南昌霉素生物合成基因簇的制作方法

专利名称：南昌霉素生物合成基因簇的制作方法
技术领域：
本发明涉及的是一种抗生素生物合成基因簇，特别是一种作为聚醚类抗生素的南昌霉素生物合成基因簇，属于基因技术领域。
背景技术：
链霉菌及其近缘放线菌由于可以产生大量的天然抗生素而具有极其重要的应用价值。聚酮化合物是这些天然产物最多的类群之一，因其巨大的药用价值而被广泛地用于医用、兽用和农用等领域。例如抗细菌抗生素红霉素(erythromycin)、抗真菌抗生素两性霉素B(amphotericin B)、抗寄生虫抗生素阿维菌素(avermectin)、肿瘤抑制剂雷帕霉素(rapamycin)、抗肿瘤抗生素柔红霉素(daunarubicin)等。现有技术中还存在着一些技术问题需要解决，聚酮化合物是由聚酮合酶(PKS)催化形成的，以模块结构形式组成的PKS以类似脂肪酸生物合成的方式通过连续的缩合反应将一些简单的羧酸分子催化形成聚酮化合物。每一个模块在聚酮链形成过程中只负责一步缩合反应，它至少包含一个β-酮酯酰合成酶(KS)结构域，一个酰基转移酶(AT)结构域和一个酰基载体蛋白(ACP)结构域。此外，还可能包含一个β-酮酯酰还原酶(KR)结构域，一个脱水酶(DH)结构域和一个酯酰还原酶(ER)结构域，它们决定着加入的延伸单位的还原步骤。此外，还需要硫酯酶(TE)结构域的作用催化聚酮链的环化与释放。最后，还要经过羟基化、糖基化、甲基化和酰基化等修饰步骤。这些步骤对于大多数终产物的生物活性来说是至关重要的。
聚酮生物合成PKS的模块结构组成具有一定的可塑性，使得人们能够通过改变模块的数目、延伸模块的特异性或结构域的插入或失活等基因工程操作获得新的聚酮衍生物。聚醚抗生素是一类作用于线粒体阳离子转移的聚酮化合物，它常常具有抗微生物的活性，如抗革兰氏阳性细菌(包括分枝杆菌)和真菌。在兽药中作为一种生长促进剂也起着重要作用。聚醚抗生素的结构特点使之能够通过成环将氧原子集中在中心区域以结合合适的阳离子形成复合物，如结合钠离子的猎神霉素，侧链烷基基团然后跨越外膜。通过这种方式，阳离子便可穿越目标细胞膜，导致细胞的去极化和最终死亡。

发明内容
本发明根据背景技术中存在的不足和需要解决的技术问题，提供一种南昌霉素生物合成基因簇，其产生的一种聚醚类离子载体抗生素南昌霉素的化学结构和理化特性与猎神霉素(dianemycin)同质，对防治鸡球虫病有显著疗效，无毒副作用，同时还具有一定的增重效果，是一种理想的抗鸡球虫药。
本发明是通过以下的技术方案来实现的，本发明整个聚醚类离子载体抗生素生物合成基因簇共30个基因的核苷酸序列或互补序列(序列1)。其中11个用于编码聚酮合酶(PKS)，它包含14个模块，共74个结构域，负责催化南昌霉素聚酮糖苷配基的生物合成。另有4个基因，即nanE，nanI，nanO，nanP编码参与南昌霉素生物合成修饰的蛋白，负责催化聚酮链的氧化、异构化和聚醚结构的形成。还有6个基因，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM负责编码参与南昌霉素生物合成中脱氧糖(4-O-甲基-L-rhodinose)的合成及转移的蛋白。此外，在基因簇的两侧还存在着两组共9个调节基因，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5，它们参与南昌霉素生物合成的调节。这些核苷酸序列是分别选自于序列1中的nanT1(2052-682)，nanT2(2064-2879)，nanT3(3117-3818)，nanT4(4923-3724)，nanT5(6106-4916)，nanR1(7493-6768)，nanR2(8334-7573)，nanA1(8919-17627)，nanA2(17642-24313)，nanA3(24310-36408)，nanA4(36429-48299)，nanA5(48345-60284)，nanA6(60305-65302)，nanG5(66747-65365)，nanM(66881-67798)，nanG4(67823-68863)，nanG3(68860-70164)，nanG2(71140-70145)，nanG1(72045-71137)，nanA7(76990-72050)，nanE(77864-76992)，nanA10(78263-77949)，nanO(79763-78327)，nanI(80769-79828)，nanA9(83192-80784)，nanA11(89759-83196)，nanA8(100138-89771)，nanP(101467-100196)，nanR3(101593-102600)，nanR4(102600-103541)。
本发明还提供了一个编码膜整合型转移蛋白的核苷酸序列，由序列2中的氨基酸序列组成，命名为nanT1，基因的核苷酸序列选自于序列1中2052-682碱基。
本发明还提供了一个编码ABC转移因子蛋白的核苷酸序列，由序列3中的氨基酸序列组成，命名为nanT2，基因的核苷酸序列选自于序列1中2064-2879碱基。
本发明还提供了一个编码双组分反馈调节因子蛋白的核苷酸序列，由序列4中的氨基酸序列组成，命名为nanT3，基因的核苷酸序列选自于序列1中3117-3818碱基。
本发明还提供了一个编码化学受体蛋白的核苷酸序列，由序列5中的氨基酸序列组成，命名为nanT4，基因的核苷酸序列选自于序列1中4923-3724碱基。
本发明还提供了一个编码双组分传感器组氨酸激酶蛋白的核苷酸序列，由序列6中的氨基酸序列组成，命名为nanT5，基因的核苷酸序列选自于序列1中6106-4916碱基。
本发明还提供了一个编码调节蛋白的核苷酸序列，由序列7中的氨基酸序列组成，命名为nanR1，基因的核苷酸序列选自于序列1中7493-6768碱基。
本发明还提供了一个编码调节蛋白的核苷酸序列，由序列8中的氨基酸序列组成，命名为nanR2，基因的核苷酸序列选自于序列1中8334-7573碱基。
本发明还提供了编码包括KSQ，AT-L，ACP-L，KS1，AT1，DH1，KR1，ACP1聚酮合酶结构域的核苷酸序列，这些结构域由序列9中的氨基酸序列组成，命名为nanA1，基因的核苷酸序列选自于序列1中的8919-17627碱基。
本发明还提供了编码包括KS2，AT2，DH2，ER2，KR2，ACP2聚酮合酶结构域的核苷酸序列，这些结构域由序列10中的氨基酸序列组成，命名为nanA2，基因的核苷酸序列选自于序列1中的17642-24313碱基。
本发明还提供了编码包括KS3，AT3，DH3，KR3，ACP3，KS4，AT4，DH4，ER4，KR4，ACP4聚酮合酶结构域的核苷酸序列，这些结构域由序列11中的氨基酸序列组成，命名为nanA3，基因的核苷酸序列选自于序列1中的24310-36408碱基。
本发明还提供了编码包括KS5，AT5，DH5，KR5，ACP5，KS6，AT6，DH6，ER6，KR6，ACP6聚酮合酶结构域的核苷酸序列，这些结构域由序列12中的氨基酸序列组成，命名为nanA4，基因的核苷酸序列选自于序列1中的36249-48299碱基。
本发明还提供了编码包括KS7，AT7，DH7，KR7，ACP7，KS8，AT8，DH8，ER8，KR8，ACP8聚酮合酶结构域的核苷酸序列，这些结构域由序列13中的氨基酸序列组成，命名为nanA5，基因的核苷酸序列选自于序列1中的48345-60284碱基。
本发明还提供了编码包括KS9，AT9，KR9，ACP9聚酮合酶结构域的核苷酸序列，这些结构域由序列14中的氨基酸序列组成，命名为nanA6，基因的核苷酸序列选自于序列1中的60305-65302碱基。
本发明还提供了一个编码糖基转移酶蛋白的核苷酸序列，由序列15中的氨基酸序列组成，命名为nanG5，基因的核苷酸序列选自于序列1中66747-65365碱基。
本发明还提供了一个编码甲基转移酶蛋白的核苷酸序列，由序列16中的氨基酸序列组成，命名为nanM，基因的核苷酸序列选自于序列1中66881-67798碱基。
本发明还提供了一个编码NDP-D-葡萄糖-4，6-脱水酶，NDP-D-葡萄糖-4-异构酶，NDP-D-葡萄糖-4-还原酶蛋白的核苷酸序列，由序列17中的氨基酸序列组成，命名为nanG4，基因的核苷酸序列选自于序列1中67823-68863碱基。
本发明还提供了一个编码NDP-D-葡萄糖-3，4-脱水酶蛋白的核苷酸序列，由序列18中的氨基酸序列组成，命名为nanG3，基因的核苷酸序列选自于序列1中68860-70164碱基。
本发明还提供了一个编码dTDP-D-葡萄糖-4，6-脱水酶蛋白的核苷酸序列，由序列19中的氨基酸序列组成，命名为nanG2，基因的核苷酸序列选自于序列1中71140-70145碱基。
本发明还提供了一个编码葡萄糖-1-磷酸TTP胸苷基转移酶蛋白的核苷酸序列，由序列20中的氨基酸序列组成，命名为nanG1，基因的核苷酸序列选自于序列1中72045-71137碱基。
本发明还提供了编码包括KS10，AT10，KR10，ACP10聚酮合酶结构域的核苷酸序列，这些结构域由序列21中的氨基酸序列组成，命名为nanA7，基因的核苷酸序列选自于序列1中的76990-72050碱基。
本发明还提供了一个编码环氧化物水解酶蛋白的核苷酸序列，由序列22中的氨基酸序列组成，命名为nanE，基因的核苷酸序列选自于序列1中77864-76992碱基。
本发明还提供了编码ACP聚酮合酶结构域的核苷酸序列，这个结构域由序列23中的氨基酸序列组成，命名为nanA10，基因的核苷酸序列选自于序列1中的78263-77949碱基。
本发明还提供了一个编码环氧化物酶蛋白的核苷酸序列，由序列24中的氨基酸序列组成，命名为nanO，基因的核苷酸序列选自于序列1中79763-78327碱基。
本发明还提供了一个编码酮甾异构酶蛋白的核苷酸序列，由序列25中的氨基酸序列组成，命名为nanI，基因的核苷酸序列选自于序列1中80769-79828碱基。
本发明还提供了编码包括KS13，AT13聚酮合酶结构域的核苷酸序列，这些结构域由序列26中的氨基酸序列组成，命名为nanA9，基因的核苷酸序列选自于序列1中的83192-80784碱基。
本发明还提供了编码包括KS14，AT14，DH14，ER14，KR14，ACP14，CR聚酮合酶结构域的核苷酸序列，这些结构域由序列27中的氨基酸序列组成，命名为nanA11，基因的核苷酸序列选自于序列1中的89759-83196碱基。
本发明还提供了编码包括KS11，AT11，KR11，ACP11，KS12，AT12，DH12，KR12，ACP12聚酮合酶结构域的核苷酸序列，这些结构域由序列28中的氨基酸序列组成，命名为nanA8，基因的核苷酸序列选自于序列1中的100138-89771碱基。
本发明还提供了一个编码细胞色素P450蛋白的核苷酸序列，由序列29中的氨基酸序列组成，命名为nanP，基因的核苷酸序列选自于序列1中101467-100196碱基。
本发明还提供了一个编码转录调节因子蛋白的核苷酸序列，由序列30中的氨基酸序列组成，命名为nanR3，基因的核苷酸序列选自于序列1中101593-102600碱基。
本发明还提供了一个编码转录调节因子蛋白的核苷酸序列，由序列31中的氨基酸序列组成，命名为nanR4，基因的核苷酸序列选自于序列1中102600-103541碱基。
本发明还提供了以至少来自于序列1聚酮合酶序列中的一个片段与来自于其它聚酮合酶基因簇的序列来构建重组载体以获得新型聚酮合酶的途径。
本发明还提供了在基因工程微生物体中提高南昌霉素产量的途径。
本发明还提供了得到至少包含部分序列1中DNA序列的重组DNA载体的途径。
本发明还提供了产生南昌霉素生物合成基因被打断或加倍的微生物体的途径，至少其中之一的基因包含有序列1中的核苷酸序列。
序列1的互补序列可依据DNA碱基互补原则随时得到。序列1的核苷酸序列或部分核苷酸序列可以通过聚合酶链式反应(PCR)或用合适的限制性内切酶酶切相应的DNA或使用其它合适的技术得到。通过本发明所提供的核苷酸序列或部分核苷酸序列，可利用聚合酶链式反应(PCR)的方法或包含本发明序列的DNA作为探针进行Southern杂交的方法，从其它生物体得到与南昌霉素生物合成基因相似的基因。
包含本发明所提供核苷酸序列或至少部分序列的克隆基因或DNA片段可以通过打断南昌霉素生物合成的一个或几个步骤而得到新的南昌霉素衍生物。包含DNA片段或基因可以用来提高南昌霉素或其衍生物的产量。
包含本发明所提供核苷酸序列或至少部分序列的克隆DNA可用来从南昌链霉菌基因组文库中定位更多的文库质粒。这些文库质粒至少包含有本发明中的部分序列，也包含有南昌链霉菌基因组中以前邻近区域未克隆的DNA。
本发明所提供的核苷酸序列可以被修饰或突变。这些途径包括插入或置换，聚合酶链式反应，错误介导聚合酶链式反应，位点特异性突变，不同序列的重新连接，或通过紫外线或化学试剂。
本发明所提供的核苷酸序列可以通过序列的不同部分或其它来源的同源序列进行直接进化(DNA shuffling)。
通过缺失或失活来自于相同或不同聚酮合酶系统的一个或多个聚酮合酶结构域，模块或基因，或增加一个或多个聚酮合酶结构域，模块或基因而产生新的聚酮化合物。
包含本发明的序列或至少部分序列的克隆基因可以通过合适的表达系统在外源宿主中表达以得到修饰的聚酮合酶或更高的生物活性或更高的产量。这些外源宿主包括链霉菌，大肠杆菌，芽孢杆菌，酵母，植物和动物等。
包含本发明的核苷酸序列或至少部分序列的片段或结构域或模块或基因可以用来构建聚酮合酶库或聚酮合酶衍生库或组合库。
南昌霉素生物合成修饰基因的核苷酸序列提供了通过缺失或改造这些修饰基因而得到南昌霉素衍生物的途径。
含有本发明的核苷酸序列或至少部分序列的基因或基因簇可以在异源宿主中表达并通过DNA芯片技术了解它们在宿主代谢链中的功能。
包含本发明的氨基酸序列或至少部分序列的多肽可能在去除或替代某个或某些氨基酸之后仍有生物活性甚至有新的生物学活性，或者提高了产量或优化蛋白动力学特征或其它致力于得到的性质。
通过合适的技术缺失，连接本发明中的氨基酸序列可以得到新的蛋白或酶，进而产生新的聚酮或相关联的产物。
本发明所提供的氨基酸序列可以用来分离需要的蛋白质并可以用于抗体制备。
本发明所提供的氨基酸序列提供了预测聚酮合酶三维结构的可能。
本发明具有实质性特点和显著的进步，本发明所提供的基因及其蛋白质，抗体也可以用来查找和发展可用于医药，工业，农业的化合物或蛋白。


图1南昌霉素化学结构如图1，聚酮链的编号顺序是从左到右，粗线表示用PKS催化1个乙酸起始单位，4个乙酸和10个丙酸延伸单位形成的结构单位。结合在C-19上的4-O-甲基-L-rhodinose脱氧糖的编号是从1‘至6’。
图2南昌霉素生物合成基因簇的结构组成如图2，灰色箭头标记的ORF表示I型PKS基因。负责乙酸加载延伸单位的结构域标记为ATa，负责加载丙酸延伸单位的结构域标记为ATp。黑色箭头表示修饰基因、脱氧糖生物合成基因、调节基因。
图3南昌霉素聚酮生物合成模型如图3，黑色粗线表示I型PKS蛋白亚基，黑色细线表示I型PKS模块(module)。黑色箭头代表修饰基因。
图4南昌霉素脱氧糖(4-O-甲基-L-rhodinose)生物合成途径如图4，nanG1，nanG2，nanG3，nanG4，nanG5，nanM分别代表葡萄糖-1-磷酸TTP胸苷基转移酶，dTDP-D-葡萄糖-4，6-脱水酶，NDP-D-葡萄糖-3，4-脱水酶，NDP-D-葡萄糖-4，6-脱水酶、NDP-D-葡萄糖-4-异构酶、NDP-D-葡萄糖-4-还原酶，糖基转移酶，甲基转移酶。
具体实施例方式
以下结合图1、图2、图3和图4对本发明进一步详细描述本发明中的整个南昌霉素生物合成基因簇共30个基因，具体为(1)聚酮合酶基因，即nanA1，nanA2，nanA3，nanA4，nanA5，nanA6，nanA7，nanA8，nanA9，nanA10，nanA11共11个基因；(2)南昌霉素的修饰基因，即nanE，nanI，nanO，nanP共4个基因；(3)南昌霉素脱氧糖的生物合成基因，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM共6个基因；(4)南昌霉素的调节基因，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5共9个基因。
聚酮合酶基因以下是编码催化南昌链霉菌NS3226中聚醚类离子载体抗生素南昌霉素聚酮糖苷配基生物合成所需的11个I型聚酮合酶开放读码框，即nanA1，nanA2，nanA3，nanA4，nanA5，nanA6，nanA7，nanA8，nanA9，nanA10，nanA11的核苷酸序列或互补序列及其相应的氨基酸序列和11个开放读码框所包含的I型聚酮合酶模块或结构域，即酮基合成酶结构域(KS)、酰基转移酶结构域(AT)、酮基还原酶结构域(KR)、脱水酶结构域(DH)、烯酰基还原酶结构域(ER)、酰基载体蛋白结构域(ACP)、链释放结构域(CR)的核苷酸序列或互补序列及其相应的氨基酸序列。
序列1中有11个基因(nanA1-nanA11)是用于编码聚酮合酶，其中共包含14个模块，共有74个结构域，负责催化南昌霉素聚酮糖苷配基的生物合成(如图2，图3)。
nanA1包含两个模块，即加载模块和模块1。加载模块含三个结构域KSQ，AT-L，ACP-L，负责聚酮链的起始合成，催化引入一个乙酸作为合成起始单位并最终形成南昌霉素C29-C30碳链骨架。模块1含五个结构域KS1，AT1，DH1，KR1，ACP1，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C27-C28碳链骨架，并在C28上带有一个甲基支链。各结构域的氨基酸位置如表1所示。
表1 聚酮合酶基因nanA1所包含的结构域及其氨基酸位置模块结构域 在序列9中氨基酸位置KSQ21-447加载模块AT-L 557-844ACP-L 959-1024KS11047-1478AT11585-1891模块1 DH11954-2148KR12491-2658ACP1 2768-2835nanA2包含一个模块，即模块2。模块2含六个结构域KS2，AT2，DH2，ER2，KR2，ACP2，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C25-C26碳链骨架，并在C26上带有一个甲基支链。各结构域的氨基酸位置如表2所示。
表2 聚酮合酶基因nanA2所包含的结构域及其氨基酸位置模块 结构域 在序列10中氨基酸位置KS2 34-460AT2 575-878模块2 DH2 933-1121ER2 1445-1752KR2 1761-1933ACP22055-2122nanA3包含两个模块，即模块3和模块4。模块3含五个结构域KS3，AT3，DH3，KR3，ACP3，负责催化引入一个乙酸延伸单位最终形成南昌霉素的C23-C24碳链骨架。模块4含六个结构域KS4，AT4，DH4，ER4，KR4，ACP4，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C21-C22碳链骨架，并在C22上带有一个甲基支链。各结构域的氨基酸位置如表3所示。
表3 聚酮合酶基因nanA3所包含的结构域及其氨基酸位置模块结构域在序列11中氨基酸位置KS3 42-466AT3 575-887模块3DH3 953-1158KR3 1495-1659ACP3 1775-1842KS4 1873-2301AT4 2405-2711DH4 2762-2949模块4ER4 3289-3582KR4 3591-3797ACP4 3877-3942nanA4包含两个模块，即模块5和模块6。模块3含五个结构域KS5，AT5，DH5，KR5，ACP5，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C19-C20碳链骨架，并在C20上带有一个甲基支链。模块6含六个结构域KS6，AT6，DH6，ER6，KR6，ACP6，负责催化引入一个乙酸延伸单位最终形成南昌霉素的C17-C18碳链骨架。各结构域的氨基酸位置如表4所示。
表4 聚酮合酶基因nanA4所包含的结构域及其氨基酸位置模块 结构域 在序列12中氨基酸位置KS5 35-460AT5 596-902模块5DH5 954-1144KR5 1474-1632ACP5 1732-1797KS6 1822-2249AT6 2359-2660DH6 2715-2899
模块6ER63196-3505KR63514-3666ACP6 3802-3867nanA5包含两个模块，即模块7和模块8。模块7含五个结构域KS7，AT7，DH7，KR7，ACP7，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C15-C16碳链骨架，并在C16上带有一个甲基支链。模块8含六个结构域KS8，AT8，DH8，ER8，KR8，ACP8，负责催化引入一个乙酸延伸单位最终形成南昌霉素的C13-C14碳链骨架。各结构域的氨基酸位置如表5所示。
表5 聚酮合酶基因nanA5所包含的结构域及其氨基酸位置模块 结构域 在序列13中氨基酸位置KS7 34-458AT7 583-889模块7 DH7 943-1135KR7 1449-1632ACP7 1729-1796KS8 1820-2247AT8 2353-2655DH8 2713-2898模块8 ER8 3256-3537KR8 3542-3725ACP8 3827-3894nanA6包含一个模块，即模块9。模块9含四个结构域KS9，AT9，KR9，ACP9，负责催化引入一个乙酸延伸单位最终形成南昌霉素的C11-C12碳链骨架。各结构域的氨基酸位置如表6所示。
表6 聚酮合酶基因nanA6所包含的结构域及其氨基酸位置模块结构域 在序列14中氨基酸位置KS9 35-461AT9 564-870
模块9 KR9 1209-1367ACP91514-1579nanA7包含一个模块，即模块10。模块10含四个结构域KS10，AT10，KR10，ACP10，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C9-C10碳链骨架，并在C10上带有一个甲基支链。各结构域的氨基酸位置如表7所示。
表7 聚酮合酶基因nanA7所包含的结构域及其氨基酸位置模块 结构域 在序列21中氨基酸位置KS10 35-461AT10 566-873模块10 KR10 1215-1372ACP10 1499-1564nanA8包含两个模块，即模块11和模块12。模块11含四个结构域KS11，AT11，KR11，ACP11，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C7-C8碳链骨架，并在C8上带有一个甲基支链。模块12含五个结构域KS12，AT12，DH12，KR12，ACP12，负责催化引入一个丙酸延伸单位最终形成南昌霉素的C5-C6碳链骨架，并在C6上带有一个甲基支链。各结构域的氨基酸位置如表8所示。
表8 聚酮合酶基因nanA8所包含的结构域及其氨基酸位置模块 结构域 在序列28中氨基酸位置KS11 48-474AT11 579-880模块11 KR11 1223-1401ACP11 1506-1571KS12 1598-2025AT12 2132-2433模块12 DH12 2499-2685KR12 3014-3197
ACP12 3304-3371nanA9包含一个模块，即模块13。模块13含两个结构域KS13和AT13，与nanA10的ACP结构域一起负责催化引入一个丙酸延伸单位最终形成南昌霉素的C3-C4碳链骨架，并在C4上带有一个甲基支链。各结构域的氨基酸位置如表9所示。
表9 聚酮合酶基因nanA9所包含的结构域及其氨基酸位置模块 结构域在序列26中氨基酸位置模块13 KS1343-469AT13578-685nanA10包含一个结构域ACP，它的氨基酸位置如表10所示。
表10 聚酮合酶基因nanA10所包含的结构域及其氨基酸位置结构域 在序列23中氨基酸位置ACP 17-97nanA11包含一个模块，即模块14。模块14含七个结构域KS14，AT14，DH14，ER14，KR14，ACP14，CR，前六个结构域负责催化引入一个丙酸延伸单位最终形成南昌霉素的C1-C2碳链骨架，并在C2上带有一个甲基支链。最后一个靠近C末端的CR结构域，在南昌霉素聚酮链合成的最后负责催化聚酮链从聚酮合酶上释放下来。各结构域的氨基酸位置如表11所示。
表11 聚酮合酶基因nanA11所包含的结构域及其氨基酸位置模块结构域 在序列27中氨基酸位置KS14 76-499AT14 599-902
DH14 954-1137模块14 ER14 1484-1763KR14 1770-1953ACP142048-2115CR 2146-2225南昌霉素的修饰基因以下是编码参与南昌霉素聚酮链修饰的4个开放读码框，即nanE，nanI，nanO，nanP的核苷酸序列或互补序列及其相应的氨基酸序列。
序列1有中存在四个负责南昌霉素修饰的基因，即nanO，nanE，nanI和nanP。nanO和nanE(如图2)，分别编环氧化物酶和环氧化物水解酶，在南昌霉素霉素的生物合成中，分别催化双烯中间体的环氧化和双环氧化物的开环。NanI催化激活的双键由E构型向Z构型的转化。NanO负责催化C-30位点上甲基基团的氧化。它们的核苷酸、氨基酸位置及其功能如表12所示。
表12 南昌霉素的修饰基因的核苷酸、氨基酸位置及其功能基因序列1中碱基的位置 相应的氨基酸序列功能nan E 77864-76992 序列22 环氧化物水解酶nan I 80769-79828 序列25 酮甾异构酶nan O 79763-78327 序列24 环氧化物酶nan P 101467-100196 序列29 细胞色素 P450南昌霉素脱氧糖(4-O-甲基-L-rhodinose)的生物合成基因以下是编码参与南昌霉素生物合成中脱氧糖(4-O-甲基-L-rhodinose)的合成及转移的6个开放读码框，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM的核苷酸序列或互补序列及其相应的氨基酸序列。
序列1中存在六个催化脱氧糖(4-O-甲基-L-rhodinose)的生物合成和转移的基因，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM(如图2)。从D-葡萄糖-1-磷酸开始，通过NanG1(葡萄糖-1-磷酸TTP胸苷基转移酶)的作用将其转变成dTDP-D-葡萄糖，随后又在dTDP-D-葡萄糖-4，6-脱水酶(NanG2)催化下转变成dTDP-4-酮基-6-脱氧-D-葡萄糖，dTDP-4-酮基-6-脱氧-D-葡萄糖再经NanG3的催化转变成dTDP-4-酮基-2.6-双脱氧-D-葡萄糖，接着产生dTDP-D-cinerulose，dTDP-L-cinerulose和dTDP-L-rhodinose的等一连串反应，这些反应均被多功能酶NanG4催化。最后，NanM将完成L-rhodinose的4-O-甲基化并在糖基转移酶(NanG5)作用下将4-O-甲基-L-rhodinose连接于糖苷配基而形成成熟的南昌霉素。整个南昌霉素脱氧糖(4-O-甲基-L-rhodinose)生物合成途径见图4。它们的核苷酸、氨基酸位置及其功能如表13所示。
表13 南昌霉素脱氧糖生物合成基因的核苷酸、氨基酸位置及其功能基因 序列1中碱基的 相应的氨基酸序 功能位置 列nan G172045-71137序列20 葡萄糖-1-磷酸TTP胸苷基转移酶nan G271140-70145序列19 dTDP-D-葡萄糖-4，6-脱水酶nan G368860-70164序列18 NDP-D-葡萄糖-3，4-脱水酶nan G467823-68863序列17 NDP-D-葡萄糖-4，6-脱水酶，NDP-D-葡萄糖-4-异构酶，NDP-D-葡萄糖-4-还原酶nan G566747-65365序列15 糖基转移酶nan M 66881-67798序列16 甲基转移酶南昌霉素的调节基因以下是编码参与南昌霉素生物合成调节的9个开放读码框，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5的核苷酸序列或互补序列及其相应的氨基酸序列。
序列1中存在九个调节基因，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5(如图2)，它们参与南昌霉素生物合成的调节。它们的核苷酸、氨基酸位置及其功能如表14所示。
表14 南昌霉素的调节基因的核苷酸、氨基酸位置及其功能基因 序列1中碱基的位置 相应的氨基酸 功能序列
nan R17493～6768 序列7 调节蛋白nan R28334～7573 序列8 调节蛋白nan R3101593～102600 序列30 转录调节因子nan R4102600～103541 序列31 转录调节因子nan T12052～682 序列2 膜整合型转移蛋白nan T22064～2879 序列3 ABC转移因子nan T33117～3818 序列4 双组分反馈调节因子nan T44923～3724 序列5 化学受体nan T56106～4916 序列6 双组分传感器组氨酸激酶序列1为南昌霉素生物合成基因簇共30个基因的核苷酸序列或互补序列，全长132554个碱基，包含11个用于编码聚酮合酶的基因(nanA1-nanA11)，4个参与南昌霉素生物合成修饰的基因(nanE，nanI，nanO，nanP)，6个参与脱氧糖(4-O-甲基-L-rhodinose)的合成及转移的基因(nanG1，nanG2，nanG3，nanG4，nanG5，nanM)和9个调节基因(nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5)。南昌霉素生物合成基因簇的结构组成见。
序列2为nanT1基因(序列1中2052-682碱基)编码的膜整合型转移蛋白(NanT1)的氨基酸序列。
序列3为nanT2基因(序列1中2064-2879碱基)编码的ABC转移因子(NanT2)的氨基酸序列。
序列4为nanT3基因(序列1中3117-3818碱基)编码的双组分反馈调节因子(NanT3)的氨基酸序列。
序列5为nanT4基因(序列1中4923-3724碱基)编码的化学受体蛋白(NanT4)的氨基酸序列。
序列6为nanT5基因(序列1中6106-4916碱基)编码的双组分传感器组氨酸激酶(NanT5)的氨基酸序列。
序列7为nanR1基因序列1中7493-6768碱基)编码的调节蛋白(NanR1)的氨基酸序列。
序列8为nanR2基因(序列1中8334-7573碱基)编码的调节蛋白(NanR2)的氨基酸序列。
序列9为nanA1基因(序列1中8919-17627碱基)编码的I型聚酮合酶(NanA1)的氨基酸序列。
序列10为nanA2基因(序列1中17642-24313碱基)编码的I型聚酮合酶(NanA2)的氨基酸序列。
序列11为nanA3基因(序列1中24310-36408碱基)编码的I型聚酮合酶(NanA3)的氨基酸序列。
序列12为nanA4基因(序列1中36249-48299碱基)编码的I型聚酮合酶(NanA4)的氨基酸序列。
序列13为nanA5基因(序列1中48345-60284碱基)编码的I型聚酮合酶(NanA5)的氨基酸序列。
序列14为nanA6基因(序列1中60305-65302碱基)编码的I型聚酮合酶(NanA6)的氨基酸序列。
序列15为nanG5基因(序列1中66747-65365碱基)编码的糖基转移酶(NanG5)的氨基酸序列。
序列16为nanM基因(序列1中66881-67798碱基)编码的甲基转移酶(NanM)的氨基酸序列。
序列17为nanG4基因(序列1中67823-68863碱基)编码的NDP-D-葡萄糖-4，6-脱水酶，NDP-D-葡萄糖-4-异构酶，NDP-D-葡萄糖-4-还原酶(NanG4)的氨基酸序列。
序列18为nanG3基因(序列1中68860-70164碱基)编码的NDP-D-葡萄糖-3，4-脱水酶(NanG3)的氨基酸序列。
序列19为nanG2基因(序列1中71140-70145碱基)编码的dTDP-D-葡萄糖-4，6-脱水酶(NanG2)的氨基酸序列。
序列20为nanG1基因(序列1中72045-71137碱基)编码的葡萄糖-1-磷酸TTP胸苷基转移酶(NanG1)的氨基酸序列。
序列21为nanA7基因(序列1中76990-72050碱基)编码的I型聚酮合酶(NanA7)的氨基酸序列。
序列22为nanE基因(序列1中77864-76992碱基)编码的环氧化物水解酶(NanE)的氨基酸序列。
序列23为nanA10基因(序列1中78263-77949碱基)编码的酰基载体蛋白(NanA10)的氨基酸序列。
序列24为nanO基因(序列1中79763-78327碱基)编码的环氧化物酶(NanO)的氨基酸序列。
序列25为nanI基因(序列1中80769-79828碱基)编码的酮甾异构酶(NanI)的氨基酸序列。
序列26为nanA9基因(序列1中83192-80784碱基)编码的I型聚酮合酶(NanA9)的氨基酸序列。
序列27为nanA11基因(序列1中89759-83196碱基)编码的I型聚酮合酶(NanA11)的氨基酸序列。
序列28为nanA8基因(序列1中100138-89771碱基)编码的I型聚酮合酶(NanA8)的氨基酸序列。
序列29为nanP基因(序列1中101467-100196碱基)编码的细胞色素P450(NanP)的氨基酸序列。
序列30为nanR3基因(序列1中101593-102600碱基)编码的转录调节因子(NanR3)的氨基酸序列。
序列31为nanR4基因(序列1中102600-103541碱基)编码的转录调节因子(NanR4)的氨基酸序列。
以下进一步提供应用实例，这些实例只是阐明了得到和应用本发明所提供序列和要素的优选的途径，仅用做说明而并不限制本发明的应用范围。
应用实例1缺失南昌霉素生物合成基因簇中所有基因缺失序列1中从nanT1序列中的一个BamHI位点(1035bp)到南昌霉素生物合成基因簇之外的另一个BamHI位点(125077bp)之间124042个碱基。它包括了整个南昌霉素生物合成基因簇。用于同源交换的两个大小分别为8.4kb和4.3kb天然同向片段分别来自于科斯质粒2G2和2F7，在这两个片段中间插入一个阿泊拉霉素抗性基因构建成基因置换载体pHZ1586。通过结合转移将pHZ1586导入南昌链霉菌NS3226并筛选得到基因置换菌株SYH-8，通过高压脉冲电泳和分子杂交验证了基因置换菌株的正确性，经过生物学活性和高效液相色谱分析证明了所得到的基因置换菌株中南昌霉素生物合成被阻断。此基因置换证明分离到的序列(序列1)确实是负责南昌霉素的生物合成。
这提供了一个通过完全敲除南昌链霉菌中南昌霉素的生物合成基因簇，以减少底物和能量竞争达到提高同类型其它抗生素产量的途径。还提供了一种获得染色体上大片段DNA敲除的技术手段。
应用实例2缺失南昌霉素部分聚酮合酶基因缺失序列1中从nanA3序列中的一个BamHI位点(30332bp)到nanA5序列中的另一个BamHI位点(57743bp)之间27411个碱基。它包括了聚酮合酶基因nanA3中的部分KS4，AT4，DH4，ER4，KR4和ACP4结构域；包括了聚酮合酶基因nanA4；包括了聚酮合酶基因nanA5中的KS7，AT7，DH7，KR7，KS8，AT8，DH8结构域。用于同源交换的两个大小分别为4.8kb和5.8kb天然同向片段来自于科斯质粒11A8，在这两个片段中间插入一个阿泊拉霉素抗性基因构建成基因置换载体pHZ1553。通过结合转移将pHZ1553导入南昌链霉菌NS3226并筛选得到基因置换菌株SYH-5，通过分子杂交验证了基因置换菌株的正确性，经过生物学活性和高效液相色谱分析证明了所得到的基因置换菌株中南昌霉素生物合成被阻断。此基因置换证明这些序列对南昌霉素的生物合成是必需的。
这提供了一个通过置换南昌霉素生物合成基因簇中部分结构域而获得相应结构缩短了的新的南昌霉素衍生物。
应用实例3缺失南昌霉素脱氧糖生物合成基因、修饰基因和部分聚酮合酶基因缺失序列1中从nanA5序列中的一个BamHI位点(57743bp)到nanA11序列中的另一个BamHI位点(83968bp)之间26225个碱基。它包括了聚酮合酶基因nanA5中的ER8，KR8和ACP8结构域；包括了六个催化脱氧糖(4-O-甲基-L-rhodinose)的生物合成和转移的基因，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM；包括了三个负责南昌霉素修饰的基因，即nanO，nanE，nanI；包括了聚酮合酶基因nanA10；包括了聚酮合酶基因nanA11中的ACP14和CR结构域。用于同源交换的两个大小分别为4.5kb和7.3kb天然同向片段来自于科斯质粒18A2，在这两个片段中间插入一个阿泊拉霉素抗性基因构建成基因置换载体pHZ1581。通过结合转移将pHZ1581导入南昌链霉菌NS3226并筛选得到基因置换菌株SYH-1，通过分子杂交验证了基因置换菌株的正确性，经过生物学活性和高效液相色谱分析证明了所得到的基因置换菌株中南昌霉素生物合成被阻断。此基因置换证明这些序列对南昌霉素的生物合成是必需的。
这提供了一个通过改造南昌链霉菌中修饰酶基因、糖生物合成基因而得到相应结构改变的新的抗生素的途径。
以下根据本发明的内容提供基因序列序列列表SEQUENCE LISTING<110>上海交通大学江西农业大学<120>南昌霉素生物合成基因簇<160>31<170>PatentIn version 3.1<210>1<211>132544<212>DNA<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1tccggctgta cccgatgccg gtcggcgcgt ctcccggggc ggtgccgggg tcggcctggc 60cgacggcggc cagccagagg gtggcgtcca gggctcgggc ccgtaccagc agctcccact 120ggtcgagctt gccgggcccg gctccccagg aggccgccag cagtgtcgcg gccgccccgg 180cgtccgcatg tgcccggaac agctccggga agcggacgtc gtagcaggtg gccagggaca 240gccgtacgcc gtccacctcc atgacggtgg tccgcgagcc cgcggcgacg gtgtcggact 300cgcggtagcc gaaggcgtcg tagaggtgga tcttgtcgta cgacgtctcg acgccggggc 360cggtggccag cagggtgttg gtcaccttgc cgccgtccgc cggggtgaac atgccggcca 420cgatgacggt gtcggtggcc ctggcgatct cccgtactcc gtcggcccac ggtccgttca 480ggggttcggc gagcgctgcc agtggggtgc cgaagcaggc catcgaggcc tcggggaaga 540cgacgacacg ggctccggcg tccgcggcgc gctgtgccca ttcctcgatg agccgcaggt 600tcttcccggg gtccgggccg gtggtgagct ggctcagggc gatccgcata tttcctcctc 660agtgggtcgg actgcagtgg gtcagacggc agtgggtcgg gcggcgtcgg ccgtggcctg 720cgcggccggt gtggcgagct gggcgtcctc gtcgacggcg gcttcgggat cgcggcccag 780gagcaccccc gcgagggaga cggccgcggc gacggcgatg tagagggcca gcggcagcca 840gctgccgtag gcgctcagca gggtggtgaa cagcagcggg gcgatggcgc cgccgatgat 900cccggccagg gtgtacgcca gcgatgagcc ggtgtagcgc agccggggcg agaactgctc 960ggcgatgaag gcggcctgcg gcccgtacag gaaggagtgg atcacgaggc ccaccacgac1020gccgagcgcc agcgggatcc agctgccgcc gccgatcatc gggaagaaca ggaacggcca1080cacaccggcg gccaccgcgg cacagccgta cagcactcga cggttgatcc ggtcggagac1140ggcaccggcc agcggcatca ggaagacctg cagcgaggag ccgatgagga cggccgcgag1200ggcggaaccg cgcgacatgc ccagttctcc ggtggcgtac gtcaggacga agacggtgaa1260catggcgtac agcacgtcgg ggccgacgcg gcagaggatc gcggcgagca gggcgcgggg1320ctgggtggtg aagacctcgc ggatcggcgc ctcgggccgg gactgctcgg cctccatcgc1380cttgaagacc ggggtctcct ccagcttcgc cctgatccac aggccgaagg cgacgagggc1440gccggagagc aggaaggcca cgcgccagcc ccatgcctcg aactgcgcct cggtcagcag1500cgcgccgagg gcggccagga cgccgttggc caggaggttg cccgcgggcg ggcccacctg1560cgcggcggag gcgtagaaac cgcggcggcg cgagtcgccg aactcgctgg acagcagcac1620cgcgccgccc cactcgccgc cgacgcccac gccctgcgcg aaccgcagca cgaccagggc1680gatcggggcg gcgaccccga tcgtggagta cgtgggcagg acgccgatca ggaacgtggc1740cgcgccgatc aggaccagtg tggcgatcag caccttcttg cggccgatca catcgccgag1800gcgaccgaag acgatcccgc ccagcgggcg cgagacgtag ccgaccgcgt atgtggagaa1860ggccagcagc gtgccggtga gcgggtcctc cgaagggaag aagagatcgc cgaagaccag1920ggccgccgcg gcggagtaga cggcgaagtc gtaccactcc agggctgtgc cggtcaggct1980cgcgacaaag gcccgccgga cgccggagcg cctctcggac gccctcggat cgtccttggt2040ggtgtcgtgc atggttccca tccttgctcg ggggacatcg accttccggc atacttcttg2100tatacaggtc gtatgcgcaa gccctgcgca agaagttaac cgcggagaaa tccaccaccg2160cggaccgtca cccgaactct gggagtgccc ccatggcccg cctgaccttc gaactgccgg2220acggatcgac acgcgaggtc gacatcgtcc aggtcctcaa cgccgggtac gccgggcgca2280gccaggacga cgtcgccgca cacatcgccg aactcgccga gctcggcgta cccaccccct2340ccgtgacccc ggccctctac cccgtcgccc cctacctcgc ccagcagatc gaccgcgtcg2400cggtccagca ccggcgcacc tccggcgagg ccgagtgggc gctcgtcgtg gcgggcgacg2460gcgagctgct gctcacggcc gcctgcgacc acaccgaccg cgacctcgag gtgcacggcg2520tggcctggag caagaacgcc ggccccgacg tcctcgcccg ccgtgcctgg cggctcgccg2580acgtcgaacc acgtctggac gacctcaccc tgcgcgcctg ggtcacccgc gacggcaccg2640agaccgagat ccagcacggc accctcgccg aactcctcac ccccgcctac tgggtcgacg2700tcctgcgctc ccgcgacgcc ctcaccccag gcaccgtcct catctccggc accatcccca2760tgaccccggg tgtcgaccag ttcgccgaca cctggcgcgt ggaactgggc gaccccgcca2820ccggcgacac catccgcctc gcctacgacg tgcaccccat gccggaaccc atcggctgac2880gagccgctgc ggccgccgcg cgaagctgcg gccgcaggac cacacacgac ccggacatat2940gttcactttg tcgaacatgc gttcattaac ggaatctttc gccgcacttc cgcatccgac3000gtgtgaaacc cggggagtct ttgagcagga tcggctccga ttgcacggga accccaagca3060tgacgtgaag catcccagca agcaggtcca ttccccaaaa ctcggggcac gtcagtttgt3120ctactcgttg tacaggttct ctcacgaatg ggtgggtgag tgtggagtta ggaattcccc3180tcagcgtcgt gttggtcgat gatcaccccg tggtgcgcgc gggcatctcg gcgtggtgtg3240ccgccgccga cgcaccgatc agcgtggtcg ccgagggggc gaacgtgtcg gtggccctcc3300atggtccggg ccggggggcg gatgtcgtgg tcatggatct gttgttgcag aacgggcggc3360ccgcgtacga cgagttgcag gagctcgtgg cccaggagcg caaggtcgtc gtctacacca3420tgcgggacag ccaggatgcg gcgttgacct gcatggatct gggctcggct acctacatca3480ccaaggcgga ggggcagcgg caccttggtcaaggcgatccg ggccgccgcc gaggacatcc3540cctatacacc cccgtccctg gccggcgcgt tcggcagcga cacccggcag agccgccccg3600tgctgtccgt acgggaggtg gaggtgctgg tggagtggtt ccagtccgag tcgaaggcgg3660tggtcgccca gagcctgggg atctcggagc gcacggtgaa cacctatctg gaccgggtgc3720gcatcaagta cgcgaacgcc ggccggccgg cgaccacgaa ggccaagctg gtggccaggg3780cggttcagga cggtctgata gctctggacg agctgtgatc cggcaggcgg cctccacgag3840gagacggtcc tcggcgtgga ccgtgcgtac ccggatcccg ttcgccttcg gctcgggcag3900ccggtcccgc agcgcgtcca ccacgacgcc cacccgcacc tgatccccgc cgcggaccac3960ggtgacgcgc gccgtctgcc cggcggcggc cagcgcggcg atgaccgggt ccaggagcgc4020gcggcgcagg ggcaggggca gttcgagggg gtcgccgcgg acggcgagtt gcacggtggt4080cccgttgcgc tcggcgacgt cgatacaggc gcgcatctcg tggaccagcg gatcgaacgt4140acagtcgctc tccgcgaaca gccgcctcag ccgggcggct tccagcgcgc aggcgtgccg4200cacctcgtcg tcctccgggt cgagggagcc gctggcgagc ccggtgagca gggggagcac4260ggtggcgttc agctcggcgt accggcggcg ctggtccgcc tggatctgcc gggcgactgc4320gatacgggtg cgtacccgct cctgggcccg cagggcctgg ccgatggccg cgctgctgtc4380gaggagcagc ttggcgccgg cggcgatggt catctggaag cagccggcgg acagggcgct4440cagggccatg ctcgccccga tggaccgcga gggcacgtcg acggtcagca acagggcggc4500ggtgacggcc agatgagcgc cgaggaaggc gccggacacc agcggtcctc ggtggccgag4560caggacgacg gtgaaccagc ccgcgagcga gtaggaccag tgcaaggagc tgacgaagta4620gtggtccgcc gggacctggc tggtggtggc caccgaggcg acgaaggtgc cggccagcgc4680gcatggcacc cagccgcgcg gccaggggcg cccgtcgagg ccgagaacca cgctgccgcc4740gaggatcagc gtgagcaggc cgtaggcggc cagggggatc caggggtgcc ggtattcgcc4800caggccgccc agcaggccgg gcagcgcgga cccgatgtgc agagtgacca ggatgaccag4860ccccccggcg cggatgccac ggagctggtg acgggtgatc agctgccgga agtcctcacc4920cacgcggcca ctccagccgc accgtcgtac cgtggccggg ccgggactcg acggtggcgc4980ggccgcccac gttccacatc cggccgtgga tcgaacgctt gatcccgatc cggtggcctg5040gaatgtgctc cgggtcgaac cccaccccgc tgtcggcgat ggtcacgacg atgacatggt5100ccacgcgctc cgccgtgacc cgtacggcat cgacgccagc gtaccgggcc acgttgcgca5160gcgcctcgcc gaggccgccg cgcagggctg ccgcgatggg gcgccaggtc gtgcccagtt5220ccacgtcgaa cctgttgatg acgcggaggg gatgcgcggc gatctcgtcc agcagttcgg5280cggccaggtc cacctccccc gccaccggct gctcggcgcg cagccggagg agatctctgg5340cggcctgggc gcgcagcgtc tccgcccgca tcgacgccca tggtgccgac gcgatcagca5400gggtcgcgca ggcggtgtcg tggagcgcgg ccaggtgttc ccgttccgcg gcgcagcggg5460cctccgccgc ctccccctgc tgccgcgccc gcgcggcccg gtcggcggcc tcgtcggccg5520cccggctctc gctgaacacc agccggacca cggcccgggc cagggcggtg ttcaccacca5580cccagatgac actgcgcagc acacctgacc actcgaggtt cgggtgcaac gcggtgccca5640ccacatccgc gcagcacagc cataacgtgg ccagaatccc ggtgagcggc ggctgcatca5700gctggtacgc cacggccgtg aagctgacgg acaccaggat ccaggtgccg gcatgcgtgg5760tctgcgcctc ggggatcgtg aaccgctggc tcagacacag cgccgtcatg accgtcagat5820ccagggcgag cagcggcctc tgccgcatcg ccgaggtggt ggcgagccgc agatggatcc5880agctccaggc gagtacggcc gccgcgacca ccgccgcggg cgccatctcg cccatcggca5940tggccgccac gcccagcacg gcgcaggcgc tcatcacccc ggcgcggccg tagagcgcca6000accgcctgtt gaacgcgacg tattcgcgcg cccaggggcc ggccgagccg ggaaggcgcg6060ccgaggccgg cggatcgata ccgggcgaca gtgagctaat cgacatgggt catccatcct6120cgacggccgt cggtgtgacg gggcgatagc ggaatcctca cgatccgtga gggaattgat6180gcctttttgt cggtcgaacg accctcacac cggatgaccc ggcgcctcta ggctggaccg6240agcgcagtgg cttaggggga gcagatcagg acggtctgga agcccaccca tgtcgggccc6300ccacaagccc tttgtgagca agaagtatgt cgcccatcgg tgcgctttcc gcctcatacc6360aaccttctct tgggtgtata gcgaagggaa tgcagccttt tatgtccgca tctgcgccca6420acgtcatcct gcgcggcggc cccacggagc tggccgatcc gcacacgcgg cacgtcgatg6480acaccgagtc gaccctgaag cttctcatcg gcaacgcgta cgaacacttc gaacccacag6540gccagttcgt ggagcaccac ggacagcatc tgcgggtctt cgagtggatc cgccggacct6600acgtcgccga gtaatcagcc aagtcagcca agtggccaac gggtcgattg gccaacaagc6660ggccaaagcc cgcaagggcc cggaacatcg ccggtgggtt cactcgcggc atgcgaggaa6720acccccggcg agctcgggcc caggtggcgt ccgcctggtg gattccgtca ggtcgaggcg6780agcaccgtgt gccggagctc cctgagcttc cggccgggtt cgagccccag ttccctgtcg6840agaacacgcc gggcggactc gtatatgcca agcgcctctg acttctggtc ggagcggtag6900agggccagca tcagctgccg atagaacgtc tcccggagcg ggaactcggc gaccagggaa6960cgtagtcggc cgacgagttc ccggtggcgg ccgagttcaa gatgcgcctc ggccgacatt7020tccgcacatt cgatgcgcat ctcgtccagc cgcttcagaa agccggccac gatcggcccg7080gtctggacgt tgccgaacgc cgggccccgc cacagcgcca gggcctgctc gaaacagagg7140gccgcctgcg ccggctcccc gtatctgacg cacttccggc cgtccgcgac cagctgctgg7200aagacatcga aatcgcattc gctggacgct ctgcgcagca tgtatccacc ggggcgggtg7260atgatgggat tctgctggcc cggccggctc aggaacttgc gaagccgcga aatgcagacg7320tagatgctcg cggtgtcgcg gcgcggcggc agctcgcccc agatctctct ggtgagctgt7380tccgccatca caacctgatc cgagcggatc agcaggacgg cgagaacggc agcgagtttc7440tgcgctctga gcggtgaacc gcccttttcg tcgactacac ggagaggtcc caaaatctcg7500taccgcacga attaaccccc tgctctgcta ttcctggata cgtgacgagt cggcggtgcg7560cacgggattc ggctatgcgg cctcgagttc cggggcgtcg acgagcacgg cgtgatgcaa7620ccgcttgaga gcggcggacg gctccagccc gagttcctcg ctcagcacgg cccgcgccga7680ctggtagaca tgcagggcgt cggccctgca gcccgagcgg tagagcgcca gcatgagttg7740ccggtagaac gcttcgttca gcggaagctc ctctatccgg gcatagaggt agctgacgaa7800ttcacggtgc cggccgagtt ccagtccggc ttccacgaac gactcgacgc attccagccg7860ggcctgctcg gcccagcata cgaagccgtt gacgatcgcc ccgtcgcgca ggtcttcgag7920gaccggcccc cgccagaact caagaccctc ctcgtaggcc tcgcaggcct catgggtgcg7980cccggcccgc tgtgcggccc gcccctggcg caccaggccc tggaagacat ggaagtccag8040ctcgtcgtcg cccagtcgca gcacataccc gctgcgagcg gtgacgatgg ggctctcggt8100gcggttgggg cgcctgagga acttgcggag ttgggacaca tacacatgga gcgccgccac8160ggcctgccgc ggtgcccggg taccccagat ctccttcacg agatcttcgc tgctcaccac8220ctcgtcggcc cgcacgagta atgcggccag cagcacctcg atcttccggg cgctaattga8280ggtgaccgaa gatcggtcaa ccacgcgcaa cgggcccagt atctcgtacc tcatgatacg8340cgaactcccc ccgttgccag cgtcttgtcg aaccaggagg gcgctccatt gtgatcgaat8400tcagccagca ttcgaaaaat tcaccccgat tttggaagcc tttttggtcg cgcttcagac8460ctcagcgtcg ttgcctaccc acgccaccaa cagcttagcc agatcgcggc cgcgtccgcg8520gatttcacac ggcggcaaca ttgcctcggt gagattttcg gtcaggcggg aggtggtctt8580ctggccatgg agatcgacgg cgttgctctg cgatccagtg atcgcagaga gggcaacatc8640aacgcatcct aaccgtgcgg cgcgcgccag tgtgagggtc aaaagaccca caaccaaatg8700ggcacatatc aggattcgcc gatcagtgac cgtcaccgta ccctgctgtt tcggcgacgg8760tcccccgcag gtcaccgatc cgatccgaag gatcgtcagg gcaagtttcg cctggcgtat8820acaaggcgct ccctagacat tctcggttag atcaacttta gaaaccattc ggagaatgag8880cgctgttcgg ggcatctcat aagccataac cagcggtttt gcgattcagg tatgggggtg8940tcatggccgg gagcagcccg agccacgcgc agcaggcgac gtctcccgtc gcgatcgtgg9000ggctggcctg ccggctgccc ggcgcacccg atcccgaggc gttctggcgc ctgctgcgcg9060cgggcgagaa cgccgtcgtg cccgtcccgg acagccgcct gccgaccgag cccggttccc9120cgccgtactt cgccggactt ctggagcacg ttgacacgtt cgacgccgga ttcttcggca 9180tctcgccacg cgaggccacg gcgatggacc cgcagcaacg gctgatgctc gaactggcgt 9240gggaggccat ggaggacgcg gggctcggcc ccaagaacct ggcggagcgc cggaccgcgg 9300tgttcaccgg cgcgatctgg gacgactacg ccacgctcct ccaccggcgc cgccccaacg 9360acatcgccat cacgcggcac acgatggcgg ggctgcaccg cggcctcatc gccaatcggg 9420tctcccacct gctgggcctg cgcggaccga gcctcacggt ggacgcggcc cagtcctccg 9480ggctcgtcgc cgtgcacctg gcctgcgaga gcctgcgccg gggcgaggcc gacctggcgc 9540tcgcgggcgg ggtgaacctg atcctggccg aggagagcat gcgtatggcc gaggcccagt 9600tccagggcct ctcgccggac ggccggtgct acaccttcga cgcgcgcgcc aacggcttcg 9660tgcgcggcga gggcggcgga atggtgctgc tcaaaccgct ggccgcggcc gtcgccgacg 9720gcgacccggt gtactgcgtg atcgagggca gcgcggtcaa caacgacggc gccaccgacg 9780gcctgacccg gccgagcgcc gacgcccaga cggatgtggt gcggcaggcg tggcagcggg 9840ccgcggtgtc gcccgccgaa ctccagtacg tcgaactgca cggcaccggt acgccggtcg 9900gcgatccgat cgaagccgcc gcgctcggtg acgcgctcgg tgacgcattc gatgggtgcc 9960atcgggaccg tgcggtggac gcgccgctgc gggtcggctc ggtcaagacc aacgtgggcc10020atctggaggc cgccgccggt atcgcgggcc tgctgaagac ggccctgagc atccaccacc10080ggcgacttcc gccgagtctc aacttcgcca ccccgaaccc cggcatcccg ctggccgaac10140tcggcctgcg cgtacagacc gcgttcgggc cctggcccga cgagcggcgg cggctcacgg10200ccggggtcag ctccttcggc atgggcggta ccaactgcca tgtcgtcctg gccgaaccgc10260ccgccccggc cgtgctgccg gaccggccca cggcgcccgg ggacgtgtcc gcgcgcaccg10320ccccgccggt catgccctgg gtcgtatcgg cggcgagtcc gaaggccctc acggcccagg10380ccgccgcgct gtacgaacac ctgcgcgcac accccgggct ccacccggtg gatatcggcc10440acgcgcttgc cacgacccgt acggcgttcc cgcaccgagc cgtcgtcctc ggcagggacg10500aagacgaact ggtaagccga ctcgacgcgt tggcctccga aacacagaca tcgggagtga10560tccgtggccg cgcgggcggg ggccgggtcg cgttcctgtt cagcggccag ggcagccagc10620gccccggcat gggccgcgaa ctgtacgcgg cgtacccggt cttcgccgac gcgctgcgcg10680aggtgtgcgc gcacctggac cccatgctcg acaccgacac cccgctgctg gacctcatgt10740tcgccgaggc cccaccggac ggcgagccgc cgctgaaccg gaccgcgtac acccagcccg10800cgctcttcgc catcgaggtg gccctctacc gcctggtgac ctcgtggggc gtgacccccg10860accacctgat gggccactcg gtcggcgaga tcacggccgc gcatgtggcc ggggtgctgt10920cgctgcccga cgcctgcacg ctggtggcgg cccggggccg cctcatgcag tccatcaccg10980cccccggggc gatggccgcc tggcaggcca ccgccgagga ggccgggcag gcgctggagg11040cgtacggcgg acgggtgggc ctcgcggccg tcaacgcacc cgcctccgtg gtgatctccg11100gcgaccgcga ggccgtggcg gaggcgaccg ccgcctggcg ggcgcggggc cgcaaggcca11160ccgtgctcaa ggtcagccac gccttccact ccccgcacct cgacggcatc ctcggggacc11220tgcgcacggt cgcggccggg ctgacattcg ccgcgcccgc catccccgtc gtatccaatc11280tgaccggcgg ggcggccacc gaggcccagt tgcgctcgcc cgactactgg gccgaccacg11340cccggcaggc cgtacggttc gacgccgggg tacggcacct gtgcgacgcg ggggtcgaca11400ccttcctcga actcggcccc gacgcctcgt tgaccggcat ggcgcgggag agcgccgcgg11460catgggccgg cgacgcgccc cgcccggtgg cggtcgcggt gcagcgccgc ggccgaccgg11520aggcgcagag cttcgtgtcg gcgatggccc aggcgcacgt acggggcgtc ggcgtcgact11580gggcggccgc gttcgcgggc cacgagaccc ggcgggcgcc cctgccgacg tacgccttcc11640agcgcgatcg ccactggccc gacgggctgg acgagcgggg ggcgaggcgg ccgagtacct11700cgccggtcgt gccgtcaacc gaccgtgagc cggtcgtcgt tgacgcgtcc cccgccgatc11760gcgcggcctc cccgggcgag ctgctggcgc tggtacgtac ccacgccgcc ctcgtactcg11820gacacaacag ccccgacggc atcgacccgg ccctgacctt caaacagctc ggcttcgact11880cgctggccgc cacggagctg agcgaacgcc tgagcgcggc cacggacacc gaactccccg11940ccaccctcac cttcgaccac ccgacgccga acgccgtggc ggcgtggctg cgcgcggcgc12000acgaggggca gcccaccgcc gccccgacgg cggcgacagg gccgtccatg gccgaggacc12060ccgtcgccgt ggtggccgtc agctgccgct accccggcgg ggtcgagtcg ggcgaggcgc12120tgtggcgcct ggtggacgaa ggggtcgacg ccgtgggcga gttccccggc gaccggggct12180gggatctggc ggagctgttc ggccgggcgc cggacgggag cggcggcagc gcgacgggcc12240ggggcggctt tctgtacggc gccggggact tcgacgcgga gttcttcggg atcagcccgc12300gcgaggcgct ggccatggat ccgcagcagc gcatcctgct cgaactctcc tgggaactgc12360tggaacgggc cgggatcccc ccggcctcgc tggccggcag cgcgaccggc gtctacgtcg12420gggcgacggc ggtggactac gggccgcggc tgcacgaggc caccgccgag ctcgacggac12480acctgctgac cggctccacg ccgagcgtgg cgtccggccg ggtggcctac gccctcggcc12540tggagggtcc ggcgctcacg gtggacacgg cgtgttcgtc gtcgctggtg gcgatgcacc12600tggccgcgca ggcgctgcgg cagggcgaat gcgatctggc gctggcgggc ggtgtgacgg12660tgatggccac accgggcatg ttcacctcct tctcccggca gcgcgggctg gcgcctgacg12720ggcggtgcaa gccgttcgcc gccgccgccg acggcacggg gtggagcgag ggcgccgggc12780tcgtcctgct cgaacgcctc tccgacgccc gccgcaacgg ccaccaggtg ctcgccgtga12840tccgcggctc ggcggtcaac caggacggcg cgagcaacgg cctgtccgcc cccaacggcc12900cctcccagca acgcgtcatc cgccaggccc tggccaacgc ccgcctcgaa cccgcggacg12960tggacgcggt cgaggcccat ggcacgggca cgacgctggg cgaccccatc gaggcccagg 13020ccctcctggc cacgtacggc gggcaacgca ccgacgaccg gccgctgtgg ctgggctcca 13080tcaagtcgaa catcggccac acccaggccg ccgccggcgt cgcgggcgtc atcaagatgg 13140tgatggccct gcgccacggc cgcctcccgg ccagcctcca catcgacgcc cccagcccgc 13200atatcgactg gtccgacggt acggtcaggc tcctcagcga gcccgtcgac tggcccggga 13260ccgactggcc cggatccgac cggccccgcc gcgccgccgt ctcctccttc ggcatctccg 13320gcaccaacgc ccacctcatc ctcgaacagg cacccgacca ccccgaaccg gagcccacca 13380cctcgggcgg cgtggtgccc tgggtgctgt cggcccgtac cgccgacgcg ctccgcgccc 13440aggccggccg cctggccgag tgggtcaccg ctggcgcccc gcgctccccg gcctccccga 13500cctccccggc ctccccggcg gacgtggggt ggtcgctggc caccacgcgg tcggcggacc 13560gccaccgagc cgtggttagc ggtacggacc gggacgagct gctgtccggg ctgcgcgcgg 13620tggcggacgg cctcgcgccc gccgccgtct cggcgggggc ggcccccggc ccggtcatgg 13680tgttccccgg acaggggtcg cagtggcgtg gcatgggcgt ggaactgctg gactcctccc 13740ccgtcttcgc ggcccgcatg gccgcgtgcg aggcggcgct cggcgagttc gtcgactggt 13800ccctcaccgc cgtgctgcgc ggcgcgccgg gcgcgcccga gccgtcccgg gtcgatgtcc 13860tccagccgtg cctgtgggcg gtgatggtgt cgctcgccgc cgtctgggag agctacggcg 13920tgacgcccac cgcggtcgtc ggacactcgc agggcgagat cgccgccgcg tgcgtcgccg 13980ggggcctgtc gctccgggac ggcgccaggg tcgtggcgct gcgctcccaa gccctgcgcg 14040ccctcgccgg ccacggcacc atggcctcgc tcgcgctgag cggcgcggag gccgagcgct 14100tcctcgcgga cctgggtgcg gcggcggcac gggtgacggt ggcggtgttc aacgggccgt 14160actccacggt ggtgtcgggg cccaccgacc aggtcgccgc ggtggtggcg gcctgtgagg 14220cggcgggcca ccgggcccgc acgatcgacg tggactacgc ctcgcacggc ccgcaggtcg 14280accggctcgc ggacacgatc cgcaccgact tggccgacct ctcccccggc gcctcggacg 14340cggtgttcta ctccgccgtc accggcgccc ggcagccgac ggaggagctg gacgccgact 14400actggttcac caatctgcgg cagccggtgc ggttcgcgtc ggcgatcgac gccctgctgg 14460ccgccgggta ccgcgtgttc atcgaggtca gccctcatcc ggtgctgatc cccgcgctac 14520gggagtgctt cgaggaggcc gaggtggcgg cggccaccgt gccgacgctg cgccgggacc 14580aaggcggccc ggatcaggtg gcgcgcgccc tgggggacgg cttcgtggcg gggctcgcgg 14640tcgactggag ccgctggttc gtcggcgacg ggcgtgaggc gggcgacgag ggccaccggc 14700cgcgtacggt cgagctgccc acgtatccgt tccagcggcg gcggtactgg ctggcgccgg 14760accacggccg ccgcgagggc cgcacggccg gggtcggaac ccggccggcc gggcacgccc 14820tgctgtcctc ggcggtcgaa ctggccgacg gcggcctggt gctgagcggc cggcttcccg 14880gcgacgcggc ctgggtgggc gcgcacaccg tggcgggcgt ccagttggtg cccggcgcgg 14940tcctggtgga ctgggcgctg ctcgccgccg acgaggcggg cggggcgtcg ctggaggaac 15000tgctgctgcg cgcgcccctg gagctgtccg ggccgtccgg gctgtccgag ccgtccgcgg 15060gtgtgctggc gcaggtcgcg gtcggggcgc ctgacgagtc gggccgccgt gagctacgga 15120tctcctcccg gcccgccgac gcgggcgcgg gcgagggctg gacgtgtcac gcggtggggt 15180cgctggcccc gggcggcccg cccgctccgg cggacaccgg gaccgcgacc gtcccctggc 15240cgcccgcggg cgccgaggcg ctggatccgg ccgggctgta cgagcgggcc gagcggcgcg 15300gctacggcta cgggcccgcg ctgcggggcg ttgtggcgct gtggcgggac ggcgccgacc 15360tggtggcgga cgtggcgctg cccgaggagg ccggtggcgg cggcgaaggc ggtgcggacg 15420gcgacggtac ggccggattc ggcctgcacc ccgtcctgct ggatgccgcg ttgcagcccg 15480cgctgctggc cgaaccggac ggcaccggtg gcgagggagc ggggccggaa gcccggttgt 15540ggctgccgtt cgcgtggagc ggcgtccggc tgtgggcgac cggggcgcgc gccgcgcggg 15600tccggctgtc gccgctggac gggggcggtg gggatgttgc ggacgagcgc gagctgcgga 15660tcgaggtgtc cgacccgacc ggggctcccg tgctgagcgt cgcgtcggtg gtgctgcgcc 15720cgcgcaccgt acggcaggtg cgcgaggcga gcggggccgc ggcgggtggt ctcttcgccc 15780tcgactggac gccggtggcg cctcaggagc cgtccggcgc cgaggacgac gccgggtgtg 15840tggccgtact gggcgaggcg ccgacggagc cgggcgtgga cgggtgccgg gacacctata 15900cggatctccc ggcgctgctg gccgccctgg acgcgggtgc gcccttgccc tccgtggtga 15960tgtggcggcc accggccgcc gaccccggcg ccgcacccga ggacgccgcc ctgtccgccg 16020tacgaggcgt ggcggcggcg ctgcgcgcct gggtcgccga gccgcggctg acggtcagcc 16080ggctcgcggt ggtcactcgg ggcgcggtcg cggcgggcgg cgccgagggc gagccggtgg 16140acctggccgc ggcggcggcc tggggctgtg cccgtggtgt ccaggcggaa caccccgacc 16200gcatcgtcct cgtcgacgtg gacgacgacg tcgacatggg cgcggacacc gacaccgaca 16260tcggcgcggc ggcgggtctg gcggccgcgc tcggcgaacc acaggtcgcg ttgcgcgggg 16320acacgctgct cgcgccacgc ctggcgcgtt ccgcggccac cccgggcggc gtggcattcg 16380acccgaacgg cacggtcctc gtcacggact ccggaggccc gctcgccggt tcggtcgccg 16440agcatctggt gcgcgccgag ggtgtccggc acctgctgct ggtccgcttc gagggcgcgg 16500acggcgccta cgacacgtat gacaggcaag acgcccaggt gcacatggtc acggttgatc 16560ctcgcgacac cgccgcgctg gaacgggtgg tggcgcaggt cgatccggcc catccgctga 16620ccggcgtcgt ccatgtcgcg ggactgtccg ccgacatcga gacaagtggc gcggcgcgcg 16680ggtgggccgt ggcggccggg gtggtgcggg ccctgcacca ggccaccgcc gcgctgccgt 16740ccgtccggtt tgtgaccctg tccgacgccg cgacggcctg ggacggcccg gcggcccccg 16800aaagggcggc ggccggtgcg ttctgcgcgg ccgtgacgga cgtacggcgc cgggccggac 16860tgcacggcct ggacgtcgcg ttcgggccct gggccgccgc cgacgacgat ggcggcgccg16920attccggtgg gcgctggacc ggggtcctcg gcgccgaccg cgggctcgcg ctgctgcgcg16980cggcctgtcg cgccgaccgc ccgcggctgg tggcggccga catccgtacc cgagccctga17040ccgcccatcc ggcccacgag ctcccggccg cgctgcgcac gctcggcgcg agtgcgagtg17100cgagtgcggg cggccgggcg ccggtccgtc gggtcgccgc cgccgcgccc ggccgcacca17160ccgactgggc gagccggctg gtgggcctcg ggcccgccga acggcgccgc gccgtcctgg17220agctggtacg cgatcacgcc gccgccgtgc tcgggcagcc cgaccccaag gccgtacggg17280ccgacgcctc gttcaaggag ctgggcttcg actcggtgac cgccgtcgag ctgcgcgacc17340ggctggtggc ggtcggcggg ctgcggctgc ccgccgccgt cgtcttccgc cacccgacgc17400cggaggcgct ggcccaccgt atcgagcagc aactcgcgcc cgacgacacg aataacgcag17460ctatcacaga caacgcagac aacgcagcaa agagcaacgg caacagcaac ggcacggcgc17520tcgacgcggc ggacaagctc gcgtcggcca cggccgacga aatcctcgac ttcatcgaca17580acgagctcgg cgtgctctcc gaagcgcgtc cgcgcccctc caactgaagg caggtgacga17640catggtcagc gaggagaaac tggtcgagta cctgcgccgg gtcaccaccg agctgcatga17700cgccaggacc cggctgcggg agctggagga gggcgagcag gagccggtcg cggtggtcgg17760catggcctgc cgtttccccg gcggggtgcg gtcccccgag gacctgcgcc ggctcgtcct17820gtcgggcggc gacgcgatcg gcgacttccc caccgaccgc ggctgggacc tggacggtct17880gttccacccg gacccggcgc acttcggcac cagctatgtg agccagggcg gcttcctgta17940cgacgtcgac cggttcgacg cggggttctt cgggatcagc ccgcgcgagg cgctggcgat18000ggacccgcag cagcggctgc tcctcgagct gtcgtgggag gcgctggagt ccgccggggt18060ggtgccgggc gcgctgcgcg ccagccggac cggggtgtac gtgggggtgt ccagcgagga18120ctacatctcc gggctgccgc agatcccgga gggcttcgag ggctacgcca ccacgggcag 18180cctcaccagt gtcatctccg gccgcgtcgc gtacaccttc ggtttcgagg gccccgcggt 18240caccgtggac accgcgtgct cgtcgtcgat ggtggccatc catctggcgg ggcaggccct 18300gcgacagggc gagtgttccc tcgcactggc gggcggtgtc acggtgctgt ccacgccgct 18360gatgttcacc gagttctgcc ggcagcgggc gctgacgccc gacgcccggt gcaagccgtt 18420cgccgccgcc gcggacggca ccggcttctc ggagggggcc ggactcctgc tgctggagcg 18480gctgtccgac gcgcggcgca acggccacga ggtcctggcc gtcctgcggg gctcggccat 18540caaccaggac ggcgcgagca acgggctgac cgcgcccaac gacgtcgccc aggagagtgt 18600gatccgggac gcgttggcga gggccgggct gtccggcgcg gatgtggaca tggtcgaggc 18660gcacgggacg ggtacccggc tgggcgaccc catcgaggct gaggcgctga tcgccacgta 18720cggggcggac cgcccggcgg accggccgct gtacctcggc tcgatcaagt cgaacatcgg 18780ccatacgcat gccgcggcgg gggtcgcggg cgcgatcaac accgtgatgg cgctgcggga 18840cggcaagctg gccaggaccc tgcacatcga cgagccgacc cgccacgtgg actggagcgc 18900gggcacggta cggctgctga cggacccgta cgactggccg gtggccgacc ggccgcggcg 18960ggcggcggtg tcgtcgttcg gggtgtccgg caccaatgcg catgtgatcc tcgaacaggc 19020cccggacgcc ggcgctcagc aggatgctcg gcaaaggggc ggcgacacgt tccacggcgt 19080ggtcccctgg cccgtttcgg ggcgcaccga ggcggcgctg cgggaccagg ccgcacggct 19140gggcgcgttc ctgacagcgg acggcgcgac ggcgaacggg gcggcgaccg gtggggtcgc 19200cgacgtgggc tggtcgctgg cgatgcgtcg cacggcgttc gagcaccggg ccgtcgtggt 19260cggccgcgac cggtcggacc ttctcgccgc gctcgaaggt ctcgcggccg acgagccggg 19320ccccgcggtg gtgcgcgggg tggcggcgga cgtcggcgcg ggcccggtca tggtgttccc 19380cgggcagggg tcgcagtggc tgggcatggg cgtggaactg ctggactcct cccccgtctt 19440cgcggcgcgt atcgccgcct gcgaacgggc cctggccgcg catgtcgact ggtcgctgac 19500cgatgtgctg cgcggcgcgc ggggcgcggc cgacatcggc cgggtcgatg tggtgcagcc 19560ggtgctgtgg gcggtcatgg tgtcgctggc cgcggtgtgg gaggcgcacg gcgtacggcc 19620gtcggccgtg gtgggccact cgcagggaga gatcgcggcg gcgtgtgtcg cgggcgcgat 19680gacgctggag gacggcgccc gcgtggtggc gctgcgggcg cgggcgctgc gggcgctggc 19740cggatacggc gccatggcct cgctgggctg cggcgtcgag gaaaccgagc ggctgaccgc 19800cgtacacgcg ccggacgtgg cggtcgcggc ggtcaacggc ccgtcgtcca cggtggtgtc 19860cgggccgtcc gagcaggtcg agaagctggt ggccgccgtg cgcgccgacg ggctgcgggc 19920ccgcgcgatc gacgtggact acgcctcgca cggccctcaa gtggaccgta tcgccgacga 19980gttggccgac gtactcgccg gggtgtccgg cgccgccacg gacaccgcct tctactcgac 20040cgtgaccggc gcccgtatgg acgcctccgg tctcgacgcc ggctactggt tcaccaatct 20100gcggcagccg gtgcggttcg ccgaggcggt ccaggcgctg ctcgacgccg attaccgggt 20160gttcatcgag gtcagcgcgc atcccgtact gctgctcggc cttcaggagt gcttcgaggc 20220ggcgggccga ccggccgtgg cgatcggcac gctccgccgg gacgagggcg gccccgagcg 20280gctgtgccgg gcgctggccg aggcgcatgt cgcgggcgtg gcggtggact gggcgagctg 20340gtacgccgat gggcccgcac ccgcggccgt accactgccg gcgtacgcct tccagcggga 20400gcggtactgg ctgccggccg gtgccgggtc cggtcctggc gatgtcgcgg gtgccgggct 20460caccgcggtc ggacacgcgc tgctcccggt gtccgtacgg ctggccgacg ggagcctggt 20520gctcaccggg cggctgccgg aggcggcgcg ggccggctgg ctggccgaac acctcgtcgc 20580ggacctcccg ttgctgcccg gcacggtgct ggtcgaatgg gtgctgcggg cggccgacga 20640ggccggctgc ggtggtgtcg aggagctggc gctccaggtc ccggtggcgc ttcccgtatc 20700cggcgggctg gtgatccagg tggtcgtgga cgccgccgag ggcgacggac gccgtccggt 20760gcgggtccac tcccggcccg aggaggactc gggcgcgccg gacgcatggg tctgccatgt 20820ctcgggcacg ctgctccccg gcgtggccgg gccggttccg ccgtccggtc cgggcggcgc 20880gtggccgccg ccgggggcgc ggcccgcggc gatcgacggc ttctacgagc gggccgaggc 20940cgcgggttac ggctacggcg cgttcttccg gggcctgacg aacgtatggc acgacggcga 21000ggacacgctg gcggaggtgg tgctgcccaa ggaggcggcg gagcaggcgg gtggcttcgg 21060tatccatccc gccctgctcg acgcggcgat gcagcccgta ctgctggccg gtcaactccg 21120tcaatgcgct gccgcggcgg gcgcggacac ggcgtccggg accgtgctgc tcccgttcac 21180ctggagcggg gtgcggctgt gggcgggtgg cgccacccgg ctccgcgtcc ggctgtcgcc 21240gcggccggag gggctgcggg tgctgttggc cgatgccacg ggtgcccccg tactgaccgc 21300ggacgcggtc gccctccgcg agacgggcgt ccagcagttg cgcgcgtcga gccgggtgcg 21360ggggtcgcac gggctgttcg ccgtcgaatg ggtgccgccg ctgtccgcta cggcgggcgg 21420gacggcgccc gcgacgctcg cggtgctggg cgacgacgcc ccggacctgg ccgatgccga 21480ccgttacccc gacctcgacg cgctgttccg cgccgtggcc gacggcgccc ccgcaccgga 21540tgtcgtcatc gcttcggtgc ggacgggcaa cgacccggcg gggtcggaca ccggcttggc 21600caccgcccgc cggacgctga cgctggctca ggagtggctg gccgggagcg gggccgacgg 21660tgcgcggctt gccgtggtca cccggtcggc gatacggacc ggggacgacg gccaggagcg 21720ggtggtcccc tcggccgccg cggtgtgggg gctgatgcgc agcgcccaga cggaacaccc 21780ggggcgtttc gtcctcatcg acgaagacac cgacagcacc gaaaacatcc tggaggccgt 21840acgtacggac gaaccgcagc tcgcgctgcg cggcgggcgc gcgttggtgc cccggatggc 21900ccgggtggac gccgaaccgg agctgacggc cccgtcgggc gagcgggcct ggcatgtggc 21960ggccggcaag accggccccg acgatctcac ggcggtgccc agcccccggg cctccgcgcc 22020cctcgcgccc ggccaggtgc ggatcgccgt acgcgccgcg gggctcaact tccgcgatgc 22080gctgatcgcc ctggacatgt acccggacgc ctcggcgtcg atcggcagcg agggcgcggg 22140ggtcgtgctc gaagtgtccg agggggtggc cggggtcgcc gtcggcgacc gtgtgatggg 22200cctgttcaac gacgcgttcg gcccggtggc ggtggcggac gcccggatgg tcgccccggt 22260gccggacggc tggagcttcc gggaggccgc ggcggccccg gtggccttcc tgaccgcgtg 22320gtacggcctg gtggatcttg gcgggctgag ctccggcgag accgtggtga tccacggtgc 22380ggccggtggc gtgggcatgg ccgccgtcca ggtcgcccgg cacctgggcg ccgaggtgtt 22440cgcgacggcg agcccggcga aacacccggt gcttgagggc atgggcgtgg acgccgccca 22500ccgagcgtcc tcgcgggatc tggggttcga ggcggcgttc tcgtcggcga ccggcggccg 22560gggcgtcgat gtcgtcctca acagcctggc cggggagttc acggacgcct cgctgaggct 22620tctcgcgccc ggcgggcggc tgatcgagat gggcaagacg gacgtacgcg atccggatca 22680ggtcgcgcgc gagcactccg tggcgtaccg ggcgttcgat ctgatcgcgg acgccggccc 22740ggagcgcatc gggcagttgc tggccgccct gggtgagcgg ttcgccgacg gcgcgttcac 22800gcccctgccc gtcaccgggt ggcggctcgg ccaggccagg caggcgctgc ggcagctcag 22860ccaggcccgg cacaccggga agctggtgct ggacgtggat cccgcacccg acccggacgg 22920cacggtgctg atcacgggcg gcaccggcac tctcggcggt ctgatcgccg aacacctggt 22980gcgctcgcgc ggggtgcgcc atctgctgct gctcagccgg cgcggcccgg acgccccggg 23040cgccgaggag ctgacggccc ggctcaccga gctgggcgcg cgggtgcggg tggccgcggt 23100ggacgtcggg gacgccaccg ccctgggcga ggcggtcgcg ggcgtggacc cggcgcatcc 23160gctcaccggt gtcgtccacg cggcgggcgt ggtggccgac gcgatgctgc catcgcagga 23220cgacgaacgc ctggtggcgg cctggtccgc caaggccgcg gcggcggccc gtctgcacga 23280cgcgaccgcc gggctgccgc tgggcatgtt cgtgctgttc tcgtcgttcg cgtcgaccct 23340cggcaccgcg gggcaggcca actacgcggc cgccaacgcg tactgcgacg ccctggtcga 23400gcggcggcac gccgagggcc tgccgggcgt atcggtgagc tgggggctgt ggtccgccgc 23460gagcgggctc accggagggc tgaccgaggc cgatgtcgcg cggatcgccc gccagggcat 23520cgtcccgaac agcaccgagc agggatacga cctgttcgac gcggcgctcg gacacgggcg 23580tcccgcgctg ctcgcgctga acctggacac ccgggcgctg gccgcgcagc ccgtcgcggc 23640cctgcccgcg ccactgcgcg ccctggccgc cgacgcccag gcggcgggcg cccgctccgg 23700cggcgcggcg gcgcggccga ccgcggcggc ggccgaggag cccgcggact gggctgcccg 23760gttgcgcgcg ctcgccccgg ccgagcagcg gcgcctgctc accgacctgg tacgcaggca 23820cgccgccacc gtgctcggcc acgccgaccc cgaggccgta ccggccgacg cggcgttcaa 23880ggagctcgga ttcgactcgc tgaccgcggt cgagctgcgc aaccgcgtca ccgccgccac 23940cgggctgcgg ctgcccgcca cggtcatctt cgactacccg gagcccgggg cgctggccga 24000gcgtctgcgc accgaactgg cccccgagga gggggcatcg gcgacggcgc cggacctcta 24060cgcgcccgtg ctcagccgac tcaccggcct ggaggagacc ctggcggcac tggcctcgtc 24120cggtgtcaac ggcggtgtga atggcggagt ggccgacccg ggcgcggtga ccgcgcgtct 24180ggagtcgctg ctggccgact ggaaggcggc ccacgccccg agccggaacg gcggcacggc 24240ggccgaacgg ctcgaagccg cgacgaccga tcaggtcctc gacttcatcg acaaggaact 24300cggagtgcaa tgagcggggg cgctgtgacc actgagacga acgaagagcg gctggtcgat 24360tacctcaagc gggtcgccgc ggatctgcac gacacgcggg cgcggctgcg cgaggtggag 24420gacggacagc gggaaccggt ggccatcgtg gcgatggcct gccgctaccc cggcgacgtc 24480gcctcccccg aggatctgtg ggacctcgtc gccgcgcgcc ggcacgcgat gaccgcgttt 24540ccggacaacc gcggctggga cctggagcgg ctgttccacc cggaccccga ccacccgggc 24600accagctacg cccgcgaggg cggattcctg cacgacgccg acctgttcga cccggagttc 24660ttcggcatca gcccccgcga ggcggcggcg gtcgacccgc agcagcggct gctgctcgaa 24720gtggcctggg aggcgctgga gcgggcgggc atcgcgcccg ggtcgctcaa gggcgcgccg 24780gtcggcgtgt acgcgggcac cgcgctgccc ggcttcggca ccccgcatat cgaccgggcc 24840gcggagggct atctggtcac cggcaacgcg ccgagcgtgc tgtccggccg ggtggcgtac 24900accctgggcc tggaggggcc cgcggtcacg gtggacacgg cgtgttcgtc gtcgctggtg 24960gcgatgcatc tggccgcgca ggcgctgcgg cagggcgaat gcgagctcgc gctggcgggc 25020ggtgtgacgg tcatgacgac gccttatgtg ttcacggagt tcgcccggca gcgcggcctg 25080gcggcggaca gccggtgcaa ggcgttctcc aagggggccg acggcacggc gttcgccgag 25140ggcgcggggc tgctggtgct ggagcggctg tcggacgccc agcgcaacgg acatcaggtg 25200ctggccgtga tgcgcggttc ggcgatcaac caggacggtg cgagcaacgg gctgaccgcg 25260cccaacggtc tggcgcagca gcgcgtcatc cggcaggcgc tggccggcgc ccggctctcc 25320ccggcggatg tggatgtggt cgaggcgcat ggtacgggca ccacgctcgg tgacccgatc 25380gaggcccagg cgctgctggc cacctacggt caggagcggc ccgagggccg gccgctgtgg 25440ctgggcgcga tcaagccgaa cctcgggcat acgcagggcg cggcgggcgt ggccggcgtg 25500atcaagatgg tgatggcgct gcggaacgcc tcgctgcccg ccctgctgca cgccgaccgg 25560cccacgtccg tggtcgactg ggacggtggc gcggtacggc tgctggccga gccggtggcg 25620tggccggccg gggaccggcg gcgccgggcc ggcgtctcgt ccttcggtat cagcgggacc 25680aacgcgcatc tgatcctcga ggaggccccg ccacggccgg acacggagct gcccgcgagc 25740gtgcctcagc ggccggaggg caccgtcgtg ccgtgggtgg tctccgcgcg gggcgcggtg 25800tcgcttcgta cgcaggccgc ggcgctcgcc gagcacatgg ccgcgcaccc ggacaccccg 25860gtcgacgcca tcggctggtc gctggccacc acacggtcgc cgctcgacca ccgcgcggtc 25920gtgctgggcg cggaccgcgg ggagttgtcc gcccggctcg ccgacctcgc cgaggggcgg 25980acccaccccg atgtgacgcg ggcgtccgcc ccggcgcggc tcggtggcag cgcgtttctc 26040ttcaccgggc agggcagcca gcgccccggc atgggcgccc agctgtaccg ggcctatccg 26100gcgttcgccg ccgcgttcga cgaggcgtgt gcggcgctcg acccccatct ggggcggtcg 26160ctgctggagt tggtgttcgc accggccgac acagacggag acggagatgc ggacagggcg 26220agcgcgctgg acgcgacgga ggtgacccag gccgcgctgt tcgcggtcga ggtcgccctg 26280taccggctgg tcgagtcgtt cggtgtggtc cccggctatc tggcgggaca ctcggtcggc 26340gaactggtcg ccgcgcatat cgccggggtg ctgtcgctgc cggacgccgc gcggctggtc 26400gccgcccggg ggcggctgat gcaggcgctg cccgagggtg gcgcgatggt cgcgttggag 26460gccgccgagg aggaggtggc gctgctgctc gccgggcggg cggaccaggt ggcgctggcg 26520gccgtgaacg cgccgacgtc ggtggtggtc tcgggtgacg aggaggcggt cgaggagatc 26580gcccgtacga tccgggagcg cggacaccgg accaggcggc tgcgggtcgg tcacgccttc 26640cactcgccgc ggatcgaccc gatgctggag gagttccggc aggtcgcggc ctcactgacg 26700tattcacagc cccggatcgc cgtcgtctcg aacgtcaccg gcgcgctggc cggggccgag 26760cagctgtgcg accccgacta ctgggtgcgg catgcgcggc agccggtgcg gttccgcgac 26820ggtatcgccg cgctgcgcgc ggagggtgtc acccgcttcc tggagctcgg cccggacgcc 26880gtgctcaccg ccatggcgcg cgactgcctg acctcggagg cggccccgga ggtggccgcc 26940ggggaatcgg cccaggatgc cggcacggcc tccggtccca gcgcgccggt gctcgccacg 27000gtgttgcgca agggccgcga cgagccccgt acgctgctga ccgccctcgc ccagctccat 27060gtggacggcg agagcgtcga cttctccgcc tccttcccgg ccaccaccca ggccaccgac 27120ctgcccacct accgcttcga ccgccgccgg tactggcggg acgccccgca ggcggaggcg 27180gacgtacggg ccgcggggct cgaggcgagc gaccatccgc tgctgcgcgc cgcgctcgaa 27240ccggcggacg gcgggctgct gctgaccggc cggctgtcgc tgcgcggcca gccctggctg 27300gccgaccacg ccatcgtgga cgcggtgccg ctgcccggca cgctcttcgt ggagctcgcc 27360ctgcaggccg gggaacgggt cggctgcgac ctgatcgacg atctcaccct ggaggcgccg 27420ctgctgctgc ccccggtggg tgcggtggac ctccaggtgg ccgtcggggc caccgacgcg 27480gccggccgac gggccgtcac cgtctactca cgcccgtccg gcggcggttc ggagggttgg 27540gagagttcgg cggacccggg tgtcgcggac gagccgggcg acggcccgta cggcccgtgg 27600cgccggcatg ccacggccac tctgggcacc gcgccgaccg gtgtccccga gcccgccgcg 27660tcaccggcgc agtggccgcc cgccggggcg gaggcgatcg acgtggccgg gctgtacgag 27720cggctggccg ccgagggcta ccggtacgga ccggcgttca cggggttgcg caccgcgtgg 27780cgggtcggcg aggagatgtt cgccgaggtg ggtctcgccc ccggtcagcg cggcgacggc 27840ggggcgtacg ccgtccaccc cgcgctcctg gacgccgccc tgcacccgat cggcgcgctg 27900ttcaccggcg aggaccaggc gggcggcgcg ccgggcacgg tccggctgcc gttctccttc 27960ggcggcgtac ggctgctcgc gcgcggcgcg tcccggctgc gggtccggat cacgccgacc 28020ggacctgaca ccgtgaccat gcggctgtcc gacgacaccg gcgccgaggt cgtggccgtc 28080gactcgctca cgctgcgcac cgtttcggcg cagcggtggc ggtccggggc ggtcccggcg 28140gaccggccgc tgtaccggct ggactgggat gccttcgcgc tgcccgccgc cgcgacaacg 28200gcgccggacc gctgggccgt actcgccgcc gatgacacga acgccacgga caccgccacg 28260gcctcgctgc ccgcggaaca cgtcgcgcgc caccccgacc tcgccgcgct gagcgcgtcg 28320gtcgccgccg gggcccccgc ccccgacctg gtcgtcatgg cctgcctcgg tgccccctac 28380gacacgtccg acgacggcga cgagccgccc tcccaggtgc gcaccgccac ccatcgtgtc 28440ctcgcgcggc tgcgggagtg gctgacggac gacgccctcg ccgcctcccg tctggtggtc 28500ctgactgcca aagccgtcgc cgccgacccg gccgacgcgc cgcccgatct ggcgggcgcg 28560gcggtgctgg gcctgctgcg cgccgcccag gccgaacacc cggggcggat cgtgctggtg 28620gacaccgacg gcatctcggc gtcccgggac gcgctggcgg cggccgtggc tgccgccgtg 28680gccgccggtg agtggcagct ggcgctgcgc gacgggcgcg cgctggtgcc ccggctgatc 28740ctggcgcacc ccgaccccga cgcggcgccg gtggtgctcg accccgacgg caccgtgctg 28800gtgaccggcg gcaccgggtc gctcggccgg ctgctcgccc gccacctcgt cgaacatcac 28860ggcgcccggc atctgctgct ggtgagccgg agcggaccgg ccgccgaggg catcgaggcg 28920ttcgcggccg ggctcgcggc cgacgtacgg atcgagagct gcgacaccac cgaccccgag 28980gcgctggccg ccctgctcgc caccgtcccc ggcgaacacc cgctgaccgc ggtcgtgcac 29040accgcgggcg tgctcgacga cggcgtggtc acctcgctga cgcccgaaca gctcgacacc 29100gtactggcgc ccaaggtgga cgcggcctgg cagttgcacc ggctgacccg gggcgcggac 29160ctcgcctcgt tcgtcctgtt ctcgtccgcc gcctccgtgc tcggcagcgg cgggcagggc 29220aactacggtg cggcgaacgc gttcctcaac gccctcgccg agcacatccg cgccgcgggc 29280ggcccggcca cctcgctggc ctggggcctg tggggcgtgg acgaggggat gacggaacac 29340ctggccaccg cggaccgggc ccggatggcg cgttcgggca ccgccgcgat gtccggggag 29400gcgggcctgg cccggttcga cgcggcgctg gccaccgcgc tgcccgtact ggtcccggcc 29460aggttcgacc tcgccgtgct gcgcgaacag gcggccggcg gcgcgctgcc tccgctgctg 29520cgccgcctcg tacgcctgcc cgtccgtacg gccgcggcgg tggaggcgtc cccctcctgg 29580gccgggcggc tcgccgggct gccggagacc gagcaggacc gggtgatcgg ggagctgata 29640cgcgaccgga tcgccgcagt cctggcgcat ccggagcccg aaaccctcga actgggccgg 29700acgttcgcgc agctcggcct ggactcgctg accgcgctgg agctgcgcaa cgcgatccat 29760gaggcgacgg gggtccgcct cccggccacc gccatcttcg actacccgac ccccgagacc 29820ctcgtcagcc atctgcgcac cgaactgctc ggcgccaccg cgaccaccgc ggccaccgcg 29880ccgctgccgc cgggggccgg ggccccggcc cggtccggga gcgccgacga ccccgtcgtg 29940atcgtcggca tggcctgcca ctacccgggc gatgtgcact cccctgacga gctgtggcgg 30000ctggtggccg acggggtgga cgcgatcggg ccgttccccg aggaccgtgg ctgggatgtg 30060gccgggctgt acgacccgga ccccgagcgc accggcaaga gctacaccag ggagggtggc 30120ttcctgcccg aagcggccct gttcgacgcg gagttcttcg ggatcagccc ccgggaggcg 30180ctggccaccg atccccagca gcggctgttg ctggagacgg cgtggcaggc gttcgagcac 30240gcccgtatcg atccggcggc gctgcgcggc agccgtacgg cggtggtcac cggcatcatg 30300tacgacgact acggcgcgcg gttcctgggc cggatcccgg aggggtacga gggccagatc 30360atgaccgggt ccacgccgag cgtggcgtcc ggccgggtgg cctacacctt cgggctggag 30420gggccgacgc tgaccgtgga cacggcgtgt tcgtcgtcgc tggtggcgat gcacctggcg 30480gcgcaggcgc tgcgccaggg ggaatgcgac ctcgccctgg cgggcggtgt gacggtgatg 30540gccacgccga acacgttcat cgagttctcg cgccagcgcg ggctggcgcc ggacgggcgg 30600tgcaaaccgt tcgctgccgc cgccgacggc accgggtgga gtgagggcgc cgggctgctg 30660gtgctggagc ggctgtcgga cgcgcgccgc aacgggcacc gggtgctcgc ggtcctgcgg 30720ggttcggcgg tcaaccagga cggtgcgagc aacgggctga ccgcgcccaa cgggccgtcc 30780cagcagcggg tgatcggcca ggccctggcc gcggcggggg tggatcccgc cggggtcgat 30840gtggtggagg cccatggcac gggcacgatg ctgggcgacc ccatcgaggc ccaggccctg 30900ctggccacat acggccggaa ccgccccgca gaacagcccc tatggctggg gtccatcaag 30960tcgaacatcg gccacaccca ggccgccgcc ggggccgcgg gcatcatcaa gatggtcatg 31020gccctgcgcc acggccggct gcccgccacc ctgcacgtgg acgagccgag cccgcatgtg 31080gactgggcga gcggaagcgt ccggctcctc accgaggcca ccgactggcc ggaggccgac 31140cggccccgcc gcgccgccgt ctcctctttc ggcatctccg gcaccaacgc ccacctcatc 31200ctggaacagg cccctgacca gcccgaaccg ctcccggaac agtccgagtc agccaccgcg 31260ggcggcatcg tgccgttcgt gctctccgcc cgtacggccg aggcgctgcg cgcccaggcc 31320gcgaacctgg cggcgcggct cccctcggcc ggggtggcgg aggtgggctg gtcgctggcg 31380accacccggt cggcgttcga gcaccgggcg gtgatcgtgg ccgaggaccg ggacgcgctg 31440ctggccggtc tggagaagct ggccgccgac gagcccgatc cggcggtggt ggcggggacg 31500gcgaccaccg cggccgcggg cccggtgttc gtcttcccgg gccaggggtc gcagtggcgc 31560ggcatgggcg tggaactgct ggacacctcg ccggtgttcg cggcccggat cgcggagtgc 31620gagcgggccc tggcgccgta cgtggactgg tcgctcaccg ccgtactgcg cggcagtgac 31680accaccaccg acccgcaccg cgtggatgtc gtccagccca ccctctgggc cgtcatggtg 31740tccctggccg ccctctggca acacctgggc atcgccccgg ccgccgtcat cggacactcc 31800cagggcgaga tcgccgccgc ttgtgtggcg ggagccctca ccctcgacga cgccgccaag 31860gtcgtggccc tgcgcagcca ggcactgcgc gccctcgccg gccacggcac catggcctcc 31920ctcaccctcg gcgccgacga caccgccggg ctcctggagg agctgggcga gcgggccgac 31980gacgtcaccg tggccgccgc gaacggcccc gtcaccaccg tcatctccgg tgcggtggag 32040cagatcgcca ccgttctggc ggcggccgag gcgcacggcg cgcggacccg caccatcgat 32100gtggactacg cctcccatgg gccccatgtc gaccgcatcc gcgaggacat cgtctccgcc 32160ttgagcggcc tcgcgcccac cgcgtcggag gtcgccttct actcgacggt gaccgccgaa 32220cgcctggaca ccgccgggct cgacgccgac tactggttca ccaacctccg ccgcccggtc 32280cgcttcgccg acaccctcgc cactctcctc gcccatggac accggcactt catcgaggtc 32340agcccgcacc ccgtgctgat ccccgggatg caggacggtt tcgaggcggc cgacgccgcc 32400gccaccgccg tccccaccct ccgccgcgac cagggcgggc cccaccagct cgcccaggcc 32460gtggcccgtg cgtacaccgc cggtctggcc atcgactggg ccccctggta tccggcccgg 32520ccgtacacca ccgacctgcc cacctacccc ttccagcgcc gccgctactg gctcggcatg 32580gacggcggac cgggcgatct gcgcagcgcc gggctcgtct cggtgtccca cgcccagatc 32640ggcgccgcgg tcgaactggc cgacggcgga ctggtgatga ccggccggct gcccgccgcc 32700gggagcggcg gctggctgga cgaccacgtc gtcgccgaca ctcccctggt gcccggcact 32760gccctggtgg agtgggtgct gcgagcggcc gacgaggcgg ggtgcggcgg gatcgaggag 32820ttggcgctcc atgtcccgat gacactgccc gcgtccggtg ggctgcggat ccaggtcgtg 32880gcgtacgcgc cggacggcga cggccgccgc gaggtacggg tgcactcccg ccccgacgcg 32940gaggacggct cgtcaccctg gacctgccac gccaccggac acctctcccc gaccgccccg 33000ggcgccgcgg acccggcccc ggccggggtg tggccgccgc gggacgccga acaggtcgac 33060gtggccgact tctacggccg cgccgaggcg atcggctacg gctacggacc ggccttccgg 33120ggcctgaccg ccgcctggcg gcagggtgac gacctgctcg ccgaggtggt gctgccggag 33180gcggcgcacg agggcgccga cgggttcgcc ctgcaccccg cgctgctcga cgcggccctg 33240caccccctgg ccctggacgg gcagggggag gacgggcgga tgcggctgcc gttcgcctgg 33300agcggtgtgt cgctgtgggc caccggcgct cgcgcggccc gggtccggat gtccccgctg 33360gaacacggct tccggctggt ggtggccgat gcggcgggcc ggccggtgct cagcgccgaa 33420tccgtggtcg tacgccccac cagcgcacgg cagttgcgcg acgcgggcgc gcggcgggtg 33480gacgggctgt acgaggtggc gtgggtggcc ctgccgccgt cgtcggacac cgtcgctgag 33540acggagacgc ggggcgtaga ggggtgggcg ctgctggacg gcggcccgct tccgttcgac 33600ccgtcaaagg ccggatccct gcctcgccac gccgacatcg acgccctgct gaccgccccc 33660gccctgccgt cgacagtgct cgtcggcgtt tccggccccg tgggcgccgc gggcgacgag 33720aattcggccg agggcgcgtt ggccgtgacc accggtgtgc tgacgtcggc gcggcggtgg 33780ctggagacac cggagctggc cgacgcccgg ctggtgctgg tcacccgggg cgcggtcgcg 33840gctgcggaaa ccgacgacgg ccccgaccct gcggctgccg cggtgtgggg gctgctgcgc 33900agcgcacagg cggagaaccc gggccgtttc ctgctgtgcg acatcgacga cggcgccggg 33960cccgacgatg tgctcggcgc cgtcacacgg gcggtggccc tggacgagcc gcaggtggcg 34020gtgcgcggtg agcgtgtgct gacgccccgt cttgagcggg cgggagccgc tgagttggta 34080ccgccgccgg gtgagcctgc ctggcggctg tcggccgatg acaccggcac catcgacagc 34140gtgtcggtgg tggcctgccc cgaggtgctg gagccgctgg cgcccgggca ggtgcggatc 34200gcggtgcggg ccgcgggcat caacttccgc gatgtgctga tcgtgctcgg gatgtatccg 34260gacgaggggg tgttccgggg cagcgagggc gcgggcgtgg tgctggacgt ggccgacgat 34320gtgacgtcgg tggccgtcgg cgaccgtgtc ttcgggctgt tcgagggcgc cttcgggccc 34380gtggccgtcg ccgacgcccg cgccgtcgta ccggtcccgc cggactggac cgaccagcag 34440gccgcggccg tcccgacgac gttcctgacc gcctggtacg ggctggtgga cctggccggc 34500ctcaaggcgg gcgagtcggt cctcatccat gccgccacgg gcggggtggg cacggcggcc 34560gtgcagatcg cccgccacct gggcgccgag gtgtacgcca ccgccggccc cggcaaacac 34620gccgtactgg aggcgatggg catcgacgag gcccaccgcg cctcctcccg cgacctggac 34680ttcgaggacg ccttccgcac cgccaccggc ggccgcgggg tcgacgtgat cctcaacagc 34740ctcgcgggcg agtacaccga cgcgtccttg cggctgttga ccggtggtgg gcgcttcatc 34800gagatgggca agaccgacaa gcgtgacgcc gagcagatcg ccgacacgta ctccggcgtc 34860cgctaccgct tctacgacct ggtgccggac gccggtctgg accgggtcgc ggagatgctg 34920accacgctgg ccgggcactt cgcccagggt gttctggcgc cgccgccggt gcgggcgtgg 34980ccgctgacgg aggcccggca ggcgctgcga cagatgagcc aggccaggca caccggcaaa 35040tacgtcctgg acatgccccg tacgctcgac ccggacggca ccgtcctgat caccggcggc 35100accggcaccc tcggcgccct cgtcgccgaa cacctcgtca ccaaccacca catcagccat 35160ctccacctcc tcagccgccg cggacccgac gcccccggct ccgccgacct ggcggcccgc 35220ctcaccgaac tcggcgccac cgtccgcatc accgccaccg acaccaccga cccgcaggcc 35280ctccggcagg ctctggacac cgtcgaccgc gaccatccgc tcaccggtgt catccacgcc 35340gcgggggcgc tggacgacgc ggtgctgacc gcgcagacac cggagcggct ggcgtcggtc 35400tgggcggcga aggcgacggc cgcggccaat ctccaccggg ccaccaagga cctccccctg 35460gccatgttcg tgatcttctc ctcggccgcc ggcaccctcg gcacaccggg gcaggccaac 35520tacgccgccg ccaacgccta ctgcgacgcc ctcgccgtcc ggcgacggcg ggcggggctg 35580cccgccacct ccatcgcctg ggggctgtgg gccgccacca gcgagatgac cgggcatctg 35640gcggacgccg atctggcccg gatgagccgc accggcttca ccccgctggc caccccgatg 35700gcgctggccc tgttcgacgc cgccgggcga cacggcgccg cgaccccgct cgcgctcgac 35760ctcgatccgc gtacgctcgg cgcccagccg tccgacgccg tgcccgcggt gctgcgtacg 35820gtcgccgccg cgggagcgcc ggtccggcgt accgccgccg tcgcacagtc caccgactgg 35880gccggccgac tggccgcgct ctcggccgcc gagcgccacc gcgagctggt caacctggta 35940cgtacgcatg cggcgacggt gctcggccac agcgaccccg cggcgctccg cgcggacacc 36000tcgttcaagg agctggggtt cgactcgctg accgccgtcg agctgcgcaa ccggctctcg 36060gccgcgaccg gtctgcggct gcccgccgcg ctcgtcttcg actatcccga cgcggagacc 36120atggcccgct tcctggacca gaagctcgcc cccggggacc ggacggaggc ggcggcggtc 36180gaccacctcg cccccgtcct gaacgacttg gcccggctgg agtccacctt gggcagccat 36240gacgtggacg ggaaggcccg cgagacggtc gcgggccggc tccacgccct gctgtcccgg 36300ctggagggga gcacggccag tgcggcggac atcgacggcg aggcgctgga gtccgcctcg 36360gacgacgaga tgttcgcgct catcgaccag caactgggct cgtcctgacg agaagtgggg 36420gaggaccaat gtcgtccacg gaagacaagc tgcgccagta cctcaagcgg gtcaccgtcg 36480acctgggcga ggcccgagcg cgcctgcgca aagcggagca acgccaacac gagcccatcg 36540ccatcacctc catggcctgc cgctaccccg gcggtgtcac ctcccccgaa accctgtggg 36600agctggtcga cagccgcacc gacgccatcg gctccttccc ggccaaccgc ggctggaacc 36660tggcctccct ctaccacccc gaccccgacc actccggcac cagttacgtc cgggacggcg 36720gcttcgtcca cgacgccgat gaattcgacg cctccttctt caacatcagc ccgcgcgaag 36780ccctcgccat ggacccgcag cagcggctcc tcctcgaaac cgcctgggaa ctcctcgaac 36840gcgcccatat cgaccccacg gccctcaagg gcacccccac cggggtctac acgggctgtg 36900gtgtgcccgg tttcggcaca ccacatatcg agcggagcgc cgagggcttc ctgctgaccg 36960gcaacgcgct cagcgtggtg tccggccgta tcgccttcac cctcggcctg gagggcccgg 37020cggtcacgct ggacacggcg tgctcgtcct cgctggtcgc gatgcacctg gcggtccagg 37080cactgcggca gggcgaatgc gacctggcgc tggccggcgg ggtgacggtc atgtcgaccc 37140cgaacgtgat cgtcgagttc tcccggcagc gcggcctgtc cccggacggg cggtgcaagc 37200cgttcgccac ggcggccgac ggcacgggct tctccgaggg cgcgggcctg gtgctgctgg 37260agcggctgtc ggacgccgag cgcaacgggc accaggtgct cgccgtcatc cggggtacgg 37320ccgtcaacca ggacggcgcg agcaacgggc tgagcgcccc caacggaccc tcgcagcagc 37380gggtgatccg ccaggcgctg gccaacgccg ggctggccac ggtcgaagtg gacgcggtgg 37440aggcccatgg cacgggcacc acgctcggcg acccgatcga ggccgaggcg ctgctggcca 37500cctacggtca ggagcggccg gaggaccgtc cgctgtggct ggggtcgatc aagtccaaca 37560tcggccatac gcagggcgcc gccggggtcg cgggcgtcat caagatggtg atggccatgc 37620gccacgcctc gctgcccgcc accctgcacg tcgacgagcc cacgtcgcat gtggactggg 37680accggggcac ggtacggctg ctgaccgagc cggtcgactg gccgacggcc cccgaccggc 37740cgcgccgggc cggtgtctcg gcgttcggca tctccggcac caacgcccac atcatcctgg 37800aggaggcggg cctgcccacg gcggcggagg cggaggctgg gactgcggct gaggctggga 37860ctgacgctgg ggctgggact gaggctgggg ctgaggacgc cgcaccggag gaggccaccg 37920tagagccggc cctcctgggc ggcgtggcgc cctgggtggt ctcggcccgt acccaggagg 37980ccctggccga ccaggcgcgc gggctggtcc gcgccgtcac cgacaccggc gccccggatg 38040ccgtgcccgc cgaggtggcc tggtcgctgg ccaccacccg cgccacattc gaccaccgcg 38100ccgtggtcac cggcaccgaa ctcgccgacc tcaccgccgc gctcgaggcc ctggccaccg 38160gcggtgaaca cccccatctc gtccgaggca ccgccctcga cccgcaggcg ggcccggtcc 38220tcgtcttccc cggccagggc tcccagtggc ccggtatggc cgtcgggctg ctggactcct 38280cccccgcctt cgccacccgg atcgccgcct gcgaacaggc cctggccccg tatgtcgact 38340ggtcactcac cgccgttctg cgcggcagtg acaccgcaac cgacccgcac cgcgtggatg 38400tcatccagcc gaccctgtgg gccgtcatgg tgtccctggc gggcctgtgg caggacttcg 38460gcatcacccc ggccgccgtc atcggacact cccagggcga gatcgccgcc gcttgtgtgg 38520ccggggccct cagcctcgac gacgccgcca aggtggtggc actgcgcagc caggcgctac 38580gcgccctcgc cggccacggg gccatggcct ccctcaccct cggcgccgag gacaccgccc 38640gcgtcctgac cggactcggc ccggccgccg agggcgtcgc ggtcgccgcc cacaacggcc 38700cccgctccac cgtcgtctcc gggccaccgg accagatcgc caccgtcctg gcggccgccg 38760aggcacgagg ggcgcggacc cgcaccatcg acgtggacta cgcctcccac agcccgcacg 38820tcgaccgcat ccgcgacacc atcctcgcgc aactcgccga tctggccccc gccgcgccca 38880ccatcccctt ctactccacc gtcaccggcg agccgctggc cgacaccccg ctcgacgccg 38940agtactggtt caccaacctc cgccagccgg tccgcttcac cgacaccctc accaccctcc 39000tggaccacca gcaccgccac ttcatcgagg cgagccccca ccccgtcctc acccccggca 39060tccaggacgc catcgacgac gccgagctcc ccgccaccac gatccccacc ctccgccgcg 39120accacggcac cccccacgac ctcgcggacg ccctcgcgct cgcccacacc accggcctcg 39180ccgtcgactg gcggccctgg tacgccacca ccccgcccgc caccaccgat ctgcccacct 39240accccttcca gcggcagcgc tactggtcgg cggctggccg ccggaccggc gacgtcagcg 39300cggccggtct gcggccggtg gaccatccgc agctgtccgc cgcgacgggc ctcgccgacg 39360gcgggctcct gctcaccggg cgcctgccgg ccgccggtga cgcgggctgg ctgggcgagc 39420acgagttcgc cgatgtggtc ctggtgccca gcacggccct ggtggagtgg acactgcggg 39480cggccgacga ggcgggctgc ggcggcgtcg aggagctgac ccttgaggtc ccgctgaccc 39540tgtccgccgc ctccgagcta cgggtccagg tcgtggtgga cgcccccgac gaggacggcc 39600gacgcgccgt acgcgtctcc tcccaacccg cggtggacac cccggaccgg gccgacgggc 39660aggacacctg gacctgccat gcgacgggca ccctcatggc cgccgcagcc gctgggaccg 39720agctggcggg cgcctggcca ccggccggtg ccgaacctgt ggacctcacc aacctgtacg 39780cacgcgccga ggccgccggc taccggtacg ggccgacgtt ccagggtgtc caggcggtgt 39840ggcggcacgg cgccgacctg ctcgccgaag tggccctgga ccagggggcc gaggagggcg 39900gcgacgagtt cggcatccat cccgcgctgc tcgaatgcgc ccttcacccg gtggcgctga 39960ccgatacacc gcacgacgat acgccgctgg gcgatgcgga caccgacggt ccgctgtggc 40020tgccgttcgc ctggaacggc gtatccctgc acgcgggcgg cgcgacgagc gtacgggtgc 40080ggatcgggca gcgggggcag acggacaccg aaggccgtga gctgaccgtc gtggtcgcgg 40140acccgaccgg cgccccggtg ctcaccgtgg actcggtcgc gctgcggccc gccgacggcg 40200actggctgaa agccgcggaa cggcgttcca cggccgcgct gttcacggtg gaatggacac 40260cgctgccgcc ccaggacagt cggcccgagc cggtcgaggc cgaggacggc tgggcgacac 40320tcggcgcctc cggccccggg caccactacg ccgacctggc cgcgctgctg tcggcggcgg 40380atggcgcaga gcccgcgccg cccgtggtcc tcgcctccgt aactccgacg gccgacaccg 40440gggccgactc cgaggctgac acggatctgg ccaccgtacg gcgcacgttg gggctcatcc 40500aggagtggct ggcggagccg gggctacggg actcccggct ggtgctgatc acctccggcg 40560cgacctcggt cggcgacggc gacgggccgg tggagccggg gagcgctgct gtgttcggcc 40620tggttcaggc ggtccaggcc gaacacccgg accgcttcat gctggtcgac gtgggcgccg 40680acgcggacgc ggacggtgac ggagacggtg gcgagacgtt ggccgatgcc gtacgccggg 40740ccatcgccgc ggacgaaccg cagatcgcgg tccgttcggg cgaggtgtcg gtcccccgcc 40800tgctgcgcgc cgccgcccgg cccgacgagg gcacggcggt cgagctgtcc ggcggtacgg 40860tggtggtgtc gggcgcgatg gaccatgtgt ccggcggcgc gatcgccgag cagctggtac 40920gtgcgtacgg agccgaacgt ctgctgctcc tctcccaccc ggacgagcag gctcccgacc 40980tggccgagcg gctgacggcg ctgggcgcgg cggttgaggt ggcggtggtc gatatcgccg 41040accgcgccgc tctggcggag gttctggcgt ccgtaccgga cagccacccg ctggtgggcg 41100tggtgcatct ggcgggggcg gccgacgagg ggccagtgga gtcgtggaac gacgggcgac 41160tgtcgcgggc gtgggcgccg cgggccaccg gggcgtggca gctgcacacc ctcacccagg 41220acctgccgct gcggatgttc gtggtgtgct ccgccgccgc tgacgtcacg ggcggccccg 41280gccgggccgg atacgccgcc gccaacgccc acaccgacgc cctgatcgcc caccggcgcg 41340ccgccgggct cccgggcacg ggtctggtct gggccctgga ggaagaggcc accgccgacg 41400cctcgcgcct gttcgacgcg gcgttccatg ccgtacagcc gctggtcgtg gccgcggacc 41460tggacaccgc ccggctgggc ccctcggccc ccgcgctgct gcgtgccctg gtccggccgg 41520cccggcgccg cgccgcggaa cggcagtcgg cggcccacgc cctgacctcg cgcctcgccg 41580gtctcgacaa ctccggacag cgcgaactgc tgctggatgt ggtgcgccag atggccgccg 41640tggtcctggg ccactcctcc gacacggcga tccgggccga ggccgccttc aaggagctgg 41700gcttcgactc cctcacggcc gtcgggctgc gcaaccgcct tgtcgacgcc accgggctgc 41760ggctgccctc gaccctggtc ttcgactatc cgaccccccg ggccctggcc gaccacctcc 41820tccagctcgt gaccagcacc gcgcccacca cttcgctccc ggtggggccg gcgcgggccg 41880ccggcgcgga cgacgagccc atcgccgtgg tggcgatggc atgccgcttc cccggcgatg 41940tgaccacgcc cgaggggctg tgggacctgg tcgcggcggg agagaacatc cgcggcccgt 42000tccccaccaa ccgcggctgg gacctggcca acctcttcca ccccgacccc gaacaccccg 42060gcacgaccta cgcctcgcaa ggcgcgttca tctacgacgc cgacggcttc gacgccgcgt 42120tcttcggcat caacccccgc gaggcgctgg ccatcgaccc ccagcagcgc ctgatcctgg 42180aaaccgcctg ggaagccctc gaacgcgccg gtatcgaccc acacaccctc aaggagagcc 42240tgaccggcgt ctataccggc gtcatctacc acgactacgc cgccggtctg cccgcgagcg 42300acccccggct ggacggctac accatgctgt ccagcatcgg cagcatcatc tccggccggg 42360tggcgtacac cctcggcctg cagggcccgg ccgtcaccgt ggacaccgcc tgctcctcct 42420ccctggtggc catgcacctc gccgcccagg cgctccgcca gggcgaatgc gatctggcgc 42480tcgcgggcgg cgtgaccgtc atggccaccc ccgacccgtt caccgggttc tcgcgccagc 42540gcggactggc cccggacggg cggtgcaagc cgttcgccgc cgccgccgac ggcaccagcc 42600tgagcgaggg agccgggctc gtcgtgctcg aacggctctc cgacgcccgc cgcaacggcc 42660atcaggtgct cgccgtactg cgcggatccg cgatcaacca ggacggcgcg agcaacgggc 42720tgaccgcccc caacggcccc tcccagcaac gcgtcatcgg ccaggcactc gccaacgccg 42780gcctcggccc cgccgacatc gacgccgtcg aggcccatgg caccggcacg acgctgggcg 42840accccatcga ggcccaggcc ctgctggcca cctacggcca acaccgcgcc gacgaccggc 42900cgctgtggct cggctcggtc aagtccaaca tcggccacac ccaggccgcc agcggggtcg 42960tcggcgtgat caagatgatc atggcgatgc gccacggccg gctgcccgcc agcctccata 43020tcgacgaacc cagcccgcat atcgactgga ccagcggaaa cgtccagctc ctcaccgaag 43080ccatcgactg gcccgaggcc gaccggcccc gccgcgccgg tgtgtcctcc ttcggcgcct 43140ccggtaccaa cgctcatgtg atcctggaag aggcgccccc accgcccgat cccgcgcccg 43200aaccggccgc cgcgcccgcc atcgcgggcg gcgtggtgcc ctggccgctg tcggcgcggg 43260acgagcaggc gctgcgcgag caggcctcgg cgctcgccga gcacctgggg accgacgacc 43320gtgcgtctgt cgcggacgtg ggatggtcct tggccacgac acgggcgatg ttcgagcggc 43380gggccgtgat cgtcggggag ggccgcgagg agatggccgc cgccctggag gcgctggccg 43440acgggtcccc ccaccccggc ctgtccaccc tcggtggcac cgcttcggac acaccaggga 43500agacggtgtg gctgttctcc ggacagggca gccagcgccc cggcatgggc gccgacctct 43560accggcgttt ccccgtcttc gccgaggcct tcgacggggt ccgcgcgctc ctggaccccc 43620acctcgatca cccgctcgcc gacgtggtct tcgccaccga ccccggacac ggcgacctga 43680tccaccacac cacctacacc caggccgggc tcttcgccct ccacatcgcc ctcgcccgcc 43740tgctgggcga catgggcctg gcgcccgacg cggtcgccgg acactccatc ggtgagatca 43800gcgccgccca cctcgccggt gtgctctccc tggaggacgc cgcccaactc gtcgccgccc 43860gcgccaccct catgggcggc ctcccgtccg gcggcgccat ggccaccgtg aacgccgacg 43920aacaggagat caccgccacc ctggccgact atcccgacct ggccatcgcc gcgatgaaca 43980cccccgcaca caccgtcgtc tccggccccg ccgaccaggt cgccgccctc accgccgcct 44040ggcgggagcg aggccgcaag acccgcgccc tcccggtctc ccacgccttc cactcgcccc 44100agatggagcc catcctcgtc cccttcaccg aggccatcgg ccacctggcc ttccacccgc 44160cccgcatccc gctcatcagc aacctcaccg gcgagcccgc gggcgaggac atcgccacac 44220ccgattactg ggcccgccac atccgcaggc ccgtccactt ccaccagagc atcacccacc 44280tcgccgagga cacggccgtc ttcctggagc tgggccccgc tccggtactg acccacgccg 44340tccaccacac cctccccgag gagaccacag ccaccgcgct cgccaccctc accggtaagc 44400aacccgacgt gcccgccctg gcccacagcc tggcggcgct gcacaccagt cacgccccgg 44460tggactggac gccctggttc cgcaccgatc cggcccccag gaccgtgggc ctgcccacct 44520accgcttcca gcgccggccc tactggatcg ccccccgcgt ctccggcggc gccacccccg 44580gcggtaccgg cctggaccac ccgctgctgg acaccgcggc ggctctggcg gacggcggca 44640tggtgctcac cgggagcgtc cctccggccg accacgacag ctggctcacc gagcgcgcca 44700tcgcgggaac cgtcgtgctg cccggcaccg ccctcctcga actcgccctc cgctgcgccg 44760aggacacccg cagcccccac gtcgaggagc ttctcctcca ccaccccctc accctccacc 44820ccaccgcaca tctcgatctg caggtcgtca tcggcgccgc cgatgacgac gcccgtcgca 44880ccctccacct gtacacccgc ccccagtccg actcctcggc cgagtggacc cggcacgcca 44940ccgccaccct gaccggcgaa cccactgacg accggccgcc ggcagaaggg gaagcggcct 45000ggccgcctgc cggcgccgag cccgtcgacc tcaccggctt ctatgaccgc gccgcgtcga 45060acggctatgc gtacgggccc tccctccgag ggcttcaggc gctgtggcgc cacggcgaag 45120acctcctcgc cgacatcgcc ctgcccatgg ccgacgactc caccgacacc ctcgtcctcc 45180accccgcact cctggacagc gcgctccacc ccctcctcgc cgtcatggac acctcgggcg 45240accaggtctg gctgccgttc tcctggagcg gcgtcaccct gcacgccacc ggcgccaccc 45300acgcccgtgt ccgcgtcacc ccccacgacg accacgagca ccgcatcgct ctcaccgaca 45360ccgccggccg accgatcctc accgccaacg ccgtcgccgt ccggccgacc cgcctcgagg 45420cgcctcaaca gccgctgtcc gaagggctgt tcagcctgga gtggacgccc gtgtcgacgc 45480tggccgaccg gtccgacgcg gccgcgccga cgccgggggt ggtcctcgcc aaggcgccgg 45540tcgcggaggg ggaaggcggt gagctggagg ccgtccagcg ggcgttgacc ctggtacagg 45600actggctggc cgagccgcgg cccgacgatg cccggctggt ggtgatgacc cgcgacgccg 45660tcgccgtgga cggggaagcg cacatcgatc ccgtagcggc ggccgtctgg ggcctgatcc 45720gcagtgctca gacggagaac cccggccggt tcgtcctgct ggacagggac ctggacacag 45780agctggacac ggatccggtc ctgggtcccg atgcccttgc cgaggcggac ggccgggtgg 45840ccgaagcggt ccgctgcgcg ctggacctcg acgagtcgca ggtggcgctc cgcggcggcc 45900gggttctggt accccggctc gtccgcgcga ccgcctcggc cacgctgcct ggccccgtcg 45960accggcggaa ctggcggctg gaggccgcga ccccggccgg cgccgcgtcg ctcgacgccg 46020tggcgccggt gccgttcccc gaggcggagg aggagccggc ggccgggcgc gtacgcatcg 46080aggtgcgcgc ggccggcgtc accttccgcg atgtcctgat cgccaccggc ggggtgcccg 46140acgagacccg cctcggcggc gaaggcgcgg gcgtcgtcct cgaggtgggc cccgacgtca 46200ccgacgtggc accgggcgac cgtgtcatgg gcgtcttcga cggcgcgttc ggccgggtcg 46260ccgacgccga cgcgcgcatg gtgacccgga tgccgaggac ctgggacttc acccgggcgg 46320cgggcgtccc cgtggcgttt ctcaccgcct ggtacggcct ggtggagctc gcggacctca 46380gggcgggcga gtcggtcctc atccacgcgg ccaccggcgg cgtcggcacg gcggcggtgc 46440agatcgcccg ccacctgggc gccgatgcct acgccaccgc cgaccctgcc gagcaccatg 46500tactggaggc gatgggcatc gacgaggccc accgcgcatc ctcccgcgac ctggacttcg 46560agaacgcctt ccgcgccgcc accggcggcc gcggcgtcga cgtggtcctc aacagcctca 46620ccggcgacca catcgacgac cgcaccgacg cgtccctgcg cctgctggcc gagggcggcc 46680gcttccttga tcccggccgg gccgacgcac gcgatcccga acagctggcc aaggacttcc 46740ctgccgtgga ctaccgcgtc tacgacctgg tcccggacgc cgggccggag cgcgtccagc 46800cgatgctggc ggcgctggtg gcgctgttcg acgagggtgt cctggcaccg ctcccggtgc 46860gggcgtggcc gctggccagg gcccgtcagg cgctgcgcca catgagccgg gccgagcaca 46920ccggcaagct cgtgctgacc gtccctcccg ccctcgatcc cgacggcacc gttctgatca 46980ccggtggcac cggcgtgctc gccggcctgg tcgccgaaca cctcgtcacc acccaccaca 47040tcacccatct ccacctcctc agccgtcgcg gacccgacgc ccccggcgcc accgatctgg 47100cgacccgcct cgccgaactg ggtgccaccg tccacatcac cgccgcggac gcctcggatc 47160cgggggccct gcgccgggtc gtcgacgcca tcgatccgga ccatcccctc accggcgtcg 47220tccacaccgc gggcatcgtc gaggacgccg tggtcacctc gcagaccccc gacaccctcc 47280ggcgcgtctg gaccgccaag gcgacgagcg ccgccaacct ccaccaggcc accaagcacc 47340tccccctggc catgttcacc ctgtactcct ccgtctccgg caccctcggc aaccccggac 47400aggccaacta cgccgccgcc aacgcctact gcgacgccct cgccgcccag cggcagcacg 47460ccggcctgcc ggccacctcc atcgcctggg gcctgtggtc caccgccagc gacatcaccg 47520ggcaactcag ccaagccgat gtggcacgga tgggccgcgc cggcgtcagg gcgctggcca 47580ccgaacacgc gctggcgctg ttcgacgcgg cacaccggca aggcgacccg caactcgtgg 47640cgctcaatct cgacgtgccc gccctggccg cgcagccggt cgccatcctc ccggccgcac 47700tgcgcggcct ggccacccgc tcgggcggga ccacccggcg cgccgccgcg gccgtgcagc 47760gccccgacga ctggacgcgc cggctcgccg ggctcccgga ggccgaacag cggcagcagc 47820tgctcacgct ggtacgcggc aacgccgcga ccgtgctcgg ccacgcggat tccgagcgcg 47880tccgggagga ggccccgttc aaggatctcg gcttcgactc cctgaccggc gtcgagctgc 47940gcaaccggct ctcggccgcg accgggctgc ggctgcccgc cgcgctcgtc ttcgacttcc 48000cgtcggcgaa gtcgctggcc gattacctgc gcggccggct ggtggcggac ggggggtcgg 48060cggcccaggc cggtgtcgac ccggtgctcg gcgagctggc gaggctggag tccacgctgt 48120cggccctcga tctgcccgag gccgacgcgc gggcggtgac agatcggctt gagggtctgc 48180tggcccaatg gaaggcggcg tccgcgccac cggccgagga caacgcggcc gaccggctca 48240cgctcgccac cgccgacgag gtgctcgcgt tcatcgacaa cgaactcggc acctcgtgat 48300ggctcatctg ctcgcttcga cgcgtatgaa ggtcgtgact acacatgccg gatgaagaga 48360ggcttgtcga ctacctcaag cgtgtggcga cggacctgca cgacacgcgg cggcgcctgc 48420gcgaggtcga ggagcggcac caggagccca tcgccatcac cgcgatgacc tgccggttcc 48480ccggcggggt ggattcgccc gaggcgttgt gggacctggt ggcctcgggg ggcgacgtga 48540tcgggccgtt ccccgcggac cgcggctggg atctggaggg cctgtaccac ccggaccccg 48600accaccccgg cacgacgtac acgcgcgagg gcgggttcct gcgcgacgcc gacacgttcg 48660actcgggttt cttcgagatc agcccccggg aggcgctggt catggacccg cagcagcgca 48720agctgctcga ggtgacctgg gagttgttcg agcgcgccgg cctcgacgcg acgagcctgc 48780ggggaagtcg gaccggcgtc ttcatcggcg cggcgaccat gggctcgggc acgccgagcg 48840gccccgcccg caaggagtcc gaggggtatg tgggcgtcgc gcccagcatg ctgtcgggcc 48900gcctgtcgta caccttcggc ctcgagggcc cctcgctgac ggtggagacg gcctgctccg 48960cgtcgctggt ggcgatgcat caggggattc acgcgctgcg gcagggcgag tgcggcctgg 49020cggtggtcgg cggggtgacc atcatgtcct cgcccgcggt gttcatcggc ttcgcccgcc 49080agcgcggcct ggcgccgaac ggacgctgca agccgttcgc ggccggcgcc gacggcaccg 49140gctggggcga gggggccggg ctcgtccttc tcgagcggct gtccgacgcc cgccgcaacg 49200gccaccaggt gctcgccgtc atccggggct cggccgtcaa ccaggacggc gcgagcaatg 49260gcttctccgc gcccaacggg ccttcgcagc agcgcgtcat ccgtcaggcg ctcctcaacg 49320cccgcctgtc gtccgccgag gtggacgcgg tggaggcgca tggcacgggc acccggctcg 49380gcgacccgat cgaggccgac gcgctgcacg cgacgtacgg gcagcggcgg ccggccgacc 49440ggccgctgct gctgggctcg gtcaagtcga acatcggtca cccccaggcc gccgccggag 49500tggcgggcgt gatcaagacg gtgatggcga tccggcacgg gctgttcccc gcgacgttgc 49560acatcgacga gccgacgccg cacgtggact ggggctcggg ggccatacgt ctggtgaccg 49620agccggtcga atggcccgag acggaccacc cgcgccgggc cggggtgtcc tcgttcggcg 49680tctccggaac caacgcgcat gtgatcatcg agcaggcgcc cgacccggac acgagcgaga 49740cggacgcggg cgagacggac gcgggcgaga cggcccggga cgcggagggg gcggcgccgg 49800cgcggcaggc cgtggtggcg ggcggcgtgg tgccgtggat gctgtccgcg cgggacgagg 49860cggcgctggc gcggcaggcg ctgcgtctcg ccgaggtggc ggagggcgac ccggccgccg 49920acgtgacgga catgggctgg tcgctggcga cgacacgtgc ccggttcgag caccgggccg 49980tggtcgttgg cacggaccgt gcgacgctgc tcgacggtct ggccaagctg gccgccgacg 50040agcccgaccc cgcggtcgtc accgcgacgg cgggcccgat cggcgccggc ccggtgttcg 50100tcttccccgg ccagggagcc cagtggccgg gcatggcccg tgaactcctc gactcctcac 50160cggtgttcgc cgcccggatc gccgagtgcg agcgggctct ggggccgtac gtggactggt 50220cgctgacgga ggtgctgcgc ggcacggatc cggcgacgga ccccggccgg gacgacgtca 50280tccagcccgt gctgtgggcc atccacgtct cgctggcagc cgtctggcag agcttcggca 50340tcaccccggc ggccgtcgtc gggcactccc agggcgaggt cgccgcggcg tgcgtcgccg 50400gagccctcac cctcgacgac gccgccaagg tcatcgccct gcgcgtccag gcgcttcgcc 50460cgctgatcgg gcacggtgcc atggcctccc tcagcctggg cgccgaggac accgcgcggc 50520tcctcgccga gctcggcgcc gcggccgggg acgtcgccgt cgccgccgtc aacggcccgc 50580acgccacggt cgtctcgggc tcccccgacc atctggacgc cgttctcgag acggcccggg 50640agcgtggcgc acgcacccgc accatcgacg tcgagtacgc ctcccacggc ccccatgtgg 50700accgcatccg cgacgacatc gtctccgccc tgcgtgatgt gacaccggtc gagtccgaga 50760tcgcgttcta ctccaccgtc accgcggagc ggctgaacac cgctgaactc ggcaccgagt 50820actggttcga caacctccgc cgaccggtcc gcttcgcgga tgccgtgggc cgtctgctgg 50880ccgacgggta ccgggccttc atccagtgca acccgcaccc gatcctgtcc acgagcctgc 50940aggacatctt cgaggagtcc ggcacccgcg ccgcctccct cgccaccctc cgccgcgacc 51000acggcggcgc gcatcagctc gccctcgcgc tcgcccaggc ccacgccgcg ggggtcgagg 51060tcgactggcg cccctggttc ccggccgacc gcacgccccg taccgtcgag ctgcccacgt 51120accccttcca gggaaagcgt tactggatac ccgtcggcgg cagcggggcg ggggacgtgt 51180ccgccgccgg tctgcgcgct gtcgaccatc cgctgctggc cgccgcggtg agcctgcccg 51240acggcggcat ggtgctgacc ggacgcctct cggccacaac gggcgccggc tggctcgccg 51300accacgtcgt cggcgacacc acgctgctgc ccggggcggc catggtcgag tgggcgttgc 51360aggccgctca cgaggcgggc tgtgccgcgg tcgaggagct ggccctgcag accccgttcg 51420tgctgcccgc ctcgggggcc ctgcgcgtac gcgtcgcggt ggggcccgcc gacgacgagg 51480gcaggcggac ggtggacgtg tactcgcgcc ccgacgagct cgacaccgag acccccgacg 51540gatgggtgtg ccacgccatg ggcgtgctgg cccccgaggc ccccgaggac cggaccgccc 51600cgccggacgc cccggcggcc ccctggccac cgcggggggc ggaaccgctg gacgtcacgg 51660acttctacga acgggccgcg gccggaggct acggctatgg ccccgcgttc cgcggcctca 51720ccgcggcgtg gcgggacggc gccgatctgc tcgccgagat cgcgcttccc gaggcggccg 51780gcgagggcgc cgaccggttc ggcatccatc cggcgctgtt ggacgccgcc acgcacccga 51840cgatcctcgg cggcggccgg gaggacggct ctgacgccgg gcaggtgtgg ctgccgttcg 51900cgtggagcgg tgtgtcgctg tgggccacgg gcgcccgccg ggttcgcgtg cgcatattcc 51960cggaggacaa cgggcagcgg atctccctga ccgacgagac cggcgcgccg gtcctcgagg 52020ccgcgtcggt cgccgcgcgc cccaccggcc tcgccgagct gcgtgccctc ggcgcgcgcg 52080ccgccgaggg cctgttcgtc gtggactggg tgcccgcgcg aggcgggacg ggtgacgcac 52140cgcccccgga tgacggcggt tgggccacgg tgggcggcgg tggcgtacgg ctcgccgggg 52200tggcggacca cgccgacctg ggcgcgctgc tcgccgcggt ggacgacggg gccccggtgc 52260cgacggtcgt cctccacccg gtgcccgcga cggccacgcc cgacgacggc ctcgccgccg 52320tcgggggcgt actggccctg atacgggaat ggctggccga accgcggtgg ctggactccc 52380ggttggtact ggtcacctcc gacgcggtgt cggccggtga cgacgagggt gcggtggacc 52440cgggtggcgc ggccgtctgg ggtctggtcc gttccgtcca ggccgagcac ccgggccggt 52500tcaccctgct cgacgtgggc ggcgacacgg atgccgacgc gggcgggggc gagagcctcg 52560ccgaggcggt gcgccggtcc atcgacgcgg acgaaccgca ggtcgtcgtg cgcgcggccg 52620gaacgctggt gccgcgtctg gtgcgcaccg ccccggcggc cgaggccgac accccggagc 52680tgtccggtgg cacggtgctg gtgtcgggcg gaacgggtgt gctgggcgga gcggccgccg 52740aacacctggt ccgcgcgcac ggcgtggagc gtgtgctgct cctctcccgt cgcggcccga 52800acgcccccga agcggccgaa ctggtgcggc ggctcaccgc cctgggggcc caggtggacg 52860tggcggcggt tgacgtggcc gaccgcgccg cgctggcgga gacgttgcgg acgatccccg 52920acagccaccc gctgctcggc gtggtccact ccgccggggt gaccgacgac gccctggtgg 52980agtcctggga cgccgaccgg ctcacccggg tgtgggagcc gaaagccaca ggcgcgtggc 53040atctgcacac cctcacccgc gatctgcccc tgcggatgtt cgtggtgttc tcctccgccg 53100ccggcgtcgt cggcaactca ggccaggccg gctacgccgc cgccaacgcc tgcaccgacg 53160ccctgatcgc ccaccggcgc gccgccgggc tgcccgggac ctcggtcgcc tggacgctgt 53220gggaacaggc ctccgccatg accgaacacc tcaccgaggc cgacctctcc cgcctcggca 53280ccctcggcat gcgccccctg gcgacgtctc gtgccctcgg gctcctcgac gcggcgttgc 53340acgtcaccca ccccgtggtg gtggccgccg acctcgacgc cacccgactg ggcccggaca 53400gccccgccat gctgcgcgcc ctggccaggc ctgctcgccg ccgcgccatg gaacaccatg 53460ccaccggccc tgcgctggcg ggccggctgg ccggtctcga cgccaccgcc cggcgcgatc 53520tgctgctgca gacggtccgc cagatggtca cggtggtgct ggggcactcc tccgacgcgg 53580cgatccgggc cgaggccgcg ttcaaggagc tgggcttcga ctcgctgacc gccgtcgaac 53640tgcgcaaccg cctggccggt gccaccgggc tgcggctgcc cgcgaccctc gtcttcgact 53700accccactcc cctggccctc gccgaccacc tcctggaacg cctgaccgcc accgcctccc 53760ccgcgtcccc ccgggccgtt ccgtccaggg ccggggccgc cgacgagccg atcgcggtgg 53820tgtcgatggc ctgccgcttc cccggcgggg tgaccacgcc agaggagctg tgggacctgg 53880tcgcggcgga tcggcatgtg ctcggcccgt tccccaccaa ccgcggctgg gacctggcca 53940acctgttcca ccccgacccc gaccaccccg gcaccaccta cgcctccgag ggcgcgttca 54000tgtacgacgc cgacggcttc gacgccgcgt tcttcggcat caacccccgc gaggccctgg 54060cgatggaccc gcagcagcgc gtcctgctgg aaacgtcctg ggaactgctg gaacgggccg 54120gcatcgaccc ccacaccctc aaggacagtc tgaccggcgt ctacgccggc gtcatgtacc 54180acgactacgg caacggcctg ccccccggcg acccccgact ggacggctac gccgggctct 54240ccgggaccag cagcatcatc gccggccggg tggcgtacac cctcggcctc cagggccccg 54300ccgtcaccgt cgacaccgcc tgctcctcct ccctggtcac catgcatctg gccgcccagg 54360ccctgcggca gggcgagtgc gacctggcgc tggccggggg cgtgaccgtc ctggccaccc 54420ccgacgtgtt caccgggttc tcccggcagc ggggtctggc gccggacggc cggtgcaagc 54480cgttcgccgc cgcagccgac ggcaccggct tcggcgaggg cgtcggcctg gtgctgctgg 54540agcggctgtc ggacgcgcac cgcaacgggc accgggtgct cgccgtcctg cgcggttcgg 54600cggtcaacca ggacggcgcg agcaacggcc tgaccgcgcc caacgggccc gcccagcaac 54660gcgtcatccg ccaggccctg gccggagcgg aactcgaccc ggcggatgtg gacgccgtgg 54720aagcccatgg cacgggcacg acgctgggcg accccatcga ggcacaggcg ctcctggcca 54780cctacggcca ggaccgtccg acggaccggc cgctgtggct cgggtccatc aagtccaaca 54840tcggccacac ccaggccgcc gccggcgtcg ccggggtcat caagatgatc atggccatgg 54900accacggccg gctgcccacc agcctccaca tcgacgaacc cagcccgcat atcgactgga 54960ccaccggaaa cgtccgactc ctcaccgaac ccgccgactg gcccgcgacc gaccgccccc 55020ggcgcgccgc cgtgtcctcc ttcggtgcct ccggcaccaa cgcgcacctc atcctcgaac 55080aggccccgga ccgccccgga gacgggccgg cgggggaccg ccccgcgccc gtggccgtgg 55140cctggccgct ctccgcccgc accgacgagg ccctgcggac cgtggccacg gccctcgccg 55200accggctggg agccgacgac accacgccgg tcacggacgt ggggtggtcg ttggccacgg 55260cccgggcgac attcgaacga cgagccgtga tcatcggaag cgaccgccag gagatgaccg 55320ccgccctgga cgccctggcc cgggacctcc ctcacccgaa cctggtcgcc ccgctccccg 55380tcgcgccccc ggcgggcgac acggtgtggc tgttctccgg gcaggggagc cagcggccgg 55440gcatgggcgc cgaactccat gagcgtttcc ccgccttcgc cgacaccttc gacgagatct 55500gcgcactgat cgacccccac ctcgaccacc ccctgcgcga catcgtcttc gccacccacc 55560ccgaccacac cgacctcctg aaccacacca cctacaccca agccggactc ttcgccgtac 55620aagtggcact cgcccggctc ctggaacact gcggcctacg acccgacacc gtgatcggcc 55680actccatcgg cgagatcacc gccgcccaca tcgccggcgt cctctccctc caagacgcct 55740gccacctcgt cgccaaccgc gccaccctcc tcggcaaact cccacccggc ggcgccatga 55800ccgccatcga agccaccgcc gaagaaatca cccaaaccct caccccctac cacggccaag 55860tcaccatcgc cgccctcaac gcccccacca gcaccgtcat ctcaggaccc gaagaactcg 55920tggcccaact cacccgcaga tggaaagaac gcggccgcag aaccaaaaca ctcaccgtca 55980gccacgcctt ccactccccc ctcatggaac ccgccctcaa cgacttccgc cacgccatcg 56040accacctgac ctaccaccag cccaccatcc ccctcatcag caacctcacc ggcgaacccg 56100caacccaaga catcgccacc cccgactact gggtgcgcca catccgccaa cccgtccact 56160tccaccccgc catcacccac atcgcacccc acacagcggc cttcctcgaa atcggccccg 56220acgccacact catccccgcc acacagaaca ccctcgacac actggagaac caacccacct 56280ccgcacccca actgatcccc accctcaccc gcaaacaacc cgacacccaa gccctcgccc 56340acgccctcgc ccgcctccac accctcaccc ccctcaactg gcacccctgg tacaccgacc 56400aacccacccc caccaccatc gacctcccca cctacccctt ccaacacgag cgctactggc 56460tcactcccac ccacgccggg cccaccaccc cgggcgccac cccgctcacc caccccttcc 56520tcgccgccac cgcaccgctc gcggacggtg gcctcctgct gaccgggcag gtcccctccg 56580ccgaccacgc gggctggcac accgagcaca ccatcgcggg cgccaccctc ctgcccgcca 56640ccgccctcct cgagatcgcc ctccacgccg ccgaccacac caccaccccc cacatcgacg 56700aactgatcct gcagcacccg ctcaccctcg accccagcca ccccctcgcg ctccaagcca 56760tcgtcagccc tgctgatgac tccggtcacc gcgccctcca catctacaca cgcgcgccgt 56820ccagccccac cgcggagtgg acccaccacg ccaccgccac cctcggcgga gagcccacgg 56880ccgagcggcc cacgaccgaa gcggaggcgg cctggcctcc cccgggagcc aaagccgtcg 56940acatcaccgg cttctacgac cgcgccgccg cggacggcta ccactacgga cccagttacc 57000agggcctcca aaccgtctgg cgccagggcg aggacctcct cgccgacatc actctgccca 57060cggccggcac gcccgaccac accaccgact ccctggccat ccatcccgcc ctcctcgacg 57120ccgcgctcca cccgctcctc gccaccgccg acaacccgga tggcgagatc tggctcccct 57180tcacctggag cggcgtcacc ctccacgcga ccggtgccac ccacgtccgc gcccgcatca 57240ccccccaggg cgacaacgac taccgcctca ccctcaccga cgcgaccggc caaaccgtcc 57300tcaccgccgg caccatcgcc tcccgccccc tcgacaccgc gcggctgcgg acgcgcgggc 57360cgggtgacgg cctgtaccag gtgcggtgga cggcgatgcc gatcccggcc ggatcggcga 57420ctgccgtggc ggacgactgg gcgatgctcg gggatgccgg gctcagggac ggcgggctcg 57480ccgatgcggt cgcgccgctc gcgtcgtatc cggatgtcgc cgcactggtc gcggccatgg 57540acgacggcac gcccgtgccg tcggtcgtcc tgaccggcct ggcacccgcg gacggcggtg 57600acgccgatgt ggtggtggag gtgctgacga cggcgcggga atggctggcc gagccgcggc 57660tcgccgagtc ccggctggtg gtcgtcaccc acgacgccgc cgtcgccgag gacaccgaca 57720gcggcccgga cggcggcgat gtggatccgg tggccgcggg cgtgtggggg ctgatccgca 57780gcgcccagtc ggagaacccc ggccggttca cgctgctcga cctcacccgg cgcgacgccg 57840gtacggcacc ggatgtcgtg gaggtgctgc gcgcggccat ggacgcggac gagtggcagg 57900tggccgtgcg cggcgggcgg gcgctggtac cccggctgac ggccgcggac gcggcggccg 57960gcatcgtact gcccgtcggc gcgcctgcct ggcagctcgt catggcggac gagcgcgcgg 58020gtacggtcga cggcttggcg cccgaggaat gcccggaggt gctggaaccg ctggcgcccg 58080ggcaggtgcg catcgccgtc cgcgccgcgg gcgtcaactt ccgggacgtc atggtgaccc 58140tcggcgtcgt gcccgaccgc cgcggcctgg gcggtgaggg cgcgggcatg gtgctcgacg 58200tggccccgga cgtgacgtcg gtggccgtcg gcgaccgcgt catggggttg ttccagggct 58260ccttcggccc catagccgtc gccgacgccc gcgccctggt gcctgtcccg ccgggctgga 58320ccgaccggca ggcggcggcc gtgcccatcg cgttcctcac cgcctggtac gggctgatcg 58380acctcgccgg cctcaaggcg ggcgagtcgg tcctcatcca cgccgccacc ggcggcgtcg 58440gcacggccgc cgtgcagatc gcccgccacc tgggcgcggt gatctacgcc accgccagcc 58500ccggcaagca cccgatgctg gaggcgatgg gcgtcgacga gacccaccgc gcctcctccc 58560gcgacctgga cttcgagcac atcttccgcg ccgccaccgg gtccgagggc atggacgtgg 58620tgctggactg cctggcgggc gagttcgtgg acgcgtcact gcggctgctg ggtcagggcg 58680gccggttcat cgagatgggc aagaccgaca tccgtgaccc cgagcagatt gccgacacgc 58740accccggcgt ccactaccgt tcgtacgacc tcgtctccga cgcgggtctc gaccgccttt 58800cggagatgct gggtacgctc gccgacctct tcgcccaggg cgtgctcaca ccgcccccgg 58860tccaggcatg gccgctggcc agggcccgcc aggcgctgcg ccacatgagc caggccaaac 58920acaccggcaa gctcgtcctc gacatccctc ccgccctcga tcccgacggc accgtcctga 58980tcaccggcgg caccggcacc ctcggcgccc tcatcgccga acacctcgtc accaaccacc 59040acatcaccca cctgcacctc ctcagccgcc gcggacccga cgcccccggc gccgccgaac 59100tgacggccca cctcaccgaa ctcggcgcca ccgtccacat caccgccacc gacaccaccg 59160acccccacgc cctgcgccag gccctcgaca ccgtcgaccc ccgccacccc ctcaccgccg 59220tcatccacac cgccggcatc gtcgacgacg ccgtgatcac cgcccagacc gccgacagcc 59280tccaccgcgt ctgggccgcc aaggcgacga gcgccgccaa cctccaccag gccaccgagc 59340acctccccct ggccatgttc gtgatcttct cctccgccgc cggtacgttc ggcagccccg 59400ggcaggccaa ctacgccgcc gccaacgcct actgcgacgc gctcgccacc cggcgccggc 59460acgccggcct gccggccacc tccatcgcct ggggcctgtg ggcggccacc agcggcatga 59520ccggcggact caccgagatc gaccacgcga ggatgagccg gtcgggcatg gcaccgctgc 59580cctcggagca cgccctggcg ctgttcgacg cggcgcacgg gctcggtgcg gcacgggtgc 59640tcgccgcccg gctcgacctg gcgaggctgt ccgcccagcc gaccgaagcg ctgccgccgt 59700tggtccgctc gctcacgggc accggccccc ggaccgcgcg gcgcagcgcg gcggctcccg 59760tggccgacct gtccggccgg ctggcgtcga tggcccccgc cggacagctc gcgctcctgc 59820tggatctggt ccggacccac gcggccaccg tgctggggca catggattcc ggcacggtgt 59880cggcggacac ccccttcaag gatctcgggt tcgactcgct gacggcggtc gagctgcgca 59940accggctcac cacggtgacg gggctgcggt tgtccgcggc gtcggtgttc cgctacccga 60000cagccaccgc catggccgag cacctgcggg gcgagctgtg cccgacgggg gatgacacgg 60060cgcagcccgt gctgcgggag ctggcacggc tcgaggcggc ggtgggcgag tcgaagccgg 60120agggggagac cagtgcccag ctcgtcaagc ggttgcagac cctcttgtgg cggctcggtg 60180acgaggccgc cgcggtcgat cacaccgtcg acggcgagga gttggagtcc gcctcggacg 60240acgagatgtt cgcgctcatc gaccagcaac tgggctcgtc ctgaccgtca gcgagggaga 60300agtcgtgtca accactgaag agaaactgcg ccagtacctc aagcgcgtca ccctcgacct 60360cggccaggcc aagcaacgcc tgcgggaagc ggaagaacgc caccaggaac ccatcgccat 60420caccgccatg gcctgccgct accccggcgg cgtcaggtca ccggaagccc tgtgggacct 60480cgtcgccacc cgcaccgacg ccatcggccc tttcccgacc aaccgcggct gggacctgga 60540gggcctgttt cacccggacc ccgaccacta cggcaccagc tacgtccggg aaggcggctt 60600cctccacgac gccgagcggt tcgacgcctc cttcttcaac atcagccccc gcgaagccct 60660cgccatggac ccccagcagc gagtgctcct ggagaccgcc tgggaactgc tggaacgcgc 60720ccacatcgac ccccacagcc tcaaaggcac cctcaccggc gtctacacgg gggtgtcgag 60780ccaggactat ctgtcgcgga taccgcggat tcccgagggt ttcgagggct atacggccac 60840gggcggactc atgagcgtgg tgtccggccg cgtggcgtac acgctgggcc tggagggacc 60900ggcggtcacg ctggacaccg cgtgctccgc gtcgctggtg gcgatgcacc tcgccgggca 60960ggcgctgcgg cagggcgagt gcgacctggc cctggccggt ggtgtgaccg tgttcagcac 61020gcccaccgcg tatgtggagt tctcccggca gcgggggttc gcaccggacg cccgctgcaa 61080gccgttcgcc gccgcagccg acggcaccgg cttctccgag ggcgtgggcc tggtgctgct 61140ggagcggctg tcggacgccc agcgccacgg gcgtcgtgtc ctcgccgtgc tgcgtggctc 61200ggcggtcaac caggacggcg cgagcaatgg gctgtccgcg cccaacgacg ccgcgcagga 61260gcgcgtgatc cggcaggcgc tggacagcgc ccggctcacc gccgaccagg tggacgcggt 61320cgaggcgcac ggcaccggaa ccacgctcgg tgaccccatc gaggcgcagg cgctccttgc 61380cacctacggc aaggagcgtt cggcggaccg gccgctgtgg ctggggtcgg tgaaatcgaa 61440catcggccac acccacgcgg cggcgggcgt ggcgggcgtg atcaagatgg tgatggccat 61500gcatcacggc cggctgcccg ccaccctgca cgtcgacgag ccgacctccc atgtggactg 61560ggacacgggc acggtgcgac tgctgaccga gccggtcgac tggccgcggg gggaccgtcc 61620gcgccgggca ggcgtgtcct cgttcggcat ctccggtacc aacgcccatg tgattctgga 61680ggaggccgcc ctgccgcccg ccgccacggg cgccgagcgg ccgggagacc ggctcacgcc 61740gtgggtggtg tcggcgcgcg gccaggccgc gctgcacgac caggcccggc ggctgctcga 61800cgcgaccgtt gacggcgatc ccgaggcagt gggctggtcg ctggtcgcct cgcgtgcggt 61860gttcgaccag cgggcggtga tcacgggccg ggacaccgaa acgctgcggg cgggtctggc 61920cgcactggcg gccggggagg accatccggc gctggtgcga cgtgaggcgg gggtaccggc 61980ctcggggtcg caggtgtggc tgttctccgg gcaggggagc cagcggccgg gcatgggcgc 62040cgaactccat gagcgtttcc ccgccttcgc cgacaccttc gacgagatct gcgcactgat 62100cgacccccac ctcgaccacc ccctgcgcga catcgtcttc gccacccacc ccgaccacac 62160cgacctcctg aaccacacca cctacaccca agccggactc ttcgccgtac aagtggcact 62220cgcccggctc ctggaacact gcggcctacg acccgacacc gtgatcggcc actccatcgg 62280cgagatcacc gccgcccaca tcgccggcgt cctctccctc caagacgcct gccacctcgt 62340cgccaaccgc gccaccctcc tcggcaaact cccacccggc ggcgccatga ccgccatcga 62400agccaccgcc gaagaaatca cccaaaccct caccccctac cacggccaag tcaccatcgc 62460cgccctcaac gcccccacca gcaccgtcat ctcaggaccc gaagaactcg tggcccaact 62520cacccgcaga tggaaagaac gcggccgcag aaccaaaaca ctcaccgtca gccacgcctt 62580ccactccccc ctcatggaac ccgccctcaa cgacttccgc cacgccatcg accacctgac 62640ctaccaccag cccaccatcc ccctcatcag caacctcacc ggcgaacccg caacccaaga 62700catcgccacc cccgactact gggtgcgcca catccgccaa cccgtccact tccaccccgc 62760catcacccac atcgcacccc acacagccgt cttcctcgaa atcggccccg acgccacact 62820catccccgcc acccaaaaca ccctcgacac cctcgacaaa caacccgcac acccacccca 62880actgatcccc accctcaccc gcaaacaacc cgacacccaa gccctcgccc acgccctcgc 62940ccgcctccac accctcaccc ccctcaactg gcacccctgg tacaccgacc aacccacccc 63000caccaccatc gacctcccca cctacccctt ccaacgcgag cggtactggc tgcccgatgc 63060cctcgcggac gccccgccgc cggaggcgga cgaggagcag gtccggttct ggaacgcggt 63120ggaggcgcag gatctcccgg ctctgtccga cacgctgggc atcggcgagg aggacgggcg 63180gcgctcgtcg ctcggcgcgg tgctgccgac gctgtcgcgc tggcaccagg aacgccatga 63240gcgggcgacc gtgagctcct ggcgctatcg ggtgggctgg cgccacctgc cggacctcgg 63300cccggcggcc gtggcggggc cgtggctgct ggtcgtgccg ccaaagggcg ccgacgcgtg 63360ggccgacgcc tgtgagcggg ccctcactgc ggacggcggc gaggtgcggc ggctggtgac 63420ggacgggcgg gccgatgtgg ccgagctcgc cgcgtcgctg cgggcgctgt acgccgaggg 63480cccgtccccg gcgggggtgc tgtccctgct gcccctcgac gagcgtccgc acgaggcgtt 63540ccccgccgtg accggcggtg tgacgggcac ccacgtcctg ctgcgggccc tgctggacgc 63600cgagctcgac gcgccgctgt ggtgcgccac gcggggcgcg gtggcggtcg atgacgatga 63660agcacctgag gccccggcgc aggctcaggt gtgggggctg ggccgggtgg ccgcgctgga 63720gcatccgacg gcgtggggcg ggctggtcga cctgccggcc tcggtcgcgg acctcgctcc 63780cgatctgctg tgcgccgtgc tggcggggcg gaacggggag gaccaggtgg cgctgcggcc 63840ggccggggcc ttcgggcggc ggctgctgcc cgctccgctg gatgcccagg ccccggccca 63900ggagcgggcg tggacgcccc gggatggcgt cctcgtcacc ggcggggtcg ccggagccgc 63960ggccctcgtg gcgcggtggc tggccgcgga cggcaccaag cacatcgtgc tgctggcgcc 64020ggacggcccg gccgcgcccg gtggtgcgga actggtggcc gagctggcgg agctgggcgc 64080ggaggcgacg gtggtggacg gcgtgccgtc cgaaccgacg acccgccagg agctggccga 64140ccggctcgcg gcctcgggtc tgcgggtccg cacggtcgtg cacgccgggg cgccgggcga 64200ctgggcgccg ctggcagagc tcaccccaga cgagctggcc gaggcgctgt ccgacgccat 64260ggggggagcc gatcggctcg cggagctgtg cggcctggag cccgacgacc cggtggtggt 64320cttctcgtcc atcgccgcgg tctggggcgg tggcgggcac ggtgcccggg cggcggccga 64380cgcgtatctc gacgcgtggg cgcggcggcg ccaggcggcg ggcggccatg tcgcccggct 64440ggcgtggggc gtgtgggacg gctcggaaga cccggaggcg gccgaacgcg ccgagcgtca 64500ggggcttttg gccctccacc cgacgcccgc gctggccgcg ctgcggcgga cgctcgatca 64560cagcggtgac ggaaccgacc aaggcaccgt ccgggccggt gatgagggcg gcgaccggag 64620tgacgtccac gcggtgatcg cggatgtgca ctgggaccgt ttcgtgcccc tgttcaccat 64680ggcgcgtgcc agccgcctct tcgacgagat cccggcggcc cggcgggcgt ggcaggcggc 64740actggactcc tcggacgacg agagttccga gagcctgacg gccctgaggg accggctggc 64800ggcccagtcg ccccaggcgc gcaccggcac gctgctggcc ctcgtccgcg cccatgtggc 64860cggggcgctg cgctacccgg cggcggagtc ggtcgatccg gagcagccct tcaaggagct 64920gggtttcgac tccctggcgg ccgtcgagtt ccggaaccgg ctacggggcg ccatcggcct 64980gacgctgccc gccaccctgg tcttcgacta tccgacgccg accgcgctgg ccgggtatct 65040ggtctcgcag gtcctgccgg ccgagccggc ggacgaaccc gccgccgcgc atctcgacga 65100gatcgaggcg acgctcgccg ccctggacgc ggacgacccg cgccgcgccg ggctgacgca 65160ccgactgcgg ttgctgctgt ggcggtacgc cgacggggac gatgcgctcg aaccgcgcga 65220ggagacgggc ggggacgacc tggaaacggc gtccgcggac gagatgttcg ccctcatcga 65280ccgcgagttc ggggagtcct gagcacggcc gagcccctga ccccggcggg gtcaggggct 65340cgtggcgatc gtgagccgga gcgatcagcg agaacggagc tcctccgtca gccgttccag 65400ttcggggacc acctcgttgg gggacggctg agccagcacc tcctgacgga gccgctcggc 65460acccgcccgg taggaggggt cttgcaccag cttcgccagg tgattccgga ttcgctttcc 65520tgtcatgcgc tccggcggga tccacccacc cgcctgggcc tcttccagcc gtgcgcctcg 65580caccgaggcg tcgggggcca tacggctgac catgagctgt gggacgccgt gcgccagcga 65640gctgcagaag gcgggaagtc cgccgtggtg aatcaccgcc gcacaggacg gaatgacgag 65700gtgcagcggg acgaaatcga caagcctggt gttctggggg acgtgcttca gtttttcctg 65760gattctcccc ggcagggtca tcaccagttc catctcgaga tcggccatcg agtcgagcca 65820gtcctgcagc tgctcaatgg agaccatctg gtagctggag acgggctgcg agctggacat 65880gtcccacatg ctcatcccga atgtggcgag gacacgcggc gccggcggct cagggcggat 65940ccagtccggc gcgacggcgc ggccgttgta cggcacatgg cggacggaga cctgcggcac 66000attggctttg agcctcatgg agtccggcac ctggtcgatc gtgaactggc cggtcaccag 66060ctcttcgcgg aactcgaaac cgtgtttctc cccccacgcg ccgagccatt cggccatggg 66120gtcctcgcgg tccgccggct cctgctgcgc cagcagcttc aggaagtggc ggcgcatacg 66180ggcctccacc tcgataccga tcggcatccg cgcgtgtgcc gcccccaccg cggcggcggc 66240gaccgccccc gcgtggctca gccactccca gaccaccagg tcgggcttcc accagcggca 66300gtacgcgacc agatcgtcga tcatcgaatc gttcacggcc ttggccactt cggtggaagt 66360ggcgcagagc gacttcaggt agctcagcgg aaacatctct tcgcgattct cgcccagatc 66420gatgggatgg tgaattccac cccatgtcgc ttcggatgct tccgtctgcg cttggtgcgc 66480cttctccagg aagtgctcct ccgatccgac cgggaccgcc gtcagccctg actggacgat 66540gacgtcggtg aggtcggggc cgctggcgac atgaacctcg tggcccgcgg tgcgcagggc 66600ccaggccatc ggcacacaga actggaagtg ggtccgccaa ggaatggtga cgaacaggac 66660acgcatggct ggcatctctc ctctctggtc accggcaatg gcaaccagtt cgctctctcg 66720cccgtgcacc ttcgtcattg accgcactca cgtttaccct aacgacgcct ctaaagaggg 66780tcttgcacgt gctcgaccgg cgttagtccg cctttagccg tgcccgagag actgcgtacc 66840gccgggatgc gaccaacgcc gaacgtatga ggagagcggt atggctcagg gttttcaggc 66900cagcctgcag tgggaacgca tcaatgaact ctgggttacc gaggaggcgt cggctgatct 66960gaccggcttc aagtcggacc gccggaattt caatatcgcc ctgtgggatc cgacgaccaa 67020cggaatcagg tatctgaggg ctctcgtcta cgagttggcc acgcggctga gcgacgacga 67080ctggtcgaag atcgagaaag tccgcaaccg tgacgtggga gatccggtca ccgtccgata 67140cgagggcagg accgtctgtc tggactatct ccaggcggcg ctcgagctcg gcttcatcga 67200gaaggaactg gacctgggcg gcgcccgcgt cctggagatc ggcgccgggt acggccgtac 67260gtgccatgcg atgctctcca actacgacct ggcctcctac accatcgtcg acctgaagaa 67320caccctcggg ctgagcaggg cgtatcttcg cgaggtcctg gacgagaagc agttctccaa 67380gatgaggttc gtccaggtgg aggacatcga cacggggctg ggcccggacg gcttcgacct 67440gtgcgtcaac gtgcactcct tcacggagat gaccccggac accgtcaagg cgtatctgcg 67500cctgatcgac gagaggtgcg gggcgttctt cgtgaagaac ccggtcggaa agttccggga 67560caagagcatg gacggccacc agaagggcga ggaggccgtg cggctggcca tgcagaccgg 67620gccgctgcgg caggtgctcg acatccatga cagccaggcc gtggcggccg ccgtgccggc 67680cttcatcgag gcctatcagc ccggcgaggg atggacgtgc gccgccaaca cccgcggcat 67740gccctggagt tacttctggc aggccctcta cacgaagacc ggcgacgacc ttcgatagcc 67800cccgtcttcc caggaggaaa ccatggccgc ctctccggtg cccccgcccc gcggtgacga 67860agcgctggcc ggcacgccgg tcctggtcct gggcggctcc ggctacctgg ggcggcacat 67920atgctcggcg ttcggtgccg cgggcgccca ggtggtgccg gtctcgcgcg gcgcgcgcgg 67980cggcgtggac ggtgacggct gccgctcggt gcgcctggat ctgaccgcgg ccgggcccga 68040tgagctggcc cggctgtgcg ccggtacagg ggcgcgggtg ctggtgaacg cctcgggcgc 68100ggtctggggc ggtggcgaac ggcagatggc cgaggccaac accgagctgg tcggccggct 68160ggccggggcc gtcgcccgat tgccgggccg gccgcggctg attcacctgg gcagcgccta 68220cgagtacggc ccggcccgcc cggggaccgc gatcgcggag gactggccgc ccgccccgac 68280caccgtctac ggccgcacca agctgagcgg ctcgcaggcc gtccttcggg ccgccgccga 68340gctcggtgtc gccggaaccg tgctgcgggt ctccgtcgcc tgcggcccgg gcgccccggt 68400gagcagcctc gccggggcgg tggccgcgca cttggcggcc ggccgcgacg agttgcggct 68460cgcgcccctg cgcgaccacc gcgacctggt ggacgtccgg gacgtggccg acgcggtggt 68520cgcggcggcc gtggcaccgg tcgccgccgt caccggcaca gtcgtcaaca tcggcagcgg 68580ccaggcggtg cccgtgcgcc ggctggtcga tctgatgatc gccctcagcg ggcgcccggt 68640gcgtgtcatc gaggaccccg cgctgcgccg gacgcgttcc gacgcggcct ggcagcgact 68700ggacatcgga cgtgcgcggc gcctgctggg ctgggcgccg cgccgaaccc tgcgggagtc 68760actgcgcgat ctgctcgccg ccgtcggcgc gccgcagccc gcggcggtac gagcggcgac 68820agcgatcgga ccccggaaca gtcatgggaa ggacagcaga tgagcgaacg ggtcgcgcgc 68880atcctcgacg aggtgcgcaa gtaccaccag gacagccagg aggggcgtgg cttcatcccc 68940ggggtcaccg agatctggcc ctcgggcgcg gtgctcgacg aggacgaccg ggtcgcgctg 69000gtgcaggccg cgctggagat gcggatcgcc gcgggcaagc tctcccggaa gttcgagtcg 69060gccttcgcgc gccggatgaa gcgccgcaag gcacacctga cgaactccgg gtcgtccgcg 69120aacctcctcg cgatctccgc gctgacctcc cacctgctgg gcgagcgccg gctgcgcccg 69180ggcgacgagg tcatcacggt ggcggcgagc ttccccacca ccgtcaaccc gatcctgcag 69240aacgggcttg tcccggtcta tgtggacgtc gagttgggga cgtacaacgc cacggccgag 69300cgggtggcgg aggcgatcgg gccgcgcacc cgggccatca tgatggcgca cacgctgggc 69360aaccccttcc aggccacgga gatggcccgg ctggcgcagg accacgacct gattttgatc 69420gaggacagct gcgacgccgt gggctccacc tatgacgggc ggccggccgg gacgttcggc 69480gatctgacga ccgtcagctt ctaccccgcg caccatctga ccatgggtga gggcggctgc 69540gtgctgacct cgaacctggt cctggcgcgg atcgtggagt cgctgcggga ctggggccgg 69600gactgctggt gcgagccggg cgagagcgac acatgccgca agcggttcgg gtaccagatg 69660ggcacgctgc cggccggcta cgaccacaag tacatcttct cccacatcgg gtacaacctg 69720aagtcgaccg acctccaggc ggcgctcggg ctgacccagc tcgacaagct cgacgcgttc 69780tgctcggccc gtcggagcaa ctggcggcgg ctgcgcgaag ggctggacgg gctgccgtgg 69840ctgatcctgc cggaggcgac gccgcgctcc gatccgagct ggttcgggtt cgtcctgacc 69900gtcgatccgc gggccccgtt cagccgcgcc gagctggtcg acttcctgga gtcccgcaag 69960atcggcacac gtcggctgtt cgccgggaac ctgacgcgcc atcccgcgca tgccgaagcg 70020ccacaccggg tgtgcggcga tctggccaac agcgacaccg tcaccgagca cacgttctgg 70080gtcggcgtct atccagggct gaccgaggag atgatcgact tcatggtctc ctccatcacc 70140gagttcatcg ggagccaccg gtgagcgcgg agcgtttgag cggctcccac caggggcggt 70200tgtcccggta ccaggcgacc gtctcggcca gcccggccgc gaagtcgtgg cgcgggcggt 70260agccgagttc gtccctggcc ttgtcgtcct ggaccgcgta gcgccggtcg tgccccttgc 70320ggtcctcgac gtaccgtacg cggctccagc cggcgccgca ggcgtccaac agccgaccgg 70380tcagctcccg gttggtcaac gcggtgccgc cgccgatgtt gtagatctcg ccgggcgatc 70440ccttggtgcg caccagttcc acgccgcggc agtggtcctc cacgtgcagc cagtcgcgca 70500cgttcagccc gtcgccgtac agcggcacgt cctcgccgtc gagcaggttg gtgatgaaca 70560gcgggatgat cttttccggg aactggtggg gcccgtagtt gttgctgcac cgggtgacgc 70620gtacgtccag cccatgggtg cggtggtagg cgagggcgat caggtcggag gcggccttgg 70680aggcggcgta cggcgagttg gggtccagta cggcggtctc ggggctgaat ccggtctcga 70740ccgagccgta gacctcgtcg gtggagacat ggacgaaacg ctccaccccg tggcgcaggg 70800ccgcctgcag cagcgtgtgg gtgccctcga cattggtgcg cacgaacggg tcggcgccgg 70860tgatggagcg gtcgacgtgg gattcggcgg cgaagtgcac cacttggttg gcccgcgcca 70920tcagcgtgtc gaccaggtcg gcgtcgcaga tgtcgccgtg cacgaacgtc agccgggggt 70980cggcggtgtc gaggttgctc agtgtccccg cgtaggtgag cttgtccagc acggtgacat 71040gtgagacgtc ggggccccac tccgcgccgc cccgtgcgag gagcctgcgt gcataggtgg 71100acccgatgaa ccccgcggca cctgtgacca ggagcttcat atctcctcca gaaagcgcgc 71160gggcggcagg ccccgccggt acgagcgcgc ccggtccatg acgtactggc cgtagggtga 71220gttgctcatc tcggtgccga gggtgtagca ctcgtcggct ccgatgaatc ccatgtacat 71280ggcgatctcc tcgacgcagc cgagccgcgt gccttggtag tgctgaacgt cgcgcaccat 71340acggcccgcg tccaggaggg tctcgtgggt gccggtgtcc agccaggtga cacccctgcc 71400gagccacacc agatcggccg tgccctcctc gagataggcg cgcaggacgt ccgtgatctc 71460cagttcgccc cgggccgagg gcaccaggcg cttggccacg tcgacgacgc cgccgtcgaa 71520gaggtacagg ccgggaatgg ccaggttcga ccggggccgc tcgggcttct cctcgatgga 71580cagcagcctg ccgcgctcgt cgatttcggc gacgccgaag tgccggggat cggcgacctc 71640gtgcccgaac agcacgcatc cgcgcagccg ctgcacactt ctacggagca gcgcgggcag 71700attcgcgccg tggaagaggt tgtcgcccag gatcagcgcg cactcctctc cgcgaatatg 71760atccgcgccg atccggaagg cgtcggcgat tccccgcggt ttctcctggg aggcataaga 71820gaggttcagc cccaggcgcc ggccgtctcc gaaaagctgg cggaattgag gcagctcggt 71880ggggcgactg atgatcagga tatcccggat gcccgcgaac atgagaacgg acagcggata 71940gtagatcatg ggtttgtcgt aaacgggaac caactgtttc gaccccgcca aggtgagggg 72000ctgcagccgt gttccgttcc cgcccgcgag aatgatgccc ttcatcatgt caggacttcc 72060cgaactcggt ggcgatcaaa tcgaatatgt cctccgccgt cgccgtttcc aggtcgctgt 72120gggacccggt ggcgcgggcg gtgtcgctcc acttgtcggc caggagccgc agccgtgcgg 72180cgatccggga gcaggcggcc tcgtcgacgg tgtccggggc gtgggcggcg tcccagccgt 72240ccagttccga catgatcagc tgttcgcccg aggccgcgtc ggcgacgagc agcccgtgca 72300gatggcgggc gagctcctcg gccgaggggt agtcgaacag gacggtggtg ggcagccgca 72360gcccggtgcc ggcgttcagc cggtcccgca gttggacggc ggtgagcgag tcgaagccca 72420gctcctggaa cggcttggtg gggggcacgg cgtccgcgtc ggcgtgaccg agcgtggtgg 72480ccgcgtgcgc ctggacgtgt cgtacgagga tgtggtgctg ctcggccggc gggcagccgg 72540cgagcttccg cctcagcggg tccgcctcgt cctccctggg ccgggtctcc tcggcgcgcg 72600cctcggcggc ctccggaagg tcggagagca gcgggctcgg gcgcagcgcg gtgaagcccg 72660tcaggaaacg ccgccagtcg atggcggcga ccacggcgtt ggtctcccgg cgttccaccg 72720cctgctgaag ggaggcgatg gccaggccgg ggtcgagggg gtggatgccg cggcggcgga 72780aggcgtcgac ctcgtcggcc gtggtggcca tgcccacttc gccccacagc ccccaggcca 72840cggaggtggt cggcaggccg aggccgcggc ggtgctcggc gagggcgtcc aggtaggcgt 72900tggcggcggc gtagccggcc tgctgcccgc tgccccagga cgcggagacc gaggagaaca 72960ggatgaacgc ggagaggtcg aggtgccggg tcagctcgtg gaggtgccag gccgcgagcg 73020ccttcggtgc cagcatgcgg ttcaggtgct cgtcgtccac gtcggcgatg aggtcctgct 73080cgctgacgcc cgcggcgtgg atgacggcgg acagcgggtg ctcggcgggg atcgcgtcca 73140gcacgccgcg catggcctcc gggtccgccg cgtcgcaggc ggcgatggtc accgcgacgc 73200ccaggccgcg cagttcggcg gccagttccg cggcgccggg agcgttgggg ccacggcggc 73260tggtcaggac cagttgggcg gccccgcgtt cggcggccca gcgggccagc agcgctccga 73320cgcctccggt gccgccggtg atcagcacgc tgccccgggg ctgccacgcc tggtccgcgg 73380tacgcgcgct cgtggtgtgg acgaggcggc gggccagcgc cccgccaccg gcccggatcg 73440ccacttggtc ctcggcctgg ccgcccgtcg gcgcgccggc cagcagggcc gccagctgtg 73500cctcggtccg ctcgtcgggc tcggccggca gatccaccag acccccccac cggtcggggt 73560gttccagcgc ggccacccgg cccaaacccc aggtctgggc ctggaggggg tgcgacagcg 73620ggtcgtcgtg gccggtggac accgcgccct gggtcacaca ccacagcggc gcggtgatcc 73680ccgcgtcgcc gagcgcctgg agggccgcca cggtggcggc gagcccggcg ggcacggccg 73740ggtggtcggc gcgggggcgc tcgtcgagcg cgagcaggct gaccaccccg gccggggcgt 73800cgtcaccgcg caagcccgtc agccgttcgg cgagttcggc gcggctggtg acggaggggt 73860cggcggggca gggccggacc tcggcgccgt gcccggtcag ggccccggtc acggtacgga 73920ccgccgggtg gtcttcgtag ccggtgggga cgagcagcag ccaggtgctg ctcacggcgg 73980gcggggcggg gtcgggcagc ggtgtccacc tgatctggtg gcgccatgac tccagggtgg 74040tgcgttcgcg gtggcggcgg cgccaggtgg acagtacggg cagggcgggc cgcagcgcct 74100cgatgtcctc ggccgtgccg tccggctcca gcacccgcgc cagcgcctcg gtgtcgaggt 74160cctcgatggc gccccacagt tcggcctcgg ccgggtcctg cgcgccgtcg ccgcggaccg 74220cacgggccgg gggcagccag tagcgctcgc ggtcgaacgc gtacgtgggc agggccgtcg 74280tccggggggc gggcgggccg tcgaaccagg gccgccagtc caccggcacc ccggaggtga 74340acgcctgcgc cagcgcggtg gccagctgtg cggggccgcc ctcgtcgcgg cgcagcgtgc 74400ccatggccac cgcggcgacc tgggcggact cgaagcactg ctggagcgcg gggacgacca 74460cggggtgggt gctgacctcg atgaacaccc ggtgccccgc ggtcagtagc gcgtcgaccg 74520cctcggtgag acggacgggc ctgcgcaggt tcgtcagcca gtagcctgcg tccagggcgg 74580tggcggtgtc catcgggccg ccggtcacgg tcgagtagaa cgccacatcc gtggcctgac 74640cgccgactcc ggcgagacgg gccgtcacct cgtcggcgat ctcgtccacc tggggaccgt 74700gcgaggcgta gtccacatcg atggtgcggg cccgcgcacc ggtcgcctcg caggcggcga 74760cgacagcggc cacggcgtcg ggcgggccgg agaccacggt ggaggacggg ctgttcagcg 74820ccgccacccc cacaccggcc gccttctcgc ccacctcggc gacgagccgc tcggccgtgt 74880cggggtcgac gcccagggag gccatcgcgc cacggccggc cagcgcccgc agcgcctgcg 74940agcgcagggc caccacccgg gccccgtcct ccagcgtgag tgcgcccgcg acacacgcgg 75000ccgcgatctc gccctgggag tggccgacga ccgccgcggg ccgcacgccg tggtgggccc 75060acagggcggc cagggagacc atcagcgccc acagcaccgg ctggaccaca tcgacgcggc 75120cgaggtcggc ggcgccgtcg gcgccccgca acacctccgt cagggaccag tccacgtacg 75180gggcgagcgc cgcctcgcac tccgcgatcc gggcggcgaa caccggtgag gcgtccagca 75240gttcggcgcc catcccgggc cactgggagc cctgccccgg gaacacgagg accacccccg 75300cgcccggcgc gcccggcgcg ccggtcacca cgtggggcgc cggctccccc gccgccagcg 75360cggccagccg ctcccgcagc gtcgcgccat cggggccggt gacgaccgcc cggtgctcga 75420acaccgagcg cgtggtcacc agcgaccagc ccaccgcccg cgggtcgccg tcggtcgccg 75480cgtcgagcag ccgccgggcc tggcggcgca gcgcggccgc gctgcgcgcc gacagcaccc 75540agggggtgac gagcgcggcg ggctcggcgg gctcggcgga ttcgttggca ggtggtgctg 75600gttcgggggc ctcttccagg atcaggtgtg cgttggttcc cgagaaaccg aaggcggaca 75660cgcccgcccg ccgggggcgc tcggcgcgcg gccactcgac ggcctggtcc agcagccgca 75720ctccgccggt gtcccaggcc acatgcgggg tgggctcgtc gatgtgcagg gagcgcggca 75780gcatcccgtg gcgcagcgcc atgaccatct tgatcacgcc ggcggcaccg gcggcgatct 75840gggtgtggcc gatgttggac ttgatcgacc ccagccacag cggccgcccc tcgggccggt 75900cctggccgta ggtggccagc agcgcctggg cctcgatggg atcgccgagg ctggtgcccg 75960tcccgtgtgc ctccaccgcg tccacgtccg ccggcgcgag ccgggcgttg gccagcgcct 76020gctggatgac gcgctgctgt gacggcccgt tcggcgcggt cagaccgttg ctggcaccgt 76080cctcgttgac ggcggagcca cggatgaccg ccagcacctc gtggccgttg cggcgtgcgt 76140ccgacagccg ctccaggagc accacaccgg cgccctcggc gaggttcatg ccgtcggcgt 76200ccgccgagaa gggcttggca cggccgtccg gggccagggc gcgcagctcg ctgaacccga 76260ccagcggtgc cggtgaggac atcacgtaca ccccaccggc cagcgccagc gtcacctctt 76320cctcccgcag cgcctggcac gccaggtgga tggcgaccag cgccgaggag caggcggtgt 76380ccagcgtcac cgcggcgccc tccaggccga gggtgtacga cacgcgtccg gcgaccacgc 76440tcggcgagtt gccggtggtc agctggcccg ccgcgccctc gggcacctgg aaggagttga 76500ggaagtagtc gaggccgtcg cagccgaggt aggtgccggt ggcgctggag cgcagggtgt 76560gcgggtcgat gcgggcgtgc tcgacggctt cccaggccag ctccagcgcc agccgctgct 76620gcggggccat ggtgaccgct tcgttcgggc tgatcccgaa gaacgcggcg tcgaaccggg 76680cggcgtcgcg gaggaagccg ccctcgcgca catagctggt gcccggatgc ccgggttcgg 76740ggtcgtagag cgcttcgagg tcccagcctc ggtcggcggg caggccgccg gtgccctcgc 76800ggccggcggc gaccaggtcc cacaggtcct cgggggaccc cgcatcgccg gggaaacggc 76860agccgatacc gatgatcgcg acgggttcgt ggcgggcgta ctcgacgtcc ttcagccggc 76920gctgggcctc gcgcagatcg gccgtcacgc gcttgaggta gtcgaggagc ttcttgtcgt 76980cgttcgccat gtcaggaccg ttccggcgcc ggcttgtcca cgaggcgcgc caggtcgagc 77040aggtcgctcg ccacccgctc gggttcttcc acgtggaggt tgtggtcggt gtcgacgtac 77100cagcgggatt ccgcctgcgg gatggccgcc tcggcctccg ccacccactt ccgggcgtag 77160gtgccccagg actgggcgtg ggcgggcagg gcgacgagca gcagaacggg cgccgtgatg 77220ccggggtacc agcgggcggg cgggtcctgc cacatgctgt ccgcgagcga catgtagtgc 77280tccagcggca gccgctgcac gagcagcccg tccggccctt ccaccatgtc ggcgaggctc 77340gcctcgacgg cggtctgtga ccacgtcggg tggagacccc gcaggagttc ccgcatggtc 77400tcctccttga ttccggtgac gtcgcgccac catccggcga gctccacgct cttctcccgg 77460gtgggggcgt aacgagaggt gtcgacgaag ccgatccagc cgccgtccac cagcgcgagg 77520ccggcggcca gctcggggtg ctgggccgcg agccgcaccg cgaggtttcc gccccaggag 77580tggccggcga acagcgcccc ggtcagccgc agctcgcggc agacggccac caggtccgcc 77640acggcggtcg cgttgtcgta cccgtgctcc ggcaggtcgg agtcaccgtg tccgcgcatg 77700tcgagcgcgt agacggggtg gccggcggcc gcgagcagat cggcgacctc gtcccacagc 77760cgggcgttgg accccagccc gtggagcagc aggaacggcc ggccgtcgcg gcccggccgg 77820tgccgcacgt tcaaatgcac tgcgtggtct accggaacgg acaacttcat ggcggggatc 77880ccttgcctcc tgggagaggg gttgagacgg gtgaccgatc gagcgggatc gcggcgcctc 77940ggcggaggct aggccgcatc cgcttcgtag gtctccgcga ggtacttgcc gagcagggtg 78000gaggtggggt gttcgacgat cgcgacgagg gggacttcga gcccggtgtc gctcatcagg 78060ctcttggcca gttgcacggc gctcagcgag ctcaggccgt tctccaggaa gttgctctcc 78120tcgtccaggg cggggacgtc gaggacctcg gaggcgcggc ggagcacctg cgcggccagc 78180agccgctggc gttcctccgg cgtcgcggac gcgagacgtg cgaccagctg ctcgtcgtga 78240gtggcttcct cgttgggttc catggctgca cccttctcgg tgtcgtatcg ggctgacggg 78300ggtacgtcgt ggtctggtgc ggtgggctag ccgagcgcgc ccttcgcggc gatgccggac 78360ggcttgagcc cgaccatctc cagctcctcg gggtggaacg gcggttcgac gagcttcggg 78420tggctggtgt cggtgcgcag caacgacagg aagcgggagt cgcccagggc cttctgcggc 78480gcgttcaggc tcagcacgct ggtggtgacc tcgcacacct tcggcgagcg gatcgacttg 78540ccggagagga agtcggagaa cttcagccgc tccgcgacgt ccgggccggt cagccgcggg 78600tcgctggaga ggttgcggca gttgacgtac tggatgtcgt tcagcccggc catgatccag 78660ggatcgtcca ccacctcgct cagcgcgcgc tggacctgcc gggtgccgcc ctcgccgaag 78720ccgtcgcgtg ccagctcctt gtccagggcc tcggcggcac gggccgccga gctcatgccg 78780tggccgtaga ccggattgaa cgccgccagc gagtcgccga ggatcagcag gccctccggc 78840cactgcggca tgcgctcggg gtacatgcgc cggttggctc ccgagtgcga ctggaagatg 78900gggctgatgg gctcggcgac gctcatgagt tcgctgacga tcgagtgccg caggaccttg 78960gcgtagccgg tgaactcgtc ctcgtcggtg ggcagcgggg cgccgcgcgt gctggtcagg 79020gtgaccagcc agcgaccgcc ctcctgcggg tagaccacgc cgaagcggcc cggttgccgg 79080gtgagggggt cggcggcgac ctggaccgcg gggaagtttc cgtccgcgcc ctcgggcgcc 79140ttgaacatgc gggtcgcgta gccgatgccg gcgtcgacca cgtcctgctc gacggccggc 79200aggccgaggg ccgccagcca gtggcccagg cgcgaggcgc gccccgtggc gtccaccacc 79260aggtcggcct ccagcagcct gggttcgccg ccgcccgcgt cccgcacccg caccccggtg 79320accctggagc ggtccccggc cagctcgacg gcctcggtgc cgtgctccac ctcgatccgg 79380ccgccggcga ggatcgcgtc acgcaccacc cagtcgagca gggggcgggt gcacatcatc 79440gcgtacgcgg tggccgggaa gcggtactgc cagccccacg ccgtcagggt caccaggtcg 79500ctctggaagc cgatccggcg ggcaccggcc gccagcagcc gttcggcggt gcccggcagc 79560aggttctcga cgatgcccgc gccactggac cacaggacgt ggacgtggcg ggcctggggc 79620tgtcccttac ggtggtgcgg tccgtcgggc aggatgtcac gctccaccac ggtcacggat 79680tccaggtggc gggccagcac atgggcggtg agcatgccgg cccatccgcc gcccagcaca 79740acggcacgtg tcggtgtggt catggcacca agtcctctcg gagcgggctc gtctgcgcgg 79800acactggcct ttcgcccagg acgcccatca cgagggtgtc ccgaacggct taagtgcgtc 79860tttccgttcc ggccgccgcg cggtgtccaa agccggctcc ggtgcggtgt cgtcgtcggg 79920gaacccgatc gtcatgtcgg tgaggcccca gaacccctgg atgtgtgcgc ccagtcggtc 79980ctcgttgacc tcgcagatag tgatcatgcg catgcgcatc ctggccggga cgtagacccg 80040cgcgtccccc aggaccacca cgaaccgtcc gtccatcgac gtgacgagcc gccccagcat 80100ctcgtgcacc ttgccctccg agccccggaa cgcgtgctcg cgcagggcgt ccttgccgcg 80160cagcaacagg ccgcccaccg ggtcctcgaa gacgatgtcg tccgtgaaca gggcgacggt 80220cccttccacg tccccctttt gcagcagccg gaggtagttg tggggcagct ccctgagggc 80280cgcctcgtcg gggtcgatcg ccgggggccc gacgaacggc cctgacctgc cgtcgacgga 80340ggtctcgagg tcgggtttgc cccagtacgc ccgcatgtcc tcgatgaggc cggactcgcc 80400gacgcggagc agcagcgcgt agtggcagcg cagggcggtc ggctccgcgc cctcgggcgc 80460gggcagccag ccgcggtcgg cgtacagcgg gccgcggggc cggtagtcca tgaccgcggt 80520gatccgggac aggacgtgtt cgccgtcctg ccccacgacc gactccacga tcgtctcgtc 80580gacgtttccg gcgactgcgc tctcgaagtg cgcgcgcagc gcctcgtggc cggcctggcc 80640gccggccccg acgggggcct cgaacgtgac gtccttcgcg tacaggtcga gaagcccgtc 80700gatgtcgccc tcgctgaggc gccggctgtg ttcgcgtgcg atcttcttgc gggtgcgctc 80760gttgagcacc gtatctcctc tgcttaccgg tcgggccgta ccaggctcat gtcgaacgcg 80820gcgcgccagt accgtccgcg ccggaaggcg taggtgggca gctcgacgcg gcgggcctcg 80880ggcccgaaga ccgcccgcca gtccacggcg gtgccgcggg catgcgcccg ggcgaggccg 80940gtggcggcgg aggtggcctc cgcgacgccg ggccgcagcg cggccaccgc gtgcgccccc 81000gcgccgtcga ggagtccggc cacctcgtgg ccgcccagtt ccaggtagac cgtgccgtcc 81060ccggccaggt cacggacccg ctgggcgaag tcccgcggcg ggtccggggc ctcgtgcggg 81120ccggtcagcg ccgcgcacgc gtcgggcagt gccagtcgcc cgctgacgtg gtcttcggcg 81180aggcggccga tgccgtggcc gcgcaccgca ccgggcctcg tcccccacga ctcgaccagt 81240cggaacagcg ccacctggat cgcgaaggcc ctggccgggt cggggacggc cgccgaggtg 81300gggtccgcgc cggtgagcat cgcgtcacgg gcggggaagc cgaggtgggc gtcgagcgcc 81360gcgcagaccg tgtcgaacgc gtcggcgaac gccgggaagg cgtcatacag ctgcttgccg 81420gtgccgggtg cgggggcgtg gcccgtgaac aggacgacga gccggggccg gcgtccgatc 81480gcgccccggg cgacatgggg cgcagccctt ccctcggcca gtgcgcgcag tccgtccagc 81540agctcctggc ggctctcgcc gacgaccacg ccgcggtgtt ccagcgcggc ccgggacgtc 81600cccagcgaca ggccgacgtc ggccggccgg agcccgggcc gctcgtcgag gtgggccagc 81660agacgggccg cctggtcgcg cagcgcgtcg gggtcgcagc cggagagcgg ccagagcgcc 81720accccggcgc ttcccggctc tccttccgct tcgggtagcg ggggcggagg gtcgtcttcg 81780ggcggcgcct gttcaacaat cacatgcgca ttggtgccgc cgaatccgaa cgcggacacc 81840ccggaccgaa gtggccggtc agttcccggc cagggggtgg cagcggtgag cagccggatc 81900cgaccccgct tccaggagat gtgcggcgac ggcacctcgg tgtgcagggt gcgcggcagg 81960acgccgtggc gcatggcctg gaccgtcttg atcaccccgg ccacaccgct ggccccctgc 82020gtgtggccga ggttggactt caccgccccc agcagcagcg ggcggtcggc cgggcggccc 82080tcgccgtaga cggacgccag cgcctgggcc tccaccgcgt cactgagcag gccaccggtg 82140ccctggccct cgaccgcgtc gatctcgtgc ggcaggagcc ccgcggcggc cagcgcctgc 82200ctgatcagcc gctgctgggc ggggccgttc gacgcgctca cgccgttgtt ggtgccttcc 82260tggccgatcg cgctgccacg gaccacggcg agcacgggac gcccgtgcgc gcgggcggtg 82320gagagacgct ccaggagcac cacgcccgcc ccctcggcga aggcgatccc ctcggccccg 82380tcggcgaacg agcggcagcg ggcagcggcg ggcagcccta tcccccggcc ggtcccgatg 82440aacgtctccg ggctggacat cacggtgacg ccgccggcca gagccatgga acactcgccg 82500ccgcgcagcg actggcaggc caggtgcagc gccaccaggg atccggagca ggcggtgtcg 82560agggtggccg tgggcccttc gaggccgaag acggaggcga ggcggccgga cagcacggcg 82620ctcgaaccgc cggaggcgat gtggccgagc agctcgtcgg ggacgtcctt caggtgcggc 82680gtgtaatcct ggccggtggt gcccatgaag acgccacagc gccggccgcg gaccgaccgc 82740gggtcgatcc cggcccgctc gaacgcctcc caggtggtct ccagcagcag ccgctgctga 82800gggtccatca ccgaggcctc gcccggggag atcccgaacg gcgcggggtc gaacgcgccg 82860gcgtcgctga ggaaacctcc ctcctcgacc gcgctgcgca cgtccgggtg ggcgtccggc 82920ccgtacaggc cgtccaggtc ccagccccgg tcggcgggaa aggcgccgat ggcgtcccgc 82980tcctcggtca gcagtgtcca caggtcgtcc ggcgactcga cacccccggg atagcggcag 83040gccatcccgg tgatcgcgac gggctcccgg ggtgccgcgc gcagccggtc gttctccttg 83100cgcagccgct ctgtctccag gagcgaggtg cgcagggcct cgacgacctt ggcgtcggcc 83160actgtcatga cattcctccg aagggacggc acacgtcagg actcgtcgtt gcgcagggcc 83220gcgttcacca gttcctcgag gtccatggag gcgatgtccg cggagtcggg ggtgtgggtt 83280tcatcgcggg ttccttccac gggccgcgcc cccgtggtgt ccgcttcccc gggcccctcg 83340cccagccgca gcagtgtctc cagcaggccg gactcctcca gccggcgcag cgggatggcg 83400gccagcacct gccggacccg gctctcgcgc ggatcggtgg acgggccctc cggcaggccg 83460gggggcgggc tcgggtcggc gggtgtactc gggtcggtgg gcggtcccag ctcggtgggc 83520ggggcgatct gctcgcgcag gtgctcggcg agcgccacgg ggttggcgaa gtcgaagacg 83580aacgtggcgg gcaggcgcag gccggtggcg gccgagagcc ggttgcgcag ctcgacggcg 83640gtcagcgagt cgaagcccag ctccttgaac gtccggtcgg gggcgatctc gtcggccgag 83700tcgtggccga ggacggccgc catgtgcgcg cgcaccgcct ccagcacaca gctgtcgcgt 83760tccgcctccg gcagcgcggt gacccggtcc gtcagcgacg ggccgccggt gcccgccacg 83820ccgctgtccg cgacccgccg cgccctggtg cggccggcgg ccgggccgcc gcgcgaggcc 83880gcggtggact gccggatcgg gaccagcagc gcgtcaccca cggcgtacga ccggtcgaac 83940agcgccaggg cctgctcggt gggcagcgga tccataccgg cctggctgat gcgctgccgg 84000acaccgtcgt ccagctcacc agtgagcgcg ctggtctcct cccacattcc ccaggccagg 84060gactgcgcgg gcagcccgcc ggcctggcgg tgctgggcca gggcgtccag gaacgtgctg 84120gcggccgcgt agttggcctg cgaggggttg ccgtacaccc cgacgatgga ggagaacaac 84180acgaacagcg ccaggtcggc gtcctgggtg agctcgtgca gatgcagcgc ggcctcggcc 84240ttcggccgca ggacccgggc cagttgttcg ggggtcagcg cgtcgaccat gccgtcgtcg 84300aggacgccgg cgcagtgcac caccccggtc agcgggtggg cgtcggggac gccggccagc 84360acctcggcga gccgctcgcg gtcgccggcg tcgcaggccg tgacggtcac ggtggcgccc 84420agcgcggcga ggtcctcgcg cagggcgggg gcgccgggtg cctcggggcc ccggcggctg 84480gtcagcagca ggtgccgcac cccgtgtcgg gtgaccaggt ggcgggcgat cagaccgccc 84540agggtgccgg tgccgccggt gaccaggacc gtgccgtccg ggtcgagcgg cctcggcagt 84600gtcagcacga tcttcccgat atgagcggcc cggctcatca gccggaacgc gtcgcgcgcc 84660cggcggatgt cgaaagcgcg gaccgcgggg tgggtcagct cgccgcccgc gaaccggccg 84720aggatctcgg tcagcatgcg tccgatgccg tcctctcccg gctccctcag gtcgaggacg 84780cggtagcgga ggccggggta cggctcggcg agctgctcgg ggtcgcggac atcggtcatg 84840cccagctcca cgaaccggcc cttctcggcc agcaggcgca gcgaggcgtc ggtgtggtcg 84900ccggtgaggc tgttgaggac cacgtcgacg ccgcggccgc cggtggcggc gcggaaggcg 84960tcctcgaagt ccaggtcgcg ggaggaggcg cggtgggcct cgtcgatgcc catcgcctcc 85020agcacatggt gcttaccggg gccggcggtg gcgtagacct cggcgcccag gtggcgggcg 85080atctgcaccg ccgccgtgcc gacgccgcca gtggccgcgt ggatgaggac cgactcgccc 85140gccttgagcc cggcgaggtc caccagcccg taccaggcgg tcacgtacgc gatgggcacg 85200gccgccgcct gctcgtcggt ccagcccggc gggatcggca cgacggagcg ggcgtcggcc 85260acggcggtcg agccgaaggc cccctggaac atgcccatca cgcggtcgcc gacggccacc 85320gaggtgacgt cctcggccac ctcgagcacc acgcccgcgc cttcgctgcc gcggaacgcc 85380ccgtcgcccg ggtacatgcc gagcgcgatc agcacatccc ggaagttgac gcccgcggcg 85440cggacctgga cgcggacatg gccgggggcg ggcggcccgg gttcctccgc cgccaccagc 85500cccaggttgt ccagcgtggc gggagcggcg atgtccagcc gccagtgggg ggtttccggg 85560agggccaggg cgccgccgga caccgcgggg accaggcggg acacgcgcac cgtcccgtcc 85620cggacgacga tctgcttctc accggcggcc accgcgacgg cgacggccgc ggcgaccgcc 85680ttcggcagtt cgggcccgtc gaggtccacc agggccagcc ggtccgggtg ctcggactgc 85740gcggagcgga tcaggcccca caccggggcg tgcacgaggt cggtgacgtc gtcgccctcg 85800aaggcggcga ccgcgccgcg ggtgaccacc accagccggg aggcggcgaa ccgctcgtcg 85860gcgagccagc gctggacggt gtgcagcacc tcgcccagcc ggcggcgcag cgcaccgggg 85920aggtccccgt cgtccggcgc cggttcgcag ggcagcaggg tcaccggcgg ggcgggcagg 85980ccgccgtcca ccgcggcggc gagcgcgtcg aggtcgccgt agcggcgcac tccgtagtag 86040gtgggctcgc tgccggtggc gaccgcggtc cagtcgtcgg tggcggtgcc cggctcgggg 86100tcggtgagcg gggtgtacgc cacgcggaag agcccgtcgc gcgtctcgtc gccgtcgctc 86160gggagttgcc ccgccgtgaa cggccgggtc accagggact cgatcgagat caccgggtgt 86220cccgaggcgt cggtggcgtg gacggacatg ccgtcgtcgc cgaccggcgc gagtctgacg 86280cgcagcaact cggcgccggt ggcggccagc gacacgccgt tccaggcgaa cggcagcgcc 86340atccggcctc cgctgtcgcc gcgcaccgcg ctcagtccgc tggcgtgcag cgcaccgtcc 86400agcagggcgg ggtggatgcc gaacgcggcg gccttcccgc gctgctcctc cgggaggacg 86460acctcggcga agacctccgt ctcgcgccgg tagacggcgc gcagcgagcg gaaggcgggg 86520ccgtagcgga aaccgccctc ggcgagctgg tcgtagcagc cttccacggg cacgggccgg 86580gcgccttcgg gcggccactg ggcgaggtcg aacggccggg cggccccgcc gggcgccaag 86640gtgccggtga cgtgctggac ccacggccgg tcggacccgg acgggcgggc gtacccgccg 86700agcgtacggc ggccgtcctc ggcggcgccg cccaccacga gctggaggtc gaaaccgccc 86760tgctcgggga ggaccagcgg gctcgccagc accatttcct cgaggtggtc gcagcccacc 86820tcgtcaccgg ccctgacggc cagttccacg aacacggcgc cgggcaccag caccgcgccg 86880gccaccgcgt ggtcggccag ccatggatgg gtgtccaggc cgagccggcc ggtcatgacc 86940acctcgtcgc cttcggcgag gagggtgacg gctcccagca gcgggtggtc ggccggcgtg 87000aggccgagac ttgcggggtc gcccgccgcg ccggcggtgg catccagcca gtaacgccgc 87060cgctggaacg cgtaggtggg caggtcggcg gggctggtgc cgtccgggaa cacccggctc 87120cagtccaccg cgacgccatg ggtgtgcgcc tggccgagcg cgaggaggaa gcggtcccag 87180ccgccctcgt cccggcgcag ggtgccgagg gccgtggcgt tggcgcccga ctggtccatg 87240gtttcctgta cgccgatggt gaggaccggg tgcgggctgc actcgatgaa cacgccgtgc 87300ccgtcggcca ggagcgcctc ggtggcctct tggaacctca cctgttggcg gagattggtg 87360taccagtagc cggcgtccat ggtggtgccg tcgatacgcc gcccggtgac ggtcgagtac 87420agcgggacgg tgcctgcctt cggctcgata cccgagaggg catcgagcag cgggccgcgc 87480agcgcgtcga cgtgggcgca gtgcgaggcg tagtccaccg ggacgcgccg ggcgcgcacg 87540ccatcggcct catagccctc cagcagctcc tcgagggcgt cggcgtcgcc ggagaccacg 87600gtcgaggagg ggccgttgac ggcggccacg ctcagccgcc cgtcccagcg ctccaggtgt 87660ttctccacca gcccgacggg ctgcgccacg gaggccatac cgccacggcc ggacagctcg 87720ggaagcacct gggagcgcag caccacgacg cgggccgcgt cgtccaccga cagcacgccc 87780gcgacacagg ccgccgcgat ctcgccctgg gagtggccca ccacggccgc gggctccaca 87840ccgtgggaac gccacagctc ggccagggcc accatcaccg cccacagcac gggctggacc 87900acgtccaccc ggtccagcga gggggcgccg ggatcggccc gcagcaccga ctccacggtg 87960tagtcggtgt gccgttcgac cgcttcggcg cagcggcgga agtgttcggc gaacaccggt 88020gaggcgtcgg ccagcgcgag ggccatgccg acccactgcg agccctggcc ggggaagacg 88080aacgccgtcg cgccggggct caccgaaccc gtgacgacgt ggccggcggg ggtcccctcg 88140gccagcgagc ccagcccggc caggagggtg tcgcggtcgg cggcgaccac ggcggcacgg 88200tgcgactgcg cggcgcgcga gcccgccagg gcgaacccga cacgggtggg gctcgcctcc 88260ggccgctcgg cgaggaagtc ccgcagccgg gcggcctggg cgcgcagcgc cgcctcgcca 88320cgcccggaca ccagccacgg gaccaccggc gactcggcgg cgggggcggg ctcctcggcc 88380ggcggggcct gttcgaggat gacgtgcgtg ttggtgccgc tcatgccgaa ggaggacaca 88440ccggcccggc gcgggccgcc ggcctcgggc cacggccgcg cctcggtgag cagccgtacg 88500gtgccctcgg accagtccac ctgatgggtg ggttcgtcca cgtgcagggt gcgcggcagg 88560agtccgtggc gcagggcgtg caccatcttg atcaccccgg ccatgccggc ggcggcctgg 88620gtgtgcccga tgttggactt gaccgagccc agccacagcg ggccgccgtc ggcgcgccgc 88680ctgccgtagg cggcgatcag cgcctgcgcc tcgatggggt cgcccagcga ggtgccggtg 88740ccgtgcgcct ccaccacgtc gatgtcccgg gcggacagct gggcgtcggc cagcgccgcg 88800cggatgacac gttcctgggc ggggccgtgg ggcgcggtga ggccgttgga ggcgccgtcc 88860tggttgatcg cggagccgcg gatcacggcg agcacggggt gcccgaggcg ttcggcgtcc 88920gccagccgtt ccaccagcag gacgcccacg ccctcggcgg gcccgaagcc gtcggccgcc 88980gccgcgaacg ccttgcaccg gccgtcggcg gcgagcccgt tcagcttgct gaactccaga 89040taggtgccgg gggtcgccat cacgcacact ccgccggcca gcgccaggga gcactccccc 89100ttgcgcagcg cctgcaccgc caggtgcagg gccaccccgg acgaggagca ggcggtgtcc 89160accgtgaagg aggggccctc gaagccgaag gtgtaggaga tgcggcccga catcgagctc 89220gcggccagcc cggtggccag gtacccggcg acgtcctgcg gcacgcccgc ggcgttgccc 89280gcgtagtaga cggtgctgcc gcctacgaag acaccggtct tgctgccgcg cagtgaggtc 89340aggtcgatgc gggcgcgctc gatggcctcc caggaggtct ccagcagcag gcgctgctgc 89400gggtccatgg ccagcgcctc gcgcgggttg atgtcgaaga agtcggcgtc gaagtcggcg 89460atgtcgtgga cgaatccgcc ctcgcgcaca taactggtgc cggtctcgcc cgagtcgttg 89520aacatgccgg ggtcccagcc ccggtcgtcg ggcatggggg agatcacgtc gcggccctcg 89580gacaccaggt cccacagggc ctccggagtg tccgtcccac cggggaaccg gcaggccatg 89640ccgacgatcg cgatcggctg gcggtcccgg gcctccacct cgcggaggcg ctgctgagtg 89700tcatgcaggt cggtcgccac ccgtttgagg tagtcgacca gctgttcctg gttcgccatg 89760gcgtcctctt tcaggccctt cccaggtggg agtcgatgaa ctgcagtact tcgtcggccg 89820aagcctcctg gagccgctcc gcgacgccat tgccctccgc agccgtgggt ttctggcggc 89880accgcgtcag cagcgcttcg aggcgggctg tcacggcgtc ccgttccccg tcgtcgacga 89940gcaggtcgac gtggtcctcc agccggtcga gttcctggat cagggcctcc gacccgctca 90000gcccctcgga gaccagttgt tcccgcaagt gctcggcgag cagtgcgggg gtggggatgt 90060cgaagacgag ggtggagggc agcgtggtgt cggtggcccg gctgagccgg ttgcgcagct 90120cgaccgcggt cagcgagtcg aagccgatct ccttgaaggg ccgcgacgga tcgaccgcga 90180ggacatcgcc gagcccgagc acggccgcca cgttgcgccg caccagctcc agcagggtgc 90240ggctctgctc ggccgccgag aggcccgtca ggcgctccgc catcgtcagg tcgtccccgg 90300tggcgggctg ccccgccgcg gcggcgcgcc gggcaggggc ctgcctcacc aggccgctga 90360ggatgcgggg caggacacgg gcgtcggcga ggtcccgcag cgcttccggg tcgaggcggg 90420cgggtaccag caggctttcg ggacggcgct cggccgcgtc gaacagggcc aggccctgct 90480cggacgacat cgcccggata ccggagcgct ccatacgggc gatgtcgcgc tcgtccaggg 90540tgccggtgat gccgctggtc tgttcccagt acccccagga gagcgactgg ccggggaacc 90600cggcggcgcg gcggtcctgg gcgagcgcgt ccaggaaggc gttggacgcc gtgtagttgc 90660tctggccgcc gacgccggtc actccgccgg tcgaggagaa cagcacgaag gttgcgaggt 90720ccttctcgcg ggtcagctgg tgcaggttga ccgccgcgtc caccttgggc cgcagcaccg 90780cgcgcagccg ctcgccggtg agggagggga tggtgccgtc gtcgatcacg ccggcggcgt 90840ggatgacgcc ggtcagcggg tgcgccgccg ggatgtccgc cagcagctcc gccagggcgt 90900cgcggtcggc ggcgtcgcag gcggcgatcg tcacctctgc gcccagggag gtcagttcgt 90960cgcgcagctc ggcggcgccg ggcgcgtcgg cgccccgacg gctcgtcagc agcaggtgac 91020gggcgccccg cgtggtgacc aggtggcggg cgaccacgcc gccgatcgtg ccggtgccgc 91080cggtgatcag tacggtgcct tcgctgtcca gacgccggcc gggcaggtcc ggcggggtgg 91140gggcggtgtc ctggccgata cgggccaggc gcgggacaac cacacggccc tggcgcaccg 91200ccacctgcgg ctcacccgcc ccgagcgcga cggacagggc gccgggcagc agcgccgccg 91260cgtccgggtc ggcgccgtcc aggtcgacca ggacgaaccg gtcgatgttc tccagttgcg 91320cggagcgcac cagcccccac agggcggagt gcaccaggtc ggtgacccct tcgtcgccgc 91380tcgtcgactg ggcgccgcgg gtgaccagca ccagccggga gtcggcgaac cgcccgtccg 91440cgagccagcg ttgcacaacc ccgagcagcc gtgcggtctc gtcgtggacc gcggcggcga 91500gtccggggtc gagggcggtc gctccgccgg cgaggggtgc gcagtccagc accatcaggt 91560cggaaacggg ggcgtcgtcg tcgacgtacc gcgccgactc cgcgagatcc gtggtggtgc 91620gcacggcatg cccggcgcgt tccagcgcgt tcgtgatgcc gaggccgtcg gcgcccacca 91680cttcccacgc gcggtcggcg agcacctccg gcctgggctc ggcggagatc cactcgacgc 91740ggtagagcga gtcgagctcg gaaccgcccc ggctcagctg ctccagggag accgggcgga 91800agacgaccgc gtcggcggag gcgacctgac ggcccgaggg gtccgcgaca ctcagcgaca 91860ccccgtcggc cgtttcgttg gggaccatac ggacgcgcag cgccgaggcg ccggtggcgt 91920acagcgacac gccgttccag gtgaacggga gcagcatctg cccgtcgggc gggccgccct 91980gcccgatgcc gccggcttgc agcgcggtgt ccagcagggc gggatgcagg ccgaaccgtc 92040cggccccgcc gtgcagttcc tcggcgagcg cggcctcggc gaacacctcg tccccccggc 92100gccaggcggc cttcaggccg cggaacgccg gcccgtagcc gtagcccgcc tccgcgagac 92160cggcgtagag accgtccagg gacagcggct cggcgccggc cggcggccac tgggtgaagt 92220cgaagtcgtc cggccgggca cctgtggtga gggtgccgct cgcgtgccgg gtccacggcc 92280ggtcggcgtc gtcggggccg tcggcgcgcg cgtggacggt gaagccgcgg ccgccgtcct 92340ggcgtggcgc ggagacgacg agctggagct ggagggcccc gtcctcgggg aggaccaagg 92400gggtttccag gacgagttcc tcgacgcggt cgcagcccac ctcgtccccc gcccgcacgg 92460cgaggtcgac gaacgccgtg ccgggcagga acaccgaacc ggccaccgcg tggtcggcca 92520gccaggagtg tccggcggtg gagatccggc ccgtcagcaa cacctcctcg ctctcggcga 92580cccggacggc ggcgccgagc agtgggtggt cggcggaggc gaggccgaga ctgtggggat 92640cgccctggcc gccgatggtg gaggcgtcga tccagtagtg ctggtgttgg aacgcgtagg 92700tggggaggtc caggacgggc ggggccgggg tggtgacgcc ggtggggaca tcaggagtgg 92760tgtccacccc gtaccaggct ttccagttca cggacagacc cgcggtgaag gcctgcgcca 92820aggcccgggc cagctgcacc ggaccaccct ggtcccgctg cagggtcgcc acggtggcca 92880cagggacatc caacgcctgg gcggtctcct gaatgcccac gctcagcacc ggatgcggac 92940tcgactccac cagcacccga tatccatcgc tcagcaacgc ctcaacggcc gtggcgaacc 93000gcacctgccg acgcaggttc tccacccagt acgccgcgtc cagcgccgtg gactccaccc 93060ggccaccggt caccgccgaa tagaacgcca cagccgtctc aaccggcgtg acctgcccca 93120acacctcgat caactcatcg gcgatgtcat ccacctgcgg attgtgagac gcataatcca 93180catccaccag ccgggcccgg ccgccggccg cctcaaccgc cgcgaccacc tccgccaccg 93240ccaccggcgg cccggaaacc accgtcgaac ccggcccgtt caccgcggcc accaccaccc 93300ccgcacggcc gccgatcgcc ttctcagcct cctcccgacc caccgccaac gacgccatcg 93360ccccacgccc cgacaacacc cgcaacgccc gagaacgcaa agccaccacc cgcgcaccat 93420cccgcaacgt aagcgccccg gccaccaccg cagcagcgat ctccccctgc gaatgcccca 93480ccaccgccgc gggctcaaca ccatacgaac gccacacctc cgccaacgac accatcaccg 93540cccacaacgc aggctgcacc acatccaccc gcgacacatc caccccatcc tcgccccgca 93600acaccgccgt caacgaccac tccacaaacg gcgccaacgc cacctcacac tccgcgatcc 93660gccccgcaaa caccggcgaa gcatccaaca actccacccc catcccccgc cactgaccac 93720cctgccccgg aaacaccaac accgggccgg ccccgccgac caccggttcc ccggccacca 93780cgccgcccga gggcagtcca tcggccaggg ccgtcagacc ggtcaacagc tccgtccggt 93840cctggccgac caccaccgcg cggtggtcgt gcaccgcccg accggccacc agcgaccagc 93900cgacctccgc cacctcgtcc acggtctcca ggacgaactc cgccagccgt ccggcctggg 93960cccgcagccc cgcggccgac cgccccgaca ccacccacgg caccaccccg cccaccggcg 94020ccgggccaca gtccacggga gcgtcgacgt acggatcctc cggagcctcc tccagaatca 94080catgcgcgtt cgtcccggag atgccgaacg ccgagacgcc cgcccgccac ggacggtcca 94140cctccggcca gtcggtcgcc tcgctgagca gtgcgaccgc cccgctgtcc cagtcgacat 94200gcggcgtcgg cgcgtcgatg aacaggctgg ccggcagctc cccgtggcgc atggccatca 94260ccatcttgat gacaccggcg gcgcccgcgg cggcctgggt gtggcctatg ttggacttga 94320ccgaccccag ccacaacggc cgctccgccg accgtccctg accgtaggtc gccagcagcg 94380cctgcgcctc gatcggatca cccagcgtcg tacccgtccc gtgcgcctcc accacatcca 94440catccgccgg cgacagccgc gcgttcgcca gcgcctgccg gatcacccgc tgctgcgacg 94500gaccgttcgg cgccgtcagc ccattgctcg caccgtcctg attgatcgcc gaaccacgca 94560ccaccgccca caccttgtga ccattacgcc gcgcatccga caaccgctcc aacaacagca 94620aacccacacc ctcgccccac cccgtgccgt ccgccgccgc cgcgaacgcc ttgcagcggc 94680cgtccgccgc gagcccccgc tgccgcgaga actccacgaa ggcgcccggt gtggacatca 94740ccgtcacccc acccgccaac gccagcgagc actcaccgcc gcgcagcccc cgcaccgccg 94800agtgaagcgc caccagcgac gacgaacacc ccgtgtccac cgtcaccgcg ggaccctcca 94860gccccagcac atacgacacc cgccccgaca ccacgcagcc gagattgcct gtgccggtgt 94920acccctccat gtcgaggctg gagatcccga tgatcgacag gtagtcgaag atggtcgcgc 94980cgacgtagac gccggtgtcg ctgccgcgca gggagccgtg gtcgatgccg gcgcgctcga 95040acgtctccca ggcggtctcc agcaacagcc gctgctgggg atccatcccc accgcctcac 95100gcggcgagat accgaagaag tccgcgtcga actcccccgc gtcgtagatg aatccgccct 95160cgcgcacata gctggtgccc gggcggtccg ggtccgggtc gtacagcgtg tccaggcgcc 95220agccgcggtc ggtcggaaac gccgacatcg cgtcccgccc ctctgtcacg aggtcccaga 95280gctgttccgg gctggtcacc ccgcccggga accggcacgc catcccgacg atcgcgatcg 95340gctcgtccgg ttccccgatc ggtcccgcga cggggacgac ggtggcgtcg gcgacagccg 95400tctgcgtgcc ggagatctct ccgaggacat ggcgggccag gtcgtccgcc gtggggtagt 95460cgaagatcag cgtggtgggc agcggcattc cggcgcttga gctgagcttg ttgcgcagct 95520cgacggcggt cagcgagtcg aagcccatct cgaagaaggg cttcgttgcg gaaacggcgt 95580ccggacccga gtggcccagc acgccggccg ccagggcgcg gatgtgctgc gagagtatct 95640ggtgccgctg ttccggggcg gcggcggaca gttgcctgcg caacgggctg tcctcggacg 95700gcgtctcctt ctcgaggagt tcgacggcct gggggatctc ggcgagcagc cggctggcgc 95760gctgcccggc gaacgtcgcg gcgaaccggg cccagtcgaa gtcggccacc gtcagcgtcg 95820tctcgccacg gcccagggcg tcgcccagca cccgcagcgc cagctccggc ggcatggcgg 95880tcaggccccg gtcacggaag aaggtggcca ccgcctgatc ggcggccatg cccgcctcgc 95940cccacggccc ccacgccacc gaaagccccg gcaggccctc ggcgcggcgg cgttcggcca 96000gcgcgtccag atacgcgttg gccgccgcgt acgcgccctg ctggccgctg ccccagctgg 96060ccgcgccgga ggagaacagc acgaacgccg acacgtccag gtcgcgcgtg agctcatcga 96120gccagcgcgc gccccccgcc ttcgccgccg tcacctgcgc cacccggtac ggatccaggt 96180cgcgcaccgg cgtgtagtcg ccgacaccgg cggcgtggaa gatcccacgc aggggatgct 96240cttccggcac ccgtgccagc agcccggccg cctggtcccg gtccgcgaca tcgcaggcca 96300ccacgtccac cgaggcgccg agcgcctcca gctccgccgt cagctcctcg gccccgggcg 96360cggcagggcc gcgtcggccg gccacgatca catggcgcgc gccccgctcg accacccacc 96420gtgcgacccg gccgcccaca ccgcccgtcc cgccggtgac caggaccgac ccctcggtcc 96480gccactcctc gcccggcgca cctcccggtg ccgcgcgcag ccggcgcccc agcgcaccgt 96540cggcgcgcaa agccacctgg tcctccgtgc cctcggccac caccgcggcc agccggctcg 96600ccgtctcctc ctccgccgcg gccggaagat cgaccagtcc gccccaccgc ttcgggtact 96660ccaacgccat cacgcgcccc agaccccaca cctgggcctg cgccgggctc accgggccgt 96720cctcgccgac cgacaccccg ccgcgcgtca cacaccacag gcgcccgccg aagtccatct 96780cgtccagtgc ctgaaccagg gcgatggtcc cggccagccc caccgacagg tgcgggtggt 96840ccgggtgtac ggcttcgtca aggcccaacg cgctgatcac accggcgacc tcgggcccgc 96900cctccagctg ccgcagacgc gtcgtgagcg gatcgcgcgc ctggtccccc ggcgccagcg 96960cgagggtcgt gcaccgcgcc ccccggccct ccagcgcccg gacgaccgtc tcgaccgccg 97020cgtcaccctc gagcttctcg ggcaccacca gcaaccatgt accgctcagc cggggggccg 97080cgtccaagcc ggtgaccgtg gaccaggcgg tccggtagcg ccacccgtcg atccgggctc 97140gatcccgtgt gcgctgacgc cactccgcca acaccggcaa cgccggggcc agcgcctgcc 97200gcgcggtgtc ctccgccacg ttcagcgagg ccccgagccc ggcgagatcc cccgcttcga 97260cagcgctcca gaagcgctcg tcgtcctccg agccctgctc cggagaactc ccccacctgc 97320cgggcttcag ccagtagtgc tggtgttgga acgcgtaggt ggggaggtcc aggacgggcg 97380gggccggggt gatggcgccg gtggggacat caggagtggt gtccaccccg taccaggctt 97440tccagtccac ggacagaccc gcggtgaagg cctgcgccaa ggcccgggcc aactgcaccg 97500caccaccctg atcccgctgc agggtcgcca cggtggccac agggacatcc aacgcctggg 97560cggtctcctg gacacccaca ctcagcaccg gatgcggact cgactccacc agcacccgat 97620atccatcgct cagcaacgcc tcaacagccg tcgcgaaccg cacctgccga cgcagattct 97680ccacccagta cgccgcatcc agcgccgtgg actccacccg gccaccggtc accgccgaat 97740agaacgccac agccgtctca accggcgtga cctgccccaa cacctcgatc aactcatcgg 97800cgatgtcatc cacctgcgga ttgtgagacg cataatccac atccaccagc cgggcccggc 97860cgccggccgc ctcaaccgcc gcgaccacct ccgccaccgc caccggcggc ccggaaacca 97920ccgtcgaacc cggcccgttc accgcggcca ccaccacccc cgcacggccg ccgatcgcct 97980tctcagcctc ctcccgaccc accgccaacg acgccatcgc cccacgcccc gacaacaccc 98040gcaacgcccg agaacgcaaa gccaccaccc gcgcaccatc ccgcaacgta agcgccccgg 98100ccaccaccgc agcagcgatc tccccctgcg aatgccccac caccgccgcg ggctcaacac 98160catacgaacg ccacacctcc gccaacgaca ccatcaccgc ccacaacgca ggctgcacca 98220catccacccg cgacacatcc accccatcct cgccccgcaa caccgccgtc aacgaccact 98280ccacaaacgg cgccaacgcc acctcacact ccgcgatccg ccccgcaaac accggcgaag 98340catccaacaa ctccaccccc atcccccgcc actgaccacc ctgccccgga aacaccaaca 98400ccggcccagc acccccagcc accggatcca ccgacaccac accaccgaac ggcacaccct 98460ccgccagggc ccgcaacccc gccaggagtt cattccggtc gtcgccgacg accacggccc 98520ggtgttcgag catcgcccgg ccggccacca gcgaccaccc cacctccgcc acatcggcgg 98580tggcctgctc ggcgaactcc gccagccgtc cggcctgcgc ccgcagcccc gcggccgacc 98640ggccggagac cacccagggc accacgccgc ccggggtggt cggctccgcg ttttccccct 98700ctgtcgacag tgcgggcgcc tcttcgagaa tcacgtgtgc gttggtgccg gagatgccga 98760acgccgagac gcccgcccgc cgcggacggt ccacctccgg ccagtcggtc gcctcgctga 98820gcagtgcgac cgccccgctg tcccagtcga catgcggcgt cggcgcgtcc acgtgcagcg 98880tctgggggag caggccatgg cgcatggcca tcaccatctt gatgacaccc gccacaccgg 98940cggcggcctg ggtgtgggcg aggttggact tgatcgaccc cagccacaac ggccgctccg 99000ccgaccgccc ctgaccgtag gtcgccagca gcgcctgcgc ctcgatcgga tcacccagcg 99060tcgtacccgt cccgtgcgcc tccaccacat ccacatccgc cggcgacagc cgcgcgttcg 99120ccagcgcctg ccggatcacc cgctgctgcg acggaccgtt cggcgccgtc agcccattgc 99180tcgcaccgtc ctgattgatc gccgaaccac gcaccaccgc ccacaccttg tgaccattac 99240gccgcgcatc cgacaaccgc tccaacaaca gcaaacccac accctcgccc caccccgtgc 99300cgtccgccgc ccccgcgaac gccttgcagc ggccgtccgc cgcgagcccc cgctgccgcg 99360agaactccac gaaggcgccc ggcgtggcca tcaccgtcac cccacccgcc aacgccagcg 99420agcactcacc gccgcgcaac gcctgcaccg ccgagtgaag cgccaccagc gacgacgaac 99480accccgtgtc caccgtcacc gcgggaccct ccagccccaa cacatacgac acccgccccg 99540acagcacaca cgcgaggttc ccggtagcgg cgtagccctc cacgtcactg ccggccatgg 99600cggtgagcat catgtattcc tgcgatgtca caccggcgta gacgcccgtg ctgctcccca 99660cgagaccgtg cgggtcgatt ccggcgtgtt cgaacgtctc ccaggcgacc tccagcaaca 99720gccgctgctg gggatccatc gccaccgcct cacgcggcga gataccgaag aattccgcgt 99780cgaaaccgcc cgcgccggac agaaaggcgc cccggcgcac ataactcgta cccgcgctgt 99840ccggatcggg atcgaaaagg ttttccagat cccagccgcg gtcctccgga aactcggtca 99900gcccttcgcc tccggccgcc accaactgcc acaagtcgtc cggattatcg accccgccgg 99960gaaagcggca cgccattccg acgatcgcga tcggctcggg ttccgtggac tccgcttcgc 100020gcagacgccg acgagtgtca cgcagttccg ccgtgaccca cttcaggtgg tcgagaagct 100080tcgcctcgtc cttcgccatc gctggcgcct cctcttcgcg gggccgagat aagatcattc 100140ttgacgggtt ctcttaagct gtcctaaccg acctcccgct ttcaccagga gtggttcaga 100200aggtgcagct caactctttg aagccctcaa tgaagctcga tgccagccgg accggctcgc 100260cctccacgcg gatgtccggc agtgaggcga ggagttcccg gagcatggcg atgatctcga 100320tccgggcgag atgagcaccg aggcagaagt gcggccccac cgcgccgaac gacagatgcg 100380ggttggggtc ccgtgtgatg tcgaaccggt acgcatcctc gaagaccttc tcgtcgtggt 100440tggcggacca gtagaagagg aagatctcgt cgcccttgcg gaaccgatgg ccgttcatct 100500cgcagtcgcc ggtcgcggtc cggcgcatcc agttgatggg cgtgcccacc cggaggatct 100560cctcgacggc gcccttcgcg tggagttcga agtccgacag caggagttgc cgctgatcag 100620gatgttcggt cagcagaacc agggcctggg cgatgacgtt gcgggtcgtc tccatgcccg 100680cgttgatgag gaggatgaag aacgagacca gttcctggtt ggtcagttgc tctccgtctt 100740cctgcacctg gaccagcttg gtgatcacat cggggccggg acgggcgagc cggtcctcgc 100800ggagccgtcc gatgtagtct ccgaggtctc gcagggctcc cagcaccgcc gccgccatct 100860tctccgggtc ggccgcgagt tcgggatcag cccctcccat gatcgtgttc gtccgctcga 100920agaggaattc gtagtcttcc cccggaattc ccatcatggt gctgaggacg gcgatcggca 100980tctccgccgc cgggcggatg aagtcgccgg ggccgcgctc gatgagctcg tccacgatcc 101040gccgggccac ccgccgggac atggcgtcga acttgggcgc cattcctcgg ccgaacgacc 101100gcgcgacgat gcgccgtaat ctggagtgct ccggattgtc catgttgatc atggagccat 101160agaactcgtc catctccggc ggcaggatcg ccgtggcgcc ctccgaggag aagtcctgag 101220ggcggcggct cacctcgcag atgtcggcgt gtttcgacag cgcgtaataa ccggatgcca 101280acggccccca gggcagccgc ggcggaacga attccgggcc cggcagcgcg cgcagcttct 101340cgaacacctc ggcccgctca tcacgcggcc gcagccagaa ggacggatcc atgaagtcgg 101400gaggcctggt ggctttggcg gacgatgcgg gggtggcttc cgtaggagac acaacgcctc 101460gattcaagct cggacaggta caaaaccggt ccgcgcaagt ctcgcaaccg cctcttaaga 101520gttcctaaca caacggcagg ggacgccctg tttgacgccg gtggaacggg ctgtcaacct 101580cggggcgtgc aggtgacgat tcgggacgtc gcgaaggcgt cgggggtgtc cccgtccacc 101640gtgtcgcggg ccctggcgcc cggcggggcg gtgagccccg tgacgcgcga gcgggtacgg 101700gccgccgccg accggctcgg ctaccagccc aaccaggcgg cgcgcgggct gatcaccggg 101760cgtacgggcc atctgggcgt gatcgtcccg gatctgctga atcccttctt cgcggacatc 101820tgcaaggggg tgcaggcgcg ggcccggggg cttggcctca cggtgttcgt gagcgacacc 101880gagcgggacg agggcctgga gctggacgcc atccgcacac tggccccgca ggtggacggg 101940atcgtgctgt gttcaccgca tctgagcggc gaggagctgg ggtcgctcgg ggacttcacc 102000gacaagccga tcgtgctgct gcaccgcaag gagcccgggt tcggaagcgt cacggccgat 102060ctggtggagg ggatgaccga cgcactgacc catctgcacg cgctgggcca ccgcaggatc 102120gcgtatgtcg gcgggccgcg cagctcgtgg gcggcgcggg agcgggcggc cggtgtcgaa 102180gcggtggccg cgtcggggtt ggtggagatc gtccaggtgg ggagcgtggc gccgcatttc 102240gacggcgggg tgaccggggc cgccgatgtg gtgctggcca gcggggcgag tgcggtgctc 102300gcgttcgacg acatcgtcgc gttcgggctg atcagccggt tcacggtgcg cggggtgcgg 102360gtgccggagg agatgagcgt ggtggggtgt gacgatatcg ccctgtcggg gatggcggcg 102420ccgccgctga ccacggtctc ggtgcccaag gcgcatggcg cgcgggccgc cgtcgatctg 102480ctgtgccgca tactggccac cccggccgcc gagggcgagc agcctccgca gcgggtgctc 102540cccacccatc tggtggtacg gggctcgacg gccgccctcg accggcggca gcgggcgtga 102600tggatccact cgccggtctc ctggacgggc cgagggcccg gggggcgttt ctgctccgga 102660tggtgatgga cccgccctgg tcggtacgga tcgaggaccg ggccccgctg tgcgtgatga 102720ccgtggcgcg cggtgaggcg tgggtggtcc cggaccgggg cgaagcgcgg cgccttggcc 102780cgggggatgt ggcggtcgtg cgcggccccg atccgtacac cgtggccggc gatccggcca 102840ccgagcccca ggcgtggatc ctgccgggcg aggtgtgccg caccgccggg ggcgaggatc 102900tggcggagcg gatggcgctg ggcgtacgca cctggggcaa cagcgaacac ggtgcgacga 102960cgatgctggt cggcacgtac cggatggacg gggagatcag ccggcggctg ctggacgcgc 103020tgcccccgct gctggtgctg gggcgtgagc gttgcgattc gccgttgctg ccgtggctcg 103080gcgaggagat cgtcaaggag gaggcgggcc agacggccgt gctggaccgg ctgctggatc 103140tgctgctgat ctcggtgctg cgggcgtggt tcgcgcggcc tgaggcgcgg gccccggcct 103200ggtaccgggc gctgggcgac ccggtggtcg gccgcgcgct gcggctgctg cagaacaacc 103260cgggccatcc gtggacggtg gcgctgctgg cggcggagac cgggatctcc cgtgcggtgc 103320tggcccgccg gttcaccgag ctggtcggcg agccgccgat ggcgtatctg accggctggc 103380ggctggatct ggccgccgat ctgctgcggg agccggacgc caccctcggg gcggtggccc 103440ggcgggtcgg ctacggcagc tccttcgcgc tgagcgccgc cttcaagcgg gtgcgcgggg 103500tcagcccgcg ggagcaccgc tccgcggcca gcgcagggtg acggcggggc cgcgagcgca 103560gccgtcgggc ccgcagctga cggagcgttc gtacagcagg gccagccggc gggcggtgcg 103620gatgatgtcg tagcgctcga cagcggcggg caccgggtgc cgtacggggc cggcggcggc 103680ccgctcgcgc agggcgtcgg ccagggcggc ggtgccgggc ccgatgcggc gggcgccggg 103740ggccgcgccg ggcggcagtt cgtcgagggc cgggcagacg ccgtgagaac cccgggagcc 103800cggccgccag ggcttccagg gcggcgagcc cgaacgcctc ctccgcggac ggcgcgacga 103860gcacatccat ggcggcaagc agcccgggga tgtccgcggg gcggtccccg gtcccggcca 103920ccgccccgtc gcgctcgccg agcagccgga tccggtcggc caccccgagc caggcggcca 103980ggtcgcgcag cgcggtgcgt tcgggtccgt ccccgaccaa caacagccgg gcctggggca 104040gttcggcgac ggcccgcagc gccacatcga agcgcttggc cgccaccagc cggccgaccc 104100cgccgacgac gaaggcgtgt gcggggatgc cgagacgggc ccgggcggcg gtgcgggcgg 104160ccggggtgaa gctgaagtgg cgggcgtcca gcccgttggg caccaggtgg atgcggtggt 104220tggcgacgcc ccagccgcgc agccggtcgg cgacggcggc cgacacggcg acggtcgccg 104280agcccagtcg ttcggtggcg agatagcggg cgcgggtgcc gacggtgagc gggcggccgt 104340cgatatggcg ctcacccagg ctgtgctcgg tggcgaccac ggcgccgaca cccgccagcc 104400gggccgcgac ccggccgtgg acacaggccc ggtagagatg ggtgtgcacc aggtcgtagc 104460cgccaccgcg gatgaaccgc gccagccggg ccgtccccat cgggtcgcgg acacccatcg 104520gcagatgggt gacgggcaca ccgtccgccc ggatgccgtg gccggcggcc ccgggcgcgg 104580tcagggtgag gacgtcacaa cgggcgggca ggtggcgcag cagcagccgc agttgctgtt 104640cggcgccacc cgcgccgagg cgcgagatga tgtgcagcac tctcatcggg cgaccgggcc 104700ccatcttccg tatatcgcac gctgaatcac atccgaccat gacagacggc gggatgtaaa 104760tcacgtaaga cgcgcggcga taggccgtcg gagtggcggc ccgatcggca tatcggcgag 104820gcgcgagggg gcgcgccgaa aatccgattt gcacagccat tccccagcgg attgttacct 104880tctcatctcg cgcgatgaca tgacgaatgt aggccgtccg ggtcatcgcc gcgcgatatt 104940gcgtcccgac gacccgcggg agtgcccaga tgtccgcacc tacccccgtc agagacacgg 105000ccgagacggg agccgcgccc agcaccgctc cccctcccgc gcggcccgcg caccatgaga 105060tggccccctg ccgccggggc ccgaccgaga gttatgagta ccagcggtac agccagctgg 105120cgggcccgct gacccagccc ccgtccggcc gcccgtaccg ggtgcgctgc cggagcctgc 105180tggcgcagga accgcaccgc gtccgcgcgg cgctgctgct gtgcgcggcg ccggtgtgct 105240cggcgctgct gctgttgtgg ctgctgcagc cccagcactg ggtgcaccgc gacggtgtga 105300cgggctggga gacggccgcg gaccggacga tgctgttctc gatcgcgctg atcgaggcgt 105360tccgcctgtt caccgtggtc tccaacgcgc acgccacgct ggtggcccgc gacccggtgc 105420cggtgaccgc cgagcccggg acccgggtgg cgttcctgac cagcttcgtc cccggcaagg 105480agccgatcga catggtccgg gcgacgctgg agggggcgat gcaggtcaga cacgccgggg 105540ggctcgacgt atggctgctc gacgagggaa acgacgagga ggccaaggag ctgtgcgcac 105600ggctgggcgt acggcatttc tcccgcaagg gcatcgagaa atggaatcag cccaagggct 105660ccttccgcgc caggaccaag cacgggaatt acaacgcctg gctggacgcc catggcgacg 105720actatgaatt catggcctgt gtggacaccg atcatgtgcc gctgcccaac ttcctggagc 105780gcatgatggg ttacttccgc gaccccgatg tcgccttcgt ggtcggtccg caggtgtacg 105840gaaattacga tacggcggtc accaaggcgg ccgagagcca gcagttcctc ttccacgcgc 105900tgatccagcg ggccggaaac gcctatggcg gcccgatgtt cgtcgggacc aacaacgcgg 105960tgcgcatcgc cgcgctgcgg agcatcggcg ggctgtacga ctcgatcacc gaggacatgg 106020ccaccggctt cgagctgcac cgccgccgca accccgtcac ccggaagaag tggcgctcgg 106080tctacacccc ggacgtactg gccgtcggag agggcccgag cacctggacg gacttcttca 106140cccagcagct gcgctggagc cgcggcacct acgagacggt gctcaagcag ttctggaagg 106200ggccgttcac gctgtcgccg gggaagctgc tcaactactc cctgatgatc gcctactacc 106260cgatgaccgc gctcaactgg atgctctctg ccctgtcctg cgcgctgttc ctggtgctcg 106320gcgcgtccgg gatccatatc ccggcggaga tgtggatgat gctctacagc gacgccgcgg 106380cgctccagat cggcctgtac atctggaacc ggcggcacaa cgtctccccg cacgaaccgg 106440agggctccgg cggggtggcg gggatgctgc tctccgcgct gtccgccccg atctacgcca 106500agtcgctgtg cgacgcgctg ctgcggcgca agagccggtt cgtggtcacg cccaagggcg 106560actcctccag cgccgaccgg ctgtccacct tccgtatcca tctggtgtgg gccgcggtct 106620tcggcggctc gctggtcgcc tcgctgttcc tgggccacac ccacgccgcg atgcgcacct 106680gggcgatgct ggcgctggcg ctgtgtctgg cgccgatggc catctggtcc ggcgggctgc 106740tgctcacacg gcgcggacgg cgcaccgccc cggcggtgcc gacggcccgc gccgaggccc 106800actgaagctt gtggtcacct ggcgcgcaac ctggcgttac gggccaggtc gagcgcgtac 106860tccgtccacc aacgacctgc gggcggaccg ccgttgcagg tcccgtcgga ctctcccgga 106920cgcttgatcc acagatacgc gtcgatggcg gggtcgcccg tacgggtgct ggggggatcc 106980cccagagccc gccccggcgg gttgcaccag ctctcctggc cgtcgctggc ggagcgcacg 107040gcgcccatgt caccgctctt gccgtcgtcg ccctcgcccc tgacggaggt gagcgggccg 107100ttgccgttgc ggctggtgtc gatgatgaag tggaccctgc cgagctggag ggacagccga 107160cggccgtagg cgcgggtcac cgctgtcggc tggaagttcg agacgttcag ggcgaagccg 107220tccgcctggt cgataccgct tttcatcagc ggctccacca gccgctcggt gtccttgatc 107280caccccgggt tcccggcgtc cagatagatc cgggtggcgg gcatcgcgga gagcatggcg 107340accgcgtcgc gcagcagggc cagccgcggc gcacgcagcg gcttgggaat gcagccgtcg 107400accacctgag cgatcgcgtc gggctccaat acgacaatcg cccttcgctc gccgattccg 107460gagacgatct cacggatcca gtccagatat tcggcgtcgc tgctcgcccc gccccgggag 107520tatcttccgc agtcccggta cggaatgtga taggcgacca gcaccggcgt acggtgctcc 107580cgcgctgcgg attcggtgat aaaccggacc cgttcgcgcg gatgcgggcc gagccattcg 107640gcgatcggct cgtcggcgat ccgccgcatg agctccgcct ccgcgtaacg gcccgcgcgc 107700cgctgctcac gggcctgccg ggcggccggg ttgtccgggt caacccatag cccgctccgc 107760cggtccgacc cgcctgacgt gccgagacat ccgctcagcg cgaggagcgc ggcggcacat 107820cccgcaagcg gcgcccaatg tcgcgcggtg gctctcccga gccctttctc ggccattcga 107880gcacaccttt tccccggagt ttcccggcct cggaatcgac ggcacgtgtc gcatcatggc 107940atcgggaacg ggcggcggct ttcaattcac gcttgtgttg ccccgccgcc atcggggcga 108000atttcggaaa gggccgtgaa acttacccgt cgcggtcctc gtcggtccgc cagagcgggt 108060gttcgcgggc cgcccaccta cggtcgaccg tcccggtgcg cagaccgcgc cgggcctccg 108120ggtcgccgag cgccatgccg atgtgcccga gcagaacgat gccgacggcc agcgccagcc 108180agtcgtggac gaacgtggcg ctgatacgcc agaccagggg ggccagatcg gtgaaccaca 108240tcagcagccc ggtacccgcc atcaccagca cggcgccgct gatataggcg gcgtacagct 108300tctgccccgc gttgaacttg cccgccgggc gcgccccctc gcgcgtgtcg cgccgcagca 108360ccgcgcgcag ccagcggcgg tcgtgcgggc cgaagcggtt gaggcgggtc aggtcggcgc 108420ggaaggcgcg ggagaccagg cccagcagcg tcgggaccgg ggtcaggatg ccggtccact 108480cgtgcaccgt gaccaccagg gcgcggcggc ccacgagttc ggcgagctgc ggcagataca 108540gacaggccgc gctcaccaca caggtgccca ggaggacggc ggtggtgcgg tggatccacc 108600gctcggccgg ggtgaaccgg gccacgcggg ggcgggggcc ggtgcgggcg cgtgcggcgg 108660ggcgtacggg ggcgggacca ccggggtgcg cgtcaggtcg tggcggcatc gtcgcgtccg 108720ttcgacttgc cgacccaggc gtccacgtca tagccgcggt cctcccaata gccgggctgt 108780acctcgtggg tgacctcgat accggagagc cacttcgccg acttgtagaa gtacatgggg 108840gcgacgtaca gccgcaccgg gccgccgtgg gagtggccca gcggctcgtc ccgcatccgc 108900agggcgacca ggacgtcgtc ccggcgggcc tgttcgaggg tcaggctctc gctgtacgcc 108960ccgtcgaagc aggtgaagcg gacggccccg gcccccgggc ggaccccggc ggcgtccagc 109020agcgtggcca ggcgtacgcc ctcgaaggag gtctgcggca cgcgccagcc ggtgacgcac 109080tggacgtcgt ggacgatgcg gtgctggggc agcgcgcgga ggtcggcgag ccggtagccg 109140gtgggtttgt cgacaagtcc gccgacggtc agccggtagt tctcggcgtc ttgtgcggga 109200cggaggaggt caccgagtag tagcggaacc cgccgccgtt ggggagcagc ccggtcaccc 109260cgatggggtc gttctccgcc gccacggcca ggaacttctc caggcggcgc tgcagccagg 109320gcgcggcggc catgccgccc gcgcccaggg cgagcatgcc gaggatgacg cgccggccga 109380cgggggtgcc gcgaccggcg gcgtccgggt cgggggtatc cgagccgggc gtgtccaggt 109440cggcggccgg gcctggttct gtggcacccg ggtcagcggt gtccggatcg gcggcggggt 109500gctccgcggc gccgggattg cggtccatgt ccctgatgag agcactccgg gggcggcggg 109560cgccaggctc ttcggccttt cgtcagactt ccgtcagact tccgtcagaa gctggcgcac 109620ccgctctgcc ccaccggccg ccctacccca ccgccttcgc cgccgcgcgg cccgccgtac 109680ggccggagaa gagacagccg ccgaggaagg tgccctccag ggagcggtag ccgtggacgc 109740ccccgccgcc gaacccggcc gcctcgcccg ccgcgtacac accgggcagc ggctccccgc 109800cctcggccag cacccgcgcc gacaggtcgg tctccaggcc gcccagcgtc ttacgggtca 109860ggatgttgag gcgcacggcg atcagcgggc ccgccttggg gtcgaggatg cggtgcgggg 109920cggcggtgcg gatgaggcgg tcggcgaggt acttgcgggt gccgcgcagg gccgtgatct 109980ggaggtcctt ggtgtagggg ttggcgatct cccggtcgcg ggcgacgatt tggcggcgca 110040gctcggcctc gtcgatcagc ggttccttgg tgagttcgtt catccgccgt acgaggccgg 110100ggagcgatcg ctcgacgatg aagtccgcgc cgtggtccat gaacgcctgg accgggcccg 110160gggcgccgcc ccgcccgcgg ttcagcacat cgcggacgct cttgccggtc aggtccgggt 110220tctgctcgga gccggagagc gcgaactcct tctcgatgat cttccgggtg aggacgaacc 110280aggtgtagtc gtagccggtc cgcatgatgt gttcgagcgt gccgagggtg tcgaaaccgg 110340ggaacagcgg cacgggaagc cgtctgccgc gggcgtcgaa ccacagcgag gaggggccgg 110400gcaggatgcg gatggcgtgc cggggccaga tggggttcca gttctcgatg ccctcggtgt 110460agtgccacat ccggtcgcgg ttgatcagcc gcgcgcccgc cgcctccgcg acgcccagca 110520tcttgccgtc cacatgcgcg gggacgccgg agagcatccg ctcgggcggg gtgccaagac 110580gctcgggcca gttctggcgg acgagttcgt ggttgccgcc gatcccgccg gaggtgatga 110640tcaccgcttg ggcgcgcagt tcgaaggcgc cggtgacctc gcggctgctg ggggcgccgc 110700gctcggcgtc gctgggctcc aggatctcgc cggtgacggt gtcgacggcg cccgcgctac 110760gggacagtcc ggtcacccgg tgccggaagc gcagctggac caggccgcgg gcgaccgcgg 110820cccgcacccg ccgctcgaag ggcgcgacga cgccggggcc ggtgccccag gtgacatgga 110880agcgggggac ggagttgccg tgcccggtcg cgtcatagcc gccgcgctcg gcccagccga 110940cgagcgggaa cagccgcagc ccctgggcgt gcagccaggc gcgcttctcc ccggccgcga 111000agtccacgta cgcctcggcc cagcggcgcg gccagtggtc ctcctcacgg tcgaacccgg 111060ccgtgcccag ccagtcctgc caggccagtt cgcggctgtc gcggatgcgc agccggcgct 111120gctcgggcga gtcgacgagg aagaggccgc cgaaggacca gtgggcctgg ccgccgaagg 111180attgttcggg ctcctggtcc agcaggatca ccttgcggcc cgcgtcggcg agttcggcgg 111240tggccgccag gcccgcgagc cccgccccga ccacgatcac atccgcgtcg tacgccatgg 111300tttctggtgt cccgttctgt caggagcgcg caggaaggcc ggtggccggg ccgtggtgcc 111360cgggcaccgg tggtgtcggt cgatcctgcc taccggccgg tatggcgtca acccgccggg 111420cgggcagcgc cccgctacgg gcttcacggc gcccggcagc gggcccccaa ctcccgcccg 111480gcgcgccgac ctgtgtgacg taacgccaat aaaggggaca atcgatcgcc gagatggaca 111540gcgggttcgg cgggcgggca tcatcaagca ccatggctga ccaccatcct tctccctcgc 111600gaccgcgcag cgcggacaag cgtgtggtga gtcctacgct ggtcactccc ggccctcagc 111660ccgtcgagct cgccgggtcc tgcgccgagg acgaaccgca ggtgctgcgg ggcgaggtgg 111720tcggcccccg gcacgcccgc cgacgccccg tcagcgaccg gcgcaaggcc gcggccgggg 111780cgattctgct ctccgccacc ggcgccgcca ccgcgctctt cctgatgctg ggcaaggacg 111840gccgccaggc cgccgccgcg cccgcacacg acgcgccgtc gtcggcgccc gacgcgagcg 111900cggacgacgc cgtcccggtg gccgacgcgg tggccggcgc caagccgctg ccgggcgcgt 111960cgcgcaccgc cgagcccgtg gcggccccca gcgtcccgac cgcacagccc aaggccccgt 112020ccacggcgcc caccaagccc ccggcgcgca cccagcagcc gacgcgcccc tcctggccgt 112080cccagtgggg aggccagggg cggaccgccg aggactggca gcgggaccgg gaggaggcgg 112140cccgctgggc gaaggagtgg gcggaccggt acggctccgg ccaagacggg tccggccatg 112200acgggtccgg ccgcgggggg agccaccagg gcgggtcggg ctacccgggg tacccgggcg 112260gagggcacgg aggacagtgg cgctgaccgc gccccgtact gcttgggccg ccgttctgct 112320tggatggcgg catgtctgac aaggaaccgc cgcaggagcc gcgttccgcc gatgagctgc 112380tggacatcgt ggacgagcgg gatcgggtgg tgggccaggc ccggcgagcc gacgccatgg 112440cccgccggct gcgccatcgg accgtcttcg tgctgacccg ggacggtgcg gaccggatct 112500tcgtacatcg ccgcaccgcc accaaactcg tcttcccttc cctgtacgac atgttcgtcg 112560gcggtgtcgt cggcgcgggc gagtcgtacg acacggcggc gctgcgggag gccgaggagg 112620agctgggggt atccgggctg ccggcgccga cgccgctgtt ctccttcctc tacgacacgc 112680ccgagcacac ctggtggtcg cggatctacg aggtccgctg cgagctgccg gtcgccccgc 112740aggccgagga gatcgcctgg cacgccttcc tggacgagga ggagctgggg cggcggctgg 112800cggagtggga gtgggtgccg gacggcgcgg aggcatatcg gcggctgctg gagtggcgcc 112860accagggccg gtctccggca ggtccggagg gtcctaacgg ctttccccgc gattagcccc 112920ttgccatggc catgcgtgcc ggccggcacc gtgcccacgg ggaaggtgac cgcctcgggc 112980aagccaggga tacggaaggc cacctggtgg gaccacaccg gccccctcgc cgtcgtcttc 113040gcgggcggcc ccgacagcaa cgccggtgac ctcgtgctgc ctgtgcgtat cccgcagggg 113100cccgggcagt gggcgcgagt ggaacacttc ctcgcaggcc ccggccgctg gcacaaggtg 113160gaccttgtcc gccgacgcaa agccagtgcg ccgggcggct gggtgtatga ggcgcacctg 113220atgattctcg gccccggcta caccgccccg gcggtacagc ggatgcggca gcgggccgcc 113280gccctggacc ggatcggcgg ggtggacggc aacgtctcca acctctcgat cgtctccttc 113340cccgccggcc tggaccccgc cgagggcgcc cccgcgtcca ccgagatcac cctcaccgac 113400gccgagcgcg ccctgttgga gagacaggcg aagaagcggc gcggccgtgc ccgtgcgctg 113460gaacgctccc gccgggccac gaacaccgcc cagtacgggc tgtcgaagaa gcagacccgc 113520cgcgctgagc acagggccgc gaagggcttg cccgtcaaga ccgtgacggt tccaggcggt 113580gcccgcgccg cccgtacgga cggggttccc aagcaggcat tccgccgcga ccggctgtcc 113640gagggctacc ggaatctgcg cgcccgccag gccgaagccg ccgccagcgc cgccgaacac 113700cgccgccacc gagcccgcgc cgtggcccgg gagatcatcg ccgcccacgg cgtgaacctc 113760acggtcgagg actgcgacat ccgcacctgg taccggctgt ggggcaagca cctctcccag 113820accacccctg gcatgctgat cgccgccctg gaccgggaat gccgggcagc gggcggacga 113880ctcgtccgcg cctccacctg gtcaaccgcc ctttcccagc actgcctgtg cggccagcgg 113940gtcaacaaga cgctacgcga ccgcgaacac aagtgcgtcg cctgcggcct ggtcggcaag 114000cgtgacctcg tatccgcagc cctggccgcg ttcgtccgcc tcaccgacgt ggacgacccc 114060aagaccgcgc acctgcacaa cgccatgtcc cggcacgcac agatcacata cggacaaggg 114120ctggaagagg ccctgcgtga gtcaactaca ccgaacccga aaccggttcg ccggccggga 114180cgcgtggcag tcccccacca gcgggagacc tctgctcacc gaaccgccgc aaggcggccc 114240cgagcaaccc cggatgagac acgccccgcg cgtgaccacg ccggaaagcc cggacgccgc 114300cccggctgcg acccgcagct cgccctctgg tgaattgcgg aacaagtctt agtaggcttg 114360tcccatgatc gacgccaacc gcacgccgac ccggggcagg ctcgcctccg ccgtcgcgga 114420tgtgcggctg tggttcgcgc ccgagcggct gcgcgacgag ggggagaccc ccgactaccg 114480tttctccctc gccaacgaac gcaccttcct ggcctggctg cgcaccgcga tggcgctggt 114540gggcgggggc ttcgcggtcg atcagttcct gccggacacg aacagcgcgc tgcggctcgc 114600ggcggcgctc acgctgctgg cggggggcgc ggtgtgcgcc gtgcgggcgc tcaaccactg 114660ggtccgctgt gagcgggcga tgcgccgcgg cgaggacctg ccggtctccc gcttcccggt 114720cgtgctgggg ctggccgtcg gggtgatcgc cctggtgatg gtgggcctgg tcgcggtccg 114780ctggggccgc tgagccgatg gccgggcccg actcgcccac ggggccgcag gacgaccccg 114840agcgacccga tccccggtca cccgaatcgc ccgatccggg gtccgagggt ggtccctgga 114900agcgggaccc cgggctgcag ccggagcgga cccggctggc ctggcggcgt acgacactgg 114960cctgtgccgt cgccgcgtcg ctggccgcac ggcaggccct gcacaacggg accgggccgg 115020tgtccgtgct ctgcgcggcg ctggccgcgc tggcctggct cggcttcctg ggtgtggcgg 115080gccgccgcat ccgggcggtg tccgcgccgc gtccgcccgc actgtccccg cgggcggccg 115140cggcggcggc aggctgcacc ctggcgctcg tggccttcgg cgccgcgctg acccgatgac 115200gcgctgatct gccgaagccg gcccctaccc gaccgtgacg acgatcttgc cgcgggtgtg 115260gccctcctcg ttgaggcggt gcgcgtcggc ggtcttctcc agggggaaga cggacgagac 115320ctccacgccg agggcgccgc gctcggcgat gtcggtgagg tcctggaggt cgttcgggtc 115380gggccgtacg aagacatagc ggccgccgag ggcgaggaca gtctggtcgg tgatggaggc 115440cagccgcccg cccggggcca gcagcgggac ggattcggtg agcgcttcgc cgccgacggt 115500gtcgaagacg gcgttcaccc ctccgggggc cagcgtccgc acccggtcgc ggagcccgtc 115560cccgtaagtg atcggctcgg cgcccagctg gcgcaggtac tcgtggttgc gctcgctggc 115620ggtgccgatg acgcgggcgc cgaggtgccg ggccagctgg acggccatgg agccgacgcc 115680gcccgcggcg gcgtgcacca aaacggtgtc gccctcggcc acctccaaca ccttgaccag 115740cgtctggtac gccgtgagac ccgccagggg aacgcccgcg gcctgcgtga acgtgagatt 115800ccgcggcttg cgggcgagcg tacgcaaggg ggctgcgacg tattcggcga aggtgccccg 115860ggacaggaag tcctcccgga cgtatccgat cacctcgtcg cccgccgtga actcggtcac 115920cgccgggccg gtccgttcga cgacgcccgc gacgtcccag cccgggatga ccgggaagac 115980cgcctccagg acggagtcca ggtagcccgc ccgggccttc cagtcgaccg ggttgacggc 116040cgcggccctc accttgacca ggacggtgtc ggggccgacc ttgggctcgc cgacctcccc 116100gtactccagg acctcggggc cgccgtagcg gcggtaggtg atggccttca tgggaggctc 116160cttgcgttcc tgcgcggtgt gagggccgga cggcgagcgc ggccggagcc cgcgctcccg 116220tcttcgacgc tcccgcgccc gccgacccgg cgcaaaccgg gcgggctagc ccacgctgaa 116280gttgcggcag gcggagctgt cggacctctc gacggcactg cttccgacag ggatcgcctg 116340gttacccacg ccgcccttca tcggactgcc tttgtcaact actcgtcgat aaatcgatga 116400gcttgcacaa cgggcgtcac tggctgcgac acgtcgcttg cgtccagctc caccggagcg 116460gagcaggggc gattgactgc gccccgcacc tatgcagtcg aacgtcccgt gtgaaaccgg 116520gtccaccggc aaaaccggat ctgtgccttc ggagactccc gcatgggagt agcaaaatac 116580cgcaatctgc gcagcgttga ccagctccgc cggccagagg ccgcgaacga ctcagcactg 116640gactccatac cgactggtcg gcatcatgat gcgggaccgt ccgaccaccc gtcccgcgcc 116700cgcccgcccg cagcaccctc aacggaggca gagacccgat gaactcaggc aatccgccag 116760gactcgacct cgaacggctc cgcgcccatc tggaccgtga gcggccgggg ctggtgcgcg 116820ggccgctgcg tgccaagctg atcgagggcg ggcggtcgaa tctcacctat acggtggacg 116880acagcgccgc ccgctgggtc gtgcgccgcc caccgctggg ccatgtgctg gccaccgccc 116940acgacatgcg gcgcgaacac cgggtgatcg gcgcgctgca cccgaccagc gtgcccgtgc 117000cggagccggt gctgctgtgc gaggacgatt cggtcctcgg atcgccgttc tacgtcatgg 117060agttcgtcga gggcacgccg taccgcacgg ccgaacagct caccccgctc ggcccggagc 117120gcacccgccg tgtcgtgctc aacctggtgg acaccctggt cgagctgcac gccgtcgacc 117180cggccgccgt cggtctcgcc gacttcgggc gcccggaggg gttcctggag cggcagctgc 117240gccgctgggg caagcagctg gccgcctccc gcaaccgtga gctgcccgga atcgacgagc 117300tgcacgaagc gctcggcaag gcgctgcccc tcgccgcttc ttctggcgcc cccgccgtgg 117360tccatggcga ctaccggctg gacaacgtgc tcgtgggcgc cgatgaccag atcaaggcaa 117420ttctcgactg ggagatgtcc acactcggcg atccgctgac cgacctgggc ctgctggtga 117480tgtacagcga gcagcaggag acgtccgact cgccgatcac cacgaccagc ggcgcccccg 117540gccaccccgc gccgagcgag ctgatcgagc ggtacgcggc cggctcgggc cgcgatgtcg 117600gcggcgtcgc ctggtacacg gcgttcgcgt acttcaagct cgccgtgatt ctggaaggca 117660tccactaccg cttcaccctc ggccagaccg tcggcgccgg cttcgaccgc atcggcgaag 117720tcgtcccggt gttcgtcgag agcggcctca ccacgctacg gaagggctga gacagccatg 117780gacttcgcat tcgacgcccg aaccgaggag ctgcgggcca agctgctcgc cttcatggcg 117840gagcacgtcc acccggccga ggcggtggcc gaggagcagc gcgcccggct ggactccccc 117900tggctgaccc cgccggtcgt ccaggagctc aaggcggagg cgcgcaagca gggcctgtgg 117960aatctgtttc tgcccgacgc cgagctgggc gcggggctga ccaatctcca gtacgccccg 118020ctcgccgaga tcaccggcca cagcccccag ctggccccca cggccctgaa ctgcgccgcc 118080cccgacaccg ggaacatgga ggtgctggcc cagttcggca gcgagcagca gcgcaagcag 118140tggctggagc cgctgctcgc gggcgagatc cgctcggcgt tcgcgatgac cgagcccgag 118200gtcgcctcgt ccgacgcgac caacatcgag acccggatcg agcgggacgg cgacgactac 118260gtcatcaccg gccgcaagtg gtacatctcc ggggcgatga acccggactg caggatattc 118320atcgtcatgg gcaagaccga tccggacggc cccgacctcc gccgtcagca gtccatggtg 118380ctggtggagc gcgagaccga gggcgtcgag gtgcgccggg ccatgcgggt ctacggctac 118440gaggaccact atcacggcgg ccacgccgag gtggtcttcc acggtgtgcg cgtcccggcc 118500gccaatctga tcggggagga gggcggcggc ttcgccatcg cccaggcccg gctgggcccc 118560ggccgtatcc accactgcat gcggctgatc ggcatggccg agcgcgcgat cgagctgatg 118620tgccgccggg cggtgtcccg tacggccttc ggcaagccgc tcgcccggca gggccaggtc 118680cacgcgtgga tcgcggacgc gcgggtggcc gtcgagcagc tgcggctgct ggtgctcaag 118740acggcctggc tgatggacac cgtcggcaac caggcggcgc acaccgagat ccaggccatc 118800aagatcgcca ccccgcgcac ggtcgtcgac atcctggacc aggcggtgca gctgcacggc 118860gcgggcgggg tcagccagga cttcccgctg gccgagctgt gggccgcggc gcggacgctg 118920cggctcgcgg acggaccgga cgaggtgcac cagcggtcgc tggcgcggcg ggagctgaag 118980aagtacctct aaaaccccct gaaaaccgct gtcacggccg cagggcgcgc agcagcaggt 119040cggcgaggtg gtcggcgacc tgctgggggc tgagcggtcc gtcggggcgg taccaggtgc 119100ccaggtggtg gatcgagccg aagtggtagt ccaccaccag gtcggccggg gtgtccgagc 119160tgaacacccc ggcccgctgg ccctcctcca ccagggcgcg gaaccgctcg tggtagcgcc 119220gccgctccgc ccgtacctgc ttgtgcttct ccgggctgag atggtgcatg gaccggaaga 119280agatcgtcgc gtcgtcgagg ttgtcgatgg tggtgacgac gacgtcggcc gccgcgtcgc 119340gcagccgctg ctcgacgggc gcgtccgcgt ccgcgaaggc gtccaggcgc tcctgctgca 119400gccgtagcac ccgtccgtag atctcgtgca gcagatcgtc cttggagccg aagtagtggt 119460agagggcgcc cttggtgacc ccggccgcct cgacgatctc ctggacggag gtccggtcgt 119520agccgcgctc cgcgaagagg cgggtggccg cggccagcaa gcgctgtggc accggtttcc 119580cgtcaccgtc cctcatcctg gccatctcgt cgccacctgc ctctcgttgg tgccgggttc 119640gctactcccg ggggctccgc gactcccgca gctcccgtct gaggatcttg ccactggccg 119700tcttgggcag ctcgggcagg atctctatcc ggcgcggata cttgtacgcc gcgagccgct 119760gcgcgcagta cgaggtcagc tcctccggcg ccacctcggc gcccggccgc aggctgatgt 119820acgccttgac cgtctccccg cggtacgggt cggggacgcc gacgacggcc gcctcgcgca 119880cggcggggtg ggtgtacagc acatcctcca cctcgcgcgg ccacaccttg aagcccgagg 119940cgttgatcat gtccttcttg cggtcgacga cgtagagcca gccgtccggg tccatgaaac 120000cgatgtcccc ggtgcgcagc tccccgtcgg gcagggccgc cgcgctcgcc tccggtgccc 120060gccagtagcc gggcaccacc atggggccgc gcaccgcgat ctcgcccggc tcgccgaacg 120120gcacgtcccg gccctcgtcg tcgatgatgc gcaccacgga gtcggggccc ggcaggccga 120180ccgccagagt gcccgagacc gggtcgacgg gggcccgctt accgggcggg acgctggcgc 120240agggggcggt gcactcggtc aggccgtagc cgttcttgag gtagaggccg aagtccgcct 120300cgaaccgctc gaccagcgcg ggcggcaccg gggccccgcc cgaggaggcg agcaggaagg 120360acgcgaagtg gtcgcgggtc accttcgggt gtgccctcag cgccatgaag gcggtggagg 120420ggccgacggt gtacgcgggg cggtgctcgg cgaaggcatc gaggacgaca cccggctcga 120480agcggtaggc catggcgagg gtcccggcgt ccgcgatgga ggtggacagc tcgcagacca 120540tgccggtgat gtggaacagc ggcgcgagca cgaagatcgt ggacccgtcg ggcagctcgt 120600gaccgatatg ttgccgctcg gcgttgtagg cgatgttgcc gtgggtgttc agggcgccct 120660tgggggtgcc gctggtcccc gaggtgtagc tgatcagcgc gatgtcgtcg ggtcctggct 120720ccggcacgtc cggccgcgcc gcgccgccgc gggccgccgc gagcagatcg tcggtgtcct 120780cgggcgcggg gatgcgccca tgggtgagga cgcgggggtc gtcgcgggtc tggaagtcgt 120840acgcgtcggc ggtgagcgcg atgcgtacgg aggaggcggc ggcggccgtt tcgcgcgcgt 120900actcctccca ggcgtggccc gagcacacca gggcgctgac ctcggcgtcg gccaggatgt 120960gaccgagctc gccccccttg tacatggggt tgaccggcac caccgtgccg cccgccttcc 121020acgcgccgag cagggcgagc acgaagtgcg gggtgttctg caacatgatc gcgagccggt 121080cgccgggcct gaagccccgc gcggcgagat gcccggcgat gccgtcggag agctcatcgg 121140cctcccggta ggtcacctgc ccgtcgaagt aggcgaacgc ggcccggtcg ggcctggtgc 121200gcacggcgtc ccggaaggcg tgcagcacgc tgggccgggg ccggatcggt tggcgctgca 121260cctcggtgag ctgagctagc catggctggt cctggtatga ggtcatgagg gcggccctgt 121320ctgctcggga gatgggggtg cgggggcgta cggatcagcc ggtggccccc attaacggct 121380cgtttcgccg gcttcgacct tgcgctggag gtggttcatg tgcgtgagcc agcggtcggt 121440gtcggtggcg cgggccgcgt agtactcggc gacctcgggg tgcggaagga tcaggaagcg 121500ctcgtgcgcg atcccgtcga agagggcgtc ggcgacctgc tcgggctcga tcgccgtcgg 121560aacgaggatg aggtcgccgg tggggccggt ggcgcgcagc atatcggtgc ggacgccctg 121620cgggcagatc gcgtggacgc tgacgccgcg atggcggtag gtcgtggaca gccactcggc 121680gaaggcgagc gcggcgtgct tggacacgct gtacggggcc gagccgacca tggtcagcag 121740accggcggcg gagacggtgg agacgaagcg gccgctgccg cgctccagcc actcgggcag 121800cagctcacgg gccgcccgga catgcgccat gacgttgacg tcccaggcgt ccgcccacag 121860cagctcgtcg gcctcggggc cgccgatggg ggcgacaccg gcgttggcgc agtagatgtc 121920gatgcccccg ccgagcgccg cccgcgcctc ggggaccaca tgggaggcat cgcccggcac 121980ggccaccgcg ccgatctcgt cggcgacggc cttcgccttg tcggcgtcga tgtcgttgac 122040cacgacccgg gcgccctcgg cggcgaagcg gcgggccagg gcggcaccga tgccgcctcc 122100cgccccggtg accacggttc gcgctccacg tacggcgtct ccacgcacgg tatccacgca 122160tgctccttcg gccgggggtc ggctgcgtcg ctcagccgcg tcgctcagct gctcggcaga 122220ctaaccagtc ggtatgtgta aacggaaggg gcacgacggg gtacggaagg gaagcgccgg 122280ggtgccgcgc ggacggggcg gccggatcca cttccggccg ccgggctcac ctcatagggc 122340gaagcgagcg gtccgcggtc gtgacggctc cgaaggtttc tccggcaccc cgtggccacg 122400tggcgtcgag gagaccgtct ctgtgcttac tcagcaaggc ttactcagca agcagggcca 122460cggtctcctg tagccgctcg cacgcccagt cgtactgctc ggtgccggtg tgtgcccgga 122520agttcgtctc gatgatctgg atcagagcga ggtgaaggtt gtacagccgc cgacgggtcc 122580gctcggcggt ggtgaacggc cggtggccgt agccgcgaat gaaagccgtg gcgtccccgt 122640aggcgctcga ctcactcccg gcgaagccga actccatcag cgggtcaccg tagaacgccc 122700gctcgtggtc gattactgcc acgatccggc cgtcgcccac catgcagttg ccgggccaca 122760ggtcccactc gacgaaccgg ggctcggtca cctcgtccaa cgaatcggcg tgagcggcca 122820cgacttcgcg gatcacgtcg tagccgtggg gcaggtcgac gccgcgtcgc tcgccgtcgc 122880gcagcaactc ctcgaccatc cgcaggaagg ccgcgcgcca cgtgctttcg cccggcccgg 122940ccagcgggcc gaaggcggtg ccggggatgg tgttgagttc gcgggtgatc gcgccgagcg 123000cctcgtcgta cgcgttgatc tccgttccgg ccacggtgtc cctgatggcg tggaggttgt 123060ccgcatctat gtacgtcatg aagaagtagt cggcatcgca cacctcatgg ctctgatcgg 123120cgaagtcgac ctctggcact ggaaccttcg tgtgctcccg gatcaaccgc agcgccgcca 123180gttcggtggc catggcgccg cgctcgtagg tcatgacctc gacatcgggc ggcggtgcga 123240tcttgaggac agcttgcgta ccggatcgca gccggatccg gtaggccacg ttgaaccagc 123300cgtcgctcag ttcgctgacc cagtcctcgc ccgcgtcggg cacctcctcc gggccgtagg 123360cgcgggcgac catagcgcga agcgcctcaa cgggctgccg gttctttgtg atgctctcca 123420tcaaccctcc acccgaagca tgatcggacg gtagcaccag gacggagttg tgcactcggg 123480tctcctgcga tgccttgcta ctgctgcggg cgggcaactg gggctgccgc agccggttcc 123540aggtcgcgtc ctgttgggcg cggaggccgc gcccaggagc ccgcggctgg tttcggtggc 123600gttcggggtc gggccgtcat cagggaagat ccgcggcgat acgcacggtg gttgaaccga 123660tcaggcgcgt ggttttctgc tggtcgtgaa gagcacctcc aagcactcga aaacgcttga 123720ggccgcgccg gccacggccg aggatgccgt gggccggatg gcgacccgcc cggtgggcag 123780gctgctgtgg gagaactgcg tacagaccat cgcgtccgtc agcatgttcg gcttctacgc 123840cctgacgaac gcctggttcg tcagccacgg ggtcggcaat caggcgatgg ccgcggtcaa 123900cctcgtcgcc ccgctgctgc tcctgctcgg agccatgtcc accaccgtgg gcgcgggcgg 123960cgcgacgctg ctctcccgcg cgctgggcgc cggcgacgag cgcgccgccg cccgcgcggc 124020gggcaacgcc ttcacgctgt tctgggtcac cgcggccgtc accaccgtgg ccgggctggt 124080cttcatcgac ccgctcctcg acgtgctggg cgcgcgcggc tcactgcgcg agtacgcccg 124140gcagtacgcg gtgatcctgc tgtgcgggtc agtgtcctac accgggttct ccagcctggt 124200gcgcgccgag ggccgggtcg ggttctccac tcggatgtgg atcggtgccc tcgtggtgca 124260gatcgccctc gacccgctgc tgatcttcgg cctcgacctc ggggtgagcg gagctgccct 124320gggcaccgtc ggcgggcaga ccatatccgc cgtgatgagc tggtggttct tcttccttcg 124380ccgcgagcgg ccgtaccgcg tccgcctcgc cgacctgcgc ccacacgccg tgaccctgag 124440cacgctggtg ggcatcgggc tgccgtcctt ctttgccaat atcggcctca ccgtgctcgc 124500cgtactggtc aacagcaccc tcgcggtgac cgggggagtg atagcgctga ccgcgtacgt 124560cgtctgctcc cgcctgcaga ccttcgccgt gatgccgcac acaggcatga gccaggggct 124620gcagcccatt gtcagctaca acgccgggcg cggcctggac gcccgagtag cgcgaacccg 124680ggcgctcgcg ctgcgggcct cgctcgtcta cggcgcggcc agcgcgatcc tgctgacggt 124740cctcgccgaa ccgctcgtcg gcatcttcgt cagcggcggc ggagccgcgg aggaggccag 124800gttcgcgctg tgcatcatcg ctgtcggcgg gatcgtcacg ggtatcgccc cgctgaccgc 124860ggcctatttc caggctctgg gacgcccgct gcccgcctac gtcctgacca tcggcaccct 124920cgcatcctgc tgaaggcgcc gctcgtcgtg gcgttcggcc tgatctgggg cacgcacggc 124980gtatggttcg gcctcgccgc cggcgagttc gccaccgcgg cgagcgcgct gctgctgttg 125040caccgcgccg accggcgcgc gccgccccgc ggcaccggat ccccccgctg cgtcctgaga 125100cgtccgatga cgcgacgggc gaaaccggga ctccggccgc aggacaccga ccggcgaccg 125160cgagcgagcc cgccggtcac gacggcccgg tcggttgccc gttactgaga cacgctccta 125220gctgccgctc tccccgccgc ccgagccctc cccggcctcg cccgggttct tcatcacgct 125280ccgccagcgg gcgttctcgc ccgccagggc gtcccacagg acgttgagcg ggtgggcggc 125340gtcccatgcg gtgcgctccc caccgcgtac gaacaccccg accagcacgg ccagatccgg 125400cccgaggtca tccgccagct ggagcgtccg cagccccgca ccgctctgca gccgcccgtc 125460ggcgaccgct tcgccgatcc cctgggtcac cagcacacag ccgcgcagca cgcccgagga 125520gagcagttcc aggccgtact gcacggtgtc gatctcggcc gcgatatgca gctcctggcg 125580gtagcgggcg ccgaaccagc cgcgcagaaa gccgggtatc aggccgtcgg cggagaccgc 125640gagcggcagc ccggggagtt cgcggaccga cagcccgggg cccggcagtt cgtcctcgga 125700gaggttggtc accagggaga gcccgctgcg ccgccactcc atcacctcga agccgtccag 125760ccggctgtcc ccgtcctcgg tggtcagcac ggagccgcag accaggtcga gttccttgga 125820gccgagccgg tccagcagat cgcgggtgcg cacatgctcg accttgagct ccacaccacg 125880gctctcgaag tcctcggtca ccagttccac ggcgcccagc agaaagccga gggtgtagcg 125940ggtggagccc acgttcaacc ggctgccgag ccggcggcgg cagccgtgca ccgagtccag 126000ccagtcctgc agcgtgccgc gggcgagttt ggccagggct tccccggtgg gggtgaagag 126060cacgttacgg ctccggccgc gcttgagtac gagcggttca ccgcacagcg cgccgaagtt 126120acggttcatg gtgtcgagtt gtttctgaac gctggactgt tcccgcccca gcagccgggc 126180cgcgcccagt gcggtacccg cctcgtggac cgcgagcagc gtgcggagct ggtccatcgt 126240ggtgtcgagc agcgccgtgg ggtactgcca ccgggggttt aagggcatac cggccttccg 126300ctccgagcct acgaattccg gcttcgcagc atagtccaat gggctccggc aaactgatcc 126360ggagaacagc ggcaattttc ttgcgatttg tattcgggaa ttcgacagac tctattccac 126420aggggcgatt ccggatttcc cgtcgctgtc ggcggcgctc ggctgggccg cggggcttgc 126480cagtgccacg cgctacacgc tggccgcggc ctcccgggac gcgagccgga ggtgcggcgg 126540gtcggcgtgc tgtacacgct cgcctcccag ctggccgggg acagggcctc gctgcggggc 126600ggcccgatcc gcgctgggga caacggggta cggcgtcagg cccggcccga taccggcccc 126660cctcatgccc gagtgaccga gcactacatc tccgattgaa gctcagtcag gttttctcaa 126720cggtgatcag ttccatatcc cgtgaagacg agggcttcgt tgccgctcac gagtcggtcg 126780atggttcacg tcgggcggcc cgtcaggagg cttccgggcc gtgagcgcac ggagggacaa 126840cgtcgccgat cacgctgacg cgcgtccaga gcccgtcacc gtcgtcgagg tcaccgcaca 126900agtggaccgc cggccatccc cgggccagcg ccgggcgccc gtccccgtcc aggctgtcgc 126960ccacaacgag gcacacggcc tgatcgcggg tgaacgcgtc gctaacggcc tggaaaaacc 127020gcggatgcgg cttggcatag ccgacctccg aggagacgaa caccctggcg acgtgctgtt 127080ccagaccgct gaggctcagc ttgagtcgtt gcaggcggga ggccccgttc gtgatcaacc 127140agacctcgtg gttgcgagcc attcgctcca cgaaccccgc cgcccccggg aagggccgca 127200ccagcgcctg ccgatgccgc gcgaactgcc gcgtggcatc ctcacgatcc gcgtctgcgc 127260ccgctgttcg cagcacctcg ccccagacct ccacgcggta tcgctgcccc cacgtggccg 127320cctgcggcac cgacgcgagc gtcagatccg cccaaagcgc ctcccaagag ctgatcccca 127380ggtctctcag gcgctcgaag tacggccctg agcgccaatg agaccgggcg acatcgaaca 127440cgctctgcac ggtctccgaa ggacgcgcta ctccgaccgc gtgcagagtg ccctccacgg 127500ccgcccaggc ggcgcttcgg tccgggagca gtgtgtcgtc caggtccacg aggacgacga 127560acctacttgt ctccacacaa cctcccgctg acaccgcgca ttgcagccga cctggcggcg 127620gacaagcgcg aagcccctgg taggacaggc ctcaccacaa gatcatgtcc gtaattacca 127680gaggcttcac gttggtcacc tatgttgcca cgctcgacgt accgcgccat gtcgtcgagt 127740tcctcgccag cctgtcggcc gcccaccgac ggcggatcgg cacgccgaag ggctcccgcg 127800cgctcgggcc gtttcgtcag gcagtagctg gtgctgcgct ggtcccgcga gcgggactgc 127860gtgcactgcc tggcccgcga cgccggcgtt tcccaggcca ccggctaccg ctaccttcac 127920gagggcatcg acgtcctcgg cgaccagacc ccgtgggtcc accgcagccc cagtactcca 127980cttggaagta ccggggctgc ggcgttgcat gatcagctca gtttcgtatg ccacttccct 128040cgggcgagga ttgtcgttcc gtccggcttg atgtacacct cggcgccgcg cacgggaagc 128100tcaccctgcg tgagccagta ggtacggcac tcgtcgagca ggtcccacag gcgtcgcggc 128160ccgccctggt gcacggttgg ccgatcgtcg ccgaccgcgg ttgcacgggc ccacgaacca 128220tcctcgtgtg ccatgagtgc gacgcgtcgc tcgccgtcgt cctcatacga atgctcgata 128280ccgggggcct tgaggctcag catcgaatcg aggtcccacg cgttcgccac gtcgacgatc 128340ggataagggc cgagcgctac ctcgtcgccg tcctgctccg cggccttggt gagcacatcg 128400ccgacaccgg gcgggtagtc atcgccggcc cgggcgtgca tgaaccctgc ccggtcccac 128460tcgacgcgcc ccgaagcgct cccgtcccgg ttcttctccg cagtgatgag cagagaggtg 128520ccggcgaggg tggtgacgag tcgccccccg gggcgtagca ccgtgagcca actcgcggga 128580accgcgcgca cggcgaccgt ggcgacgatc cggtcgaaca tcgacgcggc gaatggaagc 128640tcccccgtcg cgtcgacggc ctcgacggtg gggccgacgc ctgcctcggc gagacgcgag 128700cgcgcggcct cgacgaggta ggggtcaaca tcgacgctcg tcacctgctc gtcgctgagg 128760cgccgggcgg cgacggcggc cccgtacccg cttcccgtgc ccacgtcgag caggtagtcg 128820ccgtcatcca tgcgggcgtg ctggaacatg cgcaccacca ggctcggcag ggttgccgag 128880gaggtcggcc tgccgtgcgg ccggtcgctc ggggtggcct gatccgcatg caggggcccg 128940acacgggtca cgagcgacgt gtcgcggtac gccgattcca tccagcggtc cgggtctgcg 129000ggcccgtcga gcagttccca gccgtcggaa ccgcgccccc accatcgcgg gatgaacagg 129060tgccgcggtg tggcggcgac ggccggcgcc catcgggagc gctggtgtgt tgcaacgcct 129120gccagggcgg cggcgtggtg gggccagttc acgcgtggac tccaagagag ggggctgaca 129180gctgctgagt aatccgtcag tgcggctcag cctctctcgg cttgtgtcgt gatggcgtga 129240acctcgccga ctgggacgtg cgtcagggcg tgccctgccc ggtatggggc cgggtctttg 129300cctccctgga tggatacggg tgttccttca tcgaatgcgt gacctttgtg tgtggtgttc 129360gttgggtgcg gtgaggggat cttcgaaccg ggggaggcag tgacgtgcgg gggaagttcg 129420ggccgcggtc cggctgtgtg gtgcaggcct acaggttcgc tctcgatccg aacgccgggc 129480aagggcgggc gttgcgttcg cactgcggtg ccgcccgcgc cgcctataac tgggccgtga 129540cctgggtgac cgcctcgtgg tggcagcgca aagccgaagc cacctacggc atcggcgagg 129600aggagctcac cccgtggcgg ccgtggtcgc tgcccgcgct gcgcaaggag ttcaaccgga 129660tcaagaccac cgacccgaga ttcgcccagt ggtgggagga gaactccaag gaggcgtaca 129720gcaccggcct cgcgaacgcg gccgccgcgt tcgacaacta cgccaagtcc aagcagggca 129780aacgcaaggg gcgccggatc ggtgtcccgc gccggaagcc gaagcggaag gcccgcctgg 129840cctgccggtt caccaccggc acgatccgcc tcgagccgga cggccgacac ctcaccctgc 129900cccggctggg cacaatccgc acccatgaac ccacccacaa gctcctcacc cgcatccagg 129960ccggcacggc gcgcctcctg tccgcgaccg tccggcatga gcgcgggcgc tggttcgtct 130020ccctccaggt ggagacagca cgggagatca tccgcgttgc ccggccggat gtggcggtgg 130080ggatcgatct gggtgtcaag cacctggcgg tcctggccga cagttgtgga cagacacggt 130140atgagccgaa cccgaagcac ctggacggcg ccctcaggct gctgaggctc cactcccgcc 130200gcgtctgccg gcggcagggg cctgaccgca ggaccggccg gaagccgtcc aggcggtggg 130260agagggccaa ccgcgagcgc aacaggctcc accaccgggt ggcgaacctg cgcgccaatg 130320cgctgcacaa gttcaccacc cgtgtgcgcg ccgagtacgg cacggtggtg gtcgaagacc 130380tcaacgtcgc cggaatgctc cgcaacaaac gactcgcccg ccacgtggcc gatgccgggt 130440tcggggagat ccgccgccag ctcacctaca agggccaacg aaacgcctgc cccaccattg 130500tggcggaccg ctggtacccc agctccaaga cctgctcgaa ctgcggcgcg gtgaaagcca 130560agctgccgct gcacgtccga gtcttcacct gcgacgcctg cggcctggtc ctggaccggg 130620acgagaacgc agggcacaac ctggtcgccc tcgtggctgc ctgcaccact ggtaccggag 130680tggccggaga ccaggacacg ccaggcgtgt cgaagccccg tggagccgac cgtaagaccc 130740gccgtcaacg ccccgaccgg aacaccggcc gaggcgggcg ggcaggtggc gcaagcccgc 130800cgcacccgcg gcggaaggaa acggggaccg tcgtcaggac accgagcgca acccacgctc 130860cggtgacacc gtcatggacc tttcagacgg aaacgtctgg aatgctgaga gtcactgaga 130920ccttgagtaa cggaactcca cgacctgctg gtacgtcggc cggttctgcc agtgcacctt 130980gtcgtgggtg acaccgccca gcgggcgctg gatgatcgag tcggcgcacc actggtcgcc 131040cgccgagcag ttggcgtcgc cggggtagac ctccgtggcg ggtttggccg cggcctgctt 131100gaggctggtc aggagggcgt cacggcacgc cgacaggtcg ccggcgccgc agtacttcct 131160ggcgagcggg ccctggacat cctcgccgag gaccgcgcgc aggtccttgt cgacgtagga 131220ccaccagccg tactggaagg aggacccggc atgcgacccg gtgggcccat ggccggccga 131280gggcgcctcg tcgacggtca gattggcgcg cagcgcatcg tacagatcgg ctccgagctg 131340aggtttgaac tcggcctcca ccagaagcgg ccaccaagcg tccatgatcc gtacggcgtc 131400ggcgtgtcca taggtgtgcg aaccggcgga tgtctcacgg cgctgggcgc ccgccgtctg 131460ccaggactcc agctgctgca cggccttggc cgcttgcgga tcggtgaccg gcgcgctgcg 131520caggaccttc agcagctccg gcagcacgtc ctcgccgcgc aggtcggtga cggcggcctc 131580ggccatggcg cgggtgaggg ccgcgcgggt caccccgccc ttcttcacca gggcgcgcac 131640ccggtcgtcg aggaggttgc cgcggtgcac cgagccgttg ccgaagccgg ccgcggtgta 131700gcccttggcc tgccggttgt tccagctgac gtagtagtcc tgatcgacgg actgggggtg 131760ctgggcggcc ggggcgtagg cggcggtgtt gaccgtcggg tcgaagtccc gccactcgta 131820cgccgcctcg cccttgatcg gaagcgaggg gtccacgtcc gccgcccgga ccaggttcag 131880cccgctgttg tagtaggcgg tctgccggga gtcggcgtag aaccagttga aggtgtagtt 131940gatgtgctgc gcggcgctct ggaaggactt ggcgtccttc acatagccgg ggtcgttgag 132000catctggaag ccgacgatgg agtcggcctc gtggcggtag ctggcgcgca gcagggtgta 132060ggcgacgggt ttgccgtcga ccgtcgcgcg atgggtcaca agaccgtact tggtgcggta 132120gacctgcatc cggtacgagc cttcggcggt ggagtcggcg agcgtcggct tccaggcgtt 132180gcggcgctcg atcttctcca tcggcaggca ggagccgtgg tagcggtagt acgtcgagtc 132240cttcgtgggg gcagacccgt ccggcgtgca cagctcgacg gcgaaggtgt cggtgacgtc 132300ctgggcggaa gtggtggcgc tccaggcgta gtcctggccg cggcccagct ggacgtacat 132360ccccacgccc gcgaaggagg cgccgcgggc gctgatgccc ggaccctgga gctcctggag 132420catcagcagc tgcggggcga aatagccggt ctgcgggccg aagacggcga cgggatggcc 132480gctcgcggtg tatttgccgg agaccaggag cgcgttggac atcccgtgct tacggctgaa 132540caga 132544<210>2<211>456<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met His Asp Thr Thr Lys Asp Asp Pro Arg Ala Ser Glu Arg Arg Ser1 5 10 15Gly Val Arg Arg Ala Phe Val Ala Ser Leu Thr Gly Thr Ala Leu Glu20 25 30Trp Tyr Asp Phe Ala Val Tyr Ser Ala Ala Ala Ala Leu Val Phe Gly35 40 45Asp Leu Phe Phe Pro Ser Glu Asp Pro Leu Thr Gly Thr Leu Leu Ala50 55 60Phe Ser Thr Tyr Ala Val Gly Tyr Val Ser Arg Pro Leu Gly Gly Ile65 70 75 80Val Phe Gly Arg Leu Gly Asp Val Ile Gly Arg Lys Lys Val Leu Ile85 90 95Ala Thr Leu Val Leu Ile Gly Ala Ala Thr Phe Leu Ile Gly Val Leu100 105 110Pro Thr Tyr Ser Thr Ile Gly Val Ala Ala Pro Ile Ala Leu Val Val115 120 125Leu Arg Phe Ala Gln Gly Val Gly Val Gly Gly Glu Trp Gly Gly Ala130 135 140Val Leu Leu Ser Ser Glu Phe Gly Asp Ser Arg Arg Arg Gly Phe Tyr145 150 155 160Ala Ser Ala Ala Gln Val Gly Pro Pro Ala Gly Asn Leu Leu Ala Asn165 170 175Gly Val Leu Ala Ala Leu Gly Ala Leu Leu Thr Glu Ala Gln Phe Glu180 185 190Ala Trp Gly Trp Arg Val Ala Phe Leu Leu Ser Gly Ala Leu Val Ala195 200 205Phe Gly Leu Trp Ile Arg Ala Lys Leu Glu Glu Thr Pro Val Phe Lys210 215 220Ala Met Glu Ala Glu Gln Ser Arg Pro Glu Ala Pro Ile Arg Glu Val225 230 235 240Phe Thr Thr Gln Pro Arg Ala Leu Leu Ala Ala Ile Leu Cys Arg Val245 250 255Gly Pro Asp Val Leu Tyr Ala Met Phe Thr Val Phe Val Leu Thr Tyr260 265 270Ala Thr Gly Glu Leu Gly Met Ser Arg Gly Ser Ala Leu Ala Ala Val275 280 285Leu Ile Gly Ser Ser Leu Gln Val Phe Leu Met Pro Leu Ala Gly Ala290 295 300Val Ser Asp Arg Ile Asn Arg Arg Val Leu Tyr Gly Cys Ala Ala Val305 310 315 320Ala Ala Gly Val Trp Pro Phe Leu Phe Phe Pro Met Ile Gly Gly Gly325 330 335Ser Trp Ile Pro Leu Ala Leu Gly Val Val Val Gly Leu Val Ile His340 345 350Ser Phe Leu Tyr Gly Pro Gln Ala Ala Phe Ile Ala Glu Gln Phe Ser355 360 365Pro Arg Leu Arg Tyr Thr Gly Ser Ser Leu Ala Tyr Thr Leu Ala Gly370 375 380Ile Ile Gly Gly Ala Ile Ala Pro Leu Leu Phe Thr Thr Leu Leu Ser385 390 395 400Ala Tyr Gly Ser Trp Leu Pro Leu Ala Leu Tyr Ile Ala Val Ala Ala405 410 415Ala Val Ser Leu Ala Gly Val Leu Leu Gly Arg Asp Pro Glu Ala Ala420 425 430Val Asp Glu Asp Ala Gln Leu Ala Thr Pro Ala Ala Gln Ala Thr Ala435 440 445Asp Ala Ala Arg Pro Thr Ala Val450 455<210>3<211>271<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Leu Leu Gly Gly His Arg Pro Ser Gly Ile Leu Leu Val Tyr Arg Ser1 5 10 15Tyr Ala Gln Ala Leu Arg Lys Lys Leu Thr Ala Glu Lys Ser Thr Thr20 25 30Ala Asp Arg His Pro Asn Ser Gly Ser Ala Pro Met Ala Arg Leu Thr35 40 45Phe Glu Leu Pro Asp Gly Ser Thr Arg Glu Val Asp Ile Val Gln Val50 55 60Leu Asn Ala Gly Tyr Ala Gly Arg Ser Gln Asp Asp Val Ala Ala His65 70 75 80Ile Ala Glu Leu Ala Glu Leu Gly Val Pro Thr Pro Ser Val Thr Pro85 90 95Ala Leu Tyr Pro Val Ala Pro Tyr Leu Ala Gln Gln Ile Asp Arg Val100 105 110Ala Val Gln His Arg Arg Thr Ser Gly Glu Ala Glu Trp Ala Leu Val115 120 125Val Ala Gly Asp Gly Glu Leu Leu Leu Thr Ala Ala Cys Asp His Thr130 135 140Asp Arg Asp Leu Glu Val His Gly Val Ala Trp Ser Lys Asn Ala Gly145 150 155 160Pro Asp Val Leu Ala Arg Arg Ala Trp Arg Leu Ala Asp Val Glu Pro165 170 175Arg Leu Asp Asp Leu Thr Leu Arg Ala Trp Val Thr Arg Asp Gly Thr180 185 190Glu Thr Glu Ile Gln His Gly Thr Leu Ala Glu Leu Leu Thr Pro Ala195 200 205Tyr Trp Val Asp Val Leu Arg Ser Arg Asp Ala Leu Thr Pro Gly Thr
210 215 220Val Leu Ile Ser Gly Thr Ile Pro Met Thr Pro Gly Val Asp Gln Phe225 230 235 240Ala Asp Thr Trp Arg Val Glu Leu Gly Asp Pro Ala Thr Gly Asp Thr245 250 255Ile Arg Leu Ala Tyr Asp Val His Pro Met Pro Glu Pro Ile Gly260 265 270<210>4<211>233<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Leu Ser Thr Arg Cys Thr Gly Ser Leu Thr Asn Gly Trp Val Ser Val1 5 10 15Glu Leu Gly Ile Pro Leu Ser Val Val Leu Val Asp Asp His Pro Val20 25 30Val Arg Ala Gly Ile Ser Ala Trp Cys Ala Ala Ala Asp Ala Pro Ile35 40 45Ser Val Val Ala Glu Gly Ala Asn Val Ser Val Ala Leu His Gly Pro50 55 60Gly Arg Gly Ala Asp Val Val Val Met Asp Leu Leu Leu Gln Asn Gly65 70 75 80Arg Pro Ala Tyr Asp Glu Leu Gln Glu Leu Val Ala Gln Glu Arg Lys
85 90 95Val Val Val Tyr Thr Met Arg Asp Ser Gln Asp Ala Ala Leu Thr Cys100 105 110Met Asp Leu Gly Ser Ala Thr Tyr Ile Thr Lys Ala Glu Gly Gln Arg115 120 125His Leu Val Lys Ala Ile Arg Ala Ala Ala Glu Asp Ile Pro Tyr Thr130 135 140Pro Pro Ser Leu Ala Gly Ala Phe Gly Ser Asp Thr Arg Gln Ser Arg145 150 155 160Pro Val Leu Ser Val Arg Glu Val Glu Val Leu Val Glu Trp Phe Gln165 170 175Ser Glu Ser Lys Ala Val Val Ala Gln Ser Leu Gly Ile Ser Glu Arg180 185 190Thr Val Asn Thr Tyr Leu Asp Arg Val Arg Ile Lys Tyr Ala Asn Ala195 200 205Gly Arg Pro Ala Thr Thr Lys Ala Lys Leu Val Ala Arg Ala Val Gln210 215 220Asp Gly Leu Ile Ala Leu Asp Glu Leu225 230<210>5<211>399<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Val Gly Glu Asp Phe Arg Gln Leu Ile Thr Arg His Gln Leu Arg Gly1 5 10 15Ile Arg Ala Gly Gly Leu Val Ile Leu Val Thr Leu His Ile Gly Ser20 25 30Ala Leu Pro Gly Leu Leu Gly Gly Leu Gly Glu Tyr Arg His Pro Trp35 40 45Ile Pro Leu Ala Ala Tyr Gly Leu Leu Thr Leu Ile Leu Gly Gly Ser50 55 60Val Val Leu Gly Leu Asp Gly Arg Pro Trp Pro Arg Gly Trp Val Pro65 70 75 80Cys Ala Leu Ala Gly Thr Phe Val Ala Ser Val Ala Thr Thr Ser Gln85 90 95Val Pro Ala Asp His Tyr Phe Val Ser Ser Leu His Trp Ser Tyr Ser100 105 110Leu Ala Gly Trp Phe Thr Val Val Leu Leu Gly His Arg Gly Pro Leu115 120 125Val Ser Gly Ala Phe Leu Gly Ala His Leu Ala Val Thr Ala Ala Leu130 135 140Leu Leu Thr Val Asp Val Pro Ser Arg Ser Ile Gly Ala Ser Met Ala145 150 155 160Leu Ser Ala Leu Ser Ala Gly Cys Phe Gln Met Thr Ile Ala Ala Glyl65 170 175Ala Lys Leu Leu Leu Asp Ser Ser Ala Ala Ile Gly Gln Ala Leu Arg180 185 190Ala Gln Glu Arg Val Arg Thr Arg Ile Ala Val Ala Arg Gln Ile Gln195 200 205Ala Asp Gln Arg Arg Arg Tyr Ala Glu Leu Asn Ala Thr Val Leu Pro210 215 220Leu Leu Thr Gly Leu Ala Ser Gly Ser Leu Asp Pro Glu Asp Asp Glu225 230 235 240Val Arg His Ala Cys Ala Leu Glu Ala Ala Arg Leu Arg Arg Leu Phe245 250 255Ala Glu Ser Asp Cys Thr Phe Asp Pro Leu Val His Glu Met Arg Ala260 265 270Cys Ile Asp Val Ala Glu Arg Asn Gly Thr Thr Val Gln Leu Ala Val275 280 285Arg Gly Asp Pro Leu Glu Leu Pro Leu Pro Leu Arg Arg Ala Leu Leu290 295 300Asp Pro Val Ile Ala Ala Leu Ala Ala Ala Gly Gln Thr Ala Arg Val305 310 315 320Thr Val Val Arg Gly Gly Asp Gln Val Arg Val Gly Val Val Val Asp325 330 335Ala Leu Arg Asp Arg Leu Pro Glu Pro Lys Ala Asn GIy Ile Arg Val340 345 350Arg Thr Val His Ala Glu Asp Arg Leu Leu Val Glu Ala Ala Cys Arg355 360 365Ile Thr Ala Arg Pro Glu Leu Ser Asp Arg Pro Glu Pro Pro Trp Pro370 375 380Pro Ala Trp Pro Ser Trp Ser Pro Ala Gly Arg Arg Ser Arg Thr385 390 395<210>6<211>396<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met Ser Ile Ser Ser Leu Ser Pro Gly Ile Asp Pro Pro Ala Ser Ala1 5 10 15Arg Leu Pro Gly Ser Ala Gly Pro Trp Ala Arg Glu Tyr Val Ala Phe20 25 30Asn Arg Arg Leu Ala Leu Tyr Gly Arg Ala Gly Val Met Ser Ala Cys35 40 45Ala Val Leu Gly Val Ala Ala Met Pro Met Gly Glu Met Ala Pro Ala50 55 60Ala Val Val Ala Ala Ala Val Leu Ala Trp Ser Trp Ile His Leu Arg65 70 75 80Leu Ala Thr Thr Ser Ala Met Arg Gln Arg Pro Leu Leu Ala Leu Asp85 90 95Leu Thr Val Met Thr Ala Leu Cys Leu Ser Gln Arg Phe Thr Ile Pro100 105 110Glu Ala Gln Thr Thr His Ala Gly Thr Trp Ile Leu Val Ser Val Ser115 120 125Phe Thr Ala Val Ala Tyr Gln Leu Met Gln Pro Pro Leu Thr Gly Ile130 135 140Leu Ala Thr Leu Trp Leu Cys Cys Ala Asp Val Val Gly Thr Ala Leu145 150 155 160His Pro Asn Leu Glu Trp Ser Gly Val Leu Arg Ser Val Ile Trp Val165 170 175Val Val Asn Thr Ala Leu Ala Arg Ala Val Val Arg Leu Val Phe Ser180 185 190Glu Ser Arg Ala Ala Asp Glu Ala Ala Asp Arg Ala Ala Arg Ala Arg195 200 205Gln Gln Gly Glu Ala Ala Glu Ala Arg Cys Ala Ala Glu Arg Glu His210 215 220Leu Ala Ala Leu His Asp Thr Ala Cys Ala Thr Leu Leu Ile Ala Ser225 230 235 240Ala Pro Trp Ala Ser Met Arg Ala Glu Thr Leu Arg Ala Gln Ala Ala245 250 255Arg Asp Leu Leu Arg Leu Arg Ala Glu Gln Pro Val Ala Gly Glu Val260 265 270Asp Leu Ala Ala Glu Leu Leu Asp Glu Ile Ala Ala His Pro Leu Arg275 280 285Val Ile Asn Arg Phe Asp Val Glu Leu Gly Thr Thr Trp Arg Pro Ile290 295 300Ala Ala Ala Leu Arg Gly Gly Leu Gly Glu Ala Leu Arg Asn Val Ala305 310 315 320Arg Tyr Ala Gly Val Asp Ala Val Arg Val Thr Ala Glu Arg Val Asp325 330 335His Val Ile Val Val Thr Ile Ala Asp Ser Gly Val Gly Phe Asp Pro340 345 350Glu His Ile Pro Gly His Arg Ile Gly Ile Lys Arg Ser Ile His Gly355 360 365Arg Met Trp Asn Val Gly Gly Arg Ala Thr Val Glu Ser Arg Pro Gly370 375 380His Gly Thr Thr Val Arg Leu Glu Trp Pro Arg Gly385 390 395<210>7<211>241<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Leu Gly Pro Leu Arg Val Val Asp Glu Lys Gly Gly Ser Pro Leu Arg1 5 10 15Ala Gln Lys Leu Ala Ala Val Leu Ala Val Leu Leu Ile Arg Ser Asp20 25 30Gln Val Val Met Ala Glu Gln Leu Thr Arg Glu Ile Trp Gly Glu Leu35 40 45Pro Pro Arg Arg Asp Thr Ala Ser Ile Tyr Val Cys Ile Ser Arg Leu50 55 60Arg Lys Phe Leu Ser Arg Pro Gly Gln Gln Asn Pro Ile Ile Thr Arg65 70 75 80Pro Gly Gly Tyr Met Leu Arg Arg Ala Ser Ser Glu Cys Asp Phe Asp85 90 95Val Phe Gln Gln Leu Val Ala Asp Gly Arg Lys Cys Val Arg Tyr Gly100 105 110Glu Pro Ala Gln Ala Ala Leu Cys Phe Glu Gln Ala Leu Ala Leu Trp115 120 125Arg Gly Pro Ala Phe Gly Asn Val Gln Thr Gly Pro Ile Val Ala Gly130 135 140Phe Leu Lys Arg Leu Asp Glu Met Arg Ile Glu Cys Ala Glu Met Ser145 150 155 160Ala Glu Ala His Leu Glu Leu Gly Arg His Arg Glu Leu Val Gly Arg165 170 175Leu Arg Ser Leu Val Ala Glu Phe Pro Leu Arg Glu Thr Phe Tyr Arg180 185 190Gln Leu Met Leu Ala Leu Tyr Arg Ser Asp Gln Lys Ser Glu Ala Leu195 200 205Gly Ile Tyr Glu Ser Ala Arg Arg Val Leu Asp Arg Glu Leu Gly Leu210 215 220Glu Pro Gly Arg Lys Leu Arg Glu Leu Arg His Thr Val Leu Ala Ser225 230 235 240Thr<210>8<211>253<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met Arg Tyr Glu Ile Leu Gly Pro Leu Arg Val Val Asp Arg Ser Ser1 5 10 15Val Thr Ser Ile Ser Ala Arg Lys Ile Glu Val Leu Leu Ala Ala Leu20 25 30Leu Val Arg Ala Asp Glu Val Val Ser Ser Glu Asp Leu Val Lys Glu35 40 45Ile Trp Gly Thr Arg Ala Pro Arg Gln Ala Val Ala Ala Leu His Val50 55 60Tyr Val Ser Gln Leu Arg Lys Phe Leu Arg Arg Pro Asn Arg Thr Glu65 70 75 80Ser Pro Ile Val Thr Ala Arg Ser Gly Tyr Val Leu Arg Leu Gly Asp85 90 95Asp Glu Leu Asp Phe His Val Phe Gln Gly Leu Val Arg Gln Gly Arg100 105 110Ala Ala Gln Arg Ala Gly Arg Thr His Glu Ala Cys Glu Ala Tyr Glu115 120 125Glu Gly Leu Glu Phe Trp Arg Gly Pro Val Leu Glu Asp Leu Arg Asp130 135 140Gly Ala Ile Val Asn Gly Phe Val Cys Trp Ala Glu Gln Ala Arg Leu145 150 155 160Glu Cys Val Glu Ser Phe Val Glu Ala Gly Leu Glu Leu Gly Arg His165 170 175Arg Glu Phe Val Ser Tyr Leu Tyr Ala Arg Ile Glu Glu Leu Pro Leu180 185 190Asn Glu Ala Phe Tyr Arg Gln Leu Met Leu Ala Leu Tyr Arg Ser Gly195 200 205Cys Arg Ala Asp Ala Leu His Val Tyr Gln Ser Ala Arg Ala Val Leu210 215 220Ser Glu Glu Leu Gly Leu Glu Pro Ser Ala Ala Leu Lys Arg Leu His225 230 235 240His Ala Val Leu Val Asp Ala Pro Glu Leu Glu Ala Ala245 250<210>9<211>2902<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Leu Arg Phe Arg Tyr Gly Gly Val Met Ala Gly Ser Ser Pro Ser His1 5 10 15Ala Gln Gln Ala Thr Ser Pro Val Ala Ile Val Gly Leu Ala Cys Arg20 25 30Leu Pro Gly Ala Pro Asp Pro Glu Ala Phe Trp Arg Leu Leu Arg Ala35 40 45Gly Glu Asn Ala Val Val Pro Val Pro Asp Ser Arg Leu Pro Thr Glu50 55 60Pro Gly Ser Pro Pro Tyr Phe Ala Gly Leu Leu Glu His Val Asp Thr65 70 75 80Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Thr Ala Met85 90 95Asp Pro Gln Gln Arg Leu Met Leu Glu Leu Ala Trp Glu Ala Met Glu100 105 110Asp Ala Gly Leu Gly Pro Lys Asn Leu Ala Glu Arg Arg Thr Ala Val115 120 125Phe Thr Gly Ala Ile Trp Asp Asp Tyr Ala Thr Leu Leu His Arg Arg130 135 140Arg Pro Asn Asp Ile Ala Ile Thr Arg His Thr Met Ala Gly Leu His145 150 155 160Arg Gly Leu Ile Ala Asn Arg Val Ser His Leu Leu Gly Leu Arg Gly165 170 175Pro Ser Leu Thr Val Asp Ala Ala Gln Ser Ser Gly Leu Val Ala Val180 185 190His Leu Ala Cys Glu Ser Leu Arg Arg Gly Glu Ala Asp Leu Ala Leu195 200 205Ala Gly Gly Val Asn Leu Ile Leu Ala Glu Glu Ser Met Arg Met Ala210 215 220Glu Ala Gln Phe Gln Gly Leu Ser Pro Asp Gly Arg Cys Tyr Thr Phe225 230 235 240Asp Ala Arg Ala Asn Gly Phe Val Arg Gly Glu Gly Gly Gly Met Val245 250 255Leu Leu Lys Pro Leu Ala Ala Ala Val Ala Asp Gly Asp Pro Val Tyr260 265 270Cys Val Ile Glu Gly Ser Ala Val Asn Asn Asp Gly Ala Thr Asp Gly
275 280 285Leu Thr Arg Pro Ser Ala Asp Ala Gln Thr Asp Val Val Arg Gln Ala290 295 300Trp Gln Arg Ala Ala Val Ser Pro Ala Glu Leu Gln Tyr Val Glu Leu305 310 315 320His Gly Thr Gly Thr Pro Val Gly Asp Pro Ile Glu Ala Ala Ala Leu325 330 335Gly Asp Ala Leu Gly Asp Ala Phe Asp Gly Cys His Arg Asp Arg Ala340 345 350Val Asp Ala Pro Leu Arg Val Gly Ser Val Lys Thr Asn Val Gly His355 360 365Leu Glu Ala Ala Ala Gly Ile Ala Gly Leu Leu Lys Thr Ala Leu Ser370 375 380Ile His His Arg Arg Leu Pro Pro Ser Leu Asn Phe Ala Thr Pro Asn385 390 395 400Pro Gly Ile Pro Leu Ala Glu Leu Gly Leu Arg Val Gln Thr Ala Phe405 410 415Gly Pro Trp Pro Asp Glu Arg Arg Arg Leu Thr Ala Gly Val Ser Ser420 425 430Phe Gly Met Gly Gly Thr Asn Cys His Val Val Leu Ala Glu Pro Pro435 440 445Ala Pro Ala Val Leu Pro Asp Arg Pro Thr Ala Pro Gly Asp Val Ser450 455 460Ala Arg Thr Ala Pro Pro Val Met Pro Trp Val Val Ser Ala Ala Ser465 470 475 480Pro Lys Ala Leu Thr Ala Gln Ala Ala Ala Leu Tyr Glu His Leu Arg485 490 495Ala His Pro Gly Leu His Pro Val Asp Ile Gly His Ala Leu Ala Thr500 505 510Thr Arg Thr Ala Phe Pro His Arg Ala Val Val Leu Gly Arg Asp Glu515 520 525Asp Glu Leu Val Ser Arg Leu Asp Ala Leu Ala Ser Glu Thr Gln Thr530 535 540Ser Gly Val Ile Arg Gly Arg Ala Gly Gly Gly Arg Val Ala Phe Leu545 550 555 560Phe Ser Gly Gln Gly Ser Gln Arg Pro Gly Met Gly Arg Glu Leu Tyr565 570 575Ala Ala Tyr Pro Val Phe Ala Asp Ala Leu Arg Glu Val Cys Ala His580 585 590Leu Asp Pro Met Leu Asp Thr Asp Thr Pro Leu Leu Asp Leu Met Phe595 600 605Ala Glu Ala Pro Pro Asp Gly Glu Pro Pro Leu Asn Arg Thr Ala Tyr610 615 620Thr Gln Pro Ala Leu Phe Ala Ile Glu Val Ala Leu Tyr Arg Leu Val625 630 635 640Thr Ser Trp Gly Val Thr Pro Asp His Leu Met Gly His Ser Val Gly645 650 655Glu Ile Thr Ala Ala His Val Ala Gly Val Leu Ser Leu Pro Asp Ala660 665 670Cys Thr Leu Val Ala Ala Arg Gly Arg Leu Met Gln Ser Ile Thr Ala675 680 685Pro Gly Ala Met Ala Ala Trp Gln Ala Thr Ala Glu Glu Ala Gly Gln690 695 700Ala Leu Glu Ala Tyr Gly Gly Arg Val Gly Leu Ala Ala Val Asn Ala705 710 715 720Pro Ala Ser Val Val Ile Ser Gly Asp Arg Glu Ala Val Ala Glu Ala725 730 735Thr Ala Ala Trp Arg Ala Arg Gly Arg Lys Ala Thr Val Leu Lys Val740 745 750Ser His Ala Phe His Ser Pro His Leu Asp Gly Ile Leu Gly Asp Leu755 760 765Arg Thr Val Ala Ala Gly Leu Thr Phe Ala Ala Pro Ala Ile Pro Val770 775 780Val Ser Asn Leu Thr Gly Gly Ala Ala Thr Glu Ala Gln Leu Arg Ser785 790 795 800Pro Asp Tyr Trp Ala Asp His Ala Arg Gln Ala Val Arg Phe Asp Ala805 810 815Gly Val Arg His Leu Cys Asp Ala Gly Val Asp Thr Phe Leu Glu Leu820 825 830Gly Pro Asp Ala Ser Leu Thr Gly Met Ala Arg Glu Ser Ala Ala Ala835 840 845Trp Ala Gly Asp Ala Pro Arg Pro Val Ala Val Ala Val Gln Arg Arg850 855 860Gly Arg Pro Glu Ala Gln Ser Phe Val Ser Ala Met Ala Gln Ala His865 870 875 880Val Arg Gly Val Gly Val Asp Trp Ala Ala Ala Phe Ala Gly His Glu885 890 895Thr Arg Arg Ala Pro Leu Pro Thr Tyr Ala Phe Gln Arg Asp Arg His900 905 910Trp Pro Asp Gly Leu Asp Glu Arg Gly Ala Arg Arg Pro Ser Thr Ser915 920 925Pro Val Val Pro Ser Thr Asp Arg Glu Pro Val Val Val Asp Ala Ser930 935 940Pro Ala Asp Arg Ala Ala Ser Pro Gly Glu Leu Leu Ala Leu Val Arg945 950 955 960Thr His Ala Ala Leu Val Leu Gly His Asn Ser Pro Asp Gly Ile Asp
965 970 975Pro Ala Leu Thr Phe Lys Gln Leu Gly Phe Asp Ser Leu Ala Ala Thr980 985 990Glu Leu Ser Glu Arg Leu Ser Ala Ala Thr Asp Thr Glu Leu Pro Ala995 1000 1005Thr Leu Thr Phe Asp His Pro Thr Pro Asn Ala Val Ala Ala Trp1010 1015 1020Leu Arg Ala Ala His Glu Gly Gln Pro Thr Ala Ala Pro Thr Ala1025 1030 1035Ala Thr Gly Pro Ser Met Ala Glu Asp Pro Val Ala Val Val Ala1040 1045 1050Val Ser Cys Arg Tyr Pro Gly Gly Val Glu Ser Gly Glu Ala Leu1055 1060 1065Trp Arg Leu Val Asp Glu Gly Val Asp Ala Val Gly Glu Phe Pro1070 1075 1080Gly Asp Arg Gly Trp Asp Leu Ala Glu Leu Phe Gly Arg Ala Pro1085 1090 1095Asp Gly Ser Gly Gly Ser Ala Thr Gly Arg Gly Gly Phe Leu Tyr1100 1105 1110Gly Ala Gly Asp Phe Asp Ala Glu Phe Phe Gly Ile Ser Pro Arg1115 1120 1125Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Ile Leu Leu Glu Leu1130 1135 1140Ser Trp Glu Leu Leu Glu Arg Ala Gly Ile Pro Pro Ala Ser Leu1145 1150 1155Ala Gly Ser Ala Thr Gly Val Tyr Val Gly Ala Thr Ala Val Asp1160 1165 1170Tyr Gly Pro Arg Leu His Glu Ala Thr Ala Glu Leu Asp Gly His1175 1180 1185Leu Leu Thr Gly Ser Thr Pro Ser Val Ala Ser Gly Arg Val Ala1190 1195 1200Tyr Ala Leu Gly Leu Glu Gly Pro Ala Leu Thr Val Asp Thr Ala1205 1210 1215Cys Ser Ser Ser Leu Val Ala Met His Leu Ala Ala Gln Ala Leu1220 1225 1230Arg Gln Gly Glu Cys Asp Leu Ala Leu Ala Gly Gly Val Thr Val1235 1240 1245Met Ala Thr Pro Gly Met Phe Thr Ser Phe Ser Arg Gln Arg Gly1250 1255 1260Leu Ala Pro Asp Gly Arg Cys Lys Pro Phe Ala Ala Ala Ala Asp1265 1270 1275Gly Thr Gly Trp Ser Glu Gly Ala Gly Leu Val Leu Leu Glu Arg1280 1285 1290Leu Ser Asp Ala Arg Arg Asn Gly His Gln Val Leu Ala Val Ile1295 1300 1305Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Ser1310 1315 1320Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu1325 1330 1335Ala Asn Ala Arg Leu Glu Pro Ala Asp Val Asp Ala Val Glu Ala1340 1345 1350His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln Ala1355 1360 1365Leu Leu Ala Thr Tyr Gly Gly Gln Arg Thr Asp Asp Arg Pro Leu1370 1375 1380Trp Leu Gly Ser Ile Lys Ser Asn Ile Gly His Thr Gln Ala Ala1385 1390 1395Ala Gly Val Ala Gly Val Ile Lys Met Val Met Ala Leu Arg His1400 1405 1410Gly Arg Leu Pro Ala Ser Leu His Ile Asp Ala Pro Ser Pro His1415 1420 1425Ile Asp Trp Ser Asp Gly Thr Val Arg Leu Leu Ser Glu Pro Val1430 1435 1440Asp Trp Pro Gly Thr Asp Trp Pro Gly Ser Asp Arg Pro Arg Arg1445 1450 1455Ala Ala Val Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His Leu1460 1465 1470Ile Leu Glu Gln Ala Pro Asp His Pro Glu Pro Glu Pro Thr Thr1475 1480 1485Ser Gly Gly Val Val Pro Trp Val Leu Ser Ala Arg Thr Ala Asp1490 1495 1500Ala Leu Arg Ala Gln Ala Gly Arg Leu Ala Glu Trp Val Thr Ala1505 1510 1515Gly Ala Pro Arg Ser Pro Ala Ser Pro Thr Ser Pro Ala Ser Pro1520 1525 1530Ala Asp Val Gly Trp Ser Leu Ala Thr Thr Arg Ser Ala Asp Arg1535 1540 1545His Arg Ala Val Val Ser Gly Thr Asp Arg Asp Glu Leu Leu Ser1550 1555 1560Gly Leu Arg Ala Val Ala Asp Gly Leu Ala Pro Ala Ala Val Ser1565 1570 1575Ala Gly Ala Ala Pro Gly Pro Val Met Val Phe Pro Gly Gln Gly1580 1585 1590Ser Gln Trp Arg Gly Met Gly Val Glu Leu Leu Asp Ser Ser Pro1595 1600 1605Val Phe Ala Ala Arg Met Ala Ala Cys Glu Ala Ala Leu Gly Glu
1610 1615 1620Phe Val Asp Trp Ser Leu Thr Ala Val Leu Arg Gly Ala Pro Gly1625 1630 1635Ala Pro Glu Pro Ser Arg Val Asp Val Leu Gln Pro Cys Leu Trp1640 1645 1650Ala Val Met Val Ser Leu Ala Ala Val Trp Glu Ser Tyr Gly Val1655 1660 1665Thr Pro Thr Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala1670 1675 1680Ala Cys Val Ala Gly Gly Leu Ser Leu Arg Asp Gly Ala Arg Val1685 1690 1695Val Ala Leu Arg Ser Gln Ala Leu Arg Ala Leu Ala Gly His Gly1700 1705 1710Thr Met Ala Ser Leu Ala Leu Ser Gly Ala Glu Ala Glu Arg Phe1715 1720 1725Leu Ala Asp Leu Gly Ala Ala Ala Ala Arg Val Thr Val Ala Val1730 1735 1740Phe Asn Gly Pro Tyr Ser Thr Val Val Ser Gly Pro Thr Asp Gln1745 1750 1755Val Ala Ala Val Val Ala Ala Cys Glu Ala Ala Gly His Arg Ala1760 1765 1770Arg Thr Ile Asp Val Asp Tyr Ala Ser His Gly Pro Gln Val Asp1775 1780 1785Arg Leu Ala Asp Thr Ile Arg Thr Asp Leu Ala Asp Leu Ser Pro1790 1795 1800Gly Ala Ser Asp Ala Val Phe Tyr Ser Ala Val Thr Gly Ala Arg1805 1810 1815Gln Pro Thr Glu Glu Leu Asp Ala Asp Tyr Trp Phe Thr Asn Leu1820 1825 1830Arg Gln Pro Val Arg Phe Ala Ser Ala Ile Asp Ala Leu Leu Ala1835 1840 1845Ala Gly Tyr Arg Val Phe Ile Glu Val Ser Pro His Pro Val Leu1850 1855 1860Ile Pro Ala Leu Arg Glu Cys Phe Glu Glu Ala Glu Val Ala Ala1865 1870 1875Ala Thr Val Pro Thr Leu Arg Arg Asp Gln Gly Gly Pro Asp Gln1880 1885 1890Val Ala Arg Ala Leu Gly Asp Gly Phe Val Ala Gly Leu Ala Val1895 1900 1905Asp Trp Ser Arg Trp Phe Val Gly Asp Gly Arg Glu Ala Gly Asp1910 1915 1920Glu Gly His Arg Pro Arg Thr Val Glu Leu Pro Thr Tyr Pro Phe1925 1930 1935Gln Arg Arg Arg Tyr Trp Leu Ala Pro Asp His Gly Arg Arg Glu1940 1945 1950Gly Arg Thr Ala Gly Val Gly Thr Arg Pro Ala Gly His Ala Leu1955 1960 1965Leu Ser Ser Ala Val Glu Leu Ala Asp Gly Gly Leu Val Leu Ser1970 1975 1980Gly Arg Leu Pro Gly Asp Ala Ala Trp Val Gly Ala His Thr Val1985 1990 1995Ala Gly Val Gln Leu Val Pro Gly Ala Val Leu Val Asp Trp Ala2000 2005 2010Leu Leu Ala Ala Asp Glu Ala Gly Gly Ala Ser Leu Glu Glu Leu2015 2020 2025Leu Leu Arg Ala Pro Leu Glu Leu Ser Gly Pro Ser Gly Leu Ser2030 2035 2040Glu Pro Ser Ala Gly Val Leu Ala Gln Val Ala Val Gly Ala Pro2045 2050 2055Asp Glu Ser Gly Arg Arg Glu Leu Arg Ile Ser Ser Arg Pro Ala2060 2065 2070Asp Ala Gly Ala Gly Glu Gly Trp Thr Cys His Ala Val Gly Ser2075 2080 2085Leu Ala Pro Gly Gly Pro Pro Ala Pro Ala Asp Thr Gly Thr Ala2090 2095 2100Thr Val Pro Trp Pro Pro Ala Gly Ala Glu Ala Leu Asp Pro Ala2105 2110 2115Gly Leu Tyr Glu Arg Ala Glu Arg Arg Gly Tyr Gly Tyr Gly Pro2120 2125 2l30Ala Leu Arg Gly Val Val Ala Leu Trp Arg Asp Gly Ala Asp Leu2135 2140 2145Val Ala Asp Val Ala Leu Pro Glu Glu Ala Gly Gly Gly Gly Glu2150 2155 2160Gly Gly Ala Asp Gly Asp Gly Thr Ala Gly Phe Gly Leu His Pro2165 2170 2175Val Leu Leu Asp Ala Ala Leu Gln Pro Ala Leu Leu Ala Glu Pro2180 2185 2190Asp Gly Thr Gly Gly Glu Gly Ala Gly Pro Glu Ala Arg Leu Trp2195 2200 2205Leu Pro Phe Ala Trp Ser Gly Val Arg Leu Trp Ala Thr Gly Ala2210 2215 2220Arg Ala Ala Arg Val Arg Leu Ser Pro Leu Asp Gly Gly Gly Gly2225 2230 2235Asp Val Ala Asp Glu Arg Glu Leu Arg Ile Glu Val Ser Asp Pro2240 2245 2250Thr Gly Ala Pro Val Leu Ser Val Ala Ser Val Val Leu Arg Pro
2255 2260 2265Arg Thr Val Arg Gln Val Arg Glu Ala Ser Gly Ala Ala Ala Gly2270 2275 2280Gly Leu Phe Ala Leu Asp Trp Thr Pro Val Ala Pro Gln Glu Pro2285 2290 2295Ser Gly Ala Glu Asp Asp Ala Gly Cys Val Ala Val Leu Gly Glu2300 2305 2310Ala Pro Thr Glu Pro Gly Val Asp Gly Cys Arg Asp Thr Tyr Thr2315 2320 2325Asp Leu Pro Ala Leu Leu Ala Ala Leu Asp Ala Gly Ala Pro Leu2330 2335 2340Pro Ser Val Val Met Trp Arg Pro Pro Ala Ala Asp Pro Gly Ala2345 2350 2355Ala Pro Glu Asp Ala Ala Leu Ser Ala Val Arg Gly Val Ala Ala2360 2365 2370Ala Leu Arg Ala Trp Val Ala Glu Pro Arg Leu Thr Val Ser Arg2375 2380 2385Leu Ala Val Val Thr Arg Gly Ala Val Ala Ala Gly Gly Ala Glu2390 2395 2400Gly Glu Pro Val Asp Leu Ala Ala Ala Ala Ala Trp Gly Cys Ala2405 2410 2415Arg Gly Val Gln Ala Glu His Pro Asp Arg Ile Val Leu Val Asp2420 2425 2430Val Asp Asp Asp Val Asp Met Gly Ala Asp Thr Asp Thr Asp Ile2435 2440 2445Gly Ala Ala Ala Gly Leu Ala Ala Ala Leu Gly Glu Pro Gln Val2450 2455 2460Ala Leu Arg Gly Asp Thr Leu Leu Ala Pro Arg Leu Ala Arg Ser2465 2470 2475Ala Ala Thr Pro Gly Gly Val Ala Phe Asp Pro Asn Gly Thr Val2480 2485 2490Leu Val Thr Asp Ser Gly Gly Pro Leu Ala Gly Ser Val Ala Glu2495 2500 2505His Leu Val Arg Ala Glu Gly Val Arg His Leu Leu Leu Val Arg2510 2515 2520Phe Glu Gly Ala Asp Gly Ala Tyr Asp Thr Tyr Asp Arg Gln Asp2525 2530 2535Ala Gln Val His Met Val Thr Val Asp Pro Arg Asp Thr Ala Ala2540 2545 2550Leu Glu Arg Val Val Ala Gln Val Asp Pro Ala His Pro Leu Thr2555 2560 2565Gly Val Val His Val Ala Gly Leu Ser Ala Asp Ile Glu Thr Ser2570 2575 2580Gly Ala Ala Arg Gly Trp Ala Val Ala Ala Gly Val Val Arg Ala2585 2590 2595Leu His Gln Ala Thr Ala Ala Leu Pro Ser Val Arg Phe Val Thr2600 2605 2610Leu Ser Asp Ala Ala Thr Ala Trp Asp Gly Pro Ala Ala Pro Glu2615 2620 2625Arg Ala Ala Ala Gly Ala Phe Cys Ala Ala Val Thr Asp Val Arg2630 2635 2640Arg Arg Ala Gly Leu His Gly Leu Asp Val Ala Phe Gly Pro Trp2645 2650 2655Ala Ala Ala Asp Asp Asp Gly Gly Ala Asp Ser Gly Gly Arg Trp2660 2665 2670Thr Gly Val Leu Gly Ala Asp Arg Gly Leu Ala Leu Leu Arg Ala2675 2680 2685Ala Cys Arg Ala Asp Arg Pro Arg Leu Val Ala Ala Asp Ile Arg2690 2695 2700Thr Arg Ala Leu Thr Ala His Pro Ala His Glu Leu Pro Ala Ala2705 2710 2715Leu Arg Thr Leu Gly Ala Ser Ala Ser Ala Ser Ala Gly Gly Arg2720 2725 2730Ala Pro Val Arg Arg Val Ala Ala Ala Ala Pro Gly Arg Thr Thr2735 2740 2745Asp Trp Ala Ser Arg Leu Val Gly Leu Gly Pro Ala Glu Arg Arg2750 2755 2760Arg Ala Val Leu Glu Leu Val Arg Asp His Ala Ala Ala Val Leu2765 2770 2775Gly Gln Pro Asp Pro Lys Ala Val Arg Ala Asp Ala Ser Phe Lys2780 2785 2790Glu Leu Gly Phe Asp Ser Val Thr Ala Val Glu Leu Arg Asp Arg2795 2800 2805Leu Val Ala Val Gly Gly Leu Arg Leu Pro Ala Ala Val Val Phe2810 2815 2820Arg His Pro Thr Pro Glu Ala Leu Ala His Arg Ile Glu Gln Gln2825 2830 2835Leu Ala Pro Asp Asp Thr Asn Asn Ala Ala Ile Thr Asp Asn Ala2840 2845 2850Asp Asn Ala Ala Lys Ser Asn Gly Asn Ser Asn Gly Thr Ala Leu2855 2860 2865Asp Ala Ala Asp Lys Leu Ala Ser Ala Thr Ala Asp Glu Ile Leu2870 2875 2880Asp Phe Ile Asp Asn Glu Leu Gly Val Leu Ser Glu Ala Arg Pro2885 2890 2895Arg Pro Ser Asn
2900<210>10<211>2223<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met Val Ser Glu Glu Lys Leu Val Glu Tyr Leu Arg Arg Val Thr Thr1 5 10 15Glu Leu His Asp Ala Arg Thr Arg Leu Arg Glu Leu Glu Glu Gly Glu20 25 30Gln Glu Pro Val Ala Val Val Gly Met Ala Cys Arg Phe Pro Gly Gly35 40 45Val Arg Ser Pro Glu Asp Leu Arg Arg Leu Val Leu Ser Gly Gly Asp50 55 60Ala Ile Gly Asp Phe Pro Thr Asp Arg Gly Trp Asp Leu Asp Gly Leu65 70 75 80Phe His Pro Asp Pro Ala His Phe Gly Thr Ser Tyr Val Ser Gln Gly85 90 95Gly Phe Leu Tyr Asp Val Asp Arg Phe Asp Ala Gly Phe Phe Gly Ile100 105 110Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu115 120 125Glu Leu Ser Trp Glu Ala Leu Glu Ser Ala Gly Val Val Pro Gly Ala
130 135 140Leu Arg Ala Ser Arg Thr Gly Val Tyr Val Gly Val Ser Ser Glu Asp145 150 155 160Tyr Ile Ser Gly Leu Pro Gln Ile Pro Glu Gly Phe Glu Gly Tyr Ala165 170 175Thr Thr Gly Ser Leu Thr Ser Val Ile Ser Gly Arg Val Ala Tyr Thr180 185 190Phe Gly Phe Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser195 200 205Ser Met Val Ala Ile His Leu Ala Gly Gln Ala Leu Arg Gln Gly Glu210 215 220Cys Ser Leu Ala Leu Ala Gly Gly Val Thr Val Leu Ser Thr Pro Leu225 230 235 240Met Phe Thr Glu Phe Cys Arg Gln Arg Ala Leu Thr Pro Asp Ala Arg245 250 255Cys Lys Pro Phe Ala Ala Ala Ala Asp Gly Thr Gly Phe Ser Glu Gly260 265 270Ala Gly Leu Leu Leu Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly275 280 285His Glu Val Leu Ala Val Leu Arg Gly Ser Ala Ile Asn Gln Asp Gly290 295 300Ala Ser Asn Gly Leu Thr Ala Pro Asn Asp Val Ala Gln Glu Ser Val305 310 315 320Ile Arg Asp Ala Leu Ala Arg Ala Gly Leu Ser Gly Ala Asp Val Asp325 330 335Met Val Glu Ala His Gly Thr Gly Thr Arg Leu Gly Asp Pro Ile Glu340 345 350Ala Glu Ala Leu Ile Ala Thr Tyr Gly Ala Asp Arg Pro Ala Asp Arg355 360 365Pro Leu Tyr Leu Gly Ser Ile Lys Ser Asn Ile Gly His Thr His Ala370 375 380Ala Ala Gly Val Ala Gly Ala Ile Asn Thr Val Met Ala Leu Arg Asp385 390 395 400Gly Lys Leu Ala Arg Thr Leu His Ile Asp Glu Pro Thr Arg His Val405 410 415Asp Trp Ser Ala Gly Thr Val Arg Leu Leu Thr Asp Pro Tyr Asp Trp420 425 430Pro Val Ala Asp Arg Pro Arg Arg Ala Ala Val Ser Ser Phe Gly Val435 440 445Ser Gly Thr Asn Ala His Val Ile Leu Glu Gln Ala Pro Asp Ala Gly450 455 460Ala Gln Gln Asp Ala Arg Gln Arg Gly Gly Asp Thr Phe His Gly Val465 470 475 480Val Pro Trp Pro Val Ser Gly Arg Thr Glu Ala Ala Leu Arg Asp Gln485 490 495Ala Ala Arg Leu Gly Ala Phe Leu Thr Ala Asp Gly Ala Thr Ala Asn500 505 510Gly Ala Ala Thr Gly Gly Val Ala Asp Val Gly Trp Ser Leu Ala Met515 520 525Arg Arg Thr Ala Phe Glu His Arg Ala Val Val Val Gly Arg Asp Arg530 535 540Ser Asp Leu Leu Ala Ala Leu Glu Gly Leu Ala Ala Asp Glu Pro Gly545 550 555 560Pro Ala Val Val Arg Gly Val Ala Ala Asp Val Gly Ala Gly Pro Val565 570 575Met Val Phe Pro Gly Gln Gly Ser Gln Trp Leu Gly Met Gly Val Glu580 585 590Leu Leu Asp Ser Ser Pro Val Phe Ala Ala Arg Ile Ala Ala Cys Glu595 600 605Arg Ala Leu Ala Ala His Val Asp Trp Ser Leu Thr Asp Val Leu Arg610 615 620Gly Ala Arg Gly Ala Ala Asp Ile Gly Arg Val Asp Val Val Gln Pro625 630 635 640Val Leu Trp Ala Val Met Val Ser Leu Ala Ala Val Trp Glu Ala His645 650 655Gly Val Arg Pro Ser Ala Val Val Gly His Ser Gln Gly Glu Ile Ala660 665 670Ala Ala Cys Val Ala Gly Ala Met Thr Leu Glu Asp Gly Ala Arg Val675 680 685Val Ala Leu Arg Ala Arg Ala Leu Arg Ala Leu Ala Gly Tyr Gly Ala690 695 700Met Ala Ser Leu Gly Cys Gly Val Glu Glu Thr Glu Arg Leu Thr Ala705 710 715 720Val His Ala Pro Asp Val Ala Val Ala Ala Val Asn Gly Pro Ser Ser725 730 735Thr Val Val Ser Gly Pro Ser Glu Gln Val Glu Lys Leu Val Ala Ala740 745 750Val Arg Ala Asp Gly Leu Arg Ala Arg Ala Ile Asp Val Asp Tyr Ala755 760 765Ser His Gly Pro Gln Val Asp Arg Ile Ala Asp Glu Leu Ala Asp Val770 775 780Leu Ala Gly Val Ser Gly Ala Ala Thr Asp Thr Ala Phe Tyr Ser Thr785 790 795 800Val Thr Gly Ala Arg Met Asp Ala Ser Gly Leu Asp Ala Gly Tyr Trp805 810 815Phe Thr Asn Leu Arg Gln Pro Val Arg Phe Ala Glu Ala Val Gln Ala
820 825 830Leu Leu Asp Ala Asp Tyr Arg Val Phe Ile Glu Val Ser Ala His Pro835 840 845Val Leu Leu Leu Gly Leu Gln Glu Cys Phe Glu Ala Ala Gly Arg Pro850 855 860Ala Val Ala Ile Gly Thr Leu Arg Arg Asp Glu Gly Gly Pro Glu Arg865 870 875 880Leu Cys Arg Ala Leu Ala Glu Ala His Val Ala Gly Val Ala Val Asp885 890 895Trp Ala Ser Trp Tyr Ala Asp Gly Pro Ala Pro Ala Ala Val Pro Leu900 905 910Pro Ala Tyr Ala Phe Gln Arg Glu Arg Tyr Trp Leu Pro Ala Gly Ala915 920 925Gly Ser Gly Pro Gly Asp Val Ala Gly Ala Gly Leu Thr Ala Val Gly930 935 940His Ala Leu Leu Pro Val Ser Val Arg Leu Ala Asp Gly Ser Leu Val945 950 955 960Leu Thr Gly Arg Leu Pro Glu Ala Ala Arg Ala Gly Trp Leu Ala Glu965 970 975His Leu Val Ala Asp Leu Pro Leu Leu Pro Gly Thr Val Leu Val Glu980 985 990Trp Val Leu Arg Ala Ala Asp Glu Ala Gly Cys Gly Gly Val Glu Glu995 1000 1005Leu Ala Leu Gln Val Pro Val Ala Leu Pro Val Ser Gly Gly Leu1010 1015 1020Val Ile Gln Val Val Val Asp Ala Ala Glu Gly Asp Gly Arg Arg1025 1030 1035Pro Val Arg Val His Ser Arg Pro Glu Glu Asp Ser Gly Ala Pro1040 1045 1050Asp Ala Trp Val Cys His Val Ser Gly Thr Leu Leu Pro Gly Val1055 1060 1065Ala Gly Pro Val Pro Pro Ser Gly Pro Gly Gly Ala Trp Pro Pro1070 1075 1080Pro Gly Ala Arg Pro Ala Ala Ile Asp Gly Phe Tyr Glu Arg Ala1085 1090 1095Glu Ala Ala Gly Tyr Gly Tyr Gly Ala Phe Phe Arg Gly Leu Thr1100 1105 1110Asn Val Trp His Asp Gly Glu Asp Thr Leu Ala Glu Val Val Leu1115 1120 1125Pro Lys Glu Ala Ala Glu Gln Ala Gly Gly Phe Gly Ile His Pro1130 1135 1140Ala Leu Leu Asp Ala Ala Met Gln Pro Val Leu Leu Ala Gly Gln1145 1150 1155Leu Arg Gln Cys Ala Ala Ala Ala Gly Ala Asp Thr Ala Ser Gly1160 1165 1170Thr Val Leu Leu Pro Phe Thr Trp Ser Gly Val Arg Leu Trp Ala1175 1180 1185Gly Gly Ala Thr Arg Leu Arg Val Arg Leu Ser Pro Arg Pro Glu1190 1195 1200Gly Leu Arg Val Leu Leu Ala Asp Ala Thr Gly Ala Pro Val Leu1205 1210 1215Thr Ala Asp Ala Val Ala Leu Arg Glu Thr Gly Val Gln Gln Leu1220 1225 1230Arg Ala Ser Ser Arg Val Arg Gly Ser His Gly Leu Phe Ala Val1235 1240 1245Glu Trp Val Pro Pro Leu Ser Ala Thr Ala Gly Gly Thr Ala Pro1250 1255 1260Ala Thr Leu Ala Val Leu Gly Asp Asp Ala Pro Asp Leu Ala Asp1265 1270 1275Ala Asp Arg Tyr Pro Asp Leu Asp Ala Leu Phe Arg Ala Val Ala1280 1285 1290Asp Gly Ala Pro Ala Pro Asp Val Val Ile Ala Ser Val Arg Thr1295 1300 1305Gly Asn Asp Pro Ala Gly Ser Asp Thr Gly Leu Ala Thr Ala Arg1310 1315 1320Arg Thr Leu Thr Leu Ala Gln Glu Trp Leu Ala Gly Ser Gly Ala1325 1330 1335Asp Gly Ala Arg Leu Ala Val Val Thr Arg Ser Ala Ile Arg Thr1340 1345 1350Gly Asp Asp Gly Gln Glu Arg Val Val Pro Ser Ala Ala Ala Val1355 1360 1365Trp Gly Leu Met Arg Ser Ala Gln Thr Glu His Pro Gly Arg Phe1370 1375 1380Val Leu Ile Asp Glu Asp Thr Asp Ser Thr Glu Asn Ile Leu Glu1385 1390 1395Ala Val Arg Thr Asp Glu Pro Gln Leu Ala Leu Arg Gly Gly Arg1400 1405 1410Ala Leu Val Pro Arg Met Ala Arg Val Asp Ala Glu Pro Glu Leu1415 1420 1425Thr Ala Pro Ser Gly Glu Arg Ala Trp His Val Ala Ala Gly Lys1430 1435 1440Thr Gly Pro Asp Asp Leu Thr Ala Val Pro Ser Pro Arg Ala Ser1445 1450 1455Ala Pro Leu Ala Pro Gly Gln Val Arg Ile Ala Val Arg Ala Ala1460 1465 1470Gly Leu Asn Phe Arg Asp Ala Leu Ile Ala Leu Asp Met Tyr Pro
1475 1480 1485Asp Ala Ser Ala Ser Ile Gly Ser Glu Gly Ala Gly Val Val Leu1490 1495 1500Glu Val Ser Glu Gly Val Ala Gly Val Ala Val Gly Asp Arg Val1505 1510 1515Met Gly Leu Phe Asn Asp Ala Phe Gly Pro Val Ala Val Ala Asp1520 1525 1530Ala Arg Met Val Ala Pro Val Pro Asp Gly Trp Ser Phe Arg Glu1535 1540 1545Ala Ala Ala Ala Pro Val Ala Phe Leu Thr Ala Trp Tyr Gly Leu1550 1555 1560Val Asp Leu Gly Gly Leu Ser Ser Gly Glu Thr Val Val Ile His1565 1570 1575Gly Ala Ala Gly Gly Val Gly Met Ala Ala Val Gln Val Ala Arg1580 1585 1590His Leu Gly Ala Glu Val Phe Ala Thr Ala Ser Pro Ala Lys His1595 1600 1605Pro Val Leu Glu Gly Met Gly Val Asp Ala Ala His Arg Ala Ser1610 1615 1620Ser Arg Asp Leu Gly Phe Glu Ala Ala Phe Ser Ser Ala Thr Gly1625 1630 1635Gly Arg Gly Val Asp Val Val Leu Asn Ser Leu Ala Gly Glu Phe1640 1645 1650Thr Asp Ala Ser Leu Arg Leu Leu Ala Pro Gly Gly Arg Leu Ile1655 1660 1665Glu Met Gly Lys Thr Asp Val Arg Asp Pro Asp Gln Val Ala Arg1670 1675 1680Glu His Ser Val Ala Tyr Arg Ala Phe Asp Leu Ile Ala Asp Ala1685 1690 1695Gly Pro Glu Arg Ile Gly Gln Leu Leu Ala Ala Leu Gly Glu Arg1700 1705 1710Phe Ala Asp Gly Ala Phe Thr Pro Leu Pro Val Thr Gly Trp Arg1715 1720 1725Leu Gly Gln Ala Arg Gln Ala Leu Arg Gln Leu Ser Gln Ala Arg1730 1735 1740His Thr Gly Lys Leu Val Leu Asp Val Asp Pro Ala Pro Asp Pro1745 1750 1755Asp Gly Thr Val Leu Ile Thr Gly Gly Thr Gly Thr Leu Gly Gly1760 1765 1770Leu Ile Ala Glu His Leu Val Arg Ser Arg Gly Val Arg His Leu1775 1780 1785Leu Leu Leu Ser Arg Arg Gly Pro Asp Ala Pro Gly Ala Glu Glu1790 1795 1800Leu Thr Ala Arg Leu Thr Glu Leu Gly Ala Arg Val Arg Val Ala1805 1810 1815Ala Val Asp Val Gly Asp Ala Thr Ala Leu Gly Glu Ala Val Ala1820 1825 1830Gly Val Asp Pro Ala His Pro Leu Thr Gly Val Val His Ala Ala1835 1840 1845Gly Val Val Ala Asp Ala Met Leu Pro Ser Gln Asp Asp Glu Arg1850 1855 1860Leu Val Ala Ala Trp Ser Ala Lys Ala Ala Ala Ala Ala Arg Leu1865 1870 1875His Asp Ala Thr Ala Gly Leu Pro Leu Gly Met Phe Val Leu Phe1880 1885 1890Ser Ser Phe Ala Ser Thr Leu Gly Thr Ala Gly Gln Ala Asn Tyr1895 1900 1905Ala Ala Ala Asn Ala Tyr Cys Asp Ala Leu Val Glu Arg Arg His1910 1915 1920Ala Glu Gly Leu Pro Gly Val Ser Val Ser Trp Gly Leu Trp Ser1925 1930 1935Ala Ala Ser Gly Leu Thr Gly Gly Leu Thr Glu Ala Asp Val Ala1940 1945 1950Arg Ile Ala Arg Gln Gly Ile Val Pro Asn Ser Thr Glu Gln Gly1955 1960 1965Tyr Asp Leu Phe Asp Ala Ala Leu Gly His Gly Arg Pro Ala Leu1970 1975 1980Leu Ala Leu Asn Leu Asp Thr Arg Ala Leu Ala Ala Gln Pro Val1985 1990 1995Ala Ala Leu Pro Ala Pro Leu Arg Ala Leu Ala Ala Asp Ala Gln2000 2005 2010Ala Ala Gly Ala Arg Ser Gly Gly Ala Ala Ala Arg Pro Thr Ala2015 2020 2025Ala Ala Ala Glu Glu Pro Ala Asp Trp Ala Ala Arg Leu Arg Ala2030 2035 2040Leu Ala Pro Ala Glu Gln Arg Arg Leu Leu Thr Asp Leu Val Arg2045 2050 2055Arg His Ala Ala Thr Val Leu Gly His Ala Asp Pro Glu Ala Val2060 2065 2070Pro Ala Asp Ala Ala Phe Lys Glu Leu Gly Phe Asp Ser Leu Thr2075 2080 2085Ala Val Glu Leu Arg Asn Arg Val Thr Ala Ala Thr Gly Leu Arg2090 2095 2100Leu Pro Ala Thr Val Ile Phe Asp Tyr Pro Glu Pro Gly Ala Leu2105 2110 2115Ala Glu Arg Leu Arg Thr Glu Leu Ala Pro Glu Glu Gly Ala Ser
2120 2125 2130Ala Thr Ala Pro Asp Leu Tyr Ala Pro Val Leu Ser Arg Leu Thr2135 2140 2145Gly Leu Glu Glu Thr Leu Ala Ala Leu Ala Ser Ser Gly Val Asn2150 2155 2160Gly Gly Val Asn Gly Gly Val Ala Asp Pro Gly Ala Val Thr Ala2165 2170 2175Arg Leu Glu Ser Leu Leu Ala Asp Trp Lys Ala Ala His Ala Pro2180 2185 2190Ser Arg Asn Gly Gly Thr Ala Ala Glu Arg Leu Glu Ala Ala Thr2195 2200 2205Thr Asp Gln Val Leu Asp Phe Ile Asp Lys Glu Leu Gly Val Gln2210 2215 2220<210>11<211>4032<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met Ser Gly Gly Ala Val Thr Thr Glu Thr Asn Glu Glu Arg Leu Val1 5 10 15Asp Tyr Leu Lys Arg Val Ala Ala Asp Leu His Asp Thr Arg Ala Arg20 25 30Leu Arg Glu Val Glu Asp Gly Gln Arg Glu Pro Val Ala Ile Val Ala
35 40 45Met Ala Cys Arg Tyr Pro Gly Asp Val Ala Ser Pro Glu Asp Leu Trp50 55 60Asp Leu Val Ala Ala Arg Arg His Ala Met Thr Ala Phe Pro Asp Asn65 70 75 80Arg Gly Trp Asp Leu Glu Arg Leu Phe His Pro Asp Pro Asp His Pro85 90 95Gly Thr Ser Tyr Ala Arg Glu Gly Gly Phe Leu His Asp Ala Asp Leu100 105 110Phe Asp Pro Glu Phe Phe Gly Ile Ser Pro Arg Glu Ala Ala Ala Val115 120 125Asp Pro Gln Gln Arg Leu Leu Leu Glu Val Ala Trp Glu Ala Leu Glu130 135 140Arg Ala Gly Ile Ala Pro Gly Ser Leu Lys Gly Ala Pro Val Gly Val145 150 155 160Tyr Ala Gly Thr Ala Leu Pro Gly Phe Gly Thr Pro His Ile Asp Arg165 170 175Ala Ala Glu Gly Tyr Leu Val Thr Gly Asn Ala Pro Ser Val Leu Ser180 185 190Gly Arg Val Ala Tyr Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Val195 200 205Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Met His Leu Ala Ala Gln210 215 220Ala Leu Arg Gln Gly Glu Cys Glu Leu Ala Leu Ala Gly Gly Val Thr225 230 235 240Val Met Thr Thr Pro Tyr Val Phe Thr Glu Phe Ala Arg Gln Arg Gly245 250 255Leu Ala Ala Asp Ser Arg Cys Lys Ala Phe Ser Lys Gly Ala Asp Gly260 265 270Thr Ala Phe Ala Glu Gly Ala Gly Leu Leu Val Leu Glu Arg Leu Ser275 280 285Asp Ala Gln Arg Asn Gly His Gln Val Leu Ala Val Met Arg Gly Ser290 295 300Ala Ile Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly305 310 315 320Leu Ala Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Gly Ala Arg Leu325 330 335Ser Pro Ala Asp Val Asp Val Val Glu Ala His Gly Thr Gly Thr Thr340 345 350Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln355 360 365Glu Arg Pro Glu Gly Arg Pro Leu Trp Leu Gly Ala Ile Lys Pro Asn370 375 380Leu Gly His Thr Gln Gly Ala Ala Gly Val Ala Gly Val Ile Lys Met385 390 395 400Val Met Ala Leu Arg Asn Ala Ser Leu Pro Ala Leu Leu His Ala Asp405 410 415Arg Pro Thr Ser Val Val Asp Trp Asp Gly Gly Ala Val Arg Leu Leu420 425 430Ala Glu Pro Val Ala Trp Pro Ala Gly Asp Arg Arg Arg Arg Ala Gly435 440 445Val Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His Leu Ile Leu Glu450 455 460Glu Ala Pro Pro Arg Pro Asp Thr Glu Leu Pro Ala Ser Val Pro Gln465 470 475 480Arg Pro Glu Gly Thr Val Val Pro Trp Val Val Ser Ala Arg Gly Ala485 490 495Val Ser Leu Arg Thr Gln Ala Ala Ala Leu Ala Glu His Met Ala Ala500 505 510His Pro Asp Thr Pro Val Asp Ala Ile Gly Trp Ser Leu Ala Thr Thr515 520 525Arg Ser Pro Leu Asp His Arg Ala Val Val Leu Gly Ala Asp Arg Gly530 535 540Glu Leu Ser Ala Arg Leu Ala Asp Leu Ala Glu Gly Arg Thr His Pro545 550 555 560Asp Val Thr Arg Ala Ser Ala Pro Ala Arg Leu Gly Gly Ser Ala Phe565 570 575Leu Phe Thr Gly Gln Gly Ser Gln Arg Pro Gly Met Gly Ala Gln Leu580 585 590Tyr Arg Ala Tyr Pro Ala Phe Ala Ala Ala Phe Asp Glu Ala Cys Ala595 600 605Ala Leu Asp Pro His Leu Gly Arg Ser Leu Leu Glu Leu Val Phe Ala610 615 620Pro Ala Asp Thr Asp Gly Asp Gly Asp Ala Asp Arg Ala Ser Ala Leu625 630 635 640Asp Ala Thr Glu Val Thr Gln Ala Ala Leu Phe Ala Val Glu Val Ala645 650 655Leu Tyr Arg Leu Val Glu Ser Phe Gly Val Val Pro Gly Tyr Leu Ala660 665 670Gly His Ser Val Gly Glu Leu Val Ala Ala His Ile Ala Gly Val Leu675 680 685Ser Leu Pro Asp Ala Ala Arg Leu Val Ala Ala Arg Gly Arg Leu Met690 695 700Gln Ala Leu Pro Glu Gly Gly Ala Met Val Ala Leu Glu Ala Ala Glu705 710 715 720Glu Glu Val Ala Leu Leu Leu Ala Gly Arg Ala Asp Gln Val Ala Leu
725 730 735Ala Ala Val Asn Ala Pro Thr Ser Val Val Val Ser Gly Asp Glu Glu740 745 750Ala Val Glu Glu Ile Ala Arg Thr Ile Arg Glu Arg Gly His Arg Thr755 760 765Arg Arg Leu Arg Val Gly His Ala Phe His Ser Pro Arg Ile Asp Pro770 775 780Met Leu Glu Glu Phe Arg Gln Val Ala Ala Ser Leu Thr Tyr Ser Gln785 790 795 800Pro Arg Ile Ala Val Val Ser Asn Val Thr Gly Ala Leu Ala Gly Ala805 810 815Glu Gln Leu Cys Asp Pro Asp Tyr Trp Val Arg His Ala Arg Gln Pro820 825 830Val Arg Phe Arg Asp Gly Ile Ala Ala Leu Arg Ala Glu Gly Val Thr835 840 845Arg Phe Leu Glu Leu Gly Pro Asp Ala Val Leu Thr Ala Met Ala Arg850 855 860Asp Cys Leu Thr Ser Glu Ala Ala Pro Glu Val Ala Ala Gly Glu Ser865 870 875 880Ala Gln Asp Ala Gly Thr Ala Ser Gly Pro Ser Ala Pro Val Leu Ala885 890 895Thr Val Leu Arg Lys Gly Arg Asp Glu Pro Arg Thr Leu Leu Thr Ala900 905 910Leu Ala Gln Leu His Val Asp Gly Glu Ser Val Asp Phe Ser Ala Ser915 920 925Phe Pro Ala Thr Thr Gln Ala Thr Asp Leu Pro Thr Tyr Arg Phe Asp930 935 940Arg Arg Arg Tyr Trp Arg Asp Ala Pro Gln Ala Glu Ala Asp Val Arg945 950 955 960Ala Ala Gly Leu Glu Ala Ser Asp His Pro Leu Leu Arg Ala Ala Leu965 970 975Glu Pro Ala Asp Gly Gly Leu Leu Leu Thr Gly Arg Leu Ser Leu Arg980 985 990Gly Gln Pro Trp Leu Ala Asp His Ala Ile Val Asp Ala Val Pro Leu995 1000 1005Pro Gly Thr Leu Phe Val Glu Leu Ala Leu Gln Ala Gly Glu Arg1010 1015 1020Val Gly Cys Asp Leu Ile Asp Asp Leu Thr Leu Glu Ala Pro Leu1025 1030 1035Leu Leu Pro Pro Val Gly Ala Val Asp Leu Gln Val Ala Val Gly1040 1045 1050Ala Thr Asp Ala Ala Gly Arg Arg Ala Val Thr Val Tyr Ser Arg1055 1060 1065Pro Ser Gly Gly Gly Ser Glu Gly Trp Glu Ser Ser Ala Asp Pro1070 1075 1080Gly Val Ala Asp Glu Pro Gly Asp Gly Pro Tyr Gly Pro Trp Arg1085 1090 1095Arg His Ala Thr Ala Thr Leu Gly Thr Ala Pro Thr Gly Val Pro1100 1105 1110Glu Pro Ala Ala Ser Pro Ala Gln Trp Pro Pro Ala Gly Ala Glu1115 1120 1125Ala Ile Asp Val Ala Gly Leu Tyr Glu Arg Leu Ala Ala Glu Gly1130 1135 1140Tyr Arg Tyr Gly Pro Ala Phe Thr Gly Leu Arg Thr Ala Trp Arg1145 1150 1155Val Gly Glu Glu Met Phe Ala Glu Val Gly Leu Ala Pro Gly Gln1160 1165 1170Arg Gly Asp Gly Gly Ala Tyr Ala Val His Pro Ala Leu Leu Asp1175 1180 1185Ala Ala Leu His Pro Ile Gly Ala Leu Phe Thr Gly Glu Asp Gln1190 1195 1200Ala Gly Gly Ala Pro Gly Thr Val Arg Leu Pro Phe Ser Phe Gly1205 1210 1215Gly Val Arg Leu Leu Ala Arg Gly Ala Ser Arg Leu Arg Val Arg1220 1225 1230Ile Thr Pro Thr Gly Pro Asp Thr Val Thr Met Arg Leu Ser Asp1235 1240 1245Asp Thr Gly Ala Glu Val Val Ala Val Asp Ser Leu Thr Leu Arg1250 1255 1260Thr Val Ser Ala Gln Arg Trp Arg Ser Gly Ala Val Pro Ala Asp1265 1270 1275Arg Pro Leu Tyr Arg Leu Asp Trp Asp Ala Phe Ala Leu Pro Ala1280 1285 1290Ala Ala Thr Thr Ala Pro Asp Arg Trp Ala Val Leu Ala Ala Asp1295 1300 1305Asp Thr Asn Ala Thr Asp Thr Ala Thr Ala Ser Leu Pro Ala Glu1310 1315 1320His Val Ala Arg His Pro Asp Leu Ala Ala Leu Ser Ala Ser Val1325 1330 1335Ala Ala Gly Ala Pro Ala Pro Asp Leu Val Val Met Ala Cys Leu1340 1345 1350Gly Ala Pro Tyr Asp Thr Ser Asp Asp Gly Asp Glu Pro Pro Ser1355 1360 1365Gln Val Arg Thr Ala Thr His Arg Val Leu Ala Arg Leu Arg Glu1370 1375 1380Trp Leu Thr Asp Asp Ala Leu Ala Ala Ser Arg Leu Val Val Leu
1385 1390 1395Thr Ala Lys Ala Val Ala Ala Asp Pro Ala Asp Ala Pro Pro Asp1400 1405 1410Leu Ala Gly Ala Ala Val Leu Gly Leu Leu Arg Ala Ala Gln Ala1415 1420 1425Glu His Pro Gly Arg Ile Val Leu Val Asp Thr Asp Gly Ile Ser1430 1435 1440Ala Ser Arg Asp Ala Leu Ala Ala Ala Val Ala Ala Ala Val Ala1445 1450 1455Ala Gly Glu Trp Gln Leu Ala Leu Arg Asp Gly Arg Ala Leu Val1460 1465 1470Pro Arg Leu Ile Leu Ala His Pro Asp Pro Asp Ala Ala Pro Val1475 1480 1485Val Leu Asp Pro Asp Gly Thr Val Leu Val Thr Gly Gly Thr Gly1490 1495 1500Ser Leu Gly Arg Leu Leu Ala Arg His Leu Val Glu His His Gly1505 1510 1515Ala Arg His Leu Leu Leu Val Ser Arg Ser Gly Pro Ala Ala Glu1520 1525 1530Gly Ile Glu Ala Phe Ala Ala Gly Leu Ala Ala Asp Val Arg Ile1535 1540 1545Glu Ser Cys Asp Thr Thr Asp Pro Glu Ala Leu Ala Ala Leu Leu1550 1555 1560Ala Thr Val Pro Gly Glu His Pro Leu Thr Ala Val Val His Thr1565 1570 1575Ala Gly Val Leu Asp Asp Gly Val Val Thr Ser Leu Thr Pro Glu1580 1585 1590Gln Leu Asp Thr Val Leu Ala Pro Lys Val Asp Ala Ala Trp Gln1595 1600 1605Leu His Arg Leu Thr Arg Gly Ala Asp Leu Ala Ser Phe Val Leu1610 1615 1620Phe Ser Ser Ala Ala Ser Val Leu Gly Ser Gly Gly Gln Gly Asn1625 1630 1635Tyr Gly Ala Ala Asn Ala Phe Leu Asn Ala Leu Ala Glu His Ile1640 1645 1650Arg Ala Ala Gly Gly Pro Ala Thr Ser Leu Ala Trp Gly Leu Trp1655 1660 1665Gly Val Asp Glu Gly Met Thr Glu His Leu Ala Thr Ala Asp Arg1670 1675 1680Ala Arg Met Ala Arg Ser Gly Thr Ala Ala Met Ser Gly Glu Ala1685 1690 1695Gly Leu Ala Arg Phe Asp Ala Ala Leu Ala Thr Ala Leu Pro Val1700 1705 1710Leu Val Pro Ala Arg Phe Asp Leu Ala Val Leu Arg Glu Gln Ala1715 1720 1725Ala Gly Gly Ala Leu Pro Pro Leu Leu Arg Arg Leu Val Arg Leu1730 1735 1740Pro Val Arg Thr Ala Ala Ala Val Glu Ala Ser Pro Ser Trp Ala1745 1750 1755Gly Arg Leu Ala Gly Leu Pro Glu Thr Glu Gln Asp Arg Val Ile1760 1765 1770Gly Glu Leu Ile Arg Asp Arg Ile Ala Ala Val Leu Ala His Pro1775 1780 1785Glu Pro Glu Thr Leu Glu Leu Gly Arg Thr Phe Ala Gln Leu Gly1790 1795 1800Leu Asp Ser Leu Thr Ala Leu Glu Leu Arg Asn Ala Ile His Glu1805 1810 1815Ala Thr Gly Val Arg Leu Pro Ala Thr Ala Ile Phe Asp Tyr Pro1820 1825 1830Thr Pro Glu Thr Leu Val Ser His Leu Arg Thr Glu Leu Leu Gly1835 1840 1845Ala Thr Ala Thr Thr Ala Ala Thr Ala Pro Leu Pro Pro Gly Ala1850 1855 1860Gly Ala Pro Ala Arg Ser Gly Ser Ala Asp Asp Pro Val Val Ile1865 1870 1875Val Gly Met Ala Cys His Tyr Pro Gly Asp Val His Ser Pro Asp1880 1885 1890Glu Leu Trp Arg Leu Val Ala Asp Gly Val Asp Ala Ile Gly Pro1895 1900 1905Phe Pro Glu Asp Arg Gly Trp Asp Val Ala Gly Leu Tyr Asp Pro1910 1915 1920Asp Pro Glu Arg Thr Gly Lys Ser Tyr Thr Arg Glu Gly Gly Phe1925 1930 1935Leu Pro Glu Ala Ala Leu Phe Asp Ala Glu Phe Phe Gly Ile Ser1940 1945 1950Pro Arg Glu Ala Leu Ala Thr Asp Pro Gln Gln Arg Leu Leu Leu1955 1960 1965Glu Thr Ala Trp Gln Ala Phe Glu His Ala Arg Ile Asp Pro Ala1970 1975 1980Ala Leu Arg Gly Ser Arg Thr Ala Val Val Thr Gly Ile Met Tyr1985 1990 1995Asp Asp Tyr Gly Ala Arg Phe Leu Gly Arg Ile Pro Glu Gly Tyr2000 2005 2010Glu Gly Gln Ile Met Thr Gly Ser Thr Pro Ser Val Ala Ser Gly2015 2020 2025Arg Val Ala Tyr Thr Phe Gly Leu Glu Gly Pro Thr Leu Thr Val
2030 2035 2040Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Met His Leu Ala Ala2045 2050 2055Gln Ala Leu Arg Gln Gly Glu Cys Asp Leu Ala Leu Ala Gly Gly2060 2065 2070Val Thr Val Met Ala Thr Pro Asn Thr Phe Ile Glu Phe Ser Arg2075 2080 2085Gln Arg Gly Leu Ala Pro Asp Gly Arg Cys Lys Pro Phe Ala Ala2090 2095 2100Ala Ala Asp Gly Thr Gly Trp Ser Glu Gly Ala Gly Leu Leu Val2105 2110 2115Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val Leu2120 2125 2130Ala Val Leu Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn2135 2140 2145Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Gly2150 2155 2160Gln Ala Leu Ala Ala Ala Gly Val Asp Pro Ala Gly Val Asp Val2165 2170 2175Val Glu Ala His Gly Thr Gly Thr Met Leu Gly Asp Pro Ile Glu2180 2185 2190Ala Gln Ala Leu Leu Ala Thr Tyr Gly Arg Asn Arg Pro Ala Glu2195 2200 2205Gln Pro Leu Trp Leu Gly Ser Ile Lys Ser Asn Ile Gly His Thr2210 2215 2220Gln Ala Ala Ala Gly Ala Ala Gly Ile Ile Lys Met Val Met Ala2225 2230 2235Leu Arg His Gly Arg Leu Pro Ala Thr Leu His Val Asp Glu Pro2240 2245 2250Ser Pro His Val Asp Trp Ala Ser Gly Ser Val Arg Leu Leu Thr2255 2260 2265Glu Ala Thr Asp Trp Pro Glu Ala Asp Arg Pro Arg Arg Ala Ala2270 2275 2280Val Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His Leu Ile Leu2285 2290 2295Glu Gln Ala Pro Asp Gln Pro Glu Pro Leu Pro Glu Gln Ser Glu2300 2305 2310Ser Ala Thr Ala Gly Gly Ile Val Pro Phe Val Leu Ser Ala Arg2315 2320 2325Thr Ala Glu Ala Leu Arg Ala Gln Ala Ala Asn Leu Ala Ala Arg2330 2335 2340Leu Pro Ser Ala Gly Val Ala Glu Val Gly Trp Ser Leu Ala Thr2345 2350 2355Thr Arg Ser Ala Phe Glu His Arg Ala Val Ile Val Ala Glu Asp2360 2365 2370Arg Asp Ala Leu Leu Ala Gly Leu Glu Lys Leu Ala Ala Asp Glu2375 2380 2385Pro Asp Pro Ala Val Val Ala Gly Thr Ala Thr Thr Ala Ala Ala2390 2395 2400Gly Pro Val Phe Val Phe Pro Gly Gln Gly Ser Gln Trp Arg Gly2405 2410 2415Met Gly Val Glu Leu Leu Asp Thr Ser Pro Val Phe Ala Ala Arg2420 2425 2430Ile Ala Glu Cys Glu Arg Ala Leu Ala Pro Tyr Val Asp Trp Ser2435 2440 2445Leu Thr Ala Val Leu Arg Gly Ser Asp Thr Thr Thr Asp Pro His2450 2455 2460Arg Val Asp Val Val Gln Pro Thr Leu Trp Ala Val Met Val Ser2465 2470 2475Leu Ala Ala Leu Trp Gln His Leu Gly Ile Ala Pro Ala Ala Val2480 2485 2490Ile Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val Ala Gly2495 2500 2505Ala Leu Thr Leu Asp Asp Ala Ala Lys Val Val Ala Leu Arg Ser2510 2515 2520Gln Ala Leu Arg Ala Leu Ala Gly His Gly Thr Met Ala Ser Leu2525 2530 2535Thr Leu Gly Ala Asp Asp Thr Ala Gly Leu Leu Glu Glu Leu Gly2540 2545 2550Glu Arg Ala Asp Asp Val Thr Val Ala Ala Ala Asn Gly Pro Val2555 2560 2565Thr Thr Val Ile Ser Gly Ala Val Glu Gln Ile Ala Thr Val Leu2570 2575 2580Ala Ala Ala Glu Ala His Gly Ala Arg Thr Arg Thr Ile Asp Val2585 2590 2595Asp Tyr Ala Ser His Gly Pro His Val Asp Arg Ile Arg Glu Asp2600 2605 2610Ile Val Ser Ala Leu Ser Gly Leu Ala Pro Thr Ala Ser Glu Val2615 2620 2625Ala Phe Tyr Ser Thr Val Thr Ala Glu Arg Leu Asp Thr Ala Gly2630 2635 2640Leu Asp Ala Asp Tyr Trp Phe Thr Asn Leu Arg Arg Pro Val Arg2645 2650 2655Phe Ala Asp Thr Leu Ala Thr Leu Leu Ala His Gly His Arg His2660 2665 2670Phe Ile Glu Val Ser Pro His Pro Val Leu Ile Pro Gly Met Gln
2675 2680 2685Asp Gly Phe Glu Ala Ala Asp Ala Ala Ala Thr Ala Val Pro Thr2690 2695 2700Leu Arg Arg Asp Gln Gly Gly Pro His Gln Leu Ala Gln Ala Val2705 2710 2715Ala Arg Ala Tyr Thr Ala Gly Leu Ala Ile Asp Trp Ala Pro Trp2720 2725 2730Tyr Pro Ala Arg Pro Tyr Thr Thr Asp Leu Pro Thr Tyr Pro Phe2735 2740 2745Gln Arg Arg Arg Tyr Trp Leu Gly Met Asp Gly Gly Pro Gly Asp2750 2755 2760Leu Arg Ser Ala Gly Leu Val Ser Val Ser His Ala Gln Ile Gly2765 2770 2775Ala Ala Val Glu Leu Ala Asp Gly Gly Leu Val Met Thr Gly Arg2780 2785 2790Leu Pro Ala Ala Gly Ser Gly Gly Trp Leu Asp Asp His Val Val2795 2800 2805Ala Asp Thr Pro Leu Val Pro Gly Thr Ala Leu Val Glu Trp Val2810 2815 2820Leu Arg Ala Ala Asp Glu Ala Gly Cys Gly Gly Ile Glu Glu Leu2825 2830 2835Ala Leu His Val Pro Met Thr Leu Pro Ala Ser Gly Gly Leu Arg2840 2845 2850Ile Gln Val Val Ala Tyr Ala Pro Asp Gly Asp Gly Arg Arg Glu2855 2860 2865Val Arg Val His Ser Arg Pro Asp Ala Glu Asp Gly Ser Ser Pro2870 2875 2880Trp Thr Cys His Ala Thr Gly His Leu Ser Pro Thr Ala Pro Gly2885 2890 2895Ala Ala Asp Pro Ala Pro Ala Gly Val Trp Pro Pro Arg Asp Ala2900 2905 2910Glu Gln Val Asp Val Ala Asp Phe Tyr Gly Arg Ala Glu Ala Ile2915 2920 2925Gly Tyr Gly Tyr Gly Pro Ala Phe Arg Gly Leu Thr Ala Ala Trp2930 2935 2940Arg Gln Gly Asp Asp Leu Leu Ala Glu Val Val Leu Pro Glu Ala2945 2950 2955Ala His Glu Gly Ala Asp Gly Phe Ala Leu His Pro Ala Leu Leu2960 2965 2970Asp Ala Ala Leu His Pro Leu Ala Leu Asp Gly Gln Gly Glu Asp2975 2980 2985Gly Arg Met Arg Leu Pro Phe Ala Trp Ser Gly Val Ser Leu Trp2990 2995 3000Ala Thr Gly Ala Arg Ala Ala Arg Val Arg Met Ser Pro Leu Glu3005 3010 3015His Gly Phe Arg Leu Val Val Ala Asp Ala Ala Gly Arg Pro Val3020 3025 3030Leu Ser Ala Glu Ser Val Val Val Arg Pro Thr Ser Ala Arg Gln3035 3040 3045Leu Arg Asp Ala Gly Ala Arg Arg Val Asp Gly Leu Tyr Glu Val3050 3055 3060Ala Trp Val Ala Leu Pro Pro Ser Ser Asp Thr Val Ala Glu Thr3065 3070 3075Glu Thr Arg Gly Val Glu Gly Trp Ala Leu Leu Asp Gly Gly Pro3080 3085 3090Leu Pro Phe Asp Pro Ser Lys Ala Gly Ser Leu Pro Arg His Ala3095 3100 3105Asp Ile Asp Ala Leu Leu Thr Ala Pro Ala Leu Pro Ser Thr Val3110 3115 3120Leu Val Gly Val Ser Gly Pro Val Gly Ala Ala Gly Asp Glu Asn3125 3130 3135Ser Ala Glu Gly Ala Leu Ala Val Thr Thr Gly Val Leu Thr Ser3140 3145 3150Ala Arg Arg Trp Leu Glu Thr Pro Glu Leu Ala Asp Ala Arg Leu3155 3160 3165Val Leu Val Thr Arg Gly Ala Val Ala Ala Ala Glu Thr Asp Asp3170 3175 3180Gly Pro Asp Pro Ala Ala Ala Ala Val Trp Gly Leu Leu Arg Ser3185 3190 3195Ala Gln Ala Glu Asn Pro Gly Arg Phe Leu Leu Cys Asp Ile Asp3200 3205 3210Asp Gly Ala Gly Pro Asp Asp Val Leu Gly Ala Val Thr Arg Ala3215 3220 3225Val Ala Leu Asp Glu Pro Gln Val Ala Val Arg Gly Glu Arg Val3230 3235 3240Leu Thr Pro Arg Leu Glu Arg Ala Gly Ala Ala Glu Leu Val Pro3245 3250 3255Pro Pro Gly Glu Pro Ala Trp Arg Leu Ser Ala Asp Asp Thr Gly3260 3265 3270Thr Ile Asp Ser Val Ser Val Val Ala Cys Pro Glu Val Leu Glu3275 3280 3285Pro Leu Ala Pro Gly Gln Val Arg Ile Ala Val Arg Ala Ala Gly3290 3295 3300Ile Asn Phe Arg Asp Val Leu Ile Val Leu Gly Met Tyr Pro Asp3305 3310 3315Glu Gly Val Phe Arg Gly Ser Glu Gly Ala Gly Val Val Leu Asp
3320 3325 3330Val Ala Asp Asp Val Thr Ser Val Ala Val Gly Asp Arg Val Phe3335 3340 3345Gly Leu Phe Glu Gly Ala Phe Gly Pro Val Ala Val Ala Asp Ala3350 3355 3360Arg Ala Val Val Pro Val Pro Pro Asp Trp Thr Asp Gln Gln Ala3365 3370 3375Ala Ala Val Pro Thr Thr Phe Leu Thr Ala Trp Tyr Gly Leu Val3380 3385 3390Asp Leu Ala Gly Leu Lys Ala Gly Glu Ser Val Leu Ile His Ala3395 3400 3405Ala Thr Gly Gly Val Gly Thr Ala Ala Val Gln Ile Ala Arg His3410 3415 3420Leu Gly Ala Glu Val Tyr Ala Thr Ala Gly Pro Gly Lys His Ala3425 3430 3435Val Leu Glu Ala Met Gly Ile Asp Glu Ala His Arg Ala Ser Ser3440 3445 3450Arg Asp Leu Asp Phe Glu Asp Ala Phe Arg Thr Ala Thr Gly Gly3455 3460 3465Arg Gly Val Asp Val Ile Leu Asn Ser Leu Ala Gly Glu Tyr Thr3470 3475 3480Asp Ala Ser Leu Arg Leu Leu Thr Gly Gly Gly Arg Phe Ile Glu3485 3490 3495Met Gly Lys Thr Asp Lys Arg Asp Ala Glu Gln Ile Ala Asp Thr3500 3505 3510Tyr Ser Gly Val Arg Tyr Arg Phe Tyr Asp Leu Val Pro Asp Ala3515 3520 3525Gly Leu Asp Arg Val Ala Glu Met Leu Thr Thr Leu Ala Gly His3530 3535 3540Phe Ala Gln Gly Val Leu Ala Pro Pro Pro Val Arg Ala Trp Pro3545 3550 3555Leu Thr Glu Ala Arg Gln Ala Leu Arg Gln Met Ser Gln Ala Arg3560 3565 3570His Thr Gly Lys Tyr Val Leu Asp Met Pro Arg Thr Leu Asp Pro3575 3580 3585Asp Gly Thr Val Leu Ile Thr Gly Gly Thr Gly Thr Leu Gly Ala3590 3595 3600Leu Val Ala Glu His Leu Val Thr Asn His His Ile Ser His Leu3605 3610 3615His Leu Leu Ser Arg Arg Gly Pro Asp Ala Pro Gly Ser Ala Asp3620 3625 3630Leu Ala Ala Arg Leu Thr Glu Leu Gly Ala Thr Val Arg Ile Thr3635 3640 3645Ala Thr Asp Thr Thr Asp Pro Gln Ala Leu Arg Gln Ala Leu Asp3650 3655 3660Thr Val Asp Arg Asp His Pro Leu Thr Gly Val Ile His Ala Ala3665 3670 3675Gly Ala Leu Asp Asp Ala Val Leu Thr Ala Gln Thr Pro Glu Arg3680 3685 3690Leu Ala Ser Val Trp Ala Ala Lys Ala Thr Ala Ala Ala Asn Leu3695 3700 3705His Arg Ala Thr Lys Asp Leu Pro Leu Ala Met Phe Val Ile Phe3710 3715 3720Ser Ser Ala Ala Gly Thr Leu Gly Thr Pro Gly Gln Ala Asn Tyr3725 3730 3735Ala Ala Ala Asn Ala Tyr Cys Asp Ala Leu Ala Val Arg Arg Arg3740 3745 3750Arg Ala Gly Leu Pro Ala Thr Ser Ile Ala Trp Gly Leu Trp Ala3755 3760 3765Ala Thr Ser Glu Met Thr Gly His Leu Ala Asp Ala Asp Leu Ala3770 3775 3780Arg Met Ser Arg Thr Gly Phe Thr Pro Leu Ala Thr Pro Met Ala3785 3790 3795Leu Ala Leu Phe Asp Ala Ala Gly Arg His Gly Ala Ala Thr Pro3800 3805 3810Leu Ala Leu Asp Leu Asp Pro Arg Thr Leu Gly Ala Gln Pro Ser3815 3820 3825Asp Ala Val Pro Ala Val Leu Arg Thr Val Ala Ala Ala Gly Ala3830 3835 3840Pro Val Arg Arg Thr Ala Ala Val Ala Gln Ser Thr Asp Trp Ala3845 3850 3855Gly Arg Leu Ala Ala Leu Ser Ala Ala Glu Arg His Arg Glu Leu3860 3865 3870Val Asn Leu Val Arg Thr His Ala Ala Thr Val Leu Gly His Ser3875 3880 3885Asp Pro Ala Ala Leu Arg Ala Asp Thr Ser Phe Lys Glu Leu Gly3890 3895 3900Phe Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Ser Ala3905 3910 3915Ala Thr Gly Leu Arg Leu Pro Ala Ala Leu Val Phe Asp Tyr Pro3920 3925 3930Asp Ala Glu Thr Met Ala Arg Phe Leu Asp Gln Lys Leu Ala Pro3935 3940 3945Gly Asp Arg Thr Glu Ala Ala Ala Val Asp His Leu Ala Pro Val3950 3955 3960Leu Asn Asp Leu Ala Arg Leu Glu Ser Thr Leu Gly Ser His Asp
3965 3970 3975Val Asp Gly Lys Ala Arg Glu Thr Val Ala Gly Arg Leu His Ala3980 3985 3990Leu Leu Ser Arg Leu Glu Gly Ser Thr Ala Ser Ala Ala Asp Ile3995 4000 4005Asp Gly Glu Ala Leu Glu Ser Ala Ser Asp Asp Glu Met Phe Ala4010 4015 4020Leu Ile Asp Gln Gln Leu Gly Ser Ser4025 4030<210>12<211>3956<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met Ser Ser Thr Glu Asp Lys Leu Arg Gln Tyr Leu Lys Arg Val Thr1 5 10 15Val Asp Leu Gly Glu Ala Arg Ala Arg Leu Arg Lys Ala Glu Gln Arg20 25 30Gln His Glu Pro Ile Ala Ile Thr Ser Met Ala Cys Arg Tyr Pro Gly35 40 45Gly Val Thr Ser Pro Glu Thr Leu Trp Glu Leu Val Asp Ser Arg Thr50 55 60Asp Ala Ile Gly Ser Phe Pro Ala Asn Arg Gly Trp Asn Leu Ala Ser65 70 75 80Leu Tyr His Pro Asp Pro Asp His Ser Gly Thr Ser Tyr Val Arg Asp85 90 95Gly Gly Phe Val His Asp Ala Asp Glu Phe Asp Ala Ser Phe Phe Asn100 105 110Ile Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu115 120 125Leu Glu Thr Ala Trp Glu Leu Leu Glu Arg Ala His Ile Asp Pro Thr130 135 140Ala Leu Lys Gly Thr Pro Thr Gly Val Tyr Thr Gly Cys Gly Val Pro145 150 155 160Gly Phe Gly Thr Pro His Ile Glu Arg Ser Ala Glu Gly Phe Leu Leu165 170 175Thr Gly Asn Ala Leu Ser Val Val Ser Gly Arg Ile Ala Phe Thr Leu180 185 190Gly Leu Glu Gly Pro Ala Val Thr Leu Asp Thr Ala Cys Ser Ser Ser195 200 205Leu Val Ala Met His Leu Ala Val Gln Ala Leu Arg Gln Gly Glu Cys210 215 220Asp Leu Ala Leu Ala Gly Gly Val Thr Val Met Ser Thr Pro Asn Val225 230 235 240Ile Val Glu Phe Ser Arg Gln Arg Gly Leu Ser Pro Asp Gly Arg Cys245 250 255Lys Pro Phe Ala Thr Ala Ala Asp Gly Thr Gly Phe Ser Glu Gly Ala260 265 270Gly Leu Val Leu Leu Glu Arg Leu Ser Asp Ala Glu Arg Asn Gly His275 280 285Gln Val Leu Ala Val Ile Arg Gly Thr Ala Val Asn Gln Asp Gly Ala290 295 300Ser Asn Gly Leu Ser Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile305 310 315 320Arg Gln Ala Leu Ala Asn Ala Gly Leu Ala Thr Val Glu Val Asp Ala325 330 335Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu Ala340 345 350Glu Ala Leu Leu Ala Thr Tyr Gly Gln Glu Arg Pro Glu Asp Arg Pro355 360 365Leu Trp Leu Gly Ser Ile Lys Ser Asn Ile Gly His Thr Gln Gly Ala370 375 380Ala Gly Val Ala Gly Val Ile Lys Met Val Met Ala Met Arg His Ala385 390 395 400Ser Leu Pro Ala Thr Leu His Val Asp Glu Pro Thr Ser His Val Asp405 410 415Trp Asp Arg Gly Thr Val Arg Leu Leu Thr Glu Pro Val Asp Trp Pro420 425 430Thr Ala Pro Asp Arg Pro Arg Arg Ala Gly Val Ser Ala Phe Gly Ile435 440 445Ser Gly Thr Asn Ala His Ile Ile Leu Glu Glu Ala Gly Leu Pro Thr450 455 460Ala Ala Glu Ala Glu Ala Gly Thr Ala Ala Glu Ala Gly Thr Asp Ala465 470 475 480Gly Ala Gly Thr Glu Ala Gly Ala Glu Asp Ala Ala Pro Glu Glu Ala485 490 495Thr Val Glu Pro Ala Leu Leu Gly Gly Val Ala Pro Trp Val Val Ser500 505 510Ala Arg Thr Gln Glu Ala Leu Ala Asp Gln Ala Arg Gly Leu Val Arg515 520 525Ala Val Thr Asp Thr Gly Ala Pro Asp Ala Val Pro Ala Glu Val Ala530 535 540Trp Ser Leu Ala Thr Thr Arg Ala Thr Phe Asp His Arg Ala Val Val545 550 555 560Thr Gly Thr Glu Leu Ala Asp Leu Thr Ala Ala Leu Glu Ala Leu Ala565 570 575Thr Gly Gly Glu His Pro His Leu Val Arg Gly Thr Ala Leu Asp Pro580 585 590Gln Ala Gly Pro Val Leu Val Phe Pro Gly Gln Gly Ser Gln Trp Pro595 600 605Gly Met Ala Val Gly Leu Leu Asp Ser Ser Pro Ala Phe Ala Thr Arg610 615 620Ile Ala Ala Cys Glu Gln Ala Leu Ala Pro Tyr Val Asp Trp Ser Leu625 630 635 640Thr Ala Val Leu Arg Gly Ser Asp Thr Ala Thr Asp Pro His Arg Val645 650 655Asp Val Ile Gln Pro Thr Leu Trp Ala Val Met Val Ser Leu Ala Gly660 665 670Leu Trp Gln Asp Phe Gly Ile Thr Pro Ala Ala Val Ile Gly His Ser675 680 685Gln Gly Glu Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Ser Leu Asp690 695 700Asp Ala Ala Lys Val Val Ala Leu Arg Ser Gln Ala Leu Arg Ala Leu705 710 715 720Ala Gly His Gly Ala Met Ala Ser Leu Thr Leu Gly Ala Glu Asp Thr725 730 735Ala Arg Val Leu Thr Gly Leu Gly Pro Ala Ala Glu Gly Val Ala Val740 745 750Ala Ala His Asn Gly Pro Arg Ser Thr Val Val Ser Gly Pro Pro Asp
755 760 765Gln Ile Ala Thr Val Leu Ala Ala Ala Glu Ala Arg Gly Ala Arg Thr770 775 780Arg Thr Ile Asp Val Asp Tyr Ala Ser His Ser Pro His Val Asp Arg785 790 795 800Ile Arg Asp Thr Ile Leu Ala Gln Leu Ala Asp Leu Ala Pro Ala Ala805 810 815Pro Thr Ile Pro Phe Tyr Ser Thr Val Thr Gly Glu Pro Leu Ala Asp820 825 830Thr Pro Leu Asp Ala Glu Tyr Trp Phe Thr Asn Leu Arg Gln Pro Val835 840 845Arg Phe Thr Asp Thr Leu Thr Thr Leu Leu Asp His Gln His Arg His850 855 860Phe Ile Glu Ala Ser Pro His Pro Val Leu Thr Pro Gly Ile Gln Asp865 870 875 880Ala Ile Asp Asp Ala Glu Leu Pro Ala Thr Thr Ile Pro Thr Leu Arg885 890 895Arg Asp His Gly Thr Pro His Asp Leu Ala Asp Ala Leu Ala Leu Ala900 905 910His Thr Thr Gly Leu Ala Val Asp Trp Arg Pro Trp Tyr Ala Thr Thr915 920 925Pro Pro Ala Thr Thr Asp Leu Pro Thr Tyr Pro Phe Gln Arg Gln Arg930 935 940Tyr Trp Ser Ala Ala Gly Arg Arg Thr Gly Asp Val Ser Ala Ala Gly945 950 955 960Leu Arg Pro Val Asp His Pro Gln Leu Ser Ala Ala Thr Gly Leu Ala965 970 975Asp Gly Gly Leu Leu Leu Thr Gly Arg Leu Pro Ala Ala Gly Asp Ala980 985 990Gly Trp Leu Gly Glu His Glu Phe Ala Asp Val Val Leu Val Pro Ser995 1000 1005Thr Ala Leu Val Glu Trp Thr Leu Arg Ala Ala Asp Glu Ala Gly1010 1015 1020Cys Gly Gly Val Glu Glu Leu Thr Leu Glu Val Pro Leu Thr Leu1025 1030 1035Ser Ala Ala Ser Glu Leu Arg Val Gln Val Val Val Asp Ala Pro1040 1045 1050Asp Glu Asp Gly Arg Arg Ala Val Arg Val Ser Ser Gln Pro Ala1055 1060 1065Val Asp Thr Pro Asp Arg Ala Asp Gly Gln Asp Thr Trp Thr Cys1070 1075 1080His Ala Thr Gly Thr Leu Met Ala Ala Ala Ala Ala Gly Thr Glu1085 1090 1095Leu Ala Gly Ala Trp Pro Pro Ala Gly Ala Glu Pro Val Asp Leu1100 1105 1110Thr Asn Leu Tyr Ala Arg Ala Glu Ala Ala Gly Tyr Arg Tyr Gly1115 1120 1125Pro Thr Phe Gln Gly Val Gln Ala Val Trp Arg His Gly Ala Asp1130 1135 1140Leu Leu Ala Glu Val Ala Leu Asp Gln Gly Ala Glu Glu Gly Gly1145 1150 1155Asp Glu Phe Gly Ile His Pro Ala Leu Leu Glu Cys Ala Leu His1160 1165 1170Pro Val Ala Leu Thr Asp Thr Pro His Asp Asp Thr Pro Leu Gly1175 1180 1185Asp Ala Asp Thr Asp Gly Pro Leu Trp Leu Pro Phe Ala Trp Asn1190 1195 1200Gly Val Ser Leu His Ala Gly Gly Ala Thr Ser Val Arg Val Arg1205 1210 1215Ile Gly Gln Arg Gly Gln Thr Asp Thr Glu Gly Arg Glu Leu Thr1220 1225 1230Val Val Val Ala Asp Pro Thr Gly Ala Pro Val Leu Thr Val Asp1235 1240 1245Ser Val Ala Leu Arg Pro Ala Asp Gly Asp Trp Leu Lys Ala Ala1250 1255 1260Glu Arg Arg Ser Thr Ala Ala Leu Phe Thr Val Glu Trp Thr Pro1265 1270 1275Leu Pro Pro Gln Asp Ser Arg Pro Glu Pro Val Glu Ala Glu Asp1280 1285 1290Gly Trp Ala Thr Leu Gly Ala Ser Gly Pro Gly His His Tyr Ala1295 1300 1305Asp Leu Ala Ala Leu Leu Ser Ala Ala Asp Gly Ala Glu Pro Ala1310 1315 1320Pro Pro Val Val Leu Ala Ser Val Thr Pro Thr Ala Asp Thr Gly1325 1330 1335Ala Asp Ser Glu Ala Asp Thr Asp Leu Ala Thr Val Arg Arg Thr1340 1345 1350Leu Gly Leu Ile Gln Glu Trp Leu Ala Glu Pro Gly Leu Arg Asp1355 1360 1365Ser Arg Leu Val Leu Ile Thr Ser Gly Ala Thr Ser Val Gly Asp1370 1375 1380Gly Asp Gly Pro Val Glu Pro Gly Ser Ala Ala Val Phe Gly Leu1385 1390 1395Val Gln Ala Val Gln Ala Glu His Pro Asp Arg Phe Met Leu Val1400 1405 1410Asp Val Gly Ala Asp Ala Asp Ala Asp Gly Asp Gly Asp Gly Gly
1415 1420 1425Glu Thr Leu Ala Asp Ala Val Arg Arg Ala Ile Ala Ala Asp Glu1430 1435 1440Pro Gln Ile Ala Val Arg Ser Gly Glu Val Ser Val Pro Arg Leu1445 1450 1455Leu Arg Ala Ala Ala Arg Pro Asp Glu Gly Thr Ala Val Glu Leu1460 1465 1470Ser Gly Gly Thr Val Val Val Ser Gly Ala Met Asp His Val Ser1475 1480 1485Gly Gly Ala Ile Ala Glu Gln Leu Val Arg Ala Tyr Gly Ala Glu1490 1495 1500Arg Leu Leu Leu Leu Ser His Pro Asp Glu Gln Ala Pro Asp Leu1505 1510 1515Ala Glu Arg Leu Thr Ala Leu Gly Ala Ala Val Glu Val Ala Val1520 1525 1530Val Asp Ile Ala Asp Arg Ala Ala Leu Ala Glu Val Leu Ala Ser1535 1540 1545Val Pro Asp Ser His Pro Leu Val Gly Val Val His Leu Ala Gly1550 1555 1560Ala Ala Asp Glu Gly Pro Val Glu Ser Trp Asn Asp Gly Arg Leu1565 1570 1575Ser Arg Ala Trp Ala Pro Arg Ala Thr Gly Ala Trp Gln Leu His1580 1585 1590Thr Leu Thr Gln Asp Leu Pro Leu Arg Met Phe Val Val Cys Ser1595 1600 1605Ala Ala Ala Asp Val Thr Gly Gly Pro Gly Arg Ala Gly Tyr Ala1610 1615 1620Ala Ala Asn Ala His Thr Asp Ala Leu Ile Ala His Arg Arg Ala1625 1630 1635Ala Gly Leu Pro Gly Thr Gly Leu Val Trp Ala Leu Glu Glu Glu1640 1645 1650Ala Thr Ala Asp Ala Ser Arg Leu Phe Asp Ala Ala Phe His Ala1655 1660 1665Val Gln Pro Leu Val Val Ala Ala Asp Leu Asp Thr Ala Arg Leu1670 1675 1680Gly Pro Ser Ala Pro Ala Leu Leu Arg Ala Leu Val Arg Pro Ala1685 1690 1695Arg Arg Arg Ala Ala Glu Arg Gln Ser Ala Ala His Ala Leu Thr1700 1705 1710Ser Arg Leu Ala Gly Leu Asp Asn Ser Gly Gln Arg Glu Leu Leu1715 1720 1725Leu Asp Val Val Arg Gln Met Ala Ala Val Val Leu Gly His Ser1730 1735 1740Ser Asp Thr Ala Ile Arg Ala Glu Ala Ala Phe Lys Glu Leu Gly1745 1750 1755Phe Asp Ser Leu Thr Ala Val Gly Leu Arg Asn Arg Leu Val Asp1760 1765 1770Ala Thr Gly Leu Arg Leu Pro Ser Thr Leu Val Phe Asp Tyr Pro1775 1780 1785Thr Pro Arg Ala Leu Ala Asp His Leu Leu Gln Leu Val Thr Ser1790 1795 1800Thr Ala Pro Thr Thr Ser Leu Pro Val Gly Pro Ala Arg Ala Ala1805 1810 1815Gly Ala Asp Asp Glu Pro Ile Ala Val Val Ala Met Ala Cys Arg1820 1825 1830Phe Pro Gly Asp Val Thr Thr Pro Glu Gly Leu Trp Asp Leu Val1835 1840 1845Ala Ala Gly Glu Asn Ile Arg Gly Pro Phe Pro Thr Asn Arg Gly1850 1855 1860Trp Asp Leu Ala Asn Leu Phe His Pro Asp Pro Glu His Pro Gly1865 1870 1875Thr Thr Tyr Ala Ser Gln Gly Ala Phe Ile Tyr Asp Ala Asp Gly1880 1885 1890Phe Asp Ala Ala Phe Phe Gly Ile Asn Pro Arg Glu Ala Leu Ala1895 1900 1905Ile Asp Pro Gln Gln Arg Leu Ile Leu Glu Thr Ala Trp Glu Ala1910 1915 1920Leu Glu Arg Ala Gly Ile Asp Pro His Thr Leu Lys Glu Ser Leu1925 1930 1935Thr Gly Val Tyr Thr Gly Val Ile Tyr His Asp Tyr Ala Ala Gly1940 1945 1950Leu Pro Ala Ser Asp Pro Arg Leu Asp Gly Tyr Thr Met Leu Ser1955 1960 1965Ser Ile Gly Ser Ile Ile Ser Gly Arg Val Ala Tyr Thr Leu Gly1970 1975 1980Leu Gln Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser1985 1990 1995Leu Val Ala Met His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu2000 2005 2010Cys Asp Leu Ala Leu Ala Gly Gly Val Thr VaL Met Ala Thr Pro2015 2020 2025Asp Pro Phe Thr Gly Phe Ser Arg Gln Arg Gly Leu Ala Pro Asp2030 2035 2040Gly Arg Cys Lys Pro Phe Ala Ala Ala Ala Asp Gly Thr Ser Leu2045 2050 2055Ser Glu Gly Ala Gly Leu Val Val Leu Glu Arg Leu Ser Asp Ala
2060 2065 2070Arg Arg Asn Gly His Gln Val Leu Ala Val Leu Arg Gly Ser Ala2075 2080 2085Ile Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly2090 2095 2100Pro Ser Gln Gln Arg Val Ile Gly Gln Ala Leu Ala Asn Ala Gly2105 2110 2115Leu Gly Pro Ala Asp Ile Asp Ala Val Glu Ala His Gly Thr Gly2120 2125 2130Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr2135 2140 2145Tyr Gly Gln His Arg Ala Asp Asp Arg Pro Leu Trp Leu Gly Ser2150 2155 2160Val Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ser Gly Val Val2165 2170 2175Gly Val Ile Lys Met Ile Met Ala Met Arg His Gly Arg Leu Pro2180 2185 2190Ala Ser Leu His Ile Asp Glu Pro Ser Pro His Ile Asp Trp Thr2195 2200 2205Ser Gly Asn Val Gln Leu Leu Thr Glu Ala Ile Asp Trp Pro Glu2210 2215 2220Ala Asp Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Ala Ser2225 2230 2235Gly Thr Asn Ala His Val Ile Leu Glu Glu Ala Pro Pro Pro Pro2240 2245 2250Asp Pro Ala Pro Glu Pro Ala Ala Ala Pro Ala Ile Ala Gly Gly2255 2260 2265Val Val Pro Trp Pro Leu Ser Ala Arg Asp Glu Gln Ala Leu Arg2270 2275 2280Glu Gln Ala Ser Ala Leu Ala Glu His Leu Gly Thr Asp Asp Arg2285 2290 2295Ala Ser Val Ala Asp Val Gly Trp Ser Leu Ala Thr Thr Arg Ala2300 2305 2310Met Phe Glu Arg Arg Ala Val Ile Val Gly Glu Gly Arg Glu Glu2315 2320 2325Met Ala Ala Ala Leu Glu Ala Leu Ala Asp Gly Ser Pro His Pro2330 2335 2340Gly Leu Ser Thr Leu Gly Gly Thr Ala Ser Asp Thr Pro Gly Lys2345 2350 2355Thr Val Trp Leu Phe Ser Gly Gln Gly Ser Gln Arg Pro Gly Met2360 2365 2370Gly Ala Asp Leu Tyr Arg Arg Phe Pro Val Phe Ala Glu Ala Phe2375 2380 2385Asp Gly Val Arg Ala Leu Leu Asp Pro His Leu Asp His Pro Leu2390 2395 2400Ala Asp Val Val Phe Ala Thr Asp Pro Gly His Gly Asp Leu Ile2405 2410 2415His His Thr Thr Tyr Thr Gln Ala Gly Leu Phe Ala Leu His Ile2420 2425 2430Ala Leu Ala Arg Leu Leu Gly Asp Met Gly Leu Ala Pro Asp Ala2435 2440 2445Val Ala Gly His Ser Ile Gly Glu Ile Ser Ala Ala His Leu Ala2450 2455 2460Gly Val Leu Ser Leu Glu Asp Ala Ala Gln Leu Val Ala Ala Arg2465 2470 2475Ala Thr Leu Met Gly Gly Leu Pro Ser Gly Gly Ala Met Ala Thr2480 2485 2490Val Asn Ala Asp Glu Gln Glu Ile Thr Ala Thr Leu Ala Asp Tyr2495 2500 2505Pro Asp Leu Ala Ile Ala Ala Met Asn Thr Pro Ala His Thr Val2510 2515 2520Val Ser Gly Pro Ala Asp Gln Val Ala Ala Leu Thr Ala Ala Trp2525 2530 2535Arg Glu Arg Gly Arg Lys Thr Arg Ala Leu Pro Val Ser His Ala2540 2545 2550Phe His Ser Pro Gln Met Glu Pro Ile Leu Val Pro Phe Thr Glu2555 2560 2565Ala Ile Gly His Leu Ala Phe His Pro Pro Arg Ile Pro Leu Ile2570 2575 2580Ser Asn Leu Thr Gly Glu Pro Ala Gly Glu Asp Ile Ala Thr Pro2585 2590 2595Asp Tyr Trp Ala Arg His Ile Arg Arg Pro Val His Phe His Gln2600 2605 2610Ser Ile Thr His Leu Ala Glu Asp Thr Ala Val Phe Leu Glu Leu2615 2620 2625Gly Pro Ala Pro Val Leu Thr His Ala Val His His Thr Leu Pro2630 2635 2640Glu Glu Thr Thr Ala Thr Ala Leu Ala Thr Leu Thr Gly Lys Gln2645 2650 2655Pro Asp Val Pro Ala Leu Ala His Ser Leu Ala Ala Leu His Thr2660 2665 2670Ser His Ala Pro Val Asp Trp Thr Pro Trp Phe Arg Thr Asp Pro2675 2680 2685Ala Pro Arg Thr Val Gly Leu Pro Thr Tyr Arg Phe Gln Arg Arg2690 2695 2700Pro Tyr Trp Ile Ala Pro Arg Val Ser Gly Gly Ala Thr Pro Gly
2705 2710 2715Gly Thr Gly Leu Asp His Pro Leu Leu Asp Thr Ala Ala Ala Leu2720 2725 2730Ala Asp Gly Gly Met Val Leu Thr Gly Ser Val Pro Pro Ala Asp2735 2740 2745His Asp Ser Trp Leu Thr Glu Arg Ala Ile Ala Gly Thr Val Val2750 2755 2760Leu Pro Gly Thr Ala Leu Leu Glu Leu Ala Leu Arg Cys Ala Glu2765 2770 2775Asp Thr Arg Ser Pro His Val Glu Glu Leu Leu Leu His His Pro2780 2785 2790Leu Thr Leu His Pro Thr Ala His Leu Asp Leu Gln Val Val Ile2795 2800 2805Gly Ala Ala Asp Asp Asp Ala Arg Arg Thr Leu His Leu Tyr Thr2810 2815 2820Arg Pro Gln Ser Asp Ser Ser Ala Glu Trp Thr Arg His Ala Thr2825 2830 2835Ala Thr Leu Thr Gly Glu Pro Thr Asp Asp Arg Pro Pro Ala Glu2840 2845 2850Gly Glu Ala Ala Trp Pro Pro Ala Gly Ala Glu Pro Val Asp Leu2855 2860 2865Thr Gly Phe Tyr Asp Arg Ala Ala Ser Asn Gly Tyr Ala Tyr Gly2870 2875 2880Pro Ser Leu Arg Gly Leu Gln Ala Leu Trp Arg His Gly G1u Asp2885 2890 2895Leu Leu Ala Asp Ile Ala Leu Pro Met Ala Asp Asp Ser Thr Asp2900 2905 2910Thr Leu Val Leu His Pro Ala Leu Leu Asp Ser Ala Leu His Pro2915 2920 2925Leu Leu Ala Val Met Asp Thr Ser Gly Asp Gln Val Trp Leu Pro2930 2935 2940Phe Ser Trp Ser Gly Val Thr Leu His Ala Thr Gly Ala Thr His2945 2950 2955Ala Arg Val Arg Val Thr Pro His Asp Asp His Glu His Arg Ile2960 2965 2970Ala Leu Thr Asp Thr Ala Gly Arg Pro Ile Leu Thr Ala Asn Ala2975 2980 2985Val Ala Val Arg Pro Thr Arg Leu Glu Ala Pro Gln Gln Pro Leu2990 2995 3000Ser Glu Gly Leu Phe Ser Leu Glu Trp Thr Pro Val Ser Thr Leu3005 3010 3015Ala Asp Arg Ser Asp Ala Ala Ala Pro Thr Pro Gly Val Val Leu3020 3025 3030Ala Lys Ala Pro Val Ala Glu Gly Glu Gly Gly Glu Leu Glu Ala3035 3040 3045Val Gln Arg Ala Leu Thr Leu Val Gln Asp Trp Leu Ala Glu Pro3050 3055 3060Arg Pro Asp Asp Ala Arg Leu Val Val Met Thr Arg Asp Ala Val3065 3070 3075Ala Val Asp Gly Glu Ala His Ile Asp Pro Val Ala Ala Ala Val3080 3085 3090Trp Gly Leu Ile Arg Ser Ala Gln Thr Glu Asn Pro Gly Arg Phe3095 3100 3105Val Leu Leu Asp Arg Asp Leu Asp Thr Glu Leu Asp Thr Asp Pro3110 3115 3120Val Leu Gly Pro Asp Ala Leu Ala Glu Ala Asp Gly Arg Val Ala3125 3130 3135Glu Ala Val Arg Cys Ala Leu Asp Leu Asp Glu Ser Gln Val Ala3140 3145 3150Leu Arg Gly Gly Arg Val Leu Val Pro Arg Leu Val Arg Ala Thr3155 3160 3165Ala Ser Ala Thr Leu Pro Gly Pro Val Asp Arg Arg Asn Trp Arg3170 3175 3180Leu Glu Ala Ala Thr Pro Ala Gly Ala Ala Ser Leu Asp Ala Val3185 3190 3195Ala Pro Val Pro Phe Pro Glu Ala Glu Glu Glu Pro Ala Ala Gly3200 3205 3210Arg Val Arg Ile Glu Val Arg Ala Ala Gly Val Thr Phe Arg Asp3215 3220 3225Val Leu Ile Ala Thr Gly Gly Val Pro Asp Glu Thr Arg Leu Gly3230 3235 3240Gly Glu Gly Ala Gly Val Val Leu Glu Val Gly Pro Asp Val Thr3245 3250 3255Asp Val Ala Pro Gly Asp Arg Val Met Gly Val Phe Asp Gly Ala3260 3265 3270Phe Gly Arg Val Ala Asp Ala Asp Ala Arg Met Val Thr Arg Met3275 3280 3285Pro Arg Thr Trp Asp Phe Thr Arg Ala Ala Gly Val Pro Val Ala3290 3295 3300Phe Leu Thr Ala Trp Tyr Gly Leu Val Glu Leu Ala Asp Leu Arg3305 3310 3315Ala Gly Glu Ser Val Leu Ile His Ala Ala Thr Gly Gly Val Gly3320 3325 3330Thr Ala Ala Val Gln Ile Ala Arg His Leu Gly Ala Asp Ala Tyr3335 3340 3345Ala Thr Ala Asp Pro Ala Glu His His Val Leu Glu Ala Met Gly
3350 3355 3360Ile Asp Glu Ala His Arg Ala Ser Ser Arg Asp Leu Asp Phe Glu3365 3370 3375Asn Ala Phe Arg Ala Ala Thr Gly Gly Arg Gly Val Asp Val Val3380 3385 3390Leu Asn Ser Leu Thr Gly Asp His Ile Asp Asp Arg Thr Asp Ala3395 3400 3405Ser Leu Arg Leu Leu Ala Glu Gly Gly Arg Phe Leu Asp Pro Gly3410 3415 3420Arg Ala Asp Ala Arg Asp Pro Glu Gln Leu Ala Lys Asp Phe Pro3425 3430 3435Ala Val Asp Tyr Arg Val Tyr Asp Leu Val Pro Asp Ala Gly Pro3440 3445 3450Glu Arg Val Gln Pro Met Leu Ala Ala Leu Val Ala Leu Phe Asp3455 3460 3465Glu Gly Val Leu Ala Pro Leu Pro Val Arg Ala Trp Pro Leu Ala3470 3475 3480Arg Ala Arg Gln Ala Leu Arg His Met Ser Arg Ala Glu His Thr3485 3490 3495Gly Lys Leu Val Leu Thr Val Pro Pro Ala Leu Asp Pro Asp Gly3500 3505 3510Thr Val Leu Ile Thr Gly Gly Thr Gly Val Leu Ala Gly Leu Val3515 3520 3525Ala Glu His Leu Val Thr Thr His His Ile Thr His Leu His Leu3530 3535 3540Leu Ser Arg Arg Gly Pro Asp Ala Pro Gly Ala Thr Asp Leu Ala3545 3550 3555Thr Arg Leu Ala Glu Leu Gly Ala Thr Val His Ile Thr Ala Ala3560 3565 3570Asp Ala Ser Asp Pro Gly Ala Leu Arg Arg Val Val Asp Ala Ile3575 3580 3585Asp Pro Asp His Pro Leu Thr Gly Val Val His Thr Ala Gly Ile3590 3595 3600Val Glu Asp Ala Val Val Thr Ser Gln Thr Pro Asp Thr Leu Arg3605 3610 3615Arg Val Trp Thr Ala Lys Ala Thr Ser Ala Ala Asn Leu His Gln3620 3625 3630Ala Thr Lys His Leu Pro Leu Ala Met Phe Thr Leu Tyr Ser Ser3635 3640 3645Val Ser Gly Thr Leu Gly Asn Pro Gly Gln Ala Asn Tyr Ala Ala3650 3655 3660Ala Asn Ala Tyr Cys Asp Ala Leu Ala Ala Gln Arg Gln His Ala3665 3670 3675Gly Leu Pro Ala Thr Ser Ile Ala Trp Gly Leu Trp Ser Thr Ala3680 3685 3690Ser Asp Ile Thr Gly Gln Leu Ser Gln Ala Asp Val Ala Arg Met3695 3700 3705Gly Arg Ala Gly Val Arg Ala Leu Ala Thr Glu His Ala Leu Ala3710 3715 3720Leu Phe Asp Ala Ala His Arg Gln Gly Asp Pro Gln Leu Val Ala3725 3730 3735Leu Asn Leu Asp Val Pro Ala Leu Ala Ala Gln Pro Val Ala Ile3740 3745 3750Leu Pro Ala Ala Leu Arg Gly Leu Ala Thr Arg Ser Gly Gly Thr3755 3760 3765Thr Arg Arg Ala Ala Ala Ala Val Gln Arg Pro Asp Asp Trp Thr3770 3775 3780Arg Arg Leu Ala Gly Leu Pro Glu Ala Glu Gln Arg Gln Gln Leu3785 3790 3795Leu Thr Leu Val Arg Gly Asn Ala Ala Thr Val Leu Gly His Ala3800 3805 3810Asp Ser Glu Arg Val Arg Glu Glu Ala Pro Phe Lys Asp Leu Gly3815 3820 3825Phe Asp Ser Leu Thr Gly Val Glu Leu Arg Asn Arg Leu Ser Ala3830 3835 3840Ala Thr Gly Leu Arg Leu Pro Ala Ala Leu Val Phe Asp Phe Pro3845 3850 3855Ser Ala Lys Ser Leu Ala Asp Tyr Leu Arg Gly Arg Leu Val Ala3860 3865 3870Asp Gly Gly Ser Ala Ala Gln Ala Gly Val Asp Pro Val Leu Gly3875 3880 3885Glu Leu Ala Arg Leu Glu Ser Thr Leu Ser Ala Leu Asp Leu Pro3890 3895 3900Glu Ala Asp Ala Arg Ala Val Thr Asp Arg Leu Glu Gly Leu Leu3905 3910 3915Ala Gln Trp Lys Ala Ala Ser Ala Pro Pro Ala Glu Asp Asn Ala3920 3925 3930Ala Asp Arg Leu Thr Leu Ala Thr Ala Asp Glu Val Leu Ala Phe3935 3940 3945Ile Asp Asn Glu Leu Gly Thr Ser3950 3955<210>13<211>3979<212>PRT<213>南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400>1Met Pro Asp Glu Glu Arg Leu Val Asp Tyr Leu Lys Arg Val Ala Thr1 5 10 15Asp Leu His Asp Thr Arg Arg Arg Leu Arg Glu Val Glu Glu Arg His20 25 30Gln Glu Pro Ile Ala Ile Thr Ala Met Thr Cys Arg Phe Pro Gly Gly35 40 45Val Asp Ser Pro Glu Ala Leu Trp Asp Leu Val Ala Ser Gly Gly Asp50 55 60Val Ile Gly Pro Phe Pro Ala Asp Arg Gly Trp Asp Leu Glu Gly Leu65 70 75 80Tyr His Pro Asp Pro Asp His Pro Gly Thr Thr Tyr Thr Arg Glu Gly85 90 95Gly Phe Leu Arg Asp Ala Asp Thr Phe Asp Ser Gly Phe Phe Glu Ile100 105 110Ser Pro Arg Glu Ala Leu Val Met Asp Pro Gln Gln Arg Lys Leu Leu115 120 125Glu Val Thr Trp Glu Leu Phe Glu Arg Ala Gly Leu Asp Ala Thr Ser130 135 140Leu Arg Gly Ser Arg Thr Gly Val Phe Ile Gly Ala Ala Thr Met Gly145 150 155 160Ser Gly Thr Pro Ser Gly Pro Ala Arg Lys Glu Ser Glu Gly Tyr Val165 170 175Gly Val Ala Pro Ser Met Leu Ser Gly Arg Leu Ser Tyr Thr Phe Gly
180 185 190Leu Glu Gly Pro Ser Leu Thr Val Glu Thr Ala Cys Ser Ala Ser Leu195 200 205Val Ala Met His Gln Gly Ile His Ala Leu Arg Gln Gly Glu Cys Gly210 215 220Leu Ala Val Val Gly Gly Val Thr Ile Met Ser Ser Pro Ala Val Phe225 230 235 240Ile Gly Phe Ala Arg Gln Arg Gly Leu Ala Pro Asn Gly Arg Cys Lys245 250 255Pro Phe Ala Ala Gly Ala Asp Gly Thr Gly Trp Gly Glu Gly Ala Gly260 265 270Leu Val Leu Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Gln275 280 285Val Leu Ala Val Ile Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser290 295 300Asn Gly Phe Ser Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg305 310 315 320Gln Ala Leu Leu Asn Ala Arg Leu Ser Ser Ala Glu Val Asp Ala Val325 330 335Glu Ala His Gly Thr Gly Thr Arg Leu Gly Asp Pro Ile Glu Ala Asp340 345 350Ala Leu His Ala Thr Tyr Gly Gln Arg Arg Pro Ala Asp Arg Pro Leu355 360 365Leu Leu Gly Ser Val Lys Ser Asn Ile Gly His Pro Gln Ala Ala Ala370 375 380Gly Val Ala Gly Val Ile Lys Thr Val Met Ala Ile Arg His Gly Leu385 390 395 400Phe Pro Ala Thr Leu His Ile Asp Glu Pro Thr Pro His Val Asp Trp405 410 415Gly Ser Gly Ala Ile Arg Leu Val Thr Glu Pro Val Glu Trp Pro Glu420 425 430Thr Asp His Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Val Ser Gly435 440 445Thr Asn Ala His Val Ile Ile Glu Gln Ala Pro Asp Pro Asp Thr Ser450 455 460Glu Thr Asp Ala Gly Glu Thr Asp Ala Gly Glu Thr Ala Arg Asp Ala465 470 475 480Glu Gly Ala Ala Pro Ala Arg Gln Ala Val Val Ala Gly Gly Val Val485 490 495Pro Trp Met Leu Ser Ala Arg Asp Glu Ala Ala Leu Ala Arg Gln Ala500 505 510Leu Arg Leu Ala Glu Val Ala Glu Gly Asp Pro Ala Ala Asp Val Thr515 520 525Asp Met Gly Trp Ser Leu Ala Thr Thr Arg Ala Arg Phe Glu His Arg530 535 540Ala Val Val Val Gly Thr Asp Arg Ala Thr Leu Leu Asp Gly Leu Ala545 550 555 560Lys Leu Ala Ala Asp Glu Pro Asp Pro Ala Val Val Thr Ala Thr Ala565 570 575Gly Pro Ile Gly Ala Gly Pro Val Phe Val Phe Pro Gly Gln Gly Ala580 585 590Gln Trp Pro Gly Met Ala Arg Glu Leu Leu Asp Ser Ser Pro Val Phe595 600 605Ala Ala Arg Ile Ala Glu Cys Glu Arg Ala Leu Gly Pro Tyr Val Asp610 615 620Trp Ser Leu Thr Glu Val Leu Arg Gly Thr Asp Pro Ala Thr Asp Pro625 630 635 640Gly Arg Asp Asp Val Ile Gln Pro Val Leu Trp Ala Ile His Val Ser645 650 655Leu Ala Ala Val Trp Gln Ser Phe Gly Ile Thr Pro Ala Ala Val Val660 665 670Gly His Ser Gln Gly Glu Val Ala Ala Ala Cys Val Ala Gly Ala Leu675 680 685Thr Leu Asp Asp Ala Ala Lys Val Ile Ala Leu Arg Val Gln Ala Leu690 695 700Arg Pro Leu Ile Gly His Gly Ala Met Ala Ser Leu Ser Leu Gly Ala705 710 715 720Glu Asp Thr Ala Arg Leu Leu Ala Glu Leu Gly Ala Ala Ala Gly Asp725 730 735Val Ala Val Ala Ala Val Asn Gly Pro His Ala Thr Val Val Ser Gly740 745 750Ser Pro Asp His Leu Asp Ala Val Leu Glu Thr Ala Arg Glu Arg Gly755 760 765Ala Arg Thr Arg Thr Ile Asp Val Glu Tyr Ala Ser His Gly Pro His770 775 780Val Asp Arg Ile Arg Asp Asp Ile Val Ser Ala Leu Arg Asp Val Thr785 790 795 800Pro Val Glu Ser Glu Ile Ala Phe Tyr Ser Thr Val Thr Ala Glu Arg805 810 815Leu Asn Thr Ala Glu Leu Gly Thr Glu Tyr Trp Phe Asp Asn Leu Arg820 825 830Arg Pro Val Arg Phe Ala Asp Ala Val Gly Arg Leu Leu Ala Asp Gly835 840 845Tyr Arg Ala Phe Ile Gln Cys Asn Pro His Pro Ile Leu Ser Thr Ser850 855 860Leu Gln Asp Ile Phe Glu Glu Ser Gly Thr Arg Ala Ala Ser Leu Ala865 870 875 880Thr Leu Arg Arg Asp His Gly Gly Ala His Gln Leu Ala Leu Ala Leu885 890 895Ala Gln Ala His Ala Ala Gly Val Glu Val Asp Trp Arg Pro Trp Phe900 905 910Pro Ala Asp Arg Thr Pro Arg Thr Val Glu Leu Pro Thr Tyr Pro Phe915 920 925Gln Gly Lys Arg Tyr Trp Ile Pro Val Gly Gly Ser Gly Ala Gly Asp930 935 940Val Ser Ala Ala Gly Leu Arg Ala Val Asp His Pro Leu Leu Ala Ala945 950 955 960Ala Val Ser Leu Pro Asp Gly Gly Met Val Leu Thr Gly Arg Leu Ser965 970 975Ala Thr Thr Gly Ala Gly Trp Leu Ala Asp His Val Val Gly Asp Thr980 985 990Thr Leu Leu Pro Gly Ala Ala Met Val Glu Trp Ala Leu Gln Ala Ala995 1000 1005His Glu Ala Gly Cys Ala Ala Val Glu Glu Leu Ala Leu Gln Thr1010 1015 1020Pro Phe Val Leu Pro Ala Ser Gly Ala Leu Arg Val Arg Val Ala1025 1030 1035Val Gly Pro Ala Asp Asp Glu Gly Arg Arg Thr Val Asp Val Tyr1040 1045 1050Ser Arg Pro Asp Glu Leu Asp Thr Glu Thr Pro Asp Gly Trp Val1055 1060 1065Cys His Ala Met Gly Val Leu Ala Pro Glu Ala Pro Glu Asp Arg1070 1075 1080Thr Ala Pro Pro Asp Ala Pro Ala Ala Pro Trp Pro Pro Arg Gly1085 1090 1095Ala Glu Pro Leu Asp Val Thr Asp Phe Tyr Glu Arg Ala Ala Ala1100 1105 1110Gly Gly Tyr Gly Tyr Gly Pro Ala Phe Arg Gly Leu Thr Ala Ala1115 1120 1125Trp Arg Asp Gly Ala Asp Leu Leu Ala Glu Ile Ala Leu Pro Glu1130 1135 1140Ala Ala Gly Glu Gly Ala Asp Arg Phe Gly Ile His Pro Ala Leu1145 1150 1155Leu Asp Ala Ala Thr His Pro Thr Ile Leu Gly Gly Gly Arg Glu1160 1165 1170Asp Gly Ser Asp Ala Gly Gln Val Trp Leu Pro Phe Ala Trp Ser1175 1180 1185Gly Val Ser Leu Trp Ala Thr Gly Ala Arg Arg Val Arg Val Arg1190 1195 1200Ile Phe Pro Glu Asp Asn Gly Gln Arg Ile Ser Leu Thr Asp Glu1205 1210 1215Thr Gly Ala Pro Val Leu Glu Ala Ala Ser Val Ala Ala Arg Pro1220 1225 1230Thr Gly Leu Ala Glu Leu Arg Ala Leu Gly Ala Arg Ala Ala Glu1235 1240 1245Gly Leu Phe Val Val Asp Trp Val Pro Ala Arg Gly Gly Thr Gly1250 1255 1260Asp Ala Pro Pro Pro Asp Asp Gly Gly Trp Ala Thr Val Gly Gly1265 1270 1275Gly Gly Val Arg Leu Ala Gly Val Ala Asp His Ala Asp Leu Gly1280 1285 1290Ala Leu Leu Ala Ala Val Asp Asp Gly Ala Pro Val Pro Thr Val1295 1300 1305Val Leu His Pro Val Pro Ala Thr Ala Thr Pro Asp Asp Gly Leu1310 1315 1320Ala Ala Val Gly Gly Val Leu Ala Leu Ile Arg Glu Trp Leu Ala1325 1330 1335Glu Pro Arg Trp Leu Asp Ser Arg Leu Val Leu Val Thr Ser Asp1340 1345 1350Ala Val Ser Ala Gly Asp Asp Glu Gly Ala Val Asp Pro Gly Gly1355 1360 1365Ala Ala Val Trp Gly Leu Val Arg Ser Val Gln Ala Glu His Pro1370 1375 1380Gly Arg Phe Thr Leu Leu Asp Val Gly Gly Asp Thr Asp Ala Asp1385 1390 1395Ala Gly Gly Gly Glu Ser Leu Ala Glu Ala Val Arg Arg Ser Ile1400 1405 1410Asp Ala Asp Glu Pro Gln Val Val Val Arg Ala Ala Gly Thr Leu1415 1420 1425Val Pro Arg Leu Val Arg Thr Ala Pro Ala Ala Glu Ala Asp Thr1430 1435 1440Pro Glu Leu Ser Gly Gly Thr Val Leu Val Ser Gly Gly Thr Gly1445 1450 1455Val Leu Gly Gly Ala Ala Ala Glu His Leu Val Arg Ala His Gly1460 1465 1470Val Glu Arg Val Leu Leu Leu Ser Arg Arg Gly Pro Asn Ala Pro1475 1480 1485Glu Ala Ala Glu Leu Val Arg Arg Leu Thr Ala Leu Gly Ala Gln1490 1495 1500Val Asp Val Ala Ala Val Asp Val Ala Asp Arg Ala Ala Leu Ala1505 1510 1515Glu Thr Leu Arg Thr Ile Pro Asp Ser His Pro Leu Leu Gly Val
1520 1525 1530Val His Ser Ala Gly Val Thr Asp Asp Ala Leu Val Glu Ser Trp1535 1540 1545Asp Ala Asp Arg Leu Thr Arg Val Trp Glu Pro Lys Ala Thr Gly1550 1555 1560Ala Trp His Leu His Thr Leu Thr Arg Asp Leu Pro Leu Arg Met1565 1570 1575Phe Val Val Phe Ser Ser Ala Ala Gly Val Val Gly Asn Ser Gly1580 1585 1590Gln Ala Gly Tyr Ala Ala Ala Asn Ala Cys Thr Asp Ala Leu Ile1595 1600 1605Ala His Arg Arg Ala Ala Gly Leu Pro Gly Thr Ser Val Ala Trp1610 1615 1620Thr Leu Trp Glu Gln Ala Ser Ala Met Thr Glu His Leu Thr Glu1625 1630 1635Ala Asp Leu Ser Arg Leu Gly Thr Leu Gly Met Arg Pro Leu Ala1640 1645 1650Thr Ser Arg Ala Leu Gly Leu Leu Asp Ala Ala Leu His Val Thr1655 1660 1665His Pro Val Val Val Ala Ala Asp Leu Asp Ala Thr Arg Leu Gly1670 1675 1680Pro Asp Ser Pro Ala Met Leu Arg Ala Leu Ala Arg Pro Ala Arg1685 1690 1695Arg Arg Ala Met Glu His His Ala Thr Gly Pro Ala Leu Ala Gly1700 1705 1710Arg Leu Ala Gly Leu Asp Ala Thr Ala Arg Arg Asp Leu Leu Leu1715 1720 1725Gln Thr Val Arg Gln Met Val Thr Val Val Leu Gly His Ser Ser1730 1735 1740Asp Ala Ala Ile Arg Ala Glu Ala Ala Phe Lys Glu Leu Gly Phe1745 1750 1755Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Ala Gly Ala1760 1765 1770Thr Gly Leu Arg Leu Pro Ala Thr Leu Val Phe Asp Tyr Pro Thr1775 1780 1785Pro Leu Ala Leu Ala Asp His Leu Leu Glu Arg Leu Thr Ala Thr1790 1795 1800Ala Ser Pro Ala Ser Pro Arg Ala Val Pro Ser Arg Ala Gly Ala1805 1810 1815Ala Asp Glu Pro Ile Ala Val Val Ser Met Ala Cys Arg Phe Pro1820 1825 1830Gly Gly Val Thr Thr Pro Glu Glu Leu Trp Asp Leu Val Ala Ala1835 1840 1845Asp Arg His Val Leu Gly Pro Phe Pro Thr Asn Arg Gly Trp Asp1850 1855 1860Leu Ala Asn Leu Phe His Pro Asp Pro Asp His Pro Gly Thr Thr1865 1870 1875Tyr Ala Ser Glu Gly Ala Phe Met Tyr Asp Ala Asp Gly Phe Asp1880 1885 1890Ala Ala Phe Phe Gly Ile Asn Pro Arg Glu Ala Leu Ala Met Asp1895 1900 1905Pro Gln Gln Arg Val Leu Leu Glu Thr Ser Trp Glu Leu Leu Glu1910 1915 1920Arg Ala Gly Ile Asp Pro His Thr Leu Lys Asp Ser Leu Thr Gly1925 1930 1935Val Tyr Ala Gly Val Met Tyr His Asp Tyr Gly Asn Gly Leu Pro1940 1945 1950Pro Gly Asp Pro Arg Leu Asp Gly Tyr Ala Gly Leu Ser Gly Thr1955 1960 1965Ser Ser Ile Ile Ala Gly Arg Val Ala Tyr Thr Leu Gly Leu Gln1970 1975 1980Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val1985 1990 1995Thr Met His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu Cys Asp2000 2005 2010Leu Ala Leu Ala Gly Gly Val Thr Val Leu Ala Thr Pro Asp Val2015 2020 2025Phe Thr Gly Phe Ser Arg Gln Arg Gly Leu Ala Pro Asp Gly Arg2030 2035 2040Cys Lys Pro Phe Ala Ala Ala Ala Asp Gly Thr Gly Phe Gly Glu2045 2050 2055Gly Val Gly Leu Val Leu Leu Glu Arg Leu Ser Asp Ala His Arg2060 2065 2070Asn Gly His Arg Val Leu Ala Val Leu Arg Gly Ser Ala Val Asn2075 2080 2085Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ala2090 2095 2100Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Gly Ala Glu Leu Asp2105 2110 2115Pro Ala Asp Val Asp Ala Val Glu Ala His Gly Thr Gly Thr Thr2120 2125 2130Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly2135 2140 2145Gln Asp Arg Pro Thr Asp Arg Pro Leu Trp Leu Gly Ser Ile Lys2150 2155 2160Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Val
2165 2170 2175Ile Lys Met Ile Met Ala Met Asp His Gly Arg Leu Pro Thr Ser2180 2185 2190Leu His Ile Asp Glu Pro Ser Pro His Ile Asp Trp Thr Thr Gly2195 2200 2205Asn Val Arg Leu Leu Thr Glu Pro Ala Asp Trp Pro Ala Thr Asp2210 2215 2220Arg Pro Arg Arg Ala Ala Val Ser Ser Phe Gly Ala Ser Gly Thr2225 2230 2235Asn Ala His Leu Ile Leu Glu Gln Ala Pro Asp Arg Pro Gly Asp2240 2245 2250Gly Pro Ala Gly Asp Arg Pro Ala Pro Val Ala Val Ala Trp Pro2255 2260 2265Leu Ser Ala Arg Thr Asp Glu Ala Leu Arg Thr Val Ala Thr Ala2270 2275 2280Leu Ala Asp Arg Leu Gly Ala Asp Asp Thr Thr Pro Val Thr Asp2285 2290 2295Val Gly Trp Ser Leu Ala Thr Ala Arg Ala Thr Phe Glu Arg Arg2300 2305 2310Ala Val Ile Ile Gly Ser Asp Arg Gln Glu Met Thr Ala Ala Leu2315 2320 2325Asp Ala Leu Ala Arg Asp Leu Pro His Pro Asn Leu Val Ala Pro2330 2335 2340Leu Pro Val Ala Pro Pro Ala Gly Asp Thr Val Trp Leu Phe Ser2345 2350 2355Gly Gln Gly Ser Gln Arg Pro Gly Met Gly Ala Glu Leu His Glu2360 2365 2370Arg Phe Pro Ala Phe Ala Asp Thr Phe Asp Glu Ile Cys Ala Leu2375 2380 2385Ile Asp Pro His Leu Asp His Pro Leu Arg Asp Ile Val Phe Ala2390 2395 2400Thr His Pro Asp His Thr Asp Leu Leu Asn His Thr Thr Tyr Thr2405 2410 2415Gln Ala Gly Leu Phe Ala Val Gln Val Ala Leu Ala Arg Leu Leu2420 2425 2430Glu His Cys Gly Leu Arg Pro Asp Thr Val Ile Gly His Ser Ile2435 2440 2445Gly Glu Ile Thr Ala Ala His Ile Ala Gly Val Leu Ser Leu Gln2450 2455 2460Asp Ala Cys His Leu Val Ala Asn Arg Ala Thr Leu Leu Gly Lys2465 2470 2475Leu Pro Pro Gly Gly Ala Met Thr Ala Ile Glu Ala Thr Ala Glu2480 2485 2490Glu Ile Thr Gln Thr Leu Thr Pro Tyr His Gly Gln Val Thr Ile2495 2500 2505Ala Ala Leu Asn Ala Pro Thr Ser Thr Val Ile Ser Gly Pro Glu2510 2515 2520Glu Leu Val Ala Gln Leu Thr Arg Arg Trp Lys Glu Arg Gly Arg2525 2530 2535Arg Thr Lys Thr Leu Thr Val Ser His Ala Phe His Ser Pro Leu2540 2545 2550Met Glu Pro Ala Leu Asn Asp Phe Arg His Ala Ile Asp His Leu2555 2560 2565Thr Tyr His Gln Pro Thr Ile Pro Leu Ile Ser Asn Leu Thr Gly2570 2575 2580Glu Pro Ala Thr Gln Asp Ile Ala Thr Pro Asp Tyr Trp Val Arg2585 2590 2595His Ile Arg Gln Pro Val His Phe His Pro Ala Ile Thr His Ile2600 2605 2610Ala Pro His Thr Ala Ala Phe Leu Glu Ile Gly Pro Asp Ala Thr2615 2620 2625Leu Ile Pro Ala Thr Gln Asn Thr Leu Asp Thr Leu Glu Asn Gln2630 2635 2640Pro Thr Ser Ala Pro Gln Leu Ile Pro Thr Leu Thr Arg Lys Gln2645 2650 2655Pro Asp Thr Gln Ala Leu Ala His Ala Leu Ala Arg Leu His Thr2660 2665 2670Leu Thr Pro Leu Asn Trp His Pro Trp Tyr Thr Asp Gln Pro Thr2675 2680 2685Pro Thr Thr Ile Asp Leu Pro Thr Tyr Pro Phe Gln His Glu Arg2690 2695 2700Tyr Trp Leu Thr Pro Thr His Ala Gly Pro Thr Thr Pro Gly Ala2705 2710 2715Thr Pro Leu Thr His Pro Phe Leu Ala Ala Thr Ala Pro Leu Ala2720 2725 2730Asp Gly Gly Leu Leu Leu Thr Gly Gln Val Pro Ser Ala Asp His2735 2740 2745Ala Gly Trp His Thr Glu His Thr Ile Ala Gly Ala Thr Leu Leu2750 2755 2760Pro Ala Thr Ala Leu Leu Glu Ile Ala Leu His Ala Ala Asp His2765 2770 2775Thr Thr Thr Pro His Ile Asp Glu Leu Ile Leu Gln His Pro Leu2780 2785 2790Thr Leu Asp Pro Ser His Pro Leu Ala Leu Gln Ala Ile Val Ser2795 2800 2805Pro Ala Asp Asp Ser Gly His Arg Ala Leu His Ile Tyr Thr Arg
2810 2815 2820Ala Pro Ser Ser Pro Thr Ala Glu Trp Thr His His Ala Thr Ala2825 2830 2835Thr Leu Gly Gly Glu Pro Thr Ala Glu Arg Pro Thr Thr Glu Ala2840 2845 2850Glu Ala Ala Trp Pro Pro Pro Gly Ala Lys Ala Val Asp Ile Thr2855 2860 2865Gly Phe Tyr Asp Arg Ala Ala Ala Asp Gly Tyr His Tyr Gly Pro2870 2875 2880Ser Tyr Gln Gly Leu Gln Thr Val Trp Arg Gln Gly Glu Asp Leu2885 2890 2895Leu Ala Asp Ile Thr Leu Pro Thr Ala Gly Thr Pro Asp His Thr2900 2905 2910Thr Asp Ser Leu Ala Ile His Pro Ala Leu Leu Asp Ala Ala Leu2915 2920 2925His Pro Leu Leu Ala Thr Ala Asp Asn Pro Asp Gly Glu Ile Trp2930 2935 2940Leu Pro Phe Thr Trp Ser Gly Val Thr Leu His Ala Thr Gly Ala2945 2950 2955Thr His Val Arg Ala Arg Ile Thr Pro Gln Gly Asp Asn Asp Tyr2960 2965 2970Arg Leu Thr Leu Thr Asp Ala Thr Gly Gln Thr Val Leu Thr Ala2975 2980 2985Gly Thr Ile Ala Ser Arg Pro Leu Asp Thr Ala Arg Leu Arg Thr2990 2995 3000Arg Gly Pro Gly Asp Gly Leu Tyr Gln Val Arg Trp Thr Ala Met3005 3010 3015Pro Ile Pro Ala Gly Ser Ala Thr Ala Val Ala Asp Asp Trp Ala3020 3025 3030Met Leu Gly Asp Ala Gly Leu Arg Asp Gly Gly Leu Ala Asp Ala3035 3040 3045Val Ala Pro Leu Ala Ser Tyr Pro Asp Val Ala Ala Leu Val Ala3050 3055 3060Ala Met Asp Asp Gly Thr Pro Val Pro Ser Val Val Leu Thr Gly3065 3070 3075Leu Ala Pro Ala Asp Gly Gly Asp Ala Asp Val Val Val Glu Val3080 3085 3090Leu Thr Thr Ala Arg Glu Trp Leu Ala Glu Pro Arg Leu Ala Glu3095 3100 3105Ser Arg Leu Val Val Val Thr His Asp Ala Ala Val Ala Glu Asp3110 3115 3120Thr Asp Ser Gly Pro Asp Gly Gly Asp Val Asp Pro Val Ala Ala3125 3130 3135Gly Val Trp Gly Leu Ile Arg Ser Ala Gln Ser Glu Asn Pro Gly3140 3145 3150Arg Phe Thr Leu Leu Asp Leu Thr Arg Arg Asp Ala Gly Thr Ala3155 3160 3165Pro Asp Val Val Glu Val Leu Arg Ala Ala Met Asp Ala Asp Glu3170 3175 3180Trp Gln Val Ala Val Arg Gly Gly Arg Ala Leu Val Pro Arg Leu3185 3190 3195Thr Ala Ala Asp Ala Ala Ala Gly Ile Val Leu Pro Val Gly Ala3200 3205 3210Pro Ala Trp Gln Leu Val Met Ala Asp Glu Arg Ala Gly Thr Val3215 3220 3225Asp Gly Leu Ala Pro Glu Glu Cys Pro Glu Val Leu Glu Pro Leu3230 3235 3240Ala Pro Gly Gln Val Arg Ile Ala Val Arg Ala Ala Gly Val Asn3245 3250 3255Phe Arg Asp Val Met Val Thr Leu Gly Val Val Pro Asp Arg Arg3260 3265 3270Gly Leu Gly Gly Glu Gly Ala Gly Met Val Leu Asp Val Ala Pro3275 3280 3285Asp Val Thr Ser Val Ala Val Gly Asp Arg Val Met Gly Leu Phe3290 3295 3300Gln Gly Ser Phe Gly Pro Ile Ala Val Ala Asp Ala Arg Ala Leu3305 3310 3315Val Pro Val Pro Pro Gly Trp Thr Asp Arg Gln Ala Ala Ala Val3320 3325 3330Pro Ile Ala Phe Leu Thr Ala Trp Tyr Gly Leu Ile Asp Leu Ala3335 3340 3345Gly Leu Lys Ala Gly Glu Ser Val Leu Ile His Ala Ala Thr Gly3350 3355 3360Gly Val Gly Thr Ala Ala Val Gln Ile Ala Arg His Leu Gly Ala3365 3370 3375Val Ile Tyr Ala Thr Ala Ser Pro Gly Lys His Pro Met Leu Glu3380 3385 3390Ala Met Gly Val Asp Glu Thr His Arg Ala Ser Ser Arg Asp Leu3395 3400 3405Asp Phe Glu His Ile Phe Arg Ala Ala Thr Gly Ser Glu Gly Met3410 3415 3420Asp Val Val Leu Asp Cys Leu Ala Gly Glu Phe Val Asp Ala Ser3425 3430 3435Leu Arg Leu Leu Gly Gln Gly Gly Arg Phe Ile Glu Met Gly Lys3440 3445 3450Thr Asp Ile Arg Asp Pro Glu Gln Ile Ala Asp Thr His Pro Gly
3455 3460 3465Val His Tyr Arg Ser Tyr Asp Leu Val Ser Asp Ala Gly Leu Asp3470 3475 3480Arg Leu Ser Glu Met Leu Gly Thr Leu Ala Asp Leu Phe Ala Gln3485 3490 3495Gly Val Leu Thr Pro Pro Pro Val Gln Ala Trp Pro Leu Ala Arg3500 3505 3510Ala Arg Gln Ala Leu Arg His Met Ser Gln Ala Lys His Thr Gly3515 3520 3525Lys Leu Val Leu Asp Ile Pro Pro Ala Leu Asp Pro Asp Gly Thr3530 3535 3540Val Leu Ile Thr Gly Gly Thr Gly Thr Leu Gly Ala Leu Ile Ala3545 3550 3555Glu His Leu Val Thr Asn His His Ile Thr His Leu His Leu Leu3560 3565 3570Ser Arg Arg Gly Pro Asp Ala Pro Gly Ala Ala Glu Leu Thr Ala3575 3580 3585His Leu Thr Glu Leu Gly Ala Thr Val His Ile Thr Ala Thr Asp3590 3595 3600Thr Thr Asp Pro His Ala Leu Arg Gln Ala Leu Asp Thr Val Asp3605 3610 3615Pro Arg His Pro Leu Thr Ala Val Ile His Thr Ala Gly Ile Val3620 3625 3630Asp Asp Ala Val Ile Thr Ala Gln Thr Ala Asp Ser Leu His Arg3635 3640 3645Val Trp Ala Ala Lys Ala Thr Ser Ala Ala Asn Leu His Gln Ala3650 3655 3660Thr Glu His Leu Pro Leu Ala Met Phe Val Ile Phe Ser Ser Ala3665 3670 3675Ala Gly Thr Phe Gly Ser Pro Gly Gln Ala Asn Tyr Ala Ala Ala3680 3685 3690Asn Ala Tyr Cys Asp Ala Leu Ala Thr Arg Arg Arg His Ala Gly3695 3700 3705Leu Pro Ala Thr Ser Ile Ala Trp Gly Leu Trp Ala Ala Thr Ser3710 3715 3720Gly Met Thr Gly Gly Leu Thr Glu Ile Asp His Ala Arg Met Ser3725 3730 3735Arg Ser Gly Met Ala Pro Leu Pro Ser Glu His Ala Leu Ala Leu3740 3745 3750Phe Asp Ala Ala His Gly Leu Gly Ala Ala Arg Val Leu Ala Ala3755 3760 3765Arg Leu Asp Leu Ala Arg Leu Ser Ala Gln Pro Thr Glu Ala Leu3770 3775 3780Pro Pro Leu Val Arg Ser Leu Thr Gly Thr Gly Pro Arg Thr Ala3785 3790 3795Arg Arg Ser Ala Ala Ala Pro Val Ala Asp Leu Ser Gly Arg Leu3800 3805 3810Ala Ser Met Ala Pro Ala Gly Gln Leu Ala Leu Leu Leu Asp Leu3815 3820 3825Val Arg Thr His Ala Ala Thr Val Leu Gly His Met Asp Ser Gly3830 3835 3840Thr Val Ser Ala Asp Thr Pro Phe Lys Asp Leu Gly Phe Asp Ser3845 3850 3855Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Thr Thr Val Thr Gly3860 3865 3870Leu Arg Leu Ser Ala Ala Ser Val Phe Arg Tyr Pro Thr Ala Thr3875 3880 3885Ala Met Ala Glu His Leu Arg Gly Glu Leu Cys Pro Thr Gly Asp3890 3895 3900Asp Thr Ala Gln Pro Val Leu Arg Glu Leu Ala Arg Leu Glu Ala3905 3910 3915Ala Val Gly Glu Ser Lys Pro Glu Gly Glu Thr Ser Ala Gln Leu3920 3925 3930Val Lys Arg Leu Gln Thr Leu Leu Trp Arg Leu Gly Asp Glu Ala3935 3940 3945Ala Ala Val Asp His Thr Val Asp Gly Glu Glu Leu Glu Ser Ala3950 3955 3960Ser Asp Asp Glu Met Phe Ala Leu Ile Asp Gln Gln Leu Gly Ser3965 3970 3975Ser<210> 14<211> 1665<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Val Ser Thr Thr Glu Glu Lys Leu Arg Gln Tyr Leu Lys Arg Val Thr1 5 10 15Leu Asp Leu Gly Gln Ala Lys Gln Arg Leu Arg Glu Ala Glu Glu Arg20 25 30His Gln Glu Pro Ile Ala Ile Thr Ala Met Ala Cys Arg Tyr Pro Gly35 40 45Gly Val Arg Ser Pro Glu Ala Leu Trp Asp Leu Val Ala Thr Arg Thr50 55 60Asp Ala Ile Gly Pro Phe Pro Thr Asn Arg Gly Trp Asp Leu Glu Gly65 70 75 80Leu Phe His Pro Asp Pro Asp His Tyr Gly Thr Ser Tyr Val Arg Glu85 90 95Gly Gly Phe Leu His Asp Ala Glu Arg Phe Asp Ala Ser Phe Phe Asn100 105 110Ile Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Val Leu115 120 125Leu Glu Thr Ala Trp Glu Leu Leu Glu Arg Ala His Ile Asp Pro His130 135 140Ser Leu Lys Gly Thr Leu Thr Gly Val Tyr Thr Gly Val Ser Ser Gln145 150 155 160Asp Tyr Leu Ser Arg Ile Pro Arg Ile Pro Glu Gly Phe Glu Gly Tyr165 170 175Thr Ala Thr Gly Gly Leu Met Ser Val Val Ser Gly Arg Val Ala Tyr180 185 190Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Leu Asp Thr Ala Cys Ser195 200 205Ala Ser Leu Val Ala Met His Leu Ala Gly Gln Ala Leu Arg Gln Gly210 215 220Glu Cys Asp Leu Ala Leu Ala Gly Gly Val Thr Val Phe Ser Thr Pro225 230 235 240Thr Ala Tyr Val Glu Phe Ser Arg Gln Arg Gly Phe Ala Pro Asp Ala245 250 255Arg Cys Lys Pro Phe Ala Ala Ala Ala Asp Gly Thr Gly Phe Ser Glu260 265 270Gly Val Gly Leu Val Leu Leu Glu Arg Leu Ser Asp Ala Gln Arg His275 280 285Gly Arg Arg Val Leu Ala Val Leu Arg Gly Ser Ala Val Asn Gln Asp290 295 300Gly Ala Ser Asn Gly Leu Ser Ala Pro Asn Asp Ala Ala Gln Glu Arg305 310 315 320Val Ile Arg Gln Ala Leu Asp Ser Ala Arg Leu Thr Ala Asp Gln Val325 330 335Asp Ala Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile340 345 350Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Lys Glu Arg Ser Ala Asp355 360 365Arg Pro Leu Trp Leu Gly Ser Val Lys Ser Asn Ile Gly His Thr His370 375 380Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val Met Ala Met His385 390 395 400His Gly Arg Leu Pro Ala Thr Leu His Val Asp Glu Pro Thr Ser His405 410 415Val Asp Trp Asp Thr Gly Thr Val Arg Leu Leu Thr Glu Pro Val Asp420 425 430Trp Pro Arg Gly Asp Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly
435 440 445Ile Ser Gly Thr Asn Ala His Val Ile Leu Glu Glu Ala Ala Leu Pro450 455 460Pro Ala Ala Thr Gly Ala Glu Arg Pro Gly Asp Arg Leu Thr Pro Trp465 470 475 480Val Val Ser Ala Arg Gly Gln Ala Ala Leu His Asp Gln Ala Arg Arg485 490 495Leu Leu Asp Ala Thr Val Asp Gly Asp Pro Glu Ala Val Gly Trp Ser500 505 510Leu Val Ala Ser Arg Ala Val Phe Asp Gln Arg Ala Val Ile Thr Gly515 520 525Arg Asp Thr Glu Thr Leu Arg Ala Gly Leu Ala Ala Leu Ala Ala Gly530 535 540Glu Asp His Pro Ala Leu Val Arg Arg Glu Ala Gly Val Pro Ala Ser545 550 555 560Gly Ser Gln Val Trp Leu Phe Ser Gly Gln Gly Ser Gln Arg Pro Gly565 570 575Met Gly Ala Glu Leu His Glu Arg Phe Pro Ala Phe Ala Asp Thr Phe580 585 590Asp Glu Ile Cys Ala Leu Ile Asp Pro His Leu Asp His Pro Leu Arg595 600 605Asp Ile Val Phe Ala Thr His Pro Asp His Thr Asp Leu Leu Asn His610 615 620Thr Thr Tyr Thr Gln Ala Gly Leu Phe Ala Val Gln Val Ala Leu Ala625 630 635 640Arg Leu Leu Glu His Cys Gly Leu Arg Pro Asp Thr Val Ile Gly His645 650 655Ser Ile Gly Glu Ile Thr Ala Ala His Ile Ala Gly Val Leu Ser Leu660 665 670Gln Asp Ala Cys His Leu Val Ala Asn Arg Ala Thr Leu Leu Gly Lys675 680 685Leu Pro Pro Gly Gly Ala Met Thr Ala Ile Glu Ala Thr Ala Glu Glu690 695 700Ile Thr Gln Thr Leu Thr Pro Tyr His Gly Gln Val Thr Ile Ala Ala705 710 715 720Leu Asn Ala Pro Thr Ser Thr Val Ile Ser Gly Pro Glu Glu Leu Val725 730 735Ala Gln Leu Thr Arg Arg Trp Lys Glu Arg Gly Arg Arg Thr Lys Thr740 745 750Leu Thr Val Ser His Ala Phe His Ser Pro Leu Met Glu Pro Ala Leu755 760 765Asn Asp Phe Arg His Ala Ile Asp His Leu Thr Tyr His Gln Pro Thr770 775 780Ile Pro Leu Ile Ser Asn Leu Thr Gly Glu Pro Ala Thr Gln Asp Ile785 790 795 800Ala Thr Pro Asp Tyr Trp Val Arg His Ile Arg Gln Pro Val His Phe805 810 815His Pro Ala Ile Thr His Ile Ala Pro His Thr Ala Val Phe Leu Glu820 825 830Ile Gly Pro Asp Ala Thr Leu Ile Pro Ala Thr Gln Asn Thr Leu Asp835 840 845Thr Leu Asp Lys Gln Pro Ala His Pro Pro Gln Leu Ile Pro Thr Leu850 855 860Thr Arg Lys Gln Pro Asp Thr Gln Ala Leu Ala His Ala Leu Ala Arg865 870 875 880Leu His Thr Leu Thr Pro Leu Asn Trp His Pro Trp Tyr Thr Asp Gln885 890 895Pro Thr Pro Thr Thr Ile Asp Leu Pro Thr Tyr Pro Phe Gln Arg Glu900 905 910Arg Tyr Trp Leu Pro Asp Ala Leu Ala Asp Ala Pro Pro Pro Glu Ala915 920 925Asp Glu Glu Gln Val Arg Phe Trp Asn Ala Val Glu Ala Gln Asp Leu930 935 940Pro Ala Leu Ser Asp Thr Leu Gly Ile Gly Glu Glu Asp Gly Arg Arg945 950 955 960Ser Ser Leu Gly Ala Val Leu Pro Thr Leu Ser Arg Trp His Gln Glu965 970 975Arg His Glu Arg Ala Thr Val Ser Ser Trp Arg Tyr Arg Val Gly Trp980 985 990Arg His Leu Pro Asp Leu Gly Pro Ala Ala Val Ala Gly Pro Trp Leu995 1000 1005Leu Val Val Pro Pro Lys Gly Ala Asp Ala Trp Ala Asp Ala Cys1010 1015 1020Glu Arg Ala Leu Thr Ala Asp Gly Gly Glu Val Arg Arg Leu Val1025 1030 1035Thr Asp Gly Arg Ala Asp Val Ala Glu Leu Ala Ala Ser Leu Arg1040 1045 1050Ala Leu Tyr Ala Glu Gly Pro Ser Pro Ala Gly Val Leu Ser Leu1055 1060 1065Leu Pro Leu Asp Glu Arg Pro His Glu Ala Phe Pro Ala Val Thr1070 1075 1080Gly Gly Val Thr Gly Thr His Val Leu Leu Arg Ala Leu Leu Asp1085 1090 1095Ala Glu Leu Asp Ala Pro Leu Trp Cys Ala Thr Arg Gly Ala Val1100 1105 1110Ala Val Asp Asp Asp Glu Ala Pro Glu Ala Pro Ala Gln Ala Gln1115 1120 1125Val Trp Gly Leu Gly Arg Val Ala Ala Leu Glu His Pro Thr Ala1130 1135 1140Trp Gly Gly Leu Val Asp Leu Pro Ala Ser Val Ala Asp Leu Ala1145 1150 1155Pro Asp Leu Leu Cys Ala Val Leu Ala Gly Arg Asn Gly Glu Asp1160 1165 1170Gln Val Ala Leu Arg Pro Ala Gly Ala Phe Gly Arg Arg Leu Leu1175 1180 1185Pro Ala Pro Leu Asp Ala Gln Ala Pro Ala Gln Glu Arg Ala Trp1190 1195 1200Thr Pro Arg Asp Gly Val Leu Val Thr Gly Gly Val Ala Gly Ala1205 1210 1215Ala Ala Leu Val Ala Arg Trp Leu Ala Ala Asp Gly Thr Lys His1220 1225 1230Ile Val Leu Leu Ala Pro Asp Gly Pro Ala Ala Pro Gly Gly Ala1235 1240 1245Glu Leu Val Ala Glu Leu Ala Glu Leu Gly Ala Glu Ala Thr Val1250 1255 1260Val Asp Gly Val Pro Ser Glu Pro Thr Thr Arg Gln Glu Leu Ala1265 1270 1275Asp Arg Leu Ala Ala Ser Gly Leu Arg Val Arg Thr Val Val His1280 1285 1290Ala Gly Ala Pro Gly Asp Trp Ala Pro Leu Ala Glu Leu Thr Pro1295 1300 1305Asp Glu Leu Ala Glu Ala Leu Ser Asp Ala Met Gly Gly Ala Asp1310 1315 1320Arg Leu Ala Glu Leu Cys Gly Leu Glu Pro Asp Asp Pro Val Val1325 1330 1335Val Phe Ser Ser Ile Ala Ala Val Trp Gly Gly Gly Gly His Gly1340 1345 1350Ala Arg Ala Ala Ala Asp Ala Tyr Leu Asp Ala Trp Ala Arg Arg1355 1360 1365Arg Gln Ala Ala Gly Gly His Val Ala Arg Leu Ala Trp Gly Val1370 1375 1380Trp Asp Gly Ser Glu Asp Pro Glu Ala Ala Glu Arg Ala Glu Arg1385 1390 1395Gln Gly Leu Leu Ala Leu His Pro Thr Pro Ala Leu Ala Ala Leu1400 1405 1410Arg Arg Thr Leu Asp His Ser Gly Asp Gly Thr Asp Gln Gly Thr1415 1420 1425Val Arg Ala Gly Asp Glu Gly Gly Asp Arg Ser Asp Val His Ala1430 1435 1440Val Ile Ala Asp Val His Trp Asp Arg Phe Val Pro Leu Phe Thr1445 1450 1455Met Ala Arg Ala Ser Arg Leu Phe Asp Glu Ile Pro Ala Ala Arg1460 1465 1470Arg Ala Trp Gln Ala Ala Leu Asp Ser Ser Asp Asp Glu Ser Ser1475 1480 1485Glu Ser Leu Thr Ala Leu Arg Asp Arg Leu Ala Ala Gln Ser Pro1490 1495 1500Gln Ala Arg Thr Gly Thr Leu Leu Ala Leu Val Arg Ala His Val1505 1510 1515Ala Gly Ala Leu Arg Tyr Pro Ala Ala Glu Ser Val Asp Pro Glu1520 1525 1530Gln Pro Phe Lys Glu Leu Gly Phe Asp Ser Leu Ala Ala Val Glu1535 1540 1545Phe Arg Asn Arg Leu Arg Gly Ala Ile Gly Leu Thr Leu Pro Ala1550 1555 1560Thr Leu Val Phe Asp Tyr Pro Thr Pro Thr Ala Leu Ala Gly Tyr1565 1570 1575Leu Val Ser Gln Val Leu Pro Ala Glu Pro Ala Asp Glu Pro Ala1580 1585 1590Ala Ala His Leu Asp Glu Ile Glu Ala Thr Leu Ala Ala Leu Asp1595 1600 1605Ala Asp Asp Pro Arg Arg Ala Gly Leu Thr His Arg Leu Arg Leu1610 1615 1620Leu Leu Trp Arg Tyr Ala Asp Gly Asp Asp Ala Leu Glu Pro Arg1625 1630 1635Glu Glu Thr Gly Gly Asp Asp Leu Glu Thr Ala Ser Ala Asp Glu1640 1645 1650Met Phe Ala Leu Ile Asp Arg Glu Phe Gly Glu Ser1655 1660 1665<210> 15<211> 460<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Val Arg Ser Met Thr Lys Val His Gly Arg Glu Ser Glu Leu Val Ala1 5 10 15Ile Ala Gly Asp Gln Arg Gly Glu Met Pro Ala Met Arg Val Leu Phe20 25 30Val Thr Ile Pro Trp Arg Thr His Phe Gln Phe Cys Val Pro Met Ala35 40 45Trp Ala Leu Arg Thr Ala Gly His Glu Val His Val Ala Ser Gly Pro50 55 60Asp Leu Thr Asp Val Ile Val Gln Ser Gly Leu Thr Ala Val Pro Val65 70 75 80Gly Ser Glu Glu His Phe Leu Glu Lys Ala His Gln Ala Gln Thr Glu85 90 95Ala Ser Glu Ala Thr Trp Gly Gly Ile His His Pro Ile Asp Leu Gly100 105 110Glu Asn Arg Glu Glu Met Phe Pro Leu Ser Tyr Leu Lys Ser Leu Cys115 120 125Ala Thr Ser Thr Glu Val Ala Lys Ala Val Asn Asp Ser Met Ile Asp130 135 140Asp Leu Val Ala Tyr Cys Arg Trp Trp Lys Pro Asp Leu Val Val Trp145 150 155 160Glu Trp Leu Ser His Ala Gly Ala Val Ala Ala Ala Ala Val Gly Ala165 170 175Ala His Ala Arg Met Pro Ile Gly Ile Glu Val Glu Ala Arg Met Arg180 185 190Arg His Phe Leu Lys Leu Leu Ala Gln Gln Glu Pro Ala Asp Arg Glu195 200 205Asp Pro Met Ala Glu Trp Leu Gly Ala Trp Gly Glu Lys His Gly Phe210 215 220Glu Phe Arg Glu Glu Leu Val Thr Gly Gln Phe Thr Ile Asp Gln Val225 230 235 240Pro Asp Ser Met Arg Leu Lys Ala Asn Val Pro Gln Val Ser Val Arg
245 250 255His Val Pro Tyr Asn Gly Arg Ala Val Ala Pro Asp Trp Ile Arg Pro260 265 270Glu Pro Pro Ala Pro Arg Val Leu Ala Thr Phe Gly Met Ser Met Trp275 280 285Asp Met Ser Ser Ser Gln Pro Val Ser Ser Tyr Gln Met Val Ser Ile290 295 300Glu Gln Leu Gln Asp Trp Leu Asp Ser Met Ala Asp Leu Glu Met Glu305 310 315 320Leu Val Met Thr Leu Pro Gly Arg Ile Gln Glu Lys Leu Lys His Val325 330 335Pro Gln Asn Thr Arg Leu Val Asp Phe Val Pro Leu His Leu Val Ile340 345 350Pro Ser Cys Ala Ala Val Ile His His Gly Gly Leu Pro Ala Phe Cys355 360 365Ser Ser Leu Ala His Gly Val Pro Gln Leu Met Val Ser Arg Met Ala370 375 380Pro Asp Ala Ser Val Arg Gly Ala Arg Leu Glu Glu Ala Gln Ala Gly385 390 395 400Gly Trp Ile Pro Pro Glu Arg Met Thr Gly Lys Arg Ile Arg Asn His405 410 415Leu Ala Lys Leu Val Gln Asp Pro Ser Tyr Arg Ala Gly Ala Glu Arg420 425 430Leu Arg Gln Glu Val Leu Ala Gln Pro Ser Pro Asn Glu Val Val Pro435 440 445Glu Leu Glu Arg Leu Thr Glu Glu Leu Arg Ser Arg450 455 460<210> 16<211> 305<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Ala Gln Gly Phe Gln Ala Ser Leu Gln Trp Glu Arg Ile Asn Glu1 5 10 15Leu Trp Val Thr Glu Glu Ala Ser Ala Asp Leu Thr Gly Phe Lys Ser20 25 30Asp Arg Arg Asn Phe Asn Ile Ala Leu Trp Asp Pro Thr Thr Asn Gly35 40 45Ile Arg Tyr Leu Arg Ala Leu Val Tyr Glu Leu Ala Thr Arg Leu Ser50 55 60Asp Asp Asp Trp Ser Lys Ile Glu Lys Val Arg Asn Arg Asp Val Gly65 70 75 80Asp Pro Val Thr Val Arg Tyr Glu Gly Arg Thr Val Cys Leu Asp Tyr85 90 95Leu Gln Ala Ala Leu Glu Leu Gly Phe Ile Glu Lys Glu Leu Asp Leu100 105 110Gly Gly Ala Arg Val Leu Glu Ile Gly Ala Gly Tyr Gly Arg Thr Cys115 120 125His Ala Met Leu Ser Asn Tyr Asp Leu Ala Ser Tyr Thr Ile Val Asp130 135 140Leu Lys Asn Thr Leu Gly Leu Ser Arg Ala Tyr Leu Arg Glu Val Leu145 150 155 160Asp Glu Lys Gln Phe Ser Lys Met Arg Phe Val Gln Val Glu Asp Ile165 170 175Asp Thr Gly Leu Gly Pro Asp Gly Phe Asp Leu Cys Val Asn Val His180 185 190Ser Phe Thr Glu Met Thr Pro Asp Thr Val Lys Ala Tyr Leu Arg Leu195 200 205Ile Asp Glu Arg Cys Gly Ala Phe Phe Val Lys Asn Pro Val Gly Lys210 215 220Phe Arg Asp Lys Ser Met Asp Gly His Gln Lys Gly Glu Glu Ala Val225 230 235 240Arg Leu Ala Met Gln Thr Gly Pro Leu Arg Gln Val Leu Asp Ile His245 250 255Asp Ser Gln Ala Val Ala Ala Ala Val Pro Ala Phe Ile Glu Ala Tyr260 265 270Gln Pro Gly Glu Gly Trp Thr Cys Ala Ala Asn Thr Arg Gly Met Pro275 280 285Trp Ser Tyr Phe Trp Gln Ala Leu Tyr Thr Lys Thr Gly Asp Asp Leu290 295 300Arg305<210> 17<211> 346<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Ala Ala Ser Pro Val Pro Pro Pro Arg Gly Asp Glu Ala Leu Ala1 5 10 15Gly Thr Pro Val Leu Val Leu Gly Gly Ser Gly Tyr Leu Gly Arg His20 25 30Ile Cys Ser Ala Phe Gly Ala Ala Gly Ala Gln Val Val Pro Val Ser35 40 45Arg Gly Ala Arg Gly Gly Val Asp Gly Asp Gly Cys Arg Ser Val Arg50 55 60Leu Asp Leu Thr Ala Ala Gly Pro Asp Glu Leu Ala Arg Leu Cys Ala65 70 75 80Gly Thr Gly Ala Arg Val Leu Val Asn Ala Ser Gly Ala Val Trp Gly85 90 95Gly Gly Glu Arg Gln Met Ala Glu Ala Asn Thr Glu Leu Val Gly Arg100 105 110Leu Ala Gly Ala Val Ala Arg Leu Pro Gly Arg Pro Arg Leu Ile His115 120 125Leu Gly Ser Ala Tyr Glu Tyr Gly Pro Ala Arg Pro Gly Thr Ala Ile130 135 140Ala Glu Asp Trp Pro Pro Ala Pro Thr Thr Val Tyr Gly Arg Thr Lys145 150 155 160Leu Ser Gly Ser Gln Ala Val Leu Arg Ala Ala Ala Glu Leu Gly Val165 170 175Ala Gly Thr Val Leu Arg Val Ser Val Ala Cys Gly Pro Gly Ala Pro180 185 190Val Ser Ser Leu Ala Gly Ala Val Ala Ala His Leu Ala Ala Gly Arg195 200 205Asp Glu Leu Arg Leu Ala Pro Leu Arg Asp His Arg Asp Leu Val Asp210 215 220Val Arg Asp Val Ala Asp Ala Val Val Ala Ala Ala Val Ala Pro Val225 230 235 240Ala Ala Val Thr Gly Thr Val Val Asn Ile Gly Ser Gly Gln Ala Val245 250 255Pro Val Arg Arg Leu Val Asp Leu Met Ile Ala Leu Ser Gly Arg Pro260 265 270Val Arg Val Ile Glu Asp Pro Ala Leu Arg Arg Thr Arg Ser Asp Ala275 280 285Ala Trp Gln Arg Leu Asp Ile Gly Arg Ala Arg Arg Leu Leu Gly Trp290 295 300Ala Pro Arg Arg Thr Leu Arg Glu Ser Leu Arg Asp Leu Leu Ala Ala305 310 315 320Val Gly Ala Pro Gln Pro Ala Ala Val Arg Ala Ala Thr Ala Ile Gly325 330 335Pro Arg Asn Ser His Gly Lys Asp Ser Arg340 345<210> 18<211> 434<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Ser Glu Arg Val Ala Arg Ile Leu Asp Glu Val Arg Lys Tyr His1 5 10 15Gln Asp Ser Gln Glu Gly Arg Gly Phe Ile Pro Gly Val Thr Glu Ile20 25 30Trp Pro Ser Gly Ala Val Leu Asp Glu Asp Asp Arg Val Ala Leu Val35 40 45Gln Ala Ala Leu Glu Met Arg Ile Ala Ala Gly Lys Leu Ser Arg Lys50 55 60Phe Glu Ser Ala Phe Ala Arg Arg Met Lys Arg Arg Lys Ala His Leu65 70 75 80Thr Asn Ser Gly Ser Ser Ala Asn Leu Leu Ala Ile Ser Ala Leu Thr85 90 95Ser His Leu Leu Gly Glu Arg Arg Leu Arg Pro Gly Asp Glu Val Ile100 105 110Thr Val Ala Ala Ser Phe Pro Thr Thr Val Asn Pro Ile Leu Gln Asn115 120 125Gly Leu Val Pro Val Tyr Val Asp Val Glu Leu Gly Thr Tyr Asn Ala130 135 140Thr Ala Glu Arg Val Ala Glu Ala Ile Gly Pro Arg Thr Arg Ala Ile145 150 155 160Met Met Ala His Thr Leu Gly Asn Pro Phe Gln Ala Thr Glu Met Ala165 170 175Arg Leu Ala Gln Asp His Asp Leu Ile Leu Ile Glu Asp Ser Cys Asp180 185 190Ala Val Gly Ser Thr Tyr Asp Gly Arg Pro Ala Gly Thr Phe Gly Asp195 200 205Leu Thr Thr Val Ser Phe Tyr Pro Ala His His Leu Thr Met Gly Glu210 215 220Gly Gly Cys Val Leu Thr Ser Asn Leu Val Leu Ala Arg Ile Val Glu225 230 235 240Ser Leu Arg Asp Trp Gly Arg Asp Cys Trp Cys Glu Pro Gly Glu Ser
245 250 255Asp Thr Cys Arg Lys Arg Phe Gly Tyr Gln Met Gly Thr Leu Pro Ala260 265 270Gly Tyr Asp His Lys Tyr Ile Phe Ser His Ile Gly Tyr Asn Leu Lys275 280 285Ser Thr Asp Leu Gln Ala Ala Leu Gly Leu Thr Gln Leu Asp Lys Leu290 295 300Asp Ala Phe Cys Ser Ala Arg Arg Ser Asn Trp Arg Arg Leu Arg Glu305 310 315 320Gly Leu Asp Gly Leu Pro Trp Leu Ile Leu Pro Glu Ala Thr Pro Arg325 330 335Ser Asp Pro Ser Trp Phe Gly Phe Val Leu Thr Val Asp Pro Arg Ala340 345 350Pro Phe Ser Arg Ala Glu Leu Val Asp Phe Leu Glu Ser Arg Lys Ile355 360 365Gly Thr Arg Arg Leu Phe Ala Gly Asn Leu Thr Arg His Pro Ala His370 375 380Ala Glu Ala Pro His Arg Val Cys Gly Asp Leu Ala Asn Ser Asp Thr385 390 395 400Val Thr Glu His Thr Phe Trp Val Gly Val Tyr Pro Gly Leu Thr Glu405 410 415Glu Met Ile Asp Phe Met Val Ser Ser Ile Thr Glu Phe Ile Gly Ser420 425 430His Arg<210> 19<211> 331<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Lys Leu Leu Val Thr Gly Ala Ala Gly Phe Ile Gly Ser Thr Tyr1 5 10 15Ala Arg Arg Leu Leu Ala Arg Gly Gly Ala Glu Trp Gly Pro Asp Val20 25 30Ser His Val Thr Val Leu Asp Lys Leu Thr Tyr Ala Gly Thr Leu Ser35 40 45Asn Leu Asp Thr Ala Asp Pro Arg Leu Thr Phe Val His Gly Asp Ile50 55 60Cys Asp Ala Asp Leu Val Asp Thr Leu Met Ala Arg Ala Asn Gln Val65 70 75 80Val His Phe Ala Ala Glu Ser His Val Asp Arg Ser Ile Thr Gly Ala85 90 95Asp Pro Phe Val Arg Thr Asn Val Glu Gly Thr His Thr Leu Leu Gln
100 105 110Ala Ala Leu Arg His Gly Val Glu Arg Phe Val His Val Ser Thr Asp115 120 125Glu Val Tyr Gly Ser Val Glu Thr Gly Phe Ser Pro Glu Thr Ala Val130 135 140Leu Asp Pro Asn Ser Pro Tyr Ala Ala Ser Lys Ala Ala Ser Asp Leu145 150 155 160Ile Ala Leu Ala Tyr His Arg Thr His Gly Leu Asp Val Arg Val Thr165 170 175Arg Cys Ser Asn Asn Tyr Gly Pro His Gln Phe Pro Glu Lys Ile Ile180 185 190Pro Leu Phe Ile Thr Asn Leu Leu Asp Gly Glu Asp Val Pro Leu Tyr195 200 205Gly Asp Gly Leu Asn Val Arg Asp Trp Leu His Val Glu Asp His Cys210 215 220Arg Gly Val Glu Leu Val Arg Thr Lys Gly Ser Pro Gly Glu Ile Tyr225 230 235 240Asn Ile Gly Gly Gly Thr Ala Leu Thr Asn Arg Glu Leu Thr Gly Arg245 250 255Leu Leu Asp Ala Cys Gly Ala Gly Trp Ser Arg Val Arg Tyr Val Glu260 265 270Asp Arg Lys Gly His Asp Arg Arg Tyr Ala Val Gln Asp Asp Lys Ala275 280 285Arg Asp Glu Leu Gly Tyr Arg Pro Arg His Asp Phe Ala Ala Gly Leu290 295 300Ala Glu Thr Val Ala Trp Tyr Arg Asp Asn Arg Pro Trp Trp Glu Pro305 310 315 320Leu Lys Arg Ser Ala Leu Thr Gly Gly Ser Arg325 330<210> 20<211> 302<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Lys Gly Ile Ile Leu Ala Gly Gly Asn Gly Thr Arg Leu Gln Pro1 5 10 15Leu Thr Leu Ala Gly Ser Lys Gln Leu Val Pro Val Tyr Asp Lys Pro20 25 30Met Ile Tyr Tyr Pro Leu Ser Val Leu Met Phe Ala Gly Ile Arg Asp35 40 45Ile Leu Ile Ile Ser Arg Pro Thr Glu Leu Pro Gln Phe Arg Gln Leu50 55 60Phe Gly Asp Gly Arg Arg Leu Gly Leu Asn Leu Ser Tyr Ala Ser Gln65 70 75 80Glu Lys Pro Arg Gly Ile Ala Asp Ala Phe Arg Ile Gly Ala Asp His85 90 95Ile Arg Gly Glu Glu Cys Ala Leu Ile Leu Gly Asp Asn Leu Phe His100 105 110Gly Ala Asn Leu Pro Ala Leu Leu Arg Arg Ser Val Gln Arg Leu Arg115 120 125Gly Cys Val Leu Phe Gly His Glu Val Ala Asp Pro Arg His Phe Gly130 135 140Val Ala Glu Ile Asp Glu Arg Gly Arg Leu Leu Ser Ile Glu Glu Lys145 150 155 160Pro Glu Arg Pro Arg Ser Asn Leu Ala Ile Pro Gly Leu Tyr Leu Phe165 170 175Asp Gly Gly Val Val Asp Val Ala Lys Arg Leu Val Pro Ser Ala Arg180 185 190Gly Glu Leu Glu Ile Thr Asp Val Leu Arg Ala Tyr Leu Glu Glu Gly195 200 205Thr Ala Asp Leu Val Trp Leu Gly Arg Gly Val Thr Trp Leu Asp Thr210 215 220Gly Thr His Glu Thr Leu Leu Asp Ala Gly Arg Met Val Arg Asp Val225 230 235 240Gln His Tyr Gln Gly Thr Arg Leu Gly Cys Val Glu Glu Ile Ala Met245 250 255Tyr Met Gly Phe Ile Gly Ala Asp Glu Cys Tyr Thr Leu Gly Thr Glu260 265 270Met Ser Asn Ser Pro Tyr Gly Gln Tyr Val Met Asp Arg Ala Arg Ser275 280 285Tyr Arg Arg Gly Leu Pro Pro Ala Arg Phe Leu Glu Glu Ile290 295 300<210> 21<211> 1646<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Ala Asn Asp Asp Lys Lys Leu Leu Asp Tyr Leu Lys Arg Val Thr1 5 10 15Ala Asp Leu Arg Glu Ala Gln Arg Arg Leu Lys Asp Val Glu Tyr Ala20 25 30Arg His Glu Pro Val Ala Ile Ile Gly Ile Gly Cys Arg Phe Pro Gly35 40 45Asp Ala Gly Ser Pro Glu Asp Leu Trp Asp Leu Val Ala Ala Gly Arg50 55 60Glu Gly Thr Gly Gly Leu Pro Ala Asp Arg Gly Trp Asp Leu Glu Ala65 70 75 80Leu Tyr Asp Pro Glu Pro Gly His Pro Gly Thr Ser Tyr Val Arg Glu85 90 95Gly Gly Phe Leu Arg Asp Ala Ala Arg Phe Asp Ala Ala Phe Phe Gly100 105 110Ile Ser Pro Asn Glu Ala Val Thr Met Ala Pro Gln Gln Arg Leu Ala115 120 125Leu Glu Leu Ala Trp Glu Ala Val Glu His Ala Arg Ile Asp Pro His130 135 140Thr Leu Arg Ser Ser Ala Thr Gly Thr Tyr Leu Gly Cys Asp Gly Leu145 150 155 160Asp Tyr Phe Leu Asn Ser Phe Gln Val Pro Glu Gly Ala Ala Gly Gln165 170 175Leu Thr Thr Gly Asn Ser Pro Ser Val Val Ala Gly Arg Val Ser Tyr180 185 190Thr Leu Gly Leu Glu Gly Ala Ala Val Thr Leu Asp Thr Ala Cys Ser195 200 205Ser Ala Leu Val Ala Ile His Leu Ala Cys Gln Ala Leu Arg Glu Glu210 215 220Glu Val Thr Leu Ala Leu Ala Gly Gly Val Tyr Val Met Ser Ser Pro225 230 235 240Ala Pro Leu Val Gly Phe Ser Glu Leu Arg Ala Leu Ala Pro Asp Gly245 250 255Arg Ala Lys Pro Phe Ser Ala Asp Ala Asp Gly Met Asn Leu Ala Glu260 265 270Gly Ala Gly Val Val Leu Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn275 280 285Gly His Glu Val Leu Ala Val Ile Arg Gly Ser Ala Val Asn Glu Asp290 295 300Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg305 310 315 320Val Ile Gln Gln Ala Leu Ala Asn Ala Arg Leu Ala Pro Ala Asp Val325 330 335Asp Ala Val Glu Ala His Gly Thr Gly Thr Ser Leu Gly Asp Pro Ile340 345 350Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg Pro Glu Gly355 360 365Arg Pro Leu Trp Leu Gly Ser Ile Lys Ser Asn Ile Gly His Thr Gln370 375 380Ile Ala Ala Gly Ala Ala Gly Val Ile Lys Met Val Met Ala Leu Arg385 390 395 400His Gly Met Leu Pro Arg Ser Leu His Ile Asp Glu Pro Thr Pro His405 410 415Val Ala Trp Asp Thr Gly Gly Val Arg Leu Leu Asp Gln Ala Val Glu420 425 430Trp Pro Arg Ala Glu Arg Pro Arg Arg Ala Gly Val Ser Ala Phe Gly
435 440 445Phe Ser Gly Thr Asn Ala His Leu Ile Leu Glu Glu Ala Pro Glu Pro450 455 460Ala Pro Pro Ala Asn Glu Ser Ala Glu Pro Ala Glu Pro Ala Ala Leu465 470 475 480Val Thr Pro Trp Val Leu Ser Ala Arg Ser Ala Ala Ala Leu Arg Arg485 490 495Gln Ala Arg Arg Leu Leu Asp Ala Ala Thr Asp Gly Asp Pro Arg Ala500 505 510Val Gly Trp Ser Leu Val Thr Thr Arg Ser Val Phe Glu His Arg Ala515 520 525Val Val Thr Gly Pro Asp Gly Ala Thr Leu Arg Glu Arg Leu Ala Ala530 535 540Leu Ala Ala Gly Glu Pro Ala Pro His Val Val Thr Gly Ala Pro Gly545 550 555 560Ala Pro Gly Ala Gly Val Val Leu Val Phe Pro Gly Gln Gly Ser Gln565 570 575Trp Pro Gly Met Gly Ala Glu Leu Leu Asp Ala Ser Pro Val Phe Ala580 585 590Ala Arg Ile Ala Glu Cys Glu Ala Ala Leu Ala Pro Tyr Val Asp Trp595 600 605Ser Leu Thr Glu Val Leu Arg Gly Ala Asp Gly Ala Ala Asp Leu Gly610 615 620Arg Val Asp Val Val Gln Pro Val Leu Trp Ala Leu Met Val Ser Leu625 630 635 640Ala Ala Leu Trp Ala His His Gly Val Arg Pro Ala Ala Val Val Gly645 650 655His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Thr660 665 670Leu Glu Asp Gly Ala Arg Val Val Ala Leu Arg Ser Gln Ala Leu Arg675 680 685Ala Leu Ala Gly Arg Gly Ala Met Ala Ser Leu Gly Val Asp Pro Asp690 695 700Thr Ala Glu Arg Leu Val Ala Glu Val Gly Glu Lys Ala Ala Gly Val705 710 715 720Gly Val Ala Ala Leu Asn Ser Pro Ser Ser Thr Val Val Ser Gly Pro725 730 735Pro Asp Ala Val Ala Ala Val Val Ala Ala Cys Glu Ala Thr Gly Ala740 745 750Arg Ala Arg Thr Ile Asp Val Asp Tyr Ala Ser His Gly Pro Gln Val755 760 765Asp Glu Ile Ala Asp Glu Val Thr Ala Arg Leu Ala Gly Val Gly Gly770 775 780Gln Ala Thr Asp Val Ala Phe Tyr Ser Thr Val Thr Gly Gly Pro Met785 790 795 800Asp Thr Ala Thr Ala Leu Asp Ala Gly Tyr Trp Leu Thr Asn Leu Arg805 810 815Arg Pro Val Arg Leu Thr Glu Ala Val Asp Ala Leu Leu Thr Ala Gly820 825 830His Arg Val Phe Ile Glu Val Ser Thr His Pro Val Val Val Pro Ala835 840 845Leu Gln Gln Cys Phe Glu Ser Ala Gln Val Ala Ala Val Ala Met Gly850 855 860Thr Leu Arg Arg Asp Glu Gly Gly Pro Ala Gln Leu Ala Thr Ala Leu865 870 875 880Ala Gln Ala Phe Thr Ser Gly Val Pro Val Asp Trp Arg Pro Trp Phe885 890 895Asp Gly Pro Pro Ala Pro Arg Thr Thr Ala Leu Pro Thr Tyr Ala Phe900 905 910Asp Arg Glu Arg Tyr Trp Leu Pro Pro Ala Arg Ala Val Arg Gly Asp915 920 925Gly Ala Gln Asp Pro Ala Glu Ala Glu Leu Trp Gly Ala Ile Glu Asp930 935 940Leu Asp Thr Glu Ala Leu Ala Arg Val Leu Glu Pro Asp Gly Thr Ala945 950 955 960Glu Asp Ile Glu Ala Leu Arg Pro Ala Leu Pro Val Leu Ser Thr Trp965 970 975Arg Arg Arg His Arg Glu Arg Thr Thr Leu Glu Ser Trp Arg His Gln980 985 990Ile Arg Trp Thr Pro Leu Pro Asp Pro Ala Pro Pro Ala Val Ser Ser995 1000 1005Thr Trp Leu Leu Leu Val Pro Thr Gly Tyr Glu Asp His Pro Ala1010 1015 1020Val Arg Thr Val Thr Gly Ala Leu Thr Gly His Gly Ala Glu Val1025 1030 1035Arg Pro Cys Pro Ala Asp Pro Ser Val Thr Ser Arg Ala Glu Leu1040 1045 1050Ala Glu Arg Leu Thr Gly Leu Arg Gly Asp Asp Ala Pro Ala Gly1055 1060 1065Val Val Ser Leu Leu Ala Leu Asp Glu Arg Pro Arg Ala Asp His1070 1075 1080Pro Ala Val Pro Ala Gly Leu Ala Ala Thr Val Ala Ala Leu Gln1085 1090 1095Ala Leu Gly Asp Ala Gly Ile Thr Ala Pro Leu Trp Cys Val Thr1100 1105 1110Gln Gly Ala Val Ser Thr Gly His Asp Asp Pro Leu Ser His Pro
1115 1120 1125Leu Gln Ala Gln Thr Trp Gly Leu Gly Arg Val Ala Ala Leu Glu1130 1135 1140His Pro Asp Arg Trp Gly Gly Leu Val Asp Leu Pro Ala Glu Pro1145 1150 1155Asp Glu Arg Thr Glu Ala Gln Leu Ala Ala Leu Leu Ala Gly Ala1160 1165 1170Pro Thr Gly Gly Gln Ala Glu Asp Gln Val Ala Ile Arg Ala Gly1175 1180 1185Gly Gly Ala Leu Ala Arg Arg Leu Val His Thr Thr Ser Ala Arg1190 1195 1200Thr Ala Asp Gln Ala Trp Gln Pro Arg Gly Ser Val Leu Ile Thr1205 1210 1215Gly Gly Thr Gly Gly Val Gly Ala Leu Leu Ala Arg Trp Ala Ala1220 1225 1230Glu Arg Gly Ala Ala Gln Leu Val Leu Thr Ser Arg Arg Gly Pro1235 1240 1245Asn Ala Pro Gly Ala Ala Glu Leu Ala Ala Glu Leu Arg Gly Leu1250 1255 1260Gly Val Ala Val Thr Ile Ala Ala Cys Asp Ala Ala Asp Pro Glu1265 1270 1275Ala Met Arg Gly Val Leu Asp Ala Ile Pro Ala Glu His Pro Leu1280 1285 1290Ser Ala Val Ile His Ala Ala Gly Val Ser Glu Gln Asp Leu Ile1295 1300 1305Ala Asp Val Asp Asp Glu His Leu Asn Arg Met Leu Ala Pro Lys1310 1315 1320Ala Leu Ala Ala Trp His Leu His Glu Leu Thr Arg His Leu Asp1325 1330 1335Leu Ser Ala Phe Ile Leu Phe Ser Ser Val Ser Ala Ser Trp Gly1340 1345 1350Ser Gly Gln Gln Ala Gly Tyr Ala Ala Ala Asn Ala Tyr Leu Asp1355 1360 1365Ala Leu Ala Glu His Arg Arg Gly Leu Gly Leu Pro Thr Thr Ser1370 1375 1380Val Ala Trp Gly Leu Trp Gly Glu Val Gly Met Ala Thr Thr Ala1385 1390 1395Asp Glu Val Asp Ala Phe Arg Arg Arg Gly Ile His Pro Leu Asp1400 1405 1410Pro Gly Leu Ala Ile Ala Ser Leu Gln Gln Ala Val Glu Arg Arg1415 1420 1425Glu Thr Asn Ala Val Val Ala Ala Ile Asp Trp Arg Arg Phe Leu1430 1435 1440Thr Gly Phe Thr Ala Leu Arg Pro Ser Pro Leu Leu Ser Asp Leu1445 1450 1455Pro Glu Ala Ala Glu Ala Arg Ala Glu Glu Thr Arg Pro Arg Glu1460 1465 1470Asp Glu Ala Asp Pro Leu Arg Arg Lys Leu Ala Gly Cys Pro Pro1475 1480 1485Ala Glu Gln His His Ile Leu Val Arg His Val Gln Ala His Ala1490 1495 1500Ala Thr Thr Leu Gly His Ala Asp Ala Asp Ala Val Pro Pro Thr1505 1510 1515Lys Pro Phe Gln Glu Leu Gly Phe Asp Ser Leu Thr Ala Val Gln1520 1525 1530Leu Arg Asp Arg Leu Asn Ala Gly Thr Gly Leu Arg Leu Pro Thr1535 1540 1545Thr Val Leu Phe Asp Tyr Pro Ser Ala Glu Glu Leu Ala Arg His1550 1555 1560Leu His Gly Leu Leu Val Ala Asp Ala Ala Ser Gly Glu Gln Leu1565 1570 1575Ile Met Ser Glu Leu Asp Gly Trp Asp Ala Ala His Ala Pro Asp1580 1585 1590Thr Val Asp Glu Ala Ala Cys Ser Arg Ile Ala Ala Arg Leu Arg1595 1600 1605Leu Leu Ala Asp Lys Trp Ser Asp Thr Ala Arg Ala Thr Gly Ser1610 1615 1620His Ser Asp Leu Glu Thr Ala Thr Ala Glu Asp Ile Phe Asp Leu1625 1630 1635Ile Ala Thr Glu Phe Gly Lys Ser1640 1645<210> 22<211> 290<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Leu Ser Val Pro Val Asp His Ala Val His Leu Asn Val Arg His Arg1 5 10 15Pro Gly Arg Asp Gly Arg Pro Phe Leu Leu Leu His Gly Leu Gly Ser20 25 30Asn Ala Arg Leu Trp Asp Glu Val Ala Asp Leu Leu Ala Ala Ala Gly35 40 45His Pro Val Tyr Ala Leu Asp Met Arg Gly His Gly Asp Ser Asp Leu50 55 60Pro Glu His Gly Tyr Asp Asn Ala Thr Ala Val Ala Asp Leu Val Ala65 70 75 80Val Cys Arg Glu Leu Arg Leu Thr Gly Ala Leu Phe Ala Gly His Ser85 90 95Trp Gly Gly Asn Leu Ala Val Arg Leu Ala Ala Gln His Pro Glu Leu100 105 110Ala Ala Gly Leu Ala Leu Val Asp Gly Gly Trp Ile Gly Phe Val Asp115 120 125Thr Ser Arg Tyr Ala Pro Thr Arg Glu Lys Ser Val Glu Leu Ala Gly130 135 140Trp Trp Arg Asp Val Thr Gly Ile Lys Glu Glu Thr Met Arg Glu Leu145 150 155 160Leu Arg Gly Leu His Pro Thr Trp Ser Gln Thr Ala Val Glu Ala Ser165 170 175Leu Ala Asp Met Val Glu Gly Pro Asp Gly Leu Leu Val Gln Arg Leu180 185 190Pro Leu Glu His Tyr Met Ser Leu Ala Asp Ser Met Trp Gln Asp Pro195 200 205Pro Ala Arg Trp Tyr Pro Gly Ile Thr Ala Pro Val Leu Leu Leu Val210 215 220Ala Leu Pro Ala His Ala Gln Ser Trp Gly Thr Tyr Ala Arg Lys Trp225 230 235 240Val Ala Glu Ala Glu Ala Ala Ile Pro Gln Ala Glu Ser Arg Trp Tyr245 250 255Val Asp Thr Asp His Asn Leu His Val Glu Glu Pro Glu Arg Val Ala
260 265 270Ser Asp Leu Leu Asp Leu Ala Arg Leu Val Asp Lys Pro Ala Pro Glu275 280 285Arg Ser290<210> 23<211> 104<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Glu Pro Asn Glu Glu Ala Thr His Asp Glu Gln Leu Val Ala Arg1 5 10 15Leu Ala Ser Ala Thr Pro Glu Glu Arg Gln Arg Leu Leu Ala Ala Gln20 25 30Val Leu Arg Arg Ala Ser Glu Val Leu Asp Val Pro Ala Leu Asp Glu35 40 45Glu Ser Asn Phe Leu Glu Asn Gly Leu Ser Ser Leu Ser Ala Val Gln50 55 60Leu Ala Lys Ser Leu Met Ser Asp Thr Gly Leu Glu Val Pro Leu Val65 70 75 80Ala Ile Val Glu His Pro Thr Ser Thr Leu Leu Gly Lys Tyr Leu Ala85 90 95Glu Thr Tyr Glu Ala Asp Ala Ala
100<210> 24<211> 478<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Thr Thr Pro Thr Arg Ala Val Val Leu Gly Gly Gly Trp Ala Gly1 5 10 15Met Leu Thr Ala His Val Leu Ala Arg His Leu Glu Ser Val Thr Val20 25 30Val Glu Arg Asp Ile Leu Pro Asp Gly Pro His His Arg Lys Gly Gln35 40 45Pro Gln Ala Arg His Val His Val Leu Trp Ser Ser Gly Ala Gly Ile50 55 60Val Glu Asn Leu Leu Pro Gly Thr Ala Glu Arg Leu Leu Ala Ala Gly65 70 75 80Ala Arg Arg Ile Gly Phe Gln Ser Asp Leu Val Thr Leu Thr Ala Trp85 90 95Gly Trp Gln Tyr Arg Phe Pro Ala Thr Ala Tyr Ala Met Met Cys Thr100 105 110Arg Pro Leu Leu Asp Trp Val Val Arg Asp Ala Ile Leu Ala Gly Gly115 120 125Arg Ile Glu Val Glu His Gly Thr Glu Ala Val Glu Leu Ala Gly Asp
130 135 140Arg Ser Arg Val Thr Gly Val Arg Val Arg Asp Ala Gly Gly Gly Glu145 150 155 160Pro Arg Leu Leu Glu Ala Asp Leu Val Val Asp Ala Thr Gly Arg Ala165 170 175Ser Arg Leu Gly His Trp Leu Ala Ala Leu Gly Leu Pro Ala Val Glu180 185 190Gln Asp Val Val Asp Ala Gly Ile Gly Tyr Ala Thr Arg Met Phe Lys195 200 205Ala Pro Glu Gly Ala Asp Gly Asn Phe Pro Ala Val Gln Val Ala Ala210 215 220Asp Pro Leu Thr Arg Gln Pro Gly Arg Phe Gly Val Val Tyr Pro Gln225 230 235 240Glu Gly Gly Arg Trp Leu Val Thr Leu Thr Ser Thr Arg Gly Ala Pro245 250 255Leu Pro Thr Asp Glu Asp Glu Phe Thr Gly Tyr Ala Lys Val Leu Arg260 265 270His Ser Ile Val Ser Glu Leu Met Ser Val Ala Glu Pro Ile Ser Pro275 280 285Ile Phe Gln Ser His Ser Gly Ala Asn Arg Arg Met Tyr Pro Glu Arg290 295 300Met Pro Gln Trp Pro Glu Gly Leu Leu Ile Leu Gly Asp Ser Leu Ala305 310 315 320Ala Phe Asn Pro Val Tyr Gly His Gly Met Ser Ser Ala Ala Arg Ala325 330 335Ala Glu Ala Leu Asp Lys Glu Leu Ala Arg Asp Gly Phe Gly Glu Gly340 345 350Gly Thr Arg Gln Val Gln Arg Ala Leu Ser Glu Val Val Asp Asp Pro355 360 365Trp Ile Met Ala Gly Leu Asn Asp Ile Gln Tyr Val Asn Cys Arg Asn370 375 380Leu Ser Ser Asp Pro Arg Leu Thr Gly Pro Asp Val Ala Glu Arg Leu385 390 395 400Lys Phe Ser Asp Phe Leu Ser Gly Lys Ser Ile Arg Ser Pro Lys Val405 410 415Cys Glu Val Thr Thr Ser Val Leu Ser Leu Asn Ala Pro Gln Lys Ala420 425 430Leu Gly Asp Ser Arg Phe Leu Ser Leu Leu Arg Thr Asp Thr Ser His435 440 445Pro Lys Leu Val Glu Pro Pro Phe His Pro Glu Glu Leu Glu Met Val450 455 460Gly Leu Lys Pro Ser Gly Ile Ala Ala Lys Gly Ala Leu Gly465 470 475<210> 25<211> 313<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Val Leu Asn Glu Arg Thr Arg Lys Lys Ile Ala Arg Glu His Ser Arg1 5 10 15Arg Leu Ser Glu Gly Asp Ile Asp Gly Leu Leu Asp Leu Tyr Ala Lys20 25 30Asp Val Thr Phe Glu Ala Pro Val Gly Ala Gly Gly Gln Ala Gly His35 40 45Glu Ala Leu Arg Ala His Phe Glu Ser Ala Val Ala Gly Asn Val Asp50 55 60Glu Thr Ile Val Glu Ser Val Val Gly Gln Asp Gly Glu His Val Leu65 70 75 80Ser Arg Ile Thr Ala Val Met Asp Tyr Arg Pro Arg Gly Pro Leu Tyr85 90 95Ala Asp Arg Gly Trp Leu Pro Ala Pro Glu Gly Ala Glu Pro Thr Ala100 105 110Leu Arg Cys His Tyr Ala Leu Leu Leu Arg Val Gly Glu Ser Gly Leu115 120 125Ile Glu Asp Met Arg Ala Tyr Trp Gly Lys Pro Asp Leu Glu Thr Ser130 135 140Val Asp Gly Arg Ser Gly Pro Phe Val Gly Pro Pro Ala Ile Asp Pro145 150 155 160Asp Glu Ala Ala Leu Arg Glu Leu Pro His Asn Tyr Leu Arg Leu Leu165 170 175Gln Lys Gly Asp Val Glu Gly Thr Val Ala Leu Phe Thr Asp Asp Ile180 185 190Val Phe Glu Asp Pro Val Gly Gly Leu Leu Leu Arg Gly Lys Asp Ala195 200 205Leu Arg Glu His Ala Phe Arg Gly Ser Glu Gly Lys Val His Glu Met210 215 220Leu Gly Arg Leu Val Thr Ser Met Asp Gly Arg Phe Val Val Val Leu225 230 235 240Gly Asp Ala Arg Val Tyr Val Pro Ala Arg Met Arg Met Arg Met Ile245 250 255Thr Ile Cys Glu Val Asn Glu Asp Arg Leu Gly Ala His Ile Gln Gly260 265 270Phe Trp Gly Leu Thr Asp Met Thr Ile Gly Phe Pro Asp Asp Asp Thr275 280 285Ala Pro Glu Pro Ala Leu Asp Thr Ala Arg Arg Pro Glu Arg Lys Asp290 295 300Ala Leu Lys Pro Phe Gly Thr Pro Ser305 310<210> 26<211> 802<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Val Pro Ser Leu Arg Arg Asn Val Met Thr Val Ala Asp Ala Lys Val1 5 10 15Val Glu Ala Leu Arg Thr Ser Leu Leu Glu Thr Glu Arg Leu Arg Lys20 25 30Glu Asn Asp Arg Leu Arg Ala Ala Pro Arg Glu Pro Val Ala Ile Thr35 40 45Gly Met Ala Cys Arg Tyr Pro Gly Gly Val Glu Ser Pro Asp Asp Leu50 55 60Trp Thr Leu Leu Thr Glu Glu Arg Asp Ala Ile Gly Ala Phe Pro Ala65 70 75 80Asp Arg Gly Trp Asp Leu Asp Gly Leu Tyr Gly Pro Asp Ala His Pro85 90 95Asp Val Arg Ser Ala Val Glu Glu Gly Gly Phe Leu Ser Asp Ala Gly100 105 110Ala Phe Asp Pro Ala Pro Phe Gly Ile Ser Pro Gly Glu Ala Ser Val115 120 125Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Thr Trp Glu Ala Phe130 135 140Glu Arg Ala Gly Ile Asp Pro Arg Ser Val Arg Gly Arg Arg Cys Gly145 150 155 160Val Phe Met Gly Thr Thr Gly Gln Asp Tyr Thr Pro His Leu Lys Asp165 170 175Val Pro Asp Glu Leu Leu Gly His Ile Ala Ser Gly Gly Ser Ser Ala180 185 190Val Leu Ser Gly Arg Leu Ala Ser Val Phe Gly Leu Glu Gly Pro Thr195 200 205Ala Thr Leu Asp Thr Ala Cys Ser Gly Ser Leu Val Ala Leu His Leu210 215 220Ala Cys Gln Ser Leu Arg Gly Gly Glu Cys Ser Met Ala Leu Ala Gly225 230 235 240Gly Val Thr Val Met Ser Ser Pro Glu Thr Phe Ile Gly Thr Gly Arg245 250 255Gly Ile Gly Leu Pro Ala Ala Ala Arg Cys Arg Ser Phe Ala Asp Gly260 265 270Ala Glu Gly Ile Ala Phe Ala Glu Gly Ala Gly Val Val Leu Leu Glu275 280 285Arg Leu Ser Thr Ala Arg Ala His Gly Arg Pro Val Leu Ala Val Val290 295 300Arg Gly Ser Ala Ile Gly Gln Glu Gly Thr Asn Asn Gly Val Ser Ala305 310 315 320Ser Asn Gly Pro Ala Gln Gln Arg Leu Ile Arg Gln Ala Leu Ala Ala325 330 335Ala Gly Leu Leu Pro His Glu Ile Asp Ala Val Glu Gly Gln Gly Thr340 345 350Gly Gly Leu Leu Ser Asp Ala Val Glu Ala Gln Ala Leu Ala Ser Val355 360 365Tyr Gly Glu Gly Arg Pro Ala Asp Arg Pro Leu Leu Leu Gly Ala Val370 375 380Lys Ser Asn Leu Gly His Thr Gln Gly Ala Ser Gly Val Ala Gly Val385 390 395 400Ile Lys Thr Val Gln Ala Met Arg His Gly Val Leu Pro Arg Thr Leu405 410 415His Thr Glu Val Pro Ser Pro His Ile Ser Trp Lys Arg Gly Arg Ile420 425 430Arg Leu Leu Thr Ala Ala Thr Pro Trp Pro Gly Thr Asp Arg Pro Leu435 440 445Arg Ser Gly Val Ser Ala Phe Gly Phe Gly Gly Thr Asn Ala His Val450 455 460Ile Val Glu Gln Ala Pro Pro Glu Asp Asp Pro Pro Pro Pro Leu Pro465 470 475 480Glu Ala Glu Gly Glu Pro Gly Ser Ala Gly Val Ala Leu Trp Pro Leu485 490 495Ser Gly Cys Asp Pro Asp Ala Leu Arg Asp Gln Ala Ala Arg Leu Leu500 505 510Ala His Leu Asp Glu Arg Pro Gly Leu Arg Pro Ala Asp Val Gly Leu515 520 525Ser Leu Gly Thr Ser Arg Ala Ala Leu Glu His Arg Gly Val Val Val530 535 540Gly Glu Ser Arg Gln Glu Leu Leu Asp Gly Leu Arg Ala Leu Ala Glu545 550 555 560Gly Arg Ala Ala Pro His Val Ala Arg Gly Ala Ile Gly Arg Arg Pro565 570 575Arg Leu Val Val Leu Phe Thr Gly His Ala Pro Ala Pro Gly Thr Gly580 585 590Lys Gln Leu Tyr Asp Ala Phe Pro Ala Phe Ala Asp Ala Phe Asp Thr595 600 605Val Cys Ala Ala Leu Asp Ala His Leu Gly Phe Pro Ala Arg Asp Ala610 615 620Met Leu Thr Gly Ala Asp Pro Thr Ser Ala Ala Val Pro Asp Pro Ala625 630 635 640Arg Ala Phe Ala Ile Gln Val Ala Leu Phe Arg Leu Val Glu Ser Trp645 650 655Gly Thr Arg Pro Gly Ala Val Arg Gly His Gly Ile Gly Arg Leu Ala660 665 670Glu Asp His Val Ser Gly Arg Leu Ala Leu Pro Asp Ala Cys Ala Ala675 680 685Leu Thr Gly Pro His Glu Ala Pro Asp Pro Pro Arg Asp Phe Ala Gln690 695 700Arg Val Arg Asp Leu Ala Gly Asp Gly Thr Val Tyr Leu Glu Leu Gly705 710 715 720Gly His Glu Val Ala Gly Leu Leu Asp Gly Ala Gly Ala His Ala Val725 730 735Ala Ala Leu Arg Pro Gly Val Ala Glu Ala Thr Ser Ala Ala Thr Gly740 745 750Leu Ala Arg Ala His Ala Arg Gly Thr Ala Val Asp Trp Arg Ala Val755 760 765Phe Gly Pro Glu Ala Arg Arg Val Glu Leu Pro Thr Tyr Ala Phe Arg770 775 780Arg Gly Arg Tyr Trp Arg Ala Ala Phe Asp Met Ser Leu Val Arg Pro785 790 795 800Asp Arg<210> 27<211> 2187<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Ala Asn Gln Glu Gln Leu Val Asp Tyr Leu Lys Arg Val Ala Thr1 5 10 15Asp Leu His Asp Thr Gln Gln Arg Leu Arg Glu Val Glu Ala Arg Asp20 25 30Arg Gln Pro Ile Ala Ile Val Gly Met Ala Cys Arg Phe Pro Gly Gly35 40 45Thr Asp Thr Pro Glu Ala Leu Trp Asp Leu Val Ser Glu Gly Arg Asp50 55 60Val Ile Ser Pro Met Pro Asp Asp Arg Gly Trp Asp Pro Gly Met Phe65 70 75 80Asn Asp Ser Gly Glu Thr Gly Thr Ser Tyr Val Arg Glu Gly Gly Phe85 90 95Val His Asp Ile Ala Asp Phe Asp Ala Asp Phe Phe Asp Ile Asn Pro100 105 110Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr115 120 125Ser Trp Glu Ala Ile Glu Arg Ala Arg Ile Asp Leu Thr Ser Leu Arg130 135 140Gly Ser Lys Thr Gly Val Phe Val Gly Gly Ser Thr Val Tyr Tyr Ala145 150 155 160Gly Asn Ala Ala Gly Val Pro Gln Asp Val Ala Gly Tyr Leu Ala Thr165 170 175Gly Leu Ala Ala Ser Ser Met Ser Gly Arg Ile Ser Tyr Thr Phe Gly180 185 190Phe Glu Gly Pro Ser Phe Thr Val Asp Thr Ala Cys Ser Ser Ser Gly195 200 205Val Ala Leu His Leu Ala Val Gln Ala Leu Arg Lys Gly Glu Cys Ser210 215 220Leu Ala Leu Ala Gly Gly Val Cys Val Met Ala Thr Pro Gly Thr Tyr225 230 235 240Leu Glu Phe Ser Lys Leu Asn Gly Leu Ala Ala Asp Gly Arg Cys Lys245 250 255Ala Phe Ala Ala Ala Ala Asp Gly Phe Gly Pro Ala Glu Gly Val Gly260 265 270Val Leu Leu Val Glu Arg Leu Ala Asp Ala Glu Arg Leu Gly His Pro275 280 285Val Leu Ala Val Ile Arg Gly Ser Ala Ile Asn Gln Asp Gly Ala Ser290 295 300Asn Gly Leu Thr Ala Pro His Gly Pro Ala Gln Glu Arg Val Ile Arg305 310 315 320Ala Ala Leu Ala Asp Ala Gln Leu Ser Ala Arg Asp Ile Asp Val Val325 330 335Glu Ala His Gly Thr Gly Thr Ser Leu Gly Asp Pro Ile Glu Ala Gln340 345 350Ala Leu Ile Ala Ala Tyr Gly Arg Arg Arg Ala Asp Gly Gly Pro Leu355 360 365Trp Leu Gly Ser Val Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala370 375 380Gly Met Ala Gly Val Ile Lys Met Val His Ala Leu Arg His Gly Leu385 390 395 400Leu Pro Arg Thr Leu His Val Asp Glu Pro Thr His Gln Val Asp Trp405 410 415Ser Glu Gly Thr Val Arg Leu Leu Thr Glu Ala Arg Pro Trp Pro Glu420 425 430Ala Gly Gly Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Met Ser Gly435 440 445Thr Asn Thr His Val Ile Leu Glu Gln Ala Pro Pro Ala Glu Glu Pro450 455 460Ala Pro Ala Ala Glu Ser Pro Val Val Pro Trp Leu Val Ser Gly Arg465 470 475 480Gly Glu Ala Ala Leu Arg Ala Gln Ala Ala Arg Leu Arg Asp Phe Leu485 490 495Ala Glu Arg Pro Glu Ala Ser Pro Thr Arg Val Gly Phe Ala Leu Ala
500 505 510Gly Ser Arg Ala Ala Gln Ser His Arg Ala Ala Val Val Ala Ala Asp515 520 525Arg Asp Thr Leu Leu Ala Gly Leu Gly Ser Leu Ala Glu Gly Thr Pro530 535 540Ala Gly His Val Val Thr Gly Ser Val Ser Pro Gly Ala Thr Ala Phe545 550 555 560Val Phe Pro Gly Gln Gly Ser Gln Trp Val Gly Met Ala Leu Ala Leu565 570 575Ala Asp Ala Ser Pro Val Phe Ala Glu His Phe Arg Arg Cys Ala Glu580 585 590Ala Val Glu Arg His Thr Asp Tyr Thr Val Glu Ser Val Leu Arg Ala595 600 605Asp Pro Gly Ala Pro Ser Leu Asp Arg Val Asp Val Val Gln Pro Val610 615 620Leu Trp Ala Val Met Val Ala Leu Ala Glu Leu Trp Arg Ser His Gly625 630 635 640Val Glu Pro Ala Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala645 650 655Ala Cys Val Ala Gly Val Leu Ser Val Asp Asp Ala Ala Arg Val Val660 665 670Val Leu Arg Ser Gln Val Leu Pro Glu Leu Ser Gly Arg Gly Gly Met675 680 685Ala Ser Val Ala Gln Pro Val Gly Leu Val Glu Lys His Leu Glu Arg690 695 700Trp Asp Gly Arg Leu Ser Val Ala Ala Val Asn Gly Pro Ser Ser Thr705 710 715 720Val Val Ser Gly Asp Ala Asp Ala Leu Glu Glu Leu Leu Glu Gly Tyr725 730 735Glu Ala Asp Gly Val Arg Ala Arg Arg Val Pro Val Asp Tyr Ala Ser740 745 750His Cys Ala His Val Asp Ala Leu Arg Gly Pro Leu Leu Asp Ala Leu755 760 765Ser Gly Ile Glu Pro Lys Ala Gly Thr Val Pro Leu Tyr Ser Thr Val770 775 780Thr Gly Arg Arg Ile Asp Gly Thr Thr Met Asp Ala Gly Tyr Trp Tyr785 790 795 800Thr Asn Leu Arg Gln Gln Val Arg Phe Gln Glu Ala Thr Glu Ala Leu805 810 815Leu Ala Asp Gly His Gly Val Phe Ile Glu Cys Ser Pro His Pro Val820 825 830Leu Thr Ile Gly Val Gln Glu Thr Met Asp Gln Ser Gly Ala Asn Ala835 840 845Thr Ala Leu Gly Thr Leu Arg Arg Asp Glu Gly Gly Trp Asp Arg Phe850 855 860Leu Leu Ala Leu Gly Gln Ala His Thr His Gly Val Ala Val Asp Trp865 870 875 880Ser Arg Val Phe Pro Asp Gly Thr Ser Pro Ala Asp Leu Pro Thr Tyr885 890 895Ala Phe Gln Arg Arg Arg Tyr Trp Leu Asp Ala Thr Ala Gly Ala Ala900 905 910Gly Asp Pro Ala Ser Leu Gly Leu Thr Pro Ala Asp His Pro Leu Leu915 920 925Gly Ala Val Thr Leu Leu Ala Glu Gly Asp Glu Val Val Met Thr Gly930 935 940Arg Leu Gly Leu Asp Thr His Pro Trp Leu Ala Asp His Ala Val Ala945 950 955 960Gly Ala Val Leu Val Pro Gly Ala Val Phe Val Glu Leu Ala Val Arg965 970 975Ala Gly Asp Glu Val Gly Cys Asp His Leu Glu Glu Met Val Leu Ala980 985 990Ser Pro Leu Val Leu Pro Glu Gln Gly Gly Phe Asp Leu Gln Leu Val995 1000 1005Val Gly Gly Ala Ala Glu Asp Gly Arg Arg Thr Leu Gly Gly Tyr1010 1015 1020Ala Arg Pro Ser Gly Ser Asp Arg Pro Trp Val Gln His Val Thr1025 1030 1035Gly Thr Leu Ala Pro Gly Gly Ala Ala Arg Pro Phe Asp Leu Ala1040 1045 1050Gln Trp Pro Pro Glu Gly Ala Arg Pro Val Pro Val Glu Gly Cys1055 1060 1065Tyr Asp Gln Leu Ala Glu Gly Gly Phe Arg Tyr Gly Pro Ala Phe1070 1075 1080Arg Ser Leu Arg Ala Val Tyr Arg Arg Glu Thr Glu Val Phe Ala1085 1090 1095Glu Val Val Leu Pro Glu Glu Gln Arg Gly Lys Ala Ala Ala Phe1100 1105 1110Gly Ile His Pro Ala Leu Leu Asp Gly Ala Leu His Ala Ser Gly1115 1120 1125Leu Ser Ala Val Arg Gly Asp Ser Gly Gly Arg Met Ala Leu Pro1130 1135 1140Phe Ala Trp Asn Gly Val Ser Leu Ala Ala Thr Gly Ala Glu Leu1145 1150 1155Leu Arg Val Arg Leu Ala Pro Val Gly Asp Asp Gly Met Ser Val1160 1165 1170His Ala Thr Asp Ala Ser Gly His Pro Val Ile Ser Ile Glu Ser
1175 1180 1185Leu Val Thr Arg Pro Phe Thr Ala Gly Gln Leu Pro Ser Asp Gly1190 1195 1200Asp Glu Thr Arg Asp Gly Leu Phe Arg Val Ala Tyr Thr Pro Leu1205 1210 1215Thr Asp Pro Glu Pro Gly Thr Ala Thr Asp Asp Trp Thr Ala Val1220 1225 1230Ala Thr Gly Ser Glu Pro Thr Tyr Tyr Gly Val Arg Arg Tyr Gly1235 1240 1245Asp Leu Asp Ala Leu Ala Ala Ala Val Asp Gly Gly Leu Pro Ala1250 1255 1260Pro Pro Val Thr LeL Leu Pro Cys Glu Pro Ala Pro Asp Asp Gly1265 1270 1275Asp Leu Pro Gly Ala Leu Arg Arg Arg Leu Gly Glu Val Leu His1280 1285 1290Thr Val Gln Arg Trp Leu Ala Asp Glu Arg Phe Ala Ala Ser Arg1295 1300 1305Leu Val Val Val Thr Arg Gly Ala Val Ala Ala Phe Glu Gly Asp1310 1315 1320Asp Val Thr Asp Leu Val His Ala Pro Val Trp Gly Leu Ile Arg1325 1330 1335Ser Ala Gln Ser Glu His Pro Asp Arg Leu Ala Leu Val Asp Leu1340 1345 1350Asp Gly Pro Glu Leu Pro Lys Ala Val Ala Ala Ala Val Ala Val1355 1360 1365Ala Val Ala Ala Gly Glu Lys Gln Ile Val Val Arg Asp Gly Thr1370 1375 1380Val Arg Val Ser Arg Leu Val Pro Ala Val Ser Gly Gly Ala Leu1385 1390 1395Ala Leu Pro Glu Thr Pro His Trp Arg Leu Asp Ile Ala Ala Pro1400 1405 1410Ala Thr Leu Asp Asn Leu Gly Leu Val Ala Ala Glu Glu Pro Gly1415 1420 1425Pro Pro Ala Pro Gly His Val Arg Val Gln Val Arg Ala Ala Gly1430 1435 1440Val Asn Phe Arg Asp Val Leu Ile Ala Leu Gly Met Tyr Pro Gly1445 1450 1455Asp Gly Ala Phe Arg Gly Ser Glu Gly Ala Gly Val Val Leu Glu1460 1465 1470Val Ala Glu Asp Val Thr Ser Val Ala Val Gly Asp Arg Val Met1475 1480 1485Gly Met Phe Gln Gly Ala Phe Gly Ser Thr Ala Val Ala Asp Ala1490 1495 1500Arg Ser Val Val Pro Ile Pro Pro Gly Trp Thr Asp Glu Gln Ala1505 1510 1515Ala Ala Val Pro Ile Ala Tyr Val Thr Ala Trp Tyr Gly Leu Val1520 1525 1530Asp Leu Ala Gly Leu Lys Ala Gly Glu Ser Val Leu Ile His Ala1535 1540 1545Ala Thr Gly Gly Val Gly Thr Ala Ala Val Gln Ile Ala Arg His1550 1555 1560Leu Gly Ala Glu Val Tyr Ala Thr Ala Gly Pro Gly Lys His His1565 1570 1575Val Leu Glu Ala Met Gly Ile Asp Glu Ala His Arg Ala Ser Ser1580 1585 1590Arg Asp Leu Asp Phe Glu Asp Ala Phe Arg Ala Ala Thr Gly Gly1595 1600 1605Arg Gly Val Asp Val Val Leu Asn Ser Leu Thr Gly Asp His Thr1610 1615 1620Asp Ala Ser Leu Arg Leu Leu Ala Glu Lys Gly Arg Phe Val Glu1625 1630 1635Leu Gly Met Thr Asp Val Arg Asp Pro Glu Gln Leu Ala Glu Pro1640 1645 1650Tyr Pro Gly Leu Arg Tyr Arg Val Leu Asp Leu Arg Glu Pro Gly1655 1660 1665Glu Asp Gly Ile Gly Arg Met Leu Thr Glu Ile Leu Gly Arg Phe1670 1675 1680Ala Gly Gly Glu Leu Thr His Pro Ala Val Arg Ala Phe Asp Ile1685 1690 1695Arg Arg Ala Arg Asp Ala Phe Arg Leu Met Ser Arg Ala Ala His1700 1705 1710Ile Gly Lys Ile Val Leu Thr Leu Pro Arg Pro Leu Asp Pro Asp1715 1720 1725Gly Thr Val Leu Val Thr Gly Gly Thr Gly Thr Leu Gly Gly Leu1730 1735 1740Ile Ala Arg His Leu Val Thr Arg His Gly Val Arg His Leu Leu1745 1750 1755Leu Thr Ser Arg Arg Gly Pro Glu Ala Pro Gly Ala Pro Ala Leu1760 1765 1770Arg Glu Asp Leu Ala Ala Leu Gly Ala Thr Val Thr Val Thr Ala1775 1780 1785Cys Asp Ala Gly Asp Arg Glu Arg Leu Ala Glu Val Leu Ala Gly1790 1795 1800Val Pro Asp Ala His Pro Leu Thr Gly Val Val His Cys Ala Gly1805 1810 1815Val Leu Asp Asp Gly Met Val Asp Ala Leu Thr Pro Glu Gln Leu
1820 1825 1830Ala Arg Val Leu Arg Pro Lys Ala Glu Ala Ala Leu His Leu His1835 1840 1845Glu Leu Thr Gln Asp Ala Asp Leu Ala Leu Phe Val Leu Phe Ser1850 1855 1860Ser Ile Val Gly Val Tyr Gly Asn Pro Ser Gln Ala Asn Tyr Ala1865 1870 1875Ala Ala Ser Thr Phe Leu Asp Ala Leu Ala Gln His Arg Gln Ala1880 1885 1890Gly Gly Leu Pro Ala Gln Ser Leu Ala Trp Gly Met Trp Glu Glu1895 1900 1905Thr Ser Ala Leu Thr Gly Glu Leu Asp Asp Gly Val Arg Gln Arg1910 1915 1920Ile Ser Gln Ala Gly Met Asp Pro Leu Pro Thr Glu Gln Ala Leu1925 1930 1935Ala Leu Phe Asp Arg Ser Tyr Ala Val Gly Asp Ala Leu Leu Val1940 1945 1950Pro Ile Arg Gln Ser Thr Ala Ala Ser Arg Gly Gly Pro Ala Ala1955 1960 1965Gly Arg Thr Arg Ala Arg Arg Val Ala Asp Ser Gly Val Ala Gly1970 1975 1980Thr Gly Gly Pro Ser Leu Thr Asp Arg Val Thr Ala Leu Pro Glu1985 1990 1995Ala Glu Arg Asp Ser Cys Val Leu Glu Ala Val Arg Ala His Met2000 2005 2010Ala Ala Val Leu Gly His Asp Ser Ala Asp Glu Ile Ala Pro Asp2015 2020 2025Arg Thr Phe Lys Glu Leu Gly Phe Asp Ser Leu Thr Ala Val Glu2030 2035 2040Leu Arg Asn Arg Leu Ser Ala Ala Thr Gly Leu Arg Leu Pro Ala2045 2050 2055Thr Phe Val Phe Asp Phe Ala Asn Pro Val Ala Leu Ala Glu His2060 2065 2070Leu Arg Glu Gln Ile Ala Pro Pro Thr Glu Leu Gly Pro Pro Thr2075 2080 2085Asp Pro Ser Thr Pro Ala Asp Pro Ser Pro Pro Pro Gly Leu Pro2090 2095 2100Glu Gly Pro Ser Thr Asp Pro Arg Glu Ser Arg Val Arg Gln Val2105 2110 2115Leu Ala Ala Ile Pro Leu Arg Arg Leu Glu Glu Ser Gly Leu Leu2120 2125 2130Glu Thr Leu Leu Arg Leu Gly Glu Gly Pro Gly Glu Ala Asp Thr2135 2140 2145Thr Gly Ala Arg Pro Val Glu Gly Thr Arg Asp Glu Thr His Thr2150 2155 2160Pro Asp Ser Ala Asp Ile Ala Ser Met Asp Leu Glu Glu Leu Val2165 2170 2175Asn Ala Ala Leu Arg Asn Asp Glu Ser2180 2185<210> 28<211> 3455<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Ile Leu Ser Arg Pro Arg Glu Glu Glu Ala Pro Ala Met Ala Lys1 5 10 15Asp Glu Ala Lys Leu Leu Asp His Leu Lys Trp Val Thr Ala Glu Leu20 25 30Arg Asp Thr Arg Arg Arg Leu Arg Glu Ala Glu Ser Thr Glu Pro Glu35 40 45Pro Ile Ala Ile Val Gly Met Ala Cys Arg Phe Pro Gly Gly Val Asp50 55 60Asn Pro Asp Asp Leu Trp Gln Leu Val Ala Ala Gly Gly Glu Gly Leu65 70 75 80Thr Glu Phe Pro Glu Asp Arg Gly Trp Asp Leu Glu Asn Leu Phe Asp85 90 95Pro Asp Pro Asp Ser Ala Gly Thr Ser Tyr Val Arg Arg Gly Ala Phe100 105 110Leu Ser Gly Ala Gly Gly Phe Asp Ala Glu Phe Phe Gly Ile Ser Pro115 120 125Arg Glu Ala Val Ala Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Val130 135 140Ala Trp Glu Thr Phe Glu His Ala Gly Ile Asp Pro His Gly Leu Val145 150 155 160Gly Ser Ser Thr Gly Val Tyr Ala Gly Val Thr Ser Gln Glu Tyr Met165 170 175Met Leu Thr Ala Met Ala Gly Ser Asp Val Glu Gly Tyr Ala Ala Thr180 185 190Gly Asn Leu Ala Cys Val Leu Ser Gly Arg Val Ser Tyr Val Leu Gly195 200 205Leu Glu Gly Pro Ala Val Thr Val Asp Thr Gly Cys Ser Ser Ser Leu210 215 220Val Ala Leu His Ser Ala Val Gln Ala Leu Arg Gly Gly Glu Cys Ser225 230 235 240Leu Ala Leu Ala Gly Gly Val Thr Val Met Ala Thr Pro Gly Ala Phe245 250 255Val Glu Phe Ser Arg Gln Arg Gly Leu Ala Ala Asp Gly Arg Cys Lys260 265 270Ala Phe Ala Gly Ala Ala Asp Gly Thr Gly Trp Gly Glu Gly Val Gly275 280 285Leu Leu Leu Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Lys290 295 300Val Trp Ala Val Val Arg Gly Ser Ala Ile Asn Gln Asp Gly Ala Ser305 310 315 320Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg325 330 335Gln Ala Leu Ala Asn Ala Arg Leu Ser Pro Ala Asp Val Asp Val Val340 345 350Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln355 360 365Ala Leu Leu Ala Thr Tyr Gly Gln Gly Arg Ser Ala Glu Arg Pro Leu370 375 380Trp Leu Gly Ser Ile Lys Ser Asn Leu Ala His Thr Gln Ala Ala Ala385 390 395 400Gly Val Ala Gly Val Ile Lys Met Val Met Ala Met Arg His Gly Leu405 410 415Leu Pro Gln Thr Leu His Val Asp Ala Pro Thr Pro His Val Asp Trp420 425 430Asp Ser Gly Ala Val Ala Leu Leu Ser Glu Ala Thr Asp Trp Pro Glu
435 440 445Val Asp Arg Pro Arg Arg Ala Gly Val Ser Ala Phe Gly Ile Ser Gly450 455 460Thr Asn Ala His Val Ile Leu Glu Glu Ala Pro Ala Leu Ser Thr Glu465 470 475 480Gly Glu Asn Ala Glu Pro Thr Thr Pro Gly Gly Val Val Pro Trp Val485 490 495Val Ser Gly Arg Ser Ala Ala Gly Leu Arg Ala Gln Ala Gly Arg Leu500 505 510Ala Glu Phe Ala Glu Gln Ala Thr Ala Asp Val Ala Glu Val Gly Trp515 520 525Ser Leu Val Ala Gly Arg Ala Met Leu Glu His Arg Ala Val Val Val530 535 540Gly Asp Asp Arg Asn Glu Leu Leu Ala Gly Leu Arg Ala Leu Ala Glu545 550 555 560Gly Val Pro Phe Gly Gly Val Val Ser Val Asp Pro Val Ala Gly Gly565 570 575Ala Gly Pro Val Leu Val Phe Pro Gly Gln Gly Gly Gln Trp Arg Gly580 585 590Met Gly Val Glu Leu Leu Asp Ala Ser Pro Val Phe Ala Gly Arg Ile595 600 605Ala Glu Cys Glu Val Ala Leu Ala Pro Phe Val Glu Trp Ser Leu Thr610 615 620Ala Val Leu Arg Gly Glu Asp Gly Val Asp Val Ser Arg Val Asp Val625 630 635 640Val Gln Pro Ala Leu Trp Ala Val Met Val Ser Leu Ala Glu Val Trp645 650 655Arg Ser Tyr Gly Val Glu Pro Ala Ala Val Val Gly His Ser Gln Gly660 665 670Glu Ile Ala Ala Ala Val Val Ala Gly Ala Leu Thr Leu Arg Asp Gly675 680 685Ala Arg Val Val Ala Leu Arg Ser Arg Ala Leu Arg Val Leu Ser Gly690 695 700Arg Gly Ala Met Ala Ser Leu Ala Val Gly Arg Glu Glu Ala Glu Lys705 710 715 720Ala Ile Gly Gly Arg Ala Gly Val Val Val Ala Ala Val Asn Gly Pro725 730 735Gly Ser Thr Val Val Ser Gly Pro Pro Val Ala Val Ala Glu Val Val740 745 750Ala Ala Val Glu Ala Ala Gly Gly Arg Ala Arg Leu Val Asp Val Asp755 760 765Tyr Ala Ser His Asn Pro Gln Val Asp Asp Ile Ala Asp Glu Leu Ile770 775 780Glu Val Leu Gly Gln Val Thr Pro Val Glu Thr Ala Val Ala Phe Tyr785 790 795 800Ser Ala Val Thr Gly Gly Arg Val Glu Ser Thr Ala Leu Asp Ala Ala805 810 815Tyr Trp Val Glu Asn Leu Arg Arg Gln Val Arg Phe Ala Thr Ala Val820 825 830Glu Ala Leu Leu Ser Asp Gly Tyr Arg Val Leu Val Glu Ser Ser Pro835 840 845His Pro Val Leu Ser Val Gly Val Gln Glu Thr Ala Gln Ala Leu Asp850 855 860Val Pro Val Ala Thr Val Ala Thr Leu Gln Arg Asp Gln Gly Gly Ala865 870 875 880Val Gln Leu Ala Arg Ala Leu Ala Gln Ala Phe Thr Ala Gly Leu Ser885 890 895Val Asp Trp Lys Ala Trp Tyr Gly Val Asp Thr Thr Pro Asp Val Pro900 905 910Thr Gly Ala Ile Thr Pro Ala Pro Pro Val Leu Asp Leu Pro Thr Tyr915 920 925Ala Phe Gln His Gln His Tyr Trp Leu Lys Pro Gly Arg Trp Gly Ser930 935 940Ser Pro Glu Gln Gly Ser Glu Asp Asp Glu Arg Phe Trp Ser Ala Val945 950 955 960Glu Ala Gly Asp Leu Ala Gly Leu Gly Ala Ser Leu Asn Val Ala Glu965 970 975Asp Thr Ala Arg Gln Ala Leu Ala Pro Ala Leu Pro Val Leu Ala Glu980 985 990Trp Arg Gln Arg Thr Arg Asp Arg Ala Arg Ile Asp Gly Trp Arg Tyr995 1000 1005Arg Thr Ala Trp Ser Thr Val Thr Gly Leu Asp Ala Ala Pro Arg1010 1015 1020Leu Ser Gly Thr Trp Leu Leu Val Val Pro Glu Lys Leu Glu Gly1025 1030 1035Asp Ala Ala Val Glu Thr Val Val Arg Ala Leu Glu Gly Arg Gly1040 1045 1050Ala Arg Cys Thr Thr Leu Ala Leu Ala Pro Gly Asp Gln Ala Arg1055 1060 1065Asp Pro Leu Thr Thr Arg Leu Arg Gln Leu Glu Gly Gly Pro Glu1070 1075 1080Val Ala Gly Val Ile Ser Ala Leu Gly Leu Asp Glu Ala Val His1085 1090 1095Pro Asp His Pro His Leu Ser Val Gly Leu Ala Gly Thr Ile Ala1100 1105 1110Leu Val Gln Ala Leu Asp Glu Met Asp Phe Gly Gly Arg Leu Trp
1115 1120 1125Cys Val Thr Arg Gly Gly Val Ser Val Gly Glu Asp Gly Pro Val1130 1135 1140Ser Pro Ala Gln Ala Gln Val Trp Gly Leu Gly Arg Val Met Ala1145 1150 1155Leu Glu Tyr Pro Lys Arg Trp Gly Gly Leu Val Asp Leu Pro Ala1160 1165 1170Ala Ala Glu Glu Glu Thr Ala Ser Arg Leu Ala Ala Val Val Ala1175 1180 1185Glu Gly Thr Glu Asp Gln Val Ala Leu Arg Ala Asp Gly Ala Leu1190 1195 1200Gly Arg Arg Leu Arg Ala Ala Pro Gly Gly Ala Pro Gly Glu Glu1205 1210 1215Trp Arg Thr Glu Gly Ser Val Leu Val Thr Gly Gly Thr Gly Gly1220 1225 1230Val Gly Gly Arg Val Ala Arg Trp Val Val Glu Arg Gly Ala Arg1235 1240 1245His Val Ile Val Ala Gly Arg Arg Gly Pro Ala Ala Pro Gly Ala1250 1255 1260Glu Glu Leu Thr Ala Glu Leu Glu Ala Leu Gly Ala Ser Val Asp1265 1270 1275Val Val Ala Cys Asp Val Ala Asp Arg Asp Gln Ala Ala Gly Leu1280 1285 1290Leu Ala Arg Val Pro Glu Glu His Pro Leu Arg Gly Ile Phe His1295 1300 1305Ala Ala Gly Val Gly Asp Tyr Thr Pro Val Arg Asp Leu Asp Pro1310 1315 1320Tyr Arg Val Ala Gln Val Thr Ala Ala Lys Ala Gly Gly Ala Arg1325 1330 1335Trp Leu Asp Glu Leu Thr Arg Asp Leu Asp Val Ser Ala Phe Val1340 1345 1350Leu Phe Ser Ser Gly Ala Ala Ser Trp Gly Ser Gly Gln Gln Gly1355 1360 1365Ala Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Ala Leu Ala Glu Arg1370 1375 1380Arg Arg Ala Glu Gly Leu Pro Gly Leu Ser Val Ala Trp Gly Pro1385 1390 1395Trp Gly Glu Ala Gly Met Ala Ala Asp Gln Ala Val Ala Thr Phe1400 1405 1410Phe Arg Asp Arg Gly Leu Thr Ala Met Pro Pro Glu Leu Ala Leu1415 1420 1425Arg Val Leu Gly Asp Ala Leu Gly Arg Gly Glu Thr Thr Leu Thr1430 1435 1440Val Ala Asp Phe Asp Trp Ala Arg Phe Ala Ala Thr Phe Ala Gly1445 1450 1455Gln Arg Ala Ser Arg Leu Leu Ala Glu Ile Pro Gln Ala Val Glu1460 1465 1470Leu Leu Glu Lys Glu Thr Pro Ser Glu Asp Ser Pro Leu Arg Arg1475 1480 1485Gln Leu Ser Ala Ala Ala Pro Glu Gln Arg His Gln Ile Leu Ser1490 1495 1500Gln His Ile Arg Ala Leu Ala Ala Gly Val Leu Gly His Ser Gly1505 1510 1515Pro Asp Ala Val Ser Ala Thr Lys Pro Phe Phe Glu Met Gly Phe1520 1525 1530Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Lys Leu Ser Ser Ser1535 1540 1545Ala Gly Met Pro Leu Pro Thr Thr Leu Ile Phe Asp Tyr Pro Thr1550 1555 1560Ala Asp Asp Leu Ala Arg His Val Leu Gly Glu Ile Ser Gly Thr1565 1570 1575Gln Thr Ala Val Ala Asp Ala Thr Val Val Pro Val Ala Gly Pro1580 1585 1590Ile Gly Glu Pro Asp Glu Pro Ile Ala Ile Val Gly Met Ala Cys1595 1600 1605Arg Phe Pro Gly Gly Val Thr Ser Pro Glu Gln Leu Trp Asp Leu1610 1615 1620Val Thr Glu Gly Arg Asp Ala Met Ser Ala Phe Pro Thr Asp Arg1625 1630 1635Gly Trp Arg Leu Asp Thr Leu Tyr Asp Pro Asp Pro Asp Arg Pro1640 1645 1650Gly Thr Ser Tyr Val Arg Glu Gly Gly Phe Ile Tyr Asp Ala Gly1655 1660 1665Glu Phe Asp Ala Asp Phe Phe Gly Ile Ser Pro Arg Glu Ala Val1670 1675 1680Gly Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ala Trp Glu1685 1690 1695Thr Phe Glu Arg Ala Gly Ile Asp His Gly Ser Leu Arg Gly Ser1700 1705 1710Asp Thr Gly Val Tyr Val Gly Ala Thr Ile Phe Asp Tyr Leu Ser1715 1720 1725Ile Ile Gly Ile Ser Ser Leu Asp Met Glu Gly Tyr Thr Gly Thr1730 1735 1740Gly Asn Leu Gly Cys Val Val Ser Gly Arg Val Ser Tyr Val Leu1745 1750 1755Gly Leu Glu Gly Pro Ala Val Thr Val Asp Thr Gly Cys Ser Ser
1760 1765 1770Ser Leu Val Ala Leu His Ser Ala Val Arg Gly Leu Arg Gly Gly1775 1780 1785Glu Cys Ser Leu Ala Leu Ala Gly Gly Val Thr Val Met Ser Thr1790 1795 1800Pro Gly Ala Phe Val Glu Phe Ser Arg Gln Arg Gly Leu Ala Ala1805 1810 1815Asp Gly Arg Cys Lys Ala Phe Ala Ala Ala Ala Asp Gly Thr Gly1820 1825 1830Trp Gly Glu Gly Val Gly Leu Leu Leu Leu Glu Arg Leu Ser Asp1835 1840 1845Ala Arg Arg Asn Gly His Lys Val Trp Ala Val Val Arg Gly Ser1850 1855 1860Ala Ile Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn1865 1870 1875Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Asn Ala1880 1885 1890Arg Leu Ser Pro Ala Asp Val Asp Val Val Glu Ala His Gly Thr1895 1900 1905Gly Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala1910 1915 1920Thr Tyr Gly Gln Gly Arg Ser Ala Glu Arg Pro Leu Trp Leu Gly1925 1930 1935Ser Val Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly Ala1940 1945 1950Ala Gly Val Ile Lys Met Val Met Ala Met Arg His Gly Glu Leu1955 1960 1965Pro Ala Ser Leu Phe Ile Asp Ala Pro Thr Pro His Val Asp Trp1970 1975 1980Asp Ser Gly Ala Val Ala Leu Leu Ser Glu Ala Thr Asp Trp Pro1985 1990 1995Glu Val Asp Arg Pro Trp Arg Ala Gly Val Ser Ala Phe Gly Ile2000 2005 2010Ser Gly Thr Asn Ala His Val Ile Leu Glu Glu Ala Pro Glu Asp2015 2020 2025Pro Tyr Val Asp Ala Pro Val Asp Cys Gly Pro Ala Pro Val Gly2030 2035 2040Gly Val Val Pro Trp Val Val Ser Gly Arg Ser Ala Ala Gly Leu2045 2050 2055Arg Ala Gln Ala Gly Arg Leu Ala Glu Phe Val Leu Glu Thr Val2060 2065 2070Asp Glu Val Ala Glu Val Gly Trp Ser Leu Val Ala Gly Arg Ala2075 2080 2085Val His Asp His Arg Ala Val Val Val Gly Gln Asp Arg Thr Glu2090 2095 2100Leu Leu Thr Gly Leu Thr Ala Leu Ala Asp Gly Leu Pro Ser Gly2105 2110 2115Gly Val Val Ala Gly Glu Pro Val Val Gly Gly Ala Gly Pro Val2120 2125 2130Leu Val Phe Pro Gly Gln Gly Gly Gln Trp Arg Gly Met Gly Val2135 2140 2145Glu Leu Leu Asp Ala Ser Pro Val Phe Ala Gly Arg Ile Ala Glu2150 2155 2160Cys Glu Val Ala Leu Ala Pro Phe Val Glu Trp Ser Leu Thr Ala2165 2170 2175Val Leu Arg Gly Glu Asp Gly Val Asp Val Ser Arg Val Asp Val2180 2185 2190Val Gln Pro Ala Leu Trp Ala Val Met Val Ser Leu Ala Glu Val2195 2200 2205Trp Arg Ser Tyr Gly Val Glu Pro Ala Ala Val Val Gly His Ser2210 2215 2220Gln Gly Glu Ile Ala Ala Ala Val Val Ala Gly Ala Leu Thr Leu2225 2230 2235Arg Asp Gly Ala Arg Val Val Ala Leu Arg Ser Arg Ala Leu Arg2240 2245 2250Val Leu Ser Gly Arg Gly Ala Met Ala Ser Leu Ala Val Gly Arg2255 2260 2265Glu Glu Ala Glu Lys Ala Ile Gly Gly Arg Ala Gly Val Val Val2270 2275 2280Ala Ala Val Asn Gly Pro Gly Ser Thr Val Val Ser Gly Pro Pro2285 2290 2295Val Ala Val Ala Glu Val Val Ala Ala Val Glu Ala Ala Gly Gly2300 2305 2310Arg Ala Arg Leu Val Asp Val Asp Tyr Ala Ser His Asn Pro Gln2315 2320 2325Val Asp Asp Ile Ala Asp Glu Leu Ile Glu Val Leu Gly Gln Val2330 2335 2340Thr Pro Val Glu Thr Ala Val Ala Phe Tyr Ser Ala Val Thr Gly2345 2350 2355Gly Arg Val Glu Ser Thr Ala Leu Asp Ala Ala Tyr Trp Val Glu2360 2365 2370Asn Leu Arg Arg Gln Val Arg Phe Ala Thr Ala Val Glu Ala Leu2375 2380 2385Leu Ser Asp Gly Tyr Arg Val Leu Val Glu Ser Ser Pro His Pro2390 2395 2400Val Leu Ser Val Gly Ile Gln Glu Thr Ala Gln Ala Leu Asp Val
2405 2410 2415Pro Val Ala Thr Val Ala Thr Leu Gln Arg Asp Gln Gly Gly Pro2420 2425 2430Val Gln Leu Ala Arg Ala Leu Ala Gln Ala Phe Thr Ala Gly Leu2435 2440 2445Ser Val Asn Trp Lys Ala Trp Tyr Gly Val Asp Thr Thr Pro Asp2450 2455 2460Val Pro Thr Gly Val Thr Thr Pro Ala Pro Pro Val Leu Asp Leu2465 2470 2475Pro Thr Tyr Ala Phe Gln His Gln His Tyr Trp Ile Asp Ala Ser2480 2485 2490Thr Ile Gly Gly Gln Gly Asp Pro His Ser Leu Gly Leu Ala Ser2495 2500 2505Ala Asp His Pro Leu Leu Gly Ala Ala Val Arg Val Ala Glu Ser2510 2515 2520Glu Glu Val Leu Leu Thr Gly Arg Ile Ser Thr Ala Gly His Ser2525 2530 2535Trp Leu Ala Asp His Ala Val Ala Gly Ser Val Phe Leu Pro Gly2540 2545 2550Thr Ala Phe Val Asp Leu Ala Val Arg Ala Gly Asp Glu Val Gly2555 2560 2565Cys Asp Arg Val Glu Glu Leu Val Leu Glu Thr Pro Leu Val Leu2570 2575 2580Pro Glu Asp Gly Ala Leu Gln Leu Gln Leu Val Val Ser Ala Pro2585 2590 2595Arg Gln Asp Gly Gly Arg Gly Phe Thr Val His Ala Arg Ala Asp2600 2605 2610Gly Pro Asp Asp Ala Asp Arg Pro Trp Thr Arg His Ala Ser Gly2615 2620 2625Thr Leu Thr Thr Gly Ala Arg Pro Asp Asp Phe Asp Phe Thr Gln2630 2635 2640Trp Pro Pro Ala Gly Ala Glu Pro Leu Ser Leu Asp Gly Leu Tyr2645 2650 2655Ala Gly Leu Ala Glu Ala Gly Tyr Gly Tyr Gly Pro Ala Phe Arg2660 2665 2670Gly Leu Lys Ala Ala Trp Arg Arg Gly Asp Glu Val Phe Ala Glu2675 2680 2685Ala Ala Leu Ala Glu Glu Leu His Gly Gly Ala Gly Arg Phe Gly2690 2695 2700Leu His Pro Ala Leu Leu Asp Thr Ala Leu Gln Ala Gly Gly Ile2705 2710 2715Gly Gln Gly Gly Pro Pro Asp Gly Gln Met Leu Leu Pro Phe Thr2720 2725 2730Trp Asn Gly Val Ser Leu Tyr Ala Thr Gly Ala Ser Ala Leu Arg2735 2740 2745Val Arg Met Val Pro Asn Glu Thr Ala Asp Gly Val Ser Leu Ser2750 2755 2760Val Ala Asp Pro Ser Gly Arg Gln Val Ala Ser Ala Asp Ala Val2765 2770 2775Val Phe Arg Pro Val Ser Leu Glu Gln Leu Ser Arg Gly Gly Ser2780 2785 2790Glu Leu Asp Ser Leu Tyr Arg Val Glu Trp Ile Ser Ala Glu Pro2795 2800 2805Arg Pro Glu Val Leu Ala Asp Arg Ala Trp Glu Val Val Gly Ala2810 2815 2820Asp Gly Leu Gly Ile Thr Asn Ala Leu Glu Arg Ala Gly His Ala2825 2830 2835Val Arg Thr Thr Thr Asp Leu Ala Glu Ser Ala Arg Tyr Val Asp2840 2845 2850Asp Asp Ala Pro Val Ser Asp Leu Met Val Leu Asp Cys Ala Pro2855 2860 2865Leu Ala Gly Gly Ala Thr Ala Leu Asp Pro Gly Leu Ala Ala Ala2870 2875 2880Val His Asp Glu Thr Ala Arg Leu Leu Gly Val Val Gln Arg Trp2885 2890 2895Leu Ala Asp Gly Arg Phe Ala Asp Ser Arg Leu Val Leu Val Thr2900 2905 2910Arg Gly Ala Gln Ser Thr Ser Gly Asp Glu Gly Val Thr Asp Leu2915 2920 2925Val His Ser Ala Leu Trp Gly Leu Val Arg Ser Ala Gln Leu Glu2930 2935 2940Asn Ile Asp Arg Phe Val Leu Val Asp Leu Asp Gly Ala Asp Pro2945 2950 2955Asp Ala Ala Ala Leu Leu Pro Gly Ala Leu Ser Val Ala Leu Gly2960 2965 2970Ala Gly Glu Pro Gln Val Ala Val Arg Gln Gly Arg Val Val Val2975 2980 2985Pro Arg Leu Ala Arg Ile Gly Gln Asp Thr Ala Pro Thr Pro Pro2990 2995 3000Asp Leu Pro Gly Arg Arg Leu Asp Ser Glu Gly Thr Val Leu Ile3005 3010 3015Thr Gly Gly Thr Gly Thr Ile Gly Gly Val Val Ala Arg His Leu3020 3025 3030Val Thr Thr Arg Gly Ala Arg His Leu Leu Leu Thr Ser Arg Arg3035 3040 3045Gly Ala Asp Ala Pro Gly Ala Ala Glu Leu Arg Asp Glu Leu Thr
3050 3055 3060Ser Leu Gly Ala Glu Val Thr Ile Ala Ala Cys Asp Ala Ala Asp3065 3070 3075Arg Asp Ala Leu Ala Glu Leu Leu Ala Asp Ile Pro Ala Ala His3080 3085 3090Pro Leu Thr Gly Val Ile His Ala Ala Gly Val Ile Asp Asp Gly3095 3100 3105Thr Ile Pro Ser Leu Thr Gly Glu Arg Leu Arg Ala Val Leu Arg3110 3115 3120Pro Lys Val Asp Ala Ala Val Asn Leu His Gln Leu Thr Arg Glu3125 3130 3135Lys Asp Leu Ala Thr Phe Val Leu Phe Ser Ser Thr Gly Gly Val3140 3145 3150Thr Gly Val Gly Gly Gln Ser Asn Tyr Thr Ala Ser Asn Ala Phe3155 3160 3165Leu Asp Ala Leu Ala Gln Asp Arg Arg Ala Ala Gly Phe Pro Gly3170 3175 3180Gln Ser Leu Ser Trp Gly Tyr Trp Glu Gln Thr Ser Gly Ile Thr3185 3190 3195Gly Thr Leu Asp Glu Arg Asp Ile Ala Arg Met Glu Arg Ser Gly3200 3205 3210Ile Arg Ala Met Ser Ser Glu Gln Gly Leu Ala Leu Phe Asp Ala3215 3220 3225Ala Glu Arg Arg Pro Glu Ser Leu Leu Val Pro Ala Arg Leu Asp3230 3235 3240Pro Glu Ala Leu Arg Asp Leu Ala Asp Ala Arg Val Leu Pro Arg3245 3250 3255Ile Leu Ser Gly Leu Val Arg Gln Ala Pro Ala Arg Arg Ala Ala3260 3265 3270Ala Ala Gly Gln Pro Ala Thr Gly Asp Asp Leu Thr Met Ala Glu3275 3280 3285Arg Leu Thr Gly Leu Ser Ala Ala Glu Gln Ser Arg Thr Leu Leu3290 3295 3300Glu Leu Val Arg Arg Asn Val Ala Ala Val Leu Gly Leu Gly Asp3305 3310 3315Val Leu Ala Val Asp Pro Ser Arg Pro Phe Lys Glu Ile Gly Phe3320 3325 3330Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Ser Arg Ala3335 3340 3345Thr Asp Thr Thr Leu Pro Ser Thr Leu Val Phe Asp Ile Pro Thr3350 3355 3360Pro Ala Leu Leu Ala Glu His Leu Arg Glu Gln Leu Val Ser Glu3365 3370 3375Gly Leu Ser Gly Ser Glu Ala Leu Ile Gln Glu Leu Asp Arg Leu3380 3385 3390Glu Asp His Val Asp Leu Leu Val Asp Asp Gly Glu Arg Asp Ala3395 3400 3405Val Thr Ala Arg Leu Glu Ala Leu Leu Thr Arg Cys Arg Gln Lys3410 3415 3420Pro Thr Ala Ala Glu Gly Asn Gly Val Ala Glu Arg Leu Gln Glu3425 3430 3435Ala Ser Ala Asp Glu Val Leu Gln Phe Ile Asp Ser His Leu Gly3440 3445 3450Arg Ala3455<210> 29<211> 423<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Leu Asn Arg Gly Val Val Ser Pro Thr Glu Ala Thr Pro Ala Ser Ser1 5 10 15Ala Lys Ala Thr Arg Pro Pro Asp Phe Met Asp Pro Ser Phe Trp Leu20 25 30Arg Pro Arg Asp Glu Arg Ala Glu Val Phe Glu Lys Leu Arg Ala Leu35 40 45Pro Gly Pro Glu Phe Val Pro Pro Arg Leu Pro Trp Gly Pro Leu Ala50 55 60Ser Gly Tyr Tyr Ala Leu Ser Lys His Ala Asp Ile Cys Glu Val Ser65 70 75 80Arg Arg Pro Gln Asp Phe Ser Ser Glu Gly Ala Thr Ala Ile Leu Pro85 90 95Pro Glu Met Asp Glu Phe Tyr Gly Ser Met Ile Asn Met Asp Asn Pro100 105 110Glu His Ser Arg Leu Arg Arg Ile Val Ala Arg Ser Phe Gly Arg Gly115 120 125Met Ala Pro Lys Phe Asp Ala Met Ser Arg Arg Val Ala Arg Arg Ile130 135 140Val Asp Glu Leu Ile Glu Arg Gly Pro Gly Asp Phe Ile Arg Pro Ala145 150 155 160Ala Glu Met Pro Ile Ala Val Leu Ser Thr Met Met Gly Ile Pro Gly165 170 175Glu Asp Tyr Glu Phe Leu Phe Glu Arg Thr Asn Thr Ile Met Gly Gly180 185 190Ala Asp Pro Glu Leu Ala Ala Asp Pro Glu Lys Met Ala Ala Ala Val195 200 205Leu Gly Ala Leu Arg Asp Leu Gly Asp Tyr Ile Gly Arg Leu Arg Glu210 215 220Asp Arg Leu Ala Arg Pro Gly Pro Asp Val Ile Thr Lys Leu Val Gln225 230 235 240Val Gln Glu Asp Gly Glu Gln Leu Thr Asn Gln Glu Leu Val Ser Phe245 250 255Phe Ile Leu Leu Ile Asn Ala Gly Met Glu Thr Thr Arg Asn Val Ile260 265 270Ala Gln Ala Leu Val Leu Leu Thr Glu His Pro Asp Gln Arg Gln Leu275 280 285Leu Leu Ser Asp Phe Glu Leu His Ala Lys Gly Ala Val Glu Glu Ile290 295 300Leu Arg Val Gly Thr Pro Ile Asn Trp Met Arg Arg Thr Ala Thr Gly305 310 315 320Asp Cys Glu Met Asn Gly His Arg Phe Arg Lys Gly Asp Glu Ile Phe325 330 335Leu Phe Tyr Trp Ser Ala Asn His Asp Glu Lys Val Phe Glu Asp Ala340 345 350Tyr Arg Phe Asp Ile Thr Arg Asp Pro Asn Pro His Leu Ser Phe Gly355 360 365Ala Val Gly Pro His Phe Cys Leu Gly Ala His Leu Ala Arg Ile Glu370 375 380Ile Ile Ala Met Leu Arg Glu Leu Leu Ala Ser Leu Pro Asp Ile Arg385 390 395 400Val Glu Gly Glu Pro Val Arg Leu Ala Ser Ser Phe Ile Glu Gly Phe405 410 415Lys Glu Leu Ser Cys Thr Phe420<210> 30<211> 335<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Val Thr Ile Arg Asp Val Ala Lys Ala Ser Gly Val Ser Pro Ser Thr1 5 10 15Val Ser Arg Ala Leu Ala Pro Gly Gly Ala Val Ser Pro Val Thr Arg20 25 30Glu Arg Val Arg Ala Ala Ala Asp Arg Leu Gly Tyr Gln Pro Asn Gln35 40 45Ala Ala Arg Gly Leu Ile Thr Gly Arg Thr Gly His Leu Gly Val Ile50 55 60Val Pro Asp Leu Leu Asn Pro Phe Phe Ala Asp Ile Cys Lys Gly Val65 70 75 80Gln Ala Arg Ala Arg Gly Leu Gly Leu Thr Val Phe Val Ser Asp Thr85 90 95Glu Arg Asp Glu Gly Leu Glu Leu Asp Ala Ile Arg Thr Leu Ala Pro
100 105 110Gln Val Asp Gly Ile Val Leu Cys Ser Pro His Leu Ser Gly Glu Glu115 120 125Leu Gly Ser Leu Gly Asp Phe Thr Asp Lys Pro Ile Val Leu Leu His130 135 140Arg Lys Glu Pro Gly Phe Gly Ser Val Thr Ala Asp Leu Val Glu Gly145 150 155 160Met Thr Asp Ala Leu Thr His Leu His Ala Leu Gly His Arg Arg Ile165 170 175Ala Tyr Val Gly Gly Pro Arg Ser Ser Trp Ala Ala Arg Glu Arg Ala180 185 190Ala Gly Val Glu Ala Val Ala Ala Ser Gly Leu Val Glu Ile Val Gln195 200 205Val Gly Ser Val Ala Pro His Phe Asp Gly Gly Val Thr Gly Ala Ala210 215 220Asp Val Val Leu Ala Ser Gly Ala Ser Ala Val Leu Ala Phe Asp Asp225 230 235 240Ile Val Ala Phe Gly Leu Ile Ser Arg Phe Thr Val Arg Gly Val Arg245 250 255Val Pro Glu Glu Met Ser Val Val Gly Cys Asp Asp Ile Ala Leu Ser260 265 270Gly Met Ala Ala Pro Pro Leu Thr Thr Val Ser Val Pro Lys Ala His275 280 285Gly Ala Arg Ala Ala Val Asp Leu Leu Cys Arg Ile Leu Ala Thr Pro290 295 300Ala Ala Glu Gly Glu Gln Pro Pro Gln Arg Val Leu Pro Thr His Leu305 310 315 320Val Val Arg Gly Ser Thr Ala Ala Leu Asp Arg Arg Gln Arg Ala325 330 335<210> 31<211> 313<212> PRT<213> 南昌链霉菌NS3226(Streptomyces nanchangensis n.sp.NS3226)<400> 1Met Asp Pro Leu Ala Gly Leu Leu Asp Gly Pro Arg Ala Arg Gly Ala1 5 10 15Phe Leu Leu Arg Met Val Met Asp Pro Pro Trp Ser Val Arg Ile Glu20 25 30Asp Arg Ala Pro Leu Cys Val Met Thr Val Ala Arg Gly Glu Ala Trp35 40 45Val Val Pro Asp Arg Gly Glu Ala Arg Arg Leu Gly Pro Gly Asp Val50 55 60Ala Val Val Arg Gly Pro Asp Pro Tyr Thr Val Ala Gly Asp Pro Ala65 70 75 80Thr Glu Pro Gln Ala Trp Ile Leu Pro Gly Glu Val Cys Arg Thr Ala85 90 95Gly Gly Glu Asp Leu Ala Glu Arg Met Ala Leu Gly Val Arg Thr Trp100 105 110Gly Asn Ser Glu His Gly Ala Thr Thr Met Leu Val Gly Thr Tyr Arg115 120 125Met Asp Gly Glu Ile Ser Arg Arg Leu Leu Asp Ala Leu Pro Pro Leu130 135 140Leu Val Leu Gly Arg Glu Arg Cys Asp Ser Pro Leu Leu Pro Trp Leu145 150 155 160Gly Glu Glu Ile Val Lys Glu Glu Ala Gly Gln Thr Ala Val Leu Asp165 170 175Arg Leu Leu Asp Leu Leu Leu Ile Ser Val Leu Arg Ala Trp Phe Ala180 185 190Arg Pro Glu Ala Arg Ala Pro Ala Trp Tyr Arg Ala Leu Gly Asp Pro195 200 205Val Val Gly Arg Ala Leu Arg Leu Leu Gln Asn Asn Pro Gly His Pro210 215 220Trp Thr Val Ala Leu Leu Ala Ala Glu Thr Gly Ile Ser Arg Ala Val225 230 235 240Leu Ala Arg Arg Phe Thr Glu Leu Val Gly Glu Pro Pro Met Ala Tyr245 250 255Leu Thr Gly Trp Arg Leu Asp Leu Ala Ala Asp Leu Leu Arg Glu Pro260 265 270Asp Ala Thr Leu Gly Ala Val Ala Arg Arg Val Gly Tyr Gly Ser Ser275 280 285Phe Ala Leu Ser Ala Ala Phe Lys Arg Val Arg Gly Val Ser Pro Arg290 295 300Glu His Arg Ser Ala Ala Ser Ala Gly305 310
权利要求
1.一种南昌霉素生物合成基因簇，其特征在于整个南昌霉素生物合成基因簇共30个基因，具体为(1)聚酮合酶基因，即nanA1，nanA2，nanA3，nanA4，nanA5，nanA6，nanA7，nanA8，nanA9，nanA10，nanA11共11个基因；(2)南昌霉素的修饰基因，即nanE，nanI，nanO，nanP共4个基因；(3)南昌霉素脱氧糖的生物合成基因，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM共6个基因；(4)南昌霉素的调节基因，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5共9个基因。
2.根据权利要求1所述的南昌霉素生物合成基因簇，其特征是所述的聚酮合酶基因，其编码催化南昌链霉菌NS3226中聚醚类离子载体抗生素南昌霉素聚酮糖苷配基生物合成所需的11个I型聚酮合酶开放读码框，即nanA1，nanA2，nanA3，nanA4，nanA5，nanA6，nanA7，nanA8，nanA9，nanA10，nanA11的核苷酸序列或互补序列及其相应的氨基酸序列。
3.根据权利要求2所述的南昌霉素生物合成基因簇，其特征是11个开放读码框所包含的I型聚酮合酶模块或结构域，即酮基合成酶结构域(KS)、酰基转移酶结构域(AT)、酮基还原酶结构域(KR)、脱水酶结构域(DH)、烯酰基还原酶结构域(ER)、酰基载体蛋白结构域(ACP)、链释放结构域(CR)的核苷酸序列或互补序列及其相应的氨基酸序列。
4.根据权利要求1所述的南昌霉素生物合成基因簇，其特征是所述的南昌霉素的修饰基因，其编码参与南昌霉素聚酮链修饰的4个开放读码框，即nanE，nanI，nanO，nanP的核苷酸序列或互补序列及其相应的氨基酸序列。
5.根据权利要求1所述的南昌霉素生物合成基因簇，其特征是所述的南昌霉素脱氧糖的生物合成基因，其编码参与南昌霉素生物合成中脱氧糖(4-O-甲基-L-rhodinose)的合成及转移的6个开放读码框，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM的核苷酸序列或互补序列及其相应的氨基酸序列。
6.根据权利要求1所述的南昌霉素生物合成基因簇，其特征是所述的南昌霉素的调节基因，其编码参与南昌霉素生物合成调节的9个开放读码框，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5的核苷酸序列或互补序列及其相应的氨基酸序列。
全文摘要
一种作为聚醚类抗生素的南昌霉素生物合成基因簇属于基因技术领域。整个南昌霉素生物合成基因簇共30个基因，具体为(1)聚酮合酶基因，即nanA1，nanA2，nanA3，nanA4，nanA5，nanA6，nanA7，nanA8，nanA9，nanA10，nanA11共11个基因；(2)南昌霉素的修饰基因，即nanE，nanI，nanO，nanP共4个基因；(3)南昌霉素脱氧糖的生物合成基因，即nanG1，nanG2，nanG3，nanG4，nanG5，nanM共6个基因；(4)南昌霉素的调节基因，即nanR1，nanR2，nanR3，nanR4，nanT1，nanT2，nanT3，nanT4，nanT5共9个基因。本发明所提供的基因及其蛋白质，抗体也可以用来查找和发展可用于医药，工业，农业的化合物或蛋白。
文档编号C12N15/11GK1431220SQ03114920
公开日2003年7月23日 申请日期2003年1月16日 优先权日2003年1月16日
发明者邓子新, 孙宇晖, 周秀芬, 涂国全 申请人:上海交通大学, 江西农业大学