一种具有优越检测线和分析灵敏度的尿素检测试剂及检测方法与流程

技术特征：

1.一种UREA检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：

1）试剂R1的组分为：

缓冲液_{··························································}100mmol/L

NADPH_{···························································}0.3mmol/L

2）试剂R2的组分为：

缓冲液_{··························································}100mmol/L

脲酶_{····························································}40KU/L

谷氨酸脱氢酶_{····················································}2KU/L

α-酮戊二酸_{······················································}30mmol/L

防腐剂_{··························································}0.8g/L_·。

2.根据权利要求1所述的UREA检测试剂，其特征在于试剂R1中缓冲液为25℃，pH为9.1的三羟甲基氨基甲烷缓冲液。

3.根据权利要求1所述的UREA检测试剂，其特征在于试剂R2中缓冲液为25℃，pH为7.6的三羟甲基氨基甲烷缓冲液。

4.根据权利要求1所述的UREA检测试剂，其特征在于所述防腐剂为NaN₃。

5.一种使用权利要求1-4中任一项所述检测UREA的检测方法，其特征在于使用全自动生化分析仪利用速率法进行测定，检测主波长为340nm。

6.根据权利要求5所述的检测方法，其特征在于R1试剂和R2试剂的比例为4:1。