一类新的在人类肝癌中特异表达的表达序列标签的制作方法

专利名称：一类新的在人类肝癌中特异表达的表达序列标签的制作方法
技术领域：
本发明涉及生物技术领域，具体地，本发明涉及一类新的在人类肝癌中特异表达的表达序列标签。
背景技术：
肝脏的恶性肿瘤80％来自于肝细胞，所以肝脏肿瘤多称为肝细胞癌或肝细胞(hepatocellular cancer-HCC or hepatoma)，肝癌是目前世界上比较多见的恶性肿瘤之一。在亚洲它是最常见的肿瘤占所有癌中的20-30％。中国肝癌的发生率又高达45％，而且近年的研究表明，发生率仍有继续上升的趋势。尽管对进展中的肿瘤进行常规手术切除可以延长部分患者的生存期，但是大多数晚期肝癌患者生存机会仍然很小。肝癌已经严重危害了人们的生命健康。
生物基因组中可转录表达的序列(即基因)仅占总序列的3-5％，对这部分序列进行测定，将直接导致新基因的发现，并获取基因组中与产业化关系最为密切的信息。20世纪80年代，高通量的自动测序的出现，使从质粒互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库随机选取许多cDNA克隆和决定来自非载体两端的几百个碱基的DNA序列成为可能。这些短的DNA序列叫做“表达序列标签”(Expressed Sequence Tags，简称ESTs)。表达序列标签的概念最早是由Adams等在1992年提出来的(Nature，355,642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，etal.Nature Genetics，2,173-179)针对获得大量信使核糖核酸(mRNA)序列的迫切需要，提出大规模互补脱氧核糖核酸(cDNA)测序的研究战略。随后Venter创立了大规模表达序列标签技术。其基本特征就是从以质粒为载体，构建完成的目的组织互补脱氧核糖核酸(Complementary DNA，简称cDNA)文库中，随机选择许多cDNA克隆，利用质粒上携带的通用引物对cDNA两端进行一轮脱氧核糖核酸序列测定，所获得的来自3’端或5’端的几百个碱基的非载体短脱氧核糖核酸(DNA)序列。简而言之，表达序列标签是来自表达基因片段3’端或5’端的短脱氧核糖核酸序列，代表一个表达基因的部分转录片段。
表达序列标签可用于新基因克隆、人类基因组图谱绘制、基因组序列编码区的确定等。如果一个表达序列标签在基因组中只出现一次，那么它可以作为序列标签位点(STS)。由表达序列标签构建的物理图谱叫表达图或转录图(expression or transcript map)。利用表达序列标签进行基因图制作，可以加快序列标签位点的制作和新基因的染色体定位。表达序列标签可以作为基因特异性探针，对组织特异性基因表达的研究具有重要的作用。表达序列标签还可以进行新基因的遗传进化关系分析。表达序列标签可以对所有动植物的基因作为一种数据库，通过不同的序列比较可以获得保守序列片段，从而获得基因的遗传进化图谱。
由本发明的在人类肝癌中特异表达的表达序列标签，可以方便的寻找出在人类肝癌中的特异表达相关基因，从而在研究肝癌的致病机理以及开发治疗肝癌的药物中发挥重要作用。

发明内容
本发明的目的在于提供一类在人类肝癌中特异表达的表达序列标签。
本发明的上述目的是通过如下技术方案来实现的本发明提供了一类分离出的在人类肝癌中特异表达的表达序列标签的序列，其包括(a)SEQ ID No.1～SEQ ID No.34所示的序列；(b)SEQ ID No.1～SEQ ID No.34所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.34所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
本发明还提供了一种探针分子，所述的探针分子含有上述序列中约8-100个连续的核苷酸。
由本发明的在人类肝癌中特异表达的表达序列标签，可以方便的寻找出在人类肝癌中特异表达的相关基因，从而在研究肝癌的致病机理以及开发治疗肝癌的药物中发挥重要作用。
具体实施例方式
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不是限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件如Sambrook等人，分子克隆实验室手册(New YorkColdSpring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1人肝癌组织的mRNA的分离组织分离(Tissue isolation)肝脏来源于5个成年男性肝癌患者，在肝癌切除手术后，将肝癌组织和癌旁组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation)取出组织，用研钵研碎，加入盛有裂解液的50ml管，充分振荡后，再移入玻璃匀浆器内，匀浆后移至50ml新管，抽提总RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligo d(T)的纤维素柱分离总RNA中的mRNA，定量。
实施例2cDNA文库的构建(Constuction of cDNA library)以mRNA为模板，合成双链cDNA。补平末端后，加含EcoRI切点的接头。磷酸化EcoRI末端后，用XhoI限制性内切酶消化1.5小时，再进行片断分离。过柱筛选长度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，无菌水溶解，连接至Uni-ZAP XR载体(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III GoldCloning Kit(Strategene，CA9203，USA)进行包装，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)细菌。涂板并测定滴度。
实施例3测序及数据库建立(Seqencing and Database Constructing)挑选文库中有外源片段插入的克隆，扩增后抽提质粒(Qiagen Germany)，用T3和T7作为3’和5’端的通用引物，采用终止物荧光标记(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377测序仪(Perkin-Elmer，USA)上进行EST大规模测序。从中可获得本发明的在人类肝癌中特异表达的表达序列标签。测序结果用FACTURA软件去除载体序列，传输到SUN Ultra 450 Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group，USA)中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL)，将无同源性或同源性低于95％的序列视为新基因建立数据库。
实施例4基因的全长克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基础上，进行cDNA全长克隆，分两阶段进行
(1)“电子克隆”(Electronic Cloning)以新基因片段序列作为探针搜寻dbEST数据库，将重叠序列)50bp，同源性在98％以上的表达序列标签(Expressed Sequence Tag，简称“EST”)序列认为同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER软件进行连接，部分EST可以延伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(Open Reading Frame，ORF)，用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸和氨基酸水平上是否与其他物种有同源性，以帮助判别所得到的基因全长完整性如何。通过电子克隆的方法，通常可获取人肝癌相关基因的全长序列。
(2)cDNA末端快速扩增(Rapid Amplification of cDNA Ends，RACE)如果通过“电子克隆”方法仍未得到完整的cDNA全长，则在已有序列5’或3’端设计引物，在人类肝脏Marathon-Ready cDNA文库(Clontech Lab，Inc，USA)中进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软件分析被延长的序列有无完整的ORF，如无，重复上述过程直至获得全长。
(3)RT-PCR对于5’和3’端的已知的序列，如果中间有一段间隙(gap)无法从已有的公共数据库或自身数据库获得，可考虑采用RT-PCR的方法。在序列5’端设计引物，3’端引物采用Oligo-dT，在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最后拼接便获得全长。
通过组合使用上述3种方法，可获得人肝癌相关蛋白的全长编码序列。
序列表<110>上海人类基因组研究中心<120>一类新的在人类肝癌中特异表达的表达序列标签<130>NP-1239<160>34<170>PatentIn version 3.2<210>1<211>1787<212>DNA<213>Homo sapiens<400>1tgagggggtc cgtttaaggc tactcaagtt cttctgactc tccagtcaga attactcaat 60catttagggt agggccatac atacacatgg gggaaatgtc agggtttggc gttaagaaag 120ctaggaaaga aataggtatg ggaaagtatt tcatgggtgc aatggttgat gatccagcat 180ctactttcct agtgctctac agctggaaaa ggcagaggca atggctttaa ttttatttgc 240aaagctagtt aagagtcgat ggaagaactg acactatttg ataaactgtg cccaaactct 300agatgaacac tatttcaaag agcattaagc acagatataa gagcagactc aaaacagacc 360acctggattt gattctcagc tctgccactt attagctggg aacatccaga cacattttga 420acatgtcttt gctttggttt cctcatttac ggaatgaggg tataatagta atttacctgt 480tgaagttatt atacataaca tacttacaac attgtctggt acaagtaaat gatagttatt 540tttattgaga aaatagcacc ctagttacat aatagtttct tacacaagtt tgtcaaattc 600tggagtaggg aggcatcttg gacagagaga tgtgtaaaac tgactagctc tggcactcaa 660ttctcacctc tctttctccc ttttttccta ctcactaatt tttagtgtgt tccccttcct 720cttttttttc tttctctcat aattctatac attcccaatt gacttttccc ataagcccgt 780tgagagagcc agagcaagtt ttattaactc caattcacta aaatgcaggc aagtgaagta 840gtttatccac ggctcacagg gtgaacttca cacatctgcc ttgagaacct gatccttcct 900cttggagcaa tttgcttctg atttcttggt ggagaatcag tagcgatggg tttaagggct 960ctgggaagac tagaaagttg ccctctctaa acacatttat tgaaagtggc accttagaaa 1020
aaaagggact atgtaaactc aatattatta agtatgatgg aaaagtataa agtatgtaga 1080aagcaggcat tagtaccgtt tgacattcaa catcagtaga ggtcagatca tgaggcacac 1140agagaaaata gtcaaaaggg gcactttttt ataatgtgat tgctactcca ccttgcccac 1200cttctggtat tagaaacagc ctttctgtga ttatgcctat gggtgattga gtagtaacac 1260aatgacttca tttccatttc ccagctttcc tgtgggaagg tttggatcca gcctgcaaga 1320gccatgattg taggagtata atgataactg atgtttatac agtactttac aattttcaca 1380aggatttcca tctattggct tctcactaca cccattatta ccattcccac ttcacaggca 1440agtaaactga gtccacaggt aagcaaggct aacagagttt gcatagataa tctcacagag 1500ctaacaccag aactcaggtc tgtagtgctt cttctacctt ccacttatgt ggaatagttt 1560gatgtttaat attttaagca ggtaagtatt tgagtcctct ctacccctta ccagcactgt 1620tacctggaat agctcctgaa tcttcagctt cctcatgaaa cagaggaaat aatactattt 1680atgagatttg aatggactaa attatgtaaa gcactgagaa cattgcttag cccatagtaa 1740gtactcaata aaattctagc tgtcattaaa aaaaaaaaaa aaaaaaa 1787<210>2<211>939<212>DNA<213>Homo sapiens<400>2gggcagcgaa tcttgtgatc agttgctgcc tcttcttgcc gttgtttcct ttacccctct 60gcttaccgaa aataattgct gttcttttaa tgccaaggaa gttcccattt ataaactgaa 120gttagttaga taattaggta gcttagaaca gtggctttta aactttttga ttggctctac 180tataaaaaat tcaatttgta ttgaaaccca tagacatgtg aatgtttatg ctgaaacaga 240agtatttact ttctgataat aagtgtatgt agtcagatac agtcttttaa gtccactttg 300tatggtacag aacaaatccc taacaagcta attccaaccc actaaattga tttaatgatc 360actgagggtt gtcttaccat ggtttcaaaa catttagaag gcactactca tgagttgccc 420catttcagca tatgatcccc acttttgctg gtctaagaat catttatcag tcccactcag 480ttatcatatc aaccagattt gttagtgact ttaaaaaaac gttaatttgt tacttcagca 540
agtagaagat ggatattttt gatgcccaga gtgttgtctt ccccgtccag ggtatctcat 600tctgatcaaa ccgaattggc catccatact gagaagtggc tattgatata tttatattga 660gaaatgaata tgtgtttata ctttctcccc cagatattag agaaactgaa gtgggaaaca 720cctttgtagc taccagatta tgtattctct tttggaaaag cctcatggaa aacactgctg 780tgctgtcttc cagcttccag gtttcacgaa ggcacatctc tcaactagta atatgttctg 840ttttcagaac acacttcagt acatttgatt agttcagcat ttccccgctg tttgttgggg 900tactaataga tttgtctgcc caaaaaaaaa aaaaaaaaa 939<210>3<211>1469<212>DNA<213>Homo sapiens<400>3gcacgaggtg ggtctacctt tatttaaatg atttttctgt gttctgaata ttgtggagtc 60ttctatttgg aattattgtt agaattctga agtcccactt tgggggaaaa aattattcat 120tccagagcca tatacaacct gtttctcttt ctgtgatttc ttcccttcct acttcacttt 180catgagttca tcctatgtca tctttctgtc cctcccaatg tcaaaggccc tgaggaaagg 240gcctggtgaa atggactctg gagttagact gaggcccaac agcctaacaa gccttaagta 300aatgtgtgat ctttctgcct gtttcatcca ggtatcacta acttagtcca tgttccagcg 360atgacctgtt acttctacat aacagaaaaa taaccccaac ttaagtacaa acaaaataaa 420atgaaaagga atttattggg gaatcagaga aacagctata aagttttaag cccttcctag 480agcctcaggg ctagaactgg ccctcagaat atttttcctt gtctttattt ctctttgact 540gttggctttt cctctcctac tacaggcaga ttcttccatt gagtcaggaa taatgaccaa 600caggatccct atgctcatat caccttagca gtcattaagg aaagaaggat taggactgag 660tttctttctt tcattacctt tgattactct ggttccagag ccaaaaaagc ctttgttatc 720tttaaataca agtgaaatac tactgaattt tactacatgg cagatactgt tttatgaggt 780ttccatgatt aagtaattta atcctcacag gaactattac agataatcat ataaagtagg 840tataattcca tattataccc attttacaga tgaagatgtt gaagaactaa ataatgaaat 900
aacttttcca tcacacagct agtaaatggc agagccagga ttcagtataa ggaaatctac 960gccagggaaa atgcacttgt gaacttgcca tggtttggag gtttcctaag tttttacata 1020ggcccctccc aggaaatggc aattcatgtt ctaaaacagt tttttaaaac taaaacctaa 1080tcctctttga aaagatagta aatttgaaat acatttatag gtacattttt gatagtgtgc 1140aattcaggac tcagagttgg aaaacaatta acctcaggtt tatttgggta gctggaaaaa 1200ttaaaggact aaatgtaatt ttggctggag agtaaatgat catgtaaagc aaagacctgt 1260ccaaaaaatt tcatattgga ttcattccac cttcattatg attagccatg tttgaggttt 1320gcattatttc ttgttcttta tgtggacatt agttatctac tttataaaga atattttact 1380gtaattttta aaagaaatac cacaaatact ttgtactatt ggatgtaaat aaaacactta 1440tatgcagtga aaaaaaaaaa aaaaaaaaa 1469<210>4<211>584<212>DNA<213>Homo sapiens<400>4aaaaaagtaa aggaaaaaga aatacatggg gattataaaa ataaatgaaa tgaaaaataa 60tagcttactc ttgtgagcgt attacaaata tgatcccatt tactccttgc tttatgaagt 120aggtaccatt atttttccct tttacagagg atgaaagtga gatactagaa gtttccaaac 180ctagcgagtc tcagtaagaa gtttcacttc acaaacagat aggagaaatg atagtggatc 240agaagcatga tttaaaagaa ataataaaag gccgggcgtg gtggctcatg cctgtaatcc 300cagcactttg ggaggccgag ggaggcagat cacttgaggt caggagttca agaccagcct 360ggccaacacg gtgaaaccgt ctctactaaa aatacaaaaa ttagctgggc atggtggtgc 420gcgcctgcaa tcccagctag tccggaggct gaggcaggag aatcgcttga tcctgggagg 480cggaagttgc agtgagccga gatcgtgcca ctgtactcca gcctgcgaca gggcaagact 540ccgtctcaaa taaataaata aataaataaa aaaaaaaaaa aaaa584<210>5<211>1696
<212>DNA<213>Homo sapiens<400>5ctggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa 60ttcggcacga gggaattaac tctataagga agtattctga taatggcttt caattatagg 120acatatctaa taacatgatt acatactctc ttaatagtgt agcactatag ttgttattta 180tttcccacac tgaacaaata taattatcat atacaccctt tccattatta gctctctcca 240tttttaatga aaacagtggt tggtggttta taagaagttt tttgtcggtg tgatctttca 300catcagtttg ctacccagaa tttaaacatt ccttttttgc tgctgtttta atttgaaata 360agaatggtag cccccaggtc ccctaacagt gggtgctgat ttcacagtgg gcttacaaat 420gctcttcaag agtatctcag tgttgagtcc agccacccaa gggagactgg ttcttcactg 480tgcatcatgg ccttttaatg ccattttgct tttttatcat ccaatcagca gatgttcttt 540cttcattcac gttgaaagca cttcgctatg gaaacgagta aaggtcattg gcttattttt 600tgtttgtttt gttttctgat gtcctgacag cagaatcaat cttcggaatc cttttcaata 660aaaaaaaaaa ttaccaaaac aaatatgatc acagaagctt ttaaaatctg aaatcccagg 720aagctaatta gtgcatcctt ttaagatggc ttcctgcatt tcgatataag cataagcttg 780ggtttcagat gcagttggca gtttgccatg agtcagacag gtcatcctga ctctcaggaa 840cacggagctc ctgcagaagc ctgtggagcg agggcaagtt gggatcctcc cctgtgttag 900cacgtgactg tcctctgtca ccatgtagca ggtggaggcc aggaactaca ttttcttggg 960ttcctggtgg ttagttgtac gccccatgaa cttctctcat ttccttaatt atatccaaaa 1020taatgccctt gttttatttc cccacttggc actgagcctt agggagcagg aactgtgtat 1080atcccacagc tttgcatggc acttggcacg taggaatgta ttaaatgcag ctgctttcgt 1140gcaggaagcg cggacttcag atttggtcaa agatccatga tttcagagca ggcagccctt 1200ttaaacctgt atctgagtca cttcaatgcc ttgtgtttct gctctgggct gcaaactcaa 1260gagcagcaac cacttatctt ggtacctgta gtcccgagcc ctgtgtctgg ctcagagtag 1320gccctacata aatgtttgct cctcctcact gagtggctgc atgaattgca tccagcatgt 1380ctactctggg aaagaacctg tggagcctct ttaggagtgt gtttttgccc tccctcggaa 1440
tctggtgatc tcactaccaa tggcaaagac aaactgtgag gtgttttttt gtttgtgtgc 1500acgcgcacgt atgtgtgtgt gtaaaaaggc catggaaata taaaattgta acctaaatgc 1560cttcacggcc agttatggtg gctcacgcct gtaattccag cactctggga ggccaagatg 1620agaggatcac ttgagcctag aagtttgaga ccagtcaggg caatatagac tacctgcaaa 1680aaaaaaaaaa aaaaaa 1696<210>6<211>808<212>DNA<213>Homo sapiens<400>6aatcagatgc ctagagggac ctgaatgcta caacctcgga gagagaatag gggagtgttg 60gtgtttgtgg tgaatcggaa agcacgtgtt taaagaggac aatcacatta attcagttcc 120agctatgaga ttacattgtc aaatcatctg atttttcaag agaaatcaca aatttgaatt 180tcttaaaatg agaaatctac tttttaaata atgacgggat tttcaagtat gttaaaaata 240ctgtaggcta ataccgaata agctaagcaa aacttttgtg gatcaaaccg aaaccatggg 300ctattttctt ctcctcctct tcctcctcct tctcatagga ataaaacaac taaatataat 360cctgtcttta taatttccat ataccaagct ttaattatat ttaataccca tcaaagttag 420ttaaaatgaa ctctgagtaa tttatacctt atggaaaaat tacggtgttc taagtctgac 480caaatgtgta gaaagtacca tattatcttt ggatatgcca tctatgtctg ctttgcccca 540ctatactaca tattgcttta gatcagccac tattttacat ttatttcaaa agaaattttg 600catacccttc aacacctagc actgttatat atgtggtcag tgcttaatga cttggagtaa 660attagtggta aagtaaggga atttaaaagt agcattttct tctttccacc tccacaaaca 720aaatcagaat ctaacttcat ggaacatttc atctaatttg ttttaaataa agacaaactt 780ctaaaaatta aaaaaaaaaa aaaaaaaa 808<210>7<211>841<212>DNA<213>Homo sapiens
<400>7gcaagactcc gtctcaaaaa aagaaaaaaa agaaaaagaa atgaacagga cttggaatac 60taggaaaact ctgtaccaac aaattaggtg aaactgacaa actcctagag ggacacaaat 120cacttaaatt ggctcagaaa tctgagaaat ttaatagaaa atctaaatag acctataaca 180agaaattata gtaatgaaaa gtcctgagtc ttcctacaga aagcctgtgc ccacgtggct 240gccactgtat actgtcaata acaccaacca ttcacaatct tcagaatgtg gaggatgaga 300cacgcccatc tgagtctaag aggctaaagg cagcgaaagg catcataatc cgtgccccca 360ccccacagaa caatgtccat catgaataca gacataaaga tccttaacaa aatattagca 420acccaaactc ggcaacatca tacaccaaga ccacgtgggt gtatcacagc aaagtgaggt 480tgcttaaaca tctacgaagc acttaacgtc ataagccaca ttaatagaaa aacagatgcc 540tgtaatccca gcactttggg aggctgaggt gggcggattg cttgagccca ggagttcaag 600actagcctgg gcaatatagc gaaaccctgt ctctaccaaa ataaacaaac aaaaaaaaca 660aaaattagcc aggcatggat gggagtgcat gcttatggtc ttagctactc acaaggctga 720aaccagagga tcatttgcgc ccaggaggtg caagtccagt gagctgagat cgtgccactg 780cactctagcc tgggaaacag ccagagcctg tttcctaaaa aaaaaaaaaa aaaaaaaaaa 840a 841<210>8<211>560<212>DNA<213>Homo sapiens<400>8gagagaacta gtctcgagtt cttatttttg atctagttat ctccctctct atcagggcac 60cctctatttc tatctgtatt aaccctctga aaacaggaat acacagaggc cactccaaac 120atggacattt gcaatgccta gaacattgtg ccagtggata tgtgaatgaa ttattccctg 180tcccccacct gcacattatt ccgttttctt gctcattgcc actgtatcag aagggccttt 240ctttactcct aaccatatta gcaccatctt gtcacatgtc tttccctacc tcttatgatc 300tagatcatta attctggagt tttaatttta aggtatacta tatgggaaaa ggaggcacct 360
caggagttca tgagaaatag catgaaaagc ttgggatgct tattccatgg aacttaagac 420atttgctgag gttccagagt attatcaaaa aatttaagaa gaaaaatcag atttttaata 480caaagagcac atcatagatg tggtttgtag tttgtagacc caaaaaaaaa aaaaaaaaaa 540aaaaaaaaaa aaaaaaaaaa 560<210>9<211>838<212>DNA<213>Homo sapiens<400>9gaggcggccg ctctagaact agtggatccc ccgggctgca ggaattcggc acgaggactt 60attgaattgt ccacttaaaa ttggtccatt ttcttccata taaattacac ctcagaaaag 120ttaatttctt aaagcaaaaa atacgaaatt aaacaaagaa aaagaaacta ttgtaattaa 180tcctggatta cacactttac taaagtacag tgctcaaatt gataagatgc agccaaattt 240ctactcactt tgtgctgtgt agaccctgct agtttcctgt gcctattcta gaagctgtgg 300tagaaatatg gccattagag aggaaaggcc cgggtttccc ttctggaacc atcatctacc 360accagatggg catatggcat gttagttgcc tccaaatctc ccctgcttca agagtcctcc 420acctccacct ccaacaaaga gtaccttctc tgagcatctc tggagcttct tcttcaatct 480gattctatct gtttttcctt gctaggattc ctcacagttt atggtccatg ggaagattcc 540tagtctagtc tctgaataca tacatgtgta tatatatgta tgaacatgtg tgtgcatata 600tgtatatgta tgtattgcac acgtatgtgt gtgtttctat catttctaat gacctggaac 660aggggaacac tgtgtgtgct tagtccacct tcttgtaact ctcagagctt agtttcaaat 720aatgacataa aggagctgca caaatggaaa gcaaataaaa ggcctataat taaagccagt 780gtattatttc gactgaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 838<210>10<211>838<212>DNA<213>Homo sapiens<400>10gaggcggccg ctctagaact agtggatccc ccgggctgca ggaattcggc acgaggactt 60
attgaattgt ccacttaaaa ttggtccatt ttcttccata taaattacac ctcagaaaag 120ttaatttctt aaagcaaaaa atacgaaatt aaacaaagaa aaagaaacta ttgtaattaa 180tcctggatta cacactttac taaagtacag tgctcaaatt gataagatgc agccaaattt 240ctactcactt tgtgctgtgt agaccctgct agtttcctgt gcctattcta gaagctgtgg 300tagaaatatg gccattagag aggaaaggcc cgggtttccc ttctggaacc atcatctacc 360accagatggg catatggcat gttagttgcc tccaaatctc ccctgcttca agagtcctcc 420acctccacct ccaacaaaga gtaccttctc tgagcatctc tggagcttct tcttcaatct 480gattctatct gtttttcctt gctaggattc ctcacagttt atggtccatg ggaagattcc 540tagtctagtc tctgaataca tacatgtgta tatatatgta tgaacatgtg tgtgcatata 600tgtatatgta tgtattgcac acgtatgtgt gtgtttctat catttctaat gacctggaac 660aggggaacac tgtgtgtgct tagtccacct tcttgtaact ctcagagctt agtttcaaat 720aatgacataa aggagctgca caaatggaaa gcaaataaaa ggcctataat taaagccagt 780gtattatttc gactgaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 838<210>11<211>1049<212>DNA<213>Homo sapiens<400>11gctacaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcgg 60cacgagctcg agtttttttt tttccaataa ggagcaacat ggataagttt aagctactct 120ttcaaggtca cacttgtaag cattagattt tcattctaaa acacatatgt catctcataa 180aaatatatct gcgtaatgta ttctgtctca tgtaaattaa catagaaaag actaatgggt 240tcttccttat ctctatctaa tgttatgatc ctattggggg gactggacag gctttctaat 300acccagattt actgtgttta aggtgttcgt ataaggccaa tagacactag gaccaagtaa 360tgcatctcct gcaatttcct cttttcacag caggaaaatc ttaacgagtt ccaaattctg 420ggcttagagg agcttctgat ggtacagtat gccaacatct acttgttttg gatggcaaca 480ttgttctgat tcataaggaa tttgaaagaa aatacattgt agaattaata ttagttaacc 540
aaacaaaatt tattaaagga tatgttggtg aaggagttga attatttgag gaaaaaacat 600ctgctctctt gtcaggactt ttctgtctcc aggagcttct caactttgca gatactctag 660aatggtattg catgcaaaat aatgaccgcc aagatgttcc tgccctaatc cccagaacct 720atgaatatgt tatattgcaa agaaattaag gttcaaacag aattaaggtt gccaatcagc 780taaacttaaa atagggagac tctcctagat aatctgggtg ggtctatttc aatcagtgga 840aaggccataa aagcagagtt gagtcttccc gtgagatgaa aataaatttt gtttaaggac 900agcagcttca gcttatgccc cagaattcca gcctgcccct cctaactgcc tgctgtacag 960attttggact tgtccagcca cgtttcaaaa ctgagtaagt cacttccctt gcaataaatt 1020gattactaca caaaaaaaaa aaaaaaaaa 1049<210>12<211>951<212>DNA<213>Homo sapiens<400>12cttcctaaaa gctggacgtc caccgcggtg gcggccgctc tagaactagt ggatcccccg 60ggctgcagga attcggcacg agccacgcat aagccaaggt gaacaggcag ccgcaggaag 120ggcgtccaga tgggtgagtc gctgggaccc tgtgcagact cccgaggggc ggtggagccc 180acagaaaatg cagtggggct gcagagcctg gactccccac ccaggccaga tgtcctcagc 240tcaagaccca ccaggcgctt cctccccgct ggtctacagt gcctgaaacg tgggcctccc 300cgacgcgggc agtgggtctg ttggagtggc cagaagggtc ctgttaggga acctgtggat 360ggctcaggat tttagaatac tccgagcctg gggtgcccag agagcggacc ttcggaaccc 420acatgagtgt ctctgcttgt gactcccggc agctccttaa tcctaaagaa gccagatccg 480aatcttgcca aattgtagcc aggagttctc gagcagccag agagcgatgg atttccaccg 540gcagaggaaa gatccagctt taggagcaga atcacacctg ccagctggcc ctagcaagct 600gtggtctctg ggaggccagg gacgagagcc cactccctcc tccgttcatg gctcagggtc 660gtggccagag cctgcctgtg tagaggggaa tccagcagcc cccagccccg cccagctctg 720cccacacagc tgcaaggcca gcatcctgcc ctttgtcaag ttcaccacag tcatgtccca 780
ccccccattc gctgaaatca gccccaaatt gcctggaaac ccagaccccg caccccctcc 840ccagctttcc tttttcatgg agtttttatt tcagaacaag gaaacagata ccccccaaac 900aattaaagaa ataaatctat aattaccaat tgtaaaaaaa aaaaaaaaaa a951<210>13<211>2144<212>DNA<213>Homo sapiens<400>13aaaactagtg gatcccccgg gctgcaggaa ttcggcacga ggggaccagg ctgtgtgctt 60gctaactgtg cttcaggaat ccgctctggg atgccttcct gtctggcaaa aggggttacc 120cccagttcta cccagtccct gtcctcggtc acatgctgcc gccgcctctt cccccgcggt 180gccgggagcc cgcgttttgt ttgtcagcag ttaggcggtg agtatttata gacgccacgg 240cagtcctgcg gtgcgcaagg tttcaattac tgcgatgcgc cccacaagac gaaaggttca 300cgcgctgcgt ctgagctgca cgtctgcccc ggagacccgc gtggaaaaag ctctgggctg 360gaatctccgg gcttccctgc ttgggcagaa ttctggacct gttgcccaga cgacacgtgc 420acattcagat gacccatgct aaggaccaaa gggatgccac cggcccaatg tactgccttc 480atcccccacc atcctcaagg gacccttgct gatcaagccc gggagactac cctttttgac 540ctctctttgg tgatcctgac caccgtagaa atttgcagtc atgacatttc attgccctac 600atactgcaaa atgtcaaagt gctgagctgt ggtaaattgc tctggggtgg aaggcgctgt 660tactgacaac acgaagcagc tagcgggtca ggaggggaaa tggagcttgc agaaactaat 720ttagccacat ttaaaattta gcccctggta aggttcccag tatgactgac gaaggtgcag 780ggctcctgtc tgtggaacct ggctggttag tcttctctgg ttgcagccct agaatcagga 840tcaagggtgc tgtttagagc ggtttctgta gaatgtagcc tttcagataa gggaagtgat 900gcttggtcaa tccacaaagt tgtcttcagt ttttccaact gcaaccattg ttctttgagt 960atgttactga tgcttctaac aactggaacc actaatatgt aaaagaagaa aggcactaga 1020aataagaaag tctaactagc ttatttctca aaggcttagt ttagactact ttgtttctca 1080agggtgggac ctcatttctt tcaggtccag agggccatgg atatatattt atttctgtgg 1140
gtgaaatttc aataatgttc tgtcttagaa cattacaaat atgtgccact tgttttaaaa 1200aggggaaagg tgcaaatgcc tcttgaggtc ttaatgatgt cttaaacttt gcactgagag 1260actttgataa tattatatac tcctcatgca aacaatgata taggtactat tatatcccta 1320tcatatagat gagcccactg aggcttggag aatttaagta atttgtctaa agccatacac 1380acagggatgg agctgagaat ggattacaga tttgttactc cagactcata gtctggacat 1440gtctttccct tcaattctcc attgttgatt agctcacgtt atagttgatt agatggatgg 1500ctttttattt attttcaaaa tttcacagaa tgttacaaat tggctcagtt attggggaaa 1560aaagccctaa tcttgtcttt agaaaactgt tatattggtt atctgaaaat ctcttgctga 1620tagtccagac ctaaatcata atcctcactt tgctccttga cttaatgtat cttcagcatt 1680aaagccatct atctgtagaa aattgaattc agtaaggact ttgtggatta cttaattttt 1740tcagatttct ccatactaat gatttgtagg tatgcatgtg tctgttttcc tgtggctgtt 1800ccttttgtac aaggtaaaat atgctgaaat ctctcttaaa ttgcagcctt tttccttttc 1860attctttttg cagagctaga cccaatacag aattgatttg aaaaacagtg tgtgagttcc 1920tctgaaagat ccttacaatg tctaatgttc atcattaaag gtttgttctc aagagttgcc 1980tctctgtatg taaaatgtaa atacatgtta aaggcaatgc ttgtagaaat gaccctttgt 2040taacaagtca gtgtgaatta atattttagt tgctgaaata taggaataaa atattttgtg 2100gattttgtcc gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa2144<210>14<211>1017<212>DNA<213>Homo sapiens<400>14agactgggac tcccccgcgg tggcggccgc tctaactagt ggatcccccg ggctgcagga 60attcggcacg agaagctaat gaaatcgtat ataatcaaat cacagactgt ttagtgcaat 120taatgattta gtagcctgga ccgtcactca actccagcga tcttgtcctg aacctttatt 180ttaatcatga tgccaatcag gacttttcag gatgtggtca gtactatatg agctaacatg 240gcaatgagct ttgaatagta atccattttt tcagcactct tcagctacat gacaagagcc 300
ttctagaaac tttttcagtt gtgtcttgat gggagacagt acactgaagc aaagagaact 360cagggcagga catttgagca gagatttttt aaagttcatg gaaaaacaac ggtgtggcta 420taaaacttta gttataagtc accagatgtg ataaaaataa ctgaagagat aaaataatta 480tgacaacaat ctttttttca gtaactatag ttaacctaca aaactaaaaa caccaaaaac 540tttaaaagat aaatattttt atatcttaaa accacccttg gatgtccatg tctaatagtt 600gtgcacagga gtttattcta gagttcctct tcctttgttt aatcacaagg gtgtggatca 660agtccagctt tgtttggctc aacacaaagc agcactcagg taaccaagga accatccctc 720ctgatgtcat ctcttcctta acaaccactt caaaatcaca tgaaacagta ggcctgccct 780tcctttatag aatcagatgc tgaaggccac tgggctttta gccacagctc agggaaggca 840gagaaataat ttcaatccca gctgttaact taccttgaag aacttacatg gggagaacag 900tccaggcatt ctccaggtag gaaatataaa tatagcgagc cgtattgcag gccaggccaa 960tgtacagaac cctaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 1017<210>15<211>1751<212>DNA<213>Homo sapiens<400>15ggcggccgct ctagaactag tggatccccc gggctgcagg aattcggcac gagctagtct 60cgagtttttt tttttttttt ttgagacaga atcttgcttt gtcacccagg ctggagtgtt 120gaccttctgg gctcaagtga tcctcctgcc tcagcctccc gaatagctga gattacagga 180acgcaccact atgcctgcct aatttttgca ttttttgtag agattaggtc ttgccatgtt 240gcccaggctg gtctcaaacg cctgagctca agcagtcctc ccacctcagc ctcccaaagt 300cctgagatca tggtataagc cactgcacct ggcctttttc caaacagtta taatcagcca 360ctggttgaat tcacagttgg ttgaattcat ggttggttga atctgcagat gtgaaaccaa 420tggatactaa tagccaccta tattacatag tacaaaccta gctttgaaaa tagtgagaaa 480gatatgaaaa taaactagaa ttcacgggag aaaatgaggg gttctaataa aggtgtagac 540aatacattac caatacagga ggtcagagga aggggagatc tgtcaggctg gggtcttcag 600
gaatgcttca cgaagctcgt gccgaattcg gcacgagagt atatgaaggt ttctcatact 660taagttccag gtttttatct ggtaaaatgt tacacttact cggttgtaac tgaagatatg 720gtatgtttga atatttacta taagtctttc agtttgacta aaaatgtgaa agttgaattt 780agtagatgat cttcacagtt ccatatgtat aatgtgccag gtaactcacc tgccccttaa 840gaagggaacc ttgaattaca taagccgtac ctttgatgtg cctaatagtt tcagatgtct 900tagtttttta taaccatagt tgattaggcc aagaggcatt catttcttat ttaagctggc 960aaaattagca ggaattagag aagtttaaaa agataaatgg ttttatgata atgttaacca 1020tcttttgtta gtaaatatgc gttctattat tttaatcatt gatgccttac aaaagaaaac 1080atcttttcta ataccctgaa tatgtgctgt tcttagaatc atctatggat tcttttaaag 1140gttgtttgtg aaattagttt tcccttttta gaatctcagg agtagtgggg taaagacatt 1200tcctgctgtc agtggataag acagagcttg ttaactgttt tggcagtagt taaaatcaaa 1260ttgtacactt ctcagcctgg gttcatgctt catcattaat acacctcaca gtgcctaagg 1320aacatttact tactggtcag aaggtatttt ggaagagttt catattaagg aggaacaaat 1380aataatttta agttcttaaa aattacctaa aacaccccaa atataaaaag aagccttcac 1440acctattctg tctttaggat gtcttaaatt attagcagta ctcctttttt aaaaacactg 1500taaaagtaac cacaaatatg tgaggactta ctattttaaa tggaatggaa tgagctccat 1560agattagttt tgaatataaa gtatataaaa gtgcatcagt ggtttatata ggctttaaaa 1620acatgttatc ttacagtcct ttaaagcagc catagagttt gtatcatttt tcaagccaat 1680ttcagtcagg gatttgaatt gtttgattat ggatgataaa tgtgtcatat ctaaaaaaaa 1740aaaaaaaaaa a1751<210>16<211>1884<212>DNA<213>Homo sapiens<400>16aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca 60ggaattcggc acgagattca gcagaaggtc agtaaaggca tcgaccctcc tcaagtcctc 120
tcgccggaca tggtcccgcc ttcggagaga ggcacgcccg gcccggacag ttcaggctct 180ctcggctccg gggagtttac tggcgtgaag gagcttgatg acatcagtca agagattgcc 240cagttacaaa gagagaaata ttcactggaa caagacattc gagaaaagga agaggcaatc 300agacagaaaa ccagcgaggt gcaggaatta caaaatgacc tagaccggga aacaagcagt 360ttgcaggagc tcgaggctca gaaacaggat gctcaagacc gcctggacga gatggaccag 420cagaaggcca agctccgaga catgctgagc gacgtccggc agaagtgcca ggatgagact 480cagatgatct catcactgaa aacgcaaatc caatctcagg aatctgactt aaagtcccag 540gaagacgatc tgaaccgagc caagtcggag ctgaaccgat tgcagcagga ggaaacccag 600ctggagcaga gcattcaggc tgggcgagtc cagctggaaa ccatcatcaa gtccctgaag 660tcaacgcaag acgaaatcaa ccaggcaagg agcaaacttt cccagctgca tgaaagccgc 720caggaggccc acaggagcct ggagcagtat gaccaggtgc tcgatggagc ccatggtgcc 780agcctgaccg acctggccaa cctgagcgaa ggcgtctccc tggcagagag gggcagtttt 840ggagccatgg atgatccttt caaaaataaa gccttgttat ttagcaacaa cacgcaagag 900ttgcatccgg atcctttcca gacagaagac cccttcaaat ctgacccatt taaaggagct 960gaccccttca aaggcgaccc gttccagaat gacccctttg cagaacagca gacaacttca 1020acagatccat ttggagggga ccctttcaaa gaaagtgacc cattccgtgg ctctgccact 1080gacgacttct tcaagaaaca gactaagaat gagccattca cctcggatcc attcacgaaa 1140aacccttcct taccttcgaa gctcgacccc tttgaatcca gtgatccctt ttcatcctcc 1200agtgtctcct caaaaggatc agatcccttt ggaaccttag atcccttcgg aagtgggtcc 1260ttcaatagtg ctgaaggctt tgccgacttc agccagatgt ccaagccccc accttctggc 1320cctttcacct cctccttggg aggggcagga ttctcagatg acccctttaa aagtaaacag 1380gacactcctg ctctgcctcc gaagaaacct gctcctccac ggcctaaacc gcccagcggt 1440aaaagtacac ctgtaagcca gcttggttcc gcagactttc ccgaggcccc cgatccattc 1500cagccactcg gggctgacag cggcgacccg ttccaaagta aaaaggggtt tggggacccg 1560tttagtggaa aagacccatt tgtcccctcc tctgcagcta aaccttctaa ggcctctgcc 1620
tcgggctttg cagacttcac ctctgtaagt tgagtcctcc gcctccgggc caccccactc 1680ccttccgctt gcagcttccc tgggattttt gtctcctttt aaaggcaaac ctcccagctt 1740ctttagcctc ttggtacctc acactctctg tccctcgcgt tatttattct acactgccac 1800ttctgtaaga aaaacagttt ctcaataaaa aaaaaaagag ccgcaaaaaa aaaaaaaaaa 1860aaaaaaaaaa aaaaaaaaaa aaaa 1884<210>17<211>670<212>DNA<213>Homo sapiens<400>17aagctggacc tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag 60gaattcggca cgagagctgg aacatttttg aaatgctgaa agaaaagaac tatcaacttg 120gaatcccata cctggtgaaa caatccttca ggaattgggg ggaagggctg tgggggagga 180aatcatgaca ttcttacatg agggaaaaac taagggaatt tgtcactggc agatttactt 240tatagggatt actaaaggca attctctaaa caaagggagt ggtaacaaaa ggaatcttgg 300aacatcaggc aggaaaaaag gacaaaaaga gcaaacatac aggtcaatac aatagattgt 360ctttttcctc ttgagtattc taaattatgt tcaacagttg aagcaaaaat tacgcttgtc 420taagtggctg tccatatatg tagaggaaat acttaagata gttacattat aatgggagag 480taaaaggaca cagaaaaagg tttctttctt tatttgtaat ctggtagaac tttccagagg 540tatagaggta atgtttctac ctttcactca aactggtaaa atgtcaacac cagtagactg 600tgataagtta ctgcactcca gcctgggcga cagagcgaga cttgcctcaa aaaaaaaaaa 660aaaaaaaaaa 670<210>18<211>1019<212>DNA<213>Homo sapiens<400>18aattcggcac gagaaataag gctgttggag ataaacttcc tgaatgtgaa gcagtatgtg 60ggaagcccaa gaatccggca aacccagtgc agcggatcct gggtggacac ctggatgcca 120
aaggcagctt tccctggcag gctaagatgg tttcccacca taatctcacc acaggtgcca 180cgctgatcaa tgaacaatgg ctgctgacca cggctaaaaa tctcttcctg aaccattcag 240aaaatgcaac agcgaaagac attgccccta ctttaacact ctatgtgggg aaaaagcagc 300ttgtagagat tgagaaggtt gttctacacc ctaactactc ccaggtagat attgggctca 360tcaaactcca aacagaaggt gtctgttaat gagagagtga tgcccatctg cctaccttca 420aaggattatg cagaagtagg gcgtgtgggt tatgtttctg gctgggggcg aaatgccaat 480tttaaattta ctgaccatct gaagtatgtc atgctgcctg tggctgacca agaccaatgc 540ataaggcatt atgaaggcag cacagtcccc gaaaagaaga caccgaagag ccctgtaggg 600gtgcagccca tactgaatga acacaccttc tgtgctggca tgtctaagta ccaagaagac 660acctgctatg gcgatgcggg cagtgccttt gccgttcacg acctggagga ggacacctgg 720tatgcgactg ggatcttaag ctttgataag agctgtgctg tggctgagta tggtgtgtat 780gtgaaggtga cttccatcca ggactgggtt cagaagacca tagctgagaa ctaatgcaag 840gctggccgga agcccttgcc tgaaagcaag atttcagcct ggaagagggc aaagtggacg 900ggagtggaca ggagtggatg cgataagatg tggtttgaag ctgatgggtg ccagccctgc 960attgctgagt caatcaataa agagtttttt tgaccctttt taaaaaaaaa aaaaaaaaa1019<210>19<211>940<212>DNA<213>Homo sapiens<400>19gaaaggctga agaagcagaa acagggaacg aaaggtagat tggcgtttca gagttacttc 60ccttgcaggg ttaaagcatg ggggacttcc tcatcaaacc tgctcaggta aactgggccc 120cttctgatta gctgctgtgc atctcctatt tttttgaaaa gctgcccttt ttaaagttcc 180gttggacagc ctggcaccta gcaggagtga ctccattctg gtttggtctg gtccgttggg 240cctagtgtaa gagttcagtc ctcccataaa ttttatttaa gattattctt gaactactat 300tctccttgct attcatgggc tttcccaatt accaatataa cagagcctgt tctgaatcat 360ccataatcca atccttcgca tagcatatat tctgcactct gaaaatatgt ggtgagtggg 420
tgggtgagag agtgagttca ccgacccgac cgataaatcc caagcatgga gggtagaaag 480ggccctctga catcagacgg aaccaagttt acatgcaggc tcctttatgc ccagtttgtg 540tgaatttaag gaagttatct gagtctcttc aacctgtagt tccatcctct gtcaaagatg 600gatactgaaa ggctgttgca ttattttggg ggagggttac acacaatgct gtgtgtgcgt 660gtgtgtgtgt gtgtgtgtat gtgtgtgtgt gtgtgtgtgt gaatagcaag tccagaacat 720cccagtttgc atgggactgt ccaggtttta gccctgattg tatgggactg tccaggtttt 780agccctgatt gtcccgcatt ctgggaaact tcagtcgccg gtgaactgca attgttgtta 840ccttgtcccc ttccctaagc ccataaaact gttttgggtt gttactatcc attaaaaagg 900aagtgctgac ttccaaactc tcaaaaaaaa aaaaaaaaaa 940<210>20<211>1204<212>DNA<213>Homo sapiens<400>20tcccccgggc tgcaggaatt cggcacgagg aactcctggc ctcaagggtc ctcctgcctc 60ggcctcccag agtgctggga ttacaggtat gaggcactgt gcccagctgg ctctttaatc 120tttactatta gactgatttt catatgacac agtaggcata gggctctttt agttactagt 180gattccaaaa gtgactttaa tcccattact caggatctct atggtactgt caattagatc 240ttcctcattt ttgcaacatg tttacgtagc attaatgata ttattgtttc ataagtgaga 300aaatgaagac acagaaggat aaaatagttt gttgcatcag cttgcgggca taataagcaa 360cctttcttct ctctgttaat tctgttttgt aggccagtat aatggcttta gtgtcactgt 420tgttaaggag tttttgatgg gttgtttcta ttttcttttt gagatgtgaa ctgtccagat 480taattctaat ctataacttt tgggaaaagt atattgatat acatgttaga atgtgtgtgt 540gtatgtgtgt gtgttgtgtg tatgtagtaa aatgataacc tttagctttg gttttcaata 600agaattttaa aatttattac aattagaaaa tttttcctcc agtttttatt cagttgtcct 660ttttcccttt ggaaatagtg gacctgaccc aattcttttt tgagaattca tttcatagag 720cagcgtgtta gaatattttt gttcttctaa tcaagtgcag tgtaagaact gagacatcca 780
taatttcttt tctatccctt gggttaagat attaaccttt attactatat ttaactgggc 840ttggaatagc aaggcataat ctgaggagca tagctacttc atctgtgtaa taaattgctc 900tttcactaca aacattttaa tactgatttt gttggtcatt taactttcta atagtggtcc 960ttaaattatg ctctaaaaga agaataccat atatattgga aattaaattt gttgttgaat 1020aaaattagaa ttattataaa attcaacacg accttattgc tacctcagga taatttcttg 1080gcaaaaagtt gatccaaact gggtgtggtg gctcatgcat gtcatcccag cacctgggag 1140gctgagacag aaggattgct tcagcccagg agttccctga tcaaaaaaaa aaaaaaaaaa 1200aaaa1204<210>21<211>614<212>DNA<213>Homo sapiens<400>21ggccttccac cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt 60cggcacgaga ggaggaacat gaggtggctg tgctgggggc accccccagc accatccttc 120caaggtccac cgtgatcaac atccacagcg agacctccgt gcccgaccat gtcgtctggt 180ccctgttcaa caccctcttc ttgaactggt gctgtctggg cttcatagca ttcgcctact 240ccgtgaagtc tagggacagg aagatggttg gcgacgtgac cggggcccag gcctatgcct 300ccaccgccaa gtgcctgaac atctgggccc tgattctggg catcctcatg accattggat 360tcatcctgtg actggtattc ggctctgtga cagtctacca tattatgtta cagataatac 420aggaaaaacg gggttactag taccgcccat agcctgcaac ctttgcactc cactgtgcaa 480tgctggccct gcacgctggg gctgttggcc ctgccccctt ggtcctgccc tagatacagc 540agtttatacc cacacacctg tctacagtgt cattcaataa agtgcacgtg cttgtgaaaa 600aaaaaaaaaa aaaa 614<210>22<211>1363<212>DNA<213>Homo sapiens
<400>22aactggagct ccaccgcggt ggcggccgct ctagaactag tggatccccc gggctgcagg 60aattcggcac gagggagccc ccagtgccct gtggtggtgg acaccatgca gaaccagagc 120agccagctct acgcagcact gcctgagagg ctctacataa tccaggaggg caggatcctc 180tacaagggta aatctggccc ttggaactac aacccagagg aagttcgtgc tgttctggaa 240aagctccaca gttaatctgg acagatacct caattctagg tgaccaacgg gagggcttct 300caaggcttag ctctccctga gacccagctg gcttttaccc ttgacctggt gtccctacct 360gaatcactag ctcagatttt tctgatctaa gcaaacaact cccagctgag gaatgcaggc 420cacagcaccc aatcaagaca aattgttatt atcagaaaat gaagcaacac ttgagctgtt 480caggccagtt ccctgttgaa gaaacagttc cctgttgaag aaagtagagc ctgacactgc 540tcccactttg gagaccacat tccctgcaca cggtctttga gagagcagtt gcactctaca 600ggcacacttc tgaggtacgg tatctctctc cagccactct gatacaagta attcaagctg 660gcattccttc tattaggaaa ttcattttac ccaatttgca tttatggaat tgatcattta 720agacactaaa ttagttttta gaaccaatta tgggaagaat tccagttgtt aggaagagat 780gaggagttgg aagaggaggg attagaaaca ggaggaggca gtcatcctct ccttgccaaa 840agatttaaac ctgtccacat tggtggtgat gatgggtgag tttccatggt aacacatccc 900taattttacc agggaagagg agagtactca ctttaccatc tttgaatata tttcatagaa 960atctagctct ctgtaccctg aaatcttcca ctagcctcac ttttcaacag agtcatctag 1020aagggagggt tggcttccca aaagcataac cttgaccaaa ccaaacaata ggcaccagca 1080atgctgtcat tcagttatgc agaagctcat ttgtgaaatt ctgtttctct gatttcttcg 1140caagtctctt aatggtcatt tgtgttagat tacatcaaac tgatggatag ccattggtat 1200tcatctattt taactctgtg tctttacata tttgtttatg atggccacag cctaaagtac 1260acacggctgt gacttgattc aaaagaaaat gttataagat gcagtaaact aataacagaa 1320ttattaaaat atatcaggct aaaaaaaaaa aaaaaaaaaa aaa 1363<210>23<211>1990
<212>DNA<213>Homo sapiens<400>23caggggcggg aaacactgac cacagaagac aagttgagct gtatattctt tgagatactt 60gagatctttc tggttcatgc cctcatctgg aaaatggaaa taataacatc tctgcatggg 120agatgacaaa gtgtgggtac aatgcctacc acactgtagc tactcaagaa atggaagtca 180caatcattct catgccacca gcctactgag cagatcaatg ttctggtgat gttctgcatc 240cagaccatct ttcaagaact gaatggttac taccctgggt cttctaccga aagaaagaac 300atgggtgtgg gcattcaagt atagcctcta aaaaaagaac acataagtgg ttgctgggtg 360ctgttatata atcacacaca tacaaggctt aaatcatgaa tatttccttt ttgaagggga 420aaaaaagcaa aaatccaatt gtgtagagtt ttaggacaag aatattatat gaagaaaata 480tagctccaag agatagtggc tgaaagggca gaattagaga agcagaagaa gcaagaagtt 540accgcaaagg cttccaaaaa taaaatagga tgatacgccc aagaaaacaa aggctacttt 600tgctaagtca ggggtgagag aaaataatga aagatgcaaa gaagaatgac tatggagcta 660ctattttgtc tcagccttta gcacaaagaa cgaaggaagc aggagctcag taagataaat 720attaatgagt ctctgggaat atcaagaaac agaattaata agaggcaggt aaaagaatac 780ttatacaatt taaatattta taaatcagta cagctgggtg agattcattt gacaaattca 840aagaaccagc taaggcagga catacctgca atactagtgt ttgtcataga gagttatgta 900ccagggaaat ttaagcaggc tgatttaatg ttggagctta agcagcccaa agcagataca 960ggaaaaggtg ggctagttag cgcatccaaa ctaccagtca aaactctaaa tgaaatcata 1020aatcagtcca tgagcatctt cagaaaaata acatagtaag tcatagccaa agaggttcat 1080aaaaaacaga gcctgtcaaa gcaacttaat ccattttttg gaaatgctaa gaaatttgga 1140ttatagacat agtttacaga ggccctgcag atgccaccag acaactcaat ttgaacccat 1200cttggtgatg gaatggctgt tactctcact gtttccaaca gagaagggca atttcacctg 1260cacatttaat acacagggat agggttctct gttcattccc catcatagcc caggacagag 1320ttaatttagt tcttagctaa atagagtcag tgcccagaag tatccaaatg agaatgaaat 1380atattgataa ttctgttatc caaatgagag tgaaagataa tgataatttg gagcaaactg 1440
gactaacatt ttcagaaaaa acaatatata tatgtgtatg tgtatgtctg caattatcag 1500gataattcaa ggaaaacaaa tactattgct cgaattgatc atataattat ggaaaatata 1560agaagatgtg aaaacaaagg ccctttataa aatacaaaga cctgaccggg tcaccaggct 1620tgctgaaact gttcaatgac ttcccagggt tttaggccag aaccctcagc ctccttcaag 1680gcctgcctag tctgtctgtg ctcttctctg cagcatcact ggcaccacac ccctccttgc 1740cctgggcaag ccagctcctc tgcccttctt tcagctcttg caagacttcc tcttgttttt 1800gccactgagc ctttgcacat gctgcccttg cttttactta ccctagctac ttattctata 1860gtttttactc aaatcccact ttgggaggta aacctttttt acctcccaga acataatatt 1920ttcatagtat ttggctctaa taaaaccaat tttttttccc agaaaaaaaa aaaaaaaaaa 1980aaaaaaaaaa 1990<210>24<211>969<212>DNA<213>Homo sapiens<400>24ctgccacata aatcctctgc gggagatgat gacaattcac tgctgattaa tgccattctg 60cctttactgt aattagaagg aaataacccc agcaatacaa ggtaatttag caagataagg 120aacccctgct gctacctaaa catccatcta aacaaagatg tttggctttt gaagcaaaga 180gtttggttct taagactgtg ttctttgaca gttaattttc aagaagactg aagactgaat 240tatcattgtt gagaattctc taggtctcag taaccctctg aaccagcagt ttgggtggtc 300gatgcccagc aaataggagt gggtggcctt ttctctggtg tataagattc atctaatttt 360taggaatttt tgtaccattt tccccctcta gaaacacatt tactccccaa taattgtacg 420ggaggtgatc gaggaagaag aaccaagtga aaaatcagag gccacctaca tgaccatgca 480cccagtttgg ccttctctga ggtcagatcg gaacaactca cttgaaaaaa agtcaggtgg 540gggaatgcca aaaacacagc aagccttttg agaagaatgg agagtccctt catctcagca 600gcggtggaga ctctctcctg tgtgtgtcct gggccactct accagtgatt tcagactccc 660gctctcccag ctgtcctcct gtctcattgt ttggtcaata cactgaagat ggagaatttg 720
gagcctggca gagagactgg acagctctgg aggaacaggc ctgctgaggg gaggggagca 780tggacttggc ctctggagtg ggacactggc cctgggaacc aggctgagct gagtggcctc 840aaaccccccg ttggatcaga ccctcctgtg ggcagggttc ttagtggatg agttactggg 900aagaatcaga gataaaaacc aacccaaatc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 960aaaaaaaaa 969<210>25<211>2215<212>DNA<213>Homo sapiens<400>25ttccccggct ctagcaggcc ggcttctctg tccaatgccc acccggagct gggaggagga 60gtctgcgtaa tgtgcgtgtg aagagactgg gggagctggc cggggctcac ggtgtttgac 120ccgtcggtcg tgcgtgagag gaaagggaag gaggaggtcc cgaatagcgg tcgccgaaat 180gttccggtgt ggaggcctgg cggcgggtgc tttgaagcag aagctggtgc ccttggtgcg 240gaccgtgtgc gtccgaagcc cgaggcagag gaaccggctc ccaggcaact tgttccagcg 300atggcatgtt cctctagaac tccagatgac aagacaaatg gctagctctg gtgcatcagg 360gggcaaaatc gataattctg tgttagtcct tattgtgggc ttatcaacag taggagctgg 420tgcctatgcc tacaagacta tgaaagagga tgaaaaaaga tacaatgaaa gaatttcagg 480gttagggctg acaccagaac agaaacagaa aaaggccgcg ttatctgctt cagaaggaga 540ggaagttcct caagacaagg cgccaagtca tgttcctttc ctgctaattg gtggaggcac 600agctgctttt gctgcagcca gatccatccg ggctcgggat cctggggcca gggtactgat 660tgtatctgaa gatcctgagc tgccgtacat gcgacctcct ctttcaaaag aactgtggtt 720ttcagatgac ccaaatgtca caaagacact gcgattcaaa cagtggaatg gaaaagagag 780aagcatatat ttccagccac cttctttcta tgtctctgct caggacctgc ctcatattga 840gaatggtggt gtggctgtcc tcactgggaa gaaggtagta cagctggatg tgagagacaa 900catggtgaaa cttaatgatg gctctcaaat aacctatgaa aagtgcttga ttgcaacagg 960aggtactcca agaagtctgt ctgccattga tagggctgga gcagaggtga agagtagaac 1020
aacgcttttc agaaagattg gagactttag aagcttggag aagatttcac gggaagtcaa 1080atcaattacg attatcggtg ggggcttcct tggtagcgaa ctggcctgtg ctcttggcag 1140aaaggctcga gccttgggca cagaagtgat tcaactcttc cccgagaaag gaaatatggg 1200aaagatcctc cccgaatacc tcagcaactg gaccatggaa aaagtcagac gagagggggt 1260taaggtgatg cccaatgcta ttgtgcaatc cgttggagtc agcagtggca agttacttat 1320caagctgaaa gacggcagga aggtagaaac tgaccacata gtggcagctg tgggcctgga 1380gcccaatgtt gagttggcca agactggtgg cctggaaata gactcagatt ttggtggctt 1440ccgggtaaat gcagagctac aagcacgctc taacatctgg gtggcaggag atgctgcatg 1500cttctacgat ataaagttgg gaaggaggcg ggtagagcac catgatcacg ctgttgtgag 1560tggaagattg gctggagaaa atatgactgg agctgctaag ccgtactggc atcagtcaat 1620gttctggagt gatttgggcc ccgatgttgg ctatgaagct attggtcttg tggacagtag 1680tttgcccaca gttggtgttt ttgcaaaagc aactgcacaa gacaacccca aatctgccac 1740agagcagtca ggaactggta tccgatcaga gagtgagaca gagtccgagg cctcagaaat 1800tactattcct cccagcaccc cggcagttcc acaggctccc gtccaggggg aggactacgg 1860caaaggtgtc atcttctacc tcagggacaa agtggtcgtg gggattgtgc tatggaacat 1920ctttaaccga atgccaatag caaggaagat cattaaggac ggtgagcagc atgaagatct 1980caatgaagta gccaaactat tcaacattca tgaagactga agccccacag tggaattggc 2040aaacccactg cagcccctga gaggaggtcg aatgggtaaa ggagcatttt tttattcagc 2100agactttctc tgtgtatgag tgtgaatgat caagtccttt gtgaatattt tcaactatgt 2160aggtaaattc ttaatgttca catagtgaaa taaattctga ttcttctaaa ttaaa2215<210>26<211>839<212>DNA<213>Homo sapiens<400>26ccctcctgcg atcgcagccc agcgcgtggc gggcgcgcta aaataatgtg attccccggg 60ctgcaggaat tcggcacgag gaaaaaagaa agagaaaggg gaaggaataa gccggaatgc 120
cttttgtgaa caagattcga aacttacaca cttctgccac actttttaaa cgttatttat 180ttatttctta gagacagagt cttgctccgt cacccaggct ggaatgcagg gacctgatct 240tggcttactg caccttggaa ctcctgagct caagcaatcc tgcctcagac tcccaagtag 300ctgagactac aggtgtgagc catttcctcc ggcacatgtt attcaatata aacaagtcac 360tgaaatctgg cacataccca gaagtagaat taagctacac cctttcagat aagaatagta 420aaagaactta ttgaaatatt ccaaagccaa aaatgtggac attgacccca actgcaaaaa 480tacctcagca aattccaaag tcttgaagac ttctgagtag atccccccgt gggtgaggag 540gccaaccaca agcaacactc agtggtagca ggaggtggca ggcgtgaggg caggaagcag 600tgcccctctc agtctcccag cagacctgcc accatgatca gccagccctc tataccaccc 660actggccagc ccatcctgga agatgccaag tggacaagcc agcactcaga cacacagatt 720tgggaaagtg aaaaaccatt tagccctggg gcttgaggca gggaaaggga tgggatgggc 780aagaagtgca tggccatgcc cagtcccagg aaaaaaaaaa aaaaaaaaaa aaaaaaaaa839<210>27<211>615<212>DNA<213>Homo sapiens<400>27gctggacttc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa 60ttcggcacga gctggggcgt agcctccttt ctccagaaag gaaatgatga ccactggatc 120gtcgacacag actacgacac gtatgccgtg cagtactcct gccgcctcct gaacctcgat 180ggcacctgtg ctgacagcta ctccttcgtg ttttcccggg accccaacgg cctgccccca 240gaagcgcaga agattgtaag gcagcggcag gaggagctgt gcctggccag gcagtacagg 300ctgatcgtcc acaacggtta ctgcgatggc agatcagaaa gaaacctttt gtagcaatat 360caagaatcta gtttcatctg agaacttctg attagctctc agtcttcagc tctatttatc 420ttaggagttt aatttgccct tctctcccca tcttccctca gttcccataa aaccttcatt 480acacataaag atacacgtgg gggtcagtga atctgcttgc ctttcctgaa agtttctggg 540gcttaagatt cagaatctga ttcattaaac tatagtcacc ccgtgaaaaa aaaaaaaaaa 600
aaaaaaaaaa aaaaa615<210>28<211>1100<212>DNA<213>Homo sapiens<400>28ggcggccgtt ctacaactag tggatccccc gggctgcagg aattcggcac gagctacagt 60tgaaaccctc acaccacgca ggaagaggtc atcatcatgt cggacgattt cttatggttt 120gaaggcatag ctttccctac tatgggtttc agatccgaaa ccttaagaaa agtacgtgat 180gagttcgtga taagggatga agatgtaata atattgactt accccaaatc aggaacaaac 240tggttggctg agattctctg cctgatgcac tccaaggggg atgccaagtg gatccaatct 300gtgcccatct gggagcgatc accctgggta gagagtgaga ttgggtatac agcactcagt 360gaaacggaga gtccacgttt attctcctcc cacctcccca tccagttatt ccccaagtct 420ttcttcagtt ccaaggccaa ggtgatttat ctcatgagaa atcccagaga tgttttggtg 480tctggttatt ttttctggaa aaacatgaag tttattaaga aaccaaagtc atgggaagaa 540tattttgaat ggttttgtca aggaactgtg ctatatgggt catggtttga ccacattcat 600ggctggatgc ccatgagaga ggagaaaaac ttcctgttac tgagttatga ggagctgaaa 660caggacacag gaagaaccat agagaagatc tgtcaattcc tgggaaagac gttagaaccc 720gaagaactga acttaattct caagaacagc tcctttcaga gcatgaaaga aaacaagatg 780tccaattatt ccctcctgag tgttgattat gtagtggaca aagcacaact tctgagaaaa 840ggtgtatctg gggactggaa aaatcacttc acagtggccc aagctgaaga ctttgataaa 900ttgttccaag agaagatggc agatcttcct cgagagctgt tcccatggga ataacgtcca 960aaacactctg gatcttattt ggagaatgac attgattctc ctgtccttgt acatgtacct 1020gactggggtc ttggtgtaag acttattatt ttatcctgaa accttaatat caaaccctgc 1080tctctaaaaa aaaaaaaaaa 1100<210>29<211>242
<212>DNA<213>Homo sapiens<400>29cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt cggcacgagc 60cgtcatctac tctaccatct ttgcaggcac actcatcaca gcgctaagct cgcactgatt 120ttttacctga gtaggcctag aaataaacat gctagctttt attccagttc taaccaaaaa 180aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 240aa 242<210>30<211>1897<212>DNA<213>Homo sapiens<400>30aaactcagaa aacaattaac tggccagcaa gcatgctaaa agggaaaaga gaatccaaaa 60atttcagtag ctacagtata gaaagatgca actctcataa caaaaaggta agaaaaaaat 120caagaaatgc tttgagaaac aatgacaaat aattaaggca accttaaagc aaaagccaac 180ttgagggcat gccaatctta ttatttgcta tagcctctga ataatttcag ttgattcatg 240ataaatcctt tcaattgact aaaatggctt agtgaaaata aactaatagt gagcttcttt 300cacattatcc ttataaatta aacataatga gaatgaaatt tatagaacat atctttaatc 360tttaaaatac tctatacttt ttgatgtgtc tattggcatg tagaaaaaat tgccatcaag 420taggtaaaaa gccaaaaaaa aatctttttt cagtgaattg cactgccaaa tgaaccatca 480gctaaaaatg aaagactgtt attatcaaat tttaaaataa ctcttggatt cctaaacttc 540tacaggcaaa aacaggttga gacattaact tattccaata gaggatatag tctccttttc 600agatgtggcc aagaaggata ctgaacttca agggaagagc caagattcaa aaagaatgga 660tggaggtcct cctcctatgc agtagaagtt ttactttcaa gaaaatgctt ttcatccaac 720atagaagact cttgcaaata tctgtatttc ttggtttcag aatttttaca taccagtaat 780ggcaatgttc ctcctattct ttccttttct caaaggaagt gtttattgtc ataaatatgt 840ccttgtttct ccattatatc ttaaggatgg agacataacc ttccatttta ttcacatatc 900
tttagactaa gaaaagtgat atgtaaaaca tatttaaaat tgtggacatt aagtgaatat 960tgaggtatct ttgtatcttt cttagtgatg gggcgaatga tgttgaataa aaaaggaagg 1020aaacttaata tctgttacca agatagtaga ttctggtata ttagagtatt agataagact 1080atgtatgaga gtgttgctcc cattatatat ccacctcacc tgactgtagg aatattgtat 1140ttatgtttct tccttttctt aaaaaatggc attctttcta tctgaatact gaaattaacc 1200agacatgtgg agcagagcca gagctgacct accactgctg cagatcatat caagtgagag 1260tgagaaatat ttctgttgca aatcattgag tttgttaaag tcgtttgtta ccacagtaag 1320aactaacaca cccgctcaat ttcaaactca ttttttcaca attcctttta atttgatctt 1380tattttttta ttaatgtgat ttttctccta gtacatatta ctttcaacat ccaatatagt 1440ttatgcattt ataatggtta ttgtgaactg tatgaccatt ttattagatt ataagccata 1500agaagtcagg gattctttca cattttgttt ggtaatgtat ctggagtaca aaaagcaaaa 1560tatggactta aatatttgta gaatgaatga atcatgctgt cataaaactt gtggcaaata 1620aatgctaggg aaaaaatttg gaattaagct catcctgtag aggtaaccta tattaaaaat 1680ttgaaaattc taacacatag caacagttat cacgaatgtt attggtggag ggatagaaaa 1740atagatcaat gaaacagaat aaagaaacca aaaatagatt tttgcttaac cgatttttga 1800aaacaggtgc aaaggtaatg aaatggtagc agcatagcct tttcaaaaaa tggtgctaga 1860agaaatgtac tctagaggca aaaaaaaaaa aaaaaaa1897<210>31<211>499<212>DNA<213>Homo sapiens<400>31cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt cggcacgagg 60acttgctggc cctggggcct tctttgcctt ggcagcgacc cagtgtggca gaatgcaatg 120ttctggtttg gggtctcaag ctaagggctg gtgaggaagg ctgcggaagt ataaatcttc 180aaagtgaata tgtcccaaag gggctggaga gttgcaccaa cctttagggt cttgatttcc 240ctcagtggtt cctacatttc atgtaactgt tttctataat tttttttgca cttttaagag 300
actttttatg taaatgaatt tataggttaa tgaaaaattt ttgtcataac atatatccca 360acttttggtt cagaaaatgt ggccaccatg cccactggga aatgttggga tctccatgaa 420agaggtggct ggatgaaaaa gctcatggct tgaaggtggg aaagaaaaaa aaaaaaaaaa 480aaaaaaaaaa aaaaaaccc499<210>32<211>1075<212>DNA<213>Homo sapiens<400>32ccaacgcggt ggcggccgct ctagaactag tggatccccc gggctgcagg aattcggcac 60gagaaaacta tgctgaggca aaggatgtct tcctgggcat gtttttgtat gaatatgcaa 120gaaggcatcc tgattactct gtcgtgctgc tgctgagact tgccaagaca tatgaaacca 180ctctagagaa gtgctgtgcc gctgcagatc ctcatgaatg ctatgccaaa gtgttcgatg 240aatttaaacc tcttgtggaa gagcctcaga atttaatcaa acaaaattgt gagctttttg 300agcagcttgg agagtacaaa ttccagaatg cgctattagt tcgttacacc aagaaagtac 360cccaagtgtc aactccaact cttgtagagg tctcaagaaa cctaggaaaa gtgggcagca 420aatgttgtaa acatcctgaa gcaaaaagaa tgccctgtgc agaagactat ctatccgtgg 480tcctgaacca gttatgtgtg ttgcatgaga aaacgccagt aagtgacaga gtcaccaaat 540gctgcacaga atccttggtg aacaggcgat catgctttca gctctggaag tcgatgaaac 600atacgttccc aaagagttta atgctgaaac attcaccttt ccatgcagat atatgcacac 660tttctgagaa ggagagacaa atcaagaaac aaactgcact tgttgagctc gtgaaacaca 720agcccaaggc aacaaaagag caactgaaag ctgttatgga tgatttcgca gcttttgtag 780agaagtgctg caaggctgac gataaggaga cctgctttgc cgaggagggt aaaaaacttg 840ttgctgcaag tcaagctgcc ttaggcttat aacatcacat ttaaaagcat ctcagcctac 900catgagaata agagaaagaa aatgaagatc aaaagcttat tcatctgttt ttctttttcg 960ttggtgtaaa gccaacaccc tgtctaaaaa acataaattt ctttaatcat tttgcctctt 1020ttctctgtgc ttcaattaat aaaaaatgga aagaatctaa aaaaaaaaaa aaaaa1075
<210>33<211>852<212>DNA<213>Homo sapiens<400>33ccttaaagct ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccgggc 60tgcaggaatt cggcacaggc gtggttgggg gccatccggg caactcaccc tggacagtca 120gcttgcggaa tcggcagggc cagcatttct gcggggggtc tctagtgaag gagcatggat 180actgactgcc cggcagtgct tctcctcctg ccatatgcct ctcacgggct atgaggtatg 240gttgggcacc ctgttccaga acccacagca tggagagcca agcctacagc gggtcccagt 300accaagatgg tgtgtgggcc ctcaggctcc cagcttgtcc tgctcaagct ggaagatctg 360tgaccctaac cagcgtgtgg ccctgatctg cctgccccct gaatggtatg tggtgcctcc 420agggaccaag tgtgagattg cagctggggt gagaccaaag gtacgggtaa tgacacagtc 480ctaaatgtgg ccttgctgaa tgtcatctcc aaccagagtg taacatcaag caccgaggac 540gtgtgcggga gagtgagatg tgcactgagg gactgttggc ccctgtgggg gcctgtgagg 600gtgactacgg gggcccactt gcctgcttta cccacaactg ctgggtcctg gaaggaatta 660taatccccaa ccgagtatgc gcaaggtccc gctggccagc tgtcttcacg cgtgtctctg 720tgtttgtgga ctggattcac aaggtcatga gactgggtta ggcccagcct tgatgccata 780tgccttgggg aggacaaaac ttcttgtcag acataaagcc atgtttcctc tttaaaaaaa 840aaaaaaaaaa aa 852<210>34<211>694<212>DNA<213>Homo sapiens<400>34ccaccgcggt ggcggccgct ccagaactag tggatccccc gggctgcagg aattcggcac 60gaggtataat agatgtcatg cagccaatcc aaacggcaga tactactggg gtggacagta 120cacctgggac atggcaaagc atggcacaga tgatggtgta gtatggatga attggaaggg 180gtcatggtac tcaatgaaga agatgagtat gaagatcagg cccttcttcc cacagcaata 240
gtccccaata cgtagatttt tgctcttctg tatgtgacaa catttttgta cattatgtta 300ttggaatttt ctttcataca ttatattcct ctaaaactct caagcagacg tgagtgtgac 360tttttgaaaa aagtatagga taaattacat taaaatagca catgattttc ttttgttttc 420ttcatttctc ttgctcacca agaagtaaca aaagtatagt tttgacagag ttggtgttca 480taatttcagt tctagttgat tgcgagaatt ttcaaataag gaagaggggt ctctttatcc 540tggccgtagg aaaaccatga cggaaaggaa aaactgatgt ttaaaagtcc acttttaaaa 600ctatatttat ttatgtagga tctgtcaaag aaaacttcca aaaagattta ttaattaaac 660cagactctgt tgcaataaaa aaaaaaaaaa aaaa 69权利要求
1.一类分离出的在人类肝癌中特异表达的表达序列标签的序列，其包括(a)SEQ ID No.1～SEQ ID No.34所示的序列；(b)SEQ ID No.1～SEQ ID No.34所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.34所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
2.根据权利要求1所述的一类分离出的在人类肝癌中特异表达的表达序列标签的序列，其特征在于所述序列包括具有SEQ ID No.1～SEQ ID No.34所示的序列。
3.一种探针分子，其特征在于所述的探针分子含有权利要求1中所述的序列中约8-100个连续的核苷酸。
全文摘要
本发明公开了一类新的在人类肝癌中特异表达的表达序列标签的序列。利用本发明的在人类肝癌中特异表达的表达序列标签，可以方便的寻找出在人类肝癌中特异表达的相关基因，从而在研究肝癌的致病机理以及开发治疗肝癌的药物中发挥重要作用。
文档编号C12N15/00GK1690201SQ20041001798
公开日2005年11月2日 申请日期2004年4月28日 优先权日2004年4月28日
发明者黄健, 韩泽广 申请人:上海人类基因组研究中心