产热的miRNA调节剂的制作方法
【专利说明】产热的miRNA调节剂
[誦] 目录
[0002] 相关申请的交叉引用.......................................................3
[0003] W胃技术.................................................................3
[0004] 发明容.................................................................3
[0005] 附卽兑明................................................................15
[0006]
【具体实施方式】............................................................18
[0007] I.定义..............................................................18
[0008] II.产热和服胖.........................................................22
[0009] III.miRNA试剂.........................................................24
[0010] IV.治疗方法...........................................................33
[0011] V.筛选方法............................................................38
[0012] VI.药物组合物.........................................................39
[0013] VII.例证..............................................................43
[0014] 实施例1.产热调节子的计算机分析......................................44
[0015] 实施例2.相关miRNA碑1标的计算机选择...................................48
[0016] A) -种微小RNA-多种mRNA通路特异性的范例............................52
[0017] A1. -种微小RNA-多种mRNA通路特异性的范例的第一个实例.............53
[0018] A2. -种微小RNA-多种mRNA通路特异性的范例的另一些实例.............55
[0019] B)多种microRNA- -种mRNA范例.......................................58
[0020] B1.包括UCP1的一个示例性的多种miRNA- -种mRNA范例.................58
[0021] B2.包括UCP2的另一个示例性的多种miRNA- -种mRNA范例...............77
[0022] C)多种微小RNA-多种mRNA范例........................................83
[0023] D)共调控的mRNA祀标中的一种微小RNA种子序列基序的过呈现范例.........83
[0024] 巧内含子miRNA-多种mRNA通路特异性的范例............................88
[00巧]实施例3.高内涵细胞表型筛选..........................................88
[0026] A.人前脂肪细胞分化成脂肪细胞......................................89
[0027] 1.分化方案......................................................89
[0028] 2.前脂肪细胞的转染..............................................90
[0029] 3.在人前脂肪细胞分化成脂肪细胞过程中的表型变化..................90
[0030] 4.在人前脂肪细胞分化成脂肪细胞过程中的基因型变化................90
[0031] B.人白色脂肪细胞分化成褐色脂肪细胞................................94
[0032] 1.分化方案......................................................94
[0033] 2.脂肪细胞的转染................................................95
[0034] 3.人脂肪细胞在培养30天的维持过程中表型的变化................................95
[00；35] 4.人成熟月旨肪细胞转染的优化..................................................96
[0036] 5.在miRNA类似物或已知的脂肪生成和/或产热之活化剂的存在下培养两周的人 成熟脂肪细胞
[0037] 的表型变化..................................................................96
[003引錐例4舰英光素酶活脚聊书R进行的高通量遍革麵选......................97
[0039] 实施例5.蛋白质组学分析.........................................................103
[0040] 嫌例6特异瞄晌AI旨肪细腳勺離IM麗倘妍錄嗦征........................1 说
[00川实施例7.表型、基因型和蛋白质组学纖集的统一...................................1(M
[0042] 实施例8.月腑的动物申觀.........................................................105
[004引 实施例a人IIP1和IIP2基因的核臟列和線物.....................................105
[0044] 表
[0045] 表1..............................................................................6
[0046] 表 2.............................................................................44
[0047] 表 3.............................................................................47
[0048] 表 4.............................................................................54
[0049] 表 5.............................................................................55 防胡 表6.............................................................................55
[0051] 表 7.............................................................................57
[0052] 表 8.............................................................................60
[0053] 表 9.............................................................................62
[0054] 表 10............................................................................63
[0055] 表 11............................................................................66
[0056] 表 12............................................................................78
[0057] 表 13............................................................................80
[0058] 表 14............................................................................82
[0059] 表 15............................................................................84
[0060] 表 16............................................................................84
[0061] 表 17............................................................................86
[0062] 表 18............................................................................91
[0063] 表 19............................................