用于编码精细化学品的代谢途径的蛋白质的基因变体的制作方法

专利名称：：用于编码精细化学品的代谢途径的蛋白质的基因变体的制作方法
技术领域：
：本发明关于精细化学品的代谢途径的突变核酸及蛋白质、制备经基因修饰生产者生物的方法、通过培养该经基因修饰生物制备精细化学品的方法以及该经基因修饰生物本身。细胞中天然存在代谢过程的许多产物及副产品可以用于许多工业部门，包括食品业、动物饲料业、化妆品业及制药业。此些化合物统称为“精细化学品”，其包含例如有机酸、生蛋白氨基酸(proteinogenicaminoacid)及非生蛋白氨基酸(non-proteinogenicaminoacid)、核苷酸及核苷、脂类及脂肪酸、二元醇、碳水化合物、芳族化合物、维生素及辅因子以及酶。例如，可通过已发展成能够产生和分泌大量一种或多种目的精细化学品的微生物的大规模发酵来生产它们。特别适于此目的生物为谷氨酸棒杆菌(Corynebacteriumglutamicum)，其为革兰氏阳性非致病菌。利用菌种选育，已发展出能生产多种目的化合物的突变菌株。然而，选育在特定化合物产生方面改良的菌种是费时且困难的过程。通过基因修饰能够提高生产者生物的生产力。例如，生产者生物中特定基因的特殊突变可能造成目的精细化学品生产力的提高。欧洲专利EP1108790A2描述了编码高丝氨酸脱氢酶的突变核酸序列，其由编码谷氨酸棒杆菌高丝氨酸脱氢酶的野生型序列开始，与野生型序列相比其具有突变Val59Ala。此外，还描述了编码与谷氨酸棒杆菌野生型氨基酸序列相比具有突变Pro458Ser的丙酮酸羧化酶的突变核酸序列。向谷氨酸棒杆菌中导入该突变导致赖氨酸产量增加。世界专利WO0063388此外还描述了一种突变的ask基因，其编码具有突变T311I的天冬氨酸激酶。在谷氨酸棒杆菌中精细化学品生物合成途径的基因及蛋白质中的其它突变描述于世界专利WO0340681、WO0340357、WO0340181、WO0340293、WO0340292、WO0340291、WO0340180、WO0340290、WO0346123、WO0340289及WO0342389中。虽然先前技术中已知的突变已导致产生具有最佳生产力的生产者生物，即目的精细化学品的最佳产量及最佳C产量，但在进一步改善该生物生产力方面仍有持续不断的需求。本发明的一个目的是提供另外的突变的基因及蛋白质，其导致生产者生物生产精细化学品生产力的提高并且因而导致制备精细化学品的生物工程方法的改进。我们已发现，通过具有表1/第7列所指定的每种情况下的功能以及具有下列所述氨基酸序列的蛋白质来实现该目的，所述氨基酸序列即在从表1/第2列每一情况下所提到的氨基酸序列开始的、对应于表1/第4列中对该氨基酸序列所指定的氨基酸位置中的至少一个位置上具有与表1/第5列同一行中所指定的特定氨基酸不同的生蛋白氨基酸，其前提条件是根据表2的蛋白质除外。本发明提供新的核酸分子和蛋白质，一方面其可用于鉴定谷氨酸棒杆菌或相关细菌物种或将其分类，另一方面其导致生产者生物生产精细化学品生产力的提高并且因而导致制备精细化学品的生物工程方法的改进。谷氨酸棒杆菌为革兰氏阳性好氧细菌，其广泛用于大量精细化学品的大规模生产工业中，也用于碳氢化合物的降解(如在原油泄漏的情况下)和类萜类的氧化。因此，核酸分子还可用于鉴定用于通过例如发酵方法生产精细化学品的微生物。虽然谷氨酸棒杆菌本身为非致病菌，然而，其与其它棒杆菌物种如人类主要病原体白喉棒杆菌(Corynebacteriumdiphtheriae)(白喉病原体)有关。因此鉴定棒杆菌物种存在的能力也具有明显的临床意义，例如应用于诊断。此外，该核酸分子可作为谷氨酸棒杆菌基因组或相关生物基因组作图的参考点。本发明的蛋白质(在下文中也称为代谢途径蛋白质或MP蛋白质)具有表1/第7列中每一情况下所指定的功能。而且，它们在每一种情况下具有下列所述的氨基酸序列，所述氨基酸序列即在从表1/第2列每一情况下所提到的氨基酸序列开始的、对应于表1/第4列中对该氨基酸序列所指定的氨基酸位置中的至少一个位置上具有与表1/第5列同一行中所指定的特定氨基酸不同的生蛋白氨基酸。“相应”氨基酸位置优选指本发明的MP蛋白质氨基酸序列的氨基酸位置，其可由熟练技术人员通过将所述氨基酸序列与表1/第2列中每一情况下所提到的、具有表1/第4列中每一情况下对所述氨基酸序列所指定的氨基酸位置的氨基酸序列进行如下比较容易地找到a)同源比较，或b)二级结构、三级结构和/或四级结构的结构比较。比较氨基酸序列同源性的优选方法为例如通过使用Clustal法(HigginsDG，SharpPM.Fastandsensitivemultiplesequencealignmentsonamicrocomputer.ComputAppl.Biosci.1989年4月；5(2)151-1)的DNASTAR公司(Madison，Wisconsin，美国)的LasergeneSoftware软件并设定成下列参数进行多重比对参数空隙罚分10空隙长度罚分10双序列比对参数k-字(k-tuple)1空隙罚分3窗口5所保存Diagonals5在优选的实施方案中，蛋白质具有表1/第7列中各种情况下所指定的功能以及具有下列所述氨基酸序列，所述氨基酸序列即在从表1/第2列每一情况下所提到的氨基酸序列开始的、对应于表1/第4列中对该氨基酸序列所指定的氨基酸位置中的至少一个位置上具有与表1/第5列同一行中所指定的特定氨基酸不同的生蛋白氨基酸，其前提条件是根据表2的突变蛋白质除外。在进一步优选的实施方案中，本发明蛋白质具有表1/第2列中每一情况下所提到的氨基酸序列，其中所述蛋白质在表1/第4列中对该氨基酸序列所指定的氨基酸位置中的至少一个位置上具有与表1/第5列同一行中所指定的特定氨基酸不同的生蛋白氨基酸。在进一步优选的实施方案中，本发明蛋白质具有表1/第2列中每一情况下所提到的氨基酸序列，其中所述蛋白质在表1/第4列中对该氨基酸序列所指定的氨基酸位置之一具有与表1/第5列同一行中所指定的特定氨基酸不同的生蛋白氨基酸。表1/第2列中指定的氨基酸序列为谷氨酸棒杆菌野生型序列。表1/第4列指定了对于特定野生型氨基酸序列的至少一个氨基酸位置，其中本发明蛋白质的氨基酸序列具有与表1/第5列同一行指定的特定氨基酸不同的生蛋白氨基酸。在进一步优选的实施方案中，蛋白质在表1/第4列中对氨基酸序列所指定的氨基酸位置中的至少一个位置具有表1/第6列同一行所指定的氨基酸。本发明的另一个方面涉及分离的MP蛋白质或其部分，例如其生物活性部分。在优选实施方案中，分离的MP蛋白质或其部分在转录、翻译或翻译后调节生物、特别是棒杆菌和短杆菌(Brevibacterium)中的一个或多个代谢途径。可将MP多肽或其生物活性部分与非MP多肽进行功能连接以产生融合蛋白质。在优选实施方案中，该融合蛋白质的活性不同于单独MP蛋白质的活性，在其它优选实施方案中，所述融合蛋白质在转录、翻译或翻译后调节生物(特别是棒杆菌和短杆菌，优选谷氨酸棒杆菌)中的一个或多个代谢途径。在特别优选的实施方案中，所述融合蛋白质向宿主细胞的整合调节了细胞对目的化合物的生产。本发明进一步涉及编码上述本发明蛋白质的分离的核酸。这些核酸在下文也称为代谢途径核酸或MP核酸或MP基因。这些新的MP核酸分子编码本发明的MP蛋白质。这些MP蛋白质可以例如在对于细胞的正常代谢功能至关重要的蛋白质的转录、翻译或翻译后调节中发挥作用。由于用于谷氨酸棒杆菌的克隆载体(如描述于例如Sinskey等人，美国专利号4,649,119)以及用于谷氨酸棒杆菌及相关短杆菌物种(如乳糖发酵短杆菌(Brevibacteriumlactofermentum))的遗传操作技术(Yoshihama等人，J.Bacteriol.162(1985)591-597；Katsumata等人，J.Bacteriol.159(1984)306-311以及Santamaria等人，J.Gen.Microbiol.130(1984)2237-2246)的可得性，可将本发明的核酸分子用于该生物的遗传操作以便使其成为一种或多种精细化学品的更好和更有效的生产者。制备本发明核酸序列的适当起始点为例如谷氨酸棒杆菌菌株的基因组，其可从美国典型培养物保藏中心以名称ATCC13032获得。可使用习惯方法，使用表1所列的修饰从这些核酸序列制备本发明的核酸序列。对于由本发明MP蛋白质的氨基酸序列反翻译成的新的MP基因的核酸序列，有利地使用本发明的MP核酸将要导入并且本发明核酸所存在的生物中的密码子选择。例如，对于谷氨酸棒杆菌，有利地使用谷氨酸棒杆菌密码子选择。特定生物的密码子选择可以本身已知的方式从描述目的生物中至少一种蛋白质和编码该蛋白质的一个基因的数据库或专利公开中确定。通过向表1/第1列的核苷酸序列中引入一个或多个核苷酸替代、加入或缺失产生编码MP蛋白质的分离的核酸分子，以致于一个或多个氨基酸替代、加入或缺失被引入到所编码蛋白质中。通过标准技术例如位点特异性诱变和PCR介导诱变，将突变引入到表1/第1列中的序列之一。优选地，在一个或多个所预测非必需氨基酸残基处引入保守氨基酸替代。“保守氨基酸替代”为将氨基酸残基用具有类似侧链氨基酸残基进行取代的一种替代。具有类似侧链的氨基酸残基家族已在本领域中进行了定义。这些家族包括具有碱性侧链(如赖氨酸、精氨酸、组氨酸)、酸性侧链(如天冬氨酸、谷氨酸)、不带电荷极性侧链(如甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、非极性侧链(如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、β-分支侧链(如苏氨酸、缬氨酸、异亮氨酸)及芳族侧链(如酪氨酸、苯丙氨酸、色氨酸、组氨酸)的氨基酸。因此，在MP蛋白质中的预测非必需氨基酸残基优选由相同侧链家族的另一个氨基酸残基取代。备选地，在另一个实施方案中，通过例如饱和诱变，可以将沿着全部或部分MP编码序列随机引入突变，并且测试所得到突变体是否具有文中所述MP活性以便鉴定出保留MP活性的突变体。在附录A的序列之一诱变之后，可将所编码蛋白质重组表达，并且使用例如文中所述的测定法测定所述蛋白质的活性(见实施例部分的实施例8)。本发明基于生产可获得的新的分子，其中所述新分子在本文中称为MP核酸和MP蛋白质分子并且其通过转录、翻译或翻译后方式调节生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)中一个或多个代谢途径。在一个实施方案中，MP分子在转录、翻译或翻译后调节生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)中一个代谢途径。在优选实施方案中，本发明MP分子调节生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)中一个或多个代谢途径的活性对所述生物生产目的精细化学品具有作用。在特别优选的实施方案中，本发明MP分子具有调节活性，以致于生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)中的受本发明MP蛋白质调节的代谢途径在其效率或其生产量方面受到调节，并且这直接或间接调节了生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)生产目的精细化合品的生产量、生产和/或效率。术语“MP蛋白质”或“MP多肽”包含在转录、翻译或翻译后调节生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)中代谢途径的蛋白质。MP蛋白质的实例包括表1所列的那些。术语“MP基因”和“MP核酸序列”包括编码包含编码区和相应非翻译5’序列区和3’序列区的MP蛋白质的核酸序列。MP基因的实例列于表1。术语“产量”和“生产力”为本领域所知并且包括在预定时间间隔和预定发酵体积内所生产发酵产物(例如目的精细化学品)的浓度(例如每升每小时生产的产物千克数)。术语“生产效率”包含达到特定产物数量所需的时间(例如细胞达到生产精细化学品的特定生产率所需的时间)。术语“生产量”或“产物/碳生产量”为本领域所知并且包含转换碳源成为产物(即精细化学品)的效率。这通常表示为例如每千克碳源产生产物的千克数。增加化合物的生产量或生产增加了所获得分子的量或者增加了在特定培养体积中于预定阶段内此化合物的适宜获得分子的量。术语“生物合成”和“生物合成途径”为本领域所知并且包括通过细胞在例如多步骤过程中或高度调节的过程中从中间体合成化合物、优选有机化合物。术语“降解”及“降解途径”为本领域所知并且包含通过细胞在例如多步骤过程中或高度调节的过程中降化合物、优选有机化合物断裂成降解产物(在更概括的术语中为更小的或较不复杂的分子)。术语“代谢”为本领域所知并且包含发生于生物中的生化反应全体。因此，特定化合物的代谢(例如氨基酸如甘氨酸的代谢)包含细胞中此化合物的所有生物合成、修饰和降解途径。术语“调节”为本领域所知并且包含用以控制另一个蛋白质活性的蛋白质活性。术语“转录调节”为本领域所知并且包含用以抑制或活化编码靶标蛋白质的DNA转变成为mRNA的蛋白质活性。术语“翻译调节”为本领域所知并且包含用以抑制或活化编码靶标蛋白质的mRNA转变成为蛋白质分子的蛋白质活性。术语“翻译后调节”为本领域所知并且包含通过共价修饰该靶标蛋白质(如通过甲基化、糖基化或磷酸化)而用以抑制或改善靶标蛋白质活性的蛋白质活性。通过操作本发明的基因可直接或间接引起精细化学品生产量、生产和/或生产效率的提高。更加具体而言，对通常调节精细化学品代谢途径中精细化学品生产量、生产和/或生产效率的MP蛋白质的修饰可以对该生物生产一种或多种这些目的化合物的总产量或生产速率具有直接影响。对涉及这些代谢途径的那些蛋白质进行修饰也可以对目的精细化学品的生产量、生产和/或生产效率具有间接影响。代谢调节不可避免地具有复杂性并且影响不同途径的调节机制可能在许多地方重叠以致于根据特定细胞事件可快速地调整一个以上的代谢途径。这使得对一个代谢途径的调节蛋白质进行修饰还可以影响许多其它代谢途径，其中一些代谢途径可能涉及目的精细化学品的生物合成或降解。以此间接方式，对MP蛋白质作用的调节可以对经过与所述MP蛋白质直接调节不同的代谢途径生产精细化学品的生产具有影响。可使用本发明的MP核酸和MP蛋白质分子以便直接提高非人生物生产一种或多种目的精细化学品的生生产量、生产和/或生产效率。通过本领域已知的基因重组技术，能够操作本发明的一种或多种调节蛋白质以便使其功能能受调节。涉及抑制编码氨基酸生物合成所需酶的基因进行转录的MP蛋白质的突变使得该氨基酸不再能够抑制所述转录，其可以使该氨基酸的生产增加。因此，导致涉及目的精细化学品生物合成的MP蛋白质翻译增加或活化其翻译后修饰的MP蛋白质活性修饰，可以增加该化学品的生产。相反情况可能同样有用通过对涉及调节化合物降解途径的MP蛋白质的转录或翻译抑制的增加或者通过对其进行翻译后阴性修饰，可以增加该化合物的生产。在任何情况，可提高目的精细化学品的总生产量或生产速率。同样，本发明蛋白质和核苷酸分子中的所述修饰可以通过间接机制改善精细化学品的生产量、生产和/或生产效率。特定化合物的代谢不可避免地与细胞中的其它生物合成和降解途径相连接，并且一个代谢途径中的必需辅因子、中间体或底物可能通过另一个代谢途径提供或者被另一个代谢途径抑制。因此，对本发明的一种或多种调节蛋白质的调节会影响精细化学品的其它生物合成或降解途径的活性。除此以外，对一种或多种调节蛋白质进行操作可以提高细胞在培养中、特别是在亚优化的大规模发酵培养中生长和繁殖的总体能力。通过例如将通常作为对营养物的亚优化细胞外供应作出响应而抑制核苷生物合成(由此阻止细胞分裂)的本发明MP蛋白质进一步突变，以致于所述蛋白质具有较低的抑制活性，可以提高核苷酸的生物合成并且可能提高细胞分裂。导致培养中的细胞生长增加和细胞分裂增加的MP蛋白质中的修饰可以导致一种或多种目的精细化学品来自培养物中的生产量、生产和/或生产效率的增加，这至少是因为培养中的生产所述化学品的细胞数目的增加。本发明提供新的核酸分子，其编码能够实施涉及对非人生物中代谢途径进行转录、翻译或翻译后调节的酶步骤的蛋白质。编码MP蛋白质的核酸分子在本文中称为MP核酸分子。在优选实施方案中，MP蛋白质涉及对一个或多个代谢途径进行转录、翻译或翻译后调节。此种蛋白质的实例为由表1所列基因编码的那些蛋白质。因此，本发明的一个方面涉及分离的核酸分子(如cDNA)，其包含编码MP蛋白质或其生物活性部分的核苷酸序列以及适于作为检测或扩增MP编码核酸(如DNA或mRNA)的引物或杂交探针的核酸片段。在其它优选实施方案中，分离的核酸分子编码表1所列的任一个氨基酸序列。本发明优选的MP蛋白质同样优选具有本文中所描述MP活性中的至少一种。在另一个实施方案中，分离的核酸分子长度至少为15个核苷酸并且在严格条件下与本发明核酸分子杂交。分离的核酸分子优选对应于天然存在的核酸分子。分离的核酸更加优选编码天然存在的谷氨酸棒杆菌MP蛋白质或其生物活性部分。所有活细胞具有复杂分解代谢和合成代谢的能力，其中许多代谢途径彼此连接。为了保持该极端复杂代谢网络中不同部分间的平衡，细胞采用了细微调整的调节网络。通过独立或同时调节酶的合成和酶活性，细胞可调节完全不同代谢途径的活性以符合细胞的改变需要。酶合成的诱导或抑制可在转录或翻译水平或两种水平上发生(对于综述见Lewin，B.(1990)GeneIV，Part3“Controllingprokaryoticgenesbytranscription”，OxfordUniversityPress，Oxford，第213-301页，及其中的参考文献，Michal，G.(1990)BiochemicalPathwaysAnAtlasofBiochemistryandMolecularBiology，JohnWiley&Sons)。所有这些已知调节过程可以通过其自身对多种外部影响(例如温度、营养物供应或光)作出反应的另外的基因介导。涉及此类调节的蛋白质因子的实例包括转录因子。这些转录因子为结合至DNA而由此造成基因表达增加(如大肠杆菌(E.coli)ara操纵子情况下的正调节)或降低(如大肠杆菌lac操纵子情况下的负调节)的蛋白质。这些调节表达的转录因子本身可受到调节。其活性可通过例如结合至DNA-结合蛋白质的低分子量化合物调节，由此所述蛋白质与DNA上适当结合位置的结合受到刺激(如对于ara操纵子的阿拉伯糖的情况)或抑制(如对于lac操纵子的乳糖的情况)(见例如Helmann，J.D.及Chamberlin，M.J.(1988)“Structureandfunctionofbacterialsigmafactors”Ann.Rev.Biochem.57839-872；Adhya，S.(1995)“Thelacandgaloperonstoday”及Boos，W.等人，“Themaltosesystem”，两者皆在RegulationofGeneExpressioninEscherichiacoli(Lin，E.C.C.和Lynch，A.S.编者)Chapman&HallNewYork，第181-200页和第201-229页；以及Moran，C.P.(1993)“RNApolymeraseandtranscriptionfactors”在Bacillussubtillsandothergram-positvebacteria，Sonenshein，A.L.编者，ASMWashington，D.C.第653-667页)。蛋白质合成不仅在转录水平受到调节，也常常在翻译水平受到调节。这种调节可以通过多种机制实现，所述机制包括修饰核糖体结合一种或多种mRNA的能力、核糖体与mRNA的结合、保持或去除mRNA二级结构、使用对特定基因而言常用或较不常用的密码子、一种或多种tRNA的充足程度以及特异性调节机制例如衰减(见Vellanoweth，R.I.(1993)TranslationanditsregulationinBacillussubtillsandothergram-positivebacteria，Sonenshein，A.L.等人编者，ASMWashington，D.C.第699-711页，及其中的参考文献)。转录和翻译调节可针对单一蛋白质(连续调节)或同时针对多种代谢途径中的多个蛋白质(协调调节)进行。其表达受到协调方式调节的基因常常位于与操纵子或调节子紧密邻近的基因组中。基因转录和基因翻译的此种上调或下调受细胞内或细胞外多种因子的量控制，其中所述因子例如底物(在一个或多个代谢途径中使用的前体和中间体)、代谢物(通过与能够产生相关的生物化学代谢途径从复杂有机分子如糖降解产生的分子)和终产物(在代谢途径终结所得到的分子)。编码对于特定代谢途径活性所需的酶的基因表达可通过大量的该代谢途径底物分子而诱导。相应地，该基因表达通过途径终产物在细胞内的大量存在而抑制(Snyder，L.和Champness，W.(1977)TheMolecularBiologyofBacteriaASMWashington)。基因表达同样可受其它外部及内部因子调节，例如环境条件(如热、氧化应激或饥饿)。这些总体环境改变引起特异性调节基因表达的改变，其通过与DNA的结合并由此诱导或抑制转录而直接或间接(经由其它基因或蛋白质)引起基因表达(见例如Lin，E.C.C.和Lynch，A.S.编者，(1995)RegulationofGeneExpressioninEscherichiacoli，Chapman&HallNewYork)。可调节细胞代谢的另一个机制发生在蛋白质水平。该调节通过其它酶的活性或通过阻止或赋予蛋白质正常功能的低分子量成分的结合而实现。通过低分子量化合物结合进行蛋白质调节的实例包括GTP或NAD的结合。低分子量化学品的结合通常为可逆的，例如在GTP-结合蛋白质的例子中。这些蛋白质存在两种状态(结合GTP或GDP)，其中一个状态为蛋白质的活性形式而另一个为无活性形式。蛋白质活性受其它酶作用的调节，其通常是通过蛋白质的共价修饰(即氨基酸残基如组氨酸或天冬氨酸的磷酸化或甲基化)。此共价修饰通常为可逆的并且其通过具有相反活性的酶实现。该种情况的实例为激酶和磷酸化酶在蛋白质磷酸化中的相反活性蛋白质激酶磷酸化靶标蛋白质上的特异性残基(如丝氨酸或苏氨酸)，而蛋白质磷酸化酶从该蛋白质去除磷酸基。调节其它蛋白质活性的酶通常通过外部刺激进行自身调节。这些刺激通过作为传感器的蛋白质进行介导。通过这些传感器蛋白质介导该外部信号的众所周知的机制为二聚化，但也知道其它的机制(见例如Msadek，T.等人，(1993)“Two-componentRegulatory-Systems”在BacilliussubtillsandOtherGram-PositiveBacteria，Sonenshein，A.L.等人，编者，ASMWashington，第729-745页，及其中参考文献)。详细了解控制微生物中细胞代谢的调节网络对于通过发酵产生高产量化学品极为重要。为了提高目的化学品的合成可将下调代谢途径的控制系统去除或降低，并且相应地，可将目的产物代谢途径上调的那些组成型活化或对于活性进行优化(如Hirose，Y.和Okada，H.(1979)“MicrobialProductionofAminoAcids”在Peppler，H.J.和Perlman，D.(编者)MicrobialTechnology，第2版，第1卷，第7章，AcademicPress，NewYork中所示)。本发明的另一个方面涉及核酸构建体，例如载体如重组表达载体，例如含有至少一种本发明核酸的重组表达载体。该核酸构建体优选以功能性连接方式包含启动子以及(如果适当情况下)终止子。特别优选的是给出对于核酸而言为异源的并且能够在非人生物中表达所述核酸的启动子。在优选的棒杆菌或短杆菌属生物中特别优选的启动子实例为tac启动子。本发明进一步涉及通过转化非人母体生物而制备经基因修饰的非人生物的方法，其中所述转化是向所述母体生物中导入a)至少一种上述本发明MP核酸，或b)至少一种上述本发明核酸构建体，或c)对于上述本发明的内源MP核酸而言为外源的并且能够使本发明的该内源MP核酸在该生物中表达的启动子。优选地，将根据实施方案c)的启动子被导入生物中以致于使启动子在该生物中与本发明的内源MP核酸功能性连接。“功能性连接”意思是指一种功能性的连接，即能够使本发明的内源MP核酸通过所导入的启动子进行表达的连接。术语“母体生物”意思是指与转化成为经基因修饰生物相对应的非人生物。母体生物在此可为野生型生物或已进行基因修饰的生物。此外，母体生物可以是已经能够生产目的精细化学品或者通过本发明的转化而能够生产目的精细化学品。术语“经基因修饰的生物”优选指与母体生物相比进行了基因修饰的生物。视上下文而定，术语“生物”意思是指非人母体生物或本发明的经基因修饰的非人生物或两者。可将本发明的MP核酸或本发明的核酸构建体以染色体形式或以作为自主复制质粒的质粒形式导入。优选将本发明的MP核酸或本发明的核酸构建体整合入染色体。在优选实施方案中，所使用母体生物为已经能够生产目的精细化学品的生物。在特别优选的棒杆菌属细菌生物和特别优选的精细化学品赖氨酸、甲硫氨酸及苏氨酸当中，特别优选已经能够生产赖氨酸的那些母体生物。特别优选的是其中例如编码天冬氨酸激酶的基因(ask基因)被解除调节或者反馈抑制已被去除或降低的棒杆菌。例如，这些类型的细菌在ask基因中具有造成反馈抑制的降低或去除的突变，例如突变T311I。因此本发明具体而言涉及通过上述方法可得到的经基因修饰的生物。此外本发明还涉及用下列转化的经基因修饰的非人生物a)至少一种上述本发明MP核酸，或b)至少一种上述本发明核酸构建体，或c)对于上述本发明的内源MP核酸而言为外源的并且能够使本发明的该内源MP核酸在该生物中表达的启动子。在另一个实施方案中，母体生物基因组中的内源MP基因已经通过与所修饰MP基因的同源重组而进行了修饰，例如在功能上被破坏。优选地，本发明核酸的表达导致与母体生物相比该生物的精细化学品生产受到调节。优选非人生物为植物、藻类和微生物。优选微生物为细菌、酵母菌或霉菌。特别优选的微生物为细菌，特别是棒杆菌属或短杆菌属细菌，特别优选的是谷氨酸棒杆菌。作为母体生物或生物或经基因修饰生物的特别优选的棒杆菌属或短杆菌属细菌为下表3中所列的细菌。表3缩写具有下列意义ATCC美国典型培养物保藏中心(AmericanTypeCultureCollection，Rockville，MD，美国)FERM发酵研究所(FermentationResearchInstitute，Chiba，日本)NRRL农业研究机构保藏中心(ARSCultureCollection，NorthernRegionalResearchLaboratory，Peoria，IL，美国)CECT西班牙典型培养物保藏中心(ColeccionEspanoladeCultivosTipo，Valencia，西班牙)NCIMB国立工业和海洋微生物保藏有限公司(NationalCollectionofIndustrialandMarineBacteriaLtd.，Aberdeen，英国)CBS真菌菌种保藏中心(CentraalbureauvoorSchimmelcultures，Baarn，荷兰)NCTC国立典型培养物保藏中心(NationalCollectionofTypeCultures，London，英国)DSMZ德国微生物保藏和细胞培养中心(DeutscheSammlungvonMikroorganismenundZellkulturen，Brunswick，德国)另一个优选实施方案为本发明的经基因修饰生物(在下文中也称为“宿主细胞”)，其具有一个以上的本发明MP核酸分子。此宿主细胞可以技术人员已知的多种方式制备。例如，通过携带数个本发明核酸分子的载体将其转染。然而，也可以使用在每一情况下将本发明的一个核酸分子导入宿主细胞的载体，并且因此同时或连续使用多种载体。因此可以构建携带众多(多达数百个)本发明核酸序列的细胞。此种累积对宿主细胞在精细化学品生产力方面常常产生超加性效果。在优选实施方案中，经基因修饰的生物以染色体整合方式包含至少两种本发明MP核酸或者与本发明内源MP核酸功能性连接的异源启动子。在另一个实施方案中，本发明的MP蛋白质和/或MP基因能够调节生物(特别是棒杆菌或短杆菌，特别优选谷氨酸棒杆菌)中目的精细化学品的生产。借助于基因重组技术，可以操作一个或多个发明的代谢途径调节蛋白质，以致于其功能受到调节。例如，可以改进生物合成酶的效率或者破坏其变构控制区域以致于阻止化合物生产的反馈抑制。因此，可将降解酶删除或通过替代、缺失或添加进行修饰使得其对目的化合物的降解活性降低，而不损害细胞生存力。在任何情况下，可以提高任何所述目的精细化学品的总体生产量或生产速率。本发明蛋白质和核苷酸分子中的这些修饰还可以间接改进精细化学品的生产。细胞中代谢途径的调节机制不可避免地发生连接，并且一个代谢途径的活化可以以伴随方式导致另一个代谢途径的抑制或活化。调节本发明一个或多个蛋白质的活性可影响其它精细化学品生物合成或降解途径的生产或效率。降低MP蛋白质抑制编码氨基酸生物合成中特定蛋白质的基因转录的能力使得可以同时抑制其它氨基酸生物合成途径，因为这些代谢途径彼此相连。通过修饰本发明的MP蛋白质，能够在一定程度上将细胞生长和细胞分裂与其细胞外环境解偶联；对于细胞生长和细胞分裂的细胞外条件为亚优化时MP蛋白质通常抑制核苷酸的生物合成，当通过影响MP蛋白质使其缺乏此功能时，则可以使其生长，即使细胞外条件不佳时也可生长。这对于大规模发酵培养特别重要，因为对于温度、营养供应或通气方面，此种培养条件常常为亚优化的，但在去除该因子的细胞调节系统之后该培养条件仍能促进生长及细胞分裂。因此，本发明还涉及通过培养上述本发明的经基因修饰生物而制备精细化学品的方法。此外，本发明涉及制备精细化学品的方法，其通过下属步骤进行A)用下列转化非人母体生物a)至少一种上述本发明MP核酸，或b)至少一种上述本发明核酸构建体，或c)对于上述本发明的内源MP核酸而言为外源的并且能够使本发明的该内源MP核酸在该生物中表达的启动子，和B)培养根据A)项制备的经基因修饰生物。将经基因修饰的生物用本身已知的方式并按照生物进行培养。例如，将细菌培养于适当发酵介质中的液体培养物中。在优选实施方案中，将至少一种精细化学品从经基因修饰生物和/或培养步骤后的培养基分离。术语“精细化学品”为本领域所知并且包括由生物生产并用于许多种工业例如(但不限于)制药业、农业、化妆品业、食品业及饲料业的化合物。这些化合物包含有机酸，例如酒石酸、衣康酸及二氨基庚二酸、生蛋白氨基酸及非生蛋白氨基酸、嘌呤及嘧啶碱基、核苷及核苷酸(如描述于例如Kuninaka，A.(1996)Nucleotidesandrelatedcompounds，第561-612页，在Biotechnology，第6卷，编者Rehm等，VCHWeinheim及其中的参考文献)、脂类、饱和及不饱和脂肪酸(例如花生四烯酸)、二元醇(如丙二醇及丁二醇)、碳水化合物(如透明质酸及海藻糖)、芳族化合物(如芳族胺、香草醛及靛蓝)、维生素及辅因子(如描述于Ullmann’sEncyclopediaofIndustrialChemistry，第A27卷，“Vitamins”，第443-613页，(1996)VCHWeinheim及其中的参考文献；及Ong，A.S.，Niki，E.和Packer，L.(1995)“Nutrition，Lipids，HealthandDisease”UNESCO会议录/ConfederationofScientificandTechnologicaiAssociationsinMalaysia和theSocietyforFreeRadicalResearch-Asia，1994年9月1-3日于马来西亚槟城举行，AOCSPress(1995))、酶及由Gutcho(1983)在ChemicalsbyFermentation，NoyesDataCorporation，ISBN0818805086及其中指出的参考文献中所述的所有其它化学品。以下进一步说明特定精细化学品的代谢及用途。I.氨基酸的代谢和用途氨基酸构成所有蛋白质的基础结构单元并且因此对于细胞的正常功能是必需的。术语“氨基酸”为本领域所知。生蛋白氨基酸有20种，它们作为蛋白质的结构单元起作用，在蛋白质中氨基酸通过肽键连接在一起，而非生蛋白氨基酸(已知有数百种)通常不出现在蛋白质中(见Ullmann’sEncyclopediaofIndustrialChemistry，第A2卷，第57-97页，VCHWeinheim(1985))。虽然L-氨基酸是天然存在蛋白质中常见的唯一一种类型，但是氨基酸可以以D或L构型存在。20种生蛋白氨基酸中每一种的生物合成和降解途径已经在原核细胞和真核细胞中进行了很好表征(见例如，Stryer，L.Biochemistry，第3版，第578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)可通过简单的生物合成途径转变成其它11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)，对于“必需”氨基酸，之所以如此称呼是因为由于其生物合成的复杂性其必须从食物中摄取。高等动物能够合成这些氨基酸中的一些氨基酸，但是必须从食物中摄取必需氨基酸以便进行正常蛋白质合成。除了其在蛋白质生物合成中的功能以外，这些氨基酸也是令人感兴趣的化合物，例如已经发现许多氨基酸在食品、动物饲料、化学品、化妆品、农业及制药业中具有多种应用。不但对于人类营养，而且对于单胃家畜如家禽及猪，赖氨酸为重要的氨基酸。谷氨酸为最常用作调味添加剂(谷氨酸单钠，MSG)，并且也可用于其它食品业，天冬氨酸、苯丙氨酸、甘氨酸及半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸皆用于制药业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸及丙氨酸用于制药业及化妆品业。苏氨酸、色氨酸及D/L-甲硫氨酸广泛使用于动物饲料添加剂(Leuchtenberger，W.(1996)Aminoacids-Technicalproductionanduse，第466-502页，在Rehm等人，(编者)Biotechnology，第6卷，第14a章，VCHWeinheim)。已经发现，这些氨基酸额外适合作为合成氨基酸及蛋白质合成的前体，如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟基色氨酸以及描述于Ullmann’sEncyclopediaofIndustrialChemistry，第A2卷，第57-97页，VCHWeinheim，1985中的其它物质。已经很好地表征了这些天然氨基酸在能够生产它们的生物(例如细菌)中的生物合成(对于细菌氨基酸生物合成及其调节的综述，见Umbarger，H.E.(1978)Ann.Rev.Biochem.47533-606)。谷氨酸通过α-酮戊二酸(柠檬酸循环中的一种中间产物)的还原性胺化反应合成。谷氨酰胺、脯氨酸和精氨酸的每一个由谷氨酸相继产生。丝氨酸的生物合成为三步骤反应过程，开始于3-磷酸甘油酸(糖酵解的中间产物)，并在氧化、转氨基和水解步骤之后得到此氨基酸。半胱氨酸和甘氨酸各自从丝氨酸产生，具体而言，前者通过高半胱氨酸与丝氨酸的缩合作用产生，而后者通过在丝氨酸转羟甲基酶催化的反应中将侧链β-碳原子转移至四氢叶酸而产生。苯丙氨酸和酪氨酸从糖酵解及戊糖磷酸途径的前体赤藓糖-4-磷酸及磷酸烯醇丙酮酸于9步骤生物合成途径中合成，其仅在预苯酸合成后的最后两步中分开。色氨酸同样从这两个起始分子产生，但其通过11步的途径合成。酪氨酸也可通过苯丙氨酸羟化酶催化反应从苯丙氨酸制备。丙氨酸、缬氨酸和亮氨酸各自从糖酵解终产物丙酮酸生物合成。天冬氨酸从草酰乙酸(柠檬酸循环的中间产物)形成。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸各自通过天冬氨酸转化产生。异亮氨酸从苏氨酸形成。组氨酸在复杂的9步途径中从5-磷酸核糖-1-焦磷酸(一种活化糖)形成。超过细胞蛋白质生物合成所需的氨基酸量无法被储存，而是被分解，所以给细胞中的主要代谢途径提供中间产物(对于综述，见Stryer，L.Biochemistry，第3版，第21章，“AminoAcidDegradationandtheUreaCycle”，第495-516页，(1988))。虽然细胞能够将不需要的氨基酸转变成为有用的代谢中间产物，但是就合成所需的能量、前体分子及酶而言氨基酸的生产是昂贵。因此毫不奇怪氨基酸生物合成受反馈抑制的调节，由此特定氨基酸的存在使其自身生产减慢或完全停止(对于氨基酸生物合成途径中反馈机制的综述，见Stryer，L.Biochemistry，第3版，第24章，“BiosynthesisofAminoAcidsandHeme”，第575-600页，(1988))。因此特定氨基酸的产出受细胞中该氨基酸量的限制。II.维生素、辅因子及营养补充品(Nutraceutical)的代谢和用途维生素、辅因子及营养补充品包含另一组分子。高等动物已丧失合成它们的能力，所以必须摄取，但是其可轻易地由其它生物如细菌合成。这些分子为本身具有生物活性分子或者为作为许多代谢途径中的电子载体或中间产物的生物活性物质前体。这些化合物除了具有营养价值以外，它们还作为着色剂、抗氧化剂和催化剂或其它加工辅料具有明显的工业价值。(对于这些化合物的结构、活性及工业应用的综述，见例如Ullmann’sEncyclopediaofIndustrialChemistry，“Vitamins”，第A27卷，第443-613，VCHWeinheim，1996)。术语“维生素”为本领域所知并且包含生物正常功能所需要但无法由该生物本身合成的营养物。维生素组可包括辅因子和营养补充品化合物。术语“辅因子”包含正常酶活性所需的非蛋白质化合物。这些化合物可以是有机的或无机的；本发明的辅因子分子优选是有机的。术语“营养补充品”包含在植物和动物、特别是人中具有健康促进作用的食物添加剂。此类分子的实例为维生素、抗氧化剂以及某些脂类(如多不饱和脂肪酸)。这些分子在能够生产它们的生物(如细菌)中的生物合成已被广泛地表征(Ullmann’sEncyclopediaofIndustrialChemistry，“Vitamins”，第A27卷，第443-613页，VCHWeinheim，1996，Michal，G(1999)BiochemicalPathwaysAnAtlasofBiochemistryandMolecularBiology，JohnWilely&Sons；Ong，A.S.，Niki，E.和Packer，L.(1995)“Nutrition，Lipids，HealthandDisease”UNESCO会议录/ConfederationofScientificandTechnologicalAssociationsinMalaysia和theSocietyforFreeRadicalResearch-Asia，1994年9月1-3日于马来西亚槟城举行，AOCSPress，Champaign，ILX，374S)。硫胺(维生素B1)由嘧啶及噻唑单元化学偶联形成。核黄素(维生素B2)从鸟苷5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素用于合成黄素单核酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。此化合物家族统称为“维生素B6”(例如吡哆醇、吡哆胺、吡哆醛5’-磷酸和商业使用的盐酸吡哆醇)，其皆为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸，R-(+)-N-(2，4-二羟基-3，3-二甲基-1-氧丁基)-β-丙氨酸)可通过化学合成或发酵制备。泛酸生物合成的最后步骤为ATP驱动的β-丙氨酸与泛解酸的缩合。负责转变成泛解酸和转变成β-丙氨酸以及缩合成泛酸的生物合成步骤的酶是已知的。泛酸的代谢活性形式为辅酶A，其生物合成由5个酶促步骤产生。泛酸、磷酸吡哆醛5’-磷酸、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸的形成，还催化(R)-泛解酸、(R)-泛解酸内酯、(R)-泛醇(维生素原B5)、泛酰巯基乙胺(及其衍生物)和辅酶A的生产。已经详细研究了微生物中生物素从前体分子庚二酰-CoA的生物合成，并且已经鉴定出几个有关基因。已显示许多相应蛋白质参与Fe簇合物合成并且属于nitS蛋白质类。硫辛酸源自辛酸并且作为能量代谢中的辅酶，它是丙酮酸脱氢酶复合物和α-酮戊二酸脱氢酶复合物的组成成分。叶酸类为全部源自叶酸的一组物质，叶酸依次从L-谷氨酸、对氨基苯甲酸和6-甲基喋呤衍生而来。已经在某些微生物中详细研究了从代谢中间产物鸟苷5’-三磷酸(GTP)、L-谷氨酸和对氨基苯甲酸开始的叶酸及其衍生物的生物合成。类咕啉(如钴胺素，特别是维生素B12)和卟啉属于通过四吡咯环系统区别的一组化学品。维生素B12的生物合成太过复杂以至于尚未完全了解，但现已知许多有关的酶及底物。烟酸(烟酸酯)及烟酰胺为吡啶衍生物，也称为“尼克酸”。尼克酸是重要辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)及其还原形式的前体。虽然这些化合物中有一些已经通过大规模微生物培养而生产，如核黄素、维生素B6、泛酸和生物素，但是这些化合物在工业规模上的生产主要基于无细胞的化学合成。只有维生素B12由于其合成的复杂性而只能通过发酵生产。体外方法需要相当大的材料花费和时间，并且成本常常很高。III.嘌呤、嘧啶、核苷及核苷酸的代谢和用途用于嘌呤和嘧啶代谢的基因及其相应蛋白质对于肿瘤和病毒感染的治疗为重要的目标。术语“嘌呤”或“嘧啶”包含含氮碱基，其形成核酸、辅酶及核苷酸的部分。术语“核苷酸”包含核酸分子的基础结构单元，其包含含氮碱基、戊糖(在RNA的情况中此糖为核糖，在DNA的情况中此糖为D-脱氧核糖)和磷酸。术语“核苷”包含作为核苷酸前体的分子，但与核苷酸相比其无磷酸单元。通过抑制这些分子的生物合成或者通过抑制其动员形成核酸分子，可以抑制RNA和DNA合成；在癌细胞中对该活性的靶向抑制则能够抑制肿瘤细胞分裂和复制的能力。还有一些核苷酸不形成核酸分子而是作为能量储存(即AMP)或辅酶(即FAD和NAD)。几个出版物已经描述了这些化学品在医学指征中的用途，其中嘌呤和/或嘧啶代谢受到影响(例如Christophrson，R.I.和Lyons，S.D.(1990)，“Potentinhibitorsofdenovopyrimidineandpurinebiosynthesisaschemotherapeuticagents”，Med.Res.Reviews10505-548)。对嘌呤和嘧啶代谢所涉及酶的研究集中于可以用作例如免疫抑制剂或抗增殖剂的新药开发上(Smith，J.L.“EnzymesinNucleotideSynthesis”Curr.Opin.Struct.Biol.5(1995)752-757；Simmonds，H.A.，Biochem.Soc.Transact.23(1995)877-902)。然而，嘌呤及嘧啶碱基、核苷及核苷酸还有其它可能的用途作为多种精细化学品生物合成中的中间产物(如硫胺、S-腺苷甲硫氨酸、叶酸或核黄素)、作为细胞的能量载体(例如ATP或GTP)并且对于化学品自身通常用作增味剂(例如IMP或GMP)或用于许多医学应用(见例如Kuninaka，A.，(1996)“NucleotidesandRelatedCompoundsinBiotechnology”，第6卷，编者Rehm等，VCHWeinheim，第561-612页)。参与嘌呤、嘧啶、核苷或核苷酸代谢的酶也日渐成为为了作物保护而正在开发的化学品(包括杀真菌剂、除草剂和杀虫剂)所针对的目标。这些化合物在细菌中的代谢已被表征(对于综述，见例如Zalkin，H.和Dixon，J.E.(1992)“Denovopurinenucleotidebiosynthesis”，在ProgressinNucleicAcidsResearchandMolecularbiology，第42卷，AcademicPress，第259-287页；以及Michal，G.(1999)，“NucleotidesandNucleosides”；第8章，在BiochemicalPathwaysAnAtlasofBiochemistryandMolecularBiology，Wiley，NewYork)。嘌呤代谢为深入研究的目标，其为细胞正常功能所必需的。高等动物中嘌呤代谢紊乱可能引起严重的疾病，例如痛风。通过许多步骤从核糖5-磷酸经由中间体化合物肌苷5’-磷酸(IMP)合成嘌呤核苷酸，导致产生鸟苷5’-单磷酸(GMP)或腺苷5’-单磷酸(AMP)，由此可轻易地制备作为核苷酸使用的三磷酸形式。将这些化合物还可以作为能量储存使用，以致于它们的分解能够为细胞中的许多不同生物化学过程提供能量。嘧啶生物合成从核糖5-磷酸经由尿苷5’-单磷酸(UMP)进行。之后将UMP转变成胞苷5’-三磷酸(CTP)。所有核苷酸的脱氧形式都是由核苷酸的二磷酸核糖形式在一步还原反应中制备从而产生核苷酸的二磷酸脱氧核糖形式。磷酸化后，这些分子可参与DNA合成。IV.海藻糖代谢和用途海藻糖由以α，α-1，1键连接在一起的两个葡萄糖分子组成。它通常在食品业中用作甜味剂、干燥或冷冻食品及饮料中的添加剂。然而，其还用于制药业或化妆品业和生物工程业(见例如Nishimoto等人，(1998)美国专利号5759610；Singer，M.A.和Lindquist，S.TrendsBiotech.16(1998)460-467；Paiva，C.L.A和Panek，A.D.BiotechAnn.Rev.2(1996)293-314；以及Shiosaka，M.J.Japan172(1997)97-102)。海藻糖由许多微生物的酶生产并且被天然释放入周围培养基，可通过本领域已知的方法从所述培养基中分离海藻糖。特别优选的精细化学品是氨基酸，特别是选自L-赖氨酸、L-苏氨酸和L-甲硫氨酸的氨基酸。本发明的另一个方面涉及调节非人生物生产精细化学品的方法。这些方法包括将细胞与调节MP蛋白质活性或MP核酸表达的物质相接触，以致于与缺乏该物质时的同一活性相比细胞相关的活性受到修饰。在优选实施方案中，在生物(特别是棒杆菌和/或短杆菌属细菌，特别是谷氨酸棒杆菌)代谢途径的一个或多个调节系统方面将细胞进行调节，使得该宿主细胞生产目的精细化学品的生产量得到改善或者生产速率得到改善。调节MP蛋白质活性的物质刺激例如MP蛋白质活性或MP核酸表达。刺激MP蛋白质活性或MP核酸表达的物质的实例包括小分子、活性MP蛋白质和编码MP蛋白质并已被导入细胞内的核酸。抑制MP活性或MP表达的物质的实例包括小分子和反义MP核酸分子。本发明的另一个方面涉及调节细胞生产目的化合物的生产量的方法，其包括将MP基因到入细胞，其中基因或者在单独的质粒上维持或者整合进入宿主细胞基因组。整合进入基因组可以随机进行或者通过同源重组进行，导致细胞对目的化合物的生产得到调节。在优选实施方案中，生产量提高。在另一个优选实施方案中，精细化学品为氨基酸。在特别优选的实施方案中，该氨基酸为L-赖氨酸、L-甲硫氨酸或L-苏氨酸。下面的分部分更加详细描述本发明的多种方面和优选实施方案A.核酸分子的分离如本文中所使用，术语“核酸分子”旨在包含DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)并且还包括通过核苷酸类似物方式产生的DNA或RNA类似物。此外，此术语包含位于基因编码区3’端和5’端的非翻译序列编码区5’端上游的至少约100个核苷酸的序列和编码区3’端下游的至少约20个核苷酸的序列。核酸分子可以为单链或双链，但优选为双链DNA。“分离的”核酸分子是去除了在核酸天然来源中存在的其它核酸分子的核酸分子。“分离的”核酸优选不具有天然位于核酸所来源的生物基因组DNA两侧的任何序列(例如位于核酸5’端或3’端的序列)。在多种实施方案中，分离的MP核酸分子具有例如少于约5kb、4kb、3kb、2kb、1kb、0.5kb或0.1kb的、天然位于核酸所来源的细胞(如谷氨酸棒杆菌细胞)基因组DNA核酸分子两侧的核苷酸序列。除此之外，“分离的”核酸分子如cDNA分子基本上没有其它细胞材料或培养基(如果其是通过重组技术制备的)或者没有化学前体或其它化学品(如果其是化学合成的)。此外，可以通过聚合酶链式反应使用基于该序列产生的寡核苷酸引物分离核酸分子(例如，通过聚合酶链式反应使用已经基于附录A的完整序列产生的寡核苷酸引物可以分离包含附录A同一序列的核酸分子)。例如，可从正常内皮细胞分离mRNA(例如通过Chirgwin等人，(1979)Biochemistry185294-5299的异硫氰酸胍提取法)并且可以依靠反转录酶(如Moloney-MLV反转录酶，可从Gibco/BRL，Bethesda，MD获得，或AMV反转录酶，可从SeikagakuAmerica，Inc.，St.Petersburg，FL获得)制备cDNA。用于通过聚合酶链式反应扩增的合成寡核苷酸引物基于附录A所示任何核苷酸序列产生。按照标准PCR扩增技术，使用cDNA或者备选的基因组DNA作为模板扩增本发明的核酸。将以此方式扩增的核酸克隆入适当载体并且通过DNA序列分析进行表征。对应于MP核苷酸序列的寡核苷酸可利用例如自动DNA合成仪通过标准合成法制备。在优选实施方案中，本发明的分离的核酸分子包含表1/第1列所列核苷酸序列，其中所述核苷酸序列含有对应于表1/第4列的氨基酸位置的反翻译突变。在进一步优选的实施方案中，本发明的分离的核酸分子包含与任意上述核苷酸序列互补的核酸分子或其部分，所述核酸分子与任意上述核苷酸序列充分互补以致于其能够与任意上述核苷酸序列杂交产生稳定双链体。由本发明MP核酸分子所编码的蛋白质片段优选为任何MP蛋白质的生物活性片段。如本文中所使用，术语“MP蛋白质的生物活性片段”旨在包含MP蛋白质的片段例如结构域或基序，其可以在转录、翻译或翻译后水平调节谷氨酸棒杆菌的代谢途径或者具有表1所指出的活性。为了确定MP蛋白质或其生物活性片段是否可以在转录、翻译或翻译后水平调节谷氨酸棒杆菌的代谢途径，开展了酶活性分析。如实施例部分的实施例8中所详细描述的这些分析方法为熟练技术人员所熟悉。除了在种群中存在的其它天然存在MP序列变体以外，熟练技术人员还了解，可以通过进一步的突变将更多的改变引入表1中的核苷酸序列中，这导致与野生型相比所编码MP蛋白质的氨基酸序列具有更多的改变而不损害MP蛋白质的功能性。因此，可以制备例如表1序列中的核苷酸替代，其导致在“非必需”氨基酸残基处产生氨基酸替代。可将任何MP蛋白质(表1)野生型序列中的“非必需”氨基酸残基进行修饰而不改变该MP蛋白质的活性，而“必需”氨基酸残基为MP蛋白质活性所必需。然而，其它氨基酸残基(例如在具有MP活性的结构域中的非保守或仅半保守氨基酸残基)对于活性可能是非必需的并且因此可以进行修饰而不改变MP活性。B.重组表达载体和宿主细胞本发明的另一个方面涉及含有编码MP蛋白质的本发明核酸的核酸构建体，例如载体，优选表达载体。如本文中所使用，术语“载体”涉及能够转运其所结合的另一核酸的核酸分子。一种类型的载体为“质粒”，此术语意思是指另外的DNA片段连接其中的环状双链DNA环。另一类型载体为病毒载体，其允许另外的DNA片段连接入病毒基因组。一些载体能够在其所被导入的宿主细胞中自主复制(例如具有细菌复制起点的细菌载体和附加型哺乳动物载体)。当其它载体(如非附加型哺乳动物载体)被导入宿主细胞时，其被整合进入该宿主细胞的基因组，并且因此与宿主基因组一起复制。此外，特定载体能够控制其功能性连接基因的表达。这些载体称为“表达载体”。通常，用于DNA重组技术中的表达载体为质粒的形式。在本说明书中，“质粒”和“载体”可互换使用，因为质粒是最常使用的载体类型。本发明旨在包含发挥类似功能的所述其它类型表达载体，如病毒载体(例如复制缺陷型逆转录病毒、腺病毒及腺相关病毒)。本发明的重组表达载体以适合在宿主细胞表达该核酸的形式包含有本发明的核酸，这意味着重组表达载体包含一个或多个调节序列，其中所述调节序列基于用于表达的宿主细胞进行选择的并且其与待表达核酸序列功能性连接。在重组表达载体中，术语“功能性连接”意思是指目的核苷酸序列与调节序列如此连接以致于使该核苷酸序列的表达成为可能(例如在体外转录/翻译系统中或宿主细胞中(如果载体已被导入该宿主细胞中))。术语“调节序列”旨在包含启动子、增强子及其它表达控制元件(如聚腺苷酸化信号)。这些调节序列描述于例如GoeddelGeneExpressionTechnologyMethodsinEnzymology185，AcademicPress，SanDiego，CA(1990)。调节序列包含控制核苷酸序列在许多类型宿主细胞中组成型表达的那些调节序列以及控制该核苷酸序列仅在特定宿主细胞中直接表达的那些调节序列。熟练技术人员知道对表达载体的设计会取决于多种因素，例如对待转化宿主细胞的选择、目的蛋白质的表达程度等。本发明的表达载体可导入宿主细胞以便制备由文中所述核酸编码的蛋白质或肽，包括融合蛋白质或融合肽(例如MP蛋白质、突变形式的MP蛋白质、融合蛋白质等)。可将本发明的重组表达载体设计来在原核或真核细胞中表达MP蛋白质。例如，可将MP基因在细菌细胞如谷氨酸棒杆菌、昆虫细胞(利用杆状病毒表达载体)、酵母细胞和其它真菌细胞(见Romanos，M.A.等人，(1992)，“Foreigngeneexpressioninyeastareview”，Yeast8423-488；vandenHondel，C.A.M.J.J.等人，(1991)，“Heterologousgeneexpressioninfilamentousfungi”在MoreGeneManipulationsinFungi，编者J.W.Bennet和L.L.Lasure，第396-428页AcademicPressSanDiego；以及vandenHondel，C.A.M.J.J.和Punt，P.J.(1991)，“Genetransfersystemsandvectordevelopmentforfilamentousfungi”，在AppliedMolecularGeneticsofFungi，编者Peberdy，J.F.等人，第1-28页，CambridgeUniversityPressCambridge)、藻类及多细胞植物细胞(见Schmidt，R.和Willmitzer，L.(1988)，“HighefficiencyAgrobacteriumtumefaciens-mediatedtransformationofArabidopsisthalianaleafandcotyledonexplants”，PlantCellRep.583-586)或哺乳动物细胞中表达。适宜的宿主细胞进一步讨论于Goeddel，GeneExpressionTechnologyMethodsinEnzymology185，AcademicPress，SanDiego，CA(1990)。备选地，可通过例如使用T7启动子调节序列和T7聚合酶使重组表达载体体外转录和翻译。主要通过使用包含控制融合或非融合蛋白质表达的组成型或诱导型启动子的载体将蛋白质在原核生物中表达。。融合载体向其所编码的蛋白质中添加了许多氨基酸，通常在重组蛋白质的氨基末端添加。这些融合载体通常具有三个任务1)增强重组蛋白质的表达；2)增加重组蛋白质的溶解度；和3)通过作为亲和纯化中的配体支持重组蛋白质的纯化。常常将蛋白质水解位点引入融合表达载体的融合单元与重组蛋白质的连接处，以致于在融合蛋白质纯化之后可将重组蛋白质与融合单元分开。这些酶及其相应的识别序列包含Xa因子、凝血酶的肠激酶。常见融合表达载体包含pGEX(PharmaciaBiotechInc；Smith，D.B.和Johnson，K.S.(1988)Gene6731-40)、pMAL(NewEnglandBiolabs，Beverly，MA)和pRIT5(Pharmacia，Piscataway，NJ)，其中分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白质A与重组靶蛋白质融合。在一个实施方案中，将MP蛋白质的编码序列克隆入pGEX表达载体，以致于产生载体编码融合蛋白质的载体，其中所述融合蛋白质从N端至C端包含GST-凝血酶断裂位点-蛋白质X。通过亲和层析法使用谷胱甘肽-琼脂糖树脂将融合蛋白质纯化。通过用凝血酶裂解融合蛋白质得到不与GST融合的重组MP蛋白质。适宜可诱导非融合的大肠杆菌表达载体的实例包括pTrc(Amann等人，(1988)Gene69301-315)和pET11d(Studier等人，GeneExpressionTechnologyMethodsinEnzymology185，AcademicPress，SanDiego，California(1990)60-89)。靶基因从pTrc载体中的表达基于通过宿主RNA聚合酶从杂合trp-lac融合启动子进行的转录。靶基因从pET11d载体的表达基于从T7-gn10-lac融合启动子进行的转录，其由共表达的病毒RNA聚合酶(T7gn1)介导。该病毒聚合酶由BL21(DE3)或HMS174(DE3)宿主株中存在的λ原噬菌体提供，所述λ原噬菌体具有处于lacUV5启动子转录控制下的T7gn1基因。使重组蛋白质表达最大化的一个策略是在水解断裂所述重组蛋白质的能力被破坏的宿主细菌中表达该蛋白质(Gottesman，S.GeneExpressionTechnologyMethodsinEnzymology185，AcademicPress，SanDiego，California(1990)119-128)。另一个策略是对待插入表达载体中的核酸的核酸序列进行修饰，以致于使每一氨基酸的个别密码子为选择用于表达的细菌(如谷氨酸棒杆菌)中所优选使用的那些密码子(Wada等人，(1992)NucleicAcidRes.202111-2118)。通过DNA合成的标准技术对本发明核酸序列进行该修饰。在另一个实施方案中，MP蛋白质表达载体为酵母表达载体。用于在酿酒酵母(S.cerevisiae)中表达的载体实例包括pYepSec1(Baldari等人，(1987)EmboJ.6229-234)、pMFa(Kurjan和Herskowitz，(1982)Cell30933-943)、pJRY88(Schultz等人，(1987)Gene54113-123)和pYES2(InvitrogenCorporation，SanDiego，CA)。适用在其它真菌如丝状真菌中使用的载体及构建载体的方法包括详细描述于vandenHondel，C.A.M.J.J.和Punt，P.J.(1991)，“Genetransfersystemsandvectordevelopmentforfilamentousfungi”，在AppliedMolecularGeneticsoffungi，编者J.F.Peberdy等人，第1-28页，CambridgeUniversityPressCambridge中的那些。作为另一个替代方式，可以利用杆状病毒表达载体在昆虫细胞中表达本发明MP蛋白质。可获得的在培养昆虫细胞(如Sf9细胞)中表达蛋白质的杆状病毒载体包括pAc系列(Smith等人，(1983)Mol.CellBiol.32156-2165)和pVL系列(Lucklow和Summers(1989)Virology17031-39)。在其它实施方案中，可以在单细胞植物细胞(例如藻类)或高等植物细胞(例如诸如作物的种子植物)中表达本发明MP蛋白质。植物表达载体的实例包括详细描述于Becker，D.，Kemper，E.，Schell，J.和Masterson，R.(1992)，“Newplantbinaryvectorswithselectablemarkerslocatedproximaltotheleftborder”，PlantMol.Biol.201195-1197；以及Bevan，M.W.(1984)，“BinaryAgrobacteriumvectorsforplanttransformation”，Nucl.AcidsRes.128711-8721中的那些。在另一个实施方案中，利用哺乳动物表达载体在哺乳动物细胞中表达本发明的核酸。哺乳动物表达载体的实例包括pCDM8(Seed，B.，(1987)Nature329840)和pMT2PC(Kaufman等人，(1987)EMBOJ.6187-195)。当在哺乳动物细胞中使用时，表达载体的控制功能通常由病毒调节元件提供。常用的启动子来自如多瘤病毒、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它适宜表达系统可见于Sambrook，J.，Fritsch，E.F.和Maniatis，T.，MolecularcloningALaboratoryManual，第2版，ColdSpringHarborLaboratory，ColdSpringHarborLaboratoryPress，ColdSpringHarbor，NY，1989的第16及17章。在其它实施方案中，重组哺乳动物表达载体可引起核酸的表达，优选在特定细胞类型中表达(例如使用组织特异性调节元件来表达核酸)。组织特异性性调节元件为本领域所知。适宜组织特异性启动子的非限定性实例包括白蛋白启动子(肝脏特异性；Pinkert等人，(1987)GeneDev.1268-277)、淋巴特异性启动子(Calame和Eaton(1988)Adv.Immunol.43235-275)、特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8729-733)和免疫球蛋白启动子(Banerji等人，(1983)Cell33729-740；Queen和Baltimore(1983)Cell33741-748)、神经元特异性启动子(如神经丝启动子；Byrne和Ruddle，(1989)PNAS865473-5477)、胰腺特异性启动子(Edllund等人，(1985)Science230912-916)和乳房特异性启动子(如乳清启动子；美国专利号4873316和欧洲专利申请文献号264166)。同样也包含发育调节的启动子，例如鼠hox启动子(Kessel和Gruss，(1990)Science249374-379)和甲胎蛋白启动子(Campes和Tilghman，(1989)GenesDev.3537-546)。此外，本发明提供包含发明性DNA分子的重组表达载体，其中所述DNA分子已经以反义方向克隆入表达载体。此意味着DNA分子与调节序列功能性连接从而表达出为MPmRNA反义的RNA分子(通过DNA分子的转录)。可以选择与以反义方向克隆的核酸功能性连接并且控制反义RNA分子在多种细胞类型中连续表达的调节序列；例如可以选择控制反义RNA组成型组织特异性或细胞类型特异性表达的病毒启动子和/或增强子或调节序列。反义表达载体可为重组质粒、噬粒或减毒病毒的形式，其在高效调节区控制下产生反义核酸，所述高效调节区的活性是由载体所导入的细胞类型所决定的。通过反义基因方式对基因表达进行的调节讨论于Weintraub，H.等人，AntisenseRANasamoleculartoolforgeneticanalysis，Reviews-TrendsinGenetics，第1(1)卷，1986。本发明的另一个方面涉及本发明重组表达载体已经导入其中的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在本文中可互换使用。当然，这些术语不仅涉及特定靶细胞，而且还涉及此细胞的后代或可能的后代。由于因为突变或环境因子的原因特定修饰可能出现于连续的世代中，所以该后代没有必要与母细胞完全相同但是它仍包含于本文所使用术语的范围内。宿主细胞可为原核或真核细胞。例如，MP蛋白质可在细菌细胞如谷氨酸棒杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢细胞(CHO)或COS细胞)中表达。其它适宜宿主细胞为熟练技术人员所熟悉。与谷氨酸棒杆菌有关且以适宜方式用作本发明核酸和蛋白质分子宿主细胞的微生物列于表3。可使用常规转化或转染方法将载体DNA导入原核或真核细胞中。如本文中所使用，术语“转化”和“转染”旨在包含本领域已知的用于将外来核酸(如DNA)导入宿主细胞的多种方法，包括磷酸钙或氯化钙共沉淀、DEAE葡聚糖介导转染、脂质体转染或电穿孔。转化或转染宿主细胞的适宜方法可见于Sambrook等人的实验手册(MolecularCloningALaboratoryManual，第2版，ColdSpringHarborLaboratory，ColdSpringHarborLaboratoryPress，ColdSpringHarbor，NY，1989)以及其它实验室手册。以稳定转染哺乳动物细胞为例，已知取决于所使用的表达载体和转染技术，仅有少部分细胞将外来DNA整合入其基因组内。通常通过将编码选择标记(例如对抗生素具有抗性)的基因与目的基因一起导入宿主细胞来鉴定和选择这些整合体。优选的选择标记包括赋予对药物如G418、潮霉素和甲氨蝶呤抗性的那些。可将编码选择标记的核酸以与编码MP蛋白质的同一载体导入宿主细胞或者以不同载体导入。已用所导入核酸稳定转染的细胞可通过药物选择鉴定(例如，已整合选择标记的细胞能够存活，而其它细胞则死亡)。通过制备包含至少一个已经引入缺失、添加或替代(为了修饰或功能性破坏MP基因)MP基因片段的载体，可产生同源重组的微生物。该MP基因优选为谷氨酸棒杆菌MP基因，但也可以使用来自相关细菌或者甚至哺乳动物、酵母或昆虫来源的同系物。在优选实施方案中，将载体进行设计以致于同源重组能够功能性破坏内源MP基因(即基因不再编码功能蛋白质；也称为“敲除”载体)。作为备选方式，将载体进行设计以致于同源重组使得仍编码功能蛋白质的内源MP基因发生突变否则发生修饰(例如，可将位于上游的调节区进行修饰使得内源MP蛋白质的表达受到修饰)。MP基因的额外核酸位于同源重组载体中修饰的MP基因部分的5’端和3’端两侧，这使得载体所携带外源MP基因与微生物中的内源MP基因之间的同源重组成为可能。额外的侧翼MP核酸的长度足以使与内源基因成功进行同源重组。通常，载体包含几千个碱基的侧翼DNA(在5’和3’两端)(见例如Thomas，K.R.和Capecchi，M.R.，(1987)Cell51503中的同源重组载体描述)。将载体导如微生物(如通过电穿孔)并且使用本领域已知的方法选择其中所导入MP基因已与内源MP基因同源地重组的细胞。在另一个实施方案中，可以产生含有能够调节所导入基因表达的所选择系统的重组微生物。例如，载体中包含处于lac操作子控制下的MP基因使得MP基因仅在IPTG存在条件下表达。这些调节系统是本领域已知的。本发明的宿主细胞(如培养的真核或原核宿主细胞)可用于生产(即表达)MP蛋白质。此外，本发明提供通过利用本发明的宿主细胞生产MP蛋白质的方法。在一个实施方案中，方法包括在适宜培养基中培养本发明的宿主细胞(已将编码MP蛋白质的重组表达载体导入其中或者已将编码野生或修饰MP蛋白质的基因导入其基因组中)，直到已经生产出MP蛋白质。在其它实施方案中，方法包括从培养基或宿主细胞分离MP蛋白质。C.本发明的用途和方法本文中所述的核酸分子、蛋白质、融合蛋白质、引物、载体和宿主细胞可用于下列一种或多种方法中谷氨酸棒杆菌和相关生物的鉴定、与谷氨酸棒杆菌相关生物的基因组作图、谷氨酸棒杆菌目的序列的鉴定和定位、演化研究、MP蛋白质功能所需区域的确定、MP蛋白质活性的调节；MP途径活性的调节；以及目的化合物如精细化学品的细胞生产调节。本发明MP核酸分子具有多种用途。首先，可将其用于鉴定生物如谷氨酸棒杆菌或其密切相关物。也可将其用于从混合微生物群体中鉴定谷氨酸棒杆菌或其相关物。本发明提供许多谷氨酸棒杆菌基因的核酸序列。使用包含谷氨酸棒杆菌基因区域(在该生物中所述区域为独特的)的探针，在严格条件下对从相同或混合微生物种群培养物提取的基因组DNA进行探测，则可以确定是否存在所述生物。虽然谷氨酸棒杆菌本身为非致病菌，但其与致病菌如白喉棒杆菌有关。对此生物的检测具有重大的临床意义。本发明的核酸和蛋白质分子可作为基因组特异区域的标记物。这不仅在基因组作图中有用，而且还在用于对谷氨酸棒杆菌蛋白质的功能研究。通过例如断裂谷氨酸棒杆菌基因组并将片段与DNA-结合蛋白质孵育，来鉴定特定谷氨酸棒杆菌DNA结合蛋白质所结合的基因组区域。此外可用本发明的核酸分子对结合蛋白质的那些片段进行探测，优选通过使用容易检测的标记进行探测；此核酸分子与基因组片段的结合使得可以将片段在谷氨酸棒杆菌基因组图上进行定位，并且使用不同的酶开展此过程数次则易于蛋白质所结合核酸序列的快速确定。此外，本发明核酸分子可以与相关物种的序列充分同源以致于这些核酸分子可以作为标记物用于构建相关细菌如乳糖发酵短杆菌的基因组图。本发明的MP核酸分子同样适于演化研究和蛋白质结构研究。许多原核和真核细胞利用本发明分子所参与的代谢过程；通过比较本发明的核酸分子序列和编码来自其它生物相似酶的那些序列，可以确定该生物演化关系程度。因此，此种比较能够确定哪些序列区域是保守的并且哪些序列区域不是保守，并且这有助于确定蛋白质中对于酶功能所必需的那些区域。该类型的确定对于蛋白质工程研究是有价值的并且可以给出关于哪个蛋白质可以忍受诱变而不丧失其功能的指示。对本发明MP核酸分子的操作可造成功能不同于野生型MP蛋白质的MP蛋白质的产生。这些蛋白质在其效率或活性方面是改善了的，在细胞中以比正常量更大的量存在或者在其效率或活性方面是减弱了的。活性上的这些改变可使得来自谷氨酸棒杆菌的一种或多种精细化学品的生产量、生产和/或生产效率得到改善。通过优化激活基因(其编码目的精细化学品生物合成的蛋白质)转录或翻译的MP蛋白质活性或者通过影响或删除抑制此基因转录或翻译的MP蛋白质，由于存在增加量的例如限制酶可以使该生物合成途径的活性或活性速率增加。因此，通过修饰MP蛋白质活性使其组成型地在翻译后使涉及目的精细化学品降解途径的蛋白质失活或者通过修饰MP蛋白质活性使其组成型地抑制此基因的转录或翻译，由于降低了化合物的降解可以提高该精细化学品在细胞中的生产量和/或生产速率。由于多种代谢途径的连接，调节一种或多种MP蛋白质的活性使其能够间接刺激一种或多种精细化学品在细胞中的生产或者改善生产效率。例如，通过活化赖氨酸生物合成中的一种或多种酶的表达使生产量、生产和/或生产效率增加能够同时增加其它化合物如其它氨基酸(当需要较大量赖氨酸时细胞通常需要较大量的这些氨基酸)的表达。也可以对整个细胞的代谢调节进行修饰使得细胞在发酵培养的环境条件下(其中营养物和氧气的供应可能较差并且可能的毒性废物可能大量存在于环境中)具有改善的生长或复制。因此通过将作为对环境高水平废物的反应而抑制细胞膜产生所需分子合成的MP蛋白质(为了在亚优化生长条件下阻断细胞生长合细胞分裂)进行诱变，以致于此种蛋白质不再能够抑制该合成，从而可以改善培养细胞、甚至在亚优化生长条件下培养的细胞的生长和繁殖。此种提高的生长或此种提高的生存力同样能够提高发酵培养的目的精细化学品的生产量和/或生产速率，这是因为在培养物中相对大量的细胞产生该化合物。上述对MP蛋白质进行诱变的策略(其应该提高谷氨酸棒杆菌中目的精细化学品的生产量)并非限定性的；这些策略的变化对于熟练技术人员而言是相当明显的。在本文中所公开的这些策略和机制使得能够利用本发明的核酸和蛋白质分子以便产生表达突变的MP核酸和蛋白质分子的谷氨酸棒杆菌或相关细菌菌株，从而改善目的化合物的生产量、生产和/或生产效率。目的化合物可以是天然的谷氨酸棒杆菌产物(其包含生物合成途径的终产物和天然存在代谢途径的中间体)，还可以是谷氨酸棒杆菌代谢中天然不存在的但由本发明的谷氨酸棒杆菌菌株产生的分子。下列实施例(不应将其视为限制性的)进一步说明本发明。在本专利申请中引用的所有参考、专利申请、专利和公开专利申请的内容在此引用作为参考。实施例实施例1来自谷氨酸棒杆菌ATCC13032的全部基因组DNA的制备将谷氨酸棒杆菌(ATCC13032)在BHI培养基(Difco)中于30℃剧烈摇动培养过夜。通过离心收集细胞，将上层液丢弃并将细胞重新悬浮于5ml缓冲液I(原始培养体积的5％-所述的所有体积皆已计算成适合于100ml的培养体积)。缓冲液I的组成140.34g/l蔗糖，2.46g/lMgSO4·7H2O，10ml/lKH2PO4溶液(100g/l，用KOH调整至pH6.7)，50ml/lM12浓缩液(10g/l(NH4)2SO4，1g/lNaCl，2g/lMgSO4·7H2O，0.2g/lCaCl2，0.5g/l酵母提取物(Difco)，10ml/l痕量元素混合物(200mg/lFeSO4·H2O，10mg/lZnSO4·7H2O，3mg/lMnCl2·4H2O，30mg/lH3BO3，20mg/lCoCl2·6H2O，1mg/lNiCl2·6H2O，3mg/lNa2MoO4·2H2O)，500mg/l络合剂(EDTA或柠檬酸)，100ml/l维生素混合物(0.2ml/l生物素，0.2mg/l叶酸，20mg/l对氨基苯甲酸，20mg/l核黄素，40mg/l泛酸钙，140mg/l烟酸，40mg/l盐酸吡哆醛，200mg/l肌醇)。向悬浮液中加入终浓度2.5mg/ml的溶菌酶。于37℃孵育大约4小时后，细胞壁被降解并通过离心收集所得到的原生质体。将沉淀用5ml缓冲液I洗涤一次，再用5mlTE缓冲液(10mMTris-HCl，1mMEDTA，pH8)洗涤一次。将沉淀重新悬浮于4mlTE缓冲液中，并加入0.5mlSDS溶液(10％)和0.5mlNaCl溶液(5M)。在加入终浓度200μg/ml的蛋白酶K后，将悬浮液于37℃孵育大约18小时。通过标准方法使用苯酚、苯酚/氯仿/异戊醇和氯仿/异戊醇萃取而纯化DNA。而后加入1/50体积的3M醋酸钠和2倍体积的乙醇，随后于-20℃放置30分钟并且使用SS34转子(Sorvall)于高速离心机中以12000转/分离心30分钟将DNA沉淀。将DNA溶解于含有20μg/mlRNaseA的1mlTE缓冲液中并在4℃下对1000mlTE缓冲液透析至少3小时。在此期间将缓冲液更换3次。将0.4ml2MLiCl和0.8ml乙醇加至0.4ml透析后DNA溶液。于-20℃孵育30分钟后，通过离心(13000转/分，BiofugeFresco，Heraecus，Hanau，德国)收集DNA。将DNA沉淀溶解于TE缓冲液。通过该方法制备的DNA可用于所有目的，包括Southern印迹和构建基因组文库。实施例2在大肠杆菌中构建谷氨酸棒杆菌(ATCCl3032)基因组库根据已知且良好建立的方法(见例如Sambrook，J.等人，(1989)，“MolecularcloningALaboratoryManual”，ColdSpringHarborLaboratoryPress或Ausubel，F.M.等人，(1994)，“CurrentProtocolsinMolecularBiology”，JohnWiley&Sons)从如实施例1所述制备的DNA开始制备粘粒和质粒库。可以使用任何质粒或粘粒。特别优选的是利用质粒pBR332(Sutcliffe，J.G.，(1979)Proc.Natl.Acad.Sci.USA，753737-3741)；pACYC177(Change和Cohen，(1987)J.Bacteriol.1341141-1156)；pBS系列质粒(pBSSK+，pBSSK-及其它；Stratagene，LaJolla，美国)或粘粒如SuperCos1(Stratagene，LaJolla，美国)或Lorist6(Gibson，T.J.Rosenthal，A.和Waterson，R.H.，(1987)Gene53283-286)。实施例3DNA测序和功能计算机分析根据标准方法，特别是使用AB1377测序仪的链终止法(见例如Fleischman，R.D.等人，(1995)，“Whole-genomeRandomSequencingandAssemblyofHaemophilusInfluenzaeRd.”，Science269496-512)对如实施例2中所述的基因组库进行DNA测序。使用具有下列核苷酸序列的测序引物5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。实施例4体内诱变将质粒(或其它载体)DNA经过不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如枯草杆菌某些种(Bacillusspp.)或者酵母如酿酒酵母)，可以实现谷氨酸棒杆菌的体内诱变。一般的增变株在用于DNA修复系统的基因中含有突变(如mutHLS，mutD，mutT等，为了比较见Rupp，W.D.，(1996)DNArepairmechanismsinEscherichiacoliandSalmonella，第2277-2294页，ASMWashington)。这些株为熟练技术人员所知。这些株的用途说明于例如Greener，A.和Callahan，M.，(1994)Strategies7；32-34。实施例5DNA在大肠杆菌和谷氨酸棒杆菌间的转移多种棒杆菌和短杆菌菌株含有能自主复制的内源质粒(如例如pHM1519或pBL1)(对于综述见例如Martin，J.F.等人，(1987)Biotechnology5137-146)。通过用于大肠杆菌的标准载体(Sambrook，J.等人，(1989)，“MolecularcloningALaboratoryManual”，ColdSpringHarborLaboratoryPress或Ausubel，F.M.等人，(1994)，“CurrentProtocolsinMolecularBiology”，JohnWiley&Sons)可容易地构建用于大肠杆菌和谷氨酸棒杆菌的穿梭载体，其中向所述载体中加入了来自谷氨酸棒杆菌的复制起点和适宜标记。此复制起点优选使用已经从棒杆菌和短杆菌物种分离的内源质粒。对这些物种特别有用的转化标记物为抗卡那霉素基因(如来自Tn5或Tn903转座子的那些)或抗氯霉素基因(Winnacker，E.L.，(1987)，“FromGenestoClones-IntroductiontoGeneTechnology”，VCH，Winheim)。文献中有许多制备多种多样的能够在大肠杆菌和谷氨酸棒杆菌中复制并且可用于许多目的(包括基因过表达)的穿梭载体的实例(见例如Yoshihama，M.等人，(1985)J.Bacteriol.162591-597，Martin，J.F.等人，(1987)Biotechnology，5137-146以及Eikmanns，B.J.等人，(1992)Gene10293-98)。使用标准方法可以将目的基因克隆入上述穿梭载体之一并且可以将此种杂合载体导入谷氨酸棒杆菌菌株中。通过原生质体转化(Kastsumata，R.等人，(1984)J.Bacteriol.159，306-311)、电穿孔(Liebl，E.等人，(1989)FEMSMicrobiol.Letters，53399-303)以及使用特异载体情况下的接合(如Sch_fer，A.等人，(1990)J.Bacteriol.1721663-1666中所描述)，可转化谷氨酸棒杆菌。同样地，通过从谷氨酸棒杆菌制备质粒DNA(通过本领域已知的标准方法)并将其转化入大肠杆菌可以将穿梭载体从谷氨酸棒杆菌转移至大肠杆菌。使用标准方法可实现该转化步骤，但有利地使用Mcr缺陷大肠杆菌菌株如NM552(Gough和Murray，(1983)J.Mol.Biol.1661-19)。实施例6突变蛋白质表达的确定在转化的宿主细胞中观察突变蛋白质的活性是基于这样一种实事，即突变的蛋白质以与野生型蛋白质类似的方式和类似的量表达。确定所突变基因转录数量(指示可用于翻译成基因产物的可利用mRNA的数量)的适宜方法为实施Northern印迹(见例如Ausubel等人，(1998)，“CurrentProtocolsinMolecularBiology”，WileyNewYork)，其中所设计的能够结合目的基因的引物具有可检测(通常为放射性或化学发光)标记，以致于当从生物培养物中提取出总RNA并在凝胶上分离、转移至稳定基质并与此探针孵育时，探针的结合以及结合量指示该基因mRNA的存在以及数量。该信息指示着所突变基因已被转录的程度。可通过本领域已知的多种方法(如描述于Bormann，E.R.等人，(1992)Mol.Microbiol.6317-326)从谷氨酸棒杆菌分离总细胞RNA。通过使用标准技术如Western印迹(见例如Ausubel等人，(1998)，“CurrentProtocolsinMolecularBiology”，WileyNewYork)，可以确定从该mRNA翻译的蛋白质的存在或相对数量。在该方法中，提取总细胞蛋白质、通过凝胶电泳分离、转移至基质如硝化纤维素并且与探针孵育，所述探针例如特异性结合目的蛋白质的抗体。该探针通常具有可容易检测的化学发光标记或显色标记。标记的存在和观察到的数量指示着细胞中目的突变蛋白质的存在以及数量。实施例7经基因修饰的谷氨酸棒杆菌的生长-培养基和培养条件将经基因修饰的棒杆菌培养于合成或天然生长培养基中。用于棒杆菌的许多种不同生长培养基是已知的并且可轻易取得(Lieb等人，(1989)Appl.Microbiol.Biotechnol.32205-210；vonderOsten等人，(1998)BiotechnologyLetters1111-16；德国专利4120867；Liebl，(1992)，“TheGenusCorynebacterium”，在TheProcaryotes第II卷，编者Balows，A.Springer-Verlag)。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源为糖类如单糖、双糖或多糖。非常好的碳源实例为葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可将糖类经由复杂化合物(如糖蜜或来自糖精制的其它副产品)加至培养基。也可有利地加入不同碳源的混合物。其它可能的碳源为醇类和有机酸，如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物或含有这些化合物的材料。氮源的实例包括氨气和铵盐如NH4Cl或(NH4)2SO4、NH4OH、硝酸盐、尿素、氨基酸和复杂氮源如玉米浆、黄豆粉、黄豆蛋白质、酵母提取物、肉膏及其它。可存在于培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、磷酸盐或硫酸盐。可将螯合剂加入培养基以便保持溶液中的金属离子。特别适宜的螯合剂包括二羟基酚类如儿茶酚或原儿茶酸以及有机酸如柠檬酸。培养基通常还含有其它生长因子如维生素或生长促进剂，其实例包括生物素、核黄素、硫胺、叶酸、烟酸、泛酸及吡哆醇。生长因子及盐类常来自复杂的培养基成分如酵母提取物、糖蜜、玉米浆等。培养基的确切组成强烈地依赖于特定实验并且视个别情况而定。培养基优化信息可见于教科书“AppliedMicrobiol.Physiology，APracticalApproach”(编者P.M.Rhodes，P.F.Stanbury，IRLPress，(1997)第53-73页，ISBN0199635773)。生长培养基也可从商业供应商获得，例如Standard1(Merck)或BHI(脑心浸出液，DIFCO)等。将所有培养基成分通过加热(1.5巴和121℃下20分钟)或无菌过滤灭菌。可将成分一起灭菌或视需要分开灭菌。所有培养基成分可在培养开始即存在或视需要连续或分批加入。培养条件对每一实验分别限定。温度应为15℃和45℃之间并且自实验期间保持恒定和发生改变。培养基的pH应在5至8.5范围内，优选约7.0，并且可通过将缓冲液加入培养基而维持。用于此目的的缓冲液实例为磷酸钾缓冲液。可选择性使用或同时使用合成缓冲液如MOPS、HEPES、ACES等。在培养过程中还可以加入NaOH或NH4OH以保持pH恒定。若使用复杂培养基成分如酵母提取物，对额外缓冲液的需求量则减少，因为许多复杂化合物具有高缓冲能力。以使用发酵罐培养微生物为例，还可以利用气态氨调节pH。培养阶段通常为数小时至数天。选择该时间使得在培养液中积累最大量的产物。所公开的生长实验可在多种容器中实施，所述容器如微量滴定板、玻璃管、玻璃瓶或不同大小的玻璃或金属发酵罐。为了筛选大量克隆，应该将微生物生长于微量滴定板、玻璃管或具有或不具有档板的摇瓶中。优选使用装有10％(基于体积)的所需生长培养基的100毫升摇瓶。将摇瓶于定轨摇床(振幅25mm)上以速度100-300转/分摇动。因蒸发而造成的损失可通过保持潮湿的大气而减少；备选地，因蒸发而造成的损失可通过数学方法校正。若研究经基因修饰的克隆，则也应分析未修饰对照克隆或者含有无插入序列的基本质粒的对照克隆。使用细胞接种培养基，使OD600为0.5-1.5，其中所述细胞已经在琼脂平板如CM平板(10g/l葡萄糖，2.5g/lNaCl，2g/l尿素，10g/l聚胨，5g/l酵母提取物，5g/l肉膏，22g/l琼脂，用2MNaOH调节pH6.8)中于30℃培养。通过导入来自CM平板谷氨酸棒杆菌细胞的盐溶液或者通过加入该细菌的液体预培养物而接种于培养基中。实施例8所突变蛋白质功能的体外分析酶活性及动力学的确定是本领域众所周知的。用来确定特定经修饰酶活性的实验必须适合野生型酶的比活性，并且这处于熟练技术人员的能力范围内。关于酶的一般综述和关于结构、动力学、原理、方法、应用及确定许多酶活性的实例的特定细节可见于例如下列参考文献Dixon，M.和Webb，E.C.，(1979)Enzymes，Longmans，London；Fersht，(1985)EnzymeStructureandMechanism，Freeman，NewYork；Walsh，(1979)EnzymaticReactionMechanisms.Freeman，SanFrancisco；Price，N.C.，Stevens，L.，(1982)FundamentalsofEnzymology.OxfordUniv.PressOxford；Boyer，P.D编，(1983)，TheEnzymes，第3版，AcademicPress，NewYork；Bisswanger，H.，(1994)Enzymkinetik，第2版，VCH，Weinheim(ISBN3527300325)；Bergmeyer，H.U.，Bergmeyer，J.，Grabl，M.编，(1983-1986)，MethodsofEnzymaticAnalysis，第3版，第I-XII卷，VerlagChemieWeinheim；以及Ullmann’sEncyclopediaofIndustrialChemistry，(1987)，第A9卷，“Enzymes”，VCH，Weinheim，第352-363页。蛋白质结合DNA的活性可通过许多良好建立的方法例如DNA条带移位分析(也称为凝胶阻滞分析)测定。这些蛋白质对其它分子表达的作用可使用报道基因分析法测定(如描述于Kolmar，H.等人，(1995)EMBOJ.143895-3904及文中参考文献)。报道基因分析系统是众所周知的并且在原核和真核细胞进行了应用，其中使用了酶如β-半乳糖苷酶、绿色荧光蛋白及数种其它酶。根据描述于Gennis，R.B.，(1989)“Pores，ChannelsandTransporters”，在Biomembranes，MolecularStructureandFunction，SpringerHeidelberg，第85-137页；第199-234页和第270-322页中的技术确定膜转运蛋白质的活性。实施例9经突变蛋白质对目的产物生产的影响分析通过将所修饰微生物在适当条件下(如上述那些条件)生长并测试培养基和/或细胞成分以确定目的产物的生产的增加，可以确定谷氨酸棒杆菌中基因修饰对目的化合物(如氨基酸)生产的影响。此分析技术对熟练技术人员而言为众所周知的，并且包括光谱学、薄层层析法、多种类型的染色方法、酶和微生物方法以及分析型层析法如高效液相层析法(见例如Ullmann’sEncyclopediaofIndustrialChemistry，第A2卷，第89-90页和第443-613页，VCHWeinheim(1985)；Fallon，A.等人，(1987)，“ApplicationsofHPLCinBiochemistry”，在LaboratoryTechniquesinBiochemistryandMolecularBiology，第17卷；Rehm等人，(1993)Biotechnology，第3卷，第III章“Productrecoveryandpurification”，第469-714页，VCHWeinheim；Belter，P.A.等人，(1988)BioseparationsdownstreamprocessingforBiotechnology，JohnWileyandSons；Kennedy，J.F.和Cabral，J.M.S.，(1992)RecoveryprocessesforbiologicalMaterials，JohnWileyandSons；Shaeiwitz，J.A.和Henry，J.D.，(1988)BiochemicalSeparations，在Ullmann’sEncyclopediaofIndustrialChemistry，第B3卷，第11章，第1-27页，VCHWeinheim；以及Dechow，F.J.，(1989)Separationandpurificationtechniquesinbiotechnology，NoyesPublications)。除了测定发酵终产物以外，同样还可以分析代谢途径中用来生产目的化合物的其它成分，例如中间体和副产品，以便确定生物的总体生产力、化合物的生产量和/或生产效率。分析方法包括测定培养基中营养物的量(例如糖、碳氢化合物、氮源、磷酸盐及其它离子)、测定生物量组成及生长、分析生物合成途径的共同代谢物生产以及测定发酵期间产生的气体。用来进行这些测量的标准方法描述于AppliedMicrobialPhysiology；APracticalApproach，编者P.M.Rhodes和P.F.Stanbury，IRLPress，第103-129页、第131-163页和第165-192页(ISBN0199635773)及文中的参考文献。实施例10来自谷氨酸棒杆菌培养物的目的产物的纯化通过本领域已知的多种方法可以从谷氨酸棒杆菌细胞或上述培养物上清液中得到目的产物。若目的产物不被细胞分泌，则通过缓慢离心从培养物收集细胞，并且通过标准技术如机械力或超声裂解细胞。通过离心去除细胞碎片并得到含有可溶性蛋白质的上清液部分用于对目的化合物进一步纯化。若产物被谷氨酸棒杆菌细胞分泌，则通过缓慢离心将细胞去除并保留上清液部分用于进一步纯化。使用适当树脂将来自两种纯化方法的上清液部分进行层析，或者是目的分子滞留在层析树脂上而样品中的许多污染物不滞留在树脂上，或者是污染物滞留在树脂上而样品不滞留在树脂上。如果需要，可使用相同或不同层析树脂重复这些层析步骤数次。熟练技术人员熟悉对适当层析树脂的选择以及对于待纯化特定分子的最有效使用。通过过滤或超滤可将纯化的产物浓缩并储存于产物稳定性最高的温度下。在本技术中，许多纯化方法是已知的，其不限于上述的纯化方法并且描述于例如Bailey，J.E.和Ollis，D.F.BiochemicalEngineeringFundamentals，McGraw-HillNewYork(1986)。通过本
技术领域：
的标准技术可以确定所分离的化合物及纯度。这些技术包括高效液体层析(HPLC)、光谱方法、显色法、薄层层析、NIRS、酶分析法或微生物分析法。这些分析法汇编于Patek等人，(1994)Appl.Environ.Microbiol.60133-140；Malakhova等人，(1996)Biotekhnologiya1127-32；以及Schmidt等人，(1998)BioprocessEngineer.1967-70；Ullmann’sEncyclopediaofIndustrialChemistry(1996)，第A27卷，VCHWeinheim，第89-90页，第521-540页，第540-547页，第559-566页，第575-581页和第581-587页；Michal，G.，(1999)BiochemicalPathwaysAnAtlasofBiochemistryandMolecularBiology，JohnWileyandSons；Fallon，A.等人，(1987)ApplicationofHPLCinBiochemistry在LaboratoryTechniquesinBiochemistryandMolecularBiology，第17卷。等效物通过使用简单的常规方法，熟练技术人员知道或可以鉴定本发明特定实施方案的大量等效物。这些等效物旨在包含于下述专利权利要求范围内。表1和表2中的信息应如下理解在第1列“DNAID”中，每一情况下的有关编号是指所附序列表的SEQIDNO编号。因此，“DNAID”列中的“5”是指SEQIDNO5。在第2列“AAID”中，每一情况下的有关编号是指所附序列表的SEQIDNO编号。因此，“AAID”列中的“6”是指SEQIDNO6。在第3列“标识”中，列出每一序列的明确内部名称。在第4列“AA位置”中，每一情况下的有关编号是指同一行中“AAID”多肽序列的氨基酸位置。因此，在“AA位置”列中的“26”为对应指出的多肽序列的氨基酸位置26。位置计算自N末端以+1开始。在第5列“AA野生型”中，每一情况下的有关字母是指在第4列所指出的相应野生型的位置处的氨基酸，其以单字母代码表示。在第6列“AA突变体”中，每一情况下的有关字母是指在第4列所指出的相应突变体的位置处的氨基酸，其以单字母代码表示。在第7列“功能”中，列出了相应多肽序列的生理功能。第4、5和6列描述了具有特定功能(第7列)和特定起始氨基酸序列(第2列)的MP蛋白质的至少一种突变，在一些序列中还可以具有数个突变。所述数个突变通常指最接近的起始氨基酸序列，在每一情况下列于顶部(第2列)。术语特定氨基酸序列的“至少一个氨基酸位置”优选是指在第4、5和6列所描述的该氨基酸序列的至少一个突变。生蛋白氨基酸的单字母代码为A丙氨酸C半胱氨酸D天冬氨酸E谷氨酸F苯丙氨酸G甘氨酸H组氨酸I异亮氨酸K赖氨酸L亮氨酸M甲硫氨酸N天冬酰胺P脯氨酸Q谷氨酰胺R精氨酸S丝氨酸T苏氨酸V缬氨酸W色氨酸Y酪氨酸表1本发明的MP蛋白质表2除外的MP蛋白质序列表<110>巴斯福股份公司(BASFAktiengesellschaft)<120>用于编码精细化学品的代谢途径的蛋白质的基因变体<130>PF55186/Mec<140>AE20030360<141><160>142<210>1<211>1848<212>DNA<213>谷氨酸棒杆菌(Corynebacteriumglutamicum)<220><221>CDS<222>(1)..(1848)<223>RXA00149K<400>1ttgactgatctcacaaagactgcggtgcccgaggaactttcagagaac48MetThrAspLeuThrLysThrAlaValProGluGluLeuSerGluAsn151015ctcgaaacttggtacaaggctgtggccggtgttttcgcgcgcacacag96LeuGluThrTrpTyrLysAlaValAlaGlyValPheAlaArgThrGln202530aaaaaagacatcggcgacattgccgtagatgtgtggaagaaactcatc144LysLysAspIleGlyAspIleAlaValAspValTrpLysLysLeuIle354045gtcactacaccggatggtgttgatatcaatccgctgtacaccagagca192ValThrThrProAspGlyValAspIleAsnProLeuTyrThrArgAla505560gatgagtcccagaggaaattcactgaggttcctggtgagtttcccttc240AspGluSerGlnArgLysPheThrGluValProGlyGluPheProPhe65707580actaggggaaccactgttgatggtgaacgcgttggttggggtgttact288ThrArgGlyThrThrValAspGlyGluArgValGlyTrpGlyValThr859095gagactttcggacatgacagcccgaagaatatcaacgctgcggtgctg336GluThrPheGlyHisAspSerProLysAsnIleAsnAlaAlaValLeu100105110aatgctctgaattctggcaccaccacattgggttttgagttctctgag384AsnAlaLeuAsnSerGlyThrThrThrLeuGlyPheGluPheSerGlu115120125gaattcacggcagctgatcttaaagttgctctcgaaggcgtgtatctc432GluPheThrAlaAlaAspLeuLysValAlaLeuGluGlyValTyrLeu130135140aacatggctccgttgctgattcatgcgggtggatccacgtcagaggtt480AsnMetAlaProLeuLeuIleHisAlaGlyGlySerThrSerGluVal145150155160gcagcggcgttgtatacgttggcggaggaagccggaacgttttttgct528AlaAlaAlaLeuTyrThrLeuAlaGluGluAlaGlyThrPhePheAla165170175gcgttgaccttgggttctcgtcctttgacggcgcaggttgatggttcg576AlaLeuThrLeuGlySerArgProLeuThrAlaGlnValAspGlySer180185190cacagtgacaccattgaagaagcagttcagttggcagtgaatgcttcc624HisSerAspThrIleGluGluAlaValGlnLeuAlaValAsnAlaSer195200205aagcgtgcgaatgtgcgcgctatcttggtggatggttccagtttttcc672LysArgAlaAsnValArgAlaIleLeuValAspGlySerSerPheSer210215220aaccagggcgcgtcggatgctcaagaaattggtctaagtatcgccgcc720AsnGlnGlyAlaSerAspAlaGlnGluIleGlyLeuSerIleAlaAla225230235240ggtgtggattatgtccgtcgcttggtcgatgcaggcctttccacggaa768GlyValAspTyrValArgArgLeuValAspAlaGlyLeuSerThrGlu245250255gctgcacttaagcaggtggcgttccgttttgcggtcaccgatgagcag816AlaAlaLeuLysGlnValAlaPheArgPheAlaValThrAspGluGln260265270ttcgcgcagatttctaagctgcgtgtggctcgacgtctgtgggccagg864PheAlaGlnIleSerLysLeuArgValAlaArgArgLeuTrpAlaArg275280285gtgtgtgaggtgcttggttttccagagctggccgtagcaccacagcat912ValCysGluValLeuGlyPheProGluLeuAlaValAlaProGlnHis290295300gcggtgactgcacgagcgatgtttagccagcgtgatccgtgggtgaat960AlaValThrAlaArgAlaMetPheSerGlnArgAspProTrpValAsn305310315320atgctgcgcagtactgttgcagctttcgctgcaggcgtcggtggagca1008MetLeuArgSerThrValAlaAlaPheAlaAlaGlyValGlyGlyAla325330335accgatgtggaggttcgtacttttgatgatgcgatcccagatggagtt1056ThrAspValGluValArgThrPheAspAspAlaIleProAspGlyVal340345350cctggagtgtcgaggaatttcgctcaccgcatcgcgcgcaatactaat1104ProGlyValSerArgAsnPheAlaHisArgIleAlaArgAsnThrAsn355360365ttgttgttgctagaagagtcacatcttggtcacgtggttgatcctgct1152LeuLeuLeuLeuGluGluSerHisLeuGlyHisValValAspProAla370375380ggtggatcatatttcgtggagagcttcaccgatgatctagcggagaag1200GlyGlySerTyrPheValGluSerPheThrAspAspLeuAlaGluLys385390395400gcgtgggctgtgttcagtggcatcgaagctgagggcggatacagtgca1248AlaTrpAlaValPheSerGlyIleGluAlaGluGlyGlyTyrSerAla405410415gcttgtgcatccggcacggtgactgccatgcttgatcagacgtgggag1296AlaCysAlaSerGlyThrValThrAlaMetLeuAspGlnThrTrpGlu420425430cagactcgcgctgatgtggcgtcgagaaagaagaagctcactggaatt1344GlnThrArgAlaAspValAlaSerArgLysLysLysLeuThrGlyIle435440445aatgagttcccgaacttggcggagtctccgctgccagctgatcgtcgg1392AsnGluPheProAsnLeuAlaGluSerProLeuProAlaAspArgArg450455460gtagaacctgcaggtgtgcgtcgatgggcagcggattttgaagcgctg1440ValGluProAlaGlyValArgArgTrpAlaAlaAspPheGluAlaLeu465470475480cgcaatcgttcggatgctttcttggaaaagaacggcgcgaggccacag1488ArgAsnArgSerAspAlaPheLeuGluLysAsnGlyAlaArgProGln485490495atcacgatgattcctctgggaccgttgtccaagcacaatattcgcact1536IleThrMetIleProLeuGlyProLeuSerLysHisAsnIleArgThr500505510ggttttacttccaacctgttggcttccggtggcattgaagcaatcaac1584GlyPheThrSerAsnLeuLeuAlaSerGlyGlyIleGluAlaIleAsn515520525ccgggtcaacttgttcccggcactgacgcttttgcagaagctgcacag1632ProGlyGlnLeuValProGlyThrAspAlaPheAlaGluAlaAlaGln530535540gccgcaggcattgtagtggtgtgtggaacggaccaagagtatgccgaa1680AlaAlaGlyIleValValValCysGlyThrAspGlnGluTyrAlaGlu545550555560acgggggagggagccgtcgaaaagctccgcgaagcgggcgttgagcgc1728ThrGlyGluGlyAlaValGluLysLeuArgGluAlaGlyValGluArg565570575atcctgcttgctggcgcgccgaagagctttgagggcagcgcgcatgcg1776IleLeuLeuAlaGlyAlaProLysSerPheGluGlySerAlaHisAla580585590cccgatggttatttgaacatgacaattgatgccgcggcgacgctggct1824ProAspGlyTyrLeuAsnMetThrIleAspAlaAlaAlaThrLeuAla595600605gacctgctagatgctttgggagct1848AspLeuLeuAspAlaLeuGlyAla610615<210>2<211>616<212>PRT<213>谷氨酸棒杆菌<400>2MetThrAspLeuThrLysThrAlaValProGluGluLeuSerGluAsn151015LeuGluThrTrpTyrLysAlaValAlaGlyValPheAlaArgThrGln202530LysLysAspIleGlyAspIleAlaValAspValTrpLysLysLeuIle354045ValThrThrProAspGlyValAspIleAsnProLeuTyrThrArgAla505560AspGluSerGlnArgLysPheThrGluValProGlyGluPheProPhe65707580ThrArgGlyThrThrValAspGlyGluArgValGlyTrpGlyValThr859095GluThrPheGlyHisAspSerProLysAsnIleAsnAlaAlaValLeu100105110AsnAlaLeuAsnSerGlyThrThrThrLeuGlyPheGluPheSerGlu115120125GluPheThrAlaAlaAspLeuLysValAlaLeuGluGlyValTyrLeu130135140AsnMetAlaProLeuLeuIleHisAlaGlyGlySerThrSerGluVal145150155160AlaAlaAlaLeuTyrThrLeuAlaGluGluAlaGlyThrPhePheAla165170175AlaLeuThrLeuGlySerArgProLeuThrAlaGlnValAspGlySer180185190HisSerAspThrIleGluGluAlaValGlnLeuAlaValAsnAlaSer195200205LysArgAlaAsnValArgAlaIleLeuValAspGlySerSerPheSer210215220AsnGlnGlyAlaSerAspAlaGlnGluIleGlyLeuSerIleAlaAla225230235240GlyValAspTyrValArgArgLeuValAspAlaGlyLeuSerThrGlu245250255AlaAlaLeuLysGlnValAlaPheArgPheAlaValThrAspGluGln260265270PheAlaGlnIleSerLysLeuArgValAlaArgArgLeuTrpAlaArg275280285ValCysGluValLeuGlyPheProGluLeuAlaValAlaProGlnHis290295300AlaValThrAlaArgAlaMetPheSerGlnArgAspProTrpValAsn305310315320MetLeuArgSerThrValAlaAlaPheAlaAlaGlyValGlyGlyAla325330335ThrAspValGluValArgThrPheAspAspAlaIleProAspGlyVal340345350ProGlyValSerArgAsnPheAlaHisArgIleAlaArgAsnThrAsn355360365LeuLeuLeuLeuGluGluSerHisLeuGlyHisValValAspProAla370375380GlyGlySerTyrPheValGluSerPheThrAspAspLeuAlaGluLys385390395400AlaTrpAlaValPheSerGlyIleGluAlaGluGlyGlyTyrSerAla405410415AlaCysAlaSerGlyThrValThrAlaMetLeuAspGlnThrTrpGlu420425430GlnThrArgAlaAspValAlaSerArgLysLysLysLeuThrGlyIle435440445AsnGluPheProAsnLeuAlaGluSerProLeuProAlaAspArgArg450455460ValGluProAlaGlyValArgArgTrpAlaAlaAspPheGluAlaLeu465470475480ArgAsnArgSerAspAlaPheLeuGluLysAsnGlyAlaArgProGln485490495IleThrMetIleProLeuGlyProLeuSerLysHisAsnIleArgThr500505510GlyPheThrSerAsnLeuLeuAlaSerGlyGlyIleGluAlaIleAsn515520525ProGlyGlnLeuValProGlyThrAspAlaPheAlaGluAlaAlaGln530535540AlaAlaGlyIleValValValCysGlyThrAspGlnGluTyrAlaGlu545550555560ThrGlyGluGlyAlaValGluLysLeuArgGluAlaGlyValGluArg565570575IleLeuLeuAlaGlyAlaProLysSerPheGluGlySerAlaHisAla580585590ProAspGlyTyrLeuAsnMetThrIleAspAlaAlaAlaThrLeuAla595600605AspLeuLeuAspAlaLeuGlyAla610615<210>3<211>1890<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1890)<223>RXA00157E<400>3gtggccaatctagattcagttcgccctggggtgcgggcagctcttcga48MetAlaAsnLeuAspSerValArgProGlyValArgAlaAlaLeuArg151015cgtacccatacccaggtaaaaggtgtcacgttgaccagggcgttgatt96ArgThrHisThrGlnValLysGlyValThrLeuThrArgAlaLeuIle202530gcgcttgcagtaagcggagctttgcttagttccatgactccggcggtg144AlaLeuAlaValSerGlyAlaLeuLeuSerSerMetThrProAlaVal354045gcgcagccacagaatccggatgacgcagccattgcacaggcagaggaa192AlaGlnProGlnAsnProAspAspAlaAlaIleAlaGlnAlaGluGlu505560aatgittcggcgggcgatggggaagtcgcccgcctggcaggatctttg240AsnValSerAlaGlyAspGlyGluValAlaArgLeuAlaGlySerLeu65707580tccagcactgacgcggaaattaaccgcgtcgagctggaaatgggtgct288SerSerThrAspAlaGluIleAsnArgValGluLeuGluMetGlyAla859095ctgcgtgaagaagtgaacaagtccctcgtggatttgcatgatgcgcag336LeuArgGluGluValAsnLysSerLeuValAspLeuHisAspAlaGln100105110gcaatcgccgagcaggcccgccaagatgcacttgcagccaagaaggat384AlaIleAlaGluGlnAlaArgGlnAspAlaLeuAlaAlaLysLysAsp115120125ctcgatgattctcaagcgcagatcgaagcagcccaagagcgccttgat432LeuAspAspSerGlnAlaGlnIleGluAlaAlaGlnGluArgLeuAsp130135140gagatttcacgtgcagcgtatcgccaaaacggaacctccaaggggctt480GluIleSerArgAlaAlaTyrArgGlnAsnGlyThrSerLysGlyLeu145150155160tcaggcatctcgggcaatggaaattctgaagatgcgctagatcgtcag528SerGlyIleSerGlyAsnGlyAsnSerGluAspAlaLeuAspArgGln165170175acttacctgcgaaccagtgcggaaaagcagcaggcagctgttgaagag576ThrTyrLeuArgThrSerAlaGluLysGlnGlnAlaAlaValGluGlu180185190cttgatcgcctccgtacggaaaacgccaacaaggaatcggtgttgcgc624LeuAspArgLeuArgThrGluAsnAlaAsnLysGluSerValLeuArg195200205caggcccgcatcgttgctgagcagcgtgaggcggaagccgtcgaaaag672GlnAlaArgIleValAlaGluGlnArgGluAlaGluAlaValGluLys210215220caagtccagaccgaggctgcaattgccgcaaacagcgagcagctcaat720GlnValGlnThrGluAlaAlaIleAlaAlaAsnSerGluGlnLeuAsn225230235240gtcttgactaacaatcgcagtaccttggttgcccagcgtgatggggct768ValLeuThrAsnAsnArgSerThrLeuValAlaGlnArgAspGlyAla245250255gagcgcaacttggccatcgctcgtgcgcaggcggataatctgcaaggt816GluArgAsnLeuAlaIleAlaArgAlaGlnAlaAspAsnLeuGlnGly260265270cagcgtgctgagtacgaggaattccagcaggcagagcaggctcgcatc864GlnArgAlaGluTyrGluGluPheGlnGlnAlaGluGlnAlaArgIle275280285caggcggaagcggaagctcaggctgctgcggaggagaagcgtcgtgcc912GlnAlaGluAlaGluAlaGlnAlaAlaAlaGluGluLysArgArgAla290295300gatgaggctgctgcacaggcagccgctgaagctcaagaagctgcccag960AspGluAlaAlaAlaGlnAlaAlaAlaGluAlaGlnGluAlaAlaGln305310315320caagctcaggcggcggaggaagcccaagccgcgcaagcagctgagaca1008GlnAlaGlnAlaAlaGluGluAlaGlnAlaAlaGlnAlaAlaGluThr325330335gcacaagcccaagccgcgcaagctgcggaaacccaagctgcacaagcc1056AlaGlnAlaGlnAlaAlaGlnAlaAlaGluThrGlnAlaAlaGlnAla340345350gcgcaagctcaggcagaagcgaatgatcgtgccgccgcgcaacagcgt1104AlaGlnAlaGlnAlaGluAlaAsnAspArgAlaAlaAlaGlnGlnArg355360365gctgcagaggctcaagcagcagcggaacaggcgcaacgtgaggctgac1152AlaAlaGluAlaGlnAlaAlaAlaGluGlnAlaGlnArgGluAlaAsp370375380gctcaggcggccaacgatgcccaagctcaggcactgcgtgaacaggcg1200AlaGlnAlaAlaAsnAspAlaGlnAlaGlnAlaLeuArgGluGlnAla385390395400ctcaccgcagcctccatcgctgcggctgctctaattgcggcgagccag1248LeuThrAlaAlaSerIleAlaAlaAlaAlaLeuIleAlaAlaSerGln405410415tccagccatgccactactcaaaatccttacccaactgatgaagacgcg1296SerSerHisAlaThrThrGlnAsnProTyrProThrAspGluAspAla420425430gatccgaccgatattgcggacatccaaggcccaacgcagccaggtacg1344AspProThrAspIleAlaAspIleGlnGlyProThrGlnProGlyThr435440445ggtgagtctggagattcccagagcaactccagcgacaacgattccaca1392GlyGluSerGlyAspSerGlnSerAsnSerSerAspAsnAspSerThr450455460ggcaacgattccacaggctctgactcttcagattcagattcctccggc1440GlyAsnAspSerThrGlySerAspSerSerAspSerAspSerSerGly465470475480aacgattcttcagaggttatttccggcgatcgttccgctcagattgag1488AsnAspSerSerGluValIleSerGlyAspArgSerAlaGlnIleGlu485490495actgtgattgcgcgcgccatgagccagttgggtgtgcagtacgcatgg1536ThrValIleAlaArgAlaMetSerGlnLeuGlyValGlnTyrAlaTrp500505510ggtggcggtaacgctaatggcccaactctgggtatccgtgacggtggc1584GlyGlyGlyAsnAlaAsnGlyProThrLeuGlyIleArgAspGlyGly515520525gtggcggactcttacggcgattacaacaaggttggcttcgactgctct1632ValAlaAspSerTyrGlyAspTyrAsnLysValGlyPheAspCysSer530535540ggactgaccttgtatgcgtttgcgggtgtgggaatttcacttcctcac1680GlyLeuThrLeuTyrAlaPheAlaGlyValGlyIleSerLeuProHis545550555560tacacgggctaccagtaccagcacggcaccaaggtgtcgccttctgag1728TyrThrGlyTyrGlnTyrGlnHisGlyThrLysValSerProSerGlu565570575atgcaacgtggcgatctgatcttctatggtccgggagcgtctcagcac1776MetGlnArgGlyAspLeuIlePheTyrGlyProGlyAlaSerGlnHis580585590gtggcaatttacctcggtgatggtcagatgattgaggctccgaattcg1824ValAlaIleTyrLeuGlyAspGlyGlnMetIleGluAlaProAsnSer595600605ggttctgtcgtgaagatttctcctgttcgctggagcggaatgaccgag1872GlySerValValLysIleSerProValArgTrpSerGlyMetThrGlu610615620agcgtggtacgcctcatt1890SerValValArgLeuIle625630<210>4<211>630<212>PRT<213>谷氨酸棒杆菌<400>4MetAlaAsnLeuAspSerValArgProGlyValArgAlaAlaLeuArg151015ArgThrHisThrGlnValLysGlyValThrLeuThrArgAlaLeuIle202530AlaLeuAlaValSerGlyAlaLeuLeuSerSerMetThrProAlaVal354045AlaGlnProGlnAsnProAspAspAlaAlaIleAlaGlnAlaGluGlu505560AsnValSerAlaGlyAspGlyGluValAlaArgLeuAlaGlySerLeu65707580SerSerThrAspAlaGluIleAsnArgValGluLeuGluMetGlyAla859095LeuArgGluGluValAsnLysSerLeuValAspLeuHisAspAlaGln100105110AlaIleAlaGluGlnAlaArgGlnAspAlaLeuAlaAlaLysLysAsp115120125LeuAspAspSerGlnAlaGlnIleGluAlaAlaGlnGluArgLeuAsp130135140GluIleSerArgAlaAlaTyrArgGlnAsnGlyThrSerLysGlyLeu145150155160SerGlyIleSerGlyAsnGlyAsnSerGluAspAlaLeuAspArgGln165170175ThrTyrLeuArgThrSerAlaGluLysGlnGlnAlaAlaValGluGlu180185190LeuAspArgLeuArgThrGluAsnAlaAsnLysGluSerValLeuArg195200205GlnAlaArgIleValAlaGluGlnArgGluAlaGluAlaValGluLys210215220GlnValGlnThrGluAlaAlaIleAlaAlaAsnSerGluGlnLeuAsn225230235240ValLeuThrAsnAsnArgSerThrLeuValAlaGlnArgAspGlyAla245250255GluArgAsnLeuAlaIleAlaArgAlaGlnAlaAspAsnLeuGlnGly260265270GlnArgAlaGluTyrGluGluPheGlnGlnAlaGluGlnAlaArgIle275280285GlnAlaGluAlaGluAlaGlnAlaAlaAlaGluGluLysArgArgAla290295300AspGluAlaAlaAlaGlnAlaAlaAlaGluAlaGlnGluAlaAlaGln305310315320GlnAlaGlnAlaAlaGluGluAlaGlnAlaAlaGlnAlaAlaGluThr325330335AlaGlnAlaGlnAlaAlaGlnAlaAlaGluThrGlnAlaAlaGlnAla340345350AlaGlnAlaGlnAlaGluAlaAsnAspArgAlaAlaAlaGlnGlnArg355360365AlaAlaGluAlaGlnAlaAlaAlaGluGlnAlaGlnArgGluAlaAsp370375380AlaGlnAlaAlaAsnAspAlaGlnAlaGlnAlaLeuArgGluGlnAla385390395400LeuThrAlaAlaSerIleAlaAlaAlaAlaLeuIleAlaAlaSerGln405410415SerSerHisAlaThrThrGlnAsnProTyrProThrAspGluAspAla420425430AspProThrAspIleAlaAspIleGlnGlyProThrGlnProGlyThr435440445GlyGluSerGlyAspSerGlnSerAsnSerSerAspAsnAspSerThr450455460GlyAsnAspSerThrGlySerAspSerSerAspSerAspSerSerGly465470475480AsnAspSerSerGluValIleSerGlyAspArgSerAlaGlnIleGlu485490495ThrValIleAlaArgAlaMetSerGlnLeuGlyValGlnTyrAlaTrp500505510GlyGlyGlyAsnAlaAsnGlyProThrLeuGlyIleArgAspGlyGly515520525ValAlaAspSerTyrGlyAspTyrAsnLysValGlyPheAspCysSer530535540GlyLeuThrLeuTyrAlaPheAlaGlyValGlyIleSerLeuProHis545550555560TyrThrGlyTyrGlnTyrGlnHisGlyThrLysValSerProSerGlu565570575MetGlnArgGlyAspLeuIlePheTyrGlyProGlyAlaSerGlnHis580585590ValAlaIleTyrLeuGlyAspGlyGlnMetIleGluAlaProAsnSer595600605GlySerValValLysIleSerProValArgTrpSerGlyMetThrGlu610615620SerValValArgLeuIle625630<210>5<211>1800<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1800)<223>RXA00157K<400>5ttgattgcgcttgcagtaagcggagctttgcttagttccatgactccg48MetIleAlaLeuAlaValSerGlyAlaLeuLeuSerSerMetThrPro151015gcggtggcgcagccacagaatccggatgacgcagccattgcacaggca96AlaValAlaGlnProGlnAsnProAspAspAlaAlaIleAlaGlnAla202530gaggaaaatgtttcggcgggcgatggggaagtcgcccgcctggcagga144GluGluAsnValSerAlaGlyAspGlyGluValAlaArgLeuAlaGly354045tctttgtccagcactgacgcggaaattaaccgcgtcgagctggaaatg192SerLeuSerSerThrAspAlaGluIleAsnArgValGluLeuGluMet505560ggtgctctgcgtgaagaagtgaacaagtccctcgtggatttgcatgat240GlyAlaLeuArgGluGluValAsnLysSerLeuValAspLeuHisAsp65707580gcgcaggcaatcgccgagcaggcccgccaagatgcacttgcagccaag288AlaGlnAlaIleAlaGluGlnAlaArgGlnAspAlaLeuAlaAlaLys859095aaggatctcgatgattctcaagcgcagatcgaagcagcccaagagcgc336LysAspLeuAspAspSerGlnAlaGlnIleGluAlaAlaGlnGluArg100105110cttgatgagatttcacgtgcagcgtatcgccaaaacggaacctccaag384LeuAspGluIleSerArgAlaAlaTyrArgGlnAsnGlyThrSerLys115120125gggctttcaggcatctcgggcaatggaaattctgaagatgcgctagat432GlyLeuSerGlyIleSerGlyAsnGlyAsnSerGluAspAlaLeuAsp130135140cgtcagacttacctgcgaaccagtgcggaaaagcagcaggcagctgtt480ArgGlnThrTyrLeuArgThrSerAlaGluLysGlnGlnAlaAlaVal145150155160gaagagcttgatcgcctccgtacggaaaacgccaacaaggaatcggtg528GluGluLeuAspArgLeuArgThrGluAsnAlaAsnLysGluSerVal165170175ttgcgccaggcccgcatcgttgctgagcagcgtgaggcggaagccgtc576LeuArgGlnAlaArgIleValAlaGluGlnArgGluAlaGluAlaVal180185190gaaaagcaagtccagaccgaggctgcaattgccgcaaacagcgagcag624GluLysGlnValGlnThrGluAlaAlaIleAlaAlaAsnSerGluGln195200205ctcaatgtcttgactaacaatcgcagtaccttggttgcccagcgtgat672LeuAsnValLeuThrAsnAsnArgSerThrLeuValAlaGlnArgAsp210215220ggggctgagcgcaacttggccatcgctcgtgcgcaggcggataatctg720GlyAlaGluArgAsnLeuAlaIleAlaArgAlaGlnAlaAspAsnLeu225230235240caaggtcagcgtgctgagtacgaggaattccagcaggcagagcaggct768GlnGlyGlnArgAlaGluTyrGluGluPheGlnGlnAlaGluGlnAla245250255cgcatccaggcggaagcggaagctcaggctgctgcggaggagaagcgt816ArgIleGlnAlaGluAlaGluAlaGlnAlaAlaAlaGluGluLysArg260265270cgtgccgatgaggctgctgcacaggcagccgctgaagctcaagaagct864ArgAlaAspGluAlaAlaAlaGlnAlaAlaAlaGluAlaGlnGluAla275280285gcccagcaagctcaggcggcggaggaagcccaagccgcgcaagcagct912AlaGlnGlnAlaGlnAlaAlaGluGluAlaGlnAlaAlaGlnAlaAla290295300gagacagcacaagcccaagccgcgcaagctgcggaaacccaagctgca960GluThrAlaGlnAlaGlnAlaAlaGlnAlaAlaGluThrGlnAlaAla305310315320caagccgcgcaagctcaggcagaagcgaatgatcgtgccgccgcgcaa1008GlnAlaAlaGlnAlaGlnAlaGluAlaAsnAspArgAlaAlaAlaGln325330335cagcgtgctgcagaggctcaagcagcagcggaacaggcgcaacgtgag1056GlnArgAlaAlaGluAlaGlnAlaAlaAlaGluGlnAlaGlnArgGlu340345350gctgacgctcaggcggccaacgatgcccaagctcaggcactgcgtgaa1104AlaAspAlaGlnAlaAlaAsnAspAlaGlnAlaGlnAlaLeuArgGlu355360365caggcgctcaccgcagcctccatcgctgcggctgctctaattgcggcg1152GlnAlaLeuThrAlaAlaSerIleAlaAlaAlaAlaLeuIleAlaAla370375380agccagtccagccatgccactactcaaaatccttacccaactgatgaa1200SerGlnSerSerHisAlaThrThrGlnAsnProTyrProThrAspGlu385390395400gacgcggatccgaccgatattgcggacatccaaggcccaacgcagcca1248AspAlaAspProThrAspIleAlaAspIleGlnGlyProThrGlnPro405410415ggtacgggtgagtctggagattcccagagcaactccagcgacaacgat1296GlyThrGlyGluSerGlyAspSerGlnSerAsnSerSerAspAsnAsp420425430tccacaggcaacgattccacaggctctgactcttcagattcagattcc1344SerThrGlyAsnAspSerThrGlySerAspSerSerAspSerAspSer435440445tccggcaacgattcttcagaggttatttccggcgatcgttccgctcag1392SerGlyAsnAspSerSerGluValIleSerGlyAspArgSerAlaGln450455460attgagactgtgattgcgcgcgccatgagccagttgggtgtgcagtac1440IleGluThrValIleAlaArgAlaMetSerGlnLeuGlyValGlnTyr465470475480gcatggggtggcggtaacgctaatggcccaactctgggtatccgtgac1488AlaTrpGlyGlyGlyAsnAlaAsnGlyProTnrLeuGlyIleArgAsp485490495ggtggcgtggcggactcttacggcgattacaacaaggttggcttcgac1536GlyGlyValAlaAspSerTyrGlyAspTyrAsnLysValGlyPheAsp500505510tgctctggactgaccttgtatgcgtttgcgggtgtgggaatttcactt1584CysSerGlyLeuThrLeuTyrAlaPheAlaGlyValGlyIleSerLeu515520525cctcactacacgggctaccagtaccagcacggcaccaaggtgtcgcct1632ProHisTyrThrGlyTyrGlnTyrGlnHisGlyThrLysValSerPro530535540tctgagatgcaacgtggcgatctgatcttctatggtccgggagcgtct1680SerGluMetGlnArgGlyAspLeuIlePheTyrGlyProGlyAlaSer545550555560cagcacgtggcaatttacctcggtgatggtcagatgattgaggctccg1728GlnHisValAlaIleTyrLeuGlyAspGlyGlnMetIleGluAlaPro565570575aattcgggttctgtcgtgaagatttctcctgttcgctggagcggaatg1776AsnSerGlySerValValLysIleSerProValArgTrpSerGlyMet580585590accgagagcgtggtacgcctcatt1800ThrGluSerValValArgLeuIle595600<210>6<211>600<212>PRT<213>谷氨酸棒杆菌<400>6MetIleAlaLeuAlaValSerGlyAlaLeuLeuSerSerMetThrPro151015AlaValAlaGlnProGlnAsnProAspAspAlaAlaIleAlaGlnAla202530GluGluAsnValSerAlaGlyAspGlyGluValAlaArgLeuAlaGly354045SerLeuSerSerThrAspAlaGluIleAsnArgValGluLeuGluMet505560GlyAlaLeuArgGluGluValAsnLysSerLeuValAspLeuHisAsp65707580AlaGlnAlaIleAlaGluGlnAlaArgGlnAspAlaLeuAlaAlaLys859095LysAspLeuAspAspSerGlnAlaGlnIleGluAlaAlaGlnGluArg100105110LeuAspGluIleSerArgAlaAlaTyrArgGlnAsnGlyThrSerLys115120125GlyLeuSerGlyIleSerGlyAsnGlyAsnSerGluAspAlaLeuAsp130135140ArgGlnThrTyrLeuArgThrSerAlaGluLysGlnGlnAlaAlaVal145150155160GluGluLeuAspArgLeuArgThrGluAsnAlaAsnLysGluSerVal165170175LeuArgGlnAlaArgIleValAlaGluGlnArgGluAlaGluAlaVal180185190GluLysGlnValGlnThrGluAlaAlaIleAlaAlaAsnSerGluGln195200205LeuAsnValLeuThrAsnAsnArgSerThrLeuValAlaGlnArgAsp210215220GlyAlaGluArgAsnLeuAlaIleAlaArgAlaGlnAlaAspAsnLeu225230235240GlnGlyGlnArgAlaGluTyrGluGluPheGlnGlnAlaGluGlnAla245250255ArgIleGlnAlaGluAlaGluAlaGlnAlaAlaAlaGluGluLysArg260265270ArgAlaAspGluAlaAlaAlaGlnAlaAlaAlaGluAlaGlnGluAla275280285AlaGlnGlnAlaGlnAlaAlaGluGluAlaGlnAlaAlaGlnAlaAla290295300GluThrAlaGlnAlaGlnAlaAlaGlnAlaAlaGluThrGlnAlaAla305310315320GlnAlaAlaGlnAlaGlnAlaGluAlaAsnAspArgAlaAlaAlaGln325330335GlnArgAlaAlaGluAlaGlnAlaAlaAlaGluGlnAlaGlnArgGlu340345350AlaAspAlaGlnAlaAlaAsnAspAlaGlnAlaGlnAlaLeuArgGlu355360365GlnAlaLeuThrAlaAlaSerIleAlaAlaAlaAlaLeuIleAlaAla370375380SerGlnSerSerHisAlaThrThrGlnAsnProTyrProThrAspGlu385390395400AspAlaAspProThrAspIleAlaAspIleGlnGlyProThrGlnPro405410415GlyThrGlyGluSerGlyAspSerGlnSerAsnSerSerAspAsnAsp420425430SerThrGlyAsnAspSerThrGlySerAspSerSerAspSerAspSer435440445SerGlyAsnAspSerSerGluValIleSerGlyAspArgSerAlaGln450455460IleGluThrValIleAlaArgAlaMetSerGlnLeuGlyValGlnTyr465470475480AlaTrpGlyGlyGlyAsnAlaAsnGlyProThrLeuGlyIleArgAsp485490495GlyGlyValAlaAspSerTyrGlyAspTyrAsnLysValGlyPheAsp500505510CysSerGlyLeuThrLeuTyrAlaPheAlaGlyValGlyIleSerLeu515520525ProHisTyrThrGlyTyrGlnTyrGlnHisGlyThrLysValSerPro530535540SerGluMetGlnArgGlyAspLeuIlePheTyrGlyProGlyAlaSer545550555560GlnHisValAlaIleTyrLeuGlyAspGlyGlnMetIleGluAlaPro565570575AsnSerGlySerValValLysIleSerProValArgTrpSerGlyMet580585590ThrGluSerValValArgLeuIle595600<210>7<211>1485<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1485)<223>RXA00330W<400>7gtgggggagtattgtgtcaccccttgggatagggttatatccgtggac48MetGlyGluTyrCysValThrProTrpAspArgValIleSerValAsp151015tacatttcgacgcgtgatgccagccgtacccctgcccgcttcagtgat96TyrIleSerThrArgAspAlaSerArgThrProAlaArgPheSerAsp202530attttgctgggcggtctagcaccagacggcggcctgtacctgcctgca144IleLeuLeuGlyGlyLeuAlaProAspGlyGlyLeuTyrLeuProAla354045acctaccctcaactagatgatgcccagctgagtaaatggcgtgaggta192ThrTyrProGlnLeuAspAspAlaGlnLeuSerLysTrpArgGluVal505560ttagccaacgaaggatacgcagctttggctgctgaagttatctccctg240LeuAlaAsnGluGlyTyrAlaAlaLeuAlaAlaGluValIleSerLeu65707580tttgttgatgacatcccagtagaagacatcaaggcgatcaccgcacgc288PheValAspAspIleProValGluAspIleLysAlaIleThrAlaArg859095gcctacacctacccgaagttcaacagcgaagacatcgttcctgtcacc336AlaTyrThrTyrProLysPheAsnSerGluAspIleValProValThr100105110gaactcgaggacaacatttacctgggccacctttccgaaggcccaacc384GluLeuGluAspAsnIleTyrLeuGlyHisLeuSerGluGlyProThr115120125gctgcattcaaagacatggccatgcagctgctcggcgaacttttcgaa432AlaAlaPheLysAspMetAlaMetGlnLeuLeuGlyGluLeuPheGlu130135140tacgagcttcgccgccgcaacgaaaccatcaacatcctgggcgctacc480TyrGluLeuArgArgArgAsnGluThrIleAsnIleLeuGlyAlaThr145150155160tctggcgataccggctcctctgcggaatacgccatgcgcggccgcgag528SerGlyAspThrGlySerSerAlaGluTyrAlaMetArgGlyArgGlu165170175ggaatccgcgtattcatgctgaccccagctggccgcatgaccccattc576GlyIleArgValPheMetLeuThrProAlaGlyArgMetThrProPhe180185190cagcaagcacagatgtttggccttgacgatccaaacatcttcaacatc624GlnGlnAlaGlnMetPheGlyLeuAspAspProAsnIlePheAsnIle195200205gccctcgacggcgttttcgacgattgccaagacgtagtcaaggctgtc672AlaLeuAspGlyValPheAspAspCysGlnAspValValLysAlaVal210215220tccgccgacgcagaattcaaaaaagacaaccgcatcggtgccgtgaac720SerAlaAspAlaGluPheLysLysAspAsnArgIleGlyAlaValAsn225230235240tccatcaactgggcacgccttatggcacaggttgtgtactacgtttcc768SerIleAsnTrpAlaArgLeuMetAlaGlnValValTyrTyrValSer245250255tcatggatccgcaccacaaccagcaatgaccaaaaggtcagcttctcc816SerTrpIleArgThrThrThrSerAsnAspGlnLysValSerPheSer260265270gtaccaaccggcaacttcggtgacatttgcgcaggccacatcgcccgc864ValProThrGlyAsnPheGlyAspIleCysAlaGlyHisIleAlaArg275280285caaatgggacttcccatcgatcgcctcatcgtggccaccaacgaaaac912GlnMetGlyLeuProIleAspArgLeuIleValAlaThrAsnGluAsn290295300gatgtgctcgacgagttcttccgtaccggcgactaccgagtccgcagc960AspValLeuAspGluPhePheArgThrGlyAspTyrArgValArgSer305310315320tccgcagacacccacgagacctcctcaccttcgatggatatctcccgc1008SerAlaAspThrHisGluThrSerSerProSerMetAspIleSerArg325330335gcctccaacttcgagcgtttcatcttcgacctgctcggccgcgacgcc1056AlaSerAsnPheGluArgPheIlePheAspLeuLeuGlyArgAspAla340345350acccgcgtcaacgatctatttggtacccaggttcgccaaggcggattc1104ThrArgValAsnAspLeuPheGlyThrGlnValArgGlnGlyGlyPhe355360365tcactggctgatgacgccaactttgagaaggctgcagcagaatacggt1152SerLeuAlaAspAspAlaAsnPheGluLysAlaAlaAlaGluTyrGly370375380ttcgcctccggacgatccacccatgctgaccgtgtggcaaccatcgct1200PheAlaSerGlyArgSerThrHisAlaAspArgValAlaThrIleAla385390395400gacgtgcattcccgcctcgacgtactaatcgatccccacaccgccgac1248AspValHisSerArgLeuAspValLeuIleAspProHisThrAlaAsp405410415ggcgttcacgtggcacgccagtggagggacgaggtcaacaccccaatc1296GlyValHisValAlaArgGlnTrpArgAspGluValAsnThrProIle420425430atcgtcctagaaactgcactcccagtgaaatttgccgacaccatcgtc1344IleValLeuGluThrAlaLeuProValLysPheAlaAspThrIleVal435440445gaagcaattggtgaagcacctcaaactccagagcgtttcgccgcgatc1392GluAlaIleGlyGluAlaProGlnThrProGluArgPheAlaAlaIle450455460atggatgctccattcaaggtttccgacctaccaaacgacaccgatgca1440MetAspAlaProPheLysValSerAspLeuProAsnAspThrAspAla465470475480gttaagcagtacatagtcgatgcgattgcaaacacttccgtgaag1485ValLysGlnTyrIleValAspAlaIleAlaAsnThrSerValLys485490495<210>8<211>495<212>PRT<213>谷氨酸棒杆菌<400>8MetGlyGluTyrCysValThrProTrpAspArgValIleSerValAsp151015TyrIleSerThrArgAspAlaSerArgThrProAlaArgPheSerAsp202530IleLeuLeuGlyGlyLeuAlaProAspGlyGlyLeuTyrLeuProAla354045ThrTyrProGlnLeuAspAspAlaGlnLeuSerLysTrpArgGluVal505560LeuAlaAsnGluGlyTyrAlaAlaLeuAlaAlaGluValIleSerLeu65707580PheValAspAspIleProValGluAspIleLysAlaIleThrAlaArg859095AlaTyrThrTyrProLysPheAsnSerGluAspIleValProValThr100105110GluLeuGluAspAsnIleTyrLeuGlyHisLeuSerGluGlyProThr115120125AlaAlaPheLysAspMetAlaMetGlnLeuLeuGlyGluLeuPheGlu130135140TyrGluLeuArgArgArgAsnGluThrIleAsnIleLeuGlyAlaThr145150155160SerGlyAspThrGlySerSerAlaGluTyrAlaMetArgGlyArgGlu165170175GlyIleArgValPheMetLeuThrProAlaGlyArgMetThrProPhe180185190GlnGlnAlaGlnMetPheGlyLeuAspAspProAsnIlePheAsnIle195200205AlaLeuAspGlyValPheAspAspCysGlnAspValValLysAlaVal210215220SerAlaAspAlaGluPheLysLysAspAsnArgIleGlyAlaValAsn225230235240SerIleAsnTrpAlaArgLeuMetAlaGlnValValTyrTyrValSer245250255SerTrpIleArgThrThrThrSerAsnAspGlnLysValSerPheSer260265270ValProThrGlyAsnPheGlyAspIleCysAlaGlyHisIleAlaArg275280285GlnMetGlyLeuProIleAspArgLeuIleValAlaThrAsnGluAsn290295300AspValLeuAspGluPhePheArgThrGlyAspTyrArgValArgSer305310315320SerAlaAspThrHisGluThrSerSerProSerMetAspIleSerArg325330335AlaSerAsnPheGluArgPheIlePheAspLeuLeuGlyArgAspAla340345350ThrArgValAsnAspLeuPheGlyThrGlnValArgGlnGlyGlyPhe355360365SerLeuAlaAspAspAlaAsnPheGluLysAlaAlaAlaGluTyrGly370375380PheAlaSerGlyArgSerThrHisAlaAspArgValAlaThrIleAla385390395400AspValHisSerArgLeuAspValLeuIleAspProHisThrAlaAsp405410415GlyValHisValAlaArgGlnTrpArgAspGluValAsnThrProIle420425430IleValLeuGluThrAlaLeuProValLysPheAlaAspThrIleVal435440445GluAlaIleGlyGluAlaProGlnThrProGluArgPheAlaAlaIle450455460MetAspAlaProPheLysValSerAspLeuProAsnAspThrAspAla465470475480ValLysGlnTyrIleValAspAlaIleAlaAsnThrSerValLys485490495<210>9<211>1092<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1092)<223>RXA00683W<400>9atgaccaacagtttgaacatcccgtttgtccagcgcttcgatgaaggc48MetThrAsnSerLeuAsnIleProPheValGlnArgPheAspGluGly151015ctggatcctgttctagaagtactcggtggcaagggcgcttcactagtc96LeuAspProValLeuGluValLeuGlyGlyLysGlyAlaSerLeuVal202530accatgacagatgctggaatgcccgttccacctggatttgtggtcact144ThrMetThrAspAlaGlyMetProValProProGlyPheValValThr354045actgccagctttgatgaattcatccgtgaagcaggggttgctgaacac192ThrAlaSerPheAspGluPheIleArgGluAlaGlyValAlaGluHis505560atcgataaattcctaaacgatctcgatgcagaagatgttaaggaagtg240IleAspLysPheLeuAsnAspLeuAspAlaGluAspValLysGluVal65707580gatcgagtttctgcgatcatccgcgatgagctgtgcagtcttgacgtt288AspArgValSerAlaIleIleArgAspGluLeuCysSerLeuAspVal859095ccagagaatgctcgtttcgcagtgcaccaggcttatcgcgatctcatg336ProGluAsnAlaArgPheAlaValHisGlnAlaTyrArgAspLeuMet100105110gaacgatgcggtggcgacgtcccggttgctgtccggtcatcggccact384GluArgCysGlyGlyAspValProValAlaValArgSerSerAlaThr115120125gccgaagatctgcccgatgcttccttcgcagggcaacaggacacctat432AlaGluAspLeuProAspAlaSerPheAlaGlyGlnGlnAspThrTyr130135140ctgtggcaagtcggtttgagcgctgtcactgaacacatccgtaaatgc480LeuTrpGlnValGlyLeuSerAlaValThrGluHisIleArgLysCys145150155160tgggcttcgctgttcacttcccgtgccattatctaccgtctgaaaaac528TrpAlaSerLeuPheThrSerArgAlaIleIleTyrArgLeuLysAsn165170175aacatccccaatgagggcctctccatggcggtagttgttcaaaaaatg576AsnIleProAsnGluGlyLeuSerMetAlaValValValGlnLysMet180185190gtcaactctcgtgtcgcaggcgtggcaatcactatgaatccttccaac624ValAsnSerArgValAlaGlyValAlaIleThrMetAsnProSerAsn195200205ggcgaccgctcgaagatcaccatcgattcctcatggggtgttggtgaa672GlyAspArgSerLysIleThrIleAspSerSerTrpGlyValGlyGlu210215220atggtggtctcaggtgaagtgacaccagacaatatcttgctggacaag720MetValValSerGlyGluValThrProAspAsnIleLeuLeuAspLys225230235240atcacgctgcaggttgtctccgaacacattggaagcaaacacgctgaa768IleThrLeuGlnValValSerGluHisIleGlySerLysHisAlaGlu245250255ctcatccccgatgccaccagtggaagcctcgtggaaaagcccgttgat816LeuIleProAspAlaThrSerGlySerLeuValGluLysProValAsp260265270gaagaacgcgcaaaccgccgcagtctgactgatgaggaaatgctcgct864GluGluArgAlaAsnArgArgSerLeuThrAspGluGluMetLeuAla275280285gtggcacaaatggctaagcgtgcagaaaaacactacaagtgcccacaa912ValAlaGlnMetAlaLysArgAlaGluLysHisTyrLysCysProGln290295300gatatcgaatgggcgctggacgctgatctgccagatggagaaaacctt960AspIleGluTrpAlaLeuAspAlaAspLeuProAspGlyGluAsnLeu305310315320ctgttattgcaatcccgcccggaaactatccactccaacggtgtgaag1008LeuLeuLeuGlnSerArgProGluThrIleHisSerAsnGlyValLys325330335aaggaaaccccaactccgcaggctgccaaaaccataggcaccttcgat1056LysGluThrProThrProGlnAlaAlaLysThrIleGlyThrPheAsp340345350ttcagctcaatcaccgtcgcaatgaccggcacgaag1092PheSerSerIleThrValAlaMetThrGlyThrLys355360<210>10<211>364<212>PRT<213>谷氨酸棒杆菌<400>10MetThrAsnSerLeuAsnIleProPheValGlnArgPheAspGluGly151015LeuAspProValLeuGluValLeuGlyGlyLysGlyAlaSerLeuVal202530ThrMetThrAspAlaGlyMetProValProProGlyPheValValThr354045ThrAlaSerPheAspGluPheIleArgGluAlaGlyValAlaGluHis505560IleAspLysPheLeuAsnAspLeuAspAlaGluAspValLysGluVal65707580AspArgValSerAlaIleIleArgAspGluLeuCysSerLeuAspVal859095ProGluAsnAlaArgPheAlaValHisGlnAlaTyrArgAspLeuMet100105110GluArgCysGlyGlyAspValProValAlaValArgSerSerAlaThr115120125AlaGluAspLeuProAspAlaSerPheAlaGlyGlnGlnAspThrTyr130135140LeuTrpGlnValGlyLeuSerAlaValThrGluHisIleArgLysCys145150155160TrpAlaSerLeuPheThrSerArgAlaIleIleTyrArgLeuLysAsn165170175AsnIleProAsnGluGlyLeuSerMetAlaValValValGlnLysMet180185190ValAsnSerArgValAlaGlyValAlaIleThrMetAsnProSerAsn195200205GlyAspArgSerLysIleThrIleAspSerSerTrpGlyValGlyGlu210215220MetValValSerGlyGluValThrProAspAsnIleLeuLeuAspLys225230235240IleThrLeuGlnValValSerGluHisIleGlySerLysHisAlaGlu245250255LeuIleProAspAlaThrSerGlySerLeuValGluLysProValAsp260265270GluGluArgAlaAsnArgArgSerLeuThrAspGluGluMetLeuAla275280285ValAlaGlnMetAlaLysArgAlaGluLysHisTyrLysCysProGln290295300AspIleGluTrpAlaLeuAspAlaAspLeuProAspGlyGluAsnLeu305310315320LeuLeuLeuGlnSerArgProGluThrIleHisSerAsnGlyValLys325330335LysGluThrProThrProGlnAlaAlaLysThrIleGlyThrPheAsp340345350PheSerSerIleThrValAlaMetThrGlyThrLys355360<210>11<211>1272<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1272)<223>RXA00783E<400>11ttgaaacacttacttttacgggaagactttgttaaagacgcagaaggc48MetLysHisLeuLeuLeuArgGluAspPheValLysAspAlaGluGly151015tctaagcatgggccggaaatggaattggcagtggatctttttgaatac96SerLysHisGlyProGluMetGluLeuAlaValAspLeuPheGluTyr202530caagcacgggacctctttgaaacccatggtgtgccagtgttgaaggga144GlnAlaArgAspLeuPheGluThrHisGlyValProValLeuLysGly354045attgtggcatcaacaccagaggcggcgaggaaagcggctgaggaaatc192IleValAlaSerThrProGluAlaAlaArgLysAlaAlaGluGluIle505560ggcggactgaccgtcgtcaaggctcaggtcaaggtgggcggacgtggc240GlyGlyLeuThrValValLysAlaGlnValLysValGlyGlyArgGly65707580aaggcgggtggcgtccgtgtggcaccgacgtcggctcaggcttttgat288LysAlaGlyGlyValArgValAlaProThrSerAlaGlnAlaPheAsp859095gctgcggatgcgattctcggcatggatatcaaaggacacactgttaat336AlaAlaAspAlaIleLeuGlyMetAspIleLysGlyHisThrValAsn100105110caggtgatggtggcgcagggcgctgacattgctgaggaatactatttc384GlnValMetValAlaGlnGlyAlaAspIleAlaGluGluTyrTyrPhe115120125tccattttgttggatcgcgcgaatcgttcgtatctggctatgtgctct432SerIleLeuLeuAspArgAlaAsnArgSerTyrLeuAlaMetCysSer130135140gttgaaggtggcatggagatcgagatcctggcgaaggaaaagcctgaa480ValGluGlyGlyMetGluIleGluIleLeuAlaLysGluLysProGlu145150155160gctttggcaaaggtggaagtggatcccctcactggtattgatgaggac528AlaLeuAlaLysValGluValAspProLeuThrGlyIleAspGluAsp165170175aaagcgcgggagattgtcactgctgctggctttgaaactgaggtggca576LysAlaArgGluIleValThrAlaAlaGlyPheGluThrGluValAla180185190gagaaagtcattccggtgctgatcaagatctggcaggtgtattacgaa624GluLysValIleProValLeuIleLysIleTrpGlnValTyrTyrGlu195200205gaggaagcaacactcgttgaggtgaacccgttggtgctcacggatgac672GluGluAlaThrLeuValGluValAsnProLeuValLeuThrAspAsp210215220ggcgatgtgattgcgcttgatggcaagatcacgctggatgataacgct720GlyAspValIleAlaLeuAspGlyLysIleThrLeuAspAspAsnAla225230235240gatttccgccatgataaccgtggtgcgttggctgaatctgccggtggc768AspPheArgHisAspAsnArgGlyAlaLeuAlaGluSerAlaGlyGly245250255ttggacattttggaactgaaggccaagaagaatgatctgaactacgtg816LeuAspIleLeuGluLeuLysAlaLysLysAsnAspLeuAsnTyrVal260265270aaacttgatggctctgtgggcatcattggcaatggtgcaggtttggtg864LysLeuAspGlySerValGlyIleIleGlyAsnGlyAlaGlyLeuVal275280285atgtccacgttggatatcgtggctgcagctggtgaacgccatggtggg912MetSerThrLeuAspIleValAlaAlaAlaGlyGluArgHisGlyGly290295300cagcgccccgcgaacttcctagacattggtggcggagcatcagctgaa960GlnArgProAlaAsnPheLeuAspIleGlyGlyGlyAlaSerAlaGlu305310315320tcgatggctgctggtctcgatgtgatccttggggatagccaggtacgc1008SerMetAlaAlaGlyLeuAspValIleLeuGlyAspSerGlnValArg325330335agtgtgtttgtgaatgtgtttggtggcatcaccgcgtgtgatgtggtg1056SerValPheValAsnValPheGlyGlyIleThrAlaCysAspValVal340345350gcaaagggaatcgttggagctttggatgtgctcggcgatcaagcaacg1104AlaLysGlyIleValGlyAlaLeuAspValLeuGlyAspGlnAlaThr355360365aagcctcttgtggtgcgccttgatggcaacaacgtggtggaaggcaga1152LysProLeuValValArgLeuAspGlyAsnAsnValValGluGlyArg370375380cgaatcctcgcggaatataaccaccctttggtcaccgttgtggagggt1200ArgIleLeuAlaGluTyrAsnHisProLeuValThrValValGluGly385390395400atggatgcagcggctgatcacgctgcccatttggccaatcttgcccag1248MetAspAlaAlaAlaAspHisAlaAlaHisLeuAlaAsnLeuAlaGln405410415cacggccagttcgcaaccgctaat1272HisGlyGlnPheAlaThrAlaAsn420<210>12<211>424<212>PRT<213>谷氨酸棒杆菌<400>12MetLysHisLeuLeuLeuArgGluAspPheValLysAspAlaGluGly151015SerLysHisGlyProGluMetGluLeuAlaValAspLeuPheGluTyr202530GlnAlaArgAspLeuPheGluThrHisGlyValProValLeuLysGly354045IleValAlaSerThrProGluAlaAlaArgLysAlaAlaGluGluIle505560GlyGlyLeuThrValValLysAlaGlnValLysValGlyGlyArgGly65707580LysAlaGlyGlyValArgValAlaProThrSerAlaGlnAlaPheAsp859095AlaAlaAspAlaIleLeuGlyMetAspIleLysGlyHisThrValAsn100105110GlnValMetValAlaGlnGlyAlaAspIleAlaGluGluTyrTyrPhe115120125SerIleLeuLeuAspArgAlaAsnArgSerTyrLeuAlaMetCysSer130135140ValGluGlyGlyMetGluIleGluIleLeuAlaLysGluLysProGlu145150155160AlaLeuAlaLysValGluValAspProLeuThrGlyIleAspGluAsp165170175LysAlaArgGluIleValThrAlaAlaGlyPheGluThrGluValAla180185190GluLysValIleProValLeuIleLysIleTrpGlnValTyrTyrGlu195200205GluGluAlaThrLeuValGluValAsnProLeuValLeuThrAspAsp210215220GlyAspValIleAlaLeuAspGlyLysIleThrLeuAspAspAsnAla225230235240AspPheArgHisAspAsnArgGlyAlaLeuAlaGluSerAlaGlyGly245250255LeuAspIleLeuGluLeuLysAlaLysLysAsnAspLeuAsnTyrVal260265270LysLeuAspGlySerValGlyIleIleGlyAsnGlyAlaGlyLeuVal275280285MetSerThrLeuAspIleValAlaAlaAlaGlyGluArgHisGlyGly290295300GlnArgProAlaAsnPheLeuAspIleGlyGlyGlyAlaSerAlaGlu305310315320SerMetAlaAlaGlyLeuAspValIleLeuGlyAspSerGlnValArg325330335SerValPheValAsnValPheGlyGlyIleThrAlaCysAspValVal340345350AlaLysGlyIleValGlyAlaLeuAspValLeuGlyAspGlnAlaThr355360365LysProLeuValValArgLeuAspGlyAsnAsnValValGluGlyArg370375380ArgIleLeuAlaGluTyrAsnHisProLeuValThrValValGluGly385390395400MetAspAlaAlaAlaAspHisAlaAlaHisLeuAlaAsnLeuAlaGln405410415HisGlyGlnPheAlaThrAlaAsn420<210>l3<2ll>1194<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1194)<223>RXA00783K<400>13gtggatctttttgaataccaagcacgggacctctttgaaacccatggt48MetAspLeuPheGluTyrGlnAlaArgAspLeuPheGluThrHisGlyl51015gtgccagtgttgaagggaattgtggcatcaacaccagaggcggcgagg96ValProValLeuLysGlyIleValAlaSerThrProGluAlaAlaArg202530aaagcggctgaggaaatcggcggactgaccgtcgtcaaggctcaggtc144LysAlaAlaGluGluIleGlyGlyLeuThrValValLysAlaGlnVal354045aaggtgggcggacgtggcaaggcgggtggcgtccgtgtggcaccgacg192LysValGlyGlyArgGlyLysAlaGlyGlyValArgValAlaProThr505560tcggctcaggcttttgatgctgcggatgcgattctcggcatggatatc240SerAlaGlnAlaPheAspAlaAlaAspAlaIleLeuGlyMetAspIle65707580aaaggacacactgttaatcaggtgatggtggcgcagggcgctgacatt288LysGlyHisThrValAsnGlnValMetValAlaGlnGlyAlaAspIle859095gctgaggaatactatttctccattttgttggatcgcgcgaatcgttcg336AlaGluGluTyrTyrPheSerIleLeuLeuAspArgAlaAsnArgSer100105110tatctggctatgtgctctgttgaaggtggcatggagatcgagatcctg384TyrLeuAlaMetCysSerValGluGlyGlyMetGluIleGluIleLeu115120125gcgaaggaaaagcctgaagctttggcaaaggtggaagtggatcccctc432AlaLysGluLysProGluAlaLeuAlaLysValGluValAspProLeu130135140actggtattgatgaggacaaagcgcgggagattgtcactgctgctggc480ThrGlyIleAspGluAspLysAlaArgGluIleValThrAlaAlaGly145150155160tttgaaactgaggtggcagagaaagtcattccggtgctgatcaagatc528PheGluThrGluValAlaGluLysValIleProValLeuIleLysIle165170175tggcaggtgtattacgaagaggaagcaacactcgttgaggtgaacccg576TrpGlnValTyrTyrGluGluGluAlaThrLeuValGluValAsnPro180185190ttggtgctcacggatgacggcgatgtgattgcgcttgatggcaagatc624LeuValLeuThrAspAspGlyAspValIleAlaLeuAspGlyLysIle195200205acgctggatgataacgctgatttccgccatgataaccgtggtgcgttg672ThrLeuAspAspAsnAlaAspPheArgHisAspAsnArgGlyAlaLeu210215220gctgaatctgccggtggcttggacattttggaactgaaggccaagaag720AlaGluSerAlaGlyGlyLeuAspIleLeuGluLeuLysAlaLysLys225230235240aatgatctgaactacgtgaaacttgatggctctgtgggcatcattggc768AsnAspLeuAsnTyrValLysLeuAspGlySerValGlyIleIleGly245250255aatggtgcaggtttggtgatgtccacgttggatatcgtggctgcagct816AsnGlyAlaGlyLeuValMetSerThrLeuAspIleValAlaAlaAla260265270ggtgaacgccatggtgggcagcgccccgcgaacttcctagacattggt864GlyGluArgHisGlyGlyGlnArgProAlaAsnPheLeuAspIleGly275280285ggcggagcatcagctgaatcgatggctgctggtctcgatgtgatcctt912GlyGlyAlaSerAlaGluSerMetAlaAlaGlyLeuAspValIleLeu290295300ggggatagccaggtacgcagtgtgtttgtgaatgtgtttggtggcatc960GlyAspSerGlnValArgSerValPheValAsnValPheGlyGlyIle305310315320accgcgtgtgatgtggtggcaaagggaatcgttggagctttggatgtg1008ThrAlaCysAspValValAlaLysGlyIleValGlyAlaLeuAspVal325330335ctcggcgatcaagcaacgaagcctcttgtggtgcgccttgatggcaac1056LeuGlyAspGlnAlaThrLysProLeuValValArgLeuAspGlyAsn340345350aacgtggtggaaggcagacgaatcctcgcggaatataaccaccctttg1104AsnValValGluGlyArgArgIleLeuAlaGluTyrAsnHisProLeu355360365gtcaccgttgtggagggtatggatgcagcggctgatcacgctgcccat1152ValThrValValGluGlyMetAspAlaAlaAlaAspHisAlaAlaHis370375380ttggccaatcttgcccagcacggccagttcgcaaccgctaat1194LeuAlaAsnLeuAlaGlnHisGlyGlnPheAlaThrAlaAsn385390395<210>14<211>398<212>PRT<213>谷氨酸棒杆菌<400>14MetAspLeuPheGluTyrGlnAlaArgAspLeuPheGluThrHisGly151015ValProValLeuLysGlyIleValAlaSerThrProGluAlaAlaArg202530LysAlaAlaGluGluIleGlyGlyLeuThrValValLysAlaGlnVal354045LysValGlyGlyArgGlyLysAlaGlyGlyValArgValAlaProThr505560SerAlaGlnAlaPheAspAlaAlaAspAlaIleLeuGlyMetAspIle65707580LysGlyHisThrValAsnGlnValMetValAlaGlnGlyAlaAspIle859095AlaGluGluTyrTyrPheSerIleLeuLeuAspArgAlaAsnArgSer100105110TyrLeuAlaMetCysSerValGluGlyGlyMetGluIleGluIleLeu115120125AlaLysGluLysProGluAlaLeuAlaLysValGluValAspProLeu130135140ThrGlyIleAspGluAspLysAlaArgGluIleValThrAlaAlaGly145150155160PheGluThrGluValAlaGluLysValIleProValLeuIleLysIle165170175TrpGlnValTyrTyrGluGluGluAlaThrLeuValGluValAsnPro180185190LeuValLeuThrAspAspGlyAspValIleAlaLeuAspGlyLysIle195200205ThrLeuAspAspAsnAlaAspPheArgHisAspAsnArgGlyAlaLeu210215220AlaGluSerAlaGlyGlyLeuAspIleLeuGluLeuLysAlaLysLys225230235240AsnAspLeuAsnTyrValLysLeuAspGlySerValGlyIleIleGly245250255AsnGlyAlaGlyLeuValMetSerThrLeuAspIleValAlaAlaAla260265270GlyGluArgHisGlyGlyGlnArgProAlaAsnPheLeuAspIleGly275280285GlyGlyAlaSerAlaGluSerMetAlaAlaGlyLeuAspValIleLeu290295300GlyAspSerGlnValArgSerValPheValAsnValPheGlyGlyIle305310315320ThrAlaCysAspValValAlaLysGlyIleValGlyAlaLeuAspVal325330335LeuGlyAspGlnAlaThrLysProLeuValValArgLeuAspGlyAsn340345350AsnValValGluGlyArgArgIleLeuAlaGluTyrAsnHisProLeu355360365ValThrValValGluGlyMetAspAlaAlaAlaAspHisAlaAlaHis370375380LeuAlaAsnLeuAlaGlnHisGlyGlnPheAlaThrAlaAsn385390395<210>15<211>1335<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1335)<223>RXA00969K<400>15atgacctcagcatctgccccaagctttaaccccggcaagggtcccggc48MetThrSerAlaSerAlaProSerPheAsnProGlyLysGlyProGly151015tcagcagtcggaattgcccttttaggattcggaacagtcggcactgag96SerAlaValGlyIleAlaLeuLeuGlyPheGlyThrValGlyThrGlu202530gtgatgcgtctgatgaccgagtacggtgatgaacttgcgcaccgcatt144ValMetArgLeuMetThrGluTyrGlyAspGluLeuAlaHisArgIle354045ggtggcccactggaggttcgtggcattgctgtttctgatatctcaaag192GlyGlyProLeuGluValArgGlyIleAlaValSerAspIleSerLys505560ccacgtgaaggcgttgcacctgagctgctcactgaggacgcttttgca240ProArgGluGlyValAlaProGluLeuLeuThrGluAspAlaPheAla65707580ctcatcgagcgcgaggatgttgacatcgtcgttgaggttatcggcggc288LeuIleGluArgGluAspValAspIleValValGluValIleGlyGly859095attgagtacccacgtgaggtagttctcgcagctctgaaggccggcaag336IleGluTyrProArgGluValValLeuAlaAlaLeuLysAlaGlyLys100105110tctgttgttaccgccaataaggctcttgttgcagctcactctgctgag384SerValValThrAlaAsnLysAlaLeuValAlaAlaHisSerAlaGlu115120125cttgctgatgcagcggaagccgcaaacgttgacctgtacttcgaggct432LeuAlaAspAlaAlaGluAlaAlaAsnValAspLeuTyrPheGluAla130135140gctgttgcaggcgcaattccagtggttggcccactgcgtcgctccctg480AlaValAlaGlyAlaIleProValValGlyProLeuArgArgSerLeu145150155160gctggcgatcagatccagtctgtgatgggcatcgttaacggcaccacc528AlaGlyAspGlnIleGlnSerValMetGlyIleValAsnGlyThrThr165170175aacttcatcttggacgccatggattccaccggcgctgactatgcagat576AsnPheIleLeuAspAlaMetAspSerThrGlyAlaAspTyrAlaAsp180185190tctttggctgaggcaactcgtttgggttacgccgaagctgatccaact624SerLeuAlaGluAlaThrArgLeuGlyTyrAlaGluAlaAspProThr195200205gcagacgtcgaaggccatgacgccgcatccaaggctgcaattttggca672AlaAspValGluGlyHisAspAlaAlaSerLysAlaAlaIleLeuAla210215220tccatcgctttccacacccgtgttaccgcggatgatgtgtactgcgaa720SerIleAlaPheHisThrArgValThrAlaAspAspValTyrCysGlu225230235240ggtatcagcaacatcagcgctgccgacattgaggcagcacagcaggca768GlyIleSerAsnIleSerAlaAlaAspIleGluAlaAlaGlnGlnAla245250255ggccacaccatcaagttgttggccatctgtgagaagttcaccaacaag816GlyHisThrIleLysLeuLeuAlaIleCysGluLysPheThrAsnLys260265270gaaggaaagtcggctatttctgctcgcgtgcacccgactctattacct864GluGlyLysSerAlaIleSerAlaArgValHisProThrLeuLeuPro275280285gtgtcccacccactggcgtcggtaaacaagtcctttaatgcaatcttt912ValSerHisProLeuAlaSerValAsnLysSerPheAsnAlaIlePhe290295300gttgaagcagaagcagctggtcgcctgatgttctacggaaacggtgca960ValGluAlaGluAlaAlaGlyArgLeuMetPheTyrGlyAsnGlyAla305310315320ggtggcgcgccaaccgcgtctgctgtgcttggcgacgtcgttggtgcc1008GlyGlyAlaProThrAlaSerAlaValLeuGlyAspValValGlyAla325330335gcacgaaacaaggtgcacggtggccgtgctccaggtgagtccacctac1056AlaArgAsnLysValHisGlyGlyArgAlaProGlyGluSerThrTyr340345350gctaacctgccgatcgctgatttcggtgagaccaccactcgttaccac1104AlaAsnLeuProIleAlaAspPheGlyGluThrThrThrArgTyrHis355360365ctcgacatggatgtggaagatcgcgtgggggttttggctgaattggct1152LeuAspMetAspValGluAspArgValGlyValLeuAlaGluLeuAla370375380agcctgttctctgagcaaggaatctccctgcgtacaatccgacaggaa1200SerLeuPheSerGluGlnGlyIleSerLeuArgThrIleArgGlnGlu385390395400gagcgcgatgatgatgcacgtctgatcgtggtcacccactctgcgctg1248GluArgAspAspAspAlaArgLeuIleValValThrHisSerAlaLeu405410415gaatctgatctttcccgcaccgttgaactgctgaaggctaagcctgtt1296GluSerAspLeuSerArgThrValGluLeuLeuLysAlaLysProVal420425430gttaaggcaatcaacagtgtgatccgcctcgaaagggac1335ValLysAlaIleAsnSerValIleArgLeuGluArgAsp435440445<210>16<211>445<212>PRT<213>谷氨酸棒杆菌<400>16MetThrSerAlaSerAlaProSerPheAsnProGlyLysGlyProGly151015SerAlaValGlyIleAlaLeuLeuGlyPheGlyThrValGlyThrGlu202530ValMetArgLeuMetThrGluTyrGlyAspGluLeuAlaHisArgIle354045GlyGlyProLeuGluValArgGlyIleAlaValSerAspIleSerLys505560ProArgGluGlyValAlaProGluLeuLeuThrGluAspAlaPheAla65707580LeuIleGluArgGluAspValAspIleValValGluValIleGlyGly859095IleGluTyrProArgGluValValLeuAlaAlaLeuLysAlaGlyLys100105110SerValValThrAlaAsnLysAlaLeuValAlaAlaHisSerAlaGlu115120125LeuAlaAspAlaAlaGluAlaAlaAsnValAspLeuTyrPheGluAla130135140AlaValAlaGlyAlaIleProValValGlyProLeuArgArgSerLeu145150155160AlaGlyAspGlnIleGlnSerValMetGlyIleValAsnGlyThrThr165170175AsnPheIleLeuAspAlaMetAspSerThrGlyAlaAspTyrAlaAsp180185190SerLeuAlaGluAlaThrArgLeuGlyTyrAlaGluAlaAspProThr195200205AlaAspValGluGlyHisAspAlaAlaSerLysAlaAlaIleLeuAla210215220SerIleAlaPheHisThrArgValThrAlaAspAspValTyrCysGlu225230235240GlyIleSerAsnIleSerAlaAlaAspIleGluAlaAlaGlnGlnAla245250255GlyHisThrIleLysLeuLeuAlaIleCysGluLysPheThrAsnLys260265270GluGlyLysSerAlaIleSerAlaArgValHisProThrLeuLeuPro275280285ValSerHisProLeuAlaSerValAsnLysSerPheAsnAlaIlePhe290295300ValGluAlaGluAlaAlaGlyArgLeuMetPheTyrGlyAsnGlyAla305310315320GlyGlyAlaProThrAlaSerAlaValLeuGlyAspValValGlyAla325330335AlaArgAsnLysValHisGlyGlyArgAlaProGlyGluSerThrTyr340345350AlaAsnLeuProIleAlaAspPheGlyGluThrThrThrArgTyrHis355360365LeuAspMetAspValGluAspArgValGlyValLeuAlaGluLeuAla370375380SerLeuPheSerGluGlnGlyIleSerLeuArgThrIleArgGlnGlu385390395400GluArgAspAspAspAlaArgLeuIleValValThrHisSerAlaLeu405410415GluSerAspLeuSerArgThrValGluLeuLeuLysAlaLysProVal420425430ValLysAlaIleAsnSerValIleArgLeuGluArgAsp435440445<210>17<211>690<212>DNA<213>谷氨酸棒杆菌<220><22l>CDS<222>(1)..(690)<223>RCGL01443K<400>17gtgaccacacaaaccgcccctaccgcagcctccgatctttttgccgag48MetThrThrGlnThrAlaProThrAlaAlaSerAspLeuPheAlaGlu151015tcctacaaactcagcccaaagcagcgcgaagttttagatgccctacaa96SerTyrLysLeuSerProLysGlnArgGluValLeuAspAlaLeuGln202530accttccctgacggtgcccgcgcgatcgatgtcgccaaaaaactcgac144ThrPheProAspGlyAlaArgAlaIleAspValAlaLysLysLeuAsp354045cttcacgtcaacaccgcccgcggccacctcgaagaactcgtagcaaaa192LeuHisValAsnThrAlaArgGlyHisLeuGluGluLeuValAlaLys505560gaagccatccgagtagtcaccgcagcagccaaaggtcgcggacgcccc240GluAlaIleArgValValThrAlaAlaAlaLysGlyArgGlyArgPro65707580tccctcatcttccaaacacgcgtccccgacaaccgcgctgtcgccaaa288SerLeuIlePheGlnThrArgValProAspAsnArgAlaValAlaLys859095gaatacatcaccctgatcgaactcatggccaacatgctcggcgatgtc336GluTyrIleThrLeuIleGluLeuMetAlaAsnMetLeuGlyAspVal100105110gacgatgacgcaatgcacaaccccgacctccgcgccaaagcactttcc384AspAspAspAlaMetHisAsnProAspLeuArgAlaLysAlaLeuSer115120125atcggaacccagtgggcacacgtcatgggcattaaccacgccgaagcc432IleGlyThrGlnTrpAlaHisValMetGlyIleAsnHisAlaGluAla130135140gaagaactcgacgaagccctctccccgctcattaaccgcctccgcgaa480GluGluLeuAspGluAlaLeuSerProLeuIleAsnArgLeuArgGlu145150155160atgggctttgaccccaccgaaaccgaagaagcaaactccctcgctcta528MetGlyPheAspProThrGluThrGluGluAlaAsnSerLeuAlaLeu165170175cacagctgcccatttgtggtcaacgacaaacgcccatcagccttcgtc576HisSerCysProPheValValAsnAspLysArgProSerAlaPheVal180185190tgcgccatccacgccggattcatccaagaaagcctcggtgaaaacaac624CysAlaIleHisAlaGlyPheIleGlnGluSerLeuGlyGluAsnAsn195200205cgcatccagctggaactcaaaccactcaacgcgccgggcacctgtaag672ArgIleGlnLeuGluLeuLysProLeuAsnAlaProGlyThrCysLys210215220gttcacgtgttcagcgaa690ValHisValPheSerGlu225230<210>18<211>230<212>PRT<213>谷氨酸棒杆菌<400>18MetThrThrGlnThrAlaProThrAlaAlaSerAspLeuPheAlaGlu151015SerTyrLysLeuSerProLysGlnArgGluValLeuAspAlaLeuGln202530ThrPheProAspGlyAlaArgAlaIleAspValAlaLysLysLeuAsp354045LeuHisValAsnThrAlaArgGlyHisLeuGluGluLeuValAlaLys505560GluAlaIleArgValValThrAlaAlaAlaLysGlyArgGlyArgPro65707580SerLeuIlePheGlnThrArgValProAspAsnArgAlaValAlaLys859095GluTyrIleThrLeuIleGluLeuMetAlaAsnMetLeuGlyAspVal100105110AspAspAspAlaMetHisAsnProAspLeuArgAlaLysAlaLeuSer115120125IleGlyThrGlnTrpAlaHisValMetGlyIleAsnHisAlaGluAla130135140GluGluLeuAspGluAlaLeuSerProLeuIleAsnArgLeuArgGlu145150155160MetGlyPheAspProThrGluThrGluGluAlaAsnSerLeuAlaLeu165170175HisSerCysProPheValValAsnAspLysArgProSerAlaPheVal180185190CysAlaIleHisAlaGlyPheIleGlnGluSerLeuGlyGluAsnAsn195200205ArgIleGlnLeuGluLeuLysProLeuAsnAlaProGlyThrCysLys210215220ValHisValPheSerGlu225230<210>19<211>1476<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1476)<223>RXA00999K<400>19atgccgtcaagtacgatcaataacatgactaatggagataatctcgca48MetProSerSerThrIleAsnAsnMetThrAsnGlyAspAsnLeuAla151015cagatcggcgttgtaggcctagcagtaatgggctcaaacctcgcccgc96GlnIleGlyValValGlyLeuAlaValMetGlySerAsnLeuAlaArg202530aacttcgcccgcaacggcaacactgtcgctgtctacaaccgcagcact144AsnPheAlaArgAsnGlyAsnThrValAlaValTyrAsnArgSerThr354045gacaaaaccgacaagctcatcgccgatcacggctccgaaggcaacttc192AspLysThrAspLysLeuIleAlaAspHisGlySerGluGlyAsnPhe505560atcccttctgcaaccgtcgaagagttcgtagcatccctggaaaagcca240IleProSerAlaThrValGluGluPheValAlaSerLeuGluLysPro65707580cgccgcgccatcatcatggttcaggctggtaacgccaccgacgcagtc288ArgArgAlaIleIleMetValGlnAlaGlyAsnAlaThrAspAlaVal859095atcaaccagctggcagatgccatggacgaaggcgacatcatcatcgac336IleAsnGlnLeuAlaAspAlaMetAspGluGlyAspIleIleIleAsp100105110ggcggcaacgccctctacaccgacaccattcgtcgcgagaaggaaatc384GlyGlyAsnAlaLeuTyrThrAspThrIleArgArgGluLysGluIle115120125tccgcacgcggtctccacttcgtcggtgctggtatctccggcggcgaa432SerAlaArgGlyLeuHisPheValGlyAlaGlyIleSerGlyGlyGlu130135140gaaggcgcactcaacggcccatccatcatgcctggtggcccagcaaag480GluGlyAlaLeuAsnGlyProSerIleMetProGlyGlyProAlaLys145150155160tcctacgagtccctcggaccactgcttgagtccatcgctgccaacgtt528SerTyrGluSerLeuGlyProLeuLeuGluSerIleAlaAlaAsnVal165170175gacggcaccccatgtgtcacccacatcggcccagacggcgccggccac576AspGlyThrProCysValThrHisIleGlyProAspGlyAlaGlyHis180185190ttcgtcaagatggtccacaacggcatcgagtacgccgacatgcaggtc624PheValLysMetValHisAsnGlyIleGluTyrAlaAspMetGlnVal195200205atcggcgaggcataccaccttctccgctacgcagcaggcatgcagcca672IleGlyGluAlaTyrHisLeuLeuArgTyrAlaAlaGlyMetGlnPro210215220gctgaaatcgctgaggttttcaaggaatggaacgcaggcgacctggat720AlaGluIleAlaGluValPheLysGluTrpAsnAlaGlyAspLeuAsp225230235240tcctacctcatcgaaatcaccgcagaggttctctcccaggtggatgct768SerTyrLeuIleGluIleThrAlaGluValLeuSerGlnValAspAla245250255gaaaccggcaagccactaatcgacgtcatcgttgacgctgcaggtcag816GluThrGlyLysProLeuIleAspValIleValAspAlaAlaGlyGln260265270aagggcaccggacgttggaccgtcaaggctgctcttgatctgggtatt864LysGlyThrGlyArgTrpThrValLysAlaAlaLeuAspLeuGlyIle275280285gctaccaccggcatcggcgaagctgttttcgcacgtgcactctccggc912AlaThrThrGlyIleGlyGluAlaValPheAlaArgAlaLeuSerGly290295300gcaaccagccagcgcgctgcagcacagggcaacctacctgcaggtgtc960AlaThrSerGlnArgAlaAlaAlaGlnGlyAsnLeuProAlaGlyVal305310315320ctcaccgatctggaagcacttggcgtggacaaggcacagttcgtcgaa1008LeuThrAspLeuGluAlaLeuGlyValAspLysAlaGlnPheValGlu325330335gacgttcgccgtgcactgtacgcatccaagcttgttgcttacgcacag1056AspValArgArgAlaLeuTyrAlaSerLysLeuValAlaTyrAlaGln340345350ggcttcgacgagatcaaggctggctccgacgagaacaactgggacgtt1104GlyPheAspGluIleLysAlaGlySerAspGluAsnAsnTrpAspVal355360365gaccctcgcgacctcgctaccatctggcgcggcggctgcatcattcgc1152AspProArgAspLeuAlaThrIleTrpArgGlyGlyCysIleIleArg370375380gctaagttcctcaaccgcatcgtcgaagcatacgatgcaaacgctgaa1200AlaLysPheLeuAsnArgIleValGluAlaTyrAspAlaAsnAlaGlu385390395400cttgagtccctgctgctcgatccttacttcaagagcgagctcggcgac1248LeuGluSerLeuLeuLeuAspProTyrPheLysSerGluLeuGlyAsp405410415ctcatcgattcatggcgtcgcgtgattgtcaccgccacccagcttggc1296LeuIleAspSerTrpArgArgValIleValThrAlaThrGlnLeuGly420425430ctgccaatcccagtgttcgcttcctccctgtcctactacgacagcctg1344LeuProIleProValPheAlaSerSerLeuSerTyrTyrAspSerLeu435440445cgtgcagagcgtctgccagcagccctgatccaaggacagcgcgacttc1392ArgAlaGluArgLeuProAlaAlaLeuIleGlnGlyGlnArgAspPhe450455460ttcggtgcgcacacctacaagcgcatcgacaaggatggctccttccac1440PheGlyAlaHisThrTyrLysArgIleAspLysAspGlySerPheHis465470475480accgagtggtccggcgaccgctccgaggttgaagct1476ThrGluTrpSerGlyAspArgSerGluValGluAla485490<210>20<211>492<212>PRT<213>谷氨酸棒杆菌<400>20MetProSerSerThrIleAsnAsnMetThrAsnGlyAspAsnLeuAla151015GlnIleGlyValValGlyLeuAlaValMetGlySerAsnLeuAlaArg202530AsnPheAlaArgAsnGlyAsnThrValAlaValTyrAsnArgSerThr354045AspLysThrAspLysLeuIleAlaAspHisGlySerGluGlyAsnPhe505560IleProSerAlaThrValGluGluPheValAlaSerLeuGluLysPro65707580ArgArgAlaIleIleMetValGlnAlaGlyAsnAlaThrAspAlaVal859095IleAsnGlnLeuAlaAspAlaMetAspGluGlyAspIleIleIleAsp100105110GlyGlyAsnAlaLeuTyrThrAspThrIleArgArgGluLysGluIle115120125SerAlaArgGlyLeuHisPheValGlyAlaGlyIleSerGlyGlyGlu130135140GluGlyAlaLeuAsnGlyProSerIleMetProGlyGlyProAlaLys145150155160SerTyrGluSerLeuGlyProLeuLeuGluSerIleAlaAlaAsnVal165170175AspGlyThrProCysValThrHisIleGlyProAspGlyAlaGlyHis180185190PheValLysMetValHisAsnGlyIleGluTyrAlaAspMetGlnVal195200205IleGlyGluAlaTyrHisLeuLeuArgTyrAlaAlaGlyMetGlnPro210215220AlaGluIleAlaGluValPheLysGluTrpAsnAlaGlyAspLeuAsp225230235240SerTyrLeuIleGluIleThrAlaGluValLeuSerGlnValAspAla245250255GluThrGlyLysProLeuIleAspValIleValAspAlaAlaGlyGln260265270LysGlyThrGlyArgTrpThrValLysAlaAlaLeuAspLeuGlyIle275280285AlaThrThrGlyIleGlyGluAlaValPheAlaArgAlaLeuSerGly290295300AlaThrSerGlnArgAlaAlaAlaGlnGlyAsnLeuProAlaGlyVal305310315320LeuThrAspLeuGluAlaLeuGlyValAspLysAlaGlnPheValGlu325330335AspValArgArgAlaLeuTyrAlaSerLysLeuValAlaTyrAlaGln340345350GlyPheAspGluIleLysAlaGlySerAspGluAsnAsnTrpAspVal355360365AspProArgAspLeuAlaThrIleTrpArgGlyGlyCysIleIleArg370375380AlaLysPheLeuAsnArgIleValGluAlaTyrAspAlaAsnAlaGlu385390395400LeuGluSerLeuLeuLeuAspProTyrPheLysSerGluLeuGlyAsp405410415LeuIleAspSerTrpArgArgValIleValThrAlaThrGlnLeuGly420425430LeuProIleProValPheAlaSerSerLeuSerTyrTyrAspSerLeu435440445ArgAlaGluArgLeuProAlaAlaLeuIleGlnGlyGlnArgAspPhe450455460PheGlyAlaHisThrTyrLysArgIleAspLysAspGlySerPheHis465470475480ThrGluTrpSerGlyAspArgSerGluValGluAla485490<210>21<211>1431<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1431)<223>RXA0l093W<400>21atgggcgtggatagacgaactaagattgtatgtaccctaggcccagcg48MetGlyValAspArgArgThrLysIleValCysThrLeuGlyProAla151015gtggctagtgcagatggaattctgcgtttggtagaagacggcatggat96ValAlaSerAlaAspGlyIleLeuArgLeuValGluAspGlyMetAsp202530gttgctcgcctcaacttctcccatggtgaccacccagatcatgagcaa144ValAlaArgLeuAsnPheSerHisGlyAspHisProAspHisGluGln354045aactacaagtgggtccgcgaggcggcggagaagactggccgtgcagtc192AsnTyrLysTrpValArgGluAlaAlaGluLysThrGlyArgAlaVal505560ggtattctcgcagacctccaaggaccgaagatccgtcttggccgtttc240GlyIleLeuAlaAspLeuGlnGlyProLysIleArgLeuGlyArgPhe65707580actgacggcgcaaccgtgtgggaaaacggcgagaccattcggatcacc288ThrAspGlyAlaThrValTrpGluAsnGlyGluThrIleArgIleThr859095gttgacgatgtagagggaacgcacgatcgtgtgtccaccacctacaag336ValAspAspValGluGlyThrHisAspArgValSerThrThrTyrLys100105110aatctggcaaaagacgcgaagccaggcgaccgcctgctcgttgatgac384AsnLeuAlaLysAspAlaLysProGlyAspArgLeuLeuValAspAsp115120125ggcaaggttggcctcgtctgcgtttccgtcgaaggtaacgacgtcatc432GlyLysValGlyLeuValCysValSerValGluGlyAsnAspValIle130135140tgtgaggttgttgagggcggaccagtctccaacaacaagggtgtttcc480CysGluValValGluGlyGlyProValSerAsnAsnLysGlyValSer145150155160ctgccaggtatggatatttccgtacctgcactgtccgaaaaggatatc528LeuProGlyMetAspIleSerValProAlaLeuSerGluLysAspIle165170175cgtgacctgcgcttcgccctgaagctcggcgtggactttattgcactg576ArgAspLeuArgPheAlaLeuLysLeuGlyValAspPheIleAlaLeu180185190tccttcgtacgttccccagcagatgctgaactcgttcacaagatcatg624SerPheValArgSerProAlaAspAlaGluLeuValHisLysIleMet195200205gacgaagaaggtcgtcgtgttcctgtgatcgccaagctggaaaagcca672AspGluGluGlyArgArgValProValIleAlaLysLeuGluLysPro210215220gaggctgtcacctccctcgagccaatcgtgttggcattcgacgccgtc720GluAlaValThrSerLeuGluProIleValLeuAlaPheAspAlaVal225230235240atggttgctcgtggtgacctcggcgttgaggttcctctggaggaggtt768MetValAlaArgGlyAspLeuGlyValGluValProLeuGluGluVal245250255ccactggttcagaagcgcgcaatccagattgcccgtgagaacgcaaag816ProLeuValGlnLysArgAlaIleGlnIleAlaArgGluAsnAlaLys260265270ccagttatcgtggcaacccagatgctggattccatgattgagaactcc864ProValIleValAlaThrGlnMetLeuAspSerMetIleGluAsnSer275280285cgcccaacccgtgcggaagcttctgacgtggcaaacgctgtgctcgat912ArgProThrArgAlaGluAlaSerAspValAlaAsnAlaValLeuAsp290295300ggcgcagatgctgtcatgctttctggtgaaacttcagtgggcaaagat960GlyAlaAspAlaValMetLeuSerGlyGluThrSerValGlyLysAsp305310315320ccgcacaacgttgtgcgcaccatgtctcgcattgttcgcttcgctgaa1008ProHisAsnValValArgThrMetSerArgIleValArgPheAlaGlu325330335accgacggtcgcgtcccagacctgacccacatccctcgcactaagcgt1056ThrAspGlyArgValProAspLeuThrHisIleProArgThrLysArg340345350ggcgttatttcctactctgcacgtgatatcgccgagcgcctcaacgct1104GlyValIleSerTyrSerAlaArgAspIleAlaGluArgLeuAsnAla355360365cgtgcattggttgcgttcaccacctctggtgataccgcaaagcgtgtg1152ArgAlaLeuValAlaPheThrThrSerGlyAspThrAlaLysArgVal370375380gctcgtctgcacagccacctgccactgctcgtgttcactccaaatgag1200AlaArgLeuHisSerHisLeuProLeuLeuValPheThrProAsnGlu385390395400gcagttcgctctgagctggcgctgacctggggtgcaaccaccttcctg1248AlaValArgSerGluLeuAlaLeuThrTrpGlyAlaThrThrPheLeu405410415tgtccacctgtcagcgataccgatgacatgatgcgcgaagtcgaccgt1296CysProProValSerAspThrAspAspMetMetArgGluValAspArg420425430gctcttttagcaatgcctgagtacaacaagggtgacatgatggttgtt1344AlaLeuLeuAlaMetProGluTyrAsnLysGlyAspMetMetValVal435440445gttgcaggttcccctcctggtgttaccggtaacaccaacatgattcac1392ValAlaGlySerProProGlyValThrGlyAsnThrAsnMetIleHis450455460gtccaccttcttggtgacgacacaaggattgcaaagctc1431ValHisLeuLeuGlyAspAspThrArgIleAlaLysLeu465470475<210>22<211>477<212>PRT<213>谷氨酸棒杆菌<400>22MetGlyValAspArgArgThrLysIleValCysThrLeuGlyProAla151015ValAlaSerAlaAspGlyIleLeuArgLeuValGluAspGlyMetAsp202530ValAlaArgLeuAsnPheSerHisGlyAspHisProAspHisGluGln354045AsnTyrLysTrpValArgGluAlaAlaGluLysThrGlyArgAlaVal505560GlyIleLeuAlaAspLeuGlnGlyProLysIleArgLeuGlyArgPhe65707580ThrAspGlyAlaThrValTrpGluAsnGlyGluThrIleArgIleThr859095ValAspAspValGluGlyThrHisAspArgValSerThrThrTyrLys100105110AsnLeuAlaLysAspAlaLysProGlyAspArgLeuLeuValAspAsp115120125GlyLysValGlyLeuValCysValSerValGluGlyAsnAspValIle130135140CysGluValValGluGlyGlyProValSerAsnAsnLysGlyValSer145150155160LeuProGlyMetAspIleSerValProAlaLeuSerGluLysAspIle165170175ArgAspLeuArgPheAlaLeuLysLeuGlyValAspPheIleAlaLeu180185190SerPheValArgSerProAlaAspAlaGluLeuValHisLysIleMet195200205AspGluGluGlyArgArgValProValIleAlaLysLeuGluLysPro210215220GluAlaValThrSerLeuGluProIleValLeuAlaPheAspAlaVal225230235240MetValAlaArgGlyAspLeuGlyValGluValProLeuGluGluVal245250255ProLeuValGlnLysArgAlaIleGlnIleAlaArgGluAsnAlaLys260265270ProValIleValAlaThrGlnMetLeuAspSerMetIleGluAsnSer275280285ArgProThrArgAlaGluAlaSerAspValAlaAsnAlaValLeuAsp290295300GlyAlaAspAlaValMetLeuSerGlyGluThrSerValGlyLysAsp305310315320ProHisAsnValValArgThrMetSerArgIleValArgPheAlaGlu325330335ThrAspGlyArgValProAspLeuThrHisIleProArgThrLysArg340345350GlyValIleSerTyrSerAlaArgAspIleAlaGluArgLeuAsnAla355360365ArgAlaLeuValAlaPheThrThrSerGlyAspThrAlaLysArgVal370375380AlaArgLeuHisSerHisLeuProLeuLeuValPheThrProAsnGlu385390395400AlaValArgSerGluLeuAlaLeuThrTrpGlyAlaThrThrPheLeu405410415CysProProValSerAspThrAspAspMetMetArgGluValAspArg420425430AlaLeuLeuAlaMetProGluTyrAsnLysGlyAspMetMetValVal435440445ValAlaGlySerProProGlyValThrGlyAsnThrAsnMetIleHis450455460ValHisLeuLeuGlyAspAspThrArgIleAlaLysLeu465470475<210>23<211>1704<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1704)<223>RXA01244W<400>23gtggctactgtggctgatgtgaatcaagacactgtactgaagggcacc48MetAlaThrValAlaAspValAsnGlnAspThrValLeuLysGlyThr151015ggcgttgtcggtggagtccgttatgcaagcgcggtgtggattacccca96GlyValValGlyGlyValArgTyrAlaSerAlaValTrpIleThrPro202530cgccccgaactaccccaagcaggcgaagtcgtcgccgaagaaaaccgt144ArgProGluLeuProGlnAlaGlyGluValValAlaGluGluAsnArg354045gaagcagagcaggagcgtttcgacgccgctgcagccacagtctcttct192GluAlaGluGlnGluArgPheAspAlaAlaAlaAlaThrValSerSer505560cgtttgcttgagcgctccgaagctgctgaaggaccagcagctgaggtg240ArgLeuLeuGluArgSerGluAlaAlaGluGlyProAlaAlaGluVal65707580cttaaagctactgctggcatggtcaatgaccgtggctggcgtaaggct288LeuLysAlaThrAlaGlyMetValAsnAspArgGlyTrpArgLysAla859095gtcatcaagggtgtcaagggtggtcaccctgcggaatacgccgtggtt336ValIleLysGlyValLysGlyGlyHisProAlaGluTyrAlaValVal100105110gcagcaacaaccaagttcatctccatgttcgaagccgcaggcggcctg384AlaAlaThrThrLysPheIleSerMetPheGluAlaAlaGlyGlyLeu115120125atcgcggagcgcaccacagacttgcgcgacatccgcgaccgcgtcatc432IleAlaGluArgThrThrAspLeuArgAspIleArgAspArgValIle130135140gcagaacttcgtggcgatgaagagccaggtctgccagctgtttccgga480AlaGluLeuArgGlyAspGluGluProGlyLeuProAlaValSerGly145150155160caggtcattctctttgcagatgacctctccccagcagacaccgcggca528GlnValIleLeuPheAlaAspAspLeuSerProAlaAspThrAlaAla165170175ctagacacagatctctttgtgggacttgtcactgagctgggtggccca576LeuAspThrAspLeuPheValGlyLeuValThrGluLeuGlyGlyPro180185190acgagccacaccgcgatcatcgcacgccagctcaacgtgccttgcatc624ThrSerHisThrAlaIleIleAlaArgGlnLeuAsnValProCysIle195200205gtcgcatccggcgccggcatcaaggacatcaagtccggcgaaaaggtg672ValAlaSerGlyAlaGlyIleLysAspIleLysSerGlyGluLysVal210215220cttatcgacggcagcctcggcaccattgaccgcaacgcggacgaagct720LeuIleAspGlySerLeuGlyThrIleAspArgAsnAlaAspGluAla225230235240gaagcaaccaagctcgtctccgagtccctcgagcgcgctgctcgcatc768GluAlaThrLysLeuValSerGluSerLeuGluArgAlaAlaArgIle245250255gccgagtggaagggtcctgcacaaaccaaggacggctaccgcgttcag816AlaGluTrpLysGlyProAlaGlnThrLysAspGlyTyrArgValGln260265270ctgttggccaacgtccaagacggcaactctgcacagcaggctgcacag864LeuLeuAlaAsnValGlnAspGlyAsnSerAlaGlnGlnAlaAlaGln275280285accgaagcagaaggcatcggcctgttccgcaccgaactgtgcttcctt912ThrGluAlaGluGlyIleGlyLeuPheArgThrGluLeuCysPheLeu290295300tccgccaccgaagagccaagcgttgatgagcaggctgcggtctactca960SerAlaThrGluGluProSerValAspGluGlnAlaAlaValTyrSer305310315320aaggtgcttgaagcattcccagagtccaaggtcgttgtccgctccctc1008LysValLeuGluAlaPheProGluSerLysValValValArgSerLeu325330335gacgcaggttctgacaagccagttccattcgcatcgatggctgatgag1056AspAlaGlySerAspLysProValProPheAlaSerMetAlaAspGlu340345350atgaacccagcactgggtgttcgtggcctgcgtatcgcacgtggacag1104MetAsnProAlaLeuGlyValArgGlyLeuArgIleAlaArgGlyGln355360365gttgatctgctgactcgccagctcgacgcaattgcgaaggccagcgaa1152ValAspLeuLeuThrArgGlnLeuAspAlaIleAlaLysAlaSerGlu370375380gaactcggccgtggcgacgacgccccaacctgggttatggctccaatg1200GluLeuGlyArgGlyAspAspAlaProThrTrpValMetAlaProMet385390395400gtggctaccgcttatgaagcaaagtggtttgctgacatgtgccgtgag1248ValAlaThrAlaTyrGluAlaLysTrpPheAlaAspMetCysArgGlu405410415cgtggcctaatcgccggcgccatgatcgaagttccagcagcatccctg1296ArgGlyLeuIleAlaGlyAlaMetIleGluValProAlaAlaSerLeu420425430atggcagacaagatcatgcctcacctggactttgtttccatcggtacc1344MetAlaAspLysIleMetProHisLeuAspPheValSerIleGlyThr435440445aacgacctgacccagtacaccatggcagcggaccgcatgtctcctgag1392AsnAspLeuThrGlnTyrThrMetAlaAlaAspArgMetSerProGlu450455460cttgcctacctgaccgatccttggcagccagcagtcctgcgcctgatc1440LeuAlaTyrLeuThrAspProTrpGlnProAlaValLeuArgLeuIle465470475480aagcacacctgtgacgaaggtgctcgctttaacaccccggtcggtgtt1488LysHisThrCysAspGluGlyAlaArgPheAsnThrProValGlyVal485490495tgtggtgaagcagcagcagacccactgttggcaactgtcctcaccggt1536CysGlyGluAlaAlaAlaAspProLeuLeuAlaThrValLeuThrGly500505510cttggcgtgaactccctgtccgcagcatccactgctctcgcagcagtc1584LeuGlyValAsnSerLeuSerAlaAlaSerThrAlaLeuAlaAlaVal515520525ggtgcaaagctgtcagaggtcaccctggaaacctgtaagaaggcagca1632GlyAlaLysLeuSerGluValThrLeuGluThrCysLysLysAlaAla530535540gaagcagcacttgacgctgaaggtgcaactgaagcacgcgatgctgta1680GluAlaAlaLeuAspAlaGluGlyAlaThrGluAlaArgAspAlaVal545550555560cgcgcagtgatcgacgcagcagtc1704ArgAlaValIleAspAlaAlaVal565<210>24<211>568<212>PRT<213>谷氨酸棒杆菌<400>24MetAlaThrValAlaAspValAsnGlnAspThrValLeuLysGlyThr151015GlyValValGlyGlyValArgTyrAlaSerAlaValTrpIleThrPro202530ArgProGluLeuProGlnAlaGlyGluValValAlaGluGluAsnArg354045GluAlaGluGlnGluArgPheAspAlaAlaAlaAlaThrValSerSer505560ArgLeuLeuGluArgSerGluAlaAlaGluGlyProAlaAlaGluVal65707580LeuLysAlaThrAlaGlyMetValAsnAspArgGlyTrpArgLysAla859095ValIleLysGlyValLysGlyGlyHisProAlaGluTyrAlaValVal100105110AlaAlaThrThrLysPheIleSerMetPheGluAlaAlaGlyGlyLeu115120125IleAlaGluArgThrThrAspLeuArgAspIleArgAspArgValIle130135140AlaGluLeuArgGlyAspGluGluProGlyLeuProAlaValSerGly145150155160GlnValIleLeuPheAlaAspAspLeuSerProAlaAspThrAlaAla165170175LeuAspThrAspLeuPheValGlyLeuValThrGluLeuGlyGlyPro180185190ThrSerHisThrAlaIleIleAlaArgGlnLeuAsnValProCysIle195200205ValAlaSerGlyAlaGlyIleLysAspIleLysSerGlyGluLysVal210215220LeuIleAspGlySerLeuGlyThrIleAspArgAsnAlaAspGluAla225230235240GluAlaThrLysLeuValSerGluSerLeuGluArgAlaAlaArgIle245250255AlaGluTrpLysGlyProAlaGlnThrLysAspGlyTyrArgValGln260265270LeuLeuAlaAsnValGlnAspGlyAsnSerAlaGlnGlnAlaAlaGln275280285ThrGluAlaGluGlyIleGlyLeuPheArgThrGluLeuCysPheLeu290295300SerAlaThrGluGluProSerValAspGluGlnAlaAlaValTyrSer305310315320LysValLeuGluAlaPheProGluSerLysValValValArgSerLeu325330335AspAlaGlySerAspLysProValProPheAlaSerMetAlaAspGlu340345350MetAsnProAlaLeuGlyValArgGlyLeuArgIleAlaArgGlyGln355360365ValAspLeuLeuThrArgGlnLeuAspAlaIleAlaLysAlaSerGlu370375380GluLeuGlyArgGlyAspAspAlaProThrTrpValMetAlaProMet385390395400ValAlaThrAlaTyrGluAlaLysTrpPheAlaAspMetCysArgGlu405410415ArgGlyLeuIleAlaGlyAlaMetIleGluValProAlaAlaSerLeu420425430MetAlaAspLysIleMetProHisLeuAspPheValSerIleGlyThr435440445AsnAspLeuThrGlnTyrThrMetAlaAlaAspArgMetSerProGlu450455460LeuAlaTyrLeuThrAspProTrpGlnProAlaValLeuArgLeuIle465470475480LysHisThrCysAspGluGlyAlaArgPheAsnThrProValGlyVal485490495CysGlyGluAlaAlaAlaAspProLeuLeuAlaThrValLeuThrGly500505510LeuGlyValAsnSerLeuSerAlaAlaSerThrAlaLeuAlaAlaVal515520525GlyAlaLysLeuSerGluValThrLeuGluThrCysLysLysAlaAla530535540GluAlaAlaLeuAspAlaGluGlyAlaThrGluAlaArgAspAlaVal545550555560ArgAlaValIleAspAlaAlaVal565<210>25<211>3327<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3249)<223>RXA01434E<400>25gtgagttcttcgctttcgaataattcggagcagtccggcctgcgtggc48MetSerSerSerLeuSerAsnAsnSerGluGlnSerGlyLeuArgGly151015aggatcgttgctccagcaccgccggcgcctgtgcccgaggcgcgcaag96ArgIleValAlaProAlaProProAlaProValProGluAlaArgLys202530aaggctgtcgcacgcacggatggtgatcgctcgagtttgaaaaactcg144LysAlaValAlaArgThrAspGlyAspArgSerSerLeuLysAsnSer354045cctacggcatccgccacccaggcagcccagacgcgcccggcagaaccg192ProThrAlaSerAlaThrGlnAlaAlaGlnThrArgProAlaGluPro505560gaaccaaaaaagcacacctccgattctgatgtggtgcgctcgactggc240GluProLysLysHisThrSerAspSerAspValValArgSerThrGly65707580tccatggcaatagccacgctgctgagtcgtatcaccggtttcctgcgc288SerMetAlaIleAlaThrLeuLeuSerArgIleThrGlyPheLeuArg859095accgtgatgattggtgcggcgctgtcgccggccatcgcttcggcgttc336ThrValMetIleGlyAlaAlaLeuSerProAlaIleAlaSerAlaPhe100105110aacactgccaacacgctgcccaacctgatcactgaaatcgtgttgggt384AsnThrAlaAsnThrLeuProAsnLeuIleThrGluIleValLeuGly115120125gcggtgctgacatcgctggttattccggtccttacccgcgcggaaaaa432AlaValLeuThrSerLeuValIleProValLeuThrArgAlaGluLys130135140gaagacgccgacggcggttccgggttcttcaggcggctgctcaccctg480GluAspAlaAspGlyGlySerGlyPhePheArgArgLeuLeuThrLeu145150155160tcggtgacgctgctgggtggtgtcaccatcctgtcgattatcggcgcg528SerValThrLeuLeuGlyGlyValThrIleLeuSerIleIleGlyAla165170175ccgctgctgacacggatgatgctgtcctctgagggacaagtcaacgtg576ProLeuLeuThrArgMetMetLeuSerSerGluGlyGlnValAsnVal180185190gtcatgtccacggcctttgcgtattggctgctgccacagattttcttc624ValMetSerThrAlaPheAlaTyrTrpLeuLeuProGlnIlePhePhe195200205tacggcctgtttgccctgttcatggctgtgttgaacacccgtgaagtg672TyrGlyLeuPheAlaLeuPheMetAlaValLeuAsnThrArgGluVal210215220ttcaaacccggcgcgtgggcacctgttgtcaacaatgtgatcaccttg720PheLysProGlyAlaTrpAlaProValValAsnAsnValIleThrLeu225230235240accgtgctgggcgtgtacatggtgctgcctgcgcgtttgcacccgcat768ThrValLeuGlyValTyrMetValLeuProAlaArgLeuHisProHis245250255gagcaggtgggcatttttgatccgcagatcattttcctcggcgtgggc816GluGlnValGlyIlePheAspProGlnIleIlePheLeuGlyValGly260265270accacccttggtgtggttgcacagtgtctaatcatgattccgtacctg864ThrThrLeuGlyValValAlaGlnCysLeuIleMetIleProTyrLeu275280285cgtcgcgcgggcattgatatgcgccctctgtggggtatcgatgcgcgt912ArgArgAlaGlyIleAspMetArgProLeuTrpGlyIleAspAlaArg290295300ttgaagcaattcggtggcatggcgatggcgatcatcgtgtacgtggca960LeuLysGlnPheGlyGlyMetAlaMetAlaIleIleValTyrValAla305310315320atctcccagttcggttacatcatcaccactcgcattgcgtcgattgca1008IleSerGlnPheGlyTyrIleIleThrThrArgIleAlaSerIleAla325330335gacgatgctgcgccgtttatttatcagcagcactggatgttgctgcaa1056AspAspAlaAlaProPheIleTyrGlnGlnHisTrpMetLeuLeuGln340345350gttccttatggcatcatcggcgtcaccttgctcaccgcgattatgccg1104ValProTyrGlyIleIleGIyValThrLeuLeuThrAlaIleMetPro355360365cgactgtcccgcaacgcggcagacggcgatgatagggcagtagtctct1152ArgLeuSerArgAsnAlaAlaAspGlyAspAspArgAlaValValSer370375380gaccttcagttgggttccaagctaaccttcatcgcactgatccccatc1200AspLeuGlnLeuGlySerLysLeuThrPheIleAlaLeuIleProIle385390395400gtggtgttcttcaccgccttcggtgtccctattgccaatggccttttt1248ValValPhePheThrAlaPheGlyValProIleAlaAsnGlyLeuPhe405410415gcctacggccaattcgatgccaacgccgccaacatccttggttggact1296AlaTyrGlyGlnPheAspAlaAsnAlaAlaAsnIleLeuGlyTrpThr420425430ctgagcttctctgctttcacgctgattccttacgctttggtgctgcta1344LeuSerPheSerAlaPheThrLeuIleProTyrAlaLeuValLeuLeu435440445catctgcgtgtgttttatgcgcgtgaagaggtctggaccccaaccttc1392HisLeuArgValPheTyrAlaArgGluGluValTrpThrProThrPhe450455460atcatcgccggcatcaccgccaccaaggtcgtgctttccctgttggca1440IleIleAlaGlyIleThrAlaThrLysValValLeuSerLeuLeuAla465470475480ccgctgctgtcgagctccccggagcgtgtggtggtgcttcttggtgcg1488ProLeuLeuSerSerSerProGluArgValValValLeuLeuGlyAla485490495gccaacggtttcagtttcatcaccggcgcggtcatcggcgcgtatctg1536AlaAsnGlyPheSerPheIleThrGlyAlaValIleGlyAlaTyrLeu500505510ttgcgcaacaaactcggcctgttgggtatgcgctctttggctaaaacc1584LeuArgAsnLysLeuGlyLeuLeuGlyMetArgSerLeuAlaLysThr515520525tccctgtgggcgttgggctctgcggcggttggtgcagcagcagcatgg1632SerLeuTrpAlaLeuGlySerAlaAlaValGlyAlaAlaAlaAlaTrp530535540gcgttggggtggctgattcaagccgtcgtgggcgatttcttgctgggc1680AlaLeuGlyTrpLeuIleGlnAlaValValGlyAspPheLeuLeuGly545550555560actctaagctccgtaggctacttgttgaacctggctgtgttgggtgtc1728ThrLeuSerSerValGlyTyrLeuLeuAsnLeuAlaValLeuGlyVal565570575ttcttcatcttcgtcaccggcatcgtgttgtcacgttctggtttgccg1776PhePheIlePheValThrGlyIleValLeuSerArgSerGlyLeuPro580585590gaggtccaaaacttgggccaggcactgacccgcatcccaggtttgagc1824GluValGlnAsnLeuGlyGlnAlaLeuThrArgIleProGlyLeuSer595600605cggtttattcgcccgaataccaagatctctttggatgtcggcgaagtc1872ArgPheIleArgProAsnThrLysIleSerLeuAspValGlyGluVal610615620tccgagcaggatttctccacccagctggtcgcgccaagcgagttcgca1920SerGluGlnAspPheSerThrGlnLeuValAlaProSerGluPheAla625630635640gcaacccctgtgccgccaccgatgtccgccggtattgtccgcggacct1968AlaThrProValProProProMetSerAlaGlyIleValArgGlyPro645650655cgcctggttcccggcgccccagtcggcgacggtcgtttccgtctgctc2016ArgLeuValProGlyAlaProValGlyAspGlyArgPheArgLeuLeu660665670gccgatcacggcggcgtccaaggcgcgcgtttctggcaggcccgcgag2064AlaAspHisGlyGlyValGlnGlyAlaArgPheTrpGlnAlaArgGlu675680685atcgccaccggcaaggaagtcgcgctgatctttgtggatacttccggc2112IleAlaThrGlyLysGluValAlaLeuIlePheValAspThrSerGly690695700aacgccccatttgcgccactgtcttcggcagccgcagcgggcatcgcc2160AsnAlaProPheAlaProLeuSerSerAlaAlaAlaAlaGlyIleAla705710715720tacgaggtgcagcgccgcaccaagaagctggccagcttgggcagcttg2208TyrGluValGlnArgArgThrLysLysLeuAlaSerLeuGlySerLeu725730735gcggtagcccccaacatccactccgaggcgtaccgcaacggttgcctc2256AlaValAlaProAsnlleHisSerGluAlaTyrArgAsnGlyCysLeu740745750attgtggccgattgggtgcctggctccagcttgagcgccgtcgcggaa2304IleValAlaAspTrpValProGlySerSerLeuSerAlaValAlaGlu755760765tccggtgccgatccccgcgccgccgcgttcgcgctcgcggaactaact2352SerGlyAlaAspProArgAlaAlaAlaPheAlaLeuAlaGluLeuThr770775780gaaaccatcggcgaggcccacgagatgggtatcccggccggcttggac2400GluThrIleGlyGluAlaHisGluMetGlyIleProAlaGlyLeuAsp785790795800aacaagtgccgcatccgcatcaacaccgacggccatgccgtcctcgcc2448AsnLysCysArgIleArgIleAsnThrAspGlyHisAlaValLeuAla805810815ttcccggcgattttgcccgatgcctcagagctccgcgacgccaagtcc2496PheProAlaIleLeuProAspAlaSerGluLeuArgAspAlaLysSer820825830ctggcctcggccgccgagatgcttatcgacgcgaccctcgctcccagc2544LeuAlaSerAlaAlaGluMetLeuIleAspAlaThrLeuAlaProSer835840845gacgtcaaggcaatggtcactgaagcccaggggctagctacagaagac2592AspValLysAlaMetValThrGluAlaGlnGlyLeuAlaThrGluAsp850855860aatcccgattacgcatcacttgccatggcgatgcgcacctgcggactg2640AsnProAspTyrAlaSerLeuAlaMetAlaMetArgThrCysGlyLeu865870875880ttcaccgaggaaccaacccaccttgtggtgaagaaggaaaagacacca2688PheThrGluGluProThrHisLeuValValLysLysGluLysThrPro885890895aagcctgcgacacgtgatggtttcggtgcctccgactacaccgtcaag2736LysProAlaThrArgAspGlyPheGlyAlaSerAspTyrThrValLys900905910ggcatggcagccatcgccgctgtggtgatcatcttggtttccctggtg2784GlyMetAlaAlaIleAlaAlaValValIleIleLeuValSerLeuVal915920925gccgccggtaccgcgttcctcaccagcttcttcggcagcagcaccaac2832AlaAlaGlyThrAlaPheLeuThrSerPhePheGlySerSerThrAsn930935940gaacaatccccgttggcctctgttgaagccaccacctctgcaacacca2880GluGlnSerProLeuAlaSerValGluAlaThrThrSerAlaThrPro945950955960gaacctgtggggccaccggtctacctggatctggatcaagcccgcacg2928GluProValGlyProProValTyrLeuAspLeuAspGlnAlaArgThr965970975tgggatgacggtgcaggaacagatgtcaccgacgtcaccgacggcaac2976TrpAspAspGlyAlaGlyThrAspValThrAspValThrAspGlyAsn980985990acctccaccgcatggacctccaccggcggcgacggcctcctagttgac3024ThrSerThrAlaTrpThrSerThrGlyGlyAspGlyLeuLeuValAsp99510001005ctgtccacgcctgcccgcctcgaccgcgtcatcttgaccaccggcacc3072LeuSerThrProAlaArgLeuAspArgValIleLeuThrThrGlyThr101010151020ggctccgacagcaacgtgacctcgaccgtgaagatctacgcattcaac3120GlySerAspSerAsnValThrSerThrValLysIleTyrAlaPheAsn1025103010351040gacgcctcaccacactccctgtcggaaggcatcgagatcggcaccgtg3168AspAlaSerProHisSerLeuSerGluGlyIleGluIleGlyThrVal104510501055gattattccggccgcagtctcagccacagcatccgcgattcctccaag3216AspTyrSerGlyArgSerLeuSerHisSerIleArgAspSerSerLys106010651070cttccgggtcaggtggaatccgtggtgattctggtcgatgaggttcgttcctc3269LeuProGlyGlnValGluSerValValIleLeu10751080acaaacctcagacaccaatccacagatgcagatcgctgaagtacaacttgttggttgg3327<210>26<211>1083<212>PRT<213>谷氨酸棒杆菌<400>26MetSerSerSerLeuSerAsnAsnSerGluGlnSerGlyLeuArgGly151015ArgIleValAlaProAlaProProAlaProValProGluAlaArgLys202530LysAlaValAlaArgThrAspGlyAspArgSerSerLeuLysAsnSer354045ProThrAlaSerAlaThrGlnAlaAlaGlnThrArgProAlaGluPro505560GluProLysLysHisThrSerAspSerAspValValArgSerThrGly65707580SerMetAlaIleAlaThrLeuLeuSerArgIleThrGlyPheLeuArg859095ThrValMetIleGlyAlaAlaLeuSerProAlaIleAlaSerAlaPhe100105110AsnThrAlaAsnThrLeuProAsnLeuIleThrGluIleValLeuGly115120125AlaValLeuThrSerLeuValIleProValLeuThrArgAlaGluLys130135140GluAspAlaAspGlyGlySerGlyPhePheArgArgLeuLeuThrLeu145150155160SerValThrLeuLeuGlyGlyValThrIleLeuSerIleIleGlyAla165170175ProLeuLeuThrArgMetMetLeuSerSerGluGlyGlnValAsnVal180185190ValMetSerThrAlaPheAlaTyrTrpLeuLeuProGlnIlePhePhe195200205TyrGlyLeuPheAlaLeuPheMetAlaValLeuAsnThrArgGluVal210215220PheLysProGlyAlaTrpAlaProValValAsnAsnValIleThrLeu225230235240ThrValLeuGlyValTyrMetValLeuProAlaArgLeuHisProHis245250255GluGlnValGlyIlePheAspProGlnIleIlePheLeuGlyValGly260265270ThrThrLeuGlyValValAlaGlnCysLeuIleMetIleProTyrLeu275280285ArgArgAlaGlyIleAspMetArgProLeuTrpGlyIleAspAlaArg290295300LeuLysGlnPheGlyGlyMetAlaMetAlaIleIleValTyrValAla305310315320IleSerGlnPheGlyTyrIleIleThrThrArgIleAlaSerIleAla325330335AspAspAlaAlaProPheIleTyrGlnGlnHisTrpMetLeuLeuGln340345350ValProTyrGlyIleIleGlyValThrLeuLeuThrAlaIleMetPro355360365ArgLeuSerArgAsnAlaAlaAspGlyAspAspArgAlaValValSer370375380AspLeuGlnLeuGlySerLysLeuThrPheIleAlaLeuIleProIle385390395400ValValPhePheThrAlaPheGlyValProIleAlaAsnGlyLeuPhe405410415AlaTyrGlyGlnPheAspAlaAsnAlaAlaAsnIleLeuGlyTrpThr420425430LeuSerPheSerAlaPheThrLeuIleProTyrAlaLeuValLeuLeu435440445HisLeuArgValPheTyrAlaArgGluGluValTrpThrProThrPhe450455460IleIleAlaGlyIleThrAlaThrLysValValLeuSerLeuLeuAla465470475480ProLeuLeuSerSerSerProGluArgValValValLeuLeuGlyAla485490495AlaAsnGlyPheSerPheIleThrGlyAlaValIleGlyAlaTyrLeu500505510LeuArgAsnLysLeuGlyLeuLeuGlyMetArgSerLeuAlaLysThr515520525SerLeuTrpAlaLeuGlySerAlaAlaValGlyAlaAlaAlaAlaTrp530535540AlaLeuGlyTrpLeuIleGlnAlaValValGlyAspPheLeuLeuGly545550555560ThrLeuSerSerValGlyTyrLeuLeuAsnLeuAlaValLeuGlyVal565570575PhePheIlePheValThrGlyIleValLeuSerArgSerGlyLeuPro580585590GluValGlnAsnLeuGlyGlnAlaLeuThrArgIleProGlyLeuSer595600605ArgPheIleArgProAsnThrLysIleSerLeuAspValGlyGluVal610615620SerGluGlnAspPheSerThrGlnLeuValAlaProSerGluPheAla625630635640AlaThrProValProProProMetSerAlaGlyIleValArgGlyPro645650655ArgLeuValProGlyAlaProValGlyAspGlyArgPheArgLeuLeu660665670AlaAspHisGlyGlyValGlnGlyAlaArgPheTrpGlnAlaArgGlu675680685IleAlaThrGlyLysGluValAlaLeuIlePheValAspThrSerGly690695700AsnAlaProPheAlaProLeuSerSerAlaAlaAlaAlaGlyIleAla705710715720TyrGluValGlnArgArgThrLysLysLeuAlaSerLeuGlySerLeu725730735AlaValAlaProAsnIleHisSerGluAlaTyrArgAsnGlyCysLeu740745750IleValAlaAspTrpValProGlySerSerLeuSerAlaValAlaGlu755760765SerGlyAlaAspProArgAlaAlaAlaPheAlaLeuAlaGluLeuThr770775780GluThrIleGlyGluAlaHisGluMetGlyIleProAlaGlyLeuAsp785790795800AsnLysCysArgIleArgIleAsnThrAspGlyHisAlaValLeuAla805810815PheProAlaIleLeuProAspAlaSerGluLeuArgAspAlaLysSer820825830LeuAlaSerAlaAlaGluMetLeuIleAspAlaThrLeuAlaProSer835840845AspValLysAlaMetValThrGluAlaGlnGlyLeuAlaThrGluAsp850855860AsnProAspTyrAlaSerLeuAlaMetAlaMetArgThrCysGlyLeu865870875880PheThrGluGluProThrHisLeuValValLysLysGluLysThrPro885890895LysProAlaThrArgAspGlyPheGlyAlaSerAspTyrThrValLys900905910GlyMetAlaAlaIleAlaAlaValValIleIleLeuValSerLeuVal915920925AlaAlaGlyThrAlaPheLeuThrSerPhePheGlySerSerThrAsn930935940GluGlnSerProLeuAlaSerValGluAlaThrThrSerAlaThrPro945950955960GluProValGlyProProValTyrLeuAspLeuAspGlnAlaArgThr965970975TrpAspAspGlyAlaGlyThrAspValThrAspValThrAspGlyAsn980985990ThrSerThrAlaTrpThrSerThrGlyGlyAspGlyLeuLeuValAsp99510001005LeuSerThrProAlaArgLeuAspArgValIleLeuThrThrGlyThr101010151020GlySerAspSerAsnValThrSerThrValLysIleTyrAlaPheAsn1025103010351040AspAlaSerProHisSerLeuSerGluGlyIleGluIleGlyThrVal104510501055AspTyrSerGlyArgSerLeuSerHisSerIleArgAspSerSerLys106010651070LeuProGlyGlnValGluSerValValIleLeu10751080<210>27<211>3249<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3249)<223>RXA01434K<400>27gtgcccgaggcgcgcaagaaggctgtcgcacgcacggatggtgatcgc48MetProGluAlaArgLysLysAlaValAlaArgThrAspGlyAspArg151015tcgagtttgaaaaactcgcctacggcatccgccacccaggcagcccag96SerSerLeuLysAsnSerProThrAlaSerAlaThrGlnAlaAlaGln202530acgcgcccggcagaaccggaaccagaaaagcacacctccgattctgat144ThrArgProAlaGluProGluProGluLysHisThrSerAspSerAsp354045gtggtgcgctcgactggctccatggcaatagccacgctgctgagtcgt192ValValArgSerThrGlySerMetAlaIleAlaThrLeuLeuSerArg505560atcaccggtttcctgcgcaccgtgatgattggtgcggcgctgtcgccg240IleThrGlyPheLeuArgThrValMetIleGlyAlaAlaLeuSerPro65707580gccatcgcttcggcgttcaacactgccaacacgctgcccaacctgatc288AlaIleAlaSerAlaPheAsnThrAlaAsnThrLeuProAsnLeuIle859095actgaaatcgtgttgggtgcggtgctgacatcgctggttattccggtc336ThrGluIleValLeuGlyAlaValLeuThrSerLeuValIleProVal100105110cttacccgcgcggaaaaagaagacgccgacggcggttccgggttcttc384LeuThrArgAlaGluLysGluAspAlaAspGlyGlySerGlyPhePhe115120125aggcggctgctcaccctgtcggtgacgctgctgggtggtgtcaccatc432ArgArgLeuLeuThrLeuSerValThrLeuLeuGlyGlyValThrIle130135140ctgtcgattatcggcgcgccgctgctgacacggatgatgctgtcctct480LeuSerIleIleGlyAlaProLeuLeuThrArgMetMetLeuSerSer145150155160gagggacaagtcaacgtggtcatgtccacggcctttgcgtattggctg528GluGlyGlnValAsnValValMetSerThrAlaPheAlaTyrTrpLeu165170175ctgccacagattttcttctacggcctgtttgccctgttcatggctgtg576LeuProGlnIlePhePheTyrGlyLeuPheAlaLeuPheMetAlaVal180185190ttgaacacccgtgaagtgttcaaacccggcgcgtgggcacctgttgtc624LeuAsnThrArgGluValPheLysProGlyAlaTrpAlaProValVal195200205aacaatgtgatcaccttgaccgtgctgggcgtgtacatggtgctgcct672AsnAsnValIleThrLeuThrValLeuGlyValTyrMetValLeuPro210215220gcgcgtttgcacccgcatgagcaggtgggcatttttgatccgcagatc720AlaArgLeuHisProHisGluGlnValGlyIlePheAspProGlnIle225230235240attttcctcggcgtgggcaccacccttggtgtggttgcacagtgtcta768IlePheLeuGlyValGlyThrThrLeuGlyValValAlaGlnCysLeu245250255atcatgattccgtacctgcgtcgcgcgggcattgatatgcgccctctg816IleMetIleProTyrLeuArgArgAlaGlyIleAspMetArgProLeu260265270tggggtatcgatgcgcgtttgaagcaattcggtggcatggcgatggcg864TrpGlyIleAspAlaArgLeuLysGlnPheGlyGlyMetAlaMetAla275280285atcatcgtgtacgtggcaatctcccagttcggttacatcatcaccact912IleIleValTyrValAlaIleSerGlnPheGlyTyrIleIleThrThr290295300cgcattgcgtcgattgcagacgatgctgcgccgtttatttatcagcag960ArgIleAlaSerIleAlaAspAspAlaAlaProPheIleTyrGlnGln305310315320cactggatgttgctgcaagttccttatggcatcatcggcgtcaccttg1008HisTrpMetLeuLeuGlnValProTyrGlyIleIleGlyValThrLeu325330335ctcaccgcgattatgccgcgactgtcccgcaacgcggcagacggcgat1056LeuThrAlaIleMetProArgLeuSerArgAsnAlaAlaAspGlyAsp340345350gatagggcagtagtctctgaccttcagttgggttccaagctaaccttc1104AspArgAlaValValSerAspLeuGlnLeuGlySerLysLeuThrPhe355360365atcgcactgatccccatcgtggtgttcttcaccgccttcggtgtccct1152IleAlaLeuIleProIleValValPhePheThrAlaPheGlyValPro370375380attgccaatggcctttttgcctacggccaattcgatgccaacgccgcc1200IleAlaAsnGlyLeuPheAlaTyrGlyGlnPheAspAlaAsnAlaAla385390395400aacatccttggttggactctgagcttctctgctttcacgctgattcct1248AsnIleLeuGlyTrpThrLeuSerPheSerAlaPheThrLeuIlePro405410415tacgctttggtgctgctacatctgcgtgtgttttatgcgcgtgaagag1296TyrAlaLeuValLeuLeuHisLeuArgValPheTyrAlaArgGluGlu420425430gtctggaccccaaccttcatcatcgccggcatcaccgccaccaaggtc1344ValTrpThrProThrPheIleIleAlaGlyIleThrAlaThrLysVal435440445gtgctttccctgttggcaccgctgctgtcgagctccccggagcgtgtg1392ValLeuSerLeuLeuAlaProLeuLeuSerSerSerProGluArgVal450455460gtggtgcttcttggtgcggccaacggtttcagtttcatcaccggcgcg1440ValValLeuLeuGlyAlaAlaAsnGlyPheSerPheIleThrGlyAla465470475480gtcatcggcgcgtatctgttgcgcaacaaactcggcctgttgggtatg1488ValIleGlyAlaTyrLeuLeuArgAsnLysLeuGlyLeuLeuGlyMet485490495cgctctttggctaaaacctccctgtgggcgttgggctctgcggcggtt1536ArgSerLeuAlaLysThrSerLeuTrpAlaLeuGlySerAlaAlaVal500505510ggtgcagcagcagcatgggcgttggggtggctgattcaagccgtcgtg1584GlyAlaAlaAlaAlaTrpAlaLeuGlyTrpLeuIleGlnAlaValVal515520525ggcgatttcttgctgggcactctaagctccgtaggctacttgttgtac1632GlyAspPheLeuLeuGlyThrLeuSerSerValGlyTyrLeuLeuTyr530535540ctggctgtgttgggtgtcttcttcatcttcgtcaccggcatcgtgttg1680LeuAlaValLeuGlyValPhePheIlePheValThrGlyIleValLeu545550555560tcacgttctggtttgccggaggtccaaaacttgggccaggcactgacc1728SerArgSerGlyLeuProGluValGlnAsnLeuGlyGlnAlaLeuThr565570575cgcatcccaggtttgagccggtttattcgcccgaataccaagatctct1776ArgIleProGlyLeuSerArgPheIleArgProAsnThrLysIleSer580585590ttggatgtcggcgaagtctccgagcaggatttctccacccagctggtc1824LeuAspValGlyGluValSerGluGlnAspPheSerThrGlnLeuVal595600605gcgccaagcgagttcgcagcaacccctgtgccgccaccgatgtccgcc1872AlaProSerGluPheAlaAlaThrProValProProProMetSerAla610615620ggtattgtccgcggacctcgcctggttcccggcgccccagtcggcgac1920GlyIleValArgGlyProArgLeuValProGlyAlaProValGlyAsp625630635640ggtcgtttccgtctgctcgccgatcacggcggcgtccaaggcgcgcgt1968GlyArgPheArgLeuLeuAlaAspHisGlyGlyValGlnGlyAlaArg645650655ttctggcaggcccgcgagatcgccaccggcaaggaagtcgcgctgatc2016PheTrpGlnAlaArgGluIleAlaThrGlyLysGluValAlaLeuIle660665670tttgtggatacttccggcaacgccccatttgcgccactgtcttcggca2064PheValAspThrSerGlyAsnAlaProPheAlaProLeuSerSerAla675680685gccgcagcgggcatcgcctacgaggtgcagcgccgcaccaagaagctg2112AlaAlaAlaGlyIleAlaTyrGluValGlnArgArgThrLysLysLeu690695700gccagcttgggcagcttggcggtagcccccaacatccactccgaggcg2160AlaSerLeuGlySerLeuAlaValAlaProAsnIleHisSerGluAla705710715720taccgcaacggttgcctcattgtggccgattgggtgcctggctccagc2208TyrArgAsnGlyCysLeuIleValAlaAspTrpValProGlySerSer725730735ttgagcgccgtcgcggaatccggtgccgatccccgcgccgccgcgttc2256LeuSerAlaValAlaGluSerGlyAlaAspProArgAlaAlaAlaPhe740745750gcgctcgcggaactaactgaaaccatcggcgaggcccacgagatgggt2304AlaLeuAlaGluLeuThrGluThrIleGlyGluAlaHisGluMetGly755760765atcccggccggcttggacaacaagtgccgcatccgcatcaacaccgac2352IleProAlaGlyLeuAspAsnLysCysArgIleArgIleAsnThrAsp770775780ggccatgccgtcctcgccttcccggcgattttgcccgatgcctcagag2400GlyHisAlaValLeuAlaPheProAlaIleLeuProAspAlaSerGlu785790795800ctccgcgacgccaagtccctggcctcggccgccgagatgcttatcgac2448LeuArgAspAlaLysSerLeuAlaSerAlaAlaGluMetLeuIleAsp805810815gcgaccctcgctcccagcgacgtcaaggcaatggtcactgaagcccag2496AlaThrLeuAlaProSerAspValLysAlaMetValThrGluAlaGln820825830gggctagctacagaagacaatcccgattacgcatcacttgccatggcg2544GlyLeuAlaThrGluAspAsnProAspTyrAlaSerLeuAlaMetAla835840845atgcgcacctgcggactgttcaccgaggaaccaacccaccttgtggtg2592MetArgThrCysGlyLeuPheThrGluGluProThrHisLeuValVal850855860aagaaggaaaagacaccaaagcctgcgacacgtgatggtttcggtgcc2640LysLysGluLysThrProLysProAlaThrArgAspGlyPheGlyAla865870875880tccgactacaccgtcaagggcatggcagccatcgccgctgtggtgatc2688SerAspTyrThrValLysGlyMetAlaAlaIleAlaAlaValValIle885890895atcttggtttccctggtggccgccggtaccgcgttcctcaccagcttc2736IleLeuValSerLeuValAlaAlaGlyThrAlaPheLeuThrSerPhe900905910ttcggcagcagcaccaacgaacaatccccgttggcctctgttgaagcc2784PheGlySerSerThrAsnGluGlnSerProLeuAlaSerValGluAla915920925accacctctgcaacaccagaacctgtggggccaccggtctacctggat2832ThrThrSerAlaThrProGluProValGlyProProValTyrLeuAsp930935940ctggatcaagcccgcacgtgggatgacggtgcaggaacagatgtcacc2880LeuAspGlnAlaArgThrTrpAspAspGlyAlaGlyThrAspValThr945950955960gacgtcaccgacggcaacacctccaccgcatggacctccaccggcggc2928AspValThrAspGlyAsnThrSerThrAlaTrpThrSerThrGlyGly965970975gacggcctcctagttgacctgtccacgcctgcccgcctcgaccgcgtc2976AspGlyLeuLeuValAspLeuSerThrProAlaArgLeuAspArgVal980985990atcttgaccaccggcaccggctccgacagcaacgtgacctcgaccgtg3024IleLeuThrThrGlyThrGlySerAspSerAsnValThrSerThrVal99510001005aagatctacgcattcaacgacgcctcaccacactccctgtcggaaggc3072LysIleTyrAlaPheAsnAspAlaSerProHisSerLeuSerGluGly101010151020atcgagatcggcaccgtggattattccggccgcagtctcagccacagc3120IleGluIleGlyThrValAspTyrSerGlyArgSerLeuSerHisSer1025103010351040atccgcgattcctccaagcttccgggtcaggtggaatccgtggtgatt3168IleArgAspSerSerLysLeuProGlyGlnValGluSerValValIle104510501055ctggtcgatgaggttcgttcctcacaaacctcagacaccaatccacag3216LeuValAspGluValArgSerSerGlnThrSerAspThrAsnProGln106010651070atgcagatcgctgaagtacaacttgttggttgg3249MetGlnIleAlaGluValGlnLeuValGlyTrp10751080<210>28<211>1083<212>PRT<213>谷氨酸棒杆菌<400>28MetProGluAlaArgLysLysAlaValAlaArgThrAspGlyAspArg151015SerSerLeuLysAsnSerProThrAlaSerAlaThrGlnAlaAlaGln202530ThrArgProAlaGluProGluProGluLysHisThrSerAspSerAsp354045ValValArgSerThrGlySerMetAlaIleAlaThrLeuLeuSerArg505560IleThrGlyPheLeuArgThrValMetIleGlyAlaAlaLeuSerPro65707580AlaIleAlaSerAlaPheAsnThrAlaAsnThrLeuProAsnLeuIle859095ThrGluIleValLeuGlyAlaValLeuThrSerLeuValIleProVal100105110LeuThrArgAlaGluLysGluAspAlaAspGlyGlySerGlyPhePhe115120125ArgArgLeuLeuThrLeuSerValThrLeuLeuGlyGlyValThrIle130135140LeuSerIleIleGlyAlaProLeuLeuThrArgMetMetLeuSerSer145150155160GluGlyGlnValAsnValValMetSerThrAlaPheAlaTyrTrpLeu165170175LeuProGlnIlePhePheTyrGlyLeuPheAlaLeuPheMetAlaVal180185190LeuAsnThrArgGluValPheLysProGlyAlaTrpAlaProValVal195200205AsnAsnValIleThrLeuThrValLeuGlyValTyrMetValLeuPro210215220AlaArgLeuHisProHisGluGlnValGlyIlePheAspProGlnIle225230235240IlePheLeuGlyValGlyThrThrLeuGlyValValAlaGlnCysLeu245250255IleMetIleProTyrLeuArgArgAlaGlyIleAspMetArgProLeu260265270TrpGlyIleAspAlaArgLeuLysGlnPheGlyGlyMetAlaMetAla275280285IleIleValTyrValAlaIleSerGlnPheGlyTyrIleIleThrThr290295300ArgIleAlaSerIleAlaAspAspAlaAlaProPheIleTyrGlnGln305310315320HisTrpMetLeuLeuGlnValProTyrGlyIleIleGlyValThrLeu325330335LeuThrAlaIleMetProArgLeuSerArgAsnAlaAlaAspGlyAsp340345350AspArgAlaValValSerAspLeuGlnLeuGlySerLysLeuThrPhe355360365IleAlaLeuIleProIleValValPhePheThrAlaPheGlyValPro370375380IleAlaAsnGlyLeuPheAlaTyrGlyGlnPheAspAlaAsnAlaAla385390395400AsnIleLeuGlyTrpThrLeuSerPheSerAlaPheThrLeuIlePro405410415TyrAlaLeuValLeuLeuHisLeuArgValPheTyrAlaArgGluGlu420425430ValTrpThrProThrPheIleIleAlaGlyIleThrAlaThrLysVal435440445ValLeuSerLeuLeuAlaProLeuLeuSerSerSerProGluArgVal450455460ValValLeuLeuGlyAlaAlaAsnGlyPheSerPheIleThrGlyAla465470475480ValIleGlyAlaTyrLeuLeuArgAsnLysLeuGlyLeuLeuGlyMet485490495ArgSerLeuAlaLysThrSerLeuTrpAlaLeuGlySerAlaAlaVal500505510GlyAlaAlaAlaAlaTrpAlaLeuGlyTrpLeuIleGlnAlaValVal515520525GlyAspPheLeuLeuGlyThrLeuSerSerValGlyTyrLeuLeuTyr530535540LeuAlaValLeuGlyValPhePheIlePheValThrGlyIleValLeu545550555560SerArgSerGlyLeuProGluValGlnAsnLeuGlyGlnAlaLeuThr565570575ArgIleProGlyLeuSerArgPheIleArgProAsnThrLysIleSer580585590LeuAspValGlyGluValSerGluGlnAspPheSerThrGlnLeuVal595600605AlaProSerGluPheAlaAlaThrProValProProProMetSerAla610615620GlyIleValArgGlyProArgLeuValProGlyAlaProValGlyAsp625630635640GlyArgPheArgLeuLeuAlaAspHisGlyGlyValGlnGlyAlaArg645650655PheTrpGlnAlaArgGluIleAlaThrGlyLysGluValAlaLeuIle660665670PheValAspThrSerGlyAsnAlaProPheAlaProLeuSerSerAla675680685AlaAlaAlaGlyIleAlaTyrGluValGlnArgArgThrLysLysLeu690695700AlaSerLeuGlySerLeuAlaValAlaProAsnIleHisSerGluAla705710715720TyrArgAsnGlyCysLeuIleValAlaAspTrpValProGlySerSer725730735LeuSerAlaValAlaGluSerGlyAlaAspProArgAlaAlaAlaPhe740745750AlaLeuAlaGluLeuThrGluThrIleGlyGluAlaHisGluMetGly755760765IleProAlaGlyLeuAspAsnLysCysArgIleArgIleAsnThrAsp770775780GlyHisAlaValLeuAlaPheProAlaIleLeuProAspAlaSerGlu785790795800LeuArgAspAlaLysSerLeuAlaSerAlaAlaGluMetLeuIleAsp805810815AlaThrLeuAlaProSerAspValLysAlaMetValThrGluAlaGln820825830GlyLeuAlaThrGluAspAsnProAspTyrAlaSerLeuAlaMetAla835840845MetArgThrCysGlyLeuPheThrGluGluProThrHisLeuValVal850855860LysLysGluLysThrProLysProAlaThrArgAspGlyPheGlyAla865870875880SerAspTyrThrValLysGlyMetAlaAlaIleAlaAlaValValIle885890895IleLeuValSerLeuValAlaAlaGlyThrAlaPheLeuThrSerPhe900905910PheGlySerSerThrAsnGluGlnSerProLeuAlaSerValGluAla915920925ThrThrSerAlaThrProGluProValGlyProProValTyrLeuAsp930935940LeuAspGlnAlaArgThrTrpAspAspGlyAlaGlyThrAspValThr945950955960AspValThrAspGlyAsnThrSerThrAlaTrpThrSerThrGlyGly965970975AspGlyLeuLeuValAspLeuSerThrProAlaArgLeuAspArgVal980985990IleLeuThrThrGlyThrGlySerAspSerAsnValThrSerThrVal99510001005LysIleTyrAlaPheAsnAspAlaSerProHisSerLeuSerGluGly101010151020IleGluIleGlyThrValAspTyrSerGlyArgSerLeuSerHisSer1025103010351040IleArgAspSerSerLysLeuProGlyGlnValGluSerValValIle104510501055LeuValAspGluValArgSerSerGlnThrSerAspThrAsnProGln106010651070MetGlnIleAlaGluValGlnLeuValGlyTrp10751080<210>29<211>3723<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3723)<223>RXA02056E<400>29gtggataaaacaaagctcaataaaccctcaagaagcaaggaaaagagg48MetAspLysThrLysLeuAsnLysProSerArgSerLysGluLysArg151015cgagtacctgccgtgagcagcgctagtactttcggccagaatgcgtgg96ArgValProAlaValSerSerAlaSerThrPheGlyGlnAsnAlaTrp202530ctggtagacgagatgttccagcagttccagaaggaccccaagtccgtg144LeuValAspGluMetPheGlnGlnPheGlnLysAspProLysSerVal354045gacaaggaatggagagaactctttgaggcgcaggggggaccaaatact192AspLysGluTrpArgGluLeuPheGluAlaGlnGlyGlyProAsnThr505560acccccgctacaacagaagcacagccttcagcgcccaaggagtctgcg240ThrProAlaThrThrGluAlaGlnProSerAlaProLysGluSerAla65707580aaaccagcaccaaaggctgcccctgcagccaaggcagcaccgcgcgta288LysProAlaProLysAlaAlaProAlaAlaLysAlaAlaProArgVal859095gaaaccaagccggccgacaagaccgcccctaaggccaaggagtcctca336GluThrLysProAlaAspLysThrAlaProLysAlaLysGluSerSer100105110gtgccacagcaacctaagcttccggagccaggacaaaccccaatcagg384ValProGlnGlnProLysLeuProGluProGlyGlnThrProIleArg115120125ggtattttcaagtccatcgcgaagaacatggatatctccctggaaatc432GlyIlePheLysSerIleAlaLysAsnMetAspIleSerLeuGluIle130135140ccaaccgcaacctcggttcgcgatatgccagctcgcctcatgttcgaa480ProThrAlaThrSerValArgAspMetProAlaArgLeuMetPheGlu145150155160aaccgcgcgatggtcaacgatcagctcaagcgcacccgcggtggcaag528AsnArgAlaMetValAsnAspGlnLeuLysArgThrArgGlyGlyLys165170175atctccttcacccacatcattggctacgccatggtgaaggcagtcatg576IleSerPheThrHisIleIleGlyTyrAlaMetValLysAlaValMet180185190gctcacccggacatgaacaactcctacgacgtcatcgacggcaagcca624AlaHisProAspMetAsnAsnSerTyrAspValIleAspGlyLysPro195200205accctgatcgtgcctgagcacatcaacctgggccttgctatcgacctt672ThrLeuIleValProGluHisIleAsnLeuGlyLeuAlaIleAspLeu210215220cctcagaaggacggctcccgcgcacttgtcgtagcagccatcaaggaa720ProGlnLysAspGlySerArgAlaLeuValValAlaAlaIleLysGlu225230235240accgagaagatgaacttctccgagttcctcgcagcctacgaagacatc768ThrGluLysMetAsnPheSerGluPheLeuAlaAlaTyrGluAspIle245250255gtggcacgctcccgcaagggcaagctcaccatggatgactaccagggc816ValAlaArgSerArgLysGlyLysLeuThrMetAspAspTyrGlnGly260265270gttaccgtttccttgaccaacccaggtggcatcggtacccgccactct864ValThrValSerLeuThrAsnProGlyGlyIleGlyThrArgHisSer275280285gttccacgtctaaccaagggccagggcaccatcatcggtgtcggttcc912ValProArgLeuThrLysGlyGlnGlyThrIleIleGlyValGlySer290295300atggattacccagcagagttccagggcgcttcagaagaccgccttgca960MetAspTyrProAlaGluPheGlnGlyAlaSerGluAspArgLeuAla305310315320gagctcggcgttggcaaacttgtcaccatcacctccacctacgatcac1008GluLeuGlyValGlyLysLeuValThrIleThrSerThrTyrAspHis325330335cgcgtgatccagggtgctgtgtccggtgaattcctgcgcaccatgtct1056ArgValIleGlnGlyAlaValSerGlyGluPheLeuArgThrMetSer340345350cgcctgctcaccgatgattccttctgggatgagatcttcgacgcaatg1104ArgLeuLeuThrAspAspSerPheTrpAspGluIlePheAspAlaMet355360365aacgttccttacaccccaatgcgttgggcacaggacgttccaaacacc1152AsnValProTyrThrProMetArgTrpAlaGlnAspValProAsnThr370375380ggtgttgataagaacacccgcgtcatgcagctcattgaggcataccgc1200GlyValAspLysAsnThrArgValMetGlnLeuIleGluAlaTyrArg385390395400tcccgtggacacctcatcgctgacaccaacccactttcatgggttcag1248SerArgGlyHisLeuIleAlaAspThrAsnProLeuSerTrpValGln405410415cctggcatgccagttccagaccaccgcgacctcgacatcgagacccac1296ProGlyMetProValProAspHisArgAspLeuAspIleGluThrHis420425430aacctgaccatctgggatctggaccgtaccttcaacgtcggtggcttc1344AsnLeuThrIleTrpAspLeuAspArgThrPheAsnValGlyGlyPhe435440445ggcggcaaggagaccatgaccctgcgcgaggtactgtcccgcctccgc1392GlyGlyLysGluThrMetThrLeuArgGluValLeuSerArgLeuArg450455460gctgcgtacaccctcaaggtcggctccgaatacacccacatcctggac1440AlaAlaTyrThrLeuLysValGlySerGluTyrThrHisIleLeuAsp465470475480cgcgacgagcgcacctggctgcaggaccgcctcgaggccggaatgcca1488ArgAspGluArgThrTrpLeuGlnAspArgLeuGluAlaGlyMetPro485490495aagccaacccaggcagagcagaagtacatcctgcagaagctgaacgcc1536LysProThrGlnAlaGluGlnLysTyrIleLeuGlnLysLeuAsnAla500505510gcggaggctttcgagaacttcctgcagaccaagtacgtcggccagaag1584AlaGluAlaPheGluAsnPheLeuGlnThrLysTyrValGlyGlnLys515520525cgcttctccctcgaaggtgcagaagcacttatcccactgatggactcc1632ArgPheSerLeuGluGlyAlaGluAlaLeuIleProLeuMetAspSer530535540gccatcgacaccgccgcaggccaaggcctcgacgaagttgtcatcggt1680AlaIleAspThrAlaAlaGlyGlnGlyLeuAspGluValValIleGly545550555560atgccacaccgtggtcgcctcaacgtgctgttcaacatcgtgggcaag1728MetProHisArgGlyArgLeuAsnValLeuPheAsnIleValGlyLys565570575ccactggcatccatcttcaacgagtttgaaggccaaatggagcagggc1776ProLeuAlaSerIlePheAsnGluPheGluGlyGlnMetGluGlnGly580585590cagatcggtggctccggtgacgtgaagtaccacctcggttccgaaggc1824GlnIleGlyGlySerGlyAspValLysTyrHisLeuGlySerGluGly595600605cagcacctgcagatgttcggcgacggcgagatcaaggtctccctgact1872GlnHisLeuGlnMetPheGlyAspGlyGluIleLysValSerLeuThr610615620gctaacccgtcccacctggaagctgttaacccagtgatggaaggtatc1920AlaAsnProSerHisLeuGluAlaValAsnProValMetGluGlyIle625630635640gtccgcgcaaagcaggactacctggacaagggcgtagacggcaagact1968ValArgAlaLysGlnAspTyrLeuAspLysGlyValAspGlyLysThr645650655gttgtgccactgctgctccacggtgacgctgcattcgcaggcctgggc2016ValValProLeuLeuLeuHisGlyAspAlaAlaPheAlaGlyLeuGly660665670atcgtgccagaaaccatcaacctggctaagctgcgtggctacgacgtc2064IleValProGluThrIleAsnLeuAlaLysLeuArgGlyTyrAspVal675680685ggcggcaccatccacatcgtggtgaacaaccagatcggcttcaccacc2112GlyGlyThrIleHisIleValValAsnAsnGlnIleGlyPheThrThr690695700accccagactccagccgctccatgcactacgcaaccgactacgccaag2160ThrProAspSerSerArgSerMetHisTyrAlaThrAspTyrAlaLys705710715720gcattcggctgcccagtcttccacgtcaacggcgacgacccagaggca2208AlaPheGlyCysProValPheHisValAsnGlyAspAspProGluAla725730735gttgtctgggttggccagctggccaccgagtaccgtcgtcgcttcggc2256ValValTrpValGlyGlnLeuAlaThrGluTyrArgArgArgPheGly740745750aaggacgtcttcatcgacctcgtctgctaccgcctccgcggccacaac2304LysAspValPheIleAspLeuValCysTyrArgLeuArgGlyHisAsn755760765gaagctgatgatccttccatgacccagccaaagatgtatgagctcatc2352GluAlaAspAspProSerMetThrGlnProLysMetTyrGluLeuIle770775780accggccgcgagaccgttcgtgctcagtacaccgaagacctgctcgga2400ThrGlyArgGluThrValArgAlaGlnTyrThrGluAspLeuLeuGly785790795800cgtggagacctctccaacgaagatgcagaagcagtcgtccgcgacttc2448ArgGlyAspLeuSerAsnGluAspAlaGluAlaValValArgAspPhe805810815cacgaccagatggaatctgtgttcaacgaagtcaaggaaggcggcaag2496HisAspGlnMetGluSerValPheAsnGluValLysGluGlyGlyLys820825830aagcaggctgaggcacagaccggcatcaccggctcccagaagcttcca2544LysGlnAlaGluAlaGlnThrGlyIleThrGlySerGlnLysLeuPro835840845cacggccttgagaccaacatctcccgtgaagagctcctggaactggga2592HisGlyLeuGluThrAsnIleSerArgGluGluLeuLeuGluLeuGly850855860caggctttcgccaacaccccagaaggcttcaactaccacccacgtgtg2640GlnAlaPheAlaAsnThrProGluGlyPheAsnTyrHisProArgVal865870875880gctcccgttgctaagaagcgcgtctcctctgtcaccgaaggtggcatc2688AlaProValAlaLysLysArgValSerSerValThrGluGlyGlyIle885890895gactgggcatggggcgagctcctcgccttcggttccctggctaactcc2736AspTrpAlaTrpGlyGluLeuLeuAlaPheGlySerLeuAlaAsnSer900905910ggccgcttggttcgccttgcaggtgaagattcccgccgcggtaccttc2784GlyArgLeuValArgLeuAlaGlyGluAspSerArgArgGlyThrPhe915920925acccagcgccacgcagttgccatcgacccagcgaccgctgaagagttc2832ThrGlnArgHisAlaValAlaIleAspProAlaThrAlaGluGluPhe930935940aacccactccacgagcttgcacagtccaagggcaacaacggtaagttc2880AsnProLeuHisGluLeuAlaGlnSerLysGlyAsnAsnGlyLysPhe945950955960ctggtctacaactccgcactgaccgagtacgcaggcatgggcttcgag2928LeuValTyrAsnSerAlaLeuThrGluTyrAlaGlyMetGlyPheGlu965970975tacggctactccgtaggaaacgaagactccatcgttgcatgggaagca2976TyrGlyTyrSerValGlyAsnGluAspSerIleValAlaTrpGluAla980985990cagttcggcgacttcgccaacggcgctcagaccatcatcgatgagtac3024GlnPheGlyAspPheAlaAsnGlyAlaGlnThrIleIleAspGluTyr99510001005gtctcctcaggcgaagctaagtggggccagacctccaagctgatcctt3072ValSerSerGlyGluAlaLysTrpGlyGlnThrSerLysLeuIleLeu101010151020ctgctgcctcacggctacgaaggccagggcccagaccactcttccgca3120LeuLeuProHisGlyTyrGluGlyGlnGlyProAspHisSerSerAla1025103010351040cgtatcgagcgcttcctgcagctgtgcgctgagggttccatgactgtt3168ArgIleGluArgPheLeuGlnLeuCysAlaGluGlySerMetThrVal104510501055gctcagccatccaccccagcaaaccacttccacctactgcgtcgtcac3216AlaGlnProSerThrProAlaAsnHisPheHisLeuLeuArgArgHis106010651070gctctgtccgacctgaagcgtccactggttatcttcaccccgaagtcc3264AlaLeuSerAspLeuLysArgProLeuValIlePheThrProLysSer107510801085atgctgcgtaacaaggctgctgcctccgcaccagaagacttcactgag3312MetLeuArgAsnLysAlaAlaAlaSerAlaProGluAspPheThrGlu109010951100gtcaccaagttccagtccgtgatcaacgatccaaacgttgcagatgca3360ValThrLysPheGlnSerValIleAsnAspProAsnValAlaAspAla1105111011151120gccaaggtgaagaaggtcatgctggtctccggcaagctgtactacgaa3408AlaLysValLysLysValMetLeuValSerGlyLysLeuTyrTyrGlu112511301135ttggcaaagcgcaaggagaaggacggacgcgacgacatcgcgatcgtt3456LeuAlaLysArgLysGluLysAspGlyArgAspAspIleAlaIleVal114011451150cgtatcgaaatgctccacccaattccgttcaaccgcatctccgaggct3504ArgIleGluMetLeuHisProIleProPheAsnArgIleSerGluAla115511601165cttgccggctaccctaacgctgaggaagtcctcttcgttcaggatgag3552LeuAlaGlyTyrProAsnAlaGluGluValLeuPheValGlnAspGlu117011751180ccagcaaaccagggcccatggccgttctaccaggagcacctcccagag3600ProAlaAsnGlnGlyProTrpProPheTyrGlnGluHisLeuProGlu1185119011951200ctgatcccgaacatgccaaagatgcgccgcgtttcccgccgcgctcag3648LeuIleProAsnMetProLysMetArgArgValSerArgArgAlaGln120512101215tcctccaccgcaactggtgttgccaaggtgcaccagctggaggagaag3696SerSerThrAlaThrGlyValAlaLysValHisGlnLeuGluGluLys122012251230cagcttatcgacgaggctttcgaggct3723GlnLeuIleAspGluAlaPheGluAla12351240<210>30<211>1241<212>PRT<213>谷氨酸棒杆菌<400>30MetAspLysThrLysLeuAsnLysProSerArgSerLysGluLysArg151015ArgValProAlaValSerSerAlaSerThrPheGlyGlnAsnAlaTrp202530LeuValAspGluMetPheGlnGlnPheGlnLysAspProLysSerVal354045AspLysGluTrpArgGluLeuPheGluAlaGlnGlyGlyProAsnThr505560ThrProAlaThrThrGluAlaGlnProSerAlaProLysGluSerAla65707580LysProAlaProLysAlaAlaProAlaAlaLysAlaAlaProArgVal859095GluThrLysProAlaAspLysThrAlaProLysAlaLysGluSerSer100105110ValProGlnGlnProLysLeuProGluProGlyGlnThrProIleArg115120125GlyIlePheLysSerIleAlaLysAsnMetAspIleSerLeuGluIle130135140ProThrAlaThrSerValArgAspMetProAlaArgLeuMetPheGlu145150155160AsnArgAlaMetValAsnAspGlnLeuLysArgThrArgGlyGlyLys165170175IleSerPheThrHisIleIleGlyTyrAlaMetValLysAlaValMet180185190AlaHisProAspMetAsnAsnSerTyrAspValIleAspGlyLysPro195200205ThrLeuIleValProGluHisIleAsnLeuGlyLeuAlaIleAspLeu210215220ProGlnLysAspGlySerArgAlaLeuValValAlaAlaIleLysGlu225230235240ThrGluLysMetAsnPheSerGluPheLeuAlaAlaTyrGluAspIle245250255ValAlaArgSerArgLysGlyLysLeuThrMetAspAspTyrGlnGly260265270ValThrValSerLeuThrAsnProGlyGlyIleGlyThrArgHisSer275280285ValProArgLeuThrLysGlyGlnGlyThrIleIleGlyValGlySer290295300MetAspTyrProAlaGluPheGlnGlyAlaSerGluAspArgLeuAla305310315320GluLeuGlyValGlyLysLeuValThrIleThrSerThrTyrAspHis325330335ArgValIleGlnGlyAlaValSerGlyGluPheLeuArgThrMetSer340345350ArgLeuLeuThrAspAspSerPheTrpAspGluIlePheAspAlaMet355360365AsnValProTyrThrProMetArgTrpAlaGlnAspValProAsnThr370375380GlyValAspLysAsnThrArgValMetGlnLeuIleGluAlaTyrArg385390395400SerArgGlyHisLeuIleAlaAspThrAsnProLeuSerTrpValGln405410415ProGlyMetProValProAspHisArgAspLeuAspIleGluThrHis420425430AsnLeuThrIleTrpAspLeuAspArgThrPheAsnValGlyGlyPhe435440445GlyGlyLysGluThrMetThrLeuArgGluValLeuSerArgLeuArg450455460AlaAlaTyrThrLeuLysValGlySerGluTyrThrHisIleLeuAsp465470475480ArgAspGluArgThrTrpLeuGlnAspArgLeuGluAlaGlyMetPro485490495LysProThrGlnAlaGluGlnLysTyrIleLeuGlnLysLeuAsnAla500505510AlaGluAlaPheGluAsnPheLeuGlnThrLysTyrValGlyGlnLys515520525ArgPheSerLeuGluGlyAlaGluAlaLeuIleProLeuMetAspSer530535540AlaIleAspThrAlaAlaGlyGlnGlyLeuAspGluValValIleGly545550555560MetProHisArgGlyArgLeuAsnValLeuPheAsnIleValGlyLys565570575ProLeuAlaSerIlePheAsnGluPheGluGlyGlnMetGluGlnGly580585590GlnIleGlyGlySerGlyAspValLysTyrHisLeuGlySerGluGly595600605GlnHisLeuGlnMetPheGlyAspGlyGluIleLysValSerLeuThr610615620AlaAsnProSerHisLeuGluAlaValAsnProValMetGluGlyIle625630635640ValArgAlaLysGlnAspTyrLeuAspLysGlyValAspGlyLysThr645650655ValValProLeuLeuLeuHisGlyAspAlaAlaPheAlaGlyLeuGly660665670IleValProGluThrIleAsnLeuAlaLysLeuArgGlyTyrAspVal675680685GlyGlyThrIleHisIleValValAsnAsnGlnIleGlyPheThrThr690695700ThrProAspSerSerArgSerMetHisTyrAlaThrAspTyrAlaLys705710715720AlaPheGlyCysProValPheHisValAsnGlyAspAspProGluAla725730735ValValTrpValGlyGlnLeuAlaThrGluTyrArgArgArgPheGly740745750LysAspValPheIleAspLeuValCysTyrArgLeuArgGlyHisAsn755760765GluAlaAspAspProSerMetThrGlnProLysMetTyrGluLeuIle770775780ThrGlyArgGluThrValArgAlaGlnTyrThrGluAspLeuLeuGly785790795800ArgGlyAspLeuSerAsnGluAspAlaGluAlaValValArgAspPhe805810815HisAspGlnMetGluSerValPheAsnGluValLysGluGlyGlyLys820825830LysGlnAlaGluAlaGlnThrGlyIleThrGlySerGlnLysLeuPro835840845HisGlyLeuGluThrAsnIleSerArgGluGluLeuLeuGluLeuGly850855860GlnAlaPheAlaAsnThrProGluGlyPheAsnTyrHisProArgVal865870875880AlaProValAlaLysLysArgValSerSerValThrGluGlyGlyIle885890895AspTrpAlaTrpGlyGluLeuLeuAlaPheGlySerLeuAlaAsnSer900905910GlyArgLeuValArgLeuAlaGlyGluAspSerArgArgGlyThrPhe915920925ThrGlnArgHisAlaValAlaIleAspProAlaThrAlaGluGluPhe930935940AsnProLeuHisGluLeuAlaGlnSerLysGlyAsnAsnGlyLysPhe945950955960LeuValTyrAsnSerAlaLeuThrGluTyrAlaGlyMetGlyPheGlu965970975TyrGlyTyrSerValGlyAsnGluAspSerIleValAlaTrpGluAla980985990GlnPheGlyAspPheAlaAsnGlyAlaGlnThrIleIleAspGluTyr99510001005ValSerSerGlyGluAlaLysTrpGlyGlnThrSerLysLeuIleLeu101010151020LeuLeuProHisGlyTyrGluGlyGlnGlyProAspHisSerSerAla1025103010351040ArgIleGluArgPheLeuGlnLeuCysAlaGluGlySerMetThrVal104510501055AlaGlnProSerThrProAlaAsnHisPheHisLeuLeuArgArgHis106010651070AlaLeuSerAspLeuLysArgProLeuValIlePheThrProLysSer107510801085MetLeuArgAsnLysAlaAlaAlaSerAlaProGluAspPheThrGlu109010951100ValThrLysPheGlnSerValIleAsnAspProAsnValAlaAspAla1105111011151120AlaLysValLysLysValMetLeuValSerGlyLysLeuTyrTyrGlu112511301135LeuAlaLysArgLysGluLysAspGlyArgAspAspIleAlaIleVal114011451150ArgIleGluMetLeuHisProIleProPheAsnArgIleSerGluAla115511601165LeuAlaGlyTyrProAsnAlaGluGluValLeuPheValGlnAspGlu117011751180ProAlaAsnGlnGlyProTrpProPheTyrGlnGluHisLeuProGlu1185119011951200LeuIleProAsnMetProLysMetArgArgValSerArgArgAlaGln120512101215SerSerThrAlaThrGlyValAlaLysValHisGlnLeuGluGluLys122012251230GlnLeuIleAspGluAlaPheGluAla12351240<210>31<211>3771<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3771)<223>RXA02056K<400>31atgctacaactggggcttaggcataatcagccaacgaccaacgttaca48MetLeuGlnLeuGlyLeuArgHisAsnGlnProThrThrAsnValThr151015gtggataaaacaaagctcaataaaccctcaagaagcaaggaaaagagg96ValAspLysThrLysLeuAsnLysProSerArgSerLysGluLysArg202530cgagtacctgccgtgagcagcgctagtactttcggccagaatgcgtgg144ArgValProAlaValSerSerAlaSerThrPheGlyGlnAsnAlaTrp354045ctggtagacgagatgttccagcagttccagaaggaccccaagtccgtg192LeuValAspGluMetPheGlnGlnPheGlnLysAspProLysSerVal505560gacaaggaatggagagaactctttgaggcgcaggggggaccaaatact240AspLysGluTrpArgGluLeuPheGluAlaGlnGlyGlyProAsnThr65707580acccccgctacaacagaagcacagccttcagcgcccaaggagtctgcg288ThrProAlaThrThrGluAlaGlnProSerAlaProLysGluSerAla859095aaaccagcaccaaaggctgcccctgcagccaaggcagcaccgcgcgta336LysProAlaProLysAlaAlaProAlaAlaLysAlaAlaProArgVal100105110gaaaccaagccggccgacaagaccgcccctaaggccaaggagtcctca384GluThrLysProAlaAspLysThrAlaProLysAlaLysGluSerSer115120125gtgccacagcaacctaagcttccggagccaggacaaaccccaatcagg432ValProGlnGlnProLysLeuProGluProGlyGlnThrProIleArg130135140ggtattttcaagtccatcgcgaagaacatggatatctccctggaaatc480GlyIlePheLysSerIleAlaLysAsnMetAspIleSerLeuGluIle145150155160ccaaccgcaacctcggttcgcgatatgccagctcgcctcatgttcgaa528ProThrAlaThrSerValArgAspMetProAlaArgLeuMetPheGlu165170175aaccgcgcgatggtcaacgatcagctcaagcgcacccgcggtggcaag576AsnArgAlaMetValAsnAspGlnLeuLysArgThrArgGlyGlyLys180185190atctccttcacccacatcattggctacgccatggtgaaggcagtcatg624IleSerPheThrHisIleIleGlyTyrAlaMetValLysAlaValMet195200205gctcacccggacatgaacaactcctacgacgtcatcgacggcaagcca672AlaHisProAspMetAsnAsnSerTyrAspValIleAspGlyLysPro210215220accctgatcgtgcctgagcacatcaacctgggccttgctatcgacctt720ThrLeuIleValProGluHisIleAsnLeuGlyLeuAlaIleAspLeu225230235240cctcagaaggacggctcccgcgcacttgtcgtagcagccatcaaggaa768ProGlnLysAspGlySerArgAlaLeuValValAlaAlaIleLysGlu245250255accgagaagatgaacttctccgagttcctcgcagcctacgaagacatc816ThrGluLysMetAsnPheSerGluPheLeuAlaAlaTyrGluAspIle260265270gtggcacgctcccgcaagggcaagctcaccatggatgactaccagggc864ValAlaArgSerArgLysGlyLysLeuThrMetAspAspTyrGlnGly275280285gttaccgtttccttgaccaacccaggtggcatcggtacccgccactct912ValThrValSerLeuThrAsnProGlyGlyIleGlyThrArgHisSer290295300gttccacgtctaaccaagggccagggcaccatcatcggtgtcggttcc960ValProArgLeuThrLysGlyGlnGlyThrIleIleGlyValGlySer305310315320atggattacccagcagagttccagggcgcttcagaagaccgccttgca1008MetAspTyrProAlaGluPheGlnGlyAlaSerGluAspArgLeuAla325330335gagctcggcgttggcaaacttgtcaccatcacctccacctacgatcac1056GluLeuGlyValGlyLysLeuValThrIleThrSerThrTyrAspHis340345350cgcgtgatccagggtgctgtgtccggtgaattcctgcgcaccatgtct1104ArgValIleGlnGlyAlaValSerGlyGluPheLeuArgThrMetSer355360365cgcctgctcaccgatgattccttctgggatgagatcttcgacgcaatg1152ArgLeuLeuThrAspAspSerPheTrpAspGluIlePheAspAlaMet370375380aacgttccttacaccccaatgcgttgggcacaggacgttccaaacacc1200AsnValProTyrThrProMetArgTrpAlaGlnAspValProAsnThr385390395400ggtgttgataagaacacccgcgtcatgcagctcattgaggcataccgc1248GlyValAspLysAsnThrArgValMetGlnLeuIleGluAlaTyrArg405410415tcccgtggacacctcatcgctgacaccaacccactttcatgggttcag1296SerArgGlyHisLeuIleAlaAspThrAsnProLeuSerTrpValGln420425430cctggcatgccagttccagaccaccgcgacctcgacatcgagacccac1344ProGlyMetProValProAspHisArgAspLeuAspIleGluThrHis435440445aacctgaccatctgggatctggaccgtaccttcaacgtcggtggcttc1392AsnLeuThrIleTrpAspLeuAspArgThrPheAsnValGlyGlyPhe450455460ggcggcaaggagaccatgaccctgcgcgaggtactgtcccgcctccgc1440GlyGlyLysGluThrMetThrLeuArgGluValLeuSerArgLeuArg465470475480gctgcgtacaccctcaaggtcggctccgaatacacccacatcctggac1488AlaAlaTyrThrLeuLysValGlySerGluTyrThrHisIleLeuAsp485490495cgcgacgagcgcacctggctgcaggaccgcctcgaggccggaatgcca1536ArgAspGluArgThrTrpLeuGlnAspArgLeuGluAlaGlyMetPro500505510aagccaacccaggcagagcagaagtacatcctgcagaagctgaacgcc1584LysProThrGlnAlaGluGlnLysTyrIleLeuGlnLysLeuAsnAla515520525gcggaggctttcgagaacttcctgcagaccaagtacgtcggccagaag1632AlaGluAlaPheGluAsnPheLeuGlnThrLysTyrValGlyGlnLys530535540cgcttctccctcgaaggtgcagaagcacttatcccactgatggactcc1680ArgPheSerLeuGluGlyAlaGluAlaLeuIleProLeuMetAspSer545550555560gccatcgacaccgccgcaggccaaggcctcgacgaagttgtcatcggt1728AlaIleAspThrAlaAlaGlyGlnGlyLeuAspGluValValIleGly565570575atgccacaccgtggtcgcctcaacgtgctgttcaacatcgtgggcaag1776MetProHisArgGlyArgLeuAsnValLeuPheAsnIleValGlyLys580585590ccactggcatccatcttcaacgagtttgaaggccaaatggagcagggc1824ProLeuAlaSerIlePheAsnGluPheGluGlyGlnMetGluGlnGly595600605cagatcggtggctccggtgacgtgaagtaccacctcggttccgaaggc1872GlnIleGlyGlySerGlyAspValLysTyrHisLeuGlySerGluGly610615620cagcacctgcagatgttcggcgacggcgagatcaaggtctccctgact1920GlnHisLeuGlnMetPheGlyAspGlyGluIleLysValSerLeuThr625630635640gctaacccgtcccacctggaagctgttaacccagtgatggaaggtatc1968AlaAsnProSerHisLeuGluAlaValAsnProValMetGluGlyIle645650655gtccgcgcaaagcaggactacctggacaagggcgtagacggcaagact2016ValArgAlaLysGlnAspTyrLeuAspLysGlyValAspGlyLysThr660665670gttgtgccactgctgctccacggtgacgctgcattcgcaggcctgggc2064ValValProLeuLeuLeuHisGlyAspAlaAlaPheAlaGlyLeuGly675680685atcgtgccagaaaccatcaacctggctaagctgcgtggctacgacgtc2112IleValProGluThrIleAsnLeuAlaLysLeuArgGlyTyrAspVal690695700ggcggcaccatccacatcgtggtgaacaaccagatcggcttcaccacc2160GlyGlyThrIleHisIleValValAsnAsnGlnIleGlyPheThrThr705710715720accccagactccagccgctccatgcactacgcaaccgactacgccaag2208ThrProAspSerSerArgSerMetHisTyrAlaThrAspTyrAlaLys725730735gcattcggctgcccagtcttccacgtcaacggcgacgacccagaggca2256AlaPheGlyCysProValPheHisValAsnGlyAspAspProGluAla740745750gttgtctgggttggccagctggccaccgagtaccgtcgtcgcttcggc2304ValValTrpValGlyGlnLeuAlaThrGluTyrArgArgArgPheGly755760765aaggacgtcttcatcgacctcgtctgctaccgcctccgcggccacaac2352LysAspValPheIleAspLeuValCysTyrArgLeuArgGlyHisAsn770775780gaagctgatgatccttccatgacccagccaaagatgtatgagctcatc2400GluAlaAspAspProSerMetThrGlnProLysMetTyrGluLeuIle785790795800accggccgcgagaccgttcgtgctcagtacaccgaagacctgctcgga2448ThrGlyArgGluThrValArgAlaGlnTyrThrGluAspLeuLeuGly805810815cgtggagacctctccaacgaagatgcagaagcagtcgtccgcgacttc2496ArgGlyAspLeuSerAsnGluAspAlaGluAlaValValArgAspPhe820825830cacgaccagatggaatctgtgttcaacgaagtcaaggaaggcggcaag2544HisAspGlnMetGluSerValPheAsnGluValLysGluGlyGlyLys835840845aagcaggctgaggcacagaccggcatcaccggctcccagaagcttcca2592LysGlnAlaGluAlaGlnThrGlyIleThrGlySerGlnLysLeuPro850855860cacggccttgagaccaacatctcccgtgaagagctcctggaactggga2640HisGlyLeuGluThrAsnIleSerArgGluGluLeuLeuGluLeuGly865870875880caggctttcgccaacaccccagaaggcttcaactaccacccacgtgtg2688GlnAlaPheAlaAsnThrProGluGlyPheAsnTyrHisProArgVal885890895gctcccgttgctaagaagcgcgtctcctctgtcaccgaaggtggcatc2736AlaProValAlaLysLysArgValSerSerValThrGluGlyGlyIle900905910gactgggcatggggcgagctcctcgccttcggttccctggctaactcc2784AspTrpAlaTrpGlyGluLeuLeuAlaPheGlySerLeuAlaAsnSer915920925ggccgcttggttcgccttgcaggtgaagattcccgccgcggtaccttc2832GlyArgLeuValArgLeuAlaGlyGluAspSerArgArgGlyThrPhe930935940acccagcgccacgcagttgccatcgacccagcgaccgctgaagagttc2880ThrGlnArgHisAlaValAlaIleAspProAlaThrAlaGluGluPhe945950955960aacccactccacgagcttgcacagtccaagggcaacaacggtaagttc2928AsnProLeuHisGluLeuAlaGlnSerLysGlyAsnAsnGlyLysPhe965970975ctggtctacaactccgcactgaccgagtacgcaggcatgggcttcgag2976LeuValTyrAsnSerAlaLeuThrGluTyrAlaGlyMetGlyPheGlu980985990tacggctactccgtaggaaacgaagactccatcgttgcatgggaagca3024TyrGlyTyrSerValGlyAsnGluAspSerIleValAlaTrpGluAla99510001005cagttcggcgacttcgccaacggcgctcagaccatcatcgatgagtac3072GlnPheGlyAspPheAlaAsnGlyAlaGlnThrIleIleAspGluTyr101010151020gtctcctcaggcgaagctaagtggggccagacctccaagctgatcctt3120ValSerSerGlyGluAlaLysTrpGlyGlnThrSerLysLeuIleLeu1025103010351040ctgctgcctcacggctacgaaggccagggcccagaccactcttccgca3168LeuLeuProHisGlyTyrGluGlyGlnGlyProAspHisSerSerAla104510501055cgtatcgagcgcttcctgcagctgtgcgctgagggttccatgactgtt3216ArgIleGluArgPheLeuGlnLeuCysAlaGluGlySerMetThrVal106010651070gctcagccatccaccccagcaaaccacttccacctactgcgtcgtcac3264AlaGlnProSerThrProAlaAsnHisPheHisLeuLeuArgArgHis107510801085gctctgtccgacctgaagcgtccactggttatcttcaccccgaagtcc3312AlaLeuSerAspLeuLysArgProLeuValIlePheThrProLysSer109010951100atgctgcgtaacaaggctgctgcctccgcaccagaagacttcactgag3360MetLeuArgAsnLysAlaAlaAlaSerAlaProGluAspPheThrGlu1105111011151120gtcaccaagttccagtccgtgatcaacgatccaaacgttgcagatgca3408ValThrLysPheGlnSerValIleAsnAspProAsnValAlaAspAla112511301135gccaaggtgaagaaggtcatgctggtctccggcaagctgtactacgaa3456AlaLysValLysLysValMetLeuValSerGlyLysLeuTyrTyrGlu114011451150ttggcaaagcgcaaggagaaggacggacgcgacgacatcgcgatcgtt3504LeuAlaLysArgLysGluLysAspGlyArgAspAspIleAlaIleVal115511601165cgtatcgaaatgctccacccaattccgttcaaccgcatctccgaggct3552ArgIleGluMetLeuHisProIleProPheAsnArgIleSerGluAla117011751180cttgccggctaccctaacgctgaggaagtcctcttcgttcaggatgag3600LeuAlaGlyTyrProAsnAlaGluGluValLeuPheValGlnAspGlu1185119011951200ccagcaaaccagggcccatggccgttctaccaggagcacctcccagag3648ProAlaAsnGlnGlyProTrpProPheTyrGlnGluHisLeuProGlu120512101215ctgatcccgaacatgccaaagatgcgccgcgtttcccgccgcgctcag3696LeuIleProAsnMetProLysMetArgArgValSerArgArgAlaGln122012251230tcctccaccgcaactggtgttgccaaggtgcaccagctggaggagaag3744SerSerThrAlaThrGlyValAlaLysValHisGlnLeuGluGluLys123512401245cagcttatcgacgaggctttcgaggct3771GlnLeuIleAspGluAlaPheGluAla12501255<210>32<211>1257<212>PRT<213>谷氨酸棒杆菌<400>32MetLeuGlnLeuGlyLeuArgHisAsnGlnProThrThrAsnValThr151015ValAspLysThrLysLeuAsnLysProSerArgSerLysGluLysArg202530ArgValProAlaValSerSerAlaSerThrPheGlyGlnAsnAlaTrp354045LeuValAspGluMetPheGlnGlnPheGlnLysAspProLysSerVal505560AspLysGluTrpArgGluLeuPheGluAlaGlnGlyGlyProAsnThr65707580ThrProAlaThrThrGluAlaGlnProSerAlaProLysGluSerAla859095LysProAlaProLysAlaAlaProAlaAlaLysAlaAlaProArgVal100105110GluThrLysProAlaAspLysThrAlaProLysAlaLysGluSerSer115120125ValProGlnGlnProLysLeuProGluProGlyGlnThrProIleArg130135140GlyIlePheLysSerIleAlaLysAsnMetAspIleSerLeuGluIle145150155160ProThrAlaThrSerValArgAspMetProAlaArgLeuMetPheGlu165170175AsnArgAlaMetValAsnAspGlnLeuLysArgThrArgGlyGlyLys180185190IleSerPheThrHisIleIleGlyTyrAlaMetValLysAlaValMet195200205AlaHisProAspMetAsnAsnSerTyrAspValIleAspGlyLysPro210215220ThrLeuIleValProGluHisIleAsnLeuGlyLeuAlaIleAspLeu225230235240ProGlnLysAspGlySerArgAlaLeuValValAlaAlaIleLysGlu245250255ThrGluLysMetAsnPheSerGluPheLeuAlaAlaTyrGluAspIle260265270ValAlaArgSerArgLysGlyLysLeuThrMetAspAspTyrGlnGly275280285ValThrValSerLeuThrAsnProGlyGlyIleGlyThrArgHisSer290295300ValProArgLeuThrLysGlyGlnGlyThrIleIleGlyValGlySer305310315320MetAspTyrProAlaGluPheGlnGlyAlaSerGluAspArgLeuAla325330335GluLeuGlyValGlyLysLeuValThrIleThrSerThrTyrAspHis340345350ArgValIleGlnGlyAlaValSerGlyGluPheLeuArgThrMetSer355360365ArgLeuLeuThrAspAspSerPheTrpAspGluIlePheAspAlaMet370375380AsnValProTyrThrProMetArgTrpAlaGlnAspValProAsnThr385390395400GlyValAspLysAsnThrArgValMetGlnLeuIleGluAlaTyrArg405410415SerArgGlyHisLeuIleAlaAspThrAsnProLeuSerTrpValGln420425430ProGlyMetProValProAspHisArgAspLeuAspIleGluThrHis435440445AsnLeuThrIleTrpAspLeuAspArgThrPheAsnValGlyGlyPhe450455460GlyGlyLysGluThrMetThrLeuArgGluValLeuSerArgLeuArg465470475480AlaAlaTyrThrLeuLysValGlySerGluTyrThrHisIleLeuAsp485490495ArgAspGluArgThrTrpLeuGlnAspArgLeuGluAlaGlyMetPro500505510LysProThrGlnAlaGluGlnLysTyrIleLeuGlnLysLeuAsnAla515520525AlaGluAlaPheGluAsnPheLeuGlnThrLysTyrValGlyGlnLys530535540ArgPheSerLeuGluGlyAlaGluAlaLeuIleProLeuMetAspSer545550555560AlaIleAspThrAlaAlaGlyGlnGlyLeuAspGluValValIleGly565570575MetProHisArgGlyArgLeuAsnValLeuPheAsnIleValGlyLys580585590ProLeuAlaSerIlePheAsnGluPheGluGlyGlnMetGluGlnGly595600605GlnIleGlyGlySerGlyAspValLysTyrHisLeuGlySerGluGly610615620GlnHisLeuGlnMetPheGlyAspGlyGluIleLysValSerLeuThr625630635640AlaAsnProSerHisLeuGluAlaValAsnProValMetGluGlyIle645650655ValArgAlaLysGlnAspTyrLeuAspLysGlyValAspGlyLysThr660665670ValValProLeuLeuLeuHisGlyAspAlaAlaPheAlaGlyLeuGly675680685IleValProGluThrIleAsnLeuAlaLysLeuArgGlyTyrAspVal690695700GlyGlyThrIleHisIleValValAsnAsnGlnIleGlyPheThrThr705710715720ThrProAspSerSerArgSerMetHisTyrAlaThrAspTyrAlaLys725730735AlaPheGlyCysProValPheHisValAsnGlyAspAspProGluAla740745750ValValTrpValGlyGlnLeuAlaThrGluTyrArgArgArgPheGly755760765LysAspValPheIleAspLeuValCysTyrArgLeuArgGlyHisAsn770775780GluAlaAspAspProSerMetThrGlnProLysMetTyrGluLeuIle785790795800ThrGlyArgGluThrValArgAlaGlnTyrThrGluAspLeuLeuGly805810815ArgGlyAspLeuSerAsnGluAspAlaGluAlaValValArgAspPhe820825830HisAspGlnMetGluSerValPheAsnGluValLysGluGlyGlyLys835840845LysGlnAlaGluAlaGlnThrGlyIleThrGlySerGlnLysLeuPro850855860HisGlyLeuGluThrAsnIleSerArgGluGluLeuLeuGluLeuGly865870875880GlnAlaPheAlaAsnThrProGluGlyPheAsnTyrHisProArgVal885890895AlaProValAlaLysLysArgValSerSerValThrGluGlyGlyIle900905910AspTrpAlaTrpGlyGluLeuLeuAlaPheGlySerLeuAlaAsnSer915920925GlyArgLeuValArgLeuAlaGlyGluAspSerArgArgGlyThrPhe930935940ThrGlnArgHisAlaValAlaIleAspProAlaThrAlaGluGluPhe945950955960AsnProLeuHisGluLeuAlaGlnSerLysGlyAsnAsnGlyLysPhe965970975LeuValTyrAsnSerAlaLeuThrGluTyrAlaGlyMetGlyPheGlu980985990TyrGlyTyrSerValGlyAsnGluAspSerIleValAlaTrpGluAla99510001005GlnPheGlyAspPheAlaAsnGlyAlaGlnThrIleIleAspGluTyr101010151020ValSerSerGlyGluAlaLysTrpGlyGlnThrSerLysLeuIleLeu1025103010351040LeuLeuProHisGlyTyrGluGlyGlnGlyProAspHisSerSerAla104510501055ArgIleGluArgPheLeuGlnLeuCysAlaGluGlySerMetThrVal106010651070AlaGlnProSerThrProAlaAsnHisPheHisLeuLeuArgArgHis107510801085AlaLeuSerAspLeuLysArgProLeuValIlePheThrProLysSer109010951100MetLeuArgAsnLysAlaAlaAlaSerAlaProGluAspPheThrGlu1105111011151120ValThrLysPheGlnSerValIleAsnAspProAsnValAlaAspAla112511301135AlaLysValLysLysValMetLeuValSerGlyLysLeuTyrTyrGlu114011451150LeuAlaLysArgLysGluLysAspGlyArgAspAspIleAlaIleVal115511601165ArgIleGluMetLeuHisProIleProPheAsnArgIleSerGluAla117011751180LeuAlaGlyTyrProAsnAlaGluGluValLeuPheValGlnAspGlu1185119011951200ProAlaAsnGlnGlyProTrpProPheTyrGlnGluHisLeuProGlu120512101215LeuIleProAsnMetProLysMetArgArgValSerArgArgAlaGln122012251230SerSerThrAlaThrGlyValAlaLysValHisGlnLeuGluGluLys123512401245GlnLeuIleAspGluAlaPheGluAla12501255<210>33<211>1251<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1251)<223>RXA02063E<400>33atgcgggatagttattttgcctttatggttaagggtgtgaagggtaga48MetArgAspSerTyrPheAlaPheMetValLysGlyValLysGlyArg151015ccaaatgttctagcaatcgttctcgcaggtggcgagggcaaacgactt96ProAsnValLeuAlaIleValLeuAlaGlyGlyGluGlyLysArgLeu202530tttccgttgacggaggaccgagctaagcctgcggtcccattcggcgga144PheProLeuThrGluAspArgAlaLysProAlaValProPheGlyGly354045acttacagattgatcgactttgttttgtcgaacctggtgaactccgga192ThrTyrArgLeuIleAspPheValLeuSerAsnLeuValAsnSerGly505560ttcctcaagatcgcggtactgactcagtacaagtcgcattcattggat240PheLeuLysIleAlaValLeuThrGlnTyrLysSerHisSerLeuAsp65707580aggcatatttcattgtcgtggaacgtgtctggtccaacggggcagtac288ArgHisIleSerLeuSerTrpAsnValSerGlyProThrGlyGlnTyr859095attgcttctgttcctgcgcagcagcgcctgggcaagcgatggttcact336IleAlaSerValProAlaGlnGlnArgLeuGlyLysArgTrpPheThr100105110ggttccgcggatgcaattttgcagtctctgaacttgatctctgatgag384GlySerAlaAspAlaIleLeuGlnSerLeuAsnLeuIleSerAspGlu115120125aaaccggattatgtcatcgttttcggcgcggaccacgtgtatcgcatg432LysProAspTyrValIleValPheGlyAlaAspHisValTyrArgMet130135140gacccaagccagatgctagatgagcacattgcatctggtcgcgcggtg480AspProSerGlnMetLeuAspGluHisIleAlaSerGlyArgAlaVal145150155160tctgtggcaggtattcgcgttccacgtgaggaagcaactgcgtttggt528SerValAlaGlyIleArgValProArgGluGluAlaThrAlaPheGly165170175tgcatccagtccgatgtcgacggcaacataaccgagttcttggaaaag576CysIleGlnSerAspValAspGlyAsnIleThrGluPheLeuGluLys180185190ccagctgaccctccgggaacccctgatgatcctgacatgacttacgcg624ProAlaAspProProGlyThrProAspAspProAspMetThrTyrAla195200205tcgatgggtaactacattttcaccactgaagccctgatccaggcgctg672SerMetGlyAsnTyrIlePheThrThrGluAlaLeuIleGlnAlaLeu210215220aaagatgatgaaaataacgagaacagtgatcatgacatgggcggagac720LysAspAspGluAsnAsnGluAsnSerAspHisAspMetGlyGlyAsp225230235240atcattccgtacttcgtctctcgcaatgatgcgcatgtttatgatttc768IleIleProTyrPheValSerArgAsnAspAlaHisValTyrAspPhe245250255tccggaaacattgttcctggcgcaactgagcgtgacaagggctactgg816SerGlyAsnIleValProGlyAlaThrGluArgAspLysGlyTyrTrp260265270cgcgacgtcggtaccattgatgcgttctacgagtgccacatggacctg864ArgAspValGlyThrIleAspAlaPheTyrGluCysHisMetAspLeu275280285atttccgtgcacccaatcttcaatctgtataactctgagtggccaatc912IleSerValHisProIlePheAsnLeuTyrAsnSerGluTrpProIle290295300cacacgacctctgaaggtaacttgcctccggctaagttcgttcggggc960HisThrThrSerGluGlyAsnLeuProProAlaLysPheValArgGly305310315320ggtatcgcgcagtcgtcgatggtgtcttcaggttccatcatttctgct1008GlyIleAlaGlnSerSerMetValSerSerGlySerIleIleSerAla325330335gggactgttcgcaactccgtgctgtccaacaacgttgtcgtcgaagag1056GlyThrValArgAsnSerValLeuSerAsnAsnValValValGluGlu340345350ggcgcaacggtggaaggtgcagtgctgatgccaggcgtgcgcatcggt1104GlyAlaThrValGluGlyAlaValLeuMetProGlyValArgIleGly355360365aagggtgctgttgtccgccatgcgattctggacaagaacgtggttgtc1152LysGlyAlaValValArgHisAlaIleLeuAspLysAsnValValVal370375380cgcgacggagagctcatcggtgtcgaccaagtgcgcgatgcgcagcgc1200ArgAspGlyGluLeuIleGlyValAspGlnValArgAspAlaGlnArg385390395400ttcaaggtgagcgccggcggcgtcgtggttgtcggtaagaaccaggta1248PheLysValSerAlaGlyGlyValValValValGlyLysAsnGlnVal405410415gtc1251Val<210>34<211>417<212>PRT<213>谷氨酸棒杆菌<400>34MetArgAspSerTyrPheAlaPheMetValLysGlyValLysGlyArg151015ProAsnValLeuAlaIleValLeuAlaGlyGlyGluGlyLysArgLeu202530PheProLeuThrGluAspArgAlaLysProAlaValProPheGlyGly354045ThrTyrArgLeuIleAspPheValLeuSerAsnLeuValAsnSerGly505560PheLeuLysIleAlaValLeuThrGlnTyrLysSerHisSerLeuAsp65707580ArgHisIleSerLeuSerTrpAsnValSerGlyProThrGlyGlnTyr859095IleAlaSerValProAlaGlnGlnArgLeuGlyLysArgTrpPheThr100105110GlySerAlaAspAlaIleLeuGlnSerLeuAsnLeuIleSerAspGlu115120125LysProAspTyrValIleValPheGlyAlaAspHisValTyrArgMet130135140AspProSerGlnMetLeuAspGluHisIleAlaSerGlyArgAlaVal145150155160SerValAlaGlyIleArgValProArgGluGluAlaThrAlaPheGly165170175CysIleGlnSerAspValAspGlyAsnIleThrGluPheLeuGluLys180185190ProAlaAspProProGlyThrProAspAspProAspMetThrTyrAla195200205SerMetGlyAsnTyrIlePheThrThrGluAlaLeuIleGlnAlaLeu210215220LysAspAspGluAsnAsnGluAsnSerAspHisAspMetGlyGlyAsp225230235240IleIleProTyrPheValSerArgAsnAspAlaHisValTyrAspPhe245250255SerGlyAsnIleValProGlyAlaThrGluArgAspLysGlyTyrTrp260265270ArgAspValGlyThrIleAspAlaPheTyrGluCysHisMetAspLeu275280285IleSerValHisProIlePheAsnLeuTyrAsnSerGluTrpProIle290295300HisThrThrSerGluGlyAsnLeuProProAlaLysPheValArgGly305310315320GlyIleAlaGlnSerSerMetValSerSerGlySerIleIleSerAla325330335GlyThrValArgAsnSerValLeuSerAsnAsnValValValGluGlu340345350GlyAlaThrValGluGlyAlaValLeuMetProGlyValArgIleGly355360365LysGlyAlaValValArgHisAlaIleLeuAspLysAsnValValVal370375380ArgAspGlyGluLeuIleGlyValAspGlnValArgAspAlaGlnArg385390395400PheLysValSerAlaGlyGlyValValValValGlyLysAsnGlnVal405410415Val<210>35<211>1227<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1227)<223>RXA02063W<400>35atggttaagggtgtgaagggtagaccaaatgttctagcaatcgttctc48MetValLysGlyValLysGlyArgProAsnValLeuAlaIleValLeu151015gcaggtggcgagggcaaacgactttttccgttgacggaggaccgagct96AlaGlyGlyGluGlyLysArgLeuPheProLeuThrGluAspArgAla202530aagcctgcggtcccattcggcggaacttacagattgatcgactttgtt144LysProAlaValProPheGlyGlyThrTyrArgLeuIleAspPheVal354045ttgtcgaacctggtgaactccggattcctcaagatcgcggtactgact192LeuSerAsnLeuValAsnSerGlyPheLeuLysIleAlaValLeuThr505560cagtacaagtcgcattcattggataggcatatttcattgtcgtggaac240GlnTyrLysSerHisSerLeuAspArgHisIleSerLeuSerTrpAsn65707580gtgtctggtccaacggggcagtacattgcttctgttcctgcgcagcag288ValSerGlyProThrGlyGlnTyrIleAlaSerValProAlaGlnGln859095cgcctgggcaagcgatggttcactggttccgcggatgcaattttgcag336ArgLeuGlyLysArgTrpPheThrGlySerAlaAspAlaIleLeuGln100105110tctctgaacttgatctctgatgagaaaccggattatgtcatcgttttc384SerLeuAsnLeuIleSerAspGluLysProAspTyrValIleValPhell5120125ggcgcggaccacgtgtatcgcatggacccaagccagatgctagatgag432GlyAlaAspHisValTyrArgMetAspProSerGlnMetLeuAspGlu130135140cacattgcatctggtcgcgcggtgtctgtggcaggtattcgcgttcca480HisIleAlaSerGlyArgAlaValSerValAlaGlyIleArgValPro145150155160cgtgaggaagcaactgcgtttggttgcatccagtccgatgtcgacggc528ArgGluGluAlaThrAlaPheGlyCysIleGlnSerAspValAspGly165170175aacataaccgagttcttggaaaagccagctgaccctccgggaacccct576AsnIleThrGluPheLeuGluLysProAlaAspProProGlyThrPro180185190gatgatcctgacatgacttacgcgtcgatgggtaactacattttcacc624AspAspProAspMetThrTyrAlaSerMetGlyAsnTyrIlePheThr195200205actgaagccctgatccaggcgctgaaagatgatgaaaataacgagaac672ThrGluAlaLeuIleGlnAlaLeuLysAspAspGluAsnAsnGluAsn210215220agtgatcatgacatgggcggagacatcattccgtacttcgtctctcgc720SerAspHisAspMetGlyGlyAspIleIleProTyrPheValSerArg225230235240aatgatgcgcatgtttatgatttctccggaaacattgttcctggcgca768AsnAspAlaHisValTyrAspPheSerGlyAsnIleValProGlyAla245250255actgagcgtgacaagggctactggcgcgacgtcggtaccattgatgcg816ThrGluArgAspLysGlyTyrTrpArgAspValGlyThrIleAspAla260265270ttctacgagtgccacatggacctgatttccgtgcacccaatcttcaat864PheTyrGluCysHisMetAspLeuIleSerValHisProIlePheAsn275280285ctgtataactctgagtggccaatccacacgacctctgaaggtaacttg912LeuTyrAsnSerGluTrpProIleHisThrThrSerGluGlyAsnLeu290295300cctccggctaagttcgttcggggcggtatcgcgcagtcgtcgatggtg960ProProAlaLysPheValArgGlyGlyIleAlaGlnSerSerMetVal305310315320tcttcaggttccatcatttctgctgggactgttcgcaactccgtgctg1008SerSerGlySerIleIleSerAlaGlyThrValArgAsnSerValLeu325330335tccaacaacgttgtcgtcgaagagggcgcaacggtggaaggtgcagtg1056SerAsnAsnValValValGluGluGlyAlaThrValGluGlyAlaVal340345350ctgatgccaggcgtgcgcatcggtaagggtgctgttgtccgccatgcg1104LeuMetProGlyValArgIleGlyLysGlyAlaValValArgHisAla355360365attctggacaagaacgtggttgtccgcgacggagagctcatcggtgtc1152IleLeuAspLysAsnValValValArgAspGlyGluLeuIleGlyVal370375380gaccaagtgcgcgatgcgcagcgcttcaaggtgagcgccggcggcgtc1200AspGlnValArgAspAlaGlnArgPheLysValSerAlaGlyGlyVal385390395400gtggttgtcggtaagaaccaggtagtc1227ValValValGlyLysAsnGlnValVal405<210>36<211>409<212>PRT<213>谷氨酸棒杆菌<400>36MetValLysGlyValLysGlyArgProAsnValLeuAlaIleValLeu151015AlaGlyGlyGluGlyLysArgLeuPheProLeuThrGluAspArgAla202530LysProAlaValProPheGlyGlyThrTyrArgLeuIleAspPheVal354045LeuSerAsnLeuValAsnSerGlyPheLeuLysIleAlaValLeuThr505560GlnTyrLysSerHisSerLeuAspArgHisIleSerLeuSerTrpAsn65707580ValSerGlyProThrGlyGlnTyrIleAlaSerValProAlaGlnGln859095ArgLeuGlyLysArgTrpPheThrGlySerAlaAspAlaIleLeuGln100105110SerLeuAsnLeuIleSerAspGluLysProAspTyrValIleValPhe115120125GlyAlaAspHisValTyrArgMetAspProSerGlnMetLeuAspGlu130135140HisIleAlaSerGlyArgAlaValSerValAlaGlyIleArgValPro145150155160ArgGluGluAlaThrAlaPheGlyCysIleGlnSerAspValAspGly165170175AsnIleThrGluPheLeuGluLysProAlaAspProProGlyThrPro180185190AspAspProAspMetThrTyrAlaSerMetGlyAsnTyrIlePheThr195200205ThrGluAlaLeuIleGlnAlaLeuLysAspAspGluAsnAsnGluAsn210215220SerAspHisAspMetGlyGlyAspIleIleProTyrPheValSerArg225230235240AsnAspAlaHisValTyrAspPheSerGlyAsnIleValProGlyAla245250255ThrGluArgAspLysGlyTyrTrpArgAspValGlyThrIleAspAla260265270PheTyrGluCysHisMetAspLeuIleSerValHisProIlePheAsn275280285LeuTyrAsnSerGluTrpProIleHisThrThrSerGluGlyAsnLeu290295300ProProAlaLysPheValArgGlyGlyIleAlaGlnSerSerMetVal305310315320SerSerGlySerIleIleSerAlaGlyThrValArgAsnSerValLeu325330335SerAsnAsnValValValGluGluGlyAlaThrValGluGlyAlaVal340345350LeuMetProGlyValArgIleGlyLysGlyAlaValValArgHisAla355360365IleLeuAspLysAsnValValValArgAspGlyGluLeuIleGlyVal370375380AspGlnValArgAspAlaGlnArgPheLysValSerAlaGlyGlyVal385390395400ValValValGlyLysAsnGlnValVal405<210>37<211>3465<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3465)<223>RXA02082K<400>37atgtatttgaaatcgttgacgctcaaggggtttaagtctttcgcgtct48MetTyrLeuLysSerLeuThrLeuLysGlyPheLysSerPheAlaSer151015gcgacgaccctgaaatttgagccaggcatttgtgccgtggtgggtccg96AlaThrThrLeuLysPheGluProGlyIleCysAlaValValGlyPro202530aatggttcaggcaaatccaatgtggttgatgcgctggcctgggtgatg144AsnGlySerGlyLysSerAsnValValAspAlaLeuAlaTrpValMet354045ggtgaaggttctgcgaagaccttgcgtggcggcaaaatggaagatgtc192GlyGluGlySerAlaLysThrLeuArgGlyGlyLysMetGluAspVal505560atttttgctggcgcgggcgatcgtaaaccgttgggtcgcgcagaagtc240IlePheAlaGlyAlaGlyAspArgLysProLeuGlyArgAlaGluVal65707580acgctgaccattgataactctgatggcgcactgcccattgagtacacc288ThrLeuThrIleAspAsnSerAspGlyAlaLeuProIleGluTyrThr859095gaagtgtcggtgaccagacggatgttccgtgatggtgcaagtgaatat336GluValSerValThrArgArgMetPheArgAspGlyAlaSerGluTyr100105110gagatcaatggggcgaaagctcgattgatggatatccaggagctgttg384GluIleAsnGlyAlaLysAlaArgLeuMetAspIleGlnGluLeuLeu115120125tcggataccggtattggccgtgaaatgcacatcatggtggggcaggga432SerAspThrGlyIleGlyArgGluMetHisIleMetValGlyGlnGly130135140aagctcgcagagattttggagtcccgccccgaagagcgccgagcgtat480LysLeuAlaGluIleLeuGluSerArgProGluGluArgArgAlaTyr145150155160atcgaagaagctgcgggtgtgctcaagcaccggcgcaggaaagaaaag528IleGluGluAlaAlaGlyValLeuLysHisArgArgArgLysGluLys165170175gcgcagcgcaaacttcagggcatgcaggtcaatcttgatcgtttgcag576AlaGlnArgLysLeuGlnGlyMetGlnValAsnLeuAspArgLeuGln180185190gatctgacccatgagttggccaagcagctcaagccgttggctaggcag624AspLeuThrHisGluLeuAlaLysGlnLeuLysProLeuAlaArgGln195200205gcggaagcagcgcagcgtgcggcgacggtgcaggctgatttgcgtgat672AlaGluAlaAlaGlnArgAlaAlaThrValGlnAlaAspLeuArgAsp210215220gcgcgtttccagattgctggctttgagatcgtgaagctctcggaaaag720AlaArgPheGlnIleAlaGlyPheGluIleValLysLeuSerGluLys225230235240ctggaaacctctactgagcgcgagaaaatgattcgtgagcaggcggaa768LeuGluThrSerThrGluArgGluLysMetIleArgGluGlnAlaGlu245250255gcagcacaagagcagctggaagaagccaccacaactcagatggaagtg816AlaAlaGlnGluGlnLeuGluGluAlaThrThrThrGlnMetGluVal260265270gagatggagttggcggagatcactccgcaggctgaagctgcgcaacag864GluMetGluLeuAlaGluIleThrProGlnAlaGluAlaAlaGlnGln275280285ttgtggtttgatttgtcttcgctggctgagcgggtgtcggcaacgatg912LeuTrpPheAspLeuSerSerLeuAlaGluArgValSerAlaThrMet290295300cgtattgctgcagaccgtgcgagttcaggtgccgcggatgtgccgtat960ArgIleAlaAlaAspArgAlaSerSerGlyAlaAlaAspValProTyr305310315320gcgggccaggatcctgatgagttgcttggtcgggccgaaactgctgac1008AlaGlyGlnAspProAspGluLeuLeuGlyArgAlaGluThrAlaAsp325330335aaagaattagaagaactcgagatggccgtggaaatgaccaccgagcgt1056LysGluLeuGluGluLeuGluMetAlaValGluMetThrThrGluArg340345350ttgacctccattcaagaggaagccgaggataaggccgcgcaggctcgt1104LeuThrSerIleGlnGluGluAlaGluAspLysAlaAlaGlnAlaArg355360365gaggctgagcgtgaacacttggcgcaggtcagggcgatttctgatcgt1152GluAlaGluArgGluHisLeuAlaGlnValArgAlaIleSerAspArg370375380cgtgaaggtgttgtgcgcctgcttgcatctgaggaatctttgcgcacc1200ArgGluGlyValValArgLeuLeuAlaSerGluGluSerLeuArgThr385390395400cagcacacgtcagcagaggaggaagctgagcgactcagtgagcagctt1248GlnHisThrSerAlaGluGluGluAlaGluArgLeuSerGluGlnLeu405410415gaggagttcatcggccgcattttggatgtggaacgtgaacgtcgcctc1296GluGluPheIleGlyArgIleLeuAspValGluArgGluArgArgLeu420425430accgatgagcgtaaacagggcgttgacacggatcgtgcgcccttggaa1344ThrAspGluArgLysGlnGlyValAspThrAspArgAlaProLeuGlu435440445gaagccctcaaacaggcaaaacatgaagccgaagcagcagagactcgt1392GluAlaLeuLysGlnAlaLysHisGluAlaGluAlaAlaGluThrArg450455460cttgaggagcttcgtactaagcgcagcgatctggaaaaagaagtatcc1440LeuGluGluLeuArgThrLysArgSerAspLeuGluLysGluValSer465470475480aggttgcagtcgcgcattgagacgcttaaccaaaataggccacgttcc1488ArgLeuGlnSerArgIleGluThrLeuAsnGlnAsnArgProArgSer485490495gatgctgctgatgtggtggattacccgcagctggccacgttgattcga1536AspAlaAlaAspValValAspTyrProGlnLeuAlaThrLeuIleArg500505510ccgcaacgaaacgtcgataaggctctcgctgccgccctgggtgcgcat1584ProGlnArgAsnValAspLysAlaLeuAlaAlaAlaLeuGlyAlaHis515520525gccgaggcgctggctggcgaggctgcggaagggctcgtcgagaagctt1632AlaGluAlaLeuAlaGlyGluAlaAlaGluGlyLeuValGluLysLeu530535540atcgacgccggcgttgcacgcaccatcatcgttgatggcacgcaggct1680IleAspAlaGlyValAlaArgThrIleIleValAspGlyThrGlnAla545550555560ggcggcgcatggcgcctggacgcgaacattccggccggggcgagctgg1728GlyGlyAlaTrpArgLeuAspAlaAsnIleProAlaGlyAlaSerTrp565570575ctgctcgaccatgttgatctggatccggcgattgccggcccggtaaac1776LeuLeuAspHisValAspLeuAspProAlaIleAlaGlyProValAsn580585590cggctgcttgccgacgttgtgcttgtcgacgacccctccctcggccgc1824ArgLeuLeuAlaAspValValLeuValAspAspProSerLeuGlyArg595600605caagcaatcgaggatgatccccgtctgcgtgccgttgaccgcaatggt1872GlnAlaIleGluAspAspProArgLeuArgAlaValAspArgAsnGly610615620gtgctcatcggcgctgggtggattcaggtcggcaccgaaacctcgact1920ValLeuIleGlyAlaGlyTrpIleGlnValGlyThrGluThrSerThr625630635640gtggaaatcacagctcaaattgaggaagcagaagctcaacttgctgcg1968ValGluIleThrAlaGlnIleGluGluAlaGluAlaGlnLeuAlaAla645650655gcctctgccgccttggacgacattgccggcacttttgatggcgccctc2016AlaSerAlaAlaLeuAspAspIleAlaGlyThrPheAspGlyAlaLeu660665670cacgctgccgacaacactcgcgtcgaggtggctgcccgcaccgcagcc2064HisAlaAlaAspAsnThrArgValGluValAlaAlaArgThrAlaAla675680685ctgcgcgaactcgacatgaccagggattccatcacccgcgatctcgcg2112LeuArgGluLeuAspMetThrArgAspSerIleThrArgAspLeuAla690695700cgcttggacaaacaacatgaggccgccgaatccgagcgcgtccgccat2160ArgLeuAspLysGlnHisGluAlaAlaGluSerGluArgValArgHis705710715720gttggacgcctgcatgctgcggaaacacgccgtgaagagctgcgcgaa2208ValGlyArgLeuHisAlaAlaGluThrArgArgGluGluLeuArgGlu725730735cagttagaagacatcgtcgatcgactctcccgcgtggaagacgaagaa2256GlnLeuGluAspIleValAspArgLeuSerArgValGluAspGluGlu740745750gacgctgacgaaccctcaaccaccgcccgcgaccaagcaaatgccgag2304AspAlaAspGluProSerThrThrAlaArgAspGlnAlaAsnAlaGlu755760765ctgcaacaaatccgcgccatggaaatggaagcacgccttgcccaacgc2352LeuGlnGlnIleArgAlaMetGluMetGluAlaArgLeuAlaGlnArg770775780accgccgaagagcgcgccgggcagcagcggggcaagggcgatagtctg2400ThrAlaGluGluArgAlaGlyGlnGlnArgGlyLysGlyAspSerLeu785790795800cgacgccaggccgagcatgagcgccaagccaaaatccggcatgaacaa2448ArgArgGlnAlaGluHisGluArgGlnAlaLysIleArgHisGluGln805810815gccatggaagcccgtcgcaggcgcacccaattggctgcagccgtgcaa2496AlaMetGluAlaArgArgArgArgThrGlnLeuAlaAlaAlaValGln820825830aatggcgcacgcgatgtggccgagcgtgtctcagctgcccttgcccaa2544AsnGlyAlaArgAspValAlaGluArgValSerAlaAlaLeuAlaGln835840845gcagccatcgaaagagatcagcacaaccgcgacaaagcgctgctgacc2592AlaAlaIleGluArgAspGlnHisAsnArgAspLysAlaLeuLeuThr850855860tcacacttagcgcgcgccaaagatgctgtgagtgctgcacgccagcac2640SerHisLeuAlaArgAlaLysAspAlaValSerAlaAlaArgGlnHis865870875880ctcaatcgactcagcgacaacgcccactccatggaacttgcgcgcagc2688LeuAsnArgLeuSerAspAsnAlaHisSerMetGluLeuAlaArgSer885890895caagctcaagtgcgcatggaggaagccgttgccaaaatcaccgagcaa2736GlnAlaGlnValArgMetGluGluAlaValAlaLysIleThrGluGln900905910cttggcattccggtcgcagaattgctgcgcgattacaccccagatgaa2784LeuGlyIleProValAlaGluLeuLeuArgAspTyrThrProAspGlu915920925aactttgatgaaaagttccaacgggcacgcctcaaacaagccgaaaaa2832AsnPheAspGluLysPheGlnArgAlaArgLeuLysGlnAlaGluLys930935940gacctcgccgcactaggcaaagtcaaccccctggccttggaagaattc2880AspLeuAlaAlaLeuGlyLysValAsnProLeuAlaLeuGluGluPhe945950955960aaagccttggaagagcgctacgagttcctctccacccaattggccgat2928LysAlaLeuGluGluArgTyrGluPheLeuSerThrGlnLeuAlaAsp965970975gtggaacaagcccgcgccgatctgagcggcgtgattgaagaagtcgat2976ValGluGlnAlaArgAlaAspLeuSerGlyValIleGluGluValAsp980985990gcgaaaattctgcagcttttcaccgatgcctggaacgacgtggaagca3024AlaLysIleLeuGlnLeuPheThrAspAlaTrpAsnAspValGluAla99510001005gaattcccacgcgtgttcaacaccttgttcccaggtggcgaaggcaaa3072GluPheProArgValPheAsnThrLeuPheProGlyGlyGluGlyLys101010151020ctgatcctcaccgagcccgatgatttgctggcaaccggcatcgaagtg3120LeuIleLeuThrGluProAspAspLeuLeuAlaThrGlyIleGluVal1025103010351040gaagcccgcccaccaggaaaacgcgtcaagcgcctatcgctgctatcc3168GluAlaArgProProGlyLysArgValLysArgLeuSerLeuLeuSer104510501055ggtggcgaaaaatccctcaccgcactagccatgctcgtcgcgattttc3216GlyGlyGluLysSerLeuThrAlaLeuAlaMetLeuValAlaIlePhe106010651070cgcgcccgacccagccctttctacgtgatggatgaggtggaagcagca3264ArgAlaArgProSerProPheTyrValMetAspGluValGluAlaAla107510801085ctcgatgatgtcaaccttcgccgacttatcgcactgtttgaagagttg3312LeuAspAspValAsnLeuArgArgLeuIleAlaLeuPheGluGluLeu109010951100cgccgcgactcccagctcattgtgatcacccaccaaaaacccaccatg3360ArgArgAspSerGlnLeuIleValIleThrHisGlnLysProThrMet1105111011151120gatgtggctaacgtactctacggcgtcaccatgcgtggcgatggcgtt3408AspValAlaAsnValLeuTyrGlyValThrMetArgGlyAspGlyVal112511301135acccgcgtgatctcccaacgcatggaacctgcttctgcaatggaaact3456ThrArgValIleSerGlnArgMetGluProAlaSerAlaMetGluThr114011451150gtggcgggg3465ValAlaGly1155<210>38<211>1155<212>PRT<213>谷氨酸棒杆菌<400>38MetTyrLeuLysSerLeuThrLeuLysGlyPheLysSerPheAlaSer151015AlaThrThrLeuLysPheGluProGlyIleCysAlaValValGlyPro202530AsnGlySerGlyLysSerAsnValValAspAlaLeuAlaTrpValMet354045GlyGluGlySerAlaLysThrLeuArgGlyGlyLysMetGluAspVal505560IlePheAlaGlyAlaGlyAspArgLysProLeuGlyArgAlaGluVal65707580ThrLeuThrIleAspAsnSerAspGlyAlaLeuProIleGluTyrThr859095GluValSerValThrArgArgMetPheArgAspGlyAlaSerGluTyr100105110GluIleAsnGlyAlaLysAlaArgLeuMetAspIleGlnGluLeuLeu115120125SerAspThrGlyIleGlyArgGluMetHisIleMetValGlyGlnGly130135140LysLeuAlaGluIleLeuGluSerArgProGluGluArgArgAlaTyr145150155160IleGluGluAlaAlaGlyValLeuLysHisArgArgArgLysGluLys165170175AlaGlnArgLysLeuGlnGlyMetGlnValAsnLeuAspArgLeuGln180185190AspLeuThrHisGluLeuAlaLysGlnLeuLysProLeuAlaArgGln195200205AlaGluAlaAlaGlnArgAlaAlaThrValGlnAlaAspLeuArgAsp210215220AlaArgPheGlnIleAlaGlyPheGluIleValLysLeuSerGluLys225230235240LeuGluThrSerThrGluArgGluLysMetIleArgGluGlnAlaGlu245250255AlaAlaGlnGluGlnLeuGluGluAlaThrThrThrGlnMetGluVal260265270GluMetGluLeuAlaGluIleThrProGlnAlaGluAlaAlaGlnGln275280285LeuTrpPheAspLeuSerSerLeuAlaGluArgValSerAlaThrMet290295300ArgIleAlaAlaAspArgAlaSerSerGlyAlaAlaAspValProTyr305310315320AlaGlyGlnAspProAspGluLeuLeuGlyArgAlaGluThrAlaAsp325330335LysGluLeuGluGluLeuGluMetAlaValGluMetThrThrGluArg340345350LeuThrSerIleGlnGluGluAlaGluAspLysAlaAlaGlnAlaArg355360365GluAlaGluArgGluHisLeuAlaGlnValArgAlaIleSerAspArg370375380ArgGluGlyValValArgLeuLeuAlaSerGluGluSerLeuArgThr385390395400GlnHisThrSerAlaGluGluGluAlaGluArgLeuSerGluGlnLeu405410415GluGluPheIleGlyArgIleLeuAspValGluArgGluArgArgLeu420425430ThrAspGluArgLysGlnGlyValAspThrAspArgAlaProLeuGlu435440445GluAlaLeuLysGlnAlaLysHisGluAlaGluAlaAlaGluThrArg450455460LeuGluGluLeuArgThrLysArgSerAspLeuGluLysGluValSer4G5470475480ArgLeuGlnSerArgIleGluThrLeuAsnGlnAsnArgProArgSer485490495AspAlaAlaAspValValAspTyrProGlnLeuAlaThrLeuIleArg500505510ProGlnArgAsnValAspLysAlaLeuAlaAlaAlaLeuGlyAlaHis515520525AlaGluAlaLeuAlaGlyGluAlaAlaGluGlyLeuValGluLysLeu530535540IleAspAlaGlyValAlaArgThrIleIleValAspGlyThrGlnAla545550555560GlyGlyAlaTrpArgLeuAspAlaAsnIleProAlaGlyAlaSerTrp565570575LeuLeuAspHisValAspLeuAspProAlaIleAlaGlyProValAsn580585590ArgLeuLeuAlaAspValValLeuValAspAspProSerLeuGlyArg595600605GlnAlaIleGluAspAspProArgLeuArgAlaValAspArgAsnGly610615620ValLeuIleGlyAlaGlyTrpIleGlnValGlyThrGluThrSerThr625630635640ValGluIleThrAlaGlnIleGluGluAlaGluAlaGlnLeuAlaAla645650655AlaSerAlaAlaLeuAspAspIleAlaGlyThrPheAspGlyAlaLeu660665670HisAlaAlaAspAsnThrArgValGluValAlaAlaArgThrAlaAla675680685LeuArgGluLeuAspMetThrArgAspSerIleThrArgAspLeuAla690695700ArgLeuAspLysGlnHisGluAlaAlaGluSerGluArgValArgHis705710715720ValGlyArgLeuHisAlaAlaGluThrArgArgGluGluLeuArgGlu725730735GlnLeuGluAspIleValAspArgLeuSerArgValGluAspGluGlu740745750AspAlaAspGluProSerThrThrAlaArgAspGlnAlaAsnAlaGlu755760765LeuGlnGlnIleArgAlaMetGluMetGluAlaArgLeuAlaGlnArg770775780ThrAlaGluGluArgAlaGlyGlnGlnArgGlyLysGlyAspSerLeu785790795800ArgArgGlnAlaGluHisGluArgGlnAlaLysIleArgHisGluGln805810815AlaMetGluAlaArgArgArgArgThrGlnLeuAlaAlaAlaValGln820825830AsnGlyAlaArgAspValAlaGluArgValSerAlaAlaLeuAlaGln835840845AlaAlaIleGluArgAspGlnHisAsnArgAspLysAlaLeuLeuThr850855860SerHisLeuAlaArgAlaLysAspAlaValSerAlaAlaArgGlnHis865870875880LeuAsnArgLeuSerAspAsnAlaHisSerMetGluLeuAlaArgSer885890895GlnAlaGlnValArgMetGluGluAlaValAlaLysIleThrGluGln900905910LeuGlyIleProValAlaGluLeuLeuArgAspTyrThrProAspGlu915920925AsnPheAspGluLysPheGlnArgAlaArgLeuLysGlnAlaGluLys930935940AspLeuAlaAlaLeuGlyLysValAsnProLeuAlaLeuGluGluPhe945950955960LysAlaLeuGluGluArgTyrGluPheLeuSerThrGlnLeuAlaAsp965970975ValGluGlnAlaArgAlaAspLeuSerGlyValIleGluGluValAsp980985990AlaLysIleLeuGlnLeuPheThrAspAlaTrpAsnAspValGluAla99510001005GluPheProArgValPheAsnThrLeuPheProGlyGlyGluGlyLys101010151020LeuIleLeuThrGluProAspAspLeuLeuAlaThrGlyIleGluVal1025103010351040GluAlaArgProProGlyLysArgValLysArgLeuSerLeuLeuSer104510501055GlyGlyGluLysSerLeuThrAlaLeuAlaMetLeuValAlaIlePhe106010651070ArgAlaArgProSerProPheTyrValMetAspGluValGluAlaAla107510801085LeuAspAspValAsnLeuArgArgLeuIleAlaLeuPheGluGluLeu109010951100ArgArgAspSerGlnLeuIleValIleThrHisGlnLysProThrMet1105111011151120AspValAlaAsnValLeuTyrGlyValThrMetArgGlyAspGlyVal112511301135ThrArgValIleSerGlnArgMetGluProAlaSerAlaMetGluThr114011451150ValAlaGly1155<210>39<211>777<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(777)<223>RXA02016W<400>39atgtcaaactttgaaacgttcctctgggttgcctacccctggctgtgt48MetSerAsnPheGluThrPheLeuTrpValAlaTyrProTrpLeuCys151015atcgccgcctacatcatcggcatttcttggcgctggcgcgccgaccaa96IleAlaAlaTyrIleIleGlyIleSerTrpArgTrpArgAlaAspGln202530ttcggttggaccacccactcctcccaaatctacgaatccaaactcctc144PheGlyTrpThrThrHisSerSerGlnIleTyrGluSerLysLeuLeu354045cgcatcgcctccccactcttccactggggcatggtgttcgtggtgatc192ArgIleAlaSerProLeuPheHisTrpGlyMetValPheValValIle505560ggccacctcatgggacttgccatccccaagagctggacccaagctgta240GlyHisLeuMetGlyLeuAlaIleProLysSerTrpThrGlnAlaVal65707580ggaatttctgacgccgcttaccacctcatcgccaccatcccaggcacc288GlyIleSerAspAlaAlaTyrHisLeuIleAlaThrIleProGlyThr859095attgccggcatcgctgcagtccttggactcatcggcttgattatccgt336IleAlaGlyIleAlaAlaValLeuGlyLeuIleGlyLeuIleIleArg100105110cgcgtgatcaacaaaaccgtcttcctgtccacctcacgctccgacaaa384ArgValIleAsnLysThrValPheLeuSerThrSerArgSerAspLys115120125gtgatgtatgtgctactcggcgctgcaattttgtccggtttcatcgcc432ValMetTyrValLeuLeuGlyAlaAlaIleLeuSerGlyPheIleAla130135140accgtctccacccaggtcttcggcggcgcacacggctacgactaccgc480ThrValSerThrGlnValPheGlyGlyAlaHisGlyTyrAspTyrArg145150155160gaaaccatctccccatgggtgcgccaactgctcatcttcaacgctcaa528GluThrIleSerProTrpValArgGlnLeuLeuIlePheAsnAlaGln165170175ccagagctcatggctgatgtcccttgggaattcaaggtccacatcgtc576ProGluLeuMetAlaAspValProTrpGluPheLysValHisIleVal180185190gctggattcaccctcatcgcactgtggccattcacccgcctagtccac624AlaGlyPheThrLeuIleAlaLeuTrpProPheThrArgLeuValHis195200205gcgttctccgcaccagttggatacgtcacccgcccctacgtggtctat672AlaPheSerAlaProValGlyTyrValThrArgProTyrValValTyr210215220cgcacccgcgacaccacctctgaaccggcacgccaaaacgtcgcctgg720ArgThrArgAspThrThrSerGluProAlaArgGlnAsnValAlaTrp225230235240gaaccgatccgctcggtcaaaaatcagctcgacaatgactcgaaatgg768GluProIleArgSerValLysAsnGlnLeuAspAsnAspSerLysTrp245250255cacggcgcc777HisGlyAla<210>40<211>259<212>PRT<213>谷氨酸棒杆菌<400>40MetSerAsnPheGluThrPheLeuTrpValAlaTyrProTrpLeuCys151015IleAlaAlaTyrIleIleGlyIleSerTrpArgTrpArgAlaAspGln202530PheGlyTrpThrThrHisSerSerGlnIleTyrGluSerLysLeuLeu354045ArgIleAlaSerProLeuPheHisTrpGlyMetValPheValValIle505560GlyHisLeuMetGlyLeuAlaIleProLysSerTrpThrGlnAlaVal65707580GlyIleSerAspAlaAlaTyrHisLeuIleAlaThrIleProGlyThr859095IleAlaGlyIleAlaAlaValLeuGlyLeuIleGlyLeuIleIleArg100105110ArgValIleAsnLysThrValPheLeuSerThrSerArgSerAspLys115120125ValMetTyrValLeuLeuGlyAlaAlaIleLeuSerGlyPheIleAla130135140ThrValSerThrGlnValPheGlyGlyAlaHisGlyTyrAspTyrArg145150155160GluThrIleSerProTrpValArgGlnLeuLeuIlePheAsnAlaGln165170175ProGluLeuMetAlaAspValProTrpGluPheLysValHisIleVal180185190AlaGlyPheThrLeuIleAlaLeuTrpProPheThrArgLeuValHis195200205AlaPheSerAlaProValGlyTyrValThrArgProTyrValValTyr210215220ArgThrArgAspThrThrSerGluProAlaArgGlnAsnValAlaTrp225230235240GluProIleArgSerValLysAsnGlnLeuAspAsnAspSerLysTrp245250255HisGlyAla<210>41<211>969<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(969)<223>RXA02149W<400>41atgccacaaaaaccggccagtttcgcggtgggctttgacatcggcggc48MetProGlnLysProAlaSerPheAlaValGlyPheAspIleGlyGly151015accaacatgcgagccgggcttgtcgacgaatccgggcgcatcgtgacc96ThrAsnMetArgAlaGlyLeuValAspGluSerGlyArgIleValThr202530agtttgtcggcgccgtcgccgcgcacgacgcaggcaatggaacagggg144SerLeuSerAlaProSerProArgThrThrGlnAlaMetGluGlnGly354045atttttgatctagtcgaacagctcaaggccgaatacccggttggtgct192IlePheAspLeuValGluGlnLeuLysAlaGluTyrProValGlyAla505560gtgggacttgccgtcgcgggatttttggatcctgagtgcgaggttgtt240ValGlyLeuAlaValAlaGlyPheLeuAspProGluCysGluValVal65707580cgatttgccccgcaccttccttggcgcgatgagccagtgcgtgaaaag288ArgPheAlaProHisLeuProTrpArgAspGluProValArgGluLys859095ttggaaaaccttttgggcctgcctgttcgtttggaacatgatgccaac336LeuGluAsnLeuLeuGlyLeuProValArgLeuGluHisAspAlaAsn100105110tcagcagcgtggggtgagcatcgttttggtgcagctcaaggcgctgac384SerAlaAlaTrpGlyGluHisArgPheGlyAlaAlaGlnGlyAlaAsp115120125aactgggttttgttggcactcggcactggaattggtgcagcgctgatt432AsnTrpValLeuLeuAlaLeuGlyThrGlyIleGlyAlaAlaLeuIle130135140gaaaaaggcgaaatttaccgtggtgcatatggcacggcaccagaattt480GluLysGlyGluIleTyrArgGlyAlaTyrGlyThrAlaProGluPhe145150155160ggtcatttgcgtgttgttcgtggcggacgcgcatgtgcgtgtggcaaa528GlyHisLeuArgValValArgGlyGlyArgAlaCysAlaCysGlyLys165170175gaaggctgcctggagcgttactgttccggtactgccttggtttacact576GluGlyCysLeuGluArgTyrCysSerGlyThrAlaLeuValTyrThr180185190gcgcgtgaattggcttcgcatggctcattccgcaacagcgggctgttt624AlaArgGluLeuAlaSerHisGlySerPheArgAsnSerGlyLeuPhe195200205gacaagatcaaagccgatccgaattccatcaatggaaaaacgatcact672AspLysIleLysAlaAspProAsnSerIleAsnGlyLysThrIleThr210215220gcggcagcgcgccaagaagacccacttgctctcgccgttctggaagat720AlaAlaAlaArgGlnGluAspProLeuAlaLeuAlaValLeuGluAsp225230235240ttcagcgagtggctgggcgaaactttggcgatcattgctgatgtcctt768PheSerGluTrpLeuGlyGluThrLeuAlaIleIleAlaAspValLeu245250255gacccaggcatgatcatcattggtggcggactgtccaatgctgccgac816AspProGlyMetIleIleIleGlyGlyGlyLeuSerAsnAlaAlaAsp260265270ctttatttggatcgctcggtcaaccactattccacccgcatcgtcggc864LeuTyrLeuAspArgSerValAsnHisTyrSerThrArgIleValGly275280285gcaggatatcgccctttggcacgcgttgccacagctcagttgggtgcg912AlaGlyTyrArgProLeuAlaArgValAlaThrAlaGlnLeuGlyAla290295300gatgctggcatgatcggtgtcgctgatctagctcgacgctctgtagtg960AspAlaGlyMetIleGlyValAlaAspLeuAlaArgArgSerValVal305310315320gaagccaac969GluAlaAsn<210>42<211>323<212>PRT<213>谷氨酸棒杆菌<400>42MetProGlnLysProAlaSerPheAlaValGlyPheAspIleGlyGly151015ThrAsnMetArgAlaGlyLeuValAspGluSerGlyArgIleValThr202530SerLeuSerAlaProSerProArgThrThrGlnAlaMetGluGlnGly354045IlePheAspLeuValGluGlnLeuLysAlaGluTyrProValGlyAla505560ValGlyLeuAlaValAlaGlyPheLeuAspProGluCysGluValVal65707580ArgPheAlaProHisLeuProTrpArgAspGluProValArgGluLys859095LeuGluAsnLeuLeuGlyLeuProValArgLeuGluHisAspAlaAsn100105110SerAlaAlaTrpGlyGluHisArgPheGlyAlaAlaGlnGlyAlaAsp115120125AsnTrpValLeuLeuAlaLeuGlyThrGlyIleGlyAlaAlaLeuIle130135140GluLysGlyGluIleTyrArgGlyAlaTyrGlyThrAlaProGluPhe145150155160GlyHisLeuArgValValArgGlyGlyArgAlaCysAlaCysGlyLys165170175GluGlyCysLeuGluArgTyrCysSerGlyThrAlaLeuValTyrThr180185190AlaArgGluLeuAlaSerHisGlySerPheArgAsnSerGlyLeuPhe195200205AspLysIleLysAlaAspProAsnSerIleAsnGlyLysThrIleThr210215220AlaAlaAlaArgGlnGluAspProLeuAlaLeuAlaValLeuGluAsp225230235240PheSerGluTrpLeuGlyGluThrLeuAlaIleIleAlaAspValLeu245250255AspProGlyMetIleIleIleGlyGlyGlyLeuSerAsnAlaAlaAsp260265270LeuTyrLeuAspArgSerValAsnHisTyrSerThrArgIleValGly275280285AlaGlyTyrArgProLeuAlaArgValAlaThrAlaGlnLeuGlyAla290295300AspAlaGlyMetIleGlyValAlaAspLeuAlaArgArgSerValVal305310315320GluAlaAsn<210>43<211>1068<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1068)<223>RXA02206E<400>43atggtttgtacccagccaatattttcagttgcggcaggatctgctgct48MetValCysThrGlnProIlePheSerValAlaAlaGlySerAlaAla151015gcggctaggagggttatctcttcattcacccgatctaccgtactacct96AlaAlaArgArgValIleSerSerPheThrArgSerThrValLeuPro202530tatgacctcagtagtgtggtgggcgtgaaacagcgaatggtcggttca144TyrAspLeuSerSerValValGlyValLysGlnArgMetValGlySer354045agtggtttgcgggtatccaggctcggtttgggcacctcaacatggggc192SerGlyLeuArgValSerArgLeuGlyLeuGlyThrSerThrTrpGly505560tcgggcaccgagctggctgaggcaggcgatatctttaaggcgttcatc240SerGlyThrGluLeuAlaGluAlaGlyAspIlePheLysAlaPheIle65707580aattctggtggcacgcttatcgacgtctcccccaactacaccaccggc288AsnSerGlyGlyThrLeuIleAspValSerProAsnTyrThrThrGly859095gtcgcggaagaaatgctcggcacgatgttggatgcggaagtctctcgt336ValAlaGluGluMetLeuGlyThrMetLeuAspAlaGluValSerArg100105110tcggctgtcgtcatttcctccagcgcaggtgtcaaccccgctctgccg384SerAlaValValIleSerSerSerAlaGlyValAsnProAlaLeuPro115120125ctcggccgacgtgtggattgctcccgccgcaatttgattgcccaatta432LeuGlyArgArgValAspCysSerArgArgAsnLeuIleAlaGlnLeu130135140gatgtcaccctgcgggcattaaacactgactatttggatttgtggtct480AspValThrLeuArgAlaLeuAsnThrAspTyrLeuAspLeuTrpSer145150155160gtgggctattgggatgagggcaccccaccgcatgaggtggccgatact528ValGlyTyrTrpAspGluGlyThrProProHisGluValAlaAspThr165170175ttggattacgccgtgcgcaccggccgagtccgatatgccggtgtccga576LeuAspTyrAlaValArgThrGlyArgValArgTyrAlaGlyValArg180185190ggatattccggttggcagttagcggtcacccacgctgcatccaatcat624GlyTyrSerGlyTrpGlnLeuAlaValThrHisAlaAlaSerAsnHis195200205gcagcggcctccgcccgccccgtggtcgttgcacaaaatgaatacagc672AlaAlaAlaSerAlaArgProValValValAlaGlnAsnGluTyrSer210215220ctgctggaacgccgcgcagaacaagaactcctccctgccacccaacac720LeuLeuGluArgArgAlaGluGlnGluLeuLeuProAlaThrGlnHis225230235240ctaggtgtcggattctttgctggcgctccgctggggcaaggcgtgctg768LeuGlyValGlyPhePheAlaGlyAlaProLeuGlyGlnGlyValLeu245250255actgctaaataccgctccgaaattccccatgattccagagctgcatcc816ThrAlaLysTyrArgSerGluIleProHisAspSerArgAlaAlaSer260265270acaggacgcgacgcagaagtccaaagctacctagataatcgaggccgc864ThrGlyArgAspAlaGluValGlnSerTyrLeuAspAsnArgGlyArg275280285atcattgtcgatgctcttgatactgcagccaaaggattaggcattagc912IleIleValAspAlaLeuAspThrAlaAlaLysGlyLeuGlyIleSer290295300cccgctgtcacagccaccacctgggtgcgtgatcgtcccggagtgaca960ProAlaValThrAlaThrThrTrpValArgAspArgProGlyValThr305310315320gctgtcatcgtgggcgctcgcacacatgaacagctgtcacatcttctc1008AlaVallleValGlyAlaArgThrHisGluGlnLeuSerHisLeuLeu325330335aaggcggaatcggtgactttgccaacaccaatcacacaagcccttgat1056LysAlaGluSerValThrLeuProThrProIleThrGlnAlaLeuAsp340345350gatgtctccctg1068AspValSerLeu355<210>44<211>356<212>PRT<213>谷氨酸棒杆菌<400>44MetValCysThrGlnProIlePheSerValAlaAlaGlySerAlaAla151015AlaAlaArgArgValIleSerSerPheThrArgSerThrValLeuPro202530TyrAspLeuSerSerValValGlyValLysGlnArgMetValGlySer354045SerGlyLeuArgValSerArgLeuGlyLeuGlyThrSerThrTrpGly505560SerGlyThrGluLeuAlaGluAlaGlyAspIlePheLysAlaPheIle65707580AsnSerGlyGlyThrLeuIleAspValSerProAsnTyrThrThrGly859095ValAlaGluGluMetLeuGlyThrMetLeuAspAlaGluValSerArg100105110SerAlaValValIleSerSerSerAlaGlyValAsnProAlaLeuPro115120125LeuGlyArgArgValAspCysSerArgArgAsnLeuIleAlaGlnLeu130135140AspValThrLeuArgAlaLeuAsnThrAspTyrLeuAspLeuTrpSer145150155160ValGlyTyrTrpAspGluGlyThrProProHisGluValAlaAspThr165170175LeuAspTyrAlaValArgThrGlyArgValArgTyrAlaGlyValArg180185190GlyTyrSerGlyTrpGlnLeuAlaValThrHisAlaAlaSerAsnHis195200205AlaAlaAlaSerAlaArgProValValValAlaGlnAsnGluTyrSer210215220LeuLeuGluArgArgAlaGluGlnGluLeuLeuProAlaThrGlnHis225230235240LeuGlyValGlyPhePheAlaGlyAlaProLeuGlyGlnGlyValLeu245250255ThrAlaLysTyrArgSerGluIleProHisAspSerArgAlaAlaSer260265270ThrGlyArgAspAlaGluValGlnSerTyrLeuAspAsnArgGlyArg275280285IleIleValAspAlaLeuAspThrAlaAlaLysGlyLeuGlyIleSer290295300ProAlaValThrAlaThrThrTrpValArgAspArgProGlyValThr305310315320AlaValIleValGlyAlaArgThrHisGluGlnLeuSerHisLeuLeu325330335LysAlaGluSerValThrLeuProThrProIleThrGlnAlaLeuAsp340345350AspValSerLeu355<2l0>45<211>948<212>DNA<2l3>谷氨酸棒杆菌<220><22l>CDS<222>(1)..(948)<223>RXA02206K<400>45gtgaaacagcgaatggtcggttcaagtggtttgcgggtatccaggctc48MetLysGlnArgMetValGlySerSerGlyLeuArgValSerArgLeu151015ggtttgggcacctcaacatggggctcgggcaccgagctggctgaggca96GlyLeuGlyThrSerThrTrpGlySerGlyThrGluLeuAlaGluAla202530ggcgatatctttaaggcgttcatcaattctggtggcacgcttatcgac144GlyAspIlePheLysAlaPheIleAsnSerGlyGlyThrLeuIleAsp354045gtctcccccaactacaccaccggcgtcgcggaagaaatgctcggcacg192ValSerProAsnTyrThrThrGlyValAlaGluGluMetLeuGlyThr505560atgttggatgcggaagtctctcgttcggctgtcgtcatttcctccagc240MetLeuAspAlaGluValSerArgSerAlaValValIleSerSerSer65707580gcaggtgtcaaccccgctctgccgctcggccgacgtgtggattgctcc288AlaGlyValAsnProAlaLeuProLeuGlyArgArgValAspCysSer859095cgccgcaatttgattgcccaattagatgtcaccctgcgggcattaaac336ArgArgAsnLeuIleAlaGlnLeuAspValThrLeuArgAlaLeuAsn100105110actgactatttggatttgtggtctgtgggctattgggatgagggcacc384ThrAspTyrLeuAspLeuTrpSerValGlyTyrTrpAspGluGlyThr115120125ccaccgcatgaggtggccgatactttggattacgccgtgcgcaccggc432ProProHisGluValAlaAspThrLeuAspTyrAlaValArgThrGly130135140cgagtccgatatgccggtgtccgaggatattccggttggcagttagcg480ArgValArgTyrAlaGlyValArgGlyTyrSerGlyTrpGlnLeuAla145150155160gtcacccacgctgcatccaatcatgcagcggcctccgcccgccccgtg528ValThrHisAlaAlaSerAsnHisAlaAlaAlaSerAlaArgProVal165170175gtcgttgcacaaaatgaatacagcctgctggaacgccgcgcagaacaa576ValValAlaGlnAsnGluTyrSerLeuLeuGluArgArgAlaGluGln180185190gaactcctccctgccacccaacacctaggtgtcggattctttgctggc624GluLeuLeuProAlaThrGlnHisLeuGlyValGlyPhePheAlaGly195200205gctccgctggggcaaggcgtgctgactgctaaataccgctccgaaatt672AlaProLeuGlyGlnGlyValLeuThrAlaLysTyrArgSerGluIle210215220ccccatgattccagagctgcatccacaggacgcgacgcagaagtccaa720ProHisAspSerArgAlaAlaSerThrGlyArgAspAlaGluValGln225230235240agctacctagataatcgaggccgcatcattgtcgatgctcttgatact768SerTyrLeuAspAsnArgGlyArgIleIleValAspAlaLeuAspThr245250255gcagccaaaggattaggcattagccccgctgtcacagccaccacctgg816AlaAlaLysGlyLeuGlyIleSerProAlaValThrAlaThrThrTrp260265270gtgcgtgatcgtcccggagtgacagctgtcatcgtgggcgctcgcaca864ValArgAspArgProGlyValThrAlaValIleValGlyAlaArgThr275280285catgaacagctgtcacatcttctcaaggcggaatcggtgactttgcca912HisGluGlnLeuSerHisLeuLeuLysAlaGluSerValThrLeuPro290295300acaccaatcacacaagcccttgatgatgtctccctg948ThrProIleThrGlnAlaLeuAspAspValSerLeu305310315<210>46<211>316<212>PRT<213>谷氨酸棒杆菌<400>46MetLysGlnArgMetValGlySerSerGlyLeuArgValSerArgLeu151015GlyLeuGlyThrSerThrTrpGlySerGlyThrGluLeuAlaGluAla202530GlyAspIlePheLysAlaPheIleAsnSerGlyGlyThrLeuIleAsp354045ValSerProAsnTyrThrThrGlyValAlaGluGluMetLeuGlyThr505560MetLeuAspAlaGluValSerArgSerAlaValValIleSerSerSer65707580AlaGlyValAsnProAlaLeuProLeuGlyArgArgValAspCysSer859095ArgArgAsnLeuIleAlaGlnLeuAspValThrLeuArgAlaLeuAsn100105110ThrAspTyrLeuAspLeuTrpSerValGlyTyrTrpAspGluGlyThr115120125ProProHisGluValAlaAspThrLeuAspTyrAlaValArgThrGly130135140ArgValArgTyrAlaGlyValArgGlyTyrSerGlyTrpGlnLeuAla145150155160ValThrHisAlaAlaSerAsnHisAlaAlaAlaSerAlaArgProVal165170175ValValAlaGlnAsnGluTyrSerLeuLeuGluArgArgAlaGluGln180185190GluLeuLeuProAlaThrGlnHisLeuGlyValGlyPhePheAlaGly195200205AlaProLeuGlyGlnGlyValLeuThrAlaLysTyrArgSerGluIle210215220ProHisAspSerArgAlaAlaSerThrGlyArgAspAlaGluValGln225230235240SerTyrLeuAspAsnArgGlyArgIleIleValAspAlaLeuAspThr245250255AlaAlaLysGlyLeuGlyIleSerProAlaValThrAlaThrThrTrp260265270ValArgAspArgProGlyValThrAlaVallleValGlyAlaArgThr275280285HisGluGlnLeuSerHisLeuLeuLysAlaGluSerValThrLeuPro290295300ThrProIleThrGlnAlaLeuAspAspValSerLeu305310315<210>47<211>408<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(408)<223>RXA02299W<400>47atgctgcgcaccatcctcggaagtaagattcaccgagccactgtcact48MetLeuArgThrIleLeuGlySerLysIleHisArgAlaThrValThr151015caagctgatctagattatgttggctctgtaaccatcgacgccgacctg96GlnAlaAspLeuAspTyrValGlySerValThrIleAspAlaAspLeu202530gttcacgccgccggattgatcgaaggcgaaaaagttgccatcgtagac144ValHisAlaAlaGlyLeuIleGluGlyGluLysValAlaIleValAsp354045atcaccaacggcgctcgtctggaaacttatgtcattgtgggcgacgcc192IleThrAsnGlyAlaArgLeuGluThrTyrValIleValGlyAspAla505560ggaacgggcaatatttgcatcaatggtgccgctgcacaccttattaat240GlyThrGlyAsnIleCysIleAsnGlyAlaAlaAlaHisLeuIleAsn65707580cctggcgatcttgtgatcatcatgagctaccttcaggcaactgatgcg288ProGlyAspLeuValIleIleMetSerTyrLeuGlnAlaThrAspAla859095gaagccaaggcgtatgagccaaagattgtgcacgtggacgccgacaac336GluAlaLysAlaTyrGluProLysIleValHisValAspAlaAspAsn100105110cgcatcgttgcgctcggcaacgatcttgcggaagcactacctggatcc384ArgIleValAlaLeuGlyAsnAspLeuAlaGluAlaLeuProGlySer115120125gggcttttgacgtcgagaagcatt408GlyLeuLeuThrSerArgSerIle130135<210>48<211>136<212>PRT<213>谷氨酸棒杆菌<400>48MetLeuArgThrIleLeuGlySerLysIleHisArgAlaThrValThr151015GlnAlaAspLeuAspTyrValGlySerValThrIleAspAlaAspLeu202530ValHisAlaAlaGlyLeuIleGluGlyGluLysValAlaIleValAsp354045IleThrAsnGlyAlaArgLeuGluThrTyrValIleValGlyAspAla505560GlyThrGlyAsnIleCysIleAsnGlyAlaAlaAlaHisLeuIleAsn65707580ProGlyAspLeuValIleIleMetSerTyrLeuGlnAlaThrAspAla859095GluAlaLysAlaTyrGluProLysIleValHisValAspAlaAspAsn100105110ArgIleValAlaLeuGlyAsnAspLeuAlaGluAlaLeuProGlySer115120125GlyLeuLeuThrSerArgSerIle130135<210>49<211>669<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(669)<223>RXA02390W<400>49gtggagtggaccgcttttggcaccctgattctgctcaatttggtgggc48MetGluTrpThrAlaPheGlyThrLeuIleLeuLeuAsnLeuValGly151015agtttatccccggggcctgatacctttttcctcctccgcttagccacc96SerLeuSerProGlyProAspThrPhePheLeuLeuArgLeuAlaThr202530cgctccagagcgcacgcgatcgctggcgtcgccggcatcgtcaccgga144ArgSerArgAlaHisAlaIleAlaGlyValAlaGlyIleValThrGly354045ctcacggtgtgggtgacgctgacggtcgtgggagcagcggcgctgctc192LeuThrValTrpValThrLeuThrValValGlyAlaAlaAlaLeuLeu505560accacttatccgtcgattctcggaatcatccagctcgtcggcggcacg240ThrThrTyrProSerIleLeuGlyIleIleGlnLeuValGlyGlyThr65707580tacctaagcttcattgggtacaagttgctgcgctcggcgtcgagagag288TyrLeuSerPheIleGlyTyrLysLeuLeuArgSerAlaSerArgGlu859095cttatcgacgcccgccagttccgtttcaacgccgatgcccgacctatc336LeuIleAspAlaArgGlnPheArgPheAsnAlaAspAlaArgProIle100105110ccggatgcggtagaagcactgggaacccgcactcaggtatatcgacaa384ProAspAlaValGluAlaLeuGlyThrArgThrGlnValTyrArgGln115120125ggtttggccaccaacctgtcaaaccctaaagttgtcatgtacttcgcg432GlyLeuAlaThrAsnLeuSerAsnProLysValValMetTyrPheAla130135140gcaattctggctccgttgatgccagcgcacccatcaccggtgctggcg480AlaIleLeuAlaProLeuMetProAlaHisProSerProValLeuAla145150155160ttctctatcatcgtggcgattttagtgcagacctttgttaccttctct528PheSerIleIleValAlaIleLeuValGlnThrPheValThrPheSer165170175gctgtgtgcctcattgtctctacggagcgtgtgcgcaaagcaatgctg576AlaValCysLeuIleValSerThrGluArgValArgLysAlaMetLeu180185190cgtgcaggtccctggtttgacctgcttgctggcgttgtcttcctcgtt624ArgAlaGlyProTrpPheAspLeuLeuAlaGlyValValPheLeuVal195200205gtgggtgtgactctgctgtatgaaggcctgaccggtttactcggg669ValGlyValThrLeuLeuTyrGluGlyLeuThrGlyLeuLeuGly210215220<210>50<211>223<212>PRT<213>谷氨酸棒杆菌<400>50MetGluTrpThrAlaPheGlyThrLeuIleLeuLeuAsnLeuValGly151015SerLeuSerProGlyProAspThrPhePheLeuLeuArgLeuAlaThr202530ArgSerArgAlaHisAlaIleAlaGlyValAlaGlyIleValThrGly354045LeuThrValTrpValThrLeuThrValValGlyAlaAlaAlaLeuLeu505560ThrThrTyrProSerIleLeuGlyIleIleGlnLeuValGlyGlyThr65707580TyrLeuSerPheIleGlyTyrLysLeuLeuArgSerAlaSerArgGlu859095LeuIleAspAlaArgGlnPheArgPheAsnAlaAspAlaArgProIle100105110ProAspAlaValGluAlaLeuGlyThrArgThrGlnValTyrArgGln115120125GlyLeuAlaThrAsnLeuSerAsnProLysValValMetTyrPheAla130135140AlaIleLeuAlaProLeuMetProAlaHisProSerProValLeuAla145150155160PheSerIleIleValAlaIleLeuValGlnThrPheValThrPheSer165170175AlaValCysLeuIleValSerThrGluArgValArgLysAlaMetLeu180185190ArgAlaGlyProTrpPheAspLeuLeuAlaGlyValValPheLeuVal195200205ValGlyValThrLeuLeuTyrGluGlyLeuThrGlyLeuLeuGly210215220<210>51<211>1344<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1344)<223>RXA02399E<400>51gtggtcggcaccgcccattgcgaatcagcacttaaggaagtgactttg48MetValGlyThrAlaHisCysGluSerAlaLeuLysGluValThrLeu151015atgtcaaacgttggaaagccacgtaccgcacaggaaatccagcaggat96MetSerAsnValGlyLysProArgThrAlaGlnGluIleGlnGlnAsp202530tgggacaccaaccctcgttggaacggcatcacccgcgactacaccgca144TrpAspThrAsnProArgTrpAsnGlyIleThrArgAspTyrThrAla354045gaccaggtagctgatctgcagggttccgtcatcgaggagcacactctt192AspGlnValAlaAspLeuGlnGlySerValIleGluGluHisThrLeu505560gctcgccgcggctcagagatcctctgggacgcagtcacccaggaaggt240AlaArgArgGlySerGluIleLeuTrpAspAlaValThrGlnGluGly65707580gacggatacatcaacgcgcttggcgcactcaccggtaaccaggctgtt288AspGlyTyrIleAsnAlaLeuGlyAlaLeuThrGlyAsnGlnAlaVal859095cagcaggttcgtgcaggcctgaaggctgtctacctgtccggttggcag336GlnGlnValArgAlaGlyLeuLysAlaValTyrLeuSerGlyTrpGln100105110gtcgcaggtgacgccaacctctccggccacacctaccctgaccagtcc384ValAlaGlyAspAlaAsnLeuSerGlyHisThrTyrProAspGlnSer115120125ctctacccagcgaactccgttccaagcgtcgttcgtcgcatcaacaac432LeuTyrProAlaAsnSerValProSerValValArgArgIleAsnAsn130135140gcactgctgcgttccgatgaaatcgcacgcaccgaaggcgacacctcc480AlaLeuLeuArgSerAspGluIleAlaArgThrGluGlyAspThrSer145150155160gttgacaactgggttgtcccaatcgtcgcggacggcgaagctggcttc528ValAspAsnTrpValValProIleValAlaAspGlyGluAlaGlyPhe165170175ggtggagcactcaacgtctacgaactccagaaggcaatgatcgcagct576GlyGlyAlaLeuAsnValTyrGluLeuGlnLysAlaMetIleAlaAla180185190ggcgctgcaggcacccactgggaagaccagctcgcttctgaaaagaag624GlyAlaAlaGlyThrHisTrpGluAspGlnLeuAlaSerGluLysLys195200205tgtggccacctcggcggcaaggttctgatcccaacccagcagcacatc672CysGlyHisLeuGlyGlyLysValLeuIleProThrGlnGlnHisIle210215220cgcaccctgaactctgcccgccttgcagcagacgttgcaaacacccca720ArgThrLeuAsnSerAlaArgLeuAlaAlaAspValAlaAsnThrPro225230235240actgttgttatcgcacgtaccgacgctgaggcagcaaccctgatcacc768ThrValValIleAlaArgThrAspAlaGluAlaAlaThrLeuIleThr245250255tctgacgttgatgagcgcgaccaaccattcatcaccggtgagcgcacc816SerAspValAspGluArgAspGlnProPheIleThrGlyGluArgThr260265270gcagaaggctactaccacgtcaagaatggtctcgagccatgtatcgca864AlaGluGlyTyrTyrHisValLysAsnGlyLeuGluProCysIleAla275280285cgtgcaaagtcctacgcaccatacgcagatatgatctggatggagacc912ArgAlaLysSerTyrAlaProTyrAlaAspMetIleTrpMetGluThr290295300ggcacccctgacctggagctcgctaagaagttcgctgaaggcgttcgc960GlyThrProAspLeuGluLeuAlaLysLysPheAlaGluGlyValArg305310315320tctgagttcccagaccagctgctgtcctacaactgctccccatccttc1008SerGluPheProAspGlnLeuLeuSerTyrAsnCysSerProSerPhe325330335aactggtctgcacacctcgaggcagatgagatcgctaagttccagaag1056AsnTrpSerAlaHisLeuGluAlaAspGluIleAlaLysPheGlnLys340345350gaactcggcgcaatgggcttcaagttccagttcatcaccctcgcaggc1104GluLeuGlyAlaMetGlyPheLysPheGlnPheIleThrLeuAlaGly355360365ttccactccctcaactacggcatgttcgacctggcttacggatacgct1152PheHisSerLeuAsnTyrGlyMetPheAspLeuAlaTyrGlyTyrAla370375380cgcgaaggcatgacctccttcgttgacctgcagaaccgtgagttcaag1200ArgGluGlyMetThrSerPheValAspLeuGlnAshArgGluPheLys385390395400gcagctgaagagcgtggcttcaccgctgttaagcaccagcgtgaggtt1248AlaAlaGluGluArgGlyPheThrAlaValLysHisGlnArgGluVal405410415ggcgcaggctacttcgaccagatcgcaaccaccgttgacccgaactct1296GlyAlaGlyTyrPheAspGlnIleAlaThrThrValAspProAsnSer420425430tctaccaccgctttgaagggttccactgaagaaggccagttccacaac1344SerThrThrAlaLeuLysGlySerThrGluGluGlyGlnPheHisAsn435440445<210>52<211>448<212>PRT<213>谷氨酸棒杆菌<400>52MetValGlyThrAlaHisCysGluSerAlaLeuLysGluValThrLeu151015MetSerAsnValGlyLysProArgThrAlaGlnGluIleGlnGlnAsp202530TrpAspThrAsnProArgTrpAsnGlyIleThrArgAspTyrThrAla354045AspGlnValAlaAspLeuGlnGlySerValIleGluGluHisThrLeu505560AlaArgArgGlySerGluIleLeuTrpAspAlaValThrGlnGluGly65707580AspGlyTyrIleAsnAlaLeuGlyAlaLeuThrGlyAsnGlnAlaVal859095GlnGlnValArgAlaGlyLeuLysAlaValTyrLeuSerGlyTrpGln100105110ValAlaGlyAspAlaAsnLeuSerGlyHisThrTyrProAspGlnSer115120125LeuTyrProAlaAsnSerValProSerValValArgArgIleAsnAsn130135140AlaLeuLeuArgSerAspGluIleAlaArgThrGluGlyAspThrSer145150155160ValAspAsnTrpValValProIleValAlaAspGlyGluAlaGlyPhe165170175GlyGlyAlaLeuAsnValTyrGluLeuGlnLysAlaMetIleAlaAla180185190GlyAlaAlaGlyThrHisTrpGluAspGlnLeuAlaSerGluLysLys195200205CysGlyHisLeuGlyGlyLysValLeuIleProThrGlnGlnHisIle210215220ArgThrLeuAsnSerAlaArgLeuAlaAlaAspValAlaAsnThrPro225230235240ThrValValIleAlaArgThrAspAlaGluAlaAlaThrLeuIleThr245250255SerAspValAspGluArgAspGlnProPheIleThrGlyGluArgThr260265270AlaGluGlyTyrTyrHisValLysAsnGlyLeuGluProCysIleAla275280285ArgAlaLysSerTyrAlaProTyrAlaAspMetIleTrpMetGluThr290295300GlyThrProAspLeuGluLeuAlaLysLysPheAlaGluGlyValArg305310315320SerGluPheProAspGlnLeuLeuSerTyrAsnCysSerProSerPhe325330335AsnTrpSerAlaHisLeuGluAlaAspGluIleAlaLysPheGlnLys340345350GluLeuGlyAlaMetGlyPheLysPheGlnPheIleThrLeuAlaGly355360365PheHisSerLeuAsnTyrGlyMetPheAspLeuAlaTyrGlyTyrAla370375380ArgGluGlyMetThrSerPheValAspLeuGlnAsnArgGluPheLys385390395400AlaAlaGluGluArgGlyPheThrAlaValLysHisGlnArgGluVal405410415GlyAlaGlyTyrPheAspGlnIleAlaThrThrValAspProAsnSer420425430SerThrThrAlaLeuLysGlySerThrGluGluGlyGlnPheHisAsn435440445<210>53<211>1296<212>DNA<213>谷氨酸棒杆菌<220><22l>CDS<222>(1)..(1296)<223>RXA02399W<400>53atgtcaaacgttggaaagccacgtaccgcacaggaaatccagcaggat48MetSerAsnValGlyLysProArgThrAlaGlnGluIleGlnGlnAsp151015tgggacaccaaccctcgttggaacggcatcacccgcgactacaccgca96TrpAspThrAsnProArgTrpAsnGlyIleThrArgAspTyrThrAla202530gaccaggtagctgatctgcagggttccgtcatcgaggagcacactctt144AspGlnValAlaAspLeuGlnGlySerValIleGluGluHisThrLeu354045gctcgccgcggctcagagatcctctgggacgcagtcacccaggaaggt192AlaArgArgGlySerGluIleLeuTrpAspAlaValThrGlnGluGly505560gacggatacatcaacgcgcttggcgcactcaccggtaaccaggctgtt240AspGlyTyrIleAsnAlaLeuGlyAlaLeuThrGlyAsnGlnAlaVal65707580cagcaggttcgtgcaggcctgaaggctgtctacctgtccggttggcag288GlnGlnValArgAlaGlyLeuLysAlaValTyrLeuSerGlyTrpGln859095gtcgcaggtgacgccaacctctccggccacacctaccctgaccagtcc336ValAlaGlyAspAlaAsnLeuSerGlyHisThrTyrProAspGlnSer100105110ctctacccagcgaactccgttccaagcgtcgttcgtcgcatcaacaac384LeuTyrProAlaAsnSerValProSerValValArgArgIleAsnAsn115120125gcactgctgcgttccgatgaaatcgcacgcaccgaaggcgacacctcc432AlaLeuLeuArgSerAspGluIleAlaArgThrGluGlyAspThrSer130135140gttgacaactgggttgtcccaatcgtcgcggacggcgaagctggcttc480ValAspAsnTrpValValProIleValAlaAspGlyGluAlaGlyPhe145150155160ggtggagcactcaacgtctacgaactccagaaggcaatgatcgcagct528GlyGlyAlaLeuAsnValTyrGluLeuGlnLysAlaMetIleAlaAla165170175ggcgctgcaggcacccactgggaagaccagctcgcttctgaaaagaag576GlyAlaAlaGlyThrHisTrpGluAspGlnLeuAlaSerGluLysLys180185190tgtggccacctcggcggcaaggttctgatcccaacccagcagcacatc624CysGlyHisLeuGlyGlyLysValLeuIleProThrGlnGlnHisIle195200205cgcaccctgaactctgcccgccttgcagcagacgttgcaaacacccca672ArgThrLeuAsnSerAlaArgLeuAlaAlaAspValAlaAsnThrPro210215220actgttgttatcgcacgtaccgacgctgaggcagcaaccctgatcacc720ThrValValIleAlaArgThrAspAlaGluAlaAlaThrLeuIleThr225230235240tctgacgttgatgagcgcgaccaaccattcatcaccggtgagcgcacc768SerAspValAspGluArgAspGlnProPheIleThrGlyGluArgThr245250255gcagaaggctactaccacgtcaagaatggtctcgagccatgtatcgca816AlaGluGlyTyrTyrHisValLysAsnGlyLeuGluProCysIleAla260265270cgtgcaaagtcctacgcaccatacgcagatatgatctggatggagacc864ArgAlaLysSerTyrAlaProTyrAlaAspMetIleTrpMetGluThr275280285ggcacccctgacctggagctcgctaagaagttcgctgaaggcgttcgc912GlyThrProAspLeuGluLeuAlaLysLysPheAlaGluGlyValArg290295300tctgagttcccagaccagctgctgtcctacaactgctccccatccttc960SerGluPheProAspGlnLeuLeuSerTyrAsnCysSerProSerPhe305310315320aactggtctgcacacctcgaggcagatgagatcgctaagttccagaag1008AsnTrpSerAlaHisLeuGluAlaAspGluIleAlaLysPheGlnLys325330335gaactcggcgcaatgggcttcaagttccagttcatcaccctcgcaggc1056GluLeuGlyAlaMetGlyPheLysPheGlnPheIleThrLeuAlaGly340345350ttccactccctcaactacggcatgttcgacctggcttacggatacgct1104PheHisSerLeuAsnTyrGlyMetPheAspLeuAlaTyrGlyTyrAla355360365cgcgaaggcatgacctccttcgttgacctgcagaaccgtgagttcaag1152ArgGluGlyMetThrSerPheValAspLeuGlnAsnArgGluPheLys370375380gcagctgaagagcgtggcttcaccgctgttaagcaccagcgtgaggtt1200AlaAlaGluGluArgGlyPheThrAlaValLysHisGlnArgGluVal385390395400ggcgcaggctacttcgaccagatcgcaaccaccgttgacccgaactct1248GlyAlaGlyTyrPheAspGlnIleAlaThrThrValAspProAsnSer405410415tctaccaccgctttgaagggttccactgaagaaggccagttccacaac1296SerThrThrAlaLeuLysGlySerThrGluGluGlyGlnPheHisAsn420425430<210>54<211>432<212>PRT<213>谷氨酸棒杆菌<400>54MetSerAsnValGlyLysProArgThrAlaGlnGluIleGlnGlnAsp151015TrpAspThrAsnProArgTrpAsnGlyIleThrArgAspTyrThrAla202530AspGlnValAlaAspLeuGlnGlySerValIleGluGluHisThrLeu354045AlaArgArgGlySerGluIleLeuTrpAspAlaValThrGlnGluGly505560AspGlyTyrIleAsnAlaLeuGlyAlaLeuThrGlyAsnGlnAlaVal65707580GlnGlnValArgAlaGlyLeuLysAlaValTyrLeuSerGlyTrpGln859095ValAlaGlyAspAlaAsnLeuSerGlyHisThrTyrProAspGlnSer100105110LeuTyrProAlaAsnSerValProSerValValArgArgIleAsnAsn115120125AlaLeuLeuArgSerAspGluIleAlaArgThrGluGlyAspThrSer130135140ValAspAsnTrpValValProIleValAlaAspGlyGluAlaGlyPhe145150155160GlyGlyAlaLeuAsnValTyrGluLeuGlnLysAlaMetIleAlaAla165170175GlyAlaAlaGlyThrHisTrpGluAspGlnLeuAlaSerGluLysLys180185190CysGlyHisLeuGlyGlyLysValLeuIleProThrGlnGlnHisIle195200205ArgThrLeuAsnSerAlaArgLeuAlaAlaAspValAlaAsnThrPro210215220ThrValValIleAlaArgThrAspAlaGluAlaAlaThrLeuIleThr225230235240SerAspValAspGluArgAspGlnProPheIleThrGlyGluArgThr245250255AlaGluGlyTyrTyrHisValLysAsnGlyLeuGluProCysIleAla260265270ArgAlaLysSerTyrAlaProTyrAlaAspMetIleTrpMetGluThr275280285GlyThrProAspLeuGluLeuAlaLysLysPheAlaGluGlyValArg290295300SerGluPheProAspGlnLeuLeuSerTyrAsnCysSerProSerPhe305310315320AsnTrpSerAlaHisLeuGluAlaAspGluIleAlaLysPheGlnLys325330335GluLeuGlyAlaMetGlyPheLysPheGlnPheIleThrLeuAlaGly340345350PheHisSerLeuAsnTyrGlyMetPheAspLeuAlaTyrGlyTyrAla355360365ArgGluGlyMetThrSerPheValAspLeuGlnAsnArgGluPheLys370375380AlaAlaGluGluArgGlyPheThrAlaValLysHisGlnArgGluVal385390395400GlyAlaGlyTyrPheAspGlnIleAlaThrThrValAspProAsnSer405410415SerThrThrAlaLeuLysGlySerThrGluGluGlyGlnPheHisAsn420425430<210>55<211>2217<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2217)<223>RXA02404K<400>55atgactgaacaggaactgttgtctgctcagactgccgacaacgctgga48MetThrGluGlnGluLeuLeuSerAlaGlnThrAlaAspAsnAlaGly151015actgacagcaccgaacgcgttgacgcgggcggaatgcaggttgcaaaa96ThrAspSerThrGluArgValAspAlaGlyGlyMetGlnValAlaLys202530gttctctacgactttgtaaccgaagcggtactccctcgcgtgggtgtg144ValLeuTyrAspPheValThrGluAlaValLeuProArgValGlyVal354045gatgcggaaaagttctggtccggattcgccgccatcgcccgggacctc192AspAlaGluLysPheTrpSerGlyPheAlaAlaIleAlaArgAspLeu505560accccacgcaaccgcgagctgcttgctcgccgcgatgaactgcagatg240ThrProArgAsnArgGluLeuLeuAlaArgArgAspGluLeuGlnMet65707580cttatcgacgactaccaccgcaacaactccggcaccatcgaccaagag288LeuIleAspAspTyrHisArgAsnAsnSerGlyThrIleAspGlnGlu859095gcgtacgaggatttcctcaaagaaatcggatacttggttgaggagcca336AlaTyrGluAspPheLeuLysGluIleGlyTyrLeuValGluGluPro100105110gaagctgcagaaatccgtacccaaaacgtcgatacggaaatctccagc384GluAlaAlaGluIleArgThrGlnAsnValAspThrGluIleSerSer115120125accgcaggacctcagctggttgttccaattctgaacgcacgcttcgcg432ThrAlaGlyProGlnLeuValValProIleLeuAsnAlaArgPheAla130135140ctgaacgctgccaatgctcgctggggttccctctacgatgcgttgtac480LeuAsnAlaAlaAsnAlaArgTrpGlySerLeuTyrAspAlaLeuTyr145150155160ggcaccaacgccatcccagaaactgatggcgctgaaaagggcaaggag528GlyThrAsnAlaIleProGluThrAspGlyAlaGluLysGlyLysGlu165170175tacaacccggtccgcggccagaaggtcatcgagtggggtcgtgaattc576TyrAsnProValArgGlyGlnLysValIleGluTrpGlyArgGluPhe180185190ctcgacagcgttgtcccactggacggtgcttcgcatgccgatgttgag624LeuAspSerValValProLeuAspGlyAlaSerHisAlaAspValGlu195200205aagtacaacateaccgatggaaagcttgcagcccacattggagatagc672LysTyrAsnIleThrAspGlyLysLeuAlaAlaHisIleGlyAspSer210215220gtctaccgactgaaaaaccgtgaatcctaccgtggcttcaccggcaac720ValTyrArgLeuLysAsnArgGluSerTyrArgGlyPheThrGlyAsn225230235240ttccttgatccagaagcaatcctgctggaaaccaacggcctgcacatc768PheLeuAspProGluAlaIleLeuLeuGluThrAsnGlyLeuHisIle245250255gagctgcagatcgatcctgtccacccaatcggcaaggcagacaagact816GluLeuGlnIleAspProValHisProIleGlyLysAlaAspLysThr260265270ggtctcaaagacatcgttttggaatctgcgatcaccacgatcatggac864GlyLeuLysAspIleValLeuGluSerAlaIleThrThrIleMetAsp275280285ttcgaagactccgttgcagctgttgatgctgaagacaagaccttaggt912PheGluAspSerValAlaAlaValAspAlaGluAspLysThrLeuGly290295300tactctaactggttcggactcaacaccggcgaactgaaagaagagatg960TyrSerAsnTrpPheGlyLeuAsnThrGlyGluLeuLysGluGluMet305310315320tccaagaacggacgcatcttcacccgtgagctcaacaaggaccgcgtc1008SerLysAsnGlyArgIlePheThrArgGluLeuAsnLysAspArgVal325330335tacattggccgcaatggtaccgagctggttctgcacggtcgttccctg1056TyrIleGlyArgAsnGlyThrGluLeuValLeuHisGlyArgSerLeu340345350ctgttcgtccgcaacgttggtcacctcatgcaaaacccatccatcttg1104LeuPheValArgAsnValGlyHisLeuMetGlnAsnProSerIleLeu355360365attgatggcgaggagatcttcgaaggcatcatggatgctgtcttgacc1152IleAspGlyGluGluIlePheGluGlyIleMetAspAlaValLeuThr370375380actgtttgtgccatcccaggaattgctccgcagaacaagatgcgcaat1200ThrValCysAlaIleProGlyIleAlaProGlnAsnLysMetArgAsn385390395400tcccgcaagggctccatctacatcgtgaagcctaagcagcacggccct1248SerArgLysGlySerIleTyrIleValLysProLysGlnHisGlyPro405410415gaagaagtcgcgttcaccaacgagctcttcggccgcgttgaggatctg1296GluGluValAlaPheThrAsnGluLeuPheGlyArgValGluAspLeu420425430cttgatctgccacgccacaccttgaaggttggtgttatggatgaggag1344LeuAspLeuProArgHisThrLeuLysValGlyValMetAspGluGlu435440445cgtcgcacgtccgtgaacctggatgccagcatcatggaagttgctgac1392ArgArgThrSerValAsnLeuAspAlaSerIleMetGluValAlaAsp450455460cgcttggcattcatcaacactggcttcctggaccgcaccggcgatgaa1440ArgLeuAlaPheIleAsnThrGlyPheLeuAspArgThrGlyAspGlu465470475480atccacacctccatggaagcaggcgccatggtgcgcaaggctgatatg1488IleHisThrSerMetGluAlaGlyAlaMetValArgLysAlaAspMet485490495cagaccgcaccgtggaagcaggcctacgagaacaacaacgttgatgca1536GlnThrAlaProTrpLysGlnAlaTyrGluAsnAsnAsnValAspAla500505510ggtattcagcgtggtcttcctggcaaggctcagatcggtaagggcatg1584GlyIleGlnArgGlyLeuProGlyLysAlaGlnIleGlyLysGlyMet515520525tgggcgatgactgaactcatggcagaaatgctggagaagaagatcggc1632TrpAlaMetThrGluLeuMetAlaGluMetLeuGluLysLysIleGly530535540cagccacgcgaaggcgccaacactgcatgggttccttcaccaactggt1680GlnProArgGluGlyAlaAsnThrAlaTrpValProSerProThrGly545550555560gcgacgctgcacgcaacgcactaccacttggttgatgtgttcaaggtt1728AlaThrLeuHisAlaThrHisTyrHisLeuValAspValPheLysVal555570575caagacgaactgcgtgctgccggccgccgcgacagcctgcgcaacatt1776GlnAspGluLeuArgAlaAlaGlyArgArgAspSerLeuArgAsnIle580585590ctcaccattccaaccgcaccaaacaccaattggtctgaggaagagaag1824LeuThrIleProThrAlaProAsnThrAsnTrpSerGluGluGluLys595600605aaggaagagatggacaacaactgccagtccatcctcggatacgttgtg1872LysGluGluMetAspAsnAsnCysGlnSerIleLeuGlyTyrValVal610615620cgctgggttgagcacggtgttggttgctccaaggttccagacatccat1920ArgTrpValGluHisGlyValGlyCysSerLysValProAspIleHis625630635640gacatcgacctcatggaagaccgcgcaacgctgcgtatttcctcgcag1968AspIleAspLeuMetGluAspArgAlaThrLeuArgIleSerSerGln645650655atgctggccaactggatccgccatgatgttgtctcgaaggagcaggtc2016MetLeuAlaAsnTrpIleArgHisAspValValSerLysGluGlnVal660665670ttggagtcactggaacgaatggcagtggtcgtcgacaagcaaaatgcg2064LeuGluSerLeuGluArgMetAlaValValValAspLysGlnAsnAla675680685ggcgacgaggcctaccgcgatatggcgccgaactacgacgcctccctc2112GlyAspGluAlaTyrArgAspMetAlaProAsnTyrAspAlaSerLeu690695700gccttccaggcggctaaggacttgattttcgaaggcaccaagtcccca2160AlaPheGlnAlaAlaLysAspLeuIlePheGluGlyThrLysSerPro705710715720tcgggctacaccgagcccatcttgcacgcacgccgccgcgagttcaaa2208SerGlyTyrThrGluProIleLeuHisAlaArgArgArgGluPheLys725730735gcaaaaaac2217AlaLysAsn<210>56<211>739<212>PRT<213>谷氨酸棒杆菌<400>56MetThrGluGlnGluLeuLeuSerAlaGlnThrAlaAspAsnAlaGly151015ThrAspSerThrGluArgValAspAlaGlyGlyMetGlnValAlaLys202530ValLeuTyrAspPheValThrGluAlaValLeuProArgValGlyVal354045AspAlaGluLysPheTrpSerGlyPheAlaAlaIleAlaArgAspLeu505560ThrProArgAsnArgGluLeuLeuAlaArgArgAspGluLeuGlnMet65707580LeuIleAspAspTyrHisArgAsnAsnSerGlyThrIleAspGlnGlu859095AlaTyrGluAspPheLeuLysGluIleGlyTyrLeuValGluGluPro100105110GluAlaAlaGluIleArgThrGlnAsnValAspThrGluIleSerSer115120125ThrAlaGlyProGlnLeuValValProIleLeuAsnAlaArgPheAla130135140LeuAsnAlaAlaAsnAlaArgTrpGlySerLeuTyrAspAlaLeuTyr145150155160GlyThrAsnAlaIleProGluThrAspGlyAlaGluLysGlyLysGlu165170175TyrAsnProValArgGlyGlnLysValIleGluTrpGlyArgGluPhe180185190LeuAspSerValValProLeuAspGlyAlaSerHisAlaAspValGlu195200205LysTyrAsnIleThrAspGlyLysLeuAlaAlaHisIleGlyAspSer210215220ValTyrArgLeuLysAsnArgGluSerTyrArgGlyPheThrGlyAsn225230235240PheLeuAspProGluAlaIleLeuLeuGluThrAsnGlyLeuHisIle245250255GluLeuGlnIleAspProValHisProIleGlyLysAlaAspLysThr260265270GlyLeuLysAspIleValLeuGluSerAlaIleThrThrIleMetAsp275280285PheGluAspSerValAlaAlaValAspAlaGluAspLysThrLeuGly290295300TyrSerAsnTrpPheGlyLeuAsnThrGlyGluLeuLysGluGluMet305310315320SerLysAsnGlyArgIlePheThrArgGluLeuAsnLysAspArgVal325330335TyrIleGlyArgAsnGlyThrGluLeuValLeuHisGlyArgSerLeu340345350LeuPheValArgAsnValGlyHisLeuMetGlnAsnProSerIleLeu355360365IleAspGlyGluGluIlePheGluGlyIleMetAspAlaValLeuThr370375380ThrValCysAlaIleProGlyIleAlaProGlnAsnLysMetArgAsn385390395400SerArgLysGlySerIleTyrIleValLysProLysGlnHisGlyPro405410415GluGluValAlaPheThrAsnGluLeuPheGlyArgValGluAspLeu420425430LeuAspLeuProArgHisThrLeuLysValGlyValMetAspGluGlu435440445ArgArgThrSerValAsnLeuAspAlaSerIleMetGluValAlaAsp450455460ArgLeuAlaPheIleAsnThrGlyPheLeuAspArgThrGlyAspGlu465470475480IleHisThrSerMetGluAlaGlyAlaMetValArgLysAlaAspMet485490495GlnThrAlaProTrpLysGlnAlaTyrGluAsnAsnAsnValAspAla500505510GlyIleGlnArgGlyLeuProGlyLysAlaGlnIleGlyLysGlyMet515520525TrpAlaMetThrGluLeuMetAlaGluMetLeuGluLysLysIleGly530535540GlnProArgGluGlyAlaAsnThrAlaTrpValProSerProThrGly545550555560AlaThrLeuHisAlaThrHisTyrHisLeuValAspValPheLysVal565570575GlnAspGluLeuArgAlaAlaGlyArgArgAspSerLeuArgAsnIle580585590LeuThrIleProThrAlaProAsnThrAsnTrpSerGluGluGluLys595600605LysGluGluMetAspAsnAsnCysGlnSerIleLeuGlyTyrValVal610615620ArgTrpValGluHisGlyValGlyCysSerLysValProAspIleHis625630635640AspIleAspLeuMetGluAspArgAlaThrLeuArgIleSerSerGln645650655MetLeuAlaAsnTrpIleArgHisAspValValSerLysGluGlnVal660665670LeuGluSerLeuGluArgMetAlaValValValAspLysGlnAsnAla675680685GlyAspGluAlaTyrArgAspMetAlaProAsnTyrAspAlaSerLeu690695700AlaPheGlnAlaAlaLysAspLeuIlePheGluGlyThrLysSerPro705710715720SerGlyTyrThrGluProIleLeuHisAlaArgArgArgGluPheLys725730735AlaLysAsn<210>57<211>1830<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1830)<223>RXA02591W<400>57atgactactgctgcaatcaggggccttcagggcgaggcgccgaccaag48MetThrThrAlaAlaIleArgGlyLeuGlnGlyGluAlaProThrLys151015aataaggaactgctgaactggatcgcagacgccgtcgagctcttccag96AsnLysGluLeuLeuAsnTrpIleAlaAspAlaValGluLeuPheGln202530cctgaggctgttgtgttcgttgatggatcccaggctgagtgggatcgc144ProGluAlaValValPheValAspGlySerGlnAlaGluTrpAspArg354045atggcggaggatcttgttgaagccggtaccctcatcaagctcaacgag192MetAlaGluAspLeuValGluAlaGlyThrLeuIleLysLeuAsnGlu505560gaaaagcgtccgaacagctacctagctcgttccaacccatctgacgtt240GluLysArgProAsnSerTyrLeuAlaArgSerAsnProSerAspVal65707580gcgcgcgttgagtcccgcaccttcatctgctccgagaaggaagaagat288AlaArgValGluSerArgThrPheIleCysSerGluLysGluGluAsp859095gctggcccaaccaacaactgggctccaccacaggcaatgaaggacgaa336AlaGlyProThrAsnAsnTrpAlaProProGlnAlaMetLysAspGlu100105110atgtccaagcattacgctggttccatgaaggggcgcaccatgtacgtc384MetSerLysHisTyrAlaGlySerMetLysGlyArgThrMetTyrVal115120125gtgcctttctgcatgggtccaatcagcgatccggaccctaagcttggt432ValProPheCysMetGlyProIleSerAspProAspProLysLeuGly130135140gtgcagctcactgactccgagtacgttgtcatgtccatgcgcatcatg480ValGlnLeuThrAspSerGluTyrValValMetSerMetArgIleMet145150155160acccgcatgggtattgaagcgctggacaagatcggcgcgaacggcagc528ThrArgMetGlyIleGluAlaLeuAspLysIleGlyAlaAsnGlySer165170175ttcgtcaggtgcctccactccgttggtgctcctttggagccaggccag576PheValArgCysLeuHisSerValGlyAlaProLeuGluProGlyGln180185190gaagacgttgcatggccttgcaacgacaccaagtacatcacccagttc624GluAspValAlaTrpProCysAsnAspThrLysTyrIleThrGlnPhe195200205ccagagaccaaggaaatttggtcctacggttccggctacggcggaaac672ProGluThrLysGluIleTrpSerTyrGlySerGlyTyrGlyGlyAsn210215220gcaatcctggcaaagaagtgctacgcactgcgtatcgcatctgtcatg720AlaIleLeuAlaLysLysCysTyrAlaLeuArgIleAlaSerValMer225230235240gctcgcgaagaaggatggatggctgagcacatgctcatcctgaagctg768AlaArgGluGluGlyTrpMetAlaGluHisMetLeuIleLeuLysLeu245250255atcaacccagagggcaaggcgtaccacatcgcagcagcattcccatct816IleAsnProGluGlyLysAlaTyrHisIleAlaAlaAlaPheProSer260265270gcttgtggcaagaccaacctcgccatgatcactccaaccatcccaggc864AlaCysGlyLysThrAsnLeuAlaMetIleThrProThrIleProGly275280285tggaccgctcaggttgttggcgacgacatcgcttggctgaagctgcgc912TrpThrAlaGlnValValGlyAspAspIleAlaTrpLeuLysLeuArg290295300gaggacggcctctacgcagttaacccagaaaatggtttcttcggtgtt960GluAspGlyLeuTyrAlaValAsnProGluAsnGlyPhePheGlyVal305310315320gctccaggcaccaactacgcatccaacccaatcgcgatgaagaccatg1008AlaProGlyThrAsnTyrAlaSerAsnProIleAlaMetLysThrMet325330335gaaccaggcaacaccctgttcaccaacgtggcactcaccgacgacggc1056GluProGlyAsnThrLeuPheThrAsnValAlaLeuThrAspAspGly340345350gacatctggtgggaaggcatggacggcgacgccccagctcacctcatt1104AspIleTrpTrpGluGlyMetAspGlyAspAlaProAlaHisLeuIle355360365gactggatgggcaacgactggaccccagagtccgacgaaaacgctgct1152AspTrpMetGlyAsnAspTrpThrProGluSerAspGluAsnAlaAla370375380caccctaactcccgttactgcgtagcaatcgaccagtccccagcagca1200HisProAsnSerArgTyrCysValAlaIleAspGlnSerProAlaAla385390395400gcacctgagttcaacgactgggaaggcgtcaagatcgacgcaatcctc1248AlaProGluPheAsnAspTrpGluGlyValLysIleAspAlaIleLeu405410415ttcggtggacgtcgcgcagacaccgtcccactggttacccagacctac1296PheGlyGlyArgArgAlaAspThrValProLeuValThrGlnThrTyr420425430gactgggagcacggcaccatggttggtgcactgctcgcatccggtcag1344AspTrpGluHisGlyThrMetValGlyAlaLeuLeuAlaSerGlyGln435440445accgcagcttccgcagaagcaaaggtcggcacactccgccacgaccca1392ThrAlaAlaSerAlaGluAlaLysValGlyThrLeuArgHisAspPro450455460atggcaatgctcccattcattggctacaacgctggtgaatacctgcag1440MetAlaMetLeuProPheIleGlyTyrAsnAlaGlyGluTyrLeuGln465470475480aactggattgacatgggtaacaagggtggcgacaagatgccatccatc1488AsnTrpIleAspMetGlyAsnLysGlyGlyAspLysMetProSerIle485490495ttcctggtcaactggttccgccgtggcgaagatggacgcttcctgtgg1536PheLeuValAsnTrpPheArgArgGlyGluAspGlyArgPheLeuTrp500505510cctggcttcggcgacaactctcgcgttctgaagtgggtcatcgaccgc1584ProGlyPheGlyAspAsnSerArgValLeuLysTrpValIleAspArg515520525atcgaaggccacgttggcgcagacgagaccgttgttggacacaccgct1632IleGluGlyHisValGlyAlaAspGluThrValValGlyHisThrAla530535540aaggccgaagacctcgacctcgacggcctcgacaccccaattgaggat1680LysAlaGluAspLeuAspLeuAspGlyLeuAspThrProIleGluAsp545550555560gtcaaggaagcactgaccgctcctgcagagcagtgggcaaacgacgtt1728ValLysGluAlaLeuThrAlaProAlaGluGlnTrpAlaAsnAspVal565570575gaagacaacgccgagtacctcactttcctcggaccacgtgttcctgca1776GluAspAsnAlaGluTyrLeuThrPheLeuGlyProArgValProAla580585590gaggttcacagccagttcgatgctctgaaggcccgcatttcagcagct1824GluValHisSerGlnPheAspAlaLeuLysAlaArgIleSerAlaAla595600605cacgct1830HisAla610<210>58<211>610<212>PRT<213>谷氨酸棒杆菌<400>58MetThrThrAlaAlaIleArgGlyLeuGlnGlyGluAlaProThrLys151015AsnLysGluLeuLeuAsnTrpIleAlaAspAlaValGluLeuPheGln202530ProGluAlaValValPheValAspGlySerGlnAlaGluTrpAspArg354045MetAlaGluAspLeuValGluAlaGlyThrLeuIleLysLeuAsnGlu505560GluLysArgProAsnSerTyrLeuAlaArgSerAsnProSerAspVal65707580AlaArgValGluSerArgThrPheIleCysSerGluLysGluGluAsp859095AlaGlyProThrAsnAsnTrpAlaProProGlnAlaMetLysAspGlu100105110MetSerLysHisTyrAlaGlySerMetLysGlyArgThrMetTyrVal115120125ValProPheCysMetGlyProIleSerAspProAspProLysLeuGly130135140ValGlnLeuThrAspSerGluTyrValValMetSerMetArgIleMet145150155160ThrArgMetGlyIleGluAlaLeuAspLysIleGlyAlaAsnGlySer165170175PheValArgCysLeuHisSerValGlyAlaProLeuGluProGlyGln180185190GluAspValAlaTrpProCysAsnAspThrLysTyrIleThrGlnPhe195200205ProGluThrLysGluIleTrpSerTyrGlySerGlyTyrGlyGlyAsn210215220AlaIleLeuAlaLysLysCysTyrAlaLeuArgIleAlaSerValMet225230235240AlaArgGluGluGlyTrpMetAlaGluHisMetLeuIleLeuLysLeu245250255IleAsnProGluGlyLysAlaTyrHisIleAlaAlaAlaPheProSer260265270AlaCysGlyLysThrAsnLeuAlaMetIleThrProThrIleProGly275280285TrpThrAlaGlnValValGlyAspAspIleAlaTrpLeuLysLeuArg290295300GluAspGlyLeuTyrAlaValAsnProGluAsnGlyPhePheGlyVal305310315320AlaProGlyThrAsnTyrAlaSerAsnProIleAlaMetLysThrMet325330335GluProGlyAsnThrLeuPheThrAsnValAlaLeuThrAspAspGly340345350AspIleTrpTrpGluGlyMetAspGlyAspAlaProAlaHisLeuIle355360365AspTrpMetGlyAsnAspTrpThrProGluSerAspGluAsnAlaAla370375380HisProAsnSerArgTyrCysValAlaIleAspGlnSerProAlaAla385390395400AlaProGluPheAsnAspTrpGluGlyValLysIleAspAlaIleLeu405410415PheGlyGlyArgArgAlaAspThrValProLeuValThrGlnThrTyr420425430AspTrpGluHisGlyThrMetValGlyAlaLeuLeuAlaSerGlyGln435440445ThrAlaAlaSerAlaGluAlaLysValGlyThrLeuArgHisAspPro450455460MetAlaMetLeuProPheIleGlyTyrAsnAlaGlyGluTyrLeuGln465470475480AsnTrpIleAspMetGlyAsnLysGlyGlyAspLysMetProSerIle485490495PheLeuValAsnTrpPheArgArgGlyGluAspGlyArgPheLeuTrp500505510ProGlyPheGlyAspAsnSerArgValLeuLysTrpValIleAspArg515520525IleGluGlyHisValGlyAlaAspGluThrValValGlyHisThrAla530535540LysAlaGluAspLeuAspLeuAspGlyLeuAspThrProIleGluAsp545550555560ValLysGluAlaLeuThrAlaProAlaGluGlnTrpAlaAsnAspVal565570575GluAspAsnAlaGluTyrLeuThrPheLeuGlyProArgValProAla580585590GluValHisSerGlnPheAspAlaLeuLysAlaArgIleSerAlaAla595600605HisAla610<210>59<211>825<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(825)<223>RXA02735E<400>59gtgagtccgactgcttgtctgaggagcttcgccacatggatccagatt48MetSerProThrAlaCysLeuArgSerPheAlaThrTrpIleGlnIle151015tgggctaccagcacgcactatccggcttgtccagcgtcaagctggaaa96TrpAlaThrSerThrHisTyrProAlaCysProAlaSerSerTrpLys202530ccgtctaaggagaaatacaacactatggttgatgtagtacgcgcacgc144ProSerLysGluLysTyrAsnThrMetValAspValValArgAlaArg354045gatactgaagatttggttgcacaggctgcctccaaattcattgaggtt192AspThrGluAspLeuValAlaGlnAlaAlaSerLysPheIleGluVal505560gttgaagcagcaactgccaataatggcaccgcacaggtagtgctcacc240ValGluAlaAlaThrAlaAsnAsnGlyThrAlaGlnValValLeuThr65707580ggtggtggcgccggcatcaagttgctggaaaagctcagcgttgatgcg288GlyGlyGlyAlaGlyIleLysLeuLeuGluLysLeuSerValAspAla859095gctgaccttgcctgggatcgcattcatgtgttcttcggcgatgagcgc336AlaAspLeuAlaTrpAspArgIleHisValPhePheGlyAspGluArg100105110aatgtccctgtcagtgattctgagtccaatgagggccaggctcgtgag384AsnValProValSerAspSerGluSerAsnGluGlyGlnAlaArgGlu115120125gcactgttgtccaaggtttctatccctgaagccaacattcacggatat432AlaLeuLeuSerLysValSerIleProGluAlaAsnIleHisGlyTyr130135140ggtotcggcgacgtagatcttgcagaggcagcccgcgcttacgaagct480GlyLeuGlyAspValAspLeuAlaGluAlaAlaArgAlaTyrGluAla145150155160gtgttggatgaattcgcaccaaacggctttgatcttcacctgctcggc528ValLeuAspGluPheAlaProAsnGlyPheAspLeuHisLeuLeuGly165170175atgggtggcgaaggccatatcaactccctgttccctcacaccgatgca576MetGlyGlyGluGlyHisIleAsnSerLeuPheProHisThrAspAla180185190gtcaaggaatcctccgcaaaggtcatcgcggtgtttgattcccctaag624ValLysGluSerSerAlaLysValIleAlaValPheAspSerProLys195200205cctccttcagagcgtgcaactctaacccttcctgcggttcactccgca672ProProSerGluArgAlaThrLeuThrLeuProAlaValHisSerAla210215220aagcgcgtgtggttgctggtttctggtgcggagaaggctgaggcagct720LysArgValTrpLeuLeuValSerGlyAlaGluLysAlaGluAlaAla225230235240gcggcgatcgtcaacggtgagcctgctgttgagtggcctgctgctgga768AlaAlaIleValAsnGlyGluProAlaValGluTrpProAlaAlaGly245250255gctaccggatctgaggaaacggtattgttcttggctgatgatgctgca816AlaThrGlySerGluGluThrValLeuPheLeuAlaAspAspAlaAla260265270ggaaatctc825GlyAsnLeu275<210>60<211>275<212>PRT<213>谷氨酸棒杆菌<400>60MetSerProThrAlaCysLeuArgSerPheAlaThrTrpIleGlnIle151015TrpAlaThrSerThrHisTyrProAlaCysProAlaSerSerTrpLys202530ProSerLysGluLysTyrAsnThrMetValAspValValArgAlaArg354045AspThrGluAspLeuValAlaGlnAlaAlaSerLysPheIleGluVal505560ValGluAlaAlaThrAlaAsnAsnGlyThrAlaGlnValValLeuThr65707580GlyGlyGlyAlaGlyIleLysLeuLeuGluLysLeuSerValAspAla859095AlaAspLeuAlaTrpAspArgIleHisValPhePheGlyAspGluArg100105110AsnValProValSerAspSerGluSerAsnGluGlyGlnAlaArgGlu115120125AlaLeuLeuSerLysValSerIleProGluAlaAsnIleHisGlyTyr130135140GlyLeuGlyAspValAspLeuAlaGluAlaAlaArgAlaTyrGluAla145150155160ValLeuAspGluPheAlaProAsnGlyPheAspLeuHisLeuLeuGly165170175MetGlyGlyGluGlyHisIleAsnSerLeuPheProHisThrAspAla180185190ValLysGluSerSerAlaLysValIleAlaValPheAspSerProLys195200205ProProSerGluArgAlaThrLeuThrLeuProAlaValHisSerAla210215220LysArgValTrpLeuLeuValSerGlyAlaGluLysAlaGluAlaAla225230235240AlaAlaIleValAsnGlyGluProAlaValGluTrpProAlaAlaGly245250255AlaThrGlySerGluGluThrValLeuPheLeuAlaAspAspAlaAla260265270GlyAsnLeu275<210>61<211>705<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(705)<223>RXA02735W<400>61atggttgatgtagtacgcgcacgcgatactgaagatttggttgcacag48MetValAspValValArgAlaArgAspThrGluAspLeuValAlaGln151015gctgcctccaaattcattgaggttgttgaagcagcaactgccaataat96AlaAlaSerLysPheIleGluValValGluAlaAlaThrAlaAsnAsn202530ggcaccgcacaggtagtgctcaccggtggtggcgccggcatcaagttg144GlyThrAlaGlnValValLeuThrGlyGlyGlyAlaGlyIleLysLeu354045ctggaaaagctcagcgttgatgcggctgaccttgcctgggatcgcatt192LeuGluLysLeuSerValAspAlaAlaAspLeuAlaTrpAspArgIle505560catgtgttcttcggcgatgagcgcaatgtccctgtcagtgattctgag240HisValPhePheGlyAspGluArgAsnValProValSerAspSerGlu65707580tccaatgagggccaggctcgtgaggcactgttgtccaaggtttctatc288SerAsnGluGlyGlnAlaArgGluAlaLeuLeuSerLysValSerIle859095cctgaagccaacattcacggatatggtctcggcgacgtagatcttgca336ProGluAlaAsnIleHisGlyTyrGlyLeuGlyAspValAspLeuAla100105110gaggcagcccgcgcttacgaagctgtgttggatgaattcgcaccaaac384GluAlaAlaArgAlaTyrGluAlaValLeuAspGluPheAlaProAsn115120125ggctttgatcttcacctgctcggcatgggtggcgaaggccatatcaac432GlyPheAspLeuHisLeuLeuGlyMetGlyGlyGluGlyHisIleAsn130135140tccctgttccctcacaccgatgcagtcaaggaatcctccgcaaaggtc480SerLeuPheProHisThrAspAlaValLysGluSerSerAlaLysVal145150155160atcgcggtgtttgattcccctaagcctccttcagagcgtgcaactcta528IleAlaValPheAspSerProLysProProSerGluArgAlaThrLeu165170175acccttcctgcggttcactccgcaaagcgcgtgtggttgctggtttct576ThrLeuProAlaValHisSerAlaLysArgValTrpLeuLeuValSer180185190ggtgcggagaaggctgaggcagctgcggcgatcgtcaacggtgagcct624GlyAlaGluLysAlaGluAlaAlaAlaAlaIleValAsnGlyGluPro195200205gctgttgagtggcctgctgctggagctaccggatctgaggaaacggta672AlaValGluTrpProAlaAlaGlyAlaThrGlySerGluGluThrVal210215220ttgttcttggctgatgatgctgcaggaaatctc705LeuPheLeuAlaAspAspAlaAlaGlyAsnLeu225230235<210>62<211>235<212>PRT<213>谷氨酸棒杆菌<400>62MetValAspValValArgAlaArgAspThrGluAspLeuValAlaGln151015AlaAlaSerLysPheIleGluValValGluAlaAlaThrAlaAsnAsn202530GlyThrAlaGlnValValLeuThrGlyGlyGlyAlaGlyIleLysLeu354045LeuGluLysLeuSerValAspAlaAlaAspLeuAlaTrpAspArgIle505560HisValPhePheGlyAspGluArgAsnValProValSerAspSerGlu65707580SerAsnGluGlyGlnAlaArgGluAlaLeuLeuSerLysValSerIle859095ProGluAlaAsnIleHisGlyTyrGlyLeuGlyAspValAspLeuAla100105110GluAlaAlaArgAlaTyrGluAlaValLeuAspGluPheAlaProAsn115120125GlyPheAspLeuHisLeuLeuGlyMetGlyGlyGluGlyHisIleAsn130135140SerLeuPheProHisThrAspAlaValLysGluSerSerAlaLysVal145150155160IleAlaValPheAspSerProLysProProSerGluArgAlaThrLeu165170175ThrLeuProAlaValHisSerAlaLysArgValTrpLeuLeuValSer180185190GlyAlaGluLysAlaGluAlaAlaAlaAlaIleValAsnGlyGluPro195200205AlaValGluTrpProAlaAlaGlyAlaThrGlySerGluGluThrVal210215220LeuPheLeuAlaAspAspAlaAlaGlyAsnLeu225230235<2l0>63<211>957<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(957)<223>RXA02736W<400>63atgatctttgaacttccggataccaccacccagcaaatttccaagacc48MetIlePheGluLeuProAspThrThrThrGlnGlnIleSerLysThr151015ctaactcgactgcgtgaatcgggcacccaggtcaccaccggccgagtg96LeuThrArgLeuArgGluSerGlyThrGlnValThrThrGlyArgVal202530ctcaccctcatcgtggtcactgactccgaaagcgatgtcgctgcagtt144LeuThrLeuIleValValThrAspSerGluSerAspValAlaAlaVal354045accgagtccaccaatgaagcctcgcgcgagcacccatctcgcgtgatc192ThrGluSerThrAsnGluAlaSerArgGluHisProSerArgValIle505560attttggtggttggcgataaaactgcagaaaacaaagttgacgcagaa240IleLeuValValGlyAspLysThrAlaGluAsnLysValAspAlaGlu65707580gtccgtatcggtggcgacgctggtgcttccgagatgatcatcatgcat288ValArgIleGlyGlyAspAlaGlyAlaSerGluMetIleIleMetHis859095ctcaacggacctgtcgctgacaagctccagtatgtcgtcacaccactg336LeuAsnGlyProValAlaAspLysLeuGlnTyrValValThrProLeu100105110ttgcttcctgacacccccatcgttgcttggtggccaggtgaatcacca384LeuLeuProAspThrProIleValAlaTrpTrpProGlyGluSerPro115120125aagaatccttcccaggacccaattggacgcatcgcacaacgacgcatc432LysAsnProSerGlnAspProIleGlyArgIleAlaGlnArgArgIle130135140actgatgctttgtacgaccgtgatgacgcactagaagatcgtgttgag480ThrAspAlaLeuTyrAspArgAspAspAlaLeuGluAspArgValGlu145150155160aactatcacccaggtgataccgacatgacgtgggcgcgccttacccag528AsnTyrHisProGlyAspThrAspMetThrTrpAlaArgLeuThrGln165170175tggcggggacttgttgcctcctcattggatcacccaccacacagcgaa576TrpArgGlyLeuValAlaSerSerLeuAspHisProProHisSerGlu180185190atcacttccgtgaggctgaccggtgcaagcggcagtacctcggtggat624IleThrSerValArgLeuThrGlyAlaSerGlySerThrSerValAsp195200205ttggctgcaggctggttggcgcggaggctgaaagtgcctgtgatccgc672LeuAlaAlaGlyTrpLeuAlaArgArgLeuLysValProValIleArg210215220gaggtgacagatgctcccaccgtgccaaccgatgagtttggtactcca720GluValThrAspAlaProThrValProThrAspGluPheGlyThrPro225230235240ctgctggctatccagcgcctggagatcgttcgcaccaccggctcgatc768LeuLeuAlaIleGlnArgLeuGluIleValArgThrThrGlySerIle245250255atcatcaccatctatgacgctcatacccttcaggtagagatgccggaa816IleIleThrIleTyrAspAlaHisThrLeuGlnValGluMetProGlu260265270tccggcaatgccccatcgctggtggctattggtcgtcgaagtgagtcc864SerGlyAsnAlaProSerLeuValAlaIleGlyArgArgSerGluSer275280285gactgcttgtctgaggagcttcgccacatggatccagatttgggctac912AspCysLeuSerGluGluLeuArgHisMetAspProAspLeuGlyTyr290295300cagcacgcactatccggcttgtccagcgtcaagctggaaaccgtc957GlnHisAlaLeuSerGlyLeuSerSerValLysLeuGluThrVal305310315<210>64<211>319<212>PRT<213>谷氨酸棒杆菌<400>64MetIlePheGluLeuProAspThrThrThrGlnGlnIleSerLysThr151015LeuThrArgLeuArgGluSerGlyThrGlnValThrThrGlyArgVal202530LeuThrLeuIleValValThrAspSerGluSerAspValAlaAlaVal354045ThrGluSerThrAsnGluAlaSerArgGluHisProSerArgValIle505560IleLeuValValGlyAspLysThrAlaGluAsnLysValAspAlaGlu65707580ValArgIleGlyGlyAspAlaGlyAlaSerGluMetIleIleMetHis859095LeuAsnGlyProValAlaAspLysLeuGlnTyrValValThrProLeu100105110LeuLeuProAspThrProIleValAlaTrpTrpProGlyGluSerPro115120125LysAsnProSerGlnAspProIleGlyArgIleAlaGlnArgArgIle130135140ThrAspAlaLeuTyrAspArgAspAspAlaLeuGluAspArgValGlu145150155160AsnTyrHisProGlyAspThrAspMetThrTrpAlaArgLeuThrGln165170175TrpArgGlyLeuValAlaSerSerLeuAspHisProProHisSerGlu180185190IleThrSerValArgLeuThrGlyAlaSerGlySerThrSerValAsp195200205LeuAlaAlaGlyTrpLeuAlaArgArgLeuLysValProValIleArg210215220GluValThrAspAlaProThrValProThrAspGluPheGlyThrPro225230235240LeuLeuAlaIleGlnArgLeuGluIleValArgThrThrGlySerIle245250255IleIleThrIleTyrAspAlaHisThrLeuGlnValGluMetProGlu260265270SerGlyAsnAlaProSerLeuValAlaIleGlyArgArgSerGluSer275280285AspCysLeuSerGluGluLeuArgHisMetAspProAspLeuGlyTyr290295300GlnHisAlaLeuSerGlyLeuSerSerValLysLeuGluThrVal305310315<210>65<211>1542<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1542)<223>RXA02737W<400>65gtgagcacaaacacgaccccctccagctggacaaacccactgcgcgac48MetSerThrAsnThrThrProSerSerTrpThrAsnProLeuArgAsp151015ccgcaggataaacgactcccccgcatcgctggcccttccggcatggtg96ProGlnAspLysArgLeuProArgIleAlaGlyProSerGlyMetVal202530atcttcggtgtcactggcgacttggctcgaaagaagctgctccccgcc144IlePheGlyValThrGlyAspLeuAlaArgLysLysLeuLeuProAla354045atttatgatctagcaaaccgcggattgctgcccccaggattctcgttg192IleTyrAspLeuAlaAsnArgGlyLeuLeuProProGlyPheSerLeu505560gtaggttacggccgccgcgaatggtccaaagaagactttgaaaaatac240ValGlyTyrGlyArgArgGluTrpSerLysGluAspPheGluLysTyr65707580gtacgcgatgccgcaagtgctggtgctcgtacggaattccgtgaaaat288ValArgAspAlaAlaSerAlaGlyAlaArgThrGluPheArgGluAsn859095gtttgggagcgcctcgccgagggtatggaatttgttcgcggcaacttt336ValTrpGluArgLeuAlaGluGlyMetGluPheValArgGlyAsnPhe100105110gatgatgatgcagctttcgacaacctcgctgcaacactcaagcgcatc384AspAspAspAlaAlaPheAspAsnLeuAlaAlaThrLeuLysArgIle115120125gacaaaacccgcggcaccgccggcaactgggcttactacctgtccatt432AspLysThrArgGlyThrAlaGlyAsnTrpAlaTyrTyrLeuSerIle130135140ccaccagattccttcacagcggtctgccaccagctggagcgttccggc480ProProAspSerPheThrAlaValCysHisGlnLeuGluArgSerGly145150155160atggctgaatccaccgaagaagcatggcgccgcgtgatcatcgagaag528MetAlaGluSerThrGluGluAlaTrpArgArgValIleIleGluLys165170175cctttcggccacaacctcgaatccgcacacgagctcaaccagctggtc576ProPheGlyHisAsnLeuGluSerAlaHisGluLeuAsnGlnLeuVal180185190aacgcagtcttcccagaatcttctgtgttccgcatcgaccactatttg624AsnAlaValPheProGluSerSerValPheArgIleAspHisTyrLeu195200205ggcaaggaaacagttcaaaacatcctggctctgcgttttgctaaccag672GlyLysGluThrValGlnAsnIleLeuAlaLeuArgPheAlaAsnGln210215220ctgtttgagccactgtggaactccaactacgttgaccacgtccagatc720LeuPheGluProLeuTrpAsnSerAsnTyrValAspHisValGlnIle225230235240accatggctgaagatattggcttgggtggacgtgctggttactacgac768ThrMetAlaGluAspIleGlyLeuGlyGlyArgAlaGlyTyrTyrAsp245250255ggcatcggcgcagcccgcgacgtcatccagaaccacctgatccagctc816GlyIleGlyAlaAlaArgAspValIleGlnAsnHisLeuIleGlnLeu260265270ttggctctggttgccatggaagaaccaatttctttcgtgccagcgcag864LeuAlaLeuValAlaMetGluGluProIleSerPheValProAlaGln275280285ctgcaggcagaaaagatcaaggtgctctctgcgacaaagccgtgctac912LeuGlnAlaGluLysIleLysValLeuSerAlaThrLysProCysTyr290295300ccattggataaaacctccgctcgtggtcagtacgctgccggttggcag960ProLeuAspLysThrSerAlaArgGlyGlnTyrAlaAlaGlyTrpGln305310315320ggctctgagttagtcaagggacttcgcgaagaagatggcttcaaccct1008GlySerGluLeuValLysGlyLeuArgGluGluAspGlyPheAsnPro325330335gagtccaccactgagacttttgcggcttgtaccttagagatcacgtct1056GluSerThrThrGluThrPheAlaAlaCysThrLeuGluIleThrSer340345350cgtcgctgggctggtgtgccgttctacctgcgcaccggtaagcgtctt1104ArgArgTrpAlaGlyValProPheTyrLeuArgThrGlyLysArgLeu355360365ggtcgccgtgttactgagattgccgtggtgtttaaagacgcaccacac1152GlyArgArgValThrGluIleAlaValValPheLysAspAlaProHis370375380cagcctttcgacggcgacatgactgtatcccttggccaaaacgccatc1200GlnProPheAspGlyAspMetThrValSerLeuGlyGlnAsnAlaIle385390395400gtgattcgcgtgcagcctgatgaaggtgtgctcatccgcttcggttcc1248ValIleArgValGlnProAspGluGlyValLeuIleArgPheGlySer405410415aaggttccaggttctgccatggaagtccgtgacgtcaacatggacttc1296LysValProGlySerAlaMetGluValArgAspValAsnMetAspPhe420425430tcctactcagaatccttcactgaagaatcacctgaagcatacgagcgc1344SerTyrSerGluSerPheThrGluGluSerProGluAlaTyrGluArg435440445ctcattttggatgcgctgttagatgaatccagcctcttccctaccaac1392LeuIleLeuAspAlaLeuLeuAspGluSerSerLeuPheProThrAsn450455460gaggaagtggaactgagctggaagattctggatccaattcttgaagca1440GluGluValGluLeuSerTrpLysIleLeuAspProIleLeuGluAla465470475480tgggatgccgatggagaaccagaggattacccagcgggtacgtggggt1488TrpAspAlaAspGlyGluProGluAspTyrProAlaGlyThrTrpGly485490495ccaaagagcgctgatgaaatgctttcccgcaacggtcacacctggcgc1536ProLysSerAlaAspGluMetLeuSerArgAsnGlyHisThrTrpArg500505510aggcca1542ArgPro<210>66<211>514<212>PRT<213>谷氨酸棒杆菌<400>66MetSerThrAsnThrThrProSerSerTrpThrAsnProLeuArgAsp151015ProGlnAspLysArgLeuProArgIleAlaGlyProSerGlyMetVal202530IlePheGlyValThrGlyAspLeuAlaArgLysLysLeuLeuProAla354045IleTyrAspLeuAlaAsnArgGlyLeuLeuProProGlyPheSerLeu505560ValGlyTyrGlyArgArgGluTrpSerLysGluAspPheGluLysTyr65707580ValArgAspAlaAlaSerAlaGlyAlaArgThrGluPheArgGluAsn859095ValTrpGluArgLeuAlaGluGlyMetGluPheValArgGlyAsnPhe100105110AspAspAspAlaAlaPheAspAsnLeuAlaAlaThrLeuLysArgIle115120125AspLysThrArgGlyThrAlaGlyAsnTrpAlaTyrTyrLeuSerIle130135140ProProAspSerPheThrAlaValCysHisGlnLeuGluArgSerGly145150155160MetAlaGluSerThrGluGluAlaTrpArgArgValIleIleGluLys165170175ProPheGlyHisAsnLeuGluSerAlaHisGluLeuAsnGlnLeuVal180185190AsnAlaValPheProGluSerSerValPheArgIleAspHisTyrLeu195200205GlyLysGluThrValGlnAsnIleLeuAlaLeuArgPheAlaAsnGln210215220LeuPheGluProLeuTrpAsnSerAsnTyrValAspHisValGlnIle225230235240ThrMetAlaGluAspIleGlyLeuGlyGlyArgAlaGlyTyrTyrAsp245250255GlyIleGlyAlaAlaArgAspValIleGlnAsnHisLeuIleGlnLeu260265270LeuAlaLeuValAlaMetGluGluProIleSerPheValProAlaGln275280285LeuGlnAlaGluLysIleLysValLeuSerAlaThrLysProCysTyr290295300ProLeuAspLysThrSerAlaArgGlyGlnTyrAlaAlaGlyTrpGln305310315320GlySerGluLeuValLysGlyLeuArgGluGluAspGlyPheAsnPro325330335GluSerThrThrGluThrPheAlaAlaCysThrLeuGluIleThrSer340345350ArgArgTrpAlaGlyValProPheTyrLeuArgThrGlyLysArgLeu355360365GlyArgArgValThrGluIleAlaValValPheLysAspAlaProHis370375380GlnProPheAspGlyAspMetThrValSerLeuGlyGlnAsnAlaIle385390395400ValIleArgValGlnProAspGluGlyValLeuIleArgPheGlySer405410415LysValProGlySerAlaMetGluValArgAspValAsnMetAspPhe420425430SerTyrSerGluSerPheThrGluGluSerProGluAlaTyrGluArg435440445LeuIleLeuAspAlaLeuLeuAspGluSerSerLeuPheProThrAsn450455460GluGluValGluLeuSerTrpLysIleLeuAspProIleLeuGluAla465470475480TrpAspAlaAspGlyGluProGluAspTyrProAlaGlyThrTrpGly485490495ProLysSerAlaAspGluMetLeuSerArgAsnGlyHisThrTrpArg500505510ArgPro<210>67<211>1080<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1080)<223>RXA02738W<400>67atgtctcacattgatgatcttgcacagctcggcacttccacttggctc48MetSerHisIleAspAspLeuAlaGlnLeuGlyThrSerThrTrpLeu151015gacgacctctcccgcgagcgcattacttccggcaatctcagccaggtt96AspAspLeuSerArgGluArgIleThrSerGlyAsnLeuSerGlnVal202530attgaggaaaagtctgtagtcggtgtcaccaccaacccagctattttc144IleGluGluLysSerValValGlyValThrThrAsnProAlaIlePhe354045gcagcagcaatgtccaagggcgattcctacgacgctcagatcgcagag192AlaAlaAlaMetSerLysGlyAspSerTyrAspAlaGlnIleAlaGlu505560ctcaaggccgctggcgcatctgttgaccaggctgtttacgccatgagc240LeuLysAlaAlaGlyAlaSerValAspGlnAlaValTyrAlaMetSer65707580atcgacgacgttcgcaatgcttgtgatctgttcaccggcatcttcgag288IleAspAspValArgAsnAlaCysAspLeuPheThrGlyIlePheGlu859095tcctccaacggctacgacggccgcgtgtccatcgaggttgacccacgt336SerSerAsnGlyTyrAspGlyArgValSerIleGluValAspProArg100105110atctctgctgaccgcgacgcaaccctggctcaggccaaggagctgtgg384IleSerAlaAspArgAspAlaThrLeuAlaGlnAlaLysGluLeuTrp115120125gcaaaggttgatcgtccaaacgtcatgatcaagatccctgcaacccca432AlaLysValAspArgProAsnValMetIleLysIleProAlaThrPro130135140ggttctttgccagcaatcaccgacgctttggctgagggcatcagcgtt480GlySerLeuProAlaIleThrAspAlaLeuAlaGluGlyIleSerVal145150155160aacgtcaccttgatcttctccgttgctcgctaccgcgaggtcatcgct528AsnValThrLeuIlePheSerValAlaArgTyrArgGluValIleAla165170175gcgttcatcgagggcatcaagcaggctgctgcaaacggccacgacgtc576AlaPheIleGluGlyIleLysGlnAlaAlaAlaAsnGlyHisAspVal180185190tccaagatccactctgtggcttccttcttcgtctcccgcgtcgacgtt624SerLysIleHisSerValAlaSerPhePheValSerArgValAspVal195200205gagatcgacaagcgcctcgaggcaatcggatccgatgaggctttggct672GluIleAspLysArgLeuGluAlaIleGlySerAspGluAlaLeuAla210215220ctgcgcggcaaggcaggcgttgccaacgctcagcgcgcttacgctgtg720LeuArgGlyLysAlaGlyValAlaAsnAlaGlnArgAlaTyrAlaVal225230235240tacaaggagcttttcgacgccgccgagctgcctgaaggtgccaacact768TyrLysGluLeuPheAspAlaAlaGluLeuProGluGlyAlaAsnThr245250255cagcgcccactgtgggcatccaccggcgtgaagaaccctgcgtacgct816GlnArgProLeuTrpAlaSerThrGlyValLysAsnProAlaTyrAla260265270gcaactctttacgtttccgagctggctggtccaaacaccgtcaacacc864AlaThrLeuTyrValSerGluLeuAlaGlyProAsnThrValAsnThr275280285atgccagaaggcaccatcgacgcggttctggagcagggcaacctgcac912MetProGluGlyThrIleAspAlaValLeuGluGlnGlyAsnLeuHis290295300ggtgacaccctgtccaactccgcggcagaagctgacgctgtgttctcc960GlyAspThrLeuSerAsnSerAlaAlaGluAlaAspAlaValPheSer305310315320cagcttgaggctctgggcgttgacttggcagatgtcttccaggtcctg1008GlnLeuGluAlaLeuGlyValAspLeuAlaAspValPheGlnValLeu325330335gagaccgagggtgtggacaagttcgttgcttcttggagcgaactgctt1056GluThrGluGlyValAspLysPheValAlaSerTrpSerGluLeuLeu340345350gagtccatggaagctcgcctgaag1080GluSerMetGluAlaArgLeuLys355360<210>68<211>360<212>PRT<213>谷氨酸棒杆菌<400>68MetSerHisIleAspAspLeuAlaGlnLeuGlyThrSerThrTrpLeu151015AspAspLeuSerArgGluArgIleThrSerGlyAsnLeuSerGlnVal202530IleGluGluLysSerValValGlyValThrThrAsnProAlaIlePhe354045AlaAlaAlaMetSerLysGlyAspSerTyrAspAlaGlnIleAlaGlu505560LeuLysAlaAlaGlyAlaSerValAspGlnAlaValTyrAlaMetSer65707580IleAspAspValArgAsnAlaCysAspLeuPheThrGlyIlePheGlu859095SerSerAsnGlyTyrAspGlyArgValSerIleGluValAspProArg100105110IleSerAlaAspArgAspAlaThrLeuAlaGlnAlaLysGluLeuTrp115120125AlaLysValAspArgProAsnValMetIleLysIleProAlaThrPro130135140GlySerLeuProAlaIleThrAspAlaLeuAlaGluGlyIleSerVal145150155160AsnValThrLeuIlePheSerValAlaArgTyrArgGluValIleAla165170175AlaPheIleGluGlyIleLysGlnAlaAlaAlaAsnGlyHisAspVal180185190SerLysIleHisSerValAlaSerPhePheValSerArgValAspVal195200205GluIleAspLysArgLeuGluAlaIleGlySerAspGluAlaLeuAla210215220LeuArgGlyLysAlaGlyValAlaAsnAlaGlnArgAlaTyrAlaVal225230235240TyrLysGluLeuPheAspAlaAlaGluLeuProGluGlyAlaAsnThr245250255GlnArgProLeuTrpAlaSerThrGlyValLysAsnProAlaTyrAla260265270AlaThrLeuTyrValSerGluLeuAlaGlyProAsnThrValAsnThr275280285MetProGluGlyThrIleAspAlaValLeuGluGlnGlyAsnLeuHis290295300GlyAspThrLeuSerAsnSerAlaAlaGluAlaAspAlaValPheSer305310315320GlnLeuGluAlaLeuGlyValAspLeuAlaAspValPheGlnValLeu325330335GluThrGluGlyValAspLysPheValAlaSerTrpSerGluLeuLeu340345350GluSerMetGluAlaArgLeuLys355360<210>69<211>2100<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2100)<223>RXA02739K<400>69ttgaccaccttgacgctgtcacctgaacttcaggcgctcactgtacgc48MetThrThrLeuThrLeuSerProGluLeuGlnAlaLeuThrValArg151015aattacccctctgattggtccgatgtggacaccaaggctgtagacact96AsnTyrProSerAspTrpSerAspValAspThrLysAlaValAspThr202530gttcgtgtcctcgctgcagacgctgtagaaaactgtggctccggccac144ValArgValLeuAlaAlaAspAlaValGluAsnCysGlySerGlyHis354045ccaggcaccgcaatgagcctggctccccttgcatacaccttgtaccag192ProGlyThrAlaMetSerLeuAlaProLeuAlaTyrThrLeuTyrGln505560cgggttatgaacgtagatccacaggacaccaactgggcaggccgtgac240ArgValMetAsnValAspProGlnAspThrAsnTrpAlaGlyArgAsp65707580cgcttcgttctttcttgtggccactcctctttgacccagtacatccag288ArgPheValLeuSerCysGlyHisSerSerLeuThrGlnTyrIleGln859095ctttacttgggtggattcggccttgagatggatgacctgaaggctctg336LeuTyrLeuGlyGlyPheGlyLeuGluMetAspAspLeuLysAlaLeu100105110cgcacctgggattccttgaccccaggacaccctgagtaccgccacacc384ArgThrTrpAspSerLeuThrProGlyHisProGluTyrArgHisThr115120125aagggcgttgagatcaccactggccctcttggccagggtcttgcatct432LysGlyValGluIleThrThrGlyProLeuGlyGlnGlyLeuAlaSer130135140gcagttggtatggccatggctgctcgtcgtgagcgtggcctattcgac480AlaValGlyMetAlaMetAlaAlaArgArgGluArgGlyLeuPheAsp145150155160ccaaccgctgctgagggcgaatccccattcgaccaccacatctacgtc528ProThrAlaAlaGluGlyGluSerProPheAspHisHisIleTyrVal165170175attgcttctgatggtgacctgcaggaaggtgtcacctctgaggcatcc576IleAlaSerAspGlyAspLeuGlnGluGlyValThrSerGluAlaSer180185190tccatcgctggcacccagcagctgggcaacctcatcgtgttctgggat624SerIleAlaGlyThrGlnGlnLeuGlyAsnLeuIleValPheTrpAsp195200205gacaaccgcatctccatcgaagacaacactgagatcgctttcaacgag672AspAsnArgIleSerIleGluAspAsnThrGluIleAlaPheAsnGlu210215220gacgttgttgctcgttacaaggcttacggctggcagaccattgaggtt720AspValValAlaArgTyrLysAlaTyrGlyTrpGlnThrIleGluVal225230235240gaggctggcgaggacgttgcagcaatcgaagctgcagtggctgaggct768GluAlaGlyGluAspValAlaAlaIleGluAlaAlaValAlaGluAla245250255aagaaggacaccaagcgacctaccttcatccgcgttcgcaccatcatc816LysLysAspThrLysArgProThrPheIleArgValArgThrIleIle260265270ggcttcccagctccaactatgatgaacaccggtgctgtgcacggtgct864GlyPheProAlaProThrMetMetAsnThrGlyAlaValHisGlyAla275280285gctcttggcgcagctgaggttgcagcaaccaagactgagcttggattc912AlaLeuGlyAlaAlaGluValAlaAlaThrLysThrGluLeuGlyPhe290295300gatcctgaggctcacttcgcgatcgacgatgaggttatcgctcacacc960AspProGluAlaHisPheAlaIleAspAspGluValIleAlaHisThr305310315320cgctccctcgcagagcgcgctgcacagaagaaggctgcatggcaggtc1008ArgSerLeuAlaGluArgAlaAlaGlnLysLysAlaAlaTrpGlnVal325330335aagttcgatgagtgggcagctgccaaccctgagaacaaggctctgttc1056LysPheAspGluTrpAlaAlaAlaAsnProGluAsnLysAlaLeuPhe340345350gatcgcctgaactcccgtgagcttccagcgggctacgctgacgagctc1104AspArgLeuAsnSerArgGluLeuProAlaGlyTyrAlaAspGluLeu355360365ccaacatgggatgcagatgagaagggcgtcgcaactcgtaaggcttcc1152ProThrTrpAspAlaAspGluLysGlyValAlaThrArgLysAlaSer370375380gaggctgcacttcaggcactgggcaagacccttcctgagctgtggggc1200GluAlaAlaLeuGlnAlaLeuGlyLysThrLeuProGluLeuTrpGly385390395400ggttccgctgacctcgcaggttccaacaacaccgtgatcaagggctcc1248GlySerAlaAspLeuAlaGlySerAsnAsnThrValIleLysGlySer405410415ccttccttcggccctgagtccatctccaccgagacctggtctgctgag1296ProSerPheGlyProGluSerIleSerThrGluThrTrpSerAlaGlu420425430ccttacggccgtaacctgcacttcggtatccgtgagcacgctatggga1344ProTyrGlyArgAsnLeuHisPheGlyIleArgGluHisAlaMetGly435440445tccatcctcaacggcatttccctccacggtggcacccgcccatacggc1392SerIleLeuAsnGlyIleSerLeuHisGlyGlyThrArgProTyrGly450455460ggaaccttcctcatcttctccgactacatgcgtcctgcagttcgtctt1440GlyThrPheLeuIlePheSerAspTyrMetArgProAlaValArgLeu465470475480gcagctctcatggagaccgacgcttactacgtctggacccacgactcc1488AlaAlaLeuMetGluThrAspAlaTyrTyrValTrpThrHisAspSer485490495atcggtctgggcgaagatggcccaacccaccagcctgttgaaaccttg1536IleGlyLeuGlyGluAspGlyProThrHisGlnProValGluThrLeu500505510gctgcactgcgcgccatcccaggtctgtccgtcctgcgtcctgcagat1584AlaAlaLeuArgAlaIleProGlyLeuSerValLeuArgProAlaAsp515520525gcgaacgagaccgcccaggcttgggctgcagcacttgagtacaaggaa1632AlaAsnGluThrAlaGlnAlaTrpAlaAlaAlaLeuGluTyrLysGlu530535540ggccctaagggtcttgcactgacccgccagaacgttcctgttctggaa1680GlyProLysGlyLeuAlaLeuThrArgGlnAsnValProValLeuGlu545550555560ggcaccaaggagaaggctgctgaaggcgttcgccgcggtggctacgtc1728GlyThrLysGluLysAlaAlaGluGlyValArgArgGlyGlyTyrVal565570575ctggttgagggttccaaggaaaccccagatgtgatcctcatgggctcc1776LeuValGluGlySerLysGluThrProAspValIleLeuMetGlySer580585590ggctccgaggttcagcttgcagttaacgctgcgaaggctctggaagct1824GlySerGluValGlnLeuAlaValAsnAlaAlaLysAlaLeuGluAla595600605gagggcgttgcagctcgcgttgtttccgttccttgcatggattggttc1872GluGlyValAlaAlaArgValValSerValProCysMetAspTrpPhe610615620caggagcaggacgcagagtacatcgagtccgttctgcctgcagctgtg1920GlnGluGlnAspAlaGluTyrIleGluSerValLeuProAlaAlaVal625630635640accgctcgtgtgtctgttgaagctggcatcgcaatgccttggtaccgc1968ThrAlaArgValSerValGluAlaGlyIleAlaMetProTrpTyrArg645650655ttcttgggcacccagggccgtgctgtctcccttgagcacttcggtgct2016PheLeuGlyThrGlnGlyArgAlaValSerLeuGluHisPheGlyAla660665670tctgcggattaccagaccctgtttgagaagttcggcatcaccaccgat2064SerAlaAspTyrGlnThrLeuPheGluLysPheGlyIleThrThrAsp675680685gcagtcgtggcagcggccaaggactccattaacggt2100AlaValValAlaAlaAlaLysAspSerIleAsnGly690695700<210>70<211>700<212>PRT<213>谷氨酸棒杆菌<400>70MetThrThrLeuThrLeuSerProGluLeuGlnAlaLeuThrValArg151015AsnTyrProSerAspTrpSerAspValAspThrLysAlaValAspThr202530ValArgValLeuAlaAlaAspAlaValGluAsnCysGlySerGlyHis354045ProGlyThrAlaMetSerLeuAlaProLeuAlaTyrThrLeuTyrGln505560ArgValMetAsnValAspProGlnAspThrAsnTrpAlaGlyArgAsp65707580ArgPheValLeuSerCysGlyHisSerSerLeuThrGlnTyrIleGln859095LeuTyrLeuGlyGlyPheGlyLeuGluMetAspAspLeuLysAlaLeu100105110ArgThrTrpAspSerLeuThrProGlyHisProGluTyrArgHisThr115120125LysGlyValGluIleThrThrGlyProLeuGlyGlnGlyLeuAlaSer130135140AlaValGlyMetAlaMetAlaAlaArgArgGluArgGlyLeuPheAsp145150155160ProThrAlaAlaGluGlyGluSerProPheAspHisHisIleTyrVal165170175IleAlaSerAspGlyAspLeuGlnGluGlyValThrSerGluAlaSer180185190SerIleAlaGlyThrGlnGlnLeuGlyAsnLeuIleValPheTrpAsp195200205AspAsnArgIleSerIleGluAspAsnThrGluIleAlaPheAsnGlu210215220AspValValAlaArgTyrLysAlaTyrGlyTrpGlnThrIleGluVal225230235240GluAlaGlyGluAspValAlaAlaIleGluAlaAlaValAlaGluAla245250255LysLysAspThrLysArgProThrPheIleArgValArgThrIleIle260265270GlyPheProAlaProThrMetMetAsnThrGlyAlaValHisGlyAla275280285AlaLeuGlyAlaAlaGluValAlaAlaThrLysThrGluLeuGlyPhe290295300AspProGluAlaHisPheAlaIleAspAspGluValIleAlaHisThr305310315320ArgSerLeuAlaGluArgAlaAlaGlnLysLysAlaAlaTrpGlnVal325330335LysPheAspGluTrpAlaAlaAlaAsnProGluAsnLysAlaLeuPhe340345350AspArgLeuAsnSerArgGluLeuProAlaGlyTyrAlaAspGluLeu355360365ProThrTrpAspAlaAspGluLysGlyValAlaThrArgLysAlaSer370375380GluAlaAlaLeuGlnAlaLeuGlyLysThrLeuProGluLeuTrpGly385390395400GlySerAlaAspLeuAlaGlySerAsnAsnThrValIleLysGlySer405410415ProSerPheGlyProGluSerIleSerThrGluThrTrpSerAlaGlu420425430ProTyrGlyArgAsnLeuHisPheGlyIleArgGluHisAlaMetGly435440445SerIleLeuAsnGlyIleSerLeuHisGlyGlyThrArgProTyrGly450455460GlyThrPheLeuIlePheSerAspTyrMetArgProAlaValArgLeu465470475480AlaAlaLeuMetGluThrAspAlaTyrTyrValTrpThrHisAspSer485490495IleGlyLeuGlyGluAspGlyProThrHisGlnProValGluThrLeu500505510AlaAlaLeuArgAlaIleProGlyLeuSerValLeuArgProAlaAsp515520525AlaAsnGluThrAlaGlnAlaTrpAlaAlaAlaLeuGluTyrLysGlu530535540GlyProLysGlyLeuAlaLeuThrArgGlnAsnValProValLeuGlu545550555560GlyThrLysGluLysAlaAlaGluGlyValArgArgGlyGlyTyrVal565570575LeuValGluGlySerLysGluThrProAspValIleLeuMetGlySer580585590GlySerGluValGlnLeuAlaValAsnAlaAlaLysAlaLeuGluAla595600605GluGlyValAlaAlaArgValValSerValProCysMetAspTrpPhe610615620GlnGluGlnAspAlaGluTyrIleGluSerValLeuProAlaAlaVal625630635640ThrAlaArgValSerValGluAlaGlyIleAlaMetProTrpTyrArg645650655PheLeuGlyThrGlnGlyArgAlaValSerLeuGluHisPheGlyAla660665670SerAlaAspTyrGlnThrLeuPheGluLysPheGlyIleThrThrAsp675680685AlaValValAlaAlaAlaLysAspSerIleAsnGly690695700<210>71<211>705<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(705)<223>RXA02910K<400>71gtggagattcgttggttggaaggctttatcgcggtcgcggaagaattg48MetGluIleArgTrpLeuGluGlyPheIleAlaValAlaGluGluLeu151015cactttagtaatgctgcgattcgtttggggatgccgcaatcgccgttg96HisPheSerAsnAlaAlaIleArgLeuGlyMetProGlnSerProLeu202530agtcagttgatccggcggttggagtcggagttggggcagaagcttttt144SerGlnLeuIleArgArgLeuGluSerGluLeuGlyGlnLysLeuPhe354045gatcgcagtacccggtcggtggagttaactgccgcgggtcgggcgttt192AspArgSerThrArgSerValGluLeuThrAlaAlaGlyArgAlaPhe505560ttgccacatgccagggggattgtggcgagcgctgcggtggcgagggaa240LeuProHisAlaArgGlyIleValAlaSerAlaAlaValAlaArgGlu65707580gctgtgaatgctgccgagggggagatcgttggtgttgttcgcattggt288AlaValAsnAlaAlaGluGlyGluIleValGlyValValArgIleGly859095ttttctggtgtgctgaactattccacgctgccgcttttgaccagtgag336PheSerGlyValLeuAsnTyrSerThrLeuProLeuLeuThrSerGlu100105110gtgcataaacggcttcctaatgtggagttggagctcgttggtcagaag384ValHisLysArgLeuProAsnValGluLeuGluLeuValGlyGlnLys115120125ttgacgagggaagcggtaagtttgctgcgcttgggggcgttggatatt432LeuThrArgGluAlaValSerLeuLeuArgLeuGlyAlaLeuAspIle130135140acgttgatgggtttgcccattgaggatccagagattgagactcggctg480ThrLeuMetGlyLeuProIleGluAspProGluIleGluThrArgLeu145150155160attagtttggaagagttttgcgtggtgttgccgaaggatcatcgtctt528IleSerLeuGluGluPheCysValValLeuProLysAspHisArgLeu165170175gcgggggaaggagtggtggatttggtggatctggctaaagatgggttt576AlaGlyGluGlyValValAspLeuValAspLeuAlaLysAspGlyPhe180185190gtgacgacgccggagtttgcggggtctgtgtttaggaattccaccttt624ValThrThrProGluPheAlaGlySerValPheArgAsnSerThrPhe195200205cagttgtgtgctgaggctggttttgtgccgaggatcagccagcaagtt672GlnLeuCysAlaGluAlaGlyPheValProArgIleSerGlnGlnVal210215220aatgatccttacatggcgctgttgttggcgcgg705AsnAspProTyrMetAlaLeuLeuLeuAlaArg225230235<210>72<211>235<212>PRT<213>谷氨酸棒杆菌<400>72MetGluIleArgTrpLeuGluGlyPheIleAlaValAlaGluGluLeu151015HisPheSerAsnAlaAlaIleArgLeuGlyMetProGlnSerProLeu202530SerGlnLeuIleArgArgLeuGluSerGluLeuGlyGlnLysLeuPhe354045AspArgSerThrArgSerValGluLeuThrAlaAlaGlyArgAlaPhe505560LeuProHisAlaArgGlyIleValAlaSerAlaAlaValAlaArgGlu65707580AlaValAsnAlaAlaGluGlyGluIleValGlyValValArgIleGly859095PheSerGlyValLeuAsnTyrSerThrLeuProLeuLeuThrSerGlu100105110ValHisLysArgLeuProAsnValGluLeuGluLeuValGlyGlnLys115120125LeuThrArgGluAlaValSerLeuLeuArgLeuGlyAlaLeuAspIle130135140ThrLeuMetGlyLeuProIleGluAspProGluIleGluThrArgLeu145150155160IleSerLeuGluGluPheCysValValLeuProLysAspHisArgLeu165170175AlaGlyGluGlyValValAspLeuValAspLeuAlaLysAspGlyPhe180185190ValThrThrProGluPheAlaGlySerValPheArgAsnSerThrPhe195200205GlnLeuCysAlaGluAlaGlyPheValProArgIleSerGlnGlnVal210215220AsnAspProTyrMetAlaLeuLeuLeuAlaArg225230235<210>73<211>849<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(846)<223>RXA02910E<400>73atggcatttccgcttctagcggttgctggcacagttgcccccgttgca48MetAlaPheProLeuLeuAlaValAlaGlyThrValAlaProValAla151015gcaggatgggcaaaagataagtttctcagcaactctcagaataatcag96AlaGlyTrpAlaLysAspLysPheLeuSerAsnSerGlnAsnAsnGln202530caggcccagaatcagcagatgagttttggacaggtcaacaacagcgca144GlnAlaGlnAsnGlnGlnMetSerPheGlyGlnValAsnAsnSerAla354045cagaattctggatcagaaaacagcggtttcatgggtcaatacggcaat192GlnAsnSerGlySerGluAsnSerGlyPheMetGlyGlnTyrGlyAsn505560ctgggtgcaggtttggcaggtgctgctacaggcgcagggcttgcgtat240LeuGlyAlaGlyLeuAlaGlyAlaAlaThrGlyAlaGlyLeuAlaTyr65707580agcgattttgaagatggtcaaagtttgtcctcgaaggcccgcaacatg288SerAspPheGluAspGlyGlnSerLeuSerSerLysAlaArgAsnMet859095gtcggcaaagggctcgctggtgccggagctggtgtgttcaccaagctt336ValGlyLysGlyLeuAlaGlyAlaGlyAlaGlyValPheThrLysLeu100105110gccaatgatgcaattcaggccgagggtggctcgatgaaggctagtgct384AlaAsnAspAlaIleGlnAlaGluGlyGlySerMetLysAlaSerAla115120125tattctgccattgcaggcggcttgggatcgtatctcaaagacggcggt432TyrSerAlaIleAlaGlyGlyLeuGlySerTyrLeuLysAspGlyGly130135140ccgggtgtgattaaatccgcgatggccagcggtgcagccggttttggt480ProGlyValIleLysSerAlaMetAlaSerGlyAlaAlaGlyPheGly145150155160gcggataaagtgcacgataaattagctgagtctggtcatgaggggttg528AlaAspLysValHisAspLysLeuAlaGluSerGlyHisGluGlyLeu165170175gctgattccttgtcgggcgctattcaaggaggtggtcttggttactcc576AlaAspSerLeuSerGlyAlaIleGlnGlyGlyGlyLeuGlyTyrSer180185190acgcttggcggtgtcactggtgctggtattggcggtgcgacgggcggt624ThrLeuGlyGlyValThrGlyAlaGlyIleGlyGlyAlaThrGlyGly195200205ctcgcaggactagcacaaaactactttggtggtggcgatgactacagc672LeuAlaGlyLeuAlaGlnAsnTyrPheGlyGlyGlyAspAspTyrSer210215220aacgctggggcatctgcatcggggtttagtgccaaccaggttaatagt720AsnAlaGlyAlaSerAlaSerGlyPheSerAlaAsnGlnValAsnSer225230235240gagatcagcaccgagattccgcagtttgcgaatcttggtcaaccacag768GluIleSerThrGluIleProGlnPheAlaAsnLeuGlyGlnProGln245250255cgatccgagcttgaacaattagcgctacctcaagaatcacggtctgta816ArgSerGluLeuGluGlnLeuAlaLeuProGlnGluSerArgSerVal260265270gataagagctacgaccaaggctacgaagcgtaa849AspLysSerTyrAspGlnGlyTyrGluAla275280<210>74<211>282<212>PRT<213>谷氨酸棒杆菌<400>74MetAlaPheProLeuLeuAlaValAlaGlyThrValAlaProValAla151015AlaGlyTrpAlaLysAspLysPheLeuSerAsnSerGlnAsnAsnGln202530GlnAlaGlnAsnGlnGlnMetSerPheGlyGlnValAsnAsnSerAla354045GlnAsnSerGlySerGluAsnSerGlyPheMetGlyGlnTyrGlyAsn505560LeuGlyAlaGlyLeuAlaGlyAlaAlaThrGlyAlaGlyLeuAlaTyr65707580SerAspPheGluAspGlyGlnSerLeuSerSerLysAlaArgAsnMet859095ValGlyLysGlyLeuAlaGlyAlaGlyAlaGlyValPheThrLysLeu100105110AlaAsnAspAlaIleGlnAlaGluGlyGlySerMetLysAlaSerAla115120125TyrSerAlaIleAlaGlyGlyLeuGlySerTyrLeuLysAspGlyGly130135140ProGlyValIleLysSerAlaMetAlaSerGlyAlaAlaGlyPheGly145150155160AlaAspLysValHisAspLysLeuAlaGluSerGlyHisGluGlyLeu165170175AlaAspSerLeuSerGlyAlaIleGlnGlyGlyGlyLeuGlyTyrSer180185190ThrLeuGlyGlyValThrGlyAlaGlyIleGlyGlyAlaThrGlyGly195200205LeuAlaGlyLeuAlaGlnAsnTyrPheGlyGlyGlyAspAspTyrSer210215220AsnAlaGlyAlaSerAlaSerGlyPheSerAlaAsnGlnValAsnSer225230235240GluIleSerThrGluIleProGlnPheAlaAsnLeuGlyGlnProGln245250255ArgSerGluLeuGluGlnLeuAlaLeuProGlnGluSerArgSerVal260265270AspLysSerTyrAspGlnGlyTyrGluAla275280<210>75<211>1407<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1407)<223>RXA03083W<220><221>未确定<222>903..903<223>所有出现的n指任何核苷酸<400>75atggcaaagaggatcgtaattatcggcggtggacctgcaggctatgaa48MetAlaLysArgIleValIleIleGlyGlyGlyProAlaGlyTyrGlu151015gccgcactcgcaggcgctaaatacggtgcagaagttaccgttattgaa96AlaAlaLeuAlaGlyAlaLysTyrGlyAlaGluValThrValIleGlu202530gatgtcggagttggcggatccgcagtcaccatggactgtgtaccttca144AspValGlyValGlyGlySerAlaValThrMetAspCysValProSer354045aagtccttcatcgctggtaccggtatcaaaaccgacctccgacgtgct192LysSerPheIleAlaGlyThrGlyIleLysThrAspLeuArgArgAla505560gatgacatgggacttaaccgtgggcttggaaaagcacacctagaaatc240AspAspMetGlyLeuAsnArgGlyLeuGlyLysAlaHisLeuGluIle65707580gatgcactgaacatccgtgtgaaggaccttgcgaaagcacagtccgaa288AspAlaLeuAsnIleArgValLysAspLeuAlaLysAlaGlnSerGlu859095gatatcttgggccagctgcagcgctcagatgtccgcatgattaacggt336AspIleLeuGlyGlnLeuGlnArgSerAspValArgMetIleAsnGly100105110gtgggccgctttgatgattacaacaccaagcaaaccacccactacatt384ValGlyArgPheAspAspTyrAsnThrLysGlnThrThrHisTyrIle115120125aaagtcacccacagcgatggctccgaagaaaccgttgagtgcgatctg432LysValThrHisSerAspGlySerGluGluThrValGluCysAspLeu130135140gtgctggttgcaactggtgcaaccccccgcattcttaaaggtgcagag480ValLeuValAlaThrGlyAlaThrProArgIleLeuLysGlyAlaGlu145150155160ccagacggcgagcgcatcttgacctggcgtcaggtctacgacattgaa528ProAspGlyGluArgIleLeuThrTrpArgGlnValTyrAspIleGlu165170175gaactccccacccaccttatcgtggttggttccggtgtgaccggtgcg576GluLeuProThrHisLeuIleValValGlySerGlyValThrGlyAla180185190gaatttgtctctgcgtttgctgaactcggcgtcaaagtcaccatggtg624GluPheValSerAlaPheAlaGluLeuGlyValLysValThrMetVal195200205gcatcccgtgaccgcattttgcctcacgatgacgcagatgccgcagac672AlaSerArgAspArgIleLeuProHisAspAspAlaAspAlaAlaAsp210215220gtgctggaaaccgttctggctgagcgcggagtatccctggaaaagcat720ValLeuGluThrValLeuAlaGluArgGlyValSerLeuGluLysHis225230235240gcccgcgtggagtctgtcacccgcaccgaagacggtggcgtgtgtgtt768AlaArgValGluSerValThrArgThrGluAspGlyGlyValCysVal245250255cgcactgctgacggacgagaaatctacggttctcacgcgttgatgact816ArgThrAlaAspGlyArgGluIleTyrGlySerHisAlaLeuMetThr260265270gttggttccattccaaacacggcagatcttggcctggagaacatcggt864ValGlySerIleProAsnThrAlaAspLeuGlyLeuGluAsnIleGly275280285gttgagctggcaccatccggccatatcaaggttgaccgngtctcccgc912ValGluLeuAlaProSerGlyHisIleLysValAspArgValSerArg290295300accaacatccccggtgtgtacgcagcaggtgactgtactgacctattc960ThrAsnIleProGlyValTyrAlaAlaGlyAspCysThrAspLeuPhe305310315320ccactggcgtccgttgcagcgatgcagggccgtatcgccatgtatcac1008ProLeuAlaSerValAlaAlaMetGlnGlyArgIleAlaMetTyrHis325330335gcactcggtgaaggcgtgagccccatccgtttgaagactgttgccacc1056AlaLeuGlyGluGlyValSerProIleArgLeuLysThrValAlaThr340345350gcagtgtttacccgcccagagatcgcagcagtaggtatcacccatgca1104AlaValPheThrArgProGluIleAlaAlaValGlyIleThrHisAla355360365caagttgattccggcgaagtgtctgctcgcgtgattgtgcttcctttg1152GlnValAspSerGlyGluValSerAlaArgValIleValLeuProLeu370375380gctactaacccacgcgccaagatgcgttccctgcgccacggttttgtg1200AlaThrAsnProArgAlaLysMetArgSerLeuArgHisGlyPheVal385390395400aagctgttctgccgccgtaactctggcctgatcatcggtggtgtcgtg1248LysLeuPheCysArgArgAsnSerGlyLeuIleIleGlyGlyValVal405410415gtggcaccgaccgcgtctgagctgatcctaccgatcgctgtggcagtg1296ValAlaProThrAlaSerGluLeuIleLeuProIleAlaValAlaVal420425430accaaccgtctgacagttgctgatctggctgataccttcgcggtgtac1344ThrAsnArgLeuThrValAlaAspLeuAlaAspThrPheAlaValTyr435440445ccatcattgtcaggttcgattactgaagcagcacgtcagctggttcaa1392ProSerLeuSerGlySerIleThrGluAlaAlaArgGlnLeuValGln450455460catgatgatctaggc1407HisAspAspLeuGly465<210>76<211>469<212>PRT<213>谷氨酸棒杆菌<400>76MetAlaLysArgIleValIleIleGlyGlyGlyProAlaGlyTyrGlu151015AlaAlaLeuAlaGlyAlaLysTyrGlyAlaGluValThrValIleGlu202530AspValGlyValGlyGlySerAlaValThrMetAspCysValProSer354045LysSerPheIleAlaGlyThrGlyIleLysThrAspLeuArgArgAla505560AspAspMetGlyLeuAsnArgGlyLeuGlyLysAlaHisLeuGluIle65707580AspAlaLeuAsnIleArgValLysAspLeuAlaLysAlaGlnSerGlu859095AspIleLeuGlyGlnLeuGlnArgSerAspValArgMetIleAsnGly100105110ValGlyArgPheAspAspTyrAsnThrLysGlnThrThrHisTyrIle115120125LysValThrHisSerAspGlySerGluGluThrValGluCysAspLeu130135140ValLeuValAlaThrGlyAlaThrProArgIleLeuLysGlyAlaGlu145150155160ProAspGlyGluArgIleLeuThrTrpArgGlnValTyrAspIleGlu165170175GluLeuProThrHisLeuIleValValGlySerGlyValThrGlyAla180185190GluPheValSerAlaPheAlaGluLeuGlyValLysValThrMetVal195200205AlaSerArgAspArgIleLeuProHisAspAspAlaAspAlaAlaAsp210215220ValLeuGluThrValLeuAlaGluArgGlyValSerLeuGluLysHis225230235240AlaArgValGluSerValThrArgThrGluAspGlyGlyValCysVal245250255ArgThrAlaAspGlyArgGluIleTyrGlySerHisAlaLeuMetThr260265270ValGlySerIleProAsnThrAlaAspLeuGlyLeuGluAsnIleGly275280285ValGluLeuAlaProSerGlyHisIleLysValAspArgValSerArg290295300ThrAsnIleProGlyValTyrAlaAlaGlyAspCysThrAspLeuPhe305310315320ProLeuAlaSerValAlaAlaMetGlnGlyArgIleAlaMetTyrHis325330335AlaLeuGlyGluGlyValSerProIleArgLeuLysThrValAlaThr340345350AlaValPheThrArgProGluIleAlaAlaValGlyIleThrHisAla355360365GlnValAspSerGlyGluValSerAlaArgValIleValLeuProLeu370375380AlaThrAsnProArgAlaLysMetArgSerLeuArgHisGlyPheVal385390395400LysLeuPheCysArgArgAsnSerGlyLeuIleIleGlyGlyValVal405410415ValAlaProThrAlaSerGluLeuIleLeuProIleAlaValAlaVal420425430ThrAsnArgLeuThrValAlaAspLeuAlaAspThrPheAlaValTyr435440445ProSerLeuSerGlySerIleThrGluAlaAlaArgGlnLeuValGln450455460HisAspAspLeuGly465<210>77<211>792<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(792)<223>RXA03802K<400>77atggtcagccaaacggaaagacagcatgcaattgcttctttactggca48MetValSerGlnThrGluArgGlnHisAlaIleAlaSerLeuLeuAla151015ccaactggtgcggtgtctgtaggagatttagccgagcatttccatgtg96ProThrGlyAlaValSerValGlyAspLeuAlaGluHisPheHisVal202530acaaccgaaacagtgcgacgtgatcttcggatcatggagtcactgggt144ThrThrGluThrValArgArgAspLeuArgIleMetGluSerLeuGly354045ttgttgcaacgagttcacggtggcgccattagcccagagcccatgggt192LeuLeuGlnArgValHisGlyGlyAlaIleSerProGluProMetGly505560acgtctccccctcggctgaaacctgccttgggtaaaggaatgccaccg240ThrSerProProArgLeuLysProAlaLeuGlyLysGlyMetProPro65707580gaaccccgtgttttagaacttgcagaaactgcagtttccctcatcaca288GluProArgValLeuGluLeuAlaGluThrAlaValSerLeuIleThr859095cctctagcacgcagcattttcctggattcaggtttagcgtgcacggcg336ProLeuAlaArgSerIlePheLeuAspSerGlyLeuAlaCysThrAla100105110attgccacggtgttgggggatcctccagaagatgccaggtggactgtt384IleAlaThrValLeuGlyAspProProGluAspAlaArgTrpThrVal115120125gttacaagttcccccggcgctgtgattgccttgtccgcgacagatgcc432ValThrSerSerProGlyAlaValIleAlaLeuSerAlaThrAspAla130135140acctccacggtggtgctgcacgggcaggttcacggtaattgttcttca480ThrSerThrValValLeuHisGlyGlnValHisGlyAsnCysSerSer145150155160atcattgggtccacggcagtagacatgatttcgcagttgcgcgctgat528IleIleGlySerThrAlaValAspMetIleSerGlnLeuArgAlaAsp165170175atcgccttcgtggaggttgatgcgattcaatccgatacaagtctgtgc576IleAlaPheValGluValAspAlaIleGlnSerAspThrSerLeuCys180185190acgtttttcccggagacgattcccatcaagcaagccatgatcaaaaac624ThrPhePheProGluThrIleProIleLysGlnAlaMetIleLysAsn195200205gcggctttcacagttgctgttctcagcccgagatctccccaagatcaa672AlaAlaPheThrValAlaValLeuSerProArgSerProGlnAspGln210215220gaacttcaacttttgaagcaccctttttccaccttggctgattttgat720GluLeuGlnLeuLeuLysHisProPheSerThrLeuAlaAspPheAsp225230235240gcccttgttaccgatgaccacacgctagattttccagttttgcccgac768AlaLeuValThrAspAspHisThrLeuAspPheProValLeuProAsp245250255cacaactttcaggtggtaacccca792HisAsnPheGlnValValThrPro260<210>78<211>264<212>PRT<213>谷氨酸棒杆菌<400>78MetValSerGlnThrGluArgGlnHisAlaIleAlaSerLeuLeuAla151015ProThrGlyAlaValSerValGlyAspLeuAlaGluHisPheHisVal202530ThrThrGluThrValArgArgAspLeuArgIleMetGluSerLeuGly354045LeuLeuGlnArgValHisGlyGlyAlaIleSerProGluProMetGly505560ThrSerProProArgLeuLysProAlaLeuGlyLysGlyMetProPro65707580GluProArgValLeuGluLeuAlaGluThrAlaValSerLeuIleThr859095ProLeuAlaArgSerIlePheLeuAspSerGlyLeuAlaCysThrAla100105110IleAlaThrValLeuGlyAspProProGluAspAlaArgTrpThrVal115120125ValThrSerSerProGlyAlaValIleAlaLeuSerAlaThrAspAla130135140ThrSerThrValValLeuHisGlyGlnValHisGlyAsnCysSerSer145150155160IleIleGlySerThrAlaValAspMetIleSerGlnLeuArgAlaAsp165170175IleAlaPheValGluValAspAlaIleGlnSerAspThrSerLeuCys180185190ThrPhePheProGluThrIleProIleLysGlnAlaMetIleLysAsn195200205AlaAlaPheThrValAlaValLeuSerProArgSerProGlnAspGln210215220GluLeuGlnLeuLeuLysHisProPheSerThrLeuAlaAspPheAsp225230235240AlaLeuValThrAspAspHisThrLeuAspPheProValLeuProAsp245250255HisAsnPheGlnValValThrPro260<210>79<211>3420<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3420)<223>RXA04031K<400>79gtgtcgactcacacatcttcaacgcttccagcattcaaaaagatcttg48MetSerThrHisThrSerSerThrLeuProAlaPheLysLysIleLeu151015gtagcaaaccgcggcgaaatcgcggtccgtgctttccgtgcagcactc96ValAlaAsnArgGlyGluIleAlaValArgAlaPheArgAlaAlaLeu202530gaaaccggtgcagccacggtagctatttacccccgtgaagatcgggga144GluThrGlyAlaAlaThrValAlaIleTyrProArgGluAspArgGly354045tcattccaccgctcttttgcttctgaagctgtccgcattggtaccgaa192SerPheHisArgSerPheAlaSerGluAlaValArgIleGlyThrGlu505560ggctcaccagtcaaggcgtacctggacatcgatgaaattatcggtgca240GlySerProValLysAlaTyrLeuAspIleAspGluIleIleGlyAla65707580gctaaaaaagttaaagcagatgccatttacccgggatacggcttcctg288AlaLysLysValLysAlaAspAlaIleTyrProGlyTyrGlyPheLeu859095tctgaaaatgcccagcttgcccgcgagtgtgcggaaaacggcattact336SerGluAsnAlaGlnLeuAlaArgGluCysAlaGluAsnGlyIleThr100105110tttattggcccaaccccagaggttcttgatctcaccggtgataagtct384PheIleGlyProThrProGluValLeuAspLeuThrGlyAspLysSer115120125cgcgcggtaaccgccgcgaagaaggctggtctgccagttttggcggaa432ArgAlaValThrAlaAlaLysLysAlaGlyLeuProValLeuAlaGlu130135140tccaccccgagcaaaaacatcgatgagatcgttaaaagcgctgaaggc480SerThrProSerLysAsnIleAspGluIleValLysSerAlaGluGly145150155160cagacttaccccatctttgtgaaggcagttgccggtggtggcggacgc528GlnThrTyrProIlePheValLysAlaValAlaGlyGlyGlyGlyArg165170175ggtatgcgttttgttgcttcacctgatgagcttcgcaaattagcaaca576GlyMetArgPheValAlaSerProAspGluLeuArgLysLeuAlaThr180185190gaagcatctcgtgaagctgaagcggctttcggcgatggcgcggtatat624GluAlaSerArgGluAlaGluAlaAlaPheGlyAspGlyAlaValTyr195200205gtcgaacgtgctgtgattaaccctcagcatattgaagtgcagatcctt672ValGluArgAlaValIleAsnProGlnHisIleGluValGlnIleLeu210215220ggcgatcacactggagaagttgtacacctttatgaacgtgactgctca720GlyAspHisThrGlyGluValValHisLeuTyrGluArgAspCysSer225230235240ctgcagcgtcgtcaccaaaaagttgtcgaaattgcgccagcacagcat768LeuGlnArgArgHisGlnLysValValGluIleAlaProAlaGlnHis245250255ttggatccagaactgcgtgatcgcatttgtgcggatgcagtaaagttc816LeuAspProGluLeuArgAspArgIleCysAlaAspAlaValLysPhe260265270tgccgctccattggttaccagggcgcgggaaccgtggaattcttggtc864CysArgSerIleGlyTyrGlnGlyAlaGlyThrValGluPheLeuVal275280285gatgaaaagggcaaccacgtcttcatcgaaatgaacccacgtatccag912AspGluLysGlyAsnHisValPheIleGluMetAsnProArgIleGln290295300gttgagcacaccgtgactgaagaagtcaccgaggtggacctggtgaag960ValGluHisThrValThrGluGluValThrGluValAspLeuValLys305310315320gcgcagatgcgcttggctgctggtgcaaccttgaaggaattgggtctg1008AlaGlnMetArgLeuAlaAlaGlyAlaThrLeuLysGluLeuGlyLeu325330335acccaagataagatcaagacccacggtgcagcactgcagtgccgcatc1056ThrGlnAspLysIleLysThrHisGlyAlaAlaLeuGlnCysArgIle340345350accacggaagatccaaacaacggcttccgcccagataccggaactatc1104ThrThrGluAspProAsnAsnGlyPheArgProAspThrGlyThrlle355360365accgcgtaccgctcaccaggcggagctggcgttcgtcttgacggtgca1152ThrAlaTyrArgSerProGlyGlyAlaGlyValArgLeuAspGlyAla370375380gctcagctcggtggcgaaatcaccgcacactttgactccatgctggtg1200AlaGlnLeuGlyGlyGluIleThrAlaHisPheAspSerMetLeuVal385390395400aaaatgacctgccgtggttccgactttgaaactgctgttgctcgtgca1248LysMetThrCysArgGlySerAspPheGluThrAlaValAlaArgAla405410415cagcgcgcgttggctgagttcaccgtgtctggtgttgcaaccaacatt1296GlnArgAlaLeuAlaGluPheThrValSerGlyValAlaThrAsnIle420425430ggtttcttgcgtgcgttgctgcgggaagaggacttcacttccaagcgc1344GlyPheLeuArgAlaLeuLeuArgGluGluAspPheThrSerLysArg435440445atcgccaccggattcattgccgatcacccgcacctccttcaggctcca1392IleAlaThrGlyPheIleAlaAspHisProHisLeuLeuGlnAlaPro450455460cctgctgatgatgagcagggacgcatcctggattacttggcagatgtc1440ProAlaAspAspGluGlnGlyArgIleLeuAspTyrLeuAlaAspVal465470475480accgtgaacaagcctcatggtgtgcgtccaaaggatgttgcagctcct1488ThrValAsnLysProHisGlyValArgProLysAspValAlaAlaPro485490495atcgataagctgcctaacatcaaggatctgccactgccacgcggttcc1536IleAspLysLeuProAsnIleLysAspLeuProLeuProArgGlySer500505510cgtgaccgcctgaagcagcttggcccagccgcgtttgctcgtgatctc1584ArgAspArgLeuLysG1nLeuG1yProAlaAlaPheAlaArgAspLeu515520525cgtgagcaggacgcactggcagttactgataccaccttccgcgatgca1632ArgGluGlnAspAlaLeuAlaValThrAspThrThrPheArgAspAla530535540caccagtctttgcttgcgacccgagtccgctcattcgcactgaagcct1680HisGlnSerLeuLeuAlaThrArgValArgSerPheAlaLeuLysPro545550555560gcggcagaggccgtcgcaaagctgactcctgagcttttgtccgtggag1728AlaAlaGluAlaValAlaLysLeuThrProGluLeuLeuSerValGlu565570575gcctggggcggcgcgacctacgatgtggcgatgcgtttcctctttgag1776AlaTrpGlyGlyAlaThrTyrAspValAlaMetArgPheLeuPheGlu580585590gatccgtgggacaggctcgacgagctgcgcgaggcgatgccgaatgta1824AspProTrpAspArgLeuAspGluLeuArgGluAlaMetProAsnVal595600605aacattcagatgctgcttcgcggccgcaacaccgtgggatacaccccg1872AsnIleGlnMetLeuLeuArgGlyArgAsnThrValGlyTyrThrPro610615620tacccagactccgtctgccgcgcgtttgttaaggaagctgccagctcc1920TyrProAspSerValCysArgAlaPheValLysGluAlaAlaSerSer625630635640ggcgtggacatcttccgcatcttcgacgcgcttaacgacgtctcccag1968GlyValAspIlePheArgIlePheAspAlaLeuAsnAspValSerGln645650655atgcgtccagcaatcgacgcagtcctggagaccaacaccgcggtagcc2016MetArgProAlaIleAspAlaValLeuGluThrAsnThrAlaValAla660665670gaggtggctatggcttattctggtgatctctctgatccaaatgaaaag2064GluValAlaMetAlaTyrSerGlyAspLeuSerAspProAsnGluLys675680685ctctacaccctggattactacctaaagatggcagaggagatcgtcaag2112LeuTyrThrLeuAspTyrTyrLeuLysMetAlaGluGluIleValLys690695700tctggcgctcacatcttggccattaaggatatggctggtctgcttcgc2160SerGlyAlaHisIleLeuAlaIleLysAspMetAlaGlyLeuLeuArg705710715720ccagctgcggtaaccaagctggtcaccgcactgcgccgtgaattcgat2208ProAlaAlaValThrLysLeuValThrAlaLeuArgArgGluPheAsp725730735ctgccagtgcacgtgcacacccacgacactgcgggtggccagctggca2256LeuProValHisValHisThrHisAspThrAlaGlyGlyGlnLeuAla740745750acctactttgctgcagctcaagctggtgcagatgctgttgacggtgct2304ThrTyrPheAlaAlaAlaGlnAlaGlyAlaAspAlaValAspGlyAla755760765tccgcaccactgtctggcaccacctcccagccatccctgtctgccatt2352SerAlaProLeuSerGlyThrThrSerGlnProSerLeuSerAlaIle770775780gttgctgcattcgcgcacacccgtcgcgataccggtttgagcctcgag2400ValAlaAlaPheAlaHisThrArgArgAspThrGlyLeuSerLeuGlu785790795800gctgtttctgacctcgagccgtactgggaagcagtgcgcggactgtac2448AlaValSerAspLeuGluProTyrTrpGluAlaValArgGlyLeuTyr805810815ctgccatttgagtctggaaccccaggcccaaccggtcgcgtctaccgc2496LeuProPheGluSerGlyThrProGlyProThrGlyArgValTyrArg820825830cacgaaatcccaggcggacagttgtccaacctgcgtgcacaggccacc2544HisGluIleProGlyGlyGlnLeuSerAsnLeuArgAlaGlnAlaThr835840845gcactgggccttgcggatcgtttcgaactcatcgaagacaactacgca2592AlaLeuGlyLeuAlaAspArgPheGluLeuIleGluAspAsnTyrAla850855860gccgttaatgagatgctgggacgcccaaccaaggtcaccccatcctcc2640AlaValAsnGluMetLeuGlyArgProThrLysValThrProSerSer865870875880aaggttgttggcgacctcgcactccacctcgttggtgcgggtgtggat2688LysValValGlyAspLeuAlaLeuHisLeuValGlyAlaGlyValAsp885890895ccagcagactttgctgccgatccacaaaagtacgacatcccagactct2736ProAlaAspPheAlaAlaAspProGlnLysTyrAspIleProAspSer900905910gtcatcgcgttcctgcgcggcgagcttggtaaccctccaggtggctgg2784ValIleAlaPheLeuArgGlyGluLeuGlyAsnProProGlyGlyTrp915920925ccagagccactgcgcacccgcgcactggaaggccgctccgaaggcaag2832ProGluProLeuArgThrArgAlaLeuGluGlyArgSerGluGlyLys930935940gcacctctgacggaagttcctgaggaagagcaggcgcacctcgacgct2880AlaProLeuThrGluValProGluGluGluGlnAlaHisLeuAspAla945950955960gatgattccaaggaacgtcgcaatagcctcaaccgcctgctgttcccg2928AspAspSerLysGluArgArgAsnSerLeuAsnArgLeuLeuPhePro965970975aagccaaccgaagagttcctcgagcaccgtcgccgcttcggcaacacc2976LysProThrGluGluPheLeuGluHisArgArgArgPheGlyAsnThr980985990tctgcgctggatgatcgtgaattcttctacggcctggtcgaaggccgc3024SerAlaLeuAspAspArgGluPhePheTyrGlyLeuValGluGlyArg99510001005gagactttgatccgcctgccagatgtgcgcaccccactgcttgttcgc3072GluThrLeuIleArgLeuProAspValArgThrProLeuLeuValArg101010151020ctggatgcgatctctgagccagacgataagggtatgcgcaatgttgtg3120LeuAspAlaIleSerGluProAspAspLysGlyMetArgAsnValVal1025103010351040gccaacgtcaacggccagatccgcccaatgcgtgtgcgtgaccgctcc3168AlaAsnValAsnGlyGlnIleArgProMetArgValArgAspArgSer104510501055gttgagtctgtcaccgcaaccgcagaaaaggcagattcctccaacaag3216ValGluSerValThrAlaThrAlaGluLysAlaAspSerSerAsnLys106010651070ggccatgttgctgcaccattcgctggtgttgtcaccgtgactgttgct3264GlyHisValAlaAlaProPheAlaGlyValValThrValThrValAla107510801085gaaggtgatgaggtcaaggctggagatgcagtcgcaatcatcgaggct3312GluGlyAspGluValLysAlaGlyAspAlaValAlaIleIleGluAla109010951100atgaagatggaagcaacaatcactgcttctgttgacggcaaaatcgat3360MetLysMetGluAlaThrIleThrAlaSerValAspGlyLysIleAsp1105111011151120cgcgttgtggttcctgctgcaacgaaggtggaaggtggcgacttgatc3408ArgValValValProAlaAlaThrLysValGluGlyGlyAspLeuIle112511301135gtcgtcgtttcc3420ValValValSer1140<210>80<211>1140<212>PRT<213>谷氨酸棒杆菌<400>80MetSerThrHisThrSerSerThrLeuProAlaPheLysLysIleLeu151015ValAlaAsnArgGlyGluIleAlaValArgAlaPheArgAlaAlaLeu202530GluThrGlyAlaAlaThrValAlaIleTyrProArgGluAspArgGly354045SerPheHisArgSerPheAlaSerGluAlaValArgIleGlyThrGlu505560GlySerProValLysAlaTyrLeuAspIleAspGluIleIleGlyAla65707580AlaLysLysValLysAlaAspAlaIleTyrProGlyTyrGlyPheLeu859095SerGluAsnAlaGlnLeuAlaArgGluCysAlaGluAsnGlyIleThr100105110PheIleGlyProThrProGluValLeuAspLeuThrGlyAspLysSer115120125ArgAlaValThrAlaAlaLysLysAlaGlyLeuProValLeuAlaGlu130135140SerThrProSerLysAsnIleAspGluIleValLysSerAlaGluGly145150155160GlnThrTyrProIlePheValLysAlaValAlaGlyGlyGlyGlyArg165170175GlyMetArgPheValAlaSerProAspGluLeuArgLysLeuAlaThr180185190GluAlaSerArgGluAlaGluAlaAlaPheGlyAspGlyAlaValTyr195200205ValGluArgAlaValIleAsnProGlnHisIleGluValGlnIleLeu210215220GlyAspHisThrGlyGluValValHisLeuTyrGluArgAspCysSer225230235240LeuGlnArgArgHisGlnLysValValGluIleAlaProAlaGlnHis245250255LeuAspProGluLeuArgAspArgIleCysAlaAspAlaValLysPhe260265270CysArgSerIleGlyTyrGlnGlyAlaGlyThrValGluPheLeuVal275280285AspGluLysGlyAsnHisValPheIleGluMetAsnProArgIleGln290295300ValGluHisThrValThrGluGluValThrGluValAspLeuValLys305310315320AlaGlnMetArgLeuAlaAlaGlyAlaThrLeuLysGluLeuGlyLeu325330335ThrGlnAspLysIleLysThrHisGlyAlaAlaLeuGlnCysArgIle340345350ThrThrGluAspProAsnAsnGlyPheArgProAspThrGlyThrIle355360365ThrAlaTyrArgSerProGlyGlyAlaGlyValArgLeuAspGlyAla370375380AlaGlnLeuGlyGlyGluIleThrAlaHisPheAspSerMetLeuVal385390395400LysMetThrCysArgGlySerAspPheGluThrAlaValAlaArgAla405410415GlnArgAlaLeuAlaGluPheThrValSerGlyValAlaThrAsnIle420425430GlyPheLeuArgAlaLeuLeuArgGluGluAspPheThrSerLysArg435440445IleAlaThrGlyPheIleAlaAspHisProHisLeuLeuGlnAlaPro450455460ProAlaAspAspGluGlnGlyArgIleLeuAspTyrLeuAlaAspVal465470475480ThrValAsnLysProHisGlyValArgProLysAspValAlaAlaPro485490495IleAspLysLeuProAsnIleLysAspLeuProLeuProArgGlySer500505510ArgAspArgLeuLysGlnLeuGlyProAlaAlaPheAlaArgAspLeu515520525ArgGluGlnAspAlaLeuAlaValThrAspThrThrPheArgAspAla530535540HisGlnSerLeuLeuAlaThrArgValArgSerPheAlaLeuLysPro545550555560AlaAlaGluAlaValAlaLysLeuThrProGluLeuLeuSerValGlu565570575AlaTrpGlyGlyAlaThrTyrAspValAlaMetArgPheLeuPheGlu580585590AspProTrpAspArgLeuAspGluLeuArgGluAlaMetProAsnVal595600605AsnIleGlnMetLeuLeuArgGlyArgAsnThrValGlyTyrThrPro610615620TyrProAspSerValCysArgAlaPheValLysGluAlaAlaSerSer625630635640GlyValAspIlePheArgIlePheAspAlaLeuAsnAspValSerGln645650655MetArgProAlaIleAspAlaValLeuGluThrAsnThrAlaValAla660665670GluValAlaMetAlaTyrSerGlyAspLeuSerAspProAsnGluLys675680685LeuTyrThrLeuAspTyrTyrLeuLysMetAlaGluGluIleValLys690695700SerGlyAlaHisIleLeuAlaIleLysAspMetAlaGlyLeuLeuArg705710715720ProAlaAlaValThrLysLeuValThrAlaLeuArgArgGluPheAsp725730735LeuProValHisValHisThrHisAspThrAlaGlyGlyGlnLeuAla740745750ThrTyrPheAlaAlaAlaGlnAlaGlyAlaAspAlaValAspGlyAla755760765SerAlaProLeuSerGlyThrThrSerGlnProSerLeuSerAlaIle770775780ValAlaAlaPheAlaHisThrArgArgAspThrGlyLeuSerLeuGlu785790795800AlaValSerAspLeuGluProTyrTrpGluAlaValArgGlyLeuTyr805810815LeuProPheGluSerGlyThrProGlyProThrGlyArgValTyrArg820825830HisGluIleProGlyGlyGlnLeuSerAsnLeuArgAlaGlnAlaThr835840845AlaLeuGlyLeuAlaAspArgPheGluLeuIleGluAspAsnTyrAla850855860AlaValAsnGluMetLeuGlyArgProThrLysValThrProSerSer865870875880LysValValGlyAspLeuAlaLeuHisLeuValGlyAlaGlyValAsp885890895ProAlaAspPheAlaAlaAspProGlnLysTyrAspIleProAspSer900905910ValIleAlaPheLeuArgGlyGluLeuGlyAsnProProGlyGlyTrp915920925ProGluProLeuArgThrArgAlaLeuGluGlyArgSerGluGlyLys930935940AlaProLeuThrGluValProGluGluGluGlnAlaHisLeuAspAla945950955960AspAspSerLysGluArgArgAsnSerLeuAsnArgLeuLeuPhePro965970975LysProThrGluGluPheLeuGluHisArgArgArgPheGlyAsnThr980985990SerAlaLeuAspAspArgGluPhePheTyrGlyLeuValGluGlyArg99510001005GluThrLeuIleArgLeuProAspValArgThrProLeuLeuValArg101010151020LeuAspAlaIleSerGluProAspAspLysGlyMetArgAsnValVal1025103010351040AlaAsnValAsnGlyGlnIleArgProMetArgValArgAspArgSer104510501055ValGluSerValThrAlaThrAlaGluLysAlaAspSerSerAsnLys106010651070GlyHisValAlaAlaProPheAlaGlyValValThrValThrValAla107510801085GluGlyAspGluValLysAlaGlyAspAlaValAlaIleIleGluAla109010951100MetLysMetGluAlaThrIleThrAlaSerValAspGlyLysIleAsp1105111011151120ArgValValValProAlaAlaThrLysValGluGlyGlyAspLeuIle112511301135ValValValSer1140<210>81<211>366<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(366)<223>RXA04073K<400>81gtgtttggcgatgtgatcagactaagtgatcaccgtcaccagcaaaag48MetPheGlyAspValIleArgLeuSerAspHisArgHisGlnGlnLys151015gggtttgcgaactttactaagtcattacccccgcctaaccccgacttt96GlyPheAlaAsnPheThrLysSerLeuProProProAsnProAspPhe202530tatctaggtcacaccttcgaaacctacggaacgttgcggtgcctgcat144TyrLeuGlyHisThrPheGluThrTyrGlyThrLeuArgCysLeuHis354045tttcccatttcagagcatttgcccagtacatccgtactagcaactccc192PheProIleSerGluHisLeuProSerThrSerValLeuAlaThrPro505560ccgcccactttttctgcgaagccagaactttgcaaacttcacaacagg240ProProThrPheSerAlaLysProGluLeuCysLysLeuHisAsnArg65707580ggtgaccacccccgcacaaaacttaaaaacccaaaccgattgacgcac288GlyAspHisProArgThrLysLeuLysAsnProAsnArgLeuThrHis859095caatgcccgatggagcaatgtgtgaaccacgccaccacgcaaaccgat336GlnCysProMetGluGlnCysValAsnHisAlaThrThrGlnThrAsp100105110gcacatcacgtcgaaacagtgacagtgcat366AlaHisHisValGluThrValThrValHis115120<210>82<211>122<212>PRT<213>谷氨酸棒杆菌<400>82MetPheGlyAspValIleArgLeuSerAspHisArgHisGlnGlnLys151015GlyPheAlaAsnPheThrLysSerLeuProProProAsnProAspPhe202530TyrLeuGlyHisThrPheGluThrTyrGlyThrLeuArgCysLeuHis354045PheProIleSerGluHisLeuProSerThrSerValLeuAlaThrPro505560ProProThrPheSerAlaLysProGluLeuCysLysLeuHisAsnArg65707580GlyAspHisProArgThrLysLeuLysAsnProAsnArgLeuThrHis859095GlnCysProMetGluGlnCysValAsnHisAlaThrThrGlnThrAsp100105110AlaHisHisValGluThrValThrValHis115120<210>83<211>2829<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2829)<223>RXA04174K<400>83ttggagctcactgtgactgaaagcaagaactccttcaatgctaagagc48MetGluLeuThrValThrGluSerLysAsnSerPheAsnAlaLysSer151015acccttgaagttggcgacaagtcctatgactacttcgccctctctgca96ThrLeuGluValGlyAspLysSerTyrAspTyrPheAlaLeuSerAla202530gtgcctggcatggagaagctgccgtactccctcaaggttctcggagag144ValProGlyMetGluLysLeuProTyrSerLeuLysValLeuGlyGlu354045aaccttcttcgtaccgaagacggcgcaaacatcaccaacgagcacatt192AsnLeuLeuArgThrGluAspGlyAlaAsnIleThrAsnGluHisIle505560gaggctatcgccaactgggatgcatcttccgatccaagcatcgaaatc240GluAlaIleAlaAsnTrpAspAlaSerSerAspProSerIleGluIle65707580cagttcaccccagcccgtgttctcatgcaggacttcaccggtgtccct288GlnPheThrProAlaArgValLeuMetGlnAspPheThrGlyValPro859095tgtgtagttgacctcgcaaccatgcgtgaggcagttgctgcactcggt336CysValValAspLeuAlaThrMetArgGluAlaValAlaAlaLeuGly100105110ggcgaccctaacgacgtcaacccactgaacccagccgagatggtcatt384GlyAspProAsnAspValAsnProLeuAsnProAlaGluMetValIle115120125gaccactccgtcatcgtggaggctttcggccgcccagatgcactggct432AspHisSerValIleValGluAlaPheGlyArgProAspAlaLeuAla130135140aagaacgttgagatcgagtacgagcgcaacgaggagcgttaccagttc480LysAsnValGluIleGluTyrGluArgAsnGluGluArgTyrGlnPhe145150155160ctgcgttggggttccgagtccttctccaacttccgcgttgttcctcca528LeuArgTrpGlySerGluSerPheSerAsnPheArgValValProPro165170175ggaaccggtatcgtccaccaggtcaacattgagtacttggctcgcgtc576GlyThrGlyIleValHisGlnValAsnIleGluTyrLeuAlaArgVal180185190gtcttcgacaacgagggccttgcatacccagatacctgcatcggtacc624ValPheAspAsnGluGlyLeuAlaTyrProAspThrCysIleGlyThr195200205gactcccacaccaccatggaaaacggcctgggcatcctgggctggggc672AspSerHisThrThrMetGluAsnGlyLeuGlyIleLeuGlyTrpGly210215220gttggtggcattgaggctgaagcagcaatgctcggccagccagtgtcc720ValGlyGlyIleGluAlaGluAlaAlaMetLeuGlyGlnProValSer225230235240atgctgatccctcgcgttgttggcttcaagttgaccggcgagatccca768MetLeuIleProArgValValGlyPheLysLeuThrGlyGluIlePro245250255gtaggcgttaccgcaactgacgttgtgctgaccatcaccgaaatgctg816ValGlyValThrAlaThrAspValValLeuThrIleThrGluMetLeu260265270cgcgaccacggcgtcgtccagaagttcgttgagttctacggctccggt864ArgAspHisGlyValValGlnLysPheValGluPheTyrGlySerGly275280285gttaaggctgttccactggctaaccgtgcaaccatcggcaacatgtcc912ValLysAlaValProLeuAlaAsnArgAlaThrIleGlyAsnMetSer290295300ccagagttcggctccacctgtgcgatgttcccaatcgacgaggagacc960ProGluPheGlySerThrCysAlaMetPheProIleAspGluGluThr305310315320accaagtacctgcgcctcaccggccgcccagaagagcaggttgcactg1008ThrLysTyrLeuArgLeuThrGlyArgProGluGluGlnValAlaLeu325330335gtcgaggcttacgccaaggcgcagggcatgtggctcgacgaggacacc1056ValGluAlaTyrAlaLysAlaGlnGlyMetTrpLeuAspGluAspThr340345350gttgaagctgagtactccgagtacctcgagctggacctgtccaccgtt1104ValGluAlaGluTyrSerGluTyrLeuGluLeuAspLeuSerThrVal355360365gttccttccatcgctggccctaagcgcccacaggaccgcatccttctc1152ValProSerIleAlaGlyProLysArgProGlnAspArgIleLeuLeu370375380tccgaggcaaaggagcagttccgtaaggatctgccaacctacaccgac1200SerGluAlaLysGluGlnPheArgLysAspLeuProThrTyrThrAsp385390395400gacgctgtttccgtagacacctccatccctgcaacccgcatggttaac1248AspAlaValSerValAspThrSerIleProAlaThrArgMetValAsn405410415gaaggtggcggacagcctgaaggcggcgtcgaagctgacaactacaac1296GluGlyGlyGlyGlnProGluGlyGlyValGluAlaAspAsnTyrAsn420425430gcttcctgggctggctccggcgagtccttggctactggcgcagaagga1344AlaSerTrpAlaGlySerGlyGluSerLeuAlaThrGlyAlaGluGly435440445cgtccttccaagccagtcaccgttgcatccccacagggtggcgagtac1392ArgProSerLysProValThrValAlaSerProGlnGlyGlyGluTyr450455460accatcgaccacggcatggttgcaattgcatccatcacctcttgcacc1440ThrIleAspHisGlyMetValAlaIleAlaSerIleThrSerCysThr465470475480aacacctctaacccatccgtgatgatcggcgctggcctgatcgcacgt1488AsnThrSerAsnProSerValMetIleGlyAlaGlyLeuIleAlaArg485490495aaggcagcagaaaagggcctcaagtccaagccttgggttaagaccatc1536LysAlaAlaGluLysGlyLeuLysSerLysProTrpValLysThrIle500505510tgtgcaccaggttcccaggttgtcgacggctactaccagcgcgcagac1584CysAlaProGlySerGlnValValAspGlyTyrTyrGlnArgAlaAsp515520525ctctggaaggaccttgaggccatgggcttctacctctccggcttcggc1632LeuTrpLysAspLeuGluAlaMetGlyPheTyrLeuSerGlyPheGly530535540tgcaccacctgtattggtaactccggcccactgccagaggaaatctcc1680CysThrThrCysIleGlyAsnSerGlyProLeuProGluGluIleSer545550555560gctgcgatcaacgagcacgacctgaccgcaaccgcagttttgtccggt1728AlaAlaIleAsnGluHisAspLeuThrAlaThrAlaValLeuSerGly565570575aaccgtaacttcgagggacgtatctcccctgacgttaagatgaactac1776AsnArgAsnPheGluGlyArgIleSerProAspValLysMetAsnTyr580585590ctggcatccccaatcatggtcattgcttacgcaatcgctggcaccatg1824LeuAlaSerProIleMetValIleAlaTyrAlaIleAlaGlyThrMet595600605gacttcgacttcgagaacgaagctcttggacaggaccaggacggcaac1872AspPheAspPheGluAsnGluAlaLeuGlyGlnAspGlnAspGlyAsn610615620gacgtcttcctgaaggacatctggccttccaccgaggaaatcgaagac1920AspValPheLeuLysAspIleTrpProSerThrGluGluIleGluAsp625630635640accatccagcaggcaatctcccgtgagctttacgaagctgactacgca1968ThrIleGlnGlnAlaIleSerArgGluLeuTyrGluAlaAspTyrAla645650655gatgtcttcaagggtgacaagcagtggcaggaactcgatgttcctacc2016AspValPheLysGlyAspLysGlnTrpGlnGluLeuAspValProThr660665670ggtgacaccttcgagtgggacgagaactccacctacatccgcaaggca2064GlyAspThrPheGluTrpAspGluAsnSerThrTyrIleArgLysAla675680685ccttacttcgacggcatgcctgtcgagccagtggcagtcaccgacatc2112ProTyrPheAspGlyMetProValGluProValAlaValThrAspIle690695700cagggcgcacgcgttctggctaagctcggcgactctgtcaccaccgac2160GlnGlyAlaArgValLeuAlaLysLeuGlyAspSerValThrThrAsp705710715720cacatctcccctgcttcctccattaagccaggtacccctgcagctcag2208HisIleSerProAlaSerSerIleLysProGlyThrProAlaAlaGln725730735tacttggatgagcacggtgtggaacgccacgactacaactccctgggt2256TyrLeuAspGluHisGlyValGluArgHisAspTyrAsnSerLeuGly740745750tccaggcgtggtaaccacgaggtcatgatgcgcggcaccttcgccaac2304SerArgArgGlyAsnHisGluValMetMetArgGlyThrPheAlaAsn755760765atccgcctccagaaccagctggttgacatcgcaggtggctacacccgc2352IleArgLeuGlnAsnGlnLeuValAspIleAlaGlyGlyTyrThrArg770775780gacttcacccaggagggtgctccacaggcgttcatctacgacgcttcc2400AspPheThrGlnGluGlyAlaProGlnAlaPheIleTyrAspAlaSer785790795800gtcaactacaaggctgctggcattccgctggtcgtcttgggcggcaag2448ValAsnTyrLysAlaAlaGlyIleProLeuValValLeuGlyGlyLys805810815gagtacggcaccggttcttcccgtgactgggcagctaagggcactaac2496GluTyrGlyThrGlySerSerArgAspTrpAlaAlaLysGlyThrAsn820825830ctgctcggaattcgcgcagttatcaccgagtccttcgagcgtattcac2544LeuLeuGlyIleArgAlaValIleThrGluSerPheGluArgIleHis835840845cgctccaacctcatcggtatgggcgttgtcccactgcagttccctgca2592ArgSerAsnLeuIleGlyMetGlyValValProLeuGlnPheProAla850855860ggcgaatcccacgagtccctgggccttgacggcaccgagaccttcgac2640GlyGluSerHisGluSerLeuGlyLeuAspGlyThrGluThrPheAsp865870875880atcaccggactgaccgcactcaacgagggcgagactcctaagactgtc2688IleThrGlyLeuThrAlaLeuAsnGluGlyGluThrProLysThrVal885890895aaggtcaccgcaaccaaggagaacggcgacgtcgtcgagttcgacgca2736LysValThrAlaThrLysGluAsnGlyAspValValGluPheAspAla900905910gttgtccgcatcgacaccccaggtgaggctgactactaccgccacggc2784ValValArgIleAspThrProGlyGluAlaAspTyrTyrArgHisGly915920925ggcatcctgcagtacgtgctgcgtcagatggctgcttcttctaag2829GlyIleLeuGlnTyrValLeuArgGlnMetAlaAlaSerSerLys930935940<210>84<211>943<212>PRT<213>谷氨酸棒杆菌<400>84MetGluLeuThrValThraluSerLysAsnSerPheAsnAlaLysSer151015ThrLeuGluValGlyAspLysSerTyrAspTyrPheAlaLeuSerAla202530ValProGlyMetGluLysLeuProTyrSerLeuLysValLeuGlyGlu354045AsnLeuLeuArgThrGluAspGlyAlaAsnIleThrAsnGluHisIle505560GluAlaIleAlaAsnTrpAspAlaSerSerAspProSerIleGluIle65707580GlnPheThrProAlaArgValLeuMetGlnAspPheThrGlyValPro859095CysValValAspLeuAlaThrMetArgGluAlaValAlaAlaLeuGly100105110GlyAspProAsnAspValAsnProLeuAsnProAlaGluMetValIle115120125AspHisSerValIleValGluAlaPheGlyArgProAspAlaLeuAla130135140LysAsnValGluIleGluTyrGluArgAsnGluGluArgTyrGlnPhe145150155160LeuArgTrpGlySerGluSerPheSerAsnPheArgValValProPro165170175GlyThrGlyIleValHisGlnValAsnIleGluTyrLeuAlaArgVal180185190ValPheAspAsnGluGlyLeuAlaTyrProAspThrCysIleGlyThr195200205AspSerHisThrThrMetGluAsnGlyLeuGlyIleLeuGlyTrpGly210215220ValGlyGlyIleGluAlaGluAlaAlaMetLeuGlyGlnProValSer225230235240MetLeuIleProArgValValGlyPheLysLeuThrGlyGluIlePro245250255ValGlyValThrAlaThrAspValValLeuThrIleThrGluMetLeu260265270ArgAspHisGlyValValGlnLysPheValGluPheTyrGlySerGly275280285ValLysAlaValProLeuAlaAsnArgAlaThrIleGlyAsnMetSer290295300ProGluPheGlySerThrCysAlaMetPheProIleAspGluGluThr305310315320ThrLysTyrLeuArgLeuThrGlyArgProGluGluGlnValAlaLeu325330335ValGluAlaTyrAlaLysAlaGlnGlyMetTrpLeuAspGluAspThr340345350ValGluAlaGluTyrSerGluTyrLeuGluLeuAspLeuSerThrVal355360365ValProSerIleAlaGlyProLysArgProGlnAspArgIleLeuLeu370375380SerGluAlaLysGluGlnPheArgLysAspLeuProThrTyrThrAsp385390395400AspAlaValSerValAspThrSerIleProAlaThrArgMetValAsn405410415GluGlyGlyGlyGlnProGluGlyGlyValGluAlaAspAsnTyrAsn420425430AlaSerTrpAlaGlySerGlyGluSerLeuAlaThrGlyAlaGluGly435440445ArgProSerLysProValThrValAlaSerProGlnGlyGlyGluTyr450455460ThrIleAspHisGlyMetValAlaIleAlaSerIleThrSerCysThr465470475480AsnThrSerAsnProSerValMetIleGlyAlaGlyLeuIleAlaArg485490495LysAlaAlaGluLysGlyLeuLysSerLysProTrpValLysThrIle500505510CysAlaProGlySerGlnValValAspGlyTyrTyrGlnArgAlaAsp515520525LeuTrpLysAspLeuGluAlaMetGlyPheTyrLeuSerGlyPheGly530535540CysThrThrCysIleGlyAsnSerGlyProLeuProGluGluIleSer545550555560AlaAlaIleAsnGluHisAspLeuThrAlaThrAlaValLeuSerGly565570575AsnArgAsnPheGluGlyArgIleSerProAspValLysMetAsnTyr580585590LeuAlaSerProIleMetValIleAlaTyrAlaIleAlaGlyThrMet595600605AspPheAspPheGluAsnGluAlaLeuGlyGlnAspGlnAspGlyAsn610615620AspValPheLeuLysAspIleTrpProSerThrGluGluIleGluAsp625630635640ThrIleGlnGlnAlaIleSerArgGluLeuTyrGluAlaAspTyrAla645650655AspValPheLysGlyAspLysGlnTrpGlnGluLeuAspValProThr660665670GlyAspThrPheGluTrpAspGluAsnSerThrTyrIleArgLysAla675680685ProTyrPheAspGlyMetProValGluProValAlaValThrAspIle690695700GlnGlyAlaArgValLeuAlaLysLeuGlyAspSerValThrThrAsp705710715720HisIleSerProAlaSerSerIleLysProGlyThrProAlaAlaGln725730735TyrLeuAspGluHisGlyValGluArgHisAspTyrAsnSerLeuGly740745750SerArgArgGlyAsnHisGluValMetMetArgGlyThrPheAlaAsn755760765IleArgLeuGlnAsnGlnLeuValAspIleAlaGlyGlyTyrThrArg770775780AspPheThrGlnGluGlyAlaProGlnAlaPheIleTyrAspAlaSer785790795800ValAsnTyrLysAlaAlaGlyIleProLeuValValLeuGlyGlyLys805810815GluTyrGlyThrGlySerSerArgAspTrpAlaAlaLysGlyThrAsn820825830LeuLeuGlyIleArgAlaValIleThrGluSerPheGluArgIleHis835840845ArgSerAsnLeuIleGlyMetGlyValValProLeuGlnPheProAla850855860GlyGluSerHisGluSerLeuGlyLeuAspGlyThrGluThrPheAsp865870875880IleThrGlyLeuThrAlaLeuAsnGluGlyGluThrProLysThrVal885890895LysValThrAlaThrLysGluAsnGlyAspValValGluPheAspAla900905910ValValArgIleAspThrProGlyGluAlaAspTyrTyrArgHisGly915920925GlyIleLeuGlnTyrValLeuArgGlnMetAlaAlaSerSerLys930935940<210>85<211>1293<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1293)<223>RXA02175W<400>85gtggctactgataacaacaaggctgtcctgcactaccccggtggcgag48MetAlaThrAspAsnAsnLysAlaValLeuHisTyrProGlyGlyGlu151015ttcgaaatggacatcatcgaggcttctgagggtaacaacggtgttgtc96PheGluMetAspIleIleGluAlaSerGluGlyAsnAsnGlyValVal202530ctgggcaagatgctgtctgagactggactgatcacttttgacccaggt144LeuGlyLysMetLeuSerGluThrGlyLeuIleThrPheAspProGly354045tatgtgagcactggctccaccgagtcgaagatcacctacatcgatggc192TyrValSerThrGlySerThrGluSerLysIleThrTyrIleAspGly505560gatgcgggaatcctgcgttaccgcggctatgacatcgctgatctggct240AspAlaGlyIleLeuArgTyrArgGlyTyrAspIleAlaAspLeuAla65707580gagaatgccaccttcaacgaggtttcttacctacttatcaacggtgag288GluAsnAlaThrPheAsnGluValSerTyrLeuLeuIleAsnGlyGlu859095ctaccaaccccagatgagcttcacaagtttaacgacgagattcgccac336LeuProThrProAspGluLeuHisLysPheAsnAspGluIleArgHis100105110cacacccttctggacgaggacttcaagtcccagttcaacgtgttccca384HisThrLeuLeuAspGluAspPheLysSerGlnPheAsnValPhePro115120125cgcgacgctcacccaatggcaaccttggcttcctcggttaacattttg432ArgAspAlaHisProMetAlaThrLeuAlaSerSerValAsnIleLeu130135140tctacctactaccaggaccagctgaacccactcgatgaggcacagctt480SerThrTyrTyrGlnAspGlnLeuAsnProLeuAspGluAlaGlnLeu145150155160gataaggcaaccgttcgcctcatggcaaaggttccaatgctggctgcg528AspLysAlaThrValArgLeuMetAlaLysValProMetLeuAlaAla165170175tacgcacaccgcgcacgcaagggtgctccttacatgtacccagacaac576TyrAlaHisArgAlaArgLysGlyAlaProTyrMetTyrProAspAsn180185190tccctcaatgcgcgtgagaacttcctgcgcatgatgttcggttaccca624SerLeuAsnAlaArgGluAsnPheLeuArgMetMetPheGlyTyrPro195200205accgagccatacgagatcgacccaatcatggtcaaggctctggacaag672ThrGluProTyrGluIleAspProIleMetValLysAlaLeuAspLys210215220ctgctcatcctgcacgctgaccacgagcagaactgctccacctccacc720LeuLeuIleLeuHisAlaAspHisGluGlnAsnCysSerThrSerThr225230235240gttcgtatgatcggttccgcacaggccaacatgtttgtctccatcgct768ValArgMetIleGlySerAlaGlnAlaAsnMetPheValSerIleAla245250255ggtggcatcaacgctctgtccggcccactgcacggtggcgcaaaccag816GlyGlyIleAsnAlaLeuSerGlyProLeuHisGlyGlyAlaAsnGln260265270gctgttctggagatgctcgaagacatcaagagcaaccacggtggcgac864AlaValLeuGluMetLeuGluAspIleLysSerAsnHisGlyGlyAsp275280285gcaaccgagttcatgaacaaggtcaagaacaaggaagacggcgtccgc912AlaThrGluPheMetAsnLysValLysAsnLysGluAspGlyValArg290295300ctcatgggcttcggacaccgcgtttacaagaactacgatccacgtgca960LeuMetGlyPheGlyHisArgValTyrLysAsnTyrAspProArgAla305310315320gcaatcgtcaaggagaccgcacacgagatcctcgagcacctcggtggc1008AlaIleValLysGluThrAlaHisGluIleLeuGluHisLeuGlyGly325330335gacgatcttctggatctggcaatcaagctggaagaaattgcactggct1056AspAspLeuLeuAspLeuAlaIleLysLeuGluGluIleAlaLeuAla340345350gatgattacttcatctcccgcaagctctacccgaacgtagacttctac1104AspAspTyrPheIleSerArgLysLeuTyrProAsnValAspPheTyr355360365accggcctgatctaccgcgcaatgggcttcccaactgacttcttcacc1152ThrGlyLeuIleTyrArgAlaMetGlyPheProThrAspPhePheThr370375380gtattgttcgcaatcggtcgtctgccaggatggatcgctcactaccgc1200ValLeuPheAlaIleGlyArgLeuProGlyTrpIleAlaHisTyrArg385390395400gagcagctcggtgcagcaggcaacaagatcaaccgcccacgccaggtc1248GluGlnLeuGlyAlaAlaGlyAsnLysIleAsnArgProArgGlnVal405410415tacaccggcaacgaatcccgcaagttggttcctcgcgaggagcgc1293TyrThrGlyAsnGluSerArgLysLeuValProArgGluGluArg420425430<210>86<211>431<212>PRT<213>谷氨酸棒杆菌<400>86MetAlaThrAspAsnAsnLysAlaValLeuHisTyrProGlyGlyGlu151015PheGluMetAspIleIleGluAlaSerGluGlyAsnAsnGlyValVal202530LeuGlyLysMetLeuSerGluThrGlyLeuIleThrPheAspProGly354045TyrValSerThrGlySerThrGluSerLysIleThrTyrIleAspGly505560AspAlaGlyIleLeuArgTyrArgGlyTyrAspIleAlaAspLeuAla65707580GluAsnAlaThrPheAsnGluValSerTyrLeuLeuIleAsnGlyGlu859095LeuProThrProAspGluLeuHisLysPheAsnAspGluIleArgHis100105110HisThrLeuLeuAspGluAspPheLysSerGlnPheAsnValPhePro115120125ArgAspAlaHisProMetAlaThrLeuAlaSerSerValAsnIleLeu130135140SerThrTyrTyrGlnAspGlnLeuAsnProLeuAspGluAlaGlnLeu145150155160AspLysAlaThrValArgLeuMetAlaLysValProMetLeuAlaAla165170175TyrAlaHisArgAlaArgLysGlyAlaProTyrMetTyrProAspAsn180185190SerLeuAsnAlaArgGluAsnPheLeuArgMetMetPheGlyTyrPro195200205ThrGluProTyrGluIleAspProIleMetValLysAlaLeuAspLys210215220LeuLeuIleLeuHisAlaAspHisGluGlnAsnCysSerThrSerThr225230235240ValArgMetIleGlySerAlaGlnAlaAsnMetPheValSerIleAla245250255GlyGlyIleAsnAlaLeuSerGlyProLeuHisGlyGlyAlaAsnGln260265270AlaValLeuGluMetLeuGluAspIleLysSerAsnHisGlyGlyAsp275280285AlaThrGluPheMetAsnLysValLysAsnLysGluAspGlyValArg290295300LeuMetGlyPheGlyHisArgValTyrLysAsnTyrAspProArgAla305310315320AlaIleValLysGluThrAlaHisGluIleLeuGluHisLeuGlyGly325330335AspAspLeuLeuAspLeuAlaIleLysLeuGluGluIleAlaLeuAla340345350AspAspTyrPheIleSerArgLysLeuTyrProAsnValAspPheTyr355360365ThrGlyLeuIleTyrArgAlaMetGlyPheProThrAspPhePheThr370375380ValLeuPheAlaIleGlyArgLeuProGlyTrpIleAlaHisTyrArg385390395400GluGlnLeuGlyAlaAlaGlyAsnLysIleAsnArgProArgGlnVal405410415TyrThrGlyAsnGluSerArgLysLeuValProArgGluGluArg420425430<210>87<211>1311<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1311)<223>RXA02175E<400>87atgtttgaaagggatatcgtggctactgataacaacaaggctgtcctg48MetPheGluArgAspIleValAlaThrAspAsnAsnLysAlaValLeu151015cactaccccggtggcgagttcgaaatggacatcatcgaggcttctgag96HisTyrProGlyGlyGluPheGluMetAspIleIleGluAlaSerGlu202530ggtaacaacggtgttgtcctgggcaagatgctgtctgagactggactg144GlyAsnAsnGlyValValLeuGlyLysMetLeuSerGluThrGlyLeu354045atcacttttgacccaggttatgtgagcactggctccaccgagtcgaag192IleThrPheAspProGlyTyrValSerThrGlySerThrGluSerLys505560atcacctacatcgatggcgatgcgggaatcctgcgttaccgcggctat240IleThrTyrIleAspGlyAspAlaGlyIleLeuArgTyrArgGlyTyr65707580gacatcgctgatctggctgagaatgccaccttcaacgaggtttcttac288AspIleAlaAspLeuAlaGluAsnAlaThrPheAsnGluValSerTyr859095ctacttatcaacggtgagctaccaaccccagatgagcttcacaagttt336LeuLeuIleAsnGlyGluLeuProThrProAspGluLeuHisLysPhe100105110aacgacgagattcgccaccacacccttctggacgaggacttcaagtcc384AsnAspGluIleArgHisHisThrLeuLeuAspGluAspPheLysSer115120125cagttcaacgtgttcccacgcgacgctcacccaatggcaaccttggct432GlnPheAsnValPheProArgAspAlaHisProMetAlaThrLeuAla130135140tcctcggttaacattttgtctacctactaccaggaccagctgaaccca480SerSerValAsnIleLeuSerThrTyrTyrGlnAspGlnLeuAsnPro145150155160ctcgatgaggcacagcttgataaggcaaccgttcgcctcatggcaaag528LeuAspGluAlaGlnLeuAspLysAlaThrValArgLeuMetAlaLys165170175gttccaatgctggctgcgtacgcacaccgcgcacgcaagggtgctcct576ValProMetLeuAlaAlaTyrAlaHisArgAlaArgLysGlyAlaPro180185190tacatgtacccagacaactccctcaatgcgcgtgagaacttcctgcgc624TyrMetTyrProAspAsnSerLeuAsnAlaArgGluAsnPheLeuArg195200205atgatgttcggttacccaaccgagccatacgagatcgacccaatcatg672MetMetPheGlyTyrProThrGluProTyrGluIleAspProIleMet210215220gtcaaggctctggacaagctgctcatcctgcacgctgaccacgagcag720ValLysAlaLeuAspLysLeuLeuIleLeuHisAlaAspHisGluGln225230235240aactgctccacctccaccgttcgtatgatcggttccgcacaggccaac768AsnCysSerThrSerThrValArgMetIleGlySerAlaGlnAlaAsn245250255atgtttgtctccatcgctggtggcatcaacgctctgtccggcccactg816MetPheValSerIleAlaGlyGlyIleAsnAlaLeuSerGlyProLeu260265270cacggtggcgcaaaccaggctgttctggagatgctcgaagacatcaag864HisGlyGlyAlaAsnGlnAlaValLeuGluMetLeuGluAspIleLys275280285agcaaccacggtggcgacgcaaccgagttcatgaacaaggtcaagaac912SerAsnHisGlyGlyAspAlaThrGluPheMetAsnLysValLysAsn290295300aaggaagacggcgtccgcctcatgggcttcggacaccgcgtttacaag960LysGluAspGlyValArgLeuMetGlyPheGlyHisArgValTyrLys305310315320aactacgatccacgtgcagcaatcgtcaaggagaccgcacacgagatc1008AsnTyrAspProArgAlaAlaIleValLysGluThrAlaHisGluIle325330335ctcgagcacctcggtggcgacgatcttctggatctggcaatcaagctg1056LeuGluHisLeuGlyGlyAspAspLeuLeuAspLeuAlaIleLysLeu340345350gaagaaattgcactggctgatgattacttcatctcccgcaagctctac1104GluGluIleAlaLeuAlaAspAspTyrPheIleSerArgLysLeuTyr355360365ccgaacgtagacttctacaccggcctgatctaccgcgcaatgggcttc1152ProAsnValAspPheTyrThrGlyLeuIleTyrArgAlaMetGlyPhe370375380ccaactgacttcttcaccgtattgttcgcaatcggtcgtctgccagga1200ProThrAspPhePheThrValLeuPheAlaIleGlyArgLeuProGly385390395400tggatcgctcactaccgcgagcagctcggtgcagcaggcaacaagatc1248TrpIleAlaHisTyrArgGluGlnLeuGlyAlaAlaGlyAsnLysIle405410415aaccgcccacgccaggtctacaccggcaacgaatcccgcaagttggtt1296AsnArgProArgGlnValTyrThrGlyAsnGluSerArgLysLeuVal420425430cctcgcgaggagcgc1311ProArgGluGluArg435<210>88<211>437<212>PRT<213>谷氨酸棒杆菌<400>88MetPheGluArgAspIleValAlaThrAspAsnAsnLysAlaValLeu151015HisTyrProGlyGlyGluPheGluMetAspIleIleGluAlaSerGlu202530GlyAsnAsnGlyValValLeuGlyLysMetLeuSerGluThrGlyLeu354045IleThrPheAspProGlyTyrValSerThrGlySerThrGluSerLys505560IleThrTyrIleAspGlyAspAlaGlyIleLeuArgTyrArgGlyTyr65707580AspIleAlaAspLeuAlaGluAsnAlaThrPheAsnGluValSerTyr859095LeuLeuIleAsnGlyGluLeuProThrProAspGluLeuHisLysPhe100105110AsnAspGluIleArgHisHisThrLeuLeuAspGluAspPheLysSer115120125GlnPheAsnValPheProArgAspAlaHisProMetAlaThrLeuAla130135140SerSerValAsnIleLeuSerThrTyrTyrGlnAspGlnLeuAsnPro145150155160LeuAspGluAlaGlnLeuAspLysAlaThrValArgLeuMetAlaLys165170175ValProMetLeuAlaAlaTyrAlaHisArgAlaArgLysGlyAlaPro180185190TyrMetTyrProAspAsnSerLeuAsnAlaArgGluAsnPheLeuArg195200205MetMetPheGlyTyrProThrGluProTyrGluIleAspProIleMet210215220ValLysAlaLeuAspLysLeuLeuIleLeuHisAlaAspHisGluGln225230235240AsnCysSerThrSerThrValArgMetIleGlySerAlaGlnAlaAsn245250255MetPheValSerIleAlaGlyGlyIleAsnAlaLeuSerGlyProLeu260265270HisGlyGlyAlaAsnGlnAlaValLeuGluMetLeuGluAspIleLys275280285SerAsnHisGlyGlyAspAlaThrGluPheMetAsnLysValLysAsn290295300LysGluAspGlyValArgLeuMetGlyPheGlyHisArgValTyrLys305310315320AsnTyrAspProArgAlaAlaIleValLysGluThrAlaHisGluIle325330335LeuGluHisLeuGlyGlyAspAspLeuLeuAspLeuAlaIleLysLeu340345350GluGluIleAlaLeuAlaAspAspTyrPheIleSerArgLysLeuTyr355360365ProAsnValAspPheTyrThrGlyLeuIleTyrArgAlaMetGlyPhe370375380ProThrAspPhePheThrValLeuPheAlaIleGlyArgLeuProGly385390395400TrpIleAlaHisTyrArgGluGlnLeuGlyAlaAlaGlyAsnLysIle405410415AsnArgProArgGlnValTyrThrGlyAsnGluSerArgLysLeuVal420425430ProArgGluGluArg435<210>89<211>3663<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(3663)<223>RXA04186K<400>89atgtctacttcagttacttcaccagcccacaacaacgcacattcctcc48MetSerThrSerValThrSerProAlaHisAsnAsnAlaHisSerSer151015gaatttttggatgcgttggcaaaccatgtgttgatcggcgacggcgcc96GluPheLeuAspAlaLeuAlaAsnHisValLeuIleGlyAspGlyAla202530atgggcacccagctccaaggctttgacctggacgtggaaaaggatttc144MetGlyThrGlnLeuGlnGlyPheAspLeuAspValGluLysAspPhe354045cttgatctggaggggtgtaatgagattctcaacgacacccgccctgat192LeuAspLeuGluGlyCysAsnGluIleLeuAsnAspThrArgProAsp505560gtgttgaggcagattcaccgcgcctactttgaggcgggagctgacttg240ValLeuArgGlnIleHisArgAlaTyrPheGluAlaGlyAlaAspLeu65707580gttgagaccaatacttttggttgcaacctgccgaacttggcggattat288ValGluThrAsnThrPheGlyCysAsnLeuProAsnLeuAlaAspTyr859095gacatcgctgatcgttgccgtgagcttgcctacaagggcactgcagtg336AspIleAlaAspArgCysArgGluLeuAlaTyrLysGlyThrAlaVal100105110gctagggaagtggctgatgagatggggccgggccgaaacggcatgcgg384AlaArgGluValAlaAspGluMetGlyProGlyArgAsnGlyMetArg115120125cgtttcgtggttggttccctgggacctggaacgaagcttccatcgctg432ArgPheValValGlySerLeuGlyProGlyThrLysLeuProSerLeu130135140ggccargcaccgtatgcagatttgcgtgggcactacaaggaagcagcg480GlyHisAlaProTyrAlaAspLeuArgGlyHisTyrLysGluAlaAla145150155160cttggcatcatcgacggtggtggcgatgcctttttgattgagactgct528LeuGlyIleIleAspGlyGlyGlyAspAlaPheLeuIleGluThrAla165170175caggacttgattcaggtcaaggctgcggttcacggcgttcaagatgcc576GlnAspLeuLeuGlnValLysAlaAlaValHisGlyValGlnAspAla180185190atggctgaacttgatacattcttgcccattatttgccacgtcaccgta624MetAlaGluLeuAspThrPheLeuProIleIleCysHisValThrVal195200205gagaccaccggcaccatgctcatgggttctgagatcggtgccgcgttg672GluThrThrGlyThrMetLeuMetGlySerGluIleGlyAlaAlaLeu210215220acagcgctgcagccactgggtatcgacatgattggtctgaactgcgcc720ThrAlaLeuGlnProLeuGlyIleAspMetIleGlyLeuAsnCysAla225230235240accggcccagatgagatgagcgagcacctgcgttacctgtccaagcac768ThrGlyProAspGluMetSerGluHisLeuArgTyrLeuSerLysHis245250255gccgatattcctgtgtcggtgatgcctaacgcaggtcttcctgtcctg816AlaAspIleProValSerValMetProAsnAlaGlyLeuProValLeu260265270ggtaaaaacggtgcagaatacccacttgaggctgaggatttggcgcag864GlyLysAsnGlyAlaGluTyrProLeuGluAlaGluAspLeuAlaGln275280285gcgctggctggattcgtctccgaatatggcctgtccatggtgggtggt912AlaLeuAlaGlyPheValSerGluTyrGlyLeuSerMetValGlyGly290295300tgttgtggcaccacacctgagcacatccgtgcggtccgcgatgcggtg960CysCysGlyThrThrProGluHisIleArgAlaValArgAspAlaVal305310315320gttggtgttccagagcaggaaacctccacactgaccaagatccctgca1008ValGlyValProGluGlnGluThrSerThrLeuThrLysIleProAla325330335ggccctgttgagcaggcctcccgcgaggtggagaaagaggactccgtc1056GlyProValGluGlnAlaSerArgGluValGluLysGluAspSerVal340345350gcgtcgctgtacacctcggtgccattgtcccaggaaaccggcatttcc1104AlaSerLeuTyrThrSerValProLeuSerGlnGluThrGlyIleSer355360365atgatcggtgagcgcaccaactccaacggttccaaggcattccgtgag1152MetIleGlyGluArgThrAsnSerAsnGlySerLysAlaPheArgGlu370375380gcaatgctgtctggcgattgggaaaagtgtgtggatattgccaagcag1200AlaMetLeuSerGlyAspTrpGluLysCysValAspIleAlaLysGln385390395400caaacccgcgatggtgcacacatgctggatctttgtgtggattacgtg1248GlnThrArgAspGlyAlaHisMetLeuAspLeuCysValAspTyrVal405410415ggacgagacggcaccgccgatatggcgaccttggcagcacttcttgct1296GlyArgAspGlyThrAlaAspMetAlaThrLeuAlaAlaLeuLeuAla420425430accagctccactttgccaatcatgattgactccaccgagccagaggtt1344ThrSerSerThrLeuProIleMetIleAspSerThrGluProGluVal435440445attcgcacaggccttgagcacttgggtggacgaagcatcgttaactcc1392IleArgThrGlyLeuGluHisLeuGlyGlyArgSerIleValAsnSer450455460gtcaactttgaagacggcgatggccctgagtcccgctaccagcgcatc1440ValAsnPheGluAspGlyAspGlyProGluSerArgTyrGlnArgIle465470475480atgaaactggtaaagcagcacggtgcggccgtggttgcgctgaccatt1488MetLysLeuValLysGlnHisGlyAlaAlaValValAlaLeuThrIle485490495gatgaggaaggccaggcacgtaccgctgagcacaaggtgcgcattgct1536AspGluGluGlyGlnAlaArgThrAlaGluHisLysValArgIleAla500505510aaacgactgattgacgatatcaccggcagctacggcctggatatcaaa1584LysArgLeuIleAspAspIleThrGlySerTyrGlyLeuAspIleLys515520525gacatcgttgtggactgcctgaccttcccgatctctactggccaggaa1632AspIleValValAspCysLeuThrPheProIleSerThrGlyGlnGlu530535540gaaaccaggcgagatggcattgaaaccatcgaagccatccgcgagctg1680GluThrArgArgAspGlyIleGluThrIleGluAlaIleArgGluLeu545550555560aagaagctctacccagaaatccacaccaccctgggtctgtccaatatt1728LysLysLeuTyrProGluIleHisThrThrLeuGlyLeuSerAsnIle565570575tccttcggcctgaaccctgctgcacgccaggttcttaactctgtgttc1776SerPheGlyLeuAsnProAlaAlaArgGlnValLeuAsnSerValPhe580585590ctcaatgagtgcattgaggctggtctggactctgcgattgcgcacagc1824LeuAsnGluCysIleGluAlaGlyLeuAspSerAlaIleAlaHisSer595600605tccaagattttgccgatgaaccgcattgatgatcgccagcgcgaagtg1872SerLysIleLeuProMetAsnArgIleAspAspArgGlnArgGluVal610615620gcgttggatatggtctatgatcgccgcaccgaggattacgatccgctg1920AlaLeuAspMetValTyrAspArgArgThrGluAspTyrAspProLeu625630635640caggaattcatgcagctgtttgagggcgtttctgctgccgatgccaag1968GlnGluPheMetGlnLeuPheGluGlyValSerAlaAlaAspAlaLys645650655gatgctcgcgctgaacagctggccgctatgcctttgtttgagcgtttg2016AspAlaArgAlaGluGlnLeuAlaAlaMetProLeuPheGluArgLeu660665670gcacagcgcatcatcgacggcgataagaatggccttgaggatgatctg2064AlaGlnArgIleIleAspGlyAspLysAsnGlyLeuGluAspAspLeu675680685gaagcaggcatgaaggagaagtctcctattgcgatcatcaacgaggac2112GluAlaGlyMetLysGluLysSerProIleAlaIleIleAsnGluAsp690695700cttctcaacggcatgaagaccgtgggtgagctgtttggttccggacag2160LeuLeuAsnGlyMetLysThrValGlyGluLeuPheGlySerGlyGln705710715720atgcagctgccattcgtgctgcaatcggcagaaaccatgaaaactgcg2208MetGlnLeuProPheValLeuGlnSerAlaGluThrMetLysThrAla725730735gtggcctatttggaaccgttcatggaagaggaagcagaagctaccgga2256ValAlaTyrLeuGluProPheMetGluGluGluAlaGluAlaThrGly740745750tctgcgcaggcagagggcaagggcaaaatcgtcgtggccaccgtcaag2304SerAlaGlnAlaGluGlyLysGlyLysIleValValAlaThrValLys755760765ggtgacgtgcacgatatcggcaagaacttggtggacatcattttgtcc2352GlyAspValHisAspIleGlyLysAsnLeuValAspIleIleLeuSer770775780aacaacggttacgacgtggtgaacttgggcatcaagcagccactgtcc2400AsnAsnGlyTyrAspValValAsnLeuGlyIleLysGlnProLeuSer785790795800gccatgttggaagcagcggaagaacacaaagcagacgtcatcggcatg2448AlaMetLeuGluAlaAlaGluGluHisLysAlaAspValIleGlyMet805810815tcgggacttcttgtgaagtccaccgtggtgatgaaggaaaaccttgag2496SerGlyLeuLeuValLysSerThrValValMetLysGluAsnLeuGlu820825830gagatgaacaacgccggcgcatccaattacccagtcattttgggtggc2544GluMetAsnAsnAlaGlyAlaSerAsnTyrProValIleLeuGlyGly835840845gctgcgctgacgcgtacctacgtggaaaacgatctcaacgaggtgtac2592AlaAlaLeuThrArgThrTyrValGluAsnAspLeuAsnGluValTyr850855860accggtgaggtgtactacgcccgtgatgctttcgagggcctgcgcctg2640ThrGlyGluValTyrTyrAlaArgAspAlaPheGluGlyLeuArgLeu865870875880atggatgaggtgatggcagaaaagcgtggtgaaggacttgatcccaac2688MetAspGluValMetAlaGluLysArgGlyGluGlyLeuAspProAsn885890895tcaccagaagctattgagcaggcgaagaagaaggcggaacgtaaggct2736SerProGluAlaIleGluGlnAlaLysLysLysAlaGluArgLysAla900905910cgtaatgagcgttcccgcaagattgccgcggagcgtaaagctaatgcg2784ArgAsnGluArgSerArgLysIleAlaAlaGluArgLysAlaAsnAla915920925gctcccgtgattgttccggagcgttctgatgtctccaccgatactcca2832AlaProValIleValProGluArgSerAspValSerThrAspThrPro930935940accgcggcaccaccgttctggggaacccgcattgtcaagggtctgccc2880ThrAlaAlaProProPheTrpGlyThrArgIleValLysGlyLeuPro945950955960ttggcggagttcttgggcaaccttgatgagcgcgccttgttcatgggg2928LeuAlaGluPheLeuGlyAsnLeuAspGluArgAlaLeuPheMetGly965970975cagtggggtctgaaatccacccgcggcaacgagggtccaagctatgag2976GlnTrpGlyLeuLysSerThrArgGlyAsnGluGlyProSerTyrGlu980985990gatttggtggaaactgaaggccgaccacgcctgcgctactggctggat3024AspLeuValGluThrGluGlyArgProArgLeuArgTyrTrpLeuAsp99510001005cgcctgaagtctgagggcattttggaccacgtggccttggtgtatggc3072ArgLeuLysSerGluGlyIleLeuAspHisValAlaLeuValTyrGly101010151020tacttcccagcggtcgcggaaggcgatgacgtggtgatcttggaatcc3120TyrPheProAlaValAlaGluGlyAspAspValValIleLeuGluSer1025103010351040ccggatccacacgcagccgaacgcatgcgctttagcttcccacgccag3168ProAspProHisAlaAlaGluArgMetArgPheSerPheProArgGln104510501055cagcgcggcaggttcttgtgcatcgcggatttcattcgcccacgcgag3216GlnArgGlyArgPheLeuCysIleAlaAspPheIleArgProArgGlu106010651070caagctgtcaaggacggccaagtggacgtcatgccattccagctggtc3264GlnAlaValLysAspGlyGlnValAspValMetProPheGlnLeuVal107510801085accatgggtaatcctattgctgatttcgccaacgagttgttcgcagcc3312ThrMetGlyAsnProIleAlaAspPheAlaAsnGluLeuPheAlaAla109010951100aatgaataccgcgagtacttggaagttcacggcatcggcgtgcagctc3360AsnGluTyrArgGluTyrLeuGluValHisGlyIleGlyValGlnLeu1105111011151120accgaagcattggccgagtactggcactcccgagtgcgcagcgaactc3408ThrGluAlaLeuAlaGluTyrTrpHisSerArgValArgSerGluLeu112511301135aagctgaacgacggtggatctgtcgctgattttgatccagaagacaag3456LysLeuAsnAspGlyGlySerValAlaAspPheAspProGluAspLys114011451150accaagttcttcgacctggattaccgcggcgcccgcttctcctttggt3504ThrLysPhePheAspLeuAspTyrArgGlyAlaArgPheSerPheGly115511601165tacggttctygccctgatctggaagaccgcgcaaagctggtggaattg3552TyrGlySerCysProAspLeuGluAspArgAlaLysLeuValGluLeu117011751180ctcgagccaggccgtatcggcgtggagttgtccgaggaactccagctg3600LeuGluProGlyArgIleGlyValGluLeuSerGluGluLeuGlnLeu1185119011951200cacccagagcagtccacagacgcgtttgtgctctaccacccagaggca3648HisProGluGlnSerThrAspAlaPheValLeuTyrHisProGluAla120512101215aagtactttaacgtc3663LysTyrPheAsnVal1220<210>90<211>1221<212>PRT<213>谷氨酸棒杆菌<400>90MetSerThrSerValThrSerProAlaHisAsnAsnAlaHisSerSer151015GluPheLeuAspAlaLeuAlaAsnHisValLeuIleGlyAspGlyAla202530MetGlyThrGlnLeuGlnGlyPheAspLeuAspValGluLysAspPhe354045LeuAspLeuGluGlyCysAsnGluIleLeuAsnAspThrArgProAsp505560ValLeuArgGlnIleHisArgAlaTyrPheGluAlaGlyAlaAspLeu65707580ValGluThrAsnThrPheGlyCysAsnLeuProAsnLeuAlaAspTyr859095AspIleAlaAspArgCysArgGluLeuAlaTyrLysGlyThrAlaVal100105110AlaArgGluValAlaAspGluMetGlyProGlyArgAsnGlyMetArg115120125ArgPheValValGlySerLeuGlyProGlyThrLysLeuProSerLeu130135140GlyHisAlaProTyrAlaAspLeuArgGlyHisTyrLysGluAlaAla145150155160LeuGlyIleIleAspGlyGlyGlyAspAlaPheLeuIleGluThrAla165170175GlnAspLeuLeuGlnValLysAlaAlaValHisGlyValGlnAspAla180185190MetAlaGluLeuAspThrPheLeuProIleIleCysHisValThrVal195200205GluThrThrGlyThrMetLeuMetGlySerGluIleGlyAlaAlaLeu210215220ThrAlaLeuGlnProLeuGlyIleAspMetIleGlyLeuAsnCysAla225230235240ThrGlyProAspGluMetSerGluHisLeuArgTyrLeuSerLysHis245250255AlaAspIleProValSerValMetProAsnAlaGlyLeuProValLeu260265270GlyLysAsnGlyAlaGluTyrProLeuGluAlaGluAspLeuAlaGln275280285AlaLeuAlaGlyPheValSerGluTyrGlyLeuSerMetValGlyGly290295300CysCysGlyThrThrProGluHisIleArgAlaValArgAspAlaVal305310315320ValGlyValProGluGlnGluThrSerThrLeuThrLysIleProAla325330335GlyProValGluGlnAlaSerArgGluValGluLysGluAspSerVal340345350AlaSerLeuTyrThrSerValProLeuSerGlnGluThrGlyIleSer355360365MetIleGlyGluArgThrAsnSerAsnGlySerLysAlaPheArgGlu370375380AlaMetLeuSerGlyAspTrpGluLysCysValAspIleAlaLysGln385390395400GlnThrArgAspGlyAlaHisMetLeuAspLeuCysValAspTyrVal405410415GlyArgAspGlyThrAlaAspMetAlaThrLeuAlaAlaLeuLeuAla420425430ThrSerSerThrLeuProIleMetIleAspSerThrGluProGluVal435440445IleArgThrGlyLeuGluHisLeuGlyGlyArgSerIleValAsnSer450455460ValAsnPheGluAspGlyAspGlyProGluSerArgTyrGlnArgIle465470475480MetLysLeuValLysGlnHisGlyAlaAlaValValAlaLeuThrIle485490495AspGluGluGlyGlnAlaArgThrAlaGluHisLysValArgIleAla500505510LysArgLeuIleAspAspIleThrGlySerTyrGlyLeuAspIleLys515520525AspIleValValAspCysLeuThrPheProIleSerThrGlyGlnGlu530535540GluThrArgArgAspGlyIleGluThrIleGluAlaIleArgGluLeu545550555560LysLysLeuTyrProGluIleHisThrThrLeuGlyLeuSerAsnIle565570575SerPheGlyLeuAsnProAlaAlaArgGlnValLeuAsnSerValPhe580585590LeuAsnGluCysIleGluAlaGlyLeuAspSerAlaIleAlaHisSer595600605SerLysIleLeuProMetAsnArgIleAspAspArgGlnArgGluVal610615620AlaLeuAspMetValTyrAspArgArgThrGluAspTyrAspProLeu625630635640GlnGluPheMetGlnLeuPheGluGlyValSerAlaAlaAspAlaLys645650655AspAlaArgAlaGluGlnLeuAlaAlaMetProLeuPheGluArgLeu660665670AlaGlnArgIleIleAspGlyAspLysAsnGlyLeuGluAspAspLeu675680685GluAlaGlyMetLysGluLysSerProIleAlaIleIleAsnGluAsp690695700LeuLeuAsnGlyMetLysThrValGlyGluLeuPheGlySerGlyGln705710715720MetGlnLeuProPheValLeuGlnSerAlaGluThrMetLysThrAla725730735ValAlaTyrLeuGluProPheMetGluGluGluAlaGluAlaThrGly740745750SerAlaGlnAlaGluGlyLysGlyLysIleValValAlaThrValLys755760765GlyAspValHisAspIleGlyLysAsnLeuValAspIleIleLeuSer770775780AsnAsnGlyTyrAspValValAsnLeuGlyIleLysGlnProLeuSer785790795800AlaMetLeuGluAlaAlaGluGluHisLysAlaAspValIleGlyMet805810815SerGlyLeuLeuValLysSerThrValValMetLysGluAsnLeuGlu820825830GluMetAsnAsnAlaGlyAlaSerAsnTyrProValIleLeuGlyGly835840845AlaAlaLeuThrArgThrTyrValGluAsnAspLeuAsnGluValTyr850855860ThrGlyGluValTyrTyrAlaArgAspAlaPheGluGlyLeuArgLeu865870875880MetAspGluValMetAlaGluLysArgGlyGluGlyLeuAspProAsn885890895SerProGluAlaIleGluGlnAlaLysLysLysAlaGluArgLysAla900905910ArgAsnGluArgSerArgLysIleAlaAlaGluArgLysAlaAsnAla915920925AlaProValIleValProGluArgSerAspValSerThrAspThrPro930935940ThrAlaAlaProProPheTrpGlyThrArgIleValLysGlyLeuPro945950955960LeuAlaGluPheLeuGlyAsnLeuAspGluArgAlaLeuPheMetGly965970975GlnTrpGlyLeuLysSerThrArgGlyAsnGluGlyProSerTyrGlu980985990AspLeuValGluThrGluGlyArgProArgLeuArgTyrTrpLeuAsp99510001005ArgLeuLysSerGluGlyIleLeuAspHisValAlaLeuValTyrGly101010151020TyrPheProAlaValAlaGluGlyAspAspValValIleLeuGluSer1025103010351040ProAspProHisAlaAlaGluArgMetArgPheSerPheProArgGln104510501055GlnArgGlyArgPheLeuCysIleAlaAspPheIleArgProArgGlu106010651070GlnAlaValLysAspGlyGlnValAspValMetProPheGlnLeuVal107510801085ThrMetGlyAsnProIleAlaAspPheAlaAsnGluLeuPheAlaAla109010951100AsnGluTyrArgGluTyrLeuGluValHisGlyIleGlyValGlnLeu1105111011151120ThrGluAlaLeuAlaGluTyrTrpHisSerArgValArgSerGluLeu112511301135LysLeuAsnAspGlyGlySerValAlaAspPheAspProGluAspLys114011451150ThrLysPhePheAspLeuAspTyrArgGlyAlaArgPheSerPheGly115511601165TyrGlySerCysProAspLeuGluAspArgAlaLysLeuValGluLeu117011751180LeuGluProGlyArgIleGlyValGluLeuSerGluGluLeuGlnLeu1185119011951200HisProGluGlnSerThrAspAlaPheValLeuTyrHisProGluAla120512101215LysTyrPheAsnVal1220<210>91<211>1758<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1755)<223>RXA00098E<400>91atggccacgtcgaaaagcagcccaataaacgcacctaaatttgtcgtg48MetAlaThrSerLysSerSerProIleAsnAlaProLysPheValVal151015tttcccactttgaacactcttcgatgcgcttggccacaaaagcaagct96PheProThrLeuAsnThrLeuArgCysAlaTrpProGlnLysGlnAla202530aacctgaagatgttatttaacgacaataaaggagttttcatggcggac144AsnLeuLysMetLeuPheAsnAspAsnLysGlyValPheMetAlaAsp354045atttcgaccacccaggtttggcaagacctgaccgatcattactcaaac192IleSerThrThrGlnValTrpGlnAspLeuThrAspHisTyrSerAsn505560ttccaggcaaccactctgcgtgaacttttcaaggaagaaaaccgcgcc240PheGlnAlaThrThrLeuArgGluLeuPheLysGluGluAsnArgAla65707580gagaagtacaccttctccgcggctggcctccacgtcgacctgtcgaag288GluLysTyrThrPheSerAlaAlaGlyLeuHisValAspLeuSerLys859095aatctgcttgacgacgccaccctcaccaagctccttgcactgaccgaa336AsnLeuLeuAspAspAlaThrLeuThrLysLeuLeuAlaLeuThrGlu100105110gaatctggccttcgcgaacgcattgacgcgatgtttgccggtgaacac384GluSerGlyLeuArgGluArgIleAspAlaMetPheAlaGlyGluHis115120125ctcaacaacaccgaagaccgcgctgtcctccacaccgcgctgcgcctt432LeuAsnAsnThrGluAspArgAlaValLeuHisThrAlaLeuArgLeu130135140cctgccgaagctgatctgtcagtagatggccaagatgttgctgctgat480ProAlaGluAlaAspLeuSerValAspGlyGlnAspValAlaAlaAsp145150155160gtccacgaagttttgggacgcatgcgtgacttcgctactgcgctgcgc528ValHisGluValLeuGlyArgMetArgAspPheAlaThrAlaLeuArg165170175tcaggcaactggttgggacacaccggccacacgatcaagaagatcgtc576SerGlyAsnTrpLeuGlyHisThrGlyHisThrIleLysLysIleVal180185190aacattggtatcggtggctctgacctcggaccagccatggctacgaag624AsnIleGlyIleGlyGlySerAspLeuGlyProAlaMetAlaThrLys195200205gctctgcgtgcatacgcgaccgctggtatctcagcagaattcgtctcc672AlaLeuArgAlaTyrAlaThrAlaGlyIleSerAlaGluPheValSer210215220aacgtcgacccagcagacctcgtttctgtgttggaagacctcgatgca720AsnValAspProAlaAspLeuValSerValLeuGluAspLeuAspAla225230235240gaatccacattgttcgtgatcgcttcgaaaactttcaccacccaggag768GluSerThrLeuPheValIleAlaSerLysThrPheThrThrGlnGlu245250255acgctgtccaacgctcgtgcagctcgtgcttggctggtagagaagctc816ThrLeuSerAsnAlaArgAlaAlaArgAlaTrpLeuValGluLysLeu260265270ggtgaagaggctgtcgcgaagcacttcgtcgcagtgtccaccaatgct864GlyGluGluAlaValAlaLysHisPheValAlaValSerThrAsnAla275280285gaaaaggtcgcagagttcggtatcgacacggacaacatgttcggcttc912GluLysValAlaGluPheGlyIleAspThrAspAsnMetPheGlyPhe290295300tgggactgggtcggaggtcgttactccgtggactccgcagttggtctt960TrpAspTrpValGlyGlyArgTyrSerValAspSerAlaValGlyLeu305310315320tccctcatggcagtgatcggccctcgcgacttcatgcgtttcctcggt1008SerLeuMetAlaValIleGlyProArgAspPheMetArgPheLeuGly325330335ggattccacgcgatggatgaacacttccgcaccaccaagttcgaagag1056GlyPheHisAlaMetAspGluHisPheArgThrThrLysPheGluGlu340345350aacgttccaatcttgatggctctgctcggtgtctggtactccgatttc1104AsnValProIleLeuMetAlaLeuLeuGlyValTrpTyrSerAspPhe355360365tatggtgcagaaacccacgctgtcctaccttattccgaggatctcagc1152TyrGlyAlaGluThrHisAlaValLeuProTyrSerGluAspLeuSer370375380cgttttgctgcttacctccagcagctgaccatggaatcaaatggcaag1200ArgPheAlaAlaTyrLeuGlnGlnLeuThrMetGluSerAsnGlyLys385390395400tcagtccaccgcgacggctcccctgtttccactggcactggcgaaatt1248SerValHisArgAspGlySerProValSerThrGlyThrGlyGluIle405410415tactggggtgagcctggcacaaatggccagcacgctttcttccagctg1296TyrTrpGlyGluProGlyThrAsnGlyGlnHisAlaPhePheGlnLeu420425430atccaccagggcactcgccttgttccagctgatttcattggtttcgct1344IleHisGlnGlyThrArgLeuValProAlaAspPheIleGlyPheAla435440445cgtccaaagcaggatcttcctgccggtgagcgcaccatgcatgacctt1392ArgProLysGlnAspLeuProAlaGlyGluArgThrMetHisAspLeu450455460ttgatgagcaacttcttcgcacagaccaaggttttggctttcggtaag1440LeuMetSerAsnPhePheAlaGlnThrLysValLeuAlaPheGlyLys465470475480aacgctgaagagatcgctgcggaaggtgtcgcacctgagctggtcaac1488AsnAlaGluGluIleAlaAlaGluGlyValAlaProGluLeuValAsn485490495cacaaggtcatgccaggtaatcgcccaaccaccaccattttggcggag1536HisLysValMetProGlyAsnArgProThrThrThrIleLeuAlaGlu500505510gaacttaccccttctattctcggtgcgttgatcgctttgtacgaacac1584GluLeuThrProSerIleLeuGlyAlaLeuIleAlaLeuTyrGluHis515520525atcgtgatggttcagggcgtgatttgggacatcaaatccttcgaccaa1632IleValMetValGlnGlyValIleTrpAspIleAsnSerPheAspGln530535540tggggtgttgaactgggcaaacagcaggcaaatgacctcgctccggct1680TrpGlyValGluLeuGlyLysGlnGlnAlaAsnAspLeuAlaProAla545550555560gtctctggtgaagaggatgttgactcgggagattcttccactgattca1728ValSerGlyGluGluAspValAspSerGlyAspSerSerThrAspSer565570575ctgattaagtggtaccgcgcaaataggtag1758LeuIleLysTrpTyrArgAlaAsnArg580585<210>92<211>585<212>PRT<213>谷氨酸棒杆菌<400>92MetAlaThrSerLysSerSerProIleAsnAlaProLysPheValVal151015PheProThrLeuAsnThrLeuArgCysAlaTrpProGlnLysGlnAla202530AsnLeuLysMetLeuPheAsnAspAsnLysGlyValPheMetAlaAsp354045IleSerThrThrGlnValTrpGlnAspLeuThrAspHisTyrSerAsn505560PheGlnAlaThrThrLeuArgGluLeuPheLysGluGluAsnArgAla65707580GluLysTyrThrPheSerAlaAlaGlyLeuHisValAspLeuSerLys859095AsnLeuLeuAspAspAlaThrLeuThrLysLeuLeuAlaLeuThrGlu100105110GluSerGlyLeuArgGluArgIleAspAlaMetPheAlaGlyGluHis115120125LeuAsnAsnThrGluAspArgAlaValLeuHisThrAlaLeuArgLeu130135140ProAlaGluAlaAspLeuSerValAspGlyGlnAspValAlaAlaAsp145150155160ValHisGluValLeuGlyArgMetArgAspPheAlaThrAlaLeuArg165170175SerGlyAsnTrpLeuGlyHisThrGlyHisThrIleLysLysIleVal180185190AsnIleGlyIleGlyGlySerAspLeuGlyProAlaMetAlaThrLys195200205AlaLeuArgAlaTyrAlaThrAlaGlyIleSerAlaGluPheValSer210215220AsnValAspProAlaAspLeuValSerValLeuGluAspLeuAspAla225230235240GluSerThrLeuPheValIleAlaSerLysThrPheThrThrGlnGlu245250255ThrLeuSerAsnAlaArgAlaAlaArgAlaTrpLeuValGluLysLeu260265270GlyGluGluAlaValAlaLysHisPheValAlaValSerThrAsnAla275280285GluLysValAlaGluPheGlyIleAspThrAspAsnMetPheGlyPhe290295300TrpAspTrpValGlyGlyArgTyrSerValAspSerAlaValGlyLeu305310315320SerLeuMetAlaValIleGlyProArgAspPheMetArgPheLeuGly325330335GlyPheHisAlaMetAspGluHisPheArgThrThrLysPheGluGlu340345350AsnValProIleLeuMetAlaLeuLeuGlyValTrpTyrSerAspPhe355360365TyrGlyAlaGluThrHisAlaValLeuProTyrSerGluAspLeuSer370375380ArgPheAlaAlaTyrLeuGlnGlnLeuThrMetGluSerAsnGlyLys385390395400SerValHisArgAspGlySerProValSerThrGlyThrGlyGluIle405410415TyrTrpGlyGluProGlyThrAsnGlyGlnHisAlaPhePheGlnLeu420425430IleHisGlnGlyThrArgLeuValProAlaAspPheIleGlyPheAla435440445ArgProLysGlnAspLeuProAlaGlyGluArgThrMetHisAspLeu450455460LeuMetSerAsnPhePheAlaGlnThrLysValLeuAlaPheGlyLys465470475480AsnAlaGlaGluIleAlaAlaGluGlyValAlaProGluLeuValAsn485490495HisLysValMerProGlyAsnArgProThrThrThrIleLeuAlaGlu500505510GluLeuThrProSerIleLeuGlyAlaLeuIleAlaLeuTyrGluHis515520525IleValMetValGlnGlyValIleTrpAspIleAsnSerPheAspGln530535540TrpGlyValGluLeuGlyLysGlnGlnAlaAsnAspLeualaProAla545550555560ValSerGlyGluGluAspValAspSerGlyAspSerSerThrAspSer565570575LeuIleLysTrpTyrArgAlaAsnArg580585<210>93<211>1503<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1503)<223>RXA00241K<400>93gtgaatactcaatcagattctgcggggtctcaaggtgcagcggccaca48MetAsnThrGlnSerAspSerAlaGlySerGlnGlyAlaAlaAlaThr151015agtcgtactgtatctattagaaccctcatcgcgctgatcatcggatcg96SerArgThrValSerIleArgThrLeuIleAlaLeuIleIleGlySer202530accgtcggcgcgggaattttctccatccctcaaaacatcggctcagtc144ThrValGlyAlaGlyIlePheSerIleProGlnAsnIleGlySerVal354045gcaggtcccggcgcgatgctcatcggctggctgatcgccggtgtgggc192AlaGlyProGlyAlaMetLeuIleGlyTrpLeuIleAlaGlyValGly505560atgttgtccgtagcgttcgtgttccatgttcttgcccgccgtaaacct240MetLeuSerValAlaPheValPheHisValLeuAlaArgArgLysPro65707580cacctcgattctggcgtctacgcatatgcgcgtgttggattgggcgat288HisLeuAspSerGlyValTyrAlaTyrAlaArgValGlyLeuGlyAsp859095tatgtaggtttctcctccgcttggggttattggctgggttcagtcatc336TyrValGlyPheSerSerAlaTrpGlyTyrTrpLeuGlySerValIle100105110gcccaagttggctacgcaacgttatttttctccacgttgggccactac384AlaGlnValGlyTyrAlaThrLeuPhePheSerThrLeuGlyHisTyr115120125gtaccgctgttttcccaagatcatccatttgtgtcagcgttggcagtt432ValProLeuPheSerGlnAspHisProPheValSerAlaLeuAlaVal130135140agcgctttgacctggctggtgtttggagttgtttcccgaggaattagc480SerAlaLeuThrTrpLeuValPheGlyValValSerArgGlyIleSer145150155160caagctgctttcttgacaacggtcaccaccgtggccaaaattctgcct528GlnAlaAlaPheLeuThrThrValThrThrValAlaLysIleLeuPro165170175ctgttgtgcttcatcatccttgttgcattcttgggctttagctgggag576LeuLeuCysPheIleIleLeuValAlaPheLeuGlyPheSerTrpGlu180185190aagttcactgttgatttatgggcgcgtgatggtggcgtgggcagcatt624LysPheThrValAspLeuTrpAlaArgAspGlyGlyValGlySerIle195200205tttgatcaggtgcgcggcatcatggtgtacaccgtgtgggtgttcatc672PheAspGlnValArgGlyIleMetValTyrThrValTrpValPheIle210215220ggtatcgaaggtgcatcggtatattcccgccaggcacgctcacgcagt720GlyIleGluGlyAlaSerValTyrSerArgGlnAlaArgSerArgSer225230235240gatgtcagccgagctaccgtgattggttttgtggctgttctccttttg768AspValSerArgAlaThrValIleGlyPheValAlaValLeuLeuLeu245250255ctggtgtcgatttcttcgctgagcttcggtgtactgacccaacaagag816LeuValSerIleSerSerLeuSerPheGlyValLeuThrGlnGlnGlu260265270ctcgctgcgttaccagataattccatggcgtcggtgctcgaagctgtt864LeuAlaAlaLeuProAspAsnSerMetAlaSerValLeuGluAlaVal275280285gttggtccatggggtgccgcattgatttcgttgggtctgtgtctttcg912ValGlyProTrpGlyAlaAlaLeuIleSerLeuGlyLeuCysLeuSer290295300gttcttggggcctatgtgtcctggcagatgctctgcgcagaaccactg960ValLeuGlyAlaTyrValSerTrpGlnMetLeuCysAlaGluProLeu305310315320gcgttgatggcaatggatggcctcattccaagcaaaatcggggccatc1008AlaLeuMetAlaMetAspGlyLeuIleProSerLysIleGlyAlaIle325330335aacagccgcggtgctgcctggatggctcagctgatctccaccatcgtg1056AsnSerArgGlyAlaAlaTrpMetAlaGlnLeuIleSerThrIleVal340345350attcagattttcatcatcattttcttcctcaacgagaccacctacgtc1104IleGlnIlePheIleIleIlePhePheLeuAsnGluThrThrTyrVal355360365tccatggtgcaattggctaccaacctatacttggtgccttacctgttc1152SerMetValGlnLeuAlaThrAsnLeuTyrLeuValProTyrLeuPhe370375380tctgccttttatctggtcatgctggcaacacgtggaaaaggaatcacc1200SerAlaPheTyrLeuValMetLeuAlaThrArgGlyLysGlyIleThr385390395400cacccacatgccggcacacgttttgatgattccggtccagagatatcc1248HisProHisAlaGlyThrArgPheAspAspSerGlyProGluIleSer405410415cgccgagaaaaccgcaaacacctcatcgtcggtttagtagcaacggtg1296ArgArgGluAsnArgLysHisLeuIleValGlyLeuValAlaThrVal420425430tattcagtgtggctgttttacgctgcagaaccgcagtttgtcctcttc1344TyrSerValTrpLeuPheTyrAlaAlaGluProGlnPheValLeuPhe435440445ggagccatggcgatgcttcccggcttaatcccctatgtgtggacaagg1392GlyAlaMetAlaMetLeuProGlyLeuIleProTyrValTrpThrArg450455460atttatcgtggcgaacaggtgtttaaccgctttgaaatcggcgtggtt1440IleTyrArgGlyGluGlnValPheAsnArgPheGluIleGlyValVal465470475480gttgtcctggtcgttgctgccagcgcgggcgttattggtttggtcaac1488ValValLeuValValAlaAlaSerAlaGlyValIleGlyLeuValAsn485490495ggatcactatcgctt1503GlySerLeuSerLeu500<210>94<211>501<212>PRT<213>谷氨酸棒杆菌<400>94MetAsnThrGlnSerAspSerAlaGlySerGlnGlyAlaAlaAlaThr151015SerArgThrValSerIleArgThrLeuIleAlaLeuIleIleGlySer202530ThrValGlyAlaGlyIlePheSerIleProGlnAsnIleGlySerVal354045AlaGlyProGlyAlaMetLeuIleGlyTrpLeuIleAlaGlyValGly505560MetLeuSerValAlaPheValPheHisValLeuAlaArgArgLysPro65707580HisLeuAspSerGlyValTyrAlaTyrAlaArgValGlyLeuGlyAsp859095TyrValGlyPheSerSerAlaTrpGlyTyrTrpLeuGlySerValIle100105110AlaGlnValGlyTyrAlaThrLeuPhePheSerThrLeuGlyHisTyr115120125ValProLeuPheSerGlnAspHisProPheValSerAlaLeuAlaVal130135140SerAlaLeuThrTrpLeuValPheGlyValValSerArgGlyIleSer145150155160GlnAlaAlaPheLeuThrThrValThrThrValAlaLysIleLeuPro165170175LeuLeuCysPheIleIleLeuValAlaPheLeuGlyPheSerTrpGlu180185190LysPheThrValAspLeuTrpAlaArgAspGlyGlyValGlySerIle195200205PheAspGlnValArgGlyIleMetValTyrThrValTrpValPheIle210215220GlyIleGluGlyAlaSerValTyrSerArgGlnAlaArgSerArgSer225230235240AspValSerArgAlaThrValIleGlyPheValAlaValLeuLeuLeu245250255LeuValSerIleSerSerLeuSerPheGlyValLeuThrGlnGlnGlu260265270LeuAlaAlaLeuProAspAsnSerMetAlaSerValLeuGluAlaVal275280285ValGlyProTrpGlyAlaAlaLeuIleSerLeuGlyLeuCysLeuSer290295300ValLeuGlyAlaTyrValSerTrpGlnMetLeuCysAlaGluProLeu305310315320AlaLeuMetAlaMetAspGlyLeuIleProSerLysIleGlyAlaIle325330335AsnSerArgGlyAlaAlaTrpMetAlaGlnLeuIleSerThrIleVal340345350IleGlnIlePheIleIleIlePhePheLeuAsnGluThrThrTyrVal355360365SerMetValGlnLeuAlaThrAsnLeuTyrLeuValProTyrLeuPhe370375380SerAlaPheTyrLeuValMetLeuAlaThrArgGlyLysGlyIleThr385390395400HisProHisAlaGlyThrArgPheAspAspSerGlyProGluIleSer405410415ArgArgGluAsnArgLysHisLeuIleValGlyLeuValAlaThrVal420425430TyrSerValTrpLeuPheTyrAlaAlaGluProGlnPheValLeuPhe435440445GlyAlaMetAlaMetLeuProGlyLeuIleProTyrValTrpThrArg450455460IleTyrArgGlyGluGlnValPheAsnArgPheGluIleGlyValVal465470475480ValValLeuValValAlaAlaSerAlaGlyValIleGlyLeuValAsn485490495GlySerLeuSerLeu500<210>95<211>1086<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1086)<223>RXA00517E<400>95ttgctggtatacatttcggtatacctaaaccgaattgagggattcatg48MetLeuValTyrIleSerValTyrLeuAsnArgIleGluGlyPheMet151015ccagaagtcactgtcaacgcccaacaactcactgttctctgcacagac96ProGluValThrValAsnAlaGlnGlnLeuThrValLeuCysThrAsp202530atcctcaccaaaactggagtacctgcagcagacgcccatcttgtcggt144IleLeuThrLysThrGlyValProAlaAlaAspAlaHisLeuValGly354045gatagtttggtgcaggctgatctttggggtcacccctcccacggtgtg192AspSerLeuValGlnAlaAspLeuTrpGlyHisProSerHisGlyVal505560cttcgactgccttggtatgtgcgcagactccacagtggcgcgatgact240LeuArgLeuProTrpTyrValArgArgLeuHisSerGlyAlaMetThr65707580acacatgcacacgtggaggttctcaatgatttgggtgccgtgttggcg288ThrHisAlaHisValGluValLeuAsnAspLeuGlyAlaValLeuAla859095ttggatggacacaatggaatcggccaagttttagctgatcatgctcgt336LeuAspGlyHisAsnGlyIleGlyGlnValLeuAlaAspHisAlaArg100105110aaagaagcagtgactagggcaatgatgttcggcatcggtgcggtgtcg384LysGluAlaValThrArgAlaMetMetPheGlyIleGlyAlaValSer115120125gtgcgcaactccaatcattttggaactgccatgtactacacccggaaa432ValArgAsnSerAsnHisPheGlyThrAlaMetTyrTyrThrArgLys130135140gcggcagcgcaaggatgtgtttccattctcaccaccaatgcatctccg480AlaAlaAlaGlnGlyCysValSerIleLeuThrThrAsnAlaSerPro145150155160gcgatggcgccctggggtggcagagaaaaacggatcggtaccaaccca528AlaMetAlaProTrpGlyGlyArgGluLysArgIleGlyThrAsnPro165170175tggtctattgcggcaccttttggagaaacggctacggtagtcgatata576TrpSerIleAlaAlaProPheGlyGluThrAlaThrValValAspIle180185190gccaatactgcggttgcgcgcggaaagatctaccacgcacgacagaca624AlaAsnThrAlaValAlaArgGlyLysIleTyrHisAlaArgGlnThr195200205aacatgcccattcctgagacttgggcgatcacgagtgagggcgcaccc672AsnMetProIleProGluThrTrpAlaIleThrSerGluGlyAlaPro210215220accacggatcctgctgaggcaatcaacggtgtcgtccttcccatggct720ThrThrAspProAlaGluAlaIleAsnGlyValValLeuProMetAla225230235240ggtcacaaaggttatgcgattagcttcatgatggatgtgctttctgga768GlyHisLysGlyTyrAlaIleSerPheMetMetAspValLeuSerGly245250255gttctcactggttcccagcacagcaccaaggtacatggtccgtatgat816ValLeuThrGlySerGlnHisSerThrLysValHisGlyProTyrAsp260265270cccactcccccaggtggagctggccacttgttcatcgcgttggatgtt864ProThrProProGlyGlyAlaGlyHisLeuPheIleAlaLeuAspVal275280285gcagcgtttcgcgatccacaagatttcgatgacgcactcagcgatctg912AlaAlaPheArgAspProGlnAspPheAspAspAlaLeuSerAspLeu290295300gttggggaagttaaatccactccgaaagcacaaaacaccgaggagatt960ValGlyGluValLysSerThrProLysAlaGlnAsnThrGluGluIle305310315320ttctaccccggtgaatcggaagaccgtgcgcatcggaaaaactctgcg1008PheTyrProGlyGluSerGluAspArgAlaHisArgLysAsnSerAla325330335cacggtatttcattgcctgaaaaaacgtggatggaactgcaagaactg1056HisGlyIleSerLeuProGluLysThrTrpMetGluLeuGlnGluLeu340345350gcaatcgagaaccatgttgtaactcaccgt1086AlaIleGluAsnHisValValThrHisArg355360<210>96<211>362<212>PRT<213>谷氨酸棒杆菌<400>96MetLeuValTyrIleSerValTyrLeuAsnArgIleGluGlyPheMet151015ProGluValThrValAsnAlaGlnGlnLeuThrValLeuCysThrAsp202530IleLeuThrLysThrGlyValProAlaAlaAspAlaHisLeuValGly354045AspSerLeuValGlnAlaAspLeuTrpGlyHisProSerHisGlyVal505560LeuArgLeuProTrpTyrValArgArgLeuHisSerGlyAlaMetThr65707580ThrHisAlaHisValGluValLeuAsnAspLeuGlyAlaValLeuAla859095LeuAspGlyHisAsnGlyIleGlyGlnValLeuAlaAspHisAlaArg100105110LysGluAlaValThrArgAlaMetMetPheGlyIleGlyAlaValSer115120125ValArgAsnSerAsnHisPheGlyThrAlaMetTyrTyrThrArgLys130135140AlaAlaAlaGlnGlyCysValSerIleLeuThrThrAsnAlaSerPro145150155160AlaMetAlaProTrpGlyGlyArgGluLysArgIleGlyThrAsnPro165170175TrpSerIleAlaAlaProPheGlyGluThrAlaThrValValAspIle180185190AlaAsnThrAlaValAlaArgGlyLysIleTyrHisAlaArgGlnThr195200205AsnMetProIleProGluThrTrpAlaIleThrSerGluGlyAlaPro210215220ThrThrAspProAlaGluAlaIleAsnGlyValValLeuProMetAla225230235240GlyHisLysGlyTyrAlaIleSerPheMetMetAspValLeuSerGly245250255ValLeuThrGlySerGlnHisSerThrLysValHisGlyProTyrAsp260265270ProThrProProGlyGlyAlaGlyHisLeuPheIleAlaLeuAspVal275280285AlaAlaPheArgAspProGlnAspPheAspAspAlaLeuSerAspLeu290295300ValGlyGluValLysSerThrProLysAlaGlnAsnThrGluGluIle305310315320PheTyrProGlyGluSerGluAspArgAlaHisArgLysAsnSerAla325330335HisGlyIleSerLeuProGluLysThrTrpMetGluLeuGlnGluLeu340345350AlaIleGluAsnHisValValThrHisArg355360<210>97<211>1041<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1041)<223>RXA00517W<400>97atgccagaagtcactgtcaacgcccaacaactcactgttctctgcaca48MetProGluValThrValAsnAlaGlnGlnLeuThrValLeuCysThr151015gacatcctcaccaaaactggagtacctgcagcagacgcccatcttgtc96AspIleLeuThrLysThrGlyValProAlaAlaAspAlaHisLeuVal202530ggtgatagtttggtgcaggctgatctttggggtcacccctcccacggt144GlyAspSerLeuValGlnAlaAspLeuTrpGlyHisProSerHisGly354045gtgcttcgactgccttggtatgtgcgcagactccacagtggcgcgatg192ValLeuArgLeuProTrpTyrValArgArgLeuHisSerGlyAlaMet505560actacacatgcacacgtggaggttctcaatgatttgggtgccgtgttg240ThrThrHisAlaHisValGluValLeuAsnAspLeuGlyAlaValLeu65707580gcgttggatggacacaatggaatcggccaagttttagctgatcatgct288AlaLeuAspGlyHisAsnGlyIleGlyGlnValLeuAlaAspHisAla859095cgtaaagaagcagtgactagggcaatgatgttcggcatcggtgcggtg336ArgLysGluAlaValThrArgAlaMetMetPheGlyIleGlyAlaVal100105110tcggtgcgcaactccaatcattttggaactgccatgtactacacccgg384SerValArgAsnSerAsnHisPheGlyThrAlaMetTyrTyrThrArg115120125aaagcggcagcgcaaggatgtgtttccattctcaccaccaatgcatct432LysalaalaAlaGlnGlyCysValSerIleLeuThrThrAsnAlaSer130135140ccggcgatggcgccctggggtggcagagaaaaacggatcggtaccaac480ProAlaMetAlaProTrpGlyGlyArgGluLysArgIleGlyThrAsn145150155160ccatggtctattgcggcaccttttggagaaacggctacggtagtcgat528ProTrpSerIleAlaAlaProPheGlyGluThrAlaThrValValAsp165170175atagccaatactgcggttgcgcgcggaaagatctaccacgcacgacag576IleAlaAsnThrAlaValAlaArgGlyLysIleTyrHisAlaArgGla180185190acaaacatgcccattcctgagacttgggcgatcacgagtgagggcgca624ThrAsnMetProIleProGluThrTrpAlaIleThrSerGluGlyAla195200205cccaccacggatcctgctgaggcaatcaacggtgtcgtccttcccatg672ProThrThrAspProAlaGluAlaIleAsnGlyValValLeuProMet210215220gctggtcacaaaggttatgcgattagcttcatgatggatgtgctttct720AlaGlyHisLysGlyTyrAlaIleSerPheMetMetAspValLeuSer225230235240ggagttctcactggttcccagcacagcaccaaggtacatggtccgtat768GlyValLeuThrGlySerGlnHisSerThrLysValHisGlyProTyr245250255gatcccactcccccaggtggagctggccacttgttcatcgcgttggat816AspProThrProProGlyGlyAlaGlyHisLeuPheIleAlaLeuAsp260265270gttgcagcgtttcgcgatccacaagatttcgatgacgcactcagcgat864ValAlaAlaPheArgAspProGlnAspPheAspAspAlaLeuSerAsp275280285ctggttggggaagttaaatccactccgaaagcacaaaacaccgaggag912LeuValGlyGluValLysSerThrProLysAlaGlnAsnThrGluGlu290295300attttctaccccggtgaatcggaagaccgtgcgcatcggaaaaactct960IlePheTyrProGlyGluSerGluAspArgAlaHisArgLysAsnSer305310315320gcgcacggtatttcattgcctgaaaaaacgtggatggaactgcaagaa1008AlaHisGlyIleSerLeuProGluLysThrTrpMetGluLeuGlnGlu325330335ctggcaatcgagaaccatgttgtaactcaccgt1041LeuAlaIleGluAsnHisValValThrHisArg340345<210>98<211>347<212>PRT<213>谷氨酸棒杆菌<400>98MetProGluValThrValAsnAlaGlnGlnLeuThrValLeuCysThr151015AspIleLeuThrLysThrGlyValProAlaAlaAspAlaHisLeuVal202530GlyAspSerLeuValGlnAlaAspLeuTrpGlyHisProSerHisGly354045ValLeuArgLeuProTrpTyrValArgArgLeuHisSerGlyAlaMet505560ThrThrHisAlaHisValGluValLeuAsnAspLeuGlyAlaValLeu65707580AlaLeuAspGlyHisAsnGlyIleGlyGlnValLeuAlaAspHisAla859095ArgLysGluAlaValThrArgAlaMetMetPheGlyIleGlyAlaVal100105110SerValArgAsnSerAsnHisPheGlyThrAlaMetTyrTyrThrArg115120125LysAlaAlaAlaGlnGlyCysValSerIleLeuThrThrAsnAlaSer130135140ProAlaMetAlaProTrpGlyGlyArgGluLysArgIleGlyThrAsn145150155160ProTrpSerIleAlaAlaProPheGlyGluThrAlaThrValValAsp165170175IleAlaAsnThrAlaValAlaArgGlyLysIleTyrHisAlaArgGln180185190ThrAsnMetProIleProGluThrTrpAlaIleThrSerGluGlyAla195200205ProThrThrAspProAlaGluAlaIleAsnGlyValValLeuProMet210215220AlaGlyHisLysGlyTyrAlaIleSerPheMetMetAspValLeuSer225230235240GlyValLeuThrGlySerGlnHisSerThrLysValHisGlyProTyr245250255AspProThrProProGlyGlyAlaGlyHisLeuPheIleAlaLeuAsp260265270ValAlaAlaPheArgAspProGlnAspPheAspAspAlaLeuSerAsp275280285LeuValGlyGluValLysSerThrProLysAlaGlnAsnThrGluGlu290295300IlePheTyrProGlyGluSerGluAspArgAlaHisArgLysAsnSer305310315320AlaHisGlyIleSerLeuProGluLysThrTrpMetGluLeuGlnGlu325330335LeuAlaIleGluAsnHisValValThrHisArg340345<210>99<211>2214<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2214)<223>RXA00519W<400>99atggctaagatcatctggacccgcaccgacgaagcaccgctgctcgcg48MetAlaLysIleIleTrpThrArgThrAspGluAlaProLeuLeuAla151015acctactcgctgaagccggtcgtcgaggcatttgctgctaccgcgggc96ThrTyrSerLeuLysProValValGluAlaPheAlaAlaThrAlaGly202530attgaggtcgagacccgggacatttcactcgctggacgcatcctcgcc144IleGluValGluThrArgAspIleSerLeuAlaGlyArgIleLeuAla354045cagttcccagagcgcctcaccgaagatcagaaggtaggcaacgcactc192GlnPheProGluArgLeuThrGluAspGlnLysValGlyAsnAlaLeu505560gcagaactcggcgagcttgctaagactcctgaagcaaacatcattaag240AlaGluLeuGlyGluLeuAlaLysThrProGluAlaAsnIleIleLys65707580cttccaaacatctccgcttctgttccacagctcaaggctgctattaag288LeuProAsnIleSerAlaSerValProGlnLeuLysAlaAlaIleLys859095gaactgcaggaccagggctacgacatcccagaactgcctgataacgcc336GluLeuGlnAspGlnGlyTyrAspIleProGluLeuProAspAsnAla100105110accaccgacgaggaaaaagacatcctcgcacgctacaacgctgttaag384ThrThrAspGluGluLysAspIleLeuAlaArgTyrAsnAlaValLys115120125ggttccgctgtgaacccagtgctgcgtgaaggcaactctgaccgccgc432GlySerAlaValAsnProValLeuArgGluGlyAsnSerAspArgArg130135140gcaccaatcgctgtcaagaactttgttaagaagttcccacaccgcatg480AlaProIleAlaValLysAsnPheValLysLysPheProHisArgMet145150155160ggcgagtggtctgcagattccaagaccaacgttgcaaccatggatgca528GlyGluTrpSerAlaAspSerLysThrAsnValAlaThrMetAspAla165170175aacgacttccgccacaacgagaagtccatcatcctcgacgctgctgat576AsnAspPheArgHisAsnGluLysSerIleIleLeuAspAlaAlaAsp180185190gaagttcagatcaagcacatcgcagctgacggcaccgagaccatcctc624GluValGlnIleLysHisIleAlaAlaAspGlyThrGluThrIleLeu195200205aaggacagcctcaagcttcttgaaggcgaagttctagacggaaccgtt672LysAspSerLeuLysLeuLeuGluGlyGluValLeuAspGlyThrVal210215220ctgtccgcaaaggcactggacgcattccttctcgagcaggtcgctcgc720LeuSerAlaLysAlaLeuAspAlaPheLeuLeuGluGlnValAlaArg225230235240gcaaaggcagaaggtatcctcttctccgcacacctgaaggccaccatg768AlaLysAlaGluGlyIleLeuPheSerAlaHisLeuLysAlaThrMet245250255atgaaggtctccgacccaatcatcttcggccacgttgtgcgcgcttac816MetLysValSerAspProIleIlePheGlyHisValValArgAlaTyr260265270ttcgcagacgttttcgcacagtacggtgagcagctgctcgcagctggc864PheAlaAspValPheAlaGlnTyrGlyGluGlnLeuLeuAlaAlaGly275280285ctcaacggcgaaaacggcctcgctgcaatcctctccggcttggagtcc912LeuAsnGlyGluAsnGlyLeuAlaAlaIleLeuSerGlyLeuGluSer290295300ctggacaacggcgaagaaatcaaggctgcattcgagaagggcttggaa960LeuAspAsnGlyGluGluIleLysAlaAlaPheGluLysGlyLeuGlu305310315320gacggcccagacctggccatggttaactccgctcgcggcatcaccaac1008AspGlyProAspLeuAlaMetValAsnSerAlaArgGlyIleThrAsn325330335ctgcatgtcccttccgatgtcatcgtggacgcttccatgccagcaatg1056LeuHisValProSerAspValIleValAspAlaSerMetProAlaMet340345350attcgtacctccggccacatgtggaacaaagacgaccaggagcaggac1104IleArgThrSerGlyHisMetTrpAsnLysAspAspGlnGluGlnAsp355360365accctggcaatcatcccagactcctcctacgctggcgtctaccagacc1152ThrLeuAlaIleIleProAspSerSerTyrAlaGlyValTyrGlnThr370375380gttatcgaagactgccgcaagaacggcgcattcgatccaaccaccatg1200ValIleGluAspCysArgLysAsnGlyAlaPheAspProThrThrMet385390395400ggtaccgtccctaacgttggtctgatggctcagaaggctgaagagtac1248GlyThrValProAsnValGlyLeuMetAlaGlnLysAlaGluGluTyr405410415ggctcccatgacaagaccttccgcatcgaagcagacggtgtggttcag1296GlySerHisAspLysThrPheArgIleGluAlaAspGlyValValGln420425430gttgtttcctccaacggcgacgttctcatcgagcacgacgttgaggca1344ValValSerSerAsnGlyAspValLeuIleGluHisAspValGluAla435440445aatgacatctggcgtgcatgccaggtcaaggatgccccaatccaggat1392AsnAspIleTrpArgAlaCysGlnValLysAspAlaProIleGlnAsp450455460tgggtaaagcttgctgtcacccgctcccgtctctccggaatgcctgca1440TrpValLysLeuAlaValThrArgSerArgLeuSerGlyMetProAla465470475480gtgttctggttggatccagagcgcgcacacgaccgcaacctggcttcc1488ValPheTrpLeuAspProGluArgAlaHisAspArgAsnLeuAlaSer485490495ctcgttgagaagtacctggctgaccacgacaccgagggcctggacatc1536LeuValGluLysTyrLeuAlaAspHisAspThrGluGlyLeuAspIle500505510cagatcctctcccctgttgaggcaacccagctctccatcgaccgcatc1584GlnIleLeuSerProValGluAlaThrGlnLeuSerIleAspArgIle515520525cgccgtggcgaggacaccatctctgtcaccggtaacgttctgcgtgac1632ArgArgGlyGluAspThrIleSerValThrGlyAsnValLeuArgAsp530535540tacaacaccgacctcttcccaatcctggagctgggcacctctgcaaag1680TyrAsnThrAspLeuPheProIleLeuGluLeuGlyThrSerAlaLys545550555560atgctgtctgtcgttcctttgatggctggcggcggactgttcgagacc1728MetLeuSerValValProLeuMetAlaGlyGlyGlyLeuPheGluThr565570575ggtgctggtggatctgctcctaagcacgtccagcaggttcaggaagaa1776GlyAlaGlyGlySerAlaProLysHisValGlnGlnValGlnGluGlu580585590aaccacctgcgttgggattccctcggtgagttcctcgcactggctgag1824AsnHisLeuArgTrpAspSerLeuGlyGluPheLeuAlaLeuAlaGlu595600605tccttccgccacgagctcaacaacaacggcaacaccaaggccggcgtt1872SerPheArgHisGluLeuAsnAsnAsnGlyAsnThrLysAlaGlyVal610615620ctggctgacgctctggacaaggcaactgagaagctgctgaacgaagag1920LeuAlaAspAlaLeuAspLysAlaThrGluLysLeuLeuAsnGluGlu625630635640aagtccccatcccgcaaggttggcgagatcgacaaccgtggctcccac1968LysSerProSerArgLysValGlyGluIleAspAsnArgGlySerHis645650655ttctggctgaccaagttctgggctgacgagctcgctgctcagaccgag2016PheTrpLeuThrLysPheTrpAlaAspGluLeuAlaAlaGlnThrGlu660665670gacgcagatctggctgctaccttcgcaccagtcgcagaagcactgaac2064AspAlaAspLeuAlaAlaThrPheAlaProValAlaGluAlaLeuAsn675680685acaggcgctgcagacatcgatgctgcactgctcgcagttcagggtgga2112ThrGlyAlaAlaAspIleAspAlaAlaLeuLeuAlaValGlnGlyGly690695700gcaactgaccttggtggctactactcccctaacgaggagaagctcacc2160AlaThrAspLeuGlyGlyTyrTyrSerProAsnGluGluLysLeuThr705710715720aacatcatgcgcccagtcgcacagttcaacgagatcgttgacgcactg2208AsnIleMetArgProValAlaGlnPheAsnGluIleValAspAlaLeu725730735aagaag2214LysLys<210>100<211>738<212>PRT<213>谷氨酸棒杆菌<400>100MetAlaLysIleIleTrpThrArgThrAspGluAlaProLeuLeuAla151015ThrTyrSerLeuLysProValValGluAlaPheAlaAlaThrAlaGly202530IleGluValGluThrArgAspIleSerLeuAlaGlyArgIleLeuAla354045GlnPheProGluArgLeuThrGluAspGlnLysValGlyAsnAlaLeu505560AlaGluLeuGlyGluLeuAlaLysThrProGluAlaAsnIleIleLys65707580LeuProAsnIleSerAlaSerValProGlnLeuLysAlaAlaIleLys859095GluLeuGlnAspGlnGlyTyrAspIleProGluLeuProAspAsnAla100105110ThrThrAspGluGluLysAspIleLeuAlaArgTyrAsnAlaValLys115120125GlySerAlaValAsnProValLeuArgGluGlyAsnSerAspArgArg130135140AlaProIleAlaValLysAsnPheValLysLysPheProHisArgMet145150155160GlyGluTrpSerAlaAspSerLysThrAsnValAlaThrMetAspAla165170175AsnAspPheArgHisAsnGluLysSerIleIleLeuAspAlaAlaAsp180185190GluValGlnIleLysHisIleAlaAlaAspGlyThrGluThrIleLeu195200205LysAspSerLeuLysLeuLeuGluGlyGluValLeuAspGlyThrVal210215220LeuSerAlaLysAlaLeuAspAlaPheLeuLeuGluGlnValAlaArg225230235240AlaLysAlaGluGlyIleLeuPheSerAlaHisLeuLysAlaThrMet245250255MetLysValSerAspProIleIlePheGlyHisValValArgAlaTyr260265270PheAlaAspValPheAlaGlnTyrGlyGluGlnLeuLeuAlaAlaGly275280285LeuAsnGlyGluAsnGlyLeuAlaAlaIleLeuSerGlyLeuGluSer290295300LeuAspAsnGlyGluGluIleLysAlaAlaPheGluLysGlyLeuGlu305310315320AspGlyProAspLeuAlaMetValAsnSerAlaArgGlyIleThrAsn325330335LeuHisValProSerAspValIleValAspAlaSerMetProAlaMet340345350IleArgThrSerGlyHisMetTrpAsnLysAspAspGlnGluGlnAsp355360365ThrLeuAlaIleIleProAspSerSerTyrAlaGlyValTyrGlnThr370375380ValIleGluAspCysArgLysAsnGlyAlaPheAspProThrThrMet385390395400GlyThrValProAsnValGlyLeuMetAlaGlnLysAlaGluGluTyr405410415GlySerHisAspLysThrPheArgIleGluAlaAspGlyValValGln420425430ValValSerSerAsnGlyAspValLeuIleGluHisAspValGluAla435440445AsnAspIleTrpArgAlaCysGlnValLysAspAlaProIleGlnAsp450455460TrpValLysLeuAlaValThrArgSerArgLeuSerGlyMetProAla465470475480ValPheTrpLeuAspProGluArgAlaHisAspArgAsnLeuAlaSer485490495LeuValGluLysTyrLeuAlaAspHisAspThrGluGlyLeuAspIle500505510GlnIleLeuSerProValGluAlaThrGlnLeuSerIleAspArgIle515520525ArgArgGlyGluAspThrIleSerValThrGlyAsnValLeuArgAsp530535540TyrAsnThrAspLeuPheProIleLeuGluLeuGlyThrSerAlaLys545550555560MetLeuSerValValProLeuMetAlaGlyGlyGlyLeuPheGluThr565570575GlyAlaGlyGlySerAlaProLysHisValGlnGlnValGlnGluGlu580585590AsnHisLeuArgTrpAspSerLeuGlyGluPheLeuAlaLeuAlaGlu595600605SerPheArgHisGluLeuAsnAsnAsnGlyAsnThrLysAlaGlyVal610615620LeuAlaAspAlaLeuAspLysAlaThrGluLysLeuLeuAsnGluGlu625630635640LysSerProSerArgLysValGlyGluIleAspAsnArgGlySerHis645650655PheTrpLeuThrLysPheTrpAlaAspGluLeuAlaAlaGlnThrGlu660665670AspAlaAspLeuAlaAlaThrPheAlaProValAlaGluAlaLeuAsn675680685ThrGlyAlaAlaAspIleAspAlaAlaLeuLeuAlaValGlnGlyGly690695700AlaThrAspLeuGlyGlyTyrTyrSerProAsnGluGluLysLeuThr705710715720AsnIleMetArgProValAlaGlnPheAsnGluIleValAspAlaLeu725730735LysLys<210>101<211>984<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(984)<223>RXA00533E<400>101atgcgcacccttttggaagagcgcaatttcccagctgacactgttcgt48MetArgThrLeuLeuGluGluArgAsnPheProAlaAspThrValArg151015ttctttgcttccccacgttccgcaggccgtaagattgaattccgtggc96PhePheAlaSerProArgSerAlaGlyArgLysIleGluPheArgGly202530acggaaatcgaggtagaagacattactcaggcaaccgaggagtccctc144ThrGluIleGluValGluAspIleThrGlnAlaThrGluGluSerLeu354045aaggacatcgacgttgcgttgttctccgctggaggcaccgcttccaag192LysAspIleAspValAlaLeuPheSerAlaGlyGlyThrAlaSerLys505560cagtacgctccactgttcgctgctgcaggcgcgactgttgtggataac240GlnTyrAlaProLeuPheAlaAlaAlaGlyAlaThrValValAspAsn65707580tcttctgcttggcgcaaggacgacgaggttccactaatcgtctctgag288SerSerAlaTrpArgLysAspAspGluValProLeuIleValSerGlu859095gtgaacccttccgacaaggattccctggtcaagggcattattgcgaac336ValAsnProSerAspLysAspSerLeuValLysGlyIleIleAlaAsn100105110cctaactgcaccaccatggctgcgatgccagtgctgaagccacttcac384ProAsnCysThrThrMetAlaAlaMetProValLeuLysProLeuHis115120125gatgccgctggtcttgtaaagcttcacgtttcctcttaccaggctgtt432AspAlaAlaGlyLeuValLysLeuHisValSerSerTyrGlnAlaVal130135140tccggttctggtcttgcaggtgtggaaaccttggcaaagcaggttgct480SerGlySerGlyLeuAlaGlyValGluThrLeuAlaLysGlnValAla145150155160gcagttggagaccacaacgttgagttcgtccatgatggacaggctgct528AlaValGlyAspHisAsnValGluPheValHisAspGlyGlnAlaAla165170175gacgcaggcgatgtcggaccttatgtttcaccaatcgcttacaacgtg576AspAlaGlyAspValGlyProTyrValSerProIleAlaTyrAsnVal180185190ctgccattcgccggaaacctcgtcgatgacggcaccttcgaaaccgat624LeuProPheAlaGlyAsnLeuValAspAspGlyThrPheGluThrAsp195200205gaagagcagaagctgcgcaacgaatcccgcaagattctcggtctccca672GluGluGlnLysLeuArgAsnGluSerArgLysIleLeuGlyLeuPro210215220gacctcaaggtctcaggcacctgcgtccgcgtgccggttttcaccggc720AspLeuLysValSerGlyThrCysValArgValProValPheThrGly225230235240cacacgctgaccattcacgccgaattcgacaaggcaatcaccgtggac768HisThrLeuThrIleHisAlaGluPheAspLysAlaIleThrValAsp245250255caggcgcaggagatcttgggtgccgcttcaggcgtcaagcttgtcgac816GlnAlaGlnGluIleLeuGlyAlaAlaSerGlyValLysLeuValAsp260265270gtcccaaccccacttgcagctgccggcattgacgaatccctcgttgga864ValProThrProLeuAlaAlaAlaGlyIleAspGluSerLeuValGly275280285cgcatccgtcaggactccactgtcgacgataaccgcggtctggttctc912ArgIleArgGlnAspSerThrValAspAspAsnArgGlyLeuValLeu290295300gtcgtatctggcgacaacctccgcaagggtgctgcgctaaacaccatc960ValValSerGlyAspAsnLeuArgLysGlyAlaAlaLeuAsnThrIle305310315320cagatcgctgagctgctggttaag984GlnIleAlaGluLeuLeuValLys325<210>102<211>328<212>PRT<213>谷氨酸棒杆菌<400>102MetArgThrLeuLeuGluGluArgAsnPheProAlaAspThrValArg151015PhePheAlaSerProArgSerAlaGlyArgLysIleGluPheArgGly202530ThrGluIleGluValGluAspIleThrGlnAlaThrGlualuSerLeu354045LysAspIleAspValAlaLeuPheSerAlaGlyGlyThrAlaSerLys505560GlnTyrAlaProLeuPheAlaAlaAlaGlyAlaThrValValAspAsn65707580SerSerAlaTrpArgLysAspAspGluValProLeuIleValSerGlu859095ValAsnProSerAspLysAspSerLeuValLysGlyIleIleAlaAsn100105110ProAsnCysThrThrMetAlaAlaMetProValLeuLysProLeuHis115120125AspAlaAlaGlyLeuValLysLeuHisValSerSerTyrGlnAlaVal130135140SerGlySerGlyLeuAlaGlyValGluThrLeuAlaLysGlnValAla145150155160AlaValGlyAspHisAsnValGluPheValHisAspGlyGlnAlaAla165170175AspAlaGlyAspValGlyProTyrValSerProIleAlaTyrAsnVal180185190LeuProPheAlaGlyAsnLeuValAspAspGlyThrPheGluThrAsp195200205GluGluGlnLysLeuArgAsnGluSerArgLysIleLeuGlyLeuPro210215220AspLeuLysValSerGlyThrCysValArgValProValPheThrGly225230235240HisThrLeuThrIleHisAlaGluPheAspLysAlaIleThrValAsp245250255GlnAlaGlnGluIleLeuGlyAlaAlaSerGlyValLysLeuValAsp260265270ValProThrProLeuAlaAlaAlaGlyIleAspGluSerLeuValGly275280285ArgIleArgGlnAspSerThrValAspAspAsnArgGlyLeuValLeu290295300ValValSerGlyAspAsnLeuArgLysGlyAlaAlaLeuAsnThrIle305310315320GlnIleAlaGluLeuLeuValLys325<210>103<211>1032<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1032)<223>RXA00533K<400>103atgaccaccatcgcagttgttggtgcaaccggccaggtcggccaggtt48MetThrThrIleAlaValValGlyAlaThrGlyGlnValGlyGlnVal151015atgcgcacccttttggaagagcgcaatttcccagctgacactgttcgt96MetArgThrLeuLeuGluGluArgAsnPheProAlaAspThrValArg202530ttctttgcttccccacgttccgcaggccgtaagattgaattccgtggc144PhePheAlaSerProArgSerAlaGlyArgLysIleGluPheArgGly354045acggaaatcgaggtagaagacattactcaggcaaccgaggagtccctc192ThrGluIleGluValGluAspIleThrGlnAlaThrGluGluSerLeu505560aaggacatcgacgttgcgttgttctccgctggaggcaccgcttccaag240LysAspIleAspValAlaLeuPheSerAlaGlyGlyThrAlaSerLys65707580cagtacgctccactgttcgctgctgcaggcgcgactgttgtggataac288GlnTyrAlaProLeuPheAlaAlaAlaGlyAlaThrValValAspAsn859095tcttctgcttggcgcaaggacgacgaggttccactaatcgtctctgag336SerSerAlaTrpArgLysAspAspGluValProLeuIleValSerGlu100105110gtgaacccttccgacaaggattccctggtcaagggcattattgcgaac384ValAsnProSerAspLysAspSerLeuValLysGlyIleIleAlaAsn115120125cctaactgcaccaccatggctgcgatgccagtgctgaagccacttcac432ProAsnCysThrThrMetAlaAlaMetProValLeuLysProLeuHis130135140gatgccgctggtcttgtaaagcttcacgtttcctcttaccaggctgtt480AspAlaAlaGlyLeuValLysLeuHisValSerSerTyrGlnAlaVal145150155160tccggttctggtcttgcaggtgtggaaaccttggcaaagcaggttgct528SerGlySerGlyLeuAlaGlyValGluThrLeuAlaLysGlnValAla165170175gcagttggagaccacaacgttgagttcgtccatgatggacaggctgct576AlaValGlyAspHisAsnValGluPheValHisAspGlyGlnAlaAla180185190gacgcaggcgatgtcggaccttatgtttcaccaatcgcttacaacgtg624AspAlaGlyAspValGlyProTyrValSerProIleAlaTyrAsnVal195200205ctgccattcgccggaaacctcgtcgatgacggcaccttcgaaaccgat672LeuProPheAlaGlyAsnLeuValAspAspGlyThrPheGluThrAsp210215220gaagagcagaagctgcgcaacgaatcccgcaagattctcggtctccca720GluGluGlnLysLeuArgAsnGluSerArgLysIleLeuGlyLeuPro225230235240gacctcaaggtctcaggcacctgcgtccgcgtgccggttttcaccggc768AspLeuLysValSerGlyThrCysValArgValProValPheThrGly245250255cacacgctgaccattcacgccgaattcgacaaggcaatcaccgtggac816HisThrLeuThrIleHisAlaGluPheAspLysAlaIleThrValAsp260265270caggcgcaggagatcttgggtgccgcttcaggcgtcaagcttgtcgac864GlnAlaGlnGluIleLeuGlyAlaAlaSerGlyValLysLeuValAsp275280285gtcccaaccccacttgcagctgccggcattgacgaatccctcgttgga912ValProThrProLeuAlaAlaAlaGlyIleAspGluSerLeuValGly290295300cgcatccgtcaggactccactgtcgacgataaccgcggtctggttctc960ArgIleArgGlnAspSerThrValAspAspAsnArgGlyLeuValLeu305310315320gtcgtatctggcgacaacctccgcaagggtgctgcgctaaacaccatc1008ValValSerGlyAspAsnLeuArgLysGlyAlaAlaLeuAsnThrIle325330335cagatcgctgagctgctggttaag1032GlnIleAlaGluLeuLeuValLys340<210>104<211>344<212>PRT<213>谷氨酸棒杆菌<400>104MetThrThrIleAlaValValGlyAlaThrGlyGlnValGlyGlnVal151015MetArgThrLeuLeuGluGluArgAsnPheProAlaAspThrValArg202530PhePheAlaSerProArgSerAlaGlyArgLysIleGluPheArgGly354045ThrGluIleGluValGluAspIleThrGlnAlaThrGluGluSerLeu505560LysAspIleAspValAlaLeuPheSerAlaGlyGlyThrAlaSerLys65707580GlnTyrAlaProLeuPheAlaAlaAlaGlyAlaThrValValAspAsn859095SerSerAlaTrpArgLysAspAspGluValProLeuIleValSerGlu100105110ValAsnProSerAspLysAspSerLeuValLysGlyIleIleAlaAsn115120125ProAsnCysThrThrMetAlaAlaMetProValLeuLysProLeuHis130135140AspAlaAlaGlyLeuValLysLeuHisValSerSerTyrGlnAlaVal145150155160SerGlySerGlyLeuAlaGlyValGluThrLeuAlaLysGlnValAla165170175AlaValGlyAspHisAsnValGluPheValHisAspGlyGlnAlaAla180185190AspAlaGlyAspValGlyProTyrValSerProIleAlaTyrAsnVal195200205LeuProPheAlaGlyAsnLeuValAspAspGlyThrPheGluThrAsp210215220GluGluGlnLysLeuArgAsnGluSerArgLysIleLeuGlyLeuPro225230235240AspLeuLysValSerGlyThrCysValArgValProValPheThrGly245250255HisThrLeuThrIleHisAlaGluPheAspLysAlaIleThrValAsp260265270GlnAlaGlnGluIleLeuGlyAlaAlaSerGlyValLysLeuValAsp275280285ValProThrProLeuAlaAlaAlaGlyIleAspGluSerLeuValGly290295300ArgIleArgGlnAspSerThrValAspAspAsnArgGlyLeuValLeu305310315320ValValSerGlyAspAsnLeuArgLysGlyAlaAlaLeuAsnThrIle325330335GlnIleAlaGluLeuLeuValLys340<210>105<211>882<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(882)<223>RXA00782W<400>105atgtctatttttctcaattcagattcccgcatcatcattcagggcatt48MetSerIlePheLeuAsnSerAspSerArgIleIleIleGlnGlyIle151015accggttcggaaggttcagagcatgcgcgtcgaattttagcctctggt96ThrGlySerGluGlySerGluHisAlaArgArgIleLeuAlaSerGly202530gcgaagctcgtgggtggcaccaacccccgcaaagctgggcaaaccatt144AlaLysLeuValGlyGlyThrAsnProArgLysAlaGlyGlnThrIle354045ttgatcaatgacactgagttgcctgtatttggcactgttaaggaagca192LeuIleAsnAspThrGluLeuProValPheGlyThrValLysGluAla505560atggaggaaacgggtgcggatgtcaccgtaattttcgttcctccagcc240MetGluGluThrGlyAlaAspValThrValIlePheValProProAla65707580tttgccaaagctgcgatcattgaagctatcgacgctcacatcccactg288PheAlaLysAlaAlaIleIleGluAlaIleAspAlaHisIleProLeu859095tgcgtgattattactgagggcatcccagtgcgtgacgcttctgaggcg336CysValIleIleThrGluGlyIleProValArgAspAlaSerGluAla100105110tgggcttatgccaagaaggtgggacacacccgcatcattggccctaac384TrpAlaTyrAlaLysLysValGlyHisThrArgIleIleGlyProAsn115120125tgcccaggcattattactcccggcgaatctcttgcgggaattacgccg432CysProGlyIleIleThrProGlyGluSerLeuAlaGlyIleThrPro130135140gcaaacattgcaggttccggcccgatcgggttgatctcaaagtcggga480AlaAsaIleAlaGlySerGlyProIleGlyLeuIleSerLysSerGly145150155160acactgacttatcagatgatgtacgaactttcagatattggcatttct528ThrLeuThrTyrGlnMetMetTyrGluLeuSerAspIleGlyIleSer165170175acggcgattggtattggcggtgacccaatcatcggtacaacccatatc576ThrAlaIleGlyIleGlyGlyAspProIleIleGlyThrThrHisIle180185190gacgctctggaggcctttgaagctgatcctgagaccaaggcaatcgtc624AspAlaLeuGluAlaPheGluAlaAspProGluThrLysAlaIleVal195200205atgatcggtgagatcggtggagatgcagaggaacgcgctgctgacttc672MetIleGlyGluIleGlyGlyAspAlaGluGluArgAlaAlaAspPhe210215220atttctaagcacgtgacaaaaccagttgtgggttacgtggcaggcttt720IleSerLysHisValThrLysProValValGlyTyrValAlaGlyPhe225230235240accgcccctgaaggaaagaccatggggcatgctggcgccatcgtgaca768ThrAlaProGluGlyLysThrMetGlyHisAlaGlyAlaIleValThr245250255ggttcagaaggcactgcgcgagcaaagaagcatgcattggaggccgtg816GlySerGluGlyThrAlaArgAlaLysLysHisAlaLeuGluAlaVal260265270ggtgttcgcgtgggaacaactccgagtgaaaccgcgaagcttatgcgt864GlyValArgValGlyThrThrProSerGluThrAlaLysLeuMetArg275280285gaggtagttgcagctttg882GluValValAlaAlaLeu290<210>106<211>294<212>PRT<213>谷氨酸棒杆菌<400>106MetSerIlePheLeuAsnSerAspSerArgIleIleIleGlnGlyIle151015ThrGlySerGluGlySerGluHisAlaArgArgIleLeuAlaSerGly202530AlaLysLeuValGlyGlyThrAsnProArgLysAlaGlyGlnThrIle354045LeuIleAsnAspThrGluLeuProValPheGlyThrValLysGluAla505560MetGluGluThrGlyAlaAspValThrValIlePheValProProAla65707580PheAlaLysAlaAlaIleIleGluAlaIleAspAlaHisIleProLeu859095CysValIleIleThrGluGlyIleProValArgAspAlaSerGluAla100105110TrpAlaTyrAlaLysLysValGlyHisThrArgIleIleGlyProAsn115120125CysProGlyIleIleThrProGlyGluSerLeuAlaGlyIleThrPro130135140AlaAsnIleAlaGlySerGlyProIleGlyLeuIleSerLysSerGly145150155160ThrLeuThrTyrGlnMetMetTyrGluLeuSerAspIleGlyIleSer165170175ThrAlaIleGlyIleGlyGlyAspProIleIleGlyThrThrHisIle180185190AspAlaLeuGluAlaPheGluAlaAspProGluThrLysAlaIleVal195200205MetIleGlyGluIleGlyGlyAspAlaGluGluArgAlaAlaAspPhe210215220IleSerLysHisValThrLysProValValGlyTyrValAlaGlyPhe225230235240ThrAlaProGluGlyLysThrMetGlyHisAlaGlyAlaIleValThr245250255GlySerGluGlyThrAlaArgAlaLysLysHisAlaLeuGluAlaVal260265270GlyValArgValGlyThrThrProSerGluThrAlaLysLeuMetArg275280285GluValValAlaAlaLeu290<210>107<211>681<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(681)<223>RXA00822K<400>107gtggaaggtgtacaggagatcctgtcgcgcgccggaatttttcaaggc48MetGluGlyValGlnGluIleLeuSerArgAlaGlyIlePheGlnGly151015gttgacccaacggcagtcaataacctcatccaggatatggagaccgtt96ValAspProThrAlaValAsnAsnLeuIleGlnAspMetGluThrVal202530cgcttcccacgcggagcaaccatcttcgacgagggcgagccaggtgac144ArgPheProArgGlyAlaThrIlePheAspGluGlyGluProGlyAsp354045cgcctttacatcatcacctccggcaaagtgaagcttgcgcgccacgca192ArgLeuTyrIleIleThrSerGlyLysValLysLeuAlaArgHisAla505560ccggacggccgcgaaaacctgctgaccatcatgggtccttccgacatg240ProAspGlyArgGluAsnLeuLeuThrIleMetGlyProSerAspMet65707580ttcggtgagctctccatcttcgacccaggcccacgcacctcctctgca288PheGlyGluLeuSerIlePheAspProGlyProArgThrSerSerAla859095gtgtgtgtcaccgaagttcatgcagcaaccatgaactctgacatgctg336ValCysValThrGluValHisAlaAlaThrMetAsnSerAspMetLeu100105110cgcaactgggtagctgaccacccagctatcgctgagcagctcctgcgc384ArgAsnTrpValAlaAspHisProAlaIleAlaGluGlnLeuLeuArg115120125gttctggctcgtcgtctgcgtcgcaccaacgcttccctggctgacctc432ValLeuAlaArgArgLeuArgArgThrAsnAlaSerLeuAlaAspLeu130135140atcttcaccgacgtcccaggccgcgttgctaagacccttctgcagctg480IlePheThrAspValProGlyArgValAlaLysThrLeuLeuGlnLeu145150155160gctaaccgcttcggcacccaagaagctggcgcgctgcgcgtgaaccac528AlaAsnArgPheGlyThrGlnGluAlaGlyAlaLeuArgValAsnHis165170175gacctcactcaggaagaaatcgcacagctcgtcggtgcttcccgtgaa576AspLeuThrGlnGluGluIleAlaGlnLeuValGlyAlaSerArgGlu180185190actgtgaataaggctcttgcaacgttcgcacaccgtggctggattcgc624ThrValAsnLysAlaLeuAlaThrPheAlaHisArgGlyTrpIleArg195200205ctcgagggcaagtccgtcctcattgtggacaccgagcatttggcacgt672LeuGluGlyLysSerValLeuIleValAspThrGluHisLeuAlaArg210215220cgcgctcga681ArgAlaArg225<210>108<211>227<212>PRT<213>谷氨酸棒杆菌<400>108MetGluGlyValGlnGluIleLeuSerArgAlaGlyIlePheGlnaly151015ValAspProThrAlaValAsnAsnLeuIleGlnAspMetGluThrVal202530ArgPheProArgGlyAlaThrIlePheAspGluGlyGluProGlyAsp354045ArgLeuTyrIleIleThrSerGlyLysValLysLeuAlaArgHisAla505560ProAspGlyArgGluAsnLeuLeuThrIleMetGlyProSerAspMet65707580PheGlyGluLeuSerIlePheAspProGlyProArgThrSerSerAla859095ValCysValThrGluValHisAlaAlaThrMetAsnSerAspMetLeu100105110ArgAsnTrpValAlaAspHisProAlaIleAlaGluGlnLeuLeuArg115120125ValLeuAlaArgArgLeuArgArgThrAsnAlaSerLeuAlaAspLeu130135140IlePheThrAspValProGlyArgValAlaLysThrLeuLeuGlnLeu145150155160AlaAsnArgPheGlyThrGlnGluAlaGlyAlaLeuArgValAsnHis165170175AspLeuThrGlnGluGluIleAlaGlnLeuValGlyAlaSerArgGlu180185190ThrValAsnLysAlaLeuAlaThrPheAlaHisArgGlyTrpIleArg195200205LeuGluGlyLysSerValLeuIleValAspThrGluHisLeuAlaArg210215220ArgAlaArg225<210>109<211>1650<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1650)<223>RXA00975K<400>109atgacaccagctgatctcgcaacattgattaaagagaccgcggtagag48MetThrProAlaAspLeuAlaThrLeuIleLysGluThrAlaValGlu151015gttttgacctcccgcgagctcgatacttctgttcttccggagcaggta96ValLeuThrSerArgGluLeuAspThrSerValLeuProGluGlnVal202530gttgtggagcgtccgcgtaacccagagcacggcgattacgccaccaac144ValValGluArgProArgAsnProGluHisGlyAspTyrAlaThrAsn354045attgcattgcaggtggctaaaaaggtcggtcagaaccctcgggatttg192IleAlaLeuGlnValAlaLysLysValGlyGlnAsnProArgAspLeu505560gctacctggctggcagaggcattggctgcagatgacgccattgattct240AlaThrTrpLeuAlaGluAlaLeuAlaAlaAspAspAlaIleAspSer65707580gctgaaattgctggcccaggctttttgaacattcgccttgctgcagca288AlaGluIleAlaGlyProGlyPheLeuAsnIleArgLeuAlaAlaAla859095gcacagggtgaaattgtggccaagattctggcacagggcgagactttc336AlaGlnGlyGluIleValAlaLysIleLeuAlaGlnGlyGluThrPhe100105110ggaaactccgatcacctttcccacttggacgtgaacctcgagttcgtt384GlyAsnSerAspHisLeuSerHisLeuAspValAsnLeuGluPheVal115120125tctgcaaacccaaccggacctattcaccttggcggaacccgctgggct432SerAlaAsnProThrGlyProIleHisLeuGlyGlyThrArgTrpAla130135140gccgtgggtgactctttgggtcgtgtgctggaggcttccggcgcgaaa480AlaValGlyAspSerLeuGlyArgValLeuGluAlaSerGlyAlaLys145150155160gtgacccgcgaatactacttcaacgatcacggtcgccagatcgatcgt528ValThrArgGluTyrTyrPheAsnAspHisGlyArgGlnIleAspArg165170175ttcgctttgtcccttcttgcagcggcgaagggcgagccaacgccagaa576PheAlaLeuSerLeuLeuAlaAlaAlaLysGlyGluProThrProGlu180185190gacggttatggcggcgaatacattaaggaaattgcggaggcaatcgtc624AspGlyTyrGlyGlyGluTyrIleLysGluIleAlaGluAlaIleVal195200205gaaaagcatcctgaagcgttggctttggagcctgccgcaacccaggag672GluLysHisProGluAlaLeuAlaLeuGluProAlaAlaThrGlnGlu210215220cttttccgcgctgaaggcgtggagatgatgttcgagcacatcaaatct720LeuPheArgAlaGluGlyValGluMetMetPheGluHisIleLysSer225230235240tccctgcatgagttcggcaccgatttcgatgtctactaccacgagaac768SerLeuHisGluPheGlyThrAspPheAspValTyrTyrHisGluAsn245250255tccctgttcgagtccggtgcggtggacaaggccgtgcaggtgctgaag816SerLeuPheGluSerGlyAlaValAspLysAlaValGlnValLeuLys260265270gacaacggcaacctgtacgaaaacgagggcgcttggtggctgcgttcc864AspAsnGlyAsnLeuTyrGluAsnGluGlyAlaTrpTrpLeuArgSer275280285accgaattcggcgatgacaaagaccgcgtggtgatcaagtctgacggc912ThrGluPheGlyAspAspLysAspArgValValIleLysSerAspGly290295300gacgcagcctacatcgctggcgatatcgcgtacgtggctgataagttc960AspAlaAlaTyrIleAlaGlyAspIleAlaTyrValAlaAspLysPhe305310315320tcccgcggacacaacctaaacatctacatgttgggtgctgaccaccat1008SerArgGlyHisAsnLeuAsnIleTyrMetLeuGlyAlaAspHisHis325330335ggttacatcgcgcgcctgaaggcagcggcggcggcacttggctacaag1056GlyTyrIleAlaArgLeuLysAlaAlaAlaAlaAlaLeuGlyTyrLys340345350ccagaaggcgttgaagtcctgattggccagatggtgaacctgcttcgc1104ProGluGlyValGluValLeuIleGlyGlnMetValAsnLeuLeuArg355360365gacggcaaggcagtgcgtatgtccaagcgtgcaggcaccgtggtcacc1152AspGlyLysAlaValArgMetSerLysArgAlaGlyThrValValThr370375380ctagatgacctcgttgaagcaatcggcatcgatgcggcgcgttactcc1200LeuAspAspLeuValGluAlaIleGlyIleAspAlaAlaArgTyrSer385390395400ctgatccgttcctccgtggattcttccctggatatcgatctcggcctg1248LeuIleArgSerSerValAspSerSerLeuAspIleAspLeuGlyLeu405410415tgggaatcccagtcctccgacaaccctgtgtactacgtgcagtacgga1296TrpGluSerGlnSerSerAspAsnProValTyrTyrValGlnTyrGly420425430cacgctcgtctgtgctccatcgcgcgcaaggcagagaccttgggtgtc1344HisAlaArgLeuCysSerIleAlaArgLysAlaGluThrLeuGlyVal435440445accgaggaaggcgcagacctatctctactgacccacgaccgcgaaggc1392ThrGluGluGlyAlaAspLeuSerLeuLeuThrHisAspArgGluGly450455460gatctcatccgcacactcggagagttcccagcagtggtgaaggctgcc1440AspLeuIleArgThrLeuGlyGluPheProAlaValValLysAlaAla465470475480gctgacctacgtgaaccacaccgcattgcccgctatgctgaggaatta1488AlaAspLeuArgGluProHisArgIleAlaArgTyrAlaGluGluLeu485490495gctggaactttccaccgcttctacgattcctgccacatccttccaaag1536AlaGlyThrPheHisArgPheTyrAspSerCysHisIleLeuProLys500505510gttgatgaggatacggcaccaatccacacagcacgtctggcacttgca1584ValAspGluAspThrAlaProIleHisThrAlaArgLeuAlaLeuAla515520525gcagcaacccgccagaccctcgctaacgccctgcacctggttggcgtt1632AlaAlaThrArgGlnThrLeuAlaAsnAlaLeuHisLeuValGlyVal530535540tccgcaccggagaagatg1650SerAlaProGluLysMet545550<210>110<211>550<212>PRT<213>谷氨酸棒杆菌<400>110MetThrProAlaAspLeuAlaThrLeuIleLysGluThrAlaValGlu151015ValLeuThrSerArgGluLeuAspThrSerValLeuProGluGlnVal202530ValValGluArgProArgAsnProGluHisGlyAspTyrAlaThrAsn354045IleAlaLeuGlnValAlaLysLysValGlyGlnAsnProArgAspLeu505560AlaThrTrpLeuAlaGluAlaLeuAlaAlaAspAspAlaIleAspSer65707580AlaGluIleAlaGlyProGlyPheLeuAsnIleArgLeuAlaAlaAla859095AlaGlnGlyGluIleValAlaLysIleLeuAlaGlnGlyGluThrPhe100105110GlyAsnSerAspHisLeuSerHisLeuAspValAsnLeuGluPheVal115120125SerAlaAsnProThrGlyProIleHisLeuGlyGlyThrArgTrpAla130135140AlaValGlyAspSerLeuGlyArgValLeuGluAlaSerGlyAlaLys145150155160ValThrArgGluTyrTyrPheAsnAspHisGlyArgGlnIleAspArg165170175PheAlaLeuSerLeuLeuAlaAlaAlaLysGlyGluProThrProGlu180185190AspGlyTyrGlyGlyGluTyrIleLysGluIleAlaGluAlaIleVal195200205GluLysHisProGluAlaLeuAlaLeuGluProAlaAlaThrGlnGlu210215220LeuPheArgAlaGluGlyValGluMetMetPheGluHisIleLysSer225230235240SerLeuHisGluPheGlyThrAspPheAspValTyrTyrHisGluAsn245250255SerLeuPheGluSerGlyAlaValAspLysAlaValGlnValLeuLys260265270AspAsnGlyAsnLeuTyrGluAsnGluGlyAlaTrpTrpLeuArgSer275280285ThrGluPheGlyAspAspLysAspArgValValIleLysSerAspGly290295300AspAlaAlaTyrIleAlaGlyAspIleAlaTyrValAlaAspLysPhe305310315320SerArgGlyHisAsnLeuAsnIleTyrMetLeuGlyAlaAspHisHis325330335GlyTyrIleAlaArgLeuLysAlaAlaAlaAlaAlaLeuGlyTyrLys340345350ProGluGlyValGluValLeuIleGlyGlnMetValAsnLeuLeuArg355360365AspGlyLysAlaValArgMetSerLysArgAlaGlyThrValValThr370375380LeuAspAspLeuValGluAlaIleGlyIleAspAlaAlaArgTyrSer385390395400LeuIleArgSerSerValAspSerSerLeuAspIleAspLeuGlyLeu405410415TrpGluSerGlnSerSerAspAsnProValTyrTyrValGlnTyrGly420425430HisAlaArgLeuCysSerIleAlaArgLysAlaGluThrLeuGlyVal435440445ThraluGluGlyAlaAspLeuSerLeuLeuThrHisAspArgGluGly450455460AspLeuIleArgThrLeualyGluPheProAlaValValLysAlaAla465470475480AlaAspLeuArgGluProHisArgIleAlaArgTyrAlaGluGluLeu485490495AlaGlyThrPheHisArgPheTyrAspSerCysHisIleLeuProLys500505510ValAspGluAspThrAlaProIleHisThrAlaArgLeuAlaLeuAla515520525AlaAlaThrArgGlnThrLeuAlaAsnAlaLeuHisLeuValGlyVal530535540SerAlaProGluLysMet545550<210>111<211>750<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(747)<223>RXA01311E<400>111atgaaacttacacttgagatctggcgtcaagcaggcccaactgcggaa48MetLysLeuThrLeuGluIleTrpArgGlnAlaGlyProThrAlaGlu151015ggcaagttcgaaaccgtccaggttgacgacgccgtcgcgcagatgtcc96GlyLysPheGluThrValGlnValAspAspAlaValAlaGlnMetSer202530atcctggagctgcttgaccacgtaaacaacaagttcatcgaagaaggc144IleLeuGluLeuLeuAspHisValAsnAsnLysPheIleGluGluGly354045aaagaaccattcgcgttcgcctctgactgccgcgaaggcatttgtggt192LysGluProPheAlaPheAlaSerAspCysArgGluGlyIleCysGly505560acctgtggtctcctcgtgaacggtcgccctcacggcgccgaccagaac240ThrCysGlyLeuLeuValAsnGlyArgProHisGlyAlaAspGlnAsn65707580aagcctgcctgtgcgcagcgcctggtcagctacaaggaaggcgacacc288LysProAlaCysAlaGlnArgLeuValSerTyrLysGluGlyAspThr859095ctcaagatcgaaccactgcgttccgccgcatacccagtgatcaaggac336LeuLysIleGluProLeuArgSerAlaAlaTyrProValIleLysAsp100105110atggtcgtcgaccgctccgcactggaccgtgtcatggaacagggtggc384MetValValAspArgSerAlaLeuAspArgValMetGluGlnGlyGly115120125tacgtgaccatcaacgcaggtaccgcacctgacgctgataccctccac432TyrValThrIleAsnAlaGlyThrAlaProAspAlaAspThrLeuHis130135140gtcaaccacgaaaccgcagaactcgcacttgaccacgcagcctgcatc480ValAsnHisGluThrAlaGluLeuAlaLeuAspHisAlaAlaCysIle145150155160ggctgtggcgcatgtgttgctgcctgccctaacggcgcagcacacctg528GlyCysGlyAlaCysValAlaAlaCysProAsnGlyAlaAlaHisLeu165170175ttcaccggcgcaaagcttgttcacctctccctcctcccactgggtaag576PheThrGlyAlaLysLeuValHisLeuSerLeuLeuProLeuGlyLys180185190gaagagcgcggactgcgtgcacgtaagatggttgatgaaatggaaacc624GluGluArgGlyLeuArgAlaArgLysMetValAspGluMetGluThr195200205aacttcggacactgctccctctacggcgagtgcgcagatgtctgcccc672AsnPheGlyHisCysSerLeuTyrGlyGluCysAlaAspValCysPro210215220gcaggcatcccactgaccgctgtggcagctgtcaccaaggaacgtgcg720AlaGlyIleProLeuThrAlaValAlaAlaValThrLysGluArgAla225230235240cgtgcagctttccgaggcaaagacgactag750ArgAlaAlaPheArgGlyLysAspAsp245<210>112<211>249<212>PaT<213>谷氨酸棒杆菌<400>112MetLysLeuThrLeuGluIleTrpArgGlnAlaGlyProThrAlaGlu151015GlyLysPheGluThrValGlnValAspAspAlaValAlaGlnMetSer202530IleLeuGluLeuLeuAspHisValAsnAsnLysPheIleGluGluGly354045LysGluProPheAlaPheAlaSerAspCysArgGluGlyIleCysGly505560ThrCysGlyLeuLeuValAsnGlyArgProHisGlyAlaAspGlnAsn65707580LysProAlaCysAlaGlnArgLeuValSerTyrLysGluGlyAspThr859095LeuLysIleGluProLeuArgSerAlaAlaTyrProValIleLysAsp100105110MetValValAspArgSerAlaLeuAspArgValMetGluGlnGlyGly115120125TyrValThrIleAsnAlaGlyThrAlaProAspAlaAspThrLeuHis130135140ValAsnHisGluThrAlaGluLeuAlaLeuAspHisAlaAlaCysIle145150155160GlyCysGlyAlaCysValAlaAlaCysProAsnGlyAlaAlaHisLeu165170175PheThrGlyAlaLysLeuValHisLeuSerLeuLeuProLeuGlyLys180185190GluGluArgGlyLeuArgAlaArgLysMetValAspaluMetGluThr195200205AsnPheGlyHisCysSerLeuTyrGlyGluCysAlaAspValCysPro210215220AlaGlyIleProLeuThrAlaValAlaAlaValThrLysGluArgAla225230235240ArgAlaAlaPheArgGlyLysAspAsp245<210>113<211>837<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(837)<223>RXA01311K<400>113atgggaaccaggcgagaacggaaccttcgtccgccacgcagaaccact48MetGlyThrArgArgGluArgAsnLeuArgProProArgArgThrThr151015gttcttcgaatccgtcccactgcagacaaggaactacaagtaatgaaa96ValLeuArgIleArgProThrAlaAspLysGluLeuGlnValMetLys202530cttacacttgagatctggcgtcaagcaggcccaactgcggaaggcaag144LeuThrLeuGluIleTrpArgGlnAlaGlyProThrAlaGluGlyLys354045ttcgaaaccgtccaggttgacgacgccgtcgcgcagatgtccatcctg192PheGluThrValGlnValAspAspAlaValAlaGlnMetSerIleLeu505560gagctgcttgaccacgtaaacaacaagttcatcgaagaaggcaaagaa240GluLeuLeuAspHisValAsnAsnLysPheIleGluGluGlyLysGlu65707580ccattcgcgttcgcctctgactgccgcgaaggcatttgtggtacctgt288ProPheAlaPheAlaSerAspCysArgGluGlyIleCysGlyThrCys859095ggtctcctcgtgaacggtcgccctcacggcgccgaccagaacaagcct336GlyLeuLeuValAsnGlyArgProHisGlyAlaAspGlnAsnLysPro100105110gcctgtgcgcagcgcctggtcagctacaaggaaggcgacaccctcaag384AlaCysAlaGlnArgLeuValSerTyrLysGluGlyAspThrLeuLys115120125atcgaaccactgcgttccgccgcatacccagtgatcaaggacatggtc432IleGluProLeuArgSerAlaAlaTyrProValIleLysAspMetVal130135140gtcgaccgctccgcactggaccgtgtcatggaacagggtggctacgtg480ValAspArgSerAlaLeuAspArgValMetGluGlnGlyGlyTyrVal145150155160accatcaacgcaggtaccgcacctgacgctgataccctccacgtcaac528ThrIleAsnAlaGlyThrAlaProAspAlaAspThrLeuHisValAsn165170175cacgaaaccgcagaactcgcacttgaccacgcagcctgcatcggctgt576HisGluThrAlaGluLeuAlaLeuAspHisAlaAlaCysIleGlyCys180185190ggcgcatgtgttgctgcctgccctaacggcgcagcacacctgttcacc624GlyAlaCysValAlaAlaCysProAsnGlyAlaAlaHisLeuPheThr195200205ggcgcaaagcttgttcacctctccctcctcccactgggtaaggaagag672GlyAlaLysLeuValHisLeuSerLeuLeuProLeuGlyLysGluGlu210215220cgcggactgcgtgcacgtaagatggttgatgaaatggaaaccaacttc720ArgGlyLeuArgAlaArgLysMetValAspGluMetGluThrAsnPhe225230235240ggacactgctccctctacggcgagtgcgcagatgtctgccccgcaggc768GlyHisCysSerLeuTyrGlyGluCysAlaAspValCysProAlaGly245250255atcccactgaccgctgtggcagctgtcaccaaggaacgtgcgcgtgca816IleProLeuThrAlaValAlaAlaValThrLysGluArgAlaArgAla260265270gctttccgaggcaaagacgac837AlaPheArgGlyLysAspAsp275<210>114<211>279<212>PRT<213>谷氨酸棒杆菌<400>114MetGlyThrArgArgGluArgAsnLeuArgProProArgArgThrThr151015ValLeuArgIleArgProThrAlaAspLysGluLeuGlnValMetLys202530LeuThrLeuGluIleTrpArgGlnAlaGlyProThrAlaGluGlyLys354045PheGluThrValGlnValAspAspAlaValAlaGlnMetSerIleLeu505560GluLeuLeuAspHisValAsnAsnLysPheIleGluGluGlyLysGlu65707580ProPheAlaPheAlaSerAspCysArgGluGlyIleCysGlyThrCys859095GlyLeuLeuValAsnGlyArgProHisGlyAlaAspGlnAsnLysPro100105110AlaCysAlaGlnArgLeuValSerTyrLysGluGlyAspThrLeuLys115120125IleGluProLeuArgSerAlaAlaTyrProValIleLysAspMetVal130135140ValAspArgSerAlaLeuAspArgValMetGluGlnGlyGlyTyrVal145150155160ThrIleAsnAlaGlyThrAlaProAspAlaAspThrLeuHisValAsn165170175HisGluThrAlaGluLeuAlaLeuAspHisAlaAlaCysIleGlyCys180185190GlyAlaCysValAlaAlaCysProAsnGlyAlaAlaHisLeuPheThr195200205GlyAlaLysLeuValHisLeuSerLeuLeuProLeuGlyLysGluGlu210215220ArgGlyLeuArgAlaArgLysMetValAspGluMetGluThrAsnPhe225230235240GlyHisCysSerLeuTyrGlyGluCysAlaAspValCysProAlaGly245250255IleProLeuThrAlaValAlaAlaValThrLysGluArgAlaArgAla260265270AlaPheArgGlyLysAspAsp275<210>115<211>2019<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2019)<223>RXA01312E<400>115atgagcactcactctgaaaccacccgcccagagttcatccacccagtc48MetSerThrHisSerGluThrThrArgProGluPheIleHisProVal151015tcagtcctcccagaggtctcagctggtacggtccttgacgctgcagag96SerValLeuProGluValSerAlaGlyThrValLeuAspAlaAlaGlu202530ccagcaggcgttcccaccaaagatatgtgggaataccaaaaagaccac144ProAlaGlyValProThrLysAspMetTrpGluTyrGlnLysAspHis354045atgaacctggtctccccactgaaccgacgcaagttccgtgtcctcgtc192MetAsnLeuValSerProLeuAsnArgArgLysPheArgValLeuVal505560gttggcaccggcctgtccggtggtgctgcagcagcagccctcggcgaa240ValGlyThrGlyLeuSerGlyGlyAlaAlaAlaAlaAlaLeuGlyGlu65707580ctcggatacgacgtcaaggcgttcacctaccacgacgcacctcgccgt288LeuGlyTyrAspValLysAlaPheThrTyrHisAspAlaProArgArg859095gcgcactccattgctgcacagggtggcgttaactccgcccgcggcaag336AlaHisSerIleAlaAlaGlnGlyGlyValAsnSerAlaArgGlyLys100105110aaggtagacaacgacggcgcataccgccacgtcaaggacaccgtcaag384LysValAspAsnAspGlyAlaTyrArgHisValLysAspThrValLys115120125ggcggcgactaccgtggtcgcgagtccgactgctggcgtctcgccgtc432GlyGlyAspTyrArgGlyArgGluSerAspCysTrpArgLeuAlaVal130135140gagtccgtccgcgtcatcgaccacatgaacgccatcggtgcaccattc480GluSerValArgValIleAspHisMetAsnAlaIleGlyAlaProPhe145150155160gcccgcgaatacggtggcgccttggcaacccgttccttcggtggtgtg528AlaArgGluTyrGlyGlyAlaLeuAlaThrArgSerPheGlyGlyVal165170175caggtctcccgtacctactacacccgtggacaaaccggacagcagctg576GlnValSerArgThrTyrTyrThrArgGlyGlnThrGlyGlnGlnLeu180185190cagctctccaccgcatccgcactacagcgccagatccacctcggctcc624GlnLeuSerThrAlaSerAlaLeuGlnArgGlnIleHisLeuGlySer195200205gtagaaatcttcacccataacgaaatggttgacgtcattgtcaccgaa672ValGluIlePheThrHisAsnGluMetValAspValIleValThrGlu210215220cgtaacggtgaaaagcgctgcgaaggcctgatcatgcgcaacctgatc720ArgAsnGlyGluLysArgCysGluGlyLeuIleMetArgAsnLeuIle225230235240accggcgagctcaccgcacacaccggccatgccgttatcctggcaacc768ThrGlyGluLeuThrAlaHisThrGlyHisAlaValIleLeuAlaThr245250255ggtggctacggcaacgtgtaccacatgtccaccctggccaagaactcc816GlyGlyTyrGlyAsnValTyrHisMetSerThrLeuAlaLysAsnSer260265270aacgcctcggccatcatgcgtgcatacgaagccggcgcatacttcgcg864AsnAlaSerAlaIleMetArgAlaTyrGluAlaGlyAlaTyrPheAla275280285tccccatcgttcatccagttccacccaaccggcctgcctgtgaactcc912SerProSerPheIleGlnPheHisProThrGlyLeuProValAsnSer290295300acctggcagtccaagaccattctgatgtccgagtcgctgcgtaacgac960ThrTrpGlnSerLysThrIleLeuMetSerGluSerLeuArgAsnAsp305310315320ggccgcatctggtcccctaaggaaccgaacgataaccgcgatccaaac1008GlyArgIleTrpSerProLysGluProAsnAspAsnArgAspProAsn325330335accatccctgaggatgagcgcgactacttcctggagcgccgctaccca1056ThrIleProGluAspGluArgAspTyrPheLeuGluArgArgTyrPro340345350gcattcggtaacctcgtcccacgtgacgttgcttcccgtgcgatctcc1104AlaPheGlyAsnLeuValProArgAspValAlaSerArgAlaIleSer355360365cagcagatcaatgctggtctcggtgttggacctctgaacaacgctgca1152GlnGlnIleAsnAlaGlyLeuGlyValGlyProLeuAsnAsnAlaAla370375380tacctggacttccgcgacgccaccgagcgcctcggacaggacaccatc1200TyrLeuAspPheArgAspAlaThrGluArgLeuGlyGlnAspThrIle385390395400cgcgagcgttactccaacctcttcaccatgtacgaagaggcaattggc1248ArgGluArgTyrSerAsnLeuPheThrMetTyrGluGluAlaIleGly405410415gaggacccatactccagcccaatgcgtattgcaccgacctgccacttc1296GluAspProTyrSerSerProMetArgIleAlaProThrCysHisPhe420425430accatgggtggcctctggactgacttcaacgaaatgacgtcactccca1344ThrMetGlyGlyLeuTrpThrAspPheAsnGluMetThrSerLeuPro435440445ggtctgttctgcgcaggcgaagcatcctggacctaccacggtgcaaac1392GlyLeuPheCysAlaGlyGluAlaSerTrpThrTyrHisGlyAlaAsn450455460cgtctgggcgcaaactccctgctctccgcttccgtcgatggctggttc1440ArgLeuGlyAlaAsnSerLeuLeuSerAlaSerValAspGlyTrpPhe465470475480accctgccattcaccatccctaactacctcggcccattgcttggctcc1488ThrLeuProPheThrIleProAsnTyrLeuGlyProLeuLeuGlySer485490495gagcgtctgtcagaggatgcaccagaagcacaggcagcgattgcgcgt1536GluArgLeuSerGluAspAlaProGluAlaGlnAlaAlaIleAlaArg500505510gcacaggctcgcattgaccgcctcatgggcaaccgcccagagtgggtc1584AlaGlnAlaArgIleAspArgLeuMetGlyAsnArgProGluTrpVal515520525ggtgacaacgttcacggacctgagtactaccaccgccagcttggcgat1632GlyAspAsnValHisGlyProGluTyrTyrHisArgGlnLeuGlyAsp530535540atcctgtacttctcctgtggcgtttcccgaaacgtagaagacctccag1680IleLeuTyrPheSerCysGlyValSerArgAsnValGluAspLeuGln545550555560gatggcatcaacaagatccgtgccctccgcgatgacttctggaagaac1728AspGlyIleAsnLysIleArgAlaLeuArgAspAspPheTrpLysAsn565570575atgcgcatcaccggcagcaccgatgagatgaaccaggttctcgaatac1776MetArgIleThrGlySerThrAspGluMetAsnGlnValLeuGluTyr580585590gcagcacgcgtagccgactacatcgacctcggcgaactcatgtgtgtc1824AlaAlaArgValAlaAspTyrIleAspLeuGlyGluLeuMetCysVal595600605gacgccctcgaccgcgacgagtcctgtggcgctcacttccgcgacgac1872AspAlaLeuAspArgAspGluSerCysGlyAlaHisPheArgAspAsp610615620cacctctccgaagatggcgaagcagaacgtgacgacgaaaactggtgc1920HisLeuSerGluAspGlyGluAlaGluArgAspAspGluAsnTrpCys625630635640ttcgtctccgcatgggaaccaggcgagaacggaaccttcgtccgccac1968PheValSerAlaTrpGluProGlyGluAsnGlyThrPheValArgHis645650655gcagaaccactgttcttcgaatccgtcccactgcagacaaggaactac2016AlaGluProLeuPhePheGluSerValProLeuGlnThrArgAsnTyr660665670aag2019Lys<210>116<211>673<212>PRT<213>谷氨酸棒杆菌<400>116MetSerThrHisSerGluThrThrArgProGluPheIleHisProVal151015SerValLeuProGluValSerAlaGlyThrValLeuAspAlaAlaGlu202530ProAlaGlyValProThrLysAspMetTrpGluTyrGlnLysAspHis354045MetAsnLeuValSerProLeuAsnArgArgLysPheArgValLeuVal505560ValGlyThrGlyLeuSerGlyGlyAlaAlaAlaAlaAlaLeuGlyGlu65707580LeuGlyTyrAspValLysAlaPheThrTyrHisAspAlaProArgArg859095AlaHisSerIleAlaAlaGlnGlyGlyValAsnSerAlaArgGlyLys100105110LysValAspAsnAspGlyAlaTyrArgHisValLysAspThrValLys115120125GlyGlyAspTyrArgGlyArgGluSerAspCysTrpArgLeuAlaVal130135140GluSerValArgValIleAspHisMetAsnAlaIleGlyAlaProPhe145150155160AlaArgGluTyrGlyGlyAlaLeuAlaThrArgSerPheGlyGlyVal165170175GlnValSerArgThrTyrTyrThrArgGlyGlnThrGlyGlnGlnLeu180185190GlnLeuSerThrAlaSerAlaLeuGlnArgGlnIleHisLeuGlySer195200205ValGluIlePheThrHisAsnGluMetValAspValIleValThrGlu210215220ArgAsnGlyGluLysArgCysGluGlyLeuIleMetArgAsnLeuIle225230235240ThrGlyGluLeuThrAlaHisThrGlyHisAlaValIleLeuAlaThr245250255GlyGlyTyrGlyAsnValTyrHisMetSerThrLeuAlaLysAsnSer260265270AsnAlaSerAlaIleMetArgAlaTyrGluAlaGlyAlaTyrPheAla275280285SerProSerPheIleGlnPheHisProThrGlyLeuProValAsnSer290295300ThrTrpGlnSerLysThrIleLeuMetSerGluSerLeuArgAsnAsp305310315320GlyArgIleTrpSerProLysGluProAsnAspAsnArgAspProAsn325330335ThrIleProGluAspGluArgAspTyrPheLeuGluArgArgTyrPro340345350AlaPheGlyAsnLeuValProArgAspValAlaSerArgAlaIleSer355360365GlnGlnIleAsnAlaGlyLeuGlyValGlyProLeuAsnAsnAlaAla370375380TyrLeuAspPheArgAspAlaThrGluArgLeuGlyGlnAspThrIle385390395400ArgGluArgTyrSerAsnLeuPheThrMetTyrGluGluAlaIleGly405410415GluAspProTyrSerSerProMetArgIleAlaProThrCysHisPhe420425430ThrMetGlyGlyLeuTrpThrAspPheAsnGluMetThrSerLeuPro435440445GlyLeuPheCysAlaGlyGluAlaSerTrpThrTyrHisGlyAlaAsn450455460ArgLeuGlyAlaAsnSerLeuLeuSerAlaSerValAspGlyTrpPhe465470475480ThrLeuProPheThrIleProAsnTyrLeuGlyProLeuLeuGlySer485490495GluArgLeuSerGluAspAlaProGluAlaGlnAlaAlaIleAlaArg500505510AlaGlnAlaArgIleAspArgLeuMetGlyAsnArgProGluTrpVal515520525GlyAspAsnValHisGlyProGluTyrTyrHisArgGlnLeuGlyAsp530535540IleLeuTyrPheSerCysGlyValSerArgAsnValGluAspLeuGln545550555560AspGlyIleAsnLysIleArgAlaLeuArgAspAspPheTrpLysAsn565570575MetArgIleThrGlySerThrAspGluMetAsnGlnValLeuGluTyr580585590AlaAlaArgValAlaAspTyrIleAspLeuGlyGluLeuMetCysVal595600605AspAlaLeuAspArgAspGluSerCysGlyAlaHisPheArgAspAsp610615620HisLeuSerGluAspGlyGluAlaGluArgAspAspGluAsnTrpCys625630635640PheValSerAlaTrpGluProGlyGluAsnGlyThrPheValArgHis645650655AlaGluProLeuPhePheGluSerValProLeuGlnThrArgAsnTyr660665670Lys<210>117<211>1875<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1875)<223>RXA01312K<400>117atgaacctggtctccccactgaaccgacgcaagttccgtgtcctcgtc48MetAsnLeuValSerProLeuAsnArgArgLysPheArgValLeuVal151015gttggcaccggcctgtccggtggtgctgcagcagcagccctcggcgaa96ValGlyThrGlyLeuSerGlyGlyAlaAlaAlaAlaAlaLeuGlyGlu202530ctcggatacgacgtcaaggcgttcacctaccacgacgcacctcgccgt144LeuGlyTyrAspValLysAlaPheThrTyrHisAspAlaProArgArg354045gcgcactccattgctgcacagggtggcgttaactccgcccgcggcaag192AlaHisSerIleAlaAlaGlnGlyGlyValAsnSerAlaArgGlyLys505560aaggtagacaacgacggcgcataccgccacgtcaaggacaccgtcaag240LysValAspAsnAspGlyAlaTyrArgHisValLysAspThrValLys65707580ggcggcgactaccgtggtcgcgagtccgactgctggcgtctcgccgtc288GlyGlyAspTyrArgGlyArgGluSerAspCysTrpArgLeuAlaVal859095gagtccgtccgcgtcatcgaccacatgaacgccatcggtgcaccattc336GluSerValArgValIleAspHisMetAsnAlaIleGlyAlaProPhe100105110gcccgcgaatacggtggcgccttggcaacccgttccttcggtggtgtg384AlaArgGluTyrGlyGlyAlaLeuAlaThrArgSerPheGlyGlyVal115120125caggtctcccgtacctactacacccgtggacaaaccggacagcagctg432GlnValSerArgThrTyrTyrThrArgGlyGlnThrGlyGlnGlnLeu130135140cagctctccaccgcatccgcactacagcgccagatccacctcggctcc480GlnLeuSerThrAlaSerAlaLeuGlnArgGlnIleHisLeuGlySer145150155160gtagaaatcttcacccataacgaaatggttgacgtcattgtcaccgaa528ValGluIlePheThrHisAsnGluMetValAspVa1IleValThrGlu165170175cgtaacggtgaaaagcgctgcgaaggcctgatcatgcgcaacctgatc576ArgAsnGlyGluLysArgCysGluGlyLeuIleMetArgAsnLeuIle180185190accggcgagctcaccgcacacaccggccatgccgttatcctggcaacc624ThrGlyGluLeuThrAlaHisThrGlyHisAlaValIleLeuAlaThr195200205ggtggctacggcaacgtgtaccacatgtccaccctggccaagaactcc672GlyGlyTyrGlyAsnValTyrHisMetSerThrLeuAlaLysAsnSer210215220aacgcctcggccatcatgcgtgcatacgaagccggcgcatacttcgcg720AsnAlaSerAlaIleMetArgAlaTyrGluAlaGlyAlaTyrPheAla225230235240tccccatcgttcatccagttccacccaaccggcctgcctgtgaactcc768SerProSerPheIleGlnPheHisProThrGlyLeuProValAsnSer245250255acctggcagtccaagaccattctgatgtccgagtcgctgcgtaacgac816ThrTrpGlnSerLysThrIleLeuMetSerGluSerLeuArgAsnAsp260265270ggccgcatctggtcccctaaggaaccgaacgataaccgcgatccaaac864GlyArgIleTrpSerProLysGluProAsnAspAsnArgAspProAsn275280285accatccctgaggatgagcgcgactacttcctggagcgccgctaccca912ThrIleProGluAspGluArgAspTyrPheLeuGluArgArgTyrPro290295300gcattcggtaacctcgtcccacgtgacgttgcttcccgtgcgatctcc960AlaPheGlyAsnLeuValProArgAspValAlaSerArgAlaIleSer305310315320cagcagatcaatgctggtctcggtgttggacctctgaacaacgctgca1008GlnGlnIleAsnAlaGlyLeuGlyValGlyProLeuAsnAsnAlaAla325330335tacctggacttccgcgacgccaccgagcgcctcggacaggacaccatc1056TyrLeuAspPheArgAspAlaThrGluArgLeuGlyGlnAspThrIle340345350cgcgagcgttactccaacctcttcaccatgtacgaagaggcaattggc1104ArgGluArgTyrSerAsnLeuPheThrMetTyrGluGluAlaIleGly355360365gaggacccatactccagcccaatgcgtattgcaccgacctgccacttc1152GluAspProTyrSerSerProMetArgIleAlaProThrCysHisPhe370375380accatgggtggcctctggactgacttcaacgaaatgacgtcactccca1200ThrMetGlyGlyLeuTrpThrAspPheAsnGluMetThrSerLeuPro385390395400ggtctgttctgcgcaggcgaagcatcctggacctaccacggtgcaaac1248GlyLeuPheCysAlaGlyGluAlaSerTrpThrTyrHisGlyAlaAsn405410415cgtctgggcgcaaactccctgctctccgcttccgtcgatggctggttc1296ArgLeuGlyAlaAsnSerLeuLeuSerAlaSerValAspGlyTrpPhe420425430accctgccattcaccatccctaactacctcggcccattgcttggctcc1344ThrLeuProPheThrIleProAsnTyrLeuGlyProLeuLeuGlySer435440445gagcgtctgtcagaggatgcaccagaagcacaggcagcgattgcgcgt1392GluArgLeuSerGluAspAlaProGluAlaGlnAlaAlaIleAlaArg450455460gcacaggctcgcattgaccgcctcatgggcaaccgcccagagtgggtc1440AlaGlnAlaArgIleAspArgLeuMetGlyAsnArgProGluTrpVal465470475480ggtgacaacgttcacggacctgagtactaccaccgccagcttggcgat1488GlyAspAsnValHisGlyProGluTyrTyrHisArgGlnLeuGlyAsp485490495atcctgtacttctcctgtggcgtttcccgaaacgtagaagacctccag1536IleLeuTyrPheSerCysGlyValSerArgAsnValGluAspLeuGln500505510gatggcatcaacaagatccgtgccctccgcgatgacttctggaagaac1584AspGlyIleAsnLysIleArgAlaLeuArgAspAspPheTrpLysAsn515520525atgcgcatcaccggcagcaccgatgagatgaaccaggttctcgaatac1632MetArgIleThrGlySerThrAspGluMetAsnGlnValLeuGluTyr530535540gcagcacgcgtagccgactacatcgacctcggcgaactcatgtgtgtc1680AlaAlaArgValAlaAspTyrIleAspLeuGlyGluLeuMetCysVal545550555560gacgccctcgaccgcgacgagtcctgtggcgctcacttccgcgacgac1728AspAlaLeuAspArgAspGluSerCysGlyAlaHisPheArgAspAsp565570575cacctctccgaagatggcgaagcagaacgtgacgacgaaaactggtgc1776HisLeuSerGluAspGlyGluAlaGluArgAspAspGluAsnTrpCys580585590ttcgtctccgcatgggaaccaggcgagaacggaaccttcgtccgccac1824PheValSerAlaTrpGluProGlyGluAsnGlyThrPheValArgHis595600605gcagaaccactgttcttcgaatccgtcccactgcagacaaggaactac1872AlaGluProLeuPhePheGluSerValProLeualnThrArgAsnTyr610615620aag1875Lys625<210>118<211>625<212>PRT<213>谷氨酸棒杆菌<400>118MetAsnLeuValSerProLeuAsnArgArgLysPheArgValLeuVal151015ValGlyThrGlyLeuSerGlyGlyAlaAlaAlaAlaAlaLeuGlyGlu202530LeuGlyTyrAspValLysAlaPheThrTyrHisAspAlaProArgArg354045AlaHisSerIleAlaAlaGlnGlyGlyValAsnSerAlaArgGlyLys505560LysValAspAsnAspGlyAlaTyrArgHisValLysAspThrValLys65707580GlyGlyAspTyrArgGlyArgGluSerAspCysTrpArgLeuAlaVal859095GluSerValArgValIleAspHisMetAsnAlaIleGlyAlaProPhe100105110AlaArgGluTyrGlyGlyAlaLeuAlaThrArgSerPheGlyGlyVal115120125GlnValSerArgThrTyrTyrThrArgGlyGlnThrGlyGlnGlnLeu130135140GlnLeuSerThrAlaSerAlaLeuGlnArgGlnIleHisLeuGlySer145150155160ValGluIlePheThrHisAsnGluMetValAspValIleValThrGlu165170175ArgAsnGlyGluLysArgCysGluGlyLeuIleMetArgAsnLeuIle180185190ThrGlyGluLeuThrAlaHisThrGlyHisAlaValIleLeuAlaThr195200205GlyGlyTyrGlyAsnValTyrHisMetSerThrLeuAlaLysAsnSer210215220AsnAlaSerAlaIleMetArgAlaTyrGluAlaGlyAlaTyrPheAla225230235240SerProSerPheIleGlnPheHisProThrGlyLeuProValAsnSer245250255ThrTrpGlnSerLysThrIleLeuMetSerGluSerLeuArgAsnAsp260265270GlyArgIleTrpSerProLysGluProAsnAspAsnArgAspProAsn275280285ThrIleProGluAspGluArgAspTyrPheLeuGluArgArgTyrPro290295300AlaPheGlyAsnLeuValProArgAspValAlaSerArgAlaIleSer305310315320GlnGlnIleAsnAlaGlyLeuGlyValGlyProLeuAsnAsnAlaAla325330335TyrLeuAspPheArgAspAlaThrGluArgLeuGlyGlnAspThrIle340345350ArgGluArgTyrSerAsnLeuPheThrMetTyrGluGluAlaIleGly355360365GluAspProTyrSerSerProMetArgIleAlaProThrCysHisPhe370375380ThrMetGlyGlyLeuTrpThrAspPheAsnGluMetThrSerLeuPro385390395400GlyLeuPheCysAlaGlyGluAlaSerTrpThrTyrHisGlyAlaAsn405410415ArgLeuGlyAlaAsnSerLeuLeuSerAlaSerValAspGlyTrpPhe420425430ThrLeuProPheThrIleProAsnTyrLeuGlyProLeuLeuGlySer435440445GluArgLeuSerGluAspAlaProGluAlaGlnAlaAlaIleAlaArg450455460AlaGlnAlaArgIleAspArgLeuMetGlyAsnArgProGluTrpVal465470475480GlyAspAsnValHisGlyProGluTyrTyrHisArgGlnLeuGlyAsp485490495IleLeuTyrPheSerCysGlyValSerArgAsnValGluAspLeuGln500505510AspGlyIleAsnLysIleArgAlaLeuArgAspAspPheTrpLysAsn515520525MetArgIleThrGlySerThrAspGluMetAsaGlnValLeuGluTyr530535540AlaAlaArgValAlaAspTyrIleAspLeuGlyGluLeuMetCysVal545550555560AspAlaLeuAspArgAspGluSerCysGlyAlaHisPheArgAspAsp565570575HisLeuSerGluAspGlyGluAlaGluArgAspAspGluAsnTrpCys580585590PheValSerAlaTrpGluProGlyGluAsnGlyThrPheValArgHis595600605AlaGluProLeuPhePheGluSerValProLeuGlnThrArgAsaTyr610615620Lys625<210>119<211>1107<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1107)<223>RXA01350E<400>119ttggggcaattcgggggtaattttgcagtatcgtcaagatcacccaaa48MetGlyGlnPheGlyGlyAsnPheAlaValSerSerArgSerProLys151015actggtggctgttctcttttaagcgggatagcatgggttattagagga96ThrGlyGlyCysSerLeuLeuSerGlyIleAlaTrpValLeuArgGly202530ccccctacaaggattgaggattgtttaatgaattccccgcagaacgtc144ProProThrArgIleGluAspCysLeuMetAsnSerProGlnAsnVal354045tccaccaagaaggtcaccgtcaccggcgcagctggtcaaatctcttat192SerThrLysLysValThrValThrGlyAlaAlaGlyGlnIleSerTyr505560tcactgttgtggcgcatcgccaacggtgaagtattcggcaccgacacc240SerLeuLeuTrpArgIleAlaAsnGlyGluValPheGlyThrAspThr65707580cctgtagaactgaaacttctggagatccctcaggctcttggcggggca288ProValGluLeuLysLeuLeuGluIleProGlnAlaLeuGlyGlyAla859095gagggtgtggctatggaacttctggattctgccttccccctcctgcga336GluGlyValAlaMetGluLeuLeuAspSerAlaPheProLeuLeuArg100105110aacatcaccatcaccgcggatgccaatgaggcattcgacggcgctaat384AsnIleThrIleThrAlaAspAlaAsnGluAlaPheAspGlyAlaAsn115120125gcggcgtttttggtcggtgcgaagcctcgcggaaaaggcgaagagcgc432AlaAlaPheLeuValGlyAlaLysProArgGlyLysGlyGluGluArg130135140gcagatttgctggctaacaacggcaagattttcggacctcaaggtaaa480AlaAspLeuLeuAlaAsnAsnGlyLysIlePheGlyProGlnGlyLys145150155160gctatcaatgacaacgccgcagatgacattcgtgtcctagttgttgga528AlaIleAsnAspAsnAlaAlaAspAspIleArgValLeuValValGly165170175aacccagcgaacaccaacgcgttgattgcttcagctgcggccccagat576AsnProAlaAsnThrAsnAlaLeuIleAlaSerAlaAlaAlaProAsp180185190gttccagcatcccgcttcaacgcaatgatgcgccttgatcacaaccgt624ValProAlaSerArgPheAsnAlaMetMetArgLeuAspHisAsnArg195200205gcgatctcccagctggccaccaagcttggccgtggatctgcggaattt672AlaIleSerGlnLeuAlaThrLysLeuGlyArgGlySerAlaGluPhe210215220aacaacattgtggtctggggaaatcactccgcaacccagttcccagac720AsnAsnIleValValTrpGlyAsnHisSerAlaThrGlnPheProAsp225230235240atcacctacgcaaccgttggtggagaaaaggtcactgacctggttgat768IleThrTyrAlaThrValGlyGlyGluLysValThrAspLeuValAsp245250255cacgattggtatgtggaggagttcattcctcgcgtggctaaccgtggc816HisAspTrpTyrValGluGluPheIleProArgValAlaAsnArgGly260265270gctgaaatcattgaggtccgtggaaagtcttctgcagcttctgcagca864AlaGluIleIleGluValArgGlyLysSerSerAlaAlaSerAlaAla275280285tcctctgcgattgatcacatgcgcgattgggtacagggcaccgaggcg912SerSerAlaIleAspHisMetArgAspTrpValGlnGlyThrGluAla290295300tggtcctctgcggcaattccttccaccggtgcatacggcattcctgag960TrpSerSerAlaAlaIleProSerThrGlyAlaTyrGlyIleProGlu305310315320ggcatttttgtcggtctgccaaccgtatcccgcaacggtgagtgggaa1008GlyIlePheValGlyLeuProThrValSerArgAsnGlyGluTrpGlu325330335atcgttgaaggcctggagatttccgatttccagcgcgcccgcatcgac1056IleValGluGlyLeuGluIleSerAspPheGlnArgAlaArgIleAsp340345350gcgaatgctcaggaattgcaggccgagcgcgaggcagtgcgcgacttg1104AlaAsnAlaGlnGluLeuGlnAlaGluArgGluAlaValArgAspLeu355360365ctc1107Leu<210>120<211>369<212>PRT<213>谷氨酸棒杆菌<400>120MetGlyGlnPheGlyGlyAsnPheAlaValSerSerArgSerProLys151015ThrGlyGlyCysSerLeuLeuSerGlyIleAlaTrpValLeuArgGly202530ProProThrArgIleGluAspCysLeuMetAsnSerProGlnAsnVal354045SerThrLysLysValThrValThrGlyAlaAlaGlyGlnIleSerTyr505560SerLeuLeuTrpArgIleAlaAsnGlyGluValPheGlyThrAspThr65707580ProValGluLeuLysLeuLeuGluIleProGlnAlaLeuGlyGlyAla859095GluGlyValAlaMetGluLeuLeuAspSerAlaPheProLeuLeuArg100105110AsnIleThrIleThrAlaAspAlaAsnGluAlaPheAspGlyAlaAsn115120125AlaAlaPheLeuValGlyAlaLysProArgGlyLysGlyGluGluArs130135140AlaAspLeuLeuAlaAsnAsnGlyLysIlePheGlyProGlnGlyLys145150155160AlaIleAsnAspAsnAlaAlaAspAspIleArgValLeuValValGly165170175AsnProAlaAsnThrAsnAlaLeuIleAlaSerAlaAlaAlaProAsp180185190ValProAlaSerArgPheAsnAlaMetMetArgLeuAspHisAsnArg195200205AlaIleSerGlnLeuAlaThrLysLeuGlyArgGlySerAlaGluPhe210215220AsnAsnIleValValTrpGlyAsnHisSerAlaThrGlnPheProAsp225230235240IleThrTyrAlaThrValGlyGlyGluLysValThrAspLeuValAsp245250255HisAspTrpTyrValGluGluPheIleProArgValAlaAsaArgGly260265270AlaGluIleIleGluValArgGlyLysSerSerAlaAlaSerAlaAla275280285SerSerAlaIleAspHisMetArgAspTrpValGlnGlyThrGluAla290295300TrpSerSerAlaAlaIleProSerThrGlyAlaTyrGlyIleProGlu305310315320GlyIlePheValGlyLeuProThrValSerArgAsnGlyGluTrpGlu325330335lleValGluGlyLeuGluIleSerAspPheGlnArgAlaArgIleAsp340345350AlaAsnAlaGlnGluLeuGlnAlaGluArgGluAlaValArgAspLeu355360365Leu<210>121<211>984<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(984)<223>RXA01350W<400>121atgaattccccgcagaacgtctccaccaagaaggtcaccgtcaccggc48MetAsnSerProGlnAsnValSerThrLysLysValThrValThrGly151015gcagctggtcaaatctcttattcactgttgtggcgcatcgccaacggt96AlaAlaGlyGlnIleSerTyrSerLeuLeuTrpArgIleAlaAsnGly202530gaagtattcggcaccgacacccctgtagaactgaaacttctggagatc144GluValPheGlyThrAspThrProValGluLeuLysLeuLeuGluIle354045cctcaggctcttggcggggcagagggtgtggctatggaacttctggat192ProGlnAlaLeuGlyGlyAlaGluGlyValAlaMetGluLeuLeuAsp505560tctgccttccccctcctgcgaaacatcaccatcaccgcggatgccaat240SerAlaPheProLeuLeuArgAsnIleThrIleThrAlaAspAlaAsn65707580gaggcattcgacggcgctaatgcggcgtttttggtcggtgcgaagcct288GluAlaPheAspGlyAlaAsnAlaAlaPheLeuValGlyAlaLysPro859095cgcggaaaaggcgaagagcgcgcagatttgctggctaacaacggcaag336ArgGlyLysGlyGluGluArgAlaAspLeuLeuAlaAsnAsnGlyLys100105110attttcggacctcaaggtaaagctatcaatgacaacgccgcagatgac384IlePheGlyProGlnGlyLysAlaIleAsnAspAsnAlaAlaAspAsp115120125attcgtgtcctagttgttggaaacccagcgaacaccaacgcgttgatt432IleArgValLeuValValGlyAsnProAlaAsnThrAsnAlaLeuIle130135140gcttcagctgcggccccagatgttccagcatcccgcttcaacgcaatg480AlaSerAlaAlaAlaProAspValProAlaSerArgPheAsnAlaMet145150155160atgcgccttgatcacaaccgtgcgatctcccagctggccaccaagctt528MetArgLeuAspHisAsnArgAlaIleSerGlnLeuAlaThrLysLeu165170175ggccgtggatctgcggaatttaacaacattgtggtctggggaaatcac576GlyArgGlySerAlaGluPheAsnAsnIleValValTrpGlyAsnHis180185190tccgcaacccagttcccagacatcacctacgcaaccgttggtggagaa624SerAlaThrGlnPheProAspIleThrTyrAlaThrValGlyGlyGlu195200205aaggtcactgacctggttgatcacgattggtatgtggaggagttcatt672LysValThrAspLeuValAspHisAspTrpTyrValGluGluPheIle210215220cctcgcgtggctaaccgtggcgctgaaatcattgaggtccgtggaaag720ProArgValAlaAsnArgGlyAlaGluIleIleGluValArgGlyLys225230235240tcttctgcagcttctgcagcatcctctgcgattgatcacatgcgcgat768SerSerAlaAlaSerAlaAlaSerSerAlaIleAspHisMetArgAsp245250255tgggtacagggcaccgaggcgtggtcctctgcggcaattccttccacc816TrpValGlnGlyThrGluAlaTrpSerSerAlaAlaIleProSerThr260265270ggtgcatacggcattcctgagggcatttttgtcggtctgccaaccgta864GlyAlaTyrGlyIleProGluGlyIlePheValGlyLeuProThrVal275280285tcccgcaacggtgagtgggaaatcgttgaaggcctggagatttccgat912SerArgAsnGlyGluTrpGluIleValGluGlyLeuGluIleSerAsp290295300ttccagcgcgcccgcatcgacgcgaatgctcaggaattgcaggccgag960PheGlnArgAlaArgIleAspAlaAsnAlaGlnGluLeuGlnAlaGlu305310315320cgcgaggcagtgcgcgacttgctc984ArgGluAlaValArgAspLeuLeu325<210>122<211>328<212>PRT<213>谷氨酸棒杆菌<400>122MetAsnSerProGlnAsnValSerThrLysLysValThrValThrGly151015AlaAlaGlyGlnIleSerTyrSerLeuLeuTrpArgIleAlaAsnGly202530GluValPheGlyThrAspThrProValGluLeuLysLeuLeuGluIle354045ProGlnAlaLeuGlyGlyAlaGluGlyValAlaMetGluLeuLeuAsp505560SerAlaPheProLeuLeuArgAsnIleThrIleThrAlaAspAlaAsn65707580GluAlaPheAspGlyAlaAsnAlaAlaPheLeuValGlyAlaLysPro859095ArgGlyLysGlyGluGluArgAlaAspLeuLeuAlaAsnAsnGlyLys100105110IlePheGlyProGlnGlyLysAlaIleAsnAspAsnAlaAlaAspAsp115120125IleArgValLeuValValGlyAsnProAlaAsnThrAsnAlaLeuIle130135140AlaSerAlaAlaAlaProAspValProAlaSerArgPheAsnAlaMet145150155160MetArgLeuAspHisAsnArgAlaIleSerGlnLeuAlaThrLysLeu165170175GlyArgGlySerAlaGluPheAsnAsnIleValValTrpGlyAsnHis180185190SerAlaThrGlnPheProAspIleThrTyrAlaThrValGlyGlyGlu195200205LysValThrAspLeuValAspHisAspTrpTyrValGluGluPheIle210215220ProArgValAlaAsnArgGlyAlaGluIleIleGluValArgGlyLys225230235240SerSerAlaAlaSerAlaAlaSerSerAlaIleAspHisMetArgAsp245250255TrpValGlnGlyThrGluAlaTrpSerSerAlaAlaIleProSerThr260265270GlyAlaTyrGlyIleProGluGlyIlePheValGlyLeuProThrVal275280285SerArgAsnGlyGluTrpGluIleValGluGlyLeuGluIleSerAsp290295300PheGlnArgAlaArgIleAspAlaAsnAlaGlnGluLeuGlnAlaalu305310315320ArgGluAlaValArgAspLeuLeu325<210>123<211>870<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(870)<223>RXA01393W<400>123atgaaccccattcaactggacactttgctctcaatcattgatgaaggc48MetAsnProIleGlnLeuAspThrLeuLeuSerIleIleAspGluGly151015agcttcgaaggcgcctccttagccctttccatttccccctcggcggtg96SerPheGluGlyAlaSerLeuAlaLeuSerIleSerProSerAlaVal202530agtcagcgcgttaaagctctcgagcatcacgtgggtcgagtgttggta144SerGlnArgValLysAlaLeuGluHisHisValGlyArgValLeuVal354045tcgcgcacccaaccggccaaagcaaccgaagcgggtgaagtccttgtg192SerArgThrGlnProAlaLysAlaThrGluAlaGlyGluValLeuVal505560caagcagcgcggaaaatggtgttgctgcaagcagaaactaaagcgcaa240GlnAlaAlaArgLysMetValLeuLeuGlnAlaGluThrLysAlaGln65707580ctatctggacgccttgctgaaatcccgttaaccatcgccatcaacgca288LeuSerGlyArgLeuAlaGluIleProLeuThrIleAlaIleAsnAla859095gattcgctatccacatggtttcctcccgtgttcaacgaggtagcttct336AspSerLeuSerThrTrpPheProProValPheAsnGluValAlaSer100105110tggggtggagcaacgctcacgctgcgcttggaagatgaagcgcacaca384TrpGlyGlyAlaThrLeuThrLeuArgLeuGluAspGluAlaHisThr115120125ttatccttgctgcggcgtggagatgttttaggagcggtaacccgtgaa432LeuSerLeuLeuArgArgGlyAspValLeuGlyAlaValThrArgGlu130135140gctaatcccgtggcgggatgtgaagtagtagaacttggaaccatgcgc480AlaAsnProValAlaGlyCysGluValValGluLeuGlyThrMetArg145150155160cacttggccattgcaaccccctcattgcgggatgcctacatggttgat528HisLeuAlaIleAlaThrProSerLeuArgAspAlaTyrMetValAsp165170175gggaaactagattgggctgcgatgcccgtcttacgcttcggtcccaaa576GlyLysLeuAspTrpAlaAlaMetProValLeuArgPheGlyProLys180185190gatgtgcttcaagaccgtgacctggacgggcgcgtcgatggtcctgtg624AspValLeuGlnAspArgAspLeuAspGlyArgValAspGlyProVal195200205gggcgcaggcgcgtatccattgtcccgtcggcggaaggttttggtgag672GlyArgArgArgValSerIleValProSerAlaGluGlyPheGlyGlu210215220gcaattcgccgaggccttggttggggacttcttcccgaaacccaagct720AlaIleArgArgGlyLeuGlyTrpGlyLeuLeuProGluThrGlnAla225230235240gctcccatgctaaaagcaggagaagtgatcctcctcgatgagataccc768AlaProMetLeuLysAlaGlyGluValIleLeuLeuAspGluIlePro245250255attgacacaccgatgtattggcaacgatggcgcctggaatctagatct816IleAspThrProMetTyrTrpGlnArgTrpArgLeuGluSerArgSer260265270ctagctagactcacagacgccgtcgttgatgcagcaatcgagggattg864LeuAlaArgLeuThrAspAlaValValAspAlaAlaIleGluGlyLeu275280285cggcct870ArgPro290<210>124<211>290<212>PRT<213>谷氨酸棒杆菌<400>124MetAsnProIleGlnLeuAspThrLeuLeuSerIleIleAspGluGly151015SerPheGluGlyAlaSerLeuAlaLeuSerIleSerProSerAlaVal202530SerGlnArgValLysAlaLeuGluHisHisValGlyArgValLeuVal354045SerArgThrGlnProAlaLysAlaThrGluAlaGlyGluValLeuVal505560GlnAlaAlaArgLysMetValLeuLeuGlnAlaGluThrLysAlaGln65707580LeuSerGlyArgLeuAlaGluIleProLeuThrIleAlaIleAsnAla859095AspSerLeuSerThrTrpPheProProValPheAsnGluValAlaSer100105110TrpGlyGlyAlaThrLeuThrLeuArgLeuGluAspGluAlaHisThr115120125LeuSerLeuLeuArgArgGlyAspValLeuGlyAlaValThrArgGlu130135140AlaAsnProValAlaGlyCysGluValValGluLeuGlyThrMetArg145150155160HisLeuAlaIleAlaThrProSerLeuArgAspAlaTyrMetValAsp165170175GlyLysLeuAspTrpAlaAlaMetProValLeuArgPheGlyProLys180185190AspValLeuGlnAspArgAspLeuAspGlyArgValAspGlyProVal195200205GlyArgArgArgValSerIleValProSerAlaGluGlyPheGlyGlu210215220AlaIleArgArgGlyLeuGlyTrpGlyLeuLeuProGluThrGlnAla225230235240AlaProMetLeuLysAlaGlyGluValIleLeuLeuAspGluIlePro245250255IleAspThrProMetTyrTrpGlnArgTrpArgLeuGluSerArgSer260265270LeuAlaArgLeuThrAspAlaValValAspAlaAlaIleGluGlyLeu275280285ArgPro290<210>125<211>948<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(948)<223>RXA02021W<400>125atgagtgaaaacattcgcggagcccaagcagttggaatcgcaaatatc48MetSerGluAsnIleArgGlyAlaGlnAlaValGlyIleAlaAsnIle151015gccatggacgggaccatcctggacacgtggtacccagaaccccaaatt96AlaMetAspGlyThrIleLeuAspThrTrpTyrProGluProGlnIle202530ttcaacccggatcagtgggctgaacgctacccattggaagtgggcacc144PheAsnProAspGlnTrpAlaGluArgTyrProLeuGluValGlyThr354045acacgcctcggagcaaacgaactcaccccacggatgctgcagttggta192ThrArgLeuGlyAlaAsnGluLeuThrProArgMetLeuGlnLeuVal505560aaactggaccaagatcgcctcgtcgaacaggtagcagtccgcaccgtt240LysLeuAspGlnAspArgLeuValGluGlnValAlaValArgThrVal65707580atccccgatctgtctcaacctccagtagacgcgcacgatgtttacctg288IleProAspLeuSerGlnProProValAspAlaHisAspValTyrLeu859095cgcctccacctgctttcccaccggctggtccgcccccacgaaatgcac336ArgLeuHisLeuLeuSerHisArgLeuValArgProHisGluMetHis100105110atgcaaaacaccttggagctgctgtccgacgtggtgtggacaaacaag384MetGlnAsnThrLeuGluLeuLeuSerAspValValTrpThrAsnLys115120125ggcccttgccttcctgaaaactttgagtgggtgcgtggtgctctgcgg432GlyProCysLeuProGluAsnPheGluTrpValArgGlyAlaLeuArg130135140tcccgcggactcatccacgtctactgtgtggaccgtcttccccgcatg480SerArgGlyLeuIleHisValTyrCysValAspArgLeuProArgMet145150155160gtcgactatgtggttccccctggagtccgcatctccgaagcagaacgc528ValAspTyrValValProProGlyValArgIleSerGluAlaGluArg165170175gtgcgcctaggtgcataccttgctccgggtacctctgtgctgcgtgaa576ValArgLeuGlyAlaTyrLeuAlaProGlyThrSerValLeuArgGlu180185190ggtttcgtgtctttcaactccggcaccttgggtgccgcaaaggtggaa624GlyPheValSerPheAsnSerGlyThrLeuGlyAlaAlaLysValGlu195200205ggccgcctgagttccggtgtggtcatcggtgaaggttccgagattgga672GlyArgLeuSerSerGlyValValIleGlyGluGlySerGluIleGly210215220ctgtcttctactattcagtccccgagagatgaacagcgccgccgtttg720LeuSerSerThrIleGlnSerProArgAspGluGlnArgArgArgLeu225230235240ccgttgagcatcggccaaaactgcaactttggtgtcagctccggaatc768ProLeuSerIleGlyGlnAsnCysAsnPheGlyValSerSerGlyIle245250255atcggagtcagtctgggagacaattgcgacatcggaaataacattgtc816IleGlyValSerLeuGlyAspAsnCysAspIleGlyAsnAsnIleVal260265270ttggatggagatacccccatttggttcgcagccgatgaggagttacgc864LeuAspGlyAspThrProIleTrpPheAlaAlaAspGluGluLeuArg275280285actatcgactccatcgaaggccaagcaaattggtcaatcaagcgtgaa912ThrIleAspSerIleGluGlyGlnAlaAsnTrpSerIleLysArgGlu290295300tccggcttccatgagccagttgcccgcctcaaagct948SerGlyPheHisGluProValAlaArgLeuLysAla305310315<210>126<211>316<212>PRT<213>谷氨酸棒杆菌<400>126MetSerGluAsnIleArgGlyAlaGlnAlaValGlyIleAlaAsnIle151015AlaMetAspGlyThrIleLeuAspThrTrpTyrProGluProGlnIle202530PheAsnProAspGlnTrpAlaGluArgTyrProLeuGluValGlyThr354045ThrArgLeuGlyAlaAsnGluLeuThrProArgMetLeuGlnLeuVal505560LysLeuAspGlnAspArgLeuValGluGlnValAlaValArgThrVal65707580IleProAspLeuSerGlnProProValAspAlaHisAspValTyrLeu859095ArgLeuHisLeuLeuSerHisArgLeuValArgProHisGluMetHis100105110MetGlnAsnThrLeuGluLeuLeuSerAspValValTrpThrAsnLys115120125GlyProCysLeuProGluAsnPheGluTrpValArgGlyAlaLeuArg130135140SerArgGlyLeuIleHisValTyrCysValAspArgLeuProArgMet145150155160ValAspTyrValValProProGlyValArgIleSerGluAlaGluArg165170175ValArgLeuGlyAlaTyrLeuAlaProGlyThrSerValLeuArgGlu180185190GlyPheValSerPheAsnSerGlyThrLeuGlyAlaAlaLysValGlu195200205GlyArgLeuSerSerGlyValValIleGlyGluGlySerGluIleGly210215220LeuSerSerThrIleGlnSerProArgAspGluGlnArgArgArgLeu225230235240ProLeuSerIleGlyGlnAsnCysAsnPheGlyValSerSerGlyIle245250255IleGlyValSerLeuGlyAspAsnCysAspIleGlyAsnAsnIleVal260265270LeuAspGlyAspThrProIleTrpPheAlaAlaAspGluGluLeuArg275280285ThrIleAspSerIleGluGlyGlnAlaAsnTrpSerIleLysArgGlu290295300SerGlyPheHisGluProValAlaArgLeuLysAla305310315<210>127<211>1188<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1188)<223>RXA02022E<400>127ttgggctccgcgaatgttttcactcattttttaatcgaccgcttccat48MetGlySerAlaAsnValPheThrHisPheLeuIleAspArgPheHis151015catgttttaactaaggtttgtaggcttaaacctgtgaactctgaactc96HisValLeuThrLysValCysArgLeuLysProValAsnSerGluLeu202530aaaccaggattagatctcctcggcgacccaattgtccttactcaacgt144LysProGlyLeuAspLeuLeuGlyAspProIleValLeuThrGlnArg354045ttggtagatataccgagtccgtcgggtcaggaaaagcagattgctgat192LeuValAspIleProSerProSerGlyGlnGluLysGlnIleAlaAsp505560gaaattgaagatgcccttcggaaccttaatctacctggtgtagaggtc240GluIleGluAspAlaLeuArgAsnLeuAsnLeuProGlyValGluVal65707580ttccgcttcaacaacaacgttcttgctcgcacgaacaggggattggcc288PheArgPheAsnAsnAsnValLeuAlaArgThrAsnArgGlyLeuAla859095tcgagggtcatgcttgctggtcatatcgatacagtgccgatcgcggac336SerArgValMetLeuAlaGlyHisIleAspThrValProIleAlaAsp100105110aatctgccaagccgtgtggaagacggcatcatgtatggctgtggcacc384AsnLeuProSerArgValGluAspGlyIleMetTyrGlyCysGlyThr115120125gtcgatatgaaatctgggttggcggtgtatttgcatacttttgccacc432ValAspMetLysSerGlyLeuAlaValTyrLeuHisThrPheAlaThr130135140ttggccacgtcgactgagcttaaacatgatctgacgctgattgcgtat480LeuAlaThrSerThrGluLeuLysHisAspLeuThrLeuIleAlaTyr145150155160gagtgcgaggaagttgctgatcacctcaatggtttgggccacattcgc528GluCysGluGluValAlaAspHisLeuAsnGlyLeuGlyHisIleArg165170175gatgagcatccggagtggttggcggctgatttggcgttgttgggtgag576AspGluHisProGluTrpLeuAlaAlaAspLeuAlaLeuLeuGlyGlu180185190cctactggcggctggattgaggcgggctgccagggcaatctgcgcatc624ProThrGlyGlyTrpIleGluAlaGlyCysGlnGlyAsnLeuArgIle195200205aaggtgacggcgcatggtgtgcgtgcccattcggcgagaagctggttg672LysValThrAlaHisGlyValArgAlaHisSerAlaArgSerTrpLeu210215220ggtgataatgcgatgcataagttgtcgccgatcatttcgaaggttgct720GlyAspAsnAlaMetHisLysLeuSerProIleIleSerLysValAla225230235240gcgtataaggccgcagaagtcaacattgatggcttgacctaccgtgaa768AlaTyrLysAlaAlaGluValAsnIleAspGlyLeuThrTyrArgGlu245250255ggcctcaacatcgttttctgcgaatcgggcgtggcaaacaacgtcatt816GlyLeuAsnIleValPheCysGluSerGlyValAlaAsnAsnValIle260265270ccagacctcgcgtggatgaacctcaacttccgtttcgcgccgaatcgc864ProAspLeuAlaTrpMetAsnLeuAsnPheArgPheAlaProAsnArg275280285gatctcaacgaggcgatcgagcatgtcgtcgaaacgcttgagcttgac912AspLeuAsnGluAlaIleGluHisValValGluThrLeuGluLeuAsp290295300ggtcaagacggcatcgaatgggccgtagaagacggggcaggcggtgcc960GlyGlnAspGlyIleGluTrpAlaValGluAspGlyAlaGlyGlyAla305310315320cttccaggcttggggcagcaggtgacaagcgggcttatcgacgccgtc1008LeuProGlyLeuGlyGlnGlnValThrSerGlyLeuIleAspAlaVal325330335ggccgcgaaaaaatccgcgcaaaattcggctggaccgatgtctcacgt1056GlyArgGluLysIleArgAlaLysPheGlyTrpThrAspValSerArg340345350ttttcagccatgggaattccagccctaaactttggcgctggtgatcca1104PheSerAlaMetGlyIleProAlaLeuAsnPheGlyAlaGlyAspPro355360365agtttcgcgcataaacgcgacgagcagtgcccagtggagcaaatcacg1152SerPheAlaHisLysArgAspGluGlnCysProValaluGlnIleThr370375380gatgtggcagcaattttgaagcagtacctgagcgag1188AspValAlaAlaIleLeuLysGlnTyrLeuSerGlu385390395<210>128<211>396<212>PRT<213>谷氨酸棒杆菌<400>128MetGlySerAlaAsnValPheThrHisPheLeuIleAspArgPheHis151015HisValLeuThrLysValCysArgLeuLysProValAsnSerGluLeu202530LysProGlyLeuAspLeuLeuGlyAspProIleValLeuThrGlnArg354045LeuValAspIleProSerProSerGlyGlnGluLysGlnIleAlaAsp505560GluIleGluAspAlaLeuArgAsnLeuAsnLeuProGlyValGluVal65707580PheArgPheAsnAsnAsnValLeuAlaArgThrAsnArgGlyLeuAla859095SerArgValMetLeuAlaGlyHisIleAspThrValProIleAlaAsp100105110AsnLeuProSerArgValGluAspGlyIleMetTyrGlyCysGlyThr115120125ValAspMetLysSerGlyLeuAlaValTyrLeuHisThrPheAlaThr130135140LeuAlaThrSerThrGluLeuLysHisAspLeuThrLeuIleAlaTyr145150155160GluCysGluGluValAlaAspHisLeuAsnGlyLeuGlyHisIleArg165170175AspGluHisProGluTrpLeuAlaAlaAspLeuAlaLeuLeuGlyGlu180185190ProThrGlyGlyTrpIleGluAlaGlyCysGlnGlyAsnLeuArgIle195200205LysValThrAlaHisGlyValArgAlaHisSerAlaArgSerTrpLeu210215220GlyAspAsnAlaMetHisLysLeuSerProIleIleSerLysValAla225230235240AlaTyrLysAlaAlaGluValAsnIleAspGlyLeuThrTyrArgGlu245250255GlyLeuAsnIleValPheCysGluSerGlyValAlaAsnAsnValIle260265270ProAspLeuAlaTrpMetAsnLeuAsnPheArgPheAlaProAsnArg275280285AspLeuAsnGluAlaIleGluHisValValGluThrLeuGluLeuAsp290295300GlyGlnAspGlyIleGluTrpAlaValaluAspGlyAlaGlyGlyAla305310315320LeuProGlyLeuGlyGlnGlnValThrSerGlyLeuIleAspAlaVal325330335GlyArgGluLysIleArgAlaLysPheGlyTrpThrAspValSerArg340345350PheSerAlaMetGlyIleProAlaLeuAsnPheGlyAlaGlyAspPro355360365SerPheAlaHisLysArgAspaluGlnCysProValGluGlnIleThr370375380AspValAlaAlaIleLeuLysGlnTyrLeuSerGlu385390395<210>129<211>1107<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1107)<223>RXA02022K<400>129gtgaactctgaactcaaaccaggattagatctcctcggcgacccaatt48MetAsnSerGluLeuLysProGlyLeuAspLeuLeuGlyAspProIle151015gtccttactcaacgtttggtagatataccgagtccgtcgggtcaggaa96ValLeuThrGlnArgLeuValAspIleProSerProSerGlyGlnGlu202530aagcagattgctgatgaaattgaagatgcccttcggaaccttaatcta144LysGlnIleAlaAspGluIleGluAspAlaLeuArgAsnLeuAsnLeu354045cctggtgtagaggtcttccgcttcaacaacaacgttcttgctcgcacg192ProGlyValGluValPheArgPheAsnAsnAsnValLeuAlaArgThr505560aacaggggattggcctcgagggtcatgcttgctggtcatatcgataca240AsnArgGlyLeuAlaSerArgValMetLeuAlaGlyHisIleAspThr65707580gtgccgatcgcggacaatctgccaagccgtgtggaagacggcatcatg288ValProIleAlaAspAsnLeuProSerArgValGluAspGlyIleMet859095tatggctgtggcaccgtcgatatgaaatctgggttggcggtgtatttg336TyrGlyCysGlyThrValAspMetLysSerGlyLeuAlaValTyrLeu100105110catacttttgccaccttggccacgtcgactgagcttaaacatgatctg384HisThrPheAlaThrLeuAlaThrSerThrGluLeuLysHisAspLeu115120125acgctgattgcgtatgagtgcgaggaagttgctgatcacctcaatggt432ThrLeuIleAlaTyrGluCysGluGluValAlaAspHisLeuAsnGly130135140ttgggccacattcgcgatgagcatccggagtggttggcggctgatttg480LeuGlyHisIleArgAspGluHisProGluTrpLeuAlaAlaAspLeu145150155160gcgttgttgggtgagcctactggcggctggattgaggcgggctgccag528AlaLeuLeuGlyGluProThrGlyGlyTrpIleGluAlaGlyCysGln165170175ggcaatctgcgcatcaaggtgacggcgcatggtgtgcgtgcccattcg576GlyAsnLeuArgIleLysValThrAlaHisGlyValArgAlaHisSer180185190gcgagaagctggttgggtgataatgcgatgcataagttgtcgccgatc624AlaArgSerTrpLeuGlyAspAsnAlaMetHisLysLeuSerProIle195200205atttcgaaggttgctgcgtataaggccgcagaagtcaacattgatggc672IleSerLysValAlaAlaTyrLysAlaAlaGluValAsnIleAspGly210215220ttgacctaccgtgaaggcctcaacatcgttttctgcgaatcgggcgtg720LeuThrTyrArgGluGlyLeuAsnIleValPheCysGluSerGlyVal225230235240gcaaacaacgtcattccagacctcgcgtggatgaacctcaacttccgt768AlaAsnAsnValIleProAspLeuAlaTrpMetAsnLeuAsnPheArg245250255ttcgcgccgaatcgcgatctcaacgaggcgatcgagcatgtcgtcgaa816PheAlaProAsnArgAspLeuAsnGluAlaIleGluHisValValGlu260265270acgcttgagcttgacggtcaagacggcatcgaatgggccgtagaagac864ThrLeuGluLeuAspGlyGlnAspGlyIleGluTrpAlaValGluAsp275280285ggggcaggcggtgcccttccaggcttggggcagcaggtgacaagcggg912GlyAlaGlyGlyAlaLeuProGlyLeuGlyGlnGlnValThrSerGly290295300cttatcgacgccgtcggccgcgaaaaaatccgcgcaaaattcggctgg960LeuIleAspAlaValGlyArgGluLysIleArgAlaLysPheGlyTrp305310315320accgatgtctcacgtttttcagccatgggaattccagccctaaacttt1008ThrAspValSerArgPheSerAlaMetGlyIleProAlaLeuAsnPhe325330335ggcgctggtgatccaagtttcgcgcataaacgcgacgagcagtgccca1056GlyAlaGlyAspProSerPheAlaHisLysArgAspGluGlnCysPro340345350gtggagcaaatcacggatgtggcagcaattttgaagcagtacctgagc1104ValGluGlnIleThrAspValAlaAlaIleLeuLysGlnTyrLeuSer355360365gag1107Glu<210>130<211>369<212>PRT<213>谷氨酸棒杆菌<400>130MetAsnSerGluLeuLysProGlyLeuAspLeuLeuGlyAspProIle151015ValLeuThrGlnArgLeuValAspIleProSerProSerGlyGlnGlu202530LysGlnIleAlaAspGluIleGluAspAlaLeuArgAsnLeuAsnLeu354045ProGlyValGluValPheArgPheAsnAsnAsnValLeuAlaArgThr505560AsnArgGlyLeuAlaSerArgValMetLeuAlaGlyHisIleAspThr65707580ValProIleAlaAspAsnLeuProSerArgValGluAspGlyIleMet859095TyrGlyCysGlyThrValAspMetLysSerGlyLeuAlaValTyrLeu100105110HisThrPheAlaThrLeuAlaThrSerThrGluLeuLysHisAspLeu115120125ThrLeuIleAlaTyrGluCysGluGluValAlaAspHisLeuAsnGly130135140LeuGlyHisIleArgAspGluHisProGluTrpLeuAlaAlaAspLeu145150155160AlaLeuLeuGlyGluProThrGlyGlyTrpIleGluAlaGlyCysGln165170175GlyAsnLeuArgIleLysValThrAlaHisGlyValArgAlaHisSer180185190AlaArgSerTrpLeuGlyAspAsnAlaMetHisLysLeuSerProIle195200205IleSerLysValAlaAlaTyrLysAlaAlaGluValAsnIleAspGly210215220LeuThrTyrArgGluGlyLeuAsnIleValPheCysGluSerGlyVal225230235240AlaAsnAsnValIleProAspLeuAlaTrpMetAsnLeuAsnPheArg245250255PheAlaProAsnArgAspLeuAsnGluAlaIleGluHisValValGlu260265270ThrLeuGluLeuAspGlyGlnAspGlyIleGluTrpAlaValGluAsp275280285GlyAlaGlyGlyAlaLeuProGlyLeuGlyGlnGlnValThrSerGly290295300LeuIleAspAlaValGlyArgGluLysIleArgAlaLysPheGlyTrp305310315320ThrAspValSerArgPheSerAlaMetGlyIleProAlaLeuAsnPhe325330335GlyAlaGlyAspProSerPheAlaHisLysArgAspGluGlnCysPro340345350ValGluGlnIleThrAspValAlaAlaIleLeuLysGlnTyrLeuSer355360365Glu<210>131<211>1920<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1920)<223>RXA02139K<400>131atgtgcggccttcttggcatattgactgcaaatgggaacgctgaagca48MetCysGlyLeuLeuGlyIleLeuThrAlaAsnGlyAsnAlaGluAla151015ttcgttcctgcactcgagcgggccttgccatgcatgcgccaccgtggt96PheValProAlaLeuGluArgAlaLeuProCysMetArgHisArgGly202530cctgacgatgccggcacttggcatgacgccgatgcagcgtttggattc144ProAspAspAlaGlyThrTrpHisAspAlaAspAlaAlaPheGlyPhe354045aaccgcctctccatcattgatattgcacactcccaccaaccactgcgt192AsnArgLeuSerIleIleAspIleAlaHisSerHisGlnProLeuArg505560tggggacctgcggatgaacccgaccgctacgcaatgactttcaacggt240TrpGlyProAlaAspGluProAspArgTyrAlaMetThrPheAsnGly65707580gagatctacaactacgttgagctgcgtaaagagctctcggatttggga288GluIleTyrAsnTyrValGluLeuArgLysGluLeuSerAspLeuGly859095tatgcctttaatacttctggcgatggcgagccaattgttgtcggtttc336TyrAlaPheAsnThrSerGlyAspGlyGluProIleValValGlyPhe100105110caccactggggcgagtccgtggtcgagcatctccgcggaatgttcggc384HisHisTrpGlyGluSerValValGluHisLeuArgGlyMetPheGly115120125attgccatttgggatacaaaggaaaagtcgcttttccttgcgcgtgat432IleAlaIleTrpAspThrLysGluLysSerLeuPheLeuAlaArgAsp130135140cagttcggcattaagccactgttctacgcaaccaccgagcatggcacc480GlnPheGlyIleLysProLeuPheTyrAlaThrThrGluHisGlyThr145150155160gtgttctcctcagagaagaagaccatcttggagatggccgaggagatg528ValPheSerSerGluLysLysThrIleLeuGluMetAlaGluGluMet165170175aatctagatctgggccttgataagcgcaccattgagcactacgtggac576AsnLeuAspLeuGlyLeuAspLysArgThrIleGluHisTyrValAsp180185190ttgcagtacgtgcccgagccagatacccttcacgcgcagatttcccgc624LeuGlnTyrValProGluProAspThrLeuHisAlaGlnIleSerArg195200205ttggagtcaggctgcaccgcaacagttcgtccgggcggcaagctggaa672LeuGluSerGlyCysThrAlaThrValArgProGlyGlyLysLeuGlu210215220cagaagcgttacttcaagcctcagttcccagtacagaaggtcgtaaag720GlnLysArgTyrPheLysProGlnPheProValGlnLysValValLys225230235240ggtaaggagcaggacctcttcgatcgcattgcccaggtgttggaggat768GlyLysGluGlnAspLeuPheAspArgIleAlaGlnValLeuGluAsp245250255agcgtcgaaaagcatatgcgtgccgacgtgaccgtaggctcgttcctt816SerValGluLysHisMetArgAlaAspValThrValGlySerPheLeu260265270tccggcggcattgactcaaccgcaattgcggcgcttgcaaagcgccac864SerGlyGlyIleAspSerThrAlaIleAlaAlaLeuAlaLysArgHis275280285aaccctgacctgctcaccttcaccaccggtttcgagcgtgaaggctac912AsnProAspLeuLeuThrPheThrThrGlyPheGluArgGluGlyTyr290295300tcggaggtcgatgtggctgcggagtccgccgctgcgattggcgctgag960SerGluValAspValAlaAlaGluSerAlaAlaAlaIleGlyAlaGlu305310315320cacatcgtgaagattgtctcgcctgaggaatacgccaacgcgattcct1008HisIleValLysIleValSerProGluGluTyrAlaAsnAlaIlePro325330335aagatcatgtggtacttggatgatcctgtagctgacccatcattggtc1056LysIleMetTrpTyrLeuAspAspProValAlaAspProSerLeuVal340345350ccgctgtacttcgtggcagcggaagcacgtaagcacgtcaaggttgtg1104ProLeuTyrPheValAlaAlaGluAlaArgLysHisValLysValVal355360365ctgtctggcgagggcgcagatgagctgttcggtggatacaccatttac1152LeuSerGlyGluGlyAlaAspGluLeuPheGlyGlyTyrThrIleTyr370375380aaggagccgctatcgcttgctccatttgagaagatcccttccccacta1200LysGluProLeuSerLeuAlaProPheGluLysIleProSerProLeu385390395400cgtaaaggcctgggaaagctcagcaaggttctgccagacggcatgaag1248ArgLysGlyLeuGlyLysLeuSerLysValLeuProAspGlyMetLys405410415ggcaagtcccttcttgagcgtggctccatgaccatggaagagcgctac1296GlyLysSerLeuLeuGluArgGlySerMetThrMetGluGluArgTyr420425430tacggcaacgctcgctccttcaatttcgagcagatgcaacgcgttatt1344TyrGlyAsnAlaArgSerPheAsnPheGluGlnMetGlnArgValIle435440445ccatgggcaaagcgcgaatgggaccaccgcgaagtcactgcgccgatc1392ProTrpAlaLysArgGluTrpAspHisArgGluValThrAlaProIle450455460tacgcacagtcccgcaactttgatccagtagcccgcatgcaacacctg1440TyrAlaGlnSerArgAsnPheAspProValAlaArgMetGlnHisLeu465470475480gatctgttcacctggatgcgcggcgacatcctggtcaaggctgacaag1488AspLeuPheThrTrpMetArgGlyAspIleLeuValLysAlaAspLys485490495atcaacatggcgaactcccttgagctgcgagttccattcttggataag1536IleAsnMetAlaAsnSerLeuGluLeuArgValProPheLeuAspLys500505510gaagttttcaaggttgcagagaccattccttacgacctgaagattgcc1584GluValPheLysValAlaGluThrIleProTyrAspLeuLysIleAla515520525aacggtaccaccaagtacgcgctgcgcagggcactcgagcagattgtt1632AsnGlyThrThrLysTyrAlaLeuArgArgAlaLeuGluGlnIleVal530535540ccgcctcacgttttgcaccgcaagaagctgggcttccctgttcccatg1680ProProHisValLeuHisArgLysLysLeuGlyPheProValProMet545550555560cgccactggcttgccggcgatgagctgttcggttgggcgcaggacacc1728ArgHisTrpLeuAlaGlyAspGluLeuPheGlyTrpAlaGlnAspThr565570575atcaaggaatccggtactgaagatatcttcaacaagcaggctgtgctg1776IleLysGluSerGlyThrGluAspIlePheAsnLysGlnAlaValLeu580585590gatatgctgaacgagcaccgcgatggcgtgtcagatcattcccgtcga1824AspMetLeuAsnGluHisArgAspGlyValSerAspHisSerArgArg595600605ctgtggactgttctgtcatttatggtgtggcacggcatttttgtggaa1872LeuTrpThrValLeuSerPheMetValTrpHisGlyIlePheValGlu610615620aaccgcattgatccacagattgaggaccgctcctacccagtcgagctt1920AsnArgIleAspProGlnIleGluAspArgSerTyrProValGluLeu625630635640<210>132<211>640<212>PRT<213>谷氨酸棒杆菌<400>132MetCysGlyLeuLeuGlyIleLeuThrAlaAsnGlyAsnAlaGluAla151015PheValProAlaLeuGluArgAlaLeuProCysMetArgHisArgGly202530ProAspAspAlaGlyThrTrpHisAspAlaAspAlaAlaPheGlyPhe354045AsnArgLeuSerIleIleAspIleAlaHisSerHisGlnProLeuAra505560TrpGlyProAlaAspGluProAspArgTyrAlaMetThrPheAsnGly65707580GluIleTyrAsnTyrValGluLeuArgLysGluLeuSerAspLeuGly859095TyrAlaPheAsnThrSerGlyAspGlyGluProIleValValGlyPhe100105110HisHisTrpGlyGluSerValValGluHisLeuArgGlyMetPheGly115120125IleAlaIleTrpAspThrLysGluLysSerLeuPheLeuAlaArgAsp130135140GlnPheGlyIleLysProLeuPheTyrAlaThrThrGluHisGlyThr145150155160ValPheSerSerGluLysLysThrIleLeuGluMetAlaGluGluMet165170175AsnLeuAspLeuGlyLeuAspLysArgThrIleGluHisTyrValAsp180185190LeuGlnTyrValProGluProAspThrLeuHisAlaGlnIleSerArg195200205LeuGluSerGlyCysThrAlaThrValArgProGlyGlyLysLeuGlu210215220GlnLysArgTyrPheLysProGlnPheProValGlnLysValValLys225230235240GlyLysGluGlnAspLeuPheAspArgIleAlaGlnValLeuGluAsp245250255SerValGluLysHisMetArgAlaAspValThrValGlySerPheLeu260265270SerGlyGlyIleAspSerThrAlaIleAlaAlaLeuAlaLysArgHis275280285AsnProAspLeuLeuThrPheThrThrGlyPheGluArgGluGlyTyr290295300SerGluValAspValAlaAlaGluSerAlaAlaAlaIleGlyAlaGlu305310315320HisIleValLysIleValSerProGluGluTyrAlaAsnAlaIlePro325330335LysIleMetTrpTyrLeuAspAspProValAlaAspProSerLeuVal340345350ProLeuTyrPheValAlaAlaGluAlaArgLysHisValLysValVal355360365LeuSerGlyGluGlyAlaAspGluLeuPheGlyGlyTyrThrIleTyr370375380LysGluProLeuSerLeuAlaProPheGluLysIleProSerProLeu385390395400ArgLysGlyLeuGlyLysLeuSerLysValLeuProAspGlyMetLys405410415GlyLysSerLeuLeuGluArgGlySerMetThrMetGluGluArgTyr420425430TyrGlyAsnAlaArgSerPheAsnPheGluGlnMetGlnArgValIle435440445ProTrpAlaLysArgGluTrpAspHisArgGluValThrAlaProIle450455460TyrAlaGlnSerArgAsnPheAspProValAlaArgMetGlnHisLeu465470475480AspLeuPheThrTrpMetArgGlyAspIleLeuValLysAlaAspLys485490495IleAsnMetAlaAsnSerLeuGluLeuArgValProPheLeuAspLys500505510GluValPheLysValAlaGluThrIleProTyrAspLeuLysIleAla515520525AsnGlyThrThrLysTyrAlaLeuArgArgAlaLeuGluGlnIleVal530535540ProProHisValLeuHisArgLysLysLeuGlyPheProValProMet545550555560ArgHisTrpLeuAlaGlyAspGluLeuPheGlyTrpAlaGlnAspThr565570575IleLysGluSerGlyThrGluAspIlePheAsnLysGlnAlaValLeu580585590AspMetLeuAsnGluHisArgAspGlyValSerAspHisSerArgArg595600605LeuTrpThrValLeuSerPheMetValTrpHisGlyIlePheValGlu610615620AsnArgIleAspProGlnIleGluAspArgSerTyrProValGluLeu625630635640<210>133<211>1173<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1173)<223>RXA02157W<400>133atgagcacgctggaaacttggccacaggtcattattaatacgtacggc48MetSerThrLeuGluThrTrpProGlnValIleIleAsnThrTyrGly151015accccaccagttgagctggtgtccggcaagggcgcaaccgtcactgat96ThrProProValGluLeuValSerGlyLysGlyAlaThrValThrAsp202530gaccagggcaatgtctacatcgacttgctcgcgggcatcgcagtcaac144AspGlnGlyAsnValTyrIleAspLeuLeuAlaGlyIleAlaValAsn354045gcgttgggccacgcccacccggcgatcatcgaggcggtcaccaaccag192AlaLeuGlyHisAlaHisProAlaIleIleGluAlaValThrAsnGln505560atcggccaacttggtcacgtctcaaacttgttcgcatccaggcccgtc240IleGlyGlnLeuGlyHisValSerAsnLeuPheAlaSerArgProVal65707580gtcgaggtcgccgaggagctcatcaagcgtttttcgcttgacgacgcc288ValGluValAlaGluGluLeuIleLysArgPheSerLeuAspAspAla859095accctcgccgcgcaaacccgggttttcttctgcaactcgggcgccgaa336ThrLeuAlaAlaGlnThrArgValPhePheCysAsnSerGlyAlaGlu100105110gcaaacgaggctgctttcaagattgcacgcttgactggtcgttcccgg384AlaAsnGluAlaAlaPheLysIleAlaArgLeuThrGlyArgSerArg115120125attctggctgcagttcatggtttccacggccgcaccatgggttccctc432IleLeuAlaAlaValHisGlyPheHisGlyArgThrMetGlySerLeu130135140gcgctgactggccagccagacaagcgtgaagcgttcctgccaatgcca480AlaLeuThrGlyGlnProAspLysArgGluAlaPheLeuProMetPro145150155160agcggtgtggagttctacccttacggcgacaccgattacttgcgcaaa528SerGlyValGluPheTyrProTyrGlyAspThrAspTyrLeuArgLys165170175atggtagaaaccaacccaacggatgtggctgctatcttcctcgagcca576MetValGluThrAsnProThrAspValAlaAlaIlePheLeuGluPro180185190atccagggtgaaacgggcgttgttccagcacctgaaggattcctcaag624IleGlnGlyGluThrGlyValValProAlaProGluGlyPheLeuLys195200205gcagtgcgcgagctgtgcgatgagtacggcatcttgatgatcaccgat672AlaValArgGluLeuCysAspGluTyrGlyIleLeuMetIleThrAsp210215220gaagtccagactggcgttggccgtaccggcgatttctttgcacatcag720GluValGlnThrGlyValGlyArgThrGlyAspPhePheAlaHisGln225230235240cacgatggcgttgttcccgatgtggtgaccatggccaagggacttggc768HisAspGlyValValProAspValValThrMetAlaLysGlyLeuGly245250255ggcggtcttcccatcggtgcttgtttggccactggccgtgcagctgaa816GlyGlyLeuProIleGlyAlaCysLeuAlaThrGlyArgAlaAlaGlu260265270ttgatgaccccaggcaagcacggcaccactttcggtggcaacccagtt864LeuMetThrProGlyLysHisGlyThrThrPheGlyGlyAsnProVal275280285gcttgtgcagctgccaaggcagtgctgtctgttgtcgatgacgctttc912AlaCysAlaAlaAlaLysAlaValLeuSerValValAspAspAlaPhe290295300tgcgcagaagttgcccgcaagggcgagctgttcaaggaacttcttgcc960CysAlaGluValAlaArgLysGlyGluLeuPheLysGluLeuLeuAla305310315320aaggttgacggcgttgtagacgtccgtggcaggggcttgatgttgggc1008LysValAspGlyValValAspValArgGlyArgGlyLeuMetLeuGly325330335gtggtgctggagcgcgacgtcgcaaagcaagctgttcttgatggtttt1056ValValLeuGluArgAspValAlaLysGlnAlaValLeuAspGlyPhe340345350aagcacggcgttattttgaatgcaccggcggacaacattatccgtttg1104LysHisGlyValIleLeuAsnAlaProAlaAspAsnIleIleArgLeu355360365accccgccgctggtgatcaccgacgaagaaatcgcagacgcagtcaag1152ThrProProLeuValIleThrAspGluGluIleAlaAspAlaValLys370375380gctattgccgagacaatcgca1173AlaIleAlaGluThrIleAla385390<210>134<211>391<212>PRT<213>谷氨酸棒杆菌<400>134MetSerThrLeuGluThrTrpProGlnValIleIleAsnThrTyrGly151015ThrProProValGluLeuValSerGlyLysGlyAlaThrValThrAsp202530AspGlnGlyAsnValTyrIleAspLeuLeuAlaGlyIleAlaValAsn354045AlaLeuGlyHisAlaHisProAlaIleIleGluAlaValThrAsnGln505560IleGlyGlnLeuGlyHisValSerAsnLeuPheAlaSerArgProVal65707580ValGluValAlaGluGluLeuIleLysArgPheSerLeuAspAspAla859095ThrLeuAlaAlaGlnThrArgValPhePheCysAsnSerGlyAlaGlu100105110AlaAsnGluAlaAlaPheLysIleAlaArgLeuThrGlyArgSerArg115120125IleLeuAlaAlaValHisGlyPheHisGlyArgThrMetGlySerLeu130135140AlaLeuThrGlyGlnProAspLysArgGluAlaPheLeuProMetPro145150155160SerGlyValGluPheTyrProTyrGlyAspThrAspTyrLeuArgLys165170175MetValGluThrAsnProThrAspValAlaAlaIlePheLeuGluPro180185190IleGlnGlyGluThrGlyValValProAlaProGluGlyPheLeuLys195200205AlaValArgGluLeuCysAspGluTyrGlyIleLeuMetIleThrAsp210215220GluValGlnThrGlyValGlyArgThrGlyAspPhePheAlaHisGln225230235240HisAspGlyValValProAspValValThrMetAlaLysGlyLeuGly245250255GlyGlyLeuProIleGlyAlaCysLeuAlaThrGlyArgAlaAlaGlu260265270LeuMetThrProGlyLysHisGlyThrThrPheGlyGlyAsnProVal275280285AlaCysAlaAlaAlaLysAlaValLeuSerValValAspAspAlaPhe290295300CysAlaGluValAlaArgLysGlyGluLeuPheLysGluLeuLeuAla305310315320LysValAspGlyValValAspValArgGlyArgGlyLeuMetLeuGly325330335ValValLeuGluArgAspValAlaLysGlnAlaValLeuAspGlyPhe340345350LysHisGlyValIleLeuAsnAlaProAlaAspAsnIleIleArgLeu355360365ThrProProLeuValIleThrAspGluGluIleAlaAspAlaValLys370375380AlaIleAlaGluThrIleAla385390<210>135<211>831<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(831)<223>RXA02229K<400>135gtgaatttgaccatcccctttgccaaaggccacgccaccgaaaacgac48MetAsnLeuThrIleProPheAlaLysGlyHisAlaThrGluAsnAsp151015ttcatcatcatccccgatgaggatgcgcgcctagatttaactccagaa96PheIleIleIleProAspGluAspAlaArgLeuAspLeuThrProGlu202530atggtggtcacgctgtgtgaccgccgcgccgggatcggtgctgatggt144MetValValThrLeuCysAspArgArgAlaGlyIleGlyAlaAspGly354045atcctccgcgtggttaaagctgcagacgtagaaggctccacggtcgac192IleLeuArgValValLysAlaAlaAspValGluGlySerThrValAsp505560ccatcgctgtggttcatggattaccgcaacgccgatggatctttggct240ProSerLeuTrpPheMetAspTyrArgAsnAlaAspGlySerLeuAla65707580gaaatgtgcggcaatggtgtgcgcctgttcgcgcactggctgtactcc288GluMetCysGlyAsnGlyValArgLeuPheAlaHisTrpLeuTyrSer859095cgcggtcttgttgataatacgagctttgatatcggtacccgcgccggt336ArgGlyLeuValAspAsnThrSerPheAspIleGlyThrArgAlaGly100105110gtccgccacgttgatattttgcaggcagatcaacattctgcgcaggtc384ValArgHisValAspIleLeuGlnAlaAspGlnHisSerAlaGlnVal115120125cgcgttgatatgggcatccctgacgtcacgggattatccacctgcgac432ArgValAspMetGlyIleProAspValThrGlyLeuSerThrCysAsp130135140atcaacggccaagtattcgctggccttggcgttgatatgggtaaccca480IleAsnGlyGlnValPheAlaGlyLeuGlyValAspMetGlyAsnPro145150155160cacctagcgtgcgttgtgccgggcttaagtgcgtcggctcttgccgat528HisLeuAlaCysValValProGlyLeuSerAlaSerAlaLeuAlaAsp165170175atggaactgcgcgcacctacgtttgatcaggaattcttcccccacggt576MetGluLeuArgAlaProThrPheAspGlnGluPhePheProHisGly180185190gtgaacgtagaaatcgtcacagaattagaagatgacgcagtatcgatg624ValAsnValGluIleValThrGluLeuGluAspAspAlaValSerMet195200205cgcgtgtgggaacgcggagtgggcgaaacccgctcctgtggcacggga672ArgValTrpGluArgGlyValGlyGluThrArgSerCysGlyThrGly210215220accgttgctgcagcgtgtgctgctttagctgatgctggattgggagaa720ThrValAlaAlaAlaCysAlaAlaLeuAlaAspAlaGlyLeuGlyGlu225230235240ggcacagttaaagtgtgcgttccaggtggggaagtagaagtccagatc768GlyThrValLysValCysValProGlyGlyGluValGluValGlnIle245250255tttgacgacggctccacactcaccggcccaagcgccatcatcgcactc816PheAspAspGlySerThrLeuThrGlyProSerAlaIleIleAlaLeu260265270ggtgaggtgcagatc831GlyGluValGlnIle275<210>136<211>277<212>PRT<213>谷氨酸棒杆菌<400>136MetAsnLeuThrIleProPheAlaLysGlyHisAlaThrGluAsnAsp151015PheIleIleIleProAspGluAspAlaArgLeuAspLeuThrProGlu202530MetValValThrLeuCysAspArgArgAlaGlyIleGlyAlaAspGly354045IleLeuArgValValLysAlaAlaAspValGluGlySerThrValAsp505560ProSerLeuTrpPheMetAspTyrArgAsnAlaAspGlySerLeuAla65707580GluMetCysGlyAsnGlyValArgLeuPheAlaHisTrpLeuTyrSer859095ArgGlyLeuValAspAsnThrSerPheAspIleGlyThrArgAlaGly100105110ValArgHisValAspIleLeuGlnAlaAspGlnHisSerAlaGlnVal115120125ArgValAspMetGlyIleProAspValThrGlyLeuSerThrCysAsp130135140IleAsnGlyGlnValPheAlaGlyLeuGlyValAspMetGlyAsnPro145150155160HisLeuAlaCysValValProGlyLeuSerAlaSerAlaLeuAlaAsp165170175MetGluLeuArgAlaProThrPheAspGlnGluPhePheProHisGly180185190ValAsnValGluIleValThrGluLeuGluAspAspAlaValSerMet195200205ArgValTrpGluArgGlyValGlyGluThrArgSerCysGlyThrGly210215220ThrValAlaAlaAlaCysAlaAlaLeuAlaAspAlaGlyLeuGlyGlu225230235240GlyThrValLysValCysValProGlyGlyGluValGluValGlnIle245250255PheAspAspGlySerThrLeuThrGlyProSerAlaIleIleAlaLeu260265270GlyGluValGlnIle275<210>137<211>2823<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2823)<223>RXA02259E<400>137ttgctgggtgattttttatctcatgcacgccaacaccctcaatgtgaa48MetLeuGlyAspPheLeuSerHisAlaArgGlnHisProGlnCysGlu151015agagtgtttaaagtagttatgactgattttttacgcgatgacatcagg96ArgValPheLysValValMetThrAspPheLeuArgAspAspIleArg202530ttcctcggtcaaatcctcggtgaggtaattgcggaacaagaaggccag144PheLeuGlyGlnIleLeuGlyGluValIleAlaGluGlnGluGlyGln354045gaggtttatgaactggtcgaacaagcgcgcctgacttcttttgatatc192GluValTyrGluLeuValGluGlnAlaArgLeuThrSerPheAspIle505560gccaagggcaacgccgaaatggatagcctggttcaggttttcgacggc240AlaLysGlyAsnAlaGluMetAspSerLeuValGlnValPheAspGly65707580attactccagccaaggcaacaccgattgctcgcgcattttcccacttc288IleThrProAlaLysAlaThrProIleAlaArgAlaPheSerHisPhe859095gctctgctggctaacctggcggaagacctctacgatgaagagcttcgt336AlaLeuLeuAlaAsnLeuAlaGluAspLeuTyrAspGluGluLeuArg100105110gaacaggctctcgatgcaggcgacacccctccggacagcactcttgat384GluGlnAlaLeuAspAlaGlyAspThrProProAspSerThrLeuAsp115120125gccacctggctgaaactcaatgagggcaatgttggcgcagaagctgtg432AlaThrTrpLeuLysLeuAsnGluGlyAsnValGlyAlaGluAlaVal130135140gccgatgtgctgcgcaatgctgaggtggcgccggttctgactgcgcac480AlaAspValLeuArgAsnAlaGluValAlaProValLeuThrAlaHis145150155160ccaactgagactcgccgccgcactgtttttgatgcgcaaaagtggatc528ProThrGluThrArgArgArgThrValPheAspAlaGlnLysTrpIle165170175accacccacatgcgtgaacgccacgctttgcagtctgcggagcctacc576ThrThrHisMetArgGluArgHisAlaLeuGlnSerAlaGluProThr180185190gctcgtacgcaaagcaagttggatgagatcgagaagaacatccgccgt624AlaArgThrGlnSerLysLeuAspGluIleGluLysAsnIleArgArg195200205cgcatcaccattttgtggcagaccgcgttgattcgtgtggcccgccca672ArgIleThrIleLeuTrpGlnThrAlaLeuIleArgValAlaArgPro210215220cgtatcgaggacgagatcgaagtagggctgcgctactacaagctgagc720ArgIleGluAspGluIleGluValGlyLeuArgTyrTyrLysLeuSer225230235240cttttggaagagattccacgtatcaaccgtgatgtggctgttgagctt768LeuLeuGluGluIleProArgIleAsnArgAspValAlaValGluLeu245250255cgtgagcgtttcggcgagggtgttcctttgaagcccgtggtcaagcca816ArgGluArgPheGlyGluGlyValProLeuLysProValValLysPro260265270ggttcctggattggtggagaccacgacggtaacccttatgtcaccgcg864GlySerTrpIleGlyGlyAspHisAspGlyAsnProTyrValThrAla275280285gaaacagttgagtattccactcaccgcgctgcggaaaccgtgctcaag912GluThrValGluTyrSerThrHisArgAlaAlaGluThrValLeuLys290295300tactatgcacgccagctgcattccctcgagcatgagctcagcctgtcg960TyrTyrAlaArgGlnLeuHisSerLeuGluHisGluLeuSerLeuSer305310315320gaccgcatgaataaggtcaccccgcagctgcttgcgctggcagatgca1008AspArgMetAsnLysValThrProGlnLeuLeuAlaLeuAlaAspAla325330335gggcacaacgacgtgccaagccgcgtggatgagccttatcgacgcgcc1056GlyHisAsnAspValProSerArgValAspGluProTyrArgArgAla340345350gtccatggcgttcgcggacgtatcctcgcgacgacggccgagctgatc1104ValHisGlyValArgGlyArgIleLeuAlaThrThrAlaGluLeuIle355360365ggcgaggacgccgttgagggcgtgtggttcaaggtctttactccatac1152GlyGluAspAlaValGluGlyValTrpPheLysValPheThrProTyr370375380gcatctccggaagaattcttaaacgatgcgttgaccattgatcattct1200AlaSerProGluGluPheLeuAsnAspAlaLeuThrIleAspHisSer385390395400ctgcgtgaatccaaggacgttctcattgccgatgatcgtttgtctgtg1248LeuArgGluSerLysAspValLeuIleAlaAspAspArgLeuSerVal405410415ctgatttctgccatcgagagctttggattcaacctttacgcactggat1296LeuIleSerAlaIleGluSerPheGlyPheAsnLeuTyrAlaLeuAsp420425430ctgcgccaaaactccgaaagctacgaggacgtcctcaccgagcttttc1344LeuArgGlnAsnSerGluSerTyrGluAspValLeuThrGluLeuPhe435440445gaacgcgcccaagtcaccgcaaactaccgcgagctgtctgaagcagag1392GluArgAlaGlnValThrAlaAsnTyrArgGluLeuSerGluAlaGlu450455460aagcttgaggtgctgctgaaggaactgcgcagccctcgtccgctgatc1440LysLeuGluValLeuLeuLysGluLeuArgSerProArgProLeuIle465470475480ccgcacggttcagatgaatacagcgaggtcaccgaccgcgagctcggc1488ProHisGlySerAspGluTyrSerGluValThrAspArgGluLeuGly485490495atcttccgcaccgcgtcggaggctgttaagaaattcgggccacggatg1536IlePheArgThrAlaSerGluAlaValLysLysPheGlyProArgMet500505510gtgcctcactgcatcatctccatggcatcatcggtcaccgatgtgctc1584ValProHisCysIleIleSerMetAlaSerSerValThrAspValLeu515520525gagccgatggtgttgctcaaggaattcggactcatcgcagccaacggc1632GluProMetValLeuLeuLysGluPheGlyLeuIleAlaAlaAsnGly530535540gacaacccacgcggcaccgtcgatgtcatcccactgttcgaaaccatc1680AspAsnProArgGlyThrValAspValIleProLeuPheGluThrIle545550555560gaagatctccaggccggcgccggaatcctcgacgaactgtggaaaatt1728GluAspLeuGlnAlaGlyAlaGlyIleLeuAspGluLeuTrpLysIle565570575gatctctaccgcaactacctcctgcagcgcgacaacgtccaggaagtc1776AspLeuTyrArgAsnTyrLeuLeuGlnArgAspAsnValGlnGluVal580585590atgctcggttactccgattccaacaaggatggcggatatttctccgca1824MetLeuGlyTyrSerAspSerAsnLysAspGlyGlyTyrPheSerAla595600605aactgggcgctttacgacgcggaactgcagctcgtcgaactatgccga1872AsnTrpAlaLeuTyrAspAlaGluLeuGlnLeuValGluLeuCysArg610615620tcagccggggtcaagcttcgcctgttccacggccgtggtggcaccgtc1920SerAlaGlyValLysLeuArgLeuPheHisGlyArgGlyGlyThrVal625630635640ggccgcggtggcggaccttcctacgacgcgattcttgcccagcccagg1968GlyArgGlyGlyGlyProSerTyrAspAlaIleLeuAlaGlnProArg645650655ggggctgtccaaggttccgtgcgcatcaccgagcagggcgagatcatc2016GlyAlaValGlnGlySerValArgIleThrGluGlnGlyGluIleIle660665670tccgctaagtacggcaaccccgaaaccgcgcgccgaaacctcgaagcc2064SerAlaLysTyrGlyAsnProGluThrAlaArgArgAsnLeuGluAla675680685ctggtctcagccacgcttgaggcatcgcttctcgacgtctccgaactc2112LeuValSerAlaThrLeuGluAlaSerLeuLeuAspValSerGluLeu690695700accgatcaccaacgcgcgtacgacatcatgagtgagatctctgagctc2160ThrAspHisGlnArgAlaTyrAspIleMetSerGluIleSerGluLeu705710715720agcttgaagaagtacgcctccttggtgcacgaggatcaaggcttcatc2208SerLeuLysLysTyrAlaSerLeuValHisGluAspGlnGlyPheIle725730735gattacttcacccagtccacgccgctgcaggagattggatccctcaac2256AspTyrPheThrGlnSerThrProLeuGlnGluIleGlySerLeuAsn740745750atcggatccaggccttcctcacgcaagcagacctcctcggtggaagat2304IleGlySerArgProSerSerArgLysGlnThrSerSerValGluAsp755760765ttgcgagccatcccatgggtgctcagctggtcacagtctcgtgtcatg2352LeuArgAlaIleProTrpValLeuSerTrpSerGlnSerArgValMet770775780ctgccaggctggtttggtgtcggaaccgcattagagcagtggattggc2400LeuProGlyTrpPheGlyValGlyThrAlaLeuGluGlnTrpIleGly785790795800gaaggggagcaggccacccaacgcattgccgagctgcaaacactcaat2448GluGlyGluGlnAlaThrGlnArgIleAlaGluLeuGlnThrLeuAsn805810815gagtcctggccatttttcacctcagtgttggataacatggctcaggtg2496GluSerTrpProPhePheThrSerValLeuAspAsnMetAlaGlnVal820825830atgtccaaggcagagctgcgtttggcaaagctctacgcagacctgatc2544MetSerLysAlaGluLeuArgLeuAlaLysLeuTyrAlaAspLeuIle835840845ccagatacggaagtagccgagcgagtctattccgtcatccgcgaggag2592ProAspThrGluValAlaGluArgValTyrSerValIleArgGluGlu850855860tacttcctgaccaagaagatgttctgcgtaatcaccggctctgatgat2640TyrPheLeuThrLysLysMetPheCysValIleThrGlySerAspAsp865870875880ctgcttgatgacaacccacttctcgcacgctctgtccagcgccgatac2688LeuLeuAspAspAsnProLeuLeuAlaArgSerValGlnArgArgTyr885890895ccctacctgcttccactcaacgtgatccaggtagagatgatgcgacgc2736ProTyrLeuLeuProLeuAsnValIleGlnValGluMetMetArgArg900905910taccgaaaaggcgaccaaagcgagcaagtgtcccgcaacattcagctg2784TyrArgLysGlyAspGlnSerGluGlnValSerArgAsnIleGlnLeu915920925accatgaacggtctttccactgcgctgcgcaactccggc2823ThrMetAsnGlyLeuSerThrAlaLeuArgAsnSerGly930935940<210>138<211>941<212>PRT<213>谷氨酸棒杆菌<400>138MetLeuGlyAspPheLeuSerHisAlaArgGlnHisProGlnCysGlu151015ArgValPheLysValValMetThrAspPheLeuArgAspAspIleArg202530PheLeuGlyGlnIleLeuGlyGluValIleAlaGluGlnGluGlyGln354045GluValTyrGluLeuValGluGlnAlaArgLeuThrSerPheAspIle505560AlaLysGlyAsnAlaGluMetAspSerLeuValGlnValPheAspGly65707580IleThrProAlaLysAlaThrProIleAlaArgAlaPheSerHisPhe859095AlaLeuLeuAlaAsnLeuAlaGluAspLeuTyrAspGluGluLeuArg100105110GluGlnAlaLeuAspAlaGlyAspThrProProAspSerThrLeuAsp115120125AlaThrTrpLeuLysLeuAsnGluGlyAsnValGlyAlaGluAlaVal130135140AlaAspValLeuArgAsnAlaGluValAlaProValLeuThrAlaHis145150155160ProThrGluThrArgArgArgThrValPheAspAlaGlnLysTrpIle165170175ThrThrHisMetArgGluArgHisAlaLeuGlnSerAlaGluProThr180185190AlaArgThrGlnSerLysLeuAspGluIleGluLysAsnIleArgArg195200205ArgIleThrIleLeuTrpGlnThrAlaLeuIleArgValAlaArgPro210215220ArgIleGluAspGluIleGluValGlyLeuArgTyrTyrLysLeuSer225230235240LeuLeuGluGluIleProArgIleAsnArgAspValAlaValGluLeu245250255ArgGluArgPheGlyGluGlyValProLeuLysProValValLysPro260265270GlySerTrpIleGlyGlyAspHisAspGlyAsnProTyrValThrAla275280285GluThrValGluTyrSerThrHisArgAlaAlaGluThrValLeuLys290295300TyrTyrAlaArgGlnLeuHisSerLeuGluHisGluLeuSerLeuSer305310315320AspArgMetAsnLysValThrProGlnLeuLeuAlaLeuAlaAspAla325330335GlyHisAsnAspValProSerArgValAspGluProTyrArgArgAla340345350ValHisGlyValArgGlyArgIleLeuAlaThrThrAlaGluLeuIle355360365GlyGluAspAlaValGluGlyValTrpPheLysValPheThrProTyr370375380AlaSerProGluGluPheLeuAsnAspAlaLeuThrIleAspHisSer385390395400LeuArgGluSerLysAspValLeuIleAlaAspAspArgLeuSerVal405410415LeuIleSerAlaIleGluSerPheGlyPheAsnLeuTyrAlaLeuAsp420425430LeuArgGlnAsnSerGluSerTyrGluAspValLeuThrGluLeuPhe435440445GluArgAlaGlnValThrAlaAsnTyrArgGluLeuSerGluAlaGlu450455460LysLeuGluValLeuLeuLysGluLeuArgSerProArgProLeuIle465470475480ProHisGlySerAspGluTyrSerGluValThrAspArgGluLeuGly485490495IlePheArgThrAlaSerGluAlaValLysLysPheGlyProArgMet500505510ValProHisCysIleIleSerMetAlaSerSerValThrAspValLeu515520525GluProMetValLeuLeuLysGluPheGlyLeuIleAlaAlaAsnGly530535540AspAsnProArgGlyThrValAspValIleProLeuPheGluThrIle545550555560GluAspLeuGlnAlaGlyAlaGlyIleLeuAspGluLeuTrpLysIle565570575AspLeuTyrArgAsnTyrLeuLeuGlnArgAspAsnValGlnGluVal580585590MetLeuGlyTyrSerAspSerAsnLysAspGlyGlyTyrPheSerAla595600605AsnTrpAlaLeuTyrAspAlaGluLeuGlnLeuValGluLeuCysArg610615620SerAlaGlyValLysLeuArgLeuPheHisGlyArgGlyGlyThrVal625630635640GlyArgGlyGlyGlyProSerTyrAspAlaIleLeuAlaGlnProArg645650655GlyAlaValGlnGlySerValArgIleThrGluGlnGlyGluIleIle660665670SerAlaLysTyrGlyAsnProGluThrAlaArgArgAsnLeuGluAla675680685LeuValSerAlaThrLeuGluAlaSerLeuLeuAspValSerGluLeu690695700ThrAspHisGlnArgAlaTyrAspIleMetSerGluIleSerGluLeu705710715720SerLeuLysLysTyrAlaSerLeuValHisGluAspGlnGlyPheIle725730735AspTyrPheThrGlnSerThrProLeuGlnGluIleGlySerLeuAsn740745750IleGlySerArgProSerSerArgLysGlnThrSerSerValGluAsp755760765LeuArgAlaIleProTrpValLeuSerTrpSerGlnSerArgValMet770775780LeuProGlyTrpPheGlyValGlyThrAlaLeuGluGlnTrpIleGly785790795800GluGlyGluGlnAlaThrGlnArgIleAlaGluLeuGlnThrLeuAsn805810815GluSerTrpProPhePheThrSerValLeuAspAsnMetAlaGlnVal820825830MetSerLysAlaGluLeuArgLeuAlaLysLeuTyrAlaAspLeuIle835840845ProAspThrGluValAlaGluArgValTyrSerValIleArgGluGlu850855860TyrPheLeuThrLysLysMetPheCysValIleThrGlySerAspAsp865870875880LeuLeuAspAspAsnProLeuLeuAlaArgSerValGlnArgArgTyr885890895ProTyrLeuLeuProLeuAsnValIleGlnValGluMetMetArgArg900905910TyrArgLysGlyAspGlnSerGluGlnValSerArgAsnIleGlnLeu915920925ThrMetAsnGlyLeuSerThrAlaLeuArgAsnSerGly930935940<210>139<211>2757<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2757)<223>RXA02259K<400>139atgactgattttttacgcgatgacatcaggttcctcggtcaaatcctc48MetThrAspPheLeuArgAspAspIleArgPheLeuGlyGlnIleLeu151015ggtgaggtaattgcggaacaagaaggccaggaggtttatgaactggtc96GlyGluValIleAlaGluGlnGluGlyGlnGluValTyrGluLeuVal202530gaacaagcgcgcctgacttcttttgatatcgccaagggcaacgccgaa144GluGlnAlaArgLeuThrSerPheAspIleAlaLysGlyAsnAlaGlu354045atggatagcctggttcaggttttcgacggcattactccagccaaggca192MetAspSerLeuValGlnValPheAspGlyIleThrProAlaLysAla505560acaccgattgctcgcgcattttcccacttcgctctgctggctaacctg240ThrProIleAlaArgAlaPheSerHisPheAlaLeuLeuAlaAsnLeu65707580gcggaagacctctacgatgaagagcttcgtgaacaggctctcgatgca288AlaGluAspLeuTyrAspGluGluLeuArgGluGlnAlaLeuAspAla859095ggcgacacccctccggacagcactcttgatgccacctggctgaaactc336GlyAspThrProProAspSerThrLeuAspAlaThrTrpLeuLysLeu100105110aatgagggcaatgttggcgcagaagctgtggccgatgtgctgcgcaat384AsnGluGlyAsnValGlyAlaGluAlaValAlaAspValLeuArgAsn115120125gctgaggtggcgccggttctgactgcgcacccaactgagactcgccgc432AlaGluValAlaProValLeuThrAlaHisProThrGluThrArgArg130135140cgcactgtttttgatgcgcaaaagtggatcaccacccacatgcgtgaa480ArgThrValPheAspAlaGlnLysTrpIleThrThrHisMetArgGlu145150155160cgccacgctttgcagtctgcggagcctaccgctcgtacgcaaagcaag528ArgHisAlaLeuGlnSerAlaGluProThrAlaArgThrGlnSerLys165170175ttggatgagatcgagaagaacatccgccgtcgcatcaccattttgtgg576LeuAspGluIleGluLysAsnIleArgArgArgIleThrIleLeuTrp180185190cagaccgcgttgattcgtgtggcccgcccacgtatcgaggacgagatc624GlnThrAlaLeuIleArgValAlaArgProArgIleGluAspGluIle195200205gaagtagggctgcgctactacaagctgagccttttggaagagattcca672GluValGlyLeuArgTyrTyrLysLeuSerLeuLeuGluGluIlePro210215220cgtatcaaccgtgatgtggctgttgagcttcgtgagcgtttcggcgag720ArgIleAsnArgAspValAlaValGluLeuArgGluArgPheGlyGlu225230235240ggtgttcctttgaagcccgtggtcaagccaggttcctggattggtgga768GlyValProLeuLysProValValLysProGlySerTrpIleGlyGly245250255gaccacgacggtaacccttatgtcaccgcggaaacagttgagtattcc816AspHisAspGlyAsnProTyrValThrAlaGluThrValGluTyrSer260265270actcaccgcgctgcggaaaccgtgctcaagtactatgcacgccagctg864ThrHisArgAlaAlaGluThrValLeuLysTyrTyrAlaArgGlnLeu275280285cattccctcgagcatgagctcagcctgtcggaccgcatgaataaggtc912HisSerLeuGluHisGluLeuSerLeuSerAspArgMetAsnLysVal290295300accccgcagctgcttgcgctggcagatgcagggcacaacgacgtgcca960ThrProGlnLeuLeuAlaLeuAlaAspAlaGlyHisAsnAspValPro305310315320agccgcgtggatgagccttatcgacgcgccgtccatggcgttcgcgga1008SerArgValAspGluProTyrArgArgAlaValHisGlyValArgGly325330335cgtatcctcgcgacgacggccgagctgatcggcgaggacgccgttgag1056ArgIleLeuAlaThrThrAlaGluLeuIleGlyGluAspAlaValGlu340345350ggcgtgtggttcaaggtctttactccatacgcatctccggaagaattc1104GlyValTrpPheLysValPheThrProTyrAlaSerProGluGluPhe355360365ttaaacgatgcgttgaccattgatcattctctgcgtgaatccaaggac1152LeuAsnAspAlaLeuThrIleAspHisSerLeuArgGluSerLysAsp370375380gttctcattgccgatgatcgtttgtctgtgctgatttctgccatcgag1200ValLeuIleAlaAspAspArgLeuSerValLeuIleSerAlaIleGlu385390395400agctttggattcaacctttacgcactggatctgcgccaaaactccgaa1248SerPheGlyPheAsnLeuTyrAlaLeuAspLeuArgGlnAsnSerGlu405410415agctacgaggacgtcctcaccgagcttttcgaacgcgcccaagtcacc1296SerTyrGluAspValLeuThrGluLeuPheGluArgAlaGlnValThr420425430gcaaactaccgcgagctgtctgaagcagagaagcttgaggtgctgctg1344AlaAsnTyrArgGluLeuSerGluAlaGluLysLeuGluValLeuLeu435440445aaggaactgcgcagccctcgtccgctgatcccgcacggttcagatgaa1392LysGluLeuArgSerProArgProLeuIleProHisGlySerAspGlu450455460tacagcgaggtcaccgaccgcgagctcggcatcttccgcaccgcgtcg1440TyrSerGluValThrAspArgGluLeuGlyIlePheArgThrAlaSer465470475480gaggctgttaagaaattcgggccacggatggtgcctcactgcatcatc1488GluAlaValLysLysPheGlyProArgMetValProHisCysIleIle485490495tccatggcatcatcggtcaccgatgtgctcgagccgatggtgttgctc1536SerMetAlaSerSerValThrAspValLeuGluProMetValLeuLeu500505510aaggaattcggactcatcgcagccaacggcgacaacccacgcggcacc1584LysGluPheGlyLeuIleAlaAlaAsnGlyAspAsnProArgGlyThr515520525gtcgatgtcatcccactgttcgaaaccatcgaagatctccaggccggc1632ValAspValIleProLeuPheGluThrIleGluAspLeuGlnAlaGly530535540gccggaatcctcgacgaactgtggaaaattgatctctaccgcaactac1680AlaGlyIleLeuAspGluLeuTrpLysIleAspLeuTyrArgAsnTyr545550555560ctcctgcagcgcgacaacgtccaggaagtcatgctcggttactccgat1728LeuLeuGlnArgAspAsnValGlnGluValMetLeuGlyTyrSerAsp565570575tccaacaaggatggcggatatttctccgcaaactgggcgctttacgac1776SerAsnLysAspGlyGlyTyrPheSerAlaAsnTrpAlaLeuTyrAsp580585590gcggaactgcagctcgtcgaactatgccgatcagccggggtcaagctt1824AlaGluLeuGlnLeuValGluLeuCysArgSerAlaGlyValLysLeu595600605cgcctgttccacggccgtggtggcaccgtcggccgcggtggcggacct1872ArgLeuPheHisGlyArgGlyGlyThrValGlyArgGlyGlyGlyPro610615620tcctacgacgcgattcttgcccagcccaggggggctgtccaaggttcc1920SerTyrAspAlaIleLeuAlaGlnProArgGlyAlaValGlnGlySer625630635640gtgcgcatcaccgagcagggcgagatcatctccgctaagtacggcaac1968ValArgIleThrGluGlnGlyGluIleIleSerAlaLysTyrGlyAsn645650655cccgaaaccgcgcgccgaaacctcgaagccctggtctcagccacgctt2016ProGluThrAlaArgArgAsnLeuGluAlaLeuValSerAlaThrLeu660665670gaggcatcgcttctcgacgtctccgaactcaccgatcaccaacgcgcg2064GluAlaSerLeuLeuAspValSerGluLeuThrAspHisGlnArgAla675680685tacgacatcatgagtgagatctctgagctcagcttgaagaagtacgcc2112TyrAspIleMetSerGluIleSerGluLeuSerLeuLysLysTyrAla690695700tccttggtgcacgaggatcaaggcttcatcgattacttcacccagtcc2160SerLeuValHisGluAspGlnGlyPheIleAspTyrPheThrGlnSer705710715720acgccgctgcaggagattggatccctcaacatcggatccaggccttcc2208ThrProLeuGlnGluIleGlySerLeuAsnIleGlySerArgProSer725730735tcacgcaagcagacctcctcggtggaagatttgcgagccatcccatgg2256SerArgLysGlnThrSerSerValGluAspLeuArgAlaIleProTrp740745750gtgctcagctggtcacagtctcgtgtcatgctgccaggctggtttggt2304ValLeuSerTrpSerGlnSerArgValMetLeuProGlyTrpPheGly755760765gtcggaaccgcattagagcagtggattggcgaaggggagcaggccacc2352ValGlyThrAlaLeuGluGlnTrpIleGlyGluGlyGluGlnAlaThr770775780caacgcattgccgagctgcaaacactcaatgagtcctggccatttttc2400GlnArgIleAlaGluLeuGlnThrLeuAsnGluSerTrpProPhePhe785790795800acctcagtgttggataacatggctcaggtgatgtccaaggcagagctg2448ThrSerValLeuAspAsnMetAlaGlnValMetSerLysAlaGluLeu805810815cgtttggcaaagctctacgcagacctgatcccagatacggaagtagcc2496ArgLeuAlaLysLeuTyrAlaAspLeuIleProAspThrGluValAla820825830gagcgagtctattccgtcatccgcgaggagtacttcctgaccaagaag2544GluArgValTyrSerValIleArgGluGluTyrPheLeuThrLysLys835840845atgttctgcgtaatcaccggctctgatgatctgcttgatgacaaccca2592MetPheCysValIleThrGlySerAspAspLeuLeuAspAspAsnPro850855860cttctcgcacgctctgtccagcgccgatacccctacctgcttccactc2640LeuLeuAlaArgSerValGlnArgArgTyrProTyrLeuLeuProLeu865870875880aacgtgatccaggtagagatgatgcgacgctaccgaaaaggcgaccaa2688AsnValIleGlnValGluMetMetArgArgTyrArgLysGlyAspGln885890895agcgagcaagtgtcccgcaacattcagctgaccatgaacggtctttcc2736SerGluGlnValSerArgAsnIleGlnLeuThrMetAsnGlyLeuSer900905910actgcgctgcgcaactccggc2757ThrAlaLeuArgAsnSerGly915<210>140<211>919<212>PRT<213>谷氨酸棒杆菌<400>140MetThrAspPheLeuArgAspAspIleArgPheLeuGlyGlnIleLeu151015GlyGluValIleAlaGluGlnGluGlyGlnGluValTyrGluLeuVal202530GluGlnAlaArgLeuThrSerPheAspIleAlaLysGlyAsnAlaGlu354045MetAspSerLeuValGlnValPheAspGlyIleThrProAlaLysAla505560ThrProIleAlaArgAlaPheSerHisPheAlaLeuLeuAlaAsnLeu65707580AlaGluAspLeuTyrAspGluGluLeuArgGluGlnAlaLeuAspAla859095GlyAspThrProProAspSerThrLeuAspAlaThrTrpLeuLysLeu100105110AsnGluGlyAsnValGlyAlaGluAlaValAlaAspValLeuArgAsn115120125AlaGluValAlaProValLeuThrAlaHisProThrGluThrArgArg130135140ArgThrValPheAspAlaGlnLysTrpIleThrThrHisMetArgGlu145150155160ArgHisAlaLeuGlnSerAlaGluProThrAlaArgThrGlnSerLys165170175LeuAspGluIleGluLysAsnIleArgArgArgIleThrIleLeuTrp180185190GlnThrAlaLeuIleArgValAlaArgProArgIleGluAspGluIle195200205GluValGlyLeuArgTyrTyrLysLeuSerLeuLeuGluGluIlePro210215220ArgIleAsnArgAspValAlaValGluLeuArgGluArgPheGlyGlu225230235240GlyValProLeuLysProValValLysProGlySerTrpIleGlyGly245250255AspHisAspGlyAsnProTyrValThrAlaGluThrValGluTyrSer260265270ThrHisArgAlaAlaGluThrValLeuLysTyrTyrAlaArgGlnLeu275280285HisSerLeuGluHisGluLeuSerLeuSerAspArgMetAsnLysVal290295300ThrProGlnLeuLeuAlaLeuAlaAspAlaGlyHisAsnAspValPro305310315320SerArgValAspGluProTyrArgArgAlaValHisGlyValArgGly325330335ArgIleLeuAlaThrThrAlaGluLeuIleGlyGluAspAlaValGlu340345350GlyValTrpPheLysValPheThrProTyrAlaSerProGluGluPhe355360365LeuAsnAspAlaLeuThrIleAspHisSerLeuArgGluSerLysAsp370375380ValLeuIleAlaAspAspArgLeuSerValLeuIleSerAlaIleGlu385390395400SerPheGlyPheAsnLeuTyrAlaLeuAspLeuArgGlnAsnSerGlu405410415SerTyrGluAspValLeuThrGluLeuPheGluArgAlaGlnValThr420425430AlaAsnTyrArgGluLeuSerGluAlaGluLysLeuGluValLeuLeu435440445LysGluLeuArgSerProArgProLeuIleProHisGlySerAspGlu450455460TyrSerGluValThrAspArgGluLeuGlyIlePheArgThrAlaSer465470475480GluAlaValLysLysPheGlyProArgMetValProHisCysIleIle485490495SerMetAlaSerSerValThrAspValLeuGluProMetValLeuLeu500505510LysGluPheGlyLeuIleAlaAlaAsnGlyAspAsnProArgGlyThr515520525ValAspValIleProLeuPheGluThrIleGluAspLeuGlnAlaGly530535540AlaGlyIleLeuAspGluLeuTrpLysIleAspLeuTyrArgAsnTyr545550555560LeuLeuGlnArgAspAsnValGlnGluValMetLeuGlyTyrSerAsp565570575SerAsnLysAspGlyGlyTyrPheSerAlaAsnTrpAlaLeuTyrAsp580585590AlaGluLeuGlnLeuValGluLeuCysArgSerAlaGlyValLysLeu595600605ArgLeuPheHisGlyArgGlyGlyThrValGlyArgGlyGlyGlyPro610615620SerTyrAspAlaIleLeuAlaGlnProArgGlyAlaValGlnGlySer625630635640ValArgIleThrGluGlnGlyGluIleIleSerAlaLysTyrGlyAsn645650655ProGluThrAlaArgArgAsnLeuGluAlaLeuValSerAlaThrLeu660665670GluAlaSerLeuLeuAspValSerGluLeuThrAspHisGlnArgAla675680685TyrAspIleMetSerGluIleSerGluLeuSerLeuLysLysTyrAla690695700SerLeuValHisGluAspGlnGlyPheIleAspTyrPheThrGlnSer705710715720ThrProLeuGlnGluIleGlySerLeuAsnIleGlySerArgProSer725730735SerArgLysGlnThrSerSerValGluAspLeuArgAlaIleProTrp740745750ValLeuSerTrpSerGlnSerArgValMetLeuProGlyTrpPheGly755760765ValGlyThrAlaLeualuGlnTrpIleGlyGluGlyGluGlnAlaThr770775780GlnArgIleAlaGluLeuGlnThrLeuAsnGluSerTrpProPhePhe785790795800ThrSerValLeuAspAsnMetAlaGlnValMetSerLysAlaGluLeu805810815ArgLeuAlaLysLeuTyrAlaAspLeuIleProAspThrGluValAla820825830GluArgValTyrSerValIleArgGluGluTyrPheLeuThrLysLys835840845MetPheCysValIleThrGlySerAspAspLeuLeuAspAspAsnPro850855860LeuLeuAlaArgSerValGlnArgArgTyrProTyrLeuLeuProLeu865870875880AsnValIleGlnValGluMetMetArgArgTyrArgLysGlyAspGln885890895SerGluGlnValSerArgAsnIleGlnLeuThrMetAsnGlyLeuSer900905910ThrAlaLeuArgAsnSerGly915<210>141<211>927<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(927)<223>RXA00970W<400>141atggcaattgaactgaacgtcggtcgtaaggttaccgtcacggtacct48MetAlaIleGluLeuAsnValGlyArgLysValThrValThrValPro151015ggatcttctgcaaacctcggacctggctttgacactttaggtttggca96GlySerSerAlaAsnLeuGlyProGlyPheAspThrLeuGlyLeuAla202530ctgtcggtatacgacactgtcgaagtggaaattattccatctggcttg144LeuSerValTyrAspThrValaluValaluIleIleProSerGlyLeu354045gaagtggaagtttttggcgaaggccaaggcgaagtccctcttgatggc192GluValGluValPheGlyGluGlyGlnGlyGluValProLeuAspGly505560tcccacctggtggttaaagctattcgtgctggcctgaaggcagctgac240SerHisLeuValValLysAlaIleArgAlaGlyLeuLysAlaAlaAsp65707580gctgaagttcctggattgcgagtggtgtgccacaacaacattccgcag288AlaGluValProGlyLeuArgValValCysHisAsnAsnIleProGln859095tctcgtggtcttggctcctctgctgcagcggcggttgctggtgttgct336SerArgGlyLeuGlySerSerAlaAlaAlaAlaValAlaGlyValAla100105110gcagctaatggtttggcggatttcccgctgactcaagagcagattgtt384AlaAlaAsnGlyLeuAlaAspPheProLeuThrGlnGluGlnIleVal115120125cagttgtcctctgcctttgaaggccacccagataatgctgcggcttct432GlnLeuSerSerAlaPheGluGlyHisProAspAsnAlaAlaAlaSer130135140gtgctgggtggagcagtggtgtcgtggacaaatctgtctatcgacggc480ValLeuGlyGlyAlaValValSerTrpThrAsnLeuSerIleAspGly145150155160aagagccagccacagtatgctgctgtaccacttgaggtgcaggacaat528LysSerGlnProGlnTyrAlaAlaValProLeuGluValGlnAspAsn165170175attcgtgcgactgcgctggttcctaatttccacgcatccaccgaagct576IleArgAlaThrAlaLeuValProAsnPheHisAlaSerThrGluAla180185190gtgcgccgagtccttcccactgaagtcactcacatcgatgcgcgattt624ValArgArgValLeuProThrGluValThrHisIleAspAlaArgPhe195200205aacgtgtcccgcgttgcagtgatgatcgttgcgttgcagcagcgtcct672AsnValSerArgValAlaValMetIleValAlaLeuGlnGlnArgPro210215220gatttgctgtgggagggtactcgtgaccgtctgcaccagccttatcgt720AspLeuLeuTrpGluGlyThrArgAspArgLeuHisGlnProTyrArg225230235240gcagaagtgttgcctattacctctgagtgggtaaaccgcctgcgcaac768AlaGluValLeuProIleThrSerGluTrpValAsnArgLeuArgAsn245250255cgtggctacgcggcatacctttccggtgccggcccaaccgccatggtg816ArgGlyTyrAlaAlaTyrLeuSerGlyAlaGlyProThrAlaMetVal260265270ctgtccactgagccaattccagacaaggttttggaagatgctcgtgag864LeuSerThrGluProIleProAspLysValLeuGluAspAlaArgGlu275280285tctggcattaaggtgcttgagcttgaggttgcgggaccagtcaaggtt912SerGlyIleLysValLeuGluLeuGluValAlaGlyProValLysVal290295300gaagttaaccaacct927GluValAsnGlnPro305<210>142<211>309<212>PRT<213>谷氨酸棒杆菌<400>142MetAlaIleGluLeuAsnValGlyArgLysValThrValThrValPro151015GlySerSerAlaAsnLeuGlyProGlyPheAspThrLeuGlyLeuAla202530LeuSerValTyrAspThrValGluValGluIleIleProSerGlyLeu354045GluValGluValPheGlyGluGlyGlnGlyGluValProLeuAspGly505560SerHisLeuValValLysAlaIleArgAlaGlyLeuLysAlaAlaAsp65707580AlaGluValProGlyLeuArgValValCysHisAsnAsnIleProGln859095SerArgGlyLeuGlySerSerAlaAlaAlaAlaValAlaGlyValAla100105110AlaAlaAsnGlyLeuAlaAspPheProLeuThrGlnGluGlnIleVal115120125GlnLeuSerSerAlaPneGluGlyHisProAspAsnAlaAlaAlaSer130135140ValLeuGlyGlyAlaValValSerTrpThrAsnLeuSerIleAspGly145150155160LysSerGlnProGlnTyrAlaAlaValProLeuGluValGlnAspAsn165170175IleArgAlaThrAlaLeuValProAsnPheHisAlaSerThrGluAla180185190ValArgArgValLeuProThrGluValThrHisIleAspAlaArgPhe195200205AsnValSerArgValAlaValMetIleValAlaLeuGlnGlnArgPro210215220AspLeuLeuTrpGluGlyThrArgAspArgLeuHisGlnProTyrArg225230235240AlaGluValLeuProIleThrSerGluTrpValAsnArgLeuArgAsn245250255ArgGlyTyrAlaAlaTyrLeuSerGlyAlaGlyProThrAlaMetVal260265270LeuSerThrGluProIleProAspLysValLeuGluAspAlaArgGlu275280285SerGlyIleLysValLeuGluLeuGluValAlaGlyProValLysVal290295300GluValAsnGlnPro30权利要求1.具有表1/第7列所指定的每种情况下的功能并具有下列所述氨基酸序列的一种蛋白质，所述氨基酸序列即在从表1/第2列每一情况下所提到的氨基酸序列开始的、对应于表1/第4列中对该氨基酸序列所指定的氢基酸位置中的至少一个位置上具有与表1/第5列同一行中所指定的特定氨基酸不同的生蛋白氨基酸，其前提条件是根据表2的蛋白质除外。2.如权利要求1所述的蛋白质，其中该蛋白质具有表1/第2列每一情况下所提到的氨基酸序列，其中该蛋白质在表1/第4列中对该氨基酸序列所指定的氨基酸位置中的至少一个位置上具有与表1/第5列同一行中所指定的特定氢基酸不同的生蛋白氨基酸。3.如权利要求2所述的蛋白质，其中该蛋白质在表1/第4列中对该氨基酸序列所指定的氨基酸位置中的至少一个位置上具有表1/第6列中同一行所指定的氨基酸。4.一种分离的核酸，其编码如权利要求1至3中任一项所述的蛋白质。5.一种分离的核酸构建体，其包含至少一种如权利要求4所述的核酸。6.如权利要求5所述的核酸构建体，其包含功能性连接形式的启动子和视需要而定的终止子。7.一种通过转化非人母体生物而制备非人经基因修饰的生物的方法，其中所述转化是向该母体生物中导入a)至少一种如权利要求4所述的核酸，或b)至少一种如权利要求5或6所述的核酸构建体，或c)对于如权利要求4所述内源MP核酸而言为外源的并且能够使如权利要求4所述的该内源核酸在该生物中表达的启动子。8.如权利要求7所述的方法，其中将如权利要求7中实施方案a)所述的核酸或者如权利要求7中实施方案b)所述的核酸构建体以作为复制型质粒或染色体整合形式导入。9.如权利要求7所述的方法，其中将如权利要求7中实施方案c)所述的启动子与如权利要求4所述的内源核酸在该生物中进行功能性连接。10.一种经基因修饰的微生物，其通过如权利要求7至9中任一项所述的方法得到。11.一种经基因修饰的非人生物，其用下列所述进行转化a)至少一种如权利要求4所述的核酸，或b)至少一种如权利要求5或6所述的核酸构建体，或c)对于如权利要求4所述内源MP核酸而言为外源的并且能够使如权利要求4所述的该内源核酸在该生物中表达的启动子。12.如权利要求10或11所述的经基因修饰的生物，其中所使用的母体生物为已经能够生产精细化学品的生物。13.如权利要求10至12中任一项所述的经基因修饰的生物，其中与母体生物相比该核酸的表达导致该生物中精细化学品生产的调节。14.一种制备精细化学品的方法，其通过培养如权利要求10至13中任一项所述的经基因修饰的生物制备。15.一种制备精细化学品的方法，其通过以下制备A)用下列所述转化非人母体生物a)至少一种如权利要求4所述的核酸，或b)至少一种如权利要求5或6所述的核酸构建体，或c)对于如权利要求4所述内源MP核酸而言为外源的并且能够使如权利要求4所述的该内源核酸在该生物中表达的启动子，和B)培养根据A)项制备的经基因修饰的生物。16.如权利要求14或15所述的方法，其中在培养后将至少一种精细化学品从经基因修饰生物和/或培养基中分离。17.如权利要求14至16中任一项所述的方法，其中经基因修饰的生物为微生物。18.如权利要求17所述的方法，其中微生物选自棒杆菌属(Corynebacterium)细菌或短杆菌属(Brevibacterium)细菌。19.如权利要求14至18中任一项所述的方法，其中精细化学品为氨基酸。20.如权利要求19所述的方法，其中氨基酸选自L-赖氨酸、L-苏氨酸和L-甲硫氨酸。全文摘要本发明涉及精细化学品代谢途径中的经突变核酸和蛋白质、制备经基因修饰生产者生物的方法、通过培养所述经基因修饰生物制备精细化学品的方法以及所述经基因修饰生物本身。文档编号C12N15/31GK1898259SQ200480037800公开日2007年1月17日申请日期2004年12月16日优先权日2003年12月18日发明者C·克洛普罗格,O·策尔德尔,B·克勒格尔,H·施罗德,S·哈夫纳,U·卢菲尔,C·I·格拉夫,G·哈伯豪尔申请人:巴斯福股份公司