaacgccttcttcatcactgatcaaggtcgatcg1080ggaaagcagcctaccggacagcaacagtggggagactggtgcaatgctcccggcaccgga1140tttggtctccgcccatccgcaaacactggggacgccttggtcgatgcttttgtctgggtc1200aagccaggcggcgagtctgacggcaccagcgacaccagtgcggctcgatacgactaccac1260tgtggtattgacggcgccgtcaagccggcgcctgaggctggtacctggttccaagcctac1320tttgagcagcttctcaagaacgcaaacccatcgttcctgtaa1362<210>20<211>453<212>PRT<213>人工序列<220><223>克隆196D9<400>20ValProLeuGluGluArgGlnAlaCysSerSerValTrpGlyGlnCys151015GlyGlyGlnAsnTrpSerGlyProThrCysCysAlaSerGlySerThr202530CysValTyrSerAsnAspTyrTyrSerGlnCysLeuProGlyAlaAla354045SerSerSerSerSerThrArgAlaAlaSerThrThrSerArgValSer505560ProThrThrSerArgSerSerSerAlaThrProProProGlySerThr65707580ThrThrArgValProProValGlySerGlyThrAlaThrTyrSerGly859095AsnProPheValGlyValThrProTrpAlaAsnAlaTyrTyrAlaSer100105110GluValSerSerLeuAlaIleProSerLeuThrGlyAlaMetAlaThr115120125AlaAlaAlaAlaValAlaLysValProSerPheMetTrpLeuAspThr130135140LeuAspLysThrProLeuMetGluGlnThrLeuAlaAspIleArgThr145150155160AlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheValValTyrAsp165170175LeuProAspArgAspCysAlaAlaLeuAlaSerAsnGlyGluTyrSer180185190IleAlaAspGlyGlyValAlaLysTyrLysAsnTyrIleAspThrIle195200205ArgGlnIleValValGluTyrSerAspIleArgThrLeuLeuValIle210215220GluProAspSerLeuThrAsnLeuValThrAsnMetAsnValProLys225230235240CysAlaAsnAlaGlnSerAlaTyrLeuGluCysIleAsnTyrAlaVal245250255ThrGlnLeuAsnLeuProAsnValAlaMetTyrLeuAspAlaGlyHis260265270AlaGlyTrpLeuGlyTrpProAlaAsnGlnAspProAlaAlaGlnLeu275280285PheAlaAsnValTyrLysAsnAlaSerSerProArgAlaLeuArgGly290295300LeuAlaThrAsnValAlaAsnTyrAsnGlyTrpAsnIleThrSerPro305310315320ProSerTyrThrGlnGlyAsnAlaValTyrAsnGluLysLeuTyrIle325330335HisAlaIleGlyProLeuLeuAlaAsnHisGlyTrpSerAsnAlaPhe340345350PheIleThrAspGlnGlyArgSerGlyLysGlnProThrGlyGlnGln355360365GlnTrpGlyAspTrpCysAsnAlaProGlyThrGlyPheGlyLeuArg370375380ProSerAlaAsnThrGlyAspAlaLeuValAspAlaPheValTrpVal385390395400LysProGlyGlyGluSerAspGlyThrSerAspThrSerAlaAlaArg405410415TyrAspTyrHisCysGlyIleAspGlyAlaValLysProAlaProGlu420425430AlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeuLysAsnAla435440445AsnProSerPheLeu450<210>21<211>1362<212>DNA<213>人工序列<220><223>克隆198E11<400>21gtgcctctagaggagcggcaagcttgctcaagcgtctggggccaatgtggtggccagaat60tggtcgggtccgacttgctgtgcttccggaagcacatgcgtctactccaacgactattac120tcccagtgtcttcccggcgctgcaagctcaagctcgtccacgcgcgccgcgtcgacgact180tctcgagtatcccccacaacatcccggtcgagctccgcgacgcctccacctggttctact240actaccagagtacctccagtcggatcgggaaccgctacgtattcaggcaacccttttgtt300ggggtcactccttgggccaatgcatattacgcctctgaagttagcagcctcgctattcct360agcttgactggagccatggccactgctgcagcagctgtcgcaaaggttccctcttttatg420tggctagatactcttgacaagacccctctcatggagcaaaccttggccgacatccgcacc480gccaacaagaatggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgc540gattgcgctgccgctgcctcgaatggcgaatactctcttgacaaggatggcaagaacaaa600tataaggcctatatcgccaagattaagggtattctccaggactattccgatacccggatc660attctggttattgagcctgactctcttgccaacatggtgaccaacatgaacgtcccaaag720tgtgccaatgctgcttcagcctacaaggagctcaccattcacgccctcaaggagctgaac780cttccaaatgtttccatgtatatcgacgctggccatggtggatggcttggctggccggca840aaccttcctccggccgctcagctatacggtcagctctacaaggatgcaggcaagccgtct900cgccttcgcggattggtcaccaatgtctccaactacaacgcctggaagctatccagcaag960ccagactacacggagagcaaccccaactacgacgagcagaagtacatccacgctctgtct1020cctcttcttgagcaggagggctggcccggtgccaagttcatcgtcgatcaaggtcgatcg1080ggaaagcagcctaccggacagaaggcttggggagactggtgcaatgtgatcggcaccgga1140tttggtattcgcccatccgcaaacactggggactcgttgctggattcgtttgtctgggtc1200aagccaggcggcgagtctgacggcaccagcgacaccagtgcgacccgatacgactaccac1260tgtggtgcttctgccgccttgcaaccggcgcctgaggctggtacctggttccaagcctac1320tttgagcagcttctcacaaacgcaaacccatcgttcctgtaa1362<210>22<211>453<212>PRT<213>人工序列<220><223>克隆198E11<400>22ValProLeuGluGluArgGlnAlaCysSerSerValTrpGlyGlnCys151015GlyGlyGlnAsnTrpSerGlyProThrCysCysAlaSerGlySerThr202530CysValTyrSerAsnAspTyrTyrSerGlnCysLeuProGlyAlaAla354045SerSerSerSerSerThrArgAlaAlaSerThrThrSerArgValSer505560ProThrThrSerArgSerSerSerAlaThrProProProGlySerThr65707580ThrThrArgValProProValGlySerGlyThrAlaThrTyrSerGly859095AsnProPheValGlyValThrProTrpAlaAsnAlaTyrTyrAlaSer100105110GluValSerSerLeuAlaIleProSerLeuThrGlyAlaMetAlaThr115120125AlaAlaAlaAlaValAlaLysValProSerPheMetTrpLeuAspThr130135140LeuAspLysThrProLeuMetGluGlnThrLeuAlaAspIleArgThr145150155160AlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheValValTyrAsp165170175LeuProAspArgAspCysAlaAlaAlaAlaSerAsnGlyGluTyrSer180185190LeuAspLysAspGlyLysAsnLysTyrLysAlaTyrIleAlaLysIle195200205LysGlyIleLeuGlnAspTyrSerAspThrArgIleIleLeuValIle210215220GluProAspSerLeuAlaAsnMetValThrAsnMetAsnValProLys225230235240CysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIleHisAlaLeu245250255LysGluLeuAsnLeuProAsnValSerMetTyrIleAspAlaGlyHis260265270GlyGlyTrpLeuGlyTrpProAlaAsnLeuProProAlaAlaGlnLeu275280285TyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArgLeuArgGly290295300LeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeuSerSerLys305310315320ProAspTyrThrGluSerAsnProAsnTyrAspGluGlnLysTyrIle325330335HisAlaLeuSerProLeuLeuGluGlnGluGlyTrpProGlyAlaLys340345350PheIleValAspGlnGlyArgSerGlyLysGlnProThrGlyGlnLys355360365AlaTrpGlyAspTrpCysAsnValIleGlyThrGlyPheGlyIleArg370375380ProSerAlaAsnThrGlyAspSerLeuLeuAspSerPheValTrpVal385390395400LysProGlyGlyGluSerAspGlyThrSerAspThrSerAlaThrArg405410415TyrAspTyrHisCysGlyAlaSerAlaAlaLeuGlnProAlaProGlu420425430AlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeuThrAsnAla435440445AsnProSerPheLeu450<210>23<211>1323<212>DNA<213>人工序列<220><223>克隆251B4<400>23gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccttcatggaggag420tctttggccgacatccgcaccgccaacaagaatggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgccgcctcgaatggcgaatactctctc540gacaatgatggcgccaacaaatataagaactatatccaaaccattaagaagattatccag600agctattccgatatccggatactcctggttattgagcctgactctcttgccaacctggtg660accaacatggatgtcgccaagtgtgccaaggctcatgatgcctacatcagcctgacgaac720tacgccgtcacagaactgaaccttccaaatgttgcgatgtatttggacgctggccatgca780ggatggcttggctggccggcaaaccaaggcccggccgctaaactatttgcaagcatctac840aaggatgcaggcaagccggccgctcttcgcggattggcaaccaatgtcgccaactacaac900gcctggaagctatccagcaagccagactacacggagagcaaccccaactacgacgagcag960aagtacatccacgctctgtctcctcttcttgagcaggagggctggcccggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtctccgcccatccgcaaacactggggacgccttg1140gtcgatgcttttgtctgggtcaagccaggcggcgagtgtgacggcaccagcgacaccagt1200gcggctcgatacgactaccactgtggtattgacggcgccgtcaagccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcacaaacgcaaacccatcgttcctg1320taa1323<210>24<211>440<212>PRT<213>人工序列<220><223>克隆251B4<400>24AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerPheMetGluGluSerLeuAlaAsp130135140IleArgThrAlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspAsnAspGlyAlaAsnLysTyrLysAsnTyrIle180185190GlnThrIleLysLysIleIleGlnSerTyrSerAspIleArgIleLeu195200205LeuValIleGluProAspSerLeuAlaAsnLeuValThrAsnMetAsp210215220ValAlaLysCysAlaLysAlaHisAspAlaTyrIleSerLeuThrAsn225230235240TyrAlaValThrGluLeuAsnLeuProAsnValAlaMetTyrLeuAsp245250255AlaGlyHisAlaGlyTrpLeuGlyTrpProAlaAsnGlnGlyProAla260265270AlaLysLeuPheAlaSerIleTyrLysAspAlaGlyLysProAlaAla275280285LeuArgGlyLeuAlaThrAsnValAlaAsnTyrAsnAlaTrpLysLeu290295300SerSerLysProAspTyrThrGluSerAsnProAsnTyrAspGluGln305310315320LysTyrIleHisAlaLeuSerProLeuLeuGluGlnGluGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyLeuArgProSerAlaAsnThrGlyAspAlaLeuValAspAlaPhe370375380ValTrpValLysProGlyGlyGluCysAspGlyThrSerAspThrSer385390395400AlaAlaArgTyrAspTyrHisCysGlyIleAspGlyAlaValLysPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430ThrAsnAlaAsnProSerPheLeu435440<210>25<211>1362<212>DNA<213>人工序列<220><223>克隆251C4<400>25gtgcctctagaggagcggcaagcttgctcaagcgtctggggccaatgtggtggccagaat60tggtcgggtccgacttgctgtgcttccggaagcacatgcgtctactccaacgactattac120tcccagtgtcttcccggcgctgcaagctcaagctcgtccacgcgcgccgcgtcgacgact180tctcgagtatcccccacaacatcccggtcgagctccgcgacgcctccacctggttctact240actaccagagtacctccagtcggatcgggaaccgctacgtattcaggcaacccttttgtt300ggggtcactccttgggccaatgcatattacgcctctgaagttagcagcctcgctattcct360agcttgactggagccatggccactgctgcagcagctgtcgcaaaggttccctcttttatg420tggctagatactcttgacaagacccctctcatggagcaaaccttggccgacatccgcacc480gccaacaagaatggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgc540gattgcgctgccgctgcctcgaatggcgaatactctattgccgatggtggcgtcgccaaa600tataagaactatatcgacaccattcgtcaaattgtcgtggaatattccgatatccggacc660ctcctggttattgagcctgactctcttgccaacctggtgaccaacctcggtactccaaag720tgtgccaatgctcagtcagcctaccttgagtgcatcaactacgccgtcacacagctgaac780cttccaaatgttgcgatgtatttggacgctggccatgcaggatggcttggctggccggca840aaccaagacccggccgctcagctatttgcaaatgtttacaagaatgcatcgtctccgaga900gctcttcgcggattggcaaccaatgtcgccaactacaacgggtggaacattaccagcccc960ccatcgtacacgcaaggcaacgctgtctacaacgagaagctgtacatccacgctattgga1020cctcttcttgccaatcacggctggtccaacgccttcttcatcactgatcaaggtcgatcg1080ggaaagcagcctaccggacagcaacagtggggagactggtgcaatgtgatcggcaccgga1140tttggtattcgcccatccgcaaacactggggactcgttgctggattcgtttgtctgggtc1200aagccaggcggcgagtgtgacggcaccagcgacaccagtgcgccacgatttgactcccac1260tgtgcgctcccagatgccttgcaaccggcgcctgaggctggtacctggttccaagcctac1320tttgagcagcttctcacaaacgcaaacccatcgttcctgtaa1362<210>26<211>453<212>PRT<213>人工序列<220><223>克隆251C4<400>26ValProLeuGluGluArgGlnAlaCysSerSerValTrpGlyGlnCys151015GlyGlyGlnAsnTrpSerGlyProThrCysCysAlaSerGlySerThr202530CysValTyrSerAsnAspTyrTyrSerGlnCysLeuProGlyAlaAla354045SerSerSerSerSerThrArgAlaAlaSerThrThrSerArgValSer505560ProThrThrSerArgSerSerSerAlaThrProProProGlySerThr65707580ThrThrArgValProProValGlySerGlyThrAlaThrTyrSerGly859095AsnProPheValGlyValThrProTrpAlaAsnAlaTyrTyrAlaSer100105110GluValSerSerLeuAlaIleProSerLeuThrGlyAlaMetAlaThr115120125AlaAlaAlaAlaValAlaLysValProSerPheMetTrpLeuAspThr130135140LeuAspLysThrProLeuMetGluGlnThrLeuAlaAspIleArgThr145150155160AlaAsnLysAsnGlyGlyAsnTyrAlaGlyGlnPheValValTyrAsp165170175LeuProAspArgAspCysAlaAlaAlaAlaSerAsnGlyGluTyrSer180185190IleAlaAspGlyGlyValAlaLysTyrLysAsnTyrIleAspThrIle195200205ArgGlnIleValValGluTyrSerAspIleArgThrLeuLeuValIle210215220GluProAspSerLeuAlaAsnLeuValThrAsnLeuGlyThrProLys225230235240CysAlaAsnAlaGlnSerAlaTyrLeuGluCysIleAsnTyrAlaVal245250255ThrGlnLeuAsnLeuProAsnValAlaMetTyrLeuAspAlaGlyHis260265270AlaGlyTrpLeuGlyTrpProAlaAsnGlnAspProAlaAlaGlnLeu275280285PheAlaAsnValTyrLysAsnAlaSerSerProArgAlaLeuArgGly290295300LeuAlaThrAsnValAlaAsnTyrAsnGlyTrpAsnIleThrSerPro305310315320ProSerTyrThrGlnGlyAsnAlaValTyrAsnGluLysLeuTyrIle325330335HisAlaIleGlyProLeuLeuAlaAsnHisGlyTrpSerAsnAlaPhe340345350PheIleThrAspGlnGlyArgSerGlyLysGlnProThrGlyGlnGln355360365GlnTrpGlyAspTrpCysAsnValIleGlyThrGlyPheGlyIleArg370375380ProSerAlaAsnThrGlyAspSerLeuLeuAspSerPheValTrpVal385390395400LysProGlyGlyGluCysAspGlyThrSerAspThrSerAlaProArg405410415PheAspSerHisCysAlaLeuProAspAlaLeuGlnProAlaProGlu420425430AlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeuThrAsnAla435440445AsnProSerPheLeu450<210>27<211>1353<212>DNA<213>人工序列<220><223>克隆382A2<400>27gcccctcttgttgaggagcgccaggcttgcgctgcccaatgggcccagtgtggtggcttc60agctggaatggtgctacctgctgccagtctggtagctactgcagcaagatcaacgactat120tactctcagtgcattcctggagaaggccccgccacttccaagtcgagcactcttcctgct180tccaccacaacaactcagccaacttccacttcgactgctggaacctcttccactaccaag240cctcctccagctggatcgggaaccgctacgtattcaggcaacccttactctggggtcaac300ctttgggccaatagctattaccgctctgaagttaccaacctcgctattcctaagttgagc360ggagccatggccactgctgcagcaaaggtcgcagatgttccctcttttcagtggatggat420acttatgaccacatctccttcatggaggagtctttggccgacatccgcaaggccaacaag480gctggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgcgattgcgct540gccgctgcctcgaatggcgaatactctcttgacaaggatggcaagaacaaatataaggcc600tatatcgccaagattaagggtattctccaggactattccgatacccggatcattctggtt660attgagcctgactctcttgccaacatggtgaccaacatgaacgtcccaaagtgtgccaat720gctgcttcagcctacaaggagctcaccattcacgccctcaaggagctgaaccttccaaat780gtttccatgtatatcgacgctggccatggtggatggcttggctggccggcaaaccttcct840ccggccgctcagctatacggtcagctctacaaggatgcaggcaagccgtctcgccttcgc900ggattggtcaccaatgtctccaactacaacgcctggaagctatccagcaagccagactac960acggagagcaaccccaactacgacgagcagaagtacatccacgctctgtctcctcttctt1020gagcaggagggctggcccggtgccaagttcatcgtcgatcaaggtcgatcgggaaagcag1080cctaccggacagaaggcttggggagactggtgcaatgctcccggcaccggatttggtctc1140cgcccatccgcaaacactggggacgccttggtcgatgcttttgtctgggtcaagccaggc1200ggcgagtctgacggcaccagcgacaccagtgcggctcgatacgactaccactgtggtatt1260gacggcgccgtcaagccggcgcctgaggctggtacctggttccaagcctactttgagcag1320cttctcaagaacgcaaacccatcgttcctgtaa1353<210>28<211>450<212>PRT<213>人工序列<220><223>克隆382A2<400>28AlaProLeuValGluGluArgGlnAlaCysAlaAlaGlnTrpAlaGln151015CysGlyGlyPheSerTrpAsnGlyAlaThrCysCysGlnSerGlySer202530TyrCysSerLysIleAsnAspTyrTyrSerGlnCysIleProGlyGlu354045GlyProAlaThrSerLysSerSerThrLeuProAlaSerThrThrThr505560ThrGlnProThrSerThrSerThrAlaGlyThrSerSerThrThrLys65707580ProProProAlaGlySerGlyThrAlaThrTyrSerGlyAsnProTyr859095SerGlyValAsnLeuTrpAlaAsnSerTyrTyrArgSerGluValThr100105110AsnLeuAlaIleProLysLeuSerGlyAlaMetAlaThrAlaAlaAla115120125LysValAlaAspValProSerPheGlnTrpMetAspThrTyrAspHis130135140IleSerPheMetGluGluSerLeuAlaAspIleArgLysAlaAsnLys145150155160AlaGlyGlyAsnTyrAlaGlyGlnPheValValTyrAspLeuProAsp165170175ArgAspCysAlaAlaAlaAlaSerAsnGlyGluTyrSerLeuAspLys180185190AspGlyLysAsnLysTyrLysAlaTyrIleAlaLysIleLysGlyIle195200205LeuGlnAspTyrSerAspThrArgIleIleLeuValIleGluProAsp210215220SerLeuAlaAsnMetValThrAsnMetAsnValProLysCysAlaAsn225230235240AlaAlaSerAlaTyrLysGluLeuThrIleHisAlaLeuLysGluLeu245250255AsnLeuProAsnValSerMetTyrIleAspAlaGlyHisGlyGlyTrp260265270LeuGlyTrpProAlaAsnLeuProProAlaAlaGlnLeuTyrGlyGln275280285LeuTyrLysAspAlaGlyLysProSerArgLeuArgGlyLeuValThr290295300AsnValSerAsnTyrAsnAlaTrpLysLeuSerSerLysProAspTyr305310315320ThrGluSerAsnProAsnTyrAspGluGlnLysTyrIleHisAlaLeu325330335SerProLeuLeuGluGlnGluGlyTrpProGlyAlaLysPheIleVal340345350AspGlnGlyArgSerGlyLysGlnProThrGlyGlnLysAlaTrpGly355360365AspTrpCysAsnAlaProGlyThrGlyPheGlyLeuArgProSerAla370375380AsnThrGlyAspAlaLeuValAspAlaPheValTrpValLysProGly385390395400GlyGluSerAspGlyThrSerAspThrSerAlaAlaArgTyrAspTyr405410415HisCysGlyIleAspGlyAlaValLysProAlaProGluAlaGlyThr420425430TrpPheGlnAlaTyrPheGluGlnLeuLeuLysAsnAlaAsnProSer435440445PheLeu450<210>29<211>1323<212>DNA<213>小麦赤霉菌<400>29gctcctgttgaagagcgtcagtcttgcagcaacggagtctggtctcaatgtggtggtcag60aactggtccggtactccttgctgcaccagtggaaacaagtgtgtcaaggtcaacgacttc120tactcccagtgccagcctggatctgcagacccttctcccacatccaccattgtctccgcg180acgaccaccaaggctactactactggtagtggaggctctgtcacctcgcctcctcctgtt240gccaccaacaaccctttttctggggtcgatctgtgggccaataactattaccgctctgaa300gttagcactctcgctattcctaagttgagcggagccatggccactgctgcagcaaaggtc360gcagatgttccctcttttcagtggatggatacttatgaccacatctccttcatggaggag420tctttggccgacatccgcaaggccaacaaggctggcggtaactatgccggacagtttgtg480gtgtatgacttgccggatcgcgattgcgctgccgctgcctcgaatggcgaatactctctt540gacaaggatggcaagaacaaatataaggcctatatcgccaagattaagggtattctccag600gactattccgatacccggatcattctggttattgagcctgactctcttgccaacatggtg660accaacatgaacgtcccaaagtgtgccaatgctgcttcagcctacaaggagctcaccatt720cacgccctcaaggagctgaaccttccaaatgtttccatgtatatcgacgctggccatggt780ggatggcttggctggccggcaaaccttcctccggccgctcagctatacggtcagctctac840aaggatgcaggcaagccgtctcgccttcgcggattggtcaccaatgtctccaactacaac900gcctggaagctatccagcaagccagactacacggagagcaaccccaactacgacgagcag960aagtacatccacgctctgtctcctcttcttgagcaggagggctggcccggtgccaagttc1020atcgtcgatcaaggtcgatcgggaaagcagcctaccggacagaaggcttggggagactgg1080tgcaatgctcccggcaccggatttggtctccgcccatccgcaaacactggggacgccttg1140gtcgatgcttttgtctgggtcaagccaggcggcgagtctgacggcaccagcgacaccagt1200gcggctcgatacgactaccactgtggtattgacggcgccgtcaagccggcgcctgaggct1260ggtacctggttccaagcctactttgagcagcttctcaagaacgcaaacccatcgttcctg1320taa1323<210>30<211>440<212>PRT<213>小麦赤霉菌<400>30AlaProValGluGluArgGlnSerCysSerAsnGlyValTrpSerGln151015CysGlyGlyGlnAsnTrpSerGlyThrProCysCysThrSerGlyAsn202530LysCysValLysValAsnAspPheTyrSerGlnCysGlnProGlySer354045AlaAspProSerProThrSerThrIleValSerAlaThrThrThrLys505560AlaThrThrThrGlySerGlyGlySerValThrSerProProProVal65707580AlaThrAsnAsnProPheSerGlyValAspLeuTrpAlaAsnAsnTyr859095TyrArgSerGluValSerThrLeuAlaIleProLysLeuSerGlyAla100105110MetAlaThrAlaAlaAlaLysValAlaAspValProSerPheGlnTrp115120125MetAspThrTyrAspHisIleSerPheMetGluGluSerLeuAlaAsp130135140IleArgLysAlaAsnLysAlaGlyGlyAsnTyrAlaGlyGlnPheVal145150155160ValTyrAspLeuProAspArgAspCysAlaAlaAlaAlaSerAsnGly165170175GluTyrSerLeuAspLysAspGlyLysAsnLysTyrLysAlaTyrIle180185190AlaLysIleLysGlyIleLeuGlnAspTyrSerAspThrArgIleIle195200205LeuValIleGluProAspSerLeuAlaAsnMetValThrAsnMetAsn210215220ValProLysCysAlaAsnAlaAlaSerAlaTyrLysGluLeuThrIle225230235240HisAlaLeuLysGluLeuAsnLeuProAsnValSerMetTyrIleAsp245250255AlaGlyHisGlyGlyTrpLeuGlyTrpProAlaAsnLeuProProAla260265270AlaGlnLeuTyrGlyGlnLeuTyrLysAspAlaGlyLysProSerArg275280285LeuArgGlyLeuValThrAsnValSerAsnTyrAsnAlaTrpLysLeu290295300SerSerLysProAspTyrThrGluSerAsnProAsnTyrAspGluGln305310315320LysTyrIleHisAlaLeuSerProLeuLeuGluGlnGluGlyTrpPro325330335GlyAlaLysPheIleValAspGlnGlyArgSerGlyLysGlnProThr340345350GlyGlnLysAlaTrpGlyAspTrpCysAsnAlaProGlyThrGlyPhe355360365GlyLeuArgProSerAlaAsnThrGlyAspAlaLeuValAspAlaPhe370375380ValTrpValLysProGlyGlyGluSerAspGlyThrSerAspThrSer385390395400AlaAlaArgTyrAspTyrHisCysGlyIleAspGlyAlaValLysPro405410415AlaProGluAlaGlyThrTrpPheGlnAlaTyrPheGluGlnLeuLeu420425430LysAsnAlaAsnProSerPheLeu435440<210>31<211>1353<212>DNA<213>鞭毛藻丛赤壳菌<400>31gcccctcttgttgaggagcgccaggcttgcgctgcccaatgggcccagtgtggtggcttc60agctggaatggtgctacctgctgccagtctggtagctactgcagcaagatcaacgactat120tactctcagtgcattcctggagaaggccccgccacttccaagtcgagcactcttcctgct180tccaccacaacaactcagccaacttccacttcgactgctggaacctcttccactaccaag240cctcctccagctggatcgggaaccgctacgtattcaggcaacccttactctggggtcaac300ctttgggccaatagctattaccgctctgaagttaccaacctcgctattcctaagttgagc360ggagccatggccactgctgcagcaaaggtcgcagatgttccctcttatcagtggatggat420tctttcgaccacatctccctcatggaggacaccttggtcgacatccgcaaggccaacctg480gctggcggtaactatgccggacagtttgtggtgtatgacttgccggatcgcgattgcgct540gccgccgcctcgaatggcgaatactctctcgacaatgatggcgccaacaaatataagaac600tatatccaaaccattaagaagattatccagagctattccgatatccggatactcctggtt660attgagcctgactctcttgccaacctggtgaccaacatggatgtcgccaagtgtgccaag720gctcatgatgcctacatcagcctgacgaactacgccgtcacagaactgaaccttccaaat780gttgcgatgtatttggacgctggccatgcaggatggcttggctggccggcaaaccaaggc840ccggccgctaaactatttgcaagcatctacaaggatgcaggcaagccggccgctcttcgc900ggattggcaaccaatgtcgccaactacaacgcctggagcctcagcagcgctccaccttac960acgcaaggcgcctctatctacgacgagaagagcttcatccacgctatgggacctcttctt1020gagcagaatggctggcctggtgcccacttcatcactgatcaaggtcgatcgggaaagcag1080cctaccggacagatccagtggggagactggtgcaattccaagggcaccggatttggtatt1140cgcccatccgcaaacactggggactcgttgctggatgcttttgtctgggtcaagccaggc1200ggcgagtctgacggcaccagcgacaccagtgcgacccgatacgactaccactgtggtgct1260tctgccgccttgcaaccggcgcctgaggctggtacctggttccaagcctactttgagcag1320cttctcacaaacgcaaacccatcgttcctgtaa1353<210>32<211>450<212>PRT<213>鞭毛藻丛赤壳菌<400>32AlaProLeuValGluGluArgGlnAlaCysAlaAlaGlnTrpAlaGln151015CysGlyGlyPheSerTrpAsnGlyAlaThrCysCysGlnSerGlySer202530TyrCysSerLysIleAsnAspTyrTyrSerGlnCysIleProGlyGlu354045GlyProAlaThrSerLysSerSerThrLeuProAlaSerThrThrThr505560ThrGlnProThrSerThrSerThrAlaGlyThrSerSerThrThrLys65707580ProProProAlaGlySerGlyThrAlaThrTyrSerGlyAsnProTyr859095SerGlyValAsnLeuTrpAlaAsnSerTyrTyrArgSerGluValThr100105110AsnLeuAlaIleProLysLeuSerGlyAlaMetAlaThrAlaAlaAla115120125LysValAlaAspValProSerTyrGlnTrpMetAspSerPheAspHis130135140IleSerLeuMetGluAspThrLeuValAspIleArgLysAlaAsnLeu145150155160AlaGlyGlyAsnTyrAlaGlyGlnPheValValTyrAspLeuProAsp165170175ArgAspCysAlaAlaAlaAlaSerAsnGlyGluTyrSerLeuAspAsn180185190AspGlyAlaAsnLysTyrLysAsnTyrIleGlnThrIleLysLysIle195200205IleGlnSerTyrSerAspIleArgIleLeuLeuValIleGluProAsp210215220SerLeuAlaAsnLeuValThrAsnMetAspValAlaLysCysAlaLys225230235240AlaHisAspAlaTyrIleSerLeuThrAsnTyrAlaValThrGluLeu245250255AsnLeuProAsnValAlaMetTyrLeuAspAlaGlyHisAlaGlyTrp260265270LeuGlyTrpProAlaAsnGlnGlyProAlaAlaLysLeuPheAlaSer275280285IleTyrLysAspAlaGlyLysProAlaAlaLeuArgGlyLeuAlaThr290295300AsnValAlaAsnTyrAsnAlaTrpSerLeuSerSerAlaProProTyr305310315320ThrGlnGlyAlaSerIleTyrAspGluLysSerPheIleHisAlaMet325330335GlyProLeuLeuGluGlnAsnGlyTrpProGlyAlaHisPheIleThr340345350AspGlnGlyArgSerGlyLysGlnProThrGlyGlnIleGlnTrpGly355360365AspTrpCysAsnSerLysGlyThrGlyPheGlyIleArgProSerAla370375380AsnThrGlyAspSerLeuLeuAspAlaPheValTrpValLysProGly385390395400GlyGluSerAspGlyThrSerAspThrSerAlaThrArgTyrAspTyr405410415HisCysGlyAlaSerAlaAlaLeuGlnProAlaProGluAlaGlyThr420425430TrpPheGlnAlaTyrPheGluGlnLeuLeuThrAsnAlaAsnProSer435440445PheLeu450<210>33<211>54<212>DNA<213>人工序列<220><223>CBH2的肽信号<400>33atgattgtcggcattctcaccacgctggctacgctggccacactcgcagctagt54<210>34<211>99<212>DNA<213>酿酒酵母<400>34atgaagctttccaccatccttttcacagcctgcgctaccctggctgccgccctcccttcc60cccatcactccttctgaggccgcagttctgcagaagagg99<210>35<211>33<212>PRT<213>酿酒酵母<400>35MetLysLeuSerThrIleLeuPheThrAlaCysAlaThrLeuAlaAla151015AlaLeuProSerProIleThrProSerGluAlaAlaValLeuGlnLys202530Arg当前第1页1 2 3