甜菊醇糖苷组合物,生产方法和用途与流程

文档序号:17432410发布日期:2019-04-17 03:40阅读:198来源:国知局
发明领域本发明涉及用于从甜叶菊(steviarebaudiana)植物生产纯化的食品成分的方法,及其在各种食品和饮料中的用途。相关领域的描述由于意识到许多疾病与高糖食品和饮料的食用有关,如今越来越关注糖替代品。然而,许多人造甜味剂,如甜精(dulcin)、甜蜜素(cyclamate)钠和糖精,由于对它们的安全性的关注,在一些国家被禁止或限制。因此,天然来源的无热量甜味剂变得越来越普及。甜味草本植物甜叶菊(steviarebaudianabertoni)产生多种二萜糖苷,其特征在于优于许多其他高效力甜味剂的高强度甜味和感官特性。上述甜味糖苷具有共同的糖苷配基甜菊醇(steviol),而区别在于c13和c19位置的碳水化合物残基的数量和类型。甜叶菊的叶子能够累积高达10-20%(基于干重)甜菊醇糖苷。甜叶菊叶子中发现的主要糖苷是莱鲍迪苷a(2-10%)、甜菊苷(2-10%)和莱鲍迪苷c(1-2%)。其他甜菊醇糖苷,如莱鲍迪苷b、d、e、f、g、h、i、j、k、l、m、n和o,甜菊双糖苷、悬钩子苷等,也以低水平被发现。甜菊醇糖苷不仅彼此的分子结构不同,其味道特性也不同。通常,发现甜菊苷比蔗糖甜110-270倍,莱鲍迪苷a比蔗糖甜150至320倍,而莱鲍迪苷c比蔗糖甜40-60倍。杜尔可苷a比蔗糖甜30倍。已知许多甜菊醇糖苷在纯化形式下具有低溶解度。使用水或有机溶剂从甜叶菊植物提取和纯化甜味糖苷的方法描述于例如美国专利号4,361,697;4,082,858;4,892,938;5,972,120;5,962,678;7,838,044和7,862,845中。本发明提供了现有技术中未描述过的、可替换的、更有效的用于甜菊醇糖苷纯化的方法。发明概述在一个实施方案中,本发明涉及用于生产甜菊醇糖苷组合物的方法,其包括:提供包含甜叶菊(steviarebaudiana)植物的至少一种甜菊醇糖苷的原料;将原料溶解于含水醇中,以获得原料溶液;在约1分钟至约240小时的时间间隔期间将原料溶液保持在约-24℃至约80℃的温度范围下,以获得结晶的甜菊醇糖苷组合物;将结晶的甜菊醇糖苷组合物与母液分离,以获得分离的甜菊醇糖苷组合物;并将分离的甜菊醇糖苷组合物干燥,以产生甜菊醇糖苷组合物。在一个实施方案中,通过这种方法制得的甜菊醇糖苷组合物在20℃的水中具有至少0.01%的溶解度。通过以上方法制得的甜菊醇糖苷组合物可以用作甜味剂组合物或风味组合物,或包括在甜味剂组合物或风味组合物中。所述甜菊醇糖苷组合物还可以用作食品成分,或可以包括在食品、饮料、化妆品或药品中。发明详述本发明的目的在于克服现有的甜菊醇糖苷纯化方法的缺陷。本发明部分涉及用于生产甜菊醇糖苷组合物的方法,所述组合物包含甜叶菊植物的至少一种甜菊醇糖苷,其选自甜菊苷、莱鲍迪苷a、莱鲍迪苷b、莱鲍迪苷c、莱鲍迪苷d、莱鲍迪苷e、莱鲍迪苷f、莱鲍迪苷g、莱鲍迪苷h、莱鲍迪苷i、莱鲍迪苷j、莱鲍迪苷k、莱鲍迪苷l、莱鲍迪苷m、莱鲍迪苷n、莱鲍迪苷o、杜尔可苷a、甜菊双糖苷和悬钩子苷,以及甜叶菊植物中发现的其他甜菊醇糖苷及其混合物。在一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷a含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷b含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷c含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷d含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷e含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷f含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷g含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷h含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷i含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷j含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷k含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷l含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷m含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷n含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的莱鲍迪苷o含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的甜菊苷含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的杜尔可苷a含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的悬钩子苷含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的甜菊双糖苷含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。在另一个实施方案中,根据所要求的发明生产的甜菊醇糖苷组合物中的甜叶菊中发现的任何甜菊醇糖苷的含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或100%。所述方法中使用的原料包含甜叶菊植物的至少一种甜菊醇糖苷,其选自甜菊苷、莱鲍迪苷a、莱鲍迪苷b、莱鲍迪苷c、莱鲍迪苷d、莱鲍迪苷e、莱鲍迪苷f、莱鲍迪苷g、莱鲍迪苷h、莱鲍迪苷i、莱鲍迪苷j、莱鲍迪苷k、莱鲍迪苷l、莱鲍迪苷m、莱鲍迪苷n、莱鲍迪苷o、杜尔可苷a、甜菊双糖苷和悬钩子苷,以及甜菊植物中发现的其他甜菊醇糖苷及其混合物。在一个实施方案中,原料中的所述莱鲍迪苷a含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷b含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷c含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷d含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷e含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷f含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷g含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷h含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷i含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷j含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷k含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷l含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷m含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷n含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述莱鲍迪苷o含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述甜菊苷含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述杜尔可苷a含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述悬钩子苷含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述甜菊双糖苷含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。在另一个实施方案中,原料中的所述甜叶菊中发现的任何甜菊醇糖苷的含量(%w/w,干基)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%或99%。将原料与含水醇混合,并任选加热至约25℃至约100℃的温度范围,以获得原料溶液。在一个实施方案中,含水醇包含(%v/v)约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96%,97%,98%,99%或99.9%乙基醇(乙醇)。所获得的溶液中的原料浓度(%w/v)为约0.05%,0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1%,1.5%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%或70%。在一个实施方案中,将原料溶液在约-24℃至约80℃的温度范围下保持约1分钟至约240小时的时间间隔范围。在一个实施方案中,将原料溶液任选用甜菊醇糖苷种子组合物接种,以促进甜菊醇糖苷组合物的结晶。所述甜菊醇糖苷种子组合物包含甜叶菊植物的至少一种甜菊醇糖苷,其选自甜菊苷、莱鲍迪苷a、莱鲍迪苷b、莱鲍迪苷c、莱鲍迪苷d、莱鲍迪苷e、莱鲍迪苷f、莱鲍迪苷g、莱鲍迪苷h、莱鲍迪苷i、莱鲍迪苷j、莱鲍迪苷k、莱鲍迪苷l、莱鲍迪苷m、莱鲍迪苷n、莱鲍迪苷o、杜尔可苷a、甜菊双糖苷和悬钩子苷,以及甜叶菊植物中发现的其他甜菊醇糖苷及其混合物。在一个实施方案中,将原料溶液在约-24℃,-23℃,-22℃,-21℃,-20℃,-19℃,-18℃,-17℃,-16℃,-15℃,-14℃,-13℃,-12℃,-11℃,-10℃,-9℃,-8℃,-7℃,-6℃,-5℃,-4℃,-3℃,-2℃,-1℃,0℃,1℃,2℃,3℃,4℃,5℃,6℃,7℃,8℃,9℃,10℃,11℃,12℃,13℃,14℃,15℃,16℃,17℃,18℃,19℃,20℃,21℃,22℃,23℃,24℃,25℃,26℃,27℃,28℃,29℃,30℃,31℃,32℃,33℃,34℃,35℃,36℃,37℃,38℃,39℃,40℃,41℃,42℃,43℃,44℃,45℃,46℃,47℃,48℃,49℃,50℃,51℃,52℃,53℃,54℃,55℃,56℃,57℃,58℃,59℃,60℃,61℃,62℃,63℃,64℃,65℃,67℃,68℃,69℃,70℃,71℃,72℃,73℃,74℃,75℃,76℃,77℃,78℃,79℃或80℃下保持约1分钟至约240小时的时间间隔范围。在一个实施方案中,将原料溶液在约-24℃至约80℃的温度范围下保持约1分钟,2分钟,3分钟,4分钟,5分钟,6分钟,7分钟,8分钟,9分钟,10分钟,11分钟,12分钟,13分钟,14分钟,15分钟,16分钟,17分钟,18分钟,19分钟,20分钟,21分钟,22分钟,23分钟,24分钟,25分钟,26分钟,27分钟,28分钟,29分钟,30分钟,31分钟,32分钟,33分钟,34分钟,35分钟,36分钟,37分钟,38分钟,39分钟,40分钟,41分钟,42分钟,43分钟,44分钟,45分钟,46分钟,47分钟,48分钟,49分钟,50分钟,51分钟,52分钟,53分钟,54分钟,55分钟,56分钟,57分钟,58分钟,59分钟或60分钟的时间间隔。在一个实施方案中,将原料溶液在约-24℃至约80℃的温度范围下保持约1小时,2小时,3小时,4小时,5小时,6小时,7小时,8小时,9小时,10小时,11小时,12小时,13小时,14小时,15小时,16小时,17小时,18小时,19小时,20小时,21小时,22小时,23小时,24小时,25小时,26小时,27小时,28小时,29小时,30小时,31小时,32小时,33小时,34小时,35小时,36小时,37小时,38小时,39小时,40小时,41小时,42小时,43小时,44小时,45小时,46小时,47小时,48小时,49小时,50小时,51小时,52小时,53小时,54小时,55小时,56小时,57小时,58小时,59小时,60小时,61小时,62小时,63小时,64小时,65小时,66小时,67小时,68小时,69小时,70小时,71小时,72小时,73小时,74小时,75小时,76小时,77小时,78小时,79小时,80小时,81小时,82小时,83小时,84小时,85小时,86小时,87小时,88小时,89小时,90小时,91小时,92小时,93小时,94小时,95小时,96小时,97小时,98小时,99小时,100小时,101小时,102小时,103小时,104小时,105小时,106小时,107小时,108小时,109小时,110小时,111小时,112小时,113小时,114小时,115小时,116小时,117小时,118小时,119小时,120小时,121小时,122小时,123小时,124小时,125小时,126小时,127小时,128小时,129小时,130小时,131小时,132小时,133小时,134小时,135小时,136小时,137小时,138小时,139小时,140小时,141小时,142小时,143小时,144小时,145小时,146小时,147小时,148小时,149小时,150小时,151小时,152小时,153小时,154小时,155小时,156小时,157小时,158小时,159小时,160小时,161小时,162小时,163小时,164小时,165小时,166小时,167小时,168小时,169小时,170小时,171小时,172小时,173小时,174小时,175小时,176小时,177小时,178小时,179小时,180小时,181小时,182小时,183小时,184小时,185小时,186小时,187小时,188小时,189小时,190小时,191小时,192小时,193小时,194小时,195小时,196小时,197小时,198小时,199小时,200小时,201小时,202小时,203小时,204小时,205小时,206小时,207小时,208小时,209小时,210小时,211小时,212小时,213小时,214小时,215小时,216小时,217小时,218小时,219小时,220小时,221小时,222小时,223小时,224小时,225小时,226小时,227小时,228小时,229小时,230小时,231小时,232小时,233小时,234小时,235小时,236小时,237小时,238小时,239小时或240小时。在一个实施方案中,将从原料溶液结晶的所述甜菊醇糖苷组合物与母液(液相)分离,并干燥,产生含有低于6%水分的粉末状甜菊醇糖苷组合物。通过在2小时过程中在105℃下的“干燥失重”方法(jecfavol.4)来测定水分。通过将粉末状甜菊醇糖苷组合物溶解于20℃去离子水中来测定所获得的甜菊醇糖苷组合物水溶性(%w/v)。如果在10分钟的搅动内完全溶解并且产生可稳定24小时的澄清溶液(<10fau),则认为所述材料是可溶的。在一个实施方案中,所述粉末状的甜叶菊醇糖苷组合物的水溶性(%w/v)不低于约0.01%,0.02%,0.03%,0.04%,0.05%,0.06%,0.07%,0.08%,0.09%,0.10%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%,1.0%,1.1%,1.2%,1.3%,1.4%,1.5%,1.6%,1.7%,1.8%,1.9%,2.0%,2.1%,2.2%,2.3%,2.4%,2.5%,2.6%,2.7%,2.8%,2.9%,3.0%,3.1%,3.2%,3.3%,3.4%,3.5%,3.6%,3.7%,3.8%,3.9%,4.0%,4.1%,4.2%,4.3%,4.4%,4.5%,4.6%,4.7%,4.8%,4.9%,5.0%,5.5%,6.0%,6.5%,7.0%,7.5%,8.0%,8.5%,9.0%,9.5%或10.0%。本发明的甜菊醇糖苷组合物可以用作各种食品和饮品中的甜味剂、甜味增强剂、风味剂和风味增强剂。食品和饮品的非限制性实例包括碳酸软饮料,包括但不限于可乐风味的碳酸软饮料、水果风味的碳酸软饮料、浆果风味的碳酸软饮料、即饮饮料、能量饮料、等渗饮料、低卡路里饮料、零卡路里饮料、运动饮料、茶、水果和蔬菜汁、汁液饮料、乳饮料、酸奶饮料、醇饮料、粉末饮料、烘焙制品、小甜饼、饼干、烘焙混合物、谷物、糖食、糖果、太妃糖、口香糖、乳制品、风味乳、酸奶、调味酸奶、发酵奶、酱油和其他大豆基产品、沙拉酱、蛋黄酱、醋、冷冻甜点、肉制品、鱼肉制品、瓶装和罐装食品、桌面甜味剂,以及水果和蔬菜。另外,本发明的甜菊醇组合物可以用于药物或药物制剂和化妆品,包括但不限于,牙膏、漱口水、咳嗽糖浆、咀嚼片、锭剂、维生素制剂等中。本发明的甜菊醇糖苷组合物可以“按原样”使用或与其他甜味剂、风味剂和食品成分结合使用。甜味剂的非限制性实例包括莱鲍迪苷a、莱鲍迪苷b、莱鲍迪苷c、莱鲍迪苷d、莱鲍迪苷e、莱鲍迪苷f、莱鲍迪苷g、莱鲍迪苷h、莱鲍迪苷i、莱鲍迪苷j、莱鲍迪苷k、莱鲍迪苷l、莱鲍迪苷m、莱鲍迪苷n、莱鲍迪苷o、杜尔可苷a、甜菊双糖苷、悬钩子苷,以及甜叶菊植物中发现的其他甜菊醇糖苷及其混合物,甜菊提取物,糖基化的甜菊醇糖苷,通过化学、酶合成或通过重组微生物发酵而制得的甜菊醇糖苷,罗汉果提取物,罗汉果苷,糖基化的罗汉果苷,高果糖玉米糖浆,玉米糖浆,转化糖,低聚果糖,菊粉,菊粉寡糖,偶联糖,低聚麦芽糖,麦芽糖糊精,糊精,极限糊精,玉米糖浆干粉,葡萄糖,麦芽糖,蔗糖,乳糖,阿洛酮糖,塔格糖,阿斯巴甜,糖精,三氯蔗糖,糖醇及其混合物。风味剂的非限制性实例包括可乐、柠檬、酸橙、橙子、葡萄柚、香蕉、葡萄、苹果、梨、菠萝、苦杏仁、肉桂、糖、棉花糖、香草香精,糖基化的甜菊醇糖苷,nsf02及其混合物。其他食品成分的非限制性实例包括风味剂、酸化剂、有机酸和氨基酸、着色剂、填充剂、改性淀粉、树胶、组织形成剂、防腐剂、抗氧化剂、维生素、乳化剂、稳定剂、增稠剂、胶凝剂、咖啡因、茶氨酸、可可碱及其混合物。以下实施例说明了本发明的一些实施方案。应当明白本发明不限于实施例中列出的材料、比例、条件和程序,其只是说明性的。实施例1甜菊醇糖苷组合物的制备将14kg由purecirclesdnbhd(马来西亚)制造的起始甜叶菊提取物(sse;批次#c4-0001-1015-0011)加入112升75%(v/v)的乙醇中。将混合物加热至80℃,用于sse的完全溶解。随后将溶液温度降至25℃,并且加入约3g晶体莱鲍迪苷m作为种子。将溶液在25℃下保持96小时,用于结晶。将获得的晶体与母液分离,并且在篮式离心机中用纯乙醇洗涤。将湿晶体在真空干燥机(70℃)中干燥。获得了约4kg粉末状的甜菊醇糖苷组合物(sgc-1)。所获得的组合物在20℃去离子水中的溶解度为0.05%(w/v)。通过以下的hplc方法分析了sse和甜菊醇糖苷组合物(sgc-1)。hplc系统:配备有双泵的agilent1200系列系统,自动取样机,柱温箱和dad检测仪。柱:poroshell120sb-c18,4.6×150mm,2.7μm,40℃移动相:预混物1:乙腈中75%(v/v)10mmol/l(ph2.6)磷酸盐缓冲液预混物2:乙腈中68%(v/v)10mmol/l(ph2.6)磷酸盐缓冲液梯度程序:时间(分钟)预混物1(%)预混物2(%)0.0100012.0100012.5505013.0010040.00100流速:1.0ml/分钟注射体积:5μl检测仪:uv210nm运行时间:40分钟样品:稀释液(乙腈中70%(v/v)水)中大约1,200mg/lhplc分析结果提供于表1中。表1sse和sgc-1的hplc分析结果实施例2甜菊醇糖苷组合物的制备将14kg由purecirclesdnbhd(马来西亚)制造的起始甜菊提取物(sse;批次#c4-0001-1015-0011)加入112升75%(v/v)的乙醇中。将混合物加热至80℃,用于sse的完全溶解。随后将溶液温度降至25℃,并且在25℃下保持144小时,用于结晶。没有加入晶体莱鲍迪苷m种子,因此与实施例1相比,结晶较慢。将获得的晶体与母液分离,并且在篮式离心机中用纯乙醇洗涤。将湿晶体在真空干燥机(70℃)中干燥。获得了约3.8kg粉末状的甜菊醇糖苷组合物(sgc-2),具有1.5%(w/w)含水量和0.16%(w/w)残余乙醇含量。sgc-2样品在20℃去离子水中的溶解度为0.5%(w/v),即比实施例1的sgc-1的溶解度高约10倍。使用实施例1中所述的hplc方法分析了sse和sgc-2。hplc分析结果提供于表2中。表2sse和sgc-2的hplc分析结果将起始甜叶菊提取物(sse)和获得的甜菊醇糖苷组合物(sgc-2)用于以下的味道评价实验中。实施例3低卡路里橙汁饮料将橙子浓缩物(35%)、柠檬酸(0.35%)、抗坏血酸(0.05%)、橙红色素(0.01%)、橙子香精(0.20%)和0.05%起始甜叶菊提取物(sse)或实施例2的甜菊醇糖苷组合物(sgc-2)混合并完全溶于水中(高达100%),并且巴氏杀菌。样品的感官评价概括于表3中。数据显示出通过使用根据实施例2获得的甜菊醇糖苷组合物可以获得最佳结果。特别地,用所述组合物制备的饮料呈现出全面完整的风味特征和口感。表3橙汁饮料样品的评价可以使用相同的方法来制备其他水果,如苹果、柠檬、杏、浆果、菠萝、芒果等的汁液和汁液饮料。实施例4零卡路里碳酸饮料根据表4中呈现的配方制备了碳酸饮料样品。表4碳酸饮料配方通过20名专家评价感官特性。结果概括于表5中。表5零卡路里碳酸饮料样品的评价以上结果表明使用根据实施例2获得的甜菊醇糖苷组合物制备的饮料具有更好的感官特征。实施例5减肥饼干将面粉(50.0%)、人造黄油(30.0%)、果糖(10.0%)、麦芽糖醇(8.0%)、全脂奶(1.0%)、盐(0.2%)、烘焙粉(0.15%)、香草(0.1%)和起始甜菊提取物(sse)或实施例2的甜菊醇糖苷组合物(sgc-2)(0.03%)在面团混合机中充分捏合。将获得的面团用模具造形,并且在200℃的烤箱中烘烤15分钟。通过20名专家评价了感官特性。在含有根据实施例2获得的甜菊醇糖苷组合物的样品中获得了最佳结果。专家注意到更全面完整的风味特征和口感。实施例6酸奶将起始甜菊提取物(sse)或实施例2的甜菊醇糖苷组合物(sgc-2)(0.03%)和蔗糖(4%)溶解于低脂奶中。在82℃下巴氏杀菌20分钟后,将奶冷却至37℃。加入起始培养物(3%),并且将混合物在37℃下孵育6小时,随后在5℃下孵育12小时。由20名专家评价了感官特性。在含有根据实施例2获得的甜菊醇糖苷组合物的样品中获得了最佳结果。专家注意到更全面的风味特征和口感。尽管已经详细描述了本发明及其优点,但应当明白可以在本文中进行各种变化、替换和改变,而不脱离由所附权利要求限定的本发明的精神和范围。此外,没有打算将本申请的范围限于说明书中描述的本发明的特定实施方案。因为本领域普通技术人员从本发明的公开内容将易于认识到,根据本发明可以利用目前现有的或之后待研发的与本文中所述的相应实施方案执行基本上相同功能或获得基本上相同结果的组合物、过程、方法和步骤。当前第1页12
当前第1页1 2 
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