1.一种高通量基因表达谱检测试剂盒，其特征在于：该试剂盒包括逆转录反应模块、核酸纯化模块、双链cdna合成模块、测序文库构建模块；
该逆转录反应模块，包括细胞裂解液、逆转录mix、barcode板、备用96孔板；其中：
细胞裂解液：用pbs配置的0.3％triton-100；
逆转录mix：0.5μldntp,0.25μl逆转录酶,1μl5×逆转录酶buffer[250mmtris-hcl(ph8.3),375mmkcl,15mmmgcl2],0.125μldtt,0.125μlrna酶抑制剂,1μlbetaine(5m),0.045μlmgcl2；
barcode板：含96种不同标签的逆转录引物(浓度为20μm)：(5’—3’)
acactctttccctacacgacgctcttccgatctctcacatttttttttttttttttttttttttttttt(seqidno:1)
acactctttccctacacgacgctcttccgatcttactgctttttttttttttttttttttttttttttt(seqidno:2)
acactctttccctacacgacgctcttccgatctatcagctttttttttttttttttttttttttttttt(seqidno:3)
acactctttccctacacgacgctcttccgatctcgactatttttttttttttttttttttttttttttt(seqidno:4)
acactctttccctacacgacgctcttccgatctcgtcattttttttttttttttttttttttttttttt(seqidno:5)
acactctttccctacacgacgctcttccgatctctcgattttttttttttttttttttttttttttttt(seqidno:6)
acactctttccctacacgacgctcttccgatcttagcactttttttttttttttttttttttttttttt(seqidno:7)
acactctttccctacacgacgctcttccgatcttctagctttttttttttttttttttttttttttttt(seqidno:8)
acactctttccctacacgacgctcttccgatctcacgtatttttttttttttttttttttttttttttt(seqidno:9)
acactctttccctacacgacgctcttccgatctatgctctttttttttttttttttttttttttttttt(seqidno:10)
acactctttccctacacgacgctcttccgatctgctacatttttttttttttttttttttttttttttt(seqidno:11)
acactctttccctacacgacgctcttccgatctgcatcttttttttttttttttttttttttttttttt(seqidno:12)
acactctttccctacacgacgctcttccgatctacatgctttttttttttttttttttttttttttttt(seqidno:13)
acactctttccctacacgacgctcttccgatctacgtcatttttttttttttttttttttttttttttt(seqidno:14)
acactctttccctacacgacgctcttccgatctcatgcttttttttttttttttttttttttttttttt(seqidno:15)
acactctttccctacacgacgctcttccgatctctagtctttttttttttttttttttttttttttttt(seqidno:16)
acactctttccctacacgacgctcttccgatcttgcatctttttttttttttttttttttttttttttt(seqidno:17)
acactctttccctacacgacgctcttccgatctcatctgtttttttttttttttttttttttttttttt(seqidno:18)
acactctttccctacacgacgctcttccgatctcagatctttttttttttttttttttttttttttttt(seqidno:19)
acactctttccctacacgacgctcttccgatctagcacttttttttttttttttttttttttttttttt(seqidno:20)
acactctttccctacacgacgctcttccgatctactcgatttttttttttttttttttttttttttttt(seqidno:21)
acactctttccctacacgacgctcttccgatcttcacgttttttttttttttttttttttttttttttt(seqidno:22)
acactctttccctacacgacgctcttccgatctgtctactttttttttttttttttttttttttttttt(seqidno:23)
acactctttccctacacgacgctcttccgatcttctcagtttttttttttttttttttttttttttttt(seqidno:24)
acactctttccctacacgacgctcttccgatcttcagactttttttttttttttttttttttttttttt(seqidno:25)
acactctttccctacacgacgctcttccgatctgactcatttttttttttttttttttttttttttttt(seqidno:26)
acactctttccctacacgacgctcttccgatctcacagttttttttttttttttttttttttttttttt(seqidno:27)
acactctttccctacacgacgctcttccgatctacactgtttttttttttttttttttttttttttttt(seqidno:28)
acactctttccctacacgacgctcttccgatctgcagtatttttttttttttttttttttttttttttt(seqidno:29)
acactctttccctacacgacgctcttccgatctgctgattttttttttttttttttttttttttttttt(seqidno:30)
acactctttccctacacgacgctcttccgatcttagtcgtttttttttttttttttttttttttttttt(seqidno:31)
acactctttccctacacgacgctcttccgatctatgacgtttttttttttttttttttttttttttttt(seqidno:32)
acactctttccctacacgacgctcttccgatctcgatgttttttttttttttttttttttttttttttt(seqidno:33)
acactctttccctacacgacgctcttccgatctagatcgtttttttttttttttttttttttttttttt(seqidno:34)
acactctttccctacacgacgctcttccgatcttacgagtttttttttttttttttttttttttttttt(seqidno:35)
acactctttccctacacgacgctcttccgatctcgtagatttttttttttttttttttttttttttttt(seqidno:36)
acactctttccctacacgacgctcttccgatcttgtacgtttttttttttttttttttttttttttttt(seqidno:37)
acactctttccctacacgacgctcttccgatctatcgtgtttttttttttttttttttttttttttttt(seqidno:38)
acactctttccctacacgacgctcttccgatctgtgcattttttttttttttttttttttttttttttt(seqidno:39)
acactctttccctacacgacgctcttccgatctgagctatttttttttttttttttttttttttttttt(seqidno:40)
acactctttccctacacgacgctcttccgatctctcacaatttttttttttttttttttttttttttttt(seqidno:41)
acactctttccctacacgacgctcttccgatcttactgcatttttttttttttttttttttttttttttt(seqidno:42)
acactctttccctacacgacgctcttccgatctatcagcatttttttttttttttttttttttttttttt(seqidno:43)
acactctttccctacacgacgctcttccgatctcgactaatttttttttttttttttttttttttttttt(seqidno:44)
acactctttccctacacgacgctcttccgatctcgtcaattttttttttttttttttttttttttttttt(seqidno:45)
acactctttccctacacgacgctcttccgatctctcgaattttttttttttttttttttttttttttttt(seqidno:46)
acactctttccctacacgacgctcttccgatcttagcacatttttttttttttttttttttttttttttt(seqidno:47)
acactctttccctacacgacgctcttccgatcttctagcatttttttttttttttttttttttttttttt(seqidno:48)
acactctttccctacacgacgctcttccgatctcacgtaatttttttttttttttttttttttttttttt(seqidno:49)
acactctttccctacacgacgctcttccgatctatgctcatttttttttttttttttttttttttttttt(seqidno:50)
acactctttccctacacgacgctcttccgatctgctacaatttttttttttttttttttttttttttttt(seqidno:51)
acactctttccctacacgacgctcttccgatctgcatctatttttttttttttttttttttttttttttt(seqidno:52)
acactctttccctacacgacgctcttccgatctacatgcatttttttttttttttttttttttttttttt(seqidno:53)
acactctttccctacacgacgctcttccgatctacgtcaatttttttttttttttttttttttttttttt(seqidno:54)
acactctttccctacacgacgctcttccgatctcatgctatttttttttttttttttttttttttttttt(seqidno:55)
acactctttccctacacgacgctcttccgatctctagtcatttttttttttttttttttttttttttttt(seqidno:56)
acactctttccctacacgacgctcttccgatcttgcatcatttttttttttttttttttttttttttttt(seqidno:57)
acactctttccctacacgacgctcttccgatctcatctgatttttttttttttttttttttttttttttt(seqidno:58)
acactctttccctacacgacgctcttccgatctcagatcatttttttttttttttttttttttttttttt(seqidno:59)
acactctttccctacacgacgctcttccgatctagcactatttttttttttttttttttttttttttttt(seqidno:60)
acactctttccctacacgacgctcttccgatctactcgaatttttttttttttttttttttttttttttt(seqidno:61)
acactctttccctacacgacgctcttccgatcttcacgtatttttttttttttttttttttttttttttt(seqidno:62)
acactctttccctacacgacgctcttccgatctgtctacatttttttttttttttttttttttttttttt(seqidno:63)
acactctttccctacacgacgctcttccgatcttctcagatttttttttttttttttttttttttttttt(seqidno:64)
acactctttccctacacgacgctcttccgatcttcagacatttttttttttttttttttttttttttttt(seqidno:65)
acactctttccctacacgacgctcttccgatctgactcaatttttttttttttttttttttttttttttt(seqidno:66)
acactctttccctacacgacgctcttccgatctcacagtatttttttttttttttttttttttttttttt(seqidno:67)
acactctttccctacacgacgctcttccgatctacactgatttttttttttttttttttttttttttttt(seqidno:68)
acactctttccctacacgacgctcttccgatctgcagtaatttttttttttttttttttttttttttttt(seqidno:69)
acactctttccctacacgacgctcttccgatctgctgatatttttttttttttttttttttttttttttt(seqidno:70)
acactctttccctacacgacgctcttccgatcttagtcgatttttttttttttttttttttttttttttt(seqidno:71)
acactctttccctacacgacgctcttccgatctatgacgatttttttttttttttttttttttttttttt(seqidno:72)
acactctttccctacacgacgctcttccgatctcgatgtatttttttttttttttttttttttttttttt(seqidno:73)
acactctttccctacacgacgctcttccgatctagatcgatttttttttttttttttttttttttttttt(seqidno:74)
acactctttccctacacgacgctcttccgatcttacgagatttttttttttttttttttttttttttttt(seqidno:75)
acactctttccctacacgacgctcttccgatctcgtagaatttttttttttttttttttttttttttttt(seqidno:76)
acactctttccctacacgacgctcttccgatcttgtacgatttttttttttttttttttttttttttttt(seqidno:77)
acactctttccctacacgacgctcttccgatctatcgtgatttttttttttttttttttttttttttttt(seqidno:78)
acactctttccctacacgacgctcttccgatctgtgcatatttttttttttttttttttttttttttttt(seqidno:79)
acactctttccctacacgacgctcttccgatctgagctaatttttttttttttttttttttttttttttt(seqidno:80)
acactctttccctacacgacgctcttccgatcttcagacgtttttttttttttttttttttttttttttt(seqidno:81)
acactctttccctacacgacgctcttccgatctgactcagtttttttttttttttttttttttttttttt(seqidno:82)
acactctttccctacacgacgctcttccgatctcacagtgtttttttttttttttttttttttttttttt(seqidno:83)
acactctttccctacacgacgctcttccgatctacactggtttttttttttttttttttttttttttttt(seqidno:84)
acactctttccctacacgacgctcttccgatctgcagtagtttttttttttttttttttttttttttttt(seqidno:85)
acactctttccctacacgacgctcttccgatctgctgatgtttttttttttttttttttttttttttttt(seqidno:86)
acactctttccctacacgacgctcttccgatcttagtcggtttttttttttttttttttttttttttttt(seqidno:87)
acactctttccctacacgacgctcttccgatctatgacggtttttttttttttttttttttttttttttt(seqidno:88)
acactctttccctacacgacgctcttccgatctcgatgtgtttttttttttttttttttttttttttttt(seqidno:89)
acactctttccctacacgacgctcttccgatctagatcggtttttttttttttttttttttttttttttt(seqidno:90)
acactctttccctacacgacgctcttccgatcttacgaggtttttttttttttttttttttttttttttt(seqidno:91)
acactctttccctacacgacgctcttccgatctcgtagagtttttttttttttttttttttttttttttt(seqidno:92)
acactctttccctacacgacgctcttccgatcttgtacggtttttttttttttttttttttttttttttt(seqidno:93)
acactctttccctacacgacgctcttccgatctatcgtggtttttttttttttttttttttttttttttt(seqidno:94)
acactctttccctacacgacgctcttccgatctgtgcatgtttttttttttttttttttttttttttttt(seqidno:95)
acactctttccctacacgacgctcttccgatctgagctagtttttttttttttttttttttttttttttt(seqidno:96)
该核酸纯化模块包括纯化用超顺磁性磁珠；
该双链cdna合成模块包括：双链合成buffer(10x)、双链合成enzymemix；
双链合成buffer(10x):200mmtris-hcl,120mm(nh4)2so4,50mmmgcl2,1.6mmb-nad,1.9mmdntpseach,ph7.4；
双链合成enzymemix:6,000units/mldnapolymerasei(e.coli)5,000units/mlrnaseh,25,000units/mle.colidnaligase,10mmtris-hcl(ph7.5),50mmkcl,1mmdtt,0.1mmedta，50％glycerol；
该测序文库构建模块包括片段化酶mix、中和buffer、引物1，引物2、pcrmix；其中：
片段化酶mix：nexteraxt(illumina)试剂盒中的tagmentdnabuffer和amplificationtagmentmix的混合物；
中和buffer：nexteraxt(illumina)试剂盒中的ntbuffer；
引物1:
序列为5’-aatgatacggcgaccaccgagatctacactctttccctacacg-3’(seqidno:97)，浓度为5μm；
引物2:
含12种不同标签的引物，浓度为5μm：
5’-caagcagaagacggcatacgagattcgccttagtctcgtgggctcgg-3’(seqidno:98)
5’-caagcagaagacggcatacgagatctagtacggtctcgtgggctcgg-3’(seqidno:99)
5’-caagcagaagacggcatacgagatttctgcctgtctcgtgggctcgg-3’(seqidno:100)
5’-caagcagaagacggcatacgagatgctcaggagtctcgtgggctcgg-3’(seqidno:101)
5’-caagcagaagacggcatacgagataggagtccgtctcgtgggctcgg-3’(seqidno:102)
5’-caagcagaagacggcatacgagatcatgcctagtctcgtgggctcgg-3’(seqidno:103)
5’-caagcagaagacggcatacgagatgtagagaggtctcgtgggctcgg-3’(seqidno:104)
5’-caagcagaagacggcatacgagatcctctctggtctcgtgggctcgg-3’(seqidno:105)
5’-caagcagaagacggcatacgagatagcgtagcgtctcgtgggctcgg-3’(seqidno:106)
5’-caagcagaagacggcatacgagatcagcctcggtctcgtgggctcgg-3’(seqidno:107)
5’-caagcagaagacggcatacgagattgcctcttgtctcgtgggctcgg-3’(seqidno:108)
5’-caagcagaagacggcatacgagattcctctacgtctcgtgggctcgg-3’(seqidno:109)
pcrmix：166.5mmkcl，33.3mmtris-hcl(ph8.3)，6.66mmmgcl2，1mmdntp,0.5u高保真dnapolymerase。
2.权利要求1所述的高通量基因表达谱检测试剂盒在高通量基因表达谱检测中的应用。