专利名称：日本血吸虫表皮特异基因、其编码蛋白和应用的制作方法
技术领域：
本发明涉及生物基因领域，具体地涉及一类日本血吸虫表皮特异基因、其编码蛋白，还涉及该日本血吸虫表皮特异蛋白的应用。
背景技术：
血吸虫病由血吸虫的感染引起，在世界上有76个国家，受感染者约1.5亿。致病的血吸虫有3种，既日本血吸虫(Schistosoma japonicum)，曼氏血吸虫(S.mansoni)和埃及血吸虫(S.haematobium)。在我国流行的是日本血吸虫。血吸虫病的流行给人民身体健康和经济发展都造成巨大的损失，因此，防治血吸虫病、开发研制治疗血吸虫病和抗血吸虫的药物具有巨大的社会和经济效益。
研制新的治疗血吸虫病和抗血吸虫的药物，首先必须找到合适的药物作用靶点。血吸虫的表皮是宿主和血吸虫相互作用的界面。而组成血吸虫表皮的蛋白质暴露在这个界面上，对小分子的化学药物和大分子的抗体药物而言都是最理想的药物作用靶点。已经有研究表明治疗血吸虫的化学药物——吡喹酮的作用靶点就是存在于血吸虫的表皮。另外一些已知的血吸虫表皮特异蛋白，例如GST28，paramyosin，已经用于疫苗研究和应用。我们通过大规模的蛋白质组学研究，发现了50个日本血吸虫表皮特异蛋白质，这些蛋白质，都是可能的药物作用靶点和候选疫苗。
鉴于日本血吸虫表皮特异蛋白的重要功能，研究和开发日本血吸虫表皮特异蛋白具有重要意义。
发明内容
本发明要解决的技术问题之一是提供一类日本血吸虫表皮特异基因。
本发明要解决的技术问题之二是提供一类日本血吸虫表皮特异蛋白。
本发明要解决的技术问题之三是提供上述日本血吸虫表皮特异蛋白的应用。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明一个方面，提供了一类日本血吸虫表皮特异基因，其编码具有日本血吸虫表皮特异蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.30所示的序列；
(b)SEQ ID No.1～SEQ ID No.30所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.30所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.30所示的序列。
在本发明的另一方面，提供了上述日本血吸虫表皮特异蛋白，包括(a)具有SEQ ID No.31～SEQ ID No.60所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
较佳地，其具有SEQ ID No.31～SEQ ID No.60所示的氨基酸序列。
在本发明的另一方面，提供了一类能与上述日本血吸虫表皮特异蛋白特异性结合的抗体。在本发明中，可以使用一系列本领域已知的方法来制备针对日本血吸虫表皮特异蛋白的抗体。例如，将提纯的日本血吸虫表皮特异蛋白产物或它的抗原片段注射入动物体内以产生多克隆抗体。同样，表达日本血吸虫表皮特异蛋白或它的抗原片段的细胞也可以用来对动物致免疫而产生抗体。本发明制备的抗体也可以是单克隆抗体，这些单克隆抗体可用杂交瘤技术制备。
在本发明的另一方面，提供了上述日本血吸虫表皮特异蛋白作为免疫原，在制备血吸虫疫苗中的应用。
在本发明的另一方面，提供了上述日本血吸虫表皮特异蛋白在制备防治血吸虫病的药物中的应用。应用本发明的日本血吸虫表皮特异蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物的过程中发挥重要作用。
图1是本发明日本血吸虫表皮特异蛋白的免疫荧光抗体原位杂交实验结果示意图。其中，T代表tegument(表皮)；G代表gut(肠道)；ST代表subtegument(下表皮)，绿色荧光部分是Immunophilin的信号。
具体实施例方式
下面结合附图和实施例对本发明作进一步详细的说明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件，例如Sambrook等人，分子克隆实验室手册(New YorkColdSpring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1日本血吸虫表皮特异蛋白的分离和鉴定实验1.日本血吸虫表皮的分离纯化血吸虫表皮的纯化按照Triton-x-100提取方法进行。表皮特异蛋白从血吸虫雄虫、雌虫、雌雄混合成虫和童虫分离。虫体用Krebs’-Ringer’s，Tris-马来酸盐-缓冲盐液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液悬浮。这个液体和虫体的混和物置于冰水浴中，在摇床上摇动10分钟。然后，装有液体和虫体的混和物的试管在涡旋混合器上混旋几次，然后再放回冰水浴中，静置使虫体下沉。用吸管吸出上部液体，放置到另一个新管中。这个试管在离心机上用2500×g的离心力，离心15分钟。得到的沉淀就是血吸虫的表皮。Krebs’-Ringer’s溶液的配方是0.15MNaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mMTris-Hepes，pH 7.4。Tris maleate缓冲溶液的配方是0.2M Tris and 0.2MMaleicanhyclride，pH 7.4。
2.日本血吸虫表皮特异蛋白的电泳分离日本血吸虫表皮用裂解剂裂解，蛋白溶液用SDS-聚丙烯酰胺凝胶电泳分离，条带切割后再做第二维的在线毛细液相色谱分离以及质谱鉴定。软件分析得到鉴定蛋白。分离好的血吸虫表皮，用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)缓冲液悬浮，用超声波促进组织细胞裂解和蛋白溶解，超声波裂解方法为超声1秒，暂停3秒，总时间2分钟。并用100℃加热5分钟以使蛋白质变性。然后这些表皮特异蛋白混合物用SDS-PAGE分离，分离方法按照普通的SDS凝胶电泳方法进行。胶上电泳泳道用刀切割下来，再用刀均等分割成8-12份。接下来做蛋白的胶内酶解。
3.日本血吸虫表皮特异蛋白的胶内酶解过程胶内酶解按以下方法进行蛋白胶块用刀切碎，每个小块大小约为1立方毫米。加入100mM NH4HCO3，洗2次；转入到试管中；加入10mM二硫苏糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反应1小时；冷却到室温，离心吸去DTT；加入等体积的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室温/黑暗反应45分钟；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，真空抽干；加入100mM NH4HCO3，胶膨胀，去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，抽干；加入含Trypsin的消化缓冲液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以刚覆盖胶为准；37度，过夜酶切；酶切结束后，从试管中吸出上清液到新管子中，胶中加入60％ACN/0.5％HAC，在水浴中超声处理15min，再重复一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，与前面的上清液合并，真空抽气去ACN。
4.日本血吸虫表皮特异蛋白酶解肽段的在线色谱分离和质谱鉴定酶解产生的肽混合物，用真空将溶液体积抽减到30μl。之后，每个组分的肽混合物上样到一个反相捕获柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脱盐。然后这个捕获柱和一个分析用的75μm×150mm C18柱(LC Packings)相连接，肽混合物就在200nl/min的流速下，从这个柱子上洗脱进入装有Protana NanoES的电喷雾离子源的QSTAR pulser i中。Agilent 1100毛细液相系统(Agilent Technologies)用来提供流动相A(0.5％乙酸/水)和流动相B(0.5％乙酸/乙腈)，线性梯度是60分钟内5％B到50％B，30分钟内50％B到90％B，然后是在90％B停留15分钟。在分析柱后通过一个不锈钢的两通(Valco Instrument)连接一个10-μm直径的PicoTip纳喷雾头(New Objective)，加上2500-3000伏的喷雾电压，以获得稳定的喷雾。MS/MS串联质谱谱图通过信息依赖的采集(IDA)和任务-循环增强来记录。在每个测定扫描中，选取3-6个信号最强、带2到3个电荷的离子，用滚动碰撞能量打碎，以获得串联质谱的信息。
5.日本血吸虫表皮特异蛋白的软件搜索鉴定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作为内标，或者以预先数据库搜索得到的得分高的肽段的分子量，来校正所有的质谱谱图。质谱搜索用的数据库，包括从8420个从EST cluster预测得到的血吸虫蛋白质序列，以及可能的污染蛋白质序列如牛胰酶的序列、宿主蛋白污染如人蛋白质序列、小鼠蛋白质序列、大鼠蛋白质序列、兔子蛋白质序列和角蛋白序列等。搜索软件是MASCOT(http://www.matrixscience.com/，Matrix Science)。搜索参数为最大漏切点，3个；monoisotopic；肽电荷数，+1/+2/+3；修饰，半胱氨酸carbamoylmethylation和methionine氧化。搜索结果合并后，用下列标准进行过滤筛选1.对每一个串联质谱谱图的搜索结果列表，只提取排位在第一个的结果，其他的则丢弃；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，则这个肽段丢弃；3.搜索得到的肽段，长度在8个氨基酸以上的保留，小于8个氨基酸的丢弃；4.重复打到的肽段，只计算其中分值最高的，把这些肽段分值加起来，做为蛋白的得分；5.蛋白的得分超过60，就认为是可信的结果。低分的蛋白，手工检查后保留或者丢弃。匹配到宿主或者牛胰酶、角蛋白的肽段，则丢弃。
实施例2日本血吸虫表皮特异蛋白的生物信息学分析和功能注释1.同源性和相似性分析日本血吸虫表皮特异蛋白序列对GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余数据库(Non-redundant，NR)做BLASTP比对；日本血吸虫表皮特异蛋白序列对Swissprot数据库做BLASTP同源性比对。根据比对结果得到的与已知其他物种或已知的血吸虫蛋白的序列相似性，做出日本血吸虫表皮特异蛋白的功能注释。
2.文献查检根据已经发表的文献报道，发现鉴定得到的30个表皮特异蛋白在虫体上的位置，有一些的确在血吸虫表皮上。而其他则是新发现的表皮特异蛋白。举例说明，比如以下表皮分离和质谱鉴定得到的蛋白，根据文献报道，就是已知的表皮特异蛋白，而且有些蛋白已经有进行了疫苗和抗原研究Paramyosin，tropomyosin，myosin，dynein light chain 1，22.6kDa tegument membrane-associated antigen，tegumental antigen Sm20，nitric oxide synthase 1(NOS1)，leucineaminopeptidase，14-3-3 proteins，SnaK，cathepsin B-like cysteine proteaseprecursor，21.7kDa antigen，FK506-binding protein 50(immunophilin)。
实施例3日本血吸虫表皮特异蛋白的免疫荧光抗体原位杂交实验为了进一步用实验的方法验证用蛋白质组学手段鉴定得到的血吸虫表皮特异蛋白，选取了一个Immunophilin(SjFKBP50，GenBank登录号为AY815389)做免疫荧光抗体原位杂交做蛋白定位分析。
实验方法血吸虫成虫用冷冻包埋剂(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗体覆盖切片，在37℃反应60分钟。该抗体是用重组表达的Immunophilin免疫小鼠制备的单克隆抗体。随后切片用PBS洗涤，再加入FITC-偶联的兔抗鼠免疫球蛋白的抗体(1∶40稀释，用含有0.5mg/ml的Evans蓝的PBS稀释)，置湿盒中37℃反应60分钟。染色结果用Leica DM-RB型荧光显微镜观察并拍照。
如图1所示，该实验结果证明了Immunophilin确实分布在血吸虫成虫的表皮上。这说明前面表皮分离和质谱鉴定的结果是可信的。
序列表<110>上海人类基因组研究中心<120>日本血吸虫表皮特异基因、其编码蛋白和应用<130>NP-10276<160>60<210>1<211>1196<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>1gctgaagaaa gggaaatatt agtgctaagc tatttactat ggattataat accgggatag60ctgttcttac tggcggaaat catatatatg tgactaccag tatcgaaact ccacgtatgc120gacgccttgt agaattaccc ggaccagtaa agtctttgtc acagtggatt attgttacga180cacctcttga aacagggtca cctgattctt tccgaagccc atgggtgctt tttgcagaca240aaaagaatct atatcgagct gactttggga cgttagaaaa tattgaactg tctaaactta300tcaaatcaac tggtgttaat attagtttac tgtctatttc tttaaatatg caatttgttg360caatttattt atccaatggt gaattgttaa ttgctaatac acgatttact gaattacatt420cacatatcaa tttaactcaa cgaatatcag tcatgttgat gagtaataac aataataata480atattaataa caataataat actaataata gttcaagtaa tcagaaatta aatcatccaa540agacaatgct ttggttaact aattcagctg taacacttca atggaataat ttagttgcta600ttataagtac acaatcggat atttatgaaa cattttatgc tgacgatata tatttagcac660aagaggttga tggaattcgt atcatttctc ctacatcaca tgaattgtta caacgcgttc720catcaccgct agaagcatta ggacgtatcg gtgcgtcatg tccagccacg tggctattat780ctgcgagtaa aagtttaaaa gcaaattgtg gacgtactaa tgattattta ttgccaatta840aacaaaccaa tcaaatgatt gatgcagtat cacattgtat tgaagcagca tgtcacgcaa900ctgtggatcc tatctgtcaa caagagctac ttgaatcggc tcatttaggt cgaatattat960tatcagctat gtctagtact acacccacta ctactaacgt tactaatgtt aatagtattg1020ataattattc agtggaagtt aaagtgaaag agctcgctga tcatgctgcg aaagtttgcc1080aaactttacg tttactgaat aacttagctg ttccatggtt gggtattgca ttaacatggc1140gtcagtttcg tgaattagga ccaaataaac tgtttgaaag actgttagcc cgctgt1196<210>2<211>723<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>2tctacttaaa gttatgttta cagacggctc tgttctctaa ccgttttaat tttttatgga60atgtcacagt gaaataaatg ttcatacttt aatgtgtcct cattttcgca atggaactag120
agggagtgga ctcatttatt agttcctacg ttgactcact gcaaattctt ccattggaaa180tgcagtctag aattaccgaa taccttcaat cagatctcca acaacgtcat ttattggcac240tggcaaaaca aactatgggc aaactacaaa aatcttcaga gtataatctt aaaaggaagc300atttggagca gcttatatgt gtcttagtag caattcaaaa catatgcgat cgcagaatca360gtggtctaga tgaattagat gaggtgatag aaactcattc tttgaagtta atgaatgcta420aactgaaagt ggatgcaatt agctctcaac ttactcatcc aaatacagaa aatgttcccg480aaagtgaagt cgatatcagt gacgtagaag tatctggtca ccaatcgaat ggtagtttct540tacggcgtaa tttaacgcaa gaattaaatt ttgataagtg tactgctaac tccatgccaa600gaacacgatg taatgatttc agcctactga gtaaagaacg ctttccgctt cgtgctgtta660gcactaataa tcgaactgca acacgaagat cattaaacag gtgggtatcg ggagttcatc720gtc 723<210>3<211>770<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>3cccccccgtc agagttcctc attataaaat gcgtgaaatt gtacaccttc aagttggtca60gtgtggtaac caaattggat caaagttctg ggaagtaatt tcagatgaac atggaatcga120cccatcaggt gaatatcgag gtgattcaga tttgcagcta gaacgaatta atgtttatta180taatgaagca atcggaggaa aatatgttcc acgtgcaata ttagttgatt tagaacctgg240aacaatggac agtgttcgaa caggcccatt tggaaaatta tttcgtccag ataatttcgt300gttcggacaa agtggtgcag gaaacaactg ggctaaaggt cattatactg aaggtgctga360actcgtagat tctgtattag atgtgacacg tagagaggca gagaacacag attctcttca420aggttttcag ttgactcatt cacttggtgg ggggacaggt tcaggtttgg ggactctgtt480gatatcaaag attcgtgagg agtatcctga ccggataata aatacgtttt cagtagttcc540atcaccgaag gtgtccgata ctgttgtcga gccgtacaat gctacacttt ctgtccatca600actcgttgaa aacactgacg aaactttctg tatcgacaat gaagcccttt atgatatatg660cttcagaaca ctgaagttaa ctaatccaac atacggagat ctcaaccact tggtcagtgc720aactatgtct ggggtaacac atgtttgaga tttctggtca actcatgctg 770<210>4<211>1495<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>4ctctctttta aattaatttc agcatgactg aggtggatat taaaatcatc aaacgaacac60aagaaactct agggaaatta ataaaaaaac ctgttcttac cgagaagctt ttgcagaggc120ctccattccg atttctgcat gatatatgta cagcaattat acgttctaca ggactaatga180aaggtctgta tactgcagaa gaactagaag ctgataacgt caaagacaag aataaaaagt240tggcttttct tcagaagtta gtagattttt tggctgttgt acatggtcgt cctattcccg300ttagaataat gagtatcgtt gctggtaaag aggctgagaa aactaacgag atgctgtatt360
tacttgctga cgctgtcaat aagaaggtca acaatcaaga atgtgtagcc catgtacttc420gtggaaatga aaaggataat actaaagcaa ataataccat caaaaagccg ggaccagaga480atgatcaact gaaggggaaa gttaatgtga cacctaattc catggatccc gaaagaaggc540tctatccaca ggaaaataat ttaagttcgg agaaagcaaa tacatctgaa aataggctct600ctatcgatga tgataagagt ttttactctg tgaagaactc atcaggttcg gttaattcgg660aaaaatgtga taccacaaag aaaaatcgtt tatctcaaga ttctggaagt gtcggtggta720accgatgtga ttttcaaaaa ccagaaaagc tgcagaaaga agttgtggtt tctctcccct780caagttcaac aatacctcac caacctcggc atcacagacc agcctccgct aaagggcaga840gggttaagca agatggtaag ctgttttgat gagaattcac agatgtgttt tcattcaaat900ttcttggtaa ctaatccacc tctaatttcc gactcattag aactggttca aatacccgta960taattactac aaatagttat ctctctgaaa ttattctctc ctaaggtaag taagtgatga1020tatattactt gaacaattga ctgttgatac taaatgtcaa tcggttccac ctgtatgttg1080tagtcagtta agcagtttga ttacgattat tggttagtta tggtcaattt agtaactaat1140atcccgtttt atggaaacta tgcaggttca taccaaaagc tttcagaagt gacttttatg1200tcatcttttt tggttttcag taaatatttg ccaaaagtat ttccgctaac caacattttt1260actgaaatat atattcactc ttatttgact gattaattaa tattctttaa aaattgtcgc1320gagtcatggg caaactgtaa tattttctct gttttgaatc aaaaaaagat atacgtcatg1380tcaaaccacc aaagcaaaga taaatattta tatcaattaa tatatcggat atatttggac1440aattaaccta gaatgtgtag gaatactccg agcctgggga gggatatcgc agtcg 1495<210>5<211>664<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>5atcaaattga tgtgataaaa cgtcgcagtg aaaaattgaa tcatgaatta agacgtcaat60tgagtcgatt cattatacga ggacaaaatg gcgatgttga atcagttgca cagttaagaa120aaaattcagt acattcatca tcatcttcat tatgtagtag tcatattaat agtaataata180ataataatac agtatcagta acttccacat ctgatgaaat tatgagtgat ttaaaagtga240atgggaaagt gtcgaatgtt acaatgtcaa aaggttcgtt taatgaaatg cctctaggat300ttttctcaac agctgatttt aaagctatta ttgaccgaat gtcagaagtt caagaagaaa360attgtacact tcgtagatat aaaaaacgtt tagaagcaga tttattaaca aaaagtcaaa420ttatacaaaa tggattggat aattatctta actcttattc attcactgta aaacaatcta480catctaatga tagtaatact tcattatcaa caacaacaac tactactact acatcatcat540catcattccc attgtcaact acatcttatt catcatctat gataacaaat aataatacat600ttcctaatac aacatatggt ttccattcta agatttcact tcaaattcta attcatcatc660atca 664<210>6<211>784<212>DNA<213>日本血吸虫(Schistosoma japonicum)
<400>6cttcatcacc tctacctatt cattctaaaa aaaataatta tgatttaatg aatagaaaac60tagtaaaaca taaggattta ttatcacgtt cgttaaaatt accaacatct aatgaacatg120aatgtaaatt aaaacaatat gaattatgta tagatggtca tcgtgaaata attcaaggaa180ttcacacaac aacatcaaca acattgagca ctacaatgac aatgaataat acaaattcta240gtttaccaac tggttcagaa atgcatgaat caaaatttta taatcaacct gaaaatactg300aaagtacgtg tgtgtttggc cgtgttattg ttgaaataat aaaagaggaa aaatctaatt360tgggtttaac tgtttccgat ggttctaatc aaagtgaagc tccagttatt ttaaatattc420gtcctggaag cattgcagac agaagtgatt gtttcctacc gtatgatcat atattatcaa480tgaatttcac aactgcatca agtgataaca caacttcgag taaacatttc gcatcgaaaa540ttcagatgga aatcggatac gaattaccag cattacctcc tgttggttgt actgttaaac600atatggttgt caatttaaaa attggttcag atggtgttgg tttagtaatt cgaggtggat660ggaataaatc ccctttactt attcgaccgt taactgttat gcatataaga caaaatagta720ctgctgactg tgatggacga ataaaaccgg gcgatcgtat actgacaatt aataatatac780atac 784<210>7<211>1397<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>7ttagtaacgc gttacggcat ggtttcttta aaaaggatag catgcgatga tgtcgcttct60aaatctacgg tttgcaaaca ttcaaagaaa tcaaagtcta ttgattgtct ttccacggaa120gtgtcagaaa atggcattga tttcaataag tcgcatcaaa cttggaattt taagaaagga180gatttcaaag atgaaatgcc taatcataaa tcaaaaaaaa agcttttaaa gaattctatt240tcagaactaa cgaatggaat accaaaatta ttaattacca gtttaccatg tcatatgagt300actaccgcct tgaaagacat ttttccaaca gttgttaatt tgaagatgcg taaaacagct360ggttccaaac atgccgtttt agatttctca tgtaatgagg attatttaga tgccgtgaat420cgtttaaagt caatcgaatt tgatggtgtt aaaccaaatt accgagcagt tgttggacac480acagaaaatg caatccaatc taatctttca aaacaatttg taaataataa tcctaattgt540ttaatactac gaaatctacc atattcactt actgctactg aaattaaaga acattttcca600ctggctgatc gtattcattt agatgtgaat aaatttggaa tatttaacgg ttcttgtata660ttggtaatga aaagtaaaga ggatcttgaa attgttctag ataaatgtaa acataagtat720attaataatc gactggttag agtaaatcta caatccgaat ctaaactgga atcttctaaa780catcatgact caacaaatat gggttatggt ttaaaactta aagaagtacc caatataatt840gaagatgagc atattaaagc attatttcct cagacgtctt tgattggctt aagctcaaga900atatttcaag attccaatac tagaaacgtg ttcatattct tcaaaaagaa ttcttaccgt960aaatctgctt taaaaatgtt caaaactgaa gttgtcttgg gtcatccaat ttcgtgtcga1020ttatgggttt atttttctaa aatatgtatt ccagttccta gtaatcggag atgtaaatct1080cagacagcga aaataaacat ggaagtaaac aacactgttt taaataataa aatgccgaaa1140ccaaatgaat tcagtaagaa tgaaaagaaa ttaaatttgt acgaatgtag gaatgcgtca1200tcatcatcat caattcctaa gaaaatagtt tttgattctg ataacgaata aattatctgg1260cgacagatgc tttccagtgc ctgttttgtg aagtgttatt gtcgcagtat accgaggaga1320
tcgtattagt tccaacaaat tatatcttga ctatatgtaa aaattttgat ttcatattaa1380taaatattac ttagaag 1397<210>8<211>852<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>8aaactaggaa agttgaaatc actgttgact gcagcctgtt gaatgatgct tataccacca60aatcctttga atagtcagac agcatgtgaa ccgtttatat catatggtat ttcacctggg120tttgtcaaac aggtcagaga gaagtttcaa agcgagatat ctatgcagga actgtctaaa180aatttacaac caagagttaa acatcattcg gcaacagctc gtttgacgtg cgcttatgaa240aacttaaagc acaatgatag tggggttcag agacataata catatcccaa cagaagaatt300cagtcttctg gaagagtttc agatacgtta ccttcaacta aattgactaa gtatcttgaa360accaaaaacc acgcagacac tttaaaaatt gataaaaaat gtcctacgct ttcagttggc420ttgagtgaga atgtcaagag taatccagct attcacacta atgattacag accacaaacc480gcagtaaata aataccagct tgacaataat aatggatata gcaatactcc gtcatatgtg540tcaggtactg tacacaatcg aagtcatcct aatgaccaaa atgtttcaaa atacaacgaa600actgataaca aaccaaagga accagatgta tttttagcta ctaagtataa tacgcgaaat660gacttcaagc aaccagcagt actgccaaaa ccacaaaaat gtgagttcac caaagattct720ctgatcaatt taattgtgcc tagaggattc gaagcaagga agcagttaaa tcagcacacg780tataatgaaa aagaacattt ccgtactgtg aaatcagaaa acataccaaa taaaaattta840aaaagcagaa gt852<210>9<211>524<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>9ttaacccagc agatgtttga cgctaagaac atgatggctg catgtgatcc ccgtcatgga60cgttatctaa ctgtcgctgc tatatttcgt ggtcgaatgt ccatgaaaaa agtttatgaa120ccaatgttta acgtccagaa taagaaccca agctactttg ttgattggat tccgaaaaac180gtgaaaacgg ccgttttcga taaacccccc cgaggtttaa aaatgtcagt aacctttatc240ggcaaaagcc cccctatcca aaagttgttc aaggggggat ccgagccgtt tactgctatg300ttttgtcgca aggctttttt acattgggac cccggtgaag gtatgggcga aatggagttc360ccttaaacgg agtcccatat gaatgatttg gtgagtgggg atccccagga tcaggatgcc420actccccaaa aaaagggtga gttttaggag gatatcgaaa atgcataagt ttaataattg480ttaatcgggt tccttttcgc tttcaataaa ttgatgttta atgg 524<210>10<211>739<212>DNA
<213>日本血吸虫(Schistosoma japonicum)<400>10gcgttttgga tggacactca acgtacaaaa tacagttgtc ttactaagcg tttatcccgt60aggctctctc gtttgtctag aaattgtcaa gatgcaactg aagagttttc gattctcctt120caagatctca tcaagcttct tgatcaacat aatgtaaaag tctcgccaga ttcaagcgaa180tctgtgttaa gtttaaattt gatagctgat atgttggatg aaaatgtgtt cagactatta240tctgagttcc accacacatc ttggaaaatt cttacggcac tcacacagcc ttaccaacca300gttgaaaatt cctcaaatgc tagctcactc agtaaatgca attttcccgc tgaaggctct360gttgaaaaat acttgaatca accgcttgat gaagaatact ttatgcgaca cttatttagc420agtactaaac tcttttgcat gttagaaaca aacgtcggag cactggaagc tggtctcatg480tctcaaattt cgtatttgga aggaacttat acggcaaatg aaagtatacc accaaattcc540aacaaagaat gtttaaagca attagaagta gaatgtacca aactagcctc agaattagct600actctaaatc agtcgaaagt tatgcaattg aaatcaagaa atgaattaat tgaaaagcaa660aatcagctaa agcatatgaa tgatattgaa aagattctcc gtcaagaaaa ttcgtgggtt720gcggtttttg acaaactta 739<210>11<211>811<212>DNA<213>日本血吸虫(Schi stosoma japonicum)<400>11gagcaggctg aactggaaga ggaacagaag gagttagata aggaagcagc tgtgctagaa60cggcgtttac gccaacaaat gacatatgaa ccaggcaccg ctctagagga agaactatat120aaaagatggt ttattttagt cagtaaaaag aatgctttaa ttcaccgtgg cttccagcta180tctataatgg agaaggaaga tgatttgaaa aagaaaatga atatgttgca agacgagtta240cgatcgatct tatccattga agaatattta aaaacagata atgacagaaa acgagaagat300cttcttctac aagatctagt acggttggtc aatgaacgtg atgatatgat tcaggaattg360gacttacatg aaaaagcatt atctgaagaa atggatttgg gacagaatac aagttttgtg420ccgcatggga gacttaaagt tgataagagt tgtgtactgc agtgatatcc aatttagtca480tactactatg tttcactgtt tacttctgct ctaaacttat tattcattgt tttttcaaca540acaaaaaaaa cagtattaat gaactccgtt tcagcgccat acttcgtttg ctgtctcagg600cgattacttt tttgtgtttt ccccagattt catttactta gtcttatttc tgaattttca660attacatcaa aagtgttgtc gctcctcggt ttccctatca tcgtctaatg cttcagaaaa720ctttgtttac tttttgatat ttgtttaaca tagtaagtta ttttgcaaac agagtcacta780caaacgtgac tgtaaactat gatctgtcat c 811<210>12<211>1088<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>12
aggattacaa cagtgacgat gatgatgaca agagactatg ctaggatcat tttcccaaac60aaagcagata ccaatctcct cagatcatgt gcgtgttata gcctcttcat gtattggctt120tattgttgtt gcttctgaag ccactattga cggtttccca caaagcgacg acgagaaaag180gacgaagaaa ctggaacttg tgaattcact tgagcgtaaa ctatgcctgt cgtcatctgc240taaacgagac gagaaatgga catttactca aagtcagggt attgctcttc tcatcccatt300aaagcacata ccaacagttt tgatcaattc agaaacaata cagtcaggtt tttgtgaact360cttggggcgt tttattaaac gattatgcat tcatgaaaac cctgctatct gtcaaatcgg420gtaccgatgt gttggatgtt ttattagtca tttaacagca aaccatgatg taacttacga480acctaaagct ttattagaac tacttggcaa aggtttcgaa cattcagtaa ttgatatgcg540tatgttatca actgtagttt ccaatcatat tgcatggcat gtgaaacttc cgatgccttc600ttggatttct acatttgtga atatacttct ggctgggaca aaagataaaa atagtccagt660tcgacttgga agtgaaacag ctctggctgt tctttgtcgt ataagtgctc ctaagagtaa720taaagataag tgcccaaatt caggatattt acaagcttgt tacgacgcat tggataataa780tacacgtaat caattagaaa cacttgtaca acgccttcgt aaacaaaatt ggtctgaagt840ttggcgtcaa ggttgccctg atatggataa tacaaattca ttgtaaatct atagaatgct900gtataaacat tcaagccttt cctttttctt tattactaat tgatcgaaat tgtatttaca960tctcattcaa acagtccact ctgtatcacg attttcccag tttgtttgga atatatacat1020atatttgaca aagattgtta ttttctagct ttcattgatt cagtcttcat taaatacatt1080tgctctac 1088<210>13<211>1391<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>13aactatcttc ctgttttctg tctattctgg aatttttcgt tatttaggtc cattgtactt60gcattgctaa tatcaaacga tcgaagccgt cttcagatat caatacaccc attgatcaaa120agaagaaacg cttgcataat aatagttcat cggtttctat ggagaaaaaa aattgtgttg180atacatttac tggctggtca agtggtgatc aggttgactt tatttgcaat ttattgtcga240aaatttctca ctttcaacaa agccaaatta atgacttttt agaacctctc cttaagcgcg300atttcatcgt tgcacttcaa aatcgcggtg tcccgtatct cgctgaaatg attttagtcc360aattagatgc atccagtttg ctttcagttg aacttgcgtc taggggatgg cagtggagtg420ttgcaaaata ccagctttgg cgtcgtctac tggctcgccg tgtagctaca gatccagttt480ggcatggtct atgcgaacgt agaggatgga ttgaatttat cgagcattct gacccagctt540atttacctgc actactcgaa aggcgtcgag ttcctttagg tgttaggcat aaacttactg600aattttcccc attccttgat agtaattctt gtatggaatg tgaagcatcc aagctttctc660cgaaattgga gtcaactatt agtgtaagcc gacaccaacc ggttatttgt cagtgtcatt720taggcaccca acattcttta atatatcctt ctgatacaca ttccaacgta tcgagaactc780aaagtgtccc attttcgaga gattctacaa ctgatacatc caatgcacct agctgttctt840taagcccata taactcaaat gatggttcga gtaatcgatt tgtgaatgct gtaacctctt900ctttttcgat ggagacagat tttcaggatg cactttctta cgaaacagtt atgtttgctc960atcgcttcta caaacaactg tatcctcgca taattcgaga tatcgctcgc gttgaagaca1020actggagtcg tggtcagtat cacttaactc ggataccatg tcgaagtgat gttacaaaag1080
gcgtttattg tcttcaatat gataagcata aaatagttag cggtctaaga gatgatacta1140ttaaggtatg gcagcgaatt ccgaatattt gtacaatcaa taaaaacaaa ggatctggtt1200ccgactgtgc aactcccaat cttgatgaag ctggacagaa catccgagga agtgaggctt1260caacatcagc atcagctatt gaagctcaaa atcaagaaag ttttaatgca gcaacatgct1320ctgatggtta ccattgtaca caagtccctt gaagccattc aagctccgtt ttatgcttac1380agaatattgg g 1391<210>14<211>735<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>14ctgtcgctga agctacagat gatgatttga ttgaatttgt tgcaccacgc ctatctaaac60taattagtcc atgggatcat cttcttctac gttcggaaca tggtagtcct cctctccctc120aagttccacg tgtcgttcta gattatttga gtttagttga acgtgctcga tcattccttt180gtgatcaatt agagctacga ttaacaaaga aatcaaagat atcaaatgaa tcgaattctg240aaaattgtag agatggagat gataaagaca tgtcaaaaag caccttagat agtaaagagg300gtgaatctaa tgtacagtca aaagggaaat ttagttctca atcaaacggg ggggttcatg360atgatgacaa cgtagatgct gaggatgaag aagatgaaga tgatgatgat gatggggcta420tcataattaa ggtggataca ggtgaacagt cagctgcttt gggcttacca tgtggtaaat480atgtcgtaaa ggaaccactg cgtgaagaat atcttcctag acgtttccga tcgatattag540ctgctcaaag ggccaatgcg gcagctgctg ctgctggctc tgattccgaa aagaaagtgt600agggcatcag aaacgtgcag tagttgcatt ggaaacagct gaagatgatg aagtagacgc660aacttacgca gcgatatcac ctctacagaa gactcagtac aacatttgga gatgttacca720attcaaacca aactg 735<210>15<211>1235<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>15ccgtgtcctt aaatggtgat aatatttttt ccaagacatt gcttaaacgt tatgaacggt60ttatgtttcc tcgaaacgaa aatgaatccg atggtgaaat gcagataact caaacactcg120aatcaaaaga ttcaaacaat aaacatttga atgttgaaag caaccacgaa gatgataatc180tggctactaa agaatggcgt ttacgggata tggaaatgcg taaagcaata ctagcagcat240taacttctga acagcaatat cttccaaaag gacaattaca tccgcaaacc acagaagaat300ttgaattagc tgttcgtaat atgccatcaa atgaagcttg ttggattgct tatatgacac360atttgttgtc aggtaaaata ggtcaacaaa ctaataacag cggtctaaat aaaggggtta420ttgaagctag ggcaatcgct gaacgtggat tacgagctat ttcaaactct gccagtaata480atttactggt aaaacagtct agtttgctta tatcctattt aattatggaa gcaaaagaac540tggaacgtct cacttgtctt cagaaacaac aacaatatca tttatcctct tcttcaatat600ccgtgtcaga tctggtaatg gagattaata atcaatcaaa acgtgtttct caactactca660
cacaattact taatttagat caagcaccat ttattagaag agctatcgac actctttcag720atattggtca tcatacacgt gctgaagaat tagctcgtcg acagatcaaa tctcatccag780tcgatgtaga tcattggctt tcacttataa aagctcgatt ccgagctggt aatgttgagg840ctgctcggga agctcagcga aacgcagcct cattattaaa atcttctata cttccacaac900tcgccatcgg tgttgctaga atcgagtttg attatgggga tatagatcgt ggaatacgat960tgttgcaaga acagctaact attcatccaa aacgtaaact cttgtatgaa gaatgtatca1020aattgttatt attaaataac aagattaaag aagccagaac aattcaacag caagctgaga1080aaaatttaaa acctgtacaa tgttccttgt taaattcatt gtttaaaggt caggtgactt1140gttgattgta ttcaattgta tatcaaattc ccgatgttag aatgtttccc ctttttttac1200tattatcatt gggactgtga ataaaatttc tgtat 1235<210>16<211>469<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>16aacaaacgac gtaaatataa atcgaggaaa cggaattaca ataacaaaca ttatggatca60aacgatcatg aagaaaatga caatgaacat tactatagga aatcaaaaaa agatagaaag120aggaaaaaaa agtatacagg gcatgacgac gatgatgagg aggagggaca tcatgggaaa180tatgagtcga atagaaaacg gaaaaaagat cattccagga agaaatatgg ttataaatat240tctaaaaaga agtataatgg cgatgatgaa gacgatggat attaatctga aaaatgtaaa300gaacaaaaca cacggtaatc attactcaga tattataatt cataataaat ttactactga360aatggctttt atcgaaatgt taactgactg tttcttctcg tatatataag aaaaagaaga420attatgtaat caattgtcac ttagtcccca aaataaaaca ataatattt469<210>17<211>1058<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>17tgttgttgtt tatgaagata tctaactgga ataatgcaaa gaaataaaaa taataaccgt60ggaccattac gcgcgatttc gattgctgtt acaaataatt ctccgacatt accagtaaaa120agagcaaatg aatatggtgc aggctataaa caactaaacc ttacagtgcg aaaaacctag180aggcaggcgg cataccggaa tgggtcttcg cagttctgcc actactcaag tgcttcaaaa240tataaaacga aaatttagcc atttaaggag agcatttagt gcagaccgaa taaataaaaa300ttctacacca tccgacatta aagaatctta ttgccttgac tacgaatctc tcctaagaaa360tcccagtcga caattgatta ctgattcaag ttttaaacat gacagtatgg atcaacccta420caaacttact tgtaaaatta ttaataaaaa tcctgatggt tctattcaaa tttccctaag480acgctcatca attaatggac aatttggctt ttacattgct cgtgactcta aaggcatcta540tgtaagtcga cttggaagtg ttcggtgtgc agcaaaactc tgggatgttt ttcatgttgg600tgatagaatt ttagaagttc aaggtctgtc gtgtcataat ctcgacgttg aagaagttcg660taatcttata cggggttgtg aacttgttga gttcaaagtt aaaccatgcc atcacacaga720
ttccaatttt atacataaac actcattata acttagtctt tcttactttg ctttttttta780aaacatagat gacatgtaat tgtttttgcc ttcctgaaga attcaaaact atttatgtat840tgtaaaaaga ttcccagatt ttttaatatt ttttttcgat ataaaattgt acagtttaca900cacacttcgc acaaagatgt caatactact attgttatga tttcactgta tttactagat960ttcaatatat tattatttta ctataaagtg agataactta tgaggtttta aaaatacttt1020ttttgtattt gtacataaaa tgatttattc aatagcac1058<210>18<211>678<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>18cggttagtta tcgacgtctg ttaaaaatat atgactagct cattattgct caaatgcacg60cgatttgcat tttgactttt ggagtggaat gttaacacag caaaccaacc agacctcttc120tagtaatcga ggtcatcgag gttctggtac accttcacag aaaaaagacg aacgtcagca180ttcaggaaag caaaataata tgcaacaaga tgcaccgcgt tcttcacggg aaggaaatac240attgctggtt ggtccgaatt ttcgtgttgg aaagaagatt ggttgcggga atttcgggga300gcttcgtctc ggccgtaatc tgtataccaa tgaatatgtt gccattaaac tggaacctat360gaaaactcgt gctcctcaat tacatttgga atatcgattt tatcgtatac tactaagcgg420tggtgttgtt ggctttccaa tggtttatta ctttggacca gttggtcggc ataatgcact480tgttatggaa ttgcttgggc cgtctttaga agatctattt gaatcttgta aacgccaatt540ttcgctgaag actgtgctga tgattgcaat tcaactgctt cggcgtattg aatatgtgca600ttcaaagcat ttaatctatc gtgatgtgaa accagaaaat tttcttattg gacgtcagta660ctataataga catcgtac 678<210>19<211>678<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>19gaaaaatcgt aataatgatt actggaaatg aaagataggt tgcttgtatt gttacttctt60ggggttggag ttataaaagt gtagatattt tcatgtttag atgaagagta tgatgaagaa120gaaaaagaag aacgtgaccc acttgaacta ataaagcata agcataataa aagggatttg180gattcttttg aaaaaacatt gttaattcca ataccaataa aagaagaaat tatagaggct240acatctagag gtctgtctcc taaactccgc gttaagtgta aagaccttga agatgaatgt300tcttgcaaac gactcttgtg ttttggccca aacagctgcg aatcgatgag caattattcc360actgaaatga tgtcacataa ctccacggaa tttgtgtggt tacatcgttg tcgtcaaaca420tttttgatgg gacagaactt gtgcgagtgc aagcgattat tatttcataa gaaggatgct480aaattgctga ccccaaacac tgaaaacata cccttgatgg aaggagcaag ttcaggaatt540ggaagggcaa ctgctatctt gtttgcaaaa ctaggagcat gtgtcgcttt ggttgctcga600gataaatcgc gattgcaaga aactcgacaa gcatgtattc aagtatctca ccccgatgtt660tacgaaaagc ataaggag 678
<210>20<211>578<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>20atacattctg cttctagaat gaacaaggcg gtggttgtgt tcatgaaaag agctaatttg60gtcggtaggc taattgctag cggaatattt gtaagggatg tgttggtgtc aatttcacct120ctctctaccc cttcaacaag agttgtagtg gcaaatttgc ctccgtttat tacggatgac180caaattagga aagagctgag tcgttttggt aagtttgcta gcggtttccg tgtactgtca240gcaggttttc aggcagatgc cgttaagcac gttgtttcat tcaggaggca agtgtttatg300tttctgaaca ataatgagca acagctaaat gtgcatttta aagtgaggca tggggaaggg360ctctatgcag gttttgccag cacagatagt ctacggtgtt ttgaatgtgg ggatttgggg420cacaagagct ttgcgtgccc acataaaggc catagacaag gggagggtac gagcgcaagg480gggggaaatc gaggtgaaaa tgcagggggt aaggagatgc agacagcaga ggctgggcct540agtcagtcta gagatggtgg tgtagatgag gctgggtc578<210>21<211>744<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>21caacattttc tatataatca ttgggctacg gtgattgtat aagatggaaa tgaaaatgaa60gttgatgatt ttcctgataa tgttaatgat atcaatagtt tatacacaaa atggacgaca120tactctgagg ccacaattaa attccacaca tggtagtcag acagacaaaa atgatcggag180gagccagaga gataggttta gtgataagag aagtgaacat tcttcaaatc atgggaaaaa240taaaattagg acagaaagca tgttgggaac atcctcatct agatatcaat acagaaaagc300aagggaattc aatgcaagag gaaaatcccg atcacgcggt atcggaaaat atggtacaat360gtcatcagaa tcaacagatt tcgtagttga aggaaagatc ggtaaatatg gtcggaggaa420gcctactaag tctaaattca aaactcgtgg aaaagagaag aaatattcaa ggcgaatcaa480tgacaatcgg ttcgacatta agggtggttt gtatgaacga cgtcaacatg tgggtattgg540tgattatcaa gcgaatggta gtcacattga cgtcatgctt aaaactaagg aaaagagtgg600cagttcaaat ggaaccatga ttggcagaga ttcatcatca cattcaaata gtactcaacg660aaatgaacgt tcaatgtgga atattttttg aattgggttt gttattgtta agataatatt720ttactacatt gttcagataa taaa 744<210>22<211>1516<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>22caacttttga tcgatcagct gcatttgaaa atattcagta tgttaactcg cagcagattt60
ggagtttatg ccaatgaaaa tgccgcagtt ggtgttaaag atagctttgt acaaaaagcg120aaaatcatgg gtgtatcaac gaggtcacgc actgctctag gtgatatcaa gaacaaggcc180actagtatgc tagttaaaga tataggaaag ccaaaaagcg taaaagatga gcagttttcc240gaacagaaaa tggataaacg gctcgacatg agtatcgacg gcttaactcc tgatgtacct300actcaaacat atccacataa gctcaaagaa tttcaagcat gtcgtagaat cgtgagaatg360gattgcttag catcacagct ggctgaggaa ttcctagatg ttgaccgaaa cgattacgga420gatgcagcta cggtttatcc gtactctgaa cgaatattca attatttaca agatagagag480ttgttagtcc aacctcttgt agctgactac atggctccaa actgtgaaat aactccaaga540atgcgttaca tcttaataaa ctggcttgtt caagtacatt acagttataa actgcaacct600gaaacacttt atttatgtgt tgctattctc gaccgttatt tattgaaaaa ctcaaaaagt660ttaacgaagg atgggtttca gttaattgga gttgcatctc ttttcattgc tgctaaattt720gaagagatgt atccaccaga tataagtgat ttctcatcaa ttacaaacaa cacatattcg780aaaagtgata tacggaatac agagcaggtg atattgcagt caatcgattt ttacttgtct840attccaactc cattggtatt ccttcgtcgc ttgtcaaaag ctgtggatgc tgataggact900atgcacaatc tggctaaata tttcttagaa cttacagtac aagagtatga tctagcacat960cttccgggaa atctacgatc tgtgactgcg ttgtgcttgt cccgagcact atgtttaggc1020acttcggaac ttgaaagggc ttggtgtaat aaactgtcat atctcagtgg ttacgtgttg1080gatgatatcc gtgattatct gcagattctt gctcgcgccg cttatcgtca aaattctcct1140tcaaaatata gggcgatatt taacaaatat cgaatggatg atttttatgg acgtgtggcc1200tctttgccac agcttcgctc atatctcatg gagactttgg ctgatctcaa attcgatagc1260gatggcgagg caaatccagc ataatgctat ttgaaaaaac tgcatacaag tcattatcca1320gtgatacatc gtcacttaat tgtctatctg tgtctaggcc aatttatttt cccaattatt1380tatattctat cacctctgga gatttttgat tatctcattg gaataagtgt atattttagc1440ataatcgcct tctctatatt aagtgttagt gtataatctt ttaatacaca cttttatcaa1500aaaaaaaaaa aaaaaa1516<210>23<211>1653<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>23ctactttacg ttatacgaac ggttctgtac gcgggtgaat ttatcgtcgc atacggagtg60tgtaggacta ccgcgaagca tttcagcatc tgtttaggtt tatcagaccc agttacctct120aaggccatcg taaaaagttt caacaaccac gatcaaatac acagattcaa attcaaaaag180aaacaagatc ttcattggga aaaacgtcaa agattagtga aggtcactca atctgctacc240cttactactg agattggatg cctacgagcg ttaacaaatc taccttccca taggtgcttt300aatttctagg taagataatt catgtgtata ctttgatata ctgtagtact ggtataacaa360tatcgcagaa gcaactgctt ctgtgatttg atgtggctct ttcaacgcgt gcctaaacag420cagaaagaag aaagagtatt acgaacaaac tatacattta tttattcagt aggaagacgt480ctgctcagta atttccatgc tgttttttaa tatcattaac tcccattggt gtttagtata540gttaccagtt cagcttattg catcctgctg tatctattat ggattcacaa ggtttgcctc600ctggttggtc aattggttat gatagcatca ctggtttccc attttttatc aatcatattg660ataaatcaac tacatgggaa gatccacgga agtctaaggc taataatagc acacaaaatt720
tgcactctgt tccaacttat tcatcagaac ttactaacaa ccattatcaa cagcacaatc780tggaaaatga acgattaacc gactattctg acgactcgaa aacgggttcc attcgcaaca840atattctaca caaaaagcag tcgtattatg ataacgttag tgagccaata tcgatcaaag900tacactctat cgattctttt aataatgata ataataaaca tcacgaatat gaacatcgat960catgcacccc aatcactcaa tcatctgatt cgtataaatt caatcaaaac tcttttacca1020atgaaatgaa atcggttccc actgagttag tatctgttaa aaatcccgat aataataata1080tcggtgaaag tttatgcaaa ccagcgacat tagatccact cgctattatc gatcaagctc1140aatgtgagct taatgaaata aaagctgaga tcaacgagtt tattccttca tgtaaaaata1200aagcatattt attacttgaa gacaaacttg acaagctcat gttacgatgt gataacatag1260aatttgctgg tgatctaacc atcaggaaaa aacgccgtga agttatactg gctattcaaa1320cggtactgtt agatctggag aataaattgc cacagtccaa atcagtaaat gatcaagaca1380gcaagactaa tgatctccaa tcttctaata ttgataaagt tgataaaaaa gatcattcgt1440cattagattc cagtgaaact gatagcaact ttattacaga agaaggtcaa gctaaataga1500tcattctcta ataaatttta ttcgattttc gtagaagttt gatgtagtga cactttaatt1560tgctttataa tattttctca ttatcatgtg agtagttaaa ttactgtaca aaacataata1620tatcacattc atttcaaaaa aaaaaaaaaa aaa 1653<210>24<211>728<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>24cgcaatttcg taaacatgaa acctgagcag atttccatgc tcaagcagtt tgtcgagtta60cttaaaacaa aacctgaaat actcgacact cccgaactgt tattttttaa agaatggctt120gtgagtcttg gagccaatat tcccatttca caacctaacc gatcgactga atactccgtt180tcagacgatt ctggtgccga cgaaacctct gaatctgaaa tagaatttga tgatgatgat240aaagtactac caaaagagga tgttctagat ttggaaatgg gtgacaaaac gattgaagta300actgatgaaa tgatagagaa agctgatgaa aagaaaagtg aagccatgga gaaaatgtct360gaaggggctt ttggtgaagc tattaactta tttacagaag ccattaagct taatccacaa420gatgcattgc tttatgctcg acgtgctagc tgctttatta aaatgaaaaa gccgtctcat480gccatagcag attgtaatga agccatctcg ttgaatcctg acgttgcgca accctacaaa540tggagaggat ttgccaataa aatgattggc aattgggaaa atgcctattt ggacctgcaa600gcatccctga agttagacta tacagacgat gcttatgaag cagtaaaaga agtagaacca660aagcacaaac gaatattcga acataacatg aagtatatgc ataaacgaca agaaaaactg720gatcgaga 728<210>25<211>1171<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>25tcccactaga atgccttcgc aatattattt catatcagta tttttgatat gttttcattt60
gtctacacat gtgcatgcag tgatggacaa tcgaatgagg actcagttat tatctttaca120taatactgca cgagcagctg tacgaaatgg acaactcagt ggacaaccga tagccgtttc180tatgaaatta ttgaaatgga atacggaatt agaaatgaaa gcacaatttt tatctgatca240atgtcgcttt ggacatgata caaataatga tcgtaaaaca tcacaatttc catatgttgg300acagaattgg gcaggttcac aagatatcga aactggattt caactgtggt tagatgaata360caagtattat gatttcaaca ctggaacctg tcacttggct caatgtggtc attatactca420aattgtttgg gaaaatacta ctgatattgg atgtggtgtt agtaattgtc cgaatattcc480ttataaacta agtattgttt gcaattatgg tcctgcagga aatcatattg gacaagcacc540atacaagaca gcgactggag tgactgacag tgaaccacaa cagcaacaaa caaaacaaac600ggttacaagt ggatataaca gtaataacaa tagcagtaat aataatagca ataataacac660cagtagtaac aataatacca gtgatatcag tgataataac agtagtaata gtaatagtaa720taatgacatc attaataata ataataataa taataataat aataataatg gcaacagtaa780taatgatatt aatagtatta ataataacat tagtagtagt actagtaata gtgatattag840taataataat gacaataata ctgacaatga taatagtagt aataatatta aaaatgaagg900tgctgaaggt gagaaatatt atcttgatgg ttacaataat cggaaatcaa aatgcaacag960aaaatcacgt caaacttata ataaaccaat cactaataat caattaccat ccccttcatt1020atgctcatcc aattcgaatt catactatgg ttcaccaaaa ctaattcccg gtattatttc1080aaaagtagta aatctgttat gatccgtctt atttacaact tcttttctat aaacaatgaa1140ttcactgttt taaataataa ttataattaa a 1171<210>26<211>719<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>26gaaatcgcag cagcaagtat tttccacaca gtaatattcg aacaaacggg tcttatatgt60atgaggaatt tatgccaaca gatggtaccg acgtgaaggt ttacacggtc gcagatgatt120atgctcatgc ggaggctcga aagtcccctg ctttggatgg gaaagtggaa cgagatcatg180agggtaaaga agttcgctat cctgtaatac tgacacctag agaaaagatt attgcaaaga240aagtagccaa agctgtacgg cagcaaattt gtggatttga tcttcttcga gctaatggca300tgtcttatgt ttgtgatgta aatggatttt cttttgtgaa gtcttccaaa aagtactatg360atgactgcag tcatattctt ggtgtattaa taacgcgaaa aatagcacca cgcttatgtt420tgccgactaa tttgccacca ggtacagatg ttgacactcc actggtgcca acgacttgtg480gcgcaattat ggaactccgt tgtgtgattg ccgtcattcg ccatggtgac aggacaccaa540aacaaaagat gaaaatggaa gtttatcatc aaaagttttt cacatttttt accaaatacg600ctggtggctg ggctcgtgaa ctgaaaatca aacgtccttc tcaattgcaa gagattttag660acattgtgag atctattttg gaagaaattg attctggcaa tgtactgata atcgtcttc 719<210>27<211>935<212>DNA<213>日本血吸虫(Schistosoma japonicum)
<400>27atgcatccac tttgtacaaa tctactacaa cactcatcag tgagagggta gaggaaatcg60catcagaact ggaaatgacg agtgactgtg atttacttcc tatgtatgta aaatactggg120agttgtatca tcgcggtctt acatacctag acatccttta cagatatatg aatactcagc180acgtgaagaa cttgaggcca tcagaagctg atatgtgcta tggagcggca cttcctatgg240cagatcaaca cacaatggaa attctcgagg taggccttgc gttctggaga ttgtatttaa300tcgactacat aaaaactcga ctatctgcct gcctcatgcg agaagtcctt aatgacagac360tgggtatatg tggacaacat aatagcatac atccatgttt agcttctttc ttgagagttg420gggaactgag aaattttgat aaagctggga tggagatata caaccaaata tttcagaacc480ctttgaatga tgcgacgcga tcattttact ctcagtgggc atgtcaacgg gagtcggaat540taggatgtgc ccagtatgta actgaggcgc tagctctgcg gacagaagaa cacaatcgtg600ccatgcaata ttacaaatgt tcaatactac caatacaaag tttgtttcaa gagataattg660ttgagcagcg tctaaatttt cttaacatga atattagaca tgttattgct gaagaagata720agtcaaattt gaggaatctt ttccggttgt tatctccaaa taatctttgt agtgagttac780tgcattgttt tggtgaacat gttcggacta gcattttaga agctatctcc aatcttccaa840aagactcctc attaacacag gttcatttta tagagagtct tttaagtctg cgaagcagat900ttttggatta tatcgatgat gttttcgatg gaaaa 935<210>28<211>1134<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>28tggacgtgat ggtgaacctg gtcctagagg tttcccaggt tctaaaggtc aagatggctc60tcagggttta cgtggttcaa tcggaccaag aggtccttct gggaaggacg gagatgatgg120tgaaatgggt gcagctggac ctccaggacc tgcaggtcca cctggtccat ccggaccacc180gggtgtacgt ggtggccgag gttcatctgg tgaagccggt gatccgggtc cttcaggacc240acaaggtagc cgtggtccaa ctggtgctcg tggacgtcgt gggaaagtcg gggttgctgg300tggtcctgga agcattggtg ctactggaga aagaggtcca ccaggtccaa ttggaccatc360aggccagcct ggacctattg gccagccagg tcaacctggt ggtaaaggtc ccactggtcg420agacggaaag ctaggtacag atggaaaacc agggacacaa ggacaagttg gtttaccagg480tcctattggt gaacgtggcg ctgctggtga agatggtgaa caaggttatc ctggtttaca540aggtccgaaa ggtgaagttg gttcacaagg tattcctgga cgtattggac cagctggtcc600accaggtgtt cgcggtggtt caggccaaat gggtttaaga ggaacaaaag gtccacgtgg660tccaccagga gaggacggac caccagggaa agacagttta gccggtgaac cagggccacc720tggtgaacca ggtccacgag gcccaataca agttcagggc tatgacaatg gtaaagttgt780tggtccacaa ggcgctaagg gcttaccagg atatcctgga ccacgaggaa gaccaggaat840accaggtgca cccggtgatc caggtccgat tggagagtct ggagtaccgg gggaagatgg900accaccaggt cctgatggtc ctcgtggaaa agatggaatc gatggggccc cgggtcgacc960aggaaaatca ggcaagtctg gtcaatcggg agctgttgga gcccagggac cgcctggaaa1020acctggagat gctggatacg tttggtccgc caggaccttc tggaatccct ggtccatctg1080gagaacctgg tctacctgga attgatggta ttaacggacc gctggacctc aagg 1134
<210>29<211>927<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>29cggcgccttt gattttgtct ccgttatcac gcactgcaag catgacacaa ggttttttta60gtaatccttc tgctggaact aatgctaaag gcaaagagag tcaatacgag gataaaggga120agcctgctgc tatcagatca agtaacatag ttgccgctaa agctgttgcc gatgccattc180gtactagctt aggacccaaa ggaatggaca aaatgataca agaggcaaaa ggagacgtca240ctatcactaa tgatggggcc actattttga aacaaatgaa actgcttcat cctgcagcca300agatgttggt agagctgtca aaagcacaag atattgaggc cggcgatggg accacttctg360ttgttgtcct agcggggagt cttcttgatg cttgctcaaa actattggac aaaggtatac420acccaacgtc catctcggat gctttccaaa aagctgcatt aaaagcttgt gatattttgg480aaggaatgtc tatccccgta gacttaggaa accatgatga acttatcaaa gtggcgacga540cgtcattaaa ttccaaagtt gtctcacaac attcagattc tctagcaccc atagccgtca600aatctgtttt ggatgttatc gacccggcac atcctaactc cgtctctcta gatgatatac660gtgtggtaaa gaaacttggg ggcacagtcg aagacactaa gttagtcgat ggtcttatac720tcacccaacg ggctgagtct tgcatggtgg agtcaaaacg agtagagaaa gcgaaaatag780ctctttattc aggttctgta ttctctcctc caaaaacaga tatggaaaat aaatgttgtc840attacggatt atacttcaga tgggatagaa taatggaaag aagaaagaca ttacattcct900taacattatt aaagctgtaa aaaggct927<210>30<211>812<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>30gatggagttt cgggaggtgc ttgctttggc gaagaaaaaa cagtccaggc acgataatga60ggtcaaacgc atcgaagaac acgcaaaaca acgtgaaatc gaaaagaaaa aacagatgga120gattgcagac gaactgagtc gcaaatatac taaacacgct actgtgacta acaacaatgt180aaacatttcc aaccttggtg ccttcagttc acctaccacc ttgccaactg tcaaagcttc240tacagtaaga tctgaaacat ttagctcaaa ctcaagttca gtgtgcaaat ctaacattac300agcaatccat aaggttacgg accatgaaaa agtcgttaaa ctacctaata gatcattaga360aagtcctatt gtgccatgct ctgtaaagcg tgccaaagtc ccacctaaac ccagtcgcct420ttcatttaca gatatgatgg aactggctaa aaaaaacgta cagcacagcc cagatagaaa480aagaccattt gatgatctta ttccttgctc tgtagattca aaatatagaa aactagattc540tagctcaact gaaggtctac gcactcctct agtcacgcct aaaatcacta agaagcccaa600gctgtcgtcg aagacaaaag ttccagaatc tttaaaggtc actccgaatt tacaatcgaa660aatttccatc tcaggtggta caaacggttt ttgtaaaact gcaaatagga gtgtaattga720aaaagctgtt cagcatcttg aaaaaccgat caagttctca acgtgcttga agaaagaacg780gtctaatcac actcgtgtga atctgcctac ct 812
<210>31<211>386<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>31MDYNTGIAVLTGGNHIYVTTSIETPRMRRLVELPGPVKSLSQWIIVTTPLETGSPDSFRS60PWVLFADKKNLYRADFGTLENIELSKLIKSTGVNISLLSISLNMQFVAIYLSNGELLIAN120TRFTELHSHINLTQRISVMLMSNNNNNNINNNNNTNNSSSNQKLNHPKTMLWLTNSAVTL180QWNNLVAIISTQSDIYETFYADDIYLAQEVDGIRIISPTSHELLQRVPSPLEALGRIGAS240CPATWLLSASKSLKANCGRTNDYLLPIKQTNQMIDAVSHCIEAACHATVDPICQQELLES300AHLGRILLSAMSSTTPTTTNVTNVNSIDNYSVEVKVKELADHAAKVCQTLRLLNNLAVPW360LGIALTWRQFRELGPNKLFERLLARC 386<210>32<211>204<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>32MELEGVDSFISSYVDSLQILPLEMQSRITEYLQSDLQQRHLLALAKQTMGKLQKSSEYNL60KRKHLEQLICVLVAIQNICDRRISGLDELDEVIETHSLKLMNAKLKVDAISSQLTHPNTE120NVPESEVDISDVEVSGHQSNGSFLRRNLTQELNFDKCTANSMPRTRCNDFSLLSKERFPL180RAVSTNNRTATRRSLNRWVSGVHR204<210>33<211>248<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>33PPVRVPHYKMREIVHLQVGQCGNQIGSKFWEVISDEHGIDPSGEYRGDSDLQLERINVYY60NEAIGGKYVPRAILVDLEPGTMDSVRTGPFGKLFRPDNFVFGQSGAGNNWAKGHYTEGAE120LVDSVLDVTRREAENTDSLQGFQLTHSLGGGTGSGLGTLLISKIREEYPDRIINTFSVVP180SPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTNPTYGDLNHLVSA240TMSGVTHV248<210>34<211>281<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>34MTEVDIKIIKRTQETLGKLIKKPVLTEKLLQRPPFRFLHDICTAIIRSTGLMKGLYTAEE60
LEADNVKDKNKKLAFLQKLVDFLAVVHGRPIPVRIMSIVAGKEAEKTNEMLYLLADAVNK120KVNNQECVAHVLRGNEKDNTKANNTIKKPGPENDQLKGKVNVTPNSMDPERRLYPQENNL180SSEKANTSENRLSIDDDKSFYSVKNSSGSVNSEKCDTTKKNRLSQDSGSVGGNRCDFQKP240EKLQKEVVVSLPSSSTI PHQPRHHRPASAKGQRVKQDGKLF 281<210>35<211>220<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>35QIDVIKRRSEKLNHELRRQLSRFI IRGQNGDVESVAQLRKNSVHSSSSSLCSSHINSNNN 60NNTVSVTSTSDEIMSDLKVNGKVSNVTMSKGSFNEMPLGFFSTADFKAIIDRMSEVQEEN120CTLRRYKKRLEADLLTKSQI IQNGLDNYLNSYSFTVKQSTSNDSNTSLSTTTTTTTTSSS 180SFPLSTTSYSSSMITNNNTFPNTTYGFHSKISLQILIHHH220<210>36<211>260<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>36SSPLPIHSKKNNYDLMNRKLVKHKDLLSRSLKLPTSNEHECKLKQYELCIDGHREIIQGI60HTTTSTTLSTTMTMNNTNSSLPTGSEMHESKFYNQPENTESTCVFGRVIVEIIKEEKSNL120GLTVSDGSNQSEAPVILNIRPGSIADRSDCFLPYDHILSMNFTTASSDNTTSSKHFASKI180QMEIGYELPALPPVGCTVKHMVVNLKIGSDGVGLVIRGGWNKSPLLIRPLTVMHIRQNST240ADCDGRIKPGDRILTINNIH260<210>37<211>416<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>37LVTRYGMVSLKRIACDDVASKSTVCKHSKKSKSIDCLSTEVSENGIDFNKSHQTWNFKKG60DFKDEMPNHKSKKKLLKNSISELTNGIPKLLITSLPCHMSTTALKDIFPTVVNLKMRKTA120GSKHAVLDFSCNEDYLDAVNRLKSIEFDGVKPNYRAVVGHTENAIQSNLSKQFVNNNPNC180LILRNLPYSLTATEIKEHFPLADRIHLDVNKFGIFNGSCILVMKSKEDLEIVLDKCKHKY240INNRLVRVNLQSESKLESSKHHDSTNMGYGLKLKEVPNIIEDEHIKALFPQTSLIGLSSR300IFQDSNTRNVFIFFKKNSYRKSALKMFKTEVVLGHPISCRLWVYFSKICIPVPSNRRCKS360QTAKINMEVNNTVLNNKMPKPNEFSKNEKKLNLYECRNASSSSSIPKKIVFDSDNE416<210>38<211>270
<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>38MMLIPPNPLNSQTACEPFISYGISPGFVKQVREKFQSEISMQELSKNLQPRVKHHSATAR60LTCAYENLKHNDSGVQRHNTYPNRRIQSSGRVSDTLPSTKLTKYLETKNHADTLKIDKKC120PTLSVGLSENVKSNPAIHTNDYRPQTAVNKYQLDNNNGYSNTPSYVSGTVHNRSHPNDQN180VSKYNETDNKPKEPDVFLATKYNTRNDFKQPAVLPKPQKCEFTKDSLINLIVPRGFEARK240QLNQHTYNEKEHFRTVKSENIPNKNLKSRS 270<210>39<211>117<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>39MFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKKVYEPMFNVQNKNPSYFVDWIPKNVKTA60VFDKPPRGLKMSVTFIGKSPPIQKLFKGGSEPFTAMFCRKAFLHWDPGEGMGEMEFP 117<210>40<211>243<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>40MDTQRTKYSCLTKRLSRRLSRLSRNCQDATEEFSILLQDLIKLLDQHNVKVSPDSSESVL60SLNLIADMLDENVFRLLSEFHHTSWKILTALTQPYQPVENSSNASSLSKCNFPAEGSVEK120YLNQPLDEEYFMRHLFSSTKLFCMLETNVGALEAGLMSQISYLEGTYTANESIPPNSNKE180CLKQLEVECTKLASELATLNQSKVMQLKSRNELIEKQNQLKHMNDIEKILRQENSWVAVF240DKL 243<210>41<211>154<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>41EQAELEEEQKELDKEAAVLERRLRQQMTYEPGTALEEELYKRWFILVSKKNALIHRGFQL60SIMEKEDDLKKKMNMLQDELRSILSIEEYLKTDNDRKREDLLLQDLVRLVNERDDMIQEL120DLHEKALSEEMDLGQNTSFVPHGRLKVDKSCVLQ 154<210>42<211>282<212>PRT<213>日本血吸虫(Schistosoma japonicum)
<400>42MLGSFSQTKQIPISSDHVRVIASSCIGFIVVASEATIDGFPQSDDEKRTKKLELVNSLER60KLCLSSSAKRDEKWTFTQSQGIALLIPLKHIPTVLINSETIQSGFCELLGRFIKRLCIHE120NPAICQIGYRCVGCFISHLTANHDVTYEPKALLELLGKGFEHSVIDMRMLSTVVSNHIAW180HVKLPMPSWISTFVNILLAGTKDKNSPVRLGSETALAVLCRISAPKSNKDKCPNSGYLQA240CYDALDNNTRNQLETLVQRLRKQNWSEVWRQGCPDMDNTNSL 282<210>43<211>397<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>43MEKKNCVDTFTGWSSGDQVDFICNLLSKISHFQQSQINDFLEPLLKRDFIVALQNRGVPY60LAEMILVQLDASSLLSVELASRGWQWSVAKYQLWRRLLARRVATDPVWHGLCERRGWIEF120IEHSDPAYLPALLERRRVPLGVRHKLTEFSPFLDSNSCMECEASKLSPKLESTISVSRHQ180PVICQCHLGTQHSLIYPSDTHSNVSRTQSVPFSRDSTTDTSNAPSCSLSPYNSNDGSSNR240FVNAVTSSFSMETDFQDALSYETVMFAHRFYKQLYPRIIRDIARVEDNWSRGQYHLTRIP300CRSDVTKGVYCLQYDKHKIVSGLRDDTIKVWQRIPNICTINKNKGSGSDCATPNLDEAGQ360NIRGSEASTSASAIEAQNQESFNAATCSDGYHCTQVP 397<210>44<211>199<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>44VAEATDDDLIEFVAPRLSKLISPWDHLLLRSEHGSPPLPQVPRVVLDYLSLVERARSFLC60DQLELRLTKKSKISNESNSENCRDGDDKDMSKSTLDSKEGESNVQSKGKFSSQSNGGVHD120DDNVDAEDEEDEDDDDDGAIIIKVDTGEQSAALGLPCGKYVVKEPLREEYLPRRFRSILA180AQRANAAAAAAGSDSEKKV 199<210>45<211>380<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>45VSLNGDNIFSKTLLKRYERFMFPRNENESDGEMQITQTLESKDSNNKHLNVESNHEDDNL60ATKEWRLRDMEMRKAILAALTSEQQYLPKGQLHPQTTEEFELAVRNMPSNEACWIAYMTH120LLSGKIGQQTNNSGLNKGVIEARAIAERGLRAISNSASNNLLVKQSSLLISYLIMEAKEL180ERLTCLQKQQQYHLSSSSISVSDLVMEINNQSKRVSQLLTQLLNLDQAPFIRRAIDTLSD240IGHHTRAEELARRQIKSHPVDVDHWLSLIKARFRAGNVEAAREAQRNAASLLKSSILPQL300AIGVARIEFDYGDIDRGIRLLQEQLTIHPKRKLLYEECIKLLLLNNKIKEARTIQQQAEK360NLKPVQCSLLNSLFKGQVTC380
<210>46<211>118<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>46MDQTIMKKMTMNITIGNQKKIERGKKSIQGMTTMMRRRDIMGNMSRIENGKKIIPGRNMV60INILKRSIMAMMKTMDINLKNVKNKTHGNHYSDIIIHNKFTTEMAFIEMLTDCFFSYI 118<210>47<211>183<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>47MGLRSSATTQVLQNIKRKFSHLRRAFSADRINKNSTPSDIKESYCLDYESLLRNPSRQLI60TDSSFKHDSMDQPYKLTCKIINKNPDGSIQISLRRSSINGQFGFYIARDSKGIYVSRLGS120VRCAAKLWDVFHVGDRILEVQGLSCHNLDVEEVRNLIRGCELVEFKVKPCHHTDSNFIHK180HSL 183<210>48<211>196<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>48MLTQQTNQTSSSNRGHRGSGTPSQKKDERQHSGKQNNMQQDAPRSSREGNTLLVGPNFRV60GKKIGCGNFGELRLGRNLYTNEYVAIKLEPMKTRAPQLHLEYRFYRILLSGGVVGFPMVY120YFGPVGRHNALVMELLGPSLEDLFESCKRQFSLKTVLMIAIQLLRRIEYVHSKHLIYRDV180KPENFLIGRQYYNRHR196<210>49<211>111<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>49MSNYSTEMMSHNSTEFVWLHRCRQTFLMGQNLCECKRLLFHKKDAKLLTPNTENIPLMEG60ASSGIGRATAILFAKLGACVALVARDKSRLQETRQACIQVSHPDVYEKHKE 111<210>50<211>192<212>PRT<213>日本血吸虫(Schistosoma japonicum)
<400>50IHSASRMNKAVVVFMKRANLVGRLIASGIFVRDVLVSISPLSTPSTRVVVANLPPFITDD60QIRKELSRFGKFASGFRVLSAGFQADAVKHVVSFRRQVFMFLNNNEQQLNVHFKVRHGEG120LYAGFASTDSLRCFECGDLGHKSFACPHKGHRQGEGTSARGGNRGENAGGKEMQTAEAGP180SQSRDGGVDEAG192<210>51<211>215<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>51MEMKMKLMIFLIMLMISIVYTQNGRHTLRPQLNSTHGSQTDKNDRRSQRDRFSDKRSEHS60SNHGKNKIRTESMLGTSSSRYQYRKAREFNARGKSRSRGIGKYGTMSSESTDFVVEGKIG120KYGRRKPTKSKFKTRGKEKKYSRRINDNRFDIKGGLYERRQHVGIGDYQANGSHIDVMLK180TKEKSGSSNGTMIGRDSSSHSNSTQRNERSMWNIF 215<210>52<211>414<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>52MLTRSRFGVYANENAAVGVKDSFVQKAKIMGVSTRSRTALGDIKNKATSMLVKDIGKPKS60VKDEQFSEQKMDKRLDMSIDGLTPDVPTQTYPHKLKEFQACRRIVRMDCLASQLAEEFLD120VDRNDYGDAATVYPYSERIFNYLQDRELLVQPLVADYMAPNCEITPRMRYILINWLVQVH180YSYKLQPETLYLCVAILDRYLLKNSKSLTKDGFQLIGVASLFIAAKFEEMYPPDISDFSS240ITNNTYSKSDIRNTEQVILQSIDFYLSIPTPLVFLRRLSKAVDADRTMHNLAKYFLELTV300QEYDLAHLPGNLRSVTALCLSRALCLGTSELERAWCNKLSYLSGYVLDDIRDYLQILARA360AYRQNSPSKYRAIFNKYRMDDFYGRVASLPQLRSYLMETLADLKFDSDGEANPA 414<210>53<211>306<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>53MDSQGLPPGWSIGYDSITGFPFFINHIDKSTTWEDPRKSKANNSTQNLHSVPTYSSELTN60NHYQQHNLENERLTDYSDDSKTGSIRNNILHKKQSYYDNVSEPISIKVHSIDSFNNDNNK120HHEYEHRSCTPITQSSDSYKFNQNSFTNEMKSVPTELVSVKNPDNNNIGESLCKPATLDP180LAIIDQAQCELNEIKAEINEFIPSCKNKAYLLLEDKLDKLMLRCDNIEFAGDLTIRKKRR240EVILAIQTVLLDLENKLPQSKSVNDQDSKTNDLQSSNIDKVDKKDHSSLDSSETDSNFIT300EEGQAK 306
<210>54<211>242<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>54RNFVNMKPEQISMLKQFVELLKTKPEILDTPELLFFKEWLVSLGANIPISQPNRSTEYSV60SDDSGADETSESEIEFDDDDKVLPKEDVLDLEMGDKTIEVTDEMIEKADEKKSEAMEKMS120EGAFGEAINLFTEAIKLNPQDALLYARRASCFIKMKKPSHAIADCNEAISLNPDVAQPYK180WRGFANKMIGNWENAYLDLQASLKLDYTDDAYEAVKEVEPKHKRIFEHNMKYMHKRQEKL240DR 242<210>55<211>363<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>55MPSQYYFISVFLICFHLSTHVHAVMDNRMRTQLLSLHNTARAAVRNGQLSGQPIAVSMKL60LKWNTELEMKAQFLSDQCRFGHDTNNDRKTSQFPYVGQNWAGSQDIETGFQLWLDEYKYY120DFNTGTCHLAQCGHYTQIVWENTTDIGCGVSNCPNIPYKLSIVCNYGPAGNHIGQAPYKT180ATGVTDSEPQQQQTKQTVTSGYNSNNNSSNNNSNNNTSSNNNTSDISDNNSSNSNSNNDI240INNNNNNNNNNNNGNSNNDINSINNNISSSTSNSDISNNNDNNTDNDNSSNNIKNEGAEG300EKYYLDGYNNRKSKCNRKSRQTYNKPITNNQLPSPSLCSSNSNSYYGSPKLIPGIISKVV360NLL 363<210>56<211>239<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>56NRSSKYFPHSNIRTNGSYMYEEFMPTDGTDVKVYTVADDYAHAEARKSPALDGKVERDHE60GKEVRYPVILTPREKIIAKKVAKAVRQQICGFDLLRANGMSYVCDVNGFSFVKSSKKYYD120DCSHILGVLITRKIAPRLCLPTNLPPGTDVDTPLVPTTCGAIMELRCVIAVIRHGDRTPK180QKMKMEVYHQKFFTFFTKYAGGWARELKIKRPSQLQEILDIVRSILEEIDSGNVLIIVF 239<210>57<211>311<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>57ASTLYKSTTTLISERVEEIASELEMTSDCDLLPMYVKYWELYHRGLTYLDILYRYMNTQH60
VKNLRPSEADMCYGAALPMADQHTMEILEVGLAFWRLYLIDYIKTRLSACLMREVLNDRL120GICGQHNSIHPCLASFLRVGELRNFDKAGMEIYNQIFQNPLNDATRSFYSQWACQRESEL180GCAQYVTEALALRTEEHNRAMQYYKCSILPIQSLFQEIIVEQRLNFLNMNIRHVIAEEDK240SNLRNLFRLLSPNNLCSELLHCFGEHVRTSILEAISNLPKDSSLTQVHFIESLLSLRSRF300LDYIDDVFDGK 311<210>58<211>367<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>58GRDGEPGPRGFPGSKGQDGSQGLRGSIGPRGPSGKDGDDGEMGAAGPPGPAGPPGPSGPP60GVRGGRGSSGEAGDPGPSGPQGSRGPTGARGRRGKVGVAGGPGSIGATGERGPPGPIGPS120GQPGPIGQPGQPGGKGPTGRDGKLGTDGKPGTQGQVGLPGPIGERGAAGEDGEQGYPGLQ180GPKGEVGSQGIPGRIGPAGPPGVRGGSGQMGLRGTKGPRGPPGEDGPPGKDSLAGEPGPP240GEPGPRGPIQVQGYDNGKVVGPQGAKGLPGYPGPRGRPGIPGAPGDPGPIGESGVPGEDG300PPGPDGPRGKDGIDGAPGRPGKSGKSGQSGAVGAQGPPGKPGDAGYVWSARTFWNPWSIW360RTWSTWN 367<210>59<211>305<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>59APLILSPLSRTASMTQGFFSNPSAGTNAKGKESQYEDKGKPAAIRSSNIVAAKAVADAIR60TSLGPKGMDKMIQEAKGDVTITNDGATILKQMKLLHPAAKMLVELSKAQDIEAGDGTTSV120VVLAGSLLDACSKLLDKGIHPTSISDAFQKAALKACDILEGMSIPVDLGNHDELIKVATT180SLNSKVVSQHSDSLAPIAVKSVLDVIDPAHPNSVSLDDIRVVKKLGGTVEDTKLVDGLIL240TQRAESCMVESKRVEKAKIALYSGSVFSPPKTDMENKCCHYGLYFRWDRIMERRKTLHSL300TLLKL 305<210>60<211>270<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>60MEFREVLALAKKKQSRHDNEVKRIEEHAKQREIEKKKQMEIADELSRKYTKHATVTNNNV60NISNLGAFSSPTTLPTVKASTVRSETFSSNSSSVCKSNITAIHKVTDHEKVVKLPNRSLE120SPIVPCSVKRAKVPPKPSRLSFTDMMELAKKNVQHSPDRKRPFDDLIPCSVDSKYRKLDS180SSTEGLRTPLVTPKITKKPKLSSKTKVPESLKVTPNLQSKISISGGTNGFCKTANRSVIE240KAVQHLEKPIKFSTCLKKERSNHTRVNLPT 270
权利要求
1.一类日本血吸虫表皮特异基因，其编码具有日本血吸虫表皮特异蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.30所示的序列；(b)SEQ ID No.1～SEQ ID No.30所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.30所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的一类日本血吸虫表皮特异基因的核苷酸序列，其特征在于所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.30所示的序列。
3.一类日本血吸虫表皮特异蛋白，包括(a)具有SEQ ID No.31～SEQ ID No.60所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
4.如权利要求3所述的日本血吸虫表皮特异蛋白，其特征在于，其具有SEQ IDNo.31～SEQ ID No.60所示的氨基酸序列。
5.一类抗体，其特征在于，所述抗体能与权利要求3所述的蛋白特异性结合。
6.权利要求3所述的日本血吸虫表皮特异蛋白在制备血吸虫疫苗中的应用。
7.权利要求3所述的日本血吸虫表皮特异蛋白在制备防治血吸虫病的药物中的应用。
全文摘要
本发明公开了一类日本血吸虫表皮特异基因及其编码蛋白，还公开了与该日本血吸虫表皮特异蛋白特异结合的抗体。此外，本发明还公开了此类日本血吸虫表皮特异蛋白在制备血吸虫疫苗中的应用，以及在制备防治血吸虫病的药物中的应用。运用本发明的日本血吸虫表皮特异蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物过程中发挥重要作用。
文档编号C07K14/435GK1986804SQ20051011172
公开日2007年6月27日 申请日期2005年12月20日 优先权日2005年12月20日
发明者刘锋, 韩泽广, 王志勤, 胡薇, 冯正 申请人:上海人类基因组研究中心, 中国疾病预防控制中心寄生虫病预防控制所