转C4光合关键基因提高C3植物光合作用的方法与流程

本发明属于植物光合作用改进
技术领域：
，涉及一种转C4光合关键基因提高C3植物光合作用的方法，特别涉及利用多基因转化系统，改造获得一个含5个光合基因的载体，利用不同的启动子驱动这些基因，创制可以显著提高光合作用效率的水稻材料的方法。
背景技术：
：随着工业化、城镇化的发展以及人口的增加，我国粮食需求将呈刚性增长，而耕地减少、水资源短缺、气候变化等对粮食生产的约束日益突出，我国粮食供需将长期处于“紧平衡”状态，保障粮食安全面临严峻挑战。农作物的株型育种和杂种优势利用为我国粮食作物产量的提高，保障国民经济的快速发展做出了巨大贡献，但粮食产量的进一步提高难度越来越大。在当前保障粮食安全作为我国长期国策的背景下，如何实现粮食作物产量水平的持续提升是横亘在我国社会经济、农业科技前进道路上的一道难题。作物收获指数是衡量作物产量的主要指标之一，目前水稻、小麦的收获指数都接近0.5，这意味着这两种作物的粮食产量占全部生物量的近50％，通过传统育种实现粮食产量的大跨度提高已不可能。幸运的是，世界上还有另一些植物(C4植物，如玉米、高粱和甘蔗等)，它们的光合效率要比C3植物高出50％。利用转基因的手段，理论上转化可以显著地提高C4光合作用。尽管C4途径的酶及转运蛋白在C3植物中的过表达研究取得了很大进展，在C3植物叶片的特定区域高水平表达C4途径酶的技术也很成熟，由于光合系统是一个复杂、分工明细的系统，转单个基因并不能明显提高C3植物的光合效率。一些研究表明，高水平表达C4基因对C3植物光合效率提高和生理性状改良的作用有限，有时甚至是不利的。Agarie等测定了PEPC活性高于对照50倍的转基因水稻株系，结果是其光合速率均略低于对照。Ku等发现部分转PEPC水稻植株显示了不良的农艺性状，如株高矮化、叶片变窄、不育等。而试图将PEPC、NADP-ME、PPDK、NADP-MDH全部转入水稻中，以期构成一个完整C4循环的实验也以失败告终，该转多基因水稻同样表现生长受抑制，说明体内所建立的是一个无效的循环，对C4特有的CO2浓缩机制没有贡献。技术实现要素：针对现有技术的不足，本发明采用了不同的启动子策略，将5个C4光合关键基因导入水稻。第一，本发明要求保护一种增强植物光合作用和/或提高植物产量和/或提高植物生物量的方法。本发明所提供的增强植物光合作用和/或提高植物产量和/或提高植物生物量的方法(或称为培育光合作用增强和/或产量提高和/或生物量提高的转基因植物方法)，具体可包括如下步骤：向受体植物中导入5个C4光合关键基因，得到转基因植物；所述转基因植物与所述受体植物相比，光合作用增强和/或产量提高和/或生物量提高。其中，所述5个C4光合关键基因为磷酸丙酮酸双激酶(ZmPPDK1)的编码基因、PEP羧化酶(ZmPEPC1)的编码基因、苹果酸脱氢酶(ZmMDH6)的编码基因、NADP－苹果酸酶(ZmME2)的编码基因，以及碳酸酐酶(ZmCA1)的编码基因。在所述转基因植物中，所述磷酸丙酮酸双激酶(ZmPPDK1)的编码基因由所述磷酸丙酮酸双激酶(ZmPPDK1)的编码基因的内源启动子驱动转录；所述PEP羧化酶(ZmPEPC1)的编码基因由所述PEP羧化酶(ZmPEPC1)的编码基因的内源启动子驱动转录；所述苹果酸脱氢酶(ZmMDH6)的编码基因由35CaMVS启动子驱动转录；所述NADP－苹果酸酶(ZmME2)的编码基因由Ubiqutin启动子驱动转录；所述碳酸酐酶(ZmCA1)的编码基因由OsActin启动子驱动转录。其中，所述5个C4光合关键基因是通过重组载体的形式导入所述受体植物中的。所述重组载体为下文任一所述的重组载体。在上述方法中，将所述重组载体导入所述受体植物具体可为：通过使用Ti质粒、Ri质粒、植物病毒载体、直接DNA转化、显微注射、电导、农杆菌介导等常规生物学方法转化植物细胞或组织，并将转化的植物组织培育成植株。第二，本发明要求保护一种重组载体或含有所述重组载体的重组菌。所述重组载体为含有DNA片段1、DNA片段2、DNA片段3、DNA片段4和DNA片段5的环形质粒。所述DNA片段1自上游到下游依次含有磷酸丙酮酸双激酶(ZmPPDK1)的编码基因的内源启动子、磷酸丙酮酸双激酶(ZmPPDK1)的编码基因。所述DNA片段2自上游到下游依次含有PEP羧化酶(ZmPEPC1)的编码基因的内源启动子、PEP羧化酶(ZmPEPC1)的编码基因。所述DNA片段3自上游到下游依次含有35CaMVS启动子、苹果酸脱氢酶(ZmMDH6)的编码基因。所述DNA片段4自上游到下游依次含有Ubiqutin启动子、NADP－苹果酸酶(ZmME2)的编码基因。所述DNA片段5自上游到下游依次含有OsActin启动子、碳酸酐酶(ZmCA1)的编码基因。进一步地，所述重组载体为将所述DNA片段1、所述DNA片段2、所述DNA片段3、所述DNA片段4和所述DNA片段5顺次连接后插入到pCambia1300载体的酶切位点HindIII和SmaI之间后得到的重组质粒。前文所述的磷酸丙酮酸双激酶(ZmPPDK1)可为如下任一所示蛋白质：(A1)氨基酸序列为SEQIDNo.11的蛋白质；(A2)将SEQIDNo.11所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加且具有相同功能的蛋白质；(A3)与(A1)-(A2)中任一所限定的氨基酸序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且具有相同功能的蛋白质；(A4)在(A1)-(A3)中任一所限定的蛋白质的N端和/或C端连接标签后得到的融合蛋白。前文所述的PEP羧化酶(ZmPEPC1)可为如下任一所示蛋白质：(B1)氨基酸序列为SEQIDNo.12的蛋白质；(B2)将SEQIDNo.12所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加且具有相同功能的蛋白质；(B3)与(B1)-(B2)中任一所限定的氨基酸序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且具有相同功能的蛋白质；(B4)在(B1)-(B3)中任一所限定的蛋白质的N端和/或C端连接标签后得到的融合蛋白。前文所述的苹果酸脱氢酶(ZmMDH6)可为如下任一所示蛋白质：(C1)氨基酸序列为SEQIDNo.13的蛋白质；(C2)将SEQIDNo.13所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加且具有相同功能的蛋白质；(C3)与(C1)-(C2)中任一所限定的氨基酸序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且具有相同功能的蛋白质；(C4)在(C1)-(C3)中任一所限定的蛋白质的N端和/或C端连接标签后得到的融合蛋白。前文所述的NADP－苹果酸酶(ZmME2)可为如下任一所示蛋白质：(D1)氨基酸序列为SEQIDNo.14的蛋白质；(D2)将SEQIDNo.14所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加且具有相同功能的蛋白质；(D3)与(D1)-(D2)中任一所限定的氨基酸序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且具有相同功能的蛋白质；(D4)在(D1)-(D3)中任一所限定的蛋白质的N端和/或C端连接标签后得到的融合蛋白。前文所述的碳酸酐酶(ZmCA1)可为如下任一所示蛋白质：(E1)氨基酸序列为SEQIDNo.15的蛋白质；(E2)将SEQIDNo.15所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加且具有相同功能的蛋白质；(E3)与(E1)-(E2)中任一所限定的氨基酸序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且具有相同功能的蛋白质；(E4)在(E1)-(E3)中任一所限定的蛋白质的N端和/或C端连接标签后得到的融合蛋白。前文所述的磷酸丙酮酸双激酶(ZmPPDK1)的编码基因可为如下任一所述的DNA分子：(a1)SEQIDNo.2所示的DNA分子；(a2)在严格条件下与(a1)限定的DNA分子杂交且编码所述磷酸丙酮酸双激酶(ZmPPDK1)的DNA分子；(a3)与(a1)-(a2)中任一限定的DNA序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且编码所述磷酸丙酮酸双激酶(ZmPPDK1)的DNA分子。前文所述的PEP羧化酶(ZmPEPC1)的编码基因可为如下任一所述的DNA分子：(b1)SEQIDNo.4所示的DNA分子；(b2)在严格条件下与(b1)限定的DNA分子杂交且编码所述PEP羧化酶(ZmPEPC1)的DNA分子；(b3)与(b1)-(b2)中任一限定的DNA序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且编码所述PEP羧化酶(ZmPEPC1)的DNA分子。前文所述的苹果酸脱氢酶(ZmMDH6)的编码基因可为如下任一所述的DNA分子：(c1)SEQIDNo.6所示的DNA分子；(c2)在严格条件下与(c1)限定的DNA分子杂交且编码所述苹果酸脱氢酶(ZmMDH6)的DNA分子；(c3)与(c1)-(c2)中任一限定的DNA序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且编码所述苹果酸脱氢酶(ZmMDH6)的DNA分子。前文所述的NADP－苹果酸酶(ZmME2)的编码基因可为如下任一所述的DNA分子：(d1)SEQIDNo.8所示的DNA分子；(d2)在严格条件下与(d1)限定的DNA分子杂交且编码所述NADP－苹果酸酶(ZmME2)的DNA分子；(d3)与(d1)-(d2)中任一限定的DNA序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且编码所述NADP－苹果酸酶(ZmME2)的DNA分子。前文所述的碳酸酐酶(ZmCA1)的编码基因可为如下任一所述的DNA分子：(e1)SEQIDNo.10所示的DNA分子；(e2)在严格条件下与(e1)限定的DNA分子杂交且编码所述碳酸酐酶(ZmCA1)的DNA分子；(e3)与(e1)-(e2)中任一限定的DNA序列具有99％以上、95％以上、90％以上、85％以上或者80％以上同源性且编码所述碳酸酐酶(ZmCA1)的DNA分子。上述严格条件可为用6×SSC，0.5％SDS的溶液，在65℃下杂交，然后用2×SSC，0.1％SDS和1×SSC，0.1％SDS各洗膜一次。前文所述的磷酸丙酮酸双激酶(ZmPPDK1)的编码基因的内源启动子的核苷酸序列如SEQIDNo.1所示。前文所述的PEP羧化酶(ZmPEPC1)的编码基因的内源启动子的核苷酸序列如SEQIDNo.3所示。前文所述的35CaMVS启动子的核苷酸序列如SEQIDNo.5所示。前文所述的Ubiqutin启动子的核苷酸序列如SEQIDNo.7所示。前文所述的OsActin启动子的核苷酸序列如SEQIDNo.9所示。更进一步地，所述DNA片段1的核苷酸序列为将SEQIDNo.1和SEQIDNo.2直接顺次连接后得到的序列。所述DNA片段2的核苷酸序列为将SEQIDNo.3和SEQIDNo.4直接顺次连接后得到的序列。所述DNA片段3的核苷酸序列为将SEQIDNo.5和SEQIDNo.6直接顺次连接后得到的序列。所述DNA片段4的核苷酸序列为将SEQIDNo.7和SEQIDNo.8直接顺次连接后得到的序列。所述DNA片段5的核苷酸序列为将SEQIDNo.9和SEQIDNo.10直接顺次连接后得到的序列。在本发明的一个实施例中，所述重组载体具体为将SEQIDNo.1-10所示的10个序列直接顺次连接后插入到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处后得到的重组质粒。第三，本发明要求保护所述重组载体或重组菌在增强植物光合作用和/或提高植物产量和/或提高植物生物量中的应用。在本发明中，所述植物为C3植物。具体的，所述C3植物可为水稻；更加具体的，如水稻品种日本晴(OryzasativaL.ssp.Japonica)。在本发明中，所述增强植物光合作用(或所述光合作用增强)具体可体现为提高植物光合速率。所述提高植物产量(或所述产量提高)具体可体现为提高植物种子千粒重。本发明利用不同启动子驱动5个光合途径的关键蛋白的编码基因，5个基因来源于玉米(Zeamays.L)，分别为磷酸丙酮酸双激酶(ZmPPDK1)、PEP羧化酶(ZmPEPC1)、苹果酸脱氢酶(ZmMDH6)、NADP－苹果酸酶(ZmME2)、碳酸酐酶(ZmCA1)。不同启动子分别为35CaMVS、Ubiqutin、OsActin、ZmPPDK1启动子、ZmPEPC1启动子。将这个多基因载体命名为MGTV(Multi-gene-transformation-vector)载体。将MGTV载体导入C3植物水稻中，创制出具有光合效率显著提高的材料，大辐提高植物的生物量，且没有附带其它不利性状。本发明对于培育C4水稻材料具有重大生产应用价值。附图说明图1为MGTV载体的构建流程图。图2为空载对照和转多基因阳性株系的T1代植株表型。pCambia1300为空载对照植株；MGTV-OV4为转多基因阳性株系OV4。具体实施方式下述实施例中所使用的实验方法如无特殊说明，均为常规方法。下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。下述实施例中的定量试验，均设置三次重复实验，结果取平均值。水稻品种日本晴(OryzasativaL.ssp.Japonica)，又称野生型，用WT表示：中国水稻杂交育种中心(中国，长沙)。pCambia1300载体、pCambia1302载体、pCambia1390载体：pCAMBIA公司。农杆菌AGL1：中国质粒载体菌株细胞株基因保藏中心。实施例1、MGTV载体构建及转基因水稻的获得与功能鉴定一、5个光合元件的克隆及多基因载体构建C4植物玉米比C3植物水稻有着较高的光合效率、生物学产量等，主要原因是C4植物具有C4光合循环途径，本发明从玉米中克隆了这些光合途径关键基因：磷酸丙酮酸双激酶(ZmPPDK1)、PEP羧化酶(ZmPEPC1)、苹果酸脱氢酶(ZmMDH6)、NADP－苹果酸酶(ZmME2)、碳酸酐酶(ZmCA1)。利用不同启动子驱动，其中ZmPPDK1启动子驱动ZmPPDK1，ZmPEPC1启动子驱动ZmPEPC1，35CaMVS驱动ZmMDH6，Ubiqutin启动子驱动ZmME2，OsACTIN启动子驱动ZmCA1。磷酸丙酮酸双激酶(ZmPPDK1)的编码基因(CDS)序列如SEQIDNo.2所示，编码SEQIDNo.11所示蛋白质。PEP羧化酶(ZmPEPC1)的编码基因(CDS)序列如SEQIDNo.4所示，编码SEQIDNo.12所示蛋白质。苹果酸脱氢酶(ZmMDH6)的编码基因(CDS)序列如SEQIDNo.6所示，编码SEQIDNo.13所示蛋白质。NADP－苹果酸酶(ZmME2)的编码基因(CDS)序列如SEQIDNo.8所示，编码SEQIDNo.14所示蛋白质。碳酸酐酶(ZmCA1)的编码基因(CDS)序列如SEQIDNo.10所示，编码SEQIDNo.15所示蛋白质。ZmPPDK1启动子的核苷酸序列如SEQIDNo.1所示。ZmPEPC1启动子的核苷酸序列如SEQIDNo.3所示。35CaMVS启动子的核苷酸序列如SEQIDNo.5所示。Ubiqutin启动子的核苷酸序列如SEQIDNo.7所示。OsActin启动子的核苷酸序列如SEQIDNo.9所示。1、首先分别扩增5个光合基因元件的编码框(CDS)和启动子序列。采用TOYOBOKOD高保真酶扩增，按照说明书进行操作，反应系统如表1所示。表1扩增5个光合基因元件的编码框(CDS)和启动子序列的反应系统对分子量正确的扩增条带切胶回收，-20℃保存产物。2、拼接分别获得5个完整的元件序列。因为用于扩增每组元件CDS的F端引物，加有和启动子重叠的15-18bp序列，因而可以利用融合PCR把每组元件的启动子和CDS拼接为一条PCR产物。同时，为了下一步把5个元件重组到目的载体上，这一轮PCR扩增需要在各自的F端引物增加重叠序列，扩增体系如表2所示。表2拼接分别获得5个完整的元件序列的扩增体系使用Clontech的高效重组酶In-FusionHDCloningKit(货号639648)，利用每个元件之间的15-18bp重复序列、元件1至元件5和pCambia1300载体之间的重复序列，按照表3中所示的对应比例进行重组反应、转化、鉴定、测序，最终完成载体的构建。表3元件1至元件5和pCambia1300载体的反应比例元件编号片段大小所需量体积元件14844bp216ng1.5μl元件24913bp219ng1.5μl元件31837bp82ng0.5μl元件43982bp177ng1μl元件52784bp124ng1μlpCambia1300载体8958bp200ng2μl补2.5μl重组酶至10μl以上所涉及的各引物序列具体如表4所示。表4载体构建所用的引物通过对在筛选培养基上长出的900多个克隆进行PCR鉴定，获得6个重组完全正确的克隆。对该5个克隆进行测序验证，发现构建载体和预期序列一致，并无碱基突变，可以用于下一步转化水稻。将这个多基因载体命名为MGTV(Multi-gene-transformation-vector)载体，见图1。MGTV载体的结构描述：将SEQIDNo.1-10所示的10个序列分别拼接为5个元件，然后利用其中间的重合序列，在重组酶作用下把该5个元件同时重组到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处(位于酶切位点HindIII和SmaI之间)后得到的重组质粒。另外，本发明还构建了如下5个用于转单基因的重组载体：(1)pCambia1300-PPDK1载体：将SEQIDNo.1和SEQIDNo.2所示的2个序列直接顺次连接后插入到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处后得到的重组质粒。(2)pCambia1300-PEPC1载体：将SEQIDNo.3和SEQIDNo.4所示的2个序列直接顺次连接后插入到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处后得到的重组质粒。(3)pCambia1300-MDH6载体：将SEQIDNo.5和SEQIDNo.6所示的2个序列直接顺次连接后插入到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处后得到的重组质粒。(4)pCambia1300-ME2载体：将SEQIDNo.7和SEQIDNo.8所示的2个序列直接顺次连接后插入到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处后得到的重组质粒。(5)pCambia1300-CA1载体：将SEQIDNo.9和SEQIDNo.10所示的2个序列直接顺次连接后插入到pCambia1300载体的“GGCCAGTGCCAAGCTT”和“CCCGGGCTGCAAGTTCAA”处后得到的重组质粒。二、转基因材料的创制及鉴定1、将步骤一构建的重组质粒MGTV导入农杆菌AGL1，得到重组农杆菌。2、取步骤1得到的重组农杆菌，重悬于液体共培养培养基(YEP液体培养基+100mg/L乙酰丁香酮，pH5.2)，得到OD600nm＝0.14的菌液。3、取日本晴的胚性愈伤组织，用步骤2得到的菌液浸泡30min，然后经共培养、筛选、生根、壮苗，得到T0代植株。4、T0代植株自交获得T1代种子，T1代种子长成的植株即为T1代植株。5、用pCambia1300载体进行上述转化，得到转空载体植株。6、用pCambia1300-PPDK1、pCambia1300-PEPC1、pCambia1300-MDH6、pCambia1300-ME2和pCambia1300-CA1载体进行上述转化，得到5种转单基因植株。用表2中对应的引物序列扩增再生植株DNA，电泳确定阳性株系。从鉴定阳性的转多基因株系中随机选择4个，分别编号为OV-1、OV-2、OV-3和OV-4。三、光合参数测定取生长状态一致的日本晴、步骤二得到的4个转基因阳性系(OV-1、OV-2、OV-3和OV-4)、空载对照、转单个基因的T1代植株，在盛花期，利用LI-COR6400进行光合参数的测定，并统计表5中所示的其他农艺性状。结果表明，与空载对照、野生对照、转单个基因(PPDK1、PEPC1、MDH6、ME2、CA1)相比，4个阳性株系(OV-1、OV-2、OV-3和OV-4)均不同程度地大辐度提高光合效率，进而提高千粒重，且没有呈现不利的农艺性状表型。见图2和表5。表5转基因与空对照的各个农艺性状比较注：*表明与空对照相比，差异显著(P<0.05)。<110>中国农业科学院生物技术研究所<120>转C4光合关键基因提高C3植物光合作用的方法<130>GNCLN180019<160>15<170>PatentInversion3.5<210>1<211>2000<212>DNA<213>玉米（ZeamaysL.）<400>1gagattttggggttatggtcgcaagggttatgaatgaagacttaccaggtaaatgggttc60agaatattttgaaagtccgagaggatgatccatgctttgctttaatcaaggttaagtata120atactaaaggtaaattttgctctcaattttggaagggaatactatatgcaaggaacttgg180taaatggggattatcaacaacgattaacaatgagaaaatgtactattttgggaggaggta240tgggttttggacatgccactaatattagaattcccattttttgttgtttgtcaaaataat300cattgtttggtcagtggttgttaggaaggaggtggatagaaagttaaatttagattttcc360ctggggtggaggacatgaaagagtgggaaaggttgctggacattttggaaggagtgataa420ttactaaacgagaatatatgttatcttcgtcgttagagaaatctagacagtatacaacaa480gatccacgtactacaggtaaacttttaggggtattgtgaacaagaggatgagtaaactct540aaaagaacaaagctccaatgaaaatttaggtttttatgtggttagtcatagggcaagttg600caaacaggtgttgatctaaaaaggaagtagtagggaaatgtgaagtgtctttgcgaggaa660ttggaaaatgaagatcacattttctttgggtgcatcatgggaagaaccatttgggactct720tttaaggaggcctaagaatgccataaagtttgcaagatctttttgaagagtgtctaccta780taaacaatagtaaatatcatgtcaaaattttcatcttcgccattattctttaggagaatt840tagaatgttccgaataaaatatggatagaaaagaagttcccaaagtcatccaattttcta900caaaatcttcaactttaagattgagagtgggtgttgtaaagttcttggaagatgagttga960accccatggaggcgttggctaaagtactgaaagcaatctaaagacatggaggtggaaggc1020ctgacgtagatagagaagatgctcttagctttcattgtctttcttttgtagtcatctgat1080ttacctctctcgtttatacaactggttttttaaacactccttaacttttcaaattgtctc1140tttctttaccctagactagataattttaatggtgattttgctaatgtggcgccatgttag1200atagaggtaaaatgaactagttaaaagctcagagtgataaatcaggctctcaaaaattca1260taaactgttttttaaatatccaaatatttttacatggaaaataataaaatttagtttagt1320attaaaaaattcagttgaatatagttttgtcttcaaaaattatgaaactgatcttaatta1380tttttccttaaaaccgtgctctatctttgatgtctagtttgagacgattatataattttt1440tttgtgcttaactacgacgagctgaagtacgtagaaatactagtggagtcgtgccgcgtg1500tgcctgtagccactcgtacgctacagcccaagcgctagagcccaagaggccggaggtgga1560aggcgtcgcggcactatagccactcgccgcaagagcccaagaggccggagctggaaggat1620gagggtctgggtgttcacgaattgcctggaggcaggaggctcgtcgtccggagccacagg1680cgtggagacgtccgggataaggtgagcagccgctgcgataggggcgcgtgtgaaccccgt1740cgcgccccacggatggtataagaataaaggcattccgcgtgcaggattcacccgttcgcc1800tctcaccttttcgctgtactcactcgccacacacaccccctctccagctccgttggagct1860ccggacagcagcaggcgcggggcggtcacgtagtaagcagctctcggctccctctcccct1920tgctccatatgatcgtgcaacccatcgagctacgcgcgtggactgccttccctgggtcgg1980cgcaggaggggatcggaagg2000<210>2<211>2844<212>DNA<213>玉米（ZeamaysL.）<400>2atggcggcatcggtttccagggccatctgcgtacagaagccgggctcaaaatgcaccagg60gacagggaagcgacctccttcgcccgccgatcggtcgcagcgccgaggcccccgcacgcc120aaagcccgccggcgtcatccgctccgactccggcgcgggacggggccacattgctcgccg180ctgagggccgtcgttgacgccgcgccgatacagacgaccaaaaagagggtgttccacttc240ggcaagggcaagagcgagggcaacaagaccatgaaggaactgctgggcggcaagggcgcg300aacctggcggagatggcgagcatcgggctgtcggtgccgccagggttcacggtgtcgacg360gaggcgtgccagcagtaccaggacgccgggtgcgccctccccgcggggctctgggccgag420atcgtcgacggcctgcagtgggtggaggagtacatgggcgccaccctgggcgatccgcag480cgcccgctcctgctctccgtccgctccggcgccgccgtgtccatgcccggcatgatggac540acggtgctcaacctggggctcaacgacgaagtggccgccgggctggcggccaagagcggg600gagcgcttcgcctacgactccttccgccgcttcctcgacatgttcggcaacgtcgtcatg660gacatcccccgctcactgttcgaagagaagcttgagcacatgaaggaatccaaggggctg720aagaacgacaccgacctcacggcctctgacctcaaagagctcgtgggtcagtacaaggag780gtctacctctcagccaagggagagccattcccctcagaccccaagaagcagctggagcta840gcagtgctggctgtgttcaactcgtgggagagccccagggccaagaagtacaggagcatc900aaccagatcactggcctcaggggcaccgccgtgaacgtgcagtgcatggtgttcggcaac960atggggaacacttctggcaccggcgtgctcttcaccaggaaccccaacaccggagagaag1020aagctgtatggcgagttcctggtgaacgctcagggtgaggatgtggttgccggaataaga1080accccagaggaccttgacgccatgaagaacctcatgccacaggcctacgacgagcttgtt1140gagaactgcaacatcctggagagccactacaaggaaatgcaggatatcgagttcactgtc1200caggaaaacaggctgtggatgttgcagtgcaggacagggaaacgtacgggcaaaagtgcc1260gtgaagatcgccgtggacatggttaacgagggccttgttgagccccgctcagcgatcaag1320atggtagagccaggccacctggaccagcttcttcatcctcagtttgagaacccgtcggcg1380tacaaggatcaagtcattgccactggtctgccagcctcacctggggctgctgtgggccag1440gttgtgttcactgctgaagatgctgaagcatggcattcccaagggaaagctgctattctg1500gtaagggcggagaccagccctgaggacgttggtggcatgcacgctgctgtggggattctt1560acagagaggggtggcatgacttcccacgctgctgtggtcgcacgttggtgggggaaatgc1620tgcgtctcgggatgctcaggcattcgcgtaaacgatgcggagaagctcgtgacgatcgga1680agccatgtgctgcgcgaaggtgagtggctgtcgctgaatgggtcgactggtgaggtgatc1740cttgggaagcagccgctttccccaccagcccttagtggtgatctgggaactttcatggcc1800tgggtggatgatgttagaaagctcaaggtcctggctaacgccgatacccctgatgatgca1860ttgactgcgcgaaacaatggggcacaaggaattggattatgccggacagagcacatgttc1920tttgcttcagacgagaggattaaggctgtcaggcagatgattatggctcccacgcttgag1980ctgaggcagcaggcgctcgaccgtctcttgacgtatcagaggtctgacttcgaaggcatt2040ttccgtgctatggatggactcccggtgaccatccgactcctggaccatccttcttacgag2100ttccttccagaagggaacatcgaggacattgtaagtgaattatgtgctgagacgggagcc2160aaccaggaggatgccctcgcgcgaattgaaaagctttcagaagtaaacccgatgcttggc2220ttccgtgggtgcaggcttggtatatcgtaccctgaattgacagagatgcaagcccgggcc2280atttttgaagctgctatagcaatgaccaaccagggtgttcaagtgttcccagagataatg2340gttcctcttgttggaacaccacaggaactggggcatcaagtgactcttatccgccaagtt2400gctgagaaagtgttcgccaatgtgggcaagactatcgggtacaaagttggaacaatgatt2460gagatccccagggcagctctggtggctgatgagatagcggagcaggctgaattcttctcc2520ttcggaacgaacgacctgacgcagatgacctttgggtacagcagggatgatgtgggaaag2580ttcattcccgttcatcttgctcagggcatcctccaacatgaccccttcgaggtcctggac2640cagaggggagtgggcgagctggtgaagtttgctacagagaggggccgcaaagctaggcct2700aacttgaaggtgggcatttgtggagaacacggtggagagccttcgtctgtggccttcttc2760gcgaaggctgggctggatttcgtttcttgctcccctttcagggttccgattgctaggcta2820gctgcagctcaggtgcttgtctga2844<210>3<211>1991<212>DNA<213>玉米（ZeamaysL.）<400>3tctcttaatcagtatatataattaaaaaactaatttcacatttgtgttcctaatattttt60tacaaataaatcattgtttaattccatttgtaataagtttttattaaaattgcttttatt120tcatttattataaacatttaattgttttaatcctattttagttttaatttattgtatcta180tttattaatataacgaacttcgataagaaacaaaagcaaggtcaaggtgttttttcaaag240tagttgtggaaaagctgaaccccttttattcaacttttagaagcaggaaaacagaaccaa300acagaccctaaaaatgtgtgaatttttagcaggttaattattcgcatctctttggtcatg360tttaagaggctggaatagatcaactgcaagaacacatagcagagtggatagggggggggg420gggggggggggggggagggtcgtcgtctccctatctgacctctcttctgcattggattgc480ctttttcggtactctatttaaaacttaaaagtacaaatgaggtgccggattgatggagtg540atatataagtttgatgtgtttttcacataagtgacaagtattattgaaagagaacatttg600cattgctactgtttgcatatgggaaaattgagaattgtatcatgccatggccgatcagtt660ctttacttagctcgatgtaatgcacaatgttgatagtatgtcgaggatctagcgatgtaa720tggtgttaggacacgtggttagctactaatataaatgtaaggtcattcgatggtttttct780attttcaattacctagcattatctcatttctaattgtgataacaaatgcattagaccata840attctgtaaatatgtacatttaagcacacagtctatattttaaaattcttctttttgtgt900ggatatcccaacccaaatccacctctctcttcaatccgtgcatgttcaccgctgccaagt960gccaacaacacatcgcatcgtgcatatctttgttggcttgtgcacggtcggcgccaatgg1020aggagacacctgtacggtgcccttggtagaacaacatccttatccctatatgtatggtgc1080ccttcgtagaatgacaccccttatccctacaatagccatgtatgcataccaagaattaaa1140tatactttttcttgaaccacaataatttattatagcggcacttcttgttcaggttgaaca1200cttatttggaacaataaaatgccgagttcctaaccacaggttcacttttttttttcctta1260tcctcctaggaaactaaattttaaaatcataaatttaatttaaatgttaatggaaacaaa1320aaattatctacaaagacgactcttagccacagccgcctcactgcaccctcaaccacatcc1380tgcaaacagacaccctcgccacatccctccagattcttcactccgatgcagcctacttgc1440taacagacgccctctccacatcctgcaaagcattcctccaaattcttgcgatcccccgaa1500tccagcattaactgctaagggacgccctctccacatcctgctacccaattagccaacgga1560ataacacaagaaggcaggtgagcagtgacaaagcacgtcaacagcaccgagccaagccaa1620aaaggagcaaggaggagcaagcccaagccgcagccgcagctctccaggtccccttgcgat1680tgccgccagcagtagcagacacccctctccacatcccctccggccgctaacagcagcaag1740ccaagccaaaaaggagcctcagccgcagccggttccgttgcggttaccgccgatcacatg1800cccaaggccgcgcctttccgaacgccgagggccgcccgttcccgtgcacagccacacaca1860cacccgcccgccaacgactccccatccctatttgaacccacccgcgcactgcattgatca1920ccaatcgcatcgcagcagcacgagcagcacgccgtgccgctccaaccatctcgcttccgt1980gcttagcttcc1991<210>4<211>2913<212>DNA<213>玉米（ZeamaysL.）<400>4atggcgtcgaccaaggcacccggccctggcgagaagcaccactccatcgacgcgcagctc60cgtcagctggtcccaggcaaggtctccgaggacgacaagctcatcgagtacgatgcgctg120ctcgtcgaccgcttcctcaacatcctccaggacctccacgggcccagccttcgcgaattt180gtccaggagtgctacgaggtgtcggccgactatgagggcaaaggagacacgacgaagctg240ggcgagctcggcgccaagctcacggggctggcccccgccgacgccatcctcgtggcgagc300tccatcctgcacatgctcaacctcgccaacctggccgaggaggtgcagatcgcgcaccgc360cgccgcaacagcaagctcaagaaaggtgggttcgccgacgagggctccgccaccaccgag420tccgacatcgaggagacgctcaagcgcctcgtgtccgaggtcggcaagtcccccgaggag480gtgttcgaggcgctcaagaaccagaccgtcgacctcgtcttcaccgcgcatcccacgcag540tccgcccgccgctcgctcctgcagaaaaacgccaggatccggaattgtctgacccagctg600aatgccaaggacatcactgacgacgacaagcaggagctcgatgaggctctgcagagagag660atccaagcagccttcagaaccgatgaaatcaggagggcacaacccaccccccaggacgaa720atgcgctatgggatgagctacatccatgagactgtatggaagggcgtgcctaagttcttg780cgccgtgtggatacagccctgaagaatatcggcatcaatgagcgccttccctacaatgtt840tctctcattcggttctcttcttggatgggtggtgaccgcgatggaaatccaagagttacc900ccggaggtgacaagagatgtatgcttgctggccagaatgatggctgcaaacttgtacatc960gatcagattgaagagctgatgtttgagctctctatgtggcgctgcaacgatgagcttcgt1020gttcgtgccgaagagctccacagttcgtctggttccaaagttaccaagtattacatagaa1080ttctggaagcaaattcctccaaacgagccctaccgggtgatactaggccatgtaagggac1140aagctgtacaacacacgcgagcgtgctcgccatctgctggcttctggagtttctgaaatt1200tcagcggaatcgtcatttaccagtatcgaagagttccttgagccacttgagctgtgctac1260aaatcaccgtgtgactgcggcgacaaggccatcgcggacgggagcctcctggacctcctg1320cgccaggttttcacgttcgggctctccctggtgaagctggacatccggcaggagtcggag1380cggcacaccgacgtgatcgacgccatcaccacgcacctcggcatcgggtcgtaccgcgag1440tggtccgaggacaagcggcaggagtggctgctgtcggagctgcgaggcaagcgcccgctg1500ctgcccccggaccttccccagaccgaggagatcgccgacgtcatcggcgcgttccacgtc1560ctcgcggagctcccgcccgacagcttcggcccctacatcatctccatggcgacggccccc1620tcggacgtgctcgccgtggagctcctgcagcgcgagtgcggcgtgcgccagccgctgccc1680gtggtgccgctgttcgaaaggctggccgacctgcagtcggcgcccgcgtccgtggagcgc1740ctcttctcggtggactggtacatggaccggatcaagggcaagcagcaggtcatggtcggc1800tactccgactccggcaaggacgccggccgcctgtccgcggcgtggcagctgtacagggcg1860caggaggagatggcgcaggtggccaagcgctacggcgtcaagctcaccttgttccacggc1920cgcggaggcaccgtgggcaggggtggcgggcccacgcaccttgccatcctgtcccagccg1980ccggacaccatcaacgggtccatccgtgtgacggtgcagggcgaggtcatcgagttctgc2040ttcggggaggaacacctgtgcttccagactctgcagcgcttcacggccgccacgctggag2100cacggcatgcacccgccggtctctcccaagtccgagtggcgcaagctcatggacgagatg2160gcggtcgtggccacggaggagtaccgctccgtcgtcgtcaaggaggcgcgcttcgtcgag2220tacttcagatcggctacaccggagaccgagtacgggaggatgaacatcggcagccggcca2280gccaagaggaggcccggcggcggcatcacgaccctgcgcgccatcccctggatcttctcg2340tggacccagaccaggttccacccccccgtgtggctgggagtcggcgccgcattcaagttc2400gccatcgacaaggacgtcaggaacttccaggtcctcaaagagatgtacaacgagtggcca2460ttcttcagggtcaccctggacctgctggagatggttttcgccaagggagaccccggcatt2520gccggcttgtatgacgagctgcttgtggcggaagaactcaagccctttgggaagcagctc2580agggacaaatacgtggagacacagcagcttctcctccagatcgctgggcacaaggatatt2640cttgaaggcgatccattcctgaagcaggggctggtgctgcgcaacccctacatcaccacc2700ctgaacgtgttccaggcctacacgctgaagcggataagggaccccaacttcaaggtgacg2760ccccagccgccgctgtccaaggagttcgccgacgagaacaagcccgccggactggtcaag2820ctgaacccggcgagcgagtacccgcccggcctggaagacacgctcatcctcaccatgaag2880ggcatcgccgccggcatgcagaacactggctag2913<210>5<211>538<212>DNA<213>人工序列<220><223><400>5catggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaaca60gttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtgga120gcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggc180aattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagc240tatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatca300ttgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatgg360acccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagca420agtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc480gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggact538<210>6<211>1299<212>DNA<213>玉米（ZeamaysL.）<400>6atgggcctttcaacagtttactccccggccggaccgcgcctcgtgccggcccctctcggc60cgctgccgctctgcccagccccgccgcccgcgccgagccccgctcgccaccgtccggtgc120tccgtcgacgccaccaagcaggcgcaggatggcgtggcgacggcggtcgcgacggaggcg180ccggcgtcgcggaaggagtgcttcggggtcttctgcaccacctacgacctcaaggcggag240gacaagaccaagtcgtggaggaagctagtgaatgttgctgtgtcaggcgcggccgggatg300atatcaaatcacctgctgttcaaactcgcttctggtgaggttttcggacaagaccaacca360atagcacttaagctactcggctcagaaagatcgtttcaagccctcgaaggtgtagctatg420gaactggaggactcgctgtatccactgctgagggaagtcagcattggcatagatccttac480gtggtctttcaagatgtagattgggcccttcttattggcgctaagccccgaggtcccggc540atggagcgagctgcgctactggatatcaatggccaaatctttgctgaccaggggaaagca600cttaatgcggtcgcctcgcggaacgacgaagtcttagttgtcggaaatccctgtaacact660aatgcgctgatttgtttgaaaaatgccccaaacataccggcaaaaaactttcatgcattg720acgaggttggatgaaaatagagcaaagtgccagctggcactaaaagcaggtgtattttat780gacaaagtatcaaacgtgactatttgggggaaccattcgacgactcaggttcctgatttc840ttgaatgccaaaattgatggaagaccagtgaaagaagtcattaaggataccaagtggtta900gaggaagagttcaccctcacagttcaaaagcgtggaggtgtgctcatccaaaaatggggc960agatcatcagctgcatcgaccgctgtttcgatagtggatgctattagatccctcgtaact1020cctaccccagaaggtgactggttttctacaggggtttatacgactggaaatccttatggc1080atagcagaggatatcgtgttcagcatgccatgcagatcgaagggtgatggtgattacgaa1140ctagctagtgatgtgttgatggacgattttctctgggaacggattaaaaagagcgaagct1200gaattgcttgctgagaagaaatgcgttgcccatcttacaggagaggggaatgcattctgt1260gatcttccggaagataccatgctaccaggagaagtgtag1299<210>7<211>1993<212>DNA<213>人工序列<220><223><400>7ctgcagtgcagcgtgacccggtcgtgcccctctctagagataatgagcattgcatgtcta60agttataaaaaattaccacatattttttttgtcacacttgtttgaagtgcagtttatcta120tctttatacatatatttaaactttactctacgaataatataatctatagtactacaataa180tatcagtgttttagagaatcatataaatgaacagttagacatggtctaaaggacaattga240gtattttgacaacaggactctacagttttatctttttagtgtgcatgtgttctccttttt300ttttgcaaatagcttcacctatataatacttcatccattttattagtacatccatttagg360gtttagggttaatggtttttatagactaatttttttagtacatctattttattctatttt420agcctctaaattaagaaaactaaaactctattttagtttttttatttaataatttagata480taaaatagaataaaataaagtgactaaaaattaaacaaataccctttaagaaattaaaaa540aactaaggaaacatttttcttgtttcgagtagataatgccagcctgttaaacgccgtcga600cgagtctaacggacaccaaccagcgaaccagcagcgtcgcgtcgggccaagcgaagcaga660cggcacggcatctctgtcgctgcctctggacccctctcgagagttccgctccaccgttgg720acttgctccgctgtcggcatccagaaattgcgtggcggagcggcagacgtgagccggcac780ggcaggcggcctcctcctcctctcacggcaccggcagctacgggggattcctttcccacc840gctccttcgctttcccttcctcgcccgccgtaataaatagacaccccctccacaccctct900ttccccaacctcgtgttgttcggagcgcacacacacacaaccagatctcccccaaatcca960cccgtcggcacctccgcttcaaggtacgccgctcgtcctccccccccccccctctctacc1020ttctctagatcggcgttccggtccatggttagggcccggtagttctacttctgttcatgt1080ttgtgttagatccgtgtttgtgttagatccgtgctgctagcgttcgtacacggatgcgac1140ctgtacgtcagacacgttctgattgctaacttgccagtgtttctctttggggaatcctgg1200gatggctctagccgttccgcagacgggatcgatttcatgattttttttgtttcgttgcat1260agggtttggtttgcccttttcctttatttcaatatatgccgtgcacttgtttgtcgggtc1320atcttttcatgcttttttttgtcttggttgtgatgatgtggtctggttgggcggtcgttc1380tagatcggagtagaaatctgtttcaaactacctggtggatttattaattttggatctgta1440tgtgtgtgccatacatattcatagttacgaattgaagatgatggatggaaatatcgatct1500aggataggtatacatgttgatgcgggttttactgatgcatatacagagatgctttttgtt1560cgcttggttgtgatgatgtggtgtggttgggcggtcgttcattcgttctagatcggagta1620gaatactgtttcaaactacctggtgtatttattaattttggaactgtatgtgtgtgtcat1680acatcttcatagttacgagtttaagatggatggaaatatcgatctaggataggtatacat1740gttgatgtgggttttactgatgcatatacatgatggcatatgcagcatctattcatatgc1800tctaaccttgagtacctatctattataataaacaagtatgttttataattattttgatct1860tgatatacttggatgatggcatatgcagcagctatatgtggatttttttagccctgcctt1920catacgctatttatttgcttggtactgtttcttttgtcgatgctcaccctgttgtttggt1980gttacttctgcag1993<210>8<211>1989<212>DNA<213>玉米（ZeamaysL.）<400>8atgatctccgcgcgcaccgccgcagcttcccccgcttccctgtggaagcagggaggacga60agcgagggcggcggcagctgcgacggatgtcggacttacaggaacactttgcggagggcc120gcagcagcgaaggtgcgtgccttgccgtcgagggcggtggaggcggtcaaaatgggctcc180gccgcggagatggagcaggaggaggcggaggtggccgcggccgcggccggcgtcgtcgtc240gaggaccactacggcgaggacagcgccaccgaggagctgcccatcctgccctgggccttc300tccgtcgcaagatatgtttccttcagttggttgaaaaaatacgctgaggagtcttatttc360tacatcagcggttacaccctcttgagggatccacatcacaacaagggtcttgctttcatg420gagaaggagagggatgcacaccacttgcgggggctgcttcctcctgcagttgtgcctcaa480gaactccaaattaagaagatcatgcacaacctgcggcagtaccaggtccctttgcagcgc540tatatggccatgatggaccttcaggagaggaacgagaggcttttctacaagcttttaatt600gataacgtggaggagctgcttcctgtggtttatacaccaactgtaggtgaggcctgccag660aagtatgggtccatctttcgacaaccacagggtctgtatgtcagcttgagggacaagggg720aaggtcctagaagttctaaggaactggccacataggaacattcaagttatctgtgttact780gatggtgaggcaatcttgggacttggagatttgggttctcagggaatgggaattcctgta840ggcaaacttgctctatacactgctcttggaggagttcgtccatcagcttgtttgcctatc900acaatcgatgttggcacaaataatgagaaactgcttaatgatgagttttacattggactc960cggcaaaaacgtgcaactggcgaggagtatcatgagcttatggaagagttcatggctgct1020gttaagcaaatctacggcgagaaagtcctcattcagtttgaggacttcgcgaatcataat1080gcttttaatttgcttgcaaaatatagcaagagccatcttgttttcaatgatgacatccag1140ggcacagcatcagtggtccttgcaggtttgttagcagcactcaaggtggttggtaggacc1200ttggcagagcacacttatttattccttggtgctggggaggctggaactggtattgcagaa1260ctcattgctcttgagatttcaaaacagacgtcggctccaattgaagagtgccgcaagaag1320atttggttggtggactcaaagggtttgattgttgactctcgtaaaaactccgttcagtca1380ttcaaaaaaccttgggcacatgagcatgagcccttgacaaccttgtacgatgctgttcag1440tccatcaaacctacagttctgattgggacgtctggagttggaagaacattcacaaaagaa1500gttgttgaggccatggcttccttcaatgagaggcctatcatcttttcactgtcaaatcca1560acctctcattctgaatgtactgctgaagaagcatataactggactcaggtcgtaatattt1620gccagtggcagtccatttgaccctgtggagtacgatgggaaggtttttgtacctggacag1680tcgaacaacgcctacattttccctggatttggcctcggtcttgttatctctggagccatc1740cgtgtccacgaggacatgcttcttgccgcctcggaagcactagctgctcaggccacacag1800gagaacttcgacaagggatcaatcttcccacccttcaccaacattagaaagatctctgcg1860cgcattgccagagccgtggctgcaaaagcttatgaactcggtttggcgacccgtctgcct1920cccccaagggacctggtgaaatatgcacagagctgcatgtacactcctgtctaccgtaac1980taccggtag1989<210>9<211>1413<212>DNA<213>人工序列<220><223><400>9tagctagcatactcgaggtcattcatatgcttgagaagagagtcgggatagtccaaaata60aaacaaaggtaagattacctggtcaaaagtgaaaacatcagttaaaaggtggtataaagt120aaaatatcggtaataaaaggtggcccaaagtgaaatttactcttttctactattataaaa180attgaggatgtttttgtcggtactttgatacgtcatttttgtatgaattggtttttaagt240ttattcgcttttggaaatgcatatctgtatttgagtcgggttttaagttcgtttgctttt300gtaaatacagagggatttgtataagaaatatctttaaaaaaacccatatgctaatttgac360ataatttttgagaaaaatatatattcaggcgaattctcacaatgaacaataataagatta420aaatagctttcccccgttgcagcgcatgggtattttttctagtaaaaataaaagataaac480ttagactcaaaacatttacaaaaacaacccctaaagttcctaaagcccaaagtgctatcc540acgatccatagcaagcccagcccaacccaacccaacccaacccaccccagtccagccaac600tggacaatagtctccacacccccccactatcaccgtgagttgtccgcacgcaccgcacgt660ctcgcagccaaaaaaaaaaaaagaaagaaaaaaaagaaaaagaaaaaacagcaggtgggt720ccgggtcgtgggggccggaaacgcgaggaggatcgcgagccagcgacgaggccggccctc780cctccgcttccaaagaaacgccccccatcgccactatatacatacccccccctctcctcc840catccccccaaccctaccaccaccaccaccaccacctccacctcctcccccctcgctgcc900ggacgacgagctcctcccccctccccctccgccgccgccgcgccggtaaccaccccgccc960ctctcctctttctttctccgtttttttttccgtctcggtctcgatctttggccttggtag1020tttgggtgggcgagaggcggcttcgtgcgcgcccagatcggtgcgcgggaggggcgggat1080ctcgcggctggggctctcgccggcgtggatccggcccggatctcgcggggaatggggctc1140tcggatgtagatctgcgatccgccgttgttgggggagatgatggggggtttaaaatttcc1200gccatgctaaacaagatcaggaagaggggaaaagggcactatggtttatatttttatata1260tttctgctgcttcgtcaggcttagatgtgctagatctttctttcttctttttgtgggtag1320aatttgaatccctcagcattgttcatcggtagtttttcttttcatgatttgtgacaaatg1380cagcctcgtgcggagcttttttgtaggtagaag1413<210>10<211>1371<212>DNA<213>玉米（ZeamaysL.）<400>10atgtacacattgcccgtccgcgccaccacatccagcatcgtcgccagcctcgccaccccc60gcgccgtcctcctcctccggctccggctccggccgccccaggcccaggctcatccggaac120gcccccgtcttcgccgcccccgccaccgtcgtgggcatggaccccaccgtcgagcgcttg180aagagcgggttccagaagttcaagaccgaggtctatgacaagaagccggagctgttcgag240cctctcaagtccggccagagccccaggtacatggtgttcgcctgctccgactcccgcgtg300tgcccgtcggtgacactgggcctgcagcccggcgaggcattcaccgtccgcaacatcgcc360tccatggtcccaccctacgacaagatcaagtacgccggcaccgggtccgccatcgagtac420gccgtgtgcgcgctcaaggtgcaggtcatcgtggtcattggccacagctgctgcggtggc480atcagggcgctcctctccctcaaggacggcgcgcccgacaacttccacttcgtggaggac540tgggtcaggatcggcagccctgccaagaacaaggtgaagaaagagcacgcatcggtgccg600ttcgatgaccagtgctccatcctggagaaggaggccgtgaacgtgtcgctccagaacctc660aagagctaccccttcgtcaaggaagggctggccggcgggacgctcaagctggttggcgcc720cactacgacttcgtcaaagggcagttcgtcacatgggagcctccccaggacgccatcgag780cgcttgacgagcgggttccagcagttcaaggtcaatgtctatgacaagaagccggagctt840ttcgggcctctcaagtccggccaggcccccaagtacatggtgttcgcttgctccgactcc900cgtgtgtgcccatcggtgaccctgggcctgcagcccggcgaggccttcaccgttcgcaac960atagccgccatggtcccaggctacgacaagaccaagtacaccggcatcgggtccgccatc1020gagtacgctgtgtgcgctctcaaggtggaggtcctcgtggtcattggccatagctgctgc1080ggtggcatcagggcgctcctctccctccaggacggcgcacctgacaccttccacttcgtc1140gaggactgggttaagatcggcttcattgccaagatgaaggtaaagaaagagcacgcctcg1200gtgccgttcgatgaccagtgctccattctcgagaaggaggccgtgaacgtgtccctggag1260aacctcaagacctaccccttcgtcaaggaagggcttgcaaatgggaccctcaagctgatc1320ggcgcccactacgactttgtctcaggagagttcctcacatggaaaaagtga1371<210>11<211>947<212>PRT<213>玉米（ZeamaysL.）<400>11MetAlaAlaSerValSerArgAlaIleCysValGlnLysProGlySer151015LysCysThrArgAspArgGluAlaThrSerPheAlaArgArgSerVal202530AlaAlaProArgProProHisAlaLysAlaArgArgArgHisProLeu354045ArgLeuArgArgGlyThrGlyProHisCysSerProLeuArgAlaVal505560ValAspAlaAlaProIleGlnThrThrLysLysArgValPheHisPhe65707580GlyLysGlyLysSerGluGlyAsnLysThrMetLysGluLeuLeuGly859095GlyLysGlyAlaAsnLeuAlaGluMetAlaSerIleGlyLeuSerVal100105110ProProGlyPheThrValSerThrGluAlaCysGlnGlnTyrGlnAsp115120125AlaGlyCysAlaLeuProAlaGlyLeuTrpAlaGluIleValAspGly130135140LeuGlnTrpValGluGluTyrMetGlyAlaThrLeuGlyAspProGln145150155160ArgProLeuLeuLeuSerValArgSerGlyAlaAlaValSerMetPro165170175GlyMetMetAspThrValLeuAsnLeuGlyLeuAsnAspGluValAla180185190AlaGlyLeuAlaAlaLysSerGlyGluArgPheAlaTyrAspSerPhe195200205ArgArgPheLeuAspMetPheGlyAsnValValMetAspIleProArg210215220SerLeuPheGluGluLysLeuGluHisMetLysGluSerLysGlyLeu225230235240LysAsnAspThrAspLeuThrAlaSerAspLeuLysGluLeuValGly245250255GlnTyrLysGluValTyrLeuSerAlaLysGlyGluProPheProSer260265270AspProLysLysGlnLeuGluLeuAlaValLeuAlaValPheAsnSer275280285TrpGluSerProArgAlaLysLysTyrArgSerIleAsnGlnIleThr290295300GlyLeuArgGlyThrAlaValAsnValGlnCysMetValPheGlyAsn305310315320MetGlyAsnThrSerGlyThrGlyValLeuPheThrArgAsnProAsn325330335ThrGlyGluLysLysLeuTyrGlyGluPheLeuValAsnAlaGlnGly340345350GluAspValValAlaGlyIleArgThrProGluAspLeuAspAlaMet355360365LysAsnLeuMetProGlnAlaTyrAspGluLeuValGluAsnCysAsn370375380IleLeuGluSerHisTyrLysGluMetGlnAspIleGluPheThrVal385390395400GlnGluAsnArgLeuTrpMetLeuGlnCysArgThrGlyLysArgThr405410415GlyLysSerAlaValLysIleAlaValAspMetValAsnGluGlyLeu420425430ValGluProArgSerAlaIleLysMetValGluProGlyHisLeuAsp435440445GlnLeuLeuHisProGlnPheGluAsnProSerAlaTyrLysAspGln450455460ValIleAlaThrGlyLeuProAlaSerProGlyAlaAlaValGlyGln465470475480ValValPheThrAlaGluAspAlaGluAlaTrpHisSerGlnGlyLys485490495AlaAlaIleLeuValArgAlaGluThrSerProGluAspValGlyGly500505510MetHisAlaAlaValGlyIleLeuThrGluArgGlyGlyMetThrSer515520525HisAlaAlaValValAlaArgTrpTrpGlyLysCysCysValSerGly530535540CysSerGlyIleArgValAsnAspAlaGluLysLeuValThrIleGly545550555560SerHisValLeuArgGluGlyGluTrpLeuSerLeuAsnGlySerThr565570575GlyGluValIleLeuGlyLysGlnProLeuSerProProAlaLeuSer580585590GlyAspLeuGlyThrPheMetAlaTrpValAspAspValArgLysLeu595600605LysValLeuAlaAsnAlaAspThrProAspAspAlaLeuThrAlaArg610615620AsnAsnGlyAlaGlnGlyIleGlyLeuCysArgThrGluHisMetPhe625630635640PheAlaSerAspGluArgIleLysAlaValArgGlnMetIleMetAla645650655ProThrLeuGluLeuArgGlnGlnAlaLeuAspArgLeuLeuThrTyr660665670GlnArgSerAspPheGluGlyIlePheArgAlaMetAspGlyLeuPro675680685ValThrIleArgLeuLeuAspHisProSerTyrGluPheLeuProGlu690695700GlyAsnIleGluAspIleValSerGluLeuCysAlaGluThrGlyAla705710715720AsnGlnGluAspAlaLeuAlaArgIleGluLysLeuSerGluValAsn725730735ProMetLeuGlyPheArgGlyCysArgLeuGlyIleSerTyrProGlu740745750LeuThrGluMetGlnAlaArgAlaIlePheGluAlaAlaIleAlaMet755760765ThrAsnGlnGlyValGlnValPheProGluIleMetValProLeuVal770775780GlyThrProGlnGluLeuGlyHisGlnValThrLeuIleArgGlnVal785790795800AlaGluLysValPheAlaAsnValGlyLysThrIleGlyTyrLysVal805810815GlyThrMetIleGluIleProArgAlaAlaLeuValAlaAspGluIle820825830AlaGluGlnAlaGluPhePheSerPheGlyThrAsnAspLeuThrGln835840845MetThrPheGlyTyrSerArgAspAspValGlyLysPheIleProVal850855860HisLeuAlaGlnGlyIleLeuGlnHisAspProPheGluValLeuAsp865870875880GlnArgGlyValGlyGluLeuValLysPheAlaThrGluArgGlyArg885890895LysAlaArgProAsnLeuLysValGlyIleCysGlyGluHisGlyGly900905910GluProSerSerValAlaPhePheAlaLysAlaGlyLeuAspPheVal915920925SerCysSerProPheArgValProIleAlaArgLeuAlaAlaAlaGln930935940ValLeuVal945<210>12<211>970<212>PRT<213>玉米（ZeamaysL.）<400>12MetAlaSerThrLysAlaProGlyProGlyGluLysHisHisSerIle151015AspAlaGlnLeuArgGlnLeuValProGlyLysValSerGluAspAsp202530LysLeuIleGluTyrAspAlaLeuLeuValAspArgPheLeuAsnIle354045LeuGlnAspLeuHisGlyProSerLeuArgGluPheValGlnGluCys505560TyrGluValSerAlaAspTyrGluGlyLysGlyAspThrThrLysLeu65707580GlyGluLeuGlyAlaLysLeuThrGlyLeuAlaProAlaAspAlaIle859095LeuValAlaSerSerIleLeuHisMetLeuAsnLeuAlaAsnLeuAla100105110GluGluValGlnIleAlaHisArgArgArgAsnSerLysLeuLysLys115120125GlyGlyPheAlaAspGluGlySerAlaThrThrGluSerAspIleGlu130135140GluThrLeuLysArgLeuValSerGluValGlyLysSerProGluGlu145150155160ValPheGluAlaLeuLysAsnGlnThrValAspLeuValPheThrAla165170175HisProThrGlnSerAlaArgArgSerLeuLeuGlnLysAsnAlaArg180185190IleArgAsnCysLeuThrGlnLeuAsnAlaLysAspIleThrAspAsp195200205AspLysGlnGluLeuAspGluAlaLeuGlnArgGluIleGlnAlaAla210215220PheArgThrAspGluIleArgArgAlaGlnProThrProGlnAspGlu225230235240MetArgTyrGlyMetSerTyrIleHisGluThrValTrpLysGlyVal245250255ProLysPheLeuArgArgValAspThrAlaLeuLysAsnIleGlyIle260265270AsnGluArgLeuProTyrAsnValSerLeuIleArgPheSerSerTrp275280285MetGlyGlyAspArgAspGlyAsnProArgValThrProGluValThr290295300ArgAspValCysLeuLeuAlaArgMetMetAlaAlaAsnLeuTyrIle305310315320AspGlnIleGluGluLeuMetPheGluLeuSerMetTrpArgCysAsn325330335AspGluLeuArgValArgAlaGluGluLeuHisSerSerSerGlySer340345350LysValThrLysTyrTyrIleGluPheTrpLysGlnIleProProAsn355360365GluProTyrArgValIleLeuGlyHisValArgAspLysLeuTyrAsn370375380ThrArgGluArgAlaArgHisLeuLeuAlaSerGlyValSerGluIle385390395400SerAlaGluSerSerPheThrSerIleGluGluPheLeuGluProLeu405410415GluLeuCysTyrLysSerProCysAspCysGlyAspLysAlaIleAla420425430AspGlySerLeuLeuAspLeuLeuArgGlnValPheThrPheGlyLeu435440445SerLeuValLysLeuAspIleArgGlnGluSerGluArgHisThrAsp450455460ValIleAspAlaIleThrThrHisLeuGlyIleGlySerTyrArgGlu465470475480TrpSerGluAspLysArgGlnGluTrpLeuLeuSerGluLeuArgGly485490495LysArgProLeuLeuProProAspLeuProGlnThrGluGluIleAla500505510AspValIleGlyAlaPheHisValLeuAlaGluLeuProProAspSer515520525PheGlyProTyrIleIleSerMetAlaThrAlaProSerAspValLeu530535540AlaValGluLeuLeuGlnArgGluCysGlyValArgGlnProLeuPro545550555560ValValProLeuPheGluArgLeuAlaAspLeuGlnSerAlaProAla565570575SerValGluArgLeuPheSerValAspTrpTyrMetAspArgIleLys580585590GlyLysGlnGlnValMetValGlyTyrSerAspSerGlyLysAspAla595600605GlyArgLeuSerAlaAlaTrpGlnLeuTyrArgAlaGlnGluGluMet610615620AlaGlnValAlaLysArgTyrGlyValLysLeuThrLeuPheHisGly625630635640ArgGlyGlyThrValGlyArgGlyGlyGlyProThrHisLeuAlaIle645650655LeuSerGlnProProAspThrIleAsnGlySerIleArgValThrVal660665670GlnGlyGluValIleGluPheCysPheGlyGluGluHisLeuCysPhe675680685GlnThrLeuGlnArgPheThrAlaAlaThrLeuGluHisGlyMetHis690695700ProProValSerProLysSerGluTrpArgLysLeuMetAspGluMet705710715720AlaValValAlaThrGluGluTyrArgSerValValValLysGluAla725730735ArgPheValGluTyrPheArgSerAlaThrProGluThrGluTyrGly740745750ArgMetAsnIleGlySerArgProAlaLysArgArgProGlyGlyGly755760765IleThrThrLeuArgAlaIleProTrpIlePheSerTrpThrGlnThr770775780ArgPheHisProProValTrpLeuGlyValGlyAlaAlaPheLysPhe785790795800AlaIleAspLysAspValArgAsnPheGlnValLeuLysGluMetTyr805810815AsnGluTrpProPhePheArgValThrLeuAspLeuLeuGluMetVal820825830PheAlaLysGlyAspProGlyIleAlaGlyLeuTyrAspGluLeuLeu835840845ValAlaGluGluLeuLysProPheGlyLysGlnLeuArgAspLysTyr850855860ValGluThrGlnGlnLeuLeuLeuGlnIleAlaGlyHisLysAspIle865870875880LeuGluGlyAspProPheLeuLysGlnGlyLeuValLeuArgAsnPro885890895TyrIleThrThrLeuAsnValPheGlnAlaTyrThrLeuLysArgIle900905910ArgAspProAsnPheLysValThrProGlnProProLeuSerLysGlu915920925PheAlaAspGluAsnLysProAlaGlyLeuValLysLeuAsnProAla930935940SerGluTyrProProGlyLeuGluAspThrLeuIleLeuThrMetLys945950955960GlyIleAlaAlaGlyMetGlnAsnThrGly965970<210>13<211>432<212>PRT<213>玉米（ZeamaysL.）<400>13MetGlyLeuSerThrValTyrSerProAlaGlyProArgLeuValPro151015AlaProLeuGlyArgCysArgSerAlaGlnProArgArgProArgArg202530AlaProLeuAlaThrValArgCysSerValAspAlaThrLysGlnAla354045GlnAspGlyValAlaThrAlaValAlaThrGluAlaProAlaSerArg505560LysGluCysPheGlyValPheCysThrThrTyrAspLeuLysAlaGlu65707580AspLysThrLysSerTrpArgLysLeuValAsnValAlaValSerGly859095AlaAlaGlyMetIleSerAsnHisLeuLeuPheLysLeuAlaSerGly100105110GluValPheGlyGlnAspGlnProIleAlaLeuLysLeuLeuGlySer115120125GluArgSerPheGlnAlaLeuGluGlyValAlaMetGluLeuGluAsp130135140SerLeuTyrProLeuLeuArgGluValSerIleGlyIleAspProTyr145150155160ValValPheGlnAspValAspTrpAlaLeuLeuIleGlyAlaLysPro165170175ArgGlyProGlyMetGluArgAlaAlaLeuLeuAspIleAsnGlyGln180185190IlePheAlaAspGlnGlyLysAlaLeuAsnAlaValAlaSerArgAsn195200205AspGluValLeuValValGlyAsnProCysAsnThrAsnAlaLeuIle210215220CysLeuLysAsnAlaProAsnIleProAlaLysAsnPheHisAlaLeu225230235240ThrArgLeuAspGluAsnArgAlaLysCysGlnLeuAlaLeuLysAla245250255GlyValPheTyrAspLysValSerAsnValThrIleTrpGlyAsnHis260265270SerThrThrGlnValProAspPheLeuAsnAlaLysIleAspGlyArg275280285ProValLysGluValIleLysAspThrLysTrpLeuGluGluGluPhe290295300ThrLeuThrValGlnLysArgGlyGlyValLeuIleGlnLysTrpGly305310315320ArgSerSerAlaAlaSerThrAlaValSerIleValAspAlaIleArg325330335SerLeuValThrProThrProGluGlyAspTrpPheSerThrGlyVal340345350TyrThrThrGlyAsnProTyrGlyIleAlaGluAspIleValPheSer355360365MetProCysArgSerLysGlyAspGlyAspTyrGluLeuAlaSerAsp370375380ValLeuMetAspAspPheLeuTrpGluArgIleLysLysSerGluAla385390395400GluLeuLeuAlaGluLysLysCysValAlaHisLeuThrGlyGluGly405410415AsnAlaPheCysAspLeuProGluAspThrMetLeuProGlyGluVal420425430<210>14<211>662<212>PRT<213>玉米（ZeamaysL.）<400>14MetIleSerAlaArgThrAlaAlaAlaSerProAlaSerLeuTrpLys151015GlnGlyGlyArgSerGluGlyGlyGlySerCysAspGlyCysArgThr202530TyrArgAsnThrLeuArgArgAlaAlaAlaAlaLysValArgAlaLeu354045ProSerArgAlaValGluAlaValLysMetGlySerAlaAlaGluMet505560GluGlnGluGluAlaGluValAlaAlaAlaAlaAlaGlyValValVal65707580GluAspHisTyrGlyGluAspSerAlaThrGluGluLeuProIleLeu859095ProTrpAlaPheSerValAlaArgTyrValSerPheSerTrpLeuLys100105110LysTyrAlaGluGluSerTyrPheTyrIleSerGlyTyrThrLeuLeu115120125ArgAspProHisHisAsnLysGlyLeuAlaPheMetGluLysGluArg130135140AspAlaHisHisLeuArgGlyLeuLeuProProAlaValValProGln145150155160GluLeuGlnIleLysLysIleMetHisAsnLeuArgGlnTyrGlnVal165170175ProLeuGlnArgTyrMetAlaMetMetAspLeuGlnGluArgAsnGlu180185190ArgLeuPheTyrLysLeuLeuIleAspAsnValGluGluLeuLeuPro195200205ValValTyrThrProThrValGlyGluAlaCysGlnLysTyrGlySer210215220IlePheArgGlnProGlnGlyLeuTyrValSerLeuArgAspLysGly225230235240LysValLeuGluValLeuArgAsnTrpProHisArgAsnIleGlnVal245250255IleCysValThrAspGlyGluAlaIleLeuGlyLeuGlyAspLeuGly260265270SerGlnGlyMetGlyIleProValGlyLysLeuAlaLeuTyrThrAla275280285LeuGlyGlyValArgProSerAlaCysLeuProIleThrIleAspVal290295300GlyThrAsnAsnGluLysLeuLeuAsnAspGluPheTyrIleGlyLeu305310315320ArgGlnLysArgAlaThrGlyGluGluTyrHisGluLeuMetGluGlu325330335PheMetAlaAlaValLysGlnIleTyrGlyGluLysValLeuIleGln340345350PheGluAspPheAlaAsnHisAsnAlaPheAsnLeuLeuAlaLysTyr355360365SerLysSerHisLeuValPheAsnAspAspIleGlnGlyThrAlaSer370375380ValValLeuAlaGlyLeuLeuAlaAlaLeuLysValValGlyArgThr385390395400LeuAlaGluHisThrTyrLeuPheLeuGlyAlaGlyGluAlaGlyThr405410415GlyIleAlaGluLeuIleAlaLeuGluIleSerLysGlnThrSerAla420425430ProIleGluGluCysArgLysLysIleTrpLeuValAspSerLysGly435440445LeuIleValAspSerArgLysAsnSerValGlnSerPheLysLysPro450455460TrpAlaHisGluHisGluProLeuThrThrLeuTyrAspAlaValGln465470475480SerIleLysProThrValLeuIleGlyThrSerGlyValGlyArgThr485490495PheThrLysGluValValGluAlaMetAlaSerPheAsnGluArgPro500505510IleIlePheSerLeuSerAsnProThrSerHisSerGluCysThrAla515520525GluGluAlaTyrAsnTrpThrGlnValValIlePheAlaSerGlySer530535540ProPheAspProValGluTyrAspGlyLysValPheValProGlyGln545550555560SerAsnAsnAlaTyrIlePheProGlyPheGlyLeuGlyLeuValIle565570575SerGlyAlaIleArgValHisGluAspMetLeuLeuAlaAlaSerGlu580585590AlaLeuAlaAlaGlnAlaThrGlnGluAsnPheAspLysGlySerIle595600605PheProProPheThrAsnIleArgLysIleSerAlaArgIleAlaArg610615620AlaValAlaAlaLysAlaTyrGluLeuGlyLeuAlaThrArgLeuPro625630635640ProProArgAspLeuValLysTyrAlaGlnSerCysMetTyrThrPro645650655ValTyrArgAsnTyrArg660<210>15<211>456<212>PRT<213>玉米（ZeamaysL.）<400>15MetTyrThrLeuProValArgAlaThrThrSerSerIleValAlaSer151015LeuAlaThrProAlaProSerSerSerSerGlySerGlySerGlyArg202530ProArgProArgLeuIleArgAsnAlaProValPheAlaAlaProAla354045ThrValValGlyMetAspProThrValGluArgLeuLysSerGlyPhe505560GlnLysPheLysThrGluValTyrAspLysLysProGluLeuPheGlu65707580ProLeuLysSerGlyGlnSerProArgTyrMetValPheAlaCysSer859095AspSerArgValCysProSerValThrLeuGlyLeuGlnProGlyGlu100105110AlaPheThrValArgAsnIleAlaSerMetValProProTyrAspLys115120125IleLysTyrAlaGlyThrGlySerAlaIleGluTyrAlaValCysAla130135140LeuLysValGlnValIleValValIleGlyHisSerCysCysGlyGly145150155160IleArgAlaLeuLeuSerLeuLysAspGlyAlaProAspAsnPheHis165170175PheValGluAspTrpValArgIleGlySerProAlaLysAsnLysVal180185190LysLysGluHisAlaSerValProPheAspAspGlnCysSerIleLeu195200205GluLysGluAlaValAsnValSerLeuGlnAsnLeuLysSerTyrPro210215220PheValLysGluGlyLeuAlaGlyGlyThrLeuLysLeuValGlyAla225230235240HisTyrAspPheValLysGlyGlnPheValThrTrpGluProProGln245250255AspAlaIleGluArgLeuThrSerGlyPheGlnGlnPheLysValAsn260265270ValTyrAspLysLysProGluLeuPheGlyProLeuLysSerGlyGln275280285AlaProLysTyrMetValPheAlaCysSerAspSerArgValCysPro290295300SerValThrLeuGlyLeuGlnProGlyGluAlaPheThrValArgAsn305310315320IleAlaAlaMetValProGlyTyrAspLysThrLysTyrThrGlyIle325330335GlySerAlaIleGluTyrAlaValCysAlaLeuLysValGluValLeu340345350ValValIleGlyHisSerCysCysGlyGlyIleArgAlaLeuLeuSer355360365LeuGlnAspGlyAlaProAspThrPheHisPheValGluAspTrpVal370375380LysIleGlyPheIleAlaLysMetLysValLysLysGluHisAlaSer385390395400ValProPheAspAspGlnCysSerIleLeuGluLysGluAlaValAsn405410415ValSerLeuGluAsnLeuLysThrTyrProPheValLysGluGlyLeu420425430AlaAsnGlyThrLeuLysLeuIleGlyAlaHisTyrAspPheValSer435440445GlyGluPheLeuThrTrpLysLys450455当前第1页1 2 3