高起泡性志贺氏菌菌株的制作方法

本申请是申请日为2010年9月28日的中国专利申请201080052643.0“高起泡性志贺氏菌菌株”的分案申请。

本发明属于抗志贺氏菌的免疫法领域。

背景技术：

志贺氏菌属是革兰氏阴性非运动性兼性厌氧菌，其可分为4种血清群：痢疾志贺氏菌(s.dysenteriae)、福氏志贺氏菌(s.flexneri)、鲍氏志贺氏菌(s.boydii)、宋内志贺氏菌(s.sonnei)。它们造成志贺氏菌痢(杆菌性痢疾)。

临床上志贺氏菌痢的标志是急性直肠结肠炎，其伴随有发烧、恶心、食欲减退、脱水、粘液脓性和血性腹泻以及里急后重。志贺氏菌导致的痢疾是地方病，可在发展中国家中导致成百万例的发病。例如，每年约有1.65亿例志贺氏菌痢，其中的99％发生在发展中国家，69％发生在5岁以下的儿童中。志贺氏菌痢造成的发病率和死亡率在发展中国家的儿童中尤其高。

参考文献1中对志贺氏菌疫苗的现有途径进行了综述，这些途径基于活减毒菌株，其用于口服免疫法、与o糖偶联用于注射、用于鼻内的蛋白体(连接有志贺氏菌lps的脑膜炎球菌外膜囊泡)、用于鼻内的侵袭复合物(invaplexes，包含ipab、ipac和lps的志贺氏菌属亚细胞提取物)、以及由具有去毒lps的msbb^-ve菌株制得的核蛋白-核糖体复合物。虽然这些疫苗中的一些已在实地试验中显效，但是没有一种疫苗可提供对抗多种志贺氏菌血清型的保护。

本发明的一个目的是提供在志贺氏菌疫苗制备中进一步提供和改进有用组分，尤其是可提供对抗多种志贺氏菌血清型的保护的疫苗。

技术实现要素：

由于细胞被膜的膨压，志贺氏菌在生长期间自发地释放外膜突泡(bleb)。如参考文献2中所揭示，突泡的释放高度依赖于细菌外膜的结构。本发明人已经采用志贺氏菌的突变株来干扰外膜的结构，该突变株中的tol-pal系统已被破坏。在正常生长期间，这些突变株将大量突泡释放到其培养基中，这些突泡富含免疫原性外膜蛋白，因此这些突泡可被用作免疫原。

由此，本发明提供了表达tola、tolb、tolq、tolr和pal蛋白中不多于4种蛋白质的志贺氏菌。因此，天然的五蛋白tol-pal系统中的至少一种蛋白质是缺失的，导致了细菌在培养基中的生长过程中，与表达全部五种tol-pal蛋白的相同细菌相比，将更多量的外膜突泡释放到培养基中。优选tolr不表达，但可表达其它四种蛋白质。

本发明还提供一种不表达tolr蛋白的志贺氏菌。本发明还提供一种志贺氏菌的δtolr株，例如δtolrδgalu菌株。

本发明还提供一种志贺氏菌，其表达tola、tolb、tolq、tolr和pal蛋白，其中所述tola、tolq、tolr和/或pal蛋白(a)位于细菌的内膜或外膜内，且(b)包含一个或多个氨基酸序列突变，由此使得该细菌与不含所述突变的相同细菌相比，在培养基中生长时释放更大量的外膜突泡。

本发明还提供一种志贺氏菌，该细菌中的tol-pal系统中的一种或多种组分具有修饰，从而使得该细菌与缺乏修饰的相同细菌相比，在培养基中生长时将更大量的外膜突泡释放到培养基中，并且该细菌不表达：(i)天然志贺氏菌的脂多糖和/或(ii)志贺氏菌的肠毒素。

本发明还提供一种制备高起泡性志贺氏菌的方法，所述方法包括修饰编码起始细菌(startingbacterium)的tol-pal系统一种或多种组分的基因的步骤，所述修饰得到所述细菌与起始细菌相比，在培养基中生长时将更大量的外膜突泡释放到培养基中，且其中所述修饰涉及使得起始菌的tola、tolb、tolq、tolr和/或pal基因发生一种或多种突变。该突变步骤可为所述基因的缺失。该方法还可涉及编码合成该菌的脂多糖或肠毒素所需的蛋白质的基因的修饰。

本发明还提供一种分离或获自本发明的细菌的突泡。这些突泡可用作志贺氏菌疫苗的组分。

本发明还提供一种制备志贺氏菌突泡的方法，所述方法包括从包含本发明细菌的培养基中分离所述突泡的步骤，其中本发明细菌已在允许该细菌将突泡释放到所述培养基中的条件下生长。通过该方法制备的突泡可用作志贺氏菌疫苗的组分。

本发明还提供一种包含本发明细菌的培养基，所述细菌已在允许该细菌将突泡释放到所述培养基中的条件下生长。可从该培养基中纯化所述突泡。

本发明还提供一种包含突泡的组合物，所述突泡在本发明细菌的培养过程中释放入培养基。该组合物不含任何活的和/或完整细菌。该组合物和/或其组分可用于志贺氏菌疫苗的制备。

本发明还提供一种包含突泡的组合物，其中所述突泡存在于过滤液中，所述过滤液是将本发明细菌生长的培养基通过0.22μm滤器后获得的。该组合物和/或其组分可用于志贺氏菌疫苗的制备。

本发明还提供一种志贺氏菌突泡，其包含以下的一种或多种(即1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59或60种)：(a)由选自seqidno：8-67中的一种氨基酸序列组成的蛋白质；(b)包含与seqidno：8-67中的一种具有至少j％的相同性的氨基酸序列的蛋白质，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含seqidno：8-67中任一种中至少n个连续氨基酸的片段的蛋白质，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多个)。优选的片段包含来自seqidno：8-67之一的抗原表位。其它优选的片段缺失所述seqidno：的c-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或n-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该seqidno：的至少一个表位。其它片段缺漏一个或多个蛋白质结构域，例如缺失信号肽等。

已确认本发明的突泡中存在60种蛋白质，这些蛋白质对于抗所述突泡制备的血清具有免疫反应性。因此，从突泡分离出的、以纯化形式存在的各种蛋白质可用作免疫原性组分。由此，本发明还提供包含突泡蛋白但不含突泡的免疫原性组合物，所述突泡蛋白包含：(a)氨基酸序列seqidno：8-67中的一种或多种(例如1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59或60种)；(b)与seqidno：8-67之一具有至少j％相同性的氨基酸序列，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含seqidno：8-67中任一种的至少n个连续氨基酸的片段，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多)。优选的片段包含来自seqidno：8-67之一的抗原表位，更优选的片段为免疫原性片段。其它优选的片段缺失seqidno：的c-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或n-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该seqidno：的至少一个抗原表位。其它片段缺漏一个或多个蛋白结构域，例如缺失跨膜结构域、信号肽等。

本发明还提供一种志贺氏菌突泡，其包含以下的一种或多种(即1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128或129种)：(a)由选自seqidno：8-136中的一种氨基酸序列组成的蛋白质；(b)包含与seqidno：8-136中的一种具有至少j％的相同性的氨基酸序列的蛋白质，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含seqidno：8-136中任一种中至少n个连续氨基酸的片段的蛋白质，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多个)。优选的片段包含来自seqidno：8-136之一的抗原表位。其它优选的片段缺失所述seqidno：的c-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或n-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该seqidno：的至少一个抗原表位。其它片段缺漏一个或多个蛋白质结构域，例如缺失信号肽等。

已确认本发明的突泡中存在129种蛋白质，这些蛋白质对于抗所述突泡制备的血清具有免疫反应性。因此，从突泡分离出的、以纯化形式存在的各种蛋白质可用作免疫原性组分。由此，本发明还提供包含突泡蛋白但不含突泡的免疫原性组合物，所述突泡蛋白包含：(a)氨基酸序列seqidno：8-136中的一种或多种(例如1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128或129种)；(b)与seqidno：8-136之一具有至少j％相同性的氨基酸序列，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含seqidno：8-136中任一种的至少n个连续氨基酸的片段的氨基酸序列，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多)。优选的片段包含来自seqidno：8-136之一的抗原表位，更优选的片段为免疫原性片段。其它优选的片段缺失seqidno：的c-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或n-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该seqidno：的至少一个抗原表位。其它片段缺漏一个或多个蛋白结构域，例如缺失跨膜结构域、信号肽等。

在seqidno：8-136中，与福氏志贺氏菌相关的优选亚组是下表1的“亚组1”中所列的seqidno。在seqidno：8-136中，与宋内志贺氏菌相关的优选亚组是下表1的“亚组2”中所列的seqidno。

tol-pal系统

与许多革兰氏阴性菌类似，志贺氏菌天然具有由tola、tolb、tolq、tolr和pal蛋白构成的tol-pal系统。根据本发明，天然tol-pal系统被破坏，从而导致细菌在细菌复制过程中将更大量的外膜突泡释放到其培养基中。可进行各种破坏。

在一些实施方式中，去除五种tol-pal蛋白中的至少一种，例如通过使得编码该蛋白的基因缺失或失活。因此，细菌可表达tola、tolb、tolq、tolr和pal蛋白中的0、1、2、3或4种蛋白质。去除五种蛋白质中的一种可能已足够，在该情况下该细菌仅表达这些蛋白质中的四种。优选去除tolr蛋白，例如通过使得起始菌株的tolr基因失活。由此，该细胞可为tola⁺tolb⁺tolq⁺tolr^-pal⁺。

在一些实施方式中，该细菌表达全部五种tol-pal蛋白，但至少一种蛋白质发生突变而导致高起泡性。例如，tola、tolq、tolr和/或pal蛋白可发生突变，从而使得蛋白质保留其膜定位，但其与其它tol-pal系统成员的相互作用被破坏。由此，该细菌将把作为跨膜蛋白的tola、tolq和tolr保留在内膜中，把作为向周质(periplasm-facing)脂蛋白的pal蛋白保留在外膜中，但tola、tolq、tolr和/或pal蛋白中的至少一种是突变的。

野生型志贺氏菌的tola、tolb、tolq、tolr和pal蛋白的氨基酸序列的例子作为seqidno：1-5示于序列表中。

志贺氏菌

本发明可采用痢疾志贺氏菌(s.dysenteriae)、福氏志贺氏菌(s.flexneri)、鲍氏志贺氏菌(s.boydii)、宋内志贺氏菌(s.sonnei)的任何血清群。

除了具有被破坏的tol-pal系统而使得细菌在细菌复制期间将更大量的外膜突泡释放到其培养基中，本发明的志贺氏菌可有利地包含相对于野生型菌株而言的一种或多种其它改变。这些改变尤其可用于从该细菌中去除在人疫苗中可具有毒性或不为所需的组分。

例如，细菌可不表达天然志贺氏菌的脂多糖(lps)，因此内毒素活性降低。可进行各种修饰以避免天然lps的合成，这些修饰可破坏天然脂质a的结构、寡糖核心或外膜o抗原。例如，参考文献3报道了通过rfe和galu基因失活导致的lps突变体，参考文献4报道了通过yihe、gale、galk、galm和galt失活导致的lps突变体。类似地，参考文献5报道了因rfc、rfal或galu突变导致的lps缺陷。参考文献6报道了因msbbl和msbb2失活导致的lps突变体，其减少了脂质a的酰化作用。如本文所示，可通过htrb的失活产生脂质a酰化减少的另一种lps突变体[7，8]。

优选缺少lps中的o抗原，从而避免血清型特异性的应答。在宋内志贺氏菌中，当毒性质粒被去除时，则缺少o抗原(见下文)。galu基因编码尿苷二磷酸葡萄糖(udp-葡萄糖)焦磷酸化酶，该基因的失活导致所合成的lps不含附着的(attached)o抗原。galu的失活有利于提供不具有尿苷二磷酸葡萄糖焦磷酸化酶活性的志贺氏菌。rfbf和/或rftg基因的失活可用于提供不具有鼠李糖基转移酶活性的志贺氏菌。rfc的失活可用于提供不具有o抗原聚合酶活性的志贺氏菌。rfbf、rfbg和rfc这三种基因都失活可提供有用的菌株。

优选缺少lps中的六-酰化脂质a。失去毒性质粒(见下文)自动导致失去msbb2基因，染色体的msbbl基因可被失活，从而去除肉豆蔻酰基转移酶活性，并在lps中提供五-酰化脂质a。htrb月桂酰基转移酶的失活可提供主要含四-酰化脂质a的志贺氏菌。优选的菌株具有五-或四-酰化lps。

优选菌株的galu和msbbl均失活，且还缺少毒性质粒，由此提供lps为五-酰化且缺少附着的o抗原的菌株。

一些有用的菌株具有五-或四-酰化lps，所述lps包含附着的o抗原。然而更常规而言，优选菌株具有具有五-或四-酰化lps，所述lps缺少附着的o抗原。具有tolr、rfbg和htrb敲除(以及任选的rfbf和/或rfc失活)的福氏志贺氏菌菌株是有用的。一种有用的宋内志贺氏菌菌株具有tolr突变且缺少毒性质粒。

细菌可不表达肠毒素。例如，福氏志贺氏菌菌株(具体为2a菌株)可不表达全部的志贺氏菌肠毒素1(shet-1)亚单位，例如可使得set1a和/或setlb基因失活。痢疾志贺氏菌菌株可不表达全部两种志贺氏菌毒素亚单位，例如可使得stxa和/或stxb基因中的一种或两种失活。志贺氏菌，尤其是宋内志贺氏菌或福氏志贺氏菌，可不表达肠毒素2(shet-2)，例如可使得ospd3基因失活，或缺少毒性质粒。优选的菌株不编码shet-1、shet-2和志贺氏菌毒素。

可通过常规的诱变技术，从野生型或其它起始菌株方便地制备本发明的志贺氏菌，例如参见参考文献9-11。λred重组系统(lambdaredrecombinationsystem)是用于志贺氏菌的尤为有利的系统。可采用各种途径获得基因失活，例如通过其启动子的缺失或突变，通过其起始密码子的缺失或突变，通过导入提早形成终止密码子(prematurestopcodon)，通过缺失整个编码区域，通过敲除等。优选等基因敲除突变体。在所得的志贺氏菌中，缺少编码所需基因的mrna和/或其翻译被抑制(例如抑制为少于野生型水平的1％)。

本发明的志贺氏菌可包含代替失活基因的标记物基因，例如抗生素抗性标记物。其可通过同源重组完成。然而优选使用未标记的缺失(即不引入标记物基因的缺失)。

毒性志贺氏菌菌株具有220kb的质粒，该质粒介导毒性性质。已显示该″毒性质粒″编码涉及志贺氏菌毒性的数个方面的基因，这些方面包括用于靶向上皮细胞的细菌细胞表面配基、侵袭质粒抗原、virf、virg等。本发明的志贺氏菌可具有毒性质粒，但优选其不具有毒性质粒。缺少该质粒可使得菌株在工业化培养中稳定、通过去除毒性因素而减毒(从而提高生产安全性)、破坏脂多糖(o抗原的生物合成基因位于志贺氏菌中的质粒上)、避免存在shet-2肠毒素(由质粒上的ospd3或sen基因编码)，以及避免存在msbb2，该基因是负责脂质a酰化的msbb基因的第二拷贝。

本发明的志贺氏菌可表达一种或多种异源蛋白，例如志贺氏菌中天然不存在的蛋白质。如果该异源蛋白是外膜蛋白，则来自该菌株的突泡可用作将非志贺氏菌抗原递呈到免疫系统的递送系统。

使志贺氏菌生长的培养条件是本领域中熟知的，例如参见参考文献12-14。例如，它们的培养可采用有机氮源(例如氨基酸混合物，如包含ala、arg、asn、asp；可使用酪蛋白氨基酸)，以丙三醇为碳源等等。在培养基中加入l-天冬氨酸尤为有利，其可同时作为氮源和碳源。

可在限铁(iron-limiting)条件下有利地培养本发明的志贺氏菌，已显示这些条件可上调志贺氏菌属中具有免疫原性且高度保守的铁调蛋白。例如，可在诸如去铁灵(desferal)或2，2′-联吡啶基或8-羟基喹啉的化合物存在下培养细菌。在这些条件下，细菌可提高蛋白质(例如fepa外膜受体、大肠杆菌素i受体(cira)和/或铁嗜铁素受体(fhua))的表达。

突泡和高起泡性

本发明的志贺氏菌与其相应的野生型菌株相比，具有高起泡性，即它们把与野生型菌株相比更大量的突泡释放到其培养基中。这些突泡可用作志贺氏菌疫苗的组分。

由电子显微镜测得的突泡直径通常为35-120nm，例如50nm的直径。

突泡在细菌培养过程中自发释放，并可从该培养基中纯化。理想的纯化包括从活的和/或完整的志贺氏菌中分离突泡，例如通过采用过滤器(例如0.22μm的过滤器)的基于大小的过滤，该过滤器允许突泡通过但不允许完整细菌通过，或通过使用低速离心使细胞沉淀而将突泡留在悬液中。

因此，与培养基不同，本发明的含突泡组合物通常基本上不含活的或死的全菌。突泡的大小意味着它们可通过过滤(例如象通常用于过滤灭菌的过滤)简便地与全菌分离。虽然突泡可滤过标准的0.22μm过滤器，它们会很快被其它物质阻塞，因此在使用0.22μm过滤器之前进行滤过一系列具有降低的孔径的过滤器的过滤除菌循序步骤是有利的。之前过滤器的例子包括具有0.8μm、0.45μm等孔径的过滤器。

从培养基中分离自发释放的突泡比涉及故意破坏外膜(例如通过去污剂除了处理或超声作用)以形成外膜小囊泡的方法更为简便。此外，它们基本上不含内膜和细胞质污染物。

本发明的突泡包含脂质和蛋白质。已分析了突泡的蛋白质内容物，它们包括表1中所列和下文所讨论的蛋白质。

志贺氏菌外膜蛋白

表1列出了本发明的志贺氏菌突泡中检测到的129种蛋白质的genbank名称和gi号。这127种蛋白质可从突泡中分离、以纯化的形式用作免疫原性成分。这129种蛋白质中的优选亚组是表中的前60种(“亚组3”)。

可通过多种方式制备多肽，例如通过化学合成(至少部分合成)，通过用蛋白酶消化较长的多肽，通过rna翻译，通过从细胞培养物纯化(例如从重组表达或从志贺氏菌培养物)等等。在大肠杆菌宿主中进行异源表达是优选的表达途径。

本发明的多肽可附着于或固定于固相载体。本发明的多肽可包含可检测标记物，例如放射性标记物、荧光标记物、或生物素标记物。这在免疫分析技术中尤为有用。

多肽可以各种形式存在(例如天然的、融合的、糖基化的、非糖基化的、脂化的、具有二硫桥等等)。多肽优选为志贺氏菌多肽。

优选以基本上纯或基本上分离的形式(即基本上不含其它志贺氏菌或宿主细胞多肽)或基本上分离的形式制备多肽。总体而言，所提供的多肽处于非自然存在环境，例如它们从其天然存在环境中分离。在某些实施方式中，对象肽存在于与对照相比富含该多肽的组合物中。由此，提供了纯化的多肽，其中“纯化的”是指该多肽存在于基本上不含其它表达的多肽的组合物中，且其中“基本上不含”是指该组合物的少于50％，通常少于30％，更通常少于10％是由其它表达的多肽构成的。

术语“多肽”是指任何长度的氨基酸聚合物。该聚合物可为线性或分支的，其可包含修饰的氨基酸，且其可被插入有非氨基酸。该术语还包括已被天然修饰或被干扰的氨基酸聚合物；例如，二硫键形成、糖基化、脂化、酰化、磷酸化或其它操作或修饰，例如与标记组分偶联。在该定义中还包括例如，含一个或多个氨基酸类似物(包括例如，非天然氨基酸等)以及本领域中已知的其它修饰的多肽。多肽可作为单链存在或作为相结合的链存在。

药物组合物

本发明提供了一种药物组合物，其包含：(a)本发明的突泡；以及(b)药学上可接受的载体。本发明还提供一种制备该组合物的方法，所述方法包括将本发明的突泡与药学上可接受的载体混合的步骤。

本发明还提供了一种药物组合物，其包含：(a)如上所定义的不含突泡的免疫原性组合物；以及(b)药学上可接受的载体。

免疫原性组合物可包含药学上可接受的载体，所述载体可为本身不会诱导产生对接受该组合物的患者有害的抗体的任何物质，且在给予时不会产生过度的毒性。药学上可接受的载体可包括液体，例如水、盐水、丙三醇和乙醇。在此类载体中还可存在辅助物质，例如润湿剂或乳化剂，ph缓冲物质等。关于适合的载体的全面讨论可参见参考文献15。

志贺氏菌感染可影响身体的各种区域，因此可将本发明的组合物制成各种形式。例如，可将该组合物制成作为液态溶液或悬浮液的可注射形式。还可制备适于在注射前溶于或悬浮于液体载剂中的固体形式。可将该组合物制成用于局部给药，例如制成软膏、乳膏或粉末。可将该组合物制成用于口服给药，例如制成片剂或胶囊，或制成糖浆(任选经调味)。可将该组合物制成用于肺部给药，例如制成吸入剂，使用细粉或喷雾器。可将该组合物制成栓剂或阴道栓。可将该组合物制成用于经鼻、经耳或经眼给药，例如制成滴剂。

优选该组合物是无菌的。优选其无热原。优选其经缓冲，例如ph为6-8，通常为ph7左右。用于人时，本发明的组合物可为等渗组合物。

免疫原性组合物包含免疫有效量的免疫原，以及所需的任何其它特定组分。“免疫有效量”是指将该量以单剂量或系列中的部分给予个体可有效治疗或预防。该量的变化取决于待治疗个体的健康和生理状况、年龄、待治疗个体的分类群(例如非人灵长类，灵长类等)、个体免疫系统对综合抗体的容量、所需的保护程度、疫苗的制剂、治疗医师对医疗情况的评估、以及其它相关因素。预期该量的范围较宽，该范围可通过常规试验确定。

采用小囊泡疫苗(例如用于脑膜炎球菌)的早先工作为突泡给药提供了药学、剂量学和制剂指导。本发明组合物中突泡的浓度通常为10-500μg/ml，优选25-200μg/ml，更优选约50μg/ml或约100μg/ml(就突泡中的总蛋白而言)。用于注射的剂量体积通常为0.5ml。

该组合物可与其它免疫调节剂联合给药。

可用于本发明组合物(尤其是无突泡组合物)中的佐剂包括但不限于：

a.含无机物组合物

适于用作本发明中的佐剂的含无机物组合物包括：无机盐，例如铝盐和钙盐。本发明包括无机盐，例如氢氧化物(如氢氧化合物)、磷酸盐(例如羟基磷酸盐，正磷酸盐)、硫酸盐等[例如参见参考文献19的第8和9章]，或不同无机化合物的混合物，其中化合物可为任何形式(例如凝胶、结晶、无定形等)，优选吸附。还可将含无机物组合物配制为金属盐颗粒。

被称为“氢氧化铝”的佐剂是常规的氢氧化铝氧化物的盐，其通常至少部分结晶化。氢氧化铝氧化物(可由式alo(oh)表示)可通过红外(ir)光谱与其它铝化合物(例如氢氧化铝al(oh)3)区分开，尤其可通过1070cm^-1处的吸收带和3090-3100cm^-1处的强肩峰的存在来区分[参考文献19第9章]。半峰高处的衍射带宽度(whh)反映出氢氧化铝佐剂的结晶度，结晶差的颗粒由于具有较小的微晶尺寸而显示出较大的谱线增宽。当whh增加，表面积增加，具有较高whh值的佐剂已显示出具有较大的抗原吸附容量。氢氧化铝佐剂通常呈纤维形态(fibrousmorphology，例如在透射电子显微照片中所见)。氢氧化铝佐剂的pi通常为11，即在生理ph下，该佐剂本身具有表面正电荷。已报道氢氧化铝佐剂在ph7.4时的吸附容量为1.8-2.6mg蛋白质/mgal⁺⁺⁺。

称为“磷酸铝”的佐剂通常是羟基磷酸铝，常还含有少量硫酸盐(即羟基磷酸硫酸铝)。其可通过沉淀获得，沉淀期间的反应条件和浓度影响磷酸根取代所述盐中羟基的程度。羟基磷酸盐中po4/al摩尔比通常在0.3-1.2之间。羟基磷酸盐因存在羟基而有别于严格的alpo4。例如，3164cm^-1处的ir谱带(例如在200℃)表明结构性羟基的存在[参考文献19第9章]。

磷酸铝佐剂的po4/al³⁺摩尔比通常为0.3-1.2，优选为0.8-1.2，更优选为0.95±0.1。磷酸铝通常是无定形的，尤其是羟基磷酸盐。典型的佐剂是po4/al摩尔比为0.84-0.92的无定形的羟基磷酸铝，包含0.6mgal³⁺/ml。磷酸铝通常是颗粒状(如在透射电子显微照片上观察到的片状形态)。在任何抗原吸附后，颗粒的典型直径为0.5-20μm(例如约5-10μm)。已报道氢氧化铝佐剂在ph7.4时的吸附容量为0.7-1.5mg蛋白质/mgal⁺⁺⁺。

磷酸铝的零电点(pzc)与磷酸根取代羟基的程度逆相关，这种取代程度可根据用于通过沉淀制备盐的反应条件和反应物浓度而变化。也通过改变溶液中游离磷酸根离子的浓度(更多磷酸根＝更酸性的pzc)或加入缓冲剂(如组氨酸缓冲剂)(使pzc碱性更强)改变pzc。本发明所用的磷酸铝的pzc通常为4.0-7.0，更优选为5.0-6.5，例如约为5.7。

用于制备本发明组合物的铝盐悬浮液可以含有缓冲液(如磷酸盐或组氨酸或tris缓冲液)，但这并不一直是必需的。该悬浮液优选无菌且无热原。悬浮液可含有游离的水性磷酸根离子，如存在浓度为1.0-20mm，优选5-15mm，更优选约10mm。该悬浮液也可含有氯化钠。

在一个实施方式中，佐剂组分包括氢氧化铝和磷酸铝的混合物。在该情况下，磷酸铝的量多于氢氧化铝，例如重量比至少为2∶1，例如≥5∶1、≥6∶1、≥7∶1、≥8∶1、≥9∶1等。

给予患者的组合物中al⁺⁺⁺的浓度优选小于10mg/ml，例如，≤5mg/ml、≤4mg/ml、≤3mg/ml、≤2mg/ml、≤1mg/ml等。优选范围是0.3-1mg/ml。优选最大值是＜0.85mg/剂。

b.油乳剂

适用作本发明的佐剂的油乳剂组合物包括鲨烯-水乳剂，例如mf59[参考文献19的第10章；还可参见参考文献16](5％鲨烯、0.5％吐温80、和0.5％司盘85，用微流化床配制成亚微米颗粒)。也可以使用完全弗氏佐剂(cfa)和不完全弗氏佐剂(ifa)。

已知各种适合的水包油乳剂，它们通常包括至少一种油和至少一种表面活性剂，所述油和表面活性剂是可生物降解(可代谢)和生物相容的。乳剂中油滴的直径通常小于5μm，优选乳剂包含具有亚微米直径的油滴，通过微流化床实现这种小尺寸以提供稳定乳剂。优选尺寸小于220nm的液滴，因为可对其进行过滤灭菌。

本发明可使用油，例如来自动物(如鱼)或植物来源的油。植物油的来源包括坚果、种籽和谷物。最常用的花生油、大豆油、椰子油和橄榄油是坚果油的示例。也可使用获自(例如)霍霍巴豆的霍霍巴油。种籽油包括红花油、棉花籽油、葵花籽油、芝麻籽油等。在谷物类中，最常用的是玉米油，但也可以使用其它谷类的油，如小麦、燕麦、黑麦、稻、画眉草、黑小麦等。可从坚果和种籽油开始，通过水解、分离和酯化合适物质制备甘油和1，2-丙二醇的6-10碳脂肪酸酯，其不是种籽油中天然存在。来自哺乳动物乳汁的脂肪和油类是可代谢的，因此可以用于实施本发明。获得动物来源纯油所必需的分离、纯化、皂化和其它方法的过程为本领域熟知。大多数鱼类含有容易回收的可代谢油。例如，鳕鱼肝油、鲨鱼肝油和鲸油(例如鲸蜡)是可以用于本发明的几种鱼油的示例。通过生化途径用5-碳异戊二烯单位合成许多支链油，其总称为萜类。鲨鱼肝油含有称为鲨烯的支链不饱和萜类化合物，即2，6，10，15，19，23-六甲基-2，6，10，14，18，22-二十四碳六烯。其它优选油是生育酚(见下文)。包含鲨烯的水包油乳剂是特别优选的。可以使用油的混合物。

可通过′hlb′(亲水/亲脂平衡)对表面活性剂分类。本发明优选的表面活性剂的hlb为至少10，优选至少15，更优选至少16。本发明可与表面活性剂一起使用，所述表面活性剂包括但不限于：聚氧乙烯去水山梨糖醇酯表面活性剂(通常称为吐温)，特别是聚山梨酯20和聚山梨酯80；以商品名dowfaxtm出售的环氧乙烷(eo)、环氧丙烷(po)和/或环氧丁烷(bo)的共聚物，如直链ep/po嵌段共聚物；重复的乙氧基(氧-1，2-乙二基)数量不同的辛苯聚醇，特别感兴趣的是辛苯聚醇9(曲通(triton)x-100，或叔辛基苯氧基聚乙氧基乙醇)；(辛基苯氧基)聚乙氧基乙醇(igepalca-630/np-40)；磷脂，如磷脂酰胆碱(卵磷脂)；衍生自十二烷醇、十六烷醇、十八烷醇和油醇的聚氧乙烯脂肪醚(称为苄泽(brij)表面活性剂)，如三乙二醇单月桂基醚(苄泽30)；以及去水山梨糖醇酯(总称为司盘(span))，如去水山梨糖醇三油酸酯(司盘85)和去水山梨糖醇单月桂酸酯。乳剂中包含的优选表面活性剂是吐温80(聚氧乙烯脱水山梨糖醇单油酸酯)、司盘85(脱水山梨糖醇三油酸酯)、卵磷脂和曲通x-100。如上所述，吐温80等去污剂可提供下文实施例所见的热稳定性。

可使用表面活性剂的混合物，如吐温80/司盘85混合物。聚氧乙烯去水山梨糖醇酯(如聚氧乙烯去水山梨糖醇单油酸酯，吐温80)和辛苯聚醇(如叔辛基苯氧基聚乙氧基乙醇，曲通x-100)的组合也适合。另一种有用的组合包含月桂醇聚醚-9(laureth9)加聚氧乙烯去水山梨糖醇酯和/或辛苯聚醇。

表面活性剂的优选量(重量％)为：聚氧乙烯去水山梨糖醇酯(如吐温80)0.01-1％，特别是约0.1％；辛基-或壬基-苯氧基聚氧乙醇(如曲通x-100或曲通系列的其它去污剂)0.001-0.1％，特别是0.005-0.02％；聚氧乙烯醚(如月桂醇聚醚9)0.1-20％，优选0.1-10％，特别是0.1-1％或约0.5％。

可用于本发明的特定水包油乳剂佐剂包括但不限于：

●鲨烯、吐温80和司盘85的亚微米乳剂。所述乳剂的体积组成可以是约5％鲨烯、约0.5％聚山梨酯80和约0.5％司盘85。以重量计，这些比例为4.3％鲨烯、0.5％聚山梨酯80和0.48％司盘85。这种佐剂称为‘mf59’[16-18]，参考文献19的第10章和参考文献20的第12章更详细地描述了该佐剂。mf59乳剂优选包含柠檬酸根离子，如10mm柠檬酸钠缓冲液。

●包含鲨烯、α-生育酚和聚山梨酯80的乳剂。这些乳剂可含有2-10％鲨烯、2-10％生育酚和0.3-3％吐温80，鲨烯：生育酚的重量比优选≤1(例如0.90)，因为这能使乳剂更稳定。鲨烯和吐温80的体积比可以约为5∶2，或者重量比约为11∶5。可通过下述方法制备一种这样的乳剂：将吐温80溶解于pbs得到2％溶液，然后将90ml该溶液与(5gdl-α-生育酚和5ml鲨烯)的混合物混合，然后使该混合物微流体化。所得乳剂可含有亚微米油滴，如平均直径为100-250nm，优选约180nm的亚微米油滴。

●鲨烯、生育酚和曲通去污剂(如曲通x-100)的乳剂。所述乳剂还可包含3d-mpl(见下文)。所述乳剂可包含磷酸盐缓冲液。

●含有聚山梨酯(如聚山梨酯80)、曲通去污剂(如曲通x-100)和生育酚(如琥珀酸α-生育酚)的乳剂。所述乳剂可包含比例为75∶11∶10(例如750μg/ml聚山梨酯80，110μg/ml曲通x-100和100μg/ml的琥珀酸α-生育酚)的这三种成分，且这些浓度可包含由抗原中的这些组分做出的任何贡献。所述乳剂还可包含鲨烯。所述乳剂还可包含3d-mpl(见下文)。水相可包含磷酸盐缓冲液。

●鲨烯、聚山梨酯80和泊洛沙姆401(“普朗尼克(pluronic)^tml121”)的乳剂。所述乳剂可用ph7.4的磷酸盐缓冲盐水配制。该乳剂是一种有用的胞壁酰二肽递送载体，且已与含苏氨酰基-mdp一起在“saf-1”佐剂中使用[21](0.05-1％thr-mdp、5％鲨烷、2.5％普朗尼克l121和0.2％聚山梨酸酯80)。也可不与thr-mdp一起使用，如在“af”佐剂中[22](5％鲨烯、1.25％普朗尼克l121和0.2％聚山梨酸酯80)。优选进行微流体化。

●含有鲨烯、水性溶剂、聚氧乙烯烷基醚亲水性非离子表面活性剂(例如聚氧乙烯(12)鲸蜡硬脂醚)和疏水性非离子表面活性剂(例如脱水山梨糖醇酯或二缩甘露醇酯，例如去水山梨糖醇单油酸酯或“司盘80”)。乳剂优选为热可逆的和/或至少90％的油滴(以体积计)的尺寸小于200nm[23]。乳剂还可包含一种或多种如下物质：醛糖醇；低温防护剂(例如糖，如十二烷基麦芽糖苷和/或蔗糖)；和/或烷基聚糖苷。可冷冻干燥该乳剂。

●含有0.5～50％油、0.1～10％磷脂和0.05～5％非离子表面活性剂的乳剂。如参考文献24所述，优选的磷脂组分是：磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酰甘油、磷脂酸、鞘磷脂和心肌磷脂。亚微米的微滴尺寸是有利的。

●不可代谢的油(例如轻质矿物油)和至少一种表面活性剂(例如卵磷脂、吐温80或司盘80)的亚微米水包油乳剂。可加入添加剂，例如皂树a皂甙(quilasaponin)、胆固醇、皂甙-亲脂性偶合物(例如gpi-0100，描述于参考文献25，通过葡萄糖醛酸的羧基将脂族胺添加到脱乙酰基皂甙而产生)、溴化二甲基双十八烷基铵和/或n，n-双十八烷基-n，n-二(2-羟乙基)丙二胺。

●包含矿物油、非离子亲脂性乙氧基化脂肪醇、非离子亲水表面活性剂(例如乙氧基化脂肪醇和/或聚氧乙烯-聚氧丙烯嵌段共聚物)的乳剂[26]。

●包含矿物油、非离子亲水性乙氧基化脂肪醇、和非离子亲脂性表面活性剂(例如乙氧基化脂肪醇和/或聚氧乙烯-聚氧丙烯嵌段共聚物)的乳剂[26].

●所含皂甙(例如quila或qs21)和固醇(例如胆固醇)结合成螺旋状胶束的乳剂[27]。

通常，在递送给病人时，组合物中的抗原和佐剂是混合的。乳剂可以在生产过程中与抗原混合，或是在递送时将该乳剂与抗原临时混合。因此，在包装或出售的疫苗中，该佐剂和抗原可分开保存，使用时配制成最终制剂。所述抗原通常是水性形式，从而最终通过混合两种液体制备疫苗。用于混合的两种液体的体积比是可变的(例如5∶1至1∶5)，但通常约为1∶1。

c.皂苷制剂[参考文献19的第22章]

皂苷制剂也可以用作本发明的佐剂。皂苷是在许多种类植物的树皮、叶、茎干、根/甚至花中发现的甾醇糖苷和三萜葡萄糖苷的异质群体。已广泛研究了作为佐剂的得自皂皮树(quillaiasaponariamolina)树皮的皂苷。皂苷也可商业化获自丽花菝葜(smilaxornata)(墨西哥菝葜)、满天星(gypsophillapaniculata)(婚纱花)和肥皂草(saponariaofficianalis)(皂根)。皂苷佐剂制剂包括纯化的制剂，如qs21，以及脂质制剂，如iscom。qs21以商标stimulon^tm出售。

已采用hplc和rp-hplc纯化皂苷组合物。已鉴定了用这些技术纯化的特定组分，包括qs7、qs17、qs18、qs21、qh-a、qh-b和qh-c。所述皂苷优选为qs21。制备qs21的方法参见参考文献28。皂苷制剂也可包含甾醇，如胆固醇[29]。

皂苷和胆固醇的组合可用于形成称为免疫刺激复合物(iscom)的独特颗粒(参见参考文献19的第23章；也可参见参考文献30和31)。iscom通常还包含磷脂，如磷脂酰乙醇胺或磷脂酰胆碱。任何已知的皂苷均可用于iscom中。iscom优选包含quila、qha和qhc中的一种或多种。任选地，iscom可不含其它去污剂[32]。

关于基于皂苷的佐剂开发的综述可参见参考文献33和34。

d.细菌或微生物衍生物

适用于本发明的佐剂包括细菌或微生物衍生物，例如肠细菌脂多糖(lps)的无毒衍生物、脂质a衍生物、免疫刺激性寡核苷酸和adp-核糖基化毒素及其脱毒衍生物。

lps的无毒衍生物包括单磷酰脂质a(mpl)和3-o-脱酰基mpl(3dmpl)。3dmpl是3脱-o-酰基单磷酰脂质a与4、5或6条酰化链的混合物。3脱-o-酰基单磷酰脂质a的优选“小颗粒”形式见参考文献35中所述。3dmpl的此类“小颗粒”小到足以在过滤除菌时通过0.22μm的膜[35]。其它无毒lps衍生物包括单磷酰脂质a模拟物，如氨基烷基氨基葡萄糖苷磷酸盐衍生物，例如rc-529[36，37]。

脂质a衍生物包括大肠杆菌的脂质a衍生物，如om-174。例如参考文献38和39中描述了om-174。

适合用作本发明佐剂的免疫刺激性寡核苷酸包括含cpg基序的核苷酸序列(含有通过磷酸键与鸟苷连接的非甲基化胞嘧啶的二核苷酸序列)。含回文结构或聚(dg)序列的双链rna和寡核苷酸也显示具有免疫刺激作用。

cpg可包含核苷酸修饰/类似物，如硫代磷酸酯修饰，可以是双链或单链。参考文献40、41和42公开了可能的类似取代，例如用2′-脱氧-7-脱氮鸟苷取代鸟苷。参考文献43-48中进一步讨论了cpg寡核苷酸的佐剂作用。

cpg序列可能导向tlr9，例如基序gtcgtt或ttcgtt[49]。cpg序列可特异性诱导th1免疫应答，例如cpg-aodn，或更特异地诱导b细胞应答，例如cpg-bodn。参考文献50-52中讨论了cpg-a和cpg-bodn。优选cpg为cpg-aodn。

优选构建cpg寡核苷酸，从而使得5′末端易被受体识别。任选使两个cpg寡核苷酸序列在3′末端连接以形成“免疫聚体(immunomer)”。参见，例如参考文献53-55。

基于免疫刺激性寡核苷酸的特别有用的佐剂被称为ic-31^tm[56-58]。因此，本发明使用的佐剂可以包含(i)和(ii)的混合物：(i)含有至少一个(优选多个)cpi基序(即胞嘧啶与肌苷相连形成二核苷酸)的寡核苷酸(例如15-40个核苷酸)，和(ii)聚阳离子聚合物，如含有至少一个(优选多个)lys-arg-lys三肽序列的寡肽(如5-20个氨基酸)。所述寡核苷酸可以是包含26-聚体序列5′-(ic)13-3′(seqidno：7)的脱氧核苷酸。聚阳离子聚合物可以是含有11-聚体氨基酸序列klklllllklk的肽(seqidno：6)。seqidno：6和7的组合提供了ic-31^tm佐剂。

细菌adp-核糖基化毒素及其去毒衍生物可以用作本发明的佐剂。优选所述蛋白质衍生自大肠杆菌(大肠杆菌不耐热肠毒素“lt”)、霍乱菌(“ct”)或百日咳菌(“pt”)。参考文献59中描述了将脱毒的adp-核糖基化毒素用作粘膜佐剂，参考文献60中描述了将其用作胃肠道外佐剂。所述毒素和类毒素优选全毒素形式，包含a和b亚单位。a亚单位优选含有脱毒突变；b亚单位优选不突变。所述佐剂优选为脱毒的lt突变体，如lt-k63、lt-r72和lt-g192。参考文献61-68中描述了将adp-核糖基化毒素及其脱毒衍生物，尤其是lt-k63和lt-r72用作佐剂。一种有用的ct突变体是ct-e29h[69]。氨基酸取代的编号优选基于参考文献70中提出的adp-核糖基化毒素的a和b亚单位的比对，该参考文献的全部内容特别纳入本文作为参考

e.人免疫调节剂

适合用作本发明佐剂的人免疫调节剂包括细胞因子，如白介素(例如，il-1、il-2、il-4、il-5、il-6、il-7、il-12[71]等)[72]、干扰素(例如干扰素-γ)、巨噬细胞集落刺激因子和肿瘤坏死因子。优选的免疫调节剂是il-12。

f.生物粘着剂和粘膜粘着剂

生物粘着剂和粘膜粘着剂也可以用作本发明的佐剂。合适的生物粘着剂包括酯化透明质酸微球[73]或粘膜粘着剂，如聚乙烯醇、聚乙烯吡咯烷酮、多糖和羧甲基纤维素、聚(丙烯酸)的交联衍生物。壳聚糖及其衍生物也可用作本发明的佐剂[74]。

g.微粒

微粒也可以用作本发明的佐剂。微粒(即直径为～100nm至～150μm，更优选直径～200nm至～30μm，最优选直径～500nm至～10μm的颗粒)由生物可降解的无毒材料(例如，聚(α-羟酸)、聚羟基丁酸、聚原酸酯、聚酐、聚己内酯等)形成，优选聚丙交酯乙交酯共聚物，并任选经处理而具有带负电表面(例如用sds处理)或带正电表面(例如用阳离子去污剂如ctab处理)。

h.脂质体(参考文献19的第13和14章)

适于用作佐剂的脂质体制剂的例子见参考文献75-77所述。

i.咪唑并喹诺酮(imidazoquinolone)化合物

适合用作本发明佐剂的咪唑并喹诺酮化合物的例子包括咪喹莫特及其同系物(例如，″瑞喹莫德3m″)，见参考文献78和79所述。

本发明也可包含以上说明的一种或多种佐剂各方面的组合。例如，可将以下佐剂组合物用于本发明：(1)皂苷和水包油乳剂[80]；(2)皂苷(如qs21)+无毒lps衍生物(如3dmpl)[81]；(3)皂苷(如qs21)+无毒lps衍生物(如3dmpl)+胆固醇；(4)皂苷(如qs21)+3dmpl+il12(任选+固醇)[82]；(5)3dmpl与(例如)qs21和/或水包油乳剂的组合[83]；(6)saf，含有10％鲨烯、0.4％吐温80^tm、5％普朗尼克-嵌段聚合物l121和thr-mdp，微流体化成为亚微米乳剂或涡旋振荡产生粒度较大的乳剂；(7)ribi^tm佐剂系统(ras)(瑞比免疫化学公司(ribiimmunochem))，含有2％鲨烯、0.2％吐温80和一种或多种细菌细胞壁组分，所述组分选自单磷酰脂质a(mpl)、海藻糖二霉菌酸酯(tdm)或细胞壁骨架(cws)，优选mpl+cws(detox^tm)；和(8)一种或多种无机盐(如铝盐)+lps的无毒衍生物(如3dmpl)。

用作免疫刺激剂的其它物质可参见参考文献19的第7章。

可用氢氧化铝佐剂，抗原通常吸附在该盐上。还优选包含鲨烯的水包油乳液，其具有亚微米油滴，尤其是在年长者中。有用的佐剂组合包括th1和th2佐剂的组合，如cpg和铝盐，或瑞喹莫德和铝盐。可使用铝盐和3dmpl的组合。

免疫法

除提供上述免疫原性组合物外，本发明还提供一种在哺乳动物中引起抗体应答的方法，包括将本发明的免疫原性组合物给予所述哺乳动物。所述抗体应答优选地为保护性抗体应答。本发明还提供用于这类方法中的本发明组合物。

本发明还提供了一种保护哺乳动物免受志贺氏菌感染和/或疾病(例如志贺氏菌痢、赖特综合征和/或溶血性尿毒症综合征)的方法，包含将本发明中的免疫原性组合物给予所述哺乳动物。

本发明提供用作药物(例如，用作免疫原性组合物或疫苗)的本发明组合物。本发明还提供本发明的囊泡在制备防止哺乳动物中志贺氏菌感染的药物中的应用，例如用于预防志贺氏菌痢、赖特综合征和/或溶血性尿毒症综合征。还提供本发明的突泡蛋白(如前所述)在制备防止哺乳动物中志贺氏菌感染的无突泡药物中的应用，例如用于预防志贺氏菌痢。

哺乳动物优选为人。所述人可以是成人，或优选是儿童。当预防性使用疫苗时，人优选为儿童(如幼童或婴儿)；当疫苗用于治疗用途时，人优选为成人。准备给予儿童的疫苗也可给予成年人，例如用以评估安全性、剂量、免疫原性等。

所述用途和方法特别适用于预防/治疗疾病，这些疾病包括但并不限于：志贺氏菌痢、赖特综合征和/或溶血性尿毒症综合征。

治疗性措施的功效可以通过在给予本发明中的组合物后对志贺氏菌感染进行监测来测试。可通过监测在施用组合物后针对突泡中免疫原性蛋白或其它抗原的免疫应答来测试预防性治疗的效力。本发明组合物的免疫原性可通过以下方法测定：将其给予受试对象(如12-16个月大的儿童)，然后测定标准血清学参数。这些免疫应答通常在给予该组合物后约4周测定，并与给予该组合物之前测定的值作比较。当给予一剂以上的组合物时，可以进行一次以上的给药后测定。

本发明的组合物通常直接给予患者。可以通过胃肠外注射(例如，皮下、腹膜内、静脉内、肌肉内或给予到组织间隙)，或通过直肠、口服、阴道、局部、透皮、鼻内、眼部、耳部、肺部或其它粘膜给药途径完成直接递送。优选在大腿或上臂进行肌肉内给药。注射可以通过针头(例如皮下针头)进行，但也可以采用无针注射。通常的肌肉内剂量约为0.5ml。

本发明可用来引发全身和/或粘膜免疫。

剂量治疗可采用单剂量方案或多剂量方案。多剂量可以用于初次免疫方案和/或加强免疫方案。初次给药方案之后，可以进行加强给药方案。可以常规地确定初次剂量之间(例如4-16周)以及初次和加强剂量之间的适当时机。

概述

术语“包含”涵盖“含有”以及“由……组成”，例如“包含”x的组合物可仅由x组成或可含有其它物质，例如x+y。

与数值x相关的术语“约”是任选的，其表示(例如)x±10％。

词语“基本上”不排除“完全”，如“基本上不含”y的组合物可能完全不含y。在必要时，词语“基本上”可从本发明的定义中省略。

两条氨基酸序列间的序列相同性百分数表示进行比对时，所比较的两条序列中相同氨基酸的百分数。利用本领域所知软件程序，例如参考文献84的7.7.18部分所描述的软件程序，可进行比对并确定同源性百分数或序列相同性百分数。优选通过史密斯-沃特曼(smith-waterman)同源性搜索算法进行比对，该算法使用仿射缺口搜索(affinegapsearch)，其中缺口开放罚12分，缺口延伸罚2分，blosum矩阵计62分。参考文献85中公开了史密斯-沃特曼同源性搜索算法。

上文使用“gi”编号。gi号或者“基因信息识别号”(geninfoidentifier)是ncbi将序列加入其数据库时，连续对每一序列记录指定的一串数字。gi编号与序列记录登录号没有相似之处。序列更新(如纠正或加入更多注释或信息)后，将接受新gi编号。因此与给定gi编号相关的序列是不变的。

当本发明涉及“表位”时，该表位可以是b细胞表位和/或t细胞表位。可凭经验鉴定此类表位(如使用pepscan[86，87]或相似方法)，或可对其进行预测(如使用詹姆森-沃尔夫抗原性指数(jameson-wolfantigenicindex)[88]、基于矩阵的方法[89]、mapitope[90]、tepitope[91，92]、神经网络[93]、optimer和epimer[94，95]、adept[96]、t位点[97]、亲水性[98]、抗原性指数[99]或参考文献100-101中公开的方法等)。表位是抗体或t细胞受体的抗原结合位点识别并结合的抗原的某部分，它们也称作“抗原决定簇”。

附图说明

图1显示了从培养基中纯化的本发明突泡的电子显微镜照片。

图2显示了来自宋内志贺氏菌δtolrδgalu的突泡的2dsds-page。

图3显示了不同所示菌株突泡的lps(用抗核心抗体染色)。

图4显示了用被免疫小鼠的血清2d分离的突泡蛋白质的免疫斑点印迹。

图5显示了不同条件下生长的细菌的sds-page。

图6显示了突泡中的吸附蛋白。从左到右的泳道为：1)蛋白质分子量标记物，2)ssomb10μg，3)ssomb2μg，4)吸附在明矾上1个月的ssomb10μg。

图7显示了所示菌株的facs数据。

具体实施方式

宋内志贺氏菌突变体的制备

采用λred系统，使得野生型宋内志贺氏菌53g的tolr基因缺失[11，102]。制备用λred质粒转化的感受态细胞，然后通过同源重组，用设计以抗生素抗性基因替换tolr基因的线性片段转化。通过对抗生素的抗性选择已整合该片段的克隆，通过pcr或其它技术验证tolr的缺失。然后可通过在37℃培养新克隆，去除温度敏感性λred质粒。

已确证了在该δtolr突变体中tolr表达的缺少，与原先的野生型分离物相比，已验证该突变体在生长过程中将更多的突泡释放到培养基中。

用类似的途径使得galu基因缺失，以提供δgalu单突变体和atolrδgalu双突变体。经验证突变体释放的突泡具有缺少o抗原的缺陷型lps。

用相同的方法制备带有修饰lps的δtolrδmsbb双突变株。

还从δtolr和δtolrδmsbb菌株中去除了毒性质粒。

福氏志贺氏菌突变体的制备

如上针对宋内志贺氏菌所述，采用λred系统使得福氏志贺氏菌的tolr基因缺失。福氏志贺氏菌中o抗原的生物合成通过包含部分rfbf和rfc以及完整rfbg基因的染色体片段的缺失(该缺失导致这三种基因全部失活)而被彻底破坏。采用λred系统产生缺失，并缩写为δrfbg。

以相同的方式产生atolrδrfbg双突变体。

以相同的方式产生包含修饰的lps的δtolrδhtrb双突变体。

已从这些菌株中去除了毒性质粒。

突泡的纯化

在以下条件下进行双突变体δtolrδgalu菌株的发酵：ph7.1，37℃，通过受控的搅拌和设置最大充气量将溶氧保持在30％的饱和度。通过加入4m氢氧化铵控制ph。通过在发酵过程中加入10％ppg控制泡沫。培养基由以下组分组成：kh2po45g/l，k2hpo420g/l和酵母提取物30g/l。采用高压灭菌对培养基进行灭菌后，加入甘油15g/l和mgso42mm，然后进行接种。培养接种物为发酵罐体积的5％。发酵过程进行约13个小时，以600nm下的光学密度确定细胞浓度。

采用以下所定义的培养基进行宋内志贺氏菌δtolrδmsbb双突变株的发酵过程：甘油30g/1，kh2po413.3g/1，(nh4)2hpo44g/1，mgso4·7h2o(1m)2ml，柠檬酸1.7g/1，cocl2·6h2o2.5mg/1，mncl2·4h2o15mg/1，cucl2·2h2o1.5mg/1，h3bo33mg/1，na2moo4·2h2o2.5mg/1，zn(ch3coo)2·2h2o13mg/1，柠檬酸铁2μm，硫胺素50mg/1，烟酸10mg/1，l-天冬氨酸2.5g/l。

采用两个连续tff(正切流动过滤)步骤纯化在发酵液中产生的小囊泡：以0.22μm进行微过滤，然后以0.1μm进行第二次微过滤。在第一次过滤步骤中，通过经0.22μm孔径过滤盒的tff从生物质中分离出小囊泡。先将该生物质浓缩4倍，用pbs进行5次透析过滤步骤后，在过滤液中收集小囊泡。在第二次过滤步骤中，使得从0.22μmtff获得的过滤液进一步经0.1μm截留过滤盒进行微过滤，以从溶解蛋白质中纯化小囊泡。小囊泡不能通过过滤盒。经过5次透析过滤步骤后，收集含有小囊泡的保留物。

用tem观察最终纯化产物(图1)。突泡具有约50nm直径的均一尺寸。

在与培养宋内志贺氏菌δtolrδgalu相同的酵母提取物培养基中培养各种菌株，然后以相同的方式纯化来自福氏志贺氏菌突变体的突泡。

突泡的表征

通过蛋白质组方法证实了突泡为基本上纯的外膜。与通过破坏外膜获得的常规外膜囊泡(omv)不同，所述突泡保留亲脂蛋白且基本上不含细胞质和内膜组分，.

用去污剂使得来自宋内志贺氏菌和福氏志贺氏菌菌株的突泡变性，并用lc-ms/ms方法鉴别蛋白质。或者，用sdspage电泳或2d凝胶电泳分离突泡(图2)。从凝胶上切取可见条带和斑点，通过蛋白质质量指纹分析鉴定蛋白质。用密度计分析sda-page条带或来自2d凝胶的斑点研究不同蛋白质的相对量。

图3显示了δgalu突变株与野生型志贺氏菌和大肠杆菌(所有菌株均为δtolr背景)相比的lps迁移率的改变。

采用基于表面消化的第二蛋白质组方法来表征膜蛋白的暴露部分。鉴定了与经突泡免疫的小鼠的血清反应的一系列蛋白质，已发现其中有多种蛋白质在许多菌株中是保守的。对于大部分完整外膜蛋白所知甚少。如下研究突泡的表在蛋白质组(surfome)：用蛋白酶处理，回收，然后通过lc-ms/ms鉴定释放的肽。由于突泡应代表了活的整菌细胞的表面，该图谱可代表宋内志贺氏菌表面上暴露的蛋白质。

通过这些方法和其它方法，在突泡中发现了表1中所列的129种蛋白质。

突泡的免疫原性

如图4所示，用来自δtolrδgalu菌株的突泡免疫的小鼠产生的血清与该突泡的2d凝胶发生反应。因此，该突泡具有免疫原性。

小鼠接受2μg或10μg宋内志贺氏菌δtolrδgalu突泡(以总蛋白质为基准测定)，加或不加佐剂(氢氧化铝或完全弗氏佐剂)。进行经典的elisa方法以分析获自免疫研究的血清中的igg的生产。所有小鼠组的血清均显示突泡特异性igg的高水平生产。单独使用突泡或与佐剂联用时，未检测到igg生产中的显著差异。用2μg的低剂量免疫的组与用10μg免疫的组显示出相同的突泡特异性igg水平，这表明可采用低剂量疫苗，即每美元更多剂量。来自其它宋内志贺氏菌和福氏志贺氏菌菌株的突泡也类似地具有免疫原性。

测定抗突泡产生的血清与三种不同菌的反应性：宋内志贺氏菌g53、福氏志贺氏菌2a2457t或福氏志贺氏菌5m90t。然后用标记的二抗对样品进行染色，并通过流式细胞法进行分析。如图7中所示，宋内志贺氏菌和福氏志贺氏菌菌株与血清发生交叉反应。

因此，该突泡方法在产生有效的低成本疫苗中具有很大的潜力，且可扩展到不同志贺氏菌菌株以产生广谱疫苗。

突泡吸附

将突泡与氢氧化铝(2mg/ml)组合进行吸附。将吸附材料储存在4℃下1天、1周或1个月。1天后突泡完全吸附，且甚至在1个月后都未显现出解吸附迹象(图6)。

限铁生长

图5显示在不同条件下生长的志贺氏菌表达的蛋白质。在第4泳道中，细菌在200μmfeso4的存在下生长，在第5泳道中，培养物包含200μm联吡啶(dypiridyl)。插入的小图显示在限铁条件下三种蛋白质上调。这三种蛋白质鉴定为fepa外膜受体(gi74311118)、大肠杆菌素i受体(gi74312677)和推定铁嗜铁素受体(gi74313972)。这些蛋白质在志贺氏菌属和肠杆菌科中非常保守，并具有潜在的高免疫原性。因此，在限铁条件下生长的志贺氏菌能导致突泡的释放，该突泡与正常生长条件下产生的突泡相比富含这些蛋白质。

应理解，仅以举例的方式描述了本发明，可对之进行修改而仍在本发明的范围和构思内。

表1

seqidno：70、71、73、74、76、111、112、114-129和131-135鉴别自宋内志贺氏菌δtolr突泡。seqidno：8-15和17-58鉴别自宋内志贺氏菌δtolrδgalu突泡。seqidno：83、94、97和107鉴别自福氏志贺氏菌δtolr突泡。seqidno：68、69、72、75、77-82、84-93、95、96、98-106、108-10、113、130和136鉴别自福氏志贺氏菌δtolrδrfbg突泡。seqidno：60-67鉴别自宋内志贺氏菌的表面消化物。

亚组1：seqidno：68、69、72、75、77-110、113、130和136。

亚组2：seqidno：8-15、17-58、60-67、70、71、73、74、76、111、112、114-129和131-135。

亚组3：seqidno：1-60。

nb：seqidno：18与seqidno：5相同；seqidno：33与seqidno：2相同；seqidno：9和16相关(约97％相同性)；seqidno：23和59相关(约98％相同性)。

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[45]wo98/40100.

[46]us6,207,646.

[47]us6,239,116.

[48]us6,429,199.

[49]kandimalla等，(2003)biochemicalsocietytransactiins31(part3)：654-658.

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[60]wo98/42375.

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序列表

<110>全球健康诺华疫苗学院有限公司

(novartisvaccinesinstituteforglobalhealths.r.l.)

<120>高起泡性志贺氏菌菌株

<130>p053584ep

<140>pct/ib2010/002582

<141>2010-09-28

<150>gb-0917002.8

<151>2009-09-28

<160>136

<170>seqwin2010,1.0版

<210>1

<211>413

<212>prt

<213>福氏志贺氏菌(shigellaflexneri)

<400>1

metserlysalathrgluglnasnasplysleulysargalaileile

151015

ileseralavalleuhisvalileleuphealaalaleuiletrpser

202530

serpheaspgluasnileglualaseralaglyglyglyglyglyser

354045

serileaspalavalmetvalaspserglyalavalvalgluglntyr

505560

lysargmetglnserglngluserseralalysargseraspglugln

65707580

arglysmetlysgluglnglnalaalaglugluleuargglulysgln

859095

alaalagluglngluargleulysglnleuglulysgluargleuala

100105110

alaglngluglnlyslysglnalagluglualaalalysglnalaglu

115120125

leulysglnlysglnalagluvalalaalaalalysalaalaalaasp

130135140

alalysalaalagluglualaalalyslysalaalaalaaspalalys

145150155160

lyslysalaglualaglualaalalysalaalaalaglualaglnlys

165170175

lysalagluvalalaalaalaalaleulyslyslysalaglualaala

180185190

glualaalaalaalaglualaarglyslysalaalathrglualaala

195200205

glulysalalysalaglualaglulyslysalaalaalaglulysala

210215220

alaalaasplyslysalaalaalaglulysalaalaalaasplyslys

225230235240

alaalaglulysalaalaalaasplysalaalaalaasplyslysala

245250255

alaalaglulysalaalaalaasplyslysalaalaalaalalysala

260265270

alaalaglulysalaalaalaalalysalaalaalaglualaaspasp

275280285

ilepheglygluleuserserglylysasnalaprolysthrglygly

290295300

glyalalysglyasnasnalaserproalaglyserglyasnthrlys

305310315320

asnasnglyalaserglyalaaspileasnasntyralaglyglnile

325330335

lysseralailegluserlysphetyraspalasersertyralagly

340345350

lysthrcysthrleuargilelysleualaproaspglymetleuleu

355360365

aspilelysprogluglyglyaspproalaleucysglnalaalaleu

370375380

alaalaalalysleualalysileprolysproproserglnalaval

385390395400

tyrgluvalphelysasnalaproleuaspphelyspro

405410

<210>2

<211>430

<212>prt

<213>福氏志贺氏菌

<400>2

metlysglnalaleuargvalalapheglypheleuileleutrpala

151015

servalleuhisalagluvalargilevalileaspserglyvalasp

202530

serglyargproileglyvalvalpropheglntrpalaglyprogly

354045

alaalaprogluaspileglyglyilevalalaalaaspleuargasn

505560

serglylyspheasnproleuaspargalaargleuproglnglnpro

65707580

glyseralaglngluvalglnproalaalatrpseralaleuglyile

859095

aspalavalvalvalglyglnvalthrproasnproaspglysertyr

100105110

asnvalalatyrglnleuvalaspthrglyglyalaproglythrval

115120125

leualaglnasnsertyrlysvalasnlysglntrpleuargtyrala

130135140

glyhisthralaseraspgluvalpheglulysleuthrglyilelys

145150155160

glyalapheargthrargilealatyrvalvalglnthrasnglygly

165170175

glnpheprotyrgluleuargvalserasptyraspglytyrasngln

180185190

phevalvalhisargserproglnproleumetserproalatrpser

195200205

proaspglyserlysleualatyrvalthrphegluserglyargser

210215220

alaleuvalileglnthrleualaasnglyalavalargglnvalala

225230235240

serpheproarghisasnglyalaproalapheserproaspglyser

245250255

lysleualaphealaleuserlysthrglyserleuasnleutyrval

260265270

metaspleualaserglyglnileargglnvalthraspglyargser

275280285

asnasnthrgluprothrtrppheproaspserglnasnleualaphe

290295300

thrseraspglnalaglyargproglnvaltyrlysvalasnileasn

305310315320

glyglyalaproglnargilethrtrpgluglyserglnasnglnasp

325330335

alaaspvalserseraspglylysphemetvalmetvalserserasn

340345350

glyglyglnglnhisilealalysglnaspleualathrglyglyval

355360365

glnvalleuserserthrpheleuaspgluthrproserleualapro

370375380

asnglythrmetvaliletyrserserserglnglymetglyserval

385390395400

leuasnleuvalserthraspglyargphelysalaargleuproala

405410415

thraspglyglnvallyspheproalatrpserprotyrleu

420425430

<210>3

<211>230

<212>prt

<213>福氏志贺氏菌

<400>3

metthraspmetasnileleuaspleupheleulysalaserleuleu

151015

vallysleuilemetleuileleuileglypheserilealasertrp

202530

alaileileileglnargthrargileleuasnalaalaalaargglu

354045

alaglualaphegluasplysphetrpserglyilegluleuserarg

505560

leutyrglngluserglnglylysargaspasnleuthrglyserglu

65707580

glnilephetyrserglyphelysgluphevalargleuhisargala

859095

asnserhisalaproglualavalvalgluglyalaserargalamet

100105110

argilesermetasnarggluleugluasnleugluthrhisilepro

115120125

pheleuglythrvalglyserileserprotyrileglyleuphegly

130135140

thrvaltrpglyilemethisalapheilealaleuglyalavallys

145150155160

glnalathrleuglnmetvalalaproglyilealaglualaleuile

165170175

alathralaileglyleuphealaalaileproalavalmetalatyr

180185190

asnargleuasnglnargvalasnlysleugluleuasntyraspasn

195200205

phemetglugluphethralaileleuhisargglnalaphethrval

210215220

sergluserasnlysgly

225230

<210>4

<211>142

<212>prt

<213>福氏志贺氏菌

<400>4

metalaargalaargglyargglyargargaspleulyssergluile

151015

asnilevalproleuleuaspvalleuleuvalleuleuleuilephe

202530

metalathralaproileilethrglnservalgluvalaspleupro

354045

aspalathrgluserglnalavalserserasnaspasnproproval

505560

ilevalgluvalserglyileglyglntyrthrvalvalvalglulys

65707580

aspargleugluargleuproprogluglnvalvalalagluvalser

859095

serargphelysalaasnprolysthrvalpheleuileglyglyala

100105110

lysaspvalprotyraspgluileilelysalaleuasnleuleuhis

115120125

seralaglyvallysservalglyleumetthrglnproile

130135140

<210>5

<211>173

<212>prt

<213>福氏志贺氏菌

<400>5

metglnleuasnlysvalleulysglyleumetilealaleuproval

151015

metalailealaalacysserserasnlysasnalaserasnaspgly

202530

sergluglymetleuglyalaglythrglymetaspalaasnglygly

354045

asnglyasnmetserserglugluglnalaargleuglnmetglngln

505560

leuglnglnasnasnilevaltyrpheaspleuasplystyraspile

65707580

argseraspphealaglnmetleuaspalahisalaasnpheleuarg

859095

serasnprosertyrlysvalthrvalgluglyhisalaaspgluarg

100105110

glythrproglutyrasnileserleuglygluargargalaasnala

115120125

vallysmettyrleuglnglylysglyvalseralaaspglnileser

130135140

ilevalsertyrglylysglulysproalavalleuglyhisaspglu

145150155160

alaalatyrserlysasnargargalavalleuvaltyr

165170

<210>6

<211>11

<212>prt

<213>人工序列

<220>

<223>阳离子肽

<400>6

lysleulysleuleuleuleuleulysleulys

1510

<210>7

<211>26

<212>dna

<213>人工序列

<220>

<223>免疫刺激性寡核苷酸

<220>

<221>修饰的碱基

<222>1,3,5,7,9,11,13,15,17,19,21,23,25

<223>'n'为'i'(肌苷)

<400>7

ncncncncncncncncncncncncnc26

<210>8

<211>493

<212>prt

<213>福氏志贺氏菌

<400>8

metlyslysleuleuproileleuileglyleuserleuserglyphe

151015

serserleuserglnalagluasnleumetglnvaltyrglnglnala

202530

argleuserasnprogluleuarglysseralaalaaspargaspala

354045

alapheglulysileasnglualaargserproleuleuproglnleu

505560

glyleuglyalaasptyrthrtyrserasnglytyrargaspalaasn

65707580

glyileasnserasnalathrseralaserleuglnleuthrglnser

859095

ilepheaspmetserlystrpargalaleuthrleuglnglulysala

100105110

alaglyileglnaspvalthrtyrglnthraspglnglnthrleuile

115120125

leuasnthralathralatyrpheasnvalleuasnalaileaspval

130135140

leusertyrthrglnalaglnlysglualailetyrargglnleuasp

145150155160

glnthrthrglnargpheasnvalglyleuvalalailethraspval

165170175

glnasnalaargalaglntyraspthrvalleualaasngluvalthr

180185190

alaargasnasnleuaspasnalavalgluglnleuargglnilethr

195200205

glyasntyrtyrprogluleualaalaleuasnvalgluasnphelys

210215220

thrasplysproglnprovalasnalaleuleulysglualaglulys

225230235240

argasnleuserleuleuglnalaargleuserglnaspleugluarg

245250255

gluglnileargglnalaglnaspglyhisleuprothrleuaspleu

260265270

thralaserthrglyileseraspthrsertyrserglyserlysthr

275280285

argglyalaalaglythrglntyraspaspserasnmetglyglnasn

290295300

lysvalglyleuserpheserleuproiletyrglnglyglymetval

305310315320

asnserglnvallysglnalaglntyrasnphevalglyalaserglu

325330335

glnleugluseralahisargservalvalglnthrvalargserser

340345350

pheasnasnileasnalaserileserserileasnalatyrlysgln

355360365

alavalvalseralaglnserserleuaspalametglualaglytyr

370375380

servalglythrargthrilevalaspvalleuaspalathrthrthr

385390395400

leutyrasnalalysglngluleualaasnalaargtyrasntyrleu

405410415

ileasnglnleuasnilelysseralaleuglythrleuasnglugln

420425430

aspleuleualaleuasnasnalaleuserlysprovalserthrasn

435440445

progluasnvalalaproglnthrprogluglnasnalailealaasp

450455460

glytyralaproaspserproalaprovalvalglnglnthrserala

465470475480

argthrthrthrserasnglyhisasnpropheargasn

485490

<210>9

<211>368

<212>prt

<213>宋内志贺氏菌(shigellasonnei)

<400>9

metlysvallysvalleuserleuleuvalproalaleuleuvalala

151015

glyalaalaasnalaalagluvaltyrasnlysaspglyasnlysleu

202530

aspleutyrglylysvalaspglyleuhistyrpheseraspasnlys

354045

sergluaspglyaspglnthrtyrvalargleuglyphelysglyval

505560

thrglnvalthraspglnleuthrglytyrglyglntrpglutyrgln

65707580

ileglnglyasnthrsergluaspasnlysgluasnsertrpthrarg

859095

valalaphealaglyleulyspheglnaspvalglyserpheasptyr

100105110

glyargasntyrglyvalvaltyraspvalthrsertrpthraspval

115120125

leuproglupheglyglyaspthrtyrglyseraspasnphemetgln

130135140

glnargglyasnglyphealathrtyrargasnthraspphephegly

145150155160

leuvalaspglyleuasnphealavalglntyrglnglylysasngly

165170175

servalserglygluglymetthrasnasnglyargglyalaleuarg

180185190

glnasnglyaspglyvalglyglyserilethrtyrasptyrglugly

195200205

pheglyileglyglyalaileserserserlysargthraspaspgln

210215220

asnserproleutyrileglyasnglyaspargalagluthrtyrthr

225230235240

glyglyleulystyraspalaasnasniletyrleualaalaglntyr

245250255

thrglnthrtyrasnalathrargvalglyserleuglytrpalaasn

260265270

lysalaglnasnpheglualavalalaglntyrglnpheaspphegly

275280285

leuargproservalalatyrleuglnserlysglylysasnleugly

290295300

thrilealaglyargasntyraspaspgluaspileleulystyrval

305310315320

aspvalglyalathrtyrtyrpheasnlysasnmetserthrtyrval

325330335

asptyrlysileasnleuleuaspaspasnglnphethrargaspala

340345350

glyileasnthraspasnilevalalaleuglyleuvaltyrglnphe

355360365

<210>10

<211>346

<212>prt

<213>宋内志贺氏菌

<400>10

metlyslysthralailealailealavalalaleualaglypheala

151015

thrvalalaglnalaalaprolysaspasnthrtrptyrthrglyala

202530

lysleuglytrpserglntyrhisaspthrglypheileasnasnasn

354045

glyprothrhisgluasnglnleuglyalaglyalapheglyglytyr

505560

glnvalasnprotyrvalglypheglumetglytyrasptrpleugly

65707580

argmetprotyrlysglyservalgluasnglyalatyrlysalagln

859095

glyvalglnleuthralalysleuglytyrproilethraspaspleu

100105110

aspiletyrthrargleuglyglymetvaltrpargalaaspthrlys

115120125

serasnvaltyrglylysasnhisaspthrglyvalserprovalphe

130135140

alaglyglyvalglutyralailethrprogluilealathrargleu

145150155160

glutyrglntrpthrasnasnileglyaspalahisthrileglythr

165170175

argproaspasnglymetleuserleuglyvalsertyrargphegly

180185190

glnglyglualaalaprovalvalalaproalaproalaproalapro

195200205

gluvalglnthrlyshisphethrleulysseraspvalleupheasn

210215220

pheasnlysalathrleulysprogluglyglnalaalaleuaspgln

225230235240

leutyrserglnleuserasnleuaspprolysaspglyservalval

245250255

valleuglytyrthraspargileglyseraspalatyrasnglngly

260265270

leusergluargargalaglnservalvalasptyrleuileserlys

275280285

glyileproalaasplysileseralaargglymetglygluserasn

290295300

provalthrglyasnthrcysaspasnvallysglnargalaalaleu

305310315320

ileaspcysleualaproaspargargvalgluilegluvallysgly

325330335

ilelysaspvalvalthrglnproglnala

340345

<210>11

<211>407

<212>prt

<213>福氏志贺氏菌

<400>11

metserileaspphethrproglyileileasnthrtyrhisglyasp

151015

iletyrasncysthrthrasnthraspasnvallysthrproaspthr

202530

prolystrpprocysaspasntrpglugluglnglnproileasnser

354045

thrpheserglygluglytyrasnprogluglnpheaspleualagln

505560

glnglnleuglnglnileasnalacyshisthrasnthrthrtyrthr

65707580

asnalaasptyrserlysvalvalvalglnleuvalserleuileasn

859095

asnilegluthrileserserthrproleuthrglnglnthrglnser

100105110

ileleuasnglnilehisasnileargtyrglulysasplysaspser

115120125

valcysglnileilevalvalvalasnprogluproasnasnproile

130135140

ilethrlysileleuvalglugluglyileprogluargpheserval

145150155160

glnthrvalserseraspasnlysasnphealaglyglnargalaasp

165170175

leuprothraspileargaspileglnserleutyrleulysmetala

180185190

lysleutyrilegluhisserlysasngluasnargmetglualaleu

195200205

alaglycysasppheileasppheasnmetthrglyglnaspmetser

210215220

lysleuvalleuthrleuserlysphetyrphegluaspleuleuasn

225230235240

ileasnphethraspalaasnleupheasnthrilepheserhislys

245250255

gluasnproileprolysleuhislystyrgluglnhisleuasplys

260265270

glnileasnglyleupheserthrleuleuthrileasnaspasnser

275280285

leuargalalysalagluilealaserargileileasppheleuglu

290295300

alalysvalvalasnleuserpheaspaspileleulystyrlysgln

305310315320

gluphelyslysilecystyrlysglnleuglngluphethrthrpro

325330335

serleutyrasnlysileglnlystrpalathrmetserlysasnglu

340345350

pheilealaphehistyrgluthrleuglnproglulysilesertyr

355360365

prophetyrleulysargaspleuproasnglulysaspileasntyr

370375380

glyvalgluilegluileproserglylysargileargleuserasn

385390395400

histyrglnasnileilepro

405

<210>12

<211>171

<212>prt

<213>福氏志贺氏菌

<400>12

metlyslysilealacysleuseralaleualaalavalleualaphe

151015

thralaglythrservalalaalathrserthrvalthrglyglytyr

202530

alaglnseraspalaglnglyglnmetasnlysmetglyglypheasn

354045

leulystyrargtyrglugluaspasnserproleuglyvalilegly

505560

serphethrtyrthrglulysserargthralaserserglyasptyr

65707580

asnlysasnglntyrtyrglyilethralaglyproalatyrargile

859095

asnasptrpalaseriletyrglyvalvalglyvalglytyrglylys

100105110

pheglnthrthrglutyrprothrtyrlyshisaspthrserasptyr

115120125

glyphesertyrglyalaglyleuglnpheasnprometgluasnval

130135140

alaleuaspphesertyrgluglnserargileargservalaspval

145150155160

glythrtrpilealaglyvalglytyrargphe

165170

<210>13

<211>155

<212>prt

<213>福氏志贺氏菌

<400>13

metilelysargvalleuvalvalsermetvalglyleuserleuval

151015

glycysvalasnasnaspthrleuserglyaspvaltyrthralaser

202530

glualalysglnvalglnasnvalsertyrglythrilevalasnval

354045

argprovalglnileglnglyglyaspaspserasnvalileglyala

505560

ileglyglyalavalleuglyglypheleuglyasnthrvalglygly

65707580

glythrglyargserleualathralaalaglyalavalalaglygly

859095

valalaglyglnglyvalglnseralametasnlysthrglnglyval

100105110

gluleugluilearglysaspaspglyasnthrilemetvalvalgln

115120125

lysglnglyasnthrargpheserproglyglnargvalvalleuala

130135140

serasnglyserglnvalthrvalserproarg

145150155

<210>14

<211>207

<212>prt

<213>福氏志贺氏菌

<400>14

metproleuproasppheargleuileargleuleuproleualaala

151015

leuvalleuthralacysservalthrthrprolysglyproglylys

202530

serproaspserproglntrpargglnhisglnglnaspvalargasn

354045

leuasnglntyrglnthrargglyalaphealatyrileseraspgln

505560

glnlysvaltyralaargphephetrpglnglnthrglyglnasparg

65707580

tyrargleuleuleuthrasnproleuglyserthrgluleugluleu

859095

asnalaglnproglyasnvalglnleuvalaspasnlysglyglnarg

100105110

tyrthralaaspaspalagluglumetileglylysleuthrglymet

115120125

proileproleuasnserleuargglntrpileleuglyleuprogly

130135140

aspalathrasptyrlysleuaspaspglntyrargleusergluile

145150155160

thrtyrserglnasnglylysasntrplysvalvaltyrglyglytyr

165170175

aspthrlysthrglnproalametproalaasnmetgluleuthrasp

180185190

glyglyglnargilelysleulysmetaspasntrpilevallys

195200205

<210>15

<211>810

<212>prt

<213>福氏志贺氏菌

<400>15

metalametlyslysleuleuilealaserleuleupheserserala

151015

thrvaltyrglyalagluglyphevalvallysaspilehispheglu

202530

glyleuglnargvalalavalglyalaalaleuleusermetproval

354045

argthrglyaspthrvalasnaspgluaspileserasnthrilearg

505560

alaleuphealathrglyasnphegluaspvalargvalleuargasp

65707580

glyaspthrleuleuvalglnvallysgluargprothrilealaser

859095

ilethrpheserglyasnlysservallysaspaspmetleulysgln

100105110

asnleuglualaserglyvalargvalglygluserleuaspargthr

115120125

thrilealaaspileglulysglyleugluaspphetyrtyrserval

130135140

glylystyrseralaservallysalavalvalthrproleuproarg

145150155160

asnargvalaspleulysleuvalpheglngluglyvalseralaglu

165170175

ileglnglnileasnilevalglyasnhisalaphethrthraspglu

180185190

leuileserhispheglnleuargaspgluvalprotrptrpasnval

195200205

valglyasparglystyrglnlysglnlysleualaglyaspleuglu

210215220

thrleuargsertyrtyrleuaspargglytyralaargpheasnile

225230235240

aspserthrglnvalserleuthrproasplyslysglyiletyrval

245250255

thrvalasnilethrgluglyaspglntyrlysleuserglyvalglu

260265270

valserglyasnleualaglyhisseralagluilegluglnleuthr

275280285

lysilegluproglygluleutyrasnglythrlysvalthrlysmet

290295300

gluaspaspilelyslysleuleuglyargtyrglytyralatyrpro

305310315320

argvalglnsermetprogluileasnaspalaasplysthrvallys

325330335

leuargvalasnvalaspalaglyasnargphetyrvalarglysile

340345350

argphegluglyasnaspthrserlysaspalavalleuargargglu

355360365

metargglnmetgluglyalatrpleuglyseraspleuvalaspgln

370375380

glylysgluargleuasnargleuglyphephegluthrvalaspthr

385390395400

aspthrglnargvalproglyserproaspglnvalaspvalvaltyr

405410415

lysvallysgluargasnthrglyserpheasnpheglyileglytyr

420425430

glythrgluserglyvalserpheglnalaglyvalglnglnaspasn

435440445

trpleuglythrglytyralavalglyileasnglythrlysasnasp

450455460

tyrglnthrtyralagluleuservalthrasnprotyrphethrval

465470475480

aspglyvalserleuglyglyargleuphetyrasnasppheglnala

485490495

aspaspalaaspleuserasptyrthrasnlyssertyrglythrasp

500505510

valthrleuglypheproileasnglutyrasnserleuargalagly

515520525

leuglytyrvalhisasnserleuserasnmetglnproglnvalala

530535540

mettrpargtyrleutyrsermetglygluhisproserthrserasp

545550555560

glnaspasnserphelysthraspaspphethrpheasntyrglytrp

565570575

thrtyrasnlysleuaspargglytyrpheprothraspglyserarg

580585590

valasnleuthrglylysvalthrileproglyseraspasnglutyr

595600605

tyrlysvalthrleuaspthralathrtyrvalproileaspaspasp

610615620

hislystrpvalvalleuglyargthrargtrpglytyrglyaspgly

625630635640

leuglyglylysglumetprophetyrgluasnphetyralaglygly

645650655

serserthrvalargglypheglnserasnthrileglyprolysala

660665670

valtyrpheprohisglnalaserasntyraspproasptyrasptyr

675680685

glucysalathrglnaspglyalalysaspleucyslysseraspasp

690695700

alavalglyglyasnalametalavalalaserleuglupheilethr

705710715720

prothrpropheileserasplystyralaasnservalargthrser

725730735

phephetrpaspmetglythrvaltrpaspthrasntrpaspserser

740745750

glntyrserglytyrproasptyrseraspproserasnileargmet

755760765

seralaglyilealaleuglntrpmetserproleuglyproleuval

770775780

phesertyralaglnprophelyslystyraspglyasplysalaglu

785790795800

glnpheglnpheasnileglylysthrtrp

805810

<210>16

<211>376

<212>prt

<213>鲍氏志贺氏菌(shigellaboydii)

<400>16

metlysvallysvalleuserleuleuvalproalaleuleuvalala

151015

glyalaalaasnalaalagluvaltyrasnlysaspglyasnlysleu

202530

aspleutyrglylysvalaspglyleuhistyrpheseraspasnlys

354045

sergluaspglyaspglnthrtyrvalargleuglyphelysglyglu

505560

thrglnvalthraspglnleuthrglytyrglyglntrpglutyrgln

65707580

ileglnglyasnthrsergluaspasnlysgluasnsertrpthrarg

859095

valalaphealaglyleulyspheglnaspvalglyserpheasptyr

100105110

glyargasntyrglyvalvaltyraspvalthrsertrpthraspval

115120125

leuproglupheglyglyaspthrtyrglyseraspasnphemetgln

130135140

glnargglyasnglyphealathrtyrargasnthraspphephegly

145150155160

leuvalaspglyleuasnphealavalglntyrglnglylysasngly

165170175

servalserglygluglymetthrasnasnglyargglyalaleuarg

180185190

glnasnglyaspglyvalglyglyserilethrtyrasptyrglugly

195200205

pheglyileglyalaalavalserserserlysargthraspaspgln

210215220

asnglysertyrileserasnglyvalvalargasntyrileglythr

225230235240

glyaspargalagluthrtyrthrglyglyleulystyraspalaasn

245250255

asniletyrleualaalaglntyrthrglnthrtyrasnalathrarg

260265270

valglyserleuglytrpalaasnlysalaglnasnpheglualaval

275280285

alaglntyrglnpheasppheglyleuargproservalalatyrleu

290295300

glnserlysglylysasnleuglyvalileasnglyargasntyrasp

305310315320

aspgluaspileleulystyrvalaspvalglyalathrtyrtyrphe

325330335

asnlysasnmetserthrtyrvalasptyrlysileasnleuleuasp

340345350

aspasnglnphethrargaspalaglyileasnthraspasnileval

355360365

alaleuglyleuvaltyrglnphe

370375

<210>17

<211>78

<212>prt

<213>福氏志贺氏菌

<400>17

metlysalathrlysleuvalleuglyalavalileleuglyserthr

151015

leuleualaglycysserserasnalalysileaspglnleuserser

202530

aspvalglnthrleuasnalalysvalaspglnleuserasnaspval

354045

asnalametargseraspvalglnalaalalysaspaspalaalaarg

505560

alaasnglnargleuaspasnmetalathrlystyrarglys

657075

<210>18

<211>173

<212>prt

<213>福氏志贺氏菌

<400>18

metglnleuasnlysvalleulysglyleumetilealaleuproval

151015

metalailealaalacysserserasnlysasnalaserasnaspgly

202530

sergluglymetleuglyalaglythrglymetaspalaasnglygly

354045

asnglyasnmetserserglugluglnalaargleuglnmetglngln

505560

leuglnglnasnasnilevaltyrpheaspleuasplystyraspile

65707580

argseraspphealaglnmetleuaspalahisalaasnpheleuarg

859095

serasnprosertyrlysvalthrvalgluglyhisalaaspgluarg

100105110

glythrproglutyrasnileserleuglygluargargalaasnala

115120125

vallysmettyrleuglnglylysglyvalseralaaspglnileser

130135140

ilevalsertyrglylysglulysproalavalleuglyhisaspglu

145150155160

alaalatyrserlysasnargargalavalleuvaltyr

165170

<210>19

<211>48

<212>prt

<213>福氏志贺氏菌

<400>19

metvallyslysthrilealaalailepheservalleuvalleuser

151015

thrvalleuthralacysasnthrthrargglyvalglygluaspile

202530

seraspglyglyasnalaileserglyalaalathrlysalaglngln

354045

<210>20

<211>137

<212>prt

<213>福氏志贺氏菌

<400>20

metlyslysleualailealaglyalaleumetleuleualaglycys

151015

alagluvalgluasntyrasnasnvalvallysthrproalaproasp

202530

trpleualaglytyrtrpglnthrlysglyproglnargalaleuval

354045

serproglualaileglyserleuilevalthrlysgluglyaspthr

505560

leuaspcysargglntrpglnargglyilealavalproglylysleu

65707580

thrleumetseraspaspleuthrasnvalthrvallysarggluleu

859095

tyrgluvalgluargaspglyasnthrileglutyraspglymetthr

100105110

metgluargvalaspargprothralaglucysalaalaalaleuasp

115120125

lysalaproleuprothrproleupro

130135

<210>21

<211>130

<212>prt

<213>福氏志贺氏菌

<400>21

metlyslysphealaalavalilealavalmetalaleucysserala

151015

provalmetalaalagluglnglyglypheserglyproservalthr

202530

glnserglnalaglyglypheglnglyproasnglyservalthrthr

354045

valgluseralalysserleuargaspaspthrtrpvalthrleuarg

505560

glyasnilevalgluargileseraspaspleutyrvalphelysasp

65707580

alaserglythrileasnvalaspileasphislysargtrpasngly

859095

valthrvalthrprolysaspthrvalgluileglnglygluvalasp

100105110

lysasptrpasnservalgluileaspvallysglnilearglysval

115120125

asnpro

130

<210>22

<211>128

<212>prt

<213>福氏志贺氏菌

<400>22

metalaserserserleuilemetglyasnasnmethisvallystyr

151015

leualaglyilevalglyalaalaleuleumetalaglycysserser

202530

serasngluleuseralaalaglyglnservalargilevalaspglu

354045

glnproglyalaglucysglnleuileglythralathrglylysgln

505560

serasntrpleuserglyglnhisglyglugluglyglysermetarg

65707580

glyalaalaasnaspleuargasnglnalaalaalametglyglyasn

859095

valiletyrglyileserserproserglnglymetleuserserphe

100105110

valprothraspserglnileileglyglnvaltyrlyscysproasn

115120125

<210>23

<211>190

<212>prt

<213>福氏志贺氏菌

<400>23

metlysleuvalhismetalaserglyleualavalalailealaleu

151015

alaalacysalaasplysseralaaspileglnthrproalaproala

202530

alaasnthrserileseralathrglnglnproalaileglnglnpro

354045

asnvalserglythrvaltrpileargglnlysvalalaleupropro

505560

aspalavalleuthrvalthrleuseraspalaserleualaaspala

65707580

proserlysvalleualaglnlysalavalargthrgluglylysgln

859095

serpropheserphevalleupropheasnproalaaspvalglnpro

100105110

asnalaargileleuleuseralaalailethrvalasnasplysleu

115120125

valpheilethraspthrvalglnprovalileasnglnglyglythr

130135140

lysalaaspleuthrleuvalprovalglnglnthralavalproval

145150155160

glnalaserglyglyalathrthrthrvalproserthrserprothr

165170175

glnvalasnproserseralavalproalaprothrglntyr

180185190

<210>24

<211>191

<212>prt

<213>福氏志贺氏菌

<400>24

metlysserleuargleumetleucysalametproleumetleuthr

151015

glycysserthrmetserservalasntrpseralaalaasnprotrp

202530

asntrppheglyserserthrlysvalsergluglnglyvalglyglu

354045

leuthralaserthrproleuglngluglnalailealaaspalaleu

505560

aspglyasptyrargleuargserglymetlysthrthrasnglyasn

65707580

valvalargphephegluvalmetlysglyaspasnvalalametval

859095

ileasnglyaspglnglythrileserargileaspvalleuaspser

100105110

aspileproalaaspthrglyvallysileglythrpropheserasp

115120125

leutyrserlysalapheglyasncysglnlysalaaspglyaspasp

130135140

asnargalavalglucyslysalagluglyserglnhisilesertyr

145150155160

glnpheserglyglutrpargglyprogluglyleumetproserasp

165170175

aspthrleulysasntrplysvalserlysileiletrpargarg

180185190

<210>25

<211>76

<212>prt

<213>宋内志贺氏菌

<400>25

metmetlyslysthralaalaileileseralacysmetleuthrphe

151015

alaleuseralacysserglyserasntyrvalmethisthrasnasp

202530

glyargthrilevalseraspglylysproglnthraspasnaspthr

354045

glymetilesertyrlysaspalaasnglyasnlysglnglnileasn

505560

argthraspvallysglumetvalgluleuaspgln

657075

<210>26

<211>344

<212>prt

<213>福氏志贺氏菌

<400>26

metalatyrservalglnlysserargleualalysvalalaglyval

151015

serleuvalleuleuleualaalacysserseraspserargtyrlys

202530

argglnvalserglyaspglualatyrleuglualaalaproleuala

354045

gluleuhisalaproalaglymetileleuprovalthrserglyasp

505560

tyralaileprovalthrasnglyserglyalavalglylysalaleu

65707580

aspileargproproalaglnproleualaleuvalserglyalaarg

859095

thrglnphethrglyaspthralaserleuleuvalgluasnglyarg

100105110

glyasnthrleutrpproglnvalvalservalleuglnalalysasn

115120125

tyrthrilethrglnargaspaspalaglyglnthrleuthrthrasp

130135140

trpvalglntrpasnargleuaspgluaspgluglntyrargglyarg

145150155160

tyrglnileservallysproglnglytyrglnglnalavalthrval

165170175

lysleuleuasnleugluglnalaglylysprovalalaaspalaala

180185190

sermetglnargtyrserthrglumetmetasnvalileseralagly

195200205

leuasplysseralathraspalaalaasnalaalaglnasnargala

210215220

serthrthrmetaspvalglnseralaalaaspaspthrglyleupro

225230235240

metleuvalvalargglypropheasnvalvaltrpglnargleupro

245250255

alaalaleuglulysvalglymetlysvalthraspserthrargser

260265270

glnglyasnmetalavalthrtyrlysproleuseraspserasptrp

275280285

glngluleuglyalaseraspproglyleualaserglyasptyrlys

290295300

leuglnvalglyaspleuaspasnargserserleuglnpheileasp

305310315320

prolysglyhisthrleuthrglnserglnasnaspalaleuvalala

325330335

valpheglnalaalapheserlys

340

<210>27

<211>139

<212>prt

<213>宋内志贺氏菌

<400>27

metargargthrglyasnlysleucysleuilealametilethrala

151015

thrvalalaleuthralacysthrprolysglyservalgluglnhis

202530

thrarghistyrvaltyralaseraspaspglypheaspproasnphe

354045

serthrglnlysalaaspthrthrargmetmetvalprophephearg

505560

glnphetrpaspmetglyalalysasplysalathrglylysserarg

65707580

seraspvalglnglnargileglnglnphehisserglnglupheleu

859095

asnserleuargglythrthrglnphealaglythrasptyrargser

100105110

lysaspleuthrprolyslysserargleuleualaaspthrileser

115120125

alavaltyrleuaspglytyrgluglyarggln

130135

<210>28

<211>193

<212>prt

<213>痢疾志贺氏菌(shigelladysenteriae)

<400>28

metargtyrleualathrleuleuleuserleualavalleuilethr

151015

alaglycysglytrphisleuargaspthrthrglnvalproserthr

202530

metlysvalmetileleuaspserglyaspproasnglyproleuser

354045

argalavalargasnglnleuargleuasnglyvalgluleuleuasp

505560

lysgluthrthrarglysaspvalproserleuargleuglylysval

65707580

serilealalysaspthralaservalpheargasnglyglnthrala

859095

glutyrglnmetilemetthrvalasnalathrvalleuileprogly

100105110

seraspiletyrproileseralalysvalpheargserphepheasp

115120125

asnproglnmetalaleualalysaspasngluglnaspmetileval

130135140

lysglumettyraspargalaalagluglnleuilearglysleupro

145150155160

serileargalaalaaspileargseraspglugluglnthrserthr

165170175

thrthraspthrproalathrproalaargvalserthrthrleugly

180185190

asn

<210>29

<211>427

<212>prt

<213>宋内志贺氏菌

<400>29

metasnthrpheservalserargleualaleualaleualaphegly

151015

valthrleuthralacysserserthrproproaspglnargproser

202530

aspglnthralaproglythrserserargproileleuseralalys

354045

glualaglnasnpheaspalaglnhistyrphealaserleuthrpro

505560

glyalaalaalatrpasnproserproilethrleuproalaglnpro

65707580

aspphevalvalglyproalaglythrglnglyvalthrhisthrthr

859095

ileglnalaalavalaspalaalaileilelysargthrasnlysarg

100105110

glntyrilealavalmetproglyglutyrglnglythrvaltyrval

115120125

proalaalaproglyglyilethrleutyrglythrglyglulyspro

130135140

ileaspvallysileglyleuserleuaspglyglymetserproala

145150155160

asptrparghisaspvalasnproargglylystyrmetproglylys

165170175

proalatrptyrmettyraspsercysglnserlysargseraspser

180185190

ileglyvalleucysseralavalphetrpserglnasnasnglyleu

195200205

glnleuglnasnleuthrilegluasnthrleuglyaspservalasp

210215220

alaglyasnhisproalavalalaleuargthraspglyaspglnval

225230235240

glnileasnasnvalasnileleuglyargglnasnthrphepheval

245250255

thrasnserglyvalglnasnargleugluthrasnargglnproarg

260265270

thrleuvalthrasnsertyrilegluglyaspvalaspilevalser

275280285

glyargglyalavalvalpheaspasnthrglupheargvalvalasn

290295300

serargthrglnglnglualatyrvalphealaproalathrleuser

305310315320

asnmettyrtyrglypheleualavalasnserargpheasnalaphe

325330335

glyaspglyvalalaglnleuglyargserleuaspvalaspalaasn

340345350

thrasnglyglnvalvalileargaspseralaileasngluglyphe

355360365

asnthralalysprotrpalaaspalavalileserasnargprophe

370375380

alaglyasnthrglyservalaspaspasnaspgluileglnargasn

385390395400

leuasnaspthrasntyrasnargmettrpglutyrasnasnarggly

405410415

valglyserlysvalvalalaglualalyslys

420425

<210>30

<211>270

<212>prt

<213>福氏志贺氏菌

<400>30

metlysserleuphelysvalthrleuleualathrthrmetalaval

151015

alaleuhisalaproilethrphealaalaglualaalalysproala

202530

thrthralaaspserlysalaserphelysasnaspaspglnlysser

354045

alatyralaleuglyalaserleuglyargtyrmetgluasnserleu

505560

lysgluglnglulysleuglyilelysleuasplysaspglnleuile

65707580

alaglyvalglnaspalaphealaasplysserlysleuseraspgln

859095

gluilegluglnthrleuglnalapheglualaargvallysserser

100105110

alaglnalalysmetglulysaspalaalaaspasnglualalysgly

115120125

lysglutyrargglulysphealalysglulysglyvallysthrser

130135140

serthrglyleuvaltyrglnvalvalglualaglylysglygluala

145150155160

prolysaspseraspthrvalvalvalasntyrlysglythrleuile

165170175

aspglylysglupheaspasnsertyrthrargglygluproleuser

180185190

pheargleuaspglyvalileproglytrpthrgluglyleulysasn

195200205

ilelyslysglyglylysilelysleuvalileproprogluleuala

210215220

tyrglylysalaglyvalproglyileproproasnserthrleuval

225230235240

pheaspvalgluleuleuaspvallysproalaprolysalaaspala

245250255

lysproglualaaspalalysalaalaaspseralalyslys

260265270

<210>31

<211>260

<212>prt

<213>宋内志贺氏菌

<400>31

metlyslysleuvalleuserleuserleuvalleualapheserser

151015

alathralaalaphealaalaileproglnasnileargileglythr

202530

aspprothrtyralaprophegluserlysasnserglnglygluleu

354045

valglypheaspileaspleualalysgluleucyslysargileasn

505560

thrglncysthrphevalgluasnproleuaspalaleuileproser

65707580

leulysalalyslysileaspalailemetserserleuserilethr

859095

glulysargglnglngluilealaphethrasplysleutyralaala

100105110

aspserargleuvalvalalalysasnseraspileglnprothrval

115120125

gluserleulysglylysargvalglyvalleuglnglythrthrgln

130135140

gluthrpheglyasngluhistrpalaprolysglyilegluileval

145150155160

sertyrglnglyglnaspasniletyrseraspleuthralaglyarg

165170175

ileaspalaalapheglnaspgluvalalaalasergluglypheleu

180185190

lysglnprovalglylysasptyrlyspheglyglyproservallys

195200205

aspglulysleupheglyvalglythrglymetglyleuarglysglu

210215220

aspasngluleuargglualaleuasnlysalaphealaglumetarg

225230235240

alaaspglythrtyrglulysleualalyslystyrpheasppheasp

245250255

valtyrglygly

260

<210>32

<211>474

<212>prt

<213>福氏志贺氏菌

<400>32

metlyslysasnthrleualaleuseralaleualaleuserleugly

151015

leualaleuserproleuseralathralaalagluthrserserala

202530

thrthralaglnglnmetproserleualaprometleuglulysval

354045

metproservalvalserileasnvalgluglyserthrthrvalasn

505560

thrproargmetproargasnpheglnglnphepheglyaspaspser

65707580

prophecysglngluglyserpropheglnserserprophecysgln

859095

glyglyglnglyglyasnglyglyglyglnglnglnlysphemetala

100105110

leuglyserglyvalileileaspalaasplysglytyrvalvalthr

115120125

asnasnhisvalvalaspasnalathrvalilelysvalglnleuser

130135140

aspglyarglyspheaspalalysmetvalglylysaspproargser

145150155160

aspilealaleuileglnileglnasnprolyslysleuthralaile

165170175

lysmetalaaspseraspalaleuargvalglyasptyrthrvalala

180185190

ileglyasnpropheglyleuglygluthrvalthrserglyileval

195200205

seralaleuglyargserglyleuasnalagluasntyrgluasnphe

210215220

ileglnthraspalaalaileasnargglyasnserglyglyalaleu

225230235240

valasnleuasnglygluleuileglyileasnthralaileleuala

245250255

proaspglyglyasnileglyileglyphealaileproserasnmet

260265270

vallysasnleuthrserglnmetvalglutyrglyglnvallysarg

275280285

glygluleuglyilemetglythrgluleuasnsergluleualalys

290295300

alametlysvalaspalaglnargglyalaphevalserglnvalleu

305310315320

proasnserseralaalalysalaglyilelysalaglyaspvalile

325330335

thrserleuasnglylysproileserserphealaalaleuargala

340345350

glnvalglythrmetprovalglyserlysleuthrleuglyleuleu

355360365

argaspglylysglnvalasnvalasnleugluleuglnglnserser

370375380

glnasnglnvalaspserserserilepheasnglyilegluglyala

385390395400

glumetserasnlysglylysaspglnglyvalvalvalasnasnval

405410415

lysthrglythrproalaalaglnileglyleulyslysglyaspval

420425430

ileileglyalaasnglnglnalavallysasnilealagluleuarg

435440445

lysvalleuaspserlysproservalleualaleuasnileglnarg

450455460

glyaspserthriletyrleuleumetgln

465470

<210>33

<211>430

<212>prt

<213>福氏志贺氏菌

<400>33

metlysglnalaleuargvalalapheglypheleuileleutrpala

151015

servalleuhisalagluvalargilevalileaspserglyvalasp

202530

serglyargproileglyvalvalpropheglntrpalaglyprogly

354045

alaalaprogluaspileglyglyilevalalaalaaspleuargasn

505560

serglylyspheasnproleuaspargalaargleuproglnglnpro

65707580

glyseralaglngluvalglnproalaalatrpseralaleuglyile

859095

aspalavalvalvalglyglnvalthrproasnproaspglysertyr

100105110

asnvalalatyrglnleuvalaspthrglyglyalaproglythrval

115120125

leualaglnasnsertyrlysvalasnlysglntrpleuargtyrala

130135140

glyhisthralaseraspgluvalpheglulysleuthrglyilelys

145150155160

glyalapheargthrargilealatyrvalvalglnthrasnglygly

165170175

glnpheprotyrgluleuargvalserasptyraspglytyrasngln

180185190

phevalvalhisargserproglnproleumetserproalatrpser

195200205

proaspglyserlysleualatyrvalthrphegluserglyargser

210215220

alaleuvalileglnthrleualaasnglyalavalargglnvalala

225230235240

serpheproarghisasnglyalaproalapheserproaspglyser

245250255

lysleualaphealaleuserlysthrglyserleuasnleutyrval

260265270

metaspleualaserglyglnileargglnvalthraspglyargser

275280285

asnasnthrgluprothrtrppheproaspserglnasnleualaphe

290295300

thrseraspglnalaglyargproglnvaltyrlysvalasnileasn

305310315320

glyglyalaproglnargilethrtrpgluglyserglnasnglnasp

325330335

alaaspvalserseraspglylysphemetvalmetvalserserasn

340345350

glyglyglnglnhisilealalysglnaspleualathrglyglyval

355360365

glnvalleuserserthrpheleuaspgluthrproserleualapro

370375380

asnglythrmetvaliletyrserserserglnglymetglyserval

385390395400

leuasnleuvalserthraspglyargphelysalaargleuproala

405410415

thraspglyglnvallyspheproalatrpserprotyrleu

420425430

<210>34

<211>257

<212>prt

<213>福氏志贺氏菌

<400>34

metalaarglystrpleuasnleuphealaglyalaalaleuserphe

151015

alavalalaglyasnalaleualaaspgluglylysilethrvalphe

202530

alaalaalaserleuthrasnalametglnaspilealathrglntyr

354045

lyslysglulysglyvalaspvalvalserserphealaserserser

505560

thrleualaargglnileglualaglyalaproalaaspleupheile

65707580

seralaaspglnlystrpmetasptyralavalasplyslysalaile

859095

aspthralathrargglnthrleuleuglyasnserleuvalvalval

100105110

alaprolysalasergluglnlysaspphethrileaspserlysthr

115120125

asntrpthrserleuleuasnglyglyargleualavalglyasppro

130135140

gluhisvalproalaglyiletyralalysglualaleuglnlysleu

145150155160

glyalatrpaspthrleuserprolysleualaproalagluaspval

165170175

argglyalaleualaleuvalgluargasnglualaproleuglyile

180185190

valtyrglyseraspalavalalaserlysglyvallysvalvalala

195200205

thrpheprogluaspserhislyslysvalglutyrprovalalaval

210215220

valgluglyhisasnasnalathrvallysalaphetyrasptyrleu

225230235240

lysglyproglnalaalagluilephelysargtyrglyphethrthr

245250255

lys

<210>35

<211>193

<212>prt

<213>福氏志贺氏菌

<400>35

metphelysserthrleualaalametalaalavalphealaleuser

151015

alaleuseralaleuserproalaalametalaalalysglyasppro

202530

hisvalleuleuthrthrseralaglyasnilegluleugluleuasp

354045

lysglnlysalaprovalservalglnasnphevalasptyrvalasn

505560

serglyphetyrasnasnthrthrphehisargvalileproglyphe

65707580

metileglnglyglyglyphethrgluglnmetglnglnlyslyspro

859095

asnproproilelysasnglualaaspasnglyleuargasnthrarg

100105110

glythrilealametalaargthralaasplysaspseralathrser

115120125

glnphepheileasnvalalaaspasnalapheleuasphisglygln

130135140

argasppheglytyralavalpheglylysvalvallysglymetasp

145150155160

valalaasplysileserglnvalprothrhisaspvalglyprotyr

165170175

glnasnvalproserlysprovalvalileleuseralalysvalleu

180185190

pro

<210>36

<211>428

<212>prt

<213>福氏志贺氏菌

<400>36

metlysasntrplysthrleuleuleuglyilealametilealaasn

151015

thrserphealaalaproglnvalvalasplysvalalaalavalval

202530

asnasnglyvalvalleugluseraspvalaspglyleumetglnser

354045

vallysleuasnalaalaglnalaargglnglnleuproaspaspala

505560

thrleuarghisglnilemetgluargleuilemetaspglnileile

65707580

leuglnmetglyglnlysmetglyvallysileseraspgluglnleu

859095

aspglnalailealaasnilealalysglnasnasnmetthrleuasp

100105110

glnmetargserargleualatyraspglyleuasntyrasnthrtyr

115120125

argasnglnilearglysglumetileilesergluvalargasnasn

130135140

gluvalargargargilethrileleuproglngluvalgluserleu

145150155160

alaglnglnvalglyasnglnasnaspalaserthrgluleuasnleu

165170175

serhisileleuileproleuprogluasnprothrseraspglnval

180185190

asnglualagluserglnalaargalailevalaspglnalaargasn

195200205

glyalaasppheglylysleualailealahisseralaaspglngln

210215220

alaleuasnglyglyglnmetglytrpglyargileglngluleupro

225230235240

glyilephealaglnalaleuserthralalyslysglyaspileval

245250255

glyproileargserglyvalglyphehisileleulysvalasnasp

260265270

leuargglygluserlysasnileservalthrgluvalhisalaarg

275280285

hisileleuleulysproserproilemetthraspgluglnalaarg

290295300

vallysleugluglnilealaalaaspilelysserglylysthrthr

305310315320

phealaalaalaalalysglupheserglnaspproglyseralaasn

325330335

glnglyglyaspleuglytrpalathrproaspilepheaspproala

340345350

pheargaspalaleuthrargleuasnlysglyglnmetseralapro

355360365

valhisserserpheglytrphisleuilegluleuleuaspthrarg

370375380

asnvalasplysthraspalaalaglnlysaspargalatyrargmet

385390395400

leumetasnarglysphesergluglualaalasertrpmetglnglu

405410415

glnargalaseralatyrvallysileleuserasn

420425

<210>37

<211>543

<212>prt

<213>福氏志贺氏菌

<400>37

metthrasnilethrlysargserleuvalalaalaglyvalleuala

151015

alaleumetalaglyasnvalalaleualaalaaspvalproalagly

202530

valthrleualaglulysglnthrleuvalargasnasnglyserglu

354045

valglnserleuaspprohislysilegluglyvalprogluserasn

505560

ileserargaspleuphegluglyleuleuvalseraspleuaspgly

65707580

hisproalaproglyvalalaglusertrpaspasnlysaspalalys

859095

valtrpthrphehisleuarglysaspalalystrpseraspglythr

100105110

provalthralaglnaspphevaltyrsertrpglnargservalasp

115120125

proasnthralaserprotyralasertyrleuglntyrglyhisile

130135140

alaglyileaspgluileleugluglylyslysproilethraspleu

145150155160

glyvallysalaileaspasphisthrleugluvalthrleuserglu

165170175

provalprotyrphetyrlysleuleuvalhisproserthrserpro

180185190

valprolysalaalaileglulyspheglyglulystrpthrglnpro

195200205

glyasnilevalthrasnglyalatyrthrleulysasptrpvalval

210215220

asngluargilevalleugluargserprothrtyrtrpasnasnala

225230235240

lysthrvalileasnglnvalthrtyrleuproilealasergluval

245250255

thraspvalasnargtyrargserglygluileaspmetthrtyrasn

260265270

asnmetproilegluleupheglnlysleulyslysgluileproasp

275280285

gluvalhisvalaspprotyrleucysthrtyrtyrtyrgluileasn

290295300

asnglnlyspropropheasnaspvalargvalargthralaleulys

305310315320

leuglymetaspargaspileilevalasnlysvallysalaglngly

325330335

aspmetproalatyrglytyrthrproprotyrthraspglyalalys

340345350

leuthrglnproglutrppheglytrpserglnglulysargasnglu

355360365

glualalyslysleuleualaglualaglytyrthralaasplyspro

370375380

leuthrileasnleuargtyrasnthrseraspleuhislyslysleu

385390395400

alailealaalaserserleutrplyslysasnileglyvalasnval

405410415

lysleuvalasnglnglutrplysthrpheleuaspthrarghisgln

420425430

glythrpheaspvalalaargalaglytrpcysalaasptyrasnglu

435440445

prothrserpheleuasnthrmetleuserasnsersermetasnthr

450455460

alahistyrlysserproalapheaspserilemetalagluthrleu

465470475480

lysvalthraspglualaglnargthralaleutyrthrlysalaglu

485490495

glnglnleuasplysaspseralailevalprovaltyrtyrtyrval

500505510

asnalaargleuvallysprotrpvalglyglytyrthrglylysasp

515520525

proleuaspasnthrtyrthrargasnmettyrilevallyshis

530535540

<210>38

<211>201

<212>prt

<213>福氏志贺氏菌

<400>38

metthrmetthrargleulysileserlysthrleuleualavalmet

151015

leuthrseralavalalathrglyseralatyralagluasnasnala

202530

glnthrthrasngluseralaglyglnlysvalaspsersermetasn

354045

lysvalglyasnphemetaspaspseralailethralalysvallys

505560

alaalaleuvalasphisaspasnilelysserthraspileserval

65707580

lysthraspglnlysvalvalthrleuserglyphevalglusergln

859095

alaglnalagluglualavallysvalalalysglyvalgluglyval

100105110

thrservalserasplysleuhisvalargaspalalysgluglyser

115120125

vallysglytyralaglyaspthralathrthrsergluilelysala

130135140

lysleuleualaaspaspilevalproserarglysvallysvalglu

145150155160

thrthraspglyvalvalglnleuserglythrvalaspserglnala

165170175

glnseraspargalagluserilealalysalavalaspglyvallys

180185190

servallysasnaspleulysthrlys

195200

<210>39

<211>243

<212>prt

<213>宋内志贺氏菌

<400>39

metlyslysleuvalleualaalaleuleualaserphethrphegly

151015

alaseralaalaglulysileasnpheglyvalseralathrtyrpro

202530

prophegluserileglyalaasnasngluilevalglypheaspile

354045

aspleualalysalaleucyslysglnmetglnalaglucysthrphe

505560

thrasnhisalapheaspserleuileproserleulysphearglys

65707580

tyraspservalileserglymetaspilethrprogluargserlys

859095

glnvalserphethrthrprotyrtyrgluasnseralavalvalile

100105110

alalyslysaspthrtyrlysthrphealaaspleulysglylysarg

115120125

ileglymetgluasnglythrthrhisglnlystyrileglnaspgln

130135140

hisprogluvallysthrvalsertyraspsertyrglnasnalaphe

145150155160

ileaspleulysasnglyargileaspglyvalpheglyaspthrala

165170175

valvalasnglutrpleulysthrasnproglnleuglyvalalathr

180185190

glulysvalthraspproglntyrpheglythrglyleuglyileala

195200205

valargproaspasnlysalaleuleuglulysleuasnasnalaleu

210215220

alaalailelysalaaspglythrtyrglnlysileasnaspglntrp

225230235240

pheprogln

<210>40

<211>550

<212>prt

<213>宋内志贺氏菌

<400>40

metlysleuleuglnargglyvalalaleualaleuleuthrthrphe

151015

thrleualasergluthralaleualatyrgluglnasplysthrtyr

202530

lysilethrvalleuhisthrasnasphishisglyhisphetrparg

354045

asnglutyrglyglutyrglyleualaalaglnlysthrleuvalasp

505560

glyilearglysgluvalalaalagluglyglyservalleuleuleu

65707580

serglyglyaspileasnthrglyvalprogluseraspleuglnasp

859095

alagluproasppheargglymetasnleuvalglytyraspalamet

100105110

alaileglyasnhisglupheaspasnproleuthrvalleuarggln

115120125

glnglulystrpalalyspheproleuleuseralaasniletyrgln

130135140

lysserthrglygluargleuphelysprotrpalaleuphelysarg

145150155160

glnaspleulysilealavalileglyleuthrthraspaspthrala

165170175

lysileglyasnproglutyrphethraspilegluphearglyspro

180185190

alaaspglualalysleuvalileglngluleuglnglnthrglulys

195200205

proaspileileilealaalathrhismetglyhistyraspasngly

210215220

gluhisglyserasnalaproglyaspvalglumetalaargalaleu

225230235240

proalaglyserleualametilevalglyglyhisserglnasppro

245250255

valcysmetalaalagluasnlyslysglnvalasptyrvalprogly

260265270

thrprocyslysproaspglnglnasnglyiletrpilevalglnala

275280285

hisglutrpglylystyrvalglyargalaasppheglupheargasn

290295300

glyglumetlysmetvalasntyrglnleuileprovalasnleulys

305310315320

lyslysvalthrarggluaspglylyssergluargvalleutyrthr

325330335

progluilealagluasnglnglnmetileserleuleuserprophe

340345350

glnasnlysglylysalaglnleugluvallysileglygluthrasn

355360365

glyargleugluglyaspargasplysvalargphevalglnthrasn

370375380

metglyargleuileleualaalaglnmetaspargthrglyalaasp

385390395400

phealavalmetserglyglyglyileargaspserileglualagly

405410415

aspilesertyrlysasnvalleulysvalglnpropheglyasnval

420425430

valvaltyralaaspmetileglylysgluvalileasptyrleuthr

435440445

alavalalaglnmetlysproaspserglyalatyrproglnpheala

450455460

asnvalserphevalalalysaspglylysleuasnaspleulysile

465470475480

lysglygluprovalaspproalalysthrtyrargmetalathrleu

485490495

asnpheasnalathrglyglyaspglytyrproargleuaspasnlys

500505510

proglytyrvalasnthrglypheileaspalagluvalleulysala

515520525

tyrileglnlysserserproleuaspvalservaltyrgluprolys

530535540

glygluvalsertrpgln

545550

<210>41

<211>266

<212>prt

<213>宋内志贺氏菌

<400>41

metlysleualahisleuglyargglnalaleumetglyvalmetala

151015

valalaleuvalalaglymetservallysserphealaaspglugly

202530

leuleuasnlysvallysgluargglythrleuleuvalglyleuglu

354045

glythrtyrpropropheserpheglnglyaspaspglylysleuthr

505560

glyphegluvalgluphealaglnglnleualalyshisleuglyval

65707580

glualaserleulysprothrlystrpaspglymetleualaserleu

859095

aspserlysargileaspvalvalileasnglnvalthrileserasp

100105110

gluarglyslyslystyrasppheserthrprotyrthrilesergly

115120125

ileglnalaleuvallyslysglyasngluglythrilelysthrala

130135140

aspaspleulysglylyslysvalglyvalglyleuglythrasntyr

145150155160

gluglutrpleuargglnasnvalglnglyvalaspvalargthrtyr

165170175

aspaspaspprothrlystyrglnaspleuargvalglyargileasp

180185190

alaileleuvalaspargleualaalaleuaspleuvallyslysthr

195200205

asnaspthrleualavalthrglyglualapheserargglngluser

210215220

glyvalalaleuarglysglyasngluaspleuleulysalavalasn

225230235240

aspalailealaglumetglnlysaspglythrleuglnalaleuser

245250255

glulystrppheglyalaaspvalthrlys

260265

<210>42

<211>508

<212>prt

<213>福氏志贺氏菌

<400>42

metargtrpleuseralaalavalmetleuthrleutyrthrserser

151015

sertrpalapheserileaspaspvalalalysglnalaglnserleu

202530

alaglylysglytyrglualaprolysserasnleuproservalphe

354045

argaspmetlystyralaasptyrglnglnileglnpheasnhisasp

505560

lysalatyrtrpasnasnleulysthrprophelysleugluphetyr

65707580

hisglnglymettyrpheaspthrprovallysileasngluvalthr

859095

alathralavallysargilelystyrserproasptyrphethrphe

100105110

glyaspvalglnhisasplysaspthrvallysaspleuglypheala

115120125

glyphelysvalleutyrproileasnserlysasplysasnaspglu

130135140

ilevalsermetleuglyalasertyrpheargvalileglyalagly

145150155160

glnvaltyrglyleuseralaargglyleualaileaspthralaleu

165170175

proserglygluglupheproargphelysgluphetrpilegluarg

180185190

prolysprothrasplysargleuthriletyralaleuleuaspser

195200205

proargalathrglyalatyrlysphevalvalmetproglyargasp

210215220

thrvalvalaspvalglnserlysiletyrleuargasplysvalgly

225230235240

lysleuglyvalalaproleuthrsermetpheleupheglyproasn

245250255

glnproserproalaasnasntyrargprogluleuhisaspserasn

260265270

glyleuserilehisalaglyasnglyglutrpiletrpargproleu

275280285

asnasnprolyshisleualavalserserphesermetgluasnpro

290295300

argglypheglyleuleuglnargglyargasppheserargpheglu

305310315320

aspleuaspaspargtyraspleuargproseralatrpvalthrpro

325330335

lysglyglutrpglylysglyservalgluleuvalgluileprothr

340345350

asnaspgluthrasnaspasnilevalalatyrtrpthrproaspgln

355360365

leuprogluproglylysglumetasnphelystyrthrilethrphe

370375380

serargaspgluasplysleuhisalaproaspasnalatrpvalgln

385390395400

glnthrargargserthrglyaspvallysglnserasnleuilearg

405410415

glnproaspglythrilealaphevalvalaspphethrglyalaglu

420425430

metlyslysleuprogluaspthrprovalthralaglnthrserile

435440445

glyaspasnglygluilevalgluserthrvalargtyrasnproval

450455460

thrlysglytrpargleuvalmetargvallysvallysaspalalys

465470475480

lysthrthrglumetargalaalaleuvalasnalaaspglnthrleu

485490495

sergluthrtrpsertyrglnleuproalaasnglu

500505

<210>43

<211>338

<212>prt

<213>宋内志贺氏菌

<400>43

metalavalasnleuleulyslysasnserleualaleuvalalaser

151015

leuleuleualaglyhisvalglnalathrgluleuleuasnserser

202530

tyraspvalserarggluleuphealaalaleuasnpropropheglu

354045

glnglntrpthrlysgluasnglyglyasplysleuthrilelysgln

505560

serhisalaglyserserlysglnalaleualaileleuglnglyleu

65707580

lysalaaspvalvalthrtyrasnglnvalthraspvalglnileleu

859095

hisasplysglylysleuileproalaasptrpglnthrargleupro

100105110

asnasnserserprophetyrserthrmetglypheleuvalarglys

115120125

glyasnprolysasnilehisasptrpasnaspleuvalargserasp

130135140

vallysleuilepheproasnprolysthrserglyasnalaargtyr

145150155160

thrtyrleualaalatrpglyalaalaasplysalaaspglyglyasp

165170175

lysalalysthrgluglnphemetthrglnpheleulysasnvalglu

180185190

valpheaspthrglyglyargglyalathrthrthrphealagluarg

195200205

glyleuglyaspvalleuileserpheglusergluvalasnasnile

210215220

arglysglntyrglualaglnglyphegluvalvalileprolysthr

225230235240

asnileleualaglupheprovalalatrpvalasplysasnvalgln

245250255

alaasnglythrglulysalaalalysalatyrleuasntrpleutyr

260265270

serproglnalaglnthrileilethrasptyrtyrtyrargvalasn

275280285

asnprogluvalmetasplysleulysasplyspheproglnthrglu

290295300

leupheargvalgluasplyspheglysertrpprogluvalmetlys

305310315320

thrhisphethrserglyglygluleuasplysleuleualaalagly

325330335

argasn

<210>44

<211>191

<212>prt

<213>福氏志贺氏菌

<400>44

metlysalaleuserproilealavalleuileseralaleuleuleu

151015

glnglycysvalalaalaalavalvalglythralaalavalglythr

202530

lysalaalathraspproargservalglythrglnvalaspaspgly

354045

thrleugluvalargvalasnseralaleuserlysaspgluglnile

505560

lyslysgluthrargileasnvalthralatyrglnglylysvalleu

65707580

leuvalglyglnserproasnalagluleuseralaargalalysgln

859095

ilealametglyvalaspglyalaasngluvaltyrasngluilearg

100105110

glnglyglnproileglyleuglyglualaserasnaspthrtrpile

115120125

thrthrlysvalargserglnleuleuthrseraspleuvallysser

130135140

serasnvallysvalthrthrgluasnglygluvalpheleumetgly

145150155160

leuvalthrgluargglualalysalaalaalaaspilealaserarg

165170175

valserglyvallysargvalthrthralaphethrpheilelys

180185190

<210>45

<211>353

<212>prt

<213>宋内志贺氏菌

<400>45

methisleuarghisleupheserserargleuargglyserleuleu

151015

leuglyserleuleuvalalaserserpheserthrglnalaalaglu

202530

glumetleuarglysalavalglylysglyalatyrglumetalatyr

354045

serglnglngluasnalaleutrpleualathrserglnserarglys

505560

leuasplysglyglyvalvaltyrargleuaspprovalthrleuglu

65707580

valthrglnalailehisasnaspleulyspropheglyvalthrile

859095

asnasnthrthrglnthrleutrppheglyasnthrvalasnserala

100105110

valthralaileaspalalysthrglygluvallysglyargleuval

115120125

leuaspasparglysargthrglugluvalargproleuglnproarg

130135140

gluleuvalalaaspaspalathrasnthrvaltyrileserglyile

145150155160

glylysgluservaliletrpvalvalaspglygluasnilelysleu

165170175

lysthralaileglnasnthrglylysmetserthrglyleualaleu

180185190

aspserlysglylysargleutyrthrthrasnalaaspglygluleu

195200205

ilethrileaspthralaaspasnlysileleuserarglyslysleu

210215220

leuaspaspglylysgluhisphepheileasnileserleuaspthr

225230235240

alaargglnargalapheilethraspserlysalaalagluvalleu

245250255

valvalaspthrargasnglyasnileleualalysvalalaalapro

260265270

gluserleualavalleupheasnproalaargasnglualatyrval

275280285

thrhisargglnalaglylysvalservalileaspalalyssertyr

290295300

lysvalvallysthrpheaspthrprothrhisproasnserleuala

305310315320

leuseralaaspglylysthrleutyrvalservallysglnlysser

325330335

thrlysglnglnglualathrglnproaspaspvalileargileala

340345350

leu

<210>46

<211>502

<212>prt

<213>宋内志贺氏菌

<400>46

metasnlysserleuvalalavalglyvalilevalalaleuglyval

151015

valtrpthrglyglyalatrptyrthrglylyslysilegluthrhis

202530

leugluaspmetvalalaglnalaasnalaglnleulysleuthrala

354045

progluserasnleugluvalsertyrglnasntyrhisargglyval

505560

pheserserglnleuglnleuleuvallysproilealaglylysglu

65707580

asnprotrpilelysserglyglnservalilepheasngluserval

859095

asphisglypropheproleualaglnleulyslysleuasnleuile

100105110

prosermetalaserileglnthrthrleuvalasnasngluvalser

115120125

lysproleupheaspmetalalysglygluthrprophegluileasn

130135140

serargileglytyrserglyaspserserseraspileserleulys

145150155160

proleuasntyrgluglnlysaspglulysvalalapheserglygly

165170175

glupheglnleuasnalaaspargaspglylysalaileserleuser

180185190

glyglualaglnserglyargileaspalavalasnglutyrasngln

195200205

lysvalglnleuthrpheasnasnleulysthraspglyserserthr

210215220

leualaserpheglygluargvalglyasnglnlysleuserleuglu

225230235240

lysmetthrileservalgluglylysgluleualaleuleuglugly

245250255

metgluileserglylysseraspleuvalasnaspglylysthrile

260265270

asnserglnleuasptyrserleuasnserleulysvalglnasngln

275280285

aspleuglyserglylysleuthrleulysvalglyglnileaspgly

290295300

glualatrphisglnpheserglnglntyrasnalaglnthrglnala

305310315320

leuleualaglnprogluilealaasnasnprogluleutyrglnglu

325330335

lysvalthrglualaphepheseralaleuproleumetleulysgly

340345350

aspprovalilethrilealaproleusertrplysasnserglngly

355360365

gluseralaleuasnleuserleupheleulysaspproalathrthr

370375380

lysglualaproglnthrleualaglngluvalaspargservallys

385390395400

serleuaspalalysleuthrileprovalaspmetalathrgluphe

405410415

metthrglnvalalalysleugluglytyrglngluaspglnalalys

420425430

lysleualalysglnglnvalgluglyalaseralametglyglnmet

435440445

pheargleuthrthrleuglnaspasnthrilethrthrserleugln

450455460

tyrthrasnglyglnilethrleuasnglyglnlysmetproleuglu

465470475480

aspphevalglymetphealametproalaleuasnvalproalaval

485490495

proalaileproglngln

500

<210>47

<211>269

<212>prt

<213>福氏志贺氏菌

<400>47

metglulyslysileglypheileglycysglyasnmetglylysala

151015

ileleuglyglyleuilealaserglyglnvalleuproglyglnile

202530

trpvaltyrthrproserproasplysvalalaalaleuhisaspgln

354045

pheglyileasnalaalagluseralaglngluvalalaglnileala

505560

aspileilephealaalavallysproglyilemetilelysvalleu

65707580

sergluilethrserserleuasnlysaspserleuvalvalserile

859095

alaalaglyvalthrleuaspglnleualaargalaleuglyhisasp

100105110

arglysileileargalametproasnthrproalaleuvalasnala

115120125

glymetthrservalthrproasnalaleuvalthrprogluaspthr

130135140

alaaspvalleuasnilepheargcyspheglyglualagluvalile

145150155160

alagluprometilehisprovalvalglyvalserglyserserpro

165170175

alatyrvalphemetpheileglualametalaaspalaalavalleu

180185190

glyglymetproargalaglnalatyrlysphealaalaglnalaval

195200205

metglyseralalysmetvalleugluthrglygluhisproglyala

210215220

leulysaspmetvalcysserproglyglythrthrileglualaval

225230235240

argvalleugluglulysglypheargalaalavalileglualamet

245250255

thrlyscysmetglulysserglulysleuserlysser

260265

<210>48

<211>307

<212>prt

<213>福氏志贺氏菌

<400>48

metargserargpheseralaphetyrprolysalaasntyrglyleu

151015

glnglyserglnproseraspvalargalahisasnargalaalaasn

202530

glyalacysaspglutyrlysglnleulysvalleusermetglyarg

354045

glnlysalavalilelysalaargargglualalysargvalleuarg

505560

argaspserargserhislysglnarggluglugluservalthrser

65707580

leuvalglnmetserglyvalglualaileglymetalaargaspser

859095

argaspthrserproileleualaargasnglualaglnleuhistyr

100105110

leuglnalailegluserlysglnleuilephealathrglygluala

115120125

glycysglylysthrtrpileseralaalalysalaalaglualaleu

130135140

ilehislysaspvalaspargileilevalthrargprovalleugln

145150155160

alaaspgluaspleuglypheleuproglyaspilealaglulysphe

165170175

alaprotyrpheargprovaltyraspleuleuvalargargleugly

180185190

alaserphemetglntyrcysleuargprogluileglylysvalglu

195200205

thralaprophealatyrmetargglyargthrphegluasnalaval

210215220

valileleuaspglualaglnasnvalthralaalaglnmetlysmet

225230235240

pheleuthrargleuglygluasnvalthrvalilevalasnglyasp

245250255

ilethrglncysaspleuproargglyvalcysserglyleuserasp

260265270

alaleugluargpheglugluaspglumetvalglyilevalargphe

275280285

glylysgluaspcysvalargseralaleucysglnargthrleuhis

290295300

alatyrser

305

<210>49

<211>235

<212>prt

<213>福氏志贺氏菌

<400>49

metglyileserglymetalaalaglnalaasngluleuproaspgly

151015

prohisilevalthrserglythralaservalaspalavalproasp

202530

ilealathrleualailegluvalasnvalalaalalysaspalaala

354045

thralalyslysglnalaaspgluargvalalaglntyrileserphe

505560

leugluleuasnglnilealalyslysaspileserseralaasnleu

65707580

argthrglnproasptyrasptyrglnaspglylysserileleulys

859095

glytyrargalavalargthrvalgluvalthrleuargglnleuasp

100105110

lysleuasnserleuleuaspglyalaleulysalaglyleuasnglu

115120125

ileargservalserleuglyvalalaglnproaspalatyrlysasp

130135140

lysalaarglysalaalaileaspasnalailehisglnalaglnglu

145150155160

leualaasnglyphehisarglysleuglyprovaltyrservalarg

165170175

tyrhisvalserasntyrglnproserprometvalargmetmetlys

180185190

alaaspalaalaprovalseralaglngluthrtyrgluglnalaala

195200205

ileglnpheaspaspglnvalaspvalvalpheglnleugluproval

210215220

aspglnglnproalalysthrproalaalagln

225230235

<210>50

<211>314

<212>prt

<213>宋内志贺氏菌

<400>50

metlysleulysasnthrleuleualaseralaleuleuseralathr

151015

alapheservalasnalaalathrgluleuthrprogluglnalaala

202530

alavallyspropheaspargvalvalvalthrglyargpheasnala

354045

ileglyglualavallysalavalserargargalaasplysglugly

505560

alaalaserphetyrvalvalaspthrserasppheglyasnsergly

65707580

asntrpargvalvalalaaspleutyrlysalaaspalaglulysala

859095

glugluthrserasnargvalileasnglyvalvalgluleuprolys

100105110

aspglnalavalleuileglupropheaspthrvalthrvalglngly

115120125

phetyrargserglnprogluvalasnaspalailethrlysalaala

130135140

lysalalysglyalatyrserphetyrilevalargglnileaspala

145150155160

asnglnglyglyasnglnargilethralapheiletyrlyslysasp

165170175

alalyslysargilevalglnserproaspvalileproalaaspser

180185190

glualaglyargalaalaleualaalaglyglyglualaalalyslys

195200205

valgluileleuglyvalalathrthralaserprosersergluval

210215220

glyargphephegluthrglnserserlysglyglyargtyrthrval

225230235240

metleuproaspglythrlysvalglugluleuasnlysalathrala

245250255

alametmetvalpropheaspserilelyspheserglyasntyrgly

260265270

asnmetthrgluvalsertyrglnvalalalysargalaalalyslys

275280285

glyalalystyrtyrhisilethrargglntrpglngluargglyasn

290295300

asnleuthrvalseralaaspleutyrlys

305310

<210>51

<211>211

<212>prt

<213>宋内志贺氏菌

<400>51

metphelysargleumetmetvalalaleuleuvalilealaproleu

151015

seralaalathralaalaaspglnthrasnprotyrlysleumetasp

202530

glualaalaglnlysthrpheaspargleulysasngluglnprogln

354045

ileargalaasnproasptyrleuargthrilevalaspglngluleu

505560

leuprotyrvalglnvallystyralaglyalaleuvalleuglygln

65707580

tyrtyrlysseralathrproalaglnargglualatyrphealaala

859095

pheargglutyrleulysglnalatyrglyglnalaleualamettyr

100105110

hisglyglnthrtyrglnilealaprogluglnproleuglyasplys

115120125

thrilevalproileargvalthrileileaspproasnglyargpro

130135140

provalargleuasppheglntrparglysasnserglnthrglyasn

145150155160

trpglnalatyraspmetilealagluglyvalsermetilethrthr

165170175

lysglnasnglutrpglythrleuleuargthrlysglyileaspgly

180185190

leuthralaglnleulysserileserglnglnlysilethrleuglu

195200205

glulyslys

210

<210>52

<211>548

<212>prt

<213>福氏志贺氏菌

<400>52

metalaalalysaspvallyspheglyasnaspalaargvallysmet

151015

leuargglyvalasnvalleualaaspalavallysvalthrleugly

202530

prolysglyargasnvalvalleuasplysserpheglyalaprothr

354045

ilethrlysaspglyvalservalalaarggluilegluleugluasp

505560

lysphegluasnmetglyalaglnmetvallysgluvalalaserlys

65707580

alaasnaspalaalaglyaspglythrthrthralathrvalleuala

859095

glnalaileilethrgluglyleulysalavalalaalaglymetasn

100105110

prometaspleulysargglyileasplysalavalthralaalaval

115120125

glugluleulysalaleuservalprocysseraspserlysalaile

130135140

alaglnvalglythrileseralaasnseraspgluthrvalglylys

145150155160

leuilealaglualametasplysvalglylysgluglyvalilethr

165170175

valgluaspglythrglyleuglnaspgluleuaspvalvalglugly

180185190

metglnpheaspargglytyrleuserprotyrpheileasnlyspro

195200205

gluthrglyalavalgluleugluserpropheileleuleualaasp

210215220

lyslysileserasnileargglumetleuprovalleuglualaval

225230235240

alalysalaglylysproleuleuileilealagluaspvalglugly

245250255

glualaleualathrleuvalvalasnthrmetargglyilevallys

260265270

valalaalavallysalaproglypheglyaspargarglysalamet

275280285

leuglnaspilealathrleuthrglyglythrvalilesergluglu

290295300

ileglymetgluleuglulysalathrleugluaspleuglyglnala

305310315320

lysargvalvalileasnlysaspthrthrthrileileaspglyval

325330335

glygluglualaalaileglnglyargvalalaglnileargglngln

340345350

ilegluglualathrserasptyraspargglulysleuglngluarg

355360365

valalalysleualaglyglyvalalavalilelysvalglyalaala

370375380

thrgluvalglumetlysglulyslysalaargvalgluaspalaleu

385390395400

hisalathrargalaalavalglugluglyvalvalalaglyglygly

405410415

valalaleuileargvalalaserlysleualaaspleuargglygln

420425430

asngluaspglnasnvalglyilelysvalalaleuargalametglu

435440445

alaproleuargglnilevalleuasncysglyglugluproserval

450455460

valalaasnthrvallysglyglyaspglyasntyrglytyrasnala

465470475480

alathrgluglutyrglyasnmetileaspmetglyileleuasppro

485490495

thrlysvalthrargseralaleuglntyralaalaservalalagly

500505510

leumetilethrthrglucysmetvalthraspleuprolysasnasp

515520525

alaalaaspleuglyalaalaglyglymetglyglymetglyglymet

530535540

glyglymetmet

545

<210>53

<211>474

<212>prt

<213>福氏志贺氏菌

<400>53

metserthrgluilelysthrglnvalvalvalleuglyalaglypro

151015

alaglytyrseralaalapheargcysalaaspleuglyleugluthr

202530

valilevalgluargtyrasnthrleuglyglyvalcysleuasnval

354045

glycysileproserlysalaleuleuhisvalalalysvalileglu

505560

glualalysalaleualagluhisglyilevalpheglygluprolys

65707580

thraspileasplysileargthrtrplysglulysvalileasngln

859095

leuthrglyglyleualaglymetalalysglyarglysvallysval

100105110

valasnglyleuglylysphethrglyalaasnthrleugluvalglu

115120125

glygluasnglylysthrvalileasnpheaspasnalaileileala

130135140

alaglyserargproileglnleupropheileprohisgluasppro

145150155160

argiletrpaspserthraspalaleugluleulysgluvalproglu

165170175

argleuleuvalmetglyglyglyileileglyleuglumetglythr

180185190

valtyrhisalaleuglyserglnileaspvalvalglumetpheasp

195200205

glnvalileproalaalaasplysaspilevallysvalphethrlys

210215220

argileserlyslyspheasnleumetleugluthrlysvalthrala

225230235240

valglualalysgluaspglyiletyrvalthrmetgluglylyslys

245250255

alaproalagluproglnargtyraspalavalleuvalalailegly

260265270

argvalproasnglylysasnleuaspalaglylysalaglyvalglu

275280285

valaspaspargglypheileargvalasplysglnleuargthrasn

290295300

valprohisilephealaileglyaspilevalglyglnprometleu

305310315320

alahislysglyvalhisgluglyhisvalalaalagluvalileala

325330335

glylyslyshistyrpheaspprolysvalileproserilealatyr

340345350

thrgluprogluvalalatrpvalglyleuthrglulysglualalys

355360365

glulysglyilesertyrgluthralathrpheprotrpalaalaser

370375380

glyargalailealaseraspcysalaaspglymetthrlysleuile

385390395400

pheasplysgluserhisargvalileglyglyalailevalglythr

405410415

asnglyglygluleuleuglygluileglyleualaileglumetgly

420425430

cysaspalagluaspilealaleuthrilehisalahisprothrleu

435440445

hisgluservalglyleualaalagluvalphegluglyserilethr

450455460

aspleuproasnprolysalalyslyslys

465470

<210>54

<211>112

<212>prt

<213>福氏志贺氏菌

<400>54

metasnlysasnmetalaglyileleuseralaalaalavalleuthr

151015

metleualaglycysthralatyraspargthrlysaspglnpheval

202530

glnprovalvallysaspvallyslysglymetserargalaglnval

354045

alaglnilealaglylysprosersergluvalsermetilehisala

505560

argglythrcysglnthrtyrileleuglyglnargaspglylysala

65707580

gluthrtyrphevalalaleuaspaspthrglyhisvalileasnser

859095

glytyrglnthrcysalaglutyraspthraspproglnalaalalys

100105110

<210>55

<211>239

<212>prt

<213>福氏志贺氏菌

<400>55

metalathrprohisileasnalaglumetglyaspphealaaspval

151015

valleumetproglyaspproleuargalalystyrilealagluthr

202530

pheleugluaspalaarggluvalasnasnvalargglymetleugly

354045

phethrglythrtyrlysglyarglysileservalmetglyhisgly

505560

metglyileprosercysseriletyrthrlysgluleuilethrasp

65707580

pheglyvallyslysileileargvalglysercysglyalavalleu

859095

prohisvallysleuargaspvalvalileglymetglyalacysthr

100105110

aspserlysvalasnargileargphelysasphisaspphealaala

115120125

ilealaasppheaspmetvalargasnalavalaspalaalalysala

130135140

leuglyvalaspalaargvalglyasnleupheseralaaspleuphe

145150155160

tyrserproaspglyglumetpheaspvalmetglulystyrglyile

165170175

leuglyvalglumetglualaalaglyiletyrglyvalalaalaglu

180185190

pheglyalalysalaleuthrilecysthrvalserasphisilearg

195200205

thrhisgluglnthrthralaalagluargglnthrthrpheasnasp

210215220

metilelysilealaleugluservalleuleuglyasplysglu

225230235

<210>56

<211>388

<212>prt

<213>福氏志贺氏菌

<400>56

metasnleuhisglutyrglnalalysglnleuphealaargtyrgly

151015

leuproalaprovalglytyralacysthrthrproargglualaglu

202530

glualaalaserlysileglyalaglyprotrpvalvallyscysgln

354045

valhisalaglyglyargglylysalaglyglyvallysvalvalasn

505560

serlysgluaspileargalaphealagluasntrpleuglylysarg

65707580

leuvalthrtyrglnthraspalaasnglyglnprovalasnglnile

859095

leuvalglualaalathraspilealalysgluleutyrleuglyala

100105110

valvalaspargserserargargvalvalphemetalaserthrglu

115120125

glyglyvalgluileglulysvalalaglugluthrprohisleuile

130135140

hislysvalalaleuaspproleuthrglyprometprotyrglngly

145150155160

arggluleualaphelysleuglyleugluglylysleuvalglngln

165170175

phethrlysilephemetglyleualathrilepheleugluargasp

180185190

leualaleuilegluileasnproleuvalilethrlysglnglyasp

195200205

leuilecysleuaspglylysleuglyalaaspglyasnalaleuphe

210215220

argglnproaspleuargglumetargaspglnserglngluasppro

225230235240

argglualaglnalaalaglntrpgluleuasntyrvalalaleuasp

245250255

glyasnileglycysmetvalasnglyalaglyleualametglythr

260265270

metaspilevallysleuhisglyglygluproalaasnpheleuasp

275280285

valglyglyglyalathrlysgluargvalthrglualaphelysile

290295300

ileleuseraspasplysvallysalavalleuvalasnilephegly

305310315320

glyilevalargcysaspleuilealaaspglyileileglyalaval

325330335

alagluvalglyvalasnvalprovalvalvalargleugluglyasn

340345350

asnalagluleuglyalalyslysleualaaspserglyleuasnile

355360365

ilealaalalysglyleuthraspalaalaglnglnvalvalalaala

370375380

valgluglylys

385

<210>57

<211>169

<212>prt

<213>福氏志贺氏菌

<400>57

metglyleupheasplysleulysserleuvalseraspasplyslys

151015

aspthrglythrilegluileilealaproleuserglygluileval

202530

asnilegluaspvalproaspvalvalphealaglulysilevalgly

354045

aspglyilealailelysprothrglyasnlysmetvalalaproval

505560

aspglythrileglylysilephegluthrasnhisalapheserile

65707580

gluseraspserglyvalgluleuphevalhispheglyileaspthr

859095

valgluleulysglygluglyphelysargilealaglugluglygln

100105110

argvallysvalglyaspthrvalileglupheaspleuproleuleu

115120125

gluglulysalalysserthrleuthrprovalvalileserasnmet

130135140

aspgluilelysgluleuilelysleuserglyservalthrvalgly

145150155160

gluthrprovalileargilelyslys

165

<210>58

<211>638

<212>prt

<213>福氏志贺氏菌

<400>58

metglylysileileglyileaspleuglythrthrasnsercysval

151015

alailemetaspglythrileproargvalleugluasnalaglugly

202530

aspargthrthrproserileilealatyrthrglnaspglygluthr

354045

leuvalglyglnproalalysargglnalavalthrasnproglnasn

505560

thrleuphealailelysargleuileglyargargpheglnaspglu

65707580

gluvalglnargaspvalserilemetprophelysileilealaala

859095

aspasnglyaspalatrpvalgluvallysglyglnlysmetalapro

100105110

proglnileseralagluvalleulyslysmetlyslysthralaglu

115120125

asptyrleuglygluprovalthrglualavalilethrvalproala

130135140

tyrpheasnaspalaglnargglnalathrlysaspalaglyargile

145150155160

alaglyleugluvallysargileileasngluprothralaalaala

165170175

leualatyrglyleuasplysglythrglyasnargthrilealaval

180185190

tyraspleuglyglyglythrpheaspileserileilegluileasp

195200205

gluvalaspglyglulysthrphegluvalleualathrasnglyasp

210215220

thrhisleuglyglygluasppheaspserargleuileasntyrleu

225230235240

valglugluphelyslysaspglnglyileaspleuargasnasppro

245250255

leualametglnargleulysglualaalaglulysalalysileglu

260265270

leuserseralaglnglnthraspvalasnleuprotyrilethrala

275280285

aspalathrglyprolyshismetasnilelysvalthrargalalys

290295300

leugluserleuvalgluaspleuvalasnargserilegluproleu

305310315320

lysvalalaleuglnaspalaglyleuservalseraspileaspasp

325330335

valileleuvalglyglyglnthrargmetprometvalglnlyslys

340345350

valalagluphepheglylysgluproarglysaspvalasnproasp

355360365

glualavalalaileglyalaalavalglnglyglyvalleuthrgly

370375380

aspvallysaspvalleuleuleuaspvalthrproleuserleugly

385390395400

ilegluthrmetglyglyvalmetthrthrleuilealalysasnthr

405410415

thrileprothrlyshisserglnvalpheserthralagluaspasn

420425430

glnseralavalthrilehisvalleuglnglygluarglysargala

435440445

alaaspasnlysserleuglyglnpheasnleuaspglyileasnpro

450455460

alaproargglymetproglnilegluvalthrpheaspileaspala

465470475480

aspglyileleuhisvalseralalysasplysasnserglylysglu

485490495

glnlysilethrilelysalaserserglyleuasngluaspgluile

500505510

glnlysmetvalargaspalaglualaasnalaglualaasparglys

515520525

pheglugluleuvalglnthrargasnglnglyasphisleuleuhis

530535540

serthrarglysglnvalgluglualaglyasplysleuproalaasp

545550555560

asplysthralailegluseralaleuthralaleugluthralaleu

565570575

lysglygluasplysalaalaileglualalysmetglngluleuala

580585590

glnvalserglnlysleumetgluilealaglnglnglnhisalagln

595600605

glnglnthralaglyalaaspalaseralaasnasnalalysaspasp

610615620

aspvalvalaspalaglupheglugluvallysasplyslys

625630635

<210>59

<211>190

<212>prt

<213>宋内志贺氏菌

<400>59

metlysleuvalhismetalasercysleualavalalailealaleu

151015

alaalacysalaasplysseralaaspileglnthrproalaproala

202530

alaasnthrserileseralathrglnglnproalaileglnglnpro

354045

asnvalserglythrvaltrpileargglnlysvalalaleupropro

505560

aspalavalleuthrvalthrleuseraspalaserleualaaspala

65707580

proserlysvalleualaglnlysalavalargthrgluglylysgln

859095

serpropheserphevalleupropheasnproalaaspvalglnpro

100105110

asnalaargileleuleuseralaalailethrvalasnasplysleu

115120125

valpheilethraspthrvalglnprovalileasnlysglyglythr

130135140

lysalaaspleuthrleuvalprometglnglnthralavalproval

145150155160

glnalaserglyglyalathrthrthrvalproserthrserprothr

165170175

glnvalasnproserseralavalproalaprothrglntyr

180185190

<210>60

<211>101

<212>prt

<213>福氏志贺氏菌

<400>60

metserlysgluhisthrthrgluhisleuargalagluleulysser

151015

leuseraspthrleuglugluvalleuserserserglyglulysser

202530

lysglugluleuserlysileargserlysalagluglnalaleulys

354045

glnserargtyrargleuglygluthrglyaspalailealalysgln

505560

thrargvalalaalaalaargalaaspglutyrvalarggluasnpro

65707580

trpthrglyvalglyileglyalaalaileglyvalvalleuglyval

859095

leuleuserargarg

100

<210>61

<211>330

<212>prt

<213>痢疾志贺氏菌

<400>61

metargasnileilelysleualaleuvalglyleuleuservalser

151015

thrleualavalalaalagluserserproglualaleuargilegly

202530

tyrglnlysserasnileserservalleualalysasntyrglnmet

354045

leuglulysargtyrproglnthrlysilesertrpglylysphepro

505560

alaglyproglnmetleuglualaleuasnvalglyserileaspleu

65707580

glyserthrglyaspileproproilepheserglnalaalaglyala

859095

aspleuleutyrvalservalgluproprolysprolysalagluval

100105110

ileleuvalalagluasnserproilelysthrvalalaaspleulys

115120125

glyhislysvalalapheglnlysglyserserserhisasnleuleu

130135140

leuargalaleuargglnalaglyleulysphethraspileglnpro

145150155160

thrtyrleuthrproalaaspalaargalaalapheglnglnglyasn

165170175

valaspalatrpalailetrpaspprotyrtyrseralaalaleuleu

180185190

glnglyglyvalargvalleulysaspglythraspleuasnglnthr

195200205

glyserphetyrleualaalaargprotyralaglulysasnglyala

210215220

pheileglnglyvalleualathrpheserglualaaspalaleuthr

225230235240

argserglnarggluglnserilealaleuleualalysthrmetgly

245250255

leuproalaprovalilealasertyrleuasphisargproprothr

260265270

thrilelysprovalasnalagluvalalaalaleuglnglnglnthr

275280285

alaaspleuphetyrgluasnargleuvalprolyslysvalaspile

290295300

asnalaserglyserproleuasntrplysgluasnasntyrgluser

305310315320

glutyrvalleuvalphethraspprotrp

325330

<210>62

<211>678

<212>prt

<213>宋内志贺氏菌

<400>62

metvalproserthrpheserargleulysalaalaargcysleupro

151015

valvalleualaalaleuilephealaglycysglythrhisthrpro

202530

aspglnserthralatyrmetglnglythralaglnalaaspserala

354045

phetyrleuglnglnmetglnglnserseraspaspthrargileasn

505560

trpglnleuleualaileargalaleuvallysgluglylysthrgly

65707580

glnalavalgluleupheasnglnleuproglngluleuasnaspser

859095

glnargargglulysthrleuleualavalgluilelysleualagln

100105110

lysaspphealaglyalaglnasnleuleualalysilethrproala

115120125

aspleugluglnasnglnglnalaargtyrtrpglnalalysileasp

130135140

alaserglnglyargproserileaspleuleuargalaleuileala

145150155160

glngluproleuleuglyalalysglulyslysglnasnileaspala

165170175

thrtrpglnalaleusersermetthrglngluglnalaasnthrleu

180185190

valileasnalaaspgluasnileleuglnglytrpleuaspleugln

195200205

argvaltrppheaspasnargasnaspproaspmetmetlysalagly

210215220

ilealaasptrpglnlysargtyrproasnasnproglyalalysmet

225230235240

leuprothrglnleuvalasnvallysalaphelysproalaserthr

245250255

asnlysilealaleuleuleuproleuasnglyglnalaalavalphe

260265270

glyargthrileglnglnglypheglualaalalysasnileglythr

275280285

glnprovalalaalaglnvalalaalaalaproalaalaaspvalala

290295300

gluglnproglnproglnthrvalaspglyvalalaserproalagln

305310315320

alaservalseraspleuthrglyaspglnproalaalaglnproval

325330335

provalseralaproalathrserthralaalavalseralaproala

340345350

asnproseralagluleulysiletyraspthrserserglnproleu

355360365

serglnileleuserglnvalglnglnaspglyalaserilevalval

370375380

glyproleuleulysasnasnvalglugluleuleulysserasnthr

385390395400

proleuasnvalleualaleuasnglnprogluasnilegluasnarg

405410415

valasnilecystyrphealaleuserprogluaspglualaargasp

420425430

alaalaarghisileargaspglnglylysglnalaproleuvalleu

435440445

ileproargserserleuglyaspargvalalaasnalaphealagln

450455460

glutrpglnlysleuglyglyglythrvalleuglnglnlysphegly

465470475480

serthrsergluleuargalaglyvalasnglyglyserglyileala

485490495

leuthrglythrproilethrproargalathrthraspserglymet

500505510

thrthrasnasnprothrleuglnthrthrprothraspaspglnphe

515520525

thrasnasnglyglyargvalaspalavaltyrilevalalathrpro

530535540

glygluilealapheilelysprometilealametargasnglyser

545550555560

glnserglyalametleutyralaserserargseralaglnglythr

565570575

alaglyproasppheargleuglumetgluglyleuglntyrserglu

580585590

ileprometleualaglyglyasnleuproleumetglnglnalaleu

595600605

seralavalasnasnasptyrserleualaargmettyralametgly

610615620

valaspalatrpserleualaasnhispheserglnmetargglnval

625630635640

glnglyphegluileasnglyasnthrglyserleuthralaasnpro

645650655

aspcysvalileasnarglysleusertrpleuglntyrglnglngly

660665670

glnvalvalprovalser

675

<210>63

<211>392

<212>prt

<213>福氏志贺氏菌

<400>63

metglnleuarglysleuleuleuproglyleuleuservalthrleu

151015

leuserglycysserleupheasnserglugluaspvalvallysmet

202530

serproleuprothrvalgluasnglnphethrprothrthralatrp

354045

serthrservalglyserglyileglyasnphetyrserasnleuhis

505560

proalaleualaaspasnvalvaltyralaalaaspargalaglyleu

65707580

vallysalaleuasnalaaspaspglylysgluiletrpservalser

859095

leualaglulysaspglytrppheserlysgluproalaleuleuser

100105110

glyglyvalthrvalserglyglyhisvaltyrileglyserglulys

115120125

alaglnvaltyralaleuasnthrseraspglythrvalalatrpgln

130135140

thrlysvalalaglyglualaleuserargprovalvalseraspgly

145150155160

leuvalleuilehisthrserasnglyglnleuglnalaleuasnglu

165170175

alaaspglyalavallystrpthrvalasnleuaspmetproserleu

180185190

serleuargglygluseralaproalathralapheglyalaalaval

195200205

valglyglyaspasnglyargvalseralavalleumetgluglngly

210215220

glnmetiletrpglnglnargileserglnalathrglyserthrglu

225230235240

ileaspargleuseraspvalaspthrthrprovalvalvalasngly

245250255

valvalphealaleualatyrasnglyasnleuthralaleuaspleu

260265270

argserglyglnilemettrplysarggluleuglyservalasnasp

275280285

pheilevalaspglyasnargiletyrleuvalaspglnasnasparg

290295300

valmetalaleuthrileaspglyglyvalthrleutrpalaglnser

305310315320

aspleuleuhisargleuleuthrserprovalleutyrasnglyasn

325330335

leuvalvalglyaspsergluglytyrleuhistrpileasnvalglu

340345350

aspglyargphevalalaglnglnlysvalaspserserglyphegln

355360365

thrgluprovalalaalaaspglylysleuleuileglnalalysasp

370375380

glythrvaltyrserilethrarg

385390

<210>64

<211>306

<212>prt

<213>福氏志贺氏菌

<400>64

metasnmetlysleulysthrleuphealaalaalaphealavalval

151015

glyphecysserthralaseralavalthrtyrproleuprothrasp

202530

glyserargleuvalglyglnasnglnvalilethrileproglugly

354045

asnthrglnproleuglutyrphealaalaglutyrglnmetglyleu

505560

serasnmetmetglualaasnproglyvalaspthrpheleuprolys

65707580

glyglythrvalleuasnileproglnglnleuileleuproaspthr

859095

valhisgluglyilevalileasnseralaglumetargleutyrtyr

100105110

tyrprolysglythrasnthrvalilevalleuproileglyilegly

115120125

glnleuglylysaspthrproileasntrpthrthrlysvalgluhis

130135140

lyslysalaglyprothrtrpthrprothralalysmethisalaglu

145150155160

tyrargalaalaglygluproleuproalavalvalproalaglypro

165170175

aspasnprometglyleutyralaleutyrileglyargleutyrala

180185190

ilehisglythrasnalaasnpheglyileglyleuargvalserhis

195200205

glycysvalargleuargasngluaspilelyspheleupheglulys

210215220

valprovalglythrargvalglnpheileaspgluprovallysala

225230235240

thrthrgluproaspglyserargtyrilegluvalhisasnproleu

245250255

serthrthrglualaglnphegluglyglngluilevalproilethr

260265270

leuthrlysservalglnthrvalthrglyglnproaspvalaspgln

275280285

valvalleuaspglualailelysasnargserglymetprovalarg

290295300

leuasn

305

<210>65

<211>298

<212>prt

<213>福氏志贺氏菌

<400>65

metasnvalthrvalalatyrglnthrseralagluproalalysval

151015

alaglnalaaspasnthrphealalysgluserglyalaservalasp

202530

trparglyspheaspserglyalaserilevalargalaleualaser

354045

glyaspvalglnileglyasnleuglyserserproleualavalala

505560

alaserglnglnvalproilegluvalpheleuleualaserlysleu

65707580

glyasnserglualaleuvalvallyslysthrileserlysproglu

859095

aspleuileglylysargilealavalpropheileserthrthrhis

100105110

tyrserleuleualaalaleulyshistrpglyilelysproglyleu

115120125

valgluilevalasnleuglnproproalaileilealaalatrpgln

130135140

argglyaspileaspglyalatyrvaltrpalaproalavalasnala

145150155160

leuglulysaspglylysvalleuthraspsergluglnvalglygln

165170175

trpglyalaprothrleuaspvaltrpvalvalarglysasppheala

180185190

glulyshisprogluvalvallysalaphealalysseralaileasp

195200205

alaglnglnprotyrilealaasnproaspalatrpleulysglnpro

210215220

gluasnileserlysleualaargleuserglyvalprogluglyasp

225230235240

valproglyleuvallysglyasnthrtyrleuthrproglnglngln

245250255

thralagluleuthrglyprovalasnlysalaileileaspthrala

260265270

glnpheleulysgluglnglylysvalproalavalalaasnasptyr

275280285

serglntyrvalthrserargphevalgln

290295

<210>66

<211>67

<212>prt

<213>福氏志贺氏菌

<400>66

metlysasnvalphelysalaleuthrvalleuleuthrleupheser

151015

leuthrglycysglyleulysglyproleutyrpheproproalaasp

202530

lysasnalaproproprothrlysprovalgluthrglnthrglnser

354045

thrvalproasplysasnaspargalathrglyaspglyprosergln

505560

valasntyr

65

<210>67

<211>251

<212>prt

<213>福氏志贺氏菌

<400>67

metlysleuargleuseralaleualaleuglythrthrleuleuval

151015

glycysalaserserglythraspglnglnglyargseraspproleu

202530

gluglypheasnargthrmettyrasnpheasnpheasnvalleuasp

354045

protyrilevalargprovalalavalalatrpargasptyrvalpro

505560

glnproalaargasnglyleuserasnphethrglyasnleugluglu

65707580

proalavalmetvalasntyrpheleuglnglyaspprotyrglngly

859095

metvalhisphethrargphepheleuasnthrileleuglymetgly

100105110

glypheileaspvalalaglymetalaasnprolysleuglnargthr

115120125

gluprohisargpheglyserthrleuglyhistyrglyvalglytyr

130135140

glyprotyrvalglnleuprophetyrglyserphethrleuargasp

145150155160

aspglyglyaspmetalaaspalaleutyrprovalleusertrpleu

165170175

thrtrpprometservalglylystrpthrleugluglyilegluthr

180185190

argalaglnleuleuaspseraspglyleuleuargglnserserasp

195200205

protyrilemetvalargglualatyrpheglnarghisasppheile

210215220

alaasnglyglygluleulysproglngluasnproasnalaglnala

225230235240

ileglnaspaspleulysaspileaspserglu

245250

<210>68

<211>249

<212>prt

<213>福氏志贺氏菌

<400>68

metlysargglnleuphethrleuserilevalglyvalpheserleu

151015

asnthrphealaserileproproglyasnaspvalthrthrlyspro

202530

aspleutyrtyrleuthrasnaspasnalaileaspserleualaleu

354045

leuproproproproglnileglyserilealapheleuasnaspgln

505560

alamettyrglulysglyargleuleuargasnthrgluargglylys

65707580

leualaalagluaspalaasnleuserserglyglyvalalaasnval

859095

pheseralaalapheglyserproilethralalysaspserproglu

100105110

leuhislysleuleuthrasnmetilegluaspalaglyaspleuala

115120125

thrargseralalysglutyrtyrmetargileargprophealaphe

130135140

tyrglyvalserthrcysasnthrlysgluglnaspthrleuserarg

145150155160

asnglysertyrproserglyhisthrserileglytrpalathrala

165170175

leuvalleusergluileasnproalaargglnaspthrileleulys

180185190

argglytyrgluleuglyaspserargvalilecysglytyrhistrp

195200205

glnseraspvalaspalaalaargilevalglyseralailevalala

210215220

thrleuhisserasnprovalpheglnalaglnleuglnlysalalys

225230235240

aspgluphealaasnasnglnlyslys

245

<210>69

<211>566

<212>prt

<213>福氏志贺氏菌

<400>69

metlyslyspheasnilelysserleuthrleuleuilevalleuleu

151015

proleuilevalasnalaasnasnileaspserhisleuleuglugln

202530

asnaspilealalystyrvalalaglnseraspthrvalglyserphe

354045

phegluargpheseralaleuleuasntyrproilevalvalserlys

505560

glnalaalalyslysargileserglyglupheaspleuserasnpro

65707580

gluglumetleuglulysleuthrleuleuvalglyleuiletrptyr

859095

lysaspglyasnalaleutyriletyraspserglygluleuileser

100105110

lysvalileleuleugluasnileserleuasntyrleuileglntyr

115120125

leulysaspalaasnleutyrasphisargtyrproileargglyasn

130135140

ileserasplysthrphetyrileserglyproproalaleuvalglu

145150155160

leuvalalaasnthralathrleuleuasplysglnvalserserile

165170175

glythrasplysvalasnpheglyvalilelysleulysasnthrphe

180185190

valseraspargthrtyrasnmetargglygluaspilevalilepro

195200205

glyvalalathrvalvalgluargleuleuasnasnglylysalaleu

210215220

serasnargglnalaglnasnaspprometpropropheasnilethr

225230235240

glnlysvalsergluaspserasnasppheserpheserservalthr

245250255

asnserserileleugluaspvalserleuilealatyrprogluthr

260265270

asnserileleuvallysglyasnaspglnglnileglnileilearg

275280285

aspileilethrglnleuaspvalalalysarghisilegluleuser

290295300

leutrpileileaspileasplyssergluleuasnasnleuglyval

305310315320

asntrpglnglythralaserpheglyaspserpheglyalaserphe

325330335

asnmetserserseralaserileserthrleuaspglyasnlysphe

340345350

ilealaservalmetalaleuasnglnlyslyslysalaasnvalval

355360365

serargprovalileleuthrglngluasnileproalailepheasp

370375380

asnasnargthrphetyrvalserleuvalglygluargasnserser

385390395400

leugluhisvalthrtyrglythrleuileasnvalileproargphe

405410415

serserargglyglnileglumetserleuthrilegluaspglythr

420425430

glyasnserglnserasntyrasntyrasnasngluasnthrserval

435440445

leuprogluvalglyargthrlysileserthrilealaargvalpro

450455460

glnglylysserleuleuileglyglytyrthrhisgluthrasnser

465470475480

asngluileileserilepropheleuserserileprovalilegly

485490495

asnvalphelystyrlysthrserasnileserasnilevalargval

500505510

pheleuileglnproarggluilelysglusersertyrtyrasnthr

515520525

alaglutyrlysserleuilesergluarggluileglnlysthrthr

530535540

glnileileprosergluthrthrleuleugluaspglulysserleu

545550555560

valsertyrleuasntyr

565

<210>70

<211>222

<212>prt

<213>福氏志贺氏菌

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metglyilepheserargphealaaspilevalasnalaasnileasn

151015

alaleuleuglulysalagluaspproglnlysleuvalargleumet

202530

ileglnglumetgluaspthrleuvalgluvalargserthrserala

354045

argalaleualaglulyslysglnleuthrargargilegluglnala

505560

seralaarggluvalglutrpglnglulysalagluleualaleuleu

65707580

lysgluarggluaspleualaargalaalaleuileglulysglnlys

859095

leuthraspleuilelysserleugluhisgluvalthrleuvalasp

100105110

aspthrleualaargmetlyslysgluileglygluleugluasnlys

115120125

leusergluthrargalaargglnglnalaleumetleuarghisgln

130135140

alaalaasnserserargaspvalargargglnleuaspserglylys

145150155160

leuaspglualametalaargphegluserphegluargargileasp

165170175

glnmetglualaglualagluserhisserpheglylysglnlysser

180185190

leuaspaspglnphealagluleulysalaaspaspalaileserglu

195200205

glnleualaglnleulysalalysmetlysglnaspasngln

210215220

<210>71

<211>248

<212>prt

<213>福氏志贺氏菌

<400>71

metthrlysleulysleuleualaleuglyvalleuilealathrser

151015

alaglyvalalahisalagluglylyspheserleuglyalaglyval

202530

glyvalvalgluhisprotyrlysasptyraspthraspvaltyrpro

354045

valprovalileasntyrgluglyaspasnphetrppheargglyleu

505560

glyglyglytyrtyrleutrpasnaspalathrasplysleuserile

65707580

thralatyrtrpserproleutyrphelysalalysaspserglyasp

859095

hisglnmetarghisleuaspasparglysserthrmetmetalagly

100105110

leusertyralahisphethrglntyrglytyrleuargthrthrleu

115120125

alaglyaspthrleuaspasnserasnglyilevaltrpaspmetala

130135140

trpleutyrargtyrthrasnglyglyleuthrvalthrproglyile

145150155160

glyvalglntrpasnsergluasnglnasnglutyrtyrtyrglyval

165170175

serarglysgluseralaargserglyleuargglytyrasnproasn

180185190

aspsertrpserprotyrleugluleuseralasertyrasnpheleu

195200205

glyasptrpservaltyrglythralaargtyrthrargleuserasp

210215220

gluvalthraspserprometvalasplyssertrpthrglyleuile

225230235240

serthrglyilethrtyrlysphe

245

<210>72

<211>285

<212>prt

<213>福氏志贺氏菌

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metphethrasnasnglyserthrleuglnthraspilethrthrphe

151015

glyvalasnmetlysleualahisleuglyargglnalaleumetgly

202530

valmetalavalalaleuvalalaglymetservallysserpheala

354045

aspgluglyleuleuasnlysvallysgluserglythrleuleuval

505560

glyleugluglythrtyrpropropheserpheglnglyaspaspgly

65707580

lysleuthrglyphegluvalgluphealaglnglnleualalyshis

859095

leuglyvalglualaserleulysprothrlystrpaspglymetleu

100105110

alaserleuaspserlysargileaspvalvalileasnglnvalthr

115120125

ileseraspgluarglyslyslystyrasppheserthrprotyrthr

130135140

ileserglyileglnalaleuvallyslysglyasngluglythrile

145150155160

lysthralaalaaspleulysglylyslysvalglyvalglyleugly

165170175

thrasntyrgluglutrpleuargglnasnvalglnglyvalaspval

180185190

argthrtyraspaspaspprothrlystyrglnaspleuargvalgly

195200205

argileaspalaileleuvalaspargleualaalaleuaspleuval

210215220

lyslysthrasnaspthrleualavalthrglyglualapheserarg

225230235240

glngluserglyvalalaleuarglysglyasngluaspleuleulys

245250255

alavalasnaspalailealaglumetglnlysaspglythrleugln

260265270

alaleuserglulystrppheglyalaaspvalthrlys

275280285

<210>73

<211>245

<212>prt

<213>福氏志贺氏菌

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metthrargmetlystyrleuvalalaalaalathrleuserleuphe

151015

leualaglycysserglyserlysglugluvalproaspasnpropro

202530

asngluiletyralathralaglnglnlysleuglnaspglyasntrp

354045

argglnalailethrglnleuglualaleuaspasnargtyrprophe

505560

glyprotyrserglnglnvalglnleuaspleuiletyralatyrtyr

65707580

lysasnalaaspleuproleualaglnalaalaileaspargpheile

859095

argleuasnprothrhisproasnileasptyrvalmettyrmetarg

100105110

glyleuthrasnmetalaleuaspaspseralaleuglnglyphephe

115120125

glyvalaspargseraspargaspproglnhisalaargalaalaphe

130135140

serasppheserlysleuvalargglytyrproasnserglntyrthr

145150155160

thraspalathrlysargleuvalpheleulysaspargleualalys

165170175

tyrglutyrservalalaglutyrtyrthrgluargglyalatrpval

180185190

alavalvalasnargilegluglymetleuargasptyrproaspthr

195200205

glnalathrargaspalaleuproleumetgluasnalatyrarggln

210215220

metglnmetasnalaglnalaglulysvalalalysileilealaala

225230235240

asnserserasnthr

245

<210>74

<211>1285

<212>prt

<213>福氏志贺氏菌

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metasnlysiletyrserleulystyrserhisilethrglyglyleu

151015

valalavalsergluleuthrarglysvalservalglythrserarg

202530

lyslysvalileleuglyileileleuserseriletyrglysertyr

354045

glygluthralaphealaalametleuaspileasnasniletrpthr

505560

argasptyrleuaspleualaglnasnargglyglupheargprogly

65707580

alathrasnvalglnleumetmetlysaspglylysilephehisphe

859095

progluleuprovalproasppheseralavalserasnlysglyala

100105110

thrthrserileglyglyalatyrservalthralathrhisasngly

115120125

thrglnhishisalailethrthrglnsertrpaspglnthralatyr

130135140

lysalaserasnargvalserserglyasppheservalhisargleu

145150155160

asnlysphevalvalgluthrthrglyvalthrgluseralaaspphe

165170175

serleuserprogluaspalametlysargtyrglyvalasntyrasn

180185190

glylysgluglnileileglypheargalaglyalaglythrthrser

195200205

thrileleuasnglylysglntyrleupheglyglnasntyrasnpro

210215220

aspleuleuseralaserleupheasnleuasptrplysasnlysser

225230235240

tyriletyrthrasnargthrprophelysasnserproilephegly

245250255

aspserglyserglysertyrleutyrasplysgluglnglnlystrp

260265270

valphehisglyvalthrserthrvalglypheileserserthrasn

275280285

ilealatrpthrasntyrserleupheasnasnileleuvalasnasn

290295300

leulyslysasnphethrasnthrmetglnleuaspglylyslysgln

305310315320

gluleuserserileilelysasplysaspleuservalserglygly

325330335

glyvalleuthrleulysglnaspthraspleuglyileglyglyleu

340345350

ilepheasplysasnglnthrtyrlysvaltyrglylysasplysser

355360365

tyrlysglyalaglyileaspileaspasnasnthrthrvalglutrp

370375380

asnvallysglyvalalaglyaspasnleuhislysileglysergly

385390395400

thrleuaspvallysilealaglnglyasnasnleulysileglyasn

405410415

glythrvalileleuseralaglulysalapheasnlysiletyrmet

420425430

alaglyglylysglythrvallysileasnalalysaspalaleuser

435440445

gluserglyasnglygluiletyrphethrargasnglyglythrleu

450455460

aspleuasnglytyraspglnserpheglnlysilealaalathrasp

465470475480

alaglythrthrvalthrasnserasnvallysglnserthrleuser

485490495

leuthrasnthraspalatyrmettyrhisglyasnvalserglyasn

500505510

ileserileasnhisileileasnthrthrglnglnhisasnasnasn

515520525

alaasnleuilepheaspglyservalaspilelysasnaspileser

530535540

valargasnalaglnleuthrleuglnglyhisalathrgluhisala

545550555560

ilephelysgluglyasnasnasncysproilepropheleucysgln

565570575

lysasptyrseralaalailelysaspglngluserthrvalasnlys

580585590

argtyrasnthrglutyrlysserasnasnglnilealaserpheser

595600605

glnproasptrpgluserarglyspheasnphearglysleuasnleu

610615620

gluasnalathrleuserileglyargaspalaasnvallysglyhis

625630635640

ileglualalysasnserglnilevalleuglyasnlysthralatyr

645650655

ileaspmetpheserglyargasnilethrglygluglypheglyphe

660665670

argglnglnleuargserglyaspseralaglygluserserpheasn

675680685

glyserleuseralaglnasnserlysilethrvalglyasplysser

690695700

thrvalthrmetthrglyalaleuserleuileasnthraspleuile

705710715720

ileasnlysglyalathrvalthralaglnglylysmettyrvalasp

725730735

lysalailegluleualaglythrleuthrleuthrglythrprothr

740745750

gluasnasnlystyrserproalailetyrmetseraspglytyrasn

755760765

metthrgluaspglyalathrleulysalaglnasntyralatrpval

770775780

asnglyasnilelysserasplyslysalaserileleupheglyval

785790795800

aspglntyrlysgluaspasnleuasplysthrthrhisthrproleu

805810815

alathrglyleuleuglyglypheaspthrsertyrthrglyglyile

820825830

aspalaproalaalaseralasermettyrasnthrleutrpargval

835840845

asnglyglnseralaleuglnserleulysthrargaspserleuleu

850855860

leupheserasnilegluasnserglyphehisthrvalthrvalasn

865870875880

thrleuaspalathrasnthralavalilemetargalaaspleuser

885890895

glnservalasnglnserasplysleuilevallysasnglnleuthr

900905910

glyserasnasnserleuservalaspileglnlysvalglyasnasn

915920925

asnserglyleuasnvalaspleuilethralaprolysglyserasn

930935940

lysgluilephelysalaserthrglnalaileglypheserasnile

945950955960

serprovalileserthrlysgluaspglngluhisthrthrtrpthr

965970975

leuthrglytyrlysvalalagluasnthralaserserglyalaala

980985990

lyssertyrmetserglyasntyrlysalapheleuthrgluvalasn

99510001005

asnleuasnlysargmetglyaspleuargaspthrasnglygluala

101010151020

glyalatrpalaargilemetserglyalaglyseralaserglygly

1025103010351040

tyrseraspasntyrthrhisvalglnileglyvalasplyslyshis

104510501055

gluleuaspglyleuaspleuphethrglyleuthrmetthrtyrthr

106010651070

aspserhisalaserserasnalapheserglylysthrlysserval

107510801085

glyalaglyleutyralaseralailepheaspserglyalatyrile

109010951100

aspleuileserlystyrvalhishisaspasnglutyrseralathr

1105111011151120

phealaglyleuglythrlysasptyrserserhisserleutyrval

112511301135

glyalaglualaglytyrargtyrhisvalthrgluaspsertrpile

114011451150

gluproglnalagluleuvaltyrglyalavalserglylysargphe

115511601165

asptrpglnaspargglymetservalthrmetlysasplysaspphe

117011751180

asnproleuileglyargthrglyvalaspvalglylysserpheser

1185119011951200

glylysasptrplysvalthralaargalaglyleuglytyrglnphe

120512101215

aspleuphealaasnglygluthrvalleuargaspalaserglyglu

122012251230

lysargilelysglyglulysaspglyargileleumetasnvalgly

123512401245

leuasnalagluileargaspasnleuargpheglyleuglupheglu

125012551260

lysseralapheglylystyrasnvalaspasnalaileasnalaasn

1265127012751280

pheargtyrserphe

1285

<210>75

<211>513

<212>prt

<213>福氏志贺氏菌

<400>75

metglnleuasnserthrgluilesergluleuilelysglnargile

151015

alaglnpheasnvalvalserglualahisasngluglythrileval

202530

servalseraspglyvalileargilehisglyleualaaspcysmet

354045

glnglyglumetileserleuproglyasnargtyralailealaleu

505560

asnleugluargaspservalglyalavalvalmetglyprotyrala

65707580

aspleualagluglymetlysvallyscysthrglyargileleuglu

859095

valprovalglyargglyleuleuglyargvalvalasnthrleugly

100105110

alaproileaspglylysglyproleuasphisaspglypheserala

115120125

valglualailealaproglyvalilegluargglnservalaspgln

130135140

provalglnthrglytyrlysalavalaspsermetileproilegly

145150155160

argglyglnarggluleuileileglyaspargglnthrglylysthr

165170175

alaleualaileaspalaileileasnglnargaspserglyilelys

180185190

cysiletyrvalalaileglyglnlysalaserthrileserasnval

195200205

valarglysleuglugluhisglyalaleualaasnthrilevalval

210215220

valalathralasergluseralaalaleuglntyrleualaprotyr

225230235240

alaglycysalametglyglutyrpheargaspargglygluaspala

245250255

leuileiletyraspaspleuserlysglnalavalalatyrarggln

260265270

ileserleuleuleuargargproproglyargglualapheprogly

275280285

aspvalphetyrleuhisserargleuleugluargalaalaargval

290295300

asnalaglutyrvalglualaphethrlysglygluvallysglylys

305310315320

thrglyserleuthralaleuproileilegluthrglnalaglyasp

325330335

valseralaphevalprothrasnvalileserilethraspglygln

340345350

ilepheleugluthrasnleupheasnalaglyileargproalaval

355360365

asnproglyileservalserargvalglyglyalaalaglnthrlys

370375380

ilemetlyslysleuserglyglyileargthralaleualaglntyr

385390395400

arggluleualaalapheserglnphealaseraspleuaspaspala

405410415

thrarglysglnleuasphisglyglnlysvalthrgluleuleulys

420425430

glnlysglntyralaprometservalalaglnglnserleuvalleu

435440445

phealaalagluargglytyrleualaaspvalgluleuserlysile

450455460

glyserpheglualaalaleuleualatyrvalaspargasphisala

465470475480

proleumetglngluileasnglnthrglyglytyrasnaspgluile

485490495

gluglylysleulysglyileleuaspserphelysalathrglnser

500505510

trp

<210>76

<211>469

<212>prt

<213>福氏志贺氏菌

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metseralagluhisvalleuthrmetleuasngluhisgluvallys

151015

phevalaspleuargphethraspthrlysglylysgluglnhisval

202530

thrileproalahisglnvalasnalagluphepheglugluglylys

354045

metpheaspglyserserileglyglytrplysglyileasngluser

505560

aspmetvalleumetproaspalaserthralavalileaspprophe

65707580

phealaaspserthrleuileileargcysaspileleugluprogly

859095

thrleuglnglytyraspargaspproargserilealalysargala

100105110

gluasptyrleuargserthrglyilealaaspthrvalleuphegly

115120125

progluprogluphepheleupheaspaspileargpheglyserser

130135140

ileserglyserhisvalalaileaspaspilegluglyalatrpasn

145150155160

serserthrglntyrgluglyglyasnlysglyhisargproalaval

165170175

lysglyglytyrpheprovalproprovalaspseralaglnaspile

180185190

argserglumetcysleuvalmetgluglnmetglyleuvalvalglu

195200205

alahishishisgluvalalathralaglyglnasngluvalalathr

210215220

argpheasnthrmetthrlyslysalaaspgluileglniletyrlys

225230235240

tyrvalvalhisasnvalalahisargpheglylysthralathrphe

245250255

metprolysprometpheglyaspasnglyserglymethiscyshis

260265270

metserleuserlysasnglyvalasnleuphealaglyasplystyr

275280285

alaglyleusergluglnalaleutyrtyrileglyglyvalilelys

290295300

hisalalysalaileasnalaleualaasnprothrthrasnsertyr

305310315320

lysargleuvalproglytyrglualaprovalmetleualatyrser

325330335

alaargasnargseralaserileargileprovalvalserserpro

340345350

lysalaargargilegluvalargpheproaspproalaalaasnpro

355360365

tyrleucysphealaalaleuleumetalaglyleuaspglyilelys

370375380

asnlysilehisproglyglualametasplysasnleutyraspleu

385390395400

proprogluglualalysgluileproglnvalalaglyserleuglu

405410415

glualaleuasngluleuaspleuaspargglupheleulysalagly

420425430

glyvalphethraspglualaileaspalatyrilealaleuargarg

435440445

glugluaspaspargvalargmetthrprohisprovalglupheglu

450455460

leutyrtyrserval

465

<210>77

<211>626

<212>prt

<213>福氏志贺氏菌

<400>77

metalailegluilelysvalproaspileglyalaaspgluvalglu

151015

ilethrgluileleuvallysvalglyasplysvalglualaglugln

202530

serleuilethrvalgluglyasplysalasermetgluvalproser

354045

proglnalaglyilevallysgluilelysvalservalglyasplys

505560

thrglnthrglyalaleuilemetilepheaspseralaaspglyala

65707580

alaaspalaalaproalaglnalagluglulyslysglualaalapro

859095

alaalaalaproalaalaalaalaalalysaspvalasnvalproasp

100105110

ileglyseraspgluvalgluvalthrgluileleuvallysvalgly

115120125

asplysvalglualagluglnserleuilethrvalgluglyasplys

130135140

alasermetgluvalproalaprophealaglythrvallysgluile

145150155160

lysvalasnvalglyasplysvalserthrglyserleuilemetval

165170175

phegluvalalaglyglualaglyalaalaalaproalaalalysgln

180185190

glualathrproalaalathrproalaproalaalaglyvallysglu

195200205

valasnvalproaspileglyglyaspgluvalgluvalthrgluval

210215220

metvallysvalglyasplysvalalaalagluglnserleuilethr

225230235240

valgluglyasplysalasermetgluvalproalaprophealagly

245250255

valvallysgluleulysvalasnvalglyasplysvallysthrgly

260265270

serleuilemetilephegluvalgluglyalaalaproalaalaala

275280285

proalalysglnglualaalaalaproalaproalaalalysalaglu

290295300

alaproalaalalysalagluglylyssergluphealagluasnasp

305310315320

alatyrvalhisalathrproleuileargargleualaarggluphe

325330335

glyvalasnleualalysvallysglythrglyarglysglyargile

340345350

leuarggluaspvalglnalatyrvallysglualailelysargala

355360365

glualaalaproalaalathrglyglyglyileproglymetleupro

370375380

trpprolysvalasppheserlyspheglygluileglugluvalglu

385390395400

leuglyargileglnlysileserglyalaasnleuserargasntrp

405410415

valmetileprohisvalthrhispheasplysthraspilethrglu

420425430

leuglualaphearglysglnglnasngluglualaalalysarglys

435440445

leuaspvallysilethrprovalvalpheilemetlysalavalala

450455460

alaalaleugluglnmetproargpheasnserserleusergluasp

465470475480

glyglnargleuthrleulyslystyrileasnileglyvalalaval

485490495

aspthrproasnglyleuvalvalprovalphelysaspvalasnlys

500505510

lysglyileilegluleuserarggluleumetthrileserlyslys

515520525

alaargaspglylysleuthralaglyglumetglnglyglycysphe

530535540

thrileserserileglyglyleuglythrthrhisphealaproile

545550555560

valasnalaprogluvalalaileleuglyvalserlysseralamet

565570575

gluprovaltrpasnglylysgluphevalproargleumetleupro

580585590

ileserleuserpheasphisargvalileaspglyalaaspglyala

595600605

argpheilethrileileasnasnthrleuseraspileargargleu

610615620

valmet

625

<210>78

<211>289

<212>prt

<213>福氏志贺氏菌

<400>78

metserileleuileasplysasnthrlysvalilecysglnglyphe

151015

thrglyserglnglythrphehissergluglnalailealatyrgly

202530

thrlysmetvalglyglyvalthrproglylysglyglythrthrhis

354045

leuglyleuprovalpheasnthrvalargglualavalalaalathr

505560

glyalaseralaservaliletyrvalproalaprophecyslysasp

65707580

serileleuglualaileaspalaglyilelysleuileilethrile

859095

thrgluglyileprothrleuaspmetleuthrvallysvallysleu

100105110

aspglualaglyvalargmetileglyproasncysproglyvalile

115120125

thrproglyglucyslysileglyileglnproglyhisilehislys

130135140

proglylysvalglyilevalserargserglythrleuthrtyrglu

145150155160

alavallysglnthrthrasptyrglypheglyglnserthrcysval

165170175

glyileglyglyaspproileproglyserasnpheileaspileleu

180185190

glumetpheglulysaspproglnthrglualailevalmetilegly

195200205

gluileglyglyseralaglugluglualaalaalatyrilelysglu

210215220

hisvalthrlysprovalvalglytyrilealaglyvalthralapro

225230235240

lysglylysargmetglyhisalaglyalaileilealaglyglylys

245250255

glythralaaspglulysphealaalaleuglualaalaglyvallys

260265270

thrvalargserleualaaspileglyglualaleulysthrvalleu

275280285

lys

<210>79

<211>405

<212>prt

<213>福氏志贺氏菌

<400>79

metserservalaspileleuvalproaspleuprogluservalala

151015

aspalathrvalalathrtrphislyslysproglyaspalavalval

202530

argaspgluvalleuvalgluilegluthrasplysvalvalleuglu

354045

valproalaseralaaspglyileleuaspalavalleugluaspglu

505560

glythrthrvalthrserargglnileleuglyargleuargglugly

65707580

asnseralaglylysgluthrseralalyssergluglulysalaser

859095

thrproalaglnargglnglnalaserleuglugluglnasnasnasp

100105110

alaleuserproalaileargargleuleualagluhisasnleuasp

115120125

alaseralailelysglythrglyvalglyglyargleuthrargglu

130135140

aspvalglulyshisleualalysalaproalalysgluseralapro

145150155160

alaalaalaalaproalaalaglnproalaleualaalaargserglu

165170175

lysargvalprometthrargleuarglysargvalalagluargleu

180185190

leuglualalysasnserthralametleuthrthrpheasngluval

195200205

asnmetlysproilemetaspleuarglysglntyrglyglualaphe

210215220

glulysarghisglyileargleuglyphemetserphetyrvallys

225230235240

alavalvalglualaleulysargtyrprogluvalasnalaserile

245250255

aspglyaspaspvalvaltyrhisasntyrpheaspvalsermetala

260265270

valserthrproargglyleuvalthrprovalleuargaspvalasp

275280285

thrleuglymetalaaspileglulyslysilelysgluleualaval

290295300

lysglyargaspglylysleuthrvalgluaspleuthrglyglyasn

305310315320

phethrilethrasnglyglyvalpheglyserleumetserthrpro

325330335

ileileasnproproglnseralaileleuglymethisalailelys

340345350

aspargprometalavalasnglyglnvalgluileleuprometmet

355360365

tyrleualaleusertyrasphisargleuileaspglyarggluser

370375380

valglypheleuvalthrilelysgluleuleuglugluprothrarg

385390395400

leuleuleuaspval

405

<210>80

<211>427

<212>prt

<213>福氏志贺氏菌

<400>80

metalaaspthrlysalalysleuasnleuasnglyaspthralaval

151015

gluleuaspvalleulysglythrleuglyglnaspvalileaspile

202530

argthrleuglyserlysglyvalphethrpheaspproglyphethr

354045

serthralasercysgluserlysilethrpheileaspglyaspglu

505560

glyileleuleuhisargglypheproileaspglnleualathrasp

65707580

serasntyrleugluvalcystyrileleuleuasnglyglulyspro

859095

thrglngluglntyraspgluphelysthrthrvalthrarghisthr

100105110

metilehisgluglnilethrargleuphehisalapheargargasp

115120125

serhisprometalavalmetcysglyilethrglyalaleualaala

130135140

phetyrhisaspserleuaspvalasnasnproarghisarggluile

145150155160

alaalapheargleuleuserlysmetprothrmetalaalametcys

165170175

tyrlystyrserileglyglnprophevaltyrproargasnaspleu

180185190

sertyralaglyasnpheleuasnmetmetpheserthrprocysglu

195200205

protyrgluvalasnproileleugluargalametaspargileleu

210215220

ileleuhisalaasphisgluglnasnalaserthrserthrvalarg

225230235240

thralaglyserserglyalaasnprophealacysilealaalagly

245250255

ilealaserleutrpglyproalahisglyglyalaasnglualaala

260265270

leulysmetleuglugluileserservallyshisileprogluphe

275280285

valargargalalysasplysasnaspserpheargleumetglyphe

290295300

glyhisargvaltyrlysasntyraspproargalathrvalmetarg

305310315320

gluthrcyshisgluvalleulysgluleuglythrlysaspaspleu

325330335

leugluvalalametgluleugluasnilealaleuasnaspprotyr

340345350

pheileglulyslysleutyrproasnvalaspphetyrserglyile

355360365

ileleulysalametglyileprosersermetphethrvalilephe

370375380

alametalaargthrvalglytrpilealahistrpserglumethis

385390395400

sertyrglymetlysilealaargproargglnleutyrthrglytyr

405410415

glulysargaspphelysseraspilelysarg

420425

<210>81

<211>403

<212>prt

<213>福氏志贺氏菌

<400>81

metasnthrilepheseralaargilemetlysargleualaleuthr

151015

thralaleucysthralapheileseralaalahisalaaspaspleu

202530

asnilelysthrmetileproglyvalproglnileaspalagluser

354045

tyrileleuileasptyrasnserglylysvalleualagluglnasn

505560

alaaspvalargargaspproalaserleuthrlysmetmetthrser

65707580

tyrvalileglyglnalametlysalaglylysphelysgluthrasp

859095

leuvalthrileglyasnaspalatrpalathrglyasnprovalphe

100105110

lysglyserserleumetpheleulysproglymetglnvalproval

115120125

serglnleuileargglyileasnleuglnserglyasnaspalacys

130135140

valalametalaaspphealaalaglyserglnaspalaphevalgly

145150155160

leumetasnsertyrvalasnalaleuglyleulysasnthrhisphe

165170175

glnthrvalhisglyleuaspalaaspglyglntyrserseralaarg

180185190

aspmetalaleuileglyglnalaleuileargaspvalproasnglu

195200205

tyrseriletyrlysglulysgluphethrpheasnglyilearggln

210215220

leuasnargasnglyleuleutrpaspasnserleuasnvalaspgly

225230235240

ilelysthrglyhisthrasplysalaglytyrasnleuvalalaser

245250255

alathrgluglyglnmetargleuileseralavalmetglyglyarg

260265270

thrphelysglyargglualagluserlyslysleuleuthrtrpgly

275280285

pheargphephegluthrvalasnproleulysvalglylysgluphe

290295300

alasergluprovaltrppheseraspseraspargalaserleugly

305310315320

valasplysaspvaltyrleuthrileproargglyargmetlysasp

325330335

leulysalasertyrvalleuasnsersergluleuhisalaproleu

340345350

glnlysasnglnvalvalglythrileasnpheglnleuaspglylys

355360365

thrilegluglnargproleuvalvalleuglngluileproglugly

370375380

asnphepheglylysileileasptyrilelysleumetphehishis

385390395400

trpphegly

<210>82

<211>248

<212>prt

<213>福氏志贺氏菌

<400>82

metlysservalleulysvalserleualaalaleuthrleualaphe

151015

alavalserserhisalaalaasplyslysleuvalvalalathrasp

202530

thralaphevalprophegluphelysglnglyasplystyrvalgly

354045

pheaspvalaspleutrpalaalailealalysgluleulysleuasp

505560

tyrgluleulysprometasppheserglyileileproalaleugln

65707580

thrlysasnvalaspleualaleualaglyilethrilethraspglu

859095

arglyslysalaileasppheseraspglytyrtyrlysserglyleu

100105110

leuvalmetvallysalaasnasnasnaspvallysservallysasp

115120125

leuaspglylysvalvalalavallysserglythrglyservalasp

130135140

tyralalysalaasnilelysthrlysaspleuargglnpheproasn

145150155160

ileaspasnalatyrmetgluleuglythrasnargalaaspalaval

165170175

leuhisaspthrproasnileleutyrpheilelysthralaglyasn

180185190

glyglnphelysalavalglyaspserleuglualaglnglntyrgly

195200205

ilealapheprolysglyseraspgluleuargasplysvalasngly

210215220

alaleulysthrleuarggluasnglythrtyrasngluiletyrlys

225230235240

lystrppheserthrgluprolys

245

<210>83

<211>413

<212>prt

<213>福氏志贺氏菌

<400>83

metasnlysthrleuilealaalathrvalalaglyilevalleuleu

151015

alaserasnalaglnalaglnthrvalprogluglytyrglnleugln

202530

glnvalleumetmetserarghisasnleuargalaproleualaasn

354045

asnglyservalleugluglnserthrproasnlystrpproglutrp

505560

aspvalproglyglyglnleuthrthrlysglyglyvalleugluval

65707580

tyrmetglyhistyrmetargglutrpleualagluglnglyileval

859095

lysserglyglucysproproproaspthrvaltyralatyralaasn

100105110

serleuglnargthrvalalathralaglnphepheilethrglyala

115120125

pheproglycysaspileprovalhishisglnglulysmetglythr

130135140

metaspprothrpheasnprometilethraspaspseralaalaphe

145150155160

sergluglnalavalalaalametglulysgluleuserlysleugln

165170175

leuthraspsertyrglnleuleuglulysilevalasntyrlysasp

180185190

serproalacyslysglulysglnglncysserleuvalaspglylys

195200205

asnthrpheseralalystyrglnglngluproglyvalserglypro

210215220

leulysvalglyasnserleuvalaspalaphethrleuglntyrtyr

225230235240

gluglypheprometaspglnvalalatrpglygluilelysserasp

245250255

glnglntrplysvalleuserlysleulysasnglytyrglnaspser

260265270

leuphethrserprogluvalalaargasnvalalalysproleuval

275280285

sertyrileasplysalaleuvalthraspargalaseralaprolys

290295300

ilethrvalleuvalglyhisaspserasnilealaserleuleuthr

305310315320

alaleuaspphelysprotyrglnleuhisaspglnasngluargthr

325330335

proileglyglylysilevalpheglnargtrphisaspserlysala

340345350

asnargaspleumetlysileglutyrvaltyrglnseralaglugln

355360365

leuargasnalaaspalaleuthrleuglnalaproalaglnargval

370375380

thrleugluleuserglycysproileaspalaasnglyphecyspro

385390395400

metasplyspheaspservalleuasnglualavallys

405410

<210>84

<211>891

<212>prt

<213>福氏志贺氏菌

<400>84

metalavalthrasnvalalagluleuasnalaleuvalgluargval

151015

lyslysalaglnargglutyralaserphethrglngluglnvalasp

202530

lysilepheargalaalaalaleualaalaalaaspalaargilepro

354045

leualalysmetalavalalagluserglymetglyilevalgluasp

505560

lysvalilelysasnhisphealaserglutyriletyrasnalatyr

65707580

lysaspglulysthrcysglyvalleusergluaspaspthrphegly

859095

thrilethrilealagluproileglyileilecysglyilevalpro

100105110

thrthrasnprothrserthralailephelysserleuileserleu

115120125

lysthrargasnalaileilepheserprohisproargalalysasp

130135140

alathrasnlysalaalaaspilevalleuglnalaalailealaala

145150155160

glyalaprolysaspleuileglytrpileaspglnproservalglu

165170175

leuserasnalaleumethishisproaspileasnleuileleuala

180185190

thrglyglyproglymetvallysalaalatyrserserglylyspro

195200205

alaileglyvalglyalaglyasnthrprovalvalileaspgluthr

210215220

alaaspilelysargalavalalaservalleumetserlysthrphe

225230235240

aspasnglyvalilecysalasergluglnservalvalvalvalasp

245250255

servaltyraspalavalarggluargphealathrhisglyglytyr

260265270

leuleuglnglylysgluleulysalavalglnaspvalileleulys

275280285

asnglyalaleuasnalaalailevalglyglnproalatyrlysile

290295300

alagluleualaglypheservalprogluasnthrlysileleuile

305310315320

glygluvalthrvalvalaspglusergluprophealahisglulys

325330335

leuserprothrleualamettyrargalalysaspphegluaspala

340345350

valglulysalaglulysleuvalalametglyglyileglyhisthr

355360365

sercysleutyrthraspglnaspasnglnproalaargvalsertyr

370375380

pheglyglnlysmetlysthralaargileleuileasnthrproala

385390395400

serglnglyglyileglyaspleutyrasnphelysleualaproser

405410415

leuthrleuglycysglysertrpglyglyasnserilesergluasn

420425430

valglyprolyshisleuileasnlyslysthrvalalalysargala

435440445

gluasnmetleutrphislysleuprolysseriletyrpheargarg

450455460

glyserleuproilealaleuaspgluvalilethraspglyhislys

465470475480

argalaleuilevalthraspargpheleupheasnasnglytyrala

485490495

aspglnilethrservalleulysalaalaglyvalgluthrgluval

500505510

phephegluvalglualaaspprothrleuserilevalarglysgly

515520525

alagluleualaasnserphelysproaspvalileilealaleugly

530535540

glyglyserprometaspalaalalysilemettrpvalmettyrglu

545550555560

hisprogluthrhispheglugluleualaleuargphemetaspile

565570575

arglysargiletyrlyspheprolysmetglyvallysalalysmet

580585590

ilealavalthrthrthrserglythrglysergluvalthrprophe

595600605

alavalvalthraspaspalathrglyglnlystyrproleualaasp

610615620

tyralaleuthrproaspmetalailevalaspalaasnleuvalmet

625630635640

aspmetprolysserleucysalapheglyglyleuaspalavalthr

645650655

hisalametglualatyrvalservalleualaserglupheserasp

660665670

glyglnalaleuglnalaleulysleuleulysglutyrleuproala

675680685

sertyrhisgluglyserlysasnprovalalaarggluargvalhis

690695700

seralaalathrilealaglyilealaphealaasnalapheleugly

705710715720

valcyshissermetalahislysleuglyserglnphehisilepro

725730735

hisglyleualaasnalaleuleuilecysasnvalileargtyrasn

740745750

alaasnaspasnprothrlysglnthralapheserglntyrasparg

755760765

proglnalaargargargtyralagluilealaasphisleuglyleu

770775780

seralaproglyaspargthralaalalysileglulysleuleuala

785790795800

trpleugluthrleulysalagluleuglyileprolysserilearg

805810815

glualaglyvalglnglualaasppheleualaasnvalasplysleu

820825830

sergluaspalapheaspaspglncysthrglyalaasnproargtyr

835840845

proleuilesergluleulysglnileleuleuaspthrtyrtyrgly

850855860

argasptyrvalgluaspgluthralaalalyslysglualaalapro

865870875880

alalysalaglulyslysalalyslysserala

885890

<210>85

<211>551

<212>prt

<213>福氏志贺氏菌

<400>85

metaspargargargpheilelysglysermetalametalaalaval

151015

cysglythrserglyilealaserleupheserglnalaalapheala

202530

alaaspseraspilealaaspglyglnthrglnargpheasppheser

354045

ileleuglnsermetalahisaspleualaglnthralatrparggly

505560

alaproargproleuproaspthrleualathrmetthrproglnala

65707580

tyrasnserileglntyraspalaglulysserleutrphisasnval

859095

gluasnargglnleuaspalaglnphephehismetglymetglyphe

100105110

argargargvalargmetpheservalaspproalathrhisleuala

115120125

arggluilehispheargprogluleuphelystyrasnaspalagly

130135140

valaspthrlysglnleugluglyglnseraspleuglyphealagly

145150155160

pheargvalphelysalaprogluleualaargargaspvalvalser

165170175

pheleuglyalasertyrpheargalavalaspaspthrtyrglntyr

180185190

glyleuseralaargglyleualaileaspthrtyrthraspserlys

195200205

gluglupheproaspphethralaphetrppheaspthrvallyspro

210215220

glyalathrthrphethrvaltyralaleuleuaspseralaserile

225230235240

thrglyalatyrlysphethrilehiscysglulysserglnvalile

245250255

metaspvalgluasnhisleutyralaarglysaspilelysglnleu

260265270

glyileserprometthrsermetphesercysglythrasngluarg

275280285

argmetcysaspthrilehisproglnilehisaspseraspargleu

290295300

sermettrpargglyasnglyglutrpilecysargproleuasnasn

305310315320

proglnlysleuglnpheasnalatyrthraspasnasnprolysgly

325330335

pheglyleuleuglnleuaspargasppheserhistyrglnaspile

340345350

metglytrptyrasnlysargproserleutrpvalgluproargasn

355360365

lystrpglylysglythrileglyleumetgluileprothrthrgly

370375380

gluthrleuaspasnilevalcysphetrpglnproglulysalaval

385390395400

lysalaglyaspgluphealapheglntyrargleutyrtrpserala

405410415

glnproprovalhiscysproleualaargvalmetalathrargthr

420425430

glymetglyglypheprogluglytrpalaproglygluhistyrpro

435440445

glulystrpalaargargphealavalaspphevalglyglyaspleu

450455460

lysalaalaalaprolysglyilegluprovalilethrleuserser

465470475480

glyglualalysglnilegluileleutyrilegluproileaspgly

485490495

tyrargileglnpheasptrptyrprothrseraspserthrasppro

500505510

valaspmetargmettyrleuargcysglnglyaspalaileserglu

515520525

thrtrpleutyrglntyrpheproproalaproasplysargglntyr

530535540

valaspaspargvalmetser

545550

<210>86

<211>331

<212>prt

<213>福氏志贺氏菌

<400>86

metthrilelysvalglyileasnglypheglyargileglyargile

151015

valpheargalaalaglnlysargseraspilegluilevalalaile

202530

asnaspleuleuaspalaasptyrmetalatyrmetleulystyrasp

354045

serthrhisglyargpheaspglythrvalgluvallysaspglyhis

505560

leuilevalasnglylyslysileargvalthralagluargasppro

65707580

alaasnleulystrpaspgluvalglyvalaspvalvalalagluala

859095

thrglyleupheleuthraspgluthralaarglyshisilethrala

100105110

glyalalyslysvalvalmetthrglyproserlysaspasnthrpro

115120125

metphevallysglyalaasnpheasplystyralaglyglnaspile

130135140

valserasnalasercysthrthrasncysleualaproleualalys

145150155160

valileasnaspasnpheglyileilegluglyleumetthrthrval

165170175

hisalathrthralathrglnlysthrvalaspglyproserhislys

180185190

asptrpargglyglyargglyalaserglnasnileileproserser

195200205

thrglyalaalalysalavalglylysvalleuprogluleuasngly

210215220

lysleuthrglymetalapheargvalprothrproasnvalserval

225230235240

valaspleuthrvalargleuglulysalaalathrtyrgluglnile

245250255

lysalaalavallysalaalaalagluglyglumetlysglyvalleu

260265270

glytyrthrgluaspaspvalvalserthrasppheasnglygluval

275280285

cysthrservalpheaspalalysalaglyilealaleuasnaspasn

290295300

phevallysleuvalsertrptyraspasngluthrglytyrserasn

305310315320

lysvalleuaspleuilealahisileserlys

325330

<210>87

<211>404

<212>prt

<213>福氏志贺氏菌

<400>87

metlysilevallysalagluvalphevalthrcysproglyargasn

151015

phevalthrleulysilethrthrgluaspglyilethrglyleugly

202530

aspalathrleuasnglyarggluleuservalalasertyrleugln

354045

asphisleucysproargleuileglyargaspalahisargileglu

505560

aspiletrpglnphephetyrlysglyalatyrtrpargargglypro

65707580

valthrmetseralaileseralavalaspmetalaleutrpaspile

859095

lysalalysalaalaasnmetproleutyrglnleuleuglyglyala

100105110

serarggluglyvalmetvaltyrcyshisthrthrglyhisserile

115120125

aspglualaleuaspasptyralaarghisglngluleuglyphelys

130135140

alaileargvalglncysglyileproglymetlysthrthrtyrgly

145150155160

metserlysglylysglyleualatyrgluproalathrlysglygln

165170175

trpproglugluglnleutrpserthrglulystyrleuaspphemet

180185190

prolysleupheaspalavalargasnlyspheglypheasngluhis

195200205

leuleuhisaspmethishisargleuthrproileglualaalaarg

210215220

pheglylysserilegluasptyrargmetphetrpmetgluasppro

225230235240

thrproalagluasnglnglucyspheargleuileargglnhisthr

245250255

valthrproilealavalglygluvalpheasnseriletrpaspcys

260265270

lysglnleuileglugluglnleuileasptyrileargthrthrleu

275280285

thrhisalaglyglyilethrglymetargargilealaasppheala

290295300

serleutyrglnvalargthrglyserhisglyproseraspleuser

305310315320

provalcysmetalaalaalaleuhispheaspleutrpvalproasn

325330335

pheglyvalglnglutyrmetglytyrsergluglnmetleugluval

340345350

pheprohisasntrpthrpheaspasnglytyrmethisproglyasp

355360365

lysproglyleuglyileglupheaspglulysleualaalalystyr

370375380

protyrgluproalatyrleuprovalalaargleugluaspglythr

385390395400

leutrpasnarg

<210>88

<211>406

<212>prt

<213>福氏志贺氏菌

<400>88

metthrpheservalasplysvalargalaasppheprovalleuser

151015

arggluvalasnglyleuproleualatyrleuaspseralaalaser

202530

alaglnlysproserglnvalileaspalaglualagluphetyrarg

354045

hisglytyralaalavalhisargglyilehisthrleuseralagln

505560

alathrglulysmetgluasnvalarglysargalaserleupheile

65707580

asnalaargseralaglugluleuvalphevalargglythrthrglu

859095

glyileasnleuvalalaasnsertrpglyasnserasnvalargala

100105110

glyaspasnileileileserglnmetgluhishisalaasnileval

115120125

protrpglnmetleucysalaargvalglyalagluleuargvalile

130135140

proleuasnproaspglythrleuglnleugluthrleuprothrleu

145150155160

pheaspglulysthrargleuleualailethrhisvalserasnval

165170175

leuglythrgluasnproleualaglumetilethrleualahisgln

180185190

hisglyalalysvalleuvalaspglyalaglnalavalmethishis

195200205

provalaspvalglnalaleuaspcysaspphetyrvalphesergly

210215220

hislysleutyrglyprothrglyileglyileleutyrvallysglu

225230235240

alaleuleuglnglumetproprotrpgluglyglyglysermetile

245250255

alathrvalserleusergluglythrthrtrpthrlysalaprotrp

260265270

argpheglualaglythrproasnthrglyglyileileglyleugly

275280285

alaalaleuglutyrvalseralaleuglyleuasnserilealaglu

290295300

tyrgluglnasnleumethistyralaleuserglnleugluserval

305310315320

proaspleuthrleutyrglyglnglnasnargleuglyvalileala

325330335

pheasnleuglylyshishisalatyraspvalglyserpheleuasp

340345350

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355360365

metalatyrtyrasnvalproalametcysargalaserleualamet

370375380

tyrasnthrhisglugluvalgluargleuvalthrglyleuglnarg

385390395400

ilehisargleuleugly

405

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151015

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202530

leuglulysvalglyglnleuasnproaspthrargileileglyval

354045

glyargalaasptrpasplysalaalatyrthrlysvalvalargglu

505560

alaleugluthrphemetlysgluthrileaspgluglyleutrpasp

65707580

thrleuseralaargleuaspphecysasnleuaspvalasnaspthr

859095

alaalapheasnargleuglyalametleuaspglnlysasnargile

100105110

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115120125

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130135140

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145150155160

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165170175

histyrleuglylysgluthrvalleuasnleuleualaleuargphe

180185190

alaasnserleuphevalasnasntrpaspasnargthrileasphis

195200205

valgluilethrvalalaglugluvalglyilegluglyargtrpgly

210215220

tyrpheasplysalaglyglnmetargaspmetileglnasnhisleu

225230235240

leuglnileleucysmetilealametserproproseraspleuser

245250255

alaaspserileargaspglulysvallysvalleulysserleuarg

260265270

argileaspargserasnvalargglulysthrvalargglyglntyr

275280285

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290295300

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305310315320

valaspileaspasntrpargtrpalaglyvalprophetyrleuarg

325330335

thrglylysargleuprothrlyscyssergluvalvalvaltyrphe

340345350

lysthrprogluleuasnleuphelysglusertrpglnaspleupro

355360365

glnasnlysleuthrileargleuglnproaspgluglyvalaspile

370375380

glnvalleuasnlysvalproglyleuasphislyshisasnleugln

385390395400

ilethrlysleuaspleusertyrsergluthrpheasnglnthrhis

405410415

leualaaspalatyrgluargleuleuleugluthrmetargglyile

420425430

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435440445

valaspserilethrglualatrpalametaspasnaspalaprolys

450455460

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465470475480

thrargaspglyargsertrpasnglupheglu

485490

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metargthrglutyrcysglyglnleuargleuserhisvalglygln

151015

glnvalthrleucysglytrpvalasnargargargaspleuglyser

202530

leuilepheileaspmetargasparggluglyilevalglnvalphe

354045

pheaspproaspargalaaspalaleulysleualasergluleuarg

505560

asngluphecysileglnvalthrglythrvalargalaargaspglu

65707580

lysasnileasnargaspmetalathrglygluilegluvalleuala

859095

serserleuthrileileasnargalaaspvalleuproleuaspser

100105110

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115120125

leuargargproglumetalaglnargleulysthrargalalysile

130135140

thrserleuvalargargphemetaspasphisglypheleuaspile

145150155160

gluthrprometleuthrlysalathrprogluglyalaargasptyr

165170175

leuvalproserargvalhislysglylysphetyralaleuprogln

180185190

serproglnleuphelysglnleuleumetmetserglypheasparg

195200205

tyrtyrglnilevallyscyspheargaspgluaspleuargalaasp

210215220

argglnprogluphethrglnileaspvalgluthrserphemetthr

225230235240

alaproglnvalarggluvalmetglualaleuvalarghisleutrp

245250255

leugluvallysglyvalaspleuglyasppheprovalmetthrphe

260265270

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275280285

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290295300

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305310315320

leuargvalproglyglyalaserleuthrarglysglnileaspglu

325330335

tyrglyasnphevallysiletyrglyalalysglyleualatyrile

340345350

lysvalasngluargalalysglyleugluglyileasnserproval

355360365

alalyspheleuasnalagluileileglualaileleuaspargthr

370375380

alaalaglnaspglyaspmetilephepheglyalaaspasnlyslys

385390395400

ilevalalaaspglymetglyalaleuleuleulysvalglylysasp

405410415

leuglyleuthraspgluserlystrpalaproleutrpvalileasp

420425430

pheprometleuglyaspaspglygluglyglyleuthralamethis

435440445

hisprophethrserprolysaspmetthralaalagluleulysala

450455460

alaprogluasnalavalalaasnalatyraspmetvalileasngly

465470475480

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485490495

glnthrvalpheglyileleuglyileasngluglugluglnargglu

500505510

lyspheglypheleuleuaspalaleulystyrglythrproprohis

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530535540

thraspasnileargaspvalilealapheprolysthrthralaala

545550555560

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565570575

alagluleuserileglnvalvallyslysalagluasnasn

580585590

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metleuleuglycysleualaleuthrcysserilealapheglnala

151015

seralathrglulysphelysvalilethrthrphethrileileala

202530

aspmetalalysasnvalalaglyaspalaalagluvalserserile

354045

thrlysproglyalagluilehisglutyrglnprothrproglyasp

505560

ilelysargalaglnglyalaglnleuileleualaasnglymetasn

65707580

leugluleutrppheglnargphetyrglnhisleuasnglyvalpro

859095

gluvalilevalserserglyvalthrprovalglyilethrglugly

100105110

protyrgluglylysproasnprohisalatrpmetserproaspasn

115120125

alaleuiletyrvalaspasnileargaspalaleuilelystyrasp

130135140

proalaasnalaglnthrtyrglnargasnalaaspthrtyrlysala

145150155160

lysilethrglnthrleualaproleuarglysglnilethrgluleu

165170175

progluasnglnargtrpmetvalthrsergluglyalaphesertyr

180185190

leualaargaspleuglyleulysgluleutyrleutrpproileasn

195200205

alaaspglnglnglythrproglnglnvalarglysvalvalaspile

210215220

vallyslysasnhisileproalavalphesergluserthrileser

225230235240

asplysproalaargglnvalalaarggluthrglyalahistyrgly

245250255

glyvalleutyrvalaspserleuserthrgluasnglyprovalpro

260265270

thrtyrileaspleuleulysvalthrthrserthrleuvalglngly

275280285

ilelysalaglylysargglulys

290295

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<213>福氏志贺氏菌

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metleualaleuthrasnserglycysleuasngluseraspserhis

151015

ileileargglyilelysmetgluthrthrlysproserpheglnasp

202530

valleugluphevalargleupheargarglysasnlysleuglnarg

354045

gluileglnaspvalglulyslysileargaspasnglnlysargval

505560

leuleuleuaspasnleuserasptyrilelysproglymetserval

65707580

glualaileglnglyileilealasermetlysglyasptyrgluasp

859095

argvalaspasptyrileilelysasnalagluleuserlysgluarg

100105110

argaspileserlyslysleulysalametglyglumetlysasngly

115120125

glualalys

130

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<212>prt

<213>福氏志贺氏菌

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metserglnlystyrleupheileaspargaspglythrleuileser

151015

gluproproserasppheglnvalaspargpheasplysleualaphe

202530

gluproglyvalileprogluleuleulysleuglnlysalaglytyr

354045

lysleuvalmetilethrasnglnaspglyleuglythrglnserphe

505560

proglnalaasppheaspglyprohisasnleumetmetglnilephe

65707580

thrserglnglyvalglnpheaspgluvalleuilecysprohisleu

859095

proalaaspglucysaspcysarglysprolysvallysleuvalglu

100105110

glytyrleualagluglnalametaspargalaasnsertyrvalile

115120125

glyaspargalathraspileglnleualagluasnmetglyileasn

130135140

glyleuargtyrasparggluthrleuasntrpprometileglyglu

145150155160

glnleuthrlysargaspargtyralahisvalvalargasnthrlys

165170175

gluthrglnileaspvalglnvaltrpleuasparggluglyglyser

180185190

lysileasnthrglyvalglyphepheasphismetleuaspglnile

195200205

alathrhisglyglypheargmetgluileasnvallysglyaspleu

210215220

tyrileaspasphishisthrvalgluaspthrglyleualaleugly

225230235240

glualaleulysilealaleuglyasplysargglyilecysargphe

245250255

glyphevalleuprometaspglucysleualaargcysalaleuasp

260265270

ileserglyargprohisleuglutyrlysalagluphethrtyrgln

275280285

argvalglyaspleuserthrglumetilegluhisphepheargser

290295300

leusertyrthrmetglyvalthrleuhisleulysthrlysglylys

305310315320

asnasphishisargvalgluserleuphelysalapheglyargthr

325330335

leuargglnalaileargvalgluglyaspthrleuproserserlys

340345350

glyvalleu

355

<210>94

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<212>prt

<213>福氏志贺氏菌

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metasnlyslysvalleuthrleuseralavalmetalasermetleu

151015

pheglyalaalaalahisalaalaaspthrargileglyvalthrile

202530

tyrlystyraspaspasnphemetservalvalarglysalaileglu

354045

glnaspalalysalaalaproaspvalglnleuleumetasnaspser

505560

glnasnaspglnserlysglnasnaspglnileaspvalleuleuala

65707580

lysglyvallysalaleualaileasnleuvalaspproalaalaala

859095

glythrvalileglulysalaargglyglnasnvalprovalvalphe

100105110

pheasnlysgluproserarglysalaleuaspsertyrasplysala

115120125

tyrtyrvalglythraspserlysgluserglyileileglnglyasp

130135140

leuilealalyshistrpalaalaasnglnglytrpaspleuasnlys

145150155160

aspglyglnileglnphevalleuleulysglygluproglyhispro

165170175

aspalaglualaargthrthrtyrvalilelysgluleuasnasplys

180185190

glyilelysthrgluglnleuglnleuaspthralamettrpaspthr

195200205

alaglnalalysasplysmetaspalatrpleuserglyproasnala

210215220

asnlysilegluvalvalilealaasnasnaspalametalametgly

225230235240

alavalglualaleulysalahisasnlysserserileprovalphe

245250255

glyvalaspalaleuproglualaleualaleuvallysserglyala

260265270

leualaglythrvalleuasnaspalaasnasnglnalalysalathr

275280285

pheaspleualalysasnleualaaspglylysglyalaalaaspgly

290295300

thrasntrplysileaspasnlysvalvalargvalprotyrvalgly

305310315320

valasplysaspasnleualaglupheserlyslys

325330

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<213>福氏志贺氏菌

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metserlysilevallysileileglyarggluileileaspserarg

151015

glyasnprothrvalglualagluvalhisleugluglyglypheval

202530

glymetalaalaalaproserglyalaserthrglyserarggluala

354045

leugluleuargaspglyasplysserargpheleuglylysglyval

505560

thrlysalavalalaalavalasnglyproilealaglnalaleuile

65707580

glylysaspalalysaspglnalaglyileasplysilemetileasp

859095

leuaspglythrgluasnlysserlyspheglyalaasnalaileleu

100105110

alavalserleualaasnalalysalaalaalaalaalalysglymet

115120125

proleutyrgluhisilealagluleuasnglythrproglylystyr

130135140

sermetprovalprometmetasnileileasnglyglygluhisala

145150155160

aspasnasnvalaspileglngluphemetileglnprovalglyala

165170175

lysthrvallysglualaileargmetglysergluvalphehishis

180185190

leualalysvalleulysalalysglymetasnthralavalglyasp

195200205

gluglyglytyralaproasnleuglyserasnalaglualaleuala

210215220

valilealaglualavallysalaalaglytyrgluleuglylysasp

225230235240

ilethrleualametaspcysalaalasergluphetyrlysaspgly

245250255

lystyrvalleualaglygluglyasnlysalaphethrsergluglu

260265270

phethrhispheleuglugluleuthrlysglntyrproilevalser

275280285

ilegluaspglyleuaspgluserasptrpaspglyphealatyrgln

290295300

thrlysvalleuglyasplysileglnleuvalglyaspaspleuphe

305310315320

valthrasnthrlysileleulysgluglyileglulysglyileala

325330335

asnserileleuilelyspheasnglnileglyserleuthrgluthr

340345350

leualaalailelysmetalalysaspalaglytyrthralavalile

355360365

serhisargserglygluthrgluaspalathrilealaaspleuala

370375380

valglythralaalaglyglnilelysthrglysermetserargser

385390395400

aspargvalalalystyrasnglnleuileargilegluglualaleu

405410415

glyglulysalaprotyrasnglyarglysgluilelysglyglnala

420425430

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<213>福氏志贺氏菌

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metserserarglysgluleualaasnalaileargalaleusermet

151015

aspalavalglnlysalalysserglyhisproglyalaprometgly

202530

metalaaspilealagluvalleutrpargasppheleulyshisasn

354045

proglnasnprosertrpalaaspargaspargphevalleuserasn

505560

glyhisglysermetleuiletyrserleuleuhisleuthrglytyr

65707580

aspleuprometglugluleulysasnpheargglnleuhisserlys

859095

thrproglyhisprogluvalglytyrthralaglyvalgluthrthr

100105110

thrglyproleuglyglnglyilealaasnalavalglymetalaile

115120125

alaglulysthrleualaalaglnpheasnargproglyhisaspile

130135140

valasphistyrthrtyralaphemetglyaspglycysmetmetglu

145150155160

glyileserhisgluvalcysserleualaglythrleulysleugly

165170175

lysleuilealaphetyraspaspasnglyileserileaspglyhis

180185190

valgluglytrpphethraspaspthralametargpheglualatyr

195200205

glytrphisvalileargaspileaspglyhisaspalaalaserile

210215220

lysargalavalgluglualaargalavalthrasplysproserleu

225230235240

leumetcyslysthrileileglypheglyserproasnlysalagly

245250255

thrhisaspserhisglyalaproleuglyaspalagluilealaleu

260265270

thrarggluglnleuglytrplystyralaprophegluileproser

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gluiletyralaglntrpaspalalysglualaglyglnalalysglu

290295300

seralatrpasnglulysphealaalatyralalysalatyrprogln

305310315320

glualaalagluphethrargargmetlysglyglumetproserasp

325330335

pheaspalalysalalysglupheilealalysleuglnalaasnpro

340345350

alalysilealaserarglysalaserglnasnalaileglualaphe

355360365

glyproleuleuproglupheleuglyglyseralaaspleualapro

370375380

serasnleuthrleutrpserglyserlysalaileasngluaspala

385390395400

alaglyasntyrilehistyrglyvalargglupheglymetthrala

405410415

ilealaasnglyileserleuhisglyglypheleuprotyrthrser

420425430

thrpheleumetphevalglutyralaargasnalavalargmetala

435440445

alaleumetlysglnargglnvalmetvaltyrthrhisaspserile

450455460

glyleuglygluaspglyprothrhisglnprovalgluglnvalala

465470475480

serleuargvalthrproasnmetserthrtrpargprocysaspgln

485490495

valgluseralavalalatrplystyrglyvalgluargglnaspgly

500505510

prothralaleuileleuserargglnasnleualaglnglngluarg

515520525

thrglugluglnleualaasnilealaargglyglytyrvalleulys

530535540

aspcysalaglyglnprogluleuilepheilealathrglyserglu

545550555560

valgluleualavalalaalatyrglulysleuthralagluglyval

565570575

lysalaargvalvalsermetproserthraspalapheasplysgln

580585590

aspalaalatyrarggluservalleuprolysalavalthralaarg

595600605

valalavalglualaglyilealaasptyrtrptyrlystyrvalgly

610615620

leuasnglyalailevalglymetthrthrpheglygluseralapro

625630635640

alagluleuleuphegluglupheglyphethrvalaspasnvalval

645650655

alalysalalysalaleuleu

660

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<213>福氏志贺氏菌

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metgluphephelyslysthralaleualaalaleuvalmetglyphe

151015

serglyalaalaleualaleuproasnilethrileleualathrgly

202530

glythrilealaglyglyglyaspseralathrlysserasntyrthr

354045

alaglylysvalglyvalgluasnleuvalasnalavalproglnleu

505560

lysaspilealaasnvallysglygluglnvalvalasnileglyser

65707580

glnaspmetasnaspasnvaltrpleuthrleualalyslysileasn

859095

alaaspcysasplysthraspglyphevalilethrhisglythrasp

100105110

thrmetglugluthralatyrpheleuaspleuthrvallyscysasp

115120125

lysprovalvalmetvalglyalametargproserthrsermetser

130135140

alaaspglypropheasnleutyrasnalavalvalthralaalaasp

145150155160

lysalaseralaasnargglyvalleuvalvalmetasnaspthrval

165170175

leuaspglyargaspvalthrlysthrasnthrthraspvalalathr

180185190

phelysservalasntyrglyproleuglytyrilehisasnglylys

195200205

ileasptyrglnargthrproalaarglyshisthrseraspthrpro

210215220

pheaspvalserlysleuasngluleuprolysvalglyilevaltyr

225230235240

asntyralaasnalaseraspleuproalalysalaleuvalaspala

245250255

glytyraspglyilevalseralaglyvalglyasnglyasnleutyr

260265270

lysservalpheaspthrleualathralaalalysasnglythrala

275280285

valvalargserserargvalprothrglyalathrthrglnaspala

290295300

gluvalaspaspalalystyrglyphevalalaserglythrleuasn

305310315320

proglnlysalaargvalleuleuglnleualaleuthrglnthrlys

325330335

aspproglnglnileglnglnilepheasnglntyr

340345

<210>98

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<213>福氏志贺氏菌

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metaspgluasnalaleuglyphealasertyrtrpargasnserleu

151015

alaaspalagluserglylysglyserphelysarglysaspalagln

202530

asnphethrhistrphisglyilealaalaglyargleuaspgluala

354045

ilevalserlysphephegluglyglulysaspaspvalgluthrval

505560

aspvalileleuargprolysvaltyrpheargleuleuglnhisgly

65707580

lysaspargseralaglyalaproaspilevalthrproilevalthr

859095

proalaleuleuserarggluglypheleutyrprothrproalathr

100105110

serileproargaspleuleugluproleuprolysglyalapheser

115120125

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130135140

pheserileasnpheaspaspservalasplysthralagluthrasp

145150155160

glugluargglualaargtyralaalaleuglnglnglutrparggln

165170175

tyrleutyraspsergluargleuleulysservalalaglyasptrp

180185190

ileglulysprogluglntyrgluleualagluhisglytyrileval

195200205

lysthralaglnserglyglyalaserserhisileleuserleutyr

210215220

asphisleuleuvalcysasnlysaspvalproleupheasnargphe

225230235240

alaserarggluvalhisalaalagluserleuleualaproglyala

245250255

lyspheseraspargleuglyhisserglyasplyspheproleuala

260265270

lysalaglnargaspalaleuserhispheleuaspalaarghisgly

275280285

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290295300

valleuserileilealathrglntrpalaargalaalaleuglulys

305310315320

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325330335

thrasnileileglualapheglylysasppheserglnglysergly

340345350

alametalaglyargtrpleuprogluleulysserpheglyalatyr

355360365

pheproserserserarglysalaglualaalalyslystyrglnthr

370375380

gluaspphepheasnglnvalgluserlysglutyrvalgluaspala

385390395400

leuleuphetyrleuglulysalalysalaalapheproglylysglu

405410415

cysserserproglulysvalilegluleuleuhisglyglnleuala

420425430

alalyssergluglnleuileargleuasnalathrtrpglnthrleu

435440445

serglnileargalaalaarggluleuilealaasnaspileglugln

450455460

tyrleuaspasnleuasnlysleuleuserglyglngluglnlysval

465470475480

thrleuleulysseralalysthrglutrplyslystyrargalagly

485490495

gluserleuiletyrserleuphesertrpleuproalavalargasn

500505510

lysargglntyrglnileglnleupheleugluasplysleuglyala

515520525

leuilealaglyasnglntrpseraspprogluthrilegluargasn

530535540

ileaspglyleuleuasnseralagluarggluglnthrthrtyrarg

545550555560

glnglnileaspseralahisgluilevalleulysgluglnglnala

565570575

valglnglutrpglnargleualapheaspleuglytyrgluglyasp

580585590

glugluleuserpheserglnalaaspgluleualaaspthrglnile

595600605

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354045

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115120125

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354045

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505560

argglyleuaspvallysaspleugluhisproilegluvalproval

65707580

glylysalathrleuglyargilemetasnvalleuglygluproval

859095

aspmetlysglygluileglygluglugluargtrpalailehisarg

100105110

alaalaprosertyrglugluleuserasnserglngluleuleuglu

115120125

thrglyilelysvalileaspleumetcysprophealalysglygly

130135140

lysvalglyleupheglyglyalaglyvalglylysthrvalasnmet

145150155160

metgluleuileargasnilealailegluhisserglytyrserval

165170175

phealaglyvalglygluargthrarggluglyasnaspphetyrhis

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glumetthraspserasnvalileasplysvalserleuvaltyrgly

195200205

glnmetasngluproproglyasnargleuargvalalaleuthrgly

210215220

leuthrmetalaglulyspheargaspgluglyargaspvalleuleu

225230235240

phevalaspasniletyrargtyrthrleualaglythrgluvalser

245250255

alaleuleuglyargmetproseralavalglytyrglnprothrleu

260265270

alagluglumetglyvalleuglngluargilethrserthrlysthr

275280285

glyserilethrservalglnalavaltyrvalproalaaspaspleu

290295300

thraspproserproalathrthrphealahisleuaspalathrval

305310315320

valleuserargglnilealaserleuglyiletyrproalavalasp

325330335

proleuaspserthrserargglnleuaspproleuvalvalglygln

340345350

gluhistyraspthralaargglyvalglnserileleuglnargtyr

355360365

glngluleulysaspileilealaileleuglymetaspgluleuser

370375380

glugluasplysleuvalvalalaargalaarglysileglnargphe

385390395400

leuserglnprophephevalalagluvalphethrglyserprogly

405410415

lystyrvalserleulysaspthrileargglyphelysglyilemet

420425430

gluglyglutyrasphisleuprogluglnalaphetyrmetvalgly

435440445

serilegluglualavalglulysalalyslysleu

450455460

<210>105

<211>731

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<213>福氏志贺氏菌

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metarglyslystyrmetproargalaleuglyproleuleuleuval

151015

valleuserproalavalalaglnglnasnaspaspasngluileile

202530

valseralaserargserasnargthrvalalaglumetalaglnthr

354045

thrtrpvalilegluasnalagluleugluglnglnileglnglygly

505560

lysgluleulysaspalaleualaglnleuileproglyleuaspval

65707580

serserglnserargthrasntyrglymetasnmetargglyargpro

859095

leuvalvalleuileaspglyvalargleuasnserserargserasp

100105110

serargglnleuaspservalasppropheasnileasphisileglu

115120125

valileserglyalathralaleutyrglyglyglyserthrglygly

130135140

leuileasnilevalthrlyslysglyglnprogluthrmetmetglu

145150155160

pheglualaglythrlysserglypheasnserserlysasphisasp

165170175

gluargilealaglyalavalserglyglyasnasphisilesergly

180185190

argleuservalalatyrglnlyspheglyglytrppheaspglyasn

195200205

glyaspalathrleuleuaspasnthrglnthrglyleuglnhisser

210215220

asnargleuaspilemetglythrglythrleuasnileaspgluser

225230235240

argglnleuglnleuilethrglntyrtyrlysserglnglyaspasp

245250255

asntyrglyleuasnleuglylysglypheseralaileserglyser

260265270

serthrprotyrvalserlysglyleuasnseraspargileprogly

275280285

thrgluarghisleuileserleuglntyrseraspserasppheleu

290295300

glyglngluleuvalglyglnvaltyrtyrargaspgluserleuarg

305310315320

tyrtyrpropheprothrvalasnalaasnlysglnalathralaphe

325330335

serserserglnglnaspthraspglntyrglymetlysleuthrleu

340345350

asnserglnleumetaspglytrpglnilethrtrpglyleuaspala

355360365

gluhisgluargphethrserasnglnmetphepheaspleualagln

370375380

alaseralaserglyglyleuasnasnhislysiletyrthrthrgly

385390395400

argtyrprosertyraspilethrasnleualaalapheleuglnser

405410415

sertyraspileasnaspilephethrvalserglyglyvalargtyr

420425430

glntyrthrgluasnargvalaspasppheileasptyrthrglngln

435440445

glnlysilealathrglylysalaileseralaaspalaileprogly

450455460

glyservalasptyraspasnpheleupheasnalaglyleuleumet

465470475480

hisilethrgluargglnglnalatrppheasnpheserglnglyval

485490495

alaleuproaspproglylystyrtyrglyargglyiletyrglyala

500505510

alavalasnglyhisleuproleuthrlysservalasnvalserasp

515520525

serlysleugluglyvallysvalaspsertyrgluleuglytrparg

530535540

pheileglyaspasnleuargthrglnilealaalatyrtyrserleu

545550555560

serasnlysservalgluargasnlysaspleuthrileservallys

565570575

aspaspargargargiletyrglyvalgluglyalavalasptyrleu

580585590

ileproaspthrasptrpserthrglyvalasnpheasnvalleulys

595600605

thrgluserlysvalasnglyglntrpglnlystyraspvallysglu

610615620

serserproserlysalathralatyrileasntrpalaproglupro

625630635640

trpserleuargvalglnserthrthrserpheaspvalseraspala

645650655

gluglyasnaspileasnglytyrthrthrvalasppheileserser

660665670

trpglnleuprovalglythrleuserpheservalgluasnleuphe

675680685

aspargasptyrthrthrvaltrpglyglnargalaproleutyrtyr

690695700

serproglytyrglyproalaserleutyrasptyrlysglyarggly

705710715720

argthrpheglyleuasntyrservalleuphe

725730

<210>106

<211>396

<212>prt

<213>福氏志贺氏菌

<400>106

metileileseralaalaserasptyrargalaalaalaglnargile

151015

leupropropheleuphehistyrmetaspglyglyalatyrserglu

202530

tyrthrleuargargasnvalgluaspleusergluvalalaleuarg

354045

glnargileleulysasnmetseraspleuserleugluthrthrleu

505560

pheasnglulysleusermetprovalalaleualaprovalglyleu

65707580

cysglymettyralaargargglygluvalglnalaalalysalaala

859095

aspalahisglyileprophethrleuserthrvalservalcyspro

100105110

ileglugluvalalaproalailelysargpromettrppheglnleu

115120125

tyrvalleuargaspargglyphemetargasnalaleugluargala

130135140

lysalaalaglycysserthrleuvalphethrvalaspmetprothr

145150155160

proglyalaargtyrargaspalahisserglymetserglyproasn

165170175

alaalametargargtyrleuglnalavalthrhisproglntrpala

180185190

trpaspvalglyleuasnglyargprohisaspleuglyasnileser

195200205

alatyrleuglylysprothrglyleugluasptyrileglytrpleu

210215220

glyasnasnpheaspproserilesertrplysaspleuglutrpile

225230235240

argaspphetrpaspglyprometvalilelysglyileleuasppro

245250255

gluaspalaargaspalavalargpheglyalaaspglyilevalval

260265270

serasnhisglyglyargglnleuaspglyvalleuserseralaarg

275280285

alaleuproalailealaaspalavallysglyaspilealaileleu

290295300

alaaspserglyileargasnglyleuaspvalvalargmetileala

305310315320

leuglyalaaspthrvalleuleuglyargalapheleutyralaleu

325330335

alathralaglyglnalaglyvalalaasnleuleuasnleuileglu

340345350

lysglumetlysvalalametthrleuthrglyalalysserileser

355360365

gluilethrglnaspserleuvalglnglyleuglylysgluleupro

370375380

thralaleualaprometalalysglyasnalaala

385390395

<210>107

<211>524

<212>prt

<213>福氏志贺氏菌

<400>107

metleuserthrleuargargthrleuphealaleuleualacysala

151015

serpheilevalhisalaalaalaproaspgluilethrthralatrp

202530

provalasnvalglyproleuasnprohisleutyrthrproasngln

354045

metphealaglnsermetvaltyrgluproleuvallystyrglnala

505560

aspglyservalileprotrpleualalyssertrpthrhisserglu

65707580

aspglylysthrtrpthrphethrleuargaspaspvallyspheser

859095

asnglyglupropheaspalaglualaalaalagluasnpheargala

100105110

valleuaspasnargglnarghisalatrpleugluleualaasngln

115120125

ilevalaspvallysalaleuasnlysthrgluleuglnilethrleu

130135140

lysseralatyrtyrpropheleuglngluleualaleuproargpro

145150155160

pheargpheilealaproserglnphelysasnhisgluthrmetasn

165170175

glyilelysalaproileglythrglyprotrpileleuglngluser

180185190

lysleuasnglntyraspvalphevalargasngluasntyrtrpgly

195200205

glulysproalailelyslysilethrpheasnvalileproasppro

210215220

thrthrargalavalalaphegluthrglyaspileaspleuleutyr

225230235240

glyasngluglyleuleuproleuaspthrphealaargphesergln

245250255

asnproalatyrhisthrglnleuserglnproilegluthrvalmet

260265270

leualaleuasnthralalysalaprothrasngluleualavalarg

275280285

glualaleuasntyralavalasnlyslysserleuileaspasnala

290295300

leutyrglythrglnglnvalalaaspthrleuphealaproserval

305310315320

protyralaasnleuglyleulysproargglntyraspproglnlys

325330335

alalysgluleuleuglulysalaglytrpthrleuproalaglylys

340345350

aspileargglulysasnglyglnproleuargilegluleuserphe

355360365

ileglythraspalaleuserlyssermetalagluileileglnala

370375380

aspmetargglnileglyalaaspvalserleuileglyglugluglu

385390395400

serseriletyralaargglnargaspglyargpheglymetilephe

405410415

hisargthrtrpglyalaprotyraspprohisalapheleuserser

420425430

metargvalproserhisalaasppheglnalaglnglnglyleuala

435440445

asplysproleuileasplysgluileglygluvalleualathrhis

450455460

aspgluthrglnargglnalaleutyrargaspileleuthrargleu

465470475480

hisaspglualavaltyrleuproilesertyrilesermetmetval

485490495

valserlysprogluleuglyasnileprotyralaproilealathr

500505510

gluileprophegluglnilelysprovallyspro

515520

<210>108

<211>492

<212>prt

<213>福氏志贺氏菌

<400>108

metalaalasermetvalleuvalserargalaaspalaalaglyarg

151015

glyleuservalleumetleuglualaglnaspleualacysalathr

202530

serseralaserserlysleuilehisglyglyleuargtyrleuglu

354045

histyrglupheargleuvalserglualaleualagluarggluval

505560

leuleulysmetalaprohisilealapheprometargpheargleu

65707580

prohisargprohisleuargproalatrpmetileargileglyleu

859095

phemettyrasphisleuglylysargthrserleuproglyserthr

100105110

glyleuargpheglyalaasnservalleulysprogluilelysarg

115120125

glypheglutyrseraspcystrpvalaspaspalaargleuvalleu

130135140

alaasnalaglnmetvalvalarglysglyglygluvalleuthrarg

145150155160

thrargalathrseralaargarggluasnglyleutrpilevalglu

165170175

alagluaspileaspthrglylyslystyrsertrpglnalaarggly

180185190

leuvalasnalathrglyprotrpvallysglnphepheaspglugly

195200205

methisleuproserprotyrglyileargleuilelysglyserhis

210215220

ilevalvalproargvalhisthrglnlysglnalatyrileleugln

225230235240

asngluasplysargilevalphevalileprotrpmetaspgluphe

245250255

serileileglythrthraspvalglutyrlysglyaspprolysala

260265270

vallysilegluglusergluileasntyrleuleulysvaltyrasn

275280285

alahisphelyslysglnleuserargaspaspilevaltrpthrtyr

290295300

serglyvalargproleucysaspaspgluseraspserproglnala

305310315320

ilethrargasptyrthrleuaspilehisaspgluasnglylysala

325330335

proleuleuservalpheglyglylysleuthrthrtyrarglysleu

340345350

alagluhisalaleuglulysleuthrprotyrtyrglnglyilegly

355360365

proalatrpthrlysgluservalleuproglyglyalaileglugly

370375380

aspargaspasptyralaalaargleuargargargtyrpropheleu

385390395400

thrgluserleualaarghistyrthrargthrtyrglyserasnser

405410415

gluleuleuleuglyasnalaglythrvalseraspleuglygluasp

420425430

pheglyhisgluphetyrglualagluleulystyrleuvalasphis

435440445

glutrpvalargargthraspaspalaleutrpargargthrlysgln

450455460

glymettrpleuasnalaaspglnglnserargvalserglntrpleu

465470475480

valglutyrthrglnglnlysleuserleualaser

485490

<210>109

<211>329

<212>prt

<213>福氏志贺氏菌

<400>109

metglnglyservalthrglupheleulysproargleuvalaspile

151015

gluglnvalserserthrhisalalysvalthrleugluproleuglu

202530

argglypheglyhisthrleuglyasnalaleuargargileleuleu

354045

sersermetproglycysalavalthrgluvalgluileaspglyval

505560

leuhisglutyrserthrlysgluglyvalglngluaspileleuglu

65707580

ileleuleuasnleulysglyleualavalargvalglnglylysasp

859095

gluvalileleuthrleuasnlysserglyileglyprovalthrala

100105110

alaaspilethrhisaspglyaspvalgluilevallysproglnhis

115120125

valilecyshisleuthraspgluasnalaserilesermetargile

130135140

lysvalglnargglyargglytyrvalproalaserthrargilehis

145150155160

serglugluaspgluargproileglyargleuleuvalaspalacys

165170175

tyrserprovalgluargilealatyrasnvalglualaalaargval

180185190

gluglnargthraspleuasplysleuvalileglumetgluthrasn

195200205

glythrileaspprogluglualaileargargalaalathrileleu

210215220

alagluglnleuglualaphevalaspleuargaspvalargglnpro

225230235240

gluvallysgluglulysproglupheaspproileleuleuargpro

245250255

valaspaspleugluleuthrvalargseralaasncysleulysala

260265270

glualailehistyrileglyaspleuvalglnargthrgluvalglu

275280285

leuleulysthrproasnleuglylyslysserleuthrgluilelys

290295300

aspvalleualaserargglyleuserleuglymetargleugluasn

305310315320

trpproproalaserilealaaspglu

325

<210>110

<211>102

<212>prt

<213>福氏志贺氏菌

<400>110

metalalysglyglnserleuglnasppropheleuasnalaleuarg

151015

arggluargvalprovalseriletyrleuvalasnglyilelysleu

202530

glnglyglnilegluserpheaspglnphevalileleuleulysasn

354045

thrvalserglnmetvaltyrlyshisalaileserthrvalvalpro

505560

serargprovalserhishisserasnasnalaglyglyglythrser

65707580

serasntyrhishisglyserseralaglnasnthrseralaglngln

859095

aspserglugluthrglu

100

<210>111

<211>379

<212>prt

<213>福氏志贺氏菌

<400>111

metlyslysasnilephelyspheservalleuthrleualavalleu

151015

serleuthralacysthrleuvalproglyglnasnleuserthrser

202530

asnlysaspvalilegluleuproaspasnglntyraspleuasplys

354045

metvalasniletyrprovalthrproglyleuileaspglnleuarg

505560

alalysproilemetserglnalaasnprogluleugluglnglnile

65707580

alaasntyrglutyrargileglyileglyaspvalleumetvalthr

859095

valtrpasphisprogluleuthrthrproalaglyglntyrargser

100105110

alaseraspthrglyasntrpvalasnalaaspglyalailephetyr

115120125

protyrileglyargleulysvalalaglylysthrleuthrglnval

130135140

argasngluilethralaargleuaspservalilegluserprogln

145150155160

valaspvalservalalaalapheargserglnlysalatyrvalthr

165170175

glygluvalserlysserglyglnglnproilethrasnileproleu

180185190

thrilemetaspalaileasnalaalaglyglyleuthralaaspala

195200205

asptrpargasnvalvalleuthrglnasnglyvallysthrlysval

210215220

asnleutyralaleumetglnargglyaspleuargglnasnlysleu

225230235240

leuhisproglyaspileleupheileproargasnaspaspleulys

245250255

valphevalmetglygluvalglylysglnserthrleulysmetasp

260265270

argserglymetthrleualaglualaleuglyasnalagluglymet

275280285

asnglnaspvalalaaspalathrglyilephevalileargalathr

290295300

glnasnlysglnasnglylysilealaasniletyrglnleuasnala

305310315320

lysaspalaseralametileleuglythrglupheglnleuglupro

325330335

tyraspilevaltyrvalthrthralaproleualaargtrpasnarg

340345350

valileserleuleuvalprothrileserglyvalhisaspleuthr

355360365

gluthrserargtrpileglnthrtrpproasn

370375

<210>112

<211>445

<212>prt

<213>福氏志贺氏菌

<400>112

metilethrleuarglysleuproleualavalalavalalaalagly

151015

valmetseralaglnalametalavalaspphehisglytyralaarg

202530

serglyileglytrpthrglyserglyglygluglnglncysphegln

354045

thrthrglyalaglnserlystyrargleuglyasnglucysgluthr

505560

tyralagluleulysleuglyglngluvaltrplysgluglyasplys

65707580

serphetyrpheaspthrasnvalalatyrservalalaglnglnasn

859095

asptrpglualathraspproalapheargglualaasnvalglngly

100105110

lysasnleuileglutrpleuproglyserthriletrpalaglylys

115120125

argphetyrglnarghisaspvalhismetileaspphetyrtyrtrp

130135140

aspileserglyproglyalaglyleugluasnileaspvalglyphe

145150155160

glylysleuserleualaalathrargserserglualaglyglyser

165170175

serserphealaserasnasniletyrasptyrthrasngluthrala

180185190

asnaspvalpheaspvalargleualaglnmetgluileasnprogly

195200205

glythrleugluleuglyvalasptyrglyargalaasnleuargasp

210215220

asntyrargleuvalaspglyalaserlysaspglytrpleuphethr

225230235240

alagluhisthrglnservalleulysglypheasnlysphevalval

245250255

glntyralathraspsermetthrserglnglylysglyleusergln

260265270

glyserglyvalalapheaspasnglulysphealatyrasnileasn

275280285

asnasnglyhismetleuargileleuasphisglyalailesermet

290295300

glyaspasntrpaspmetmettyrvalglymettyrglnaspileasn

305310315320

trpaspasnaspasnglythrlystrptrpthrvalglyileargpro

325330335

mettyrlystrpthrproilemetserthrvalmetgluileglytyr

340345350

aspasnvalgluserglnargthrglyasplysasnasnglntyrlys

355360365

ilethrleualaglnglntrpglnalaglyaspseriletrpserarg

370375380

proalaileargvalphealathrtyralalystrpaspglulystrp

385390395400

glytyrasptyrasnglyaspserlysvalasnproasntyrglylys

405410415

alavalproalaasppheasnglyglyserpheglyargglyaspser

420425430

aspglutrpthrpheglyalaglnmetgluiletrptrp

435440445

<210>113

<211>1262

<212>prt

<213>福氏志贺氏菌

<400>113

metalaasppheserseralathrilethrthrglyserleupropro

151015

glyasphisthrleutyrserproglntyrvalvalthralalyshis

202530

valserglyseraspthrmetserpheglytyralalysasnthrtyr

354045

thralavalglythrasnasnasnserglyleuaspilelysthrarg

505560

argleuserlysleuvalthrgluvalalaproalagluvalserasp

65707580

ileglyalavalserglyalatyrglnalaglyglyargphethrglu

859095

phetyrargleuglyglyglymetglntyrvallysasplysasngly

100105110

asnargthrglnvaltyrthrasnglyglypheleuvalglyglythr

115120125

valseralaleuasnsertyrasnasnglyglnmetilethralagln

130135140

thrglyaspilepheasnproalaasnglyproleualaasntyrleu

145150155160

asnmetglyaspserglyserproleuphealatyraspserleugln

165170175

lyslystrpvalleuileglyvalleuserserglythrasntyrgly

180185190

asnasntrpvalvalthrthrglnasppheleuglyglnglnprogln

195200205

asnasppheasplysthrilealatyrthrserglygluglyvalleu

210215220

glntrplystyraspalaalaasnglythrglythrleuthrglngly

225230235240

asnthrthrtrpaspmethisglylyslysglyasnaspleuasnala

245250255

glylysasnleuleuphethrglyasnasnglygluvalvalleugln

260265270

asnservalasnglnglyalaglytyrleuglnphealaglyasptyr

275280285

argvalseralaleuasnglyglnthrtrpmetglyglyglyileile

290295300

thrasplysglythrhisvalleutrpglnvalasnglyvalalagly

305310315320

aspasnleuhislysthrglygluglythrleuthrvalasnglythr

325330335

glyvalasnalaglyglyleulysvalglyaspglythrvalileleu

340345350

asnglnglnalaaspalaaspglylysvalglnalapheserserval

355360365

glyilealaserglyargprothrvalvalleuseraspserglngln

370375380

valasnproaspasnilesertrpglytyrargglyglyargleuglu

385390395400

leuasnglyasnasnleuthrphethrargleuglnalaalaasptyr

405410415

glyalaileilethrasnasnserglulyslysserthrvalthrleu

420425430

aspleuglnthrleulysalaseraspileasnvalprovalasnthr

435440445

valserilepheglyglyargglyalaproglyaspleutyrtyrasp

450455460

serserthrlysglntyrpheileleulysalasersertyrserpro

465470475480

phepheseraspleuasnasnserservaltrpglnasnvalglylys

485490495

asphisasnlysalaileaspthrvallysglnglnlysilegluala

500505510

serserglnprotyrmettyrhisglyglnleuasnglyasnmetasp

515520525

valasnileproglnleuserglylysaspvalleualaleuaspgly

530535540

servalasnleuprogluglyserilethrlyslysserglythrleu

545550555560

ilepheglnglyhisprovalilehisalaglythrthrthrserser

565570575

serglnserasptrpgluthrargglnphethrleuglulysleulys

580585590

leuaspalaalathrphehisleuserargasnglylysmetglngly

595600605

aspileasnalathrasnglyserthrvalileleuglyserserarg

610615620

valphethraspargseraspglythrglyasnalavalserserval

625630635640

gluglyseralathralathrthrvalglyaspglnserasptyrser

645650655

glyasnvalthrleugluasnlysserserleuglnilemetgluarg

660665670

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675680685

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690695700

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725730735

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755760765

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785790795800

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805810815

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850855860

leuseralaaspasnthrthrpheleumethisthrasnasnserarg

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885890895

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leuilethralaprolysglyseraspglulysthrphethralagly

915920925

thrglnglnileglypheserasnvalthrprovalileserthrglu

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945950955960

alaaspalaglyalaserlysalaalalysaspphemetalasergly

965970975

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980985990

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99510001005

asnglythrglyseralaaspglyasptyrseraspasntyrthrhis

101010151020

valglnileglyvalasparglyshisgluleuaspglyvalaspleu

1025103010351040

phethrglyalaleuleuthrtyrthraspserasnalaserserhis

104510501055

alapheserglylysasnlysservalglyglyglyleutyralaser

106010651070

alaleupheasnserglyalatyrpheaspleuileglylystyrleu

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hishisaspasnglnhisthralaasnphealaserleuglythrlys

109010951100

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1105111011151120

tyrhisleuthrlysglusertrpvalgluproglnilegluleuval

112511301135

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114011451150

alaleusermetlysasplysasptyrasnproleuileglyargthr

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alaargalaglyleuglytyrglnpheaspleuleualaasnglyglu

1185119011951200

thrvalleuglnaspalaserglyglulysargphegluglyglulys

120512101215

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123512401245

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151015

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202530

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354045

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provalglugluglnproglutyrproglyasnleugluleugluarg

65707580

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859095

alaserlyslysaspleugluleuglyglyhismetalaserphegln

100105110

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145150155160

gluglnleuaspasnpheargglngluvalhisglyasnglyleuser

165170175

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195200205

leulystyrleugluhisargglyleulysaspthrserlysglnthr

210215220

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225230235240

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glylysileileasngluleugluglyilephegluglyalaglytrp

275280285

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290295300

lysaspthrserglylysleuileglnleumetasngluthrvalasp

305310315320

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325330335

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340345350

thraspgluglniletrpalaleuasnargglyglyhisaspprolys

355360365

lysiletyralaalaphelyslysalaglngluthrlysglylysala

370375380

thrvalileleualahisthrilelysglytyrglymetglyaspala

385390395400

alagluglylysasnilealahisglnvallyslysmetasnmetasp

405410415

glyvalarghisileargaspargpheasnvalprovalseraspala

420425430

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450455460

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500505510

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515520525

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530535540

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545550555560

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565570575

cyssertrpleualaalaalathrsertyrserthrasnasnleupro

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610615620

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645650655

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660665670

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690695700

glyalaglugluglyilearglysglyiletyrlysleugluthrile

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725730735

arghisvalargglualaalagluileleualalysasptyrglyval

740745750

glyseraspvaltyrservalthrserphethrgluleualaargasp

755760765

glyglnaspcysgluargtrpasnmetleuhisproleugluthrpro

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argvalprotyrilealaglnvalmetasnaspalaproalavalala

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serthrasptyrmetlysleuphealagluglnvalargthrtyrval

805810815

proalaaspasptyrargvalleuglythraspglypheglyargser

820825830

aspserarggluasnleuarghishisphegluvalaspalasertyr

835840845

valvalvalalaalaleuglygluleualalysargglygluileasp

850855860

lyslysvalvalalaaspalailealalyspheasnileaspalaasp

865870875880

lysvalasnproargleuala

885

<210>115

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metalaargserlysthralaglnprolyshisserleuarglysile

151015

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202530

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354045

alaproglngluseralatrpglyproalaalathrilealaalalys

505560

hisseralathralathrlysthraspthrproileglulysthrpro

65707580

glnserileservalvalthrasngluglumetglnmetargglnphe

859095

glnservallysglualaleuglytyrthrproglyvalthrvalser

100105110

serargglyalaserasnthrtyraspphevalileileargglyphe

115120125

serservalglyleuasnglnasnasntyrleuaspglyleulysleu

130135140

glnglyasnphetyrasnaspalavalileaspprotyrmetleuglu

145150155160

argvalgluleumetargglyprothrservalleutyrglylysser

165170175

asnproglyglyileilesermetvalserlysargprothrthrglu

180185190

proleulysgluileglnphelysmetglythraspasnleuphegln

195200205

thrglypheasppheseraspalaleuaspaspasnglyglupheser

210215220

tyrargleuthrglyleualaargserthrasngluglnglnlysasn

225230235240

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245250255

aspasplysthrasnphethrpheleusertyrpheglnasnglupro

260265270

gluthrglytyrtyrglytrpleuprolysgluglythrvalglupro

275280285

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290295300

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305310315320

hisglypheasnaspthrphethrvalargglnasnleuargpheser

325330335

glumetlysthrserglnlysservaltyrglythrglyilealagly

340345350

aspglyhisthrleuasnargglythrvalvalaspasngluargleu

355360365

glnasnpheservalaspthrglnleugluserlysphealathrgly

370375380

aspvalgluhisthrleuleuthrglyvalaspphemetargmetarg

385390395400

asnaspileasnalaserpheglyseralaproserileaspleutyr

405410415

asnlystyrhisproglutyrphealapheglyseralagluprotyr

420425430

glnmetasngluserlysglnthrglyiletyrvalglnaspglnala

435440445

glutrpasnlystrpvalphethrleuglyglyargtyrasptrpser

450455460

lysglnalathrthrvalarggluasnsertyrthrprothrglugly

465470475480

tyrilegluargasnasphisglnphethrtrpargglyglyvalasn

485490495

tyrleupheaspasnglyileserprotyrphesertyrserglnser

500505510

phegluproseralapheaspleutrpserasnproargilesertyr

515520525

lysproserlysglygluglntyrglualaglyvallystyrvalpro

530535540

asnaspmetprovalvalvalthrglyalavaltyrglnleuthrlys

545550555560

thrasnasnleuthralaaspprothrasnproleualaglnvalpro

565570575

alaglygluileargalaargglyvalgluleuglualalysalaala

580585590

leuasnalaasnileasnleuthralasertyrthrtyrthraspala

595600605

glutyrthrlysaspthrasnleulysglylysthrprogluglnval

610615620

progluhismetalaserleutrpglyasptyrthrpheasnglugly

625630635640

proleuserglyleuthrleuglythrglyglyargpheileglyser

645650655

sertyrglyaspproalaasnthrphelysvalglyserthralaval

660665670

metaspalavalvallystyraspleualaargpheglymetalagly

675680685

serserleualavalasnvalasnasnleuleuaspargglutyrval

690695700

alasercyspheglnthrtyrglycysphetrpglyalagluarggln

705710715720

valvalalathralathrpheargphe

725

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<213>宋内志贺氏菌

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metasnlyslysilehisserleualaleuleuvalasnleuglyile

151015

tyrglyvalalaglnalaglngluprothraspthrprovalserhis

202530

aspaspthrilevalvalthralaalagluglnasnleuglnalapro

354045

glyvalserthrilethralaaspgluilearglysasnprovalala

505560

argaspvalsergluileileargthrmetproglyvalasnleuthr

65707580

glyasnserthrserglyglnargglyasnasnargglnileaspile

859095

argglymetglyprogluasnthrleuileleuileaspglylyspro

100105110

valserserargasnservalargglnglytrpargglygluargasp

115120125

thrargglyaspthrsertrpvalproproglumetilegluargile

130135140

gluvalleuargglyproalaalaalaargtyrglyasnglyalaala

145150155160

glyglyvalvalasnileilethrlyslysglyserglyglutrphis

165170175

glysertrpaspalatyrpheasnalaprogluhislysgluglugly

180185190

alathrlysargthrasnpheserleuthrglyproleuglyaspglu

195200205

pheserpheargleutyrglyasnleuasplysthrglnalaaspala

210215220

trpaspileasnglnglyhisglnseralaargalaglythrtyrala

225230235240

thrthrleuproalaglyarggluglyvalileasnlysaspileasn

245250255

glyvalvalargtrpaspphealaproleuglnserleugluleuglu

260265270

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275280285

thrasnseraspsertyrthrargserlystyrglyaspgluthrasn

290295300

argleutyrargglnasntyrserleuthrtrpasnglyglytrpasp

305310315320

asnglyvalthrthrserasntrpvalglntyrgluhisthrargasn

325330335

serargileprogluglyleualaglyglythrgluglylyspheasn

340345350

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355360365

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370375380

leuthrleuglythrglutrpasnglnglnargmetlysaspleuser

385390395400

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405410415

glyvalseralathraspargserprotyrserlysalagluilephe

420425430

serleuphealagluasnasnmetgluleuthraspserthrileval

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450455460

serproalaleuasnileserglnglyleuglyaspaspphethrleu

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500505510

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530535540

alaglyvalthrtrppheargasnasptyrargasnlysilegluala

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glytyrvalalavalglyglnasnalavalglythraspleutyrgln

565570575

trpaspasnvalprolysalavalvalgluglyleugluglyserleu

580585590

asnvalprovalserglumetvalmettrpthrasnasnilethrtyr

595600605

metleulyssergluasnlysthrthrglyaspargleuserileile

610615620

proglutyrthrleuasnserthrleusertrpglnalaarggluasp

625630635640

leusermetglnthrthrphethrtrptyrglylysglnglnprolys

645650655

lystyrasntyrlysglyglnproalavalglyprogluthrlysglu

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ileserprotyrserilevalglyleuseralathrtrpaspvalthr

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lysasnvalserleuthrglyglyvalaspasnleupheasplysarg

690695700

leutrpargalaglyasnalaglnthrthrglyaspleualaglyala

705710715720

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thrtrptyrmetservalasnthrhisphe

740745

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metasnmetphepheargleuthralaleualaglyleuleualaile

151015

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202530

provalleulysglugluthrglnhisalathrvalsergluargval

354045

thrserargphethrargserhistyrargglnpheaspleuaspgln

505560

alapheseralalysilepheaspargtyrleuasnleuleuasptyr

65707580

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859095

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100105110

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115120125

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130135140

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145150155160

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165170175

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180185190

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195200205

aspvalpheserleualametthralaphealaarggluileasppro

210215220

histhrasntyrleuserproargasnthrgluglnpheasnthrglu

225230235240

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245250255

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260265270

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275280285

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290295300

alaleuilelysglyprolysglyserlysvalargleugluileleu

305310315320

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325330335

argileargleugluaspargalavallysmetservallysthrval

340345350

glylysglulysvalglyvalleuaspileproglyphetyrvalgly

355360365

leuthraspaspvallysvalglnleuglnlysleuglulysglnasn

370375380

valserservalileileaspleuargserasnglyglyglyalaleu

385390395400

thrglualavalserleuserglyleupheileproalaglyproile

405410415

valglnvalargaspasnasnglylysvalarggluaspseraspthr

420425430

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435440445

pheseralaseralasergluilephealaalaalametglnasptyr

450455460

glyargalaleuvalvalglygluprothrpheglylysglythrval

465470475480

glnglntyrargserleuasnargiletyraspglnmetleuargpro

485490495

glutrpproalaleuglyservalglntyrthrileglnlysphetyr

500505510

argvalasnglyglyserthrglnarglysglyvalthrproaspile

515520525

ilemetprothrglyasnglugluthrgluthrglyglulyspheglu

530535540

aspasnalaleuprotrpaspserileaspalaalathrtyrvallys

545550555560

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565570575

alaargilealalysaspproglupheglnasnilemetlysaspile

580585590

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595600605

tyralavalargglulysgluasnasngluaspaspalathrargleu

610615620

alaargleuasngluargphelysarggluglylysprogluleulys

625630635640

lysleuaspaspleuprolysasptyrglngluproaspprotyrleu

645650655

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660665670

argproalagluglnproalaprovallys

675680

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202530

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354045

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505560

thrtyrmetalathraspasnileglyvalgluleuleualaalathr

65707580

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859095

valhishisleuproprothrleumetalaglntrptyrpheglyasp

100105110

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115120125

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130135140

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145150155160

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165170175

tyrmetaspileaspthrthralalystyrlysserglyvalthrthr

180185190

vallysaspservalargleuaspprotrpvalphemetpheserala

195200205

glytyrargphe

210

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151015

glnargilealaasnargleutyrthrserleuglyaspalaalaval

202530

glyargpheseraspglygluvalservalglnileasngluasnval

354045

argglyglyaspilepheileileglnserthrcysalaprothrasn

505560

aspasnleumetgluleuvalvalmetvalaspalaleuargargala

65707580

seralaglyargilethralavalileprotyrpheglytyralaarg

859095

glnaspargargvalargseralaargvalproilethralalysval

100105110

valalaasppheleuserservalglyvalaspargvalleuthrval

115120125

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130135140

asnvalpheglyserproileleuleugluaspmetleuglnleuasn

145150155160

leuaspasnproilevalvalserproaspileglyglyvalvalarg

165170175

alaargalailealalysleuleuasnaspthraspmetalaileile

180185190

asplysargargproargalaasnvalserglnvalmethisileile

195200205

glyaspvalalaglyargaspcysvalleuvalaspaspmetileasp

210215220

thrglyglythrleucyslysalaalaglualaleulysgluarggly

225230235240

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245250255

alaalaasnasnleuargasnservalileaspgluvalvalvalcys

260265270

aspthrileproleuseraspgluilelysserleuproasnvalarg

275280285

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290295300

asnglugluserileseralametphegluhis

305310315

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metpheargleuasnprophevalargvalglyleucysleuserala

151015

ilesercysvaltrpprovalleualavalaspaspaspglygluthr

202530

metvalvalthralaserservalgluglnasnleulysaspalapro

354045

alaserileservalilethrglngluaspleuglnarglysproval

505560

glnasnleulysaspvalleulysgluvalproglyvalglnleuthr

65707580

asngluglyaspasnarglysglyvalserileargglyleuaspser

859095

sertyrthrleuileleuvalaspglylysargvalasnserargasn

100105110

alavalphearghisasnasppheaspleuasntrpileprovalasp

115120125

serilegluargilegluvalvalargglyprometserserleutyr

130135140

glyseraspalaleuglyglyvalvalasnileilethrlyslysile

145150155160

glyglnlystrpserglythrvalthrvalaspthrthrileglnglu

165170175

hisargaspargglyaspthrtyrasnglyglnphephethrsergly

180185190

proleuileaspglyvalleuglymetlysalatyrglyserleuala

195200205

lysargglulysaspaspproglnasnserthrthrthraspthrgly

210215220

gluthrproargilegluglypheserserargaspglyasnvalglu

225230235240

phealatrpthrproasnglnasnhisaspphethralaglytyrgly

245250255

pheaspargglnaspargaspseraspserleuasplysasnargleu

260265270

gluargglnasntyrservalserhisasnglyargtrpasptyrgly

275280285

thrsergluleulystyrtyrglyglulysvalgluasnlysasnpro

290295300

glyasnserserproilethrsergluserasnthrvalaspglylys

305310315320

tyrthrleuproleuthralaileasnglnpheleuthrvalglygly

325330335

glutrparghisasplysleuseraspalavalasnleuthrglygly

340345350

thrserserlysthrseralaserglntyralaleuphevalgluasp

355360365

glutrpargilephegluproleualaleuthrthrglyvalargmet

370375380

aspasphisgluthrtyrglygluhistrpserproargalatyrleu

385390395400

valtyrasnalathraspthrvalthrvallysglyglytrpalathr

405410415

alaphelysalaproserleuleuglnleuserproasptrpthrser

420425430

asnsercysargglyalacyslysilevalglyserproaspleulys

435440445

progluthrserglusertrpgluleuglyleutyrtyrmetglyglu

450455460

gluglytrpleugluglyvalgluserservalthrvalpheargasn

465470475480

aspvallysaspargileserileserargthrseraspvalasnala

485490495

alaproglytyrglnasnphevalglyphegluthrglyalaasngly

500505510

argargileprovalphesertyrtyrasnvalasnlysalaargile

515520525

glnglyvalgluthrgluleulysilepropheasnaspglutrplys

530535540

leuserileasntyrthrtyrasnaspglyargaspvalserasngly

545550555560

gluasnlysproleuseraspleuprophehisthralaasnglythr

565570575

leuasptrplysproleualaleugluasptrpserphetyrvalser

580585590

glyhistyrthrglyglnlysargalaaspseralathralalysthr

595600605

proglyglytyrthriletrpasnthrglyalaalatrpglnvalthr

610615620

lysaspvallysleuargalaglyvalleuasnleuglyasplysasp

625630635640

leuserargaspasptyrsertyrasngluaspglyargargtyrphe

645650655

metalavalasptyrargphe

660

<210>121

<211>358

<212>prt

<213>宋内志贺氏菌

<400>121

metlysleulysleulysasnleusermetalailemetmetserthr

151015

ilevalmetglyserseralametalaalaaspserasnglulysile

202530

valilealahisargglyalaserglytyrleuprogluhisthrleu

354045

proalalysalametalatyralaglnglyalaasptyrleuglugln

505560

aspleuvalmetthrlysaspasphisleuvalvalleuhisasphis

65707580

tyrleuaspargvalthraspvalalaaspargpheproaspargala

859095

arglysaspglyargtyrtyralaileaspphethrleuaspgluile

100105110

lysserleulysphethrgluglypheaspilegluasnglylyslys

115120125

valglnthrtyrproglyargpheprometglylysseraspphearg

130135140

glyhisthrphegluglugluilegluphevalglnglyleuasnhis

145150155160

serthrglylysasnileglyiletyrprogluilelysalaprotrp

165170175

phehishisglngluglylysaspilealaalalysthrleugluval

180185190

leulyslystyrglytyrthrglylysaspasplysvaltyrleugln

195200205

cyspheaspalaaspgluleulysargilelysasngluleuglupro

210215220

lysmetglymetaspleuasnleuvalglnleuilealatyrthrasp

225230235240

trpasngluthrglnglnlysglnproaspglysertrpvalasntyr

245250255

sertyrasptrpmetphelysproglyalametlysglnvalalaglu

260265270

tyralaaspglyileglyproasptyrhismetleuileglugluthr

275280285

serglnproglyasnilelysleuthrglymetvalglnaspalagln

290295300

glnasnlysleuvalvalhisprotyrthrvalargserasplysleu

305310315320

proglutyrthrthraspvalasnglnleutyraspalaleutyrasn

325330335

lysalaglyvalasnglyleuphethrasppheproasplysalaval

340345350

lyspheleuasnlysglu

355

<210>122

<211>316

<212>prt

<213>宋内志贺氏菌

<400>122

metasngluleuaspglyilelysglnphethrthrvalvalalaasp

151015

serglyaspilegluserilearghistyrhisproglnaspalathr

202530

thrasnproserleuleuleulysalaalaglyleuserglntyrglu

354045

hisleuileaspaspalailealatrpglylyslysasnglylysthr

505560

glngluglnglnvalvalalaalacysasplysleualavalasnphe

65707580

glyalagluileleulysilevalproglyargvalserthrgluval

859095

aspalaargleuserpheasplysglulysserileglulysalaarg

100105110

hisleuvalaspleutyrglnglnglnglyvalglulysserargile

115120125

leuilelysleualaserthrtrpgluglyileargalaalagluglu

130135140

leuglulysgluglyileasncysasnleuthrleuleupheserphe

145150155160

alaglnalaargalacysalaglualaglyvalpheleuileserpro

165170175

phevalglyargiletyrasptrptyrglnalaarglysprometasp

180185190

protyrvalvalglugluaspproglyvallysservalargasnile

195200205

tyrasptyrtyrlysglnhishistyrgluthrilevalmetglyala

210215220

serpheargargthrgluglnileleualaleuthrglycysasparg

225230235240

leuthrilealaproasnleuleulysgluleuglnglulysvalser

245250255

provalvalarglysleuileproproserglnthrpheproargpro

260265270

alaprometserglualaglupheargtrpgluhisasnglntyrala

275280285

metalavalglulysleusergluglyileargleuphealavalasp

290295300

glnarglysleugluaspleuleualaalalysleu

305310315

<210>123

<211>455

<212>prt

<213>宋内志贺氏菌

<400>123

metlyslysglnproglnleuleuseralaleualaleuservalgly

151015

leuthrleuseralaserpheglnalavalalaserileproglygln

202530

valalaaspglnalaproleuproserleualaprometleuglulys

354045

valleuproalavalvalservalargvalgluglythralasergln

505560

glyglnlysileproglugluphelyslysphepheglyaspaspleu

65707580

progluglnproalaglnprophegluglyleuglyserglyvalile

859095

ileasnalaserlysglytyrvalleuthrasnasnhisvalileasn

100105110

glnalaglnlysileserileglnleuasnaspglyargglupheasp

115120125

alalysleuileglyseraspaspglnseraspilealaleuleugln

130135140

ileglnasnproserlysleuthrglnilealailealaaspserasp

145150155160

lysleuargvalglyaspphealavalalavalglyasnprophegly

165170175

leuglyglnthralathrserglyilevalseralaleuglyargser

180185190

glyleuasnleugluglyleugluasnpheileglnthraspalaser

195200205

ileasnargglyasnserglyglyalaleuleuasnleuasnglyglu

210215220

leuileglyileasnthralaileleualaproglyglyglyserval

225230235240

glyileglyphealaileproserasnmetalaargthrleualagln

245250255

glnleuileasppheglygluilelysargglyleuleuglyilelys

260265270

glythrglumetseralaaspilealalysalapheasnleuaspval

275280285

glnargglyalaphevalsergluvalleuproglyserglyserala

290295300

lysalaglyvallysalaglyaspileilethrserleuasnglylys

305310315320

proleuasnserphealagluleuargserargilealathrthrglu

325330335

proglythrlysvallysleuglyleuleuargasnglylysproleu

340345350

gluvalgluvalthrleuaspthrserthrserserseralaserala

355360365

glumetilealaproalaleugluglyalathrleuseraspglygln

370375380

leulysaspglyglylysglyilelysileaspgluvalvallysgly

385390395400

serproalaalaglnalaglyleuglnlysaspaspvalileilegly

405410415

valasnargaspargvalasnserilealaglumetarglysvalleu

420425430

alaalalysseralaileilealaleuglnilevalargglyasnglu

435440445

seriletyrleuleumetarg

450455

<210>124

<211>396

<212>prt

<213>宋内志贺氏菌

<400>124

metlysilelysthrglyalaargileleualaleuseralaleuthr

151015

thrmetmetpheseralaseralaleualalysileglugluglylys

202530

leuvaliletrpileasnglyasplysglytyrasnglyleualaglu

354045

valglylyslyspheglulysaspthrglyilelysvalthrvalglu

505560

hisproasplysleugluglulyspheproglnvalalaalathrgly

65707580

aspglyproaspileilephetrpalahisaspargpheglyglytyr

859095

alaglnserglyleuleualagluilethrproasplysalaphegln

100105110

asplysleutyrprophethrtrpaspalavalargtyrasnglylys

115120125

leuilealatyrproilealavalglualaleuserleuiletyrasn

130135140

lysaspleuleuproasnproprolysthrtrpglugluileproala

145150155160

leuasplysgluleulysalalysglylysseralaleumetpheasn

165170175

leuglngluprotyrphethrtrpproleuilealaalaaspglygly

180185190

tyralaphelystyrgluasnglylystyraspilelysaspvalala

195200205

valaspasnalaglyalalysalaglyleuthrpheleuvalaspleu

210215220

ilelysasnlyshismetasnalaaspthrasptyrserilealaglu

225230235240

alaalapheasnlysglygluthralametthrileasnglyprotrp

245250255

alatrpserasnileaspthrserlysvalasntyrglyvalthrval

260265270

leuprothrphelysglyglnproserlysprophevalglyvalleu

275280285

seralaglyileasnalaalaserproasnlysgluleualalysglu

290295300

pheleugluasntyrleuleuthraspgluglyleuglualavalasn

305310315320

lysasplysproleuglyalavalalaleulyssertyrglugluglu

325330335

leualalysaspproargilealaalathrmetgluasnalaglnlys

340345350

glygluilemetproasnileproglnmetseralaphetrptyrala

355360365

valargthralavalileasnalaalaserglyargglnthrvalasp

370375380

glualaleulysaspalaglnthrargilethrlys

385390395

<210>125

<211>290

<212>prt

<213>鲍氏志贺氏菌

<400>125

metileglyvalthraspglnlysglyglulysileleuleuaspthr

151015

thrglyproilealaargthrasnthrsertyraspglythrlysarg

202530

argasnproasnasnvalvalaspleulysasnarglystyrglncys

354045

gluglnvalasntyraspthrpheilesertyrproglnleuaspala

505560

trpseralahisproasppheglnserargileseralaglnileala

65707580

argglnvalalaleuaspargilemetileglypheasnglythrser

859095

hisalaaspgluserasnpheserthrasnlysleuleuglnaspval

100105110

asnvalglytrpleugluhisileargthraspalasergluargval

115120125

metasnaspvalthrleuthrserargasnmetaspasnthrvalala

130135140

hisalaglylystyralaasnalaaspalaleuvalglnaspalaarg

145150155160

serserleuleuaspglutrphislysglualaaspaspleuvalval

165170175

ilemetglyargasnleupheasnserleuargleuprovalleuasn

180185190

serileserglyglnasnproasnalagluleuleualaglyglnleu

195200205

ileleuserserargthrileglyglyleuglyvalpheleualapro

210215220

phepheproaspalathrmetleuilethrserpheasnasnleuser

225230235240

iletyrtrpglnlysglysermetargargleumetlysaspglupro

245250255

glutyrasnargilealathrtyrglnserileasnaspalatyrval

260265270

valgluasptyrglylyscysalametvalthrglyleulyspheala

275280285

aspser

290

<210>126

<211>375

<212>prt

<213>鲍氏志贺氏菌

<400>126

metthrileasnphecysargasnalaleuglnleuservalalaala

151015

leupheserseralaphemetalaasnalaalaaspvalproglnval

202530

lysvalthrvalthrasplysglncysgluprometthrilethrval

354045

asnalaglylysthrglnpheileileglnasnhisserglnlysala

505560

leuglutrpgluileleulysglyvalmetvalvalglugluargglu

65707580

asnilealaproglypheserglnlysmetthralaasnleuglnpro

859095

glyglutyraspmetthrcysglyleuleuthrasnprolysglylys

100105110

leuilevallysglyglualathralaaspalaalaglnseraspala

115120125

leuleuserleuglyglyalailethralatyrlysalatyrvalmet

130135140

alagluthrthrglnleuvalthraspthrlysalaphethraspala

145150155160

ilelysalaglyaspileglulysalalysalaleutyralaprothr

165170175

argglnhistyrgluargilegluproilealagluleupheserasp

180185190

leuaspglyserileaspalaarggluaspasptyrgluglnlysala

195200205

alaaspprolysphethrglyphehisargleuglulysalaleuphe

210215220

glyaspasnthrthrlysglymetaspglntyralagluglnleutyr

225230235240

thraspvalvalaspleuglnlysargilesergluleualaphepro

245250255

proserlysvalvalglyglyalaalaglyleuileglugluvalala

260265270

alaserlysileserglyglugluaspargtyrserhisthraspleu

275280285

trpasppheglnalaasnvalgluglyserglnlysilevalaspleu

290295300

leuargproglnleuglnlysalaasnprogluleuleualalysval

305310315320

aspalaasnphelyslysvalaspthrileleualalystyrargthr

325330335

lysaspglyphegluthrtyrasplysleuthraspalaaspargasn

340345350

alaleulysglyproilethralaglnalagluaspleualaglnleu

355360365

argglyvalleuglyleuasp

370375

<210>127

<211>236

<212>prt

<213>鲍氏志贺氏菌

<400>127

metlyslysglyphemetleuphethrleuleualaalaphesergly

151015

phealaglnalaaspaspalaalaileglnglnthrleualalysmet

202530

glyilelysserseraspileglnproalaprovalalaglymetlys

354045

thrvalleuthrasnserglyvalleutyrilethraspaspglylys

505560

hisileileglnglypromettyraspvalserglyalaalaproval

65707580

asnvalthrasnlysmetleuleulysglnleuasnalaleuglulys

859095

glumetilevaltyrlysalaproglnglulyshisvalilethrval

100105110

phethraspilethrcysglytyrcyshislysleuhisgluglnmet

115120125

alaasptyrasnalaleuglyilethrvalargtyrleualaphepro

130135140

argglnglyleugluserglnalagluglnglnmetlysalailetrp

145150155160

cysalalysasplysasnlysalapheaspaspvalmetalaglylys

165170175

alaalathrproalasercysaspileaspilealaasphistyrala

180185190

leuglyvalglnleuglyvalserglythrproalailevalleuser

195200205

asnglythrleuvalproglytyrglnproprolysglumetlysglu

210215220

pheleuaspgluhisglnlysmetthrserglyasn

225230235

<210>128

<211>128

<212>prt

<213>痢疾志贺氏菌

<400>128

metalaglnglnphetyrasptyrargileglnhisargserasnasp

151015

ilethralaleuargprotyrleuserasplysleualathrleuleu

202530

seraspalaserargaspasnserhisarggluleuleuserserasp

354045

propheserserargthrthrleuproaspseralahisvalalaser

505560

alaserthrileproasnargaspalaargasnileproleuargval

65707580

aspleulysglnglyaspglnglytrpglnaspgluvalleumetile

859095

glngluglyglncystrpvalileaspaspvalargtyrleuglygly

100105110

servalhisalathralaglythrleuargglnserilegluasnarg

115120125

<210>129

<211>192

<212>prt

<213>福氏志贺氏菌

<400>129

metlysargleugluhisprometlysphelysthrasnlysleuser

151015

leuasnleuvalleualaserserleuleualaalaserileproala

202530

phealavalthrglyaspthraspglnproilehisilegluserasp

354045

glnglnserleuaspmetglnglyasnvalvalthrphethrglyasn

505560

valilevalthrglnglythrilelysileasnalaasplysvalval

65707580

valthrargproglyglygluglnglylysgluvalileaspglytyr

859095

glylysproalathrphetyrglnmetglnaspasnglylysproval

100105110

gluglyhisalaserglnmethistyrgluleualalysasppheval

115120125

valleuthrglyasnalatyrleuglnglnvalaspserasnilelys

130135140

glyasplysilethrtyrleuvallysgluglnlysmetglnalaphe

145150155160

serasplysglylysargvalthrthrvalleuvalproserglnleu

165170175

glnasplysasnasnlysglyglnthrproalaglnlyslysglyasn

180185190

<210>130

<211>192

<212>prt

<213>福氏志贺氏菌

<400>130

metphelyslysileleupheproleuvalalaleuphemetleuala

151015

glycysalalysproprothrthrilegluvalserprothrilethr

202530

leuproglnglnaspproserleumetglyvalthrvalserileasn

354045

glyalaaspglnargthraspglnalaleualalysvalthrargasp

505560

asnglnilevalthrleuthralaserargalaleuargpheleuleu

65707580

glngluvalleuglulysglnmetthralaargglytyrmetvalgly

859095

proasnglyprovalasnleuglnileilevalserglnleutyrala

100105110

aspvalserglnglyasnvalargtyrasnilealathrlysalaasp

115120125

ilealaileilealathralaglnasnglyasnlysmetthrlysasn

130135140

tyrargalasertyrasnvalgluglyalapheglnalaserasnlys

145150155160

asnilealaaspalavalasnservalleuthraspthrilealaasp

165170175

metserglnaspthrserilehisglupheilelysglnasnalaarg

180185190

<210>131

<211>495

<212>prt

<213>鲍氏志贺氏菌

<400>131

metglnmetlyslysleuleuproileleuileglyleuserleuser

151015

glypheserserleuserglnalagluasnleumetglnvaltyrgln

202530

glnalaargleuserasnprogluleuarglysseralaalaasparg

354045

aspalaalapheglulysileasnglualaargserproleuleupro

505560

glnleuglyleuglyalaasptyrthrtyrserasnglytyrargasp

65707580

alaasnglyileasnserasnalathrseralaserleuglnleuala

859095

glnserilepheaspmetserlystrpargalaleuthrleuglnglu

100105110

lysalaalaglyileglnaspvalthrtyrglnthraspglnglnthr

115120125

leuileleuasnthralathralatyrpheasnvalleuasnalaile

130135140

aspvalleusertyrthrglnalaglnlysglualailetyrarggln

145150155160

leuaspglnthrthrglnargpheasnvalglyleuvalalailethr

165170175

aspvalglnasnalaargalaglntyraspthrvalleualaasnglu

180185190

valthralaargasnasnleuaspasnalavalgluglnleuarggln

195200205

ilethrglyasntyrtyrprogluleualaalaleuasnvalgluasn

210215220

phelysthrasplysproglnprovalasnalaleuleulysgluala

225230235240

glulysargasnleuserleuleuglnalaargleuserglnaspleu

245250255

alaarggluglnileargglnalaglnaspglyhisleuprothrleu

260265270

aspleuthralaserthrglyileseraspthrsertyrserglyser

275280285

lysthrargglyalaalaglythrglntyraspaspserasnmetgly

290295300

glnasnlysvalglyleuserpheserleuproiletyrglnglygly

305310315320

metvalasnserglnvallysglnalaglntyrasnphevalglyala

325330335

sergluglnleugluseralahisargserilevalglnthrvalarg

340345350

serserpheasnasnileasnalaserileserserileasnalatyr

355360365

lysglnalavalvalseralaglnserserleuaspalametgluala

370375380

glytyrservalglythrargthrilevalaspvalleuaspalathr

385390395400

thrthrleutyrasnalalysglngluleualaasnalaargtyrasn

405410415

tyrleuileasnglnleuasnilelysseralaleuglythrleuasn

420425430

gluglnaspleuleualaleuasnasnalaleuserlysprovalser

435440445

thrasnprogluasnvalalaproglnthrprogluglnasnalaile

450455460

alaaspglytyralaproaspserproalaprovalvalglnglnthr

465470475480

seralaargthrthrthrserasnglyhisasnpropheargasn

485490495

<210>132

<211>252

<212>prt

<213>鲍氏志贺氏菌

<400>132

metlysileargalaleuleuvalalametservalalathrvalleu

151015

thrglycysglnasnmetaspserasnglyleuleuserserglyala

202530

glualapheglnalatyrserleuseraspalaglnvallysthrleu

354045

seraspglnalacysglnglumetaspserlysalathrilealapro

505560

alaasnserglutyralalysargleuthrthrilealaasnalaleu

65707580

glyasnasnileasnglyglnprovalasntyrlysvaltyrmetala

859095

lysaspvalasnalaphealametalaasnglycysileargvaltyr

100105110

serglyleumetaspmetmetthraspasngluvalglualavalile

115120125

glyhisglumetglyhisvalalaleuglyhisvallyslysglymet

130135140

glnvalalaleuglythrasnalavalargvalalaalaalaserala

145150155160

glyglyilevalglyserleuserglnserglnleuglyaspleugly

165170175

glulysleuvalasnserglnpheserglnargglnglualagluala

180185190

aspasptyrsertyraspleuleuargglnargglyileserproala

195200205

glyleualathrserpheglulysleualalysleuglugluglyarg

210215220

glnsersermetpheaspasphisproalaseralagluargalagln

225230235240

hisileargaspargmetseralaaspglyilelys

245250

<210>133

<211>188

<212>prt

<213>鲍氏志贺氏菌

<400>133

metasnmetthrlysglyalaleuileleuserleuserpheleuleu

151015

alaalacysserserileproglnasnilelysglyasnasnglnpro

202530

aspileglnlysserphevalalavalhisasnglnproglyleutyr

354045

valglyglnglnalaargpheglyglylysvalileasnvalileasn

505560

glylysthraspthrleuleugluilealavalleuproleuaspser

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130135140

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202530

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354045

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505560

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65707580

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859095

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100105110

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130135140

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145150155160

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165170175

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180185190

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195200

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151015

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202530

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354045

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505560

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65707580

pheasnthrvalthrargglnaspileargalametglnaspglnval

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100105110

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115120125

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130135140

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145150155160

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165170175

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195200205

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210215220

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225230235240

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260265270

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290295300

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325330335

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340345350

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355360365

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370375380

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385390395400

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405410415

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420425430

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435440445

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450455460

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465470475480

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485490495

glnthralalysglnseraspvalvalvalalavalvalglygluala

500505510

glnglymetalahisglualaserserargthraspilethrilepro

515520525

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530535540

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545550555560

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565570575

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580585590

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595600605

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610615620

asnlystyrthrserargtyrpheaspglualaasnglyalaleutyr

625630635640

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645650655

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660665670

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690695700

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705710715720

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725730735

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740745750

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755760765

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151015

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202530

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354045

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505560

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65707580

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859095

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100105110

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115120125

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130135140

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165170175

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180185190

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195200205

glnleualaaspsertyrserlysasnvalilethrargvalilegly

210215220

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225230235240

glnglyserglnasngluserleumetservalileglutyrlysgly

245250255

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260265270

thrpheaspserglyglyileserilelysprosergluglymetasp

275280285

glumetlystyraspmetcysglyalaalaalavaltyrglyvalmet

290295300

argmetvalalagluleuglnleuproileasnvalileglyvalleu

305310315320

alaglycysgluasnmetproglyglyargalatyrargproglyasp

325330335

valleuthrthrmetserglyglnthrvalgluvalleuasnthrasp

340345350

alagluglyargleuvalleucysaspvalleuthrtyrvalgluarg

355360365

phegluproglualavalileaspvalalathrleuthrglyalacys

370375380

valilealaleuglyhishisilethrglyleumetthrasnhisasn

385390395400

proleualahisgluleuilealaalasergluglnserglyasparg

405410415

alatrpargleuproleuglyaspglutyrglngluglnleugluser

420425430

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435440445

thralaglycyspheleuserargphethrarglystyrasntrpala

450455460

hisleuaspilealaglythralatrpargserglylysalalysgly

465470475480

alathrglyargprovalalaleuleualaglnpheleuleuasnarg

485490495

alaglypheasnglygluglu

500