CRISPR-CAS9抑制剂的制作方法

本申请要求2016年11月16日提交的美国临时专利申请号62/422,850的优先权，其通过引用纳入本文用于所有目的。

背景技术：

防止病毒攻击的能力是细胞生命的标志。细菌采用多种机制抵抗细菌病毒(噬菌体)的感染，包括限制酶和crispr-cas系统(labrie,s.j.,samson,j.e.和moineau,s.(2010).natrevmicro,8,317–327)。由于其规律成簇的间隔短回文重复之间有小的间隔子序列，crispr阵列具有先前与可动遗传因子相遇的序列特异性残余(mojica,f.j.m等.(2005).j.mol.evol.,60,174–182)。采用这些间隔子来产生向导rna，它们促进经编程靶标的结合与切割(brouns,s.j.j等.(2008).science,321,960–964；garneau,j.e.等.(2010).nature,468,67–71)。免疫功能所需的crispr相关的(cas)基因常存在于crispr阵列附近(marraffini,l.a.(2015).原核生物中的crispr-cas免疫(crispr-casimmunityinprokaryotes).nature,526,55–61；wright,a.v.,j.k.和doudna,j.a.(2016).cell,164,29–44)。cas蛋白不仅进行外来基因组的破坏(garneau,j.e.等.(2010).nature,468,67–71)，还促进成熟crisprrna(crrnas)的生成(deltcheva；haurwitz,r.e等.(2010).science,329,1355–1358)以及将外来序列收进crispr阵列(j.k.等.(2014).nat.struct.mol.biol,21,528-534；yosef,i.,goren,m.g.和qimron,u.(2012).nucleicacidsresearch,40,5569–5576)。

crispr-cas适应性免疫系统在细菌世界中是常见且多样的。细菌基因组中已鉴定出六种不同类型(i-vi)(abudayyeh,o.o等.(2016).scienceaaf5573；makarova,k.s.等.(2015).natrevmicro,13,722–736).natrevmicro,13,722–736)，其有能力按rna向导的指定切割靶dna或rna序列。crispr-cas系统的简易可编程性已得到广泛开发，为许多新型遗传技术打开了大门(barrangou,r.和doudna,j.a.(2016)，naturebiotechnology,34,933–941)。这些技术中的大多数使用来自酿脓链球菌(streptococcuspyogenes，spy)的cas9，以及经工程改造的单向导rna作为此类应用的基础，包括动物细胞中的基因编辑(cong,l.等.(2013).science339,819–823；jinek,m.等(2012).science,337,816–821；mali,p.等(2013).science,339,823–826；qi,l.s.等(2013).cell,152,1173–1183)。此外，已经研究了ii-a亚型内的cas9直系同源物用于基因编辑应用(ran,f.a.等(2015).nature520,186–191)，并且新2类crispr单蛋白效应物如cpf1(v型(zetsche,b.等(2015).cell,163,759–771))和c2c2(vi型(abudayyeh,o.o等(2016).scienceaaf5573；east-seletsky,a.等(2016).nature538,270-273)正在表征中。1类crispr-cas系统(i、iii和iv型)是rna引导的多蛋白复合物，因此它们因其复杂性而被大多数基因组应用忽视。然而，这些系统在近一半的细菌和约85％的古细菌中是最常见的(makarova,k.s.等(2015).natrevmicro,13,722–736).natrevmicro,13,722–736)。

为了应对细菌对噬菌体感染的战争，噬菌体反过来经常编码细菌免疫系统的抑制剂，这增强了它们裂解宿主细菌或整合到其基因组中的能力(samson,j.e.等(2013).natrevmicro,11,675–687)。噬菌体编码的“抗crispr”蛋白的第一个例子来自铜绿假单胞菌(pseudomonasaeruginosa)的(1类)i-e和i-f型系统(bondy-denomy等(2013).nature,493,429–432；pawluk,a.等(2014).mbio5,e00896)。值得注意的是，到目前为止已经发现了十种i-f型抗crispr和四种i-e型抗crispr基因(pawluk,a.等(2016).naturemicrobiology,1,1–6)，所有这些都编码不同的小蛋白质(50-150个氨基酸)，先前功能未知。我们对4种i-f抗crispr蛋白的生化研究表明，它们与多蛋白crispr-cas复合物中的不同cas蛋白直接相互作用，以防止靶dna的识别或切割(bondy-denomy,j等(2015).nature,526,136–139)。各蛋白质具有不同序列、结构和作用模式(maxwell,k.l.等(2016).naturecommunications,7,13134；wang,x.(2016).nat.struct.mol.biol23,868-870)。这些发现支持crispr-cas抑制剂的独立进化，并表明还有更多尚未发现。鉴于此，近期的一篇论文利用了具有预测的螺旋-转角-螺旋(hth)基序的特征性抗crispr相关(aca)基因的保守性来鉴定铜绿假单胞菌(p.aeruginosa)外的抗crispr基因。这导致作者在变形菌门中发现抗crispr，其广泛地跨越i-f型crispr-cas系统发育(pawluk,a.等(2016).naturemicrobiology,1,1–6)。这表明所有crispr系统都可能存在抗crispr，并且需要能够发现它们的方法。

铜绿假单胞菌中的i型抗crispr从整合的噬菌体基因组(原噬菌体)表达，导致宿主crispr-cas系统的组成型失活。(bondy-denomy等(2013).nature,493,429–432。这通常会导致原噬菌体具有与同一细胞中共同出现的crispr间隔子完全一致的dna靶标的情况，称为“自靶向”(图1a)。这种情况使得crispr-cas灭活成为生存的必要条件，因为在没有原噬菌体抗crispr基因的情况下，宿主基因组在靶向原噬菌体的过程中被切割(bondy-denomy等(2013).nature,493,429–432；edgar,r.和qimron,u.(2010).j.bacteriol192,6291–6294)。抗crispr的表达会中和该风险，然而，这将允许溶素原存活。我们推测，具有明显自靶向的crispr系统的基因组将是鉴定新crispr-cas抑制剂的候选者。我们在此描述了使用生物信息学方法在单核细胞增生李斯特菌(listeriamonocytogenes)中鉴定以前未知的噬菌体编码的crispr-cas9抑制剂，以识别自靶向事件。我们显示这些抑制剂中的两种也可以阻断酿脓链球菌cas9在细菌细胞和人细胞中的活性。

定义

术语“核酸”或“多核苷酸”是指单链或双链形式的脱氧核糖核酸(dna)或核糖核酸(rna)及其聚合物。除非特别限定，否则该术语涵盖含有天然核苷酸的已知类似物的核酸，其具有与参比核酸相似的结合特性，并以与天然存在的核苷酸相似的方式代谢。除非另有说明，否则具体核酸序列还隐含地包括其保守修饰的变体(例如，简并密码子取代)，等位基因，直系同源物，snp和互补序列以及明确指出的序列。具体地，简并密码子取代可以通过产生其中一个或多个选定(或所有)密码子的第三位被混合碱基和/或脱氧肌苷残基取代的序列来实现(batzer等,nucleicacidres.19:5081(1991)；ohtsuka等,j.biol.chem.260:2605-2608(1985)；和rossolini等,mol.cell.probes8:91-98(1994))。

术语“基因”是指参与产生多肽链的dna区段。它可以包括编码区之前和之后的区域(前导区和尾区)以及各个编码区段(外显子)之间的间插序列(内含子)。

“启动子”定义为指导核酸转录的核酸控制序列的阵列。如本文所用，启动子包括转录起始位点附近的必需核酸序列，例如，在聚合酶ii型启动子的情况中，tata元件。启动子还任选地包括远端增强子或阻遏子元件，其可位于距转录起始位点数千碱基对的位置。启动子可以是异源性启动子。

“表达盒”是重组或合成产生的核酸构建体，具有一系列允许特定多核苷酸序列在宿主细胞中转录的特定核酸元件。表达盒可以是质粒，病毒基因组或核酸片段的一部分。通常，表达盒包括待转录的多核苷酸，其操作性地连接至启动子。启动子可以是异源性启动子。在与多核苷酸操作性地连接的启动子的情况中，“异源性启动子”是指不与自然产物中(例如，在野生型生物中)存在的相同多核苷酸如此操作性地连接的启动子。

“多肽”，“肽”和“蛋白质”在本文中可互换使用，指氨基酸残基的聚合物。三个术语全部适用于其中一个或多个氨基酸残基是相应天然产生氨基酸的人造化学模拟物的氨基酸聚合物，以及天然产生的氨基酸聚合物和非天然产生的氨基酸聚合物。如本文所用，该术语涵盖任意长度的氨基酸链，包括全长蛋白质，其中所述氨基酸残基通过共价肽键连接。

“保守修饰的变体”适用于氨基酸和核酸序列。关于特定的核酸序列，“保守修饰的变体”是指那些编码相同或基本相同的氨基酸序列的核酸，或者当核酸不编码氨基酸序列时，指的是基本相同的序列。由于遗传密码的简并性，大量功能相同的核酸编码任何给定的蛋白质。例如，密码子gca、gcc、gcg和gcu都编码氨基酸丙氨酸。因此，在密码子指定丙氨酸的每个位置，该密码子可以改变为所述的任何相应密码子而不改变编码的多肽。这种核酸变异是“沉默变异”，其是保守修饰变异的一种。本文中编码多肽的每个核酸序列也描述了核酸的每种可能的沉默变异。本领域技术人员应认识到，核酸中的每个密码子(除了aug，其通常是甲硫氨酸的唯一密码子，和tgg，其通常是色氨酸的唯一密码子)均可以被修饰以产生功能相同的分子。因此，编码多肽的核酸的每个沉默变异隐含在每个所描述的序列中。

至于氨基酸序列，本领域技术人员应认识到，核酸、肽、多肽或蛋白质序列的单个取代、缺失或添加(其改变、添加或删除编码序列中的单个氨基酸或少量氨基酸)是“保守修饰的变体”，其中所述改变导致被化学上相似的氨基酸取代的氨基酸取代。提供功能上相似氨基酸的保守取代表是本领域熟知的。此类保守修饰的变体是本发明的多态性变体、种间同源物和等位基因的补充且并不排除它们。在一些情况下，保守修饰的cas9或sgrna变体可具有如本文所述的增加的稳定性、组装或活性。

以下八组每组含有相互保守取代的氨基酸：

1)丙氨酸(a)，甘氨酸(g)；

2)天冬氨酸(d)，谷氨酸(e)；

3)天冬酰胺(n)，谷胺酰胺(q)；

4)精氨酸(r)，赖氨酸(k)；

5)异亮氨酸(i)，亮氨酸(l)，甲硫氨酸(m)，缬氨酸(v)；

6)苯丙氨酸(f)，酪氨酸(y)，色氨酸(w)；

7)丝氨酸(s)，苏氨酸(t)；和

8)半胱氨酸(c)，甲硫氨酸(m)

(参见，例如，creighton，《蛋白质》(proteins),弗里曼公司(w.h.freemanandco.)，纽约(1984))。

在本文中，氨基酸可用iupac-iub生物化学命名委员会推荐的通用三字母符号或单字母符号表示。同样，核苷酸可由其公认的单字母代码表示。

在本申请中，氨基酸残基根据它们在未修饰的野生型多肽序列中从最左边的残基(编号为1)的相对位置编号。

如本文所用，在描述两个或更多个多核苷酸或氨基酸序列的上下文中，术语“相同”或“相同性”百分比是指两个或更多个相同的序列或指定的子序列。当比较和比对以在比较窗口上进行最大对应，或使用序列比较算法或通过手动比对和目视检查测量的指定区域(其中未指定特定区域)时，两个“基本相同”的序列具有至少60％的相同性，优选65％，70％，75％，80％，85％，90％，91％，92％，93％，94％，95％，96％，97％，98％，99％或100％相同性。关于多核苷酸序列，该定义还指测试序列的互补序列。关于氨基酸序列，在一些情况下，相同性存在于长度为至少约50个氨基酸或核苷酸的区域上，或更优选地长度为75-100个氨基酸或核苷酸的区域上。

为了序列比较，一般将一个序列用作与测试序列比较的参比序列。当使用序列比较算法时，将测试和参比序列输入计算机，如果需要，指定子序列坐标，并指定序列算法程序参数。可使用默认的程序参数，或者可指定另外的参数。然后，序列比较算法基于程序参数计算测试序列相对于参比序列的序列相同性百分比。对于核酸和蛋白质的序列比较，使用blast2.0算法和下面讨论的默认参数。

如本文所用，“比较窗口”包括对于选自20至600，通常约50至约200，更通常约100至约150个的多个邻接位置中的任何一个的区段的参考，其中，在两个序列最佳比对后，可以将序列与连续位置的相同数目的参比序列进行比较。

用于确定序列相同性百分比和序列相似性的算法是blast2.0算法，其描述于altschul等,(1990)j.mol.biol.215:403-410。用于进行blast分析的软件可在国家生物技术信息中心网站ncbi.nlm.nih.gov上公开获得。此算法包括：首先通过鉴定查询序列中长度为w的短字来鉴定高评分序列对(hsp)，与数据库序列中长度相同的字比对时它们能匹配或满足一些正值的阈值评分t。t称为相邻字评分阈值(altschul等，同上)。这些初始相邻字命中(wordhit)用作启动搜索的种子，以便找到含有它们的较长hsp。然后，沿各序列在两个方向上延伸该字命中，直到提高累积的比对评分。就核苷酸序列而言，采用参数m(一对匹配残基的奖励评分；总是>0)和n(错配残基的罚分；总是<0)计算累积评分。就氨基酸序列而言，用评分矩阵计算累积评分。当以下情况发生时，中止字命中在各方向上的延伸：与最大获得值相比，累积比对评分减少x；由于一个或多个负评分残基比对的累积，累积评分接近零或低于零；或者到达任一序列末端。blast算法参数w、t和x确定该比对的灵敏度和速度。blastn程序(用于核苷酸序列)采用的默认值如下：字长(w)28，期望值(e)10，m＝1，n＝-2，以及比较两条链。就氨基酸序列而言，blastp程序使用默认参数，字长(w)3、期望值(e)10、使用blosum62评分矩阵(参见henikoff和henikoff(1989)procnatlacadsciusa89:10915(1989))。

blast算法也对两个序列间的相似性进行统计学分析(参见例如，karlin和altschul，proc.nat’l.acad.sci.usa90:5873-5787(1993))。blast算法提供的一种相似性度量是最小概率和(p(n))，它表明两条核苷酸或氨基酸序列之间偶尔发生匹配的概率。例如，如果测试核酸与参比核酸比较时的最小概率和小于约0.2，更优选小于约0.01，最优选小于约0.001，那么认为该核酸与参比序列相似。

“crispr/cas”系统是指用于防御外来核酸的一类细菌系统。crispr/cas系统存在于广泛的真细菌和古细菌中。crispr/cas系统包括如下类型：i、ii、iii、v和vi亚型。野生型ii型crispr/cas系统利用rna介导的核酸酶，cas9，与向导和活化rna一起，识别和切割外来核酸。

cas9同源物存在于各种真细菌中，包括但不限于以下分类群的细菌：放线细菌(actinobacteria)，产水菌(aquificae)，拟杆菌-绿菌(bacteroidetes-chlorobi)，衣原体-疣微菌(chlamydiae-verrucomicrobia)，绿弯菌(chlroflexi)，蓝细菌(cyanobacteria)，厚壁菌(firmicutes)，变形菌门(proteobacteria)，螺旋体(spirochaetes)，和热袍菌(thermotogae)。示例性cas9多肽是酿脓链球菌cas9多肽。其它cas9蛋白及其同系物描述于，例如，chylinksi等，rnabiol.2013年5月1日；10(5):726–737；nat.rev.microbiol.2011年6月；9(6):467-477；hou等,procnatlacadsciusa.2013年9月24日；110(39):15644-9；sampson等,nature.2013年5月9日；497(7448):254-7；和jinek等,science.2012年8月17日；337(6096):816-21。cas9蛋白可以是核酸酶缺陷型的。例如，cas9蛋白可以是在靶dna上产生切口但不会导致双链断裂的切口内切核酸酶。cas9还可以使两个核酸酶结构域失活以产生“死亡cas9”(dcas9)，这是一种没有核酸酶活性的可编程dna结合蛋白。在一些实施方式中，dcas9dna结合性由本文所述的多肽抑制。

技术实现要素：

一方面，提供一种抑制细胞中cas9多肽的方法。在一些实施方式中，所述方法包括，

将cas9抑制性多肽引入细胞，其中：该cas9抑制性多肽对于所述细胞而言是异源性的，并且，该cas9抑制性多肽与seqidno:1-170中任一或多个基本(例如，至少60％，70％，80％，90％，95％)相同，由此抑制细胞中的cas9多肽。在一些实施方式中，所述方法包括，使cas9抑制性多肽与细胞中的cas9多肽接触。在一些实施方式中，所述方法包括，使cas9抑制性多肽与所述细胞中crispr-cas9系统的其它组分接触，由此间接抑制cas9多肽活性。

在一些实施方式中，所述cas9抑制性多肽包含seqidno:1-170之一。

在一些实施方式中，在所述引入之前，所述细胞包含cas9多肽。在一些实施方式中，所述细胞包含表达盒，该表达盒含有操作性连接至编码该cas9多肽的多核苷酸的启动子。在一些实施方式中，所述启动子是诱导型的，并且所述方法包括，在所述引入之前，使所述细胞与诱导所述cas9多肽在所述细胞中表达的试剂或条件接触。

在一些实施方式中，在所述引入之后，所述细胞包含cas9多肽。在一些实施方式中，所述启动子是诱导型的，并且所述方法包括，在所述引入之后，使所述细胞与诱导所述cas9多肽在所述细胞中表达的试剂或条件接触。

在一些实施方式中，所述引入包括，在所述细胞中由表达盒表达cas9抑制性多肽，其存在于所述细胞中且对所述细胞而言是异源性的，其中，所述表达盒包含操作性连接至编码所述cas9抑制性多肽的多核苷酸的启动子。在一些实施方式中，所述启动子是诱导型启动子，且所述引入包括使所述细胞与诱导该cas9抑制性多肽的表达的试剂接触。

在一些实施方式中，所述引入包括将编码所述cas9抑制性多肽的rna引入所述细胞，且在所述细胞中从该rna表达该cas9抑制性多肽。

在一些实施方式中，所述引入包括将所述cas9抑制性多肽插入所述细胞或使所述细胞与所述cas9抑制性多肽接触。

在一些实施方式中，所述细胞是真核细胞。在一些实施方式中，所述细胞是哺乳动物细胞。在一些实施方式中，所述细胞是人细胞。在一些实施方式中，所述细胞是血液或诱导型多能干细胞。在一些实施方式中，所述细胞是原核细胞。

在一些实施方式中，所述方法离体进行。在一些实施方式中，在所述引入和接触之后，将所述细胞引入哺乳动物。在一些实施方式中，所述细胞对于所述哺乳动物而言是自体同源的。

还提供了细胞(任选分离的)，其包含cas9抑制性多肽，其中该cas9抑制性多肽对于所述细胞而言是异源性的，并且该cas9抑制性多肽与seqidno:1-170中任一或多个基本相同。在一些实施方式中，所述细胞是真核细胞。在一些实施方式中，所述细胞是哺乳动物细胞。在一些实施方式中，所述细胞是人细胞。在一些实施方式中，所述细胞是原核细胞。

还提供了多核苷酸，其包含编码cas9抑制性多肽的核酸。在一些实施方式中，所述cas9抑制性多肽与seqidno:1-170中任一或多个基本相同。在一些实施方式中，所述多核苷酸包含表达盒，所述表达盒包含操作性连接至所述核酸的启动子。在一些实施方式中，所述启动子对于编码该cas9抑制性多肽的多核苷酸而言是异源性的。在一些实施方式中，所述启动子是诱导型的。在一些实施方式中，所述多核苷酸是dna或rna。

还提供了载体，其包含如上文或本文他处所述的表达盒。在一些实施方式中，所述载体是病毒载体。

还提供了(任选分离的)cas9抑制性多肽。在一些实施方式中，所述cas9抑制性多肽与seqidno:1-170中任一或多个基本相同。

还提供了药物组合物，其包含如上文或本文他处所述的多核苷酸或多肽。

还提供了递送载剂，其包含如上文或本文他处所述的多核苷酸或多肽。在一些实施方式中，所述递送载剂是脂质体或纳米颗粒。

其它方面在本文档的其余部分中描述。

附图说明

图1a-c.单核细胞增生李斯特菌中crispr-cas9基因组自靶向(st)的研究。

图1a.描述基因组自靶向原理的示意图，其中可动遗传元件(mge)具有同一基因组中的crispr阵列中的间隔子的靶序列。这种“自靶向基因组”中的crispr-cas9功能可能对于持续的细胞活力无活性。

图1b.在单核细胞增生李斯特菌基因组中具有cas9连接的自靶向(红色)和没有st(灰色)的基因组的丰度。

图1c.st事件的一个实例，其中菌株j0161的crispr阵列中的间隔子16具有完美pam和前间区序列(与驻留原噬菌体(φj0161a)匹配)(间隔子序列：crrna(seqidno:171)、前间区序列和前间区序列互补序列(seqidno:172-173))。

图2a-d.来自单核细胞增生李斯特菌j0161的原噬菌体包含两个crispr-cas9抑制基因。

图2a.单核细胞增生李斯特菌10403s中的ii-a型crispr-cas基因座。指示了四个cas基因，连同tracrrna和crispr阵列，含有30个间隔子。预期转录方向以黑色箭头指示。后续实验采用非靶向质粒(pnt)和靶向质粒(pt)，其具有与该菌株中间隔子1匹配的前间区序列。

图2b.在crispr-cas-靶向(pt)或非靶向(pnt)质粒经电穿孔进入单核细胞增生李斯特菌10403s的噬菌体治愈型(phage-cured)(φ治愈型)菌株和野生型(wt)j0161(包含φj0161a原噬菌体)之后的转化集落的代表图片，以红色显示以表示自靶向。经分析的φ治愈型10403s变体包括：cas9缺失菌株(δcas9)、φj0161a的溶素原(::φj0161a)、组成型表达来自φj0161a的个体crispr-cas9抑制基因的菌株(+acriia1，+acriia2)和其中crispr-cas9抑制基因缺失的φj0161a的溶素原(::φj0161aδacriia1-2)。图7为wt与φ治愈型10403s之比较。

图2c.评价10403sφ治愈型和wtj0161菌株转化效率。分析了10403sφ治愈型cas9缺失菌株(δcas9)(其组成型表达cas9)(δcas9+cas9)和这些菌株的φj0161a溶素原(::φj0161a)。误差线反映了三个生物学重复的标准偏差。l.d.检测限。

图2d.来自单核细胞增生李斯特菌10403s和j0161的两种相似原噬菌体的开放阅读框之比较。在10403s中测试了包含十个φj0161片段的独特基因(红色)的crispr-cas9抑制。n.e.，对于crispr-cas9活性无作用，tox.，片段在表达时有毒性，t.，自靶向前间区序列的位置。环绕的片段表现出抗crispr活性，其中两个基因(acraii1、acraii2)能够独立地抑制crispr-cas活性。未测试保守(灰色)基因。作为参考，标记了参与细胞裂解、衣壳组装和宿主整合(int.)的噬菌体基因。

图3a-c.基因组组织与acriia基因流行性。

图3a.将acriia1(1)的基因组背景，及其来自单核细胞增生李斯特菌的同源物(orfd)按比例描绘为示意图，其中acriia1同源物垂直对齐。acriia基因一般在原噬菌体内于噬菌体溶素(ply)基因邻近处或附近编码。显示acriia1和orfd的基因组邻居(acriia1-4，orfa-e)。研究单核细胞增生李斯特菌10403s中个体基因(***)的crispr-cas9抑制(参见图9)。使用隐马尔可夫模型(hmm)预测软件在一些蛋白质中检测到螺旋-转角-螺旋(hth)和ap2dna结合基序(j.,biegert,a.和lupas,a.n.(2005).nucleicacidsresearch,33,w244–w248)。

图3b.每个acriia基因共现频率的饼图表示。

图3c.饼图表示单核细胞增生李斯特菌泛基因组中acriia和cas9基因的流行性。相关登录号参见补充表1。

图4a.acriia1-4同源物的进化分析

在迭代psi-blastp检索后鉴定全长蛋白质序列的进化重建以查询genbank内acriia1的所有非冗余蛋白质序列(图4a)。

采用blastp构建(图4b)acriia2，(图4c)acriia3和图4dacriia4(参见方法)的类似树。选定的自举支持值用填充椭圆(≥90％)，空心矩形(≥70％)或虚线(<70％)表示。盒中的序列家族代表了针对抗crispr功能进行测试的家族。其它同源物反映了树下描述的基因组中存在的不同序列家族。

图5a-d.酿脓链球菌(streptococcuspyogenes)dcas9和cas9的抑制。

图5a.概述实验装置的示意图，其中测量了具有酿脓链球菌(spy)dcas9和靶向染色体红色荧光蛋白(rfp)基因的向导rna的大肠杆菌bw25113的单细胞荧光。

图5b.测试候选(orf)和验证的(acr)acriia基因抑制基于dcas9的遏制的能力。所采取的测量结果反映了单峰细胞群中单个细胞的中值rfp荧光值，其中各候选基因针对无向导rna对照进行标准化。误差线表示至少三个生物学重复的标准偏差。基因鉴定信息参见图2和图9。

图5c.概述实验设置的示意图，其中具有多西环素诱型egfp盒的hek293t细胞用编码单一转录物tracrrna/egfp靶向性向导rna和nls-spycas9的质粒以及编码五个密码子优化的噬菌体基因之一的表达构建体以不同比例一起转染。通过流式细胞术检测诱导后12小时的egfp阳性细胞的百分比。

图5d.描绘了egfp阳性细胞的平均百分比+/-标准偏差(三个生物重复)。从左至右添加递增量的抑制质粒(以ng计)，与cas9/sgrna质粒的比例为1:1和3:1。数据对转染标准化，以无噬菌体orf作为基线。

图6是cas9的acriia-抑制的示意图。

图7a-f.crispr-cas9在单核细胞增生李斯特菌j0161中的自靶向与功能丧失型突变无关，与图1相关。

图7a.比较来自酿脓链球菌sf370(spy_sf370)、单核细胞增生李斯特菌10403s(lmo_10403s)、单核细胞增生李斯特菌j0161(lmo_j0161)和无害李斯特菌(listeriainnocua)(lin_clip11262)的ii-a型crispr-cas基因座。显示cas9蛋白质序列之间的相同性百分比。

图7b.自靶向的菌株lmoj0161的crispr阵列。显示由crisprdetect网络实用程序预测的ii-a型crispr阵列(间隔子_序列：分别为seqidno:174-192；重复_序列:seqidno:193)。自靶向的间隔子(编号16(seqidno:189)在框中。粗体为负责靶标识别的rna编码核苷酸。

图7c修饰的crispr靶输出，描述单核细胞增生李斯特菌j0161的自靶向。预测的crrna加工位点由楔形图标标示。(序列：crrna(seqidno:194)，靶dna和靶dna互补序列(seqidno:195-196))。

图7d.tracrrna基因座之比对。(spy_sf370(seqidno:197)；lmo_10403s(seqidno:198)；lmo_j0161(seqidno:199):lin_clip11262(seqidno:200)。

图7e.crispr基因座之比对。(spy_sf370(seqidno:201)；lmo_10403s(seqidno:202)；lmo_j0161(seqidno:203):lin_clip11262(seqidno:204)。

图7f.cas9蛋白质序列之比对。具有基础化学功能性的残基在框内。(spy_sf370(seqidno:205)；lmo_10403s(seqidno:206)；lmo_j0161(seqidno:207):lin_clip11262(seqidno:208)。

图8.φ10403s原噬菌体不影响单核细胞增生李斯特菌10403s中的质粒靶向，图2a-d相关。代表性的平板说明转染和针对靶向(pt；prau31)或非靶向(pnt；prau29)质粒进行选择之后的集落。分析了10403s的野生型(wt)和非溶素原型(φ治愈型)菌株。有关质粒和菌株设计和命名的其它信息，请参见表s1。

图9.在单核细胞增生李斯特菌10403s中对crispr-cas9抑制活性进行筛选的φj0161a原噬菌体的片段，图2相关。代表性平板说明在转化和针对靶向(pt；prau31)或非靶向(pnt；prau29)质粒进行选择之后的集落。按其在图2d中的命名提供所有噬菌体片段的dna序列信息。有关质粒和菌株设计和命名的其它信息，请参见表s1。

图10a-b.单核细胞增生李斯特菌10403s中针对crispr-cas9抑制活性进行筛选的个体基因，图2b和图3a相关。代表性平板说明在转化和针对靶向(pt；prau31)或非靶向(pnt；prau29)质粒进行选择之后的集落。为所有候选ii-a型crispr-cas抑制剂提供给定名称、基因座标签、登录号和dna序列信息。有关质粒和菌株设计和命名的其它信息，请参见表s1。

图11a-b.来自酿脓链球菌的acriia3同源物在大肠杆菌中的毒性，图5相关。

图11a.具有和不具有acriia蛋白表达的大肠杆菌crispri报告菌株的单细胞rfp荧光值的分布。acriia蛋白的表达导致群体荧光向sgrna(无crispri敲减)状态的单峰移位，表明crispri活性的均匀破坏。菌株在iptg存在下生长2.5小时以诱导crispri，其中有或没有acriia抑制剂的表达。

图11b.spyacriia3的表达在大肠杆菌中具有毒性。在存在iptg(crispri诱导)和阿拉伯糖(acriia3诱导)的情况下，spyacriia3在存在或不存在sgrna时是有毒的，表明其毒性不依赖于crispri活性。

图12.ppl2oexl的载体示意图，图2和图3相关。将待测试单核细胞增生李斯特菌10403s中的crispr-cas9抑制的基因和噬菌体片段在phyper和flag标签之间克隆到ppl2oexl中。ppl2oexl衍生物中包括原始终止密码子。

图13：acriia1在厚壁菌中非常普遍，同源物可能会抑制它们所在的生物体中的cas9功能。为了鉴定具有抗crispr功能的acriia基因的新同源物，在基于细胞的测定中测试来自本文所示进化树的不同成员的抗crispr活性。这些已知的抗crispr基因的同源物正在外来细菌系统(铜绿假单胞菌)中进行测试，以鉴定对spycas9具有直接活性的那些基因。

图14：噬菌体噬斑测定显示，对照噬菌体(d3)或由具有jbd30特异性sgrna的spycas9靶向的噬菌体(jbd30)在异源宿主(铜绿假单胞菌)中的十倍稀释。指示的抗crispracriia4(阳性对照)或acriia1的表达使spycas9失活。

图15a-b：15a)不同李斯特菌可动元件中存在的acriia2同源物的多序列比对。(acriia2a.1(seqidno:69)；acriia2a.2(seqiddno:84)；acriia2b.1(seqidno:108)；acriia2b.3(seqidno:209)；acriia2c.1(seqidno:97)；和acriia2c.2(seqidno:98))。测试的同源物用彩色箭头表示，汇总结果显示在比对下方，15b)汇总不同同源物与其登录号之间的序列相同性(氨基酸水平)的表格。

图16：细菌噬菌体噬斑测定，采用十倍连续稀释噬菌体jbd30，在表达spycas9和靶向噬菌体jbd30的sgrna的铜绿假单胞菌菌苔顶部。噬菌体在没有crispr活性的情况下会形成斑块。cas9和sgrna从左至右(0.001％，0.01％，0.1％)与递增量的阿拉伯糖一起诱导。在没有cas9(δcas9)的情况下，噬菌体完全斑块化，但是存在cas9但没有抗-crispr(载体)的情况下，当诱导cas9时，成斑减少。acriia4(阳性对照)的提供在各个水平完全阻止了cas9。只有acriia2b.3与acriia4的活性相当。最初的acriia2a.1仅部分活跃，acriia2b.1略有改善。

图17：测试链球菌属物种中存在的acriia3b的同源物在表达spycas9和sgrna的异源系统(铜绿假单胞菌)中的抗crispr活性。表中显示了结果汇总(图6中的数据)，表明抗crispr的起源物种，cas9在该物种中与酿脓链球菌的序列相同性，以及抗crispr的类似信息。鉴于先前观察到的acriia3a和acriia3b的毒性，评估了这些新蛋白质的毒性(毒性？)和抗crispr功能(acr？)。

图18：细菌细胞在lb琼脂平板上的点滴定(spottitration)。用靶向铜绿假单胞菌基因组的sgrna编程spycas9，从而杀死细胞。如果抗crispr功能正常，细胞才能存活。平板显示10倍连续稀释的细胞铺在非诱导性(左栏)，仅acr诱导性(中间栏，测试acr毒性)，或cas9/sgrna/acr诱导性板(右栏，以测试acr功能)上。由cas9切割的基因组导致死亡，除非抗crispr阻断cas9功能。最右侧板上的菌落指示acr活性。

图19：实施例2中一些数据的总结。

具体实施方式

已从噬菌体鉴定cas9核酸酶的数种多肽抑制剂(“cas9抑制性多肽”)。最初从噬菌体发现的cas9抑制性多肽命名为acriia1，acriia2，acriia3和acriia4。

本文所述的cas9抑制性多肽可用于许多方面以抑制不需要的cas9活性。例如，一种或多种cas9抑制性多肽可用于在基因组编辑中调节cas9，从而在引入cas9抑制性多肽之前允许一些cas9活性。例如，这可能有助于限制cas9的脱靶效应。以下更详细地描述这种和其它用途。

如实施例和序列表中所述，已发现大量cas9抑制性多肽。示例性cas9抑制性多肽的实例包括包含seqidno:1-169中任一者或基本上(例如，至少50，60，70，75，80，85，90，95，或98％)相同氨基酸序列的蛋白质。在一些实施方式中，除了具有上述序列之一外，所述多肽还包括在氨基末端、羧基末端或两者处的其它氨基酸序列或其它化学部分(例如，可检测标记物)。其它氨基酸序列可包括但不限于，标签、可检测标记物或核定位信号序列。

如实施例中注意到的，已显示多种cas9抑制性多肽抑制单核细胞增生李斯特菌cas9以及酿脓链球菌(spy)cas9。据信并预期本文所述的cas9抑制性多肽还将类似地抑制其它阻遏ii-acas9蛋白。本文中所用的“cas9抑制性多肽”是在转化效率测定过程中抑制单核细胞增生李斯特菌中cas9酶功能的蛋白质。当采用带有靶向的dna序列和前间区序列邻近基序(pam)的质粒来转化具有完整cas9功能的菌株时，该转化事件被cas9阻止，在选择下产生微小的集落。将其与具有非靶向dna序列的质粒进行比较，当用于转化单核细胞增生李斯特菌时，其产生正常大小的集落。cas9抑制剂的表达中和cas9活性并导致靶向和非靶向质粒的转化的、正常大小的集落。尽管认为可以其它方式测量cas9抑制性多肽的抑制活性，但是在实施例中更详细地呈现的上述测定是用于确定cas9抑制性多肽是否具有活性的测定。

可以将cas9抑制性多肽引入任何细胞中以抑制该细胞中的cas9。在一些实施方式中，当cas9抑制性多肽被引入细胞时，所述细胞包含cas9蛋白。在其它实施方式中，将cas9抑制性多肽引入细胞，然后将cas9多肽引入所述细胞。

cas9抑制性多肽向细胞中的引入可采取不同形式。例如，在一些实施方式中，将cas9抑制性多肽本身引入细胞。可采用将多肽引入细胞的任何方法。例如，在一些实施方式中，可采用电穿孔或脂质体或纳米颗粒递送至细胞。在其它实施方式中，将编码所述cas9抑制性多肽的多核苷酸引入细胞，且随后cas9抑制性多肽在所述细胞中表达。在一些实施方式中，所述多核苷酸是rna。在一些实施方式中，所述多核苷酸是dna。

在一些实施方式中，cas9抑制性多肽在细胞中由表达盒编码的rna表达，其中该表达盒包含操作性连接至编码所述cas9抑制性多肽的多核苷酸的启动子。在一些实施方式中，所述启动子对于编码该cas9抑制性多肽的多核苷酸而言是异源性的。对于启动子的选择将取决于其中待表达该启动子的细胞以及期望的表达模式。在一些实施方式中，启动子是诱导型或阻抑型启动子，从而操作性连接至该启动子的核酸的表达可在选定条件下表达。在一些示例中，启动子是诱导型启动子，从而操作性连接至该启动子的核酸的表达是被激活或增加的。

诱导型启动子可以通过存在或不存在特定分子来活化，例如多西环素，四环素，金属离子，醇或类固醇化合物。在一些实施方式中，诱导型启动子是由环境条件激活的启动子，例如光或温度。在其它示例中，启动子是阻抑型启动子，从而操作性连接至该启动子的核酸的表达可被减低至低水平或不可检测的水平，或被消除。阻抑型启动子可以通过阻遏物分子的直接结合(例如在色氨酸存在下trp阻遏物与trp操纵子的结合)来抑制。在具体示例中，阻抑型启动子是四环素阻抑型启动子。在其它示例中，阻抑型启动子是由环境条件抑制的启动子，例如缺氧或暴露于金属离子。

在一些实施方式中，通过载体将编码该cas9抑制性多肽的多核苷酸(例如，作为表达盒的部分)递送至细胞。例如，在一些实施方式中，载体是病毒载体。示例性病毒载体可包括但不限于腺病毒载体，腺相关病毒(aav)载体和慢病毒载体。

在一些实施方式中，cas9抑制性多肽或编码所述cas9抑制性多肽的多核苷酸作为细胞递送系统的部分或在细胞递送系统内递送。各种递送系统是已知的，且可用于给予本公开的组合物，例如，脂质体、微颗粒、微胶囊中的包封物，或受体介导的递送。

示例性的脂质体递送方法描述于metselaar等,minirev.med.chem.2(4):319-29(2002)；o'hagen等,expertrev.vaccines2(2):269-83(2003)；o'hagan,curr.drugtargetsinfjct.disord.1(3):273-86(2001)；zho等,bioscirep.22(2):355-69(2002)；chikh等,bioscirep.22(2):339-53(2002)；bungener等,biosci.rep.22(2):323-38(2002)；park,bioscirep.22(2):267-81(2002)；ulrich,biosci.rep.22(2):129-50；lofthouse,adv.drugdeliv.rev.54(6):863-70(2002)；zhou等,j.inmunmunother.25(4):289-303(2002)；singh等,pharmres.19(6):715-28(2002)；wong等,curr.med.chem.8(9):1123-36(2001)；和zhou等,immunonmethods(3):229-35(1994)。

示例性的纳米颗粒递送方法，包括金，氧化铁，钛，水凝胶和磷酸钙纳米颗粒递送方法，描述于wagner和bhaduri，tissueengineering18(1):1-14(2012)(描述无机纳米颗粒)；ding等.，molthere-pub(2014)(描述金纳米颗粒)；zhang等.，langmuir30(3):839-45(2014)(描述二氧化钛纳米颗粒)；xie等.，currpharmbiotechnol14(10):918-25(2014)(描述生物可降解磷酸钙纳米颗粒)；和sizovs等，jamchemsoc136(1):234-40(2014)。

本文所述的cas9抑制性多肽向原核细胞的引入可通过用于将蛋白质或核酸引入原核生物的任何方法来实现。在一些实施方式中，cas9抑制性多肽通过递送载体(例如，细菌噬菌体)递送至原核细胞，该递送载体递送编码所述cas9抑制性多肽的多核苷酸。在一些实施方式中，在原核生物中抑制cas9可协助噬菌体杀伤该细菌或协助其它噬菌体杀伤它。

可将本文所述的cas9抑制性多肽引入包含、表达，或预期表达cas9的任何细胞。示例性的细胞可以是原核或真核细胞。示例性原核细胞可包括但不限于，用于生物技术目的的那些，期望代谢物的生产，大肠杆菌和人病原体。此类原核细胞的示例可包括，例如，大肠杆菌(escherichiacoli)，假单胞菌(pseudomonassp.)，棒状杆菌(corynebacteriumsp.)，枯草芽孢杆菌(bacillussubtitis)，肺炎链球菌(streptococcuspneumonia)，绿脓假单胞菌(pseudomonasaeruginosa)，金黄色酿脓葡萄球菌(staphylococcusaureus)，空肠弯曲杆菌(campylobacterjejuni)，新凶手弗朗西丝氏菌(francisellanovicida)，白喉杆菌(corynebacteriumdiphtheria)，肠球菌(enterococcussp.)，单核细胞增多性李斯特氏菌(listeriamonocytogenes)，鸡毒支原体(mycoplasmagallisepticum)，链球菌(streptococcussp.)，或齿垢密螺旋体(treponemadenticola)。示例性的真核细胞可包括，例如，动物(例如，哺乳动物)或植物细胞。示例性的哺乳动物细胞包括但不限于人，非人灵长类，小鼠，和大鼠细胞。细胞可以是培养细胞或原代细胞。示例性的细胞类型可包括但不限于，诱导型多能细胞，干细胞或祖细胞，和血细胞，包括但不限于t-细胞或b-细胞。

在一些实施方式中，从动物(例如，人，任选地需要基因修复的人)中移出细胞，然后将用于基因编辑的cas9和任选向导rna离体引入细胞中，并将cas9抑制性多肽引入细胞中。在一些实施方式中，随后将该一种或多种细胞引入同一动物(自体同源)或不同动物(同种异体)。

在本文所述的任一实施方式中，可将cas9多肽引入细胞以允许cas9dna结合和/或切割(且任选编辑)，随后引入本文所述的cas9抑制性多肽。因此，可通过对该细胞后续提供cas9抑制性多肽由此使cas9失活来控制细胞中存在活性cas9的时机。例如，这可用于减少cas9“脱靶”效应，从而结合或改变非靶向染色体序列。通过将cas9活性限制为在引入cas9抑制性多肽时结束的有限“突发”，可以限制脱靶效应。在一些实施方式中，cas9多肽和cas9抑制性多肽由不同的诱导型启动子表达，受不同诱导物调节。这些实施方式允许首先启动cas9多肽的表达，随后诱导cas9抑制性多肽，任选地同时除去cas9表达的诱导物。

在一些实施方式中，可以将本文所述的cas9抑制性多肽引入(例如，给予)至动物(例如人)或植物。这可以用于控制体内cas9活性，例如在体内进行crispr/cas9基因编辑的情况下，或者在个体暴露于不需要的cas9的情况下，例如在释放包含cas9的生物武器的情况下。

在一些实施方式中，cas9抑制性多肽或编码所述cas9抑制性多肽的多核苷酸作为药物组合物给予。在一些实施方式中，所述组合物包含递送系统如脂质体、纳米颗粒或本文所述或其它情况下已知的其它递送载剂，其包含所述cas9抑制性多肽或编码所述cas9抑制性多肽的多核苷酸。可以使用本领域已知的任何途径将组合物直接施用于哺乳动物(例如人)以抑制cas9，包括例如通过注射(例如，静脉内，腹膜内，皮下，肌肉内或内部)，吸入，透皮施用，直肠给药或口服给药。

本发明的药物组合物可包含药学上可接受的运载体。药学上可接受的运载体部分地由所给予的特定组合物以及用于给予组合物的特定方法确定。因此，存在多种合适的本发明药物组合物制剂(参见例如，《雷明顿药物科学》(remington’spharmaceuticalsciences)，第17版，1989)。

实施例

实施例1

结果

crispr-cas9在单核细胞增生李斯特氏菌中靶向外来dna

单核细胞增生李斯特氏菌是一种兼性细胞内食源性病原体，具有良好表征的噬菌体群体。许多单核细胞增生李斯特菌分离株具有ii-a型crispr-cas系统(sesto,n.等.(2014).plosgenet,10,e1004065)且其crispr间隔子与许多许多毒性、温和与整合型噬菌体具有相同性(di,h.等.(2014)biochembiophysrescommun,454,399–403；sesto,n.等.(2014).plosgenet,10,e1004065)。然而，对于经典crispr-cas功能缺乏实验证据。我们分析了单核细胞增生李斯特菌的275个基因组，并且在15％(n＝41)的基因组中鉴定了ii-a型crispr-cas9系统(lmocas9)(图1b)。有趣的是，我们发现八个基因组(总数的3％)，具有自靶向的例子(图1b和1c)，尽管预期crispr-cas9可作为crispr重复序列，但pam、tracrrna、cas9和相关启动子缺乏明显的失活突变(图6a-e)。我们预测这些菌株具有crispr-cas9的抑制剂，其允许间隔子-前间区序列对的稳定共存。

为了测试抑制剂是否由单核细胞增生李斯特菌的原噬菌体编码，我们首先在不表现自靶向的单核细胞增生李斯特菌菌株(10403s)中建立了未抑制的crispr-cas9功能。为了测试该系统的活性，我们设计了一种质粒(pt)，其具有靶向的前间区序列(即与crispr阵列中的天然间隔子互补的序列)以及同源的前间区序列相邻基序(pam)，这是一种三碱基基序，是cas9结合所必需的(图2a)。我们用pt或具有相同质粒骨架(pnt)的非靶向序列的对照质粒检测了10403s的转化效率。为了简化下游分析，将10403s的原噬菌体治愈形式(φ治愈型)用于所有下游实验，因为它与该测定中的wt10403s无法区分(图7)。用pt转化产生相对于pnt的微小集落(图2b，最左边的组)，尽管在延长孵育时出现的集落数是相同的(图2c，进一步分析参见讨论)。为了证实该表型是crispr-cas9干扰的结果，我们构建了cas9缺失型菌株。用pt和pnt转化该菌株产生了大小难以区分的集落(图2b左起第二幅图)。然而，在组成型活性启动子控制下将cas9加回到单核细胞增生李斯特菌染色体中完全抑制了用pt的转化(图2c)。总之，这些实验证明cas9在单核细胞增生李斯特菌菌株10403s中，在内源性和过表达水平上都是有功能的，并且限制了用带有前间区序列的质粒的转化。

驻留原噬菌体使单核细胞增生李斯特菌中的crispr-cas9失活

为了确定在具有自靶向间隔子的菌株中可否使crispr-cas9失效，我们检测了单核细胞增生李斯特菌菌株j0161中的免疫功能，其间隔子16与同一基因组中的原噬菌体(φj0161a)完全匹配(图1c)。我们无法在j0161中检测到任何明显有害的crispr-cas突变，这表明这种自靶向情况是抑制而非功能丧失的结果(图6b-f)。由于j0161的ii-a型crispr阵列与10403s不同，因此使用j0161特异性靶向质粒(ptj0161)来测试j0161中的crispr-cas9功能。与自靶向所暗示的失活一致，转化效率或集落大小没有区分ptj0161和pnt(图2b和2c，最右边的组)的显著差异。因此，我们推断j0161基因组可编码cas9抑制剂。

为了寻找j0161中crispr-cas9失活的遗传基础，我们将重点放在原噬菌体φj0161a上作为抑制基因的可能来源，因为它在该菌株中含有自靶向序列。为了确定φj0161a是否含有抑制剂，将10403s的原噬菌体治愈型菌株用φj0161a溶原化，并通过质粒转化测定crispr-cas9功能性。虽然φ治愈型10403s菌株靶向pt，但φj0161a的获得足以使crispr-cas9功能失活(图2b，左起第3组和2c，左起第4组)，表明该原噬菌体编码crispr-cas9的抑制剂。φj0161a原噬菌体还在组成型表达cas9的菌株中使质粒靶向失活，表明抑制机制不通过破坏cas9启动子的天然调节而起作用(图2c，左起第5幅)。

鉴于φj0161a抑制crispr-cas9功能，而内源性原噬菌体φ10403s不抑制，我们比较了这两个密切相关的噬菌体的基因组以鉴定差异区域(图2d)。除了共享39个具有>40％蛋白质序列相同性的核心噬菌体基因以外，还鉴定了10个非重叠的独特基因簇(基于预测的操纵子结构选择簇边界，每簇1-12个基因)。克隆每个簇并整合到原噬菌体治愈型10403s的基因组中并测定crispr-cas9功能。在10个片段中，7个成功地引入单核细胞增生李斯特菌，而3个片段不能插入到单核细胞增生李斯特菌基因组中，并且可能在分离时具有毒性。质粒转化测定显示φj0161a片段1是唯一能够抑制crispr-cas9的片段，表明该片段编码至少一种crispr-cas9抑制剂(图2d，图8)。表达来自该四基因片段的个体基因导致两个抗crispr基因lmog_03146和lmog_03147的确定鉴定(本文分别称为acriia1和acriia2；图2b和2d)。10403s::φj0161a溶素原中acriia1和acriia2的缺失恢复了crispr-cas9功能，证实这些是φj0161a中仅有的抗crispr基因(图2b)。

抗crispr基因座在单核细胞增生李斯特菌中广泛存在

为了鉴定其它ii-a型抗crispr，检测了相关的单核细胞增生李斯特菌原噬菌体中与acriia1和acriia2类似的基因组位置。在留下的整合位点和参与细胞裂解的基因之间鉴定出在具有高度保守的基因顺序的小操纵子(2-5个基因)内含有acriia1的重复的抗crispr(acr)基因座(图3a)。我们鉴定了acr基因座内保守的5个新蛋白质家族。为了测试这些，我们将代表物质克隆并整合到10403s基因组中并测定pt和pnt的转化效率。鉴定了两种能够使crispr失活的新基因(acriia3和acriia4)，而其余三种(orfc，d，e)则不是(图3a，图9)。

为了确定单核细胞增生李斯特菌中crispr-cas9的失活是否普遍存在，我们接下来分析了每种抗crispr的保守模式。虽然每个acriia基因足以使分离物中的crispr-cas9失活，但我们观察到在大多数acr基因座中acriia1的常见存在。在含有螺旋-转角-螺旋(hth)基序的acriia1的上游发现几乎所有实例(91％)的acriia2-4，表明该基因可能是acr基因座的标志物(图3b)。我们在119个acr基因座中观察到的最常见的情况是acriia1-2或acriia1-2-3，代表66％的acr基因座。总共在25％的单核细胞增生李斯特菌基因组中鉴定出acriia基因，其中约50％的含有单核细胞增生李斯特菌cas9的菌株在相同的基因组中具有至少一个抗crispr(图3c)。还鉴定了许多具有多种acriia编码原噬菌体的单核细胞增生李斯特菌基因组的实例(补充表1)。此外，在最初鉴定的所有八个自靶向实例的基因组中发现至少一个acriia基因(图1b，补充表1)，解释了这些情况如何稳定。总之，这些数据表明在单核细胞增生李斯特菌中普遍存在原噬菌体介导的crispr-cas9失活。

表s1.acriia基因保守性，图1b4b和4c相关

先前含有hth的抗crispr相关(aca)基因被用作鉴定新型i型抗crispr基因的标志物(pawluk,a.等.(2016).naturemicrobiology,1,1–6)，尽管aca基因本身没有抗crispr活性。我们假设acriia1可以发挥这种标志物的作用。对acriia1进行了全面的进化分析，揭示了在厚壁菌中在可动元件和核心基因组中广泛检测到的同源物(图4a)。在李斯特菌基因组中鉴定了一系列远缘相关的acriia1同源物，例如orfd，其是已经独立鉴定为acr基因座成员的含hth的基因(图3a)。虽然该基因在功能测定中缺乏抗crispr活性，但其与质粒plmiv中的acriia4的共同出现表明广泛的acriia1/orfd超家族可用作鉴定新acr基因的标志物(图3a)。未来的工作对于确定这些系统中含有hth的基因是否可作为新型抗crispr发现的有效标志物是必要的。

为了确定acriia2-4的同源性，进行了类似的进化分析。与在厚壁菌核心基因组中广泛存在的acriia1不同，这些其它三种acr基因大多局限于李斯特菌中的原噬菌体。鉴定了三个不同的acriia2序列家族，仅在李斯特菌长尾噬菌体(siphophages)(一个长尾，非收缩噬菌体家族)中发现(图4b)，而在李斯特菌和链球菌的长尾噬菌体基因组中观察到两个acriia3家族(图4c)。最后，在两个不同的序列家族中观察到acriia4，一个在李斯特菌长尾噬菌体和质粒中，另一个在一组专性毒性噬菌体(长收缩尾噬菌体)中观察到(图4d)。虽然acriia2和acriia3几乎总是与acriia1一起发现，但acriia4经常在缺乏李斯特菌的可动元件和噬菌体中的acriia1同源物时发生。例如，毒性噬菌体中的acriia4家族是不同的，因为它们在预测的蛋白质中具有约70个氨基酸的c末端延伸，并且不与含有hth的基因acriia1或orfd一起出现，表明这些acriia4家族之间存在潜在的机制和进化差异。总之，这些分析揭示了用于检测同源性acr基因的特异性决定因子的充足序列空间，并且提示了在单核细胞增生李斯特菌中cas9和可动元件之间的活跃的竞争。

acriia2、acriia3和acriia4抑制酿脓链球菌cas9

为了确定lmocas9acriia蛋白的多功能性，我们研究这些抑制剂对来自酿脓链球菌的相关cas9蛋白是否起作用(spy，与lmocas953％相同)。该直系同源物已被广泛用于生物技术应用，作为rna引导的核酸酶(barrangou,r.和doudna,j.a.(2016),naturebiotechnology,34,933–941)，以及用于通过用于可编程基因抑制的催化失活的突变体(dcas9)进行的可编程基因抑制(gilbert,l.a.等.(2013).cell154,442-451；qi,l.s.等.(2013).cell,152,1173–1183)。使用携带spydcas9的大肠杆菌菌株，我们测试了acriia蛋白是否阻断dcas9干扰染色体rfp报告基因的转录(图5a)。在缺乏抑制剂的遗传背景中，相对于没有向导rna的菌株，sgrna和dcas9的存在将rfp荧光降低至2.6％。acriia2部分阻断dcas9功能，荧光上升至25％，而acriia4几乎完全阻断dcas9，具有无导向对照的荧光的85％(图5b)。我们无法从acriia3获得有意义的数据，因为该蛋白质对大肠杆菌有毒。这在流式细胞术期间使记录的细胞计数降低(参见图10a)并导致荧光测量的较大变异性。还测试了来自酿脓链球菌的acriia3的同源物(登录号：and04610.1)，其与lmo_acriia3具有45％的序列相同性，但导致大肠杆菌的生长受损(图10b)。acriia3毒性的机制仍有待确定。我们得出结论，acriia2和acriia4在不同程度上抑制大肠杆菌中的spydcas9。

鉴于spycas9在真核细胞中的普遍应用，我们接下来测试了acriia蛋白在人体细胞中阻断基因编辑的能力。在存在或不存在表达人密码子优化的acriia蛋白的载体的情况下，用表达spycas9和靶向egfp的sgrna的质粒瞬时转染具有诱导型egfp报告基因的hek293t细胞。在允许基因编辑进行36小时后，诱导egfp12小时，然后通过流式细胞术测量细胞荧光(图5c)。在cas9和egfpsgrna存在下，基因编辑导致gfp阳性细胞数量减少25％，而与acriia2或acriia4的共表达阻止了基于cas9的基因编辑(图5d)。我们在该测定中另外测试了acriia3(spy_acriia3)的酿脓链球菌同源物，其在人细胞中无毒，但对cas9功能没有影响。acriia1在人细胞中是无功能的，orfa也是，其为一种在单核细胞增生李斯特菌中没有抗crispr活性的阴性对照。与大肠杆菌中的dcas9实验一起，这些数据证明了acriia2和acriia4蛋白在异源宿主中抑制直系同源cas9的功能的效用。因此，这些试剂代表crispr-cas9基因组工程改造工具包中的新工具。

讨论

噬菌体编码的细菌免疫系统抑制剂由于这两个实体之间的进化竞争中的强选择压力而出现(samson,j.e.等.(2013).natrevmicro,11,675–687)。i型crispr-cas系统中噬菌体编码的抗crispr的首次鉴定暗示存在更多的crispr抑制剂，但缺乏其发现的方法。本文中，我们提出了一种生物信息学策略，该策略使用“自靶向”作为crispr-cas抑制剂基因的基因组标志物(图1a)。该方法导致鉴定出四种不同的ii-a型crispr-cas9抑制剂(图3a)，它们共同存在于所有编码cas9的单核细胞增生李斯特菌基因组的一半中，包括具有自靶向的所有基因组(图3c)。我们预计这种方法将有助于鉴定其它crispr-cas系统中的acr基因，尽管已经描述了iii型系统的自靶向耐受的独特机制(goldberg,g.w.等.(2014).nature514,633–637；samai,p.等.(2015).cell,161,1164–1174)。

为了便于鉴定acriia蛋白，我们首先在单核细胞增生李斯特菌中证明了功能性crispr-cas9系统(图2b)。先前对该生物体中crispr-cas的研究集中于ib型系统和缺乏cas基因的相关ib衍生的crispr孤儿阵列(mandin,p.等.(2007).nucleicacidsresearch,35,962–974；sesto,n.等.(2014).plosgenet,10,e1004065)。虽然之前没有为这两种系统建立规范的crispr-cas功能，但是显示孤儿阵列由宿主核糖核酸酶处理以产生非编码rna(mandin,p.等(2007).nucleicacidsresearch,35,962–974；sesto,n.等(2014).plosgenet,10,e1004065)。为了观察ii-acrispr-cas系统的功能，我们使用标准转化效率测定法，显示菌株10403s中的cas9功能足以限制质粒以序列特异性方式转化(图2a-c)。鉴于在用pt转化10403s期间观察到的小集落表型，我们怀疑内源性cas9表达水平不足以完全清除质粒，导致质粒保持在微小的集落中。确认这一点，cas9的表达增加导致该测定中转化体完全消除(图2c)。鉴于φj0161a可以抑制这种过表达的crispr-cas9系统(图2c)，我们得出结论，鉴定的抑制剂能够阻断内源性和过表达的cas9功能。

为了鉴定候选抗crispr基因，比较了来自crispr活性菌株10403s的相关原噬菌体与抑制来自菌株j0161的crispr的原噬菌体，并且采用了消除克隆方法的操作(图2d)。确定了存在于φj0161中的acriia1和acriia2基因的两个分离的acr基因的鉴定。在寻找更多抗crispr中，我们发现相关噬菌体中保守的基因组定位是鉴定不同ii-a型cas9抑制蛋白的良好代表，尽管蛋白质本身之间缺乏序列保守性(图3a)。先前已在if和ie型抗crispr的研究中观察到这种情况(bondy-denomy等(2013).nature,493,429–432；pawluk,a.等(2014).mbio5,e00896)。在单核细胞增生李斯特氏菌中，原噬菌体中cas9抑制剂的高流行性表明crispr-cas9功能的广泛失活(图3c)。目前，我们不了解是否存在机制关联来解释acriia1与其它抗crispr的常见共存(图3a和3b)。尽管该基因足以在质粒攻击测定中使crispr-cas9功能失活，但我们推测其可在感染或溶原过程中充当其它acriia基因的辅因子或调节因子，因此解释了观察到的基因组关联。未来的工作将有必要了解acriia1实际上是否是这方面的双功能蛋白，更广泛地说，超家族是否是acr基因的标志物。

进化分析证明了李斯特菌可动元件中可动元件中acriia2-4的常见发生(图4)。这可能通过阻断基于cas9的靶向和适应来促进该生物体中的水平基因转移(heler,r.等.(2015)nature519,199–202)。除了首次鉴定出这些acriia基因的原噬菌体家族外，还在较远的长尾噬菌体、肌尾噬菌体(myophage)和质粒中发现了同源物。最值得注意的是，由强毒性肌尾噬菌体编码的acriia4同源物在其附近没有acriia1超家族同源物。此外，在李斯特菌属之外的属中存在acriia1和acriia3同源物表明crispr-cas9失活可能在厚壁菌门中是常见的。

为了使crispr-cas9功能失活，理论上许多机制都是可能的。通过证明acriia2和acriia4在具有工程改造的元件(即cas9启动子，sgrna设计和启动子)的异源宿主中的功效，我们得出结论，转录抑制是不可能的。i型抗crispr的功能是通过直接结合干扰所需的cas蛋白并阻止dna结合或dna切割(bondy-denomy,j等.(2015).nature,526,136–139)。通过扩展，我们期望acriia2和acriia4具有类似的机制，因为它们具有在人细胞中发挥作用的能力。相对于基于dcas9的测定，acriia2在人细胞中基于裂解的cas9测定中增强的功效表明它可以在一定程度上抑制结合和切割，其中切割抑制表现为cas9功能的完全失活。在acriia4的情况下，dna结合明显被抑制，尽管这是否通过与cas9的直接相互作用仍有待观察。

ii-a型抑制剂的鉴定和未来机制解析将为在自然和工程化环境中研究经典crispr-cas9功能提供有价值的新试剂。acriia蛋白在大肠杆菌和人细胞中阻断spycas9的能力表明这些蛋白质可以为cas9提供翻译后“关闭”。这可以为这个功能强大的系统增加一层调节，可以应用于真核系统来控制基因组工程改造。crispr-cas9工具箱的这一新增功能可以实现新的应用，例如特异性逆转dcas9与基因组基因座结合的影响，或限制cas9在细胞核中活化的时间量，以减少脱靶基因编辑。重要的是扩大对抑制蛋白的搜索，以继续利用从噬菌体-细菌竞赛中提供给我们的丰富工具。

表s2.菌株与质粒,

实验模型与主题详细信息

微生物

单核细胞增生李斯特菌菌株在脑心浸液(bhi)培养基上培养。大肠杆菌菌株在lb培养基上培养。

细胞系

人胚胎肾293加t细胞抗原(hek293t，crl-3216，atcc)细胞在补充有10％胎牛血清(fbs，atlantabiologicals)和50μg/ml青霉素/链霉素(p/s，ucsfccf)的杜氏改良伊氏培养基(dmem)中培养。

实施例2

我们试图鉴定acriia1-4(图13)的同源物，其具有针对cas9的酿脓链球菌直系同源物的抗crispr功能，其广泛用于基因编辑。

图14：acriia1在异源系统(即天然生物体外)中具有抗cas9活性。以前，该蛋白质在大肠杆菌或人类细胞中不具有抗cas9功能。这种差异的原因尚不清楚，但与阳性对照acriia4相比，acriia1在这种异源系统(铜绿假单胞菌)中的功能非常好，同时靶向cas9。

图15：通过序列比对鉴定acriia2同源物，以广泛地采样天然序列空间。除了原始蛋白质之外，还测试了三种同源物，并且如图16中的噬菌体噬斑测定所示：与原始蛋白质acriia2a相比，acriia2b.1和acriia2b.3具有强的抗spycas9活性。

图17：进行类似的同源性检索以鉴定acriia3同源物，acriia3b.2,3b.3,3b.4。如图18所示，采用其中细菌点在平板上的测定，这三种新的抗crispr蛋白质无毒并且在细菌中稳健有效。如本文所鉴定的，acriia4是一种强大且广谱的cas9抑制剂，其与cas9紧密结合(参见，dong等，nature，和shin等，scienceadvances)。鉴定出一种同源物(acriia4b)，其具有中度的抗cas9活性。

图19汇总了实施例2中所述的结果。

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应理解，本文所述的实施例和实施方式仅用于说明目的，本领域技术人员应了解据此作出的各种修饰或改变，且它们包括在本申请的主旨和权益以及所附权利要求书的范围内。本文引用的所有发表物、专利和专利申请通过引用全文纳入本文以用于所有目的。

序列

acriia1蛋白序列

seqidno:1

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seqidno:2

>wp_060571535.1

mtiklldeflkkhdltryqlskltgisqntlkdqnekplnkytvsilrslslisglsvsdvlfeledieknsddlagfkhlldkyklsfpaqefelyclikefestnievlpftfnrfeneehvnikkdvckalenaitvlkekknell

seqidno:3

>wp_070783094.1

mtiklldeflkkhdltryqlskltgisqntlkdqnekplnkytvsilrslslisglsvsdvlfeledieknsddlagfkhlldkyklsfpaqefelyclikefesnievlpftfnrfeneehvnigkdvckalenaitvlkekknell

seqidno:4

>wp_031669445.1

mtiklldeflkkhdltryqlskltgisqntlkdqnekplnkytvsilrslslisglsvsdvlfeledieknsddlagfkhlldkyklsfpaqefelyclikefesanievlpftfnrfeneehvniekdvckalenaitvlkekknell

seqidno:5

>wp_070286809.1

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seqidno:6

>wp_070213372.1

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seqidno:7

>wp_003731275.1

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seqidno:8

>wp_010989942.1

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seqidno:9

>wp_070286796.1

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seqidno:10

>wp_038409766.1

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seqidno:11

>wp_060595919.1

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seqidno:12

>wp_047934203.1

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seqidno:13

>yp_009044824.1

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seqidno:14

>wp_061396064.1

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seqidno:15

>wp_014930689.1

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seqidno:16

>wp_061105218.1

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seqidno:17

>wp_070216262.1

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seqidno:18

>wp_070761486.1

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seqidno:19

>wp_070005110.1

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seqidno:20

>wp_070784182.1

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seqidno:21

>wp_070776459.1

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seqidno:22

>wp_003737351.1

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seqidno:23

>wp_010991654.1

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seqidno:24

>wp_070295945.1

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seqidno:25

>wp_061662200.1

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seqidno:26

>wp_061665494.1

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seqidno:27

>wp_061107167.1

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seqidno:28

>wp_070005290.1

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seqidno:29

>wp_039385152.1

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seqidno:30

>wp_015967154.1

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seqidno:31

>wp_069001242.1

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seqidno:32

>wp_070040173.1

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seqidno:33

>wp_068999238.1

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seqidno:34

>wp_070784648.1

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seqidno:35

>wp_070777825.1

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seqidno:36

>wp_047934326.1

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seqidno:37

>wp_003727802.1

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seqidno:38

>wp_003723291.1(lmog_02992)

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seqidno:39

>wp_069027465.1

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seqidno:40

>wp_047933338.1

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seqidno:41

>wp_060579665.1

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seqidno:42

>wp_031646274.1

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seqidno:43

>wp_070776287.1

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seqidno:44

>wp_031645842.1

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seqidno:45

>wp_046376633.1

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seqidno:46

>wp_070242402.1

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seqidno:47

>wp_047923954.1

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seqidno:48

>wp_031668927.1

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seqidno:49

>wp_012581438.1

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seqidno:50

>wp_031667947.1

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seqidno:51

>wp_061107116.1

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seqidno:52

>wp_070783481.1

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seqidno:53

>wp_060577773.1

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seqidno:54

>wp_039389295.1

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seqidno:55

>wp_070295880.1

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seqidno:56

>wp_061399219.1

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seqidno:57

>wp_061112070.1

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seqidno:58

>wp_070779352.1

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seqidno:59

>wp_014930929.1

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seqidno:60

>wp_069001897.1

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seqidno:61

>wp_070784143.1

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seqidno:62

>efs02359.1

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seqidno:63

>wp_061128861.1

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seqidno:64

>kug37233.1

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seqidno:65

>wp_049955951.1

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seqidno:66

>wp_009917642.1

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seqidno:67

>wp_070777879.1

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seqidno:68

>wp_061662201.1

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acriia2蛋白序列

seqidno:69

>wp_003722517.1

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seqidno:70

>wp_031668925.1

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seqidno:71

>wp_031646276.1

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seqidno:72

>ezh71062.1

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seqidno:73

>wp_061662199.1

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seqidno:74

>wp_070026783.1

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seqidno:75

>wp_068996202.1

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seqidno:76

>wp_009928183.1

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seqidno:77

>wp_014930690.1

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seqidno:78

>wp_061394923.1

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seqidno:79

>wp_069001241.1

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seqidno:80

>wp_039389299.1

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seqidno:81

>wp_039385155.1

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seqidno:82

>wp_003733874.1

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seqidno:83

>wp_070294198.1

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seqidno:84

>wp_031649390.1

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seqidno:85

>efr93689.1

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seqidno:86

>wp_070295879.1

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seqidno:87

>wp_070776458.1

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seqidno:88

>wp_070005111.1

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seqidno:89

>wp_023553814.1

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seqidno:90

>wp_031645843.1

mtittaqkryydamnefeaitskeleqtpefsqdllndsdylvitkneayavalcmldddklyidetlvqstcldvegetyyinfvvtneddfklatdkdkekhdkqeviikseln

seqidno:91

>wp_014930930.1

mtlttaqkryydamnefeaiiskeleqtrafsqdllndsdylvitkneayavdlcmldddklyldetlvqstrldiedetyyinfvvtneddfklatdedkekhdrqeviikseln

seqidno:92

>wp_070783480.1

mtittaqkryydamnefeaitskeleqtpefsqdllndsdylvvtkneayaaalcmldddklyldetlvqstcldvegeiyyinfvvtneddfklatdkdkekhdkqevivkseln

seqidno:93

>wp_070040172.1

mtittaqkryydamnefeaitskgleqtpefsqdllndfdylvitkneayaaalcmlddeklyldetlvhstrldieddtyyinfvvtneddfklatdedkekhdkqeviikreln

seqidno:94

>wp_012581437.1

mtittaqkryydamnefeaiiskeleqtpafsqdllndsdylvitkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqeviikseln

seqidno:95

>wp_061107168.1

mtittaqkryydamnefeaiiskeleqtpafsqnllndsdylvitkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqeviikseln

seqidno:96

>wp_070243058.1

mtittaqkryydamnefeaitskeleqtpafsqdllndsdylvitkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqevivkseln

seqidno:97

>wp_015967155.1

mtittaqkryydamnefeaiiskeleqtpafsqdllndsdylvitkneayavalclldddklyldetlvhstrlniedetyyinfvvtneddfklatdedkekhdkqeviiksefn

seqidno:98

>wp_010989941.1

mtlttaqkryydamnefeaitskeleqtpefsqdllndsdylavtkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqeviiksgln

seqidno:99

>wp_010991653.1

mtvttaqkryydamnefeaitskeleqtpefsqdllndsdylavtkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqeviikseln

seqidno:100

>wp_068996515.1

mtlttvqkryydamnefeaitskeleqtpefsqdllndsdylavtkneayavalclldddklyldetlvhstrfdiedetyyinfvvtneddfklatdedkekhdkqeviikseln

seqidno:101

>wp_047934322.1

mtlttvqkryydamnefeaitskeleqtpefsqdslndsdylavtkneayavalclldddklyldetlvhstrfdiedetyyinfvvtneddfklatdedkekhdkqeviikseln

seqidno:102

>wp_003727803.1

mtittaqkryydamnefeaiiskeleqtpafsqdllndsdylavtkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqeviiksgln

seqidno:103

>wp_061114505.1

mtittaqkryydamnefeaitskeleqtpafsqdllndsdylavtkneayavalclldddklyldetlvhstrldiedetyyinfvvtneddfklatdedkekhdkqeviiksgln

seqidno:104

>efs02358.1

maitlsqrkfyeainefeemtenevvtspripqdylndgdyvvitksenyalnlcttnlegfedrhfldekliystfvetysgetyyiyitqtaefdeddaveflatqeqiyeyhkqeeqktvilkmels

seqidno:105

>wp_069001896.1

maqteaqkifyeainefeemtneevvtspripqdylndgdyvvitksenyalnlcttdlegfedryfldekliystsvetytdetyyiyitqttefeednaveflatqeqiyeyhkqeeqktvilkmels

seqidno:106

>wp_061665680.1

mttarkkfyqaisefeamtgkdvertpqiadevlndaeyiaftktekyalylctsnvegledryfldeecldstfletednetyyihflqetefseddnedelplateeqieaydkqeelkavilkkeln

seqidno:107

>wp_009917643.1

mrttaqerldnainefeeitneevvtspripqdylndgdyvvitksenyalnlcttnlegfedrhfldekliystfvetysgetyyiyitqtaefdeddaveflatqeqiyeyhkqeeqktvilkmels

seqidno:108

>wp_061112069.1

mrttaqerldnainefeeitneevvtspripqdylndgdyvvitksenyalnlcttnlegfedrhfldekliystfvetyagetyyiyitqtaefdeddaveflatqeqiyeyhkqeeqktvilkmels

seqidno:109

>wp_003745974.1

mrttaqerldnainefeeitneevvtsplipqdylndgdyvvitksenyalnlcttnlegfedrhfldekliystfvetysgetyyiyitqtaefdeddaveflatqeqiyeyhkqeeqktvilkmels

acriia3蛋白序列

seqidno:110

>wp_014930691.1(李斯特菌)

mfnkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveeskkeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:111

>wp_068996201.1

mynkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveeskkeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:112

>wp_031646277.1

mynkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkgawskakeeveeskkeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:113

>wp_003727804.1

mynkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveeskkeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglslwacamkavklhndlfpqtaa

seqidno:114

>wp_070776457.1

mynkaeimkqawnwfndsnvwlsdiewisytdkektfsvclkaawskakeeveeskkeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:115

>wp_015455142.1

mynkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveeskkeskyiakseelkawnwaerklglrfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:116

>wp_070005112.1

mfnkaeimkqawnwftdsnvwlsdiewasytdkeksfsvclkaawskakeeveeskkeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:117

>wp_068996392.1

mynkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveefkkeskyiakseelkawnwaerklglrfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:118

>cuk89695.1

mkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveeskkeskyiakseelkawnwaerklglrfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:119

>wp_060577772.1

mynkaeimkqawnwfnnsnvwlsdiewvsytdkektfsvclkaawskakeeveeskeeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:120

>wp_070295878.1

mynkaeimkqawnwfnnsnvwlsdiewvsytdkektfsvclraawskakeeveeskeeskhiakseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:121

>wp_061396065.1

mfnkaeimkqawnwftdsnvwlsdiewvsytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:122

>wp_039389302.1

mynkaeimkqawnwfndsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:123

>wp_047934319.1

mfnkaeimkqawnwftdsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:124

>wp_069001240.1

mynkaeimkqawnwftdsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:125

>wp_010991652.1

mfnkaeimkqawnwftdsnvwlsdiewvsytdkektfsvclkaawskakeefkevekeikhiskseelkawnwaerklglhfnisddekftsvknetkmnfglsvwacamkavklhndlfpqtaa

seqidno:126

>wp_061114504.1

mynkaeimkqawnwftdsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtva

seqidno:127

>wp_014930931.1

mynkaeimkqawnwfndsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhshlfpqtaa

seqidno:128

>wp_069002681.1

mynkaeimkqawnwftdsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskreelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtva

seqidno:129

>wp_012581436.1

mfnkaeimkqawnwftdsnvwlsdiewvsytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglrfnisddekftsvkdetkqhfglsvwacamkavklhndlfpqtaa

seqidno:130

>wp_010989940.1

mynkaeimkqawnwfndsniwlsdiewvsytdkeksfsvclkaawskakeeveeskkeskhiakseelkawnwaeiklglrfnisddekftsvkdetkmnfdlnvwacamkavklhndlfpqtaa

seqidno:131

>wp_015967156.1

mynkaeimkqawnwftdsnvwlsdiewvsytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglrfnisddekftsvkdetkqhfglsvwacamkavklhndlfpqtaa

seqidno:132

>wp_031645844.1

mynkaeimkqawncfndsnvwlsdiewvsytdkektfsvclkaawskakeeiekskkeskhiakseelkawnwaerklglrfnisddekftsvkdetkqhfglsvwacamkavklhndlfpqtaa

seqidno:133

>wp_031691597.1

mynkaeimkqawncfndsnvwlsdiewvsytdkektfsvclkaawskakeeieeskkeskhiakseelkawnwaerklglrfnisddekftsvkdetkqhfglsvwacamkavklhndlfpqtaa

seqidno:134

>wp_023553812.1

mynkseimqqawnwfrdssvwlsdiewvsytdkektfsvclkaawskakeeveeskkeskhiakseelkawnwaesklglrfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtaa

seqidno:135

>wp_069029656.1

mynkaeimkqawnwftdsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskreelkawnlaerklglhfnisddekftsvkdetkinfglsvwacamkavklhndlfpqtva

seqidno:136

>wp_069001927.1

mfnkaeimkqawnwftdsnvwlsdiewasytdkektfsvclkaawskakeevkevekeikhiskseelkawnwaerklglhfnisddekftsvkdetkinfglsvwacamkavklhn

seqidno:137

>wp_064659125.1

mkkvtydksgimkeawnlfnndditladfehlgwmewksektfalclkeawgrekevvervnqkfanaetseeakawdwackklgvafemdaytkmtnvedmekeawpgtsvwslamravklhmevaa

seqidno:138

>wp_012678885.1

mrynkseimknawamfnscnwgaenfkfvsveektfaaclkeawaeekeyveekikesanapkseeakawdwacrklnanklqnveatdkvawvsemakemwssniwaqaikavklhiklfaa

seqidno:139

>wp_037595340.1

mkynkseimknawtmfnddnfdtsyyeyataevygqktfseclkeswgrekayqeekekrlvdapkseeakawdwacrklnvnelqnidatdkvfyvegmakemwssnvwaqaikavklhielfva

seqidno:140

>wp_009730539.1

mkkvaydksgimkeawemfnrnyqicdfeyadfsgreyfeyasfadclkeawahekevvervnqkyadaetseevkawdwackklgvafemdaytkitnvegmekeawpgtsvwslamravklhmevaa

seqidno:141

>wp_071127625.1

makynkseimtqawtlfnsdnfdtcdyeyttalvygqknfsdclkeawgrekaivermaeqeanaplseeakawdwacrklgvtaevtavekvryvddmakemwsanvwkqaikavqlyatva

seqidno:142

>agm98706.1

makynkseimtqawtlfnsdnfdtcdyeyatalvygqktfsdclkeawgrekaivermaekeanaplseeakawdwacrklgvtaevtavekvryvddmakemwstnvwkqaikavqlyatva

seqidno:143

>wp_012767357.1

makynkseimknawamfnsyewdvenfkfvsaenktfsnclkeawaeekeyverkaketaeapkseeakawdwacrklnvndlqnidatdkvfyvvdmqkemwtsnvwaqaikavelyvklgla

seqidno:144

>wp_023611744.1(链球菌)

mtkynkseimknawamfnsyewdvenfkfvsaenktfsnclkeawaeekeyverkaketaeaprseeakawdwacrklnvndlqnidatdkvfyvvdmqkemwtsnvwaqaikavelyvklgla

seqidno:145

>wp_066028552.1

mtkynkseimknawamfnsyewdvenfkfvsaenktfsnclkeawaeekeyverkaketaeapkseeakawdwacrklnvndlqnidatdkvfyvvdmqkemwtsniwaqaikavelyvklgla

seqidno:146

>wp_003055844.1

mtkynkseimknawamfnsyewdvenfkfvsaenktfsnclkeawaeekeyverkakeaaeaskseeakawdwacrklnvndlqnidatnkvfyvvdmqkemwtsnvwaqaikavelyvklgla

acriia4蛋白序列

seqidno:147

>wp_003723290.1

mnindlireiknkdytvklsgtdsnsitqliirvnndgneyvisesenesivekfisafkngwnqeyedeeefyndmqtitlkseln

seqidno:148

>wp_046376634.1

mninelireiknkdytaklsgtdsnsitqliirvnndgneyvisesenesivekfisafkngwnqeyedeeefyndmqtitlkseln

seqidno:149

>wp_069001216.1

mninelirevknkdytaklsgtdsnsitqliirvnndgneyvisesenesivekfisafkngwnqeyedeeefyndmqtitlkseln

seqidno:150

>kli10194.1

mninelireiknkdytaklsgtdsnsiaqliirvnndgneyvisesenesivekfisafkngwnqeyedeeefyndmqtitlkseln

seqidno:151

>wp_031667946.1

mninelireiknkdytaklsgtdsnsitqliihvnndgneyvisesenesivekfisafkngwnqeyedeeefyndmqtitlkseln

seqidno:152

>wp_070295973.1

mnindlireiknkdytvklsgtdsnsitqliinvnndgneygisesnfesivekfvsnfengwdgayedeeefyndmqaiilkseln

seqidno:153

>wp_061107115.1

mnindlireiknkdytvklsgtdsnsitqliinvnndgneygisesnfesivekfvsnfengwdgayedeeefyndmqaiilksesn

seqidno:154

>wp_060954847.1

mniselireiknkdytvrlegtddnsitkliidvdndgneyvisesknesiaekfastfkngwnkeyedeeefyndmqsiilkseln

seqidno:155

>wp_012582157.1

mniselireiknkdyavrlegtddnsitkliidvdndgneyvisesknesiaekfastfkngwnkeyedeeefyndmqsiilkseln

seqidno:156

>car82813.1

magylkryaedrgwtlyrlakeshlsdstlrtadlttlnklsvinikkiseavgetpgevlddlikfeervekmniselireiknkdyavrlegtddnsitkliidvdndgneyvisesknesiaekfastfkngwnkeyedeeefyndmqsiilkseln

seqidno:157

>wp_003740262.1

mnlkelvreiknkdytaklsgtdsnsitqliihvnndgneygisesnfesivekfvstfengwdgayedeeefyndmqdivnrhfk

seqidno:158

>wp_061385557.1

mnlkelvreiknkdytaklsgtdsnsitqliihvnndgneygisesnfesivekfvsnfengwdgayedeeefyndmqdivnrhfk

seqidno:159

>cul91420.1

mkinelvreiksrdytvrlngtdsnsitkliidvnndgneyviserqdtsivesfadsfidgwtgtyedeedfyndmqeiaqdiiletlkeafennnyntdevdtdlfdgyqiklameydnigelatsvnktkhftaymdastdfmiieky

seqidno:160

>yp_008239985.1

msiiaikkeihakgykvtgthqgyiaqinfdgtgneyplpatwdefietfkdgwngtyedeqaffndmqevalkeildeltgalfcqdittydftiddvkkkvitldkptfeedaedliiefdstcfwdatvendkikitvrnksry

seqidno:161

>aii27415.1

msiiaikkeihakgykvtgthqgyiaqinfdgtgneyplpatwdefietfkdgwngtyedeqaffndmqeialdeildelidvlynldittynftidds

seqidno:162

>yp_001468568.1

msiiaikkeihakgykvtgthqgyiaqinfdgtgneyplpatwdefietfkdgwngtyedeqaffndmqeialeeildeltgalfcqdittydftiddvkkkvitldkptfeedaedliiefdstcfwdatvendkikitvrnksry

seqidno:163

>yp_009043548.1

msiiainkeirakgykvtgthqgyiaqinfegtgneyplpatwdefietfkdgwngtyedeqaffndmqeialdeildelidvlynldittynftiddvkkkvitlnkpideeetedlvqefnvtcfwdatveddkvkvtirnknrais

seqidno:164

>aid17477.1

msttainkeihakgykvtgthqgyiaqinfdgtgneyplpatwekfietfkdgwdgtyedeqaffndmqeialdeildelidvlynldittynftiddvkkkvitlnkpideeetedlvqefnvtcfwnaiveddkvkitvrnksk

seqidno:165

>yp_009043010.1

msttainkeihakgykvtgthqgyiaqinfdgtgneyplpatweefietfkdgwdgtyedeqaffndmqeialdeildelidvlynldittynftiddvkkkvitlnkpideeetedlvqefnvtcfwnaiveddkvkitvrnksk

seqidno:166

>aid17274.1

msttainkeihakgykvtgthqgyvaqinfdgtgneyplpatweefietfkdgwdgtykdeqaffndmqeialdeildelidtlynldittydftiddikkkvitldkptdreetedlvqefnvtcfwnaiveddkvkvtvrnksk

seqidno:167

>aay53411.1

mtgthqgyiaqinfdgtgneyplpatwdefietfkdgwngtyedeqaffndmqevalkeildelidvlynldittynftiddvkkkvitlnkptdeedaedlviefdstcfxdatvendkikvtvrnksk

seqidno:168

>yp_009044467.1

msttainkeihakgykvtgthqgymaqinfdgtgnefplpatweefietfkdgwdgtyedeqaffndmqevaleelldeltdvfynlditaydftvddvkkkvitldkptdreetedlvqefkatcfwnavveddkvkvtirnknrais

其它蛋白质序列

seqidno:169

acriia3b.3,olf47316.1

mqfvvtnkselfkfawkifkankdiafseclqnawfqykrylnreaikaaqqrklakfiadteneevkawnwaekklgvalnltdaekernvrnmykemwnanvwataikavklhmeig

seqidno:170

acriia4b.2,yp008240385.1

mnelrslemsinakkydtrlesgnrvlnigfgdgedypvcsssryslkesfiecfkdgwsgtyrdekelmedmqeiaqelileeltdifeyyefntdeidtdlfkgftfdvdsdledsmalmkainatkyfearssswyasfevsyig

序列表

<110>加利福尼亚大学董事会(theregentsoftheuniversityofcalifornia)

j·邦迪-蒂诺米(bondy-denomy,joseph)

b·劳奇(rauch,benjamin)

<120>crispr-cas9抑制剂

<130>1064235

<150>us62/422,850

<151>2016-11-16

<160>209

<170>patentinversion3.5

<210>1

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>1

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnilelyslys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>2

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>2

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluserthrasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnilelyslys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>3

<211>148

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>3

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluserasnilegluvalleuprophe

100105110

thrpheasnargphegluasnglugluhisvalasnileglylysasp

115120125

valcyslysalaleugluasnalailethrvalleulysglulyslys

130135140

asngluleuleu

145

<210>4

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>4

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>5

<211>148

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>5

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluserasnilegluvalleuprophe

100105110

thrpheasnargphegluasnglulyshisvalasnilelyslysasp

115120125

valcyslysalaleugluasnalailethrvalleulysglulyslys

130135140

asngluleuleu

145

<210>6

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>6

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasnlystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>7

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>7

metalailelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglulyshisvalasnilelyslys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>8

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>8

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>9

<211>147

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>9

metlysleuleuaspglupheleulyslyshisaspleuthrargtyr

151015

glnleuserlysleuthrglyileserglnasnthrleulysaspgln

202530

asnglulysproleuasnlystyrthrvalserileleuargserleu

354045

serleuileserglyleuservalseraspvalleuphegluleuglu

505560

aspileglulysasnseraspaspleualaglyphelyshisleuleu

65707580

asplystyrlysleuserpheproalaglngluphegluleutyrcys

859095

leuilelysgluphegluseralaasnilegluvalleuprophethr

100105110

pheasnargphegluasnglulyshisvalasnilelyslysaspval

115120125

cyslysalaleugluasnalailethrvalleulysglulyslysasn

130135140

gluleuleu

145

<210>10

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>10

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasnlystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>11

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>11

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>12

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>12

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysserleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuglylystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>13

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>13

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleuthr

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>14

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>14

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysserleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuglylystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleuthr

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>15

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>15

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysserleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuglylystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleuthr

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysalalys

130135140

lysglugluleuleu

145

<210>16

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>16

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysserleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuglylystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleuthr

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysthrleuasnasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>17

<211>144

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>17

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleuthr

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysglulys

130135140

<210>18

<211>152

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>18

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysserleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuglylystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleuthr

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysglulys

130135140

lyslysasncystyrlysasntyr

145150

<210>19

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>19

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysserleuasnlystyrthrvalserileleuarg

354045

alaleualaleuilethrglymetproileseraspvalleupheglu

505560

leugluaspleuglulysasnalaaspaspleualaglyphelyshis

65707580

leuleuaspthrtyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluphemet

145

<210>20

<211>133

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>20

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysala

130

<210>21

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>21

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysthrleuasnlystyrthrvalserileleuarg

354045

alaleualaleuilethrglymetproileseraspvalleupheglu

505560

leugluaspleuglulysasnalaaspaspleualaglyphelyshis

65707580

leuleuaspthrtyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspvalglnlysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluphemet

145

<210>22

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>22

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysthrleuasnlystyrthrvalserileleuarg

354045

alaleualaleuilethrglymetproileseraspvalleupheglu

505560

leugluaspleuglulysasnalaaspaspleualaglyphelysgln

65707580

leuleuaspthrhislysleuserpheproalahisgluphegluleu

859095

tyrcysleuilelysgluphegluservalasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluphemet

145

<210>23

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>23

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysthrleuasnlystyrthrvalserileleuarg

354045

alaleualaleuilethrglymetproileseraspvalleupheglu

505560

leugluaspleuglulysasnalaaspaspleualaglyphelysgln

65707580

leuleuaspthrhislysleuserpheproalahisgluphegluleu

859095

tyrcysleuilelysgluphegluservalasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasnglupheile

145

<210>24

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>24

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysthrleuasnlystyrthrvalserileleuarg

354045

alaleualaleuilethrglymetproileseraspvalleupheglu

505560

leugluaspleuglulysasnalaaspaspleualaglyphelysgln

65707580

leuleuaspthrhislysleuserpheproalahisgluphegluleu

859095

tyrcysleuilelysgluphegluservalasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspvalglnlysalaleugluasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>25

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>25

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysproleuasnlystyrthrvalserileleuarg

354045

serleuserleuileserglyleuservalseraspvalleupheglu

505560

leugluaspileglulysasnseraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasn

115120125

<210>26

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>26

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnilelyslys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>27

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>27

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnilelyslys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>28

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>28

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>29

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>29

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasnlystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheaspseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>30

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>30

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>31

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>31

metasnilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalpheileglu

505560

leualagluleuglulysserhisaspaspleualaglyphelyshis

65707580

leuleuasplyshislysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>32

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>32

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalleuileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>33

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>33

metthrilelysleuleuaspglupheleulyslyshisserleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalleuileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>34

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>34

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalleuileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>35

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>35

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalleuileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>36

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>36

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>37

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>37

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalpheileglu

505560

leualagluleuglulysasntyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>38

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>38

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheasnglu

505560

leuglugluleuglulysasntyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheseralaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>39

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>39

metthrilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>40

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>40

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>41

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>41

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuleu

145

<210>42

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>42

metthrilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisthraspileglulys

115120125

aspvallyslysthrleuasnasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>43

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>43

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>44

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>44

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserphemetarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>45

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>45

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalphelysglu

505560

leuglugluleuglulysasntyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheseralaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>46

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>46

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleuserglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>47

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>47

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaserilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>48

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>48

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalpheileglu

505560

leualagluleuglulysserhisaspaspleualaglyphelyshis

65707580

leuleuasplyshislysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>49

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>49

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>50

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>50

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysglupheglucysalaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspilearglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>51

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>51

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrthrvalserpheleuarg

354045

thrleusermetcysvalglymetserthrvalaspvalpheileglu

505560

leualagluleuglulysasntyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>52

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>52

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleuasnasnalailealavalleulysglulys

130135140

lysglugluleuleu

145

<210>53

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>53

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelystyr

65707580

leuleuasplyshislysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvalarglysalaleugluasnalailethrvalleulysglulys

130135140

lysasngluleuile

145

<210>54

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>54

metasnilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysthrglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplyshislysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleulysalalys

130135140

lysglugluleuleu

145

<210>55

<211>130

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>55

metserilelysleuleuaspglupheleulyslyshisaspleuthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleulys

202530

aspglnasnglulysthrleuasnlystyrthrvalserileleuarg

354045

alaleualaleuilethrglymetproileseraspvalleupheglu

505560

leugluaspleuglulysasnalaaspaspleualaglyphelysgln

65707580

leuleuaspthrhislysleuserpheproalahisgluphegluleu

859095

tyrcysleuilelysgluphegluservalasnilegluvalleupro

100105110

phethrpheasnargpheglusergluthrhisvalaspileglulys

115120125

aspval

130

<210>56

<211>134

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>56

thrargtyrglnleuserlysleuthrglyileserglnasnthrleu

151015

asnasptyrasnlyslysgluleuasnlystyrservalserpheleu

202530

argalaleusermetcysalaglyileserthrpheaspvalpheile

354045

gluleualagluleuglulyssertyraspaspleualaglyphelys

505560

hisleuleuasplystyrlysleuserpheproalaglnglupheglu

65707580

leutyrcysleuilelysgluphegluseralaasnilegluvalleu

859095

prophethrpheasnargphegluasnglugluhisvalasnilelys

100105110

lysaspvalcyslysalaleugluasnalailethrvalleulysglu

115120125

lyslysasngluleuleu

130

<210>57

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>57

metlysileasnleuleuaspglupheleulysarghisasnilethr

151015

argtyrargleuserlysleualaglyileserglnasnthrleulys

202530

asptyrasnglulysserleuasnlystyrthrvalserpheleuarg

354045

serleuserphevalthrglygluaspvalthraspvalleuileglu

505560

leualagluleuglulysglytyraspaspleualaglyphelystyr

65707580

leuleuasplystyrlysleuserpheproalaleugluphegluleu

859095

tyrcysileilelysgluphegluseralaasnilegluileserpro

100105110

phethrpheasnargphegluasngluthrhisvalaspileglulys

115120125

aspvallyslysalaleuglnasnalavalthrvalleuglugluarg

130135140

lysglugluleuleu

145

<210>58

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>58

metlysasnasnleuleuaspthrpheleulysarghisaspilethr

151015

argtyrargleuserlysleualaglyileserglnasnthrleulys

202530

asptyrasnglulysserleuasnlystyrthrvalserleuleuarg

354045

serleuserphevalthrglygluserilethraspvalleuleuglu

505560

leualagluileglulysasptyraspaspleualaglyphelystyr

65707580

leuleuasplystyrlysleuserpheproalaleugluphegluleu

859095

tyrcysileilelysgluphegluseralaasnvalgluileserpro

100105110

phethrpheasnargphegluasngluthrhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailethrvalleulysglulys

130135140

lysglugluleuleu

145

<210>59

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>59

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelystyr

65707580

leuleuasplyshislysleuserpheprothrglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleugluglulys

130135140

lysglugluleuleu

145

<210>60

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>60

metlysileasnleuleuaspalapheleulysarghisasnilethr

151015

argtyrargleuserlysleualaglyileserglnasnthrleulys

202530

asptyrasnglulysserleuasnlystyrthrvalserpheleuarg

354045

serleuserphevalthrglygluaspvalthraspvalleuileglu

505560

leualagluleuglulysglytyraspaspleualaglyphelystyr

65707580

leuleuasplystyrlysleualapheproalaleugluphegluleu

859095

tyrcysleuilelysglupheglualaalaasnilegluvalserpro

100105110

phethrpheasnargphegluasngluthrhisalaaspileglulys

115120125

aspvallyslysalaleulysasnalaileilevalleulysglulys

130135140

lysglugluleuleu

145

<210>61

<211>133

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>61

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasnileglulys

115120125

aspvalcyslysala

130

<210>62

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>62

metlysileasnleuleuaspglupheleulysarghisasnilethr

151015

argtyrargleuserlysleualaglyileserglnasnthrleulys

202530

asptyrthrglulysserleuasnlystyrthrvalserpheleuarg

354045

serleuserphealathrglygluservalthraspileleuleuglu

505560

leualagluleuglulysasptyraspaspleualaglyphelystyr

65707580

leuleuasplystyrlysleualapheproalaleugluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluileserpro

100105110

phethrpheasnargpheglusergluthrhisthraspileglulys

115120125

aspvallyslysalaleuglnasnalavalthrvalleuglugluarg

130135140

lysglugluleuleu

145

<210>63

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>63

metserilelysleuleuaspglupheleulyslyshisserlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

serleuserphealathrglygluservalthraspileleuleuglu

505560

leualagluleuglulysasptyraspaspleualaglyphelystyr

65707580

leuleuasplystyrlysleualapheproalaleugluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluileserpro

100105110

phethrpheasnargpheglusergluthrhisthraspileglulys

115120125

aspvallyslysalaleuglnasnalavalthrvalleuglugluarg

130135140

lysglugluleuleu

145

<210>64

<211>159

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>64

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelystyr

65707580

leuleuasplyshislysleuserpheprothrglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasngluthrhisalaaspileglulys

115120125

aspvallyslysalaleuasnasnalailealavalleugluglulys

130135140

lysargargthrvalilelysthrileasptyrtyrasptyrser

145150155

<210>65

<211>148

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>65

metasnasnphealapheilethrserpheasntyrglnglnproarg

151015

tyrglnleuserlysleuthrglyileserglnasnthrleuasnasp

202530

tyrasnlyslysgluleuasnlystyrservalserpheleuargala

354045

leusermetcysalaglyileserthrpheaspvalleuilegluleu

505560

alagluleuglulyssertyraspaspleualaglyphelyshisleu

65707580

leuasplystyrlysleuserpheproalaglngluphegluleutyr

859095

cysleuilelysgluphegluseralaasnilegluvalleuprophe

100105110

thrpheasnargphegluasngluthrhisvalaspileglulysasp

115120125

valarglysalaleugluasnalailethrvalleulysglulyslys

130135140

asngluleuile

145

<210>66

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>66

metlysthrasnleuleuaspthrpheleulysarghisglyilethr

151015

argtyrargleuserlysleualaglyileserglnasnthrleulys

202530

asptyrthrglulysserleuasnlystyrthrvalserpheleuarg

354045

serleuserphevalthrglygluaspvalthraspvalleuleuglu

505560

leualagluilegluasnglytyraspaspleualaglyphelystyr

65707580

leuleuasplystyrlysleuserpheproalaleugluphegluleu

859095

tyrcysileilelysgluphegluseralaasnilegluileserpro

100105110

phethrpheasnargphegluasngluthrhisalaaspileglulys

115120125

aspvallyslysalaleulysasnalavalthrvalleuglugluarg

130135140

lysglugluleuleu

145

<210>67

<211>148

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>67

metasnasnphealapheilethrserpheasntyrglnglnproarg

151015

tyrglnleuserlysleuthrglyileserglnasnthrleuasnasp

202530

tyrasnlyslysgluleuasnlystyrservalserpheleuargala

354045

leusermetcysalaglyileserthrpheaspvalleuilegluleu

505560

alagluleuglulyssertyraspaspleualaglyphelyshisleu

65707580

leuasplystyrlysleuserpheproalaglngluphegluleutyr

859095

cysleuilelysglupheglucysalaasnilegluvalleuprophe

100105110

thrpheasnargphegluasngluthrhisvalaspileglulysasp

115120125

valarglysalaleugluasnalailethrvalleulysglulyslys

130135140

asngluleuile

145

<210>68

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>68

metserilelysleuleuaspglupheleulyslyshisasnlysthr

151015

argtyrglnleuserlysleuthrglyileserglnasnthrleuasn

202530

asptyrasnlyslysgluleuasnlystyrservalserpheleuarg

354045

alaleusermetcysalaglyileserthrpheaspvalpheileglu

505560

leualagluleuglulyssertyraspaspleualaglyphelyshis

65707580

leuleuasplystyrlysleuserpheproalaglngluphegluleu

859095

tyrcysleuilelysgluphegluseralaasnilegluvalleupro

100105110

phethrpheasnargphegluasnglugluhisvalasn

115120125

<210>69

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>69

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleulys

115120

<210>70

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>70

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleuasn

115120

<210>71

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>71

metthrilethrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleulys

115120

<210>72

<211>124

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>72

lysmetthrleuthrargalaglnlyslystyralaglualamethis

151015

glupheileasnmetvalaspasppheglugluserthrproaspphe

202530

alalysgluvalleuhisaspserasptyrvalvalilethrlysasn

354045

glulystyralavalalaleucysserleuserthraspglucysglu

505560

tyraspthrasnleutyrleuaspglulysleuvalasptyrserthr

65707580

valaspvalasnglyvalthrtyrtyrileasnilevalgluthrasn

859095

aspileaspaspleugluilealathraspgluaspglumetlysser

100105110

glyasnglngluileileleulyssergluleuasn

115120

<210>73

<211>122

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>73

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleu

115120

<210>74

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>74

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlysserasp

100105110

asnglngluileileleulyssergluleulys

115120

<210>75

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>75

metthrilethrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglycysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleulys

115120

<210>76

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>76

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrlysasp

859095

ileaspaspleugluilealathraspgluaspglumetlysserasp

100105110

asnglngluileileleulyssergluleulys

115120

<210>77

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>77

metthrilethrthralaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleulys

115120

<210>78

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>78

metthrilethrthralaglnarglystyrasnglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlyssergly

100105110

asnglngluileileleulyssergluleulys

115120

<210>79

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>79

metthrilethrthralaglnarglystyrasnglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspcysasptyrvalvalvalthrlysasnglu

354045

lystyralavalalaleucysthrleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

asnvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlysserasp

100105110

asnglngluileileleulyssergluleulys

115120

<210>80

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>80

metthrilethrthralaglnarglystyrasnglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspcysasptyrvalvalvalthrlysasnglu

354045

lystyralavalalaleucysthrleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

asnvalasnglyvalthrtyrtyrileasnilevalgluthrasnasp

859095

ileaspaspleugluilealathraspgluaspglumetlysserasp

100105110

asnglnlysileileleulyssergluleulys

115120

<210>81

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>81

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproasppheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalgluthrlysasp

859095

ileaspaspleugluilealathraspgluasplysglulyshisasp

100105110

lysglngluvalileilelyssergluleuasn

115120

<210>82

<211>110

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>82

methisglupheileasnmetvalaspasppheglugluserthrpro

151015

aspphealalysgluvalleuhisaspserasptyrvalvalilethr

202530

lysasnglulystyralavalalaleucysserleuserthraspglu

354045

cysglutyraspthrasnleutyrleuaspglulysleuvalasptyr

505560

serthrvalaspvalasnglyvalthrtyrtyrileasnilevalglu

65707580

thrasnaspileaspaspleugluilealathraspgluaspglumet

859095

lysserglyasnglngluileileleulyssergluleulys

100105110

<210>83

<211>122

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>83

metthrleuthrargalaglnlyslystyralaglualamethisglu

151015

pheileasnmetvalaspasppheglugluserlysproasnpheala

202530

lysgluvalleuhisaspserasptyrvalvalilethrlysasnglu

354045

lystyralavalalaleucysserleuserthraspglucysglutyr

505560

aspthrasnleutyrleuaspglulysleuvalasptyrserthrval

65707580

aspvalasnglyvalthrtyrtyrileasnilevalvalthrasnglu

859095

aspaspphelysleualathrasplysasplysglulyshisasplys

100105110

glngluvalilevallyssergluleuasn

115120

<210>84

<211>122

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>84

metthrleuthrthralaglnarglystyrasnglualamethisglu

151015

pheileasnmetvalaspasppheglugluserthrproglupheser

202530

lysgluvalleuasnaspserasptyrvalvalilethrlysasnglu

354045

lystyralaglyalaleucyshisvalserthraspglucysgluasp

505560

glyserasnleutyrileaspglulysleuileasptyrserthrleu

65707580

asnvalglyglyvalthrtyrtyrileasnilevalgluargcysglu

859095

aspaspleugluilealathraspgluasplysmetlysseraspasn

100105110

glngluileileleulysasngluleuasn

115120

<210>85

<211>103

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>85

metvalaspasppheglugluserthrproglupheserlysgluval

151015

leuasnaspserasptyrvalvalilethrlysasnglulystyrala

202530

glyalaleucyshisvalserthraspglucysgluaspglyserasn

354045

leutyrileaspglulysleuileasptyrserthrleuasnvalgly

505560

glyvalthrtyrtyrileasnilevalgluargcysgluaspaspleu

65707580

gluilealathraspgluasplysmetlysseraspasnglngluile

859095

ileleulysasngluleuasn

100

<210>86

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>86

metthrleuthrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlyslysleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysmetleuaspaspasplysleutyrleu

505560

aspgluthrleuvalglnserthrcysleuaspvalgluglygluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalilevallys

100105110

sergluleuasn

115

<210>87

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>87

metthrleuthrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysmetleuaspaspasplysleutyrleu

505560

aspgluthrleuvalglnserthrcysleuaspvalgluglygluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalilevallys

100105110

sergluleuasn

115

<210>88

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>88

metthrleuthrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysmetleuaspaspasplysleutyrleu

505560

aspgluthrleuvalglnserthrcysleuaspvalgluglygluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thrasplysasplysglulyshisasplysglngluvalilevallys

100105110

sergluleuasn

115

<210>89

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>89

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualaileileserlysgluleugluglnthrproalapheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysmetleuaspaspasplysleutyrleu

505560

aspgluthrleuvalglnserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>90

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>90

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysmetleuaspaspasplysleutyrile

505560

aspgluthrleuvalglnserthrcysleuaspvalgluglygluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thrasplysasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>91

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>91

metthrleuthrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualaileileserlysgluleugluglnthrargalapheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalaspleucysmetleuaspaspasplysleutyrleu

505560

aspgluthrleuvalglnserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisaspargglngluvalileilelys

100105110

sergluleuasn

115

<210>92

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>92

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleuvalvalthrlysasnglu

354045

alatyralaalaalaleucysmetleuaspaspasplysleutyrleu

505560

aspgluthrleuvalglnserthrcysleuaspvalgluglygluile

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thrasplysasplysglulyshisasplysglngluvalilevallys

100105110

sergluleuasn

115

<210>93

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>93

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysglyleugluglnthrproglupheser

202530

glnaspleuleuasnasppheasptyrleuvalilethrlysasnglu

354045

alatyralaalaalaleucysmetleuaspaspglulysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspaspthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

arggluleuasn

115

<210>94

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>94

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualaileileserlysgluleugluglnthrproalapheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>95

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>95

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualaileileserlysgluleugluglnthrproalapheser

202530

glnasnleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>96

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>96

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproalapheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalilevallys

100105110

sergluleuasn

115

<210>97

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>97

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualaileileserlysgluleugluglnthrproalapheser

202530

glnaspleuleuasnaspserasptyrleuvalilethrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuasnilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

serglupheasn

115

<210>98

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>98

metthrleuthrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleualavalthrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

serglyleuasn

115

<210>99

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>99

metthrvalthrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleualavalthrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>100

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>100

metthrleuthrthrvalglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspleuleuasnaspserasptyrleualavalthrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargpheaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>101

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>101

metthrleuthrthrvalglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproglupheser

202530

glnaspserleuasnaspserasptyrleualavalthrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargpheaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

sergluleuasn

115

<210>102

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>102

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualaileileserlysgluleugluglnthrproalapheser

202530

glnaspleuleuasnaspserasptyrleualavalthrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

serglyleuasn

115

<210>103

<211>116

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>103

metthrilethrthralaglnlysargtyrtyraspalametasnglu

151015

pheglualailethrserlysgluleugluglnthrproalapheser

202530

glnaspleuleuasnaspserasptyrleualavalthrlysasnglu

354045

alatyralavalalaleucysleuleuaspaspasplysleutyrleu

505560

aspgluthrleuvalhisserthrargleuaspilegluaspgluthr

65707580

tyrtyrileasnphevalvalthrasngluaspaspphelysleuala

859095

thraspgluasplysglulyshisasplysglngluvalileilelys

100105110

serglyleuasn

115

<210>104

<211>130

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>104

metalailethrleuserglnarglysphetyrglualaileasnglu

151015

phegluglumetthrgluasngluvalvalthrserproargilepro

202530

glnasptyrleuasnaspglyasptyrvalvalilethrlysserglu

354045

asntyralaleuasnleucysthrthrasnleugluglyphegluasp

505560

arghispheleuaspglulysleuiletyrserthrphevalgluthr

65707580

tyrserglygluthrtyrtyriletyrilethrglnthralagluphe

859095

aspgluaspaspalavalglupheleualathrglngluglniletyr

100105110

glutyrhislysglnglugluglnlysthrvalileleulysmetglu

115120125

leuser

130

<210>105

<211>130

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>105

metalaglnthrglualaglnlysilephetyrglualaileasnglu

151015

phegluglumetthrasnglugluvalvalthrserproargilepro

202530

glnasptyrleuasnaspglyasptyrvalvalilethrlysserglu

354045

asntyralaleuasnleucysthrthraspleugluglyphegluasp

505560

argtyrpheleuaspglulysleuiletyrserthrservalgluthr

65707580

tyrthraspgluthrtyrtyriletyrilethrglnthrthrgluphe

859095

glugluaspasnalavalglupheleualathrglngluglniletyr

100105110

glutyrhislysglnglugluglnlysthrvalileleulysmetglu

115120125

leuser

130

<210>106

<211>130

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>106

metthrthralaarglyslysphetyrglnalaileserglupheglu

151015

alametthrglylysaspvalgluargthrproglnilealaaspglu

202530

valleuasnaspalaglutyrilealaphethrlysthrglulystyr

354045

alaleutyrleucysthrserasnvalgluglyleugluaspargtyr

505560

pheleuaspgluglucysleuaspserthrpheleugluthrgluasp

65707580

asngluthrtyrtyrilehispheleuglngluthrglupheserglu

859095

aspaspasngluaspgluleuproleualathrglugluglnileglu

100105110

alatyrasplysglnglugluleulysalavalileleulyslysglu

115120125

leuasn

130

<210>107

<211>129

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>107

metargthrthralaglngluargleuaspasnalaileasngluphe

151015

glugluilethrasnglugluvalvalthrserproargileprogln

202530

asptyrleuasnaspglyasptyrvalvalilethrlyssergluasn

354045

tyralaleuasnleucysthrthrasnleugluglyphegluasparg

505560

hispheleuaspglulysleuiletyrserthrphevalgluthrtyr

65707580

serglygluthrtyrtyriletyrilethrglnthralaglupheasp

859095

gluaspaspalavalglupheleualathrglngluglniletyrglu

100105110

tyrhislysglnglugluglnlysthrvalileleulysmetgluleu

115120125

ser

<210>108

<211>129

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>108

metargthrthralaglngluargleuaspasnalaileasngluphe

151015

glugluilethrasnglugluvalvalthrserproargileprogln

202530

asptyrleuasnaspglyasptyrvalvalilethrlyssergluasn

354045

tyralaleuasnleucysthrthrasnleugluglyphegluasparg

505560

hispheleuaspglulysleuiletyrserthrphevalgluthrtyr

65707580

alaglygluthrtyrtyriletyrilethrglnthralaglupheasp

859095

gluaspaspalavalglupheleualathrglngluglniletyrglu

100105110

tyrhislysglnglugluglnlysthrvalileleulysmetgluleu

115120125

ser

<210>109

<211>129

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>109

metargthrthralaglngluargleuaspasnalaileasngluphe

151015

glugluilethrasnglugluvalvalthrserproleuileprogln

202530

asptyrleuasnaspglyasptyrvalvalilethrlyssergluasn

354045

tyralaleuasnleucysthrthrasnleugluglyphegluasparg

505560

hispheleuaspglulysleuiletyrserthrphevalgluthrtyr

65707580

serglygluthrtyrtyriletyrilethrglnthralaglupheasp

859095

gluaspaspalavalglupheleualathrglngluglniletyrglu

100105110

tyrhislysglnglugluglnlysthrvalileleulysmetgluleu

115120125

ser

<210>110

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>110

metpheasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>111

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>111

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>112

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>112

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysglyalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>113

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>113

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuserleutrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>114

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>114

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasnvaltrpleuseraspileglutrpilesertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>115

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>115

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlystyrilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>116

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>116

metpheasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>117

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>117

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluphelyslysgluserlystyrilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>118

<211>118

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>118

metlysglnalatrpasntrppheasnaspserasniletrpleuser

151015

aspileglutrpvalsertyrthrasplysglulysserpheserval

202530

cysleulysalaalatrpserlysalalysglugluvalglugluser

354045

lyslysgluserlystyrilealalysserglugluleulysalatrp

505560

asntrpalagluarglysleuglyleuargpheasnileseraspasp

65707580

glulysphethrservallysaspgluthrlysileasnpheglyleu

859095

servaltrpalacysalametlysalavallysleuhisasnaspleu

100105110

pheproglnthralaala

115

<210>119

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>119

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

asnserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlysglugluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>120

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>120

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

asnserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleuargalaalatrpserlysala

354045

lysglugluvalglugluserlysglugluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>121

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>121

metpheasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>122

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>122

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>123

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>123

metpheasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>124

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>124

mettyrasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>125

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>125

metpheasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluphelysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysasnglu

859095

thrlysmetasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>126

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>126

mettyrasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthrvalala

115120125

<210>127

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>127

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisserhisleupheproglnthralaala

115120125

<210>128

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>128

mettyrasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

argglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthrvalala

115120125

<210>129

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>129

metpheasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysglnhispheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>130

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>130

mettyrasnlysalagluilemetlysglnalatrpasntrppheasn

151015

aspserasniletrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysserpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluilelysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysmetasnpheaspleuasnvaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>131

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>131

mettyrasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysglnhispheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>132

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>132

mettyrasnlysalagluilemetlysglnalatrpasncyspheasn

151015

aspserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluileglulysserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysglnhispheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>133

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>133

mettyrasnlysalagluilemetlysglnalatrpasncyspheasn

151015

aspserasnvaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluileglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysglnhispheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>134

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>134

mettyrasnlyssergluilemetglnglnalatrpasntrpphearg

151015

aspserservaltrpleuseraspileglutrpvalsertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvalglugluserlyslysgluserlyshisilealalys

505560

serglugluleulysalatrpasntrpalagluserlysleuglyleu

65707580

argpheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthralaala

115120125

<210>135

<211>125

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>135

mettyrasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

argglugluleulysalatrpasnleualagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasnaspleupheproglnthrvalala

115120125

<210>136

<211>117

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>136

metpheasnlysalagluilemetlysglnalatrpasntrpphethr

151015

aspserasnvaltrpleuseraspileglutrpalasertyrthrasp

202530

lysglulysthrpheservalcysleulysalaalatrpserlysala

354045

lysglugluvallysgluvalglulysgluilelyshisileserlys

505560

serglugluleulysalatrpasntrpalagluarglysleuglyleu

65707580

hispheasnileseraspaspglulysphethrservallysaspglu

859095

thrlysileasnpheglyleuservaltrpalacysalametlysala

100105110

vallysleuhisasn

115

<210>137

<211>128

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>137

metlyslysvalthrtyrasplysserglyilemetlysglualatrp

151015

asnleupheasnasnaspaspilethrleualaaspphegluhisleu

202530

glytrpmetglutrplysserglulysthrphealaleucysleulys

354045

glualatrpglyargglulysgluvalvalgluargvalasnglnlys

505560

phealaasnalagluthrsergluglualalysalatrpasptrpala

65707580

cyslyslysleuglyvalalapheglumetaspalatyrthrlysmet

859095

thrasnvalgluaspmetglulysglualatrpproglythrserval

100105110

trpserleualametargalavallysleuhismetgluvalalaala

115120125

<210>138

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>138

metargtyrasnlyssergluilemetlysasnalatrpalametphe

151015

asnsercysasntrpglyalagluasnphelysphevalservalglu

202530

glulysthrphealaalacysleulysglualatrpalagluglulys

354045

glutyrvalgluglulysilelysgluseralaasnalaprolysser

505560

gluglualalysalatrpasptrpalacysarglysleuasnalaasn

65707580

lysleuglnasnvalglualathrasplysvalalatrpvalserglu

859095

metalalysglumettrpserserasniletrpalaglnalailelys

100105110

alavallysleuhisilelysleuphealaala

115120

<210>139

<211>126

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>139

metlystyrasnlyssergluilemetlysasnalatrpthrmetphe

151015

asnaspaspasnpheaspthrsertyrtyrglutyralathralaglu

202530

valtyrglyglnlysthrpheserglucysleulysglusertrpgly

354045

argglulysalatyrglngluglulysglulysargleuvalaspala

505560

prolyssergluglualalysalatrpasptrpalacysarglysleu

65707580

asnvalasngluleuglnasnileaspalathrasplysvalphetyr

859095

valgluglymetalalysglumettrpserserasnvaltrpalagln

100105110

alailelysalavallysleuhisilegluleuphevalala

115120125

<210>140

<211>129

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>140

metlyslysvalalatyrasplysserglyilemetlysglualatrp

151015

glumetpheasnargasntyrglnilecysasppheglutyralaasp

202530

pheserglyargglutyrpheglutyralaserphealaaspcysleu

354045

lysglualatrpalahisglulysgluvalvalgluargvalasngln

505560

lystyralaaspalagluthrserglugluvallysalatrpasptrp

65707580

alacyslyslysleuglyvalalapheglumetaspalatyrthrlys

859095

ilethrasnvalgluglymetglulysglualatrpproglythrser

100105110

valtrpserleualametargalavallysleuhismetgluvalala

115120125

ala

<210>141

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>141

metalalystyrasnlyssergluilemetthrglnalatrpthrleu

151015

pheasnseraspasnpheaspthrcysasptyrglutyrthrthrala

202530

leuvaltyrglyglnlysasnpheseraspcysleulysglualatrp

354045

glyargglulysalailevalgluargmetalagluglnglualaasn

505560

alaproleusergluglualalysalatrpasptrpalacysarglys

65707580

leuglyvalthralagluvalthralavalglulysvalargtyrval

859095

aspaspmetalalysglumettrpseralaasnvaltrplysglnala

100105110

ilelysalavalglnleutyralathrvalala

115120

<210>142

<211>123

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>142

metalalystyrasnlyssergluilemetthrglnalatrpthrleu

151015

pheasnseraspasnpheaspthrcysasptyrglutyralathrala

202530

leuvaltyrglyglnlysthrpheseraspcysleulysglualatrp

354045

glyargglulysalailevalgluargmetalaglulysglualaasn

505560

alaproleusergluglualalysalatrpasptrpalacysarglys

65707580

leuglyvalthralagluvalthralavalglulysvalargtyrval

859095

aspaspmetalalysglumettrpserthrasnvaltrplysglnala

100105110

ilelysalavalglnleutyralathrvalala

115120

<210>143

<211>124

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>143

metalalystyrasnlyssergluilemetlysasnalatrpalamet

151015

pheasnsertyrglutrpaspvalgluasnphelysphevalserala

202530

gluasnlysthrpheserasncysleulysglualatrpalagluglu

354045

lysglutyrvalgluarglysalalysgluthralaglualaprolys

505560

sergluglualalysalatrpasptrpalacysarglysleuasnval

65707580

asnaspleuglnasnileaspalathrasplysvalphetyrvalval

859095

aspmetglnlysglumettrpthrserasnvaltrpalaglnalaile

100105110

lysalavalgluleutyrvallysleuglyleuala

115120

<210>144

<211>124

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>144

metthrlystyrasnlyssergluilemetlysasnalatrpalamet

151015

pheasnsertyrglutrpaspvalgluasnphelysphevalserala

202530

gluasnlysthrpheserasncysleulysglualatrpalagluglu

354045

lysglutyrvalgluarglysalalysgluthralaglualaproarg

505560

sergluglualalysalatrpasptrpalacysarglysleuasnval

65707580

asnaspleuglnasnileaspalathrasplysvalphetyrvalval

859095

aspmetglnlysglumettrpthrserasnvaltrpalaglnalaile

100105110

lysalavalgluleutyrvallysleuglyleuala

115120

<210>145

<211>124

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>145

metthrlystyrasnlyssergluilemetlysasnalatrpalamet

151015

pheasnsertyrglutrpaspvalgluasnphelysphevalserala

202530

gluasnlysthrpheserasncysleulysglualatrpalagluglu

354045

lysglutyrvalgluarglysalalysgluthralaglualaprolys

505560

sergluglualalysalatrpasptrpalacysarglysleuasnval

65707580

asnaspleuglnasnileaspalathrasplysvalphetyrvalval

859095

aspmetglnlysglumettrpthrserasniletrpalaglnalaile

100105110

lysalavalgluleutyrvallysleuglyleuala

115120

<210>146

<211>124

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>146

metthrlystyrasnlyssergluilemetlysasnalatrpalamet

151015

pheasnsertyrglutrpaspvalgluasnphelysphevalserala

202530

gluasnlysthrpheserasncysleulysglualatrpalagluglu

354045

lysglutyrvalgluarglysalalysglualaalaglualaserlys

505560

sergluglualalysalatrpasptrpalacysarglysleuasnval

65707580

asnaspleuglnasnileaspalathrasnlysvalphetyrvalval

859095

aspmetglnlysglumettrpthrserasnvaltrpalaglnalaile

100105110

lysalavalgluleutyrvallysleuglyleuala

115120

<210>147

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>147

metasnileasnaspleuilearggluilelysasnlysasptyrthr

151015

vallysleuserglythraspserasnserilethrglnleuileile

202530

argvalasnasnaspglyasnglutyrvalileserglusergluasn

354045

gluserilevalglulyspheileseralaphelysasnglytrpasn

505560

glnglutyrgluaspgluglugluphetyrasnaspmetglnthrile

65707580

thrleulyssergluleuasn

85

<210>148

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>148

metasnileasngluleuilearggluilelysasnlysasptyrthr

151015

alalysleuserglythraspserasnserilethrglnleuileile

202530

argvalasnasnaspglyasnglutyrvalileserglusergluasn

354045

gluserilevalglulyspheileseralaphelysasnglytrpasn

505560

glnglutyrgluaspgluglugluphetyrasnaspmetglnthrile

65707580

thrleulyssergluleuasn

85

<210>149

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>149

metasnileasngluleuilearggluvallysasnlysasptyrthr

151015

alalysleuserglythraspserasnserilethrglnleuileile

202530

argvalasnasnaspglyasnglutyrvalileserglusergluasn

354045

gluserilevalglulyspheileseralaphelysasnglytrpasn

505560

glnglutyrgluaspgluglugluphetyrasnaspmetglnthrile

65707580

thrleulyssergluleuasn

85

<210>150

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>150

metasnileasngluleuilearggluilelysasnlysasptyrthr

151015

alalysleuserglythraspserasnserilealaglnleuileile

202530

argvalasnasnaspglyasnglutyrvalileserglusergluasn

354045

gluserilevalglulyspheileseralaphelysasnglytrpasn

505560

glnglutyrgluaspgluglugluphetyrasnaspmetglnthrile

65707580

thrleulyssergluleuasn

85

<210>151

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>151

metasnileasngluleuilearggluilelysasnlysasptyrthr

151015

alalysleuserglythraspserasnserilethrglnleuileile

202530

hisvalasnasnaspglyasnglutyrvalileserglusergluasn

354045

gluserilevalglulyspheileseralaphelysasnglytrpasn

505560

glnglutyrgluaspgluglugluphetyrasnaspmetglnthrile

65707580

thrleulyssergluleuasn

85

<210>152

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>152

metasnileasnaspleuilearggluilelysasnlysasptyrthr

151015

vallysleuserglythraspserasnserilethrglnleuileile

202530

asnvalasnasnaspglyasnglutyrglyilesergluserasnphe

354045

gluserilevalglulysphevalserasnphegluasnglytrpasp

505560

glyalatyrgluaspgluglugluphetyrasnaspmetglnalaile

65707580

ileleulyssergluleuasn

85

<210>153

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>153

metasnileasnaspleuilearggluilelysasnlysasptyrthr

151015

vallysleuserglythraspserasnserilethrglnleuileile

202530

asnvalasnasnaspglyasnglutyrglyilesergluserasnphe

354045

gluserilevalglulysphevalserasnphegluasnglytrpasp

505560

glyalatyrgluaspgluglugluphetyrasnaspmetglnalaile

65707580

ileleulyssergluserasn

85

<210>154

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>154

metasnilesergluleuilearggluilelysasnlysasptyrthr

151015

valargleugluglythraspaspasnserilethrlysleuileile

202530

aspvalaspasnaspglyasnglutyrvalilesergluserlysasn

354045

gluserilealaglulysphealaserthrphelysasnglytrpasn

505560

lysglutyrgluaspgluglugluphetyrasnaspmetglnserile

65707580

ileleulyssergluleuasn

85

<210>155

<211>87

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>155

metasnilesergluleuilearggluilelysasnlysasptyrala

151015

valargleugluglythraspaspasnserilethrlysleuileile

202530

aspvalaspasnaspglyasnglutyrvalilesergluserlysasn

354045

gluserilealaglulysphealaserthrphelysasnglytrpasn

505560

lysglutyrgluaspgluglugluphetyrasnaspmetglnserile

65707580

ileleulyssergluleuasn

85

<210>156

<211>160

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>156

metalaglytyrleulysargtyralagluaspargglytrpthrleu

151015

tyrargleualalysgluserhisleuseraspserthrleuargthr

202530

alaaspleuthrthrleuasnlysleuservalileasnilelyslys

354045

ileserglualavalglygluthrproglygluvalleuaspaspleu

505560

ilelyspheglugluargvalglulysmetasnilesergluleuile

65707580

arggluilelysasnlysasptyralavalargleugluglythrasp

859095

aspasnserilethrlysleuileileaspvalaspasnaspglyasn

100105110

glutyrvalilesergluserlysasngluserilealaglulysphe

115120125

alaserthrphelysasnglytrpasnlysglutyrgluaspgluglu

130135140

gluphetyrasnaspmetglnserileileleulyssergluleuasn

145150155160

<210>157

<211>86

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>157

metasnleulysgluleuvalarggluilelysasnlysasptyrthr

151015

alalysleuserglythraspserasnserilethrglnleuileile

202530

hisvalasnasnaspglyasnglutyrglyilesergluserasnphe

354045

gluserilevalglulysphevalserthrphegluasnglytrpasp

505560

glyalatyrgluaspgluglugluphetyrasnaspmetglnaspile

65707580

valasnarghisphelys

85

<210>158

<211>86

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>158

metasnleulysgluleuvalarggluilelysasnlysasptyrthr

151015

alalysleuserglythraspserasnserilethrglnleuileile

202530

hisvalasnasnaspglyasnglutyrglyilesergluserasnphe

354045

gluserilevalglulysphevalserasnphegluasnglytrpasp

505560

glyalatyrgluaspgluglugluphetyrasnaspmetglnaspile

65707580

valasnarghisphelys

85

<210>159

<211>151

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>159

metlysileasngluleuvalarggluilelysserargasptyrthr

151015

valargleuasnglythraspserasnserilethrlysleuileile

202530

aspvalasnasnaspglyasnglutyrvalilesergluargglnasp

354045

thrserilevalgluserphealaaspserpheileaspglytrpthr

505560

glythrtyrgluaspglugluaspphetyrasnaspmetglngluile

65707580

alaglnaspileileleugluthrleulysglualaphegluasnasn

859095

asntyrasnthraspgluvalaspthraspleupheaspglytyrgln

100105110

ilelysleualametglutyraspasnileglygluleualathrser

115120125

valasnlysthrlyshisphethralatyrmetaspalaserthrasp

130135140

phemetileileglulystyr

145150

<210>160

<211>147

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>160

metserileilealailelyslysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrilealaglnileasnpheaspgly

202530

thrglyasnglutyrproleuproalathrtrpaspglupheileglu

354045

thrphelysaspglytrpasnglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluvalalaleulysgluileleuaspgluleu

65707580

thrglyalaleuphecysglnaspilethrthrtyraspphethrile

859095

aspaspvallyslyslysvalilethrleuasplysprothrpheglu

100105110

gluaspalagluaspleuileileglupheaspserthrcysphetrp

115120125

aspalathrvalgluasnasplysilelysilethrvalargasnlys

130135140

serargtyr

145

<210>161

<211>99

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>161

metserileilealailelyslysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrilealaglnileasnpheaspgly

202530

thrglyasnglutyrproleuproalathrtrpaspglupheileglu

354045

thrphelysaspglytrpasnglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluilealaleuaspgluileleuaspgluleu

65707580

ileaspvalleutyrasnleuaspilethrthrtyrasnphethrile

859095

aspaspser

<210>162

<211>147

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>162

metserileilealailelyslysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrilealaglnileasnpheaspgly

202530

thrglyasnglutyrproleuproalathrtrpaspglupheileglu

354045

thrphelysaspglytrpasnglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluilealaleuglugluileleuaspgluleu

65707580

thrglyalaleuphecysglnaspilethrthrtyraspphethrile

859095

aspaspvallyslyslysvalilethrleuasplysprothrpheglu

100105110

gluaspalagluaspleuileileglupheaspserthrcysphetrp

115120125

aspalathrvalgluasnasplysilelysilethrvalargasnlys

130135140

serargtyr

145

<210>163

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>163

metserileilealaileasnlysgluileargalalysglytyrlys

151015

valthrglythrhisglnglytyrilealaglnileasnpheglugly

202530

thrglyasnglutyrproleuproalathrtrpaspglupheileglu

354045

thrphelysaspglytrpasnglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluilealaleuaspgluileleuaspgluleu

65707580

ileaspvalleutyrasnleuaspilethrthrtyrasnphethrile

859095

aspaspvallyslyslysvalilethrleuasnlysproileaspglu

100105110

glugluthrgluaspleuvalglnglupheasnvalthrcysphetrp

115120125

aspalathrvalgluaspasplysvallysvalthrileargasnlys

130135140

asnargalaileser

145

<210>164

<211>146

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>164

metserthrthralaileasnlysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrilealaglnileasnpheaspgly

202530

thrglyasnglutyrproleuproalathrtrpglulyspheileglu

354045

thrphelysaspglytrpaspglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluilealaleuaspgluileleuaspgluleu

65707580

ileaspvalleutyrasnleuaspilethrthrtyrasnphethrile

859095

aspaspvallyslyslysvalilethrleuasnlysproileaspglu

100105110

glugluthrgluaspleuvalglnglupheasnvalthrcysphetrp

115120125

asnalailevalgluaspasplysvallysilethrvalargasnlys

130135140

serlys

145

<210>165

<211>146

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>165

metserthrthralaileasnlysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrilealaglnileasnpheaspgly

202530

thrglyasnglutyrproleuproalathrtrpgluglupheileglu

354045

thrphelysaspglytrpaspglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluilealaleuaspgluileleuaspgluleu

65707580

ileaspvalleutyrasnleuaspilethrthrtyrasnphethrile

859095

aspaspvallyslyslysvalilethrleuasnlysproileaspglu

100105110

glugluthrgluaspleuvalglnglupheasnvalthrcysphetrp

115120125

asnalailevalgluaspasplysvallysilethrvalargasnlys

130135140

serlys

145

<210>166

<211>146

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>166

metserthrthralaileasnlysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrvalalaglnileasnpheaspgly

202530

thrglyasnglutyrproleuproalathrtrpgluglupheileglu

354045

thrphelysaspglytrpaspglythrtyrlysaspgluglnalaphe

505560

pheasnaspmetglngluilealaleuaspgluileleuaspgluleu

65707580

ileaspthrleutyrasnleuaspilethrthrtyraspphethrile

859095

aspaspilelyslyslysvalilethrleuasplysprothrasparg

100105110

glugluthrgluaspleuvalglnglupheasnvalthrcysphetrp

115120125

asnalailevalgluaspasplysvallysvalthrvalargasnlys

130135140

serlys

145

<210>167

<211>130

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<220>

<221>misc_feature

<222>(112)..(112)

<223>xaa是任何氨基酸

<400>167

metthrglythrhisglnglytyrilealaglnileasnpheaspgly

151015

thrglyasnglutyrproleuproalathrtrpaspglupheileglu

202530

thrphelysaspglytrpasnglythrtyrgluaspgluglnalaphe

354045

pheasnaspmetglngluvalalaleulysgluileleuaspgluleu

505560

ileaspvalleutyrasnleuaspilethrthrtyrasnphethrile

65707580

aspaspvallyslyslysvalilethrleuasnlysprothraspglu

859095

gluaspalagluaspleuvalileglupheaspserthrcysphexaa

100105110

aspalathrvalgluasnasplysilelysvalthrvalargasnlys

115120125

serlys

130

<210>168

<211>149

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>168

metserthrthralaileasnlysgluilehisalalysglytyrlys

151015

valthrglythrhisglnglytyrmetalaglnileasnpheaspgly

202530

thrglyasnglupheproleuproalathrtrpgluglupheileglu

354045

thrphelysaspglytrpaspglythrtyrgluaspgluglnalaphe

505560

pheasnaspmetglngluvalalaleuglugluleuleuaspgluleu

65707580

thraspvalphetyrasnleuaspilethralatyraspphethrval

859095

aspaspvallyslyslysvalilethrleuasplysprothrasparg

100105110

glugluthrgluaspleuvalglngluphelysalathrcysphetrp

115120125

asnalavalvalgluaspasplysvallysvalthrileargasnlys

130135140

asnargalaileser

145

<210>169

<211>119

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>169

metglnphevalvalthrasnlyssergluleuphelysphealatrp

151015

lysilephelysalaasnlysaspilealapheserglucysleugln

202530

asnalatrppheglntyrlysargtyrleuasnargglualailelys

354045

alaalaglnglnarglysleualalyspheilealaaspthrgluasn

505560

glugluvallysalatrpasntrpalaglulyslysleuglyvalala

65707580

leuasnleuthraspalaglulysgluargasnvalargasnmettyr

859095

lysglumettrpasnalaasnvaltrpalathralailelysalaval

100105110

lysleuhismetgluilegly

115

<210>170

<211>148

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>170

metasngluleuargserleuglumetserileasnalalyslystyr

151015

aspthrargleugluserglyasnargvalleuasnileglyphegly

202530

aspglygluasptyrprovalcysserserserargtyrserleulys

354045

gluserpheileglucysphelysaspglytrpserglythrtyrarg

505560

aspglulysgluleumetgluaspmetglngluilealaglngluleu

65707580

ileleuglugluleuthraspilepheglutyrtyrglupheasnthr

859095

aspgluileaspthraspleuphelysglyphethrpheaspvalasp

100105110

seraspleugluaspsermetalaleumetlysalaileasnalathr

115120125

lystyrpheglualaargsersersertrptyralaserphegluval

130135140

sertyrilegly

145

<210>171

<211>28

<212>rna

<213>人工序列

<220>

<223>合成的crrna间隔子序列

<400>171

gauagcaagauggauaguuaguuuuaga28

<210>172

<211>36

<212>dna

<213>人工序列

<220>

<223>合成的前间区序列(phij0161a原噬菌体))

<400>172

acgctccgtaactatccatcttgctatctgtatatg36

<210>173

<211>36

<212>dna

<213>人工序列

<220>

<223>合成的前间区序列(phij0161a原噬菌体))-互补序列

<400>173

catatacagatagcaagatggatagttacggagcgt36

<210>174

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>174

tggttctttcgctttcacaagatacttttg30

<210>175

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>175

acccgtttctgatgaacgatacactgttgt30

<210>176

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>176

catctatcaagctacaaattggatgtacac30

<210>177

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>177

aagacgaagtgaacgaagagtctttcccag30

<210>178

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>178

taacgtttggaaccctaggcattatgttgc30

<210>179

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>179

gagaatgcaagaattaattaatgagtacag30

<210>180

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>180

tgttgttataaacactgttaactgttgttc30

<210>181

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>181

tctatgccttgatattttaaatgaccgagt30

<210>182

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>182

taagcaagttgcagataagaaaaccatgtc30

<210>183

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>183

ttttgatgatgaaccagaatggatttatta30

<210>184

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>184

ctatccgcaaacacatgttgatggcgtcgt30

<210>185

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>185

aaaaaaatattaacacgaattagatactaa30

<210>186

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>186

caaggcacttttcattttttgaacacgcca30

<210>187

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>187

aactgttgtgccttcctttgcgtgcacttg30

<210>188

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>188

tctttttgatgtattcatttattgttagaa30

<210>189

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b;包括自靶向间隔子

<400>189

agcatatacagatagcaagatggatagtta30

<210>190

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>190

ttcaaggtcaagatcaagagcaagaatgcc30

<210>191

<211>30

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>191

cgtcgcataatactgttcctctgccgttcg30

<210>192

<211>29

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>192

gaatattcttccccccatgactaacaggg29

<210>193

<211>36

<212>dna

<213>人工序列

<220>

<223>合成的间隔子序列，来自图7b

<400>193

gttttagagctatgttattttgaatgctaccaaaac36

<210>194

<211>38

<212>rna

<213>人工序列

<220>

<223>合成的crrna间隔子序列

<400>194

agcauauacagauagcaagauggauaguuaguuuuaga38

<210>195

<211>46

<212>dna

<213>人工序列

<220>

<223>合成的靶dna序列

<400>195

acgctccgtaactatccatcttgctatctgtatatgcttttgcaga46

<210>196

<211>46

<212>dna

<213>人工序列

<220>

<223>合成的靶dna序列(互补序列）

<400>196

tctgcaaaagcatatacagatagcaagatggatagttacggagcgt46

<210>197

<211>154

<212>dna

<213>酿脓链球菌(streptocococcuspyogenes)

<400>197

tatggctgataaatttctttgaatttctccttgattatttgttataaaagttataaaata60

atcttgttggaaccattcaaaacagcatagcaagttaaaataaggctagtccgttatcaa120

cttgaaaaagtggcaccgagtcggtgcttttttt154

<210>198

<211>157

<212>dna

<213>单核细胞增生李斯特菌(listeriamonocytogenes)

<400>198

catattcttcatcccttcaacctgtttttagttgcattctttttataatttggtacaatg60

tatatattgttagtattcaaaataacatagcaagttaaaataaggctttgtccgttatca120

acttttaattaagtagcgctgtttcggcgcttttttt157

<210>199

<211>157

<212>dna

<213>单核细胞增生李斯特菌(listeriamonocytogenes)

<400>199

catattcttcatcccttcaacctgtttttagttgcattctttttataatttggtacaatg60

tatatattgttagtattcaaaataacatagcaagttaaaataaggctttgtccgttatca120

acttttaattaagtagcgctgtttcggcgcttttttt157

<210>200

<211>157

<212>dna

<213>无害李斯特菌(listeriainnocua)

<400>200

catattcttcatcccttcaacctatttttagttgcattctttttataatttggtacaatg60

tatatattgttagtattcaaaataacatagcaagttaaaataaggctttgtccgttatca120

acttttaattaagtagcgctgtttcggcgcttttttt157

<210>201

<211>246

<212>dna

<213>酿脓链球菌(streptocococcuspyogenes)

<400>201

ttagatgaagattatttcttaataactaaaaatatggtataatactcttaataaatgcag60

taatacaggggcttttcaagactgaagtctagctgagacaaatagtgcgattacgaaatt120

ttttagacaaaaatagtctacgaggttttagagctatgctgttttgaatggtcccaaaac180

tgcgctggttgatttcttcttgcgctttttgttttagagctatgctgttttgaatggtcc240

caaaac246

<210>202

<211>233

<212>dna

<213>单核细胞增生李斯特菌(listeriamonocytogenes)

<400>202

taaggtaaccgctgttctttgaaaacaaaataaattttatgtaaaccataaaatagcatt60

caaaattgaaatcttgctatggatgaatggcgcgattacggaatcttggaggaaagaaaa120

aattctgcgaggttttagagctatgttattttgaatgctaccaaaactcgtgatttcttc180

ctcatgcgcgcttttgagttttagagctatgttattttgaatgctaccaaaac233

<210>203

<211>233

<212>dna

<213>单核细胞增生李斯特菌(listeriamonocytogenes)

<400>203

taaggtaaccgctgttctttgaaaacaaaataaattttatgtaaaccataaaatagcatt60

caaaattgaaatcttgctatggatgaatggcgcgattacggaatcttggaggaaagaaaa120

aattctgcgaggttttagagctatgttattttgaatgctaccaaaactggttctttcgct180

ttcacaagatacttttggttttagagctatgttattttgaatgctaccaaaac233

<210>204

<211>231

<212>dna

<213>无害李斯特菌(listeriainnocua)

<400>204

tagagttcaaatgatctttgaaaactaaatagattttatgtgaaccataaaataagcatt60

caaaattgaaatcttgctatggatgaatggcgcgattacgaaatctaggagaataaaaaa120

ttctacgaggttttagagctatgttattttgaatgctaacaaaaccgtagcgctttggta180

actttgcctaggatagttttagagctatgttattttgaatgctaacaaaac231

<210>205

<211>1368

<212>prt

<213>酿脓链球菌(streptocococcuspyogenes)

<400>205

metasplyslystyrserileglyleuaspileglythrasnserval

151015

glytrpalavalilethraspglutyrlysvalproserlyslysphe

202530

lysvalleuglyasnthrasparghisserilelyslysasnleuile

354045

glyalaleuleupheaspserglygluthralaglualathrargleu

505560

lysargthralaargargargtyrthrargarglysasnargilecys

65707580

tyrleuglngluilepheserasnglumetalalysvalaspaspser

859095

phephehisargleuglugluserpheleuvalglugluasplyslys

100105110

hisgluarghisproilepheglyasnilevalaspgluvalalatyr

115120125

hisglulystyrprothriletyrhisleuarglyslysleuvalasp

130135140

serthrasplysalaaspleuargleuiletyrleualaleualahis

145150155160

metilelyspheargglyhispheleuilegluglyaspleuasnpro

165170175

aspasnseraspvalasplysleupheileglnleuvalglnthrtyr

180185190

asnglnleupheglugluasnproileasnalaserglyvalaspala

195200205

lysalaileleuseralaargleuserlysserargargleugluasn

210215220

leuilealaglnleuproglyglulyslysasnglyleupheglyasn

225230235240

leuilealaleuserleuglyleuthrproasnphelysserasnphe

245250255

aspleualagluaspalalysleuglnleuserlysaspthrtyrasp

260265270

aspaspleuaspasnleuleualaglnileglyaspglntyralaasp

275280285

leupheleualaalalysasnleuseraspalaileleuleuserasp

290295300

ileleuargvalasnthrgluilethrlysalaproleuseralaser

305310315320

metilelysargtyraspgluhishisglnaspleuthrleuleulys

325330335

alaleuvalargglnglnleuproglulystyrlysgluilephephe

340345350

aspglnserlysasnglytyralaglytyrileaspglyglyalaser

355360365

glnglugluphetyrlyspheilelysproileleuglulysmetasp

370375380

glythrglugluleuleuvallysleuasnarggluaspleuleuarg

385390395400

lysglnargthrpheaspasnglyserileprohisglnilehisleu

405410415

glygluleuhisalaileleuargargglngluaspphetyrprophe

420425430

leulysaspasnargglulysileglulysileleuthrpheargile

435440445

protyrtyrvalglyproleualaargglyasnserargphealatrp

450455460

metthrarglysserglugluthrilethrprotrpasnphegluglu

465470475480

valvalasplysglyalaseralaglnserpheilegluargmetthr

485490495

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500505510

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515520525

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530535540

lyslysalailevalaspleuleuphelysthrasnarglysvalthr

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565570575

servalgluileserglyvalgluaspargpheasnalaserleugly

580585590

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595600605

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610615620

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625630635640

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645650655

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660665670

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675680685

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690695700

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705710715720

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725730735

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740745750

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755760765

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785790795800

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805810815

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820825830

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835840845

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850855860

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865870875880

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885890895

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900905910

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915920925

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930935940

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945950955960

lysleuvalseraspphearglysasppheglnphetyrlysvalarg

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980985990

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99510001005

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101010151020

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108510901095

gluvalglnthrglyglypheserlysgluserileleuprolys

110011051110

argasnserasplysleuilealaarglyslysasptrpasppro

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lyslystyrglyglypheaspserprothrvalalatyrserval

113011351140

leuvalvalalalysvalglulysglylysserlyslysleulys

114511501155

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pheglulysasnproileasppheleuglualalysglytyrlys

117511801185

gluvallyslysaspleuileilelysleuprolystyrserleu

119011951200

phegluleugluasnglyarglysargmetleualaseralagly

120512101215

gluleuglnlysglyasngluleualaleuproserlystyrval

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125012551260

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126512701275

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128012851290

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129513001305

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131013151320

phelystyrpheaspthrthrileasparglysargtyrthrser

132513301335

thrlysgluvalleuaspalathrleuilehisglnserilethr

134013451350

glyleutyrgluthrargileaspleuserglnleuglyglyasp

135513601365

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metlysasnprotyrthrileglyleuaspileglythrasnserval

151015

glytrpalavalleuthrasnglntyraspleuvallysarglysmet

202530

lysvalalaglyasnserasplyslysglnilelyslysasnphetrp

354045

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505560

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130135140

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145150155160

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165170175

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180185190

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195200205

glugluasnilegluvalalaasnileleualaglylysphethrarg

210215220

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225230235240

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245250255

asnpheglnlysvalpheaspleuileglulysthraspileglucys

260265270

alalysaspsertyrglugluaspleuglualaleuleualaileile

275280285

glyaspglutyralagluleuphevalalaalalysasnthrtyrasn

290295300

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305310315320

alalysleuseralasermetilegluargpheaspalahisglulys

325330335

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340345350

tyrglngluilepheasnasnalaalaileaspglytyralaglytyr

355360365

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370375380

ileleugluasnilegluglyalaasptyrpheilealalysileglu

385390395400

glugluasnpheleuarglysglnargthrpheaspasnglyalaile

405410415

prohisglnleuhisleuglugluleuglualaileilehisglngln

420425430

alalystyrtyrpropheleuarggluasptyrglulysilelysser

435440445

leuvalthrpheargileprotyrphevalglyproleualalysgly

450455460

glnsergluphealatrpleuthrarglysalaaspglygluilearg

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protrpasnilegluglulysvalasppheglylysseralavalasp

485490495

pheileglulysmetthrasnlysaspthrtyrleuprolysgluasn

500505510

valleuprolyshisserleucystyrglnlystyrmetvaltyrasn

515520525

gluleuthrlysvalargtyrileaspaspglnglylysthrasntyr

530535540

pheserglyglnglulysglnglnilepheasnaspleuphelysgln

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lysarglysvallyslyslysaspleugluleupheleuargasnile

565570575

asnhisilegluserprothrilegluglyleugluaspserpheasn

580585590

alasertyralathrtyrhisaspleuleulysvalglymetlysgln

595600605

gluileleuaspasnproleuasnthrglumetleugluaspileval

610615620

lysileleuthrvalphegluasplysprometilelysgluglnleu

625630635640

glnglnpheseraspvalleuaspglyglyvalleulyslysleuglu

645650655

argarghistyrthrglytrpglyargleuseralalysleuleuval

660665670

glyileargglulysglnserhisleuthrileleuasptyrleumet

675680685

asnaspaspglyleuasnargasnleumetglnleuileasnaspser

690695700

asnleuserphelysserileileglulysgluglnvalserthrthr

705710715720

asplysaspleuglnserilevalalaaspleualaglyserproala

725730735

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740745750

serilemetglytyrproproglnthrilevalvalglumetalaarg

755760765

gluasnglnthrthrglylysglylysasnasnserlysproargtyr

770775780

lysserleuglulysalailelysglupheglyserlysileleulys

785790795800

gluhisprothraspasnglngluleulysasnasnargleutyrleu

805810815

tyrtyrleuglnasnglylysaspmettyrthrglyglngluleuasp

820825830

ilehisasnleuserasntyraspileasphisilevalproglnser

835840845

pheilethraspasnserileaspasnleuvalleuthrserserala

850855860

glyasnargglulysglyglyaspvalproproleugluilevalarg

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lysarglysvalphetrpglulysleutyrglnglyasnleumetser

885890895

lysarglyspheasptyrleuthrlysalagluargglyglyleuthr

900905910

aspalaasplysalaargpheilehisargglnleuvalgluthrarg

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glnilethrlysasnvalalaasnileleuhisglnargpheasnasn

930935940

gluthraspasnhisglyasnthrmetgluglnvalargilevalthr

945950955960

leulysseralaleuvalserglnphearglysglnpheglnleutyr

965970975

lysvalarggluvalasnasptyrhishisalahisaspalatyrleu

980985990

asnglyvalvalalaasnthrleuleulysvaltyrproglnleuglu

99510001005

progluphevaltyrglyglutyrhisglnpheasptrpphelys

101010151020

alaasnlysalathralalyslysglnphetyrthrasnilemet

102510301035

leuphepheglyglnlysgluargileileaspgluasnglyglu

104010451050

ileleutrpasplyslystyrleugluthrilelyslysvalleu

105510601065

asptyrargglnmetasnilevallyslysthrgluileglnlys

107010751080

glyglupheserlysalathrilelysprolysglyasnserser

108510901095

lysleuileproarglysgluasntrpaspprometlystyrgly

110011051110

glyleuaspserproasnmetalatyralavalileilegluhis

111511201125

alalysglylyslyslysleuilepheglulyslysileilearg

113011351140

ilethrilemetgluarglysmetpheglulysaspglugluala

114511501155

pheleugluglulysglytyrarghisprolysvalleuthrlys

116011651170

leuprolystyrthrleutyrglucysglulysglyargargarg

117511801185

metleualaseralaasnglualaglnlysglyasnglnleuval

119011951200

leuserasnhisleuvalserleuleutyrhisalalysasncys

120512101215

glualaseraspglylysserleulystyrileglualahisarg

122012251230

gluthrphesergluleuleualaglnvalsergluphealathr

123512401245

argtyrthrleualaaspalaasnleuserlysileasnasnleu

125012551260

phegluglnasnlysgluglyaspileglnalailealaglnser

126512701275

phevalaspleumetalapheasnalametglyalaproalaser

128012851290

phelysphepheglualathrileasparglysargtyrthrasn

129513001305

leulysgluleuleuserserthrileiletyrglnserilethr

131013151320

glyleutyrgluserarglysargleuaspasp

13251330

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<400>207

metlysasnprotyrthrileglyleuaspileglythrasnserval

151015

glytrpalavalleuthrasnglntyraspleuvallysarglysmet

202530

lysvalalaglyasnserasplyslysglnilelyslysasnphetrp

354045

glyvalargleupheaspaspglyglnthralavalaspargargmet

505560

asnargthralaargargargilegluargargargasnargileser

65707580

tyrleuglngluilephealavalglumetalaasnileaspalaasn

859095

phephecysargleuasnaspserphetyrvalaspserglulysarg

100105110

asnserarghisprophephealathrilegluglugluvalalatyr

115120125

hisaspasntyrargthriletyrhisleuargglulysleuvalasn

130135140

serserglulysalaaspleuargleuvaltyrleualaleualahis

145150155160

ileilelystyrargglyasnpheleuilegluglyalaleuaspthr

165170175

lysasnthrservalaspgluvaltyrlysglnpheilegluthrtyr

180185190

asnglnvalphemetserasnileglugluglyalaleualalysval

195200205

glugluasnilegluvalalaasnileleualaglylysphethrarg

210215220

argglulysphegluargileleuglnleutyrproglyglulysser

225230235240

thrglymetphealaglnpheileserleuilevalglyserlysgly

245250255

asnpheglnlysvalpheaspleuileglulysthraspileglucys

260265270

alalysaspsertyrglugluaspleugluthrleuleualaileile

275280285

glyaspglutyralagluleuphevalalaalalysasnthrtyrasn

290295300

alavalvalleuserserileilethrvalthraspthrgluthrasn

305310315320

alalysleuseralasermetilegluargpheaspalahisglulys

325330335

aspleuvalgluleulysalapheilelysleuasnleuprolysgln

340345350

tyrglugluilepheserasnalaalaileaspglytyralaglytyr

355360365

ileaspglylysthrlysglnvalaspphetyrlystyrleulysthr

370375380

ileleugluasnilegluglyserasptyrpheilealalysileglu

385390395400

glugluasnpheleuarglysglnargthrpheaspasnglyalaile

405410415

prohisglnleuhisleuglugluleuglualaileilehisglngln

420425430

alalystyrtyrpropheleulysgluasptyrasplysilelysser

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leuvalthrpheargileprotyrphevalglyproleualaasngly

450455460

glnsergluphealatrpleuthrarglysalaaspglygluilearg

465470475480

protrpasnilegluglulysvalasppheglylysseralavalasp

485490495

pheileglulysmetthrasnlysaspthrtyrleuprolysgluasn

500505510

valleuprolyshisserleucystyrglnlystyrmetvaltyrasn

515520525

gluleuthrlysileargtyrileaspaspglnglylysthrasntyr

530535540

pheserglyargglulysglnglnvalpheasnaspleuphelysgln

545550555560

lysarglysvallyslyslysaspleugluleupheleuargasnile

565570575

asnhisilegluserprothrilegluglyleugluaspserpheasn

580585590

alasertyralathrtyrhisaspleuleulysvalglymetlysgln

595600605

gluileleuaspasnproleuasnthrglumetleugluaspileval

610615620

lysileleuthrvalphegluasplysprometilelysgluglnleu

625630635640

glnglnpheseraspvalleuaspglyglyvalleulyslysleuglu

645650655

argarghistyrthrglytrpglyargleuseralalysleuleuval

660665670

glyileargglulysglnserhisleuthrileleuasptyrleumet

675680685

asnaspaspglyleuasnargasnleumetglnleuileasnaspser

690695700

asnleuserphelysserileileglulysgluglnvalserthrthr

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asplysaspleuglnserilevalalagluleualaglyserproala

725730735

ilelyslysglyileleuglnserleulysilevalaspgluleuval

740745750

serilemetglytyrproproglnthrilevalvalglumetalaarg

755760765

gluasnglnthrthrglylysglylysasnasnserlysproargtyr

770775780

lysserleuglulysalailelysglupheglyserglnileleulys

785790795800

gluhisprothraspasnglngluleulysasnasnargleutyrleu

805810815

tyrtyrleuglnasnglylysaspmettyrthrglyglngluleuasp

820825830

ilehisasnleuserasntyraspileasphisilevalproglnser

835840845

pheilethraspasnserileaspasnleuvalleuthrserserala

850855860

glyasnargglulysglyglyaspvalproproleugluilevalarg

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lysarglysvalphetrpglulysleutyrglnglyasnleumetser

885890895

lysarglyspheasptyrleuthrlysalagluargglyglyleuthr

900905910

glualaasplysalaargpheilehisargglnleuvalgluthrarg

915920925

glnilethrlysasnvalalaasnileleutyrglnargpheasnlys

930935940

gluthraspasnhisglyasnthrmetgluglnvalargilevalthr

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leulysseralaleuvalserglnphearglysglnpheglnleutyr

965970975

lysvalarggluvalasnglytyrhishisalahisaspalatyrleu

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asnglyvalvalalaasnthrleuleulysvaltyrproglnleuglu

99510001005

progluphevaltyrglyglutyrhisglnpheasptrpphelys

101010151020

alaasnlysalathralalyslysglnphetyrthrasnilemet

102510301035

leuphephealaglnlysgluargileileaspgluasnglyglu

104010451050

ileleutrpasplyslystyrleugluthrilelyslysvalleu

105510601065

asptyrargglnmetasnilevallyslysthrgluileglnlys

107010751080

glyglupheserlysalathrilelysprolysglyasnserser

108510901095

lysleuileproarglysgluasntrpaspprometlystyrgly

110011051110

glyleuaspserproasnmetalatyralavalileilegluhis

111511201125

alalysglylyslyslysilevalileglulyslysleuilegln

113011351140

ileasnilemetgluarglysmetpheglulysaspglugluala

114511501155

pheleugluglulysglytyrarghisprolysvalleuthrlys

116011651170

leuprolystyrthrleutyrglucysglulysglyargargarg

117511801185

metleualaseralaasnglualaglnlysglyasnglnleuval

119011951200

leuserasnhisleuvalserleuleutyrhisalalysasncys

120512101215

glualaseraspglylysserleulystyrileglualahisarg

122012251230

gluthrphesergluleuleualaglnvalsergluphealathr

123512401245

argtyrthrleualaaspalaasnleuserlysileasnasnleu

125012551260

phegluglnasnlysgluglyaspilelysalailealaglnser

126512701275

phevalaspleumetalapheasnalametglyalaproalaser

128012851290

phelysphepheglualathrileasparglysargtyrthrasn

129513001305

leulysgluleuleuserserthrileiletyrglnserilethr

131013151320

glyleutyrgluserarglysargleuaspasp

13251330

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metlyslysprotyrthrileglyleuaspileglythrasnserval

151015

glytrpalavalleuthraspglntyraspleuvallysarglysmet

202530

lysilealaglyaspserglulyslysglnilelyslysasnphetrp

354045

glyvalargleupheaspgluglyglnthralaalaaspargargmet

505560

alaargthralaargargargilegluargargargasnargileser

65707580

tyrleuglnglyilephealagluglumetserlysthraspalaasn

859095

phephecysargleuseraspserphetyrvalaspasnglulysarg

100105110

asnserarghisprophephealathrilegluglugluvalglutyr

115120125

hislysasntyrprothriletyrhisleuargglugluleuvalasn

130135140

serserglulysalaaspleuargleuvaltyrleualaleualahis

145150155160

ileilelystyrargglyasnpheleuilegluglyalaleuaspthr

165170175

glnasnthrservalaspglyiletyrlysglnpheileglnthrtyr

180185190

asnglnvalphealaserglyilegluaspglyserleulyslysleu

195200205

gluaspasnlysaspvalalalysileleuvalglulysvalthrarg

210215220

lysglulysleugluargileleulysleutyrproglyglulysser

225230235240

alaglymetphealaglnpheileserleuilevalglyserlysgly

245250255

asnpheglnlyspropheaspleuileglulysseraspileglucys

260265270

alalysaspsertyrglugluaspleugluserleuleualaleuile

275280285

glyaspglutyralagluleuphevalalaalalysasnalatyrser

290295300

alavalvalleuserserileilethrvalalagluthrgluthrasn

305310315320

alalysleuseralasermetilegluargpheaspthrhisgluglu

325330335

aspleuglygluleulysalapheilelysleuhisleuprolyshis

340345350

tyrglugluilepheserasnthrglulyshisglytyralaglytyr

355360365

ileaspglylysthrlysglnalaaspphetyrlystyrmetlysmet

370375380

thrleugluasnilegluglyalaasptyrpheilealalysileglu

385390395400

lysgluasnpheleuarglysglnargthrpheaspasnglyalaile

405410415

prohisglnleuhisleuglugluleuglualaileleuhisglngln

420425430

alalystyrtyrpropheleulysgluasntyrasplysilelysser

435440445

leuvalthrpheargileprotyrphevalglyproleualaasngly

450455460

glnsergluphealatrpleuthrarglysalaaspglygluilearg

465470475480

protrpasnilegluglulysvalasppheglylysseralavalasp

485490495

pheileglulysmetthrasnlysaspthrtyrleuprolysgluasn

500505510

valleuprolyshisserleucystyrglnlystyrleuvaltyrasn

515520525

gluleuthrlysvalargtyrileasnaspglnglylysthrsertyr

530535540

pheserglyglnglulysgluglnilepheasnaspleuphelysgln

545550555560

lysarglysvallyslyslysaspleugluleupheleuargasnmet

565570575

serhisvalgluserprothrilegluglyleugluaspserpheasn

580585590

sersertyrserthrtyrhisaspleuleulysvalglyilelysgln

595600605

gluileleuaspasnprovalasnthrglumetleugluasnileval

610615620

lysileleuthrvalphegluasplysargmetilelysgluglnleu

625630635640

glnglnpheseraspvalleuaspglyvalvalleulyslysleuglu

645650655

argarghistyrthrglytrpglyargleuseralalysleuleumet

660665670

glyileargasplysglnserhisleuthrileleuasptyrleumet

675680685

asnaspaspglyleuasnargasnleumetglnleuileasnaspser

690695700

asnleuserphelysserileileglulysgluglnvalthrthrala

705710715720

asplysaspileglnserilevalalaaspleualaglyserproala

725730735

ilelyslysglyileleuglnserleulysilevalaspgluleuval

740745750

servalmetglytyrproproglnthrilevalvalglumetalaarg

755760765

gluasnglnthrthrglylysglylysasnasnserargproargtyr

770775780

lysserleuglulysalailelysglupheglyserglnileleulys

785790795800

gluhisprothraspasnglngluleuargasnasnargleutyrleu

805810815

tyrtyrleuglnasnglylysaspmettyrthrglyglnaspleuasp

820825830

ilehisasnleuserasntyraspileasphisilevalproglnser

835840845

pheilethraspasnserileaspasnleuvalleuthrserserala

850855860

glyasnargglulysglyaspaspvalproproleugluilevalarg

865870875880

lysarglysvalphetrpglulysleutyrglnglyasnleumetser

885890895

lysarglyspheasptyrleuthrlysalagluargglyglyleuthr

900905910

glualaasplysalaargpheilehisargglnleuvalgluthrarg

915920925

glnilethrlysasnvalalaasnileleuhisglnargpheasntyr

930935940

glulysaspasphisglyasnthrmetlysglnvalargilevalthr

945950955960

leulysseralaleuvalserglnphearglysglnpheglnleutyr

965970975

lysvalargaspvalasnasptyrhishisalahisaspalatyrleu

980985990

asnglyvalvalalaasnthrleuleulysvaltyrproglnleuglu

99510001005

progluphevaltyrglyasptyrhisglnpheasptrpphelys

101010151020

alaasnlysalathralalyslysglnphetyrthrasnilemet

102510301035

leuphephealaglnlysaspargileileaspgluasnglyglu

104010451050

ileleutrpasplyslystyrleuaspthrvallyslysvalmet

105510601065

sertyrargglnmetasnilevallyslysthrgluileglnlys

107010751080

glyglupheserlysalathrilelysprolysglyasnserser

108510901095

lysleuileproarglysthrasntrpaspprometlystyrgly

110011051110

glyleuaspserproasnmetalatyralavalvalileglutyr

111511201125

alalysglylysasnlysleuvalpheglulyslysileilearg

113011351140

valthrilemetgluarglysalapheglulysaspglulysala

114511501155

pheleuglugluglnglytyrargglnprolysvalleualalys

116011651170

leuprolystyrthrleutyrglucysglugluglyargargarg

117511801185

metleualaseralaasnglualaglnlysglyasnglnglnval

119011951200

leuproasnhisleuvalthrleuleuhishisalaalaasncys

120512101215

gluvalseraspglylysserleuasptyrilegluserasnarg

122012251230

glumetphealagluleuleualahisvalsergluphealalys

123512401245

argtyrthrleualaglualaasnleuasnlysileasnglnleu

125012551260

phegluglnasnlysgluglyaspilelysalailealaglnser

126512701275

phevalaspleumetalapheasnalametglyalaproalaser

128012851290

phelysphephegluthrthrilegluarglysargtyrasnasn

129513001305

leulysgluleuleuasnserthrileiletyrglnserilethr

131013151320

glyleutyrgluserarglysargleuaspasp

13251330

<210>209

<211>132

<212>prt

<213>未知

<220>

<223>未知细菌,未知病毒(噬菌体)或合成的

cas9抑制性多肽

<400>209

metglymetthrthralaarglyslysphetyrglnalaileserglu

151015

pheglualametthrglylysaspvalgluargthrproglnileala

202530

aspgluvalleuasnaspalaglutyrilealaphethrlysthrglu

354045

lystyralaleutyrleucysthrserasnvalgluglyleugluasp

505560

argtyrpheleuaspgluglucysleuaspserthrpheleugluthr

65707580

gluaspasngluthrtyrtyrilehispheleuglngluthrgluphe

859095

sergluaspaspasngluaspgluleuproleualathrgluglugln

100105110

ileglualatyrasplysglnglugluleulysalavalileleulys

115120125

lysgluleuasn

130