人支气管上皮细胞恶性转化差异表达基因的cDNA文库及与肺癌病变相关的克隆的制作方法

专利名称：人支气管上皮细胞恶性转化差异表达基因的cDNA文库及与肺癌病变相关的克隆的制作方法
技术领域：
本发明涉及基因的cDNA文库及与病变相关的克隆，特别是涉及人支气管上皮细胞恶性转化差异表达基因的cDNA文库及与人肺癌病变相关的克隆。
BEP2D细胞系是经HPV18病毒永生化的人支气管上皮细胞，正常条件下可在体外长期传代，但在外界致癌因素的诱导下，可发生恶性转化，与体内的癌症发生极其相似，是进行人支气管上皮细胞癌变研究的好材料。BEP2D细胞癌变系已于2001年10月8日保藏于中国微生物菌种保藏管理委员会普通微生物中心，简称CGMCC，保藏号是CGMCC №0641。
人支气管上皮细胞恶性转化前的差减cDNA文库，由序列1至序列42组成。
人支气管上皮细胞恶性转化早期的差减cDNA文库，由序列43至序列77组成。
人支气管上皮细胞恶性转化时期的差减cDNA文库，由序列78至序列105组成。
人支气管上皮细胞恶性转化前与肺癌病变相关的基因克隆为序列1、10、11和19。
人支气管上皮细胞恶性转化早期与肺癌病变相关的基因克隆为序列45和66。
本发明的研究均利用BEP2D细胞系的细胞进行。细胞用HLC-8无血清培养液于37℃，5％的CO2培养箱中培养。该细胞经1.5Gyα粒子照射并连续培养至20代(R15Hp20)时开始出现细胞形态学的改变细胞间失去接触抑制，呈密集复层生长，细胞骨架排列紊乱，细胞增大且出现异形性，核质比例增大，核仁异形突出，细胞器发达，体外生存能力增强，可在软琼脂中形成克隆等，但接种裸鼠不成瘤，说明细胞发生了形态学转化，是恶性转化早期。第35代(R15Hp35)的细胞接种裸鼠后成瘤，组织切片证实该瘤为人上皮来源的鳞癌，说明细胞已发生恶性转化。由此可见，BEP2D细胞体外恶性转化与体内癌症的发生一样，是一个渐进的多阶段的连续发展过程。以该细胞系为对象进行研究，对于深入了解肺癌发生、发展和恶性转化的分子机理具有重要价值。本发明文库对于保存永生化人支气管上皮细胞恶性转化不同时期差异表达基因以及寻找参与肺癌恶性转化、肺癌诊断及其它肿瘤相关基因具有重要意义。筛选出的克隆，使从分子的角度进行癌变的各期诊断成为可能，还可以用于肺癌发病机理研究及药物靶标的寻找。
下面结合具体实施例对本发明作进一步说明。
抑制消减杂交(SSH)的具体过程如下1、细胞总RNA提取细胞常规培养达指数生长期2×106时，采用Trizol(Gibicol)试剂分别提取永生化BEP2D细胞、照射后R15Hp20和R15Hp35细胞的总RNA。
2、抑制消减杂交(SSH)具体操作按SMARTTMPCR cDNA Synthesis Kit和Clontech PCR-SelectTMcDNASubtractive Kit说明书进行。
3个文库的差减方案为人支气管上皮细胞恶性转化前的cDNA差减文库是以BEP2D细胞的cDNA为被差减cDNA，R15Hp35细胞的cDNA为差减cDNA，进行差减杂交；人支气管上皮细胞恶性转化早期的cDNA差减文库是以R15Hp20细胞的cDNA为被差减cDNA，BEP2D和R15Hp35细胞的cDNA混合在一起作为差减cDNA进行差减杂交；人支气管上皮细胞恶性转化时期的cDNA差减文库是以R15Hp35细胞的cDNA为被差减cDNA，BEP2D细胞的cDNA为差减cDNA进行差减杂交。
3、差减cDNA文库的构建(T/A载体克隆法)从PCR扩增后的差减基因产物中分别取出3 l，与1μlpGEM-T载体连接，4℃过夜。从连接体系中取4μl转化受体大肠杆菌DH5α，接种于X-gal/IPTG Amp琼脂平板上培养，挑取白色克隆在5ml LB培养基中37℃振摇培养过夜，加15％甘油，液氮速冻，-70℃保种。
4、文库的鉴定和筛选(测序和PCR法)测序鉴定随机挑取文库的克隆，对插入的cDNA片段进行单向测序。测序结果与GenBank中序列比较，17.76％的克隆未查到同源序列，消减库中约86.37％的克隆代表着不同的基因，表明cDNA消减群体内部很少重复拷贝，不同基因间丰度的差异得到了很好的均衡。
PCR鉴定用与接头有互补序列的内引物(巢式引物)1R和2R对质粒进行扩增，取5μl菌液于5ml MS液体培养基过夜培养后，再取1μl培养液直接作模板，然后依次加入10×PCR buffer 2.5μl，巢式引物1R和2R各1.0μl，Taq DNA聚合酶0.5μl(2.5 U)，50×dNTP 0.5μl，H2O 18.5μl，于94℃ 30s、68℃ 30s、72℃ 1.5min，30个循环扩增，反应结束后用1％琼脂糖凝胶电泳检测扩增产物。因为只有插入片段才连有接头，所以在电泳图谱上可见扩增产物者为阳性克隆，而无扩增产物者为空载质粒。
PCR筛选以pGEM-T载体两端的一对通用引物T7/SP6扩增了1316个克隆的外源插入片段。经1.0％琼脂糖电泳鉴定，除其中13个克隆没有扩增出插入片段外，其余克隆都得到了特异扩增片段，外源插入片段的大小大部分介于500-1000bp之间，平均大小在750bp左右，与SSH设计预期的插入片段大小吻合。本发明3个cDNA文库共获得1303个cDNA克隆，其中永生化细胞恶性转化前416个、恶性转化早期(形态学变化但不成瘤)301个、恶性转化(癌变)时期586个，其中序列表中的105个克隆是已测序鉴定的部分。
实施例2、筛选出与肺癌发病相关和可用于肺癌诊断的克隆以上述文库克隆中的基因为筛选的靶基因。
从临床上选择如下病理材料15例肺癌组织、5例肺癌旁组织和肝癌、胃癌、食管癌、乳腺癌、白血病、子宫内膜癌、脑神经角质瘤和结肠癌各3例。
采用cDNA微矩阵(芯片)方法，具体过程如下1、芯片制作将1303个克隆按如下方法制作成芯片，取2.5μl原菌液于5mlMS培养基过夜培养后，再取1μl培养液作模板于94℃30秒、55℃30秒、65℃30秒30个循环扩增，然后用QIAquick PCR Purification Kit纯化、真空抽干，最后以0.5ug/μl的浓度溶于12μl 50％的DMSO。在100℃变性5分钟后，移入96孔板并用点样仪点布于醛基化的玻璃片上。
2、探针制备用Trizol(Gibicol公司)试剂，按其说明提取各病理组织标本的总RNA，用DNaseI消化去除gDNA后再抽取总RNA。取1μlg用SMART PCR cDNA Kit逆转录，然后将逆转录产物用LD PCR标记成探针(80μl水、10μl 10×Advantage 2 PCR buffer、2μl 50×dNTP(final conc500uM dATP、dGTP、dTTP、dCTP 500uM)，1μl 25nM Cy5或Cy3标记的dCTP、4μl PCR Primer、2μltemple；循环参数95℃5秒、65℃5秒、68℃6分钟，18个循环扩增)。接着用QIAquick PCR Purification Kit柱子纯化、真空抽干，然后以50ng/μl的浓度溶于水。取6μl在100℃变性5分钟后，迅速置冰上，然后加入10μl UniHybTM杂交液并混匀。
3、杂交及洗片用移液器将探针迅速移于基因芯片上并用盖玻片轻轻盖住，置杂交盒内于62℃杂交8-10小时。杂交完毕，分别用1×SSC+0.1％SDS、0.1×SSC+0.1％SDS、0.1×SSC和水各清洗两分钟。
4、图象扫描及分析芯片置ScanArray 3000扫描仪内扫描，ImaGene软件分析和处理数据，以持家基因β-action的密度值作为参照进行校正。按如下公式计算基因的表达丰度 以表达量高于对照组10倍以上为标准进行差异表达基因的筛选，结果是以肺癌旁组织为对照组时，肺癌组织中高表达的26个，其它8种癌组织中高表达的12个；以肺癌组织为对照组时，肺癌旁组织中高表达的31个，其它8种癌组织中高表达的13个；以其它8种癌组织为对照组时，肺癌旁组织中高表达的63个，肺癌组织中高表达的87个。
筛选出的与肺癌发病相关和可用于肺癌诊断的克隆，细胞恶性转化前是序列1、10、11和19；细胞恶性转化早期是序列45和66。
序列表<160>105<210>1<211>540<212>DNA<213>人属人种(Homo sapiens)<400>1tccattaaat gctcccgccg ccatggagga cgcgggaatt ccatttcgag cggacgaccg60ggcaggtacc atcttgactt ccccagttac attcacctgg tctggtctct gtaagcttag 120aagagtagaa tcactttatt ttttaaagac ttccggccag gcacggtggt tcacgcccgt 180aatgccagca ctttgggagg ccgaggcggg tggaccacct gaggtcagga gttcaagacc 240agcctggccg acatggcgaa accctgtctc tactaaaaaa aaatacaaaa agattagctg 300ggtgtggtgg tgtgtgccta taatcccagc tactcaggag gctgaggcag gagaattctt 360ttaacccaag aggcggaggt tgcagtgagt caagatcaca ccactgcact ccagcctggg 420cgacagaatg agactctgtc tcaaaaaaaa aaaaaaaaag aaaagtacct cggccgcgac 480cacgctaatc actagtgaat tcgcggccgc ctgcaggtcc gaccatatgg gagagctccc 540<210>2<211>600<212>DNA<213>人属人种(Homo sapiens)<400>2atgctcccgg ccgccatggc ggccgcggga attcgatttc gagcggccgc ccgggcaggt60acatatgaga acacagatac tacattgccc aatgtcatca actagaacag caggaatctg 120tgtgctggga agcctgaccc cagaaccagt gctctcaaat ccccaccaat gtcatctagt 180ggttactgga gtccttattt tggagtcctg atatccatgc taccaattcc tatctgagtg 240gcaatgaaca tgtcacttcc ctttctgagt tggcttccac actcacagga tggagactga 300gataccattt tgctagggtg ttatcaagat tcagagatcc aaagttacac ttaaaagtca 360tttgagacat ttgtcttttt ttctacccca ccccctgcct aaaaggttct cctcttaccc 420aggatgaact caagatgtaa cattttaaaa tgagccttag gaaaaaacct gcaagggttg 480gtacctcggc cgcgaccacg ctaatcacta gtgaattccg cggacgcctg caggtcgacc 540atatggggag agctaccaac gcgttggaag cataacttga gtattctata gtgtcaccta 600<210>3<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>3atgctcccgg ccgccatggc ggccgcggga attcgattag cgtggtcgcg gccgaggtac60tcatttttta ccttgtaggg agggtgggca gtggggttac ctgaccttat ttgagggtat 120gggctttctt tttttatttc atcacttgtt atctcaaaga gactcggagc cagtgatcct 180tttatcctgc tacagtcttt agggagctaa aaaaaaaaaa aaaaaagcag gggctgccaa 240aactcttgat ttcatatttc cttctctaaa tatatatgta tcctgttttt ttggataaaa 300ttttaccaag aatccaaaaa aaaaaaaacc ctagaattta atcaacaaga tcagtctaca 360ggtcacagtg gatttctttt caaactgaca atgtttaggt tttaagcaaa taaagttcca 420gttaatgtga aactcagtca caaagagttg agatttttcc tttatgaaat agaattgaca 480ttcttttatg ctataaatgt gcattcaggt cccattaacc atgctctgct tttatttggg 540gatagaacat tttctttttc atatcccaac cttcccactt cttcatagaa atgtgataag 600<210>4<211>660<212>DNA<213>人属人种(Homo sapiens)<400>4atgctcccgg ccgcctggcg gcccgcggga attcgatttc gagcggccgc ccgggcaggt60actaattgat cctttgttgc tttttatttt aaatagtcaa ctttttactc ctgctatgaa 120ttcacacgta taaatttcag gttgactaga gaggtaacta gagaaaagga agccatgaaa 180taacttaaag atggccgagg tgggtggatc atgaggtcag gagttcgaga ccatcctggc 240taacacggtg aaaccccatc tctactaaaa atacaaaaaa attagccagg cgtggtggca 300cacacctgta gtcccaaagt gctaggaata caggcgtaag ccacctcacc cagccaggtg 360aattttcaaa gccaacttaa tcccaataaa atattttgca agttagagtt tttaattttg 420gaagctactg ttttaaatat aaaatgagat gagatgttgg cacaaatatt ttcttttggg 480attttctttt aaatgccata gaatgcctct taagataaca aagaattgtt ggcatacctt 540acgtacctcg gccgcgacca cgctaatcac tagtgaattc gcggtcgcct gcaggcgcca 600tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa 660<210>5<211>660<212>DNA<213>人属人种(Homo sapiens)<400>5atgctcccgg ccgcctgcgg ccgcgggaat tcgattctag cggtcgcccg ggcaggtaca60aggctctcct gaatgatcct ttttttattt tttagtatac atcacaaatt cttgcatgag 120tgcagctgga agtaaattcc aattcaaatt ccctctgtta gtaaattcca attcaacaga 180taaatatttt tgtcttaaga tgcttaaagg gaatgactgt ccttgtaaga gtcaactgaa 240gcacagaaca ggttaatcac ttatcacatg tcttccttca gctacaagaa atggcattac 300acgccaagaa ttcctattca ctaactagtg acttgctgga tgtacctcgg ccgcgaccac 360gctaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg 420cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg 480tcatagctgt ttctgtgtga aattggtatt cgttcacaat tccacacaac atacgagccc 540ggaagcataa agtgttaagc ctggggtgcc ttatgagtga gctaactcac attaattgcg 600ttgcgctcac tgccgctttt cagtctggaa acctggccgt gccactgcat taatgaatcc 660<210>6<211>600<212>DNA<213>人属人种(Homo sapiens)<400>6tgcgtcgacg ctcccggccg ccatggcggc cgcgggaatt cgattagcgt ggtcgcggcc60gaggtaccat gtatgtcaca tggataatat ctttataagt ttttaacagg taataagaaa 120taaactaact ctccattaga aaactgggca agagtgctca gaagaaaagc aaatgaaata 180agcatataaa aaatgtttga tttccctagt agtgataagc aaaatgtttt acctgtcact 240ttggcagact tttgtttaaa aagaaaaccc atgacggaga gagtgctgct aaatgcctgc 300ttttaatgta ttacaagtaa atgtatggga gtgtaaattg gcacagcctt cctgaaaaag 360cacatctttt gacccactaa atcctcttca aagaatttat cctaaggatg taattagaga 420tggagacaaa gccttatata tcaagaatgc ttattataat attattgagt aataacagca 480aaaataaaaa aaaccgtggt aaaatgtctt acagttggga agtgcctaat gaagtatgat 540ttatccatac tatgtaatat tacataccat caaaaatcat atttaaagat aatgaccttg 600<210>7<211>600<212>DNA<213>人属人种(Homo sapiens)<400>7cgcgtcgcat gctcccggcc gcctggcggc cgcgggaatt cgatttcgag cggccgcccg60ggcaggtact ttctcctttt cctttttttc tatttagacc aaaaaaaaca aaaacaaaaa 120caaaaaaaaa caaaataata caaaacgaaa aaaaaagaaa gaacacccgt taacacacac 180gcgcacacac acacaaaatc tgtccatttg ccggaggcaa ttgtatgtat gttagttgga 240gggtattaac aaatcagttt tattccaaag atttaaaact agacatgact taaaaacaat 300ttctggagca ctgcttgctg acaatctcgt agttctctgc tgcatttgag tgcattttgt 360ggccagtcca tcagggcgta cctcggccgc gacacgctaa tcactagtga attcgcggcc 420gcctgcaggt cgaccatatg ggagagctcc ccaacgcgtt ggatgcatag cttgagtatt 480ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 540tggtatccgc ttacaattcc acacaacata cgagccggaa acataaaggt gtaaagcctg 600<210>8<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>8cgcgacgctc ccggccgcct ggcggccgcg ggaattcgat ttcgagcggc cgcccgggca60ggtacttttt tttttttttt tttttttttt tggaacaaaa caggtatatt tctattagac 120tacctgatac agaagatagt gtggtatgga tggatagtat gaaggacaaa taatacaaat 180atattttatt gaaataaaca aaaatgcata cacagctcaa tgggtcactt ggaacaaact 240tgcttgacta tattactgag cagaaacaaa acggaagcac aaacaccttt ctttttttga 300catgtatctg gagataggta ggaagacact acttggtttt ttaaaaaact gaccttccct 360taaggcctgg tcatagaagt gtaaacaatg taaatgaatc caccattacc agttgtcata 420tcatatctat gtcacctgtg tattctgaga ttacacacat acctgccaat atacctggga 480aagggtattt tatcacagtt acacttgagt tcttggcagg caggactgag gaatagtaat 540ttgaaagaag ttttacatcc tatttataag aaatcactag tatttcctta aataacaggg 600tacaatagaa agatctggct ggaagttatc ctttcacttt tgggtcattt ttaggttttc 660<210>9<211>660<212>DNA<213>人属人种(Homo sapiens)<400>9gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtacct60tttttctgag ctctggtttg cctttcttga ctgtggccat caccatgtca cccacaccag 120cagcgggaag tctgttcagc cgtcccttga tccccttcac ggagatgata tacaggtttt 180tggctcctgt gttgtcagca caattgatta ctgctcctac cggaagaccc aaggaaatcc 240ggaatttcgc accagaggac ccaccacgtc ctcgcttcga catcttgaac gccggaaaaa 300agagaaaaaa aagaaagtac ctgcccgggc ggccgaatca ctagtgaatt cgcggccgcc 360tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat 420agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttctgtgt gaaattggta 480tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 540ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt ttcagtcggg 600aaacctgtcg ggccagctgc attaatgaat cggccaacgc gcggggagag ggcggtttgc 660<210>10<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>10cgcgatgctc ccggccgcca tggcggccgc gggaattcga ttcgagcggc cgcccgggca60ggtacgcggg gttgctggtg acagcaaaaa tgacccacca atggaagcag ctggcttcac 120tgctcaggtg attatcctga accatccagg ccaaataagc gccggctatg cccctgtatt 180ggattgccac acggctcaca ttgcatgcaa gtttgctgag ctgaaggaaa agattgatcg 240ccgttctggt aaaaagctgg aagatggccc taaattcttg aagtctggtg atgctgccat 300tgttgatatg gttcctggca agcccatgtg tgttgagagc ttctcagact atccaccttt 360gggtcgcttt gctgttcgtg atatgagaca gacagttgcg gtgggtgtca tcaaagcagt 420ggacaagaag gctgctggag ctggcaaggt caccaagtct gcccagaaag ctcagaaggc 480<210>11<211>360<212>DNA<213>人属人种(Homo sapiens)<400>11agcgttgcgt cgcatgctcc cggccgcctg gcggccgcgg gaattcgatt aagcgtggtc 60gcggccgagg tacttttttt tttttttttt tttttttttt ttcttttttt tttttttttt 120tttttttttt tttctctaaa acttttttta aaagtttttc tcctctccgt taataagggg 180tttgattaat gctttgggcc attttttgca ggcctgccca ttgccggcaa tggactttga 240aaaatccctt ttcctggccc ccaaaagtta aatttctttt ttaaaaatat tccgatttcc 300ccaattcttg caaaagtttt acttgcccca ttttgcctcc tttttttaaa tcaaaacttt 360<210>12<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>12cgtcgcatgc tcccggccgc catggcggcc gcgggaattc gatttcgagc ggccgcccgg60gcaggtactg ctataaaact cattcttgtg tggtgttctg tgctatagag tctgtgtatt 120gctgttcata ttcggagttc tggttttgtt tttcccttaa aacctgttaa cagttttttt 180tgggggtggg gggattcaga actcttgttt cccattccat agcacctgac attatttcaa 240gttttatatt atcttaaggt gtatatttta ttttttttat tggcttagtt gttttttgtt 300ttgttttgtt tgagatggag tttcactgtt gcccaggctg gggtgcaatg gtgtgatctt 360ggctcactgc agcctccacc tcccgagttc aaatgattct cctgcctcag cttcctgagt 420agctgggatt acaggtgcat gccaccatgc ccggctaatt tttatatttt tattatagac 480gggatttcgc catgttaacc aggctggtct cgaactcctg acctcatgtg aatctgtccg 540ccttggccct cccaaaagtg gttgggaata caaggcatga gccacctatg cctggccctt 600<210>13<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>13cgcgatgctc ccggccgcca tggcggccgc gggaattcga tttcgagcgg ccgcccgggc60aggtacttga ttgttctgaa attccttatg catgattaga gaaagagtaa atgacgtatt 120ttagggttgt gtttaacaaa agtgagatgc taattcttga aaattaggcc tctctatggg 180gactgcacgt aatcacatta ccctagatct tgagttagcg agagaatatt cactgtcagg 240tgattcactg accttgccta taacttgaaa ccaatagcaa atccagatgt agtttcactg 300gcctttgaag tccagggcaa aaagacaaag cgtgactccc tgtctcccca ttgcatcctg 360attgtctccc tgtccaggga cagaataaat atcctcaaga cattgactct tttcacagtg 420aagaagctga ggttggagct ttggcctcat ctcctatagg ttcatgctgc atctctgatc 480tctacaggca gggagaatgt ctttcctgct tccctttctt ctggcacatg acaaaatcct 540agtgccttaa tgtctgcatg aggagagagt ttatatagtg ttttccagtt caaggtcctt 600atcatataat catacttccc ccacctgcat cttctttggt acactgtgca catatttaac 660<210>14<211>480<212>DNA<213>人属人种(Homo sapiens)<400>14tgcgtcgacg ctcccggccg cctggcggcc gcgggaattc gatttcgagc ggccgcccgg60gcaggtacat gctccaattc aaccccaggt ggctcttttc ctcccgtttt ttcctattgg 120tttaatgctt agctatctca aattctgtat gggccaggag tccctgaaac atatacaaat 180taggtcccct ttgtgaccaa cgtgctgtta ggcagctaat ctatttatca tctatttaat 240gttcatctag atcaatcagt cttctcaatc ttgacatgca ttttgggctg cataattctt 300tgttgatggg gactgtccta ttccctatag aattaacccc tggattctac ttatgtcagc 360agcactccct ccaattgtga caactacaat gtcttcaaac attgccagaa gtcccctgtg 420gggcaaaact gattgaaaac tgttactgta gattcaggca ggcccaccac tacccctatt 480<210>15<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>15ccctgcgtcg acgctcccgg ccgccatggc ggccgcggga attcgatttc gagcggccgc60ccgggcaggt acatttgaac cgtgccacaa atgaagaggc tgagtctgtg gcccgctctt 120tctttgctac acagatttgc tagacagggg ttaaagatca tcgaacatca aactgagata 180agtcagaagg cttggaagag aactgcaatg agacaaactt ttcccactgt gtgatgcaga 240aggattgata ttgcctctct gccacctaag atcctcccct gtatcatggt gttgggtgga 300ctacagcttt aggaagccaa cgtcagacta gtgtggtgcc tggtccttca gattggctga 360aggaagagac tgaagaatga ggcttaagtt ctcattggtg agatggggat atgaaatagc 420atgtatttac taccagtgtt gtggggagaa aaagaaaaga aaagaaaaga atggaaagtg 480cccacaaatg tgcctggtgc ttaatagatc tatttgcagc ctggagaaga gagctgtggt 540cacttgaaat ataaagatta tccttatcca tttaactggc ttactccagt gcctaagatg 600<210>16<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>16gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtacac60tgtctccttt aaggaaggaa gcgtgatgaa tgaatgatgt gtagacttga gggatgacta 120ttaaagggga cgtaggatga agagaaagaa cctacagatg acaatgaatg taaacttatt 180tttcttcatg tgtaagcagt gtgctcgctg gtgatatcca gatcctaaca agattacttg 240gttagctggt taggaccagt aactggattg cgaccactat gataatattt tgaaccaaat 300gttaatgctt gatgcagaat tgtaaagcag catctggttc ctatatagcc ttaaggatta 360attttagtga tcctcaagga attaaatagg gaatttcaga aatgtagact gcaaaggcag 420tatacaggaa aaggtggagt gggttttgtt tatgagggtg tctgaaaact aaaattgagc 480gggatatcat ggtatagttg gacagtattg gtccttcaca ctttggccat attgtataat 540ggagctttta ccaaagatgt atgagaagtg taagactatt aaaaaatgaa ctattcaaag 600taaaactctt aacaaacatt ttacttaaag cagatgcaaa agggtattct catgtagctc 660<210>17<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>17gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtacat60ttttttcttt ttcttttttt ttttttttta acaaagtctg aactgaacag aacaagactt 120tttcctcata catctccaaa ttgtttaaac ttactttatg agcgtttgtt tagaagttcg 180gaccaacaga aaaatgcagt cagatgtcat cttggaattg gtttctaaaa gagtaaggca 240tgtccctgcc cagaaactta ggaagcatga aataaatcaa atgtttattt tccttcttat 300ttaaaatcat gcaaatgcaa cagaaataga gggtttgtgc caaatgctat gaacggcctt 360ttcttaaaga caagcaaggg agattgatat atgtacctgc cgggcgggcc gttcgaaatc 420actagtgaat tctcggtcgc ctgcatgtcg accatatggg agagctccca acgcgtttgg 480<210>18<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>18gcgtcgacgc tcccggccgc catggcggcc gcgggaattc gattagcgtg gtcgcggccg60aggtacgtgc cctgctgggg gccttacttt atagaatgag agcatccgag cttccctaat 120gaatctggct agttctgtgt ctggctgagg atacaggagt gggacatcca ctctcggatc 180cctcagagca cagaaacctt cagctttgct gtctctgaag tatttcctcc agtttccctg 240cgggccccta tgtttgagtt tgatggctgc tggatcctca ctcaacgaaa actcggttgg 300aaactgttcc gcctggcagt ccttttttgt tgttttccat ctcatttccc ttccatctga 360aagtggcatt cagctgacct gctcatttag actgttcacg gagtctgaat ctgccaacgt 420ggtgttggag gctccacctt gaaaagggcc acagtcaggg caactttccc catacaggaa 480aacttgaaaa ttacatcaac agtctacgtc acaagcaaat tatatttcct ttataccaaa 540caaaactatg gagaactaaa agtacctgcc gggcgggccg ctcgaatact agtggattcg 600<210>19<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>19cgcgatgctc ccggccgcct ggcggccgcg ggaattcgat tagcgtggtc gcggccgagg 60tacctttttt ctgagctctg gtttgccttt cttgactgtg gccatcacca tgtcacccac 120accagcagcg ggaagtctgt tcagccgtcc cttgatcccc ttcacggaga tgatatacag 180gtttttggct cctgtgttgt cagcacaatt gattactgct cctaccggaa gacccaagga 240aatccggaat ttcgcaccag aggacccacc acgtcctcgc ttcgacatct tgaacgccgg 300aaaaaagaga aaaaaaagaa agtacctgcc cgggcggccg aatcactagt gaattcgcgg 360ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat 420tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 480ttggtatccg ctcacaattc cacacaacat acgagccggg agcataaagt gtaaaagcct 540ggggtgccta atgagtgagc taacttacat taattgcgtt gcgcttactg ccccttttca 600<210>20<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>20gctcccggcc gccatggcgg ccgcgggaat tcgatttcga gcggccgccc gggcaggtac 60tgaaattagt agaagctagg ccaatgatcc atgaattgtt aactgaaggg cggagatcga 120agactaacaa agcaaaaacc cttgctacat gggcaacaaa agaactgagg aaactgaaga 180accaagcttg atctgttacc attgggatga taacctgagg acccccactg gaaatctccc 240atcttttgaa aaacctggaa gtgaggagtg tgcacggatg ctgaatgttt gggaatgaga 300ggatgagtga gtgaggcttg aaaacacacc acattgaaaa tcctgccaca gcagcagccg 360cagccgccaa cagcagcgct gttagtgagc taagtaagca ctgacttcgt agaaaaccat 420aacatcggcc atcttggaaa agagaaaaac aatggagtta cttatttcaa aaaaaaaaaa 480aaaacaaaaa aaccgtcctc ggccgcgacc acgctaatca ctagtgaatt cgcgggcgct 540gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata 600<210>21<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>21gctcccggcc gcctggcggc cgcgggaatt cgattagcgt ggtcgcggcc gaggtactaa60atgtatctaa agaaacacac agtcctacaa aagttgttct cagagaaata tcattgaggt 120gggggcagct cttcccagga tgctaaaagt tctatatgat ataagcacaa attaacagct 180tgatgaagac ataatctact gcagctttga gaaacctatg cctgggatgt agttacccct 240cacattctac aatgttgtag agattttttt tcccaagaaa atgtcatttg aaacatattt 300acaactgaac tcaacagaga ctgtgaaatt gaacaggcac tgctcatttg tttgagtttt 360ttggtaaaca ttttgctggt tttttttttt gtttgtttct ttcttgtttt gcatcaactt 420ttatcaagtc catgcaactt cagtgccctc gatgaaggat gcataaaaag ccatacttct 480<210>22<211>420<212>DNA<213>人属 人种(Homo sapiens)<400>22gctcccggcc gccatggcgg ccgcgggaat tcgatttcga gcggccgccc gggcaggcac60tttttttttt tttttttttt ttggttttta ttctgaccaa agcatttatt ttcattccat 120ttacatagga ggtggcattg cacaaaataa aaactttctc aaagagatat gaattaaaga 180aaagttagag taaggcgaat acttatttaa acataccttt agagtgaggg taagccccct 240tcccaaccca aaatgaactg aatgagacag aaaaagaggt ccagagtact ttgcggagcg 300atgttcccat ctctaatgcc actcagctca ggagcaggct tcacgaccct gcctttatct 360ttacacaaag ccatgggaag ggaaatgtct gaggtaacaa ctcaccgttg ggcatcatat 420<210>23<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>23gcgtcgcatg ctcccggccg cctggcggcc gcgggaattc gatttcgagc ggccgcccgg60gcaggtacgc ggggttcttg aacaacatac aaagtttaaa gtaacttggg gatggttaca 120aaaagttaac acagtatttt tctcaaatga acatgtttat tgcagattct tcttttttga 180aagaacaaca gcaaaacatc cacaactcag taaagctggt gggacctaat gtcaccttaa 240ttctgacttg aactggaagc attttaagaa atcttgttgc ttttctaaca aattcccgcg 300tattttgcct ttgctgctac tttttctagt tagatttcaa acttgcctgt ggacttaata 360atgcaagttg cgattaatta tttctggagt catgggaaca cacagcacag agggtagggg 420ggccctctag gtgctgaatc tacacatctg tggggtctcc tgggttcagc ggctgttgat 480tcaaggtcaa cattgaccat tggaggagtg gtttaagagt gccaggcgaa gggcaaactg 540tagatcgatc tttatgctgg tattacagga gaagtgacat actttatata tggttatatt 600aacaaggtct ggttttaata cccatatact ttatatttct atacatttat atttctaata 660<210>24<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>24gctcccggcc gcctggcggc cgcgggaatt cgattcggcc gcccgggcag gtacatatgc60tgccgttatc attttaccgg caaaccacct gccatgcaat gcattgacag cagcaatagc 120tgcagcaatt gatgggcact tcacatacac attgccctga gctgaatttt tgtcaacata 180aatatgaata actcctccat gtttattaca ttcttcaatc acatcatcct taatctcggt 240atcccatcca acttcttctt ctgtttgagg gttaaacatg ttagagagtt ggaaacattg 300tgttgcaagt ggctgaacag aggcagctgc agctaaagct gaagcttcag tctgctggga 360aagtcttgtt tgcaaatctg ccacagcacc aaatgccaaa gagccactca tctgtacagc 420ttgctgtgct gctggcggaa tcagcaaacc tgtcctcggc cgcgaccacg ctaatcacta 480gtgaattcgc ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca 540tagcttgagt attctatagt gccacctaaa tagctttggc gtaaatcatg gcatagctgg 600<210>25<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>25gctcccggcc gcctggcggc cgcgggaatt cgattagcgt ggtcgcggcc gaggtacaac60cagccagatt ccaagcggcg ccagaccaat aatcagaact ggggctccca acccattgct 120cagcaaccgc tccaaggtgg tgatcattct ggtaactatg gttacaaatc tgaaaaccag 180gagttttatc aggatacttt tgggcaacag tggaagtaga agcagtaggg cctctgtaaa 240attggagact gataggttga tcagaaactc accctaaatc tgaacgggtg ccgctataat 300ttgtgacatc tggcaagatt tccctttatg tatatatttt aacaatccgc ttggacacga 360acaaggccac acttctaact gcttctggcg aactgatttt atttttaatt tttttcaata 420aagatattct tagatactgc aaaaaaaaaa aaaaaaaaaa agtacctgcc cgggcgggcc 480gctcgaaatc actagcgaat tcccggccgc ctgcaggtcg ccatatggga gagctccaac 540gcgttgggat gcatagcttg agtattctat agtgccacct aaatcgcttg gcgtaatcat 600<210>26<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>26gtcgatgctc ccggccgcca tggcggccgc gggaattcga ttagcgtggt cgcggccgag60gtactcagga actaggccat ttggcagaaa actgtagcaa aaaagcaatc cttggcttga 120ctctggcaaa gtctgaatgg gctaaaagcc aaaaggaaaa gctgggttat ctgaatgaca 180gccttcagat cctgactaac actttggaaa aacttctcgt ttcttattca taatagaata 240agatctccag cagttacatg tgtgtgaaga ttatctgagg agctctgtta gcccctaaac 300caaatgcagg gtttgaggcc aatgcccttt ggttagacag aaccacactg cccagatggc 360tagagaccac ccagataact caatccatca ttaagttatt gtaagcagca aactgtaagc 420agaagtggat gatgaagatg gaaaaaaata taaagatagg gcctgtccct ggcaaactaa 480gaacctgaat ttcagacctc tctgaaatag gtacctgccc gggcggcgct tccaaatcac 540tactgaattc gcgggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat 600gcatagcttg gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatact 660<210>27<211>300<212>DNA<213>人属 人种(Homo sapiens)<400>27aaacaaaacg gatgcacaaa cacctttctt tttgtgacat gtctcgggcg ataggccgga60acacactact tggtttttta aaaaactgac cttcccttaa ggcctggcca tacaagtgta 120aacaatgcaa atgaatcccc cattaccagt tgtcatatca tatctatgtc acctgggtat 180tctgagatta cacacatacc tgccaatata cctgggaaag gctattttat cacagttccc 240cttgagtttt tggcaggccg gaccgcggaa gagtaatttg aaagaagctt tacctcctat 300<210>28<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>28gctcccggcc gccatggcgg ccgcgggaat tcgatttccg gcggcccgcc cgccgcccac60aacgcaccct gctgatccgc accttctcac caacacatat aacacccacg caccgtcacg 120cctctatatt gcggtgctcc ggccctgtca taagtctcac tccccagtgc aaaatccata 180atgctatccg ctgatccaac tcactcaccg ctttttacag ggcatccagc tccctgagag 240ctcagctcta agtcgaacgc acggttgtca ctcaacaagt gaaggaccga ccaaacgctc 300tttgtgtgaa gagcttcaaa ccttggccct ctctgctaag gtaatgtcct ttgctgccgg 360ctaagctacg aaaagtgaaa ggcaaattct caaatctgta gactccgtaa ggcaaacaca 420caaactctgt acctgcccgg gccggaccct aaagagcacc ccccgccgcg cctactgagc 480cgagcttact cacattaatt ggcgttgcgc tccctggccg gtttccaatt ccgggaaacc 540ttgccgcccc ccttgcatta atgaatcggt ccaccccccg gggagaaggc cggtttgcgt 600<210>29<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>29gccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgatta gcgtggtcgc60ggccgaggta cctggagaag atcaaacagc gactgtttga gaaccttaga atgctgccgc 120acgcacctgg ggtccaaatg caggcgattc ctgaggacgc catccctgag gagagtggcg 180atgaggacga agacgaccct gacaagcaca tctcgatctg ctcctctgac aaacgaattg 240cctgtgagga agagttctcc gattctgaag aggagggaga ggggggccgc aagaactctt 300ccaacttcaa aaaagccaag agagtcaaaa cagaggatga aaaagagaaa gacccagagg 360agaagaaaga agtcaccgaa gaggagaaaa ccaaggagga gaagccagaa gccaaagggg 420tcaaggagga ggtcaagttg ggctgaatgg acctctccag ctctggcttc ctggctgagt 480<210>30<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>30aatgggccga cgtcgcatgc tccggccgcc atggcggccg cgggaattcg attagcgtgg60tcgcggccga ggtactttct ttttcttttt cttttttttt ttttttttag tttttgagat 120cgtgtctcac tcctgtaatc ccagctactc aggaggctga ggcaggataa ttgcttgaac 180ccgggaggtg gaggttgcag tgagccgaga tcatgccact gcactccagc ctgggcaaca 240gagtgagact ttgtctcaaa aaaaaaaaaa aaaaaaaaaa agatctggcg gatgaaaata 300accagaatga aaatagctag aaaactcagc aagcaggaag ctccctttct cacccttttg 360ttcccttgcc gatagaatca gtcactatta gaaaaaatga aagacgctct gtttaaaaca 420atgatgacag cagtacttgc cggggcggcc gctcgaaatc actagttgaa ttcccgggcc 480<210>31<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>31ctccccaatg gggccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat60tagcgtggtc gcggccgagg tacaaggcag tatttttgtg tgtttgacca aagcgcttta 120cttcatgtaa gagaaaaaat aaagtaaaac aaggccaggt tccagtggct cacgcctgta 180atcccagcac cctggaaggc caagacaggc ggattcccag aggagttgag accagcctgg 240tcaacatggc gaagccctgt ctctactaaa aatacaaaaa taagccgggt gtggtggcac 300atgcctgtaa tccctgctac ttgggaggct gaggcaaaag aatcacttga acccaggagg 360cggaggttgt agttgagcca agattgcgcc actgcacttc agcctgggtg acagtacctg 420cccgggcggc cgctcgaaat cactagttga attcgcgggc cgcctgcaag gtcgaccata 480ttgggaaaac tcccaacgcc gttggattgc attaaccttg gagttattct tattagtggt 540ccccctaaat taacctttgg gcggttaatc cattgggcca taaacctggt ttccctggtg 600<210>32<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>32tccccaatgg gccgacgtcg catgctccgg ccgccatggc ggccgcggga attcgattag60cgtggtcgcg gccgaggtac atttcacctg ttagtatgac catctttttt cttcggtggt 120ggaaggaagg gcgaggtgac tgtgtaaagc aaagatgtgg gaagtagaaa tattgtttac 180tagacattat aaagtcatgg tgaaaagaaa gcttcgatta atacatagcc taagggggaa 240aagactacac tccaacttca gaaaattaat ttagaaggat aggggaagag attaagtaat 300tgcagtttaa catgaaaaat gcacatgtga tagaatggag cacaatggag gattcataaa 360acatgctcat cagaaaaatc tgttaagtcg actttgtcca gattcagaaa gagtaagttt 420atcaacatct ggcatccctc atactttgtg gaagatagat cttctactgc ttaattattg 480agaaattatt ttattcaaaa gaaaaggagc tcttaatcct tttggttaaa acaaaattaa 540<210>33<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>33caatggggcc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattagcg60tggtcgcggc cgaggtactg tggaaaccaa atttgaatac tgcaaatttg taggagttac 120taggttagca attagtccat acatccataa gcctgatggg ttgaaattgc agtttgagaa 180gtgaattaac cttacatccc tttgttcaga taccttaaaa gttactttat ttaaaagcat 240ttattaatct tagtctgaaa tcaaaatata gattaattgg ctcagcttta atacctttct 300aggaggtgtc acaatgtagg gtacctgccc ggggcggccg ctcgaaatca ctagtgaatt 360cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat tgcatagctt 420gagtattcta tagtgtcacc taaatagctt gggcgtaatc atgggtcata gctggtttcc 480tggtgttgaa aattggttat gccgctcaca attgccacac aacatacgag cccggaagca 540ttaaagtggt aaagcctggg ggtggcctaa tggagtggag ctaactcaca tttaagttgg 600cgtttgcgcc ccactgggcc cgcttttcca agtccgggga aaacctggtc cgtggccaac 660<210>34<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>34caatgggccg acgtcgcatg ctccggccgc catggcggcc gcgggaattc gatttcgagc60ggccgcccgg gcaggtaccg tgttttaagc ttagttcagt ctcaagtgtt tgcagccaca 120tctgaagacc aataaagcaa ctgctggttt atcctttggg agctgacaga atttcttctc 180ccaaatacat acacagtaaa atcataagcc tgaattacct gtgaagttgc tttcagatca 240tatcgttggt tcactcttac agaaggatca ctttaaaata tctctgcatt tgtatccttg 300caatgccaca actaaaagac agttatctca taggtccttg attaaattag gaggcttttt 360attgaacaca agaaatgata cttttcaggc actggggcag ataacttgct agaaaggcat 420tttttaactg tgctaagatt gagctgagtg cgttggcttg gagacaccac acaacacgga 480<210>35<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>35caatggggcc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgatttcga60gcggccgccc gggcaggtac aaaggtggca ggtcaacccc actgtgggga aatcaaggca 120gaatccttcc cagaaaaggg atctggatgc aatcacagac atcagcccta agcaaagcac 180acatggcgag agagggcaca gatgcagcga ttgtggcaaa ttcttcctcc aagcctcaaa 240ctttattcag catcggcgca tccacactgg agaaaaaccg tttaagtgcg gagaatgtgg 300gaagagctac aatcagcggg tgcacctcac ccagcaccag cgcgtccaca caggggagaa 360accctacaaa tgtcaggtgt tgcggaaagg ctttccgggt gagttcccac ctggttcagc 420accacagtgt ccacagcgga gagaggccct atggctgcaa tgagtgtggg gaagaaactt 480cggtcgccat tccgcatttg gattcgaaca acctaaaacg ccacttcagg ggagaaattc 540ccagagattc agtgacaaaa gaagttaaga acccaaaaat ttaagtgtta agaaaaaaat 600<210>36<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>36ccccactggg ccgacgtcgc atgctccggc cgccatggcg gccgcgggaa ttcgattagc60gtggtcgcgg ccgaggtaca aaacattcat aagagaaacc tgcatacatt ttgatattaa 120gaaataattc cggggattct tccactcctg aaatgagttg atttgcagat aactcacaac 180ttcttaagct aaatggtatt ttcatttttc tcaagctctc caataaatat gaccaccaag 240atgcagaact ctttcaggac ttcttttgct ccattattct cacagatact tactgatttt 300tcttttcttt aaattggtgt ttccttgtag atttttttaa gtgcggtctt ctgtcttgtg 360aattatttaa agtaagcatg tcttaccttc acttagcaca catttgcagt gcacctactg 420tgtttgaggc aacaaggttt gagtgctggg gaaccaaaag tttttatcaa aaatggcttt 480cctgggacca gacatggggt ggctcatggc ctggtaatcc cagcactttg gggagggcaa 540ggtggggtgg gatcacctgg aaggtcaggg cgttttgaaa accaaccctg ggcccaacat 600gggtggaaaa gcccttttct ccttactggg aaaattattt aaaaaattta aaccccaggg 660<210>37<211>480<212>DNA<213>人属 人种(Homo-sapiens)<400>37caattgggcc gacgtcgcat gctcccggcc gccatggcgg ccgcggggaa ttcgatttcg60agcggccgcc cgggcaggta cacaaaccaa ctctttttct tataatttac aatttgttga 120aaaaattatt gttttgctgt tttcatccta ctaacctctt taacagaaca caatttatca 180gagcacaaag cttaaacttc ttatgatgat gcaacagaca cagccaccta caatggctga 240taaacaacag gtatgttaca cacactgatt ggaagaccat atcagaaaaa acagagtaag 300gcaccactct tgggaaatta aggtagcttg cagtaacaag tgttgagcac cataataagt 360aggtgctcaa taaatacatt gaatgaatga ttgaaagcca taattagctc tattctttta 420attgccagca attcttcaac ctcaacaaaa tacttatttt aaaaaaagat ttggtacctc 480<210>38<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>38cacaatgggg ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgatttc60gagcggccgc cgggcaggta cagaaaggaa aaatgaaagg tctttgaact atagtgatat 120agctttgaag catagtgata taagtgaaac aagcaataat aatacagcgt gggccgggcg 180tggtggctca cgcctgtaat cccaacactt tgggaggccg aggcaagcgg atcacttgag 240gtcaggaact cgagattagc ctggccaata tggtgaaacc ccgtctctac taaaaataca 300aaaattagcc gggcatagta gtgtgcatct gtagtcccag ttattcggga ggcagaggca 360ggagaatccc ttgaacccag gaagcagagg ttatagtgag ctgagatcgc accactgcac 420tccagcctgg tgagagagca agaactctgt ccaaaaaaaa aaaaaaaaaa aaaaaaggta 480<210>39<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>39tcccccgagt ccatgctccc gggcggcatg gcgggcgcgg gaattcgatt agcgtggtcg60cggccgaggt acatggccac caagtaagaa tggttggtga caagacagaa ggctaaaaca 120ggaaggtaat cttgtgcacc tgacaaatag aaagaataaa ggatcaaaat tgaaggcagg 180ctataagagt atcaagaaat ccttaaaaac caaaaagtga tttggaagca caaaacttac 240agttaatgct acccaatgtc atgatgggcc aagaacattg tggcttccta agttagaaaa 300tgccatatgc caaaatttta aatgggaaca cactacattt ttttctaatc aatccccccc 360tctcccagaa aaaataggaa ctcatttttt tcaggggtgg ggaggaagag aggggatcat 420ggggacatgg aaacagtagt tatattagtt agtatttttg gttattgata aattttggtg 480<210>40<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>40gagacagaat cttgctctgt tgcccaggct ggagtgcagg gacacctccc gggttcaagc60agttctcctg cctcagcctc caagtagctg ggactatagg cacgcaccac catgcctggc 120taatttttgt atttttagta gaaatggggt ttcactgtgt tagccaggat ggtcttgatc 180tcctgacctt gtgatctgcc cgccttgggc cttccaaagt gttgggatta caggcgtgag 240ccactgcgcc tggccatcag gtaaccttta tggctctttc aggaaaagtg aaatcagaga 300ggcgtacctc gggccgcgaa cacgctaatc actagttgaa ttccgcggcc ggctgcaggt 360cgaccatatt gggagagctc caacgcgttg gattgcatta gcttgagtat tctatagtgt 420cacctaaata gcttggcgta atcatgggca tagctggttg cctggtgtga aaatggttat 480gcggtcaaaa ttgcccacaa aattacaagc cggaaacata aagtggtaaa gctggggtgc 540<210>41<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>41tccgccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga tttcgagcgg60ccgcccgggc aggtaccgtg aattggctgt ggaagggatg cacagagaac ttgtgttccg 120gtttattgaa gttcagacac ttctcctcgc tccattctgt ccacatttgt gtgagcacat 180ctggacactc ctgggaaagc ctgactcaat tatgaatgct tcatggcctg tggcaggtcc 240tgttaatgaa gttttaatac actcctcaca gtatcttatg gaagtaacac atgaccttag 300actacgactc aagaactata ttgatgccag ctaaagggaa gaagactgac aaacaacccc 360tgcagaagcc ctcacattgc accatctatg tggcaaagaa ctatccacct tgggcaacat 420accaacccgt ctgttctacg taacactttg aggccaataa cggaaaactg gctgacaaca 480aagtcattgg ttagtgaact agcagtatgc caaaatgaag aaatacattg aagaaagtca 540<210>42<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>42ccgacgtcgc atgctcccgg ccgcatggcg gccgcgggaa ttcgatttcg agcggccgcc60cgggcaggta caaaaaaact agctcagaaa aggtcaaatc ctcctaaatc tagtttttct 120taacatctgg cttcttactt ttgggaacaa ggaaaacatt gccatctctt gtttgctgca 180gggagtattc actaggagaa taaggttttc catcttcatc acgtagcatg ctgaaaactt 240cgagatataa ggtgctgagt tgttttttca gtaggtgaag gcttttgtca ttttctcctt 300tttcttcgag caatttttct ttttcatctt tcaaatgatc taaatcttgc tctagttcta 360ctatattttc cagttttctt tttctgcaat tctgagcagc cactttattc ttacccctcc 420tacgtatatc ccgaattaat gcaagttgag ctcattggga ctgctcttgg gacatcattt 480<210>43<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>43aagctccggc cgccatggcg gccgcggaaa ttccatttcg agcggccgcc cgggcagata60cactcctatc tcaactctgt ttatttgatg agttctgtcc cgtaaatcat atttccctta 120caattaataa atgtcacttc atattttata ataaaccact cagtaaaagc aaaagcttgt 180cctgagaagt agagtgagtt ctttttcact ctgtgtctaa taatgttaag gtgggaaaaa 240aaaaagtgtg gcatagctac ctgcccatcc ccaaccctca gcaaagtaga atctcttttc 300tggtaatttt gggtttccgc tctgggctct ggcaagttga acaatcctag ccattgacaa 360tcgtgatagt tattattttc ccatttgctg tctttttgta tctaaagtct tcctattgta 420cctcggccgc gaccacgcta atcactagtg aattcgcggc cgcctgcagg tcgaccatat 480gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag 540cttgggcgta atcatggaca tagctgtttc ctgtgtgaaa ttgttatccg ctaacaattt 600cacacaacat acgagcacgg agcataaaga gtaagcctgg gggtgcctaa tgagtgagct 660<210>44<211>300<212>DNA<213>人属 人种(Homo sapiens)<400>44cattcgatgc tcccggcccg ccatggcggc ccgcgggaat tcgattagcg tgggtcgcgg60cccgaggtac agtcaaaaaa gagaaaatga aaaaaataca actaaaagga agaaacacaa 120cttcaaagat ttttcagtga tgagaatcca catttgtatt tcaagataat gtagtttaaa 180aaaaaaaaaa gaaaaaaact tgatgtaaat tcctcctttt cctctggctt aatgaatatc 240atttattcag tataaaatct ttatatgttc cacatgttaa gaataaatgt acctgcccgg 300<210>45<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>45gatgctccgg ccgcctggcg gccgcgggaa ttcgatttcg agcggccgcc cgggcagcca60ctggttctac aacagcccat aaaaacccat tggcctgaag cttatatctc aggcctatgc 120ccatcttata gtcttggaag acaaaaggct ggtagagaca gtcttcagtg gcttcagtga 180tgctctgtag aggccagggt gtcttgagtg ctgtaactcc caagcactgg gctagcctga 240cttctgtatc tccctaccac caccccctta aaaaaataag gtaacagcaa atctatagta 300aaaccatgtc tgcatagaac gtgttcaaat cctctgtttt cattaaatgt aaaagatgct 360gtctccatta agttgaatat ttggaattgg agaagccatt gattattatt ttgagtttct 420gtcatgtttt atagaaaagt aagatgctta ttcagaattt aagagtgaag gcaactgaaa 480tatgcattgt tgtagttatt tatatttcaa actaaaaata agcaaaaaaa aaaaaaaaaa 540aaaaaaaagt cctcagccgc gaccacgcta atcactagtg aattagcgga ccgcctgcag 600<210>46<211>360<212>DNA<213>人属人种(Homo sapiens)<400>46tcgatgctcc cggccgccat ggcggccgcg ggaattcgat tagcgtggtc gcggccgagg60tacaatttca tttctaatca tttttgcagt gatcattagt gaataaagaa cagatttaca 120actttatata gcagggcatc tgggttcaac acaaaactgt tacaaacgtt aggtaaatac 180atgtttcata agacactgct aacacttaaa ggaattaaat aaaattccaa atcttggaaa 240gcaaataaag acaagacaac taagaagcat tttccaccat ttactcttgt tatcaaaaat 300agttcagctc ttcttgcaaa acaaaaacaa aatagtacct gccccgggcg gccgctcgaa 360<210>47<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>47tccgttcgat gctcccggcc gccatggcgg cacgcgggaa ttcgatttcg agcggcccgc60ccgggcaggt actgtggatg tagagtataa aacttgaaaa atgcagatgt tgaaggaata 120ataggtatct tgtgctttaa tactttatgg caggattgta cctcggccgc gaccacgcta 180atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt 240ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat 300agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 360gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 420gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 480aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 540cgctgcgctc ggacgatcgg ctgcggcgag cggtatcaag ctaactcaaa ggcggtaata 600cgggtatcca cagaatcaag ggataacgca ggaaagaaca tgtgagcaaa aagccagcaa 660<210>48<211>600<212>DNA<213>人属人种(Homo sapiens)<400>48acagtccatt cgatgctccc ggccgccatg gcggccgcgg gaattcgatt agcgtggtcg60cggcccgagg tacttttttt tttttttttt ttttttttta aaagggcttt tattgagaca 120cttgtaggga ttctgcaaca tttccagttg ggaatcttta tttccaactc ttagggtggc 180taaacccttt tctgaatgcc aaaaggcaca ctctcaccac cttaatgagg aaacacactt 240atctgtgtct gttccgatat ccaactggga cctggacccc aagccccgtg gtgtcttgta 300cctgcccggg cggccgatcg aaatcactag tgaattcgcg gccgcctgca ggtcgaccat 360atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat 420agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 480ccacacaaca tacaagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 540taactacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 600<210>49<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>49cgcgacgctc ccggccgcca tggcggccgc gggaattcga tttcgagcgg ccgcccgggc60aggtccgggc aggtactttt tttttttttt tttttttttt ttaatacaaa tatattttat 120tgaaataaac aaaaatgcat acacagctca atgggtcact tggaacaaac ttgcttgact 180atattactga gcagaaacaa aacggaagca caaacacctt tctttttttg acatgtatct 240ggagataggt aggaagacac tacttggttt tttaaaaaac tgaccttccc ttaaggcctg 300gtcatagaag tgtaaacaat gtaaatgaat ccaccattac cagttgtcat atcatatcta 360tgtcacctgt gtattctgag attacacaca tacctgccaa tatacctggg aaaggttatt 420ttatcacagt tacacttgag ttcttggcag gcaggactga ggaagagtaa tttgaaagaa 480gttttacatc ctatttagaa gaaatcacta gtatttcctt aaataacagg ttacaataga 540aagatactgc ctggaagtta tcctttcact ttggctcatt tttagttttt ctttatgatt 600<210>50<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>50gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtactt60tttttttttt tttttttttt ggaacaaaac aggtatattt ctattagact acctgataca 120gaagatagtg tggtatggat ggatagtatg aaggacaaat aatacaaata tattttattg 180aaataaacaa aaatgcatac acagcccaat gggtcacttg gaacaaactt gcttgactat 240attactgagc agaaacaaaa cggaagcaca aacacctttc tttttttgac atgtatctgg 300agataggtag gaagacacta cttggttttt taaaaaactg accttccctt aaggcctggt 360catagaagtg taaacaatgt aaatgaatcc accattacca gttgtcatat catatctatg 420tcacctgtgt attctgagat tacacacata cctgccaata tacctgggaa aggctatttt 480atcacagtta cacttgagtt cttggcaggc aggactgagg aagagtaatt tgaaagaagt 540tttacatcct atttagaaga aatcactagt attttcttaa ataacagggt acaatagaaa 600gatctggctc ggagttatcc tttcactttg ggtcaatttt aggttttctt tatgaattac 660<210>51<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>51gctcccggcc gcctggcggc cgcgggaatt cgatttcgag cggccgcccg ggcaggtaca60tgctccaatt caaccccagg tggctctttt cctcccgttt tttcctattg gtttaatgct 120tagctatctc aaattctgta tgggccagga gtccctgaaa catatacaaa ttaggtcccc 180tttgtgacca acgtgctgtt aggcagctaa tctatttatc atctatttaa tgttcatcta 240gatcaatcag tcttctcaat cttgacatgc attttgggct gcataattct ttgttgatgg 300ggactgccta ttccctatag aattaacccc tggattctac ttatgtcagc agcactccct 360ccaattgtga caactacaat gtcttcaaac attgccagaa gtcccctgtg gggcaaaact 420gattgaaaac tggtactgta gattcaggca ggcccaccac tacccctatt caaattttct 480gggaaaggat ggcttggtgt catcctggga ccattaccaa acagaaatct ctacccagtt 540tcttaccttt atcctgtgct tgatcagcaa cgtccactcg aattcttctg gtacctagag 600actgagagca tagggaccat attaaccaac cttttgcccc attatgcaca aatcccttga 660<210>52<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>52ccatggcggc cgcgggaatt cgattcggcc gcccgggcag gtacatatgc tgccgttatc60attttaccgg caaaccacct gccatgcaat gcattgacag cagcaatagc tgcagcaatt 120gatgggcact tcacatacac attgccctga gctgaatttt tgtcaacata aatatgaata 180actcctccat gtttattaca ttcttcaatc acatcatcct taatctcggt atcccatcca 240acttcttctt ctgtttgagg gttaaacatg ttagagagtt ggaaacattg tgttgcaagt 300ggctgaacag aggcagctgc agctaaagct gaagcttcag tctgctggga aagtcttgtt 360tgcaaatctg ccacagcacc aaatgccaaa gagccactca tctgtagagc ttgctgtgct 420gctggcggaa tcagcaaacc tgtacctcgg ccgcgaccac gctaatcact agtgaattcg 480cgggcgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag 540tattctatag tgtcacctaa atagcttggc gtaaatcatg ggcatagctg cttcctgcgt 600gaaaatggtt atcccgtcac caatttccac accaacatac gagcccggga gccttaaagt 660<210>53<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>53gctcccggcc gccatggcgg ccgcgggaat tcgatttcga gcggccgccc gggcaggtac60tttttttttt tttttttttt ttttttggaa caaaacaggt atatttctat tagactacct 120gatacagaag atagtgtggt atggatggat agtatgaagg acaaataata caaatatatt 180ttattgaaat aaacaaaaat gcatacacag ctcaatgggt cacttggaac aaacttgctt 240gactatatta ctgagcagaa acaaaacggc agcacaaaca cctttctttt tttgacatgt 300atctggagat aggtaggaag acactacttg gttttttaaa aaactgacct tcccttaagg 360cctggtcata gaagtgtaaa caatgtaaat gaatccacca ttaccaggtg tcatatcata 420tctatgtcac ctgtgtattc tgagattaca cacatacctg ccaatatacc tgggaaaggc 480tattttatca cagttacact tgagtttttg gcaggcagga ctgaggaaca gtaatttgaa 540agaaagtttt acatcctatt tacaagaaat cactagtatt tccttaaata accagggtac 600aatcgcaaag atactggccg gaaggttatc ccttcacttt gggtcatttt aaggttttct 660<210>54<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>54gctcccggcc gcctggcggc cgcgggaatt cgattagcgt ggtcgcggcc gaggtactca60ttttttacct tgtagggagg gtgggcagtg gggttacctg accttatttg agggtatggg 120ctttcttttt ttatttcatc acttgttatc tcaaagagac tcggagccag tgatcctttt 180atcctgctac agtctttagg gagctaaaaa aaaaaaaaaa aaagcagggg ctgccaaaac 240tcttgatttc atatttcctt ctctaaatat atatgtatcc tgtttttttg gataaaattt 300taccaagaat ccaaaaaaaa aaaaacccta gaatttaatc aacaagatca gtctacaggt 360cacagtggat ttcttttcaa actgacaatg tttaggtttt aagcaaataa agttccagtt 420aatgtgaaac tcagtcacaa agagttgaga tttttccttt atgaaataga attgacattc 480ttttatgcta taaatgtgca ttcaggtccc attaaccatg ctctgctttt atttggggat 540agaacatttt ctttttcata tcccgatctt cccacttctt catagaaatg ctgataagaa 600<210>55<211>420<212>DNA<213>人属 人种(Homo sapiens)<400>55cgcgacgctc ccggccgcct ggcggccgcg ggaattcgat tagcgtggtc gcggccgagg60tacttttttt tttttttttt tttttggaac aaaacaggta tatttctatt agactacctg 120atacagaaga tagtgtggta tggatggata gtatgaagga caaataatac aaatatattt 180tattgaaata aacaaaaatg catacacagc ccaatgggtc acttggaaca aacttgcttg 240actatattac tgagcagaaa caaaacggaa gcacaaacac ctttcttttt ttgacatgta 300tctggagata ggtaggaaga cactacttgg ttttttaaaa aactgacctt cccttaaggc 360ctggtcatag aagtgtaaac aatgtaaatg aatccaccat taccaggtgt catatcatat 420<210>56<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>56cgcgcgcatg ctcccggccg ccatggcggc cgcgggaatt cgattcgagc ggccgcccgg60gcaggtacgc ggggttgctg gtgacagcaa aaatgaccca ccaatggaag cagctggctt 120cactgctcag gtgattatcc tgaaccatcc aggccaaata agcgccggct atgcccctgt 180attggattgc cacacggctc acattgcatg caagtttgct gagctgaagg aaaagattga 240tcgccgttct ggtaaaaagc tggaagatgg ccctaaattc ttgaagtctg gtgatgctgc 300cattgttgat atggttcctg gcaagcccat gtgtgttgag agcttctcag actatccacc 360tttgggtcgc tttgctgttc gtgatatgag acagacagtt gcggtgggtg tcatcaaagc 420agtggacaag aaggctgctg gagctggcaa ggtcaccaag tctgcccaga aagctcagaa 480ggctaaatga atattatccc taatacctgc caccccactc ttaatcagtg gtggaagaac 540ggtctcagaa ctgtttgttt caattggcca tttaagttta gtagtaaaag actggttaat 600gataacaatg catcgtaaaa ccttcagaag gaaaggagaa agtttttgtg gaccactttg 660<210>57<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>57cgcgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc60cgaggtacca gctctacagc aagaaattac caagaaaaat aaataaagaa ttaagaaaaa 120tactgtcaga aaataccata cttagtgtgc aggaagaggc aaaagaaaga aaaggaaata 180ccataggaat aggccaactt ggaggcaaga cctgccaagg tgaaataaat tatctagtcc 240tatctagtta atcactgagc attacctcaa gaacaggagg cagctagagg agaaaaactg 300tattatcaaa ccatactatc gctatgtcag agcacctccc ctattgaaag aacctatttc 360aattaagtaa ctttttttga agctgaatca gctaacttaa cttttaagaa atatgttgac 420atttttctta aaacaaggca cctctgtagg tatgttaact aatcactaac agcggtgggg 480<210>58<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>58gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtacca60gctctacagc aagaaattac caagaaaaat aaataaagaa ttaagaaaaa tactgtcaga 120aaataccata cttagtgtgc aggaagaggc aaaagaaaga aaaggaaata ccataggaat 180aggccaactt ggaggcaaga cctgccaagg tgaaataaat tatctagtcc tatctagtta 240atcactgagc attacctcaa gaacaggagg cagctagagg agaaaaactg tattatcaaa 300ccatactatc gctatgtcag agcacctccc ctattgaaag aacctatttc aattaagtaa 360ctttttttga agctgaatca gctaacttaa cttttaagaa atatgttgac atttttctta 420aaacaaggca cctctgtagg tatgttaact aatcactaac agcggtgggg ggagtgggaa 480<210>59<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>59tcgcgttcac gctcccggcc gcctggcggc cgcgggaatt cgatttcgag cggccgcccg60ggcaggcact tttttttttt tttttttttt tttttttaaa tgttcagtct tttatttaaa 120aactataaac agtcaccaaa gtaaataaag ccattctata acataaactg ttaggtctat 180attttttact gcacatccta aggacacagc agaaatggtg gttgggaggc cttccacatt 240tttggatgct aatagaacag gcaataggca gttataaatg gatacatttc acgctggggg 300aaaaaagaca atttaaggaa gtgagcagtt tctgagcagg aatgtggtac ctcggccgcg 360accacgctaa tcactagtga attcgcggcc gcctgcaggt cgaccatctg ggagagctcc 420caacgcgttg gatgcatagc ttgagtattc tatagcgtca cctaaatagc ttggcgtaat 480catggccata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 540gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtggcgcta actcacatt600<210>60<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>60cgcgcatgct cccggccgcc atggcggccg cgggaattcg atttcgagcg gccgcccggg60caggtactgg taagaaagga agtaaagaga agaaagaatg cattaacaga ataaaaacaa 120agtaaaacaa acttttctag gaatttggaa atgacaacag tgccatagtg acatccttaa 180aatgtctgat tcaagtatac tcttagaggg atagaaaact actcctttct caataatcaa 240aatagagttc actttgtcta cattttgttt aaaaatagga taaccaggaa tctatggctc 300ctcccaaaaa tatagtgccc ttccagatat gggcaaaaat tctttaaaag ggaataaatc 360cgaagtaaat ccaacaggga atctgcttgc aatagagaag caacacacat ctgggaatca 420accccagata ttcatggtga gtcccctccg tctgcattct aatgacatgt gtacctcggc 480cgcgaccacg ctaatcacta gtgaattcgc ggccgctgca ggtcgaccat atgggagagc 540tcccaacgcg ttggatgcat agcttgagta ttctatagtg gcacctaaat aagcttggcg 600<210>61<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>61cctgcgcgac gctcccggcc gccatggcgg ccgcgggaat tcgatttccg gcggcccgcc60cgccgggccc gctcacgttt cttgcagcct ttctttaact attgcttacc ctcccgggga 120agtccccaca gctggctgtg agctgacccg tcaacatgga gatcttacca ggagcccacc 180tacgggacac atgcctggac ataaaatgac tgtgccacta ttgggatgga ccatctaaag 240gcttctcttg ggcagtggcc gacccgtgac ctttatccgg agggccacca tacccatcct 300gcccgtatcc acgtgcctcc atagacgcga cactgtcggc ctgcatatcc tcgttgaaaa 360cctgcaactg gaaagacctg ttcacttaca ttttatgcac tggcccattg tcttacttac 420cgctagagat attgccattt tccgcacagg gcaacaggtc ttttaaatgc ataagcccat 480acttccaatt ccgttttgtt ctaaagcaca ctgcaaacgg gggatgctca tttaagtacc 540tcggccctac acccccctaa tcactagtga attcgcgggc gcctgcccgg gcgccctccc 600<210>62<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>62ttcgccccct cccggccgcc atggcggccg cgggaattcg attgaccggg cccccggcca60ggtacttttt tttgttcttt tttttttctc aattgtgaac aactgcacaa tcctaactca 120cccaccacct gaagcctctg ccccggtgac cattggcagc cagggacagg ccttgcacca 180ctctttcccc tgtcacacgc aacgttcgac tactgcttag tagctctgaa taaatacttt 240tctctgtcag taaagtcccc gccttaataa taaatattaa ttctatggca cactttagac 300acacggcact gcctgtcctc atggcaccat ccataaaccg aaccacccgg gcccattcgc 360gcctccccta ttgagtaaca tactccctat ctcaactctg ctatcgctgc acctgccctc 420actataatta gaactgcggg caacatggaa aggatactgc tcatacttgg tgccacaatg 480agcttattta tttataaccc cttgaatgtg ggccccgctc gcaggaacat tagactcaat 540aaagccttgg ctgctcaact ttgaaaataa actatcaagc atgatacata cgtaatcctc 600atgaaaatta ccagagtcag acaaaacctg aaacgtttct agcacaatcc ttggcactta 660<210>63<211>278<212>DNA<213>人属 人种(Homo sapiens)<400>63actataatta gaactgcggg caacatggaa aggatactgc tcatacttgg tgccacaatg60agcttattta tttataaccc cttgaatgtg ggccccgctc gcaggaacat tagactcaat 120aaagccttgg ctgctcaact ttgaaaataa actatcaagc atgatacata cgtaatcctc 180atgaaaatta ccagagtcag acaaaacctg aaacgtttct agcacaatcc ttggcactta 240gcgggcatta aataaatgtt tatagttaaa gaaaatat 278<210>64<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>64ccttcgtcgc gctcccggcc gccatggcgg ccgcgggaat tcgatttcga gcggccgccc60gggcaggtac agtcatgccc tgagtgctga aatgtggaca ctgacctatc cacaaggagc 120cctcaggcac attaatacgt tggcacatca tgtttcccaa aagcacatgc atgtgtgtgt 180ctatttcaac tcttaacaat tgctcaacac cactctaaac cattttcgct tccataaagc 240taattatcgc agatcttccc aaaagagaag ttcacatttc cttgatcaca gctatactct 300cattacctag gaagaacccc gggacctctg gggctgtttg cccccgcaga gcactgctca 360ttcctgcaga ttcatttggg acttgggttt caagttcaat gattcttcat tgagatacaa 420tgaacttatc taactgtatt cttgttagta cctcggccgc gaccacgcta atcactagtg 480aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag 540cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc 600tgtgtgaaat tggtatccgc tcacaattcc acacaacata cgagccggaa agcataaagt 660<210>65<211>360<212>DNA<213>人属 人种(Homo sapiens)<400>65atatcccccc ggccgtcatg gcggccgcgg gaattcgatt agcgcggtcg cggccgaggt60accctctgcc atcttgccga gggagtctcc ttttagaaaa caatcaaagg gttattgcat 120gagtctggat gaatcccact ctcagctgtc cacgggcccg accacctcat ctagccccct 180tttcggcagg gagaacctgg ctcccaagtt ctcctccttc acttcgttac aaaccaaggg 240gaagagccca ccgtgagaac gcgccatctg caagctgtct ccctttctcc atccttggtg 300aaaccctttg cgcagaaggc aacgtggaag ctgatgtttt tactcatctc gctgtttgaa 360<210>66<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>66ctcccggccg ccatggcggc cgcgggaatt cgattgagcg gccgcccggg caggtacttt60tttttgtttt tttttttttt ttaattttga acaactgaag aatactaaat cagagagtag 120ctgaagcctc tgccccggtg accattgtca gccagggaca ggccttgaac aactcttttc 180cctgttagat gtaaagttag actagtgctt agtagctctg aataaatact tttctctgtc 240agtaaagtgt ctgcattaat aataaatatt aattatatgg gacagttttg acagttggaa 300ctgcctgtct tcatggaaaa attcagaaat tgtttaaatt gggaacattt ttgccttctt 360tattgagtag tatagttcct atattaactt tgttattgtt gtagttgacc tcactttaat 420tagaactgtg gtcagtatgg aaaggatact catcatactt gagccgaaat gagattattt 480atttataagc attgaatgtg ggccaagctg gcaggaacat tagagtaaat aaagccttgg 540ctgctaaact ttgaaaataa actatcaagc atgatacata cgtaatcctc atgaaaatta 600ccagagtaag acaaaatttg aaagtttcta gcacaattct tggcaattag tgggcattaa 660<210>67<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>67aagcttcgcg ccctcccggc cgcctggcgg ccgcgggaat tcgattagcg tggtcgcggc60cgaggtactt tctttttttt tttttttttt aagccagttt ataaagtgga agtattagga 120gattcataaa tcttctatat tgagaattgg ctatgttaat aaatattaca acatcattaa 180ggttttagct aagtttgatt catgctgtct gttaaatcaa aactgatcta aatcagaatt 240attaaatgtg aggagctttt ttaatacagg aaaagaaaca tgtcatccac ttgagttaat 300agttttccta cgttgatgac agccctcatg agtagcatcc acatttttaa aatttcaaat 360tggtttttct actagtagat tgtgtttcta gagaaagata caaggcatag gtgattgttt 420aggattttcc tctagccttt gccattacct ttttggggat gaggttcaca gtagactttg 480agtgaccgtc ccaccgtgaa gtgaattctc tgagctggtg gtgtggtgct ggaaggaagg 540ttatttttgg agccactctc tccccttaag gatatttccc aagggcctgc ttcaattctt 600tgatgacttt agaggtgaaa aaatattttt atggagatga tgcagaaaac ctccaattca 660<210>68<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>68ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattag cgtggtcgcg60gccgaggtac tttcttcttt tttttttttt ttactatcag ttattagttc ctgttttctc 120aagaattaaa tttgtttact ttaggttctt gctcagaact ctggttttga ccttcaggaa 180acattagtta aaattcaagc agaacattca gaatcaggtc agcttgtggg tgtggacctg 240aacacaggta agagaatgca actgttgtag agggaaaact agatgtaata cgtgtgagtt 300gaagccagga aagattaaaa atgttttgct ttgcaatttg acaccagtgc tgttagggaa 360ctctgggttc taaaatggga taaaatagga tattaataaa aaatggaatg actaggtata 420attaagggac gagtaacatt gagtaatttg gggatacaat tttggaaatt gttgagtggt 480<210>69<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>69gtcgcatgct ccggccgcca tggcggccgc gggaattcga ttagcgtggt cgcggccgag60gtactttttc tttttctttt tttttttttt aagtattgtt aacaatcctt tggaagtcac 120tactggtctt tgtgtgctgc tttttaataa ttgagttatt ttgagcttgc caagtaggat 180ctattgcctg gactaaaatt tatttcctaa tcttctgatg accaagaaag gaaaaattaa 240gtttgcagat gtgagatgaa atatagccag tgaatatgca tactgattct gaatgaaagg 300aattaacttt tcagtcaaga aacagtctgc atgcagtaaa ttgaattttt cctgcaactg 360ggaatgattt gtttaattct tctttgaaca ctgccctttc tccagttaag aacactaatg 420atttgctaat atttttttaa aagaaatcct ggtttttttt aatttagttt aagccccaaa 480acttcccccc ttaatttttg taatgcctaa aaagaaaaac tggcgaaaaa gggggaattg 540<210>70<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>70caatggggcc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgatttcga60gcggccgccc gggcaggtac taatatccct taatggcaga atgtgataat catggaatta 120attattgcta aagtagtttt caaataaaaa aagaaaaaag aaaacaaaac aaattaaact 180tgacacaatc tgaccaaaca cgtgtcaatt tacaatttca aggttatttt acaaaatacc 240tacatttacg caataggctc ccggcagcat ttccagacaa atgttctttt aatttatcct 300gacatgctat aaatgaataa attacactat ttaaaaatta tcatcagtaa gtttcctttc 360tcatgggtca gagaaaaaga aatgagcgtg caaagaagat tgagaggaag cgcacgcaca 420cagattactt aaaagaagca ggaggggctg ggggagggtc aacaatcact ggaacctcac 480acagtggtga tgccctccaa agggtgggaa gtcaactggc ttattagcca tttcaaaagt 540atttttaaaa aaaaaatggt ttggtagggg gaaacccttt aatggctttc atttttgatt 600<210>71<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>71caatggggcc gacgtcgcat gctccggccg ccatggcggc cgcgggaatt cgattagcgt60ggtcgcggcc gaggtacaaa tgcttgggac ctccccttaa tcaacagata aaatacataa 120cttacattta aggggagtgg atacctcaaa gtgcttaagt aaaaatttgt tctctttaat 180agtttgaact gtcccttgtt agtcgcgtct aaaacattga cctgaataat gagaaatcac 240aagctaatgt tttacttatt ccttatatgg taagagacta ccttttaaag caccattttc 300catttgggca tttcaccaag gtctaatgtc cctggaatta gtttttttgt tttgcctttt 360gaatatcgat aggtgttcat ctgaattaaa ctacgtttaa gtttgatggg cttcctgcct 420ccaaattggc aacaagtgta cctgcccggg gcggccgctc gaaatcacta attgaattcc 480gccggccggc tgcagggtca accatatggg gagagctccc aacgccttgg gattgcatta 540<210>72<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>72gggccgacgt cgcatgctcc cggccgccat gggcgggccg cgggaattcg attagcgtgg60tcgcggccga ggtacactcc tatctcaact ctgtttattt gatgagttct gtcccgtaaa 120tcatatttcc cttacaatta ataaatgtca cttcatattt tataataaac cactcagtaa 180aagcaaaagc ttgtcctgag aagtagagtg agttcttttt cactctgtgt ctaataatgt 240taaggtggga aaaaaaaaag tgtggcatag ctacctgccc atccccaacc ctcagcaaag 300tagaatctct tttctggtaa ttttggggtt tccgctctgg gctctggcaa gttgaacaat 360cctagccatt gacaatcgtg atagttatta ttttcccatt tgctggtctt tttggtatct 420aaagtcttcc tattgtactg cccgggcggc cgctcgaaat cactagttga attcgcggcc 480<210>73<211>600<212>DNA<213>人属人种(Homo sapiens)<400>73gccgacgtcg catgctccgg ccgccatggc ggccgcggga attcgattag cgtggtcgcg60gccgaggtac tggcaagaaa ggaagtaaag agaagaaaga atgcattaac cgaataaaaa 120caaggtaaaa caaacttttc taggaatttg gaaatgacaa cagtgccata gtgacatcct 180taaaatgtct gattcaagta tactcttaga gggatagaaa actactcctt tctcaataat 240caaaatagag ttcactttgt ctacattttg tttaaaaata ggataaccag gaatctatgg 300gctcctccca aaaatatagt gcccttccag atatgggcaa aaattcttta aaagggaata 360aatccgaagt aaatccaaca gggaatctgc ttgcaataga gaagcaacac acatctgggg 420aatcaacccc agatattcat gggtgagtcc cctccggtct gcattctaat gacattgtgt 480tactgcccgg ggcgggccgc tccgaaatca ctaattgaat ttcgcgggcc gcctgcaagg 540tcgaccatat tgggaaaact gcccaacgcg ttggattgca ttacctggag tattctatag 600<210>74<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>74gtcgatgctc cgggcggcat ggcggcgcgg gaattcgatt agcgtggtcg cggccgaggt60acactcctat ctcaactctg tttatttgat gagttctgtc ccgtaaatca tatttccctt 120acaattaata aatgtcactt catattttat aataaaccac tcagtaaaag caaaagcttg 180tcctgagaag tagagtgagt tctttttcac tctgtgtcta ataatgttaa ggtgggaaaa 240aaaaaagtgt ggcatagcta cctgcccatc cccaaccctc agcaaagtag aatctctttt 300ctggtaattt tgggttccgc tctgggctct ggcaagttga acaatcctac cattgacaat 360cgtgatagtt attattttcc catttggctg tctttttggt atctaaagtc ttcctattgt 420actgccgggg cggccgctcg aaatcactag ttgaattccg cggcccgcct gcaaggtcga 480<210>75<211>420<212>DNA<213>人属 人种(Homo sapiens)<400>75ccgacgtcgc atgctccggg cgccatggcg gccgcgggaa ttcgatttcg agcggccgcc60cgggcaggta cacatgtcat tagaatgcag acggagggga ctcaccatga atatctgggg 120ttgattccca gatgtgtgtt gcttctctat tgcaagcaga ttccctgttg gatttacttc 180ggatttattc ccttttaaag aatttttgcc catatctgga agggcactat atttttggga 240ggagccatag attcctggtt atcctatttt taaacaaaat gtagacaaag tgaactctat 300tttgattatt gagaaaggag tagttttcta tccctctaag agtatacttg aatcagacat 360tttaaggatt gtcactatgg cactgttgtc atttccaaac tcctagaaaa gtttggttta 420<210>76<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>76cccactcggg ccgacgtcgc atgctcccgg ccgccatggc gggcgcggga attcgattag60cgtggtcgcg gccgaggtac atttattctt aacatgtgga acatataaag attttatact 120gaataaatga tattcattaa gccagaggaa gaggaggaat ttacatcaag tttttttctt 180ttttttttgt taaactacat tatcttgaaa tacaaatgtg gattctcatc actgaaaaat 240ctttgaagtt gtgtttcttc cttttagttg tattttttca ttttctcttt tttgactgta 300cctgcccggg cggccgctcg aaatcactag tgaattcgcg gccgcctgca ggtcgaccat 360atgggagagc tccaacgcgt tggatgcata cttgagtatt ctatagtgtc acctaaatag 420cttggcgtaa tcatggtcat actgtttcct gtgtgaaatt gttatcccgc cacaattccc 480acaacataca gccggaagca taagtgtaag cctggggtgc taatgagtga ctaaccccat 540<210>77<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>77tggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga ttagcgtggt60cgcggccgag gtactttttt ctttttcttt tttttttttt taagtattgt taacaatcct 120ttggaagtca ctactggtct ttgtgtgctg ctttttaata attgagttat tttgagcttg 180ccaagtagga tctattgcct ggactaaaat ttatttccta atcttctgat gaccaagaaa 240ggaaaaatta agtttgcaga tgtgagatga aatatagcca gtgaatatgc atactgattc 300tgaatgaaag gaattaactt ttcagtcaag aagcagtctg catgcagtaa attgaatttt 360cctgcaactg ggaatgattt ggtttaattc ttctttgaac actgcccttt cccagtagaa 420cactaatgat ttgctaatat tttttaaaga aacctggttt tttaatttag ttaagctcaa 480<210>78<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>78gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc60cgaggtactg ttcccgttgg ccgagtgggg actggtgttc tcaaacccgg tatggtggtc 120acctttgctc cagtcaacgt tacaacggaa gtaaaatctg tcgaaatgca ccatgaagct 180ttgagtgaag ctcttcctgg ggacaatgtg ggcttcaatg tcaagaatgt gtctgtcaag 240gatgttcgtc gtggcaacgt tgctggtgac agcaaaaatg acccaccaat ggaagcagct 300ggcttcactg ctcaggtgat tatcctgaac catccaggcc aaataagcgc cggctatgcc 360cctgtattgg attgccacac ggctcacatt gcatgcaagt ttgctgagct gaaggaaaag 420attgatcgcc gttctggtaa aaagctggaa gatggcccta aattcttgaa gtctggtgat 480gctgccattg ttgatatggt tcctggcaaa cccatgtgtg ttgagagctt ctcagactat 540<210>79<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>79tgatgggccg acgtcgcatg ctccggccgc catggcggcc gcgggaattc gatttcgagc60ggccgcccgg gcaggtacgc ggggcctcag caggtgtgaa gcgtgtgctt tagtttcgtg 120ggaggcctgg catccccgag agggagggga aaggtaacca ctcctttgtg gaggtcgcca 180gggtcattg tcgtggattt gcacagtcgg ctgggcggtg caatggcgga aagaaaagga240acagccaaag tggacttttt gaagaagatt gagaaagaaa tccaacagaa atgggatac300gagagagtgt ttgaggtcaa tgcatctaat ttagagaaac agaccagcaa gggcaagta360tttttaacct tcccatatcc atatatgaat ggacgccttc atttgggaca cacgttttct 420ttatccaaat gtgaaccttg ctggtagggt acccgggatg gcacatgcag cgcaagtagg 480<210>80<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>80cattcaatgc tcccgccgcc tggcggccgc gggaattcca tttcgagcgg tcgcccgggc60agcgtacgag atctccgacc agtctggggg cgctggcggc ctgcgcagcc acctcaagat 120cacagattct gctggccata ttctctactc caaagaggat gcaaccaagg ggaaatttgc 180ctttaccact gaagattatg acatgtttga agtgtgtttt gagagcaagg gaacagggcg 240gatacctgac caactcgtga tcctagacac gaagcatgga gtggaggcga aaaattacga 300agagattgca aaagttgaga agctcaaacc attagaggta gagctgcgac gcctagaaga 360cctttcagaa tctattgtta atgattttgc ctacatgaag aagagagaag aggagatgcg 420tgataccaac gagtcaacaa acactcgggt cctatacttc agcatctttt caatgttctg 480tctcattgga ctagctacct ggcaggtctt ctacctgcga cgcttcttca aggtcaagaa 540attgattgag taatgaatga tgcatattct tcttccacct tggacctcgg ccgcgaccac 600<210>81<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>81atgctcccgg ccgccatggc ggccgcggga attcgatttc gagcggcccg cccgggcagg 60tacttttttt tttttttttt ttttttttgg ctctagaggg ggtagagggg gtgctatagg 120gtaaataccg gccctatttc aaagattttt aggggaatta attctaggac gatgggcatg 180aaactgtggt ttgctccaca gatttcagag cattgaccgt agtatacccc cggtcgtgta 240gcggtgaaag tggtttggtt taacgtccgg gaattgcatc tgtttttaag cctagtgtgg 300ggacagctca tgagtgcaag acgtcttgtg atgtaattat tataccaatg ggggtttcaa 360tcgggagtac ctcggccgcg accacgctaa tcactagtga attcgcggcc gcctgcaggt 420cgaccatatg ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca 480cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaaat ggtatcccct 540tacaatttcc acaacatacc agcccgaagc ataaagtgta aagcctgggg tgcctaatga 600<210>82<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>82ttccattaat ctccgccgcc atggcggccg cgggaattcg attagcgtgg tcgcggcccg60aggtacaagg ccattgcttt ccggccattt aaaggggagg tcgtggatgc tgttgtcact 120caggtcaaca aggttggact cttcacagaa attgggccca tgtcttgctt catctctcga 180cattccatcc cttcagagat ggagtttgat cctaactcca acccaccatg ttacaagaca 240atggatgagg atattgtgat tcagcaggac gatgagatcc gcttaaagat tgcggggacc 300cgtgtggaca agaatgacat ttttgctatt ggctccctga tggacgatta cttggggctt 360gtaagctgag cctggtggcc tcctaccctt ggtcctactc taggaagtgt gattgtcaca 420cttatcatgt tgccagaggt ccagtctggc tgctgttgtg gaggcaagga aggccactta 480<210>83<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>83gtctcattgt atgctcccgc cgccatggcg gccgcgggaa ttcgatttcg agcggccgcc60cgggcaggca ccaagaatgc cctgtcagag gacttgtgct ctggtctggt agcaaatgcc 120cccagcgctg actccatatc cccggctgtc agattctctt ctacatctac actatagtcc 180agaccctcaa gtagctcctt catccctgtg atgtcaaagt cagctccatt cctcggaggc 240agatggtcaa actctgtatt gcatatgatg agagccaggc gtgtgcggtt gtttctctcc 300tttattggat agatctcttc agctctttct ctacatagtc tcaggaattc ttcatgagga 360caaagcttga gggcatctgt agattctctg actcaggtgg tccagcctcc atattcggat 420gagctttttt attgggggat atttggtcta tgttaaaaaa ggtttgaaga agcatttgtc 480ctgccatacc gttgcttctc ttgcatagag tctgccatga ccccgaactt tgtcttcagt 540tttagcatct taatatttct ttttttcctc tttcttcagt tcattacctc ggccgcgacc 600acgctaaatt actagtgaat tcccgtccgc tgcaggtcga ccatatggag agctcccacg 660<210>84<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>84gtgccctcgt cttgctcccg gccgccatgg ccgcgggatt caatcatcag ccgttatgtg60gcttcggagg aaggcaaatt gtagcacttg gcaaaatcac aacgccagta accttcgact 120ttgttcataa tacaaggact gaacaggttg tgtttgacat tgttgacatg gagtacccct 180actatgcaat cattggtcgt gggacactta atgctttcga agcaatactt catccagcat 240atctatgcat gaagatacct tcggaacaag ggcccattgt tgttcatggc agtcaagaag 300ctgccaggaa ggccggaggg aaatgaacca gagaatcact agtgcggccg cctgcaggtc 360gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 420ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 480acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 540<210>85<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>85atgctcccgg ccgcctgcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtacgc60ggggggacaa catggcggcc ttcatgctgg gctcgctgct gcggacgttc aagcagatgg 120ttccttcatc agcttcaggc caagttcgaa gtcactatgt agactggaga atgtggcgcg 180atgtgaagag acgaaaaatg gcctatgaat acgcagatga gaggctacgt attaattcac 240tcaggaagaa taccattttg ccaaaaattc ttcaggatgt ggctgatgaa gaaattgctg 300ccctcccccg ggatagctgt cctgttagaa tcagaaatcg gtgtgttatg acgtcccgtc 360cgcgtggtgt gaagcggcgc tggaggctta gtcgtatagt cttccgtcac ttagctgacc 420atgggcaact ttctgggatc cagcgagcga catggtaaat gagctccaga acctattgag 480cttgcaggga agccaagctt gcagttccag caagcaaaga ttttttttaa tagaccaaac 540cctaatctct acaggggccc agtacctgtc ccggccgggc gttcgaaatc actagtgaa600<210>86<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>86atgctcccgg ccgcctgcgg ccgcgggaat tcgattagcg tggtcgcggc cgaggtactt60tttttttttt tttttttagt tcatgtcttt tattaactca tacagttact tgtcttctgg 120tttgttgaaa cagtaagtca gacaacattt gccacaataa tgtctgtcaa agtgacttgc 180cataaacacc ccagcaccac attcaccaga agggcactct cgacgaaggc gactaatttt 240gccattctca tccaccttat aatatttcag gacagccagc ttaaccttct ttctcttggg 300cttattcttc ttgggagtgg tgtaagactt cttcttcctt ttcttagcac caccacgaag 360tctcaacaca agatgaagag tagactcctt ttgaatattg tagtcagaca aagtacctgc 420ccgggcaggt acgactctgt gaacagctct ccacagttat accaaactca catgtttatt 480acagaagagg actggctctg aaaaaaatta tttcacagtg catcgcaaga gatttcacaa 540gacctgattg gtattaatga agatcgtaaa accccaaatg gacttatttt gagtcacttg 600<210>87<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>87tcccaattgg gccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgatta60gcgtggtcgc ggccgaggta catgaaagtg taataataaa atgtctatgt atctttagtt 120acattcaaat ttgtaacttt ataaacatgt tttatgcttg aggaaatttt taaggtggta 180gtataaatgg aaactttttg aagtagacca gatatgggct acttgtgact agacttttaa 240actttgctct ttcaagcaga agcctggttt ctgggagaac actgcacagc gatttctttc 300ccaggattta cacaacttta aagggaagat aaatgaacat cagatttcta ggtatagaac 360tattgttatt gaaaggaaaa ggaaaactgg tgtttgtttc ttagactcat gaaataaaaa 420attatgaagg caatgaaaaa taaattgaaa attaaagtca gatgagaata ggaattatac 480tttgccactt ctgcattatt taaaaacata cgttattgta ccggcccggg cgggccgccc 540<210>88<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>88tgggccgacg tcgcatgctc cggccgccat ggcggccgcg ggaattcgat ttcgagcggc60cgcccgggca ggtactcccg attgaaaccc ccattcgtat aataattaca tcacaagacg 120tcttgcactc atgagctgtc cccacactag gcttaaaaac agatgcaatt cccggacgtc 180taaaccaaac cactttcacc gctacacgac cgggggtata ctacggtcaa tgctctgaaa 240tctgtggagc aaaccacagt ttcatgccca tcgtcctaga attaattccc ctaaaaatct 300ttgaaatagg gcccgtattt accctatagc accccctcta ccccctctag agacaagcgc 360acccccgact caaccgaatt ttgttacctc gggccgcgac cacgctaatc acttagtgaa 420tttcgcgggc ggctggcagg tcgaaccatt atggggagaa gcccccaacg cgttgggatt 480<210>89<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>89caatgggccg acgtcgcatg ctccggccgc catggcggcc gcgggaattc gattagcgtg60gtcgcggccg aggtacattc ctcattttac attcatttaa cctgccgatt atttaatttt 120tttattgtaa agtagttttt agcatttgct tttatttttt tactttgatg ccttttcaaa 180ttggcatgtc tttaaagtat ttttcttcct gattaaaaat gtgtgtgtat gtgtgtgtgt 240gtgtgtgtat atatatatat tttggtaaat cacattaatt ttaccaagtg aaaccaagcc 300atactgtttt tgagccaatt aagaaaattg ccatttttaa agtgtagcat ttcagggtaa 360agacccatga aatgggcttg atgtattcta gactactgaa agaaaaccac ttcaaagatt 420ttggtggaaa gtttaagtgg gtggtctgaa atgcaagaag gaaaggtgat tgggtagtgg 480agtttaaaga aaaagaagag ggaacagaga gtagtttggc tcaagtaaat gtctgggtgg 540gccaaacatt cccaagtggt gaaaggagat aggaaaaccc actgaggggt gtataagttg 600taaaagggca agggactgga ctattgcctg gttgggaacg gcaataatga ttggaaaaag 660<210>90<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>90tgatgggccg acgtccatgc tccggccgcc atggcggccg cgggaattcg atttcgagcg60gccgcccggg caggtaccgt attttctctg atccgtatac cagtaatagg gagtggggtc 120aatcccttcc cttttataac ctgccagtat ttcttcacta tcaaagatac gcattgagcc 180tcccacaatc tcaccaacat tgggcatcaa cacgtcgaca gattcagtaa gacgggaatc 240ctcaggacat cgctgcatgt agaaggactt gatctccaca ggaaatcgac acagcaagat 300tggttcatta atgggtgtct gtcatcagtc tctcaggagc ttctgggata tcttctccaa 360attcatagaa agttccatct tctttcttta catcatgttc ttttagccaa acgatacatc 420tgaatagtca tccgtttgaa aggccgtttg gggggctgaa gttcgggttg actcatgcac 480tagctcccgc aggtgattca atattcatct acaatcacaa caagtctcaa cggtcaggag 540gtcgcaaagt cagaaaggac agcaggtcca gtgatgtact cgccggacac ctatcagaat 600gattcgcggc gctgaggtca catttggaaa ggccagccgt ggtgctactg gatgtcataa 660<210>91<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>91ccaatgggcc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattagcg60tggtcgcggc cgaggtacag aagctggtct gatggcctta agagaacgta gaatgaaagt 120aacaaatgaa gacttcaaaa aatctaaaga aaatgttctt tataagaaac aggaaggcac 180ccctgagggg ctgtatctct aatgaaccat ggctgtcatc aggaaaatgg ttgggagatt 240tctcaatccc tgaaagggat gaggttgggg gagttgccca gaggaatccc tgttcccact 300gatttttatt agcaaaacat cctgtgtctt ttggagtacc tgccggggcg gccgctcgaa 360atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt 420ggatgcatac ttgagtattc tatagtggtc acctaaatac ttggcgtaat catggtcata 480ctgttcctgt gtgaaattgt atccgctcac aatccacaca acatacaacc gaagcttaag 540<210>92<211>660<212>DNA<213>人属 人种(Homo sapiens)<400>92tgggccgagt cgcatgctcc ggccgccatg gcggccgcgg gaattcgatt agcgtggtcg60cggccgaggt acataggccc tttcctcatc acgatctaat ctgcattctt ctgctgtctt 120aaagatcttc agggcactgt gcacataact cttagtgctt attttctctg gtttaacttc 180tgtcttctta ttaaggtctt ttagtgaaga actgaggtag agttctttag gaactgaagc 240cacagcaggc atgatgaatt atctttactt ttccactttc acaatggatg ctatatttcc 300ttacaagtgc ttctttccgt gacgccgggc gcgcttcact aacgccagcc ttctcgccaa 360atgcccgtct cccaagaact cgaggggaga attagcgttg ggctaccagg acggaagcca 420agccagctca accccccccg gcgtacctgg cccggggcgg gcgctccgaa atcactaag480tgaattcccg ggcccgcctt gcaaggtcga accatattgg ggaaaagctg cccaaacgcc 540ggttgggatt gcattaactt gggagttatt ccttattagt ggtcacccta aaattagctt 600gggggggtta atccatgggg ccattaacct gggttgccct gggggttgaa aattgggtta 660<210>93<211>600<212>DNA<213>人属 人种(Homo sapiens)<400>93agcacaatgg ggccgacgtc gcatgctccc ggccgccatg ggcggccgcg ggaattcgat60ttcgagcggc cgcccgggca ggtacttata ccccctaaat atataaaaca tttttaaaag 120aaaaaaagga agaaactatt catacatgca acaacttgga tggatttcaa gggaattatg 180ctgaatgaaa aaagatcagc ctcgtaagat tacattctgt atgattccat tcatacaaca 240ttcttgaaat gacaaaatta cagagatgga ggacagaaca gtggtagcca caggttgggg 300tgagggtata agaaagggat gtggctgcgg ctgtaaaagg gcagtgcaag gggatccatg 360tgacagaact gttctgtctc ttgtgatggg tggtcacatt gaatctacac attgttgata 420atattgcata gaattaaata cacatacacg aaaaaagttc aagcagttga gcacaaatat 480tttaattgtc taaaatgaca ttttctttaa gagttatcta cagtcaaagc tcacttttat 540gaggtgtcaa ttcatcacca ttttaagaga tataaatcat gaaaagattc cgccagaagc 600<210>94<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>94tgggccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga ttagcgtggt60cgcggccgag gtacacagta tgtataacaa tgcatactat ggtgtggagt taattccaat 120taccatattt tatatttatt ggtcacaaca gcatacattt tatgctccaa aatacatgga 180tctgacaaaa tggttacatt taatgttctt ttaaagaaag atgaactaaa tttaagaaga 240attggttttt cctaatatct cattttcaaa ttactgatac aaatttgcca gagaaacaat 300tacatgtttt acctaacatc aaataatctc cagtttctaa gacagatgca tttcttgttc 360aatttccaaa agtaaataaa ggctttctaa ctgaaaacat ttgcatccct agctctctaa 420agtaattaaa aagaaaatcc aaaaaatgac ctctaagctt ctgaacagcc acttagttac 480<210>95<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>95acgtccatgc tcccggccgc catggcggcc gcgggaattc gattagggtc gcggccgagg60tacttttttt tttttttttt tttttttttt aggtattgct tgcattaata aattaaagct 120ccatagggtc ttctcgtctt gctgtgtcat gcccgcctct tcacgggcag gtcaatttca 180ctggttaaaa gtaagagaca gctgaaccct cgtggagcca ttcatacagg tccctaatta 240aggaacaagt gattatgcta cctttgcacg gttagggtac aagaggagag tgaagaggaa 300gaggtcgatg aaacaggtgt agaagttaag gacatagaat tgggtcatgt cacaagcaaa 360tgtgtcgaga gcaaaggcag tccgagccct gaagaacaac agtaatgata ttgtaaatgc 420gattatggaa ttgacaatgt aaccatattg gaagcaactt tttttggtgt ccaaaaggag 480<210>96<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>96ccgacgtcgc atgctcccgg ccgccatggc gggccgcggg aattcgattt cgagcggccg60cccgggcagg tactcctcgg gtcaaaacgt cttctttctg atgacaggag caatattcta 120atttatatga cagggcatgg tggaaatggt ttcttaaaat ttcaagattc tgaagaaatt 180accaacatag aactcgcaga tgcttttgaa caaatgtggc agaaaagacg ctacaatgag 240ctactgttta ttattgatac ttgccaagga gcatccatgt atgaacgatt ttattctcct 300aacataatgg gctctagcta gtagtcaagt ggggagaaga ttcaatctcg catcaacctg 360atcctgcaat tgggagtcca tcttatggat agatacacat tttatggtct tggaatttct 420gggaagaaat taacccagct aagcccaaac taatatgaat ggaccttttt ccaggtatgt 480<210>97<211>360<212>DNA<213>人属 人种(Homo sapiens)<400>97ccgcctgcgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat ttcgagcggc60cgcccgggca ggtactaagg ttcaccaaga ggttgtcaga cagggtttgg ctgggccagc 120agagacttga caactccctc tacctaacca gctgcccaac tgtagaaact accaacccac 180cgaccaacag ggagagggaa caagcaccct caagggggtc aagttctaga ccccatgtaa 240taaaaggtgg tttcaaggcc agatgtacct cggacgcgac cacgctaatc actagtgaat 300tctcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt 360<210>98<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>98tcccccgcgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat ttcgagcggc60cgcccgggca ggtaccattg gtggccaatt gatttgatgg taagggaggg atcgttgacc 120tcgtctgtta tgtaaaggat gcgtagggat gggagggcga tgaggactag gatgatggcg 180ggcaggatag ttcagacggt ttctatttcc tgagcgtctg agatgttagt attagttagt 240tttgttgtga gtgttaggaa aagggcatac aggactaggg agcagataag gaaaatgatt 300atgagggcgt gatcatgaaa ggtgataagc tcttctatga taggggaagt agccgtcttg 360tagacctact tgcgctgcat gtgccatccc gcgtacctcg gccgcgacca cgctaatcac 420tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg 480catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg 540<210>99<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>99agcttgcatc caacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat60tcactagtga ttagcggccg tccgggcagg tacgcgggca gtcaaagcga actaccatac 120tcaattgatc caataacttg accaacggaa caagttaccc tagggataac agcgcaatcc 180tattctagag tccatatcaa caatagggtt tacgacctcg atgttggatc aggacatccc 240gatggtgcag ccgctattaa aggttcgttt gttcaacgat taaagtccta cgtgatctga 300gttcagaccg gagtaatcca ggtcggtttc tatctacttc aaattcctcc ctgtacctcg 360gccgcgacca cgctaatcga attcccgcgg ccgccatggc ggccgggagc atgcgacgtc 420gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcag ttttacaacg 480tcgtgactgg gaaaaccctg gcgttaccca acttaatagc cttgcagcac atcccccctt 540<210>100<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>100agcttgcatc caacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat60tcactagtga ttagcggccg tccgggcagg tacgcgggca gtcaaagcga actaccatac 120tcaattgatc caataacttg accaacggaa caagttaccc tagggataac agcgcaatcc 180tattctagag tccatatcaa caatagggtt tacgacctcg atgttggatc aggacatccc 240gatggtgcag ccgctattaa aggttcgttt gttcaacgat taaagtccta cgtgatctga 300gttcagaccg gagtaatcca ggtcggtttc tatctacttc aaattcctcc ctgtacctcg 360gccgcgacca cgctaatcga attcccgcgg ccgccatggc ggccgggagc atgcgacgtc 420gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcag ttttacaacg 480tcgtgactgg gaaaaccctg gcgttaccca acttaatagc cttgcagcac atcccccctt 540<210>101<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>101gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgatttcga gcggccgccc60gggcaggtac ctgcatcaag cctgtgacga ggaaggtagt tctgaagcac agtccgtcca 120atgtccatag tatttgctcc ttcacttacc acgaaacagt ctctaggtag ttgttcttga 180acatggtaga atactgtgta ataattcata ggcagagatt ttttagaagc tagttccttg 240gatgcagctt cattgctctt cattttttct ctcagagttt tccaccactt gctctctgga 300ggatactgcc atggtgtttt atcaagttcc tctaaaagct gcttagtgac agcatgtatg 360tttcctagca aagtaacagc gggctttaca ttattcccca attcttctgc acagatatca 420acctggataa acttcacatc tggctgatat cttggaggca gtccaaaatg taaaatccaa 480<210>102<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>102ccgcgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgatttcg agcggccgcc60cgggcaggta cctggatcct gctcctctgg gttgaaaccc gggcgccgcc aagatgccgg 120cttaccactc ttctctcatg gatcctgata ccaaactcat cggaaacatg gcactgttgc 180ctatcagaag tcaattcaaa ggacctgccc ccagagagac aaaagataca gatattgtgg 240atgaagccat ctattacttc aaggccaatg tcttcttcaa aaactatgaa attaacaatg 300aagctgatag gaccttgata tatataactc tctacatttc tgactgtctg aagaaactgc 360aaaagtgcaa ttgcaaaagc caaggtgaga aagaaatgta tacgctggga atgactaatt 420ttcccattcc tggagagcct ggtttccact taacgcaatt tatgccaaac ctgcgaacaa 480<210>103<211>260<212>DNA<213>人属 人种(Homo sapiens)<400>103gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattagcg tggtcgcggc60cgaggtactt tttttttttt tttttttttt tgacaaaacg gatgtttatt tgatgatcac 120ttaagtccaa atggcgttat ttaggctgat aattgtgata attactcctt catcttaaaa 180aggtaggcat ccatataaaa cataaaaaat gaatggaaga tatgagattt tatgggccac 240acctggaaat ggctcaaata 260<210>104<211>480<212>DNA<213>人属 人种(Homo sapiens)<400>104cgtcgcgtcg cagctcccgg ccgccatggc ggccgcggga attcgatttc gagcggccgc60ccgggcaggt acgcggggcc tcagcaggtg tgaagcgtgt gctttagttt cgtgggaggc 120ctggcatccc cgagagggag gggaaaggta accactcctt tgtggaggtc gccagggtca 180ttgtcgtgga tttgcacagt cggctgggcg gtgcaatggc ggaaagaaaa ggaacagcca 240aagtggactt tttgaagaag attgagaaag aaatccaaca gaaatgggat actgagagag 300tgtttgaggt caatgcatct aatttagaga aacagaccag caagggcaag tattttgtaa 360ccttcccata tccatatatg aatggacgcc ttcatttggg acacacgttt tctttatcca 420aatgtgagct tgctgtaggg tacgcgggat ggcacatgca gcgcaagtag gtctacaaga 480<210>105<211>540<212>DNA<213>人属 人种(Homo sapiens)<400>105acctaagctt gcatccaacg cgttgggagc tctcccatat ggtcgacctg caggcggccg60cgaattcact agtgattagc gtggtcgcgg ccgaggtaca tcttgctgga aaatgctgcc 120cagggctctg gagacggtgg ctgcccgggc tcccttcact gtccaggtcc tgaaagactc 180ttgttcatga actgtctctt cacaaagcaa gtccaccact tgctgggttt atcattctga 240gggtcgaaaa ctttctcaca aagtctcagt ccagtctctt gccttagctg ttgtaaatag 300gctctcatca cttcatcttc ctgtttgttt gcaggtttgg cataaattgc gttaagtgga 360aaaccaggct ctccaggaat gggaaaatta gtgattccca gcgtatacat ttctttctca 420ccttggcttt tggaattgca cttttgcagt ttcttcagac attcagaaat gtagagagtt 480atatatatca aggtcctatc agcttcattc ttaatttcat agtttttgaa gaagacattg 540
权利要求
1.人支气管上皮细胞恶性转化前的差减cDNA文库，由序列1至序列42组成。
2.人支气管上皮细胞恶性转化早期的差减cDNA文库，由序列43至序列77组成。
3.人支气管上皮细胞恶性转化时期的差减cDNA文库，由序列78至序列105组成。
4.人支气管上皮细胞恶性转化前与肺癌病变相关的基因克隆序列1、10、11和19。
5.人支气管上皮细胞恶性转化早期与肺癌病变相关的基因克隆序列45和66。
全文摘要
本发明的名称为人支气管上皮细胞恶性转化差异表达基因的cDNA文库及与肺癌病变相关的克隆，涉及基因的cDNA文库及与病变相关的克隆。本发明的目的是利用辐射诱发的人支气管上皮细胞体外恶性转化实验模型，构建细胞恶性转化不同时期差异表达基因的cDNA文库，以及在此文库基础上筛选出可用于阐述人肺癌发生机理和临床肺癌诊断的cDNA克隆。人支气管上皮细胞恶性转化前的差减cDNA文库，由序列1至序列42组成；恶性转化早期的差减cDNA文库，由序列43至序列77组成；恶性转化时期的差减cDNA文库，由序列78至序列105组成。与病变相关的克隆是序列1、10、11、19、45和66。
文档编号C12P19/00GK1412309SQ0210376
公开日2003年4月23日 申请日期2002年3月20日 优先权日2001年10月8日
发明者吴德昌, 范保星, 李刚, 张开泰, 楼铁柱, 葛世丽, 周平坤, 谢玲, 孙敬芬, 胡迎春, 项小琼 申请人:中国人民解放军军事医学科学院放射医学研究所