包含枯草杆菌蛋白酶变体的组合物和方法

专利名称：包含枯草杆菌蛋白酶变体的组合物和方法
技术领域：
本发明提供丝氨酸蛋白酶变体。具体地讲，本发明提供与参照丝氨酸蛋白酶相比具有一个或多个置换的丝氨酸蛋白酶变体。此外，本发明提供包含这些丝氨酸蛋白酶变体 的组合物。在一些实施例中，本发明提供清洁组合物，其包含这些丝氨酸蛋白酶变体中的至少一种。
背景技术：
尽管丝氨酸蛋白酶在工业酶领域中已久为人所知，但对于适于特定条件和用途的工程化蛋白酶仍存在需要。

发明内容
本发明提供丝氨酸蛋白酶变体。具体地讲，本发明提供与参照丝氨酸蛋白酶相比具有一个或多个置换的丝氨酸蛋白酶变体。此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。在一些实施例中，本发明提供清洁组合物，其包含这些丝氨酸蛋白酶变体中的至少一种。本发明提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X1R，X2W, X2M, X2R, X2A, X2S, X3R, X4R, X4C, X4S, X8A, X9F, X9W, X9A, X10S, X10M, X10H, X10A, X12R, X12F,X14K, X14F, X14Q, X15R, X15F, X16S, X17R, X17M, X17F, X18R, X18K, X20F, X20R, X20K, X22Y, X22A, X22R, X22V, X22Q, X22W, X22L, X23F, X23S, X23A, X24W, X24R, X24H, X24F, X24Q, X24L, X25V，X25F, X25R, X26F, X27V, X27F, X27L, X27R, X28N, X28E, X28A, X29T, X30E, X31F, X33D, X33G, X33S, X34P, X35M, X36F, X36R, X36T, X38L, X38F, X38R, X40L, X40W, X40N, X40R, X40T, X40H, X42I, X43D, X43I, X43R, X43M, X43F, X43W, X43S, X43A, X45T, X46R, X48R, X50C, X51H, X51W, X51F,X52F, X52E, X52N, X55Y, X57R, X59R, X59A, X59F, X60A, X60Q, X60P, X62E, X62Q, X63I, X63V, X63T, X63P, X63D, X63M, X63H, X63Q, X63E, X63A, X63S, X64F, X64T, X68C, X68A, X69N, X69P, X69ff, X69T, X71G, X72C, X74C, X75F, X75A, X75R, X75E, X76D, X78I, X78R, X78N, X79W, X79Q, X81R，X82V, X82T, X82F, X82M, X82R, X85M, X86L, X86I, X86W, X89P, X89T, X89V, X89G, X89W, X89H,X89F, X89L, X89I, X91N, X91F, X92F, X94N, X99G, X99F, X99M, X99T, X99P, X100I, X100S, XlOON, X100Q, X101N, X101A, X101G, X101P, X101F, X101E, X101T, X101D, X102H, X102N, X102E, Xl02T, X102A, X103G, X103D, X103N, X104D, X104E, X104I,X104L, X105Q, X105E, X105T, X106F,X106V, X106G, X106E, X106T, X106D, X106A, X107F, X107M, X108G, X108I, X109M, X111V, XlllI, X112V, X112L, X112Q, X114G, X115K, X115R, X116A, X116K, X116L, X117F, X118I, X118R, Xl19C, X120F, X120A, X120R, X121E, X121F, X123G, X123E, X124S, X128F, X128H, X128I, X128L,X128Q, X128N, X128M, X128D, X129E, X132E, X132A, X138G, X144R, X147L, X148I,X158E,X158E, X159E, X159C, X160D, X166D, X166E, X167W, X175V, X177C, X181A, X182R, X183F, X183I,X183D, X183R, X183M, X185E, X185I, X185V, X186H, X186K, X188R, X188E, X188D, X192H, X192ff, X194V, X194F, X194E, X197F, X198L, X198F, X203E, X203C, X208S, X209N, X209F, X209E,X209S, X209H, X209G, X209T, X209L, X210R, X210V, X210L, X211R, X211Q, X212I, X212M, X212F,X213A, X214F, X215F, X215N, X215D, X215H, X215E, X216F, X216A, X217N, X217E, X217D, X218P,X218D, X218E, X224A, X224G, X227I, X230E, X231I, X231C, X233C, X234F, X235F, X236F, X236N, X238L, X238K, X238R, X239K, X239S, X239T, X239G, X239H, X239R, X239N, X239F, X240R,X241R, X242L, X242R, X243R, X243F, X244R, X246S, X248I, X248V, X248R, X249R, X249T, X250I, X251S, X251R, X252I, X252F, X252H, X252R, X253F, X253I, X253R, X254C, X256N, X258R, X260V, X260I, X262H, X262D, X263F, X265F, X267N, X267V, X267M, X269I, X269R, X270C, X271F,X271V, X271I, X271P, X271H, X271M, X271T, X271L, X 271A, X272F, X272R, X272F, X273I, X273F，和X274G，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X16S, X18R, X20R, X22A, X24R, X43R/D, X45T, X76D, X101A, X103G, X104L, X111V, X128N, X148I，X230E，X242R，和X249R，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X1R，X230E, X271L, X115R, X20R, X249R, X235F, X27V/F/L, X75E, X82R, X18R, X269R, X43D, X43R, X76D, X45T, X212F, X242R, X24R, X78R, X9A, X22R, X121E, X244R, X28E, X30E, X4R,和 X241R，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X062E-X158E, X103G-X158E, X128N-X158E, X016S-X158E, X104L-X158E, X089P-X158E, XlllV-Xl58E, X022A-X158E, X101A-X158E, X148I-X158E, X129E-X158E, X022A-X089P，X016S-X089P，X062E-X089P, X062E-X271F, X158E-X271F, X186H-X271F, X129E-X271F, X111V-X271F, X209E-X271F, X016S-X271F, X188D-X271F, X022A-X271F, X159E-X271F, X104L-X271F, XlOlA-X271F,X089P-X271F, X128N-X271F, X103G-X271F, X148I-X271F, X249R-X271F, X062E-X159E, X016S-X159E, X128N-X159E， X148I-X159E， X111V-X159E, X089P-X159E， X022A-X159E，X129E-X159E, X103G-X159E，X104L-X159E，X158E-X159E，X101A-X159E，X158E-X249R, XlllV-X249R, X129E-X249R, X062E-X249R, X016S-X249R, X186H-X249R, X148I-X249R, X159E-X249R, X101A-X249R, X188D-X249R, X 104L-X249R, X209E-X249R, X022A-X249R, X 128N-X249R, X103G-X249R, X089P-X249R, X022A-X111V, X101A-X111V, X016S-X111V, X104L-X111V,X062E-XlIIV, X103G-X111V, X089P-X111V, X016S-X148I, X062E-X148I, X022A-X148I, X129E-X1481，X104L-X148I, X103G-X148I, X128N-X148I, X101A-X148I, X089P-X148I, X111V-X148I，X016S-X062E， X022A-X062E， X062E-X129E， X022A-X129E， X128N-X129E， X016S-X129E，X101A-X129E, X104L-X129E，X089P-X129E，X103G-X129E，X111V-X129E, X062E-X186H, X128N-X186H, X101A-X186H, X022A-X186H，X016S-X186H，X158E-X186H, X089P-X186H，X129E-X186H, X159E-X186H, X103G-X186H，X104L-X186H, X111V-X186H,X148I-X186H,X062E-X101A,X022A-X101A, X016S-X101A, X089P-X101A, X062E-X103G, X022A-X103G, X016S-X103G, XlOlA-X103G, X089P-X103G, X062E-X128N, X016S-X128N, X022A-X128N, X101A-X128N, X104L-X128N, X089P-X128N, X103G-X128N, X111V-X128N, X111V-X188D, X062E-X188D, X016S-X188D,X148I-X188D, X022A-X188D, X128N-X188D, X101A-X188D，X104L-X188D, X089P-X188D，X129E-X188D, X159E-X188D, X186H-X188D, X103G-X188D, X158E-X188D, X016S-X022A, X016S-X104L, X022A-X104L, X101A-X104L, X062E-X104L，X103G-X104L，X089P-X104L，X159E-X209E， X111V-X209E, X101A-X209E, X016S-X209E，X128N-X209E, X148I-X209E, X129E-X209E,X062E-X209E, X022A-X209E， X103G-X209E， X158E-X209E， X188D-X209E， X104L-X209E，X089P-X209E，和X186H-X209E，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。 本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X018R-X241R, X020R-X241R, X024R-X241R, X009A-X241R，X020R-X241R, X004R-X241R, X043R-X241R,X078R-X241R, X022R-X241R, X115R-X241R, X001R-X241R, X212F-X241R, X082R-X241R, X018R-X244R, X024R-X244R, X078R-X244R, X020R-X244R, X212F-X244R, X009A-X244R，X082R-X244R, X001R-X244R, X043R-X244R, X022R-X244R, X004R-X244R, X 115R-X244R, X241R-X244R, X242R-X244R, X001R-X004R, X009A-X022R，X018R-X022R, X020R-X022R, X004R-X022R, X001R-X022R, X024R-X242R, X018R-X242R, X004R-X242R, X020R-X242R, X212F-X242R, X082R-X242R,X078R-X242R, X001R-X242R, X009A-X242R，X022R-X242R, X115R-X242R, X043R-X242R, X241R-X242R, X018R-X212F, X022R-X212F, X004R-X212F, X024R-X212F, X001R-X212F, X115R-X212F，X020R-X212F, X009A-X212F, X043R-X212F, X078R-X212F, X082R-X212F, X009A-X078R, XO20R-X078R, X024R-X078R, X022R-X078R, X018R-X078R, X004R-X078R, X001R-X078R, X043R-X078R, X022R-X024R, X020R-X024R, X018R-X024R, X001R-X024R, X004R-X024R, X009A-X024R，X004R-X009A, X001R-X009A, X242R-X269R, X024R-X269R, X020R-X269R, X022R-X269R, X249R-X269R, X212F-X269R, X043R-X269R, X244R-X269R, X001R-X269R, X018R-X269R, X078R-X269R，X009A-X269R, X115R-X269R, X241R-X269R, X004R-X269R, X082R-X269R, X018R-X043R, X020R-X043R, X004R-X043R, X022R-X043R, X009A-X043R，X001R-X043R, X024R-X043R, X009A-X018R, X004R-X018R, XOO1R-X018R, X024R-X082R, X009A-X082R，X018R-X082R, X001R-X082R, XO78R-X082R, X020R-X082R, X022R-X082R, X004R-X082R, X043R-X082R, X043R-X249R, X020R-X249R, X004R-X249R, X018R-X249R, X009A-X249R，X212F-X249R, X022R-X249R, X024R-X249R,X 115R-X249R, X001R-X249R, X082R-X249R, X242R-X249R, X241R-X249R, X244R-X249R, X078R-X249R, X018R-X115R, X020R-X115R, X022R-X115R, X078R-X115R, X009A-X115R，X004R-X115R, X001R-X115R, X082R-X115R, X043R-X115R, X024R-X115R, X009A-X020R，X018R-X020R, XO04R-X020R, X001R-X020R, X009A-X271L，X020R-X271L, X024R-X271L, X244R-X271L, X241R-X271L, X043R-X271L, X022R-X271L, X249R-X271L, X212F-X271L, X115R-X271L, X242R-X271L,X078R-X271L, X004R-X271L, X269R-X271L, X001R-X271L, X018R-X271L，和 X082R-X271L，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X-43R, X020K-X062E，X024F-X116L，X020K-X024F，X024R-X174T, X024R-X118R, X024R-X235F, X024R-X086R, X024R-X086W, X078R-X118R, X033S-X118R, X033S-X235F, X209A-X24IR, X020R-X076D, X018R-X245R, X024R-X045T, X232V-X245R, X118R-X172V, X118R-X194T, XO08T-X024R, X235F-X243F, X018R-X103A, X018R-X104I, X086W-X118R, X086W-X243F, X086W-X209A, X024C-X033S, X024R-X232V, X024R-X243F, X024R-X239Q, X024R-X101G, X024R-X141G, X024R-X033S, X024R-X274I, X024R-X209A, X078R-X086W, X101G-X232V, X033S-X148F, X033S-X086W, X033S-X201S, X033S-X078R, X033S-X241R, X033S-X209A, X230E-X249R, X232V-X249R, X118R-X235F, X076D-X245R, X086W-X235F, X024R-X247H, X024R-X104A, X078R-X235F, XlO1G-X249R, X103A-X232V, X033S-X048T, X033S-X239T, X033S-X253A, X143A-X209A, X209A-X235F, X018R-X045T, X209A-X243F, X024R-X272P, X024R-X269C, X101G-X104I, X104I-X232V, X076D-X249R，和X024R-X076D，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X076D, X024R-X045T, X230E-X249R, X018R-X045T, X018R-X245R, X101G-X232V, X024R-X232V,X232V-X245R, X024R-X101G, X018R-X104I, X018R-X103A, X101G-X249R, X232V-X249R, X103A-X232V, X076D-X245R, X101G-X104I, X104I-X232V, X076D-X249R, X024R-X076D, X024F-X116L, X020K-X024F, X020K-X062E，X033S-X118R,X024R-X086W, X024R-X118R, X024R-X086R, X209A-X241R, X024R-X241R, X024R-X235F, X118R-X209A, X078R-X118R, X033S-X235F, X024R-X174T, X086W-X209A, X008T-X024R，X086W-X118R, X033S-X241R, X024R-X243F, X024R-X209A,X033S-X086W, X024R-X033S, X086W-X243F, X033S-X201S, X024R-X239Q, X078R-X086W, X235F-X243F, X118R-X172V, X033S-X148F, X033S-X078R，X033S-X243F, X024C-X033S, X118R-X194T，X033S-X209A, X024R-X141G, X024R-X274I, X086W-X235F, X015T-X033S, X209A-X235F, X024R-X247H, X078R-X235F, X024R-X104A, X033S-X048T, X118R-X235F, X033S-X253A, X143A-X209A, X033S-X239T, X209A-X243F, X024R-X272P，和 X024R-X269C，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X004R-X009A-X020R-X242R, X020R-X043R-X241R, X020R-X242R-X269R, X004R-X009A-X020R-X043R, X004R-X020R-X249R, X018R-X024R-X244R, X009A-X022R-X212F-X24IR, X020R-X043R-X269R,X018R-X024R-X242R, X004R-X009A-X043R-X241R, X020R-X043R-X244R, X020R-X022R-X242R，X004R-X020R-X043R, X004R-X009A-X020R-X043R-X242R, X020R-X043R-X242R, X020R-X043R-X242R-X249R, X020R-X212F-X249R, X004R-X009A-X241R, X001R-X009A-X043R, X020R-X043R-X249R, X009A-X020R-X043R-X241R, X020R-X022R-X043R, X020R-X249R-X269R, X020R-X022R-X241R, X004R-X009A-X024R-X043R-X241R, X009A-X043R-X078R, X004R-X020R-X024R-X244R, X020R-X022R-X078R-X242R, X020R-X024R-X242R-X249R, X004R-X009A-X078R-X241R,X009A-X043R-X078R-X242R, X004R-X020R-X024R, X009A-X043R-X212F, X020R-X043R-X212F，X024R-X078R-X212F, X009A-X020R-X024R-X043R, X009A-X022R-X043R-X078R, X020R-X022R-X212F-X241R, X020R-X043R-X212F-X241R, X009A-X043R-X241R, X020R-X043R-X271L, X020R-X022R-X078R-X241R, X020R-X024R-X043R-X242R, X020R-X022R-X043R-X241R, X009A-X020R-X043R-X212F, X004R-X009A-X020R-X024R-X242R, X020R-X043R-X249R-X271L, X020R-X022R-X024R-X242R, X009A-X022R-X078R-X212F, X020R-X043R-X242R-X271L, X009A-X022R-X078R-X212F-X241R, X004R-X020R-X024R-X249R, X020R-X022R-X271L, X020R-X022R-X043R-X212F, X004R-X020R-X024R-X043R-X242R, X004R-X020R-X024R-X043R, X004R-X009A-X022R-X078R-X212F, X020R-X022R-X078R-X212F-X241R,和 X020R-X022R-X269R，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。 本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X018R-X020R-X043D-X045T-X230E, XO18R-X043R-X045T-X242R-X249R, X024R-X043D-X249R, X018R-X020R-X045T, X020R-X024R-X076D-X249R, X024R-X043R-X230E-X242R, X018R-X024R-X043D-X230E, X020R-X076D, X018R-X024R-X043D-X076D-X249R, X024R-X043R-X076D-X249R, X018R-X024R-X045T-X242R, X020R-X043D-X076D-X230E-X249R, X020R-X043R-X045T-X242R, X018R-X024R-X076D-X249R, X018R-X020R-X024R-X043D-X045T-X233I-X242R, X024R-X043R-X230E, X018R-X020R-X043D, X043R-X242R-X249R, X020R-X043R-X045T-X230E, X043R-X076D-X242R-X249R, X020R-X024R-X045T-X230E-X242R, X024R-X045T-X076D-X230E-X242R-X249R, X024R-X045T, X024R-X043R-X045T-X076D-X230E-X249R, X018R-X024R-X043D-X045T-X249R, XO18R-X043R-X045T-X249R, X024R-X043R-X242R, X018R-X020R-X043R-X076D-X249R, X020R-X024R-X043D-X249R, X020R-X043R-X230E-X242R, X020R-X043R-X242R, X018R-X043R-X076D-X230E, X020R-X024R-X043D-X242R, X020R-X043R-X230E, X018R-X020R-X043R-X076D-X242R-X249R, X043D-X045T-X076D-X249R，X018R-X043R-X242R-X249R, X018R-X020R-X043R-X045T-X242R, X018R-X020R-X043D-X230E-X242R, X020R-X024R-X043R-X045T-X249R, X024R-X043R-X249R, X020R-X024R-X27E-X043R-X076D-X230E, X024R-X043R-X045T-X242R, X018R-X020R-X024R-X043R-X045T-X076D-X230E, X020R-X043R-X076D-X230E-X249R, X018R-X043R-X045T-X242R, X020R-X242R-X249R, X018R-X043R-X076D-X230E-X242R-X249R, X018R-X024R-X076D, X020R-X024R-X27R-X043D-X242R-X249R, X018R-X020R-X024R-X043D-X076D-X242R, X018R-X043R-X076D-X242R-X249R, XO18R-X024R-X043D-X230E-X249R, X018R-X020R-X043D-X249R, X018R-X020R-X043D-X045T-X076D-X242R, X024R-X043R-X076D-X230E-X242R, X020R-X024R-X38I-X043R-X045T-X076D-X242R-X249R, X018R-X020R-X043R, X018R-X024R-X045T-X230E-X242R, X018R-X020R-X249R, X024R-X043R-X076D, X018R-X020R-X024R-X043R-X045T-X076D-X249R, X018R-X043D-X045T-X076D-X242R-X249R, X024R-X043D-X242R-X249R, X018R-X020R-X024R-X043D-X045T-X242R, X020R-X024R-X043R-X076D, X018R-X020R-X043D-X045T-X230E-X242R, X020R-X024R-X043R-X045T-X076D-X242R-X249R, X018R-X043R-X045T-X076D-X242R, X018R-X020R-X043R-X076D-X230E-X242R, X018R-X024R-X043D-X249R, X018R-X024R-X043R-X045T-X230E-X249R, XO18R-X020R-X043R-X045T-X076D-X249R, X018R-X024R-X242R, X018R-X043R-X045T-X076D-X230E-X242R, X045T-X242R-X249R,XO18R-X024R-X043D-X242R, X018R-X020R-X043D-X045T-X240P, X024R-X043R-X045T-X242R-X249R, X018R-X024R-X30S-X31S-X32I-X33Q-X34V-X35F, X018R-X020R-X043R-X076D,X020R-X043D-X045T-X076D-X242R-X249R, X018R-X024R-X043D-X230E-X242R, X018R-X024R-X043D-X242R-X249R, X024R-X043D-X045T-X242R-X249R, X043R-X230E-X249R, X024R-X043R-X076D-X230E-X249R, X020R-X024R-X043D-X076D-X249R, X024R-X045T-X242R-X273V, X020R-X024R-X045T-X076D-X242R-X249R, X018R-X024R-X043D-X076D-X242R, X018R-X043R-X076D-X230E-X249R, XO18R-X020R-X043R-X045T-X249R, XO18R-X043R-X045T-X230E-X242R,X020R-X024R-X043D-X045T-X230E-X242R, X018R-X043D-X230E-X249R，X018R-X043R-X076D-X242R, X018R-X020R-X076D, X018R-X020R-X043D-X076D-X242R-X249R, X020R-X024R-X0 43D-X076D-X242R-X249R, X043D-X242R-X249R, XO18R-X020R-X024R-X043R-X076D, X018R-X020R-X043D-X045T-X076D-X249R, X018R-X020R-X043R-X045T-X076D-X230E-X249R, XO18R-X076D-X242R, X020R-X043R-X249R, XO18R-X076D-X242R-X249R, X018R-X024R-X045T-X230E-X249R, X230E-X249R, X018R-X045T-X249R, X020R-X043R-X076D, X043R-X045T-X249R,XO18R-X043D-X076D-X242R-X249R, X043R-X076D-X249R，X018R-X045T, X020R-X076D-X230E-X242R, X020R-X024R-X043D-X045T, X024R-X043D-X076D-X242R-X249R, X020R-X045T-X249R, X043R-X076D-X153A-X249R, X043R-X076D-X230E-X249R, X018R-X043D-X076D-X249R,和X020R-X043R-X076D-X227I，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列=XlOlG-Xl03A-X104I-X232V-X236H-X245R-X252K, X101G-X103A-X104I-X232V-X245R-X248R, XlOlG-X103A-X104I-X159R-X232V-X245R-X248D, X101G-X103A-X104I-X159D-X232V-X245R-X248R,XlO1G-X103A-X104I-X232V-X245R, X101G-X103A-X104I-X159D-X232V-X245R 和 X101G-X103A-X104I-X232V-X245R-X248D，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列=XlOlG-X103A-X104I-X129E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X159E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128N-X129E-X158E-X232V-X245R-X248D-X249R, X024R-X101G-X103Avcvyl-yv C _■ C _■ c· C Ce c· L -' C Ce C C K Kc C C vlcct-yv tec _■ ^ h -' C _■ vcvyl-yv C _■ C _■ Vccvyyv
Q8gx-HLO艺 χ-ΛΖ Χ-α88 χ-389 χ-Ι42χ-νε2χ-ο 2χ-νζδχ^6^χ-α8 艺 XIHS 艺 Χ-ΛΖ x-aococT—ix-sLOT—ix-sLOT—ix-IST—ix-vcooT—ix-oT—ioT—ix-vzsxQOC艺 X-HLO艺 χ-ΛΖ Χ-389 χ-Ι8 茳XII 寸 O χ-νεο χ-ο ο χ-等 Sx^6^χ-α8 艺 X-HLO艺 χ-ΛΖ Χ-389 χ-36ζ χ-Ι#2χ-νε2χ-ο 2χ-Η 寸Sx-VzsxQOC艺 χ-Η93χ-Η8 Χ-ΛΖ Χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-νζδχ C6^x-a84 - 艺 χ-ΛΖ Χ-α88 χ-369 χ-Ι42χ-νε2χ-ο ο χ-等 SxQocgx-HLO艺 χ-ΛΖ Χ-α88 χ-369 χ-389 χ-Ι42χ-νε2χ-ο 2χ-等 Sx-VzsxΩ83χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 χ-389 χ-36^χ-Ι#2χ-νε2χ-ο 2χ-等 Sx-VzsxQocgx-HLO艺 χ-ΛΖ Χ-369 χ-362χ-Ι#ο χ-νεο χ-ο ο χ-等 SxQocgx-HLOgx-Hocsx-i^sx-sdx-Nocdx-IST—ix-vsT—ix-oT—ioT—ix-vzsxQOC寸zx-hlo艺 χ-ΛΖ Χ-α88 χ- 369 χ-Ι42χ-νε2χ-ο 2χ-筇 SxQocgx-HLO艺 χ-ΛΖ Χ-389 χ-36dx-Nocdx-IST—ix-vcooT—ix-oT—ioT—ix-等 Sx-VzsxQocgx-HLO艺 χ-ΛΖ Χ-αοο8 χ-389 χ-36ζ χ-Ι寸 ο χ-νεο χ-ο ο χ-等 SxΩ83χ-Η93χ-ΛΖ Χ-α88 χ-Ν8ζ χ-Ι42χ-νε2χ-ο 2χ-等 Sx-VZSXQOC艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-369 χ-389 χ-Ι#2χ-νεο χ-ο ο χ-等 Sx-Η8 Χ-ΛΖ Χ-α88 χ-362χ-Ι42χ-νε2χ-ο 2χ-等 SxΩ83χ-Η93χ-Η8 Χ-ΛΖ Χ-α88
x-g^x-Noc^x-IST—ix-vcooT—ix-oT—ioT—ix-vzsxΩ83χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 χ-389 χ-Ι 寸 O
χ-νεο χ-ο ο χ-等 SxQOC艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-筇 Sx-VS0XQOC艺 X-HLO艺 χ-ΛΖ Χ-362χ-Ι40 χ-νεο χ-ο ο χ-等 Sx-VzsxΩ83Χ-Η93Χ-ΛΖ Χ-389 χ-36ζ χ-ΙΜΠΧ-νεο χ-ο 2χ δχ-νζδχΩ8 艺 x-hlo艺 χ-ΛΖ Χ-αοο8 χ-36ζ χ-Ι 寸 O χ-νεο χ-ο ο χ-筇 SXQOC艺 χ-Η9$χ-ΛΖ Χ-α88 χ-389 χ-Ν8ζ χ-ΙΜΗΧ-νε2χ-ο 2χ-Η寸 Sx-Vzsx^6^x-aoc艺 X-HLO艺 χ-ΛΖ Χ-369 χ-389 χ-362χ-Ι#ο χ-νεο χ-ο ο χ-等 Sx-VZSXΩ83χ-Η93χ-Η8 Χ-ΛΖ Χ-369 χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-νζδχ ^zx-aoc艺 X-HLO艺 χ-ΛΖ Χ-369 χ-389 χ-Ι42χ-νεο χ-ο ο χ^63χ-α83χ-Η93χ-ΛΖ Χ-α88 χ-389 χ-ΙΜΠΧ-νεο χ-ο ο χ-等 SX-S20XQOC艺 X-HLO艺 χ-ΛΖ Χ-389 χ-Ι8^χ-36ζ χ-I 寸 ο χ-νεο χ-ο ο χ-筇 SXQOC艺 X-HLO艺 χ-Η8 Χ-ΛΖ Χ-αοο8 χ-369 χ-389 χ-362χ-Ι 寸 O χ-νεο χ-ο ο χ-筇 Sx-Vzsx^63χ-α83χ-Η93χ-ΛΖ Χ-α88 χ-369 χ-389 χ-Ι40 χ-νεο χ-ο ο χ-等 Sx-Vzsx C6^x-a0c艺 X-HLO艺 χ-ΛΖ Χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-H 寸 Sx^6^x-aoc艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-362χ-Ι40 χ-νεο χ-ο ο χQ8gx-HLOgx-i\zcoζχ-36ζ χ-Ν8ζ χ-Ι^ χ-νε2χ-ο 2χ δχ^6^x-aoc艺 X-HLO艺 χ-ΛΖ Χ-αοο8 χ-369 χ-3sT—ix-IST—ix-vcooT—ix-oT—ioT—ix-等 Sx-VzsxΩ83χ-Η93χ-ΛΖ Χ-α88 χ-389 χ-Ι8^χ-Ι#2χ-
X sLn/z F ^ S V 80ZCOCO6SI δ159E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X128N-X159E-X188D-X232V-X245R-X248D, X022A-X101G-X103A-X104I-X159E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X159E-X188D-X232V-X238R-X245R-X248D, X101G-X103A-X104I-X158E-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X129E-X159E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X148I-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X158E-X159E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X158E-X159E-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X129E-X158E-X159E-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X129E-X158E-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X159E-X232V-X245R-X248D, X101G-X103A-X104I-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X148I-X158E-X232V-X245R-X248D, X101G-X103A-X104I-X129E-X158E-X159E-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X159E-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X159E-X188D-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X129E-X232 V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X129E-X158E-X159E-X232V-X245R-X248D-X249R, X022A-X10IG-Xl03A-X104I-X158E-X159E-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X159E-X232V-X238R-X245R-X248D,X101G-X103A-X104I-X128N-X129E-X232V-X245R-X248D, X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X232V-X245R-X248D-X249R,X101G-X103A-X104I-X129E-X159E-X232V-X238R-X245R-X248D, X101G-X103A-X104I-X158E-X159E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X129E-X148I-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X232V-X238R-X245R-X248D, X101G-X103A-X104I-X128N-X129E-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X128N-X159E-X232V-X245R-X248D, X022A-X101G-X103A-X104I-X128N-X129E-X158E-X232V-X238R-X245R-X248D, Xl01G-X103A-X104I-X128N-X129E-X188D-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X128N-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128N-X158E-X159E-X188D-X232V-X245R-X248D, XO22A-XO24K-XIOIG-XIO3A-XI04I-X128N-X158E-X159E-X232V-X245R-X248D, X101G-X103A-X104I-X129E-X148I-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X148I-X232V-X245R-X248D, X022A-X101G-X103A-X104I-X148I-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X128N-X129E-X188D-X232V-X245R-X248D, X101G-X103A-X104I-X128N-X129E-X158E-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X148I-X158E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X148I-X158E-X188D-X232V-X245R-X248D, Xl01G-X103A-X104I-X148I-X159E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D，和 X101G-X103A-X104I-X128N-X129E-X158E-X232V-X245R-X248D-X249R，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X022A-X024R-X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X129E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X158E-X159E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X159E-X188D-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X158E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X159E-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X148I-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X148I-X158E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D-X249R, X016S-X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X02 4R-X101G-X103A-X104I-X129E-X159E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X129E-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X148I-X158E-X232V-X245R-X248D,X024R-X101G-X103A-X104I-X129E-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X159E-X188D-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X158E-X159E-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D, X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, XO22A-X101G-X103A-X104I-X158E-X159E-X232V-X245R-X248D-X249R，和 X024R-X101G-X103A-X104I-X158E-X159E-X232V-X245R-X248D，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所不解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X104L-X128N-X158E-X186H-X249R, X128N-X158E-X188D-X249R, X062E-X128N-X158E-X159E-X271F，X062E-X158E-X188D-X249R-X271F, X062E-X158E-X186H-X249R-X271F，X128N-X158E-X188D-X209E-X271F, X062E-X159E-X188D-X249R, X016S-X062E-X158E-X186H-X249R，X062E-X158E-X159E-X249R, X101A-X128N-X158E-X209E-X249R，X128N-X158E-X186H-X27IF, X062E-X158E-X188D-X249R, X062E-X158E-X186H-X271F, X062E-X158E-X186H-X249R, X062E-X101A-X186H-X249R, X062E-X101A-X158E-X186H-X271F, X062E-X104L-X158E-X188D-X249R-X271F, X062E-X159E-X186H-X249R, X062E-X159E-X249R，X128N-X158E-X186H-X249R，X128N-X158E-X188D-X271F, X062E-X158E-X249R，X062E-X186H-X188D-X249R-X271F，X128N-X158E-X209E-，X062E-X101A-X158E-X249R, X104L-X128N-X158E-X186H-X271F，X062E-X101A-X158E-X186H-X249R-X271F, X016S-X062E-X158E-X249R，X062E-X101A-X159E-X249R，X128N-X158E-X186H-X188D-X271F, X101A-X128N-X158E-X186H-X271F，X062E-X101A-X188D-X249R，XlOlA-X104L-X158E-X186H-X188D-X249R, X062E-X159E-X249R-X271F, X128N-X158E-X159E-X271F,X016S-X062E-X104L-X158E-X186H-X271F, X022A-X128N-X158E-X249R，X128N-X158E-X249R，X062E-X101A-X104L-X158E-X186H-X271F, X016S-X062E-X158E-X186H-X271F，X104L-X128N-X158E-X249R, X104L-X128N-X158E-X188D-X249R, X022A-X062E-X158E，X062E-X101A-X188D-X249R-X271F, X062E-X158E-X249R-X271F, X104L-X128N-X158E-X186H-X188D-X271F, XO62E-X101A-X186H-X271F, X062E-X104L-X159E-X249R，X062E-X186H-X249R，X062E-X101A-X186H-X249R-X271F, X101A-X158E-X186H-X188D-X249R，X062E-X101A-X186H，X101A-X128N-X129E-X186H-X249R, X101A-X103G-X158E-X186H-X249R, X016S-X062E-X104L-X186H-X188D-X271F, X104L-X158E-X186H-X249R, X101A-X128N-X158E-X188D-X209E-X271F，X062E-X101A-X186H-X188D-X271F, X016S-X062E-X158E-X249R-X271F，X062E-X128N-X158E，X062E-X128N-X159E-X249R, X062E-X101A-X158E-X188D-X249R, X101A-X128N-X158E-X249R, X062E-X158E-X186H-X188D-X249R, X016S-X104L-X158E-X186H-X271F，X062E-X148I-X159E，X062E-XlOlA-Xl58E-X186H-X249R, X062E-X101A-X186H-X188D-X249R, X104L-X158E-X186H-X188D-X249R, X062E-X101A-X104L-X186H-X188D-X271F, X022A-X101A-X158E-X186H-X249R，XlO1A-X128N-X158E-X209E, X158E-X186H-X188D-X249R-X271F，X104L-X158E-X186H-X188D-X249R-X271F, X101A-X104L-X158E-X186H-X249R，X104L-X158E-X249R，X101A-X104L-X128N-X 158E-X186H-X271F, X016S-X104L-X188D-X249R, X101A-X104L-X158E-X186H-X188D-X271F,X104L-X128N-X159E-X271F, X104L-X158E-X186H-X249R-X271F，X158E-X186H-X249R，XlOlA-X158E-X186H-X249R, X104L-X158E-X188D-X249R-X271F，X016S-X128N-X158E-X186H，X104L-X128N-X186H-X188D-X249R, X016S-X101A-X128N-X186H, X016S-X062E-X128N-X186H-X27IF, X016S-X128N-X186H-X271F, X128N-X129E-X186H，X158E-X186H-X249R-X271F，X016S-X158E-X249R, X016S-X158E-X186H-X249R，X016S-X022A-X158E-X186H-X271F，X089P-X101A-X129E-X186H,X022A-X128N-X158E-X186H, X101A-X104L-X128N-X158E-X186H, X022A-X128N-X186H-X188D-, X062E-X104L-X158E-X186H-X188D-X249R, X022A-X158E-X186H-X249R-X27IF, X022A-X104L-X158E-X249R, X101A-X111V-X129E, X016S-X158E-X249R-X271F，X016S-X111V-X188D-, X022A-X104L-X186H-X188D-X249R，和 X104L-X148I-X188D-X249R，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列=XOOlR-X101G-X103A-X104I-X232V-X245R, X004R-X101G-X103A-X104I-X232V-X245R, X043R-X101G-X103A-X104I-X232V-X245R-X271L, X078R-X101G-X103A-X104I-X232V-X245R, X004R-X043R-X101G-X103A-X104I-X232V-X245R, X018R-X043R-X101G-X103A-X104I-X232V-X245R, X020R-X101G-X103A-X104I-X232V-X245R, X101G-X103A-X104I-X232V-X245R-X271L, X020R-X043R-X101G-X103A-X104I-X232V-X245R, X024R-X043R-X101G-X103A-X104I-X232V-X245R, XO20R-X025R-X116A-X167W, X018R-X101G-X103A-X104I-X232V-X245R, X022R-X101G-X103A-X104I-X232V-X245R, X078R-X103N-X106G-X167W-X236N, X018R-X043D-X101G-X103A-X104I-X232V-X245R-X269R, X043R-X101G-X103A-X104I-X232V-X245R-X269R, X024R-X101A-X120F-X194F-X249R, X020R-X043D-X101G-X103A-X104I-X232V-X245R-X269R, X101G-X103A-X104I-X212F-X232V-X245R, X020R-X144R-X185I-X233C-X236N, X023A-X078R-X216F-X236N-X249R, X101G-X103A-X104I-X232V-X245R-X269R, X101G-X103A-X104I-X115R-X232V-X245R, XO52N-X078R-X103N-X148I-X213A, X018R-X043D-X101G-X103A-X104I-X232V-X245R-X249R, X024R-X043D-X101G-X103A-X104I-X232V-X245R-X249R, X024R-X043D-X101G-X103A-X104I-X232V-X245R-X269R, X025R-X089I-X116A-X239S-X270C, X024R-X101G-X103A-X104I-X232V-X245R, X148I-X213A-X252R, X024R-X025R-X183D-X192W-X239S, X046R-X194F-X212M, X104L-X217E-X224A-X249R-X252R, X023A-X091F-X121F-X192W-X236N, X101G-X103A-X104I-X232V-X244R-X245R, X099F-X144R-X167W-X252R, X101G-X103A-X104I-X232V-X245R-X249R, XO43R-X101G-X103A-X104I-X232V-X245R, X022W-X078R-X167W-X212M-X270C, X121F-X252R-X270C,X020R-X103N-X216F-X236N-X252R, X043R-X101G-X103A-X104I-X232V-X245R-X249R，X023A-X052N-X192W-X198L-X252R, X025R-X046R-X121F, X024R-X078R-X104L-X116A-X183D, X046R-X059A-X103N-X211Q-X212M, X020R-X052N-X062Q-X091F-X192W, X023A-X052N-X144R-X192W-X216F, X101G-X103A-X104I-X232 V-X242R-X245R, XO52N-XIO3N-X116A-X1481-X192W, X089I-X116A-X117F-X224A-X249R, X144R-X211Q-X238L-X239S-X249R, X043A-X062Q-X194F-X211Q, X020R-X024R-X052N-X059A-X216F, X024R-X167W-X224A-X249R, X057R-X167W-X249R, X025R-X103N-X186K-X194F-X224A，X105T-X128N-X144R-X148I-X212M，X020R-XO59A-X144R-X192W-X224A, XO24R-X043A-X117F-X194F-X211Q, X117F-X194F-X213A-X270C, X078R-X091F-X121F-X233C-X252R, X057R-X099F-X105T-X198L-X213A, X023A-X091F-X101A-X198L-X252R, X062Q-X103N-X121F-X144R-X249R, X043R-X101G-X103A-X104I-X232V-X242R-X245R, X023A-X024R-X117F-X212M-X216F，X104L-X213A-X216F，X194F-X211Q-X236N, X062Q-X103N-X117F-X194F, X024R-X062Q-X104L-X106G-X249R, X057R-X089I-X198L，X046R-X059A-X106G-X217E-X249R, X117F-X213A-X215F, X101A-X120F-X192W-X215F-X224A,X043A-X057R-X117F-X144R-X183D, X046R-X183D-X238L, X025R-X043A-X089I-X117F, X078R-X104L-X213A-X215F-X224A, X091F-X099F-X101A-X105T-X167W, X106G-X117F-X238L，X046R-X089I-X091F-X101A-X116A, X020R-X062Q-X0891-X186K-X212M, X057R-X099F-X12IF-Xl85I-X192W, X046R-X089I-X192W-X233C-X270C, X089I-X117F-X185I-X215F-X233C，X052N-X104L-X183D-X216F-X249R, X078R-X099F-X116A-X186K-X224A, X025R-X105T-X128N-X144R-X270C, X105T-X211Q-X216F, X024R-X046R-X091F-X121F，X106G-X185I-X216F-X236N，X062Q-X101A-X236N-X252R-X270C, X025R-X043A-X091F-X198L-X270C, X020R-X023A-X104L-X192W-X233C, X024R-X043A-X105T-X106G-X198L, X020R-X089I-X217E，X024R-X091F-X198L-X215F-X239S, X046R-X0891-X099F-X186K-X212M, X104L-X120F-X186K-X216F-X252R，X022W-X194F-X213A-X233C-X238L, X099F-X105T-X106G-X194F-X212M，X089I-X105T-X116A-X215F-X216F, X025R-X116A-X120F-X224A-X270C, X043A-X059A-X101A-X216F-X224A，X057R-X183D-X236N, X025R-X062Q-X128N-X144R-X185I, X103N-X120F-X167W-X198L-X233C, X022W-X089I-X216F, X024R-X106G-X116A-X212M-X224A, X020R-X052N-X101A-X198L-X233C, X089I-X091F-X185I-X211Q-X270C, X111I-X215F-X239S, X024R-X116A-X186K-X233C-X236N,和X023A-X103N-X106G-X212M-X215F，其中该枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所不解淀粉芽抱杆菌枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。本发明还提供分离的枯草杆菌蛋白酶变体，其中该枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X0
权利要求
1.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-N043R，N062E-A158E, S103G-A158E, S128N-A158E，A016S-A158E，V104L-A158E，E089P-A158E，L111V-A158E, T022A-A158E，S101A-A158E，L148I-A158E，P129E-A158E，T022A-E089P, AOl6S-E089P, N062E-E089P, N062E-E271F, A158E-E271F, R186H-E271F, P129E-E271F, LlllV-E·271F, Y209E-E271F, A016S-E271F, S188D-E271F, T022A-E271F, G159E-E271F, V104L-E271F，S101A-E271F, E089P-E271F, S128N-E271F, S103G-E271F, L148I-E271F, H249R-E271F, N062E-G159E, A016S-G159E, S128N-G159E, L148I-G159E, L111V-G159E, E089P-G159E, T022A-G1·59E, P129E-G159E, S103G-G159E，V104L-G159E，A158E-G159E，S101A-G159E, A158E-H249R,L111V-H249R, P129E-H249R, N062E-H249R，A016S-H249R，R186H-H249R，L148I-H249R, G159E-H249R, S101A-H249R, S188D-H249R, V104L-H249R, Y209E-H249R, T022A-H249R, S128N-H2·49R, S103G-H249R, E089P-H249R，T022A-L111V, S101A-L111V, A016S-L111V, V104L-L111V,N062E-L111V,S103G-L111V, E089P-L111V, A016S-L148I, N062E-L148I, T022A-L148I, P129E-L148I, V104L-L148I, S103G-L148I, S128N-L148I, S101A-L148I, E089P-L148I, L111V-L14··81，A016S-N062E，T022A-N062E，N062E-P129E, T022A-P129E, S128N-P129E, A016S-P129E, S·101A-P129E, V104L-P129E, E089P-P129E, S103G-P129E, L111V-P129E, N062E-R186H, S128N-R186H, S101A-R186H, T022A-R186H, A016S-R186H, A158E-R186H, E089P-R186H, P129E-R18·6H, G159E-R186H, S103G-R186H, V104L-R186H, L111V-R186H, L148I-R186H, N062E-S101A，T·022A-S101A, A016S-S101A, E089P-S101A，N062E-S103G，T022A-S103G, A016S-S103G，SlOlA-S103G, E089P-S103G, N062E-S128N, A016S-S128N, T022A-S128N, S101A-S128N, V104L-S12·8N, E089P-S128N, S103G-S128N, L111V-S128N, L111V-S188D, N062E-S188D，A016S-S188D，L·148I-S188D, T022A-S188D, S128N-S188D, S101A-S188D，V104L-S188D, E089P-S188D，P129E-S188D, G159E-S188D, R186H-S188D，S103G-S188D, A158E-S188D, A016S-T022A, A016S-V10·4L, T022A-V104L, S101A-V104L, N062E-V104L, S103G-V104L, E089P-V104L, G159E-Y209E，L111V-Y209E, S101A-Y209E，A016S-Y209E，S128N-Y209E, L148I-Y209E, P129E-Y209E,N062E-Y209E, T022A-Y209E，S103G-Y209E，A158E-Y209E，S188D-Y209E，V104L-Y209E，E089P-Y209E, R186H-Y209E，N018R-W241R, G020R-W241R, S024R-W241R, S009A-W241R, G020R-W241R, V004R-W241R, N043R-W241R, S078R-W241R, T022R-W241R, G115R-W241R, A001R-W2·41R, S212F-W241R, L082R-W241R, N018R-V244R, S024R-V244R, S078R-V244R, G020R-V244R,S212F-V244R, S009A-V244R, L082R-V244R, A001R-V244R, N043R-V244R, T022R-V244R, V004R-V244R, G115R-V244R, W241R-V244R, S242R-V244R, A001R-V004R, S009A-T022R, N018R-T0·22R, G020R-T022R, V004R-T022R, A001R-T022R, S024R-S242R, N018R-S242R, V004R-S242R,G020R-S242R, S212F-S242R, L082R-S242R, S078R-S242R, A001R-S242R, S009A-S242R，T022R-S242R, G115R-S242R, N043R-S242R, W241R-S242R, N018R-S212F, T022R-S212F, V004R-S2·12F, S024R-S212F, A001R-S212F, G115R-S212F, G020R-S212F, S009A-S212F，N043R-S212F,S078R-S212F, L082R-S212F, S009A-S078R，G020R-S078R, S024R-S078R, T022R-S078R, NO 18R-S078R, V004R-S078R, A001R-S078R, N043R-S078R, T022R-S024R, G020R-S024R, N018R-S0·24R, A001R-S024R, V004R-S024R, S009A-S024R，V004R-S009A, A001R-S009A, S242R-N269R,S024R-N269R, G020R-N269R, T022R-N269R, H249R-N269R, S212F-N269R, N043R-N269R, V244R-N269R, A001R-N269R, N018R-N269R, S078R-N269R, S009A-N269R，G115R-N269R, W241R-N269R, V004R-N269R, L082R-N269R, N018R-N043R, G020R-N043R, V004R-N043R, T022R-N043R,S009A-N043R, A001R-N043R, S024R-N043R, S009A-N018R，V004R-N018R, A001R-N018R, S024R-L082R, S009A-L082R, N018R-L082R, A001R-L082R, S078R-L082R, G020R-L082R, T022R-L082R, V004R-L082R, N043R-L082R, N043R-H249R, G020R-H249R, V004R-H249R, N018R-H249R,S009A-H249R, S212F-H249R, T022R-H249R, S024R-H249R, G 115R-H249R, A001R-H249R, L082R-H249R, S242R-H249R, W241R- H249R, V244R-H249R, S078R-H249R, N018R-G115R,G020R-G115R, T022R-G115R, S078 R-G115R, S009A-G115R，V004R-G115R, AOOlR-Gl15R, L082R-G115R,N043R-G115R, S024R-G115R, S009A-G020R，N018R-G020R,V004R-G020R,A001R-G020R,S009A-E271L, G020R-E271L, S024R-E271L, V244R-E271L, W241R-E271L, N043R-E271L, T022R-E271L, H249R-E271L, S212F-E271L, G115R-E271L, S242R-E271L, S078R-E271L, V004R-E271L,N269R-E271L, A001R-E271L, N018R-E271L，和 L082R-E271L，并且其中所述变体的总净电荷相对于迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的总净电荷为O、+1、+2、+3、+4、+5、-I、_2、_3、-4或-5,并且其中所述总净电荷是通过一种或多种选自以下的置换来获得的N43D, R45T, N62E, N76D, S101D, P129E, A158E, G159D, G159E, S166D, S188D, A230E, N18R, G20K, G20R, T22R, S24R, N43R, G118R, Q245R, H249R, N269R, E271F,和E271L，并且其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所示解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号。
2.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-N043R，G020K-N062E, S024F-N116L，G020K-S024F，S024R-A174T, S024R-G118R, S024R-K235F, S024R-P086R, S024R-P086W, S078R-G118R, T033S-G118R, T033S-K235F, Y209A-W241R, G020R-N076D, N018R-Q245R, S024R-R045T, A232V-Q245R, G118R-A172V, G118R-A194T, I008T-S024R，K235F-N243F, N018R-S 103A, N018R-V104I,P086W-G118R, P086W-N243F, P086W-Y209A, S024C-T033S, S024R-A232V, S024R-N243F, S024R-P239Q, S024R-S101G, S024R-S141G, S024R-T033S,S024R-T274I, S024R-Y209A, S078R-P086W, S101G-A232V, T033S-L148F, T033S-P086W, T033S-P201S, T033S-S078R, T033S-W241R, T033S-Y209A, A230E-H249R, A232V-H249R, G118R-K235F，N076D-Q245R,P086W-K235F,S024R-R247H,S024R-V104A,S078R-K235F,S101G-H249R,SI03A-A232V, T033S-A048T, T033S-P239T, T033S-T253A, T143A-Y209A，Y209A-K235F, N018R-R045T,Y209A-N243F, S024R-A272P, S024R-R269C, S101G-V104I, V104I-A232V, N076D-H249R，和S024R-N076D，并且其中所述变体的总净电荷相对于迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的总净电荷为O、+1、+2、+3、+4、+5、-I、-2、-3、-4或-5，并且其中所述总净电荷是通过一种或多种选自以下的置换来获得的N43D，R45T, N62E, N76D, S101D, P129E, A158E, G159D, G159E, S166D, S188D, A230E, N18R, G20K, G20R, T22R, S24R, N43R,G118R, Q245R, H249R, N269R, E271F，和E271L，并且其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所不解淀粉芽抱杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号。
3.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-N076D，S024R-R045T, A230E-H249R, N018R-R045T, N018R-Q245R, S 101G-A232V, S024R-A232V, A232V-Q245R, S024R-S101G, N018R-V104I, N018R-S103A, S101G-H249R，A232V-H249R, S103A-A232V, N076D-Q245R, S101G-V104I, V104I-A232V, N076D-H249R, S024R-N076D, S024F-N116L, G020K-S024F, G020K-N062E, T033S-G118R, S024R-P086W, S024R-G118R, S024R-P086R, Y209A-W241R, S024R-W241R, S024R-K235F, G118R-Y209A, S078R-G118R, T033S-K235F, S024R-A174T, P086W-Y209A, I008T-S024R，P086W-G118R, T033S-W241R, S024R-N243F, S024R-Y209A, T033S-P086W, S024R-T033S, P086W-N243F, T033S-P201S,S024R-P239Q,S078R-P086W,K235F-N243F，G118R-A172V, T033S-L148F, T033S-S078R, T033S-N243F, S024C-T033S, G118R-A194T, TO33S-Y209A, S024R-S141G, S024R-T274I, P086W-K235F, A015T-T033S, Y209A-K235F, S024R-R247H, S078R-K235F, S024R-V104A, T033S-A048T, G118R-K235F, T033S-T253A, T143A-Y209A, T033S-P239T, Y209A-N243F, S024R-A272P,和 S024R-R269C，并且其中所述变体的总净电荷相对于迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的总净电荷为O、+1、+2、+3、+4、+5、-I、_2、_3、-4或-5,并且其中所述总净电荷是通过一种或多种选自以下的置换来获得的N43D, R45T, N62E, N76D, S101D, P129E, A158E, G159D, G159E, S166D, S188D,A230E, N18R, G20K, G20R, T22R, S24R, N43R, G118R, Q245R, H249R, N269R, E271F,和 E271L,并且其中所述蛋白酶变体的氨基酸位置根据SEQ IDNO:l所示解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号。
4.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020K-G023A-N043W-G118R-S128I-P129E-G159D-S188D, G020K-G023A-N062E-N116L, G020K-G023A-N062E-N116L-S188D, G020K-G023A-N062E-N116L-S188D-T213A, G020K-G100S-N116L-A158E-S166D-N243F, G020K-N043W-N062E-N116L-S 188D,G020K-N062E, G020K-N062E-N116L, G020K-N062E-N116L-S188D, G020K-N062E-N116L-T213A, G020K-N062E-S188D-T213A, G020K-S024F-N062E-N116L-G118R-S 188D, G020K-S024F-N062E-N116L-S188D, G020K-S024F-N062E-S188D-T213A, G023A-N062E-N116L-S188D, G023A-S024F-N062E-N116L-T213A, N043R-N076D-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R-E271F, N043R-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, N043W-N062E-N116L, N043W-S101D-S212M-N243F, N062E-N116L-G118R-S188D, N062E-N116L-G118R-T213A, N062E-N116L-S188D-T213A, S024F-G118R-S128I-P129E-G159D, S024F-N062E-N116L-S188D, S024R-S101G-S103A-V104I-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S 101G-S103A-V104I-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-S130A-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, SO24R-S101G-S103A-V104I-S130A-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S101D-S103N-N116L-S144R-A215D, S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A -V104I-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, T022A-N043R-S101G-S103A-V104 I-G159D-S188D-L217E-A232V-Q245R-N248D-E271F, T022A-N043R-S103A-V104I-G118R-S12 8I-P129E-G159D-S188D-A232V-N248D-E271F, T022A-N043R-S103A-V104I-S128I-P129E-G1 59D-S188D-A232V-Q245R-N248D, T022A-S024R-S103A-V104I-G118R-S128I-P129E-G159D-S 188D-A232V-N248D, T022A-S101G-S103A-V104I-G159D-L217E-A232V-Q245R-N248D-E271F, T022A-S101G-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F, T022L-T038F-A048R -N062E-G100S-R186K, T033S-N043W-N218D-P239G-N243F, V026F-A048R-S105T-T213A-N218 D-T224A, V026F-L031F-S078N-G102A-S160D, VO26F-V05lff-V104L-S106E, V104L-S105T-T21 3A-L217E-S256N, A016S-N062E-A158E-H249R，A016S-N062E-A158E-R186H-E271F，A016S-N0 62E-A158E-R186H-H249R, A016S-N062E-V104L-A158E-R186H-E271F, A016S-S024R-S101G-S 103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, G020K-G023A-N062E-S188D，G020K-G023A-S024F-N062E-G118R-S188D-T213A, G020K-N043W-N062E-N116L-S188D-T213A-P239G ，G020K-S024F-N062E-S188D-P239G, G023A-N062E-N116L-G118R, G023A-N062E-N116L-G118 R-S188D-P239G, G023A-S024F-N062E-N116L-G118R, H017R-T022A-N076D-S101G-S103A-V10 4I-G159D-S188D-A232V-Q245R-N248D-E271F, N043R-N076D-S101A-S103A-V104I-A158E-S1 88D-A232V-Q245R-N248D-H249R-E271F, N062E-A158E-G159E-H249R, N062E-A158E-H249R, N 062E-A158E-R186H-E271F, N062E-A158E-R186H-H249R, N062E-A158E-R186H-H249R-E271F,N062E-A158E-S188D-H249R, N062E-A158E-S188D-H249R-E271F, N062E-G159E-H249R，N062E -G159E-H249R-E271F, N062E-G159E-R186H-H249R, N062E-G159E-S188D-H249R, N062E-R186 H-S188D-H249R-E271F, N062E-S101A-A158E-H249R，N062E-S101A-A158E-R186H-E271F，N06 2E-S101A-A158E-R186H-H249R-E271F, N062E-S101A-G159E-H249R，N062E-S101A-R186H-H2 49R, N062E-S101A-S188D-H249R, N062E-S101A-S188D-H249R-E271F，N062E-S101A-V104L-A 158E-R186H-E271F, N062E-S128N-A158E-G159E-E271F，N062E-V104L-A158E-S188D-H249R-E271F, N076D-S101G-S103A-V104I-A232V-M222Q-Q245R, S024F-N062E-N116L-P239G, S024F -N062E-S188D-T213A, S024F-N116L, S024R-N043R-N076D-S101A-S103A-V104I-A158E-S188 D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-G159E-A232V-N23 8R-Q245R-N248D, S024R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D, SO 24R-S101G-S103A-V104I-A158E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V 104I-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-G159E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-G159E-S188D-A232V-Q245R-N248D, S024R-S101G -S103A-V104I-G159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-L148 I-A158E-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-L148I-A158E-S188D-A232V-Q24 5R-N248D, S024R-S101G-S103A-V104I-L148I-A232V-Q245R-N248D, S024R-S101G-S103A-V1 04I-P129E-A158E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-A158E-G 159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-A158E-S 188D-A232V-Q245R-N248 D-H249R, S024R-S101G-S103A-V104I-P129E-G159E-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-G159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P1 29E-L148I-A158E-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-S188D-A232V-N 238R-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I -S128N-A158E-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-S128N-P129E-A158E-A232 V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128N-P129E-A232V-Q245R-N248D, S10 1A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101A-S103A-V104I-A158E-S1 88D-A232V-Q245R-N248D-H249R-E271F-E271F, S101A-S103A-V104L-A158E-S188D-A232V-Q 245R-N248D-H249R, S101A-S128N-A158E-R186H-E271F, S101A-S128N-A158E-Y209E-H249R, S101A-V104L-A158E-R186H-S188D-H249R, S101G-S103A-V104I-A158E-A232V-Q245R-N248D -H249R, S101G-S103A-V104I-A158E-G159E-A232V-Q245R-N248D-H249R, S101G-S103A-V104 I-A158E-S188D-M222Q-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-A158E-S188D-M22 2S-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-A232V-M222Q-Q245R, S101G-S103A-V1 04I-P129E-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129E-G159E-A 232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129E-S188D-A232V-N238R-Q245R-N248D, S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S188D -A232V-Q245R-N248D-H249R, S101G-S103G-V104I-A158E-S188D-A232V-Q245R-N248D-H249 R, S101G-S103S-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101S-S103A-V104I-A15 8E-S188D-A232V-Q245R-N248D-H249R, S101S-S103G-V104I-A158E-S188D-A232V-Q245R-N2 48D-H249R, S101S-S103G-V104V-A158E-S188D-A232V-Q245R-N248D-H249R, S128N-A158E-G 159E-E271F, S128N-A158E-H249R, S128N-A158E-R186H-E271F, S128N-A158E-R186H-H249R, S128N-A158E-R186H-S188D-E271F, S128N-A158E-S188D-E271F, S128N-A158E-S188D-H249R ，S128N-A158E-S188D-Y209E-E271F, S128N-A158E-Y209E-，T022A-N043R-S103A-V104I-G11 8R-P129E-G159D-S188D-A232V-Q245R-N248D, T022A-N062E-A158E，T022A-S024R-S101D-S1 03A-V104I-G118R-G159D-S188D-A232V-N248D-E271F, T022A-S024R-S101D-S103A-V104I-G 118R-P129E-G159D-S188D-A232V-Q245R-N248D, T022A-S024R-S101D-S103A-V104I-G159D-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-A158E-A232V-N238R-Q245R -N248D, T022A-S024R-S101G-S103A-V104I-A158E-A232V-Q245R-N248D-H249R, T022A-S024 R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-N238R-Q245R-N248D, T022A-S024R-S10 1G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V1 04I-A158E-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P 129E-A158E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-A158E-G159E-A232V-Q245R -N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-A158E-G159E-S188D-A232V-N238 R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-N238R-Q24 5R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248D-H2 49R, T022A-S024R-S101G-S103A-V104I-P129E-A232V-Q245R-N248D, T022A-S024R-S101G-S 103A-V104I-P129E-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-S128N-A158E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-S128N-P129E-A158E-A232V-Q245R-N248D, T02·2A-S024R-S101G-S103A-V104I-S128N-P129E-S188D-A232V-Q245R-N248D, T022A-S024R-S1·01G-S103A-V104I-S128N-S188D-A232V-Q245R-N248D, T022A-S024R-S103A-V104I-G118R-G·159D-S188D-L217D-A232V-N248D, T022A-S024R-S103A-V104I-P129E-G159D-S188D-A232V-N248D-E271F, T022A-S101G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D-H249R，T022A-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-P129E-A158E-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-P129E-A158E-G15·9E-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q2·45R-N248D-H249R, T022A-S101G-S103A-V104I-P129E-G159E-A232V-N238R-Q245R-N248D, T·022A-S101G-S103A-V104I-P129E-S188D-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-S128N-P129E-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-S128N-P129E-S188D-A232V-N238R-Q245R-N248D, T022A-S128N-A158E-H249R, V104L-S128N-A158E-H249R, V104L-S128N-A158E-R186H-E271F, V104L-S128N-A158E-R186H-H249R,和V104L-S128N-A158E-S188D-H249R，并且其中所述变体的总净电荷相对于迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的总净电荷为O、+1、+2、+3、+4、+5、-I、-2、-3、-4或-5，并且其中所述总净电荷是通过一种或多种选自以下的置换来获得的N43D, R45T, N62E, N76D, S101D, P129E, A158E, G159D, G159E, S166D, S188D, A230E, N18R, G·20K, G20R, T22R, S24R, N43R, G 118R, Q245R, H249R, N269R, E271F,和 E271L,并且其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所示解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号。
5.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列A016S-N062E-A15·8E-H249R-E271F, A016S-N062E-S128N-R186H-E271F，A016S-N062E-V104L-R186H-S188D-E2·7 IF, A016S-S101A-S128N-R186H, A016S-S128N-A158E-R186H，A016S-V104L-A158E-R186H-E·271F, A016S-V104L-S188D-H249R, A158E-R186H-H249R，A158E-R186H-S188D-H249R-E271F，·G020K-G023A-N116L-S188D, G020K-G023A-S024F-N062E-N116L-S188D-T213A, G020K-N043W-N062E-N116L-P239G, G020K-S024F-N043W-N062E-N116L-T213A, G020K-S024F-N062E, G023A-N043W-N062E-N116L-G118R-T213A, G023A-T038F-S078N-G100S-S212M-A215D, GlOOS-NlI·6L-A158E-T213A, G102A-S103N-S105T-A194E, H017R-T022A-N076D-S101G-S103A-V104I-G1·59D-A232V-Q245R-N248D-E271F，N043R-N076D-S101A-S103A-V104I-A158E-S166D-S 188D-A232V-Q245R-N248D-H249R-E27IF, N043R-S101A-S103A-V104I-A158E-S166D-S188D-A232V·-Q245R-N248D-H249R, N043W-N062E-N116L-S188D, N062E-A158E-H249R-E271F, N062E-A158E-R186H-S188D-H249R, N062E-L148I-G159E，N062E-N116L-S188D，N062E-R186H-H249R，N06·2E-S078N-G102A-N116L-S144R-L250I,N062E-S101A-A158E-R186H-H249R, N062E-S101A-A1·58E-S188D-H249R, N062E-S101A-R186H, N062E-S10IA-Rl86H-E27IF, N062E-S101A-R186H-H·249R-E271F, N062E-S101A-R186H-S188D-E271F，N062E-S101A-R186H-S188D-H249R, N062E-S101A-V104L-R186H-S188D-E271F, N062E-S128N-A158E，N062E-S128N-G159E-H249R, N062E
6.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列G118R-Y209A，G118R-Y209A-N243F, L021M-S024R-T033S, P005S-S078R-G118R-W241R, P086W-G118R-A133V, P086W-G118R-N243F, P086W-G118R-Y209A, P086W-Y209A-N243F, S024R-A174T, S024R-G118R, SO24R-G118R-Y209A, S024R-G118R-Y209A-K235F, S024R-G118R-Y209A-N243F, S024R-K235F, S024R-P086R, S024R-P086S-S141G, S024R-P086W, S024R-P086W-G118R, S024R-P086W-G118R-V203I, S024R-P086W-N243F, S024R-P086W-Y209A, S024R-S078R-P086W, S024R-S078R-P086W-G118R-A270T, S024R-S078R-P086W-N243F, S024R-T033S-A133V, S024R-T033S-G118R, S024R-T033S-P086S-S085N-K235F, S024R-T033S-P086S-S087N-Y209A, S024R-T033S-P086W, S024R-T033S-P086W-G118R, S024R-T033S-S078R-P086W, S024R-T033S-S078R-P086W-G118R, SO24R-T033S-S078R-Y209A, S024R-T033S-W241R, S024R-T033S-Y209A-K235F, S024R-Y209A-W241R, S078R-G118R, S078R-P086W-K235F, S078R-P086W-N243F, S078R-P086W-Y209A, S078R-Y209A-K235F, T033S-A172V-Y209A, T033S-G118R, T033S-G118R-G159D-Y209A, T033S-G118R-N243F, T033S-G118R-W241R, T033S-G118R-Y209A-N243F, T033S-K235F, T033S-P086W-G118R, T033S-P086W-G118R-Y209A-N243F, T033S-P086W-N243F, T033S-S078R-P086W, T033S-S078R-P086W-G118R-Y209A, T033S-S078R-Y209A, T033S-Y209A-N243F, Y209A-W241R, A001R-S009A-N043R, AOO1R-S101G-S103A-V104I-A232V-Q245R, AOO1T-N018R-S024R-N076D-N116A-T213A-H249R, A194F-G211Q-Q236N, E089I-N116A-N117F-T224A-H249R, G020R-G025R-N116A-Y167W, G020R-H249R-N269R, G020R-N043D-N076D-A230E-H249R, G020R-N043D-S078R-S101G-S103A-V104I-A232V-Q245R, G020R-N043D-S101G-S103A-V104I-A232V-Q245R, G020R-N043D-S101G-S103A-V104I-A232V-Q245R-N269R, G020R-N043R-A230E, G020R-N043R-A230E-S242R, G020R-N043R-E271L, G020R-N043R-H249R, G020R-N043R-H249R-E271L, G020R-N043R-N269R, G020R-N043R-R045T-A230E, G020R-N043R-R045T-S101A-N269R, G020R-N043R-R045T-S242R, G020R-N043R-SIOIA-Nl16A-T213A-A215F, G020R-N043R-S101A-N269R, G020R-N043R-S101G-S103A-V104I-A232V-Q245R, G020R-N043R-S212F, G020R-N043R-S212F-W241R, G020R-N043R-S242R, G020R-N043R-S242R-E271L, G020R-N043R-S242R-H249R, G020R-N043R-V244R, G020R-N043R-W241R, G020R-N062Q-E089I-R186K-S212M, G020R-N076D, G020R-N076D-S101G-S 103A-V104I-A232V-Q245R, G020R-N076D-S 101G-S 103A-V104I-A232V-Q245R-N269R, G020R-N116A-N269R, G020R-P052N-N062Q-Y091F-Y192W, G020R-Q059A-S144R-Y192W-T224A, G020R-S024R-K27E-N043R-N076D-A230E, G020R-S024R-N043D-H249R, G020R-S024R-N043D-N076D-S078R-S101G-S103A-V104I-A232V-Q245R, G020R-S024R-N043D-S242R, G020R-S024R-N043R-R045T, G020R-S024R-N043R-R045T-H249R, G020R-S024R-N043R-R045T-N116A,G020R-S024R-N043R-R045T-N116A-T213A, G020R-S024R-N043R-R045T-S101A-N269R, G020R
7.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列A016S-S128N-R186H-E271F, N076D-S101G-S103A-V104I-A158E-S188D-M222S-A232V-Q245R-N248D-H249R,S024R-S101G-S103A-V104I-S128L-P129Q-A158E-N183D-S188D-A232V-Q245R-N248D-H249R，S024R-S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R-E271G, S024R-S101G-S103A-V104I-S128L-P129Q-S130A-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129Q-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, TO 22A-N076D-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-H249R-E271F, T022A-S101G-G 102A-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F, A016S-A158E-H249R，A016S-A158E-H249R-E271F, A016S-A158E-R186H-H249R，A016S-L111V-S 188D-, A016S-T022A-A15 8E-R186H-E271F, A048R-S078N-N116L-N185E-L217E-P239G，A048R-S128D-N185E-P239G, A1 58E-R186H-H249R-E271F, E089P-S101A-P129E-R186H, G020K-G023A-N043W-N116L-S188D-T 213A-P239G, G020K-G023A-N062E-N116L-G118R-T213A, G020K-G023A-S024F-N062E-S188D-T213A-P239G, G020K-K027R-P129E-S166D-P239G, G020K-S024F-N062E-P239G, G020K-S024F -T033S-P129E-A194E, G023A-G100S-A194E-S212M，G023A-N043W-N116L-G118R-S188D, G023 A-S024F-G118R-S188D-P239G, G023A-S024F-K027R-N062E，G023A-S024F-N043W-N062E-N1 16L-G118R, G023A-S024F-N116L-G118R-S188D-T213A, G023A-S024F-N116L-S188D-P239G, K 027R-G100S-G118R-S160D-S188D-N243F, K027R-S101D-S103N-S105T-A272F, N043R-N076D-S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R, N062E-S078N-N116L -T224A, N062E-V104L-A158E-R186H-S188D-H249R, N076D-S101A-S103A-V104I-A158E-S188 D-L217E-A232V-Q245R-N248D-H249R-E27IF, N076D-S101G-S103A-V104I-A158E-S166D-S18 8D-A232V-Q245R-N248D-H249R-E271F, S024F-G102A-R186K-T213A-L217E-N243F，S024F-N0 43W-G118R-S188D, S024F-N043W-V104L-V121F-P129E, S024F-N116L-G118R-S188D-P239G, S 024F-S103N-V104L-G118R-S188D, S024R-N043R-S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-N043R-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R -N248D-H249R, S024R-N076D-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249 R-E271F, S024R-S101G-S103A-V104I-A158E-G159E-A232V-Q245R-N248D, S024R-S101G-S10 3A-V104I-S128N-A158E-G159E-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-S1 28N-P129E-S188D-A232V-Q245R-N248D, S101A-L111V-P129E, S101A-S103A-V104L-G159E-A 232V-Q245R-N248D-H249R, S101A-S103S-V104L-G159E-A232V-Q245R-N248D-H249R, S101A-V104L-S128N-A158E-R186H, S101G-S103A-V104I-P129E-L148I-S188D-A232V-Q245R-N248D ，S101G-S103A-V104I-S128N-P129E-A158E-A232V-N238R-Q245R-N248D, S101G-S103A-V104 I-S128N-P129E-S188D-A232V-Q245R-N248D-H249R, S101G-S103S-V104L-G159E-A232V-Q24 5R-N248D-H249R, S101S-S103A-V104L-G159E-A232V-Q245R-N248D-H249R, S128N-P129E-R1 86H, T022A-A158E-R186H-H249R-E271F, T022A-S024K-S101G-S103A-V104I-S128N-A158E-G 159E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-S128N-P129E-A158E-S188D -A232V-Q245R-N248D-H249R, T022A-S024R-S103A-V104I-S128I-G159D-S188D-A232V-N24 8D,T022A-S101G-S103A-V104I-L148I-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V 104I-S128N-G159E-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-S128N-P129E-A158E -A232V-N238R-Q245R-N248D, T022A-S128N-A158E-R186H, T022A-S128N-R186H-S188D-, TO 22A-V104L-A158E-H249R, T022A-V104L-R186H-S188D-H249R, T022L-G023A-K027R-S101D-V104L-S216F, T022L-S078N-N116L-P129E-S256N, T022L-S078N-S128D-T213A，T033S-G118 R-P129E-A194E-P239G, T033S-S105T-S188D-S216F, V026F-V104L-S256N-A272F, T022A-N0 43R-S 103A-V104I-S128I-G159D-S188D-A232V-Q245R-N248D, T022A-S024R-N043R-S103A-V104I-G118R-G159D-S188D-L217D-A232V-N248D, T022A-S101D-S103A-V104I-G118R-G159D-S188D-L217D-A232V-Q245R-N248D-E271F, T022A-S103A-V104I-G118R-P129E-G159D-S188D-A232V-Q245R-N248D-E271F, T022A-S103A-V104I-G159D-S188D-L217D-A232V-Q245R-N248D-E271F, T022A-S103A-V104I-S128I-P129E-G159D-S188D-A232V-N248D-E271F, T022A-S024R-S101G-S103A-V104I-P129E-L148I-A158E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-L148I-A158E-S188D-A232V-Q245R-N248D, S101G-S103A-V104I-L148I-G159E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D, S101G-S103A-V104I-S128N-P129E-A158E-A232V-Q245R-N248D-H249R, V104L-L148I-S188D-H249R, S024R-S101A-S103A-V104I-A158E-S166D-S188D-L217E-A232V-Q245R-N248D-H249R, N076D-S101G-S103A-V104I-S128L-A158E-S188D-A232V-Q245R-N248D-H249R-E271F, N043R-N076D-S101G-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, T022A-N043R-N076D-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-H249R-E271F,S101A-S103G-V104V-G159E-A232V-Q245R-N248D-H249R, S101S-S103A-V104V-G159E-A232V-Q245R-N248D-H249R, G023A-S024F-V051W-A158E, K027R-T038F-G102A-N116L, N062E-S078N-S144R-S212M, L031F-N116L-S256N-A272F，T022L-T033S-V104L-N116L-S160D-R186K, SO24F-G118R-P129E-R186K-T213A, N043W-S105T-T213A-A215D-S216F, L031F-S105T-R186K-S188D, V026F-A194E-T213A-S256N, S103N-S160D-L250I-S256N，S024R-S101G-S103A-V104I-S130A-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129Q-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, T022A-N062E-S103A-V104I-G118R-G159D-S188D-A232V-Q245R-N248D, T022A-N043R-S101D-S103A-V104I-G118R-P129E-G159D-S188D-L217D-A232V, T022A-S024R-S103A-V104I-G159D-S188D-L217D-A232V-N248D, T022A-S024R-N043R-S 103A-V104I-G118R-S128I-P129E-G159D-S188D-L217D-A232V-N248D-E271F, T022A-S103A-V104I-G118R-G159D-S188D-L217D-A232V-Q245R-N248D-E271F, G020K-N043W-S 188D-T213A, S024F-N062E-G118R-P239G, G023A-N043W-S188D-T213A, G020K-S024F-N043W-N062E-N116L-G118R-S188D-P239G, G020K-N116L-S188D-P239G，G020K-N043W-N062E-G118R, G020K-N043W-N116L-S188D-T213A, G020K-S024F，G023A-N043W-N116L-P239G, G023A-S024F-N043W-N116L-G118R-S 188D-P239G, G020K-G023A-N043W-T213A,和 G023A-S024F-N062E-G118R-T213A-P239G，并且其中所述变体的总净电荷相对于迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的总净电荷为O、+1、+2、+3、+4、+5、-I、-2、-3、_4或-5，并且其中所述总净电荷是通过一种或多种选自以下的置换来获得的N43D，R45T, N62E, N76D, SlOID, P129E, A158E, G159D, G159E, S166D, S188D, A230E, N18R, G20K, G20R, T22R, S24R, N43R, G118R, Q245R, H249R, N269R, E271F，和E271L，并且其中所述蛋白酶变体的氨基酸位置根据SEQ IDNO: I所不解淀粉芽抱杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号。
8.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I-A232V-Q236H-Q245R-N252K, S101G-S103A-V104I-A232V-Q245R-N248R, S101G-S103A-V104I-G159R-A232V-Q245R-N248D, A232V-Q245R, E089I-N117F-N185I-A215F-L233C，G020R-E0C C Crr Ccccc c· L C C KKCCC < L· L _■ C < < C _■ C rr Crrcc cccl-iv vcl-l-iv h C _■ K K Vccl-C Vl-Cl-C Cctcc&zsx-HOSc)Kss-ivzsv-loosI-ISU-VCO2S-VT2S-H8ZOS-MZSX-HOSC)-I 寸 OU-VCO2S-VT2S-H8ZOS-MZSX-HOSC)K63H-JK 艺 S-HOSOK3s-i\zsv-ISU-vco2S-HOSOrH^s-i\zsv-5ns-aco8T〒ISU-vco2s-c)T2s-H0sc)KLO艺5-i\zsv-5ns-v9n〒ISU-vco2s-c)T2s-H0sc)K^s-i\zsv-v9n〒ISU-vco2s-c)T2s-H0sc)Κ^δ-ΛZsv-Λ 寸 OU-OCOOTS-VTOTS-HOSOKLO寸s-i\zsv-feTZV-vcos-I 寸 OU-VSTS-VTOTS-HOS0.HS寸s-i\zsv-vcos-aco8T〒v9n〒ISU-vco2s-VT2s-H0sc)KLO寸s-i\zsv-feTZV-vnzx-mTS-aco8TN-v9n〒ISU-vco2s-VT2s-H0sc)K3s-i\zsv-v08u-ISU-vco2s-c)T2S-HOOZOS-HOSOOUS-HLO寸s-i\zsv-vcos-5ns-aco8T〒ISU-vco2s-c)T2s-H8zos-HOZ00K3s-i\zsv-vcos-aco8T〒v9n〒ISU-vco2s-c)T2s-H8zos-HOSc)Kss-ivzsv-feTZV-vnzx-5ns-aco8T〒v9n〒ISU-vco2s-c)T2s-H8zos-HOSc)^VZSVIfeT zv-vcos-o·ns-aco8T〒v9n〒ISU-vco2s-c)T2s-H8zos-HOSc)κ9^σ·-Λζεζν-νε ζχ-αε8 〒Λ ε ν-V9n〒lsu-Vco2s-C)T2S-H8zos-Hosc)KLO寸s-i\zsv-feTZV-aco8T〒ISU-vco2s-VT2s-HOOZOS-HOSOKLO-^s-i\zsv-feTZV-186H-aco8T〒v9n〒ISU-vco2s-VT2s-H8zos-HOSOKLO寸s-i\zsv-vcos-5ns-aco8T〒v9n〒ISU-vco2s-VT2s-H8zos-HOSc)Κ^δ-Λζεzv-feTZV-vnzx-5ns-aco8T〒v9n〒ISU-vco2s-VT2s-H8zos-HOSc)KLO寸s-i\zsv-2TS-aco8T〒v9n〒ISU-vco2s-VT2s-H8zos-HOSc)yLO寸s-i\zsv-aco8T〒v9n〒ISU-vCOOTS-VTOTS-HO OZOS-HOSOKLO寸s-i\zsv-vnzx-5ns-ISU-vco2s-VT2s-H8zos-HOSc).HS寸s-i\zsv-ISU-vco2s-VT2s-H8zos-HOSc)K6gH-JK 艺 s-a9ZONIX 等 §-HcoSN-I8cox-H 寸 SS-HOSOK3s-i\zsv-ISU-vco2s-c)T2s-a9z0N-H^0s-H0sc)&:9 ζν-νΠΖΧ-ν9π 艺-XLO寸 §-HcoSN-H^0s-H0sc)K6gH-JK 艺 s-a9zo〒xLOSH-HcoSN-H^OS-HOSc)txrLOT Zv-XssH-HCOSN-H艺0S-H0SOQ9ZON-HCOSN-H艺0S-H0SOK6SN-H9艺5-i\zsv-ISU-vco2s-c)T0TS-H8zos-a9ZON-acoSN-H^OS-HOSc)y6gH-Hgzs-acoSN-HZS-与 Ss-HosoeLOTZV-H 艺0S-H0SOy3s-i\zsv-ISU-vco2s-c)T2s-xLOSH-H0sc)K69§-v9n〒xLOSH-HOSoC6艺 H-HLO艺5-i\zsv-ISU-vco2s-c)T2s-a9z0N-x^§-H0sc)yLO艺5-i\zsv-ISU-vco2s-3T2s-a9zo〒xLOSH-HOSoK69§-feTZV-v9n〒H0soC6SN-HLO艺5-i\zsv-ISU-vco2s-oT2s-a9ZON-HcoSN-HOSc)K6^H-30sv-a9ZON-HcoSN-HOSc)K6^H-HLO艺5-i\zsv-ISU-VSTS-oTOTS-acoSN-HOSc)K3s-i\zsv-ISU-vco2s-c)T2s-H8zos-xLOSH-acoSN-HOS0K6^H-JK 艺 s-a9ZONIX 等 §-acoSN-HOSodcosl-MSU-lsu-vcoso-HOSoMz 3-168X SI/ZI 『ζ-< 8〇ζεε620 Zo
9.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列A015T-T033S，A016T-N043R-R045T-N076D-S101G-S 103A-V104I-A232V-Q245R-N269R, A230E-H249R, A232V-H249R, E089I-S105T-N116A-A215F-S216F, E089I-Y091F-N185I-G211Q-A270C, G020R-A090S-S101G-S103A-V104I-N116A-N183D-T213A-A215F-A232V-Q245R, G020R-N043D-S078R-S10IG-S103A-V104I-A232V-Q245R-H249R, G020R-N043R-N076D, G020R-N043R-R045T-S078R-S101G-S103A-V104I-A232V-Q245R, G020R-P052N-S101A-1198L-L233C, G020R-R045T-N076D-S078R-S101G-S103A-V104I-A232V-Q245R-N269R, G020R-S024R-N043D-N076D-H249R, G020R-S024R-N043D-N076D-S242R-H249R, G020R-S024R-N043D-R045T-A230E-S242R, G020R-S024R-N043D-S078R-S101G-S103A-V104I-A232V-Q245R, G020R-S024R-N043D-S078R-S101G-S103A-V104I-A232V-Q245R-H249R, G020R-S024R-N043R-S101G-S103A-V104I-A232V-Q245R, G020R-S024R-N076D-S078R-S101G-S103A-V104I-A232V-Q245R-H249R, G020R-S024R-R045T-N076D-S101G-S103A-V104I-A232V-Q245R, G020R-S024R-R045T-N076D-S242R-H249R, G020R-S024R-R045T-S101G-S103A-V104I-A232V-Q245R, G020R-S078R-S101A-S103A-V104I-G115E-N116A-N183D-G211Q-T213A-A232V-Q245R, G020R-S101A-S103A-V104I-N116A-N183D-A215F-A232V-Q245R, G020R-S101A-S103A-V104I-N116A-N183D-A232V-Q245R, G020R-S101A-S103A-V104I-N116A-N183D-G211Q-A232V-Q245R, GO20R-SIOIA-SIO3A-VIO41-N116A-N183D-G211Q-A232V-Q245R-T274I,G020R-S101A-S103A-V104I-N116A-N183D-I198L-G211Q-T213A-A215F-A232V-Q245R, G020R-S101A-S103A-V104I-N116A-N183D-T213A-A215F-A232V-Q245R, G020R-S101A-S103A-V104I-T213A-A232V-Q245R, G020R-S101G-S103A-V104I-N116A-N183D-A232V-Q245R, G020R-S101G-S103A-V104I-N116A-N183D-G211Q-A215F-A232V-Q245R, G020R-S101G-S103A-V104I-N116A-N183D-G211Q-A232V-Q245R, G020R-S101G-S103A-V104I-N116A-N183D-G211Q-T213A-A215F-A232V-Q245R, G020R-S101G-S103A-V104I-N116A-N183D-G211Q-T213A-A232V-Q245R, G020R-S101G-S103A-V104I-N116A-N183D-T213A-A232V-Q245R, G020R-T022R-N269R, G020R-T022R-S078R-S212F-W241R, G020R-T022W-S101A-S103A-V104I-N116A-N183D-A215F-A232V-Q245R, G020R-T022W-S101A-S103A-V104I-N116A-N183D-A232V-Q245R-N269S, G020R-T022ff-S101A-S103A-V104I-N116A-N183D-A232V-Q245R-T274I,G020R-T022W-S101A-S103A-V104I-N116A-N183D-G211Q-A215F-A232V-Q245R, G020R-T022W-S101A-S103A-V104I-N116A-N183D-I198L-G211Q-T213A-A215F-A232V-Q245R, G020R-T022W-S101A-S103A-V104I-N116A-N183D-T213A-A232V-Q245R, G020R-T022W-S101A-S103A-V104I-N183D-G211Q-T213A-A215F-A232V-Q245R, G020R-T022W-S101A-S103A-V104I-N183D-I198L-A215F-A232V-Q245R, G020R-T022W-S101A-S103A-V104I-N183D-T213A-A215F-A232V-Q245R, G020R-T022W-S101G-S103A-V104I-N116A-N183D-A232V-Q245R, G020R-T022W-S10IG-S103A-VI04I-N116A-N183D-G211Q-A232V-Q245R-N263S, G020R-T022W-S101G-S103A-V104I-N116A-N183D-G211Q-T213A-A215F-A232V-Q245R, G020R-T022W-S101G-S103A-V104I-N116A-N183D-G211Q-T213A-A232V-Q245R, G020R-T022W-S101G-S103A-V104I-N183D-A215F-A232V-Q245R,G020R-T022W-S101G-S103A-V104I-N183D-A232V-Q245R, G025R-N062Q-S128N-S144R-N185.1,G025R-N116A-H120F-T224A-A270C, G118R-K235F, K027R-N043R-R045T-N076D-S101G-S10.3A-V104I-A232V-Q245R, N018K-N076D-S078R-S101G-S103A-V104I-L217E-A232V-Q245R-N2
10.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X-43R, X020K-X062E，X024F-X 116L, X020K-X024F，X024R-X174T, X024R-X118R, X024R-X235F, X024R-X086R, X024R-X086W, X078R-X118R, X033S-X118R, X033S-X235F, X209A-X24IR, X020R-X076D, X018R-X245R, X024R-X045T, X232V-X245R, X118R-X172V, X118R-X194T, XO08T-X024R, X235F-X243F, X018R-X103A, X018R-X104I, X086W-X118R, X086W-X243F, X086W-X209A, X024C-X033S, X024R-X232V, X024R-X243F, X024R-X239Q, X024R-X101G, X024R-X141G,X024R-X033S, X024R-X274I, X024R-X209A, X078R-X086W, X101G-X232V, X033S-X148F, X033S-X086W, X033S-X201S, X033S-X078R, X033S-X241R, X033S-X209A, X230E-X249R, X232V-X249R, X118R-X235F, X076D-X245R, X086W-X235F, X024R-X247H, X024R-X104A, X078R-X235F, XlO1G-X249R, X103A-X232V, X033S-X048T, X033S-X239T, X033S-X253A, X143A-X209A, X209A-X235F, X018R-X045T, X209A-X243F, X024R-X272P, X024R-X269C, X101G-X104I，X104I-X232V, X076D-X249R，和X024R-X076D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
11.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X076D，X024R-X045T, X230E-X249R, X018R-X045T, X018R-X245R, X101G-X232V, X024R-X232V,X232V-X245R, X024R-X101G, X018R-X104I, X018R-X103A, X101G-X249R, X232V-X249R, X103A-X232V,X076D-X245R, X101G-X104I，X104I-X232V, X076D-X249R, X024R-X076D, X024F-X116L，X020K-X024F, X020K-X062E，X033S-X118R, X024R-X086W, X024R-X118R, X024R-X086R, X209A-X241R, X024R-X241R, X024R-X235F, X118R-X209A, X078R-X118R, X033S-X235F, X024R-X174T, X086W-X209A, X008T-X024R，X086W-X118R, X033S-X241R, X024R-X243F, X024R-X209A, Χ033S-X086W, X024R-X033S, X086W-X243F, X033S-X201S, X024R-X239Q, X078R-X086W, X235F-X243F, X118R-X172V, X033S-X148F, X033S-X078R, X033S-X243F, X024C-X033S, X118R-X194T, X033S-X209A, X024R-X141G, X024R-X274I, X086W-X235F, X015T-X033S, X209A-X235F, X024R-Χ247Η, X078R-X235F, X024R-X104A, X033S-X048T, X118R-X235F, X033S-X253A, Χ143Α-Χ209Α，X033S-X239T, X209A-X243F, X024R-X272P，和 X024R-X269C，其中所述枯草杆菌蛋白酶变体的氨基 酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
12.—种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X004R-X009A-X020R-X242R, X020R-X043R-X241R, X020R-X242R-X269R, X004R-X009A-X020R-X043R, X004R-X020R-X249R, X018R-X024R-X244R, X009A-X022R-X212F-X241R, X020R-X043R-X269R, XOl8R-X024R-X242R, X004R-X009A-X043R-X241R, X020R-X043R-X244R, X020R-X022R-X242R, X004R-X020R-X043R, X004R-X009A-X020R-X043R-X242R, X020R-X043R-X242R, X020R-X043R-X242R-X249R, X020R-X212F-X249R, X004R-X009A-X241R, X001R-X009A-X043R, X020R-X043R-X249R, X009A-X020R-X043R-X241R, X020R-X022R-X043R, X020R-X249R-X269R, X020R-X022R-X241R, X004R-X009A-X024R-X043R-X241R, X009A-X043R-X078R, X004R-X020R-X024R-X244R, X020R-X022R-X078R-X242R, X020R-X024R-X242R-X249R, X004R-X009A-X078R-X24IR, X009A-X043R-X078R-X242R, X004R-X020R-X024R, X009A-X043R-X212F, X020R-X043R-X212F, X024R-X078R-X212F, X009A-X020R-X024R-X043R, X009A-X022R-X043R-X078R, X020R-X022R-X212F-X241R, X020R-X043R-X212F-X241R, X009A-X043R-X241R, X020R-X043R-X271L, X020R-X022R-X078R-X241R, X020R-X024R-X043R-X242R, X020R-X022R-X043R-X241R, XO09A-X020R-X043R-X212F, X004R-X009A-X020R-X024R-X242R, X020R-X043R-X249R-X271L,X020R-X022R-X024R-X242R, X009A-X022R-X078R-X212F, X020R-X043R-X242R-X271L, XOO9A-X022R-X078R-X212F-X241R, X004R-X020R-X024R-X249R, X020R-X022R-X271L, X020R-X022R-X043R-X212F, X004R-X020R-X024R-X043R-X242R, X004R-X020R-X024R-X043R, X004R-X009A-X022R-X078R-X212F, X020R-X022R-X078R-X212F-X241R,和 X020R-X022R-X269R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
13.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X018R-X020R-X043D-X045T-X230E, X018R-X043R-X045T-X242R-X249R, X024R-X043D-X249R, X018R-X020R-X045T, X020R-X024R-X076D-X249R, X024R-X043R-X230E-X242R, X018R-X024R-X043D-X230E, X020R-X076D, X018R-X024R-X043D-X076D-X249R, X024R-X043R-X076D-X249R, XO18R-X024R-X045T-X242R, X020R-X043D-X076D-X230E-X249R,X020R-X043R-X045T-X242R, X018R-X024R-X076D-X249R, X018R-X020R-X024R-X043D-X045T-X233I-X242R, X024R-X043R-X230E, X018R-X020R-X043D, X043R-X242R-X249R, X020R-X043R-X045T-X230E, XO
14.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101G-X103A-X104I-X232V-X236H-X245R-X252K, X101G-X103A-X104I-X232V-X245R-X248R, X101G-X103A-X104I-X159R-X232V-X245R-X248D,X101G-X103A-X104I-X159D-X232V-X245R-X248R, XlOlG-X103A-X104I-X232V-X245R, X101G-X103A-X104I-X159D-X232V-X245R 和 X101G-X103A-X104I-X232V-X245R-X248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
15.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101G-X103A-X104I-X129E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D-X249R,X024R-X101G-X103A-X104I-X129E-X159E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128N-X129E-X158E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X148I-X158E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X159E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128N-X129E-X232V-X245R-X248D, X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X158E-X159E-X188D-X232V-X245R-X248D-X249R, XO22A-X024R-X101G-X103A-X104I-X129E-X158E-X159E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X148I-X158E-X232V-X245R-X248D, X016S-X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R,X101G-X103A-X104I-X158E-X159E-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X129E-X158E-X159E-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X159E-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X128N-X158E-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X158E-X188D-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X128N-X129E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X158E-X159E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X128N-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X158E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X1041-X128N-X129E-XI58E-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X159E-X188D-X232V-X245R-X248D, X022A-X101G-X103A-X104I-X128N-X129E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X159E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158EC _■ C _■ c· L C K Kc C C tc—ll-yv h C τ·7ν h -' C _■ Ce _■ C _■ C _■ c· -' C < C C Ce -' Ce -' C-Η93χ-ΛΖ Χ-389 χ-Ι#ο χ-νεο χ-ο 2χ-等 Sx-VzsxΩ83χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 X-I 寸ο χ-νεο χ-ο ο χQOC艺 X-HLO艺 χ-ΛΖ Χ-369 χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-Η 艺Ox-VzsxQ8 艺 χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 χ-389 χ-362χ-Ι40 χ-νε2χ-ο 2χ^63χ-α8艺X-HLO艺 χ-ΛΖ Χ-369 χ-389 χ-36ζ χ-Ι#2χ-νε2χ-ο 2χ-νζδχΩ8 艺 Χ-Η93Χ-Η8 Χ-Λzsx-sLOT—ix-sLOT—ix-IST—ix-vcooT—ix-oT—ioT—ix-等 SxQoc艺 χ-Η93χ-Η8 Χ-ΛΖ Χ-369 χ-389 χ-I 寸oT—ix-vcooT—ix-oT—ioT—ix-vzsx C6^x-a0c艺 X-HLO艺 χ-ΛΖ Χ-389 χ-Ι42χ-νε2χ-ο 2χ-筇 SxΩ83χ-Η93χ-ΛΖ Χ-α88 χ-Ι8^χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-等 Sx C6^x-a8 艺 x-HLOgχ-ΛΖ Χ-α88 χ-369 χ-362χ-Ι#ο χ-νεο χ-ο ο χ-νζζοχQOC艺 x-HLOgx-H8sx-$sx-38LOTX-ISTX-VSTX-oTOTXQ83x-HLO3x-H8sx-i^sx-a88TX-36LOTX-36^x-ISTX-vcoOTX-oTο χ δχ^6^x-aoc艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-369 χ-Ι#ο χ-νεο χ-ο ο χ-νζδχQOC艺 X-HLo艺χ-ΛΖ Χ-α88 χ-369 χ-Ν8ζ χ-Ι42χ-νε2χ-ο 2χ-等 SxQ8 艺 X-HLO艺 X-H8SX-$SX-369 χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο ο χ-等 Sx C6^x-a8 艺 X-HLO艺 χ-ΛΖ Χ-369 χ-389 χ-36ζ χ-Ι#2χ-νεο χ-ο ο χ-等 SxΩ8$χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 χ-369 χ-Ι#2χ-νε2χ-ο ο χ-等 Sx C6^x-a8 艺 χ-Η93χ-ΛΖ Χ-369 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-νζδχQ8 寸 zx-hlo艺 χ-ΛΖ Χ-α88 χ-389 χ-Ι8^χ-36ζ χ-Ι#2χ-νεο χ-ο ο χ-等 Sx C6^x-a8 艺 X-HLO艺χ-ΛΖ Χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-νζδχ C6^x-a8 艺 X-HLO艺 χ-ΛΖ Χ-369 χ-36ζ χ-Ι42χ-νε2χ-ο 2χΩ8 艺 χ-Η93χ-Η8 Χ-ΛΖ Χ-369 χ-36ζ χ-Ι40 χ-νεο χ-ο ο χ-VzsxQOC艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-36ζ χ-Ν8ζ χ-Ι42χ-νε2χ-ο 2χ-等 Sx-VzsxQocgx-Η93χ-ΛΖ Χ-389 χ-Ν8^χ-Ι^ χ-νε2χ-ο 2χ δχ C6^x-a8 艺 X-HLO艺 χ-ΛΖ Χ-α88 -χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χΩ8 艺 χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 χ-369 χ-389 χ-Ι 寸ο χ-νεο χ-ο ο χ-等 Sx-Vzsx C6^x-a8 艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-369 χ-389 χ-36ζ χ-Ι寸ο χ-νεο χ-ο ο χ-等 Sxdocgx-HLO艺 χ-Η8 Χ-ΛΖ Χ-α88 χ-36ζ χ-Ι42χ-νεο χ-ο ο χ-VzsxQOC艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-389 χ-Ι40 χ-νε2χ-ο 2χ-νζδχ y6gx-aocgx-HLOgx-ΛΖ Χ-α88 χ-369 χ-389 χ-Ι42χ-νε2χ-ο 2χ-νζδχQocgx-HLO艺 χ-ΛΖ Χ-389 χ-Ι8 寸 Ix-IST—ix-vcooT—ix-oT—ioT—ix-等 Sx C6^x-a8 艺 X-HLO艺 χ-ΛΖ Χ-389 χ-36ζ χ-Ι#2χ-νεο χ-ο οTX 丨等 Sx-VzsxQOC艺 χ-Η93χ-Η8 Χ-ΛΖ Χ-389 χ-36ζ χ-Ι42χ-νε2χ-ο 2χ-νζδχ C6艺χ-αοο艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-369 χ-Ι#2χ-νεο χ-ο ο χ-等 SxQOC艺 X-HLO艺 χ-ΛΖ Χ-α88 χ-369 χ-389 χ-Ι42χ-νε2χ-ο ο χ-等 Sx-VzsxΩ83χ-Η93χ-Η8 Χ-ΛΖ Χ-α88 χ-X STAC f ^ ^TuKl V SOZCOCOSOT—Iδ159E-X188D-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X129E-X148I-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X232V-X238R-X245R-X248D, X101G-X103A-X104I-X128N-X129E-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X128N-X159E-X232V-X245R-X248D,X022A-X101G-X103A-X104I-X128N-X129E-X158E-X232V-X238R-X245R-X248D, XlOlG-Xl03A-X104I-X128N-X129E-X188D-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X128N-X129E-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128N-X158E-X159E-X188D-X232V-X245R-X248D, X022A-X024K-X101G-X103A-X1041-X128N-X 158E-X159E-X232V-X245R-X248D, X101G-X103A-X104I-X129E-X148I-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X148I-X232V-X245R-X248D, X022A-X101G-X103A-X104I-X148I-X188D-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X128N-X129E-X188D-X232V-X245R-X248D, X101G-X103A-X104I-X128N-X129E-X158E-X232V-X238R-X245R-X248D,X022A-X024R-X101G-X103A-X104I-X129E-X148I-X158E-X188D-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X148I-X158E-X188D-X232V-X245R-X248D, X101G-X103A-X104I-X148I-X159E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D,和 X101G-X103A-X104I-X128N-X129E-X158E-X232V-X245R-X248D-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
16.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X022A-X024R-X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X129E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X158E-X159E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X159E-X188D-X232V-X245R-X248D-X249R, X022A-X024R-X101G-X103A-X104I-X158E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X159E-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X232V-X245R-X248D,X024R-X101G-X103A-X104I-X148I-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X148I-X158E-X232V-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D-X249R, X016S-X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129E-X159E-X232V-X238R-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X245R-X248D-X249R, X022A-X101G-X103A-X104I-X129E-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X148I-X158E-X232V-X245R-X248D, X024R-X101G-X103A-X104I-X129E-X188D-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X158E-X159E-X188D-X232V-X238R-X245R-X248D, X022A-X101G-X103A-X104I-X158E-X159E-X232V-X238R-X245R-X248D, X022A-X024R-X101G-X103A-X104I-X129E-X158E-X232V-X245R-X248D, X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R,X022A-X101G-X103A-X104I-X158E-X159E-X232V-X245R-X248D-X249R，和 X024R-X101G-X103A-X104I-X158E-X159E-X232V-X245R-X248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
17.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X104L-X128N-X158E-X186H-X249R, X128N-X158E-X188D-X249R, X062E-X128N-X158E-X159E-X271F，X062E-X158E-X188D-X249R-X271F, X062E-X158E-X186H-X249R-X271F，X128N-X158E-X188D-X209E-X271F, X062E-X159E-X188D-X249R, X016S-X062E-X158E-X186H-X249R, X062E-X158E-X159E-X249R, X101A-X128N-X158E-X209E-X249R, X128N-X158E-X186H-X271F，X062E-X158E-X188D-X249R, X062E-X158E-X186H-X271F, X062E-X158E-X186H-X249R, X062E-X101A-X186H-X249R, X062E-X101A-X158E-X186H-X271F, X062E-X104L-X158E-X188D-X249R-X271F，X062E-X159E-X186H-X249R, X062E-X159E-X249R，X128N-X158E-X186H-X249R，X128N-X158E-X188D-X271F, X062E-X158E-X249R, X062E-X186H-X188D-X249R-X271F，X128N-X158E-X209E-，X062E-X101A-X158E-X249R, X104L-X128N-X158E-X186H-X271F, X062E-X101A-X158E-X186H-X249R-X271F, X016S-X062E-X158E-X249R, X062E-X101A-X159E-X249R, X128N-X158E-X186H-X188D-X271F, X101A-X128N-X158E-X186H-X271F, X062E-X101A-X188D-X249R, X101A-X104L-X158E-X186H-X188D-X249R, X062E-X159E-X249R-X271F，X128N-X158E-X159E-X271F，X016S-X062E-X104L-X158E-X186H-X271F, X022A-X128N-X158E-X249R, X128N-X158E-X249R, X062E-X101A-X104L-X158E-X186H-X271F, X016S-X062E-X158E-X186H-X271F，X104L-X128N-X158E-X249R, X104L-X128N-X158E-X188D-X249R, X022A-X062E-X158E，X062E-X101A-X188D-X249R-X271F, X062E-X158E-X249R-X271F, X104L-X128N-X158E-X186H-X188D-X271F，X062E-X101A-X186H-X271F, X062E-X104L-X159E-X249R, X062E-X186H-X249R, X062E-X101A-X186H-X249R-X271F, X101A-X158E-X186H-X188D-X249R，X062E-X101A-X186H，X101A-X128N-X129E-X186H-X249R, X101A-X103G-X158E-X186H-X249R, X016S-X062E-X104L-X186H-X188D-X271F,X104L-X158E-X186H-X249R, X101A-X128N-X158E-X188D-X209E-X271F, X062E-X101A-X186H-X188D-X271F, X016S-X062E-X158E-X249R-X271F, X062E-X128N-X158E，X062E-X128N-X159E-X249R, X062E-X101A-X158E-X188D-X249R, X101A-X128N-X158E-X249R, X062E-X158E-X186H-X188D-X249R, X016S-X104L-X158E-X186H-X271F, X062E-X148I-X159E，X062E-X101A-X158E-X186H-X249R, X062E-X101A-X186H-X188D-X249R, X104L-X158E-X186H-X188D-X249R, X062E-X101A-X104L-X186H-X188D-X271F, X022A-X101A-X158E-X186H-X249R，X101A-X128N-X158E-X209E, X158E-X186H-X188D-X249R-X271F, X104L-X158E-X186H-X188D-X249R-X271F，X101A-X104L-X158E-X186H-X249R, X104L-X158E-X249R, X101A-X104L-X128N-X158E-X186H-X271F, X016S-X104L-X188D-X249R, X101A-X104L-X158E-X186H-X188D-X271F，X104L-X128N-X159E-X271F, X104L-X158E-X186H-X249R-X271F，X158E-X186H-X249R，X101A-X158E-X186H-X249R, X104L-X158E-X188D-X249R-X271F，XO16S-X128N-X158E-X186H, X104L-X128N-X186H-X188D-X249R, X016S-X101A-X128N-X186H, X016S-X062E-X128N-X186H-X271F, X016S-X128N-X186H-X271F, X128N-X129E-X186H，X158E-X186H-X249R-X271F, X016S-X158E-X249R，X016S-X158E-X186H-X249R, X016S-X022A-X158E-X186H-X271F，X089P-X101A-X129E-X186H,X022A-X128N-X158E-X186H, X101A-X104L-X128N-X158E-X186H, X022A-X128N-X186H-X188D-, X062E-X104L-X158E-X186H-X188D-X249R, X022A-X158E-X186H-X249R-X271F，X022A-X104L-X158E-X249R, X101A-X111V-X129E, X016S-X158E-X249R-X271F，X016S-X111V-X188D-,X022A-X104L-X186H-X188D-X249R，和 X104L-X148I-X188D-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
18.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X001R-X101G-X103A-X104I-X232V-X245R, X004R-X101G-X103A-X104I-X232V-X245R, X043R-X101G-X103A-X104I-X232V-X245R-X271L, X078R-X101G-X103A-X104I-X232V-X245R, X004R-X043R-X101G-X103A-X104I-X232V-X245R, X018R-X043R-X101G-X103A-X104I-X232V-X245R, X020R-X101G-X103A-X104I-X232V-X245R, X101G-X103A-X104I-X232V-X245R-X271L, X020R-X043R-X101G-X103A-X104I-X232V-X245R, X024R-X043R-X101G-X103A-X104I-X232V-X245R, X020R-X025R-X116A-X167W, X018R-X101G-X103A-X104I-X232V-X245R, X022R-X101G-X103A-X104I-X232V-X245R, X078R-X103N-X106G-X167W-X236N, XO18R-X043D-X101G-X103A-X1041-X232V-X245R-X269R, X043R-X101G-X103A-X104I-X232V-X245R-X269R, X024R-X101A-X120F-X194F-X249R, X020R-X043D-X101G-X103A-X104I-X232V-X245R-X269R, X101G-X103A-X104I-X212F-X232V-X245R, X020R-X144R-X185I-X233C-X236N, X023A-X078R-X216F-X236N-X249R, X101G-X103A-X104I-X232V-X245R-X269R, X101G-X103A-X104I-X115R-X232V-X245R,X052N-X078R-XIO3N-X1481-X213A, X018R-X043D-X101G-X103A-X104I-X232V-X245R-X249R, X024R-X043D-X101G-X103A-X104I-X232V-X245R-X249R, X024R-X043D-X101G-X103A-X104I-X232V-X245R-X269R, X025R-X089I-X116A-X239S-X270C, X024R-X101G-X103A-X104I-X232V-X245R, X148I-X213A-X252R, X024R-X025R-X183D-X192W-X239S, X046R-X194F-X212M, X104L-X217E-X224A-X249R-X252R, X023A-X091F-X121F-X192W-X236N, X101G-X103A-X104I-X232V-X244R-X245R, X099F-X144R-X167W-X252R, X101G-X103A-X104I-X232V-X245R-X249R, X043R-X101G-X103A-X104I-X232V-X245R, XO22W-X078R-X167W-X212M-X270C, X121F-X252R-X270C, X020R-X103N-X216F-X236N-X252R，X043R-X101G-X103A-X104I-X232V-X245R-X249R, X023A-X052N-X192W-X198L-X252R, X025R-X046R-X121F, X024R-X078R-X104L-X116A-X183D, XO46R-X059A-XIO3N-X211Q-X212M, X020R-X052N-X062Q-X091F-X192W, X023A-X052N-X144R-X192W-X216F, X101G-X103A-X104I-X232V-X242R-X245R, X052N-X103N-X116A-X148I-X192W, X089I-X116A-X117F-X224A-X249R, X144R-X211Q-X238L-X239S-X249R, X043A-X062Q-X194F-X211Q, X020R-X024R-X052N-X059A-X216F, X024R-X167W-X224A-X249R, X057R-X167W-X249R, X025R-X103N-X186K-X194F-X224A, XIO5T-X128N-X144R-X1481-X212M, XO20R-X059A-X144R-X192W-X224A, X024R-X043A-X117F-X194F-X21IQ, Xl17F-X194F-X213A-X270C, XO78R-X09IF-X121F-X233C-X252R, X057R-X099F-X105T-X198L-X213A, X023A-X091F-X101A-X198L-X252R, X062Q-X103N-X121F-X144R-X249R, X043R-X101G-X103A-X104I-X232V-X242R-X245R, X023A-X024R-X117F-X212M-X216F, X104L-X213A-X216F, X194F-X211Q-X236N, X062Q-X103N-X117F-X194F, X024R-X062Q-X104L-X106G-X249R, X057R-X089I-X198L, XO46R-X059A-X106G-X217E-X249R, X117F-X213A-X215F, X101A-X120F-X192W-X215F-X224A,X043A-X057R-X117F-X144R-X183D, X046R-X183D-X238L，X025R-X043A-X089I-X117F，X078R-X104L-X213A-X215F-X224A, X091F-X099F-X101A-X105T-X167W, X106G-X117F-X238L，X046R-X0891-X09IF-XlOIA-Xl16A, X020R-X062Q-X0891-X186K-X212M, X057R-X099F-X121F-X185I-X192W, X046R-X089I-X192W-X233C-X270C, X089I-X117F-X185I-X215F-X233C, X052N-X104L-X183D-X216F-X249R, X078R-X099F-X116A-X186K-X224A, X025R-X105T-X128N-X144R-X270C, X105T-X211Q-X216F, X024R-X046R-X091F-X121F，X106G-X185I-X216F-X236N，X062Q-XlO1A-X236N-X252R-X270C, X025R-X043A-X091F-X198L-X270C, X020R-X023A-X104L-X192W-X233C, X024R-X043A-X105T-X106G-X198L, X020R-X089I-X217E，X024R-X091F-X198L-X215F-X239S, X046R-X089I-X099F-X186K-X212M，X104L-X120F-X186K-X216F-X252R，X022W-X194F-X213A-X233C-X238L, X099F-X105T-X106G-X194F-X212M，X089I-X105T-X116A-X215F-X216F, X025R-X116A-X120F-X224A-X270C, X043A-X059A-X101A-X216F-X224A，X057R-X183D-X236N, X025R-X062Q-X128N-X144R-X185I, X103N-X120F-X167W-X198L-X233C, X022W-X089I-X216F, X024R-X106G-X116A-X212M-X224A, X020R-X052N-X101A-X198L-X233C, X089I-X091F-X185I-X211Q-X270C, X111I-X215F-X239S, X024R-X116A-X186K-X233C-X236N,和X023A-X103N-X106G-X212M-X215F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
19.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X022W-X078R-X101A-X103A-X104I-X116S-X213A-X215F-X232V-X245R, X018R-X078R-X101G-X103A-X104I-X232V-X245R, X024R-X045T-X101G-X103A-X104I-X232V-X245R-X269R, X020R-X022W-X078R-X101G-X103A-X104I-X116A-X232V-X245R, X020R-X22W-X101G-X103A-X104I-X232V-X245R, X018R-X043R-X101G-X103A-X104I-X232V-X245R, X018R-X022W-X024R-X076D-X101A-X116A-X232V-X245R, X018R-X104I-X232V-X249R, X018R-X024R-X076D-X101A-X116A-X211Q-X249R, X018R-X043D-X078R-X101G-X103A-X104I-X217E-X232V-X245R, X018R-X043R-X101G-X103A-X104I-X232V-X245R-X269R, X018R-X045T-X076D-X101G-X103A-X104I-X232V-X245R-X269R, X076D-X078R-X101G-X103A-X104I-X232V-X245R-X269R, X020R-X043D-X078R-X101G-X103A-X104I-X232V-X245R, X018R-X043D-X076D-X101G-X103A-X104I-X232V-X245R-X269R, X024R-X045T-X076D-X078R-X101G-X103A-X104I-X232V-X245R-X269R, X018R-X103A-X232V-X249R, X018R-X101G-X104I-X232V-X245R, X020R-X024R-X101G-X103A-X104I-X217E-X232V-X245R-X249R, X018R-X22K-X043D-X101G-X103A-X104I-X232V-X245R, X043R-X045T-X101G-X103A-X104I-X232V-X245R-X269R, X020R-X22W-X101G-X103A-X104I-X211Q-X232V-X245R, X024R-X076D-X078R-X101G-X103A-X104I-X232V-X245R-X269R, X020R-X22W-X078R-X101A-X103A-X1041-X116A-X183D-X232V, XO18R-X024R-X076D-X116A-X215F-X249R, XOI8R-X043R-X045T-X101G-X103A-X104I-X232V-X245R, X024R-X043R-X076D-X101G-X103A-X104I-X232V-X245R, X020R-X022W-X101G-X103A-X104I-X232V-X245R, X020R-X022W-X101G-X103A-X104I-X211Q-X232V-X245R, XO20R-X022W-X078R-XIOIG-XIO3A-X1041-X116A-X213A-X215F-X232V-X245R, X043D-X076D-X078R-X101G-X103A-X104I-X232V-X245R-X269R, X018R-X024R-X076D-X101A-X116A-X213A-X249R, X018R-X024R-X076D-X116A-X211Q-X249R, X043R-X045T-X076D-X078R-X101G-X103A-X104I-X232V-X245R, X018R-X101G-X245R, X020R-X22
20.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X018R-X024R-X043R-X076D-X249R-X269R, X018R-X022R-X024R-X043R-X076D-X249R, X018R-X043D-X101G-X103A-X104I-X232V-X245R, X020R-X043D-X101G-X103A-X104I-X232V-X245R, X043D-X101G-X103A-X104I-X232V-X245R-X269R, X043D-X078R-X101G-X103A-X104I-X232V-X245R, X043R-X076D-X101G-X103A-X104I-X232V-X245R, X022R-X043R-X101G-X103A-X104I-X232V-X245R, X043R-X078R-X101G-X103A-X104I-X232V-X245R, X020R-X076D-X101G-X103A-X104I-X232V-X245R, X043R-X076D-X101G-X103A-X104I-X232V-X245R, X022R-X076D-X101G-X103A-X104I-X232V-X245R, X076D-X078R-X101G-X103A-X104I-X232V-X245R, X018R-X024R-X043R-X076D-X249R, XO18R-X024R-X076D-X242R-X249R, X018R-X024R-X076D-X249R-X269R, X018R-X022R-X024R-X076D-X249R, X018R-X024R-X076D-X078R-X249R, X018R-X024R-X043D-X076D-X249R-X269R, X018R-X022R-X024R-X043D-X076D-X249R, XO18R-X024R-X043D-X076D-X078R-X249R, X020R-X101G-X103G-X104I-X232V-X245R, X020R-X101G-X103A-X104L-X232V-X245R, X020R-X101G-X103A-X104V-X232V-X245R, X020R-X101G-X103S-X104I-X232V-X245R, XO20R-X101G-X103S-X104L-X232V-X245R, X020R-X101S-X103S-X104I-X232V-X245R, X020R-X101S-X103S-X104L-X232V-X245R, X020R-X101A-X103A-X104L-X232V-X245R, X020R-X101S-X103S-X104V-X232V-X245R, X020R-X101S-X103A-X104I-X232V-X245R, X020R-X101S-X103A-X104V-X232V-X245R, X020R-X101S-X103G-X104I-X232V-X245R, X020R-X101S-X103G-X104V-X232V-X245R, X020R-X101A-X103A-X104V-X232V-X245R, X020R-X101A-X103S-X104I-X232V-X245R, X020R-X101A-X103S-X104V-X232V-X245R, X018R-X024R-X043R-X076D-X078R-X2 49R, X024R-X043D-X101G-X103A-X104I-X232V-X245R, X043D-X101G-X103A-X104I-X232V-X245R-X249R, X024R-X076D-X101G-X103A-X104I-X232V-X245R, X076D-X101G-X103A-X104I-X232V-X242R-X245R, X018R-X020R-X024R-X076D-X217E-X249R, X018R-X024R-X043R-X076D-X217E-X249R, X018R-X024R-X043D-X076D-X242R-X249R, X018R-X020R-X024R-X043R-X076D-X249R, X020R-X101A-X103G-X104V-X232V-X245R, X043D-X101G-X103A-X104I-X232V-X245R, XO18R-X024R-X076D-X217E-X249R-X269R,和 X018R-X024R-X076D-X217E-X242R-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
21.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X101A-X103A-X104I-X118R-X232V-X245R, X020R-X024R-X116A-X213A, X043R-X101A-X116A-X215F-X269R, X024R-X043R-X10IA-Xl16A, X024R-X043R-X101A-X116A-X215F-X269R, X020R-X101G-X103A-X104I-X215F-X232V-X245R, X043R-X101A-X269R, X024R-X043R-X116A-X213A-X269R，X020R-X024R-X043R-X045T-X101A-X213A, X024R-X043R-X116A-X215F-X269R, X020R-X024R-X213A-X215F, X020R-X116A-X269R, X024R-X116A-X213A-X269R, X043R-X10IA-Xl16A-X269R, X101G-X103A-X104I-X116A-X213A-X232V-X245R-X269R, X024R-X043R-X045T-X101A-X116A-X215F-X269R, X020R-X043R-X101A-X269R, X101A-X103A-X104I-X213A-X232V-X245R-X269R, X024R-X215F-X269R, X043R-X101A-X116A-X213A-X215F-X269R，X043R-X101A-X213A-X269R, X020R-X024R-X043R-X045T-X116A-X213A, X101G-X103A-X104I-X232V-X245R-X269R，X024R-X043R-X045T-X101A-X116A-X213A-X269R, X024R-X043R-X045T-X269R, X020R-X043R-X045T-X101A-X269R, X024R-X043R-X116A-X269R, X020R-X024R-X043R-X045T, X043R-X116A-X269R, X024R-X043R-X101A-X215F-X269R, X024R-X043R-X045T-X213A-X215F-X269R, XO20R-X024R-X045T-X269R, X020R-X043R-X101A-X116A-X213A-X215F, X020R-X101G-X103A-X104I-X213A-X215F-X232V-X245R, X020R-X024R-X045T-X116A-X269R, X020R-X101A-X116A-X269R, X024R-X043R-X215F, X020R-X024R-X213A, X024R-X043R-X101A-X215F, X020R-X024R-X043R-X045T-X116A, X020R-X024R-X043R-X045T-X101A-X269R, X020R-X024R-X101A-X215F，X020R-X024R-X116A-X213A-X215F, X020R-X024R-X116A, X020R-X024R-X101A-X116A, X043R-X213A-X215F-X269R, X024R-X101A-X269R, X024R-X043R-X116A-X215F, X020R-X038A-X043R-X101A, X020R-X024R-X116A-X215F, X024R-X043R-X101A-X213A, X014L-X020R-X024R-X043R-X045T-X101A-X215F, X020R-X024R-X215F, X020R-X116A-X215F-X269R, X020R-X045T-X116A-X269R, X020R-X024R-X043R-X045T-X215F,和 X020R-X024R-X043R-X045T-X116A-X213A-X215F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
22.—种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X043R-X076D-X101A-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R-X271F, X024R-X043R-X076D-X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X101A-X103A-X104I -X158E-X188D-X232V-X245R-X248D-X249R-X271F-X271F, X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R-X27IF, XO76D-XIOIG-XIO3A-X1041-X114V-X158E-X188D-X232V-X245R-X248D-X249R-X271F, X024R-X076D-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R-X271F, X024R-X043R-X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X024R-X043R-X101A-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101A-X103A-X104I-X158E-X166D-X188D-X217E-X232V-X245R-X248D-X249R, X076D-X101G-X103A-X104I-X128L-X158E-X188D-X232V-X245R-X248D-X249R-X271F，和 X043R-X076D-X101G-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
23.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X022A-X101G-X103A-X104I-X159D-X217E-X232V-X245R-X248D-X271F, X022A-X043R-X101G-X103A-X104I-X159D-X188D-X217E-X232V-X245R-X248D-X271F, X022A-X101G-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X271F, X043R-X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X043R-X076D-X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R-X271F, X024R-X101G-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R,X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X022A-X076D-X101G-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X271F, X022A-X043R-X076D-X101G-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X271F, X022A-X076D-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X271F, X022A-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X271F, X076D-X101A-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R-X271F, X043R-X101A-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R, X024R-X076D-X101A-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F, X076D-X101A-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101A-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F, X043R-X076D-X101A-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F,X101G-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F, XlOlA-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R-X271F, X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X076D-X101A-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F, X101G-X103A-X104I-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X158E-X188D-X232V-X245R-X248D-X249R, X076D-X101G-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R-X271F, X043R-X076D-X101A-X103A-X104I-X158E-X166D-X188D-X232V-X245R-X248D-X249R,和 X076D-X101A-X103A-X104I-X158E-X188D-X217E-X232V-X245R-X248D-X249R-X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
24.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X017R-X022A-X076D-X101G-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X271F, X022A-X043R-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X271F, X022A-X101G-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X249R-X271F, X017R-X022A-X076D-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X27IF, X022A-X076D-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X249R-X271F, X022A-X101G-X102A-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X271F ,和 X022A-X043R-X076D-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X249R-X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
25.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101S-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101S-X103G-X104V-X158E-X188D-X232V-X245R-X248D-X249R,X101G-X103S-X104I-X158E-X188D-X232V-X245R-X248D-X249R, XlOlA-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101A-X103A-X104L-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103G-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101S-X103G-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101S-X103S-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101S-X103S-X104V-X158E-X188D-X232V-X245R-X248D-X249R, X101A-X103S-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101A-X103S-X104I-X159E-X232V-X245R-X248D-X249R, X101S-X103A-X104I-X159E-X232V-X245R-X248D-X249R, X101G-X103A-X104L-X158E-X188D-X232V-X245R-X248D-X249R, X101A-X103A-X104L-X159E-X232V-X245R-X248D-X249R, X101A-X103S-X104L-X159E-X232V-X245R-X248D-X249R, X101G-X103S-X104L-X159E-X232V-X245R-X248D-X249R, X101S-X103A-X104L-X159E-X232V-X245R-X248D-X249R, X101A-X103G-X104V-X159E-X232V-X245R-X248D-X249R,和 XlOlS-X103A-X104V-X159E-X232V-X245R-X248D-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
26.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X026F-X051W-X104L-X106E, X026F-X031F-X078N-X102A-X160D,X020K-X100S-X116L-X158E-X166D-X243F, X033S-X043W-X218D-X239G-X243F, X022L-X038F-X048R-X062E-X100S-X186K,X101D-X103N-X116L-X144R-X215D, X104L-X105T-X213A-X217E-X256N, X043W-X101D-X212M-X243F, X026F-X048R-X105T-X213A-X218D-X224A, X024F-X101D-X118R-X215D-X250I-X272F, X121F-X185E-X224A-X239G, XO22L-X031F-X102A-X128D-X224A-X243F, X062E-X078N-X102A-X116L-X144R-X250I,X022L-X038F-X121F-X160D-X272F, X026F-X078N-X159C-X186K-X243F, X024F-X048R-X118R-X166D-X217E, X023A-X038F-X078N-X100S-X212M-X215D, XlOOS-Xl16L-X158E-X213A, X078N-X104L-X118R-X128D, X102A-X103N-X105T-X194E，XO22L-X078N-X128D-X213A, X027R-X100S-X118R-X160D-X188D-X243F，X024F-X102A-X186K-X213A-X217E-X243F, X033S-X105T-X188D-X216F，XO23A-X100S-X194E-X212M, X048R-X128D-X185E-X239G, X020K-X024F-X033S-X129E-X194E, X020K-X027R-X129E-X166D-X239G, XO22L-X023A-X027R-X101D-X104L-X216F, X033S-X118R-X129E-X194E-X239G，X022L-X078N-X116L-X129E-X256N, X027R-X101D-X103N-X105T-X272F, X048R-X078N-X116L-X185E-X217E-X239G, X023A-X024F-X027R-X062E, X024F-X103N-X104L-X118R-X188D, X026F-X104L-X25 6N-X272F, X024F-X043W-X104L-X121F-X129E, X062E-X078N-X116L-X224A, X023A-X024F-X051W-X158E, X027R-X038F-X102A-X116L, X062E-X078N-X144R-X212M，X031F-X116L-X256N-X272F, X022L-X033S-X104L-X116L-X160D-X186K, X024F-X118R-X129E-X186K-X213A，X043W-XIO5T-X213A-X215D-X216F, X031F-X105T-X186K-X188D，X026F-X194E-X213A-X256N,和X103N-X160D-X250I-X256N，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
27.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X022A-X024R-X101D-X103A-X104I-X118R-X159D-X188D-X232V-X248D-X271F, X022A-X024R-X103A-X104I-X129E-X159D-X188D-X232V-X248D-X271F, X022A-X024R-X103A-X1041-X118R-X159D-X188D-X217D-X232V-X248D, X022A-X024R-X101D-X103A-X104I-X118R-X129E-X159D-X188D-X232V-X245R-X248D, X022A-X024R-X101D-X103A-X104I-X159D-X188D-X232V-X245R-X248D, X022A-X043R-X103A-X104I-X118R-X129E-X159D-X188D-X232V-X245R-X248D, X022A-X043R-X103A-X104I-X118R-X128I-X129E-X159D-X188D-X232V-X248D, X022A-X043R-X101D-X103A-X104I-X118R-X129E-X159D-X188D-X232V-X248D-X271F, X022A-X024R-X043R-X101D-X103A-X104I-X159D-X188D-X232V-X245R-X248D, X022A-X103A-X104I-X159D-X188D-X232V-X248D,X022A-X024R-X103A-X104I-X118R-X129E-X159D-X188D-X232V-X248D-X271F, X022A-X024R-X103A-X104I-X159D-X188D-X217D-X232V-X245R-X248D-X271F, X022A-X043R-X062E-X103A-X104I-X159D-X188D-X232V-X245R-X248D-X271F, X022A-X043R-X103A-X1041-X129E-X159D-X188D-X232V-X245R-X248D, X022A-X024R-X103A-X104I-X159D-X188D-X217D-X232V-X248D-X271F, X022A-X103A-X104I-X118R-X159D-X188D-X217D-X232V-X245R-X248D, X022A-X024R-X101D-X103A-X104I-X118R-X128I-X159D-X188D-X232V-X245R-X248D, X022A-X024R-X043R-X 103A-X 104I-X 159D-X 188D-X217D-X232V-X248D-X271F，X022A-X043R-X103A-X104I-X118R-X159D-X188D-X217D-X232V-X248D-X271F, X022A-X043R-X103A-X104I-X118R-X159D-X188D-X232V-X248D-X271F, X022A-X103A-X104I-X128I-X129E-X159D-X188D-X232V-X248D-X271F, X022A-X103A-X104I-X159D-X188D-X217D-X232V-X245R-X248D-X271F, X022A-X043R-X103A-X104I-X128I-X159D-X188D-X232V-X245R-X248D, X022A-X101D-X103A-X104I-X118R-X159D-X188D-X217D-X232V-X245R-X248D-X271F, X022A-X103A-X104I-X118R-X129E-X159D-X188D-X232V-X245R-X248D-X271F, X022A-X024R-X043R-X103A-X104I-X118R-X159D-X188D-X217D-X232V-X248D, X022A-X062E-X103A-X104I-X118R-X159D-X188D-X232V-X245R-X248D, X022A-X043R-X101D-X103A-X104I-X118R-X129E-X159D-X188D-X217D-X232V,X022A-X024R-X103A-X104I-X159D-X188D-X217D-X232V-X248D, X022A-X024R-X043R-X103A-X104I-X118R-X128I-X129E-X159D-X188D-X217D-X232V-X248D-X271F，和 X022A-X103A-X104I-X118R-X159D-X188D-X217D-X232V-X245R-X248D-X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
28.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解 活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020K-X024F-X062E-X188D-X239G, X024F-X062E-X116L-X239G, XO20K-X023A-X062E-X188D, X020K-X023A-X024F-X062E-X118R-X188D-X213A, X020K-X043W-X062E-X116L-X188D-X213A-X239G, X023A-X062E-X116L-X118R, X023A-X024F-X062E-X116L-X118R, X024F-X116L, X024F-X062E-X188D-X213A,X023A-X062E-X116L-X118R-X188D-X239G, X020K-X024F-X062E, X020K-X043W-X062E-X116L-X239G, X024F-X062E-X116L-X213A-X239G, X020K-X024F-X043W-X062E-X116L-X213A, X020K-X023A-X024F-X062E-X116L-X188D-X213A, X024F-X062E-X 188D-X239G, X023A-X043W-X062E-X116L-X118R-X213A, X062E-X188D-X239G，X020K-X024F-X062E-X239G, X024F-Xl16L-X118R-X188D-X239G, X020K-X023A-X062E-X116L-X118R-X213A, X020K-X023A-X024F-X062E-X188D-X213A-X239G, X024F-X043W-X118R-X188D, X023A-X024F-X116L-X118R-X188D-X213A, X020K-X023A-X043W-X116L-X188D-X213A-X239G, X023A-X024F-X116L-X188D-X239G, X023A-X043W-X116L-X118R-X188D, X023A-X024F-X118R-X188D-X239G，X023A-X024F-X043W-X062E-X116L-X118R, X020K-X043W-X188D-X213A, X024F-X062E-X118R-X239G, X023A-X043W-X188D-X213A, X020K-X024F-X043W-X062E-X116L-X118R-X188D-X239G, X020K-X116L-X188D-X239G, X020K-X043W-X062E-X118R, XO20K-X043W-X116L-X188D-X213A, X020K-X024F，X023A-X043W-X116L-X239G, X023A-X024F-X043W-X116L-X118R-X188D-X239G, X020K-X023A-X043W-X213A，和 X023A-X024F-X062E-X118R-X213A-X239G，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
29.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020K-X023A-X043W-X118R-X128I-X129E-X159D-X188D, X024F-X118R-X128I-X129E-X159D，X020K-X024F-X062E-X116L-X118R-X188D, X020K-X062E-X116L-X188D, X062E-X116L-X118R-X213A, X020K-X023A-X062E-X116L-X188D, X062E-X116L-X118R-X188D, X020K-X062E-X116L-X213A, X020K-X023A-X062E-X116L, X020K-X062E-X188D-X213A, X020K-X062E, X020K-X024F-X062E-X116L-X188D, X020K-X043W-X062E-X116L-X188D, X020K-X024F-X062E-X188D-X213A, X062E-X116L-X188D-X213A, X020K-X062E-X116L, X020K-X023A-X062E-X116L-X188D-X213A, X023A-X024F-X062E-X116L-X213A, X022A-X043R-X103A-X104I-X128I-X129E-X159D-X188D-X232V-X245R-X248D, X022A-X043R-X103A-X104I-X118R-X128I-X129E-X159D-X188D-X232V-X248D-X271F, X024F-X062E-X116L-X188D, X022A-X024R-X103A-X1041-X118R-X1281-X129E-X159D-X188D-X232V-X248D, X023A-X062E-X116L-X188D, X043W-X062E-X116L, X020K-X023A-X116L-X188D, X043W-X062E-X116L-X188D, X024F-X062E-X116L，X062E-X116L_X188D，和 X022A-X024R-X103A-X104I-X128I-X159D-X188D-X232V-X248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
30.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020K-X023A-X043W-X118R-X128I-X129E-X159D-X188D, X024F-X118R-X128I-X129E-X159D，X020K-X024F-X062E-X116L-X118R-X188D, X020K-X062E-X116L-X188D, X062E-X116L-X118R-X213A, X020K-X023A-X062E-X116L-X188D, X062E-X116L-X118R-X188D, X020K-X062E-X116L-X213A, X020K-X023A-X062E-X116L, X020K-X062E-X188D-X213A, X020K-X062E, X020K-X024F-X062E-X116L-X188D, X020K-X043W-X062E-X116L-X188D, X020K-X024F-X062E-X188D-X213A, X062E-X116L-X188D-X213A, X020K-X062E-X116L, X020K-X023A-X062E-X116L-X188D-X213A, X023A-X024F-X062E-X116L-X213A, X022A-X043R-X103A-X104I-X128I-X129E-X159D-X188D-X232V-X245R-X248D, X022A-X043R-X103A-X104I-X118R-X128I-X129E-X159D-X188D-X232V-X248D-X271F, X024F-X062E-X116L-X188D, X022A-X024R-X103A-X104I-X118R-X128I-X129E-X159D-X188D-X232V-X248D, X023A-X062E-X116L-X188D, X043W-X062E-X116L, X020K-X023A-X116L-X188D, X043W-X062E-X116L-X188D, X024F-X062E-X116L，X062E-X116L_X188D，和 X022A-X024R-X103A-X104I-X128I-X159D-X188D-X232V-X248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
31.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X024R-X086W-X118R, X024R-X078R-X086W-X243F, X024R-X033S-X086S-X087N-X209A, X033S-X118R, X024R-X078R-X086W-X118R-X270T, X024R-X033S-X086W-X118R, X078R-X086W-X243F, X033S-X078R-X086W-X118R-X209A, X033S-X078R-X209A, X086W-X118R-X243F, X024R-X086W, X078R-X086W-X235F, X024R-X118R, X024R-X086R, X101G-X103A-X104I-X232V, X024R-X033S-X078R-X086W-X118R, X024R-X118R-X209A, X209A-X241R, X033S-X086W-X243F, X033S-X172V-X209A, Xl18R-X209A-X243F, X024R-X086S-X141G, X024R-X118R-X209A-X243F, X024R-X033S-X086S-X085N-X235F, X024R-X033S-X133V, X024R-X033S-X078R-X086W, X024R-X086W-X209A, X024R-X241R, X033S-X118R-X243F, X024R-X235F, X024R-X078R-X086W, X024R-X118R-X209A-X235F，X024R-X209A-X241R, X033S-X118R-X241R, X086W-X118R-X209A, X033S-X118R-X159D-X209A, X033S-X078R-X086W, X024R-X086W-X243F, X118R-X209A, X024R-X086W-X118R-X203I, XO78R-X209A-X235F, X024R-X033S-X241R, X078R-X118R, X033S-X118R-X209A-X243F, X021M-X024R-X033S, X024R-X033S-X086W, X033S-X235F, X078R-X086W-X209A, X024R-X033S-X209A-X235F, X033S-X086W-X118R, X024R-X033S-X078R-X209A, X033S-X086W-X118R-X209A-X243F,X086W-X209A-X243F, X005S-X078R-X118R-X241R, X024R-X174T, X033S-X209A-X243F, X086W-X118R-X133V, X024R-X033S-X118R, X024R-X086W-X209A-X235F, X086W-X209A, X008T-X024R, X086W-X118R, X033S-X241R, X005S-X024R-X033S-X243F, X024R-X209A-X242P，X024R-X033S-X078R-X118R, X024R-X033S-X194T, X024R-X243F, X024R-X209A, X024R-X033S-X118R-X209A, X033S-X086W, X024R-X033S, X024R-X033S-X078R-X243F, X086W-X243F, X033S-X118D-X138V-X209A, X033S-X209A-X235F, X024R-X086R-X118R, X033S-X201S, X024R-X239Q, X033S-X118R-X209A-, X078R-X086W, X235F-X243F, X024R-X209A-X235F，X118R-X172V, X017Y-X024R-X033S-X086W, X033S-X148F, X024R-X118R-X235F, X033S-X078R, X033S-X243F, X024C-X033S, X118R-X194T, X033S-X209A, X118R-X209A-X235F，X024R-X033S-X209A-X243F, X024R-X033S-X235F, X024R-X033S-X118R-X235F, X024R-X141G, X024R-X274I, X024R-X033S-X209A, X086W-X235F, X024R-X209A-X243F, X004E-X033S-X078R, X086W-X209A-X235F, X015T-X033S,X033S-X086W-X156L-X209A,X024R-X118R-X243F-X269H,X209A-X235F,X024R-X247H,X024R-X033S-X228T, X078R-X235F, X024R-X033S-X174V-X235F, X024R-X235F-X243F，X024R-X033S-X235F-X241R, X024R-X033S-X151V, X024R-X104A, X033S-X048T, X012H-X104A-X118R,X118R-X235F, X033S-X253A, X143A-X209A, X024R-X033S-X243F, X033S-X239T, X209A-X243F,X024R-X033S-X129H-X184D-X253M, X024R-X085V-X086W-X118R-X235F, X024R-X272P,和X024R-X269C，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
32.—种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X020R-X087D-X101G-X103A-X104I-X232V-X245R, X020R-X101G-X103A-X104I-X150L-X232V-X245R, X018R-X020R-X024R-X076D-X087D-X249R, X018R-X020R-X024R-X076D-X150L-X249R, X018R-X024R-X043R-X076D-X087D-X249R, X018R-X024R-X043R-X076D-X150L-X249R, X018R-X024R-X076D-X078R-X087D-X249R, X018R-X024R-X076D-X078R-X150L-X249R, X018R-X024R-X076D-X087D-X249R-X269R, X018R-X024R-X076D-X087D-X242R-X249R, X018R-X024R-X076D-X087D-X150L-X249R, X018R-X024R-X076D-X150L-X249R, XO18R-X087D-XIOIG-XIO3A-X1041-X232V-X245R, X018R-X101G-X103A-X104I-X150L-X232V-X245R, X018R-X022R-X024R-X076D-X087D-X249R, X018R-X022R-X024R-X076D-X150L-X249R, X043R-X087D-X101G-X103A-X104I-X232V-X245R-X269R, X043R-X101G-X103A-X104I-X150L-X232V-X245R, X024R-X087D-X101G-X103A-X104I-X232V-X245R, X024R-X101G-X103A-X104I-X150L-X232V-X245R, X078R-X087D-X101G-X103A-X104I-X232V-X245R, X078R-X101G-X103A-X104I-X150L-X232V-X245R, X087D-X101G-X103A-X104I-X232V-X245R-X269R, X101G-X103A-X104I-X150L-X232V-X245R-X249R, Xl01G-X103A-X104I-X150L-X232V-X245R-X269R, X022R-X087D-X101G-X103A-X104I-X232V-X245R, X018R-X024R-X043D-X076D-X150L-X249R, X043R-X087D-X101G-X103A-X104I-X232V-X245R, X022R-X101G-X103A-X104I-X150L-X232V-X245R, XO18R-X024R-X043D-X076D-X087D-X249R, X018R-X024R-X076D-X087D-X249R, X018R-X024R-X076D-X150L-X242R-X249R, X043R-X101G-X103A-X104I-X150L-X232V-X245R-X269R, X076D-X101G-X103A-X104I-X150L-X232V-X245R, X087D-X101G-X103A-X104I-X232V-X242R-X245R, X101G-X103A-X104I-X150L-X232V-X245R, X076D-X087D-X101G-X103A-X104I-X232V-X245R, X087D-X101G-X103A-X104I-X232V-X245R，和 X101G-X103A-X104I-X150L-X232V-X242R-X245R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
33.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X024R-X101G-X103A-X104I-X129Q-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X130A-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129Q-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X130A-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129Q-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X130A-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129Q-X130A-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X129Q-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X129Q-X130A-X158E-X188D-X232V-X245R-X248D-X249R,X101G-X103A-X104I-X129Q-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X129Q-X130A-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-。X103A-X104I-X128L-X129Q-X130A-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X128L-X129Q-X158E-X188D-X232V-X245R-X248D-X249R，和 X024R-X027R-X101G-X103A-X104I-X128L-X129Q-X130A-X158E-X188D-X232V-X245R-X248D-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
34.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101G-X103A-X104I-X232V-X222Q-X245R, X101G-X103A-X104I-X158E-X188D-X222S-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X158E-X188D-X222Q-X232V-X245R-X248D-X249R, X076D-X101G-X103A-X104I-X232V-X222Q-X245R, X101G-X103A-X104I-X232V-X222S-X245R, X076D-X101G-X103A-X104I-X232V-X222S-X245R,和 X076D-X101G-X103A-X104I-X158E-X188D-X222S-X232V-X245R-X248D-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
35.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X024R-X101G-X103A-X104I-X128L-X129Q-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X130A-X158E-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X128L-X129Q-X158E-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X129Q-X130A-X158E-X188D-X232V-X245R-X248D-X249R,X101G-X103A-X104I-X130A-X158E-X183D-X188D-X217E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X129Q-X130A-X158E-X188D-X232V-X245R-X248D-X249R, XO24R-X101G-X103A-X104I-X129Q-X158E-X188D-X217E-X232V-X245R-X248D-X249R, XlOlG-X103A-X104I-X128L-X130A-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X129Q-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X129Q-X130A-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X129Q-X158E-X188D-X232V-X245R-X248D-X249R-X271G, XlO1G-X103A-X104I-X129Q-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X130A-X158E-X188D-X217E-X232V-X245R-X248D-X249R, X024R-X101G-X103A-X104I-X128L-X158E-X183D-X188D-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X129Q-X158E-X188D-X217E-X232V-X245R-X248D-X249R，和 X024R-X101G-X103A-X104I-X128L-X130A-X158E-X188D-X232V-X245R-X248D-X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
36.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X1R，X2W，X2M，X2R, X2A, X2S, X3R, X4R, X4C, X4S, X8A, X9F, X9W, X9A, X10S, X10M, XI OH, X10A, X12R, X12F, X14K，X14F, X14Q, X15R, X15F, X16S, X17R, X17M, X17F, X18R, X18K, X20F, X20R, X20K, X22Y, X22A, X22R, X22V, X22Q, X22W, X22L, X23F, X23S, X23A, X24W, X24R, X24H, X24F, X24Q, X24L, X25V, X25F，X25R, X26F, X27V, X27F, X27L, X27R, X28N, X28E, X28A, X29T, X30E, X31F, X33D, X33G, X33S，X34P, X35M, X36F, X36R, X36T, X38L, X38F, X38R, X40L, X40W, X40N, X40R, X40T, X40H, X42I, X43D, X43I, X43R, X43M, X43F, X43W, X43S, X43A, X45T, X46R, X48R, X50C, X51H, X51W, X51F, X52F, X52E, X52N, X55Y, X57R, X59R, X59A, X59F, X60A, X60Q, X60P, X62E, X62Q, X63I, X63V, X63T，X63P, X63D, X63M, X63H, X63Q, X63E, X63A, X63S, X64F, X64T, X68C, X68A, X69N, X69P, X69W,X69T, X71G, X72C, X74C, X75F, X75A, X75R, X75E, X76D, X78I, X78R, X78N, X79W, X79Q, X81R, X82V, X82T, X82F, X82M, X82R, X85M, X86L, X86I, X86W, X89P, X89T, X89V, X89G, X89W, X89H, X89F, X89L, X89I, X91N, X91F, X92F, X94N, X99G, X99F, X99M, X99T, X99P, X100I, X100S, X100N,X100Q, X101N, X101A, X101G, X101P, X101F, X101E, X101T, X101D, X102H, X102N, X102E, X102Τ, X102A, X103G, X103D, X103N, X104D, X104E, X104I, X104L, X105Q, X105E, X105T, X106F,Xl06V, X106G, X106E, X106T, X106D, X106A, X107F, X107M, X108G, X108I, X109M, X111V, X111I,X112V, X112L, X112Q, X114G, X115K, X115R, X116A, X116K,X116L, X117F, X118I, X118R, X119C，X120F, X120A, X120R, X121E, X121F, X123G, X123E, X124S, X128F, X128H, X128I, X128L, Xl28Q, X128N, X128M, X128D, X129E, X132E, X132A, X138G, X144R, X147L, X148I, X158E, X158E,X159E, X159C, X160D, X166D, X166E, X167W, X175V, X177C, X181A, X182R, X183F, X183I,Xl83D, X183R, X183M, X185E, X185I, X185V, X186H, X186K, X188R, X188E, X188D, X192H, X192ff, X194V, X194F, X194E, X197F, X198L, X198F, X203E, X203C, X208S, X209N, X209F, X209E,X209S,X209H, X209G, X209T, X209L, X210R, X210V, X210L, X211R,X211Q, X212I, X212M, X212F，X213A,X214F, X215F, X215N, X215D, X215H, X215E, X216F, X216A, X217N, X217E, X217D, X218P, X218D, X218E, X224A, X224G, X227I, X230E, X231I, X231C, X233C, X234F, X235F, X236F, X236N, X238L, X238K, X238R, X239K, X239S, X239T, X239G, X239H, X239R, X239N, X239F, X240R, X241R, X242L, X242R, X243R, X243F, X244R, X246S, X248I, X248V, X248R, X249R, X249T, X250I,X251S, X251R, X252I, X252F, X252H, X252R, X253F, X253I, X253R, X254C, X256N, X258R, X260V，X260I, X262H, X262D, X263F, X265F, X267N, X267V, X267M, X269I, X269R, X270C, X271F, X271V,X271I, X271P, X271H, X271M, X271T, X271L, X271A, X272F, X272R, X272F, X273I, X273F,和X274G，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
37.根据权利要求35所述的分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X16S, X18R, X20R, X22A, X24R, X43R/D, X45T, X76D, X101A, X103G, X104L,X111V，X128N，X148I，X230E，X242R，和X249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
38.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X1R，X230E，X271L, X115R, X20R, X249R, X235F, X27V/F/L, X75E, X82R, X18R, X269R, X43D, X43R, X76D，X45T, X212F, X242R, X24R, X78R, X9A, X22R, X121E, X244R, X28E, X30E, X4R,和 X241R,其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID N0:1所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
39.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X062E-X158E, X103G-X158E, X128N-X158E，X016S-X158E, X104L-X158E, X089P-X158E, XlllV-X158E, X022A-X158E, X101A-X158E, X148I-X158E, X129E-X158E, X022A-X089P，X016S-X089P, X062E-X089P, X062E-X271F, X158E-X271F, X186H-X271F, X129E-X271F, X111V-X271F, X209E-X271F, X016S-X271F, X188D-X271F, X022A-X271F, X159E-X271F, X104L-X271F, XlOlA-X271F, X089P-X271F, X128N-X271F, X103G-X271F, X148I-X271F, X249R-X271F, X062E-X159E,X016S-X159E, X128N-X159E, X148I-X159E, X111V-X159E, X089P-X159E, X022A-X159E, X129E-X159E, X103G-X159E, X104L-X159E, X158E-X159E, X101A-X159E, X158E-X249R, X111V-X249R, X129E-X249R, X062E-X249R, X016S-X249R, X186H-X249R, X148I-X249R, X159E-X249R, XlO1A-X249R, X188D-X249R, X104L-X249R, X209E-X249R, X022A-X249R, X128N-X249R, X103G-X249R, X089P-X249R, X022A-X111V, X101A-X111V, X016S-X111V, X104L-X111V,X062E-X111V,X103G-X111V, X089P-X111V, X016S-X148I, X062E-X148I, X022A-X148I, X129E-X148I, X104L-X148I,X103G-X148I, X128N-X148I, X101A-X148I, X089P-X148I, X111V-X148I, X016S-X062E, X022A-X062E, X062E-X129E，X022A-X129E，X128N-X129E，X016S-X129E，X101A-X129E，X104L-X129E, X089P-X129E, X103G-X129E, X111V-X129E, X062E-X186H, X128N-X186H, XlOlA-X186H, X022A-X186H, X016S-X186H, X158E-X186H, X089P-X186H, X129E-X186H, X159E-X186H, X103G-X186H, X104L-X186H，X111V-X186H, X148I-X186H, X062E-X101A，X022A-X101A，XO16S-X101A, X089P-X101A, X062E-X103G, X022A-X103G, X016S-X103G, X101A-X103G, X089P-X103G, X062E-X128N, X016S-X128N, X022A-X128N, X101A-X128N, X104L-X128N, X089P-X128N,X103G-X128N, X111V-X128N, X111V-X188D, X062E-X188D, X016S-X188D, X148I-X188D,X022A-X188D, X128N-X188D, X101A-X188D，X104L-X188D,X089P-X188D，X129E-X188D,X159E-X188D, X186H-X188D, X103G-X188D, X158E-X188D, X016S-X022A，X016S-X104L，X022A-X104L，X101A-X104L, X062E-X104L, X103G-X104L， X089P-X104L， X159E-X209E， X111V-X209E,X101A-X209E, X016S-X209E， X128N-X209E， X148I-X209E， X129E-X209E， X062E-X209E，X022A-X209E, X103G-X209E， X158E-X209E， X188D-X209E， X104L-X209E， X089P-X209E，和X186H-X209E，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
40.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X018R-X241R，X020R-X241R, X024R-X241R, X009A-X241R，X020R-X241R, X004R-X241R, X043R-X241R, X078R-X241R, X022R-X241R, X115R-X241R, X001R-X241R, X212F-X241R, X082R-X241R, X018R-X244R ，X024R-X244R, X078R-X244R, X020R-X244R, X212F-X244R, X009A-X244R，X082R-X244R, XOOlR-X244R, X043R-X244R, X022R-X244R, X004R-X244R, X115R-X244R, X241R-X244R, X242R-X244R, X001R-X004R, X009A-X022R，X018R-X022R, X020R-X022R, X004R-X022R, X001R-X022R, XO24R-X242R, X018R-X242R, X004R-X242R, X020R-X242R, X212F-X242R, X082R-X242R, X078R-X242R, X001R-X242R, X009A-X242R，X022R-X242R, X115R-X242R, X043R-X242R, X241R-X242R,X018R-X212F, X022R-X212F, X004R-X212F, X024R-X212F, X001R-X212F, X115R-X212F, X020R-X212F, X009A-X212F, X043R-X212F, X078R-X212F, X082R-X212F, X009A-X078R, X020R-X078R, X024R-X078R, X022R-X078R, X018R-X078R, X004R-X078R, X001R-X078R, X043R-X078R, X022R-X024R, X020R-X024R, X018R-X024R, X001R-X024R, X004R-X024R, X009A-X024R，X004R-X009A, X001R-X009A, X242R-X269R, X024R-X269R, X020R-X269R, X022R-X269R, X249R-X269R, X212F-X269R, X043R-X269R, X244R-X269R, X001R-X269R, X018R-X269R, X078R-X269R, X009A-X269R, X115R-X269R, X241R-X269R, X004R-X269R, X082R-X269R, X018R-X043R,X020R-X043R, X004R-X043R, X022R-X043R, X009A-X043R, X001R-X043R, X024R-X043R, X009A-X018R, XO04R-X018R, X001R-X018R, X024R-X082R, X009A-X082R，X018R-X082R, X001R-X082R, X078R-X082R, X020R-X082R, X022R-X082R, X004R-X082R, X043R-X082R, X043R-X249R, X020R-X249R, X004R-X249R, X018R-X249R, X009A-X249R，X212F-X249R, X022R-X249R, X024R-X249R, Xl15R-X249R, X001R-X249R, X082R-X249R, X242R-X249R, X241R-X249R, X244R-X249R, X078R-X249R, X018R-X115R, X020R-X115R, X022R-X115R, X078R-X115R, X009A-X115R，X004R-X115R, X001R-X115R, X082R-X115R, X043R-X115R, X024R-X115R, X009A-X020R，X018R-X020R, XO04R-X020R, X001R-X020R, X009A-X271L, X020R-X271L, X024R-X271L, X244R-X271L, X241R-X271L, X043R-X271L, X022R-X271L, X249R-X271L, X212F-X271L, X115R-X271L, X242R-X271L, X078R-X271L, X004R-X271L, X269R-X271L, X001R-X271L, X018R-X271L,和 X082R-X271L，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
41.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101G-X103A-X104I,X22A-X101A-X209E, S103G-L111V-G159E, X22A-X103G-X159E，X22A-X111V-X159E, X22A-X128N-X271F-X209E, X22A-X103G-X111V, X62E-X111V-X128N, X22A-X111V-X128N, X22A-X62E-X111V, X101A-X103G-X104L-X188D，X101G-X103A-X104I-X159D，X101A-X103G-X104L-X128N, X22A-X101A-X159E, X101A-X103G-X104L，X101A-X103G-X104L-X159E，X22A-X101A-X103G-X104L, X101A-X103G-X104L-X209E，X22A-X209E-X271F，X22A-X101A-X271F,和X101A-X209E-X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
42.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X103A-X104I-X159D-X232V-X236H-X245R-X248D-X252K, X101G-X104I-X159D-X232V-X236H-X245R-X248D-X252K, X101G-X103A-X159D-X232V-X236H-X245R-X248D-X252K, X101G-X103A-X104L-X232V-X236H-X245R-X248D-X252K, X101G-X103A-X104L-X159D-X236H-X245R-X248D-X252K, XlOlG-X103A-X104L-X159D-X232V-X245R-X248D-X252K, X101G-X103A-X104L-X159D-X232V-X236H-X248D-X252K, X101G-X103A-X104L-X159D-X232V-X236H-X245R-X252K,和 X101G-X103A-X104L-X159D-X232V-X236H-X245R-X248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
43.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101G-X103A-X104I-X159D-X232V-X245R-X248D, X101G-X103A-X104I-X159D-X232V-X245R-X248D-X271F,X101G-X103A-X104I-X159D-X232V-X245R-X248D-X238R, X101G-X103A-X104I-X159D-X232V-X245R-X248D-X248R, X101G-X103A-X104I-X159D-X232V-X245R-X248D-X253R, X101G-X103A-X104I-X159D-X232V-X245R-X248D-X24R, X101G-X103A-X104I-X159D-X232V-X245R-X248D-X76D, X101G-X103A-X104I-X159E-X232V-X245R-X248D-X249R, X101G-X103A-X104I-X159E-X232V-X245R-X248D-X27IF, X101G-X103A-X104I-X158E-X232V-X245R-X248D-X249R, XlO1G-X103A-X104I-X158E-X232V-X245R-X248D-X271F, X22A-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X249R, X22A-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X271F, X62E-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X249R,和 X62E-X101G-X103A-X104I-X159D-X232V-X245R-X248D-X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸 序列的对应关系进行编号。
44.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X20R-X43R-X249R, X20R-X22R-X43R, X20R-X43R-X242R, X20R-X43R-X271L, X20R-X43R-X244R, X20R-X24R-X43R-X242R, X9A-X22R-X78R-X212F-X241R, X9A-X20R-X43R-X212F, X9A-X43R-X212F, X20R-X43R-X212F, X20R-X22R-X43R-X212F, X24R-X78R-X212F, X9A-X43R-X78R, X9A-X43R-X78R-X242R, X9A-X20R-X43R-X78R, X20R-X24R-X43R-X78R-X242R, X22R-X24R-X78R-X212F, X9A-X20R-X43R-X78R-X242R, X20R-X43R-X78R-X249R, X20R-X43R-X78R, X9A-X78R-X212F, X9A-X22R-X43R-X78R, X9A-X20R-X24R-X43R, X9A-X22R-X78R-X212F,X4R-X9A-X22R-X78R-X212F,X20R-X24R-X43R, X1R-X9A-X43R, X20R-X24R-X43R-X115R, X9A-X24R-X43R, X20R-X22R-X24R-X43R, X1R-X24R-X43R, X9A-X20R-X24R-X43R-X242R, X9A-X20R-X22R-X78R-X212F, X9A-X24R-X43R-X244R，X9A-X24R-X43R-X242R，X4R-X9A-X22R-X24R-X212F,以及 X22R-X24R-X43R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
45.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X101G-X103A-X104I-X232V-X245R-X248D, X101G-X103A-X104I-X159D-X232V-X245R, X101G-X103A-X104I-X159R-X232V-X245R-X248D, X101G-X103A-X104I-X159D-X232V-X245R-X248R, XlOlG-X103A-X104I-X232V-X245R, X101G-X103A-X104I-X232V-X245R-X248R, X101G-X103A-X104I-X159R-X232V-X245R-X248R,以 R X101G, X103A, X104I, X232V, X236H, X245R,和 X252K，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
46.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列X16S，X22A, X24R，X62E, X76D, X89P, X101A/G, X103G/A, X104L/I, X111V, X128N, X129E, X232V, X148I, X158E,X159D/E, X166D, X186H, X188D, X209E, X236H, X238R, X245R, X248D/R, X249R, X252K/R, X253R,和X271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
47.一种分离的枯草杆菌蛋白酶变体，其中所述枯草杆菌蛋白酶变体为具有蛋白水解活性的成熟形式并包含具有选自以下的氨基酸置换的组合的氨基酸序列N062E-A158E, S103G-A158E，S128N-A158E，A016S-A158E，V104L-A158E, E089P-A158E，L111V-A158E, T022A-A158E, S101A-A158E, L148I-A158E, P129E-A158E, T022A-E089P, AO16S-E089P, N062E-E089P, N062E-E271F, A158E-E271F, R186H-E271F, P129E-E271F, L111V-E27IF,Y209E-E271F, A016S-E271F, S188D-E271F, T022A-E271F, G159E-E271F, V104L-E271F, SI01A-E271F, E089P-E271F, S128N-E271F, S103G-E271F, L148I-E271F, H249R-E271F, N062E-G159E, A016S-G159E, S128N-G159E，L148I-G159E, LI11V-G159E, E089P-G159E, T022A-G159E，P129E-G159E, S103G-G159E，V104L-G159E，A158E-G159E，S101A-G159E，A158E-H249R, Lll1V-H249R, P129E-H249R, N062E-H249R, A016S-H249R，R186H-H249R, L148I-H249R, G159E-H249R, S101A-H249R, S188D-H249R, V104L-H249R, Y209E-H249R, T022A-H249R, S 128N-H249R,S103G-H249R, E089P-H249R，T022A-L111V, S101A-L111V, A016S-L111V, V104L-L111V, N062E-LIIIV, S103G-L111V, E089P-L111V, A016S-L148I, N062E-L148I, T022A-L148I, P129E-L148I,V104L-L148I, S103G-L148I, S128N-L148I, S101A-L148I，E089P-L148I, L111V-L148I, AOl6S-N062E, T022A-N062E, N062E-P129E，T022A-P129E,S128N-P129E, A016S-P129E, SlOlA-Pl29E,V104L-P129E, E089P-P129E，S103G-P129E，L111V-P129E,N062E-R186H, S128N-R186H, S101A-R186H, T022A-R186H, A016S-R186H, A158E-R186H, E089P-R186H, P129E-R186H, G159E-R186H, S103G-R186H, V104L-R186H, L111V-R186H, L148I-R186H, N062E-S101A, T022A-S101A，A016S-S101A, E089P-S101A, N062E-S103G，T022A-S103G, A016S-S103G，S101A-S103G，E089P-S103G, N062E-S128N, A016S-S128N，T022A-S128N, S101A-S128N, V104L-S128N, E089P-S128N, S103G-S128N, L111V-S128N, L111V-S188D, N062E-S188D，A016S-S188D，L148I-S188D, TO22A-S188D, S128N-S188D, S101A-S188D, V104L-S188D, E089P-S188D, P129E-S188D, G159E-S188D, R186H-S188D, S103G-S188D, A158E-S188D, A016S-T022A, A016S-V104L, T022A-V104L,S101A-V104L, N062E-V104L, S103G-V104L, E089P-V104L, G159E-Y209E， L111V-Y209E,S101A-Y209E, A016S-Y209E，S12 8N-Y209E, L148I-Y209E, P129E-Y209E, N062E-Y209E，T022A-Y209E, S103G-Y209E，A158E-Y209E，S188D-Y209E，V104L-Y209E, E089P-Y209E，和R186H-Y209E，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
48.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列N018R-W241R，G020R-W241R, S024R-W241R, S009A-W241R, G020R-W241R, V004R-W241R, N043R-W241R, S078R-W241R, T022R-W241R, G115R-W241R, A001R-W241R, S212F-W241R, L082R-W241R, N018R-V244R, S024R-V244R, SO78R-V244R, G020R-V244R, S212F-V244R, S009A-V244R, L082R-V244R, A001R-V244R, N043R-V244R, T022R-V244R, V004R-V244R, G 115R-V244R, W241R-V244R, S242R-V244R, AOO1R-V004R, S009A-T022R, N018R-T022R, G020R-T022R, V004R-T022R, A001R-T022R, S024R-S242R, N018R-S242R, V004R-S242R, G020R-S242R, S212F-S242R, L082R-S242R, S078R-S242R, A001R-S242R, S009A-S242R, T022R-S242R, G115R-S242R, N043R-S242R, W241R-S242R, N018R-S212F, T022R-S212F, V004R-S212F, S024R-S212F, A001R-S212F, G115R-S212F, G020R-S212F, S009A-S212F, N043R-S212F, S078R-S212F, L082R-S212F, S009A-S078R, G020R-S078R, S024R-S078R, T022R-S078R, N018R-S078R, V004R-S078R, A001R-S078R, N043R-S078R, T022R-S024R, G020R-S024R, N018R-S024R, A001R-S024R, V004R-S024R, S009A-S024R，V004R-S009A, A001R-S009A, S242R-N269R, S024R-N269R, G020R-N269R, T022R-N269R, H249R-N269R, S212F-N269R, N043R-N269R, V244R-N269R, A001R-N269R, N018R-N269R, S078R-N269R, S009A-N269R, G115R-N269R, W241R-N269R, V004R-N269R, L082R-N269R, N018R-N043R, G020R-N043R, V004R-N043R, T022R-N043R, S009A-N043R, A001R-N043R, S024R-N043R, S009A-N018R, V004R-N018R, A001R-N018R, S024R-L082R, S009A-L082R，N018R-L082R, A001R-L082R, S078R-L082R, G020R-L082R, T022R-L082R, V004R-L082R, N043R-L082R, N043R-H249R, G020R-H249R, V004R-H249R, N018R-H249R, S009A-H249R，S212F-H249R, T022R-H249R, S024R-H249R, G 115R-H249R, A001R-H249R, L082R-H249R, S242R-H249R, W241R-H249R, V244R-H249R, S078R-H249R, N018R-G115R, G020R-G115R, T022R-G115R, S078R-G115R, S009A-G115R, V004R-G115R, A001R-G115R, L082R-G115R, N043R-G115R, S024R-G115R, S009A-G020R，N018R-G020R, V004R-G020R, A001R-G020R, S009A-E271L，G020R-E271L, S024R-E271L, V244R-E271L, W241R-E271L, N043R-E271L, T022R-E271L, H249R-E271L, S212F-E271L, G115R-E271L, S242R-E271L,S078R-E271L, V004R-E271L, N269R-E271L, A001R-E271L, N018R-E271L,和 L082R-E271L，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
49.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-N043R，G020K-N062E，S024F-N116L, G020K-S024F，S024R-A174T, S024R-G118R, S024R-K235F, S024R-P086R, S024R-P086W, S078R-G118R, T033S-G118R, T033S-K235F, Y209A-W241R, G020R-N076D, N018R-Q·245R, S024R-R045T, A232V-Q245R, G118R-A172V, G118R-A194T, I008T-S024R，K235F-N243F，N018R-S103A, N018R-V104I, P086W-G118R, P086W-N243F, P086W-Y209A, S024C-T033S, S02·4R-A232V, S024R-N243F, S024R-P239Q, S024R-S101G, S024R-S141G, S024R-T033S, S024R-T·2741，S024R-Y209A, S078R-P086W, S101G-A232V, T033S-L148F, T033S-P086W, T033S-P201S，T033S-S078R, T033S-W241R, T033S-Y209A, A230E-H249R, A232V-H249R, G118R-K235F, N07·6D-Q245R, P086W-K235F, S024R-R247H, S024R-V104A, S078R-K235F,S101G-H249R,S103A-A232V, T033S-A048T, T033S-P239T, T033S-T253A, T143A-Y209A，Y209A-K235F, N018R-R045T, Y209A-N243F, S024R-A272P, S024R-R269C, S101G-V104I, V104I-A232V, N076D-H249R，和S024R-N076D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
50.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-N076D，S024R-R045T, A230E-H249R, N018R-R045T, N018R-Q245R, S 101G-A232V,S024R-A232V, A232V-Q245R, S024R-S101G,N018R-V104I, N018R-S103A, S101G-H249R，A232V-H249R, S103A-A232V, N076D-Q245R, SlO·1G-V104I, V104I-A232V, N076D-H249R, S024R-N076D, S024F-N116L，G020K-S024F，G020K-N0·62E, T033S-G118R, S024R-P086W, S024R-G118R, S024R-P086R, Y209A-W241R, S024R-W241R, S·024R-K235F, G118R-Y209A, S078R-G118R, T033S-K235F, S024R-A174T, P086W-Y209A, I008T-S024R, P086W-G118R, T033S-W241R, S024R-N243F, S024R-Y209A, T033S-P086W, S024R-T033S，P086W-N243F, T033S-P201S, S024R-P239Q, S078R-P086W, K235F-N243F, G118R-A172V, T033S-L148F, T033S-S078R, T033S-N243F, S024C-T033S, G118R-A194T, T033S-Y209A, S024R-S14·1G,S024R-T274I, P086W-K235F, A015T-T033S, Y209A-K235F, S024R-R247H, S078R-K235F, SO·24R-V104A, T033S-A048T, G118R-K235F, T033S-T253A, T143A-Y209A，T033S-P239T, Y209A-N·243F, S024R-A272P，和S024R-R269C，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
51.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列V004R-S009A-G020R-S242R，G020R-N043R-W241R, G020R-S242R-N269R, V004R-S009A-G020R-N043R, V004R-G020R-H249R, N018R-S024R-V244R, S009A-T022R-S212F-W241R, G020R-N043R-N269R, N018R-S024R-S242R, V004R-S009A-N043R-W241R, G020R-N043R-V244R, G020R-T022R-S242R, V004R-G020R-N043R, VOO·4R-S009A-G020R-N043R-S242R, G020R-N043R-S242R, G020R-N043R-S242R-H249R, G020R-S2·12F-H249R, V004R-S009A-W241R, A001R-S009A-N043R, G020R-N043R-H249R, S009A-G020R-N·043R-W241R, G020R-T022R-N043R, G020R-H249R-N269R, G020R-T022R-W241R, V004R-S009A-S024R-N043R-W241R, S009A-N043R-S078R, V004R-G020R-S024R-V244R, G020R-T022R-S078R-S242R, G020R-S024R-S242R-H249R, V004R-S009A-S078R-W241R, S009A-N043R-S078R-S242R, V004R-G020R-S024R, S009A-N043R-S212F, G020R-N043R-S212F, S024R-S078R-S212F, SOO9A-G020R-S024R-N043R, S009A-T022R-N043R-S078R, G020R-T022R-S212F-W241R, G020R-N043R-S212F-W241R, S009A-N043R-W241R, G020R-N043R-E271L, G020R-T022R-S078R-W241R, G020R-S024R-N043R-S242R, G020R-T022R-N043R-W241R, S009A-G020R-N043R-S212F, V004R-S009A-G020R-S024R-S242R, G020R-N043R-H249R-E271L, G020R-T022R-S024R-S242R, S009A-T022R-S078R-S212F, G020R-N043R-S242R-E271L, S009A-T022R-S078R-S212F-W241R, V004R-G020R-S024R-H249R, G020R-T022R-E271L, G020R-T022R-N043R-S212F, V004R-G020R-S024R-N043R-S242R, V004R-G020R-S024R-N043R, V004R-S009A-T022R-S078R-S212F, G020R-T022R-S078R-S212F-W241R，和G020R-T022R-N269R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
52.—种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列N018R-G020R-N043D-R045T-A230E，N018R-N043R-R045T-S242R-H249R, S024R-N043D-H249R, N018R-G020R-R045T, G020R-S024R-N076D-H249R, S024R-N043R-A230E-S242R, N018R-S024R-N043D-A230E, G020R-N076D, NO18R-S024R-N043D-N076D-H249R, S024R-N043R-N076D-H249R, N018R-S024R-R045T-S242R, G020R-N043D-N076D-A230E-H249R, G020R-N043R-R045T-S242R, N018R-S024R-N076D-H249R, NO18R-G020R-S024R-N043D-R045T-L233I-S242R, S024R-N043R-A230E, N018R-G020R-N043D, N043R-S242R-H249R, G020R-N043R-R045T-A230E, N043R-N076D-S242R-H249R，G020R-S024R-R045T-A230E-S242R, S024R-R045T-N076D-A230E-S242R-H249R, S024R-R045T, S024R-N043R-R045T-N076D-A230E-H249R, N018R-S024R-N043D-R045T-H249R, N018R-N043R-R045T-H249R,S024R-N043R-S242R, N018R-G020R-N043R-N076D-H249R, G020R-S024R-N043D-H249R, G020R-N043R-A230E-S242R, G020R-N043R-S242R, N018R-N043R-N076D-A230E，G020R-S024R-N043D-S242R, G020R-N043R-A230E, N018R-G020R-N043R-N076D-S242R-H249R, N043D-R045T-N076D-H249R, N018R-N043R-S242R-H249R, N018R-G020R-N043R-R045T-S242R, N018R-G020R-N043D-A230E-S242R, G020R-S024R-N043R-R045T-H249R, S024R-N043R-H249R, G020R-S024R-K27E-N043R-N076D-A230E, S024R-N043R-R045T-S242R, N018R-G020R-S024R-N043R-R045T-N076D-A230E, G020R-N043R-N076D-A230E-H249R, N018R-N043R-R045T-S242R, G020R-S242R-H249R, N018R-N043R-N076D-A230E-S242R-H249R, N018R-S024R-N076D, G020R-S024R-K27R-N043D-S242R-H249R, N018R-G020R-S024R-N043D-N076D-S242R, N018R-N043R-N076D-S242R-H249R, N018R-S024R-N043D-A230E-H249R, N018R-G020R-N043D-H249R, N018R-G020R-N043D-R045T-N076D-S242R, S024R-N043R-N076D-A230E-S242R, G020R-S024R-T38I-N043R-R045T-N076D-S242R-H249R, N018R-G020R-N043R, N018R-S024R-R045T-A230E-S242R, N018R-G020R-H249R, S024R-N043R-N076D, N018R-G020R-S024R-N043R-R045T-N076D-H249R, N018R-N043D-R045T-N076D-S242R-H249R, S024R-N043D-S242R-H249R, N018R-G020R-S024R-N043D-R045T-S242R, G020R-S024R-N043R-N076D, N018R-G020R-N043D-R045T-A230E-S242R, GO20R-S024R-N043R-R045T-N076D-S242R-H249R, N018R-N043R-R045T-N076D-S242R, N018R-G020R-N043R-N076D-A230E-S242R, N018R-S024R-N043D-H249R, NO18R-S024R-N043R-R045T-A230E-H249R, N018R-G020R-N043R-R045T-N076D-H249R, N018R-S024R-S242R, N018R-N043R-R045T-N076D-A230E-S242R, R045T-S242R-H249R, N018R-S024R-N043D-S242R，N018R-G020R-N043D-R045T-S240P, S024R-N043R-R045T-S242R-H249R, N018R-S024R-V30S-L31S-D32I-T33Q-G34V-I35F, N018R-G020R-N043R-N076D, G020R-N043D-R045T-N076D-S242R-H249R, NO18R-S024R-N043D-A230E-S242R, N018R-S024R-N043D-S242R-H249R, S024R-N043D-R045T-S242R-H249R, N043R-A230E-H249R, S024R-N043R-N076D-A230E-H249R, G020R-S024R-N043D-N076D-H249R, S024R-R045T-S242R-A273V, G020R-S024R-R045T-N076D-S242R-H249R,N018R-S024R-N043D-N076D-S242R, N018R-N043R-N076D-A230E-H249R, NO18R-G020R-N043R-R045T-H249R, NO18R-N043R-R045T-A230E-S242R, G020R-S024R-N043D-R045T-A230E-S242R, NOl8R-N043D-A230E-H249R, N018R-N043R-N076D-S242R, N018R-G020R-N076D, NO18R-GO2OR-NO43D-N076D-S242R-H249R, G020R-S024R-N043D-N076D-S242R-H249R, N043D-S242R-H249R, N018R-G020R-S024R-N043R-N076D, N018R-G020R-N043D-R045T-N076D-H249R, N018R-G020R-N043R-R045T-N076D-A230E-H249R, N018R-N076D-S242R，G020R-N043R-H249R, N018R-N076D-S242R-H249R, NO18R-S024R-R045T-A230E-H249R, A230E-H249R, N018R-R045T-H249R，G020R-N043R-N076D, N043R-R045T-H249R，N018R-N043D-N076D-S242R-H249R, N043R-N076D-H249R, N018R-R045T, G020R-N076D-A230E-S242R，G020R-S024R-N043D-R045T, S024R-N043D-N076D-S242R-H249R, G020R-R045T-H249R，N043R-N076D-S153A-H249R，N043R-N076D-A230E-H249R, N018R-N043D-N076D-H249R，和 G020R-N043R-N076D-V227I，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
53.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I-A232V-Q236H-Q245R-N252K, S101G-S103A-V104I-A232V-Q245R-N248R, S101G-S103A-V104I-G159R-A232V-Q245R-N248D, S101G-S103A-V104I-G159D-A232V-Q245R-N248R, S101G-S103A-V104I-A232V-Q245R, S101G-S103A-V104I-G159D-A232V-Q245R 和 S101G-S103A-V104I-A232V-Q245R-N248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
54.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I-P129E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248D-H249R, SO24R-S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-G159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128N-P129E-A158E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-L148I-A158E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-G159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128N-P129E-A232V-Q245R-N248D, S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-A158E-G159E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-L148I-A158E-A232V-Q245R-N248D,A016S-S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101 G-S103A-V104I-A158E-G159E-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-P12 9E-A158E-G159E-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A1 58E-G159E-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-S128N-A158E-S 188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D, T022A-S024R-S101G -S103A-V104I-P129E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-S188 D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-A158E-S188D-A232V-N238R-Q245R-N24 8D, T022A-S101G-S103A-V104I-S128N-P129E-S188D-A232V-N238R-Q245R-N248D, S024R-S-101G-S103A-V104I-P129E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-S128N-S188D -A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248 D, T022A-S024R-S101G-S103A-V104I-S128N-P129E-A158E-A232V-Q245R-N248D, S024R-S10 1G-S103A-V104I-G159E-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-S128N-P 129E-A232V-N238R-Q245R-N248D,S024R-S101G-S103A-V104I-P129E-G159E-A232V-Q245R -N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-S188D-A232V-N238R-Q245R-N24 8D, T022A-S024R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D, S024R-S1 01G-S103A-V104I-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-P 129E-A158E-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-L148I-A158E-A232V-Q245R-N248D ，T022A-S101G-S103A-V104I-A158E-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S101 G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-P129E-S18 8D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-A158E-G159E-S188D-A2 32V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-N 238R-Q245R-N248D, S101G-S103A-V104I-P129E-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128N-A158E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A -V104I-S128N-P129E-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-P129E-G159 E-A232V-N238R-Q245R-N248D, S101G-S103A-V104I-P129E-G159E-A232V-Q245R-N248D-H24 9R, T022A-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D-H249R, S024R-S101G-S1 03A-V104I-P129E-L148I-A158E-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-P 129E-G159E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-G159E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-A158E-G159E-A232V-Q245R-N248D -H249R, S024R-S101G-S103A-V104I-P129E-A158E-G159E-A232V-N238R-Q245R-N248D, S024 R-S101G-S103A-V104I-S128N-G159E-S188D-A232V-Q245R-N248D, T022A-S101G-S103A-V10 4I-G159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-G159E-S1 88D-A232V-N238R-Q245R-N248D, S101G-S103A-V104I-A158E-A232V-N238R-Q245R-N248D, T 022A-S101G-S103A-V104I-P129E-G159E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-L148I-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-A158E -A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-A158E-G159E-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-A158E-G159E-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-P129E-A158E-G159E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129E-A158E-S188D-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-G159E-A232V-Q245R-N248D, S101G-S103A-V104I-S188D-A232V-N238R-Q245R-N248D,T022A-S024R-S101G-S103A-V104I-A158E-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-L148I-A158E-A232V-Q245R-N248D, S101G-S103A-V104I-P129E-A158E-G159E-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-G159E-S188D-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-P129E-A232V-N238R-Q245R-N248D, T022A-S024R-S-101G-S103A-V104I-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129E-A158E-G159E-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-A158E-G159E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-G159E-A232V-N238R-Q245R-N248D, S101G-S103A-V104I-S128N-P129E-A232V-Q245R-N248D, S101G-S103A-V104I-A15 8E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129E-G159E-A232V-N238R-Q245R-N248D,S101G-S103A-V104I-A158E-G159E-S188D-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-L148I-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-A158E-A232V-N238R-Q245R-N248D, S101G-S103A-V104I-S128N-P129E-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-S128N-G159E-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-S128N-P129E-A158E-A232V-N238R-Q245R-N248D, S101G-S103A-V104I-S128N-P129E-S188D-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-S128N-P129E-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128N-A158E-G159E-S188D-A232V-Q245R-N248D, T022A-S024K-S101G-S103A-V104I-S128N-A158E-G159E-A232V-Q245R-N248D, S101G-S103A-V104I-P129E-L148I-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-L148I-A232V-Q245R-N248D, T022A-S101G-S103A-V104I-L148I-S188D-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-S128N-P129E-S188D-A232V-Q245R-N248D, S101G-S103A-V104I-S128N-P129E-A158E-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-L148I-A158E-S188D-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-L148I-A158E-S188D-A232V-Q245R-N248D, S101G-S103A-V104I-L148I-G159E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D，和 S101G-S103A-V104I-S128N-P129E-A158E-A232V-Q245R-N248D-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
55. 一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列T022A-S024R-S101G-S103A-V104I-A158E-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-P129E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-G159E-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-A158E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-G159E-S188D-A232V-Q245R-N248D-H249R, T022A-S024R-S101G-S103A-V104I-A158E-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-G159E-S188D-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-L148I-A232V-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-L148I-A158E-A232V-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D-H249R, A016S-S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129E-G159E-A232V-N238R-Q245R-N248D, S024R-S101G-S103A-V104I-P129E-S188D-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-P129E-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-L148I-A158E-A232V-Q245R-N248D, S024R-S101G-S103A-V104 I-P129E-S188D-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-A158E-G159E-S188D-A232V-N238R-Q245R-N248D, T022A-S101G-S103A-V104I-A158E-G159E-A232V-N238R-Q245R-N248D, T022A-S024R-S101G-S103A-V104I-P129E-A158E-A232V-Q245R-N248D, S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, T022A-S101G-S103A-V104I-A158E-G159E-A232V-Q245R-N248D-H249R，和 S024R-S101G-S103A-V104I-A158E-G159E-A232V-Q245R-N248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
56.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列V104L-S128N-A158E-R186H-H249R，S128N-A158E-S188D-H249R, N062E-S128N-A158E-G159E-E271F，N062E-A158E-S188D-H249R-E271F, N062E-A158E-R186H-H249R-E271F, S128N-A158E-S188D-Y209E-E271F, N062E-G159E-S188D-H249R, A016S-N062E-A158E-R186H-H249R, N062E-A158E-G159E-H249R, SlOlA-Sl28N-A158E-Y209E-H249R, S128N-A158E-R186H-E271F, N062E-A158E-S188D-H249R, N062E-A158E-R186H-E271F, N062E-A158E-R186H-H249R, N062E-S101A-R186H-H249R, N062E-S101A-A158E-R186H-E271F, N062E-V104L-A158E-S188D-H249R-E271F, N062E-G159E-R186H-H249R，N062E-G159E-H249R, S 128N-A158E-R186H-H249R, S128N-A158E-S188D-E271F, N062E-A158E-H249R, N062E-R186H-S188D-H249R-E271F，S128N-A158E-Y209E-，N062E-S101A-A158E-H249R, V104L-S128N-A158E-R186H-E271F, N062E-S101A-A158E-R186H-H249R-E271F, A016S-N062E-A158E-H249R, N062E-S10IA-Gl59E-H249R, S128N-A158E-R186H-S188D-E271F，SlOlA-S128N-A158E-R186H-E271F, N062E-S101A-S188D-H249R，S101A-V104L-A158E-R186H-S188D-H249R, N062E-G159E-H249R-E271F, S128N-A158E-G159E-E27IF, A016S-N062E-V104L-A158E-R186H-E271F, T022A-S128N-A158E-H249R, S128N-A158E-H249R，N062E-S101A-V104L-A158E-R186H-E271F, A016S-N062E-A158E-R186H-E271F，V104L-S128N-A158E-H249R, V104L-S-128N-A158E-S188D-H249R, T022A-N062E-A158E, N062E-S101A-S188D-H249R-E271F, N062E-A158E-H249R-E271F, V104L-S128N-A158E-R186H-S188D-E271F, N062E-S101A-R186H-E271F，N062E-V104L-G159E-H249R, N062E-R186H-H249R，N062E-S101A-R186H-H249R-E271F, SlOlA-A158E-R186H-S188D-H249R, N062E-S101A-R186H, SIOIA-S128N-P129E-R186H-H249R, SlO1A-S103G-A158E-R186H-H249R, A016S-N062E-V104L-R186H-S188D-E271F, V104L-A158E-R186H-H249R, S101A-S128N-A158E-S188D-Y209E-E271F, N062E-S101A-R186H-S188D-E271F, A016S-N062E-A158E-H249R-E271F, N062E-S128N-A158E，N062E-S128N-G159E-H249R, N062E-S101A-A158E-S188D-H249R, SlOlA-Sl28N-A158E-H249R, N062E-A158E-R186H-S188D-H249R，A016S-V104L-A158E-R186H-E271F, N062E-L148I-G159E，N062E-S101A-A158E-R186H-H249R, N062E-S101A-R186H-S188D-H249R, V104L-A158E-R186H-S188D-H249R，N062E-S101A-V104L-R186H-S188D-E271F, T022A-S101A-A158E-R186H-H249R, S101A-S128N-A158E-Y209E，Al58E-R186H-S188D-H249R-E271F, V104L-A158E-R186H-S188D-H249R-E271F, S101A-V104L-A158E-R186H-H249R, V104L-A158E-H249R，S101A-V104L-S128N-A158E-R186H-E271F, A016S-V104L-S188D-H249R, S101A-V104L-A158E-R186H-S188D-E271F, V104L-S128N-G159E-E271F，V104L-A158E-R186H-H249R-E271F, A158E-R186H-H249R，S101A-A158E-R186H-H249R，V104L-A158E-S188D-H249R-E271F, A016S-S128N-A158E-R186H，V104L-S128N-R186H-S188D-H249R, A016S-S101A-S128N-R186H, A016S-N062E-S128N-R186H-E271F，A016S-S128N-R186H-E271F, S128N-P129E-R186H, A158E-R186H-H249R-E271F，A016S-A158E-H249R，A016S-A158E-R186H-H249R, A016S-T022A-A158E-R186H-E271F, E089P-S101A-P129E-R186H，T022A-S128N-A158E-R186H, S101A-V104L-S128N-A158E-R186H, T022A-S128N-R186H-S188D-, N062E-V104L-A158E-R186H-S188D-H249R, T022A-A158E-R186H-H249R-E271F, T022A-V104L-A158E-H249R, S101A-L111V-P129E, A016S-A158E-H249R-E271F, A016S-L111V-S188D-, T022A-V104L-R186H-S188D-H249R，和V104L-L148I-S188D-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
57.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列A001R-S101G-S103A-V104I-A232V-Q245R, V004R-S101G-S103A-V104I-A232V-Q245R, N043R-S101G-S103A-V104I-A232V-Q245R-E271L, S078R-S101G-S103A-V104I-A232V-Q245R, V004R-N043R-S101G-S103A-V104I-A232V-Q245R, N018R-N043R-S101G-S103A-V104I-A232V-Q245R, G020R-S101G-S103A-V104I-A232V-Q245R, S101G-S103A-V104I-A232V-Q245R-E271L, G020R-N043R-S101G-S103A-V104I-A232V-Q245R, S024R-N043R-S101G-S103A-V104I-A232V-Q245R, G020R-G025R-N116A-Y167ff, N018R-S101G-S103A-V104I-A232V-Q245R, T022R-S101G-S103A-V104I-A232V-Q245R, SO78R-S103N-S106G-Y167W-Q236N, N018R-N043D-S101G-S103A-V104I-A232V-Q245R-N269R, N043R-S101G-S103A-V104I-A232V-Q245R-N269R, S024R-S101A-H120F-A194F-H249R, G020R-N043D-S101G-S103A-V104I-A232V-Q245R-N269R, S101G-S103A-V104I-S212F-A232V-Q245R，G020R-S144R-N185I-L233C-Q236N, G023A-S078R-S216F-Q236N-H249R，S101G-S103A-V104I-A232V-Q245R-N269R, S101G-S103A-V104I-G115R-A232V-Q245R, P052N-S078R-S 103N-L148I-T213A, N018R-N043D-S101G-S103A-V104I-A232V-Q245R-H249R, S024R-N043D-S101G-S103A-V104I-A232V-Q245R-H249R, S024R-N043D-S101G-S103A-V104I-A232V-Q245R-N269R,G025R-E089I-N116A-P239S-A270C, S024R-S101G-S103A-V104I-A232V-Q245R, L148I-T213A-N252R, S024R-G025R-N183D-Y192W-P239S, G046R-A194F-S212M，V104L-L217E-T224A-H249R-N252R, G023A-Y091F-V121F-Y192W-Q236N, S101G-S103A-V104I-A232V-V244R-Q245R, S099F-S144R-Y167W-N252R, S101G-S103A-V104I-A232V-Q245R-H249R, N043R-S101G-S103A-V104I-A232V-Q245R, T022W-S078R-Y167W-S212M-A270C, V121F-N252R-A270C, G020R-S103N-S216F-Q236N-N252R, N043R-S101G-S103A-V104I-A232V-Q245R-H249R, G023A-P052N-Y192W-I198L-N252R, G025R-G046R-V121F, S024R-S078R-V104L-N116A-N183D, G046R-Q059A-S103N-G211Q-S212M, G020R-P052N-N062Q-Y091F-Y192W, G023A-P052N-S144R-Y192W-S216F, SlOlG-S103A-V104I-A232V -S242R-Q245R, P052N-S103N-N116A-L148I-Y192W, E089I-N116A-N117F-T224A-H249R, S144R-G211Q-N238L-P239S-H249R, N043A-N062Q-A194F-G211Q, G020R-S024R-P052N-Q059A-S216F, S024R-Y167W-T224A-H249R, T057R-Y167W-H249R, G025R-S103N-R186K-A194F-T224A, S105T-S128N-S144R-L148I-S212M，G020R-Q059A-S144R-Y192W-T224A,S024R-N043A-N117F-A194F-G211Q, N117F-A194F-T213A-A270C, S078R-Y091F-V121F-L233C-N252R, T057R-S099F-S105T-I198L-T213A, G023A-Y09IF-S10IA-1198L-N252R, N062Q-S103N-V121F-S144R-H249R, N043R-S101G-S103A-V104I-A232V-S242R-Q245R, G023A-S024R-N117F-S212M-S216F, V104L-T213A-S216F, A194F-G211Q-Q236N, N062Q-S103N-N117F-A194F, SO24R-N062Q-V104L-S106G-H249R, T057R-E089I-I198L, G046R-Q059A-S106G-L217E-H249R, N117F-T213A-A215F, S101A-H120F-Y192W-A215F-T224A, N043A-T057R-N117F-S144R-N183D,G046R-N183D-N238L, G025R-N043A-E089I-N117F，S078R-V104L-T213A-A215F-T224A, Y091F-S099F-S101A-S105T-Y167W, S106G-N117F-N238L，G046R-E089I-Y091F-S101A-N116A, G020R-N062Q-E089I-R186K-S212M, T057R-S099F-V121F-N185I-Y192W, G046R-E089I-Y192W-L233C-A270C, E089I-N117F-N185I-A215F-L233C, P052N-V104L-N183D-S216F-H249R, S078R-S099F-N116A-R186K-T224A, G025R-S105T-S128N-S144R-A270C，S105T-G211Q-S216F, S024R-G046R-Y091F-V121F, S106G-N185I-S216F-Q236N，N062Q-S101A-Q236N-N252R-A270C，G025R-N043A-Y091F-I198L-A270C, G020R-G023A-V104L-Y192W-L233C, S024R-N043A-S105T-S106G-I198L, G020R-E089I-L217E, S024R-Y091F-1198L-A215F-P239S, G046R-E089I-S099F-R186K-S212M, V104L-H120F-R186K-S216F-N252R，T022W-A194F-T213A-L233C-N238L, S099F-S105T-S106G-A194F-S212M, E089I-S105T-N116A-A215F-S216F, G025R-N116A-H120F-T224A-A270C, N043A-Q059A-S101A-S216F-T224A，T057R-N183D-Q236N, G025R-N062Q-S128N-S144R-N185I, S103N-H120F-Y167W-1198L-L233C, T022W-E089I-S216F, S024R-S106G-N116A-S212M-T224A, G020R-P052N-S101A-I198L-L233C, E089I-Y091F-N185I-G211Q-A270C, LlllI-A215F-P239S, S024R-N116A-R186K-L233C-Q236N,和 G023A-S103N-S106G-S212M-A215F,其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
58.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-T022W-S078R-S101A-S103A-V104I-N116S-T213A-A215F-A232V-Q245R, N018R-S078R-S101G-S103A-V104I-A232V-Q245R，S024R-R045T-S 101G-S103A-V104I-A232V-Q245R-N269R, G020R-T022W-S078R-S101G-S103A-V104I-N116A-A232V-Q245R, G020R-T22W-S101G-S103A-V104I-A232V-Q245R, N018R-N043R-S101G-S103A-V104I-A232V-Q245R, NO18R-T022W-S024R-N076D-S10IA-Nl16A-A232V-Q245R, N018R-V104I-A232V-H249R, N018R-S024R-N076D-S 101A-N116A-G211Q-H249R, N018R-N043D-S078R-S101G-S103A-V104I-L217E-A232V-Q245R, N018R-N043R-S101G-S103A-V104I
59.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列N018R-S024R-N043R-N076D-H249R-N269R, N018R-T022R-S024R-N043R-N076D-H249R, N018R-N043D-S101G-S103A-V104I-A232V-Q245R, G020R-N043D-S101G-S103A-V104I-A232V-Q245R, N043D-S101G-S103A-V104I-A232V-Q245R-N269R, N043D-S078R-S101G-S103A-V104I-A232V-Q245R, N043R-N076D-S101G-S103A-V104I-A232V-Q245R, T022R-N043R-S101G-S103A-V104I-A232V-Q245R, N043R-S078R-S101G-S103A-V104I-A232V-Q245R, G020R-N076D-S101G-S103A-V104I-A232V-Q245R, N043R-N076D-S101G-S103A-V104I-A232V-Q245R, T022R-N076D-S101G-S103A-V104I-A232V-Q245R，N076D-S078R-S101G-S103A-V104I-A232V-Q245R, NO18R-S024R-N043R-N076D-H249R, NO18R-S024R-N076D-S242R-H249R, NO18R-S024R-N076D-H249R-N269R，N018R-T022R-S024R-N076D-H249R, N018R-S024R-N076D-S078R-H249R, N018R-S024R-N043D-N076D-H249R-N269R, NO18R-T022R-S024R-N043D-N076D-H249R, N018R-S024R-N043D-N076D-S078R-H249R,G020R-S101G-S103G-V104I-A232V-Q245R, G020R-S101G-S103A-V104L-A232V-Q245R, G020R-S101G-S103A-V104V-A232V-Q245R, G020R-S101G-S103S-V104I-A232V-Q245R, G020R-S101G-S103S-V104L-A232V-Q245R, G020R-S101S-S103S-V104I-A232V-Q245R, G020R-S101S-S103S-V104L-A232V-Q245R, G020R-S101A-S103A-V104L-A232V-Q245R, G020R-S101S-S103S-V104V-A232V-Q245R, G020R-S101S-S103A-V104I-A232V-Q245R, GO20R-SIOIS-S103A-V104V-A232V-Q245R, G020R-S101S-S103G-V104I-A232V-Q245R,G020R-S101S-S103G-V104V-A232V-Q245R, G020R-S101A-S103A-V104V-A232V-Q245R, G020R-S101A-S103S-V104I-A232V-Q245R, G020R-S101A-S103S-V104V-A232V-Q245R, N018R-S024R-N043R-N076D-S078R-H249R, SO24R-N043D-S101G-S103A-V104I-A232V-Q245R, N043D-S101G-S103A-V104I-A232V-Q245R-H249R, S024R-N076D-S101G-S103A-V104I-A232V-Q245R, N076D-S101G-S103A-V104I-A232V-S242R-Q245R, N018R-G020R-S024R-N076D-L217E-H249R, N018R-S024R-N043R-N076D-L217E-H249R, N018R-S024R-N043D-N076D-S242R-H249R, NO18R-G020R-S024R-N043R-N076D-H249R, G020R-S101A-S103G-V104V-A232V-Q245R, N043D-S101G-S103A-V104I-A232V-Q245R, NO18R-S024R-N076D-L217E-H249R-N269R,和 N018R-S024R-N076D-L217E-S242R-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
60.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-S101A-S103A-V104I-G118R-A232V-Q245R, G020R-S024R-N116A-T213A，N043R-S 101A-N116A-A215F-N269R, S024R-N043R-S101A-N116A, S024R-N043R-S101A-N116A-A215F-N269R, G020R-S101G-S103A-V104I-A215F-A232V-Q245R, N043R-S101A-N269R, S024R-N043R-N116A-T213A-N269R, G020R-S024R-N043R-R045T-S101A-T213A, S024R-N043R-N116A-A215F-N269R，G020R-S024R-T213A-A215F,G020R-N116A-N269R, S024R-N116A-T213A-N269R, N043R-S10IA-Nl16A-N269R, S101G-S103A-V104I-N116A-T213A-A232V-Q245R-N269R, S024R-N043R-R045T-S101A-N116A-A215F-N269R, G020R-N043R-S101A-N269R, S101A-S103A-V104I-T213A-A232V-Q245R-N269R, S024R-A215F-N269R, N043R-S101A-N116A-T213A-A215F-N269R, N043R-S101A-T213A-N269R, G020R-S024R-N043R-R045T-N116A-T213A, S101G-S103A-V104I-A232V-Q245R-N269R, S024R-N043R-R045T-S101A-N116A-T213A-N269R, S024R-N043R-R045T-N269R, G020R-N043R-R045T-S101A-N269R, S024R-N043R-N116A-N269R, G020R-S024R-N043R-R045T, N043R-N116A-N269R, S024R-N043R-S101A-A215F-N269R, S024R-N043R-R045T-T213A-A215F-N269R, G020R-S024R-R045T-N269R, G020R-N043R-S101A-N116A-T213A-A215F, G020R-S101G-S103A-V104I-T213A-A215F-A232V-Q245R, G020R-S024R-R045T-N116A-N269R, G020R-S101A-N116A-N269R, S024R-N043R-A215F, G020R-S024R-T213A, S024R-N043R-S101A-A215F，G020R-S024R-N043R-R045T-N116A, G020R-S024R-N043R-R045T-S101A-N269R, G020R-S024R-S101A-A215F, G020R-S024R-N116A-T213A-A215F, G020R-S024R-N116A, G020R-S024R-S101A-N116A, N043R-T213A-A215F-N269R, S024R-S101A-N269R, S024R-N043R-N116A-A215F, G020R-T038A-N043R-S101A, GO20R-S024R-N116A-A215F, S024R-N043R-S101A-T213A，PO14L-G020R-S024R-N043R-R045T-S101A-A215F, G020R-S024R-A215F, G020R-N116A-A215F-N269R, G020R-R045T-N116A-N269R, G020R-S024R-N043R-R045T-A215F,和 G020R-S024R-N043R-R045T-N116A-T213A-A215F,其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
61.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列N043R-N076D-S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R-E271F, S024R-N043R-N076D-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R-E271F-E27IF, S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R-E271F, N076D-S101G-S103A-V104I-A114V-A158E-S188D-A232V-Q245R-N248D-H249R-E271F, S024R-N076D-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R-E271F, S024R-N043R-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-N043R-S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101A-S103A-V104I-A158E-S166D-S188D-L217E-A232V-Q245R-N248D-H249R, N076D-S101G-S103A-V104I-S128L-A158E-S188D-A232V-Q245R-N248D-H249R-E271F,和 NO43R-N076D-S101G-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F,其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
62.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列T022A-S101G-S103A-V104I-G159D-L217E-A232V-Q245R-N248D-E271F, T022A-N043R-S 101G-S 103A-V104I-G159D-S 188D-L217E-A232V-Q245R-N248D-E271F, T022A-S101G-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F, N043R-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R,N043R-N076D-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R-E271F，S024R-S101G-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, T022A-N076D-S101G-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F, T022A-N043R-N076D-S101G-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F,T022A-N076D-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E271F, T022A-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E271F, N076D-S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R-E271F, N043R-S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R, S024R-N076D-S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, N076D-S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, N043R-N076D-S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, S101G-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R-E271F, S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, N076D-S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, S101G-S103A-V104I-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-A158E-S188D-A232V-Q245R-N248D-H249R, N076D-S101G-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R-E271F, N043R-N076D-S101A-S103A-V104I-A158E-S166D-S188D-A232V-Q245R-N248D-H249R,和 N076D-S101A-S103A-V104I-A158E-S188D-L217E-A232V-Q245R-N248D-H249R-E271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
63.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列H017R-T022A-N076D-S101G-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F, T022A-N043R-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E27IF, T022A-S101G-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-H249R-E271F, H017R-T022A-N076D-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E27IF, T022A-N076D-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-H249R-E271F, T022A-S101G-G102A-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F，和 T022A-N043R-N076D-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-H249R-E271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
64.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101S-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101S-S103G-V104V-A158E-S188D-A232V-Q245R-N248D-H249R，S101G-S103S-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101A-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101A-S103A-V104L-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103G-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101S-S103G-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101S-S103S-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101S-S103S-V104V-A158E-S188D-A232V-Q245R-N248D-H249R, SlOlA-S103S-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101A-S103S-V104I-G159E-A232V-Q245R-N248D-H249R, S101S-S103A-V104I-G159E-A232V-Q245R-N248D-H249R, S101G-S103A-V104L-A158E-S188D-A232V-Q245R-N248D-H249R, S101A-S103A-V104L-G159E-A232V-Q245R-N248D-H249R, S101A-S103S-V104L-G159E-A232V-Q245R-N248D-H249R, S101G-S103S-V104L-G159E-A232V-Q245R-N248D-H249R, S101S-S103A-V104L-G159E-A232V-Q245R-N248D-H249R, S101A-S103G-V104V-G159E-A232V-Q245R-N248D-H249R,和 S101S-S103A-V104V-G159E-A232V-Q245R-N248D-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与 如SEQID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
65.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列V026F-V051W-V104L-S 106E, V026 F-L031F-S078N-G102A-S160D, G020K-G100S-N116L-A158E-S166D-N243F, T033S-N043W-N218D-P239G-N243F, T022L-T038F-A048R-N062E-G100S-R186K, S101D-S103N-N116L-S144R-A215D, V104L-S105T-T213A-L217E-S256N, N043W-S101D-S212M-N243F, V026F-A048R-S105T-T213A-N218D-T224A, S024F-S101D-G118R-A215D-L250I-A272F, V121F-N185E-T224A-P239G,T022L-L031F-G102A-S128D-T224A-N243F, N062E-S078N-G102A-N116L-S144R-L250I, T022L-T038F-V121F-S160D-A272F, V026F-S078N-G159C-R186K-N243F, S024F-A048R-G118R-S166D-L217E, G023A-T038F-S078N-G100S-S212M-A215D, G100S-N116L-A158E-T213A, S078N-V104L-G118R-S128D, G102A-S103N-S105T-A194E，T022L-S078N-S 128D-T213A, K027R-G100S-G118R-S160D-S 188D-N243F, S024F-G102A-R186K-T213A-L217E-N243F，T033S-S105T-S188D-S216F, G023A-G100S-A194E-S212M, A048R-S128D-N185E-P239G，G020K-S024F-T033S-P129E-A194E, G020K-K027R-P129E-S166D-P239G, T022L-G023A-K027R-S101D-V104L-S216F,T033S-G118R-P129E-A194E-P239G, T022L-S078N-N116L-P129E-S256N, K027R-S101D-S103N-S105T-A272F, A048R-S078N-N116L-N185E-L217E-P239G, G023A-S024F-K027R-N062E，S024F-S103N-V104L-G118R-S188D, V026F-V104L-S256N-A272F, S024F-N043W-V104L-V12IF-PI29E, N062E-S078N-N116L-T224A, G023A-S024F-V051W-A158E, K027R-T038F-G102A-N116L,N062E-S078N-S144R-S212M, L031F-N116L-S256N-A272F, T022L-T033S-V104L-N116L-S160D-R186K, S024F-G118R-P129E-R186K-T213A, N043W-S105T-T213A-A215D-S216F, L031F-S105T-R186K-S188D, V026F-A194E-T213A-S256N，和 S103N-S160D-L250I-S256N，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID N0:1所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
66.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列T022A-S024R-S101D-S103A-V104I-G118R-G159D-S188D-A232V-N248D-E271F, T022A-S024R-S103A-V104I-P129E-G159D-S188D-A232V-N248D-E271F, T022A-S024R-S103A-V104I-G118R-G159D-S 188D-L217D-A232V-N248D, T022A-S024R-S101D-S103A-V104I-G118R-P129E-G159D-S188D-A232V-Q245R-N248D, TO22A-S024R-S101D-S103A-V104I-G159D-S188D-A232V-Q245R-N248D, T022A-N043R-S103A-V104I-G118R-P129E-G159D-S188D-A232V-Q245R-N248D, T022A-N043R-S103A-V104I-G118R-S128I-P129E-G159D-S188D-A232V-N248D, T022A-N043R-S101D-S103A-V104I-G118R-P129E-G159D-S188D-A232V-N248D-E271F, T022A-S024R-N043R-S101D-S103A-V104I-G159D-S188D-A232V-Q245R-N248D, T022A-S 103A-V104I-G159D-S188D-A232V-N248D, T022A-S024R-S103A-V104I-G118R-P129E-G159D-S188D-A232V-N248D-E271F, T022A-S024R-S103A-V104I-G159D-S188D-L217D-A232V-Q245R-N248D-E27IF, T022A-N043R-N062E-S103A-V104I-G159D-S188D-A232V-Q245R-N248D-E271F, T022A-N043R-S103A-V104I-P129E-G159D-S188D-A232V-Q245R-N248D, T022A-S024R-S103A-V104I-G159D-S188D-L217D-A232V-N248D-E271F, T022A-S103A-V104I-G118R-G159D-S188D-L217D-A232V-Q245R-N248D, T022A-S024R-S101D-S103A-V104I-G118R-S128I-G159D-S188D-A232V-Q245R-N248D, T022A-S024R-N043R-S103A-V104I-G159D-S188D-L217D-A232V-N248D-E271F, T022A-N043R-S103A-V104I-G118R-G159D-S188D-L217D-A232V-N248D-E27IF, T022A-N043R-S103A-V104I-G118R-G159D-S188D-A232V-N248D-E271F, T022A-S103A-V104I-S128I-P129E-G159D-S188D-A232V-N248D-E271F, T022A-S103A-V104I-G159D-S188D-L217D-A232V-Q245R-N248D-E271F, T022A-N043R-S103A-V104I-S128I-G159D-S188D-A232V-Q245R-N248D, T022A-S101D-S103A-V104I-G118R-G159D-S188D-L217D-A232V-Q245R-N248D-E27IF, T022A-S103A-V104I-G118R-P129E-G159D-S188D-A232V-Q245R-N248D-E271F, T022A-S024R-N043R-S103A-V104I-G118R-G159D-S188D-L217D-A232V-N248D, T022A-N062E-S103A-V104I-G118R-G159D-S188D-A232V-Q245R-N248D, T022A-N043R-S101D-S103A-V104I-G118R-P129E-G159D-S188D-L217D-A232V, T022A-S024R-S103A-V104I-G159D-S188D-L217D-A232V-N248D, T022A-S024R-N043R-S103A-V104I-G118R-S128I-P129E-G159D-S188D-L217D-A232V-N248D-E271F,和 T022A-S103A-V104I-G118R-G159D-S188D-L217D-A232V-Q245R-N248D-E271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
67.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020K-S024F-N062E-S188D-P239G，S024F-N062E-N116L-P239G, G020K-G023A-N062E-S188D, G020K-G023A-S024F-N062E-G118R-S 188D-T213A, G020K-N043W-N062E-N116L-S188D-T213A-P239G, G023A-N062E-N116L-G118R, G023A-S024F-N062E-N116L-G118R, S024F-N116L，S024F-N062E-S188D-T213A，G023A-N062E-N116L-G118R-S188D-P239G, G020K-S024F-N062E，G020K-N043W-N062E-N116L-P239G, S024F-N062E-N116L-T213A-P239G, G020K-S024F-N043W-N062E-N116L-T213A, G020K-G023A-S024F-N062E-N116L-S188D-T213A, S024F-N062E-S188D-P239G，G023A-N043W-N062E-N 116L-G118R-T213A, N062E-S188D-P239G，G020K-S024F-N062E-P239G, S024F-N116L-G118R-S188D-P239G, G020K-G023A-N062E-N116L-G118R-T213A, G020K-G023A-S024F-N062E-S188D-T213A-P239G, S024F-N043W-G118R-S188D, G023A-S024F-N116L-G118R-S188D-T213A, GO20K-G023A-N043W-N116L-S188D-T213A-P239G, G023A-S024F-N116L-S188D-P239G, G023A-N043W-N116L-G118R-S188D, G023A-S024F-G118R-S188D-P239G, GO23A-S024F-N043W-N062E-N116L-G118R, G020K-N043W-S188D-T213A, S024F-N062E-G118R-P239G, G023A-N043W-S188D-T213A, G020K-S024F-N043W-N062E-N116L-G118R-S188D-P239G，G020K-N116L-S188D-P239G,G020K-N043W-N062E-G118R, G020K-N043W-N116L-S188D-T213A, G020K-S024F，G023A-N043W-N116L-P239G, G023A-S024F-N043W-N116L-G118R-S188D-P239G, G020K-G023A-N043W-T213A，和G023A-S024F-N062E-G118R-T213A-P239G，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
68.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020K-G023A-N043W-G118R-S128I-P129E-G159D-S188D, S024F-G118R-S128I-P129E-G159D，G020K-S024F-N062E-N116L-G118R-S188D, G020K-N062E-N116L-S188D, N062E-N116L-G118R-T213A, G020K-G023A-N062E-N116L-S188D, N062E-N116L-G118R-S188D, G020K-N062E-N116L-T213A, G020K-G023A-N062E-Nl16L, G020K-N062E-S188D-T213A, G020K-N062E, G020K-S024F-N062E-N116L-S188D, GO20K-N043W-N062E-N116L-S188D, G020K-S024F-N062E-S188D-T213A, N062E-N116L-S188D-T213A, G020K-N062E-N116L, G020K-G023A-N062E-N116L-S188D-T213A, G023A-S024F-N062E-N116L-T213A, T022A-N043R-S103A-V104I-S128I-P129E-G159D-S188D-A232V-Q245R-N248D, T022A-N043R-S103A-V104I-G118R-S128I-P129E-G159D-S188D-A232V-N248D-E271F, S024F-N062E-N116L-S188D, T022A-S024R-S103A-V104I-G118R-S128I-P129E-G159D-S188D-A232V-N248D, G023A-N062E-N116L-S188D, N043W-N062E-N116L, G020K-G023A-N116L-S188D, N043W-N062E-N116L-S 188D, S024F-N062E-N116L, N062E-N116L-S188D，和 T022A-S024R-S103A-V104I-S128I-G159D-S188D-A232V-N248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
69.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S087R-S101G-S103A-V104I-Q109R-S212P-A232V-Q245R-E271V, S101G-S103A-V104I-Q109R-A232V-Q245R, S101G-S103A-V104I-Q109R-S212P-A232V-Q245R-E271V, S101G-S103A-V104I-Q109R-S212P-A232V-Q245R, N076D-S87R-S103A-V104I-S212P-E271V, N076D-S103A-V104I-Q109R, N076D-S103A-V104I-S212P-E271V, N076D-S103A-V104I-Q109R-Q245R,和 N076D-S103A-V104I-S212P-Q245L-E271V，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
70.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S024R-P086W-G118R，S024R-S078R-P086W-N243F, S024R-T033S-P086S-S087N-Y209A, T033S-G118R, S024R-S078R-P086W-G118R-A270T, S024R-T033S-P086W-G118R, S078R-P086W-N243F, T033S-S078R-P086W-G118R-Y209A, T033S-S078R-Y209A, P086W-G118R-N243F, S024R-P086W, S078R-P086W-K235F, S024R-G118R, S024R-P086R, S101G-S103A-V104I-A232V, S024R-T033S-S078R-P086W-G118R, S024R-G118R-Y209A, Y209A-W241R, T033S-P086W-N243F, T033S-A172V-Y209A, G118R-Y209A-N243F,S024R-P086S-S141G, S024R-G118R-Y209A-N243F, S024R-T033S-P086S-S085N-K235F, S024R-T033S-A133V, S024R-T033S-S078R-P086W, S024R-P086W-Y209A, S024R-W241R, T033S-G118R-N243F, S024R-K235F, S024R-S078R-P086W, S024R-G118R-Y209A-K235F, S024R-Y209A-W24IR, T033S-G118R-W241R, P086W-G118R-Y209A, T033S-G118R-G159D-Y209A, T033S-S078R-P086W, S024R-P086W-N243F, G118R-Y209A, S024R-P086W-G118R-V203I, S078R-Y209A-K235F，S024R-T033S-W241R, S078R-G118R, T033S-G118R-Y209A-N243F, L021M-S024R-T033S,S024R-T033S-P086W, T033S-K235F, S078R-P086W-Y209A, S024R-T033S-Y209A-K235F, T033S-P086W-Gl 18R, S024R-T033S-S078R-Y209A, T033S-P086W-G118R-Y209A-N243F, P086W-Y209A-N243F，P005S-S078R-G118R-W241R, S024R-A174T, T033S-Y209A-N243F, P086W-G118R-A133V, S024R-T033S-G118R, S024R-P086W-Y209A-K235F, P086W-Y209A, I008T-S024R，P086W-G118R, TO33S-W241R, P005S-S024R-T033S-N243F, S024R-Y209A-S242P，S024R-T033S-S078R-G118R, S024R-T033S-A194T, S024R-N243F, S024R-Y209A, S024R-T033S-G118R-Y209A, T033S-P086W,S024R-T033S, S024R-T033S-S078R-N243F, P086W-N243F, T033S-G118D-A138V-Y209A, T033S-Y209A-K235F, S024R-P086R-G118R, T033S-P201S, S024R-P239Q, T033S-G118R-Y209A-, SO 78R-P086W, K235F-N243F, S024R-Y209A-K235F, G118R-A172V, H017Y-S024R-T033S-P086W,T033S-L148F, S024R-G118R-K235F, T033S-S078R, T033S-N243F, S024C-T033S, G118R-A194T, T033S-Y209A, Gl18R-Y209A-K235F, S024R-T033S-Y209A-N243F, S024R-T033S-K235F, S024R-T033S-G118R-K235F, S024R-S141G, S024R-T274I, S024R-T033S-Y209A, P086W-K235F, SO24R-Y209A-N243F, V004E-T033S-S078R, P086W-Y209A-K235F, A015T-T033S, T033S-P086W-S156L-Y209A, S024R-G118R-N243F-R269H, Y209A-K235F, S024R-R247H, S024R-T033S-A228T, S078R-K235F, S024R-T033S-A174V-K235F, S024R-K235F-N243F, S024R-T033S-K235F-W241R, S024R-T033S-A151V, S024R-V104A, T033S-A048T, Q012H-V104A-G118R, G118R-K235F,T033S-T253A, T143A-Y209A, S024R-T033S-N243F, T033S-P239T, Y209A-N243F, S024R-T033S-P129H-N184D-T253M, S024R-A085V-P086W-G118R-K235F, S024R-A272P,和 S024R-R269C，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
71.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列G020R-S087D-S IOlG-S 103A-V104I-A232V-Q245R, G020R-S101G-S103A-V104I-V150L-A232V-Q245R, N018R-G020R-S024R-N076D-S087D-H249R, N018R-G020R-S024R-N076D-V150L-H249R, N018R-S024R-N043R-N076D-S087D-H249R, N018R-S024R-N043R-N076D-V150L-H249R, N018R-S024R-N076D-S078R-S087D-H249R, N018R-S024R-N076D-S078R-V150L-H249R, N018R-S024R-N076D-S087D-H249R-N269R,N018R-S024R-N076D-S087D-S242R-H249R, N018R-S024R-N076D-S087D-V150L-H249R, N018R-S024R-N076D-V150L-H249R, N018R-S087D-S101G-S103A-V104I-A232V-Q245R, N018R-S101G-S103A-V104I-V150L-A232V-Q245R, N018R-T022R-S024R-N076D-S087D-H249R, N018R-T022R-S024R-N076D-V150L-H249R, N043R-S087D-S101G-S103A-V104I-A232V-Q245R-N269R, NO43R-S101G-S103A-V104I-V150L-A232V-Q245R, S024R-S087D-S101G-S103A-V104I-A232V-Q245R, S024R-S101G-S103A-V104I-V150L-A232V-Q245R, S078R-S087D-S101G-S103A-V104I-A232V-Q245R, S078R-S101G-S103A-V104I-V150L-A232V-Q245R, S087D-S101G-S103A-V104I-A232V-Q245R-N269R, S101G-S103A-V104I-V150L-A232V-Q245R-H249R, S101G-S103A-V104I-V150L-A232V-Q245R-N269R, T022R-S087D-S101G-S103A-V104I-A232V-Q245R, N018R-S024R-N043D-N076D-V150L-H249R, N043R-S087D-S101G-S103A-V104I-A232V-Q245R, T022R-S101G-S103A-V104I-V150L-A232V-Q245R, N018R-S024R-N043D-N076D-S087D-H249R, N018R-S024R-N076D-S087D-H249R, N018R-S024R-N076D-V150L-S242R-H249R, N043R-S101G-S103A-V104I-V150L-A232V-Q245R-N269R, N076D-S101G-S103A-V104I-V150L-A232V-Q245R, S087D-S101G-S103A-V104I-A232V-S242R-Q245R, S101G-S103A-V104I-V150L-A232V-Q245R, N076D-S087D-S101G-S103A-V104I-A232V-Q245R, S087D-S101G-S103A-V104I-A232V-Q245R,和 S101G-S103A-V104I-V150L-A232V-S242R-Q245R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
72.—种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S024R-S101G-S103A-V104I-P129Q-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S130A-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-S130A-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129Q-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R,和 S024R-K027R-S101G-S103A-V104I-S128L-P129Q-S130A-A158E-S188D-A232V-Q245R-N248D-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
73.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I-A232V-M222Q-Q245R, S101G-S103A-V104I-A158E-S 188D-M222S-A232V-Q245R-N248D-H249R, S 101G-S103A-V104I-A158E-S188D-M222Q-A232V-Q245R-N248D-H249R, N076D-S101G-S103A-V104I-A232V-M222Q-Q245R, S101G-S103A-V104I-A232V-M222S-Q245R, N076D-S101G-S103A-V104I-A232V-M222S-Q245R，和 N076D-S101G-S103A-V104I-A158E-S188D-M222S-A232V-Q245R-N248D-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
74.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S024R-S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R, SlO1G-S103A-V104I-P129Q-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S130A-A158E-N183D-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-S130A-A158E-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-P129Q-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-S128L-S130A-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-S130A-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-P129Q-A158E-S188D-A232V-Q245R-N248D-H249R-E271G, S101G-S103A-V104I-P129Q-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S130A-A158E-S188D-L217E-A232V-Q245R-N248D-H249R, S024R-S101G-S103A-V104I-S128L-A158E-N183D-S188D-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-P129Q-A158E-S188D-L217E-A232V-Q245R-N248D-H249R,和 S024R-S101G-S103A-V104I-S128L -S130A-A158E-S188D-A232V-Q245R-N248D-H249R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ IDNO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
75.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I，T22A-S101A-Y209E, S103G-L111V-G159E, T22A-S103G-G159E，T22A-L111V-G159E, T22A-S128N-E271F-Y209E,T22A-S103G-L111V, N62E-L111V-S128N, T22A-L111V-S128N, T22A-N62E-L111V, SlOlA-S103G-V104L-S188D, S101G-S103A-V104I-G159D, S101A-S103G-V104L-S128N, T22A-S101A-G159E, S101A-S103G-V104L, S101A-S103G-V104L-G159E, T22A-S101A-S103G-V104L, SI01A-S103G-V104L-Y209E, T22A-Y209E-E271F，T22A-S101A-E271F，和 S101A-Y209E-E271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
76.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S103A-V104I-G159D-A232V-Q236H-Q245R-N248D-N252K, S101G-V104I-G159D-A232V-Q236H-Q245R-N248D-N252K, S101G-S103A-G159D-A232V-Q236H-Q245R-N248D-N252K, S101G-S103A-V104L-A232V-Q236H-Q245R-N248D-N252K, S101G-S103A-V104L-G159D-Q236H-Q245R-N248D-N252K, S101G-S103A-V104L-G159D-A232V-Q245R-N248D-N252K, S101G-S103A-V104L-G159D-A232V-Q236H-N248D-N252K, SI01G-S103A-V104L-G159D-A232V-Q236H-Q245R-N252K，和 S101G-S103A-V104L-G159D-A232V-Q236H-Q245R-N248D，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
77.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I-G159D-A232V-Q245R-N248D, S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E271F, S101G-S103A-V104I-G159D-A232V-Q245R-N248D-N238R, S101G-S103A-V104I-G159D-A232V-Q245R-N248D-N248R, S101G-S103A-V104I-G159D-A232V-Q245R-N248D-T253R, S101G-S103A-V104I-G159D-A232V-Q245R-N248D-S24R, S101G-S103A-V104I-G159D-A232V-Q245R-N248D-N76D, S101G-S103A-V104I-G159E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-G159E-A232V-Q245R-N248D-E271F, S101G-S103A-V104I-A158E-A232V-Q245R-N248D-H249R, S101G-S103A-V104I-A158E-A232V-Q245R-N248D-E27IF, T22A-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-H249R, T22A-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E271F, N62E-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-H249R，和 N62E-S101G-S103A-V104I-G159D-A232V-Q245R-N248D-E271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号。
78.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列A1R，A230E, E271L, G115R, G20R, H249R, K235F, K27V/F/L, L75E, L82R, N18R, N269R, N43D, N43R, N76D, R45T, S212F, S242R, S24R, S78R, S9A, T22R, V121E, V244R, V28E, V30E, V4R, W241R, G20R-N43R-H249R, G20R-T22R-N43R, G20R-N43R-S242R, G20R-N43R-E271L, G20R-N43R-V244R, G20R-S24R-N43R-S242R, S9A-T22R-S78R-S212F-W241R, S9A-G20R-N43R-S212F, S9A-N43R-S212F, G20R-N43R-S212F, G20R-T22R-N43R-S212F, S24R-S78R-S212F, S9A-N43R-S78R, S9A-N43R-S78R-S242R, S9A-G20R-N43R-S78R, G20R-S24R-N43R-S78R-S242R, T22R-S24R-S78R-S212F, S9A-G20R-N43R-S78R-S242R, G20R-N43R-S78R-H249R, G20R-N43R-S78R, S9A-S78R-S212F, S9A-T22R-N43R-S78R, S9A-G20R-S24R-N43R, S9A-T22R-S78R-S212F, V4R-S9A-T22R-S78R-S212F, G20R-S24R-N43R, A1R-S9A-N43R, G20R-S24R-N43R-G115R, S9A-S24R-N43R, G20R-T22R-S24R-N43R, A1R-S24R-N43R, S9A-G20R-S24R-N43R-S242R, S9A-G20R-T22R-S78R-S212F, S9A-S24R-N43R-V244R, S9A-S24R-N43R-S242R, V4R-S9A-T22R-S24R-S212F,和 T22R-S24R-N43R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
79.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列S101G-S103A-V104I-A232V-Q245R-N248D, S101G-S103A-V104I-G159D-A232V-Q245R, S101G-S103A-V104I-G159R-A232V-Q245R-N248D, S101G-S103A-V104I-G159D-A232V-Q245R-N248R, S101G-S103A-V104I-A232V-Q245R,S101G-S103A-V104I-A232V-Q245R-N248R, S101G-S103A-V104I-G159R-A232V-Q245R-N248R, S101G, S103A, V104I, A232V, Q236H，和Q245R，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
80.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列A16S，T22A, S24R, N62E, N76D, E89P,S101A/G, S103G/A, V104L/I, L111V, S128N, P129E, A232V, L148I, A158E, G159D/E, R186H, S188D, Y209E, Q236H, Q245R, N248D/R, H249R, N252K/R, T253R，和 E271F，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
81.一种分离的枯草杆菌蛋白酶变体，所述枯草杆菌蛋白酶变体具有蛋白水解活性并包含具有选自以下的氨基酸置换的组合的氨基酸序列A1R，Q2S, Q2M, Q2A, Q2R, Q2W, S3R, V4R, V4S, V4C, I8A, S9A, S9F, S9W, RIOS, R10A, R10H, R10M, Q12F, Q12R, P14K, P14F, P14Q, A15R, A15F, A16S, H17R, H17M, H17F, N18R, N18K, G20F, G20K, G20R, T22A, T22R, T22Y, T22V, T22Q, T22L, T22W, G23A, G23S, G23F, S24R, S24F, S24W, S24Q, S24H, S24L, G25V, G25F, G25R, V26F, K27L,K27F, K27R, K27V, V28A, V28N, V28E, A29T, V30E, L31F, T33S, T33G, T33D, G34P, I35M, S36T, S36F, S36R, T38L, T38F, T38R, P40N, P40L, P40T, P40W, P40H, P40R, L42I, N43A, N43F, N43I, N43S，N43R, N43M, N43W, N43D, R45T, G46R, A48R, F50C, V51W, V51F, V51H, P52F, P52E, P52N, P55Y, T57R, Q59A, Q59F, Q59R, D60P, D60Q, D60A, N62E, N62Q, G63V, G63M, G63T, G63I, G63A, G63S, G63H,G63Q, G63D, G63E, G63P, H64F, H64T, V68A, V68C, A69N, A69T, A69P, A69W, T71G, T71G, I72C,A74C, L75A, L75F, L75E, L75R, N76D, S78R, S78N, S78I, S78R, I79W, I79Q, V81R, L82F, L82T, L82V, L82R, L82M, A85M, P86W, P86L, P86I, E89P, E89T, E89G, E89H, E89W, E89L, E89V, E89W, E89F，E89I, Y91N, Y91F, A92F, K94N, S99F, S99T, S99P, S99G, S99M, G100S, G100N, G100Q, G100I, SI01A, S101N, S101G, S101D, S101T, S101D, S101E, S101P, S101F, G102A, G102T, G102N, G102H, G102E, S103G, S103N, S103D, S103A, V104L, V104I, V104E, V104D, S105T, S105E, S105Q, S106G,S106T,S106E,S106D, S106A, S106V, S106F, I107M, 1107F, A108I, A108G, Q109M, L111V, LlllI，E112V, E112L, E112Q, A114G, G115K, G115R, N116K, N116A, N116L, N117F, G118R, G118I, M119C,H120A, H120F, H120R, V121F, V121F, V121E, N123G, N123E, L124S, S128D, S128F, S128L, S128N, S128H, S128M, S128I, S128Q, P129E, S132A, S132E, A138G, S144R, V147L, L148I, A158E, Gl59D, G159E, G159C, S160D, S166D, S166E, Y167W, M175V, V177C, D181A, Q182R, N183I, N183D, N183M, N183R, N183F, N183R, N185E, N185V, N185I, R186H, R186K, S188E, S188D, S188R, Y192H,Y192W, A194E, A194V, A194F, D197F, D197F, I198L, I198F, V203E, V203C, T208S, Y209S, Y209N，Y209F, Y209T, Y209E, Y209H, Y209G, Y209L, P210R, P210V, P210L, G211Q, G211R, S212I, S212M, S212F, T213A, Y214F, A215N, A215D, A215E, A215H, A215F, S216F, S216A, L217E, L217N, L217D, N218D, N218P, N218E, T224A, T224G, V227I, A230E, A231I, A231C, A232V, L233C, V234F, K235F, Q236F, Q236N, Q236H, N238R, N238K, N238L, P239K, P239G, P239R, P239R, P239H, P239T, P239N, P239S, P239F, S240R, W241R, S242L, S242R, N243F, N243R, V244R, Q245R, I246S, N248D,N248V, N248I, N248R, H249R, H249T, L250I, K251R, K251S, N252I, N252F, N252R, N252K, N252H，T253I, T253R, T253F, A254C, S256N, G258R, T260V, T260I, L262D, L262H, Y263F, S265F, L267V, L267N, L267M, N269I, N269R, A270C, E271I, E271H, E271V, E271H, E271M, E271L, E271P, E271A, E271F, E271T, A272F, A272F, A272R, A273F, A273I，和 T274G，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与如SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氣基酸序列的对应关系进行编号。
82.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQ IDN0:2所示氨基酸序列，并且其中所述蛋白酶变体包含与SEQ ID N0:2的氨基酸序列相差不超过 2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24 或 25 个突变的氨基酸序列，所述突变处于选自氨基酸1、2、3、4、8、9、10、12、14、15、16、17、18、20、22、·23、24、25、26、27、28、29、30、31、33、34、35、36、38、40、42、43、45、46、48、50、51、52、55、57、.59、60、62、63、64、68、69、71、72、74、75、76、78、79、81、82、85、86、89、91、92、94、99、100、101、.102、103、104、105、106、107、108、109、111、112、114、115、116、117、118、119、120、121、123、.124、128、129、132、138、44、147、148、158、159、160、166、167、175、177、181、182、183、185、.186、188、192、194、197、198、203、208、209、210、211、212、213、214、215、216、217、218、224、.227、230、231、233、234、235、236、236、238、238、239、240、241、242、243、244、246、248、249、.250、251、252、253、254、256、258、260、262、263、265、267、269、270、271、272、273 和 274 的氨基酸位置，其中所述枯草杆菌蛋白酶变体的氨基酸位置通过与SEQ ID NO: I所示解淀粉芽孢杆菌(B. amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列的对应关系进行编号，其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所示解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述变体蛋白酶氨基酸序列与所述解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
83.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQ IDNO:2所示氨基酸序列，并且其中所述蛋白酶变体包含与SEQ ID N0:2的氨基酸序列相差不超过 2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24 或 25 个突变的氨基酸序列，所述突变选自 AIR, Q2S, Q2M, Q2A, Q2R, Q2W, S3R, V4R, V4S, V4C, I8A, S9A, S9F，S9W, RIOS, R10A, R10H, R10M, Q12F, Q12R, P14K, P14F, P14Q, A15R, A15F, A16S, H17R, H17M, Hl7F, N18R, N18K, G20F, G20K, G20R, T22A, T22R, T22Y, T22V, T22Q, T22L, T22W, G23A, G23S, G23F，S24R, S24F, S24W, S24Q, S24H, S24L, G25V, G25F, G25R, V26F, K27L, K27F, K27R, K27V, V28A, V28N, V28E, A29T, V30E, L31F, T33S, T33G, T33D, G34P, I35M, S36T, S36F, S36R, T38L, T38F, T38R, P40N, P40L, P40T, P40W, P40H, P40R, L42I, N43A, N43F, N43I, N43S, N43R, N43M, N43W, N43D,R45T, G46R, A48R, F50C, V51W, V51F, V51H, P52F, P52E, P52N, P55Y, T57R, Q59A, Q59F, Q59R, D6OP, D60Q, D60A, N62E, N62Q, G63V, G63M, G63T, G63I, G63A, G63S, G63H, G63Q, G63D, G63E, G63P，H64F, H64T, V68A, V68C, A69N, A69T, A69P, A69W, T71G, T71G, I72C, A74C, L75A, L75F, L75E, L75R, N76D, S78R, S78N, S78I, S78R, I79W, I79Q, V81R, L82F, L82T, L82V, L82R, L82M, A85M, P86ff, P86L, P86I, E89P, E89T, E89G, E89H, E89W, E89L, E89V, E89W, E89F, E89I, Y91N, Y91F, A92F,K94N, S99F, S99T, S99P, S99G, S99M, G100S, G100N, G100Q, G100I, S101A, S101N, S101G, SlOlD，S101T,S101D, S101E, S101P, S101F, G102A, G102T, G102N, G102H, G102E, S103G, S103N, S103D,S103A, V104L, V104I, V104E, V104D, S105T, S105E, S105Q, S106G, S106T, S106E, S106D, SlO6A, S106V, S106F, I107M, I107F, A108I, A108G, Q109M, L111V, L111I, E112V, E112L, E112Q, Al14G, G115K, G115R, N116K, N116A, N116L, N117F, G118R, G118I, M119C, H120A, H120F, H120R, V121F, V121F, V121E, N123G, N123E, L124S, S128D, S128F, S128L, S128N, S128H, S128M, S128I,S128Q, P129E, S132A, S132E, A138G, S144R, V147L, L148I, A158E, G159D, G159E, G159C, S160D，S166D, S166E, Y167W, M175V, V177C, D181A, Q182R, N183I, N183D, N183M, N183R, N183F, N183R, N185E, N185V, N185I, R186H, R186K, S188E, S188D, S188R, Y 192H, Y 192W, A194E, A194V, A194F, D197F, D197F, I198L, I198F, V203E, V203C, T208S, Y209S, Y209N, Y209F, Y209T, Y209E,Y209H, Y209G, Y209L, P210R, P210V, P210L, G211Q, G211R, S212I, S212M, S212F, T213A, Y214F，A215N, A215D, A215E, A215H, A215F, S216F, S216A, L217E, L217N, L217D, N218D, N218P, N218E,T224A, T224G, V227I, A230E, A231I, A231C, A232V, L233C, V234F, K235F, Q236F, Q236N, Q236H, N238R, N238K, N238L, P239K, P239G, P239R, P239R, P239H, P239T, P239N, P239S, P239F, S240R, W241R, S242L, S242R, N243F, N243R, V244R, Q245R, I246S, N248D, N248V, N248I, N248R, H249R, H249T, L250I, K251R, K251S, N252I, N252F, N252R, N252K, N252H, T253I, T253R, T253F,A254C, S256N, G258R, T260V, T260I, L262D, L262H, Y263F, S265F, L267V, L267N, L267M, N269I，N269R, A270C, E271I, E271H, E271V, E271H, E271M, E271L, E271P, E271A, E271F, E271T, A272F，A272F，A272R，A273F，A273I，和T274G，其中所述蛋白酶变体的氨基酸位置根据SEQ IDNO: I所示解淀粉芽孢杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
84.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQ IDN0:2所示氨基酸序列，其中所述蛋白酶变体包含与SEQ ID N0:2的氨基酸序列相差不超过 2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24 或 25 个突变的氨基酸序列，所述突变选自 AIR, Q2S, V4R, V4S, S9A, RIOS, P14K, A16S, H17R, N18R, G20R, T22A, T22R, S24R, S24W, G25R, G25V, V26F, L42I, N43R, N43A, G46R, P52F, P52E, P52N, T57R, Q59A,N62E, N62Q, V68A, V68C, T71G, I72C, A74C. L75A, L75F, L75R, N76D, S78R, L82R, P86W, E89P, E89T, E89G, E89H, E89I, E89V, E89W, Y91N, K94N, G100S, S101A, S101N, S101G, S101D, S103G, SlO3N, V104L, V104I, S106V, S106G, A108I, L111V, E112V, G115K, G115R, N117F, G118I, V121F, SI28D, S128F, S128L, S128N, P129E, S144R, L148I, A158E, G159E, S160D, S166D, N185E, N185I, R186H, S188E, S188D, D197F, V203E, Y209S, Y209N, Y209F, Y209T, Y209E, Y209H, Y209G, P210R,S212I, S212F, Y214F, A215N, A215D, A215E, L217E, L217N, T224A, A230E, A231I, Q236F, N238R，N238K, P239K, P239G, P239R, P239S, W241R, S242R, S242L, N243R, V244R, N248I, N248V, H249R, L250I, N252R, T253R, L262D, Y263F, S265F, L267V, L267N、N269I, N269R, E271F, E271I, E271H, E271P, E271T, E271V, E271L和A272F，并且所述蛋白酶变体任选地包含至少一个选自S103A, G159D, Q236H, Q245R, N248D和N252K的突变，其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所不解淀粉芽抱杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
85.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQID NO:2所示氨基酸序列，其中所述蛋白酶变体包含与SEQ ID NO:2的氨基酸序列相差不超过 2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24 或 25 个突变的氨基酸序列，所述突变选自 G20K, G20R, G23A, S24F, S24R, N43R, N43W, R45T, N62E, N76D，S101A, N116A, N116L, G118R, S128I, P129E, S188D, T213A, A215F, L217E, P239G,和 N269R,其中所述蛋白酶变体的氨基酸位置根据SEQ ID N0:1所示解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
86.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQ IDNO:2所示氨基酸序列，并且其中所述蛋白酶变体包含与SEQ ID N0:2的氨基酸序列相差不超过2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24或25个突变的氨基酸序列，所述突变选自 A16S, T22A, S24R, N62E, N76D, E89P, S101A/G, S103G/A, V104L/I,LI IIV，S128N, P129E, A232V, L148I, A158E, G159D/E, R186H, S188D, Y209E, Q236H, Q245R, N248D/R, H249R, N252K/R, T253R，和E271F，其中所述蛋白酶变体的氨基酸位置根据SEQID NO: I所示解淀粉芽孢杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
87.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQ IDNO:2所示氨基酸序列，并且其中所述蛋白酶变体包含与SEQ ID N0:2的氨基酸序列相差不超过2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24或25个突变的氨基酸序列，所述突变选自 AIR, A230E, E271L, G115R, G20R, H249R, K235F, K27V/F/L, L75E, L82R, N18R, N269R, N43D, N43R, N76D, R45T, S212F, S242R, S24R, S78R, S9A, T22R, V121E, V244R，V28E, V30E, V4R，和W241R，其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所示解淀粉芽孢杆菌Oacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
88.—种迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶的蛋白酶变体，其中所述迟缓芽孢杆菌(Bacillus lentus)枯草杆菌蛋白酶GG36蛋白酶包含SEQID N0:2所示氨基酸序列，并且其中所述蛋白酶变体包含与SEQ ID N0:2的氨基酸序列相差不超过 2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24 或 25个突变的氨基酸序列，所述突变选自AIR, A230E, E271L, G115R, G20R, H249R, K235F, K27V/F/L, L75E, L82R, N18R, N269R, N43D, N43R, N76D, R45T, S212F, S242R, S24R, S78R, S9A, T22R，V121E, V244R, V28E, V30E, V4R，和W241R，并且所述蛋白酶变体任选地包含至少一个选自S103A, G159D, Q236H, Q245R, N248D和N252K的突变，其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所不解淀粉芽抱杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中对应的氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
89.根据权利要求1-26中任一项所述的蛋白酶变体，其中所述蛋白酶变体包含不同于SEQ ID N0:2的氨基酸序列的氨基酸序列，并且其中所述蛋白酶变体的总净电荷相对于迟缓芽孢杆菌(Bacilluslentus)枯草杆菌蛋白酶GG36蛋白酶的总净电荷为O、+1、+2、+3、+4、5、-I、-2、-3、-4或-5，其中亲本蛋白酶为GG36，并且其中所述蛋白酶变体的氨基酸位置根据SEQ ID NO: I所示解淀粉芽孢杆菌(Bacillus amyloliquefaciens)枯草杆菌蛋白酶BPN’的氨基酸序列中的对应氨基酸位置的编号方式来进行编号，这是通过所述蛋白酶变体氨基酸序列与所述解淀粉芽孢杆菌(Bacillusamyloliquefaciens)枯草杆菌蛋白酶BPN’氨基酸序列的比对来确定的。
90.根据权利要求1-89中任一项所述的蛋白酶变体，其中所述蛋白酶变体具有以下特性中的一者或多者a)测试方法2性能指数为至少I. I、至少I. 2、至少I. 3、至少I. 4、至少I. 5、至少I. 6、至少I. 7、至少I. 8、至少I. 9、至少2、1. I至约10、1. I至约8、或甚至I. I至约5 ；b)测试方法3性能指数为至少I. I、至少I. 2、至少I. 3、至少I. 4、至少I. 5、至少I. 6、至少I. 7、至少I. 8、至少I. 9、至少2、I. I至约10、I. I至约8、或甚至I. I至约5 ；c)测试方法4性能指数为至少I. O、至少I. I、至少I. 2、至少I. 3、至少I. 4、至少I. 5、至少I. 6、至少I. 7、至少I. 8、至少I. 9、至少2、I. O至约10、I. O至约8、或甚至I. O至约5 ;和/或d)测试方法6性能指数为至少I. O、至少I. I、至少I. 2、至少I. 3、至少I. 4、至少I. 5、至少I. 6、至少I. 7、至少I. 8、至少I. 9、至少2、I. O至约10、I. O至约8、或甚至I. O至约5。
91.一种分离的核酸，其包含编码权利要求1-90中任一项提供的枯草杆菌蛋白酶变体的多核苷酸序列。
92.一种表达载体，其包含根据权利要求91所述的核酸。
93.一种宿主细胞，其包含根据权利要求92所述的表达载体。
94.一种用于产生芽孢杆菌属(Bacillus)枯草杆菌蛋白酶的至少一种枯草杆菌蛋白酶变体的方法，所述方法包括 a)用包含至少一种编码至少一种根据权利要求1-90中任一项所述的枯草杆菌蛋白酶变体的核酸的表达载体来转化宿主细胞，以产生转化的宿主细胞；并且 b)在适于产生至少一种枯草杆菌蛋白酶变体的条件下培养所述转化的宿主细胞，以产生至少一种枯草杆菌蛋白酶变体。
95.根据权利要求94所述的方法，还包括收获所产生的枯草杆菌蛋白酶变体。
96.根据权利要求92所述的表达载体，其中所述至少一种核酸与启动子有效连接。
97.根据权利要求94-95中任一项所述的方法，其中所述宿主细胞为芽孢杆菌属(Bacillus)的种。
98.根据权利要求97所述的方法，其中所述芽孢杆菌属(Bacillus)的种为枯草芽孢杆菌(B.subtilis)。
99.一种包含至少一种根据权利要求1-90中任一项所述的枯草杆菌蛋白酶变体的组合物，其中所述组合物不是织物和家庭护理产品。
100.根据权利要求99所述的组合物，其中所述组合物为清洁组合物。
101.根据权利要求99-100中任一项所述的组合物，其中所述清洁组合物为颗粒、粉末、固体、条棒、液体、片剂、凝胶或糊剂组合物。
102.根据权利要求99-101中任一项所述的组合物，还包含至少一种漂白剂。
103.根据权利要求99-102中任一项所述的组合物，其中所述清洁组合物不含磷酸盐。
104.根据权利要求99-103中任一项所述的组合物，其中所述清洁组合物含有磷酸盐。
105.根据权利要求99-104中任一项所述的组合物，还包含至少一种另外的酶。
106.根据权利要求105所述的组合物，其中所述至少一种另外的酶选自半纤维素酶、纤维素酶、过氧化物酶、蛋白酶、金属蛋白酶、木聚糖酶、脂肪酶、磷脂酶、酯酶、过水解酶、角质酶、果胶酶、果胶酸裂解酶、甘露聚糖酶、角蛋白酶、还原酶、氧化酶、酚氧化酶、脂加氧酶、木质素酶、支链淀粉酶、鞋酸酶、戍聚糖酶、malanase、β -葡聚糖酶、阿拉伯糖苷酶、透明质酸酶、软骨素酶、漆酶和淀粉酶或它们的任何组合。
107.根据权利要求99-106中任一项所述的组合物，其中所述枯草杆菌蛋白酶变体不是冷水蛋白酶变体。
108.一种清洁方法，包括用包含至少一种根据权利要求1-90中任一项所述的枯草杆菌蛋白酶变体的清洁组合物来接触表面或物品。
109.—种清洁方法，包括用权利要求100-107中任一项示出的清洁组合物来接触表面或物品。
110.根据权利要求108-109中任一项所述的方法，还包括在用所述清洁组合物分别接触所述表面或物品之后漂洗所述表面或物品。
111.根据权利要求108-110中任一项所述的方法，还包括在用所述清洁组合物接触所述表面或物品之后漂洗所述表面或物品的步骤。
112.根据权利要求111所述的方法，还包括在所述表面或物品的所述漂洗之后干燥所述表面或物品的步骤。
113.—种清洁表面或物品的方法，包括提供权利要求100-107中任一项示出的清洁组合物和需要清洁的表面或物品；以及在适于清洁所述表面或物品的所述表面的条件下使所述清洁组合物接触所述需要清洁的表面或物品，以得到洁净的表面或物品。
114.根据权利要求113所述的方法，还包括漂洗所述洁净的表面或物品以得到漂洗过的表面或物品的步骤。
115.根据权利要求113-114中任一项所述的方法，还包括干燥所述漂洗过的表面或物品的步骤。
116.根据权利要求108-115中的任一项所述的方法，其中所述枯草杆菌蛋白酶变体不是冷水蛋白酶变体。
全文摘要
本发明提供由丝氨酸蛋白酶制备的丝氨酸蛋白酶变体。具体地讲，本发明提供与参照丝氨酸蛋白酶相比具有一个或多个置换的丝氨酸蛋白酶变体。此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。在一些实施例中，本发明提供包含这些丝氨酸蛋白酶变体中的至少一种的清洁组合物。
文档编号C11D3/386GK102933708SQ201180022692
公开日2013年2月13日 申请日期2011年5月5日 优先权日2010年5月6日
发明者V·Y·阿列克谢耶夫, N·S·阿明, K·奥古斯汀, L·G·卡斯康-佩雷拉, K·D·科利尔, D·A·埃斯特尔, J·T·小凯利斯, A·J·保罗斯, P·F·苏特, G·S·沃德, J·姚 申请人:丹尼斯科美国公司, 宝洁公司