ys Phe Ser Asp Asp Glu lie Asp Leu Leu lie Arg Leu His Lys Leu65707580
cta ggg aac agg tgg tcg ctg ate gcg ggc aga ate ccc ggt agg aca289
Leu Gly Asn Arg Trp Ser Leu lie Ala Gly Arg lie Pro Gly Arg Thr859095
gcg aac gac ate aag aat tat tgg aac tct cac ctg age aag aga gtg337
Ala Asn Asp lie Lys Asn Tyr Trp Asn Ser His Leu Ser Lys Arg Val100105110
gaa gca aca aaa tgt agg age aaa act ata gat get caa gtc gcg agg385
Glu Ala Thr Lys Cys Arg Ser Lys Thr lie Asp Ala Gln Val Ala Arg 115120125
cca aat cct gag aga gat tcc gcc aac tgg agt tgg cca aca aat ata433
Pro Asn Pro Glu Arg Asp Ser Ala Asn Trp Ser Trp Pro Thr Asn lie130135140
gga aca aat tat ggg aac aac ttg gaa gca gaa gaa acc gaa atg ccg481
Gly Thr Asn Tyr Gly Asn Asn Leu Glu Ala Glu Glu Thr Glu Met Pro145150155160
aaa cta cga aca agt caa gag aat cag gga gaa egg tea aat gat ttg529
Lys Leu Arg Thr Ser Gln Glu Asn Gln Gly Glu Arg Ser Asn Asp Leu165170175
ate atg gag aat ggt tgg gaa gag ttg atg ctg cat act tct gac gtg577Ile Met Glu Asn Gly Trp Glu Glu Leu Met Leu His Thr Ser Asp Val180185190
age tta cag aat ttt cag ata gac ttc aag aca ccg gaa atg gag gaa625
Ser Leu Gln Asn Phe Gln lie Asp Phe Lys Thr Pro Glu Met Glu Glu 195200205
cta act gag atg gag gaa cta act gag gaa cca ate tct ctg gga gat673
Leu Thr Glu Met Glu Glu Leu Thr Glu Glu Pro lie Ser Leu Gly Asp210215220
gaa ggg aat gat tgg act gcc atg gaa aac tat ttt ttt gat gag ctc721
Glu Gly Asn Asp Trp Thr Ala Met Glu Asn Tyr Phe Phe Asp Glu Leu225230235240
att agt taa gaaaaaaata tatactaaaa tgcgg755
lie Ser〈210〉 2〈211〉 242〈212〉 PRT<213> Freesia alba〈400〉 2
Met Asp Leu Lys Ala Ser Ala Leu Arg Lys Gly Ala Trp Ser Lys Glu151015
Glu Asp Asp Leu Leu Arg Asn Cys lie Asp Lys Tyr Gly Glu Gly Lys202530
Trp Ser Ser Val Pro Glu Arg Ala Gly Leu Lys Arg Cys Arg Lys Ser 354045
Cys Arg Leu Arg Trp Leu Asn Tyr Leu Ser Pro Lys lie Asn Arg Gly505560
Lys Phe Ser Asp Asp Glu lie Asp Leu Leu lie Arg Leu His Lys Leu65707580
Leu Gly Asn Arg Trp Ser Leu lie Ala Gly Arg lie Pro Gly Arg Thr859095
Ala Asn Asp lie Lys Asn Tyr Trp Asn Ser His Leu Ser Lys Arg Val100105110
Glu Ala Thr Lys Cys Arg Ser Lys Thr lie Asp Ala Gln Val Ala Arg 115120125
Pro Asn Pro Glu Arg Asp Ser Ala Asn Trp Ser Trp Pro Thr Asn lie130135140
Gly Thr Asn Tyr Gly Asn Asn Leu Glu Ala Glu Glu Thr Glu Met Pro145150155160
Lys Leu Arg Thr Ser Gln Glu Asn Gln Gly Glu Arg Ser Asn Asp Leu165170175
lie Met Glu Asn Gly Trp Glu Glu Leu Met Leu His Thr Ser Asp Val180185190
Ser Leu Gln Asn Phe Gln lie Asp Phe Lys Thr Pro Glu Met Glu Glu 195200205
Leu Thr Glu Met Glu Glu Leu Thr Glu Glu Pro lie Ser Leu Gly Asp210215220
Glu Gly Asn Asp Trp Thr Ala Met Glu Asn Tyr Phe Phe Asp Glu Leu225230235240
lie Ser 0
【主权项】
1.正调控香雪兰花朵花色苷合成的MYB转录因子的CDNA序列，其特征是: 其中1:/?/?/5/"基因的全长核苷酸序列(SEQ ID N0.1)ATCCAATATTCCTCCCCCTAATGTCTTATCTTGCATGATTCACAATTATGAAACATCAGTACTGCTCCGAGCTCCGACCTTCGGGCGTTCAGAATCGGAAAGGGGCATGGAACAAAGATGAAGATGAGCTGCTGAGGAAGTGCATTGAAAAGTATGGAATAGGGAAGTGGAGTACAGTTCCCATGAGGGCAGGACTAAGAAGGTGTCGCAAGAGCTGTCGTCTTCGCTGGCTGAACTATCTATCCCCTGACATCAACCGAGGAACCTTTAACGACGACGAAGACGACCTCATCCTTCGGCTTCACAAGCTCTTGGGCAATAGGTGGTCCCTAATTGCAGGCAGAATCCCCGGCAGGACGGCGAACGACATCAAGAACTACTGGAACTCTCACTTGGGCAAGAAAGCAGACACCGAGAGAAGGGTACCAGAGAAGACTGACAAGGCTGAGATTGTAAGGCCACAACCTCAAAGACCGACTTCGACGTGGATTTGGCCGAAGATTAATATTAATCCTCATCAAGAAACTGATCAATTAGGAACATCTGCCGTGGAGCCAAAACTGCCAACAGTCTTAGAGGAGGAAGAAGCATGGCTGAATGCTTTGATCACCGATGATTACAATGAGAATGTGGATGCCGCCTTTTCGGACTTCCATGGCTACGAGATAGGGGGAGGAATATTGGATGACACGCCTTTTTTCGAAGGAGTTACAGGATGGGAGGAACTATACCAGAGAACTGAAAATTAAGTTTAATTTTTCATTGTTCTAACAATACTGATATCTATATATATATTTTTTTTTTCCTTCGAAGATCAAAGATAGAAAGAAATGAGACCAGTTCCCCATTGTTGAAAAATAAAGGAATAAGGTTTATGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGATGTCGACTCGAGTC ； 其中2:由/?/?/5/"基因核苷酸序列推测得到的蛋白质序列(SEQ ID N0.2)MKHQYCSELRPSGVQNRKGAffNKDEDELLRKCIEKYGIGKWSTVPMRAGLRRCRKSCRLRWLNYLSPDINRGTFNDDEDDLILRLHKLLGNRWSLIAGRIPGRTANDIKNYWNSHLGKKADTERRVPEKTDKAEIVRPQPQRPTSTWIffPKININPHQETDQLGTSAVEPKLPTVLEEEEAWLNALITDDYNENVDAAFSDFHGYEIGGGILDDTPFFEGVTGWEELYQRTEN ； 其中3因的全长核苷酸序列(SEQ ID N0.3)AATGGATCTCAAAGCTTCAGCCCTTCGAAAAGGCGCATGGAGCAAAGAGGAAGACGACCTGCTAAGGAATTGTATCGATAAGTATGGAGAAGGGAAGTGGAGTTCCGTACCCGAACGTGCAGGGCTTAAAAGGTGTCGAAAGAGCTGTCGACTTAGATGGCTGAACTATCTTTCGCCGAAGATCAACCGAGGCAAGTTTTCGGATGACGAGATCGACCTCCTTATCAGGCTTCACAAGCTTCTAGGGAACAGGTGGTCGCTGATCGCGGGCAGAATCCCCGGTAGGACAGCGAACGACATCAAGAATTATTGGAACTCTCACCTGAGCAAGAGAGTGGAAGCAACAAAATGTAGGAGCAAAACTATAGATGCTCAAGTCGCGAGGCCAAATCCTGAGAGAGATTCCGCCAACTGGAGTTGGCCAACAAATATAGGAACAAATTATGGGAACAACTTGGAAGCAGAAGAAACCGAAATGCCGAAACTACGAACAAGTCAAGAGAATCAGGGAGAACGGTCAAATGATTTGATCATGGAGAATGGTTGGGAAGAGTTGATGCTGCATACTTCTGACGTGAGCTTACAGAATTTTCAGATAGACTTCAAGACACCGGAAATGGAGGAACTAACTGAGATGGAGGAACTAACTGAGGAACCAATCTCTCTGGGAGATGAAGGGAATGATTGGACTGCCATGGAAAACTATTTTTTTGATGAGCTCATTAGTTAAGAAAAAAATATATACTAAAATGCGG ； 其中4:由APUZiS因核苷酸序列推测得到的蛋白质序列(SEQ ID N0.4)MDLKASALRKGAffSKEEDDLLRNCIDKYGEGKWSSVPERAGLKRCRKSCRLRWLNYLSPKINRGKFSDDEIDLLIRLHKLLGNRWSLIAGRIPGRTANDIKNYWNSHLSKRVEATKCRSKTIDAQVARPNPERDSANWSWPTNIGTNYGNNLEAEETEMPKLRTSQENQGERSNDLIMENGWEELMLHTSDVSLQNFQIDFKTPEMEELTEMEELTEEPISLGDEGNDWTAMENYFFDELIS。
【专利摘要】本发明首次从红花香雪兰花瓣中克隆得到两条正调控花色苷合成的MYB转录因子(<i>FhPAP1L1</i>和<i>FhPAP1L2</i>)的cDNA序列，并推测得到了其编码的氨基序列。在拟南芥和烟草中过量表达<i>FhPAP1L1</i>和<i>FhPAP1L2</i>可以明显地增强植物组织的着色，从而确定其参与正调控植物花色苷合成的功能。
【IPC分类】C12N15/29, C07K14/415
【公开号】CN105132433
【申请号】CN201510403554
【发明人】高翔, 王丽, 李月庆, 单晓彤
【申请人】东北师范大学
【公开日】2015年12月9日
【申请日】2015年7月12日