基因和蛋白质及它们的用途的制作方法

专利名称：基因和蛋白质及它们的用途的制作方法
技术领域：
本发明涉及细菌性基因和蛋白质以及它们的用途。本发明尤其涉及它们在治疗中的用途，用于免疫和筛选药物。
背景技术：
脑膜炎奈瑟球菌(Neisseria meningitidis)是脓毒性休克和细菌性脑膜炎中含有的革兰氏阴性(Gram-negative)细菌性病原体。该细菌是在发展国家中细菌性脑膜炎的主要病因，并在非洲和中国引起大规模的流行。在英国，脑膜炎奈瑟球菌是除公路交通事故之外导致儿童死亡的主要原因。该细菌天然地存在于人鼻咽部，然后进入血流，并由此引起严重败血病和脑膜炎。尽管目前抗微生物剂对从体内去除该细菌非常有效，脑膜炎球菌性败血症引起的死亡率仍然非常大。希望提供治疗或预防脑膜炎奈瑟球菌引起的疾病的方法，即免疫方法。
发明概述本发明基于在脑膜炎奈瑟球菌中发现的基因，其产物可位于生物体的外表面，因此可能用作免疫治疗的靶标。
本发明的一方面是具有任何一种权利要求1确定的核苷酸序列的基因编码的肽，或其同源性或功能性片段。该片段(例如分离的)适于治疗或诊断应用。
本发明的另一方面是编码上述肽的多核苷酸，其也可用于治疗和诊断。
本发明的再一方面是可用于筛选潜在抗微生物药物的肽或多核苷酸。
本发明的又一方面是此处确定的任何产物的用途，用于奈瑟菌属(Neisseria)或革兰氏阴性菌感染相关的疾病的治疗或预防。
发明详述本发明是基于编码位于奈瑟菌属的细胞表面的肽的基因的发现，因此其对于治疗感染的治疗试剂的制备非常有用。应当理解治疗也包括预防性治疗，例如接种。另外，尽管本发明的产物主要用于人类病人的感染，但认为兽医上的应用也在本发明的范围之内。
参考脑膜炎奈瑟球菌描述本发明。但是所有的奈瑟球菌菌株以及许多其它革兰氏阴性细菌菌株也可能包括与此处确定的蛋白质或肽具有同一或相似的氨基酸序列的蛋白质或肽。可能含有所述肽的生物体包括但不限于沙门氏菌属(Salmonella)、肠杆菌属(Enterobacter)、克雷白菌属(Klebsiella)、志贺菌属(Shigella)和耶尔森菌属(Yersinia)。
优选地，可能在本发明的各方面有用的肽与此处所确定的肽具有40％以上的相似性。更优选地，所述肽具有60％以上的序列相似性。最优选地，所述肽具有80％以上的序列相似性，例如95％的相似性。关于此处确定的多核苷酸序列，在本发明的各方面有用的有关多核苷酸与此处确定的序列具有40％以上的同一性。更优选地，所述多核苷酸序列具有60％以上的序列同一性。最优选地，所述多核苷酸序列具有80％以上的序列同一性，例如95％的同一性。
术语“相似性”和“同一性”本领域已知。使用术语“同一性”指基于在被比较的序列的相应的相同位置之间的同一性匹配的序列比对。术语“相似性”指氨基酸序列之间的比对，不仅考虑相应位置的相同的氨基酸，并考虑相应位置功能相似的氨基酸。因此，多肽序列之间的相似性指序列上相似外，还指功能上相似。
可以利用已知的方法计算基因序列之间同一性水平和氨基酸序列之间同一性或相似性水平。关于本发明，用于确定同一性和相似性的公开可用的基于计算机的方法包括BLASTP、BALSTN和FASTA(Atschul等人，J.Molec.Biol.，1990；215403-410)，BLASTX程序可获自NCBI，Gap程序获自Genetics Computer Group，Madison WI。利用Gap程序获得此处提供的相似性和同一性水平，利用Gap罚分为12和Gap长度罚分为4来确定氨基酸序列比对，用Gap罚分为50和Gap长度罚分为3来确定多核苷酸序列比对。
对本发明的基因性质鉴定以后，就可能利用该基因序列在其它微生物中检索相关的基因。这可能通过检索现有的数据库(例如EMBL或GenBank)来进行。
此处所述的技术为本领域熟知。但是，特别涉及Sambrook等人，Molecular Cloning，A Laboratory Manual(1989)以及Ausubel等人，current Protocols in Molecular Biology(1995)，JohnWiley&Sons，Inc.
可以利用本领域已知的方法纯化和分离本发明的肽或蛋白质。具体地，确定了基因序列以后，就可能利用重组技术在合适的宿主中表达该基因。可以确定活性片段和有关的分子，并可能用于治疗。例如，肽或它们的活性片段可用作疫苗中的抗原性决定簇，以激发免疫应答。它们也可用于抗体制备，用于被动免疫或诊断应用。合适的抗体包括单克隆抗体或其片段，包括单链Fv片段。人源化的抗体也在本发明的范围内。抗体的制备方法对本领域技术人员是显而易见的。
肽的活性片段是指保持肽的生物学功能的片段。例如，用于激发免疫应答时，片段应具有足够的大小，使该片段产生的抗体能区别该肽和细菌微生物上的其它肽。通常，片段大小为至少30个核苷酸(10个氨基酸)，优选60个核苷酸(20个氨基酸)，最优选大于90个核苷酸(30个氨基酸)。
应当理解，除了其它微生物的相关分子外，本发明包括对此处确定的肽和多核苷酸所作的不明显改变生物学功能的修饰。遗传密码子的简并性可以由此处所述的多核苷酸产生具有小的碱基改变、但编码相同的肽的多核苷酸，这对本领域技术人员是显而易见的。本发明也包括互补的多核苷酸。也包括氨基酸水平的保守性置换，即可以置换不同的酸性或碱性氨基酸而基本不丧失功能。
本领域技术人员已知基于确定的肽制备疫苗。可以用合适的载体或佐剂配制疫苗组合物(例如明矾，必须或希望的)，以提供针对感染的有效的免疫。疫苗制剂的制备对技术人员来说是显而易见的。
比较普遍地，并且本领域技术人员熟知，为了治疗应用，可以选择合适量的本发明活性组分以及合适的载体或赋型剂和给药途径。可以根据已知的标准(例如欲治疗的疾病的性质/严重程度、受试者的类型和/或健康等)选择或决定这些因素。
在单独的实施方案中，本发明的产物可能用于确定潜在抗微生物药物或检测毒力的筛选分析中。本领域技术人员知道常规的筛选分析，并可以利用本发明的产物进行适当方式的改动。例如，本发明的产物可能用作潜在药物的靶标，药物失活或结合靶标的能力表明其潜在的抗微生物活性。
本发明的基因也可能与微生物的毒力有关，因此缺失或失活该基因可能有效产生减毒的(无毒的)微生物。
可制备减毒的微生物具有破坏此处确定的任何基因的表达的突变。技术人员应知道破坏特定基因表达的方法。可能使用的技术包括插入失活或基因缺失技术。本发明减毒的微生物也可能包括其它在基因上的另外的突变，例如，在此处鉴定的另一种基因上，或在微生物生长所需的单独的基因上，例如，aro突变，或对于沙门菌属来说，在位于WO-A-96/17951确定的SP12区的基因上。
减毒的微生物也可能用作载体系统，用于传递异源抗原、治疗性蛋白质或核酸(DNA或RNA)。在该实施方案中，用减毒的微生物来传递异源抗原、蛋白质或核酸到体内的特定位点。可以通过常规技术(包括使用重组构建体，例如包含表达异源抗原或治疗性蛋白质的多核苷酸以及合适的启动子序列的载体)将异源抗原、肽或核酸引入到减毒的微生物中。另外，可将编码异源抗原或蛋白质的基因引入到生物体的基因组中，用内源启动子控制表达。
本发明的各种产物也可能用于兽医上的应用。
本发明确定的肽如下肽的确定利用质粒载体pFW-phoA1、pFW-phoA2和pFW-phoA3(Podbielski，A.等人，Gene 1996；177137-147)制备脑膜炎奈瑟球菌(菌株C311+)染色体DNA部分基因文库。这些载体具有组成性壮观霉素腺苷酰转移酶抗生素抗性标志，其产生高水平的壮观霉素抗性，因此容易选择。另外，这些载体含有截短的(缺乏前导序列的)大肠杆菌(Escherichia coli)碱性磷酸酶pho A基因。比较上游读框内的BamHI限制性酶切位点时，这三个载体只是缺乏前导序列的pho A基因存在的阅读框架不同。因为该截短的大肠杆菌pho A基因缺乏合适的前导序列使该酶输出过细菌膜，因此这些质粒在大肠杆菌pho A突变体(例如菌株DH5α)中增殖时没有细胞外碱性磷酸酶活性。显色碱性磷酸酶底物XP(5-溴-4-氯-3-吲哚-磷酸)不进入完整的细菌细胞，因此只能检测到输出的或表面结合的碱性磷酸酶活性。当存在输出的或表面结合的碱性磷酸酶活性时，显色底物XP被切割产生蓝色色素，通过其蓝色可以确定相应的细菌克隆。
质粒DNA用BamHI完全消化，用虾碱性磷酸酶去磷酸化。奈瑟球菌属基因组DNA用Sau3AI部分消化，使大部分的片段在用溴化乙锭染色的1％的琼脂糖凝胶上观察时呈现0.5-1.0kb大小。这些Sau3AI片段连接至制备好的pFW-phoA载体中。选择大肠杆菌菌株DH5α作为克隆宿主，因为其缺乏功能性phoA基因。在含有100μg/ml的壮观霉素和40μg/ml的XP显色底物的Luria琼脂上选择重组质粒。通过克隆的蓝色确定带有含有脑膜炎奈瑟球菌插入DNA的质粒的大肠杆菌转化子，脑膜炎奈瑟球菌插DNA补偿了缺乏前导序列的phoA基因的输出信号序列。
测序补偿了缺乏前导序列的phoA基因的输出信号序列的脑膜炎奈瑟球菌插入DNA，得到的序列检索GenBank数据库的已知蛋白质。结果示于表1。
表1
候选蛋白质疫苗的保护性性质筛选中确定的基因被估定为潜在的蛋白质疫苗候选者，其基于克隆的、表达的蛋白质在兔中产生免疫应答的能力，产生的抗体具有刺激补体介导的脑膜炎奈瑟球菌的溶菌作用的能力。通过活菌攻击用重组蛋白质免疫的小鼠来确定保护应答。
总之，PCR扩增、克隆候选者基因，编码的蛋白质表达并纯化。纯化的蛋白质用于产生用于酶联免疫吸附测定(ELISA)的抗体。也PCR扩增、克隆、表达和纯化PorA基因。PorA的单克隆抗体在幼兔感染模型中显示被动保护动物(Saukkonen等人，Microb.Pathog.，1987；3(4)261-267)。因此，该蛋白质用作一些试验中的阳性对照。已经表明PorA不能保护动物抵抗不同菌株的脑膜炎奈瑟球菌的攻击(Poolman，Infect.Dis.，1995；413)，因此，任何能针对不同种类的脑膜炎奈瑟球菌菌株产生保护性免疫应答的候选者都比PorA有优势。
PCR扩增DNA利用来自菌株MC58的基因组DNA作为DNA模板，PCR扩增候选者基因(McGuinness等人，Lancet，1991；337514)。引物列于表2。F代表正向引物，R代表反向引物。引物对PRAF和PRAR用来扩增PorA基因DNA。
表2
克隆疫苗候选者将从候选者PCR扩增的DNA直接克隆到InVitrogen pCRT7/CT-TOPO载体中。该载体提供了T7启动子、核糖体结合位点和C-末端6×His标签融合体以促进表达和利用金属亲和层析的重组蛋白质的纯化。
为了克隆，将连接反应物转化进T0P10F’细胞(InVitrogen)。筛选来自转化子DNA克隆的DNA样品来检查插入DNA的方向。测序具有正确方向的插入DNA的候选者克隆以证实构建体5’区域的整合。表达和纯化克隆的候选者转化到HMS174(DE3)pLysS感受态细胞(InVitrogen)后检测候选者基因的表达。用IPTG诱导候选者克隆的表达，并在诱导4小时后利用抗His抗体通过SDS PAGE和Western印迹分析表达。通过Talon树酯(利用在蛋白质的羧基末端克隆的6×His-标签的金属亲和层析柱(Clontech))，利用咪唑(imidozole)缓冲液从树酯上梯度洗脱大蛋白质(10-100mM)，进行候选者蛋白质的纯化。
抗体纯化抗体纯化前，预筛选在ELISA分析中对全细胞脑膜炎奈瑟球菌低反应原性的动物血清。利用100μg的蛋白质与弗氏佐剂进行起始接种，并与弗氏不完全佐剂间隔28天进行随后三次加强免疫，在兔子中产生针对每一克隆的和纯化的候选者的抗体。每次加强免疫后收集血清产生血清样本。
针对全部热杀死的脑膜炎奈瑟球菌的ELISA进行针对热杀死的脑膜炎奈瑟球菌的ELISA以证实针对纯化的蛋白质的抗体识别脑膜炎奈瑟球菌细胞。这些分析在菌株MC58以及下列菌株上进行脑膜炎奈瑟球菌(B)型1000脑膜炎奈瑟球菌(B)型SW2 107脑膜炎奈瑟球菌(B)型NGH38
脑膜炎奈瑟球菌(B)型NGE28脑膜炎奈瑟球菌(B)型2996。这些菌株都是盛行性致病菌株，并且涵盖了基于MLST(多基因座序列分型)产生的进化系统树的该物种的遗传多样性。
热杀死的脑膜炎奈瑟球菌的制备脑膜炎奈瑟球菌在巧克力的马血Columbia琼脂(Oxoid)上37℃、5％CO2生长14小时。从琼脂平板上刮下细胞，重悬细胞于50ml试管中的20mlPBS中。该细胞悬液在56℃加热30分钟以杀死细菌。
50μl热杀死的脑膜炎奈瑟球菌样品铺到巧克力的马血Columbia琼脂(Oxoid)上37℃、5％CO2温育18小时。这证实所有的脑膜炎奈瑟球菌都已经被杀死。然后用PBS洗涤热杀死的细胞。用PBS将悬液的OD620调整到0.1OD单位。
热杀死的脑膜炎奈瑟球菌的ELISA用热杀死的全细胞脑膜炎奈瑟球菌进行ELISA分析。用热杀死的细胞过夜包被ELISA平板(96孔板的每一孔中加入50μl杀死的细菌PBS，4℃温育)。然后进行标准的EILSA程序，全部温育都在37℃、1小时。使用PBS/3％BSA封闭溶液、PBS/Tween 0.1％洗涤溶液、抗兔AP缀合第二抗体(Sigma)和Sigma Fast P硝基苯磷酸检测剂(Sigma)。利用合适的微量滴定板读数仪读取405nm处的数据。利用首次加强接种后7天(首次接种后的35天)获得的血清产生该数据。
ELISA数据结果表明针对每一候选蛋白质产生的抗血清激发针对脑膜炎奈瑟球菌不同菌株的强烈应答。
体内筛选为评价疫苗候选者的保护效能，用重组蛋白质免疫成年小鼠，通过活细菌攻击确定保护性应答。
对于每一种疫苗候选者，用25μg抗原间隔3周两次单独接种15只6周龄balb/C小鼠(皮下)。免疫程序结束后一周，用同源菌株MC58攻击该实验组。腹膜内接种500μl含细菌的Brain HeartInfusion/0.5％铁葡聚糖介质，剂量为1×106cfu。以前的结果已经表明在这些动物中起始菌血性(bacteraemic)疾病需要铁。该模型以前曾经用来证明接种疫苗的保护性效能(Lissolo等，Infect.Immun.，1995；63884-890)。
对照组包括只用佐剂接种的动物(阴性对照)、用佐剂和纯化的PorA联合接种的动物(阳性对照)或减毒的同源菌株接种的动物。攻击后监测存活。
用候选者pho2-5接种的动物与未接种的对照相比显示80％(12/15)的存活，未接种的对照13％(2/15)存活。候选者pho2-5显示的保护水平与pro蛋白质相当(13/15)。
用候选者pho2-10、pho1-94、pho2-66接种的动物与未接种的对照相比分别显示40％(6/15)、47％(7/15)或27％(4/15)的存活，未接种的对照13％(2/15)存活。
序列表<110>Microscience Limited<120>基因和蛋白质及其用途<130>REP06516WO<140>(未知)<141>2001-08-21<150>0020952.8<151>2000-08-24<160>48<170>PatentIn Ver.2.1<210>1<211>939<212>DNA<213>脑膜炎奈瑟球菌(Neisseria meningitidis)<220>
<221>CDS<222>(1)..(939)<400>1atg tcc atc cat act ctg aaa cgc ctg ccc tca tcg ctg ctg ctc ggt48Met Ser Ile His Thr Leu Lys Arg Leu Pro Ser Ser Leu Leu Leu Gly1 5 10 15ctc tgc ctt tcc ctg ccg tca gcc cac ctt ttt gcc gac aac gac att96Leu Cys Leu Ser Leu Pro Ser Ala His Leu Phe Ala Asp Asn Asp Ile20 25 30tta ggg caa ttt tta gaa cag aac atg ctt acc tcc tcc gat ccg ata144Leu Gly Gln Phe Leu Glu Gln Asn Met Leu Thr Ser Ser Asp Pro Ile35 40 45gaa ata ttc gcc gaa agc acg ata cac ccc acc aac acc caa gcc att192Glu Ile Phe Ala Glu Ser Thr Ile His Pro Thr Asn Thr Gln Ala Ile50 55 60aca ggc ggt ctg att ctc tcc tca cag tct gcc ctg gtc gtc aac aac240Thr Gly Gly Leu Ile Leu Ser Ser Gln Ser Ala Leu Val Val Asn Asn65 70 75 80
aaa acc gga cag ata ctg tat cag aaa aac gcc gac agg att atg ccc288Lys Thr Gly Gln Ile Leu Tyr Gln Lys Asn Ala Asp Arg Ile Met Pro85 90 95atc gcc tcc att tcc aaa ctg atg agc gcg atg gtc gtt ttg gat gca336Ile Ala Ser Ile Ser Lys Leu Met Ser Ala Met Val Val Leu Asp Ala100 105 110aac ttg gac atg aac gaa acc gtt acc att acg ccc gac gaa atc gac384Asn Leu Asp Met Asn Glu Thr Val Thr Ile Thr Pro Asp Glu Ile Asp115 120 125cgc atc aaa ggg acc ggc agc cgt ctt gcc ata ggt acg gca ctt aca432Arg Ile Lys Gly Thr Gly Ser Arg Leu Ala Ile Gly Thr Ala Leu Thr130 135 140cgc aaa aaa ctg ctg cac ctg agc ctg atg agc agc gaa aac cgc gcc480Arg Lys Lys Leu Leu His Leu Ser Leu Met Ser Ser Glu Asn Arg Ala145 150 155 160acc cat gca ttg ggc aga acc tac ccc ggc ggc atg ggc gca ttt gtc528Thr His Ala Leu Gly Arg Thr Tyr Pro Gly Gly Met Gly Ala Phe Val165 170 175gcc gcc atg aac cgc aaa gcc caa agc ctc ggt atg tac ggc agc cgc576Ala Ala Met Asn Arg Lys Ala Gln Ser Leu Gly Met Tyr Gly Ser Arg180 185 190ttt tac gaa ccg acc gga ctc aac ttc caa aac gtt tct acc gcc aaa624Phe Tyr Glu Pro Thr Gly Leu Asn Phe Gln Asn Val Ser Thr Ala Lys195 200 205gac ctg agc ctt atg gtc aac gcc gcc gcc caa tat ccg caa atc cgc672Asp Leu Ser Leu Met Val Asn Ala Ala Ala Gln Tyr Pro Gln Ile Arg210 215 220acc aac tcg act tcc aac tac gcc tcg gta cag acc aaa aac ggg cag720Thr Asn Ser Thr Ser Asn Tyr Ala Ser Val Gln Thr Lys Asn Gly Gln225 230 235 240cag aac tac aaa aac tcc aat gcc ctg gtc aga gaa ggc atg tgg aac768Gln Asn Tyr Lys Asn Ser Asn Ala Leu Val Arg Glu Gly Met Trp Asn245 250 255atc gaa ttg cag aaa acc ggc tac ata cgc gaa gca ggc agg tct atg816Ile Glu Leu Gln Lys Thr Gly Tyr Ile Arg Glu Ala Gly Arg Ser Met260 265 270
gtt gtc aaa gcc aac att caa aac caa ccc gtt acc atc gta ttg ctg864Val Val Lys Ala Asn Ile Gln Asn Gln Pro Val Thr Ile Val Leu Leu275 280 285aac tcg ccc aca tcc gcc aca cgc gtc aac gac gcc cgc aaa atc gaa912Asn Ser Pro Thr Ser Ala Thr Arg Val Asn Asp Ala Arg Lys Ile Glu290 295 300tcg tgg atg ctg cag caa cgc tcc tga939Ser Trp Met Leu Gln Gln Arg Ser305 310<210>2<211>312<212>PRT<213>脑膜炎奈瑟球菌<400>2Met Ser Ile His Thr Leu Lys Arg Leu Pro Ser Ser Leu Leu Leu Gly1 5 10 15Leu Cys Leu Ser Leu Pro Ser Ala His Leu Phe Ala Asp Asn Asp Ile20 25 30Leu Gly Gln Phe Leu Glu Gln Asn Met Leu Thr Ser Ser Asp Pro Ile35 40 45Glu Ile Phe Ala Glu Ser Thr Ile His Pro Thr Asn Thr Gln Ala Ile50 55 60Thr Gly Gly Leu Ile Leu Ser Ser Gln Ser Ala Leu Val Val Asn Asn65 70 75 80Lys Thr Gly Gln Ile Leu Tyr Gln Lys Asn Ala Asp Arg Ile Met Pro85 90 95Ile Ala Ser Ile Ser Lys Leu Met Ser Ala Met Val Val Leu Asp Ala100 105 110Asn Leu Asp Met Asn Glu Thr Val Thr Ile Thr Pro Asp Glu Ile Asp115 120 125Arg Ile Lys Gly Thr Gly Ser Arg Leu Ala Ile Gly Thr Ala Leu Thr130 135 140Arg Lys Lys Leu Leu His Leu Ser Leu Met Ser Ser Glu Asn Arg Ala145 150 155 160
Thr His Ala Leu Gly Arg Thr Tyr Pro Gly Gly Met Gly Ala Phe Val165 170 175Ala Ala Met Asn Arg Lys Ala Gln Ser Leu Gly Met Tyr Gly Ser Arg180 185 190Phe Tyr Glu Pro Thr Gly Leu Asn Phe Gln Asn Val Ser Thr Ala Lys195 200 205Asp Leu Ser Leu Met Val Asn Ala Ala Ala Gln Tyr Pro Gln Ile Arg210 215 220Thr Asn Ser Thr Ser Asn Tyr Ala Ser Val Gln Thr Lys Asn Gly Gln225 230 235 240Gln Asn Tyr Lys Asn Ser Asn Ala Leu Val Arg Glu Gly Met Trp Asn245 250 255Ile Glu Leu Gln Lys Thr Gly Tyr Ile Arg Glu Ala Gly Arg Ser Met260 265 270Val Val Lys Ala Asn Ile Gln Asn Gln Pro Val Thr Ile Val Leu Leu275 280 285Asn Ser Pro Thr Ser Ala Thr Arg Val Asn Asp Ala Arg Lys Ile Glu290 295 300Ser Trp Met Leu Gln Gln Arg Ser305 310<210>3<211>1239<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(1239)<400>3atg gct ttt tat gct ttt aag gcg atg cgt gcg gcc gcg ttg gct gcc48Met Ala Phe Tyr Ala Phe Lys Ala Met Arg Ala Ala Ala Leu Ala Ala1 5 10 15gcc gtt gca ttg gta ctg tcg tct tgc ggt aaa ggc gga gac gcg gcg96
Ala Val Ala Leu Val Leu Ser Ser Cys Gly Lys Gly Gly Asp Ala Ala20 25 30cag ggc ggg cag cct gct ggt cgg gaa gcc cct gcg ccc gtc gtc ggt144Gln Gly Gly Gln Pro Ala Gly Arg Glu Ala Pro Ala Pro Val Val Gly35 40 45gtc gta acc gtc cat ccg caa acc gtc gca ttg acc gtc gag ttg ccg192Val Val Thr Val His Pro Gln Thr Val Ala Leu Thr Val Glu Leu Pro50 55 60ggg cgt ttg gaa tcg ctg cgt acc gcc gat gtc cgc gcc caa gtc ggc240Gly Arg Leu Glu Ser Leu Arg Thr Ala Asp Val Arg Ala Gln Val Gly65 70 75 80ggc atc atc caa aaa cgc ctg ttc caa gaa ggc agt tat gtc cgt gcc288Gly Ile Ile Gln Lys Arg Leu Phe Gln Glu Gly Ser Tyr Val Arg Ala85 90 95gga cag ccg ctg tat cag atc gac agt tcc act tat gaa gca ggt ctg336Gly Gln Pro Leu Tyr Gln Ile Asp Ser Ser Thr Tyr Glu Ala Gly Leu100 105 110gaa agc gcg cgc gcg caa ctg gca acg gct cag gca acg ctt gcc aaa384Glu Ser Ala Arg Ala Gln Leu Ala Thr Ala Gln Ala Thr Leu Ala Lys115 120 125gcg gat gcg gat ttg gcg cga tac aag cct ttg gtt gcc gcc gaa gcc432Ala Asp Ala Asp Leu Ala Arg Tyr Lys Pro Leu Val Ala Ala Glu Ala130 135 140gtc agc cgg cag gaa tac gat gct gcg gta acg gcg aaa cgt tct gcc480Val Ser Arg Gln Glu Tyr Asp Ala Ala Val Thr Ala Lys Arg Ser Ala145 150 155 160gag gca ggc gtt aaa gcg gcg cag gcg gca atc aaa tcc gcc ggc atc528Glu Ala Gly Val Lys Ala Ala Gln Ala Ala Ile Lys Ser Ala Gly Ile165 170 175agc ctg aac cgt tcg cgc att acc gcg ccg att tcc ggc ttt atc ggt576Ser Leu Asn Arg Ser Arg Ile Thr Ala Pro Ile Ser Gly Phe Ile Gly180 185 190cag tcc aaa gtt tcc gaa ggt acg ttg ctg aac gct ggc gat gcg acc624Gln Ser Lys Val Ser Glu Gly Thr Leu Leu Asn Ala Gly Asp Ala Thr195 200 205gta ctg gcg acc atc cgc caa acc aat ccg atg tat gtg aac gtt acc672
Val Leu Ala Thr Ile Arg Gln Thr Asn Pro Met Tyr Val Asn Val Thr210 215 220cag tct gca tcc gaa gtg atg aaa ttg cgc cgt cag ata gcc gaa ggc720Gln Ser Ala Ser Glu Val Met Lys Leu Arg Arg Gln Ile Ala Glu Gly225 230 235 240aaa ctg ctg gcg gcg gat ggt gtg att gcg gtc ggc atc aaa ttt gac768Lys Leu Leu Ala Ala Asp Gly Val Ile Ala Val Gly Ile Lys Phe Asp245 250 255gac ggc aca gtt tac cct gaa aaa ggc cgc ctg ctg ttt gcc gat ccg816Asp Gly Thr Val Tyr Pro Glu Lys Gly Arg Leu Leu Phe Ala Asp Pro260 265 270gcc gtc aac gaa tcg acc ggt cag att acc ctg cgc gcc gcc gta ccg864Ala Val Asn Glu Ser Thr Gly Gln Ile Thr Leu Arg Ala Ala Val Pro275 280 285aac gat cag aat atc ttg atg ccc ggt ctg tat gtg cgc gtg ctg atg912Asn Asp Gln Asn Ile Leu Met Pro Gly Leu Tyr Val Arg Val Leu Met290 295 300gac caa gtg gcg gtg gat aac gca ttt gtt gtg ccg cag cag gcg gta960Asp Gln Val Ala Val Asp Asn Ala Phe Val Val Pro Gln Gln Ala Val305 310 315 320acg cgc ggt gcg aaa gat acc gtg atg att gtg aat gcc caa ggc ggt1008Thr Arg Gly Ala Lys Asp Thr Val Met Ile Val Asn Ala Gln Gly Gly325 330 335atg gaa ccc cgc gag gta acg gtt gcg caa cag cag ggt acg aat tgg1056Met Glu Pro Arg Glu Val Thr Val Ala Gln Gln Gln Gly Thr Asn Trp340 345 350att gtt acg tcg ggt ctg aag gac ggg gac aag gtg gtt gtg gaa ggc1104Ile Val Thr Ser Gly Leu Lys Asp Gly Asp Lys Val Val Val Glu Gly355 360 365atc agt atc gcc ggt ata acg ggt gcg aaa aag gta acg ccc aaa gaa1152Ile Ser Ile Ala Gly Ile Thr Gly Ala Lys Lys Val Thr Pro Lys Glu370 375 380tgg gcg tcg tct gaa aac caa gcc gcc gcg cct caa tcc ggc gtt cag1200Trp Ala Ser Ser Glu Asn Gln Ala Ala Ala Pro Gln Ser Gly Val Gln385 390 395 400acg gca tct gaa gcc aaa cct gct tct gaa gcg aaa taa1239
Thr Ala Ser Glu Ala Lys Pro Ala Ser Glu Ala Lys405 410<210>4<211>412<212>PRT<213>脑膜炎奈瑟球菌<400>4Met Ala Phe Tyr Ala Phe Lys Ala Met Arg Ala Ala Ala Leu Ala Ala1 5 10 15Ala Val Ala Leu Val Leu Ser Ser Cys Gly Lys Gly Gly Asp Ala Ala20 25 30Gln Gly Gly Gln Pro Ala Gly Arg Glu Ala Pro Ala Pro Val Val Gly35 40 45Val Val Thr Val His Pro Gln Thr Val Ala Leu Thr Val Glu Leu Pro50 55 60Gly Arg Leu Glu Ser Leu Arg Thr Ala Asp Val Arg Ala Gln Val Gly65 70 75 80Gly Ile Ile Gln Lys Arg Leu Phe Gln Glu Gly Ser Tyr Val Arg Ala85 90 95Gly Gln Pro Leu Tyr Gln Ile Asp Ser Ser Thr Tyr Glu Ala Gly Leu100 105 110Glu Ser Ala Arg Ala Gln Leu Ala Thr Ala Gln Ala Thr Leu Ala Lys115 120 125Ala Asp Ala Asp Leu Ala Arg Tyr Lys Pro Leu Val Ala Ala Glu Ala130 135 140Val Ser Arg Gln Glu Tyr Asp Ala Ala Val Thr Ala Lys Arg Ser Ala145 150 155 160Glu Ala Gly Val Lys Ala Ala Gln Ala Ala Ile Lys Ser Ala Gly Ile165 170 175Ser Leu Asn Arg Ser Arg Ile Thr Ala Pro Ile Ser Gly Phe Ile Gly180 185 190Gln Ser Lys Val Ser Glu Gly Thr Leu Leu Asn Ala Gly Asp Ala Thr195 200 205
Val Leu Ala Thr Ile Arg Gln Thr Asn Pro Met Tyr Val Asn Val Thr210 215 220Gln Ser Ala Ser Glu Val Met Lys Leu Arg Arg Gln Ile Ala Glu Gly225 230 235 240Lys Leu Leu Ala Ala Asp Gly Val Ile Ala Val Gly Ile Lys Phe Asp245 250 255Asp Gly Thr Val Tyr Pro Glu Lys Gly Arg Leu Leu Phe Ala Asp Pro260 265 270Ala Val Asn Glu Ser Thr Gly Gln Ile Thr Leu Arg Ala Ala Val Pro275 280 285Asn Asp Gln Asn Ile Leu Met Pro Gly Leu Tyr Val Arg Val Leu Met290 295 300Asp Gln Val Ala Val Asp Asn Ala Phe Val Val Pro Gln Gln Ala Val305 310 315 320Thr Arg Gly Ala Lys Asp Thr Val Met Ile Val Asn Ala Gln Gly Gly325 330 335Met Glu Pro Arg Glu Val Thr Val Ala Gln Gln Gln Gly Thr Asn Trp340 345 350Ile Val Thr Ser Gly Leu Lys Asp Gly Asp Lys Val Val Val Glu Gly355 360 365Ile Ser Ile Ala Gly Ile Thr Gly Ala Lys Lys Val Thr Pro Lys Glu370 375 380Trp Ala Ser Ser Glu Asn Gln Ala Ala Ala Pro Gln Ser Gly Val Gln385 390 395 400Thr Ala Ser Glu Ala Lys Pro Ala Ser Glu Ala Lys405 410<210>5<211>936<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(936)<400>5atg gaa ctc ttt aaa atc aaa cgc gat att ccg ttt atg agc tac ggc48Met Glu Leu Phe Lys Ile Lys Arg Asp Ile Pro Phe Met Ser Tyr Gly1 5 10 15aaa ctg acg acc ttc att tcg ttg gtt acg ttt atc gct gcc gtg ttc96Lys Leu Thr Thr Phe Ile Ser Leu Val Thr Phe Ile Ala Ala Val Phe20 25 30ttt ttg gtt acc aga ggt ctg aat ttc tct gtc gaa ttt acc ggc ggt144Phe Leu Val Thr Arg Gly Leu Asn Phe Ser Val Glu Phe Thr Gly Gly35 40 45acg gta atg gaa gtc caa tat cag cag ggt gcg gat gtc aat aag atg192Thr Val Met Glu Val Gln Tyr Gln Gln Gly Ala Asp Val Asn Lys Met50 55 60cgc gaa cgc ctc gat acg ctg aaa ata ggt gat gta cag gtt cag gca240Arg Glu Arg Leu Asp Thr Leu Lys Ile Gly Asp Val Gln Val Gln Ala65 70 75 80ttg ggt acg aac aaa cac atc atg atc cgc ctg ccg aac aaa gaa ggt288Leu Gly Thr Asn Lys His Ile Met Ile Arg Leu Pro Asn Lys Glu Gly85 90 95gtt act tcc gca cag ttg tcc aat cag gtt atg gat ttg ctg aaa aaa336Val Thr Ser Ala Gln Leu Ser Asn Gln Val Met Asp Leu Leu Lys Lys100 105 110gac agt ccc gac gtt acc ttg cgc caa gtc gaa ttt atc ggc ccg caa384Asp Ser Pro Asp Val Thr Leu Arg Gln Val Glu Phe Ile Gly Pro Gln115 120 125gtc ggt gag gaa ttg gta agt aat gga ttg atg gct tta ggt ttt gtc432Val Gly Glu Glu Leu Val Ser Asn Gly Leu Met Ala Leu Gly Phe Val130 135 140gtt atc ggc atc att att tac ctg tcg atg cgt ttt gaa tgg cgt ttt480Val Ile Gly Ile Ile Ile Tyr Leu Ser Met Arg Phe Glu Trp Arg Phe145 150 155 160gcc gta tct gcc att atc gcc aat atg cac gac atc gtg att att ctc528Ala Val Ser Ala Ile Ile Ala Asn Met His Asp Ile Val Ile Ile Leu165 170 175
ggc tgc ttt gcc ttc ttc caa tgg gaa ttt tcg ctg acc gtc ttg gcg576Gly Cys Phe Ala Phe Phe Gln Trp Glu Phe Ser Leu Thr Val Leu Ala180 185 190ggt atc ctt gcc gta ttg ggc tat tct gtg aac gaa tcc gtc gtc gtc624Gly Ile Leu Ala Val Leu Gly Tyr Ser Val Asn Glu Ser Val Val Val195 200 205ttc gac cgt atc cgt gaa aac ttc cgc aag ccg gcg atg cgc gga cat672Phe Asp Arg Ile Arg Glu Asn Phe Arg Lys Pro Ala Met Arg Gly His210 215 220gcc gtg ccg gaa gtc atc gac aac gcg att acc gca acg atg agc cgc720Ala Val Pro Glu Val Ile Asp Asn Ala Ile Thr Ala Thr Met Ser Arg225 230 235 240acc atc att acc cac ggt tcg acc gag gcg atg gtc gta tcc atg ctg768Thr Ile Ile Thr His Gly Ser Thr Glu Ala Met Val Val Ser Met Leu245 250 255gtg ttc ggc ggt gcg gcc ttg cac ggc ttt tct atg gcg ttg acc att816Val Phe Gly Gly Ala Ala Leu His Gly Phe Ser Met Ala Leu Thr Ile260 265 270ggc atc gtg ttc ggc att tat tct tcc gta ttg gtt gcc agc ccg ctt864Gly Ile Val Phe Gly Ile Tyr Ser Ser Val Leu Val Ala Ser Pro Leu275 280 285ctg cta atg ttc ggt ttg agc cgc gac aat atc ggt aaa gaa ccg aag912Leu Leu Met Phe Gly Leu Ser Arg Asp Asn Ile Gly Lys Glu Pro Lys290 295 300aag aaa gaa gaa atc gtg gtt tga936Lys Lys Glu Glu Ile Val Val305 310<210>6<211>311<212>PRT<213>脑膜炎奈瑟球菌<400>6Met Glu Leu Phe Lys Ile Lys Arg Asp Ile Pro Phe Met Ser Tyr Gly1 5 10 15Lys Leu Thr Thr Phe Ile Ser Leu Val Thr Phe Ile Ala Ala Val Phe20 25 30
Phe Leu Val Thr Arg Gly Leu Asn Phe Ser Val Glu Phe Thr Gly Gly35 40 45Thr Val Met Glu Val Gln Tyr Gln Gln Gly Ala Asp Val Asn Lys Met50 55 60Arg Glu Arg Leu Asp Thr Leu Lys Ile Gly Asp Val Gln Val Gln Ala65 70 75 80Leu Gly Thr Asn Lys His Ile Met Ile Arg Leu Pro Asn Lys Glu Gly85 90 95Val Thr Ser Ala Gln Leu Ser Asn Gln Val Met Asp Leu Leu Lys Lys100 105 110Asp Ser Pro Asp Val Thr Leu Arg Gln Val Glu Phe Ile Gly Pro Gln115 120 125Val Gly Glu Glu Leu Val Ser Asn Gly Leu Met Ala Leu Gly Phe Val130 135 140Val Ile Gly Ile Ile Ile Tyr Leu Ser Met Arg Phe Glu Trp Arg Phe145 150 155 160Ala Val Ser Ala Ile Ile Ala Asn Met His Asp Ile Val Ile Ile Leu165 170 175Gly Cys Phe Ala Phe Phe Gln Trp Glu Phe Ser Leu Thr Val Leu Ala180 185 190Gly Ile Leu Ala Val Leu Gly Tyr Ser Val Asn Glu Ser Val Val Val195 200 205Phe Asp Arg Ile Arg Glu Asn Phe Arg Lys Pro Ala Met Arg Gly His210 215 220Ala Val Pro Glu Val Ile Asp Asn Ala Ile Thr Ala Thr Met Ser Arg225 230 235 240Thr Ile Ile Thr His Gly Ser Thr Glu Ala Met Val Val Ser Met Leu245 250 255Val Phe Gly Gly Ala Ala Leu His Gly Phe Ser Met Ala Leu Thr Ile260 265 270Gly Ile Val Phe Gly Ile Tyr Ser Ser Val Leu Val Ala Ser Pro Leu275 280 285
Leu Leu Met Phe Gly Leu Ser Arg Asp Asn Ile Gly Lys Glu Pro Lys290 295 300Lys Lys Glu Glu Ile Val Val305 310<210>7<211>2277<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(2277)<400>7gtg tcc gaa ccc ata cag cct acc agc ctg agc ctc ggt tcg acc tgc48Val Ser Glu Pro Ile Gln Pro Thr Ser Leu Ser Leu Gly Ser Thr Cys1 5 10 15ctg ttt tgc agt aac gaa agc ggc agc ccc gag aga acc gaa gcc gcc96Leu Phe Cys Ser Asn Glu Ser Gly Ser Pro Glu Arg Thr Glu Ala Ala20 25 30gtc caa ggc agc ggc gaa gca tcc atc ccc gaa gac tat acg cgc att144Val Gln Gly Ser Gly Glu Ala Ser Ile Pro Glu Asp Tyr Thr Arg Ile35 40 45gtt gcc gac agg atg gaa gga cag tcg cag gtg cag gtg cgt gcc gaa192Val Ala Asp Arg Met Glu Gly Gln Ser Gln Val Gln Val Arg Ala Glu50 55 60ggc aac gtc gtc gtc gaa cgc aac cgg acg acc ctc aat acc gat tgg240Gly Asn Val Val Val Glu Arg Asn Arg Thr Thr Leu Asn Thr Asp Trp65 70 75 80gcg gat tac gac cag tcg ggc gac acc gtt acc gca ggc gac cgg ttc288Ala Asp Tyr Asp Gln Ser Gly Asp Thr Val Thr Ala Gly Asp Arg Phe85 90 95gcc ctc caa cag gac ggt acg ctg att cgg ggc gaa acc ctg acc tac336Ala Leu Gln Gln Asp Gly Thr Leu Ile Arg Gly Glu Thr Leu Thr Tyr100 105 110aat ctc gag cag cag acc ggg gaa gcg cac aac gtc cgc atg gaa atc384
Asn Leu Glu Gln Gln Thr Gly Glu Ala His Asn Val Arg Met Glu Ile115 120 125gaa caa ggc gga cgg cgg ctg caa agc gtc agc cgc acc gcc gaa atg432Glu Gln Gly Gly Arg Arg Leu Gln Ser Val Ser Arg Thr Ala Glu Met130 135 140ttg ggc gaa ggg cat tac aaa ctg acg gaa acc caa ttc aac acc tgt480Leu Gly Glu Gly His Tyr Lys Leu Thr Glu Thr Gln Phe Asn Thr Cys145 150 155 160tcc gcc ggc gat gcc ggc tgg tat gtc aag gca gcc tct gtc gaa gcc528Ser Ala Gly Asp Ala Gly Trp Tyr Val Lys Ala Ala Ser Val Glu Ala165 170 175gat cgg gaa aaa ggc ata ggc gtt gcc aaa cac gcc gcc ttc gtg ttc576Asp Arg Glu Lys Gly Ile Gly Val Ala Lys His Ala Ala Phe Val Phe180 185 190ggc ggc gtt ccc att ttc tac acc cct tgg gcg gac ttc ccg ctt gac624Gly Gly Val Pro Ile Phe Tyr Thr Pro Trp Ala Asp Phe Pro Leu Asp195 200 205ggc aac cgc aaa agc ggc ctg ctt gtt ccc tca ctg tcc gcc ggt tcg672Gly Asn Arg Lys Ser Gly Leu Leu Val Pro Ser Leu Ser Ala Gly Ser210 215 220gac ggc gtt tcc ctt tcc gtt ccc tat tat ttc aac ctt gcc ccc aat720Asp Gly Val Ser Leu Ser Val Pro Tyr Tyr Phe Asn Leu Ala Pro Asn225 230 235 240ctc gat gcc acg ttc gcg ccc agc gtg atc ggc gaa cgc ggc gcg gtc768Leu Asp Ala Thr Phe Ala Pro Ser Val Ile Gly Glu Arg Gly Ala Val245 250 255ttt gac ggg cag gta cgc tac ctg cgg ccg gat tat gcc ggc cag tcc816Phe Asp Gly Gln Val Arg Tyr Leu Arg Pro Asp Tyr Ala Gly Gln Ser260 265 270gac ctg acc tgg ctg ccg cac gac aag aaa agc ggc agg aat aac cgc864Asp Leu Thr Trp Leu Pro His Asp Lys Lys Ser Gly Arg Asn Asn Arg275 280 285tat cag gcg aaa tgg cag cat cgg cac gac att tcc gac acg ctt cag912Tyr Gln Ala Lys Trp Gln His Arg His Asp Ile Ser Asp Thr Leu Gln290 295 300gcg ggt gtc gat ttc aac caa gtc tcc gac agc ggc tac tac cgc gac960
Ala Gly Val Asp Phe Asn Gln Val Ser Asp Ser Gly Tyr Tyr Arg Asp305 310 315 320ttt tac ggc aac aaa gaa atc gcc ggc aac gtc aac ctc aac cgc cgt1008Phe Tyr Gly Asn Lys Glu Ile Ala Gly Asn Val Asn Leu Asn Arg Arg325 330 335gta tgg ctg gat tat ggc ggc agg gcg gcg ggc ggc agc ctg aat gcc1056Val Trp Leu Asp Tyr Gly Gly Arg Ala Ala Gly Gly Ser Leu Asn Ala340 345 350ggc ctt tcg gtt ctg aaa tac cag acg ctg gca aac caa agc ggc tac1104Gly Leu Ser Val Leu Lys Tyr Gln Thr Leu Ala Asn Gln Ser Gly Tyr355 360 365aaa gac aaa ccg tat gcc ctc atg ccg cgc ctt tcg gtc gag tgg cgt1152Lys Asp Lys Pro Tyr Ala Leu Met Pro Arg Leu Ser Val Glu Trp Arg370 375 380aaa aac acc ggc agg gcg caa atc ggc gtg tcc gca caa ttt acc cga1200Lys Asn Thr Gly Arg Ala Gln Ile Gly Val Ser Ala Gln Phe Thr Arg385 390 395 400ttc agc cac gac agc cgc caa gac ggc agc cgc ctg gtc gtc tat ccc1248Phe Ser His Asp Ser Arg Gln Asp Gly Ser Arg Leu Val Val Tyr Pro405 410 415gac atc aaa tgg gat ttc agc aac agc tgg ggc tat gtc cgt ccc aaa1296Asp Ile Lys Trp Asp Phe Ser Asn Ser Trp Gly Tyr Val Arg Pro Lys420 425 430ctc gga ctg cac gcc acc tat tac agc ctc aac cgc ttc ggc agc caa1344Leu Gly Leu His Ala Thr Tyr Tyr Ser Leu Asn Arg Phe Gly Ser Gln435 440 445gaa gcc cga cgc gtc agc cgc act ctg ccc att gtc aac atc gac agc1392Glu Ala Arg Arg Val Ser Arg Thr Leu Pro Ile Val Asn Ile Asp Ser450 455 460ggc gca act ttt gag cgg aat acg cgg atg ttc ggc gga gaa gtc ctg1440Gly Ala Thr Phe Glu Arg Asn Thr Arg Met Phe Gly Gly Glu Val Leu465 470 475 480caa acc ctc gag ccg cgc ctg ttc tac aac tat att cct gcc aaa tcc1488Gln Thr Leu Glu Pro Arg Leu Phe Tyr Asn Tyr Ile Pro Ala Lys Ser485 490 495caa aac gac ctg ccc aat ttc gat tcg tcg gaa agc agc ttc ggc tac1536
Gln Asn Asp Leu Pro Asn Phe Asp Ser Ser Glu Ser Ser Phe Gly Tyr500 505 510ggg cag ctc ttt cgc gaa aac ctc tat tac ggc aac gac agg att aac1584Gly Gln Leu Phe Arg Glu Asn Leu Tyr Tyr Gly Asn Asp Arg Ile Asn515 520 525acc gca aac agc ctt tcc gcc gcc gtg caa agc cgt att ttg gac ggc1632Thr Ala Asn Ser Leu Ser Ala Ala Val Gln Ser Arg Ile Leu Asp Gly530 535 540gcg acg ggg gaa gag cgt ttc cgc gcc ggc atc ggt cag aaa ttc tat1680Ala Thr Gly Glu Glu Arg Phe Arg Ala Gly Ile Gly Gln Lys Phe Tyr545 550 555 560ttc aag gat gat gcg gtg atg ctt gac ggc agc gtc ggc aaa aaa ccg1728Phe Lys Asp Asp Ala Val Met Leu Asp Gly Ser Val Gly Lys Lys Pro565 570 575cgc aac cgt tcc gac tgg gtg gca ttt gcc tcc ggc agc atc ggc agc1776Arg Asn Arg Ser Asp Trp Val Ala Phe Ala Ser Gly Ser Ile Gly Ser580 585 590cgc ttc atc ctc gac agc agc atc cac tac aac caa aac gac aaa cgc1824Arg Phe Ile Leu Asp Ser Ser Ile His Tyr Asn Gln Asn Asp Lys Arg595 600 605gcc gag aac tac gcc gtc ggt gca agc tac cgt ccc gca cag ggc aaa1872Ala Glu Asn Tyr Ala Val Gly Ala Ser Tyr Arg Pro Ala Gln Gly Lys610 615 620gtg ctg aac gcc cgc tac aaa tac ggg cgc aac gaa aaa atc tac ctg1920Val Leu Asn Ala Arg Tyr Lys Tyr Gly Arg Asn Glu Lys Ile Tyr Leu625 630 635 640aag tcc gac ggt tcc tat ttt tac gac aaa ctc agc cag ctc gac ctg1968Lys Ser Asp Gly Ser Tyr Phe Tyr Asp Lys Leu Ser Gln Leu Asp Leu645 650 655tcc gca caa tgg ccg ctg acg cgc aac ctg tcg gcc gtc gtc cgt tac2016Ser Ala Gln Trp Pro Leu Thr Arg Asn Leu Ser Ala Val Val Arg Tyr660 665 670aac tac ggt ttt gaa gcc aaa aaa ccg ata gag gtg ctg gcg ggt gcg2064Asn Tyr Gly Phe Glu Ala Lys Lys Pro Ile Glu Val Leu Ala Gly Ala675 680 685gaa tac aaa agc agt tgc ggc tgc tgg ggc gcg ggc gtg tac gcc caa2112
Glu Tyr Lys Ser Ser Cys Gly Cys Trp Gly Ala Gly Val Tyr Ala Gln690 695 700cgc tac gtt acc ggc gaa aac acc tac aaa aac gct gtc ttt ttc tca2160Arg Tyr Val Thr Gly Glu Asn Thr Tyr Lys Asn Ala Val Phe Phe Ser705 710 715 720ctt cag ttg aaa gac ctc agc agt gtc ggc aga aac ccc gca gac agg2208Leu Gln Leu Lys Asp Leu Ser Ser Val Gly Arg Asn Pro Ala Asp Arg725 730 735atg gat gtc gcc gtt ccc ggc tat atc acc gcc cac tct ctt tcc gcc2256Met Asp Val Ala Val Pro Gly Tyr Ile Thr Ala His Ser Leu Ser Ala740 745 750gga cgc aac aaa cga ccc tga2277Gly Arg Asn Lys Arg Pro755<210>8<211>758<212>PRT<213>脑膜炎奈瑟球菌<400>8Val Ser Glu Pro Ile Gln Pro Thr Ser Leu Ser Leu Gly Ser Thr Cys1 5 10 15Leu Phe Cys Ser Asn Glu Ser Gly Ser Pro Glu Arg Thr Glu Ala Ala20 25 30Val Gln Gly Ser Gly Glu Ala Ser Ile Pro Glu Asp Tyr Thr Arg Ile35 40 45Val Ala Asp Arg Met Glu Gly Gln Ser Gln Val Gln Val Arg Ala Glu50 55 60Gly Asn Val Val Val Glu Arg Asn Arg Thr Thr Leu Asn Thr Asp Trp65 70 75 80Ala Asp Tyr Asp Gln Ser Gly Asp Thr Val Thr Ala Gly Asp Arg Phe85 90 95Ala Leu Gln Gln Asp Gly Thr Leu Ile Arg Gly Glu Thr Leu Thr Tyr100 105 110Asn Leu Glu Gln Gln Thr Gly Glu Ala His Asn Val Arg Met Glu Ile
115 120 125Glu Gln Gly Gly Arg Arg Leu Gln Ser Val Ser Arg Thr Ala Glu Met130 135 140Leu Gly Glu Gly His Tyr Lys Leu Thr Glu Thr Gln Phe Asn Thr Cys145 150 155 160Ser Ala Gly Asp Ala Gly Trp Tyr Val Lys Ala Ala Ser Val Glu Ala165 170 175Asp Arg Glu Lys Gly Ile Gly Val Ala Lys His Ala Ala Phe Val Phe180 185 190Gly Gly Val Pro Ile Phe Tyr Thr Pro Trp Ala Asp Phe Pro Leu Asp195 200 205Gly Asn Arg Lys Ser Gly Leu Leu Val Pro Ser Leu Ser Ala Gly Ser210 215 220Asp Gly Val Ser Leu Ser Val Pro Tyr Tyr Phe Asn Leu Ala Pro Asn225 230 235 240Leu Asp Ala Thr Phe Ala Pro Ser Val Ile Gly Glu Arg Gly Ala Val245 250 255Phe Asp Gly Gln Val Arg Tyr Leu Arg Pro Asp Tyr Ala Gly Gln Ser260 265 270Asp Leu Thr Trp Leu Pro His Asp Lys Lys Ser Gly Arg Asn Asn Arg275 280 285Tyr Gln Ala Lys Trp Gln His Arg His Asp Ile Ser Asp Thr Leu Gln290 295 300Ala Gly Val Asp Phe Asn Gln Val Ser Asp Ser Gly Tyr Tyr Arg Asp305 310 315 320Phe Tyr Gly Asn Lys Glu Ile Ala Gly Asn Val Asn Leu Asn Arg Arg325 330 335Val Trp Leu Asp Tyr Gly Gly Arg Ala Ala Gly Gly Ser Leu Asn Ala340 345 350Gly Leu Ser Val Leu Lys Tyr Gln Thr Leu Ala Asn Gln Ser Gly Tyr355 360 365Lys Asp Lys Pro Tyr Ala Leu Met Pro Arg Leu Ser Val Glu Trp Arg
370 375 380Lys Asn Thr Gly Arg Ala Gln Ile Gly Val Ser Ala Gln Phe Thr Arg385 390 395 400Phe Ser His Asp Ser Arg Gln Asp Gly Ser Arg Leu Val Val Tyr Pro405 410 415Asp Ile Lys Trp Asp Phe Ser Asn Ser Trp Gly Tyr Val Arg Pro Lys420 425 430Leu Gly Leu His Ala Thr Tyr Tyr Ser Leu Asn Arg Phe Gly Ser Gln435 440 445Glu Ala Arg Arg Val Ser Arg Thr Leu Pro Ile Val Asn Ile Asp Ser450 455 460Gly Ala Thr Phe Glu Arg Asn Thr Arg Met Phe Gly Gly Glu Val Leu465 470 475 480Gln Thr Leu Glu Pro Arg Leu Phe Tyr Asn Tyr Ile Pro Ala Lys Ser485 490 495Gln Asn Asp Leu Pro Asn Phe Asp Ser Ser Glu Ser Ser Phe Gly Tyr500 505 510Gly Gln Leu Phe Arg Glu Asn Leu Tyr Tyr Gly Asn Asp Arg Ile Asn515 520 525Thr Ala Asn Ser Leu Ser Ala Ala Val Gln Ser Arg Ile Leu Asp Gly530 535 540Ala Thr Gly Glu Glu Arg Phe Arg Ala Gly Ile Gly Gln Lys Phe Tyr545 550 555 560Phe Lys Asp Asp Ala Val Met Leu Asp Gly Ser Val Gly Lys Lys Pro565 570 575Arg Asn Arg Ser Asp Trp Val Ala Phe Ala Ser Gly Ser Ile Gly Ser580 585 590Arg Phe Ile Leu Asp Ser Ser Ile His Tyr Asn Gln Asn Asp Lys Arg595 600 605Ala Glu Asn Tyr Ala Val Gly Ala Ser Tyr Arg Pro Ala Gln Gly Lys610 615 620Val Leu Asn Ala Arg Tyr Lys Tyr Gly Arg Asn Glu Lys Ile Tyr Leu
625 630 635 640Lys Ser Asp Gly Ser Tyr Phe Tyr Asp Lys Leu Ser Gln Leu Asp Leu645 650 655Ser Ala Gln Trp Pro Leu Thr Arg Asn Leu Ser Ala Val Val Arg Tyr660 665 670Asn Tyr Gly Phe Glu Ala Lys Lys Pro Ile Glu Val Leu Ala Gly Ala675 680 685Glu Tyr Lys Ser Ser Cys Gly Cys Trp Gly Ala Gly Val Tyr Ala Gln690 695 700Arg Tyr Val Thr Gly Glu Asn Thr Tyr Lys Asn Ala Val Phe Phe Ser705 710 715 720Leu Gln Leu Lys Asp Leu Ser Ser Val Gly Arg Asn Pro Ala Asp Arg725 730 735Met Asp Val Ala Val Pro Gly Tyr Ile Thr Ala His Ser Leu Ser Ala740 745 750Gly Arg Asn Lys Arg Pro755<210>9<211>939<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(939)<400>9atg tcc atc cat act ctg aaa cgc ctg ccc tca tcg ctg ctg ctc ggt48Met Ser Ile His Thr Leu Lys Arg Leu Pro Ser Ser Leu Leu Leu Gly1 5 10 15ctc tgc ctt tcc ctg ccg tca gcc cac ctt ttt gcc gac aac gac att96Leu Cys Leu Ser Leu Pro Ser Ala His Leu Phe Ala Asp Asn Asp Ile20 25 30tta ggg caa ttt tta gaa cag aac atg ctt acc tcc tcc gat ccg ata144Leu Gly Gln Phe Leu Glu Gln Asn Met Leu Thr Ser Ser Asp Pro Ile
35 40 45gaa ata ttc gcc gaa agc acg ata cac ccc acc aac acc caa gcc att192Glu Ile Phe Ala Glu Ser Thr Ile His Pro Thr Asn Thr Gln Ala Ile50 55 60aca ggc ggt ctg att ctc tcc tca cag tct gcc ctg gtc gtc aac aac240Thr Gly Gly Leu Ile Leu Ser Ser Gln Ser Ala Leu Val Val Asn Asn65 70 75 80aaa acc gga cag ata ctg tat cag aaa aac gcc gac agg att atg ccc288Lys Thr Gly Gln Ile Leu Tyr Gln Lys Asn Ala Asp Arg Ile Met Pro85 90 95atc gcc tcc att tcc aaa ctg atg agc gcg atg gtc gtt ttg gat gca336Ile Ala Ser Ile Ser Lys Leu Met Ser Ala Met Val Val Leu Asp Ala100 105 110aac ttg gac atg aac gaa acc gtt acc att acg ccc gac gaa atc gac384Asn Leu Asp Met Asn Glu Thr Val Thr Ile Thr Pro Asp Glu Ile Asp115 120 125cgc atc aaa ggg acc ggc agc cgt ctt gcc ata ggt acg gca ctt aca432Arg Ile Lys Gly Thr Gly Ser Arg Leu Ala Ile Gly Thr Ala Leu Thr130 135 140cgc aaa aaa ctg ctg cac ctg agc ctg atg agc agc gaa aac cgc gcc480Arg Lys Lys Leu Leu His Leu Ser Leu Met Ser Ser Glu Asn Arg Ala145 150 155 160acc cat gca ttg ggc aga acc tac ccc ggc ggc atg ggc gca ttt gtc528Thr His Ala Leu Gly Arg Thr Tyr Pro Gly Gly Met Gly Ala Phe Val165 170 175gcc gcc atg aac cgc aaa gcc caa agc ctc ggt atg tac ggc agc cgc576Ala Ala Met Asn Arg Lys Ala Gln Ser Leu Gly Met Tyr Gly Ser Arg180 185 190ttt tac gaa ccg acc gga ctc aac ttc caa aac gtt tct acc gcc aaa624Phe Tyr Glu Pro Thr Gly Leu Asn Phe Gln Asn Val Ser Thr Ala Lys195 200 205gac ctg agc ctt atg gtc aac gcc gcc gcc caa tat ccg caa atc cgc672Asp Leu Ser Leu Met Val Asn Ala Ala Ala Gln Tyr Pro Gln Ile Arg210 215 220acc aac tcg act tcc aac tac gcc tcg gta cag acc aaa aac ggg cag720Thr Asn Ser Thr Ser Asn Tyr Ala Ser Val Gln Thr Lys Asn Gly Gln
225 230 235 240cag aac tac aaa aac tcc aat gcc ctg gtc aga gaa ggc atg tgg aac768Gln Asn Tyr Lys Asn Ser Asn Ala Leu Val Arg Glu Gly Met Trp Asn245 250 255atc gaa ttg cag aaa acc ggc tac ata cgc gaa gca ggc agg tct atg816Ile Glu Leu Gln Lys Thr Gly Tyr Ile Arg Glu Ala Gly Arg Ser Met260 265 270gtt gtc aaa gcc aac att caa aac caa ccc gtt acc atc gta ttg ctg864Val Val Lys Ala Asn Ile Gln Asn Gln Pro Val Thr Ile Val Leu Leu275 280 285aac tcg ccc aca tcc gcc aca cgc gtc aac gac gcc cgc aaa atc gaa912Asn Ser Pro Thr Ser Ala Thr Arg Val Asn Asp Ala Arg Lys Ile Glu290 295 300tcg tgg atg ctg cag caa cgc tcc tga939Ser Trp Met Leu Gln Gln Arg Ser305 310<210>10<211>312<212>PRT<213>脑膜炎奈瑟球菌<400>10Met Ser Ile His Thr Leu Lys Arg Leu Pro Ser Ser Leu Leu Leu Gly1 5 10 15Leu cys Leu Ser Leu Pro Ser Ala His Leu Phe Ala Asp Asn Asp Ile20 25 30Leu Gly Gln Phe Leu Glu Gln Asn Met Leu Thr Ser Ser Asp Pro Ile35 40 45Glu Ile Phe Ala Glu Ser Thr Ile His Pro Thr Asn Thr Gln Ala Ile50 55 60Thr Gly Gly Leu Ile Leu Ser Ser Gln Ser Ala Leu Val Val Asn Asn65 70 75 80Lys Thr Gly Gln Ile Leu Tyr Gln Lys Asn Ala Asp Arg Ile Met Pro85 90 95Ile Ala Ser Ile Ser Lys Leu Met Ser Ala Met Val Val Leu Asp Ala
100 105 110Asn Leu Asp Met Asn Glu Thr Val Thr Ile Thr Pro Asp Glu Ile Asp115 120 125Arg Ile Lys Gly Thr Gly Ser Arg Leu Ala Ile Gly Thr Ala Leu Thr130 135 140Arg Lys Lys Leu Leu His Leu Ser Leu Met Ser Ser Glu Asn Arg Ala145 150 155 160Thr His Ala Leu Gly Arg Thr Tyr Pro Gly Gly Met Gly Ala Phe Val165 170 175Ala Ala Met Asn Arg Lys Ala Gln Ser Leu Gly Met Tyr Gly Ser Arg180 185 190Phe Tyr Glu Pro Thr Gly Leu Asn Phe Gln Asn Val Ser Thr Ala Lys195 200 205Asp Leu Ser Leu Met Val Asn Ala Ala Ala Gln Tyr Pro Gln Ile Arg210 215 220Thr Asn Ser Thr Ser Asn Tyr Ala Ser Val Gln Thr Lys Asn Gly Gln225 230 235 240Gln Asn Tyr Lys Asn Ser Asn Ala Leu Val Arg Glu Gly Met Trp Asn245 250 255Ile Glu Leu Gln Lys Thr Gly Tyr Ile Arg Glu Ala Gly Arg Ser Met260 265 270Val Val Lys Ala Asn Ile Gln Asn Gln Pro Val Thr Ile Val Leu Leu275 280 285Asn Ser Pro Thr Ser Ala Thr Arg Val Asn Asp Ala Arg Lys Ile Glu290 295 300Ser Trp Met Leu Gln Gln Arg Ser305 310<210>11<211>1806<212>DNA<213>脑膜炎奈瑟球菌
<220>
<221>CDS<222>(1)..(1806)<400>11atg aaa aaa ctg att tgc ctg ttc gcc gta ttt ttg atg ttg tgc gga48Met Lys Lys Leu Ile Cys Leu Phe Ala Val Phe Leu Met Leu Cys Gly1 5 10 15cga gct ttc gcg ctg gat gcg aac gat ctg ctg ccg ccg gaa aag gca96Arg Ala Phe Ala Leu Asp Ala Asn Asp Leu Leu Pro Pro Glu Lys Ala20 25 30ttc gtg ccg gag ctt gcc gtt gcc gac gac ggt gtg aac gtc cgt ttc144Phe Val Pro Glu Leu Ala Val Ala Asp Asp Gly Val Asn Val Arg Phe35 40 45agg att gcc gac gga tac tat atg tat cag gcg aaa atc gtc ggc aag192Arg Ile Ala Asp Gly Tyr Tyr Met Tyr Gln Ala Lys Ile Val Gly Lys50 55 60acc gat ccg gcg gat ttg ttg gga cag cct tct ttc agc aag ggc gaa240Thr Asp Pro Ala Asp Leu Leu Gly Gln Pro Ser Phe Ser Lys Gly Glu65 70 75 80gag aag gaa gac gag ttt ttc ggc agg cag acg gtt tac cat cac gag288Glu Lys Glu Asp Glu Phe Phe Gly Arg Gln Thr Val Tyr His His Glu85 90 95gcg cag gtt gcc ttt cct tat gca aag gct gtc ggc gaa ccg tat aaa336Ala Gln Val Ala Phe Pro Tyr Ala Lys Ala Val Gly Glu Pro Tyr Lys100 105 110ttg gtt ttg acc tat cag ggc tgt gcc gaa gcc ggc gtg tgc tat ccg384Leu Val Leu Thr Tyr Gln Gly Cys Ala Glu Ala Gly Val Cys Tyr Pro115 120 125ccc gtg gat acc gag ttt gat att ttc ggc aac ggc act tac cat ccg432Pro Val Asp Thr Glu Phe Asp Ile Phe Gly Asn Gly Thr Tyr His Pro130 135 140caa acc gac gaa ccg gca tcc gcc aaa gac cgc ttt ttg cag cct tcc480Gln Thr Asp Glu Pro Ala Ser Ala Lys Asp Arg Phe Leu Gln Pro Ser145 150 155 160tct caa aac ggc agc ggg gcg ctg ccg ccc ccg aag ggg gat gag ggc528Ser Gln Asn Gly Ser Gly Ala Leu Pro Pro Pro Lys Gly Asp Glu Gly165 170 175
ggc gac agc cgt ttc aag ctg tct tgg gat acg ctc aac gcc aat ctt576Gly Asp Ser Arg Phe Lys Leu Ser Trp Asp Thr Leu Asn Ala Asn Leu180 185 190ttg gcg ttt ttt ctc gct ggt ttg ggc ctg agt ttt acc gcc tgt atg624Leu Ala Phe Phe Leu Ala Gly Leu Gly Leu Ser Phe Thr Ala Cys Met195 200 205tat ccc ctg ttg ccg att gtt tcc agt att gtg gtc ggc gac aaa aag672Tyr Pro Leu Leu Pro Ile Val Ser Ser Ile Val Val Gly Asp Lys Lys210 215 220gcg ggc aag gcg cgg gcg ttt gtg ctg tcc gtc gtt tat gtt cag ggt720Ala Gly Lys Ala Arg Ala Phe Val Leu Ser Val Val Tyr Val Gln Gly225 230 235 240ttg gct ctg act tat acg ctg gtc ggc att gtt gcc gga ctg acg ggc768Leu Ala Leu Thr Tyr Thr Leu Val Gly Ile Val Ala Gly Leu Thr Gly245 250 255gca ctg ctg acc gta tgg ttg cag cag gct tgg gtg gta ttg gcg gca816Ala Leu Leu Thr Val Trp Leu Gln Gln Ala Trp Val Val Leu Ala Ala260 265 270tcg gct tta atg gtc gtc ttg gca ctg tct atg ttc ggg ctg ttc aac864Ser Ala Leu Met Val Val Leu Ala Leu Ser Met Phe Gly Leu Phe Asn275 280 285atc cag ctt ccc aac gcc gtg cag tcg tat ttt cag aat caa agc agc912Ile Gln Leu Pro Asn Ala Val Gln Ser Tyr Phe Gln Asn Gln Ser Ser290 295 300agg ctt tca ggc ggt aaa atc gtt tcc gtc ttt att atg ggc ata ttg960Arg Leu Ser Gly Gly Lys Ile Val Ser Val Phe Ile Met Gly Ile Leu305 310 315 320tcc gcg ctg att gtc ggg ccg tgc gtc gcc ccg ccg ctg gca ttt gct1008Ser Ala Leu Ile Val Gly Pro Cys Val Ala Pro Pro Leu Ala Phe Ala325 330 335ttg ggc tac atc ggt cag acg ggc gat gcg gtt tta ggc ggt ttg gca1056Leu Gly Tyr Ile Gly Gln Thr Gly Asp Ala Val Leu Gly Gly Leu Ala340 345 350ctt tac act ttg gcg ttg ggc acc ggc gtt ccg ctg att gcc atc ggc1104Leu Tyr Thr Leu Ala Leu Gly Thr Gly Val Pro Leu Ile Ala Ile Gly355 360 365
acg ttc ggc ggg cat atc ctg cct aag gca ggc gat tgg atg aat gcc1152Thr Phe Gly Gly His Ile Leu Pro Lys Ala Gly Asp Trp Met Asn Ala370 375 380gtc aaa tac gca ttc ggc ttc atc ctg cta gcc gtc gcc gtt tac ctc1200Val Lys Tyr Ala Phe Gly Phe Ile Leu Leu Ala Val Ala Val Tyr Leu385 390 395 400gcc acg ccg cac ttg ccc tat tat ctc gtc gtc gcg ctg tac acg ctg1248Ala Thr Pro His Leu Pro Tyr Tyr Leu Val Val Ala Leu Tyr Thr Leu405 410 415ctg atg ctg gtt cct gcc ttt atg ctg ctg gtc aac gga cgc agg cag1296Leu Met Leu Val Pro Ala Phe Met Leu Leu Val Asn Gly Arg Arg Gln420 425 430aaa cgc cgt ccg aaa gct gtg gca ttc gca ttg ggc ggt ata ttg ctg1344Lys Arg Arg Pro Lys Ala Val Ala Phe Ala Leu Gly Gly Ile Leu Leu435 440 445ata ggc ggc gcg tgg ttc ggc tgg cag ggc gca aac ggc aaa acg acc1392Ile Gly Gly Ala Trp Phe Gly Trp Gln Gly Ala Asn Gly Lys Thr Thr450 455 460gcg ctg cac cat ttc ctg acc ctc aat cca cca gcc gaa gca ggc aaa1440Ala Leu His His Phe Leu Thr Leu Asn Pro Pro Ala Glu Ala Gly Lys465 470 475 480tct tcg gaa cac ggc aaa atg ttt gcc gat act gcc gcg ctg aag gca1488Ser Ser Glu His Gly Lys Met Phe Ala Asp Thr Ala Ala Leu Lys Ala485 490 495gcg atg gat acg gcg ttg aaa gaa cat ccc gac aaa ccc gtc gtt ttg1536Ala Met Asp Thr Ala Leu Lys Glu His Pro Asp Lys Pro Val Val Leu500 505 510gat ttt tat gcc gac tgg tgc att tcc tgc aaa gaa atg gcg gct tac1584Asp Phe Tyr Ala Asp Trp Cys Ile Ser Cys Lys Glu Met Ala Ala Tyr515 520 525acg ctc aat cag ccg gaa gtg cat cag gca gtc gat atg gaa cgc ttt1632Thr Leu Asn Gln Pro Glu Val His Gln Ala Val Asp Met Glu Arg Phe530 535 540ttc caa atc gac gta acc gcc aac acg ccc gaa cat cag gcg ttg ttg1680Phe Gln Ile Asp Val Thr Ala Asn Thr Pro Glu His Gln Ala Leu Leu545 550 555 560
aaa gaa tac ggt ctg ttc ggg ccg ccg ggc gtg ttt gtc gtc cgc tcc1728Lys Glu Tyr Gly Leu Phe Gly Pro Pro Gly Val Phe Val Val Arg Ser565 570 575gac ggc agc cgc agc gag ccg ctg ctg ggt ttt gtc aaa gca gac aag1776Asp Gly Ser Arg Ser Glu Pro Leu Leu Gly Phe Val Lys Ala Asp Lys580 585 590ttt atc gag tgg tat gaa caa aac cgc tga1806Phe Ile Glu Trp Tyr Glu Gln Asn Arg595 600<210>12<211>601<212>PRT<213>脑膜炎奈瑟球菌<400>12Met Lys Lys Leu Ile Cys Leu Phe Ala Val Phe Leu Met Leu Cys Gly1 5 10 15Arg Ala Phe Ala Leu Asp Ala Asn Asp Leu Leu Pro Pro Glu Lys Ala20 25 30Phe Val Pro Glu Leu Ala Val Ala Asp Asp Gly Val Asn Val Arg Phe35 40 45Arg Ile Ala Asp Gly Tyr Tyr Met Tyr Gln Ala Lys Ile Val Gly Lys50 55 60Thr Asp Pro Ala Asp Leu Leu Gly Gln Pro Ser Phe Ser Lys Gly Glu65 70 75 80Glu Lys Glu Asp Glu Phe Phe Gly Arg Gln Thr Val Tyr His His Glu85 90 95Ala Gln Val Ala Phe Pro Tyr Ala Lys Ala Val Gly Glu Pro Tyr Lys100 105 110Leu Val Leu Thr Tyr Gln Gly Cys Ala Glu Ala Gly Val Cys Tyr Pro115 120 125Pro Val Asp Thr Glu Phe Asp Ile Phe Gly Asn Gly Thr Tyr His Pro130 135 140Gln Thr Asp Glu Pro Ala Ser Ala Lys Asp Arg Phe Leu Gln Pro Ser
145 150 155 160Ser Gln Asn Gly Ser Gly Ala Leu Pro Pro Pro Lys Gly Asp Glu Gly165 170 175Gly Asp Ser Arg Phe Lys Leu Ser Trp Asp Thr Leu Asn Ala Asn Leu180 185 190Leu Ala Phe Phe Leu Ala Gly Leu Gly Leu Ser Phe Thr Ala Cys Met195 200 205Tyr Pro Leu Leu Pro Ile Val Ser Ser Ile Val Val Gly Asp Lys Lys210 215 220Ala Gly Lys Ala Arg Ala Phe Val Leu Ser Val Val Tyr Val Gln Gly225 230 235 240Leu Ala Leu Thr Tyr Thr Leu Val Gly Ile Val Ala Gly Leu Thr Gly245 250 255Ala Leu Leu Thr Val Trp Leu Gln Gln Ala Trp Val Val Leu Ala Ala260 265 270Ser Ala Leu Met Val Val Leu Ala Leu Ser Met Phe Gly Leu Phe Asn275 280 285Ile Gln Leu Pro Asn Ala Val Gln Ser Tyr Phe Gln Asn Gln Ser Ser290 295 300Arg Leu Ser Gly Gly Lys Ile Val Ser Val Phe Ile Met Gly Ile Leu305 310 315 320Ser Ala Leu Ile Val Gly Pro Cys Val Ala Pro Pro Leu Ala Phe Ala325 330 335Leu Gly Tyr Ile Gly Gln Thr Gly Asp Ala Val Leu Gly Gly Leu Ala340 345 350Leu Tyr Thr Leu Ala Leu Gly Thr Gly Val Pro Leu Ile Ala Ile Gly355 360 365Thr Phe Gly Gly His Ile Leu Pro Lys Ala Gly Asp Trp Met Asn Ala370 375 380Val Lys Tyr Ala Phe Gly Phe Ile Leu Leu Ala Val Ala Val Tyr Leu385 390 395 400Ala Thr Pro His Leu Pro Tyr Tyr Leu Val Val Ala Leu Tyr Thr Leu
405 410 415Leu Met Leu Val Pro Ala Phe Met Leu Leu Val Asn Gly Arg Arg Gln420 425 430Lys Arg Arg Pro Lys Ala Val Ala Phe Ala Leu Gly Gly Ile Leu Leu435 440 445Ile Gly Gly Ala Trp Phe Gly Trp Gln Gly Ala Asn Gly Lys Thr Thr450 455 460Ala Leu His His Phe Leu Thr Leu Asn Pro Pro Ala Glu Ala Gly Lys465 470 475 480Ser Ser Glu His Gly Lys Met Phe Ala Asp Thr Ala Ala Leu Lys Ala485 490 495Ala Met Asp Thr Ala Leu Lys Glu His Pro Asp Lys Pro Val Val Leu500 505 510Asp Phe Tyr Ala Asp Trp Cys Ile Ser Cys Lys Glu Met Ala Ala Tyr515 520 525Thr Leu Asn Gln Pro Glu Val His Gln Ala Val Asp Met Glu Arg Phe530 535 540Phe Gln Ile Asp Val Thr Ala Asn Thr Pro Glu His Gln Ala Leu Leu545 550 555 560Lys Glu Tyr Gly Leu Phe Gly Pro Pro Gly Val Phe Val Val Arg Ser565 570 575Asp Gly Ser Arg Ser Glu Pro Leu Leu Gly Phe Val Lys Ala Asp Lys580 585 590Phe Ile Glu Trp Tyr Glu Gln Asn Arg595 600<210>13<211>1857<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(1857)
<400>13atg atg aac cgt tat cct tta tgg aaa tat ctg ctg att gtg ttc acg48Met Met Asn Arg Tyr Pro Leu Trp Lys Tyr Leu Leu Ile Val Phe Thr1 5 10 15att gcg gtt gcc gca gtg tat tcg ctg ccc aac cta ttc ggc gaa aca96Ile Ala Val Ala Ala Val Tyr Ser Leu Pro Asn Leu Phe Gly Glu Thr20 25 30ccc gcc gtg cag gta tcg acc aac cga caa gcc atc atc atc aac gaa144Pro Ala Val Gln Val Ser Thr Asn Arg Gln Ala Ile Ile Ile Asn Glu35 40 45cag act caa ttc aaa gtg gat gcc gcg ctg aaa aac gca ggt att cag192Gln Thr Gln Phe Lys Val Asp Ala Ala Leu Lys Asn Ala Gly Ile Gln50 55 60acc gac ggg atg ttt gtt gtg gac aat tca ctg aaa gtg cgt ttc aaa240Thr Asp Gly Met Phe Val Val Asp Asn Ser Leu Lys Val Arg Phe Lys65 70 75 80gac aca gaa acg cag ctt aaa gcg cgc gac gtc atc gaa aac act ttg288Asp Thr Glu Thr Gln Leu Lys Ala Arg Asp Val Ile Glu Asn Thr Leu85 90 95ggc gaa ggg tat att acc gcg ctc aac ctg ttg gcg gac agc ccc gaa336Gly Glu Gly Tyr Ile Thr Ala Leu Asn Leu Leu Ala Asp Ser Pro Glu100 105 110tgg atg gcg aaa atc aaa gcc aat ccg atg ttt ttg ggt ttg gac ctg384Trp Met Ala Lys Ile Lys Ala Asn Pro Met Phe Leu Gly Leu Asp Leu115 120 125cgc ggc ggc gtg cat ttc acc atg cag gtc gat atg aaa gcg gcg atg432Arg Gly Gly Val His Phe Thr Met Gln Val Asp Met Lys Ala Ala Met130 135 140cag aaa acg ttt gaa cgt tat tcg ggc gac atc cgc cgc gaa ctg cgc480Gln Lys Thr Phe Glu Arg Tyr Ser Gly Asp Ile Arg Arg Glu Leu Arg145 150 155 160cgc gaa aaa atc cgc agc ggc acg gtg cgt cag gct gga aac agc ctg528Arg Glu Lys Ile Arg Ser Gly Thr Val Arg Gln Ala Gly Asn Ser Leu165 170 175acc gtc cct ttg cag gat gca ggt gat gtg caa aag gct ctg ccg cag576Thr Val Pro Leu Gln Asp Ala Gly Asp Val Gln Lys Ala Leu Pro Gln
180 185 190ttg cgc aag ctg ttt cct gaa gca acg ctg aat tca gac ggc agc aat624Leu Arg Lys Leu Phe Pro Glu Ala Thr Leu Asn Ser Asp Gly Ser Asn195 200 205atc gtc ttg acg ctt tcg gaa gag gcg gtc aat aaa gtg tgt tcc gat672Ile Val Leu Thr Leu Ser Glu Glu Ala Val Asn Lys Val Cys Ser Asp210 215 220gcg gtc aaa cag aac atc act acc ctg cac aac cgt gtg aac gag ttg720Ala Val Lys Gln Asn Ile Thr Thr Leu His Asn Arg Val Asn Glu Leu225 230 235 240ggc gtg gcc gag ccc gtc atc cag cag tcc ggt gca gac cgt atc gtc768Gly Val Ala Glu Pro Val Ile Gln Gln Ser Gly Ala Asp Arg Ile Val245 250 255gtg cag ctt ccg ggc gtt cag gat act gcc aag gca aaa gac atc atc816Val Gln Leu Pro Gly Val Gln Asp Thr Ala Lys Ala Lys Asp Ile Ile260 265 270ggc cgt acc gcg act ttg gaa ttg cgt atg gtg gag gac gat cct gcc864Gly Arg Thr Ala Thr Leu Glu Leu Arg Met Val Glu Asp Asp Pro Ala275 280 285aag ttg cgc gag gca ttg gaa ggc aac gtg ccg agc ggt tat gag ctg912Lys Leu Arg Glu Ala Leu Glu Gly Asn Val Pro Ser Gly Tyr Glu Leu290 295 300ctt tca agc ggc gga gat cgt ccc gaa att ctg ctg atc agc aaa cag960Leu Ser Ser Gly Gly Asp Arg Pro Glu Ile Leu Leu Ile Ser Lys Gln305 310 315 320gtc gag ctg acg ggc gac aac atc aac gat gcg caa ccg agt ttc gac1008Val Glu Leu Thr Gly Asp Asn Ile Asn Asp Ala Gln Pro Ser Phe Asp325 330 335caa atg ggc gca cct gcc gtc agt ctg agc ttg gac agc gcg ggc ggc1056Gln Met Gly Ala Pro Ala Val Ser Leu Ser Leu Asp Ser Ala Gly Gly340 345 350agc att ttc ggc gaa ctg act gcc gca aat gtc ggc aaa cgc atg gcg1104Ser Ile Phe Gly Glu Leu Thr Ala Ala Asn Val Gly Lys Arg Met Ala355 360 365atg gtt ttg atc gac caa gga aaa tcc gag gtt gta acc gcg ccg gtt1152Met Val Leu Ile Asp Gln Gly Lys Ser Glu Val Val Thr Ala Pro Val
370 375 380atc cgt act gcc att acc ggc gga cgc gtg gaa att tcc gga agc atg1200Ile Arg Thr Ala Ile Thr Gly Gly Arg Val Glu Ile Ser Gly Ser Met385 390 395 400acg aca gcc gaa gcc aat gat acg tct ttg ctg ttg cgt gcc ggt tct1248Thr Thr Ala Glu Ala Asn Asp Thr Ser Leu Leu Leu Arg Ala Gly Ser405 410 415ctt gcc gca ccg atg cag att gtc gaa gaa cgt acc atc ggt ccg tct1296Leu Ala Ala Pro Met Gln Ile Val Glu Glu Arg Thr Ile Gly Pro Ser420 425 430ttg ggt aag gag aac atc gaa aaa ggc ttc cat tcg act tta tgg ggt1344Leu Gly Lys Glu Asn Ile Glu Lys Gly Phe His Ser Thr Leu Trp Gly435 440 445ttt gcc atc gtt gct gca ttc atg gtg gtt tac tat cgt ctg atg ggt1392Phe Ala Ile Val Ala Ala Phe Met Val Val Tyr Tyr Arg Leu Met Gly450 455 460ttc ttt tct acc att gca ttg agt gcc aac ata ctg ttc cta atc ggt1440Phe Phe Ser Thr Ile Ala Leu Ser Ala Asn Ile Leu Phe Leu Ile Gly465 470 475 480att ttg tct gcc atg cag gca acg ttg acg tta ccg ggt atg gcc gcg1488Ile Leu Ser Ala Met Gln Ala Thr Leu Thr Leu Pro Gly Met Ala Ala485 490 495ctg gcg ttg act ttg ggt atg gca atc gac tcc aac gtc ttg att aac1536Leu Ala Leu Thr Leu Gly Met Ala Ile Asp Ser Asn Val Leu Ile Asn500 505 510gaa cgt atc cgc gaa gaa ttg cgt gcc ggc gtg ccg ccg cag cag gca1584Glu Arg Ile Arg Glu Glu Leu Arg Ala Gly Val Pro Pro Gln Gln Ala515 520 525atc aat ctc ggt ttc caa cac gca tgg gcg acc att gtc gat tcg aac1632Ile Asn Leu Gly Phe Gln His Ala Trp Ala Thr Ile Val Asp Ser Asn530 535 540ctg act tcg ctg att gcc ggt atc gcg ctt ttg gta ttc ggt tcc ggc1680Leu Thr Ser Leu Ile Ala Gly Ile Ala Leu Leu Val Phe Gly Ser Gly545 550 555 560ccg gta cgc ggt ttt gcg gtc gta cac tgt ttg ggt att ctg act tcg1728Pro Val Arg Gly Phe Ala Val Val His Cys Leu Gly Ile Leu Thr Ser
565 570 575atg tat tca tcc gtc gtc gta ttc cgt gcg ttg gtc aat ctg tgg tac1776Met Tyr Ser Ser Val Val Val Phe Arg Ala Leu Val Asn Leu Trp Tyr580 585 590gga cgc aga cgc aaa ttg cag aat att tcc att ggt tcg gtg tgg aag1824Gly Arg Arg Arg Lys Leu Gln Asn Ile Ser Ile Gly Ser Val Trp Lys595 600 605ccg aaa gcc gaa atg gca gga ggc aag gag taa1857Pro Lys Ala Glu Met Ala Gly Gly Lys Glu610 615<210>14<211>618<212>PRT<213>脑膜炎奈瑟球菌<400>14Met Met Asn Arg Tyr Pro Leu Trp Lys Tyr Leu Leu Ile Val Phe Thr1 5 10 15Ile Ala Val Ala Ala Val Tyr Ser Leu Pro Asn Leu Phe Gly Glu Thr20 25 30Pro Ala Val Gln Val Ser Thr Asn Arg Gln Ala Ile Ile Ile Asn Glu35 40 45Gln Thr Gln Phe Lys Val Asp Ala Ala Leu Lys Asn Ala Gly Ile Gln50 55 60Thr Asp Gly Met Phe Val Val Asp Asn Ser Leu Lys Val Arg Phe Lys65 70 75 80Asp Thr Glu Thr Gln Leu Lys Ala Arg Asp Val Ile Glu Asn Thr Leu85 90 95Gly Glu Gly Tyr Ile Thr Ala Leu Asn Leu Leu Ala Asp Ser Pro Glu100 105 110Trp Met Ala Lys Ile Lys Ala Asn Pro Met Phe Leu Gly Leu Asp Leu115 120 125Arg Gly Gly Val His Phe Thr Met Gln Val Asp Met Lys Ala Ala Met130 135 140
Gln Lys Thr Phe Glu Arg Tyr Ser Gly Asp Ile Arg Arg Glu Leu Arg145 150 155 160Arg Glu Lys Ile Arg Ser Gly Thr Val Arg Gln Ala Gly Asn Ser Leu165 170 175Thr Val Pro Leu Gln Asp Ala Gly Asp Val Gln Lys Ala Leu Pro Gln180 185 190Leu Arg Lys Leu Phe Pro Glu Ala Thr Leu Asn Ser Asp Gly Ser Asn195 200 205Ile Val Leu Thr Leu Ser Glu Glu Ala Val Asn Lys Val Cys Ser Asp210 215 220Ala Val Lys Gln Asn Ile Thr Thr Leu His Asn Arg Val Asn Glu Leu225 230 235 240Gly Val Ala Glu Pro Val Ile Gln Gln Ser Gly Ala Asp Arg Ile Val245 250 255Val Gln Leu Pro Gly Val Gln Asp Thr Ala Lys Ala Lys Asp Ile Ile260 265 270Gly Arg Thr Ala Thr Leu Glu Leu Arg Met Val Glu Asp Asp Pro Ala275 280 285Lys Leu Arg Glu Ala Leu Glu Gly Asn Val Pro Ser Gly Tyr Glu Leu290 295 300Leu Ser Ser Gly Gly Asp Arg Pro Glu Ile Leu Leu Ile Ser Lys Gln305 310 315 320Val Glu Leu Thr Gly Asp Asn Ile Asn Asp Ala Gln Pro Ser Phe Asp325 330 335Gln Met Gly Ala Pro Ala Val Ser Leu Ser Leu Asp Ser Ala Gly Gly340 345 350Ser Ile Phe Gly Glu Leu Thr Ala Ala Asn Val Gly Lys Arg Met Ala355 360 365Met Val Leu Ile Asp Gln Gly Lys Ser Glu Val Val Thr Ala Pro Val370 375 380Ile Arg Thr Ala Ile Thr Gly Gly Arg Val Glu Ile Ser Gly Ser Met385 390 395 400
Thr Thr Ala Glu Ala Asn Asp Thr Ser Leu Leu Leu Arg Ala Gly Ser405 410 415Leu Ala Ala Pro Met Gln Ile Val Glu Glu Arg Thr Ile Gly Pro Ser420 425 430Leu Gly Lys Glu Asn Ile Glu Lys Gly Phe His Ser Thr Leu Trp Gly435 440 445Phe Ala Ile Val Ala Ala Phe Met Val Val Tyr Tyr Arg Leu Met Gly450 455 460Phe Phe Ser Thr Ile Ala Leu Ser Ala Asn Ile Leu Phe Leu Ile Gly465 470 475 480Ile Leu Ser Ala Met Gln Ala Thr Leu Thr Leu Pro Gly Met Ala Ala485 490 495Leu Ala Leu Thr Leu Gly Met Ala Ile Asp Ser Asn Val Leu Ile Asn500 505 510Glu Arg Ile Arg Glu Glu Leu Arg Ala Gly Val Pro Pro Gln Gln Ala515 520 525Ile Asn Leu Gly Phe Gln His Ala Trp Ala Thr Ile Val Asp Ser Asn530 535 540Leu Thr Ser Leu Ile Ala Gly Ile Ala Leu Leu Val Phe Gly Ser Gly545 550 555 560Pro Val Arg Gly Phe Ala Val Val His Cys Leu Gly Ile Leu Thr Ser565 570 575Met Tyr Ser Ser Val Val Val Phe Arg Ala Leu Val Asn Leu Trp Tyr580 585 590Gly Arg Arg Arg Lys Leu Gln Asn Ile Ser Ile Gly Ser Val Trp Lys595 600 605Pro Lys Ala Glu Met Ala Gly Gly Lys Glu610 615<210>15<211>1140<212>DNA<213>脑膜炎奈瑟球菌
<220>
<221>CDS<222>(1)..(1140)<400>15atg aaa aaa aca ctg gtg gcg gcg gca atc ctg agc ctc gcc ttg act48Met Lys Lys Thr Leu Val Ala Ala Ala Ile Leu Ser Leu Ala Leu Thr1 5 10 15gcg tgc ggc ggc gga agc gat acc gcc gcc caa acc ccc tcc gcc aag96Ala Cys Gly Gly Gly Ser Asp Thr Ala Ala Gln Thr Pro Ser Ala Lys20 25 30ccc gaa gcc gaa caa tcg ggc aaa ctc aac atc tac aac tgg tcg gat144Pro Glu Ala Glu Gln Ser Gly Lys Leu Asn Ile Tyr Asn Trp Ser Asp35 40 45tat gtc gat ccc gaa acc gtt gcc gcc ttt gaa aaa gaa acc ggc atc192Tyr Val Asp Pro Glu Thr Val Ala Ala Phe Glu Lys Glu Thr Gly Ile50 55 60aag acg cgt tcc gat tat tac gac agc aac gaa aca ctg gag gca aaa240Lys Thr Arg Ser Asp Tyr Tyr Asp Ser Asn Glu Thr Leu Glu Ala Lys65 70 75 80gtc ctg acc ggc aaa tcc ggc tac gac ctg acc gcg ccg tcc atc gcc288Val Leu Thr Gly Lys Ser Gly Tyr Asp Leu Thr Ala Pro Ser Ile Ala85 90 95aac gtc ggc cgg caa atc aaa gcg ggc gcg tat cag aaa atc gac aag336Asn Val Gly Arg Gln Ile Lys Ala Gly Ala Tyr Gln Lys Ile Asp Lys100 105 110gcg caa atc ccc cat tac ggc aac atc gat aaa gat ttg ctg aaa atg384Ala Gln Ile Pro His Tyr Gly Asn Ile Asp Lys Asp Leu Leu Lys Met115 120 125atg gaa gcc gtc gat ccg ggc aac gaa tac gcc gtc ccc tat ttc tgg432Met Glu Ala Val Asp Pro Gly Asn Glu Tyr Ala Val Pro Tyr Phe Trp130 135 140ggc atc aat acc ttg gca atc aat acc cag cag gtg aaa aaa gca ttg480Gly Ile Asn Thr Leu Ala Ile Asn Thr Gln Gln Val Lys Lys Ala Leu145 150 155 160ggt acg gac aag ctg ccc gaa aac gaa tgg gat ttg gtg ttc aaa ccc528Gly Thr Asp Lys Leu Pro Glu Asn Glu Trp Asp Leu Val Phe Lys Pro
165 170 175gaa tac acc gcc aaa ctc aaa tcc tgc ggc atc agc tat ttc gac agc576Glu Tyr Thr Ala Lys Leu Lys Ser Cys Gly Ile Ser Tyr Phe Asp Ser180 185 190gca atc gaa cag att ccc ttg gcg ttg cac tat ttg ggc aaa gac ccc624Ala Ile Glu Gln Ile Pro Leu Ala Leu His Tyr Leu Gly Lys Asp Pro195 200 205aac agt gag aat ccc gaa gac atc aaa gcc gcc gtc gat atg atg aaa672Asn Ser Glu Asn Pro Glu Asp Ile Lys Ala Ala Val Asp Met Met Lys210 215 220gcc gtc cgg ggc gac gtg aaa cgc ttc agc tct tcc ggc tat atc gac720Ala Val Arg Gly Asp Val Lys Arg Phe Ser Ser Ser Gly Tyr Ile Asp225 230 235 240gat atg gcg gcg ggc aac ctg tgt gcc gcc atc ggt tac ggc ggc gat768Asp Met Ala Ala Gly Asn Leu Cys Ala Ala Ile Gly Tyr Gly Gly Asp245 250 255ttg aac att gcc aaa acc cgt gcc gaa gaa gcc gca aac ggc gtg gaa816Leu Asn Ile Ala Lys Thr Arg Ala Glu Glu Ala Ala Asn Gly Val Glu260 265 270atc aaa gta ttg acc ccg aaa acc ggc gtg ggc gtg tgg gtg gat tcc864Ile Lys Val Leu Thr Pro Lys Thr Gly Val Gly Val Trp Val Asp Ser275 280 285ttt atg att ccg cgc gac gcg caa aac gtt gcc aat gcc cac cgc tat912Phe Met Ile Pro Arg Asp Ala Gln Asn Val Ala Asn Ala His Arg Tyr290 295 300atc gac tac acg ctc cgg ccc gag gtg gcg gcg aaa aac ggc agc ttc960Ile Asp Tyr Thr Leu Arg Pro Glu Val Ala Ala Lys Asn Gly Ser Phe305 310 315 320gtt acc tac gcg ccc gcc agc cgt ccc gcg cgc gag ctg atg gat gaa1008Val Thr Tyr Ala Pro Ala Ser Arg Pro Ala Arg Glu Leu Met Asp Glu325 330 335aaa tac acc tcc gac gca tcg att ttc ccg aac aaa gaa ctg atg gaa1056Lys Tyr Thr Ser Asp Ala Ser Ile Phe Pro Asn Lys Glu Leu Met Glu340 345 350aaa agt ttc atc gta tcg ccc aaa tcc gca gaa tcc gtc aaa ctg ggc1104Lys Ser Phe Ile Val Ser Pro Lys Ser Ala Glu Ser Val Lys Leu Gly
355 360 365gtg aag ctg tgg caa ggg ctc aaa gcg ggc aaa taa1140Val Lys Leu Trp Gln Gly Leu Lys Ala Gly Lys370 375 380<210>16<211>379<212>PRT<213>脑膜炎奈瑟球菌<400>16Met Lys Lys Thr Leu Val Ala Ala Ala Ile Leu Ser Leu Ala Leu Thr1 5 10 15Ala Cys Gly Gly Gly Ser Asp Thr Ala Ala Gln Thr Pro Ser Ala Lys20 25 30Pro Glu Ala Glu Gln Ser Gly Lys Leu Asn Ile Tyr Asn Trp Ser Asp35 40 45Tyr Val Asp Pro Glu Thr Val Ala Ala Phe Glu Lys Glu Thr Gly Ile50 55 60Lys Thr Arg Ser Asp Tyr Tyr Asp Ser Asn Glu Thr Leu Glu Ala Lys65 70 75 80Val Leu Thr Gly Lys Ser Gly Tyr Asp Leu Thr Ala Pro Ser Ile Ala85 90 95Asn Val Gly Arg Gln Ile Lys Ala Gly Ala Tyr Gln Lys Ile Asp Lys100 105 110Ala Gln Ile Pro His Tyr Gly Asn Ile Asp Lys Asp Leu Leu Lys Met115 120 125Met Glu Ala Val Asp Pro Gly Asn Glu Tyr Ala Val Pro Tyr Phe Trp130 135 140Gly Ile Asn Thr Leu Ala Ile Asn Thr Gln Gln Val Lys Lys Ala Leu145 150 155 160Gly Thr Asp Lys Leu Pro Glu Asn Glu Trp Asp Leu Val Phe Lys Pro165 170 175Glu Tyr Thr Ala Lys Leu Lys Ser Cys Gly Ile Ser Tyr Phe Asp Ser180 185 190
Ala Ile Glu Gln Ile Pro Leu Ala Leu His Tyr Leu Gly Lys Asp Pro195 200 205Asn Ser Glu Asn Pro Glu Asp Ile Lys Ala Ala Val Asp Met Met Lys210 215 220Ala Val Arg Gly Asp Val Lys Arg Phe Ser Ser Ser Gly Tyr Ile Asp225 230 235 240Asp Met Ala Ala Gly Asn Leu Cys Ala Ala Ile Gly Tyr Gly Gly Asp245 250 255Leu Asn Ile Ala Lys Thr Arg Ala Glu Glu Ala Ala Asn Gly Val Glu260 265 270Ile Lys Val Leu Thr Pro Lys Thr Gly Val Gly Val Trp Val Asp Ser275 280 285Phe Met Ile Pro Arg Asp Ala Gln Asn Val Ala Asn Ala His Arg Tyr290 295 300Ile Asp Tyr Thr Leu Arg Pro Glu Val Ala Ala Lys Asn Gly Ser Phe305 310 315 320Val Thr Tyr Ala Pro Ala Ser Arg Pro Ala Arg Glu Leu Met Asp Glu325 330 335Lys Tyr Thr Ser Asp Ala Ser Ile Phe Pro Asn Lys Glu Leu Met Glu340 345 350Lys Ser Phe Ile Val Ser Pro Lys Ser Ala Glu Ser Val Lys Leu Gly355 360 365Val Lys Leu Trp Gln Gly Leu Lys Ala Gly Lys370 375<210>17<211>453<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(453)
<400>17atg gca aaa gca atc gaa atc att tca ccc aat gaa att tat tca gat48Met Ala Lys Ala Ile Glu Ile Ile Ser Pro Asn Glu Ile Tyr Ser Asp1 5 10 15ctg att ttt aag gat ccg gta cct ccc cat act att gaa tat aaa atg96Leu Ile Phe Lys Asp Pro Val Pro Pro His Thr Ile Glu Tyr Lys Met20 25 30att aac cta gaa ttg aaa gcc atc aat ctt tac gat tgt gcc ttt gaa144Ile Asn Leu Glu Leu Lys Ala Ile Asn Leu Tyr Asp Cys Ala Phe Glu35 40 45gat ttc gtt ccc gaa atc aaa gac aat ttt tgt gtg gga att gat tta192Asp Phe Val Pro Glu Ile Lys Asp Asn Phe Cys Val Gly Ile Asp Leu50 55 60gac ata gga act gat gat agt gat gct gca gat ata ttt tct gtt cgc240Asp Ile Gly Thr Asp Asp Ser Asp Ala Ala Asp Ile Phe Ser Val Arg65 70 75 80ata tgc tct cct aaa tgg att ttg cat aat tgt ttc caa aat caa agg288Ile Cys Ser Pro Lys Trp Ile Leu His Asn Cys Phe Gln Asn Gln Arg85 90 95gtt aaa tgg ggg gcg ggt atg atg att atg aat gaa ttt aat cat tct336Val Lys Trp Gly Ala Gly Met Met Ile Met Asn Glu Phe Asn His Ser100 105 110gtt att aaa tcg gaa att gag aaa att ctt aaa gaa tgt tca aaa gaa384Val Ile Lys Ser Glu Ile Glu Lys Ile Leu Lys Glu Cys Ser Lys Glu115 120 125act tgg gaa aag tca ttg act tat tta ctt cgt ttt ttt tcg tgg gaa432Thr Trp Glu Lys Ser Leu Thr Tyr Leu Leu Arg Phe Phe Ser Trp Glu130 135 140ttt gaa gat tat cag tgc taa453Phe Glu Asp Tyr Gln Cys145 150<210>18<211>150<212>PRT<213>脑膜炎奈瑟球菌<400>18
Met Ala Lys Ala Ile Glu Ile Ile Ser Pro Asn Glu Ile Tyr Ser Asp1 5 10 15Leu Ile Phe Lys Asp Pro Val Pro Pro His Thr Ile Glu Tyr Lys Met20 25 30Ile Asn Leu Glu Leu Lys Ala Ile Asn Leu Tyr Asp Cys Ala Phe Glu35 40 45Asp Phe Val Pro Glu Ile Lys Asp Asn Phe Cys Val Gly Ile Asp Leu50 55 60Asp Ile Gly Thr Asp Asp Ser Asp Ala Ala Asp Ile Phe Ser Val Arg65 70 75 80Ile Cys Ser Pro Lys Trp Ile Leu His Asn Cys Phe Gln Asn Gln Arg85 90 95Val Lys Trp Gly Ala Gly Met Met Ile Met Asn Glu Phe Asn His Ser100 105 110Val Ile Lys Ser Glu Ile Glu Lys Ile Leu Lys Glu Cys Ser Lys Glu115 120 125Thr Trp Glu Lys Ser Leu Thr Tyr Leu Leu Arg Phe Phe Ser Trp Glu130 135 140Phe Glu Asp Tyr Gln Cys145 150<210>19<211>324<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(324)<400>19atg aaa aaa tgt att ttg ggc att ttg acc gcg tgt gcc gcc atg cct48Met Lys Lys Cys Ile Leu Gly Ile Leu Thr Ala Cys Ala Ala Met Pro1 5 10 15gca ttt gcc gac aga atc ggc gat ttg gaa gca cgt ctg gcg cag ttg96Ala Phe Ala Asp Arg Ile Gly Asp Leu Glu Ala Arg Leu Ala Gln Leu
20 25 30gaa cac cgt gtc gcc gta ttg gaa agc ggc ggc aat acc gtc aaa atc144Glu His Arg Val Ala Val Leu Glu Ser Gly Gly Asn Thr Val Lys Ile35 40 45gac ctt ttc ggt tca aat tcc acc atg tat gta tgc agc gtt acg cct192Asp Leu Phe Gly Ser Asn Ser Thr Met Tyr Val Cys Ser Val Thr Pro50 55 60ttt cag aag acg ttt gag gca agc gat cgg aat gaa ggc gtg gcg cgg240Phe Gln Lys Thr Phe Glu Ala Ser Asp Arg Asn Glu Gly Val Ala Arg65 70 75 80cag aaa gtg cgt cag gcg tgc aac cgc gaa act tcg gca atg ttt tgc288Gln Lys Val Arg Gln Ala Cys Asn Arg Glu Thr Ser Ala Met Phe Cys85 90 95gaa gat gag gca atc cga tgc aga aaa ttc gat tga324Glu Asp Glu Ala Ile Arg Cys Arg Lys Phe Asp100 105<210>20<211>107<212>PRT<213>脑膜炎奈瑟球菌<400>20Met Lys Lys Cys Ile Leu Gly Ile Leu Thr Ala Cys Ala Ala Met Pro1 5 10 15Ala Phe Ala Asp Arg Ile Gly Asp Leu Glu Ala Arg Leu Ala Gln Leu20 25 30Glu His Arg Val Ala Val Leu Glu Ser Gly Gly Asn Thr Val Lys Ile35 40 45Asp Leu Phe Gly Ser Asn Ser Thr Met Tyr Val Cys Ser Val Thr Pro50 55 60Phe Gln Lys Thr Phe Glu Ala Ser Asp Arg Asn Glu Gly Val Ala Arg65 70 75 80Gln Lys Val Arg Gln Ala Cys Asn Arg Glu Thr Ser Ala Met Phe Cys85 90 95Glu Asp Glu Ala Ile Arg Cys Arg Lys Phe Asp
100 105<210>21<211>519<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(519)<400>21atg gca aaa cat gaa att gaa gaa cgc ggt gac ggt ctg att gaa aag48Met Ala Lys His Glu Ile Glu Glu Arg Gly Asp Gly Leu Ile Glu Lys1 5 10 15atg gtc gct gtt aat cgc gta act aaa gta gtt aaa ggt ggc cgt atc96Met Val Ala Val Asn Arg Val Thr Lys Val Val Lys Gly Gly Arg Ile20 25 30atg gct ttc tca gca ctg act gtt gtt ggt gat ggt gat ggt cgc att144Met Ala Phe Ser Ala Leu Thr Val Val Gly Asp Gly Asp Gly Arg Ile35 40 45ggt atg ggc aaa ggt aaa tca aaa gaa gta cca gtt gct gtt caa aaa192Gly Met Gly Lys Gly Lys Ser Lys Glu Val Pro Val Ala Val Gln Lys50 55 60gca atg gat caa gct cga cgc tct atg att aaa gta cct ttg aaa aac240Ala Met Asp Gln Ala Arg Arg Ser Met Ile Lys Val Pro Leu Lys Asn65 70 75 80ggt act att cat cat gag gtt att ggc cgt cat ggt gct act aaa gta288Gly Thr Ile His His Glu Val Ile Gly Arg His Gly Ala Thr Lys Val85 90 95ttt atg cag cct gct aaa gag ggt agt ggc gta aaa gcc ggt gga cct336Phe Met Gln Pro Ala Lys Glu Gly Ser Gly Val Lys Ala Gly Gly Pro100 105 110atg cgt ttg gtt ttt gat gct atg ggc att cat aat atc tcc gcc aaa384Met Arg Leu Val Phe Asp Ala Met Gly Ile His Asn Ile Ser Ala Lys115 120 125gtg cac gga tct act aac cca tat aat atc gta cgt gca aca tta gat432Val His Gly Ser Thr Asn Pro Tyr Asn Ile Val Arg Ala Thr Leu Asp
130 135 140ggt ttg tct aag ttg cat act cct gct gat atc gca gcc aaa cgt ggc480Gly Leu Ser Lys Leu His Thr Pro Ala Asp Ile Ala Ala Lys Arg Gly145 150 155 160ttg aca gtg gaa gac att ttg gga gtt aac cat ggc tga519Leu Thr Val Glu Asp Ile Leu Gly Val Asn His Gly165 170<210>22<211>172<212>PRT<213>脑膜炎奈瑟球菌<400>22Met Ala Lys His Glu Ile Glu Glu Arg Gly Asp Gly Leu Ile Glu Lys1 5 10 15Met Val Ala Val Asn Arg Val Thr Lys Val Val Lys Gly Gly Arg Ile20 25 30Met Ala Phe Ser Ala Leu Thr Val Val Gly Asp Gly Asp Gly Arg Ile35 40 45Gly Met Gly Lys Gly Lys Ser Lys Glu Val Pro Val Ala Val Gln Lys50 55 60Ala Met Asp Gln Ala Arg Arg Ser Met Ile Lys Val Pro Leu Lys Asn65 70 75 80Gly Thr Ile His His Glu Val Ile Gly Arg His Gly Ala Thr Lys Val85 90 95Phe Met Gln Pro Ala Lys Glu Gly Ser Gly Val Lys Ala Gly Gly Pro100 105 110Met Arg Leu Val Phe Asp Ala Met Gly Ile His Asn Ile Ser Ala Lys115 120 125Val His Gly Ser Thr Asn Pro Tyr Asn Ile Val Arg Ala Thr Leu Asp130 135 140Gly Leu Ser Lys Leu His Thr Pro Ala Asp Ile Ala Ala Lys Arg Gly145 150 155 160Leu Thr Val Glu Asp Ile Leu Gly Val Asn His Gly
165 170<210>23<211>2028<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(2028)<400>23ttg tcc ctg tct gaa ttt ata gaa cgc cga acg tca ttt aat ccg atg48Leu Ser Leu Ser Glu Phe Ile Glu Arg Arg Thr Ser Phe Asn Pro Met1 5 10 15gtt att ttg acg act ttg ttt ttt gtg tgt gtt ttg gtg gta ttg gtt96Val Ile Leu Thr Thr Leu Phe Phe Val Cys Val Leu Val Val Leu Val20 25 30tta acc gtg ccg gat cag gtg cag atg tgg ctc gat cgg gca aaa gaa144Leu Thr Val Pro Asp Gln Val Gln Met Trp Leu Asp Arg Ala Lys Glu35 40 45gtc att ttt acc gag ttc agc tgg ttt tat gtt tta acg ttt tcc att192Val Ile Phe Thr Glu Phe Ser Trp Phe Tyr Val Leu Thr Phe Ser Ile50 55 60ttt ctg ggt ttc ctg ctg ata ctc tcg gtc agc agt ttg gga aac atc240Phe Leu Gly Phe Leu Leu Ile Leu Ser Val Ser Ser Leu Gly Asn Ile65 70 75 80agg ctc gga cgg gat gaa gat gtg ccg gaa ttc ggc ttc ctg tcg tgg288Arg Leu Gly Arg Asp Glu Asp Val Pro Glu Phe Gly Phe Leu Ser Trp85 90 95ctg gcg atg ctg ttt gcg gcc ggg atg ggc gtg ggt ctg atg ttt ttc336Leu Ala Met Leu Phe Ala Ala Gly Met Gly Val Gly Leu Met Phe Phe100 105 110ggc gtg gca gag ccg ttg atg cat tat ttt tcg gac att acg gcc ggc384Gly Val Ala Glu Pro Leu Met His Tyr Phe Ser Asp Ile Thr Ala Gly115 120 125acg ccg gaa cac agg cag cag cag gca ttg ctg cac acg gtg ttc cat432Thr Pro Glu His Arg Gln Gln Gln Ala Leu Leu His Thr Val Phe His
130 135 140tgg ggc gtt cac gct tgg tcg gtg tac ggt acg att gca ttg gct ttg480Trp Gly Val His Ala Trp Ser Val Tyr Gly Thr Ile Ala Leu Ala Leu145 150 155 160gct tat ttc ggt ttc cgc tac aag ctg ccg ctt gcc ctg cgt tct tgt528Ala Tyr Phe Gly Phe Arg Tyr Lys Leu Pro Leu Ala Leu Arg Ser Cys165 170 175ttt tac ccc ctg ttg aaa gaa aaa att tcc gga agg ttc ggc gat gcc576Phe Tyr Pro Leu Leu Lys Glu Lys Ile Ser Gly Arg Phe Gly Asp Ala180 185 190att gat att atg gcg ttg ctt gct act ttt ttc ggc atc atc acc aca624Ile Asp Ile Met Ala Leu Leu Ala Thr Phe Phe Gly Ile Ile Thr Thr195 200 205ttg ggg ttc ggg gct tcg caa ctg ggc gcc gga ttg cag gaa atg ggc672Leu Gly Phe Gly Ala Ser Gln Leu Gly Ala Gly Leu Gln Glu Met Gly210 215 220tgg att gcc gaa aac agc ttc agc gtg cag gtt ttg att atc gcc gcc720Trp Ile Ala Glu Asn Ser Phe Ser Val Gln Val Leu Ile Ile Ala Ala225 230 235 240gtc atg tcc ctc gcc gtc gtt tcg gca ata tcc ggc gtg ggg aag ggc768Val Met Ser Leu Ala Val Val Ser Ala Ile Ser Gly Val Gly Lys Gly245 250 255gtg aag gtg ttg agc gag ttg aac ctg ggc ctt gcg ttt ttg ctg ctg816Val Lys Val Leu Ser Glu Leu Asn Leu Gly Leu Ala Phe Leu Leu Leu260 265 270ttt ttt gtt ttg gcg gcg gga ccc act gtt tac ctg ttg tcg gca ttc864Phe Phe Val Leu Ala Ala Gly Pro Thr Val Tyr Leu Leu Ser Ala Phe275 280 285ggc gac aac ata ggg aac tac ctc gga aat ctg gtg cgc ctc agt ttt912Gly Asp Asn Ile Gly Asn Tyr Leu Gly Asn Leu Val Arg Leu Ser Phe290 295 300aaa act tat gcg tac gaa cgg gaa cac aag ccg tgg ttt gaa tct tgg960Lys Thr Tyr Ala Tyr Glu Arg Glu His Lys Pro Trp Phe Glu Ser Trp305 310 315 320acg gtg ctt tat tgg gcg tgg tgg tgt tct tgg gcg ccg ttt gtg ggt1008Thr Val Leu Tyr Trp Ala Trp Trp Cys Ser Trp Ala Pro Phe Val Gly
325 330 335ttg ttt atc gcg cgc att tca aag ggg cgc acc atc cgc gag ttt gtc1056Leu Phe Ile Ala Arg Ile Ser Lys Gly Arg Thr Ile Arg Glu Phe Val340 345 350ttc ggg gtt ttg ctc atc ccc ggc ctg ttc ggc gtt ttg tgg ttt acc1104Phe Gly Val Leu Leu Ile Pro Gly Leu Phe Gly Val Leu Trp Phe Thr355 360 365gtc ttc ggc aat acg gcg att tgg ctg aat gac ggg gtt gcg ggg gga1152Val Phe Gly Asn Thr Ala Ile Trp Leu Asn Asp Gly Val Ala Gly Gly370 375 380atg ctc gaa aag atg acc tcc tct ccg gaa acg ctg ctt ttt aaa ttc1200Met Leu Glu Lys Met Thr Ser Ser Pro Glu Thr Leu Leu Phe Lys Phe385 390 395 400ttt aat tac ctc ccc ctg ccc gaa ttg acg agc atc gtc agc ctg ctg1248Phe Asn Tyr Leu Pro Leu Pro Glu Leu Thr Ser Ile Val Ser Leu Leu405 410 415gtc att tct ctg ttt ttt gta act tct gcc gat tcc ggg att tat gtc1296Val Ile Ser Leu Phe Phe Val Thr Ser Ala Asp Ser Gly Ile Tyr Val420 425 430ctg aac aat att acc tct cgg gac aaa ggc ttg agc gcg cca cgg tgg1344Leu Asn Asn Ile Thr Ser Arg Asp Lys Gly Leu Ser Ala Pro Arg Trp435 440 445cag gcg gtt atg tgg ggc gtg ctg atg tct gcc gtt gcc gtt ttg ctg1392Gln Ala Val Met Trp Gly Val Leu Met Ser Ala Val Ala Val Leu Leu450 455 460atg cgc tcg ggc gga ctc ggc aac ctg cag tct atg acc ctg att gtt1440Met Arg Ser Gly Gly Leu Gly Asn Leu Gln Ser Met Thr Leu Ile Val465 470 475 480tcc ctg ccg ttt gcc ctg ctg atg ctg ata atg tgt ttc agc ctg tgg1488Ser Leu Pro Phe Ala Leu Leu Met Leu Ile Met Cys Phe Ser Leu Trp485 490 495aaa ggc ttg agt gcg gat aag aaa tat ttt gag acc cgg gtt aac cct1536Lys Gly Leu Ser Ala Asp Lys Lys Tyr Phe Glu Thr Arg Val Asn Pro500 505 510acc agt gta ttt tgg acg ggc ggc aag tgg aaa gaa cgg ctg gtg cag1584Thr Ser Val Phe Trp Thr Gly Gly Lys Trp Lys Glu Arg Leu Val Gln
515 520 525ata atg agc cag acg cag gag cag gat att tta aaa ttc ctc aaa cag1632Ile Met Ser Gln Thr Gln Glu Gln Asp Ile Leu Lys Phe Leu Lys Gln530 535 540act gca tcg ccc gct atg cac gag ttg caa cgg gag ctt tcg gaa gaa1680Thr Ala Ser Pro Ala Met His Glu Leu Gln Arg Glu Leu Ser Glu Glu545 550 555 560tac ggc ttg agc gtc cgg gtc gat aaa atg ttt cat cgg gac gag ccc1728Tyr Gly Leu Ser Val Arg Val Asp Lys Met Phe His Arg Asp Glu Pro565 570 575gca atc gag ttc gtc att cgg aaa gag acg atg cgc gat ttt atg tac1776Ala Ile Glu Phe Val Ile Arg Lys Glu Thr Met Arg Asp Phe Met Tyr580 585 590ggg att aag tct gtc ggg cag gat gta tcc gac cag ttg att aac gac1824Gly Ile Lys Ser Val Gly Gln Asp Val Ser Asp Gln Leu Ile Asn Asp595 600 605ggc aag ctg ccg cat atc cgg cat cag aca act tac aaa ccc tac gct1872Gly Lys Leu Pro His Ile Arg His Gln Thr Thr Tyr Lys Pro Tyr Ala610 615 620tat ttt ttc gac ggg cgc gtc ggg tac gat gtg cag tat atg aac aag1920Tyr Phe Phe Asp Gly Arg Val Gly Tyr Asp Val Gln Tyr Met Asn Lys625 630 635 640gac gag ctg att gcc gac att ttg aaa aac tac gaa cgt tat ttg atg1968Asp Glu Leu Ile Ala Asp Ile Leu Lys Asn Tyr Glu Arg Tyr Leu Met645 650 655ttg ttg gat gat gtc ggt cag gaa ctg atg gcg cac gag cag gtg gaa2016Leu Leu Asp Asp Val Gly Gln Glu Leu Met Ala His Glu Gln Val Glu660 665 670ttg gca gag taa2028Leu Ala Glu675<210>24<211>675<212>PRT<213>脑膜炎奈瑟球菌
<400>24Leu Ser Leu Ser Glu Phe Ile Glu Arg Arg Thr Ser Phe Asn Pro Met1 5 10 15Val Ile Leu Thr Thr Leu Phe Phe Val Cys Val Leu Val Val Leu Val20 25 30Leu Thr Val Pro Asp Gln Val Gln Met Trp Leu Asp Arg Ala Lys Glu35 40 45Val Ile Phe Thr Glu Phe Ser Trp Phe Tyr Val Leu Thr Phe Ser Ile50 55 60Phe Leu Gly Phe Leu Leu Ile Leu Ser Val Ser Ser Leu Gly Asn Ile65 70 75 80Arg Leu Gly Arg Asp Glu Asp Val Pro Glu Phe Gly Phe Leu Ser Trp85 90 95Leu Ala Met Leu Phe Ala Ala Gly Met Gly Val Gly Leu Met Phe Phe100 105 110Gly Val Ala Glu Pro Leu Met His Tyr Phe Ser Asp Ile Thr Ala Gly115 120 125Thr Pro Glu His Arg Gln Gln Gln Ala Leu Leu His Thr Val Phe His130 135 140Trp Gly Val His Ala Trp Ser Val Tyr Gly Thr Ile Ala Leu Ala Leu145 150 155 160Ala Tyr Phe Gly Phe Arg Tyr Lys Leu Pro Leu Ala Leu Arg Ser Cys165 170 175Phe Tyr Pro Leu Leu Lys Glu Lys Ile Ser Gly Arg Phe Gly Asp Ala180 185 190Ile Asp Ile Met Ala Leu Leu Ala Thr Phe Phe Gly Ile Ile Thr Thr195 200 205Leu Gly Phe Gly Ala Ser Gln Leu Gly Ala Gly Leu Gln Glu Met Gly210 215 220Trp Ile Ala Glu Asn Ser Phe Ser Val Gln Val Leu Ile Ile Ala Ala225 230 235 240Val Met Ser Leu Ala Val Val Ser Ala Ile Ser Gly Val Gly Lys Gly245 250 255
Val Lys Val Leu Ser Glu Leu Asn Leu Gly Leu Ala Phe Leu Leu Leu260 265 270Phe Phe Val Leu Ala Ala Gly Pro Thr Val Tyr Leu Leu Ser Ala Phe275 280 285Gly Asp Asn Ile Gly Asn Tyr Leu Gly Asn Leu Val Arg Leu Ser Phe290 295 300Lys Thr Tyr Ala Tyr Glu Arg Glu His Lys Pro Trp Phe Glu Ser Trp305 310 315 320Thr Val Leu Tyr Trp Ala Trp Trp Cys Ser Trp Ala Pro Phe Val Gly325 330 335Leu Phe Ile Ala Arg Ile Ser Lys Gly Arg Thr Ile Arg Glu Phe Val340 345 350Phe Gly Val Leu Leu Ile Pro Gly Leu Phe Gly Val Leu Trp Phe Thr355 360 365Val Phe Gly Asn Thr Ala Ile Trp Leu Asn Asp Gly Val Ala Gly Gly370 375 380Met Leu Glu Lys Met Thr Ser Ser Pro Glu Thr Leu Leu Phe Lys Phe385 390 395 400Phe Asn Tyr Leu Pro Leu Pro Glu Leu Thr Ser Ile Val Ser Leu Leu405 410 415Val Ile Ser Leu Phe Phe Val Thr Ser Ala Asp Ser Gly Ile Tyr Val420 425 430Leu Asn Asn Ile Thr Ser Arg Asp Lys Gly Leu Ser Ala Pro Arg Trp435 440 445Gln Ala Val Met Trp Gly Val Leu Met Ser Ala Val Ala Val Leu Leu450 455 460Met Arg Ser Gly Gly Leu Gly Asn Leu Gln Ser Met Thr Leu Ile Val465 470 475 480Ser Leu Pro Phe Ala Leu Leu Met Leu Ile Met Cys Phe Ser Leu Trp485 490 495Lys Gly Leu Ser Ala Asp Lys Lys Tyr Phe Glu Thr Arg Val Asn Pro500 505 510
Thr Ser Val Phe Trp Thr Gly Gly Lys Trp Lys Glu Arg Leu Val Gln515 520 525Ile Met Ser Gln Thr Gln Glu Gln Asp Ile Leu Lys Phe Leu Lys Gln530 535 540Thr Ala Ser Pro Ala Met His Glu Leu Gln Arg Glu Leu Ser Glu Glu545 550 555 560Tyr Gly Leu Ser Val Arg Val Asp Lys Met Phe His Arg Asp Glu Pro565 570 575Ala Ile Glu Phe Val Ile Arg Lys Glu Thr Met Arg Asp Phe Met Tyr580 585 590Gly Ile Lys Ser Val Gly Gln Asp Val Ser Asp Gln Leu Ile Asn Asp595 600 605Gly Lys Leu Pro His Ile Arg His Gln Thr Thr Tyr Lys Pro Tyr Ala610 615 620Tyr Phe Phe Asp Gly Arg Val Gly Tyr Asp Val Gln Tyr Met Asn Lys625 630 635 640Asp Glu Leu Ile Ala Asp Ile Leu Lys Asn Tyr Glu Arg Tyr Leu Met645 650 655Leu Leu Asp Asp Val Gly Gln Glu Leu Met Ala His Glu Gln Val Glu660 665 670Leu Ala Glu675<210>25<211>861<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(861)<400>25atg aaa ccc tat tcc gcc aat ccc aac ctg acc gaa ccg cgc cgg ctg48Met Lys Pro Tyr Ser Ala Asn Pro Asn Leu Thr Glu Pro Arg Arg Leu
1 5 10 15cgc gtg ttg ctg att gcc gcc gcg ctg ggc ttt ctg ctg ctg atg ctg96Arg Val Leu Leu Ile Ala Ala Ala Leu Gly Phe Leu Leu Leu Met Leu20 25 30gtc gtg ccg ctc gtc gcc gtg ttt tac gaa gcc tta aaa ggc ggt tgg144Val Val Pro Leu Val Ala Val Phe Tyr Glu Ala Leu Lys Gly Gly Trp35 40 45gat ttg tac ctg aaa tcc tta aac gat ccc gaa gcg tgg tct gcc atc192Asp Leu Tyr Leu Lys Ser Leu Asn Asp Pro Glu Ala Trp Ser Ala Ile50 55 60aaa ttg acg ctg att acc gcg ctg att gtc gtt ccc gtc aat gcc gta240Lys Leu Thr Leu Ile Thr Ala Leu Ile Val Val Pro Val Asn Ala Val65 70 75 80ttg ggt gtg gcg atg gcg tgg ctg ctg acc cgt ttt gat ttt cgc ggc288Leu Gly Val Ala Met Ala Trp Leu Leu Thr Arg Phe Asp Phe Arg Gly85 90 95aag cag ttg ctg acc acc ctg ctc gat ttg ccg ttt tcc gta tcg ccc336Lys Gln Leu Leu Thr Thr Leu Leu Asp Leu Pro Phe Ser Val Ser Pro100 105 110gtg gtg gcc ggt ttg atg ttc gtc tta ttg ttc ggc gcg cat acg gca384Val Val Ala Gly Leu Met Phe Val Leu Leu Phe Gly Ala His Thr Ala115 120 125ttg ggt ggc tgg ctc gaa gcg caa ggc ata cag att atc ttc gcc atc432Leu Gly Gly Trp Leu Glu Ala Gln Gly Ile Gln Ile Ile Phe Ala Ile130 135 140ccc ggt att gtt ttg gcg acg ctg ttc gtt acc ttc ccc ttt gtc gca480Pro Gly Ile Val Leu Ala Thr Leu Phe Val Thr Phe Pro Phe Val Ala145 150 155 160cgc gaa atc atc ccg ctg atg cag gca cag ggc gac agc gaa gaa cag528Arg Glu Ile Ile Pro Leu Met Gln Ala Gln Gly Asp Ser Glu Glu Gln165 170 175gcg gca ttg ata ctc ggc gca agc ggc tgg cag atg ttt tgg cgc gtt576Ala Ala Leu Ile Leu Gly Ala Ser Gly Trp Gln Met Phe Trp Arg Val180 185 190acc ctg ccc aac atc aaa tgg gcg tta ctc tac ggc atc atc ctc acc624Thr Leu Pro Asn Ile Lys Trp Ala Leu Leu Tyr Gly Ile Ile Leu Thr
195 200 205aac gcc cgc gcg atg ggc gag ttc ggc gcg gtc agc gtg gta tcg gga672Asn Ala Arg Ala Met Gly Glu Phe Gly Ala Val Ser Val Val Ser Gly210 215 220cac ata cgc ggc gaa acc aac acc gtc ccg ctt ttg gtc gaa atc ttc720His Ile Arg Gly Glu Thr Asn Thr Val Pro Leu Leu Val Glu Ile Phe225 230 235 240tac aac gaa tac aac ttc acc ggc gca ttc gcc ctc tcc ggc gta ttg768Tyr Asn Glu Tyr Asn Phe Thr Gly Ala Phe Ala Leu Ser Gly Val Leu245 250 255gca ctt ttg gca ctg gcg acg ctg gcg gtg cag aac atc att acc aaa816Ala Leu Leu Ala Leu Ala Thr Leu Ala Val Gln Asn Ile Ile Thr Lys260 265 270tta caa gac aaa aaa ctc gcc gcc gcc gaa agg aat gca ata tga861Leu Gln Asp Lys Lys Leu Ala Ala Ala Glu Arg Asn Ala Ile275 280 285<210>26<211>286<212>PRT<213>脑膜炎奈瑟球菌<400>26Met Lys Pro Tyr Ser Ala Asn Pro Asn Leu Thr Glu Pro Arg Arg Leu1 5 10 15Arg Val Leu Leu Ile Ala Ala Ala Leu Gly Phe Leu Leu Leu Met Leu20 25 30Val Val Pro Leu Val Ala Val Phe Tyr Glu Ala Leu Lys Gly Gly Trp35 40 45Asp Leu Tyr Leu Lys Ser Leu Asn Asp Pro Glu Ala Trp Ser Ala Ile50 55 60Lys Leu Thr Leu Ile Thr Ala Leu Ile Val Val Pro Val Asn Ala Val65 70 75 80Leu Gly Val Ala Met Ala Trp Leu Leu Thr Arg Phe Asp Phe Arg Gly85 90 95Lys Gln Leu Leu Thr Thr Leu Leu Asp Leu Pro Phe Ser Val Ser Pro
100 105 110Val Val Ala Gly Leu Met Phe Val Leu Leu Phe Gly Ala His Thr Ala115 120 125Leu Gly Gly Trp Leu Glu Ala Gln Gly Ile Gln Ile Ile Phe Ala Ile130 135 140Pro Gly Ile Val Leu Ala Thr Leu Phe Val Thr Phe Pro Phe Val Ala145 150 155 160Arg Glu Ile Ile Pro Leu Met Gln Ala Gln Gly Asp Ser Glu Glu Gln165 170 175Ala Ala Leu Ile Leu Gly Ala Ser Gly Trp Gln Met Phe Trp Arg Val180 185 190Thr Leu Pro Asn Ile Lys Trp Ala Leu Leu Tyr Gly Ile Ile Leu Thr195 200 205Asn Ala Arg Ala Met Gly Glu Phe Gly Ala Val Ser Val Val Ser Gly210 215 220His Ile Arg Gly Glu Thr Asn Thr Val Pro Leu Leu Val Glu Ile Phe225 230 235 240Tyr Asn Glu Tyr Asn Phe Thr Gly Ala Phe Ala Leu Ser Gly Val Leu245 250 255Ala Leu Leu Ala Leu Ala Thr Leu Ala Val Gln Asn Ile Ile Thr Lys260 265 270Leu Gln Asp Lys Lys Leu Ala Ala Ala Glu Arg Asn Ala Ile275 280 285<210>27<211>495<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(495)<400>27atg aaa aaa acc ctg ttg gca att gtt gcc gtt tcc gcc tta agt gcc48
Met Lys Lys Thr Leu Leu Ala Ile Val Ala Val Ser Ala Leu Ser Ala1 5 10 15tgc cgg cag gcg gaa gag gga ccg ccg cct tta ccc cgg cag att agc96cys Arg Gln Ala Glu Glu Gly Pro Pro Pro Leu Pro Arg Gln Ile Ser20 25 30gac cgt tcg gtc gga cac tat tgc agt atg aac ctg acc gaa cac aac144Asp Arg Ser Val Gly His Tyr Cys Ser Met Asn Leu Thr Glu His Asn35 40 45ggc ccc aaa gcc cag att ttc ttg aac ggc aaa ccc gat cag ccc gtt192Gly Pro Lys Ala Gln Ile Phe Leu Asn Gly Lys Pro Asp Gln Pro Val50 55 60tgg ttc tcc acc atc aag cag atg ttc ggc tat acc aag ctg ccc gaa240Trp Phe Ser Thr Ile Lys Gln Met Phe Gly Tyr Thr Lys Leu Pro Glu65 70 75 80gag cct aaa ggc atc cgc gtg att tac gtt acc gat atg ggc aat gtt288Glu Pro Lys Gly Ile Arg Val Ile Tyr Val Thr Asp Met Gly Asn Val85 90 95acc gat tgg acg aat ccc aat gcc gac acg gag tgg atg gat gcg aaa336Thr Asp Trp Thr Asn Pro Asn Ala Asp Thr Glu Trp Met Asp Ala Lys100 105 110aaa gcc ttt tac gtc atc gac agc ggc ttt atc ggc ggt atg ggt gcg384Lys Ala Phe Tyr Val Ile Asp Ser Gly Phe Ile Gly Gly Met Gly Ala115 120 125gaa gac gcg ctg ccg ttc ggc aac aaa gag cag gct gag aaa ttt gca432Glu Asp Ala Leu Pro Phe Gly Asn Lys Glu Gln Ala Glu Lys Phe Ala130 135 140aag gat aaa ggc ggt aag gtt gtc ggt ttc gac gat atg cct gat acc480Lys Asp Lys Gly Gly Lys Val Val Gly Phe Asp Asp Met Pro Asp Thr145 150 155 160tat att ttc aaa taa495Tyr Ile Phe Lys165<210>28<211>164<212>PRT<213>脑膜炎奈瑟球菌
<400>28Met Lys Lys Thr Leu Leu Ala Ile Val Ala Val Ser Ala Leu Ser Ala1 5 10 15Cys Arg Gln Ala Glu Glu Gly Pro Pro Pro Leu Pro Arg Gln Ile Ser20 25 30Asp Arg Ser Val Gly His Tyr Cys Ser Met Asn Leu Thr Glu His Asn35 40 45Gly Pro Lys Ala Gln Ile Phe Leu Asn Gly Lys Pro Asp Gln Pro Val50 55 60Trp Phe Ser Thr Ile Lys Gln Met Phe Gly Tyr Thr Lys Leu Pro Glu65 70 75 80Glu Pro Lys Gly Ile Arg Val Ile Tyr Val Thr Asp Met Gly Asn Val85 90 95Thr Asp Trp Thr Asn Pro Asn Ala Asp Thr Glu Trp Met Asp Ala Lys100 105 110Lys Ala Phe Tyr Val Ile Asp Ser Gly Phe Ile Gly Gly Met Gly Ala115 120 125Glu Asp Ala Leu Pro Phe Gly Asn Lys Glu Gln Ala Glu Lys Phe Ala130 135 140Lys Asp Lys Gly Gly Lys Val Val Gly Phe Asp Asp Met Pro Asp Thr145 150 155 160Tyr Ile Phe Lys<210>29<211>810<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(810)<400>29atg aga cca tat gct act act att tat caa ctt ttt att ttg ttt att48Met Arg Pro Tyr Ala Thr Thr Ile Tyr Gln Leu Phe Ile Leu Phe Ile
1 5 10 15ggg agt gtt ttt act atg acc tca tgt gaa cct gtg aat gaa aag aca96Gly Ser Val Phe Thr Met Thr Ser Cys Glu Pro Val Asn Glu Lys Thr20 25 30gat caa aaa gca gta agt gcg caa cag gct aaa gaa caa acc agt ttc144Asp Gln Lys Ala Val Ser Ala Gln Gln Ala Lys Glu Gln Thr Ser Phe35 40 45aac aat ccc gag cca atg aca gga ttt gaa cat acg gtt aca ttt gat192Asn Asn Pro Glu Pro Met Thr Gly Phe Glu His Thr Val Thr Phe Asp50 55 60ttt cag ggc acc aaa atg gtt atc ccc tat ggc tat ctt gca cgg tat240Phe Gln Gly Thr Lys Met Val Ile Pro Tyr Gly Tyr Leu Ala Arg Tyr65 70 75 80acg caa gac aat gcc aca aaa tgg ctt tcc gac acg cca ggg cag gat288Thr Gln Asp Asn Ala Thr Lys Trp Leu Ser Asp Thr Pro Gly Gln Asp85 90 95gct tac tcc att aat ttg ata gag att agc gtc tat tac aaa aaa acc336Ala Tyr Ser Ile Asn Leu Ile Glu Ile Ser Val Tyr Tyr Lys Lys Thr100 105 110gac caa ggc tgg gtg ctc gaa cca tac aac cag caa aac aaa gcg cac384Asp Gln Gly Trp Val Leu Glu Pro Tyr Asn Gln Gln Asn Lys Ala His115 120 125ttt atc caa ttt cta cgc gac ggt ttg gat agc gtg gac gat att gtt432Phe Ile Gln Phe Leu Arg Asp Gly Leu Asp Ser Val Asp Asp Ile Val130 135 140atc cga aaa gat gcg tgt agt tta agc acg act atg gga gaa aga ttg480Ile Arg Lys Asp Ala Cys Ser Leu Ser Thr Thr Met Gly Glu Arg Leu145 150 155 160ctt act tac ggg gtt aaa aaa atg cca tct gcc tat cct gaa tac gag528Leu Thr Tyr Gly Val Lys Lys Met Pro Ser Ala Tyr Pro Glu Tyr Glu165 170 175gct tat gaa gat aaa aga cat att cct gaa aat cca tat ttt cat gaa576Ala Tyr Glu Asp Lys Arg His Ile Pro Glu Asn Pro Tyr Phe His Glu180 185 190ttt tac tat att aaa aaa gga gaa aat ccg gcg att att act cat cgg624Phe Tyr Tyr Ile Lys Lys Gly Glu Asn Pro Ala Ile Ile Thr His Arg
195 200 205aac tat cat agg tat gga gag aac gat tac agc act agc gta ggt tcc672Asn Tyr His Arg Tyr Gly Glu Asn Asp Tyr Ser Thr Ser Val Gly Ser210 215 220tgt att aac ggt ttc acg gta cgg tat tac ccg ttt att cgg gaa aag720Cys Ile Asn Gly Phe Thr Val Arg Tyr Tyr Pro Phe Ile Arg Glu Lys225 230 235 240cag cag ctc aca cag cag gag ttg gta ggt tat cac caa caa gta gag768Gln Gln Leu Thr Gln Gln Glu Leu Val Gly Tyr His Gln Gln Val Glu245 250 255caa ttg gta cag agt ttt gta aac aat cca agt aaa aaa taa810Gln Leu Val Gln Ser Phe Val Asn Asn Pro Ser Lys Lys260 265 270<210>30<211>269<212>PRT<213>脑膜炎奈瑟球菌<400>30Met Arg Pro Tyr Ala Thr Thr Ile Tyr Gln Leu Phe Ile Leu Phe Ile1 5 10 15Gly Ser Val Phe Thr Met Thr Ser Cys Glu Pro Val Asn Glu Lys Thr20 25 30Asp Gln Lys Ala Val Ser Ala Gln Gln Ala Lys Glu Gln Thr Ser Phe35 40 45Asn Asn Pro Glu Pro Met Thr Gly Phe Glu His Thr Val Thr Phe Asp50 55 60Phe Gln Gly Thr Lys Met Val Ile Pro Tyr Gly Tyr Leu Ala Arg Tyr65 70 75 80Thr Gln Asp Asn Ala Thr Lys Trp Leu Ser Asp Thr Pro Gly Gln Asp85 90 95Ala Tyr Ser Ile Asn Leu Ile Glu Ile Ser Val Tyr Tyr Lys Lys Thr100 105 110Asp Gln Gly Trp Val Leu Glu Pro Tyr Asn Gln Gln Asn Lys Ala His115 120 125
Phe Ile Gln Phe Leu Arg Asp Gly Leu Asp Ser Val Asp Asp Ile Val130 135 140Ile Arg Lys Asp Ala Cys Ser Leu Ser Thr Thr Met Gly Glu Arg Leu145 150 155 160Leu Thr Tyr Gly Val Lys Lys Met Pro Ser Ala Tyr Pro Glu Tyr Glu165 170 175Ala Tyr Glu Asp Lys Arg His Ile Pro Glu Asn Pro Tyr Phe His Glu180 185 190Phe Tyr Tyr Ile Lys Lys Gly Glu Asn Pro Ala Ile Ile Thr His Arg195 200 205Asn Tyr His Arg Tyr Gly Glu Asn Asp Tyr Ser Thr Ser Val Gly Ser210 215 220Cys Ile Asn Gly Phe Thr Val Arg Tyr Tyr Pro Phe Ile Arg Glu Lys225 230 235 240Gln Gln Leu Thr Gln Gln Glu Leu Val Gly Tyr His Gln Gln Val Glu245 250 255Gln Leu Val Gln Ser Phe Val Asn Asn Pro Ser Lys Lys260 265<210>31<211>285<212>DNA<213>脑膜炎奈瑟球菌<220>
<221>CDS<222>(1)..(285)<400>31atg aag caa gtc aag aag tcg tct tat ttt aaa tat caa aaa agg aaa48Met Lys Gln Val Lys Lys Ser Ser Tyr Phe Lys Tyr Gln Lys Arg Lys1 5 10 15aaa acg atg aac atc gtt aaa aaa tac gct gta aaa gca gcc ttg gca96Lys Thr Met Asn Ile Val Lys Lys Tyr Ala Val Lys Ala Ala Leu Ala20 25 30
gcc ggt atc ttc aca ccg gcc att gtt atg gca gat acc ttt gat cca144Ala Gly Ile Phe Thr Pro Ala Ile Val Met Ala Asp Thr Phe Asp Pro35 40 45tcc gcg att ggt acg caa gta gcg aat gta atc atg ggt ttc gtg tca192Ser Ala Ile Gly Thr Gln Val Ala Asn Val Ile Met Gly Phe Val Ser50 55 60atg gtt tcc gcc gtg ggt atg gcg gcc att acc gtg att ctt gca atc240Met Val Ser Ala Val Gly Met Ala Ala Ile Thr Val Ile Leu Ala Ile65 70 75 80caa ggc ttc aaa atg gct tgg agc atg att aaa tct gtc aaa taa285Gln Gly Phe Lys Met Ala Trp Ser Met Ile Lys Ser Val Lys85 90 95<210>32<211>94<212>PRT<213>脑膜炎奈瑟球菌<400>32Met Lys Gln Val Lys Lys Ser Ser Tyr Phe Lys Tyr Gln Lys Arg Lys1 5 10 15Lys Thr Met Asn Ile Val Lys Lys Tyr Ala Val Lys Ala Ala Leu Ala20 25 30Ala Gly Ile Phe Thr Pro Ala Ile Val Met Ala Asp Thr Phe Asp Pro35 40 45Ser Ala Ile Gly Thr Gln Val Ala Asn Val Ile Met Gly Phe Val Ser50 55 60Met Val Ser Ala Val Gly Met Ala Ala Ile Thr Val Ile Leu Ala Ile65 70 75 80Gln Gly Phe Lys Met Ala Trp Ser Met Ile Lys Ser Val Lys85 90<210>33<211>966<212>DNA<213>脑膜炎奈瑟球菌
<220>
<221>CDS<222>(1)..(966)<400>33gtg aaa ccg cgt ttt tat tgg gca gcc tgc gcc gtc ctg ctg acc gcc48Val Lys Pro Arg Phe Tyr Trp Ala Ala Cys Ala Val Leu Leu Thr Ala1 5 10 15tgt tcg ccc gaa cct gcc gcc gaa aaa act gta tcc gcc gca tcc gca96Cys Ser Pro Glu Pro Ala Ala Glu Lys Thr Val Ser Ala Ala Ser Ala20 25 30tct gcc gcc acg ctg acc gtg ccg acc gcg cgg ggc gat gcc gtt gtg144Ser Ala Ala Thr Leu Thr Val Pro Thr Ala Arg Gly Asp Ala Val Val35 40 45ccg aag aat ccc gaa cgc gtc gcc gtg tac gac tgg gcg gcg ttg gat192Pro Lys Asn Pro Glu Arg Val Ala Val Tyr Asp Trp Ala Ala Leu Asp50 55 60acg ctg acc gaa ttg ggc gtg aat gtg ggc gca acc acc gcg ccg gtg240Thr Leu Thr Glu Leu Gly Val Asn Val Gly Ala Thr Thr Ala Pro Val65 70 75 80cgc gtg gat tat ttg cag cct gca ttt gac aag gcg gca acg gtg ggg288Arg Val Asp Tyr Leu Gln Pro Ala Phe Asp Lys Ala Ala Thr Val Gly85 90 95acg ctg ttc gag ccc gat tac gaa gcc ctg cac cgc tac aat cct cag336Thr Leu Phe Glu Pro Asp Tyr Glu Ala Leu His Arg Tyr Asn Pro Gln100 105 110ctt gtc att acc ggc ggg ccg ggc gcg gaa gcg tat gaa cag tta gcg384Leu Val Ile Thr Gly Gly Pro Gly Ala Glu Ala Tyr Glu Gln Leu Ala115 120 125aaa aac gcg acc acc ata gat ctg acg gtg gac aac ggc aat atc cgc432Lys Asn Ala Thr Thr Ile Asp Leu Thr Val Asp Asn Gly Asn Ile Arg130 135 140acc agc ggc gaa aag cag atg gag acc ttg gcg cgg att ttc ggc aag480Thr Ser Gly Glu Lys Gln Met Glu Thr Leu Ala Arg Ile Phe Gly Lys145 150 155 160gaa gcg cgc gcg gcg gaa ttg aag gcg cag att gac gcg ctg ttc gcc528Glu Ala Arg Ala Ala Glu Leu Lys Ala Gln Ile Asp Ala Leu Phe Ala165 170 175
caa acg cgc gaa gcc gcc aaa ggc aaa gga cgc ggg ctg gtg ctg tcg576Gln Thr Arg Glu Ala Ala Lys Gly Lys Gly Arg Gly Leu Val Leu Ser180 185 190gtt acg ggc aac aag gtg tcc gcc ttc ggc acg cag tcg cgg ttg gca624Val Thr Gly Asn Lys Val Ser Ala Phe Gly Thr Gln Ser Arg Leu Ala195 200 205agt tgg ata cac ggc gac atc ggc cta ccg cct gta gac gaa tct tta672Ser Trp Ile His Gly Asp Ile Gly Leu Pro Pro Val Asp Glu Ser Leu210 215 220cgc aac gag ggg cac ggg cag cct gtt tcc ttc gaa tac atc aaa gag720Arg Asn Glu Gly His Gly Gln Pro Val Ser Phe Glu Tyr Ile Lys Glu225 230 235 240aaa aac ccc gat tgg att ttc atc atc gac cgt acc gcc gcc atc ggg768Lys Asn Pro Asp Trp Ile Phe Ile Ile Asp Arg Thr Ala Ala Ile Gly245 250 255cag gaa ggg ccg gcg gct gtc gaa gta ttg gat aac gcg ctg gta cgc816Gln Glu Gly Pro Ala Ala Val Glu Val Leu Asp Asn Ala Leu Val Arg260 265 270ggc acg aac gct tgg aag cgc aag caa atc atc gtc atg cct gcc gcg864Gly Thr Asn Ala Trp Lys Arg Lys Gln Ile Ile Val Met Pro Ala Ala275 280 285aac tac att gtc gcg ggc ggc gcg cgg cag ttg att cag gcg gcg gag912Asn Tyr Ile Val Ala Gly Gly Ala Arg Gln Leu Ile Gln Ala Ala Glu290 295 300cag ttg aag gcg gcg ttt aaa aag gca gaa ccc gtt gcg gcg ggg aaa960Gln Leu Lys Ala Ala Phe Lys Lys Ala Glu Pro Val Ala Ala Gly Lys305 310 315 320aag tag966Lys<210>34<211>321<212>PRT<213>脑膜炎奈瑟球菌<400>34Val Lys Pro Arg Phe Tyr Trp Ala Ala Cys Ala Val Leu Leu Thr Ala
1 5 10 15Cys Ser Pro Glu Pro Ala Ala Glu Lys Thr Val Ser Ala Ala Ser Ala20 25 30Ser Ala Ala Thr Leu Thr Val Pro Thr Ala Arg Gly Asp Ala Val Val35 40 45Pro Lys Asn Pro Glu Arg Val Ala Val Tyr Asp Trp Ala Ala Leu Asp50 55 60Thr Leu Thr Glu Leu Gly Val Asn Val Gly Ala Thr Thr Ala Pro Val65 70 75 80Arg Val Asp Tyr Leu Gln Pro Ala Phe Asp Lys Ala Ala Thr Val Gly85 90 95Thr Leu Phe Glu Pro Asp Tyr Glu Ala Leu His Arg Tyr Asn Pro Gln100 105 110Leu Val Ile Thr Gly Gly Pro Gly Ala Glu Ala Tyr Glu Gln Leu Ala115 120 125Lys Asn Ala Thr Thr Ile Asp Leu Thr Val Asp Asn Gly Asn Ile Arg130 135 140Thr Ser Gly Glu Lys Gln Met Glu Thr Leu Ala Arg Ile Phe Gly Lys145 150 155 160Glu Ala Arg Ala Ala Glu Leu Lys Ala Gln Ile Asp Ala Leu Phe Ala165 170 175Gln Thr Arg Glu Ala Ala Lys Gly Lys Gly Arg Gly Leu Val Leu Ser180 185 190Val Thr Gly Asn Lys Val Ser Ala Phe Gly Thr Gln Ser Arg Leu Ala195 200 205Ser Trp Ile His Gly Asp Ile Gly Leu Pro Pro Val Asp Glu Ser Leu210 215 220Arg Asn Glu Gly His Gly Gln Pro Val Ser Phe Glu Tyr Ile Lys Glu225 230 235 240Lys Asn Pro Asp Trp Ile Phe Ile Ile Asp Arg Thr Ala Ala Ile Gly245 250 255Gln Glu Gly Pro Ala Ala Val Glu Val Leu Asp Asn Ala Leu Val Arg
260 265 270Gly Thr Asn Ala Trp Lys Arg Lys Gln Ile Ile Val Met Pro Ala Ala275 280 285Asn Tyr Ile Val Ala Gly Gly Ala Arg Gln Leu Ile Gln Ala Ala Glu290 295 300Gln Leu Lys Ala Ala Phe Lys Lys Ala Glu Pro Val Ala Ala Gly Lys305 310 315 320Lys<210>35<211>24<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>35atgcgaaaaa aacttaccgc cctc 24<210>36<211>18<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>36gaatttgtgg cgcaaacc18<210>37<211>24<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸
<400>37gaggaagaaa atcattgccg cgac24<210>38<211>20<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>38atggtgtccg tattcgccgc 20<210>39<211>24<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>39atggcttttt atgcttttaa ggcg 24<210>40<211>24<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>40tttcgcttca gaagcaggtt tggc 24<210>41<211>21<212>DNA
<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>41tttgcccgct ttgagccctt g21<210>42<211>24<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>42atgctcaaca tctacaactg gtcg24<210>43<211>21<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>43ggagtcggca aaaaggtggg c21<210>44<211>22<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>44atgctctcct cacagtctgc cc 22
<210>45<211>25<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>45atggaactct ttaaaatcaa acgcg25<210>46<211>25<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>46aaccacgatt tcttctttct tcttc25<210>47<211>20<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸<400>47atgagaccat atgctactac 20<210>48<211>21<212>DNA<213>人工序列<220>
<223>人工序列描述合成的寡核苷酸
<400>48ttttttactt ggattgttta c2权利要求
1.一种肽，由包括脑膜炎奈瑟球菌的、SEQ ID NOS.1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、和33此处确定的任一种核苷酸序列的基因或其革兰氏阴性细菌同系物编码，或其功能性片段，用于治疗或诊断用途。
2.权利要求1的肽，其中所述的同系物在肽和核苷酸水平上有至少40％的序列相似性和同一性。
3.权利要求1的或权利要求2的肽，其中所述的同系物在肽和核苷酸水平上有至少60％的序列相似性和同一性。
4.任一前述权利要求的肽，其中所述的同系物在肽和核苷酸水平上有至少90％的序列相似性和同一性。
5.权利要求1的肽，包含SEQ ID NOS.2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32和34此处定义的任一氨基酸序列。
6.编码任一前述权利要求的肽的多核苷酸，用于治疗或诊断用途。
7.表达权利要求1-5任一项中的肽的转化宿主。
8.包含突变的微生物，该突变破坏权利要求1定义的任一核苷酸序列的表达。
9.权利要求8的微生物，其中所述的突变是插入失活或基因缺失。
10.权利要求8或权利要求9的微生物，其中所述的微生物是脑膜炎奈瑟球菌。
11.权利要求8-10任一项中的微生物，包含在第二核苷酸序列中的突变。
12.权利要求8-11任一项中的微生物，用于治疗或诊断用途。
13.一种疫苗，含有权利要求1-5任一项中的肽或其表达方法。
14.针对权利要求1-5任一项中的肽的抗体。
15.权利要求1-7任一项的产物在筛选分析中的用途。
16.权利要求1-12任一项的产物的用途，用于制备用于治疗或预防奈瑟球菌属或革兰氏阴性细菌感染相关的疾病的药物。
全文摘要
本发明公开了来自脑膜炎奈瑟球菌(Neisseriameningitides)、编码用于免疫靶物质的一系列基因。由此鉴定的这些基因可以制备疫苗和其他治疗产物。
文档编号G01N33/68GK1545552SQ01815395
公开日2004年11月10日 申请日期2001年8月21日 优先权日2000年8月24日
发明者J·D·雷恩, M·J·G·胡格斯, J·D·圣汤戈罗, G 胡格斯, J D 雷恩, 圣汤戈罗 申请人:微科学有限公司