日本血吸虫雌虫特异基因、其编码蛋白及应用的制作方法

专利名称：日本血吸虫雌虫特异基因、其编码蛋白及应用的制作方法
技术领域：
本发明涉及生物基因领域，尤其涉及日本血吸虫雌性成虫特异蛋白质的基因核酸序列、以及特异蛋白质的氨基酸序列；此外，本发明还涉及日本血吸虫雌性成虫特异蛋白的应用。
背景技术：
血吸虫病由血吸虫的感染引起，在世界上有76个国家，受感染者约1.5亿。致病的血吸虫有3种，既日本血吸虫(Schistosoma japonicum)，曼氏血吸虫(S.mansoni)和埃及血吸虫(S.haematobium)。在我国流行的是日本血吸虫。血吸虫病的流行给人民身体健康和经济发展都造成巨大的损失，因此，防治血吸虫病、开发研制治疗血吸虫病和抗血吸虫的药物具有巨大的社会和经济效益。
研制新的治疗血吸虫病和抗血吸虫的药物，首先必须找到合适的药物作用靶点。血吸虫的虫卵是造成血吸虫病的最主要原因，肉芽肿就是由虫卵造成的。此外，虫卵排出体外进入外界环境，污染环境后会制造更多的感染。所以，治疗血吸虫病、防止血吸虫的扩散，一个关键的措施是抑制血吸虫雌虫成虫的产卵。这可以通过干扰雌虫性成熟，干扰雌、雄虫的配对，或者抑制产卵相关的生理途径来实现。而雌虫特异表达的蛋白质，很可能与雌虫性成熟、雌雄虫的配对，或者产卵密切相关。通过大规模的基因表达谱学研究，发现了日本血吸虫雌性成虫的特异蛋白质，这些蛋白质，都是可能的药物作用靶点和候选疫苗。
在本发明之前，还没有出现涉及本发明的日本血吸虫雌性成虫特异蛋白的公开报道。

发明内容
本发明要解决的技术问题之一是提供一类日本血吸虫雌性成虫特异蛋白，及其基因的核酸序列。
本发明要解决的技术问题之二是提供一类日本血吸虫病防治疫苗，该疫苗以日本血吸虫雌性成虫特异蛋白为免疫原。
本发明要解决的技术问题之三是提供一类与日本血吸虫雌性成虫特异蛋白结合的抗体。
本发明要解决的技术问题之四是提供日本血吸虫雌性成虫特异蛋白在制备预防和治疗日本血吸虫病的药物中的应用。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明的一个方面，提供了一类日本血吸虫雌性成虫特异蛋白，包括(a)具有SEQ ID No.24～SEQ ID No.46所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
较佳地，所述蛋白是具有SEQ ID No.24～SEQ ID No.46氨基酸序列的蛋白。
在本发明的另一方面，提供了一类编码日本血吸虫雌性成虫特异蛋白的基因，包括(a)SEQ ID No.1～SEQ ID No.23所示的核苷酸序列；(b)SEQ ID No.1～SEQ ID No.23所示的核苷酸序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.23所示的核苷酸序列中每条序列有至少70％同源性、且编码相同功能蛋白质的核苷酸序列。
较佳地，所述基因具有SEQ ID No.1～SEQ ID No.23所示的核苷酸序列。
在本发明的另一方面，还提供了一类日本血吸虫病防治疫苗，该疫苗以日本血吸虫雌性成虫特异蛋白为免疫原。
在本发明的另一方面，还提供了一类与日本血吸虫雌性成虫特异蛋白结合的抗体。
此外，本发明还提供了日本血吸虫雌性成虫特异蛋白在制备预防和治疗日本血吸虫病的药物中的应用。
在本发明中，术语“日本血吸虫雌性成虫特异蛋白”指具有日本血吸虫雌性成虫特异蛋白活性的SEQ ID No.24～SEQ ID No.46所示氨基酸序列的蛋白质。该术语还包括具有与日本血吸虫雌性成虫特异蛋白质相同功能的、SEQ ID No.24～SEQ IDNo.46序列的变异形式。这些变异形式包括(但并不限于)若干个(通常为1-50个，较佳地1-30个，更佳地1-20个，最佳地1-10个)氨基酸的缺失、插入和/或取代，以及在C末端和/或N末端添加一个或数个(通常为20个以内，较佳地为10个以内，更佳地为5个以内)氨基酸。例如，在本领域中，用性能相近或相似的氨基酸进行取代时，通常不会改变蛋白质的功能。又比如，在C末端和/或N末端添加一个或数个氨基酸通常也不会改变蛋白质的功能。该术语还包括日本血吸虫雌性成虫特异蛋白的活性片段和活性衍生物。
在本发明中，术语“编码日本血吸虫雌性成虫特异蛋白的基因序列”指编码具有日本血吸虫雌性成虫特异蛋白活性的核苷酸序列，如SEQ ID No.1～SEQ ID No.23所示的核苷酸序列及其简并序列。该简并序列是指，位于SEQ ID No.1～SEQ ID No.23序列中，有一个或多个密码子被编码相同氨基酸的简并密码子所取代后而产生的序列。由于密码子的简并性，所以与SEQ ID No.1～SEQ ID No.23所示核苷酸序列的同源性低至约70％的简并序列也能编码出SEQ ID No.24～SEQ ID No.46所示的氨基酸序列。
在本发明中，可以使用一系列本领域已知的方法来制备针对日本血吸虫雌性成虫特异蛋白的抗体。例如，将提纯的日本血吸虫雌性成虫特异蛋白产物或它的抗原片段注射入动物体内以产生多克隆抗体。同样，表达日本血吸虫雌性成虫特异蛋白或它的抗原片段的细胞也可以用来对动物致免疫而产生抗体。本发明制备的抗体也可以是单克隆抗体，这些单克隆抗体可用杂交瘤技术制备。
应用本发明的日本血吸虫雌性成虫特异蛋白，可抑制血吸虫雌性成虫的产卵，从而治疗血吸虫病、防止血吸虫的扩散，该类蛋白在血吸虫病防治和治疗中有着重要的作用。


图1是用RT-PCR实验验证日本血吸虫成虫特异表达基因的图；图2是图1的数据分析图。
具体实施例方式
下面结合附图和实施例对本发明作进一步详细的说明。
以下实施例仅用于说明本发明而不用于限制本发明的范围。实施例中未注明具体条件的实验方法，通常按照常规条件，例如Sambrook等人，分子克隆实验室手册(New YorkCold Spring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1日本血吸虫雌性成虫特异蛋白的生物信息学分析。
1.日本血吸虫雌性成虫特异蛋白的筛选利用表达序列标签(EST)的数据，分析特异表达的蛋白。雌虫相对于雄虫特异表达的基因1)其差异显著性必须满足p＜0.05，这个显著性分析用网上专门的软件工具计算获得；2)每个基因，其EST数量必须大于等于10条；3)雌虫的EST数量必须是雄虫数量的3倍或3倍以上。经过这3个标准的筛选，得到了雌性成虫特异表达基因。
2.同源性和相似性分析日本血吸虫雌性成虫特异蛋白序列对GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余数据库(Non-redundant，NR)做BLASTP比对；日本血吸虫雌性成虫特异蛋白序列对Swissprot数据库做BLASTP同源性比对。根据比对结果得到的与已知其他物种或已知的血吸虫蛋白的序列相似性，做出日本血吸虫雌性成虫特异蛋白的功能注释。
实施例2RT-PCR实验验证日本血吸虫雌性成虫特异蛋白的表达情况。
1.样品准备准备日本血吸虫的成年雌虫和雄虫，以抽提其RNA。
2.总RNA抽提试剂盒抽提RNA试剂采用TRIzol reagent(GIBCO/BRL)，该试剂基于酸性酚一步抽提法生产。用于抽提RNA所用器皿和水均要进行无RNA酶处理，以保证实验中无RNA酶环境。
3.RNA抽提步骤将碾杵和匀浆器等器皿在200℃干烤4h，去除RNA酶，冷却；加入液氮中预冷，将组织从液氮中迅速取出，碾成粉末；用刮匙将组织放入预先加入TRIzol试剂匀浆器中，匀浆数分钟；将匀浆后液体转入无RNA酶离管中，加入氯仿后，4℃离分层；将上层水相转入一无RNA酶离管中，加入氯仿后，4℃离分层；将上层水相转入一无RNA酶离管中，加异丙醇，4℃离沉淀RNA；用75％乙醇洗涤沉淀2次；用无RNA酶去离子水溶解沉淀。抽提RNA质量鉴定紫外分光光度计测定260/20比值(比值均在1.7~2.0)；并在MOPS甲醛变性胶中观察有无降解。
4.RT-PCR反应用半定量反转录多聚酶链式反应(RT-PCR)来验证日本血吸虫雌性成虫中一些基因的表达丰度差异。采用不同的循环数，分别是21、24、27和30个反应循环。每个PCR重复3次。实验结果见图1和图2所示。
序列表<110>上海人类基因组研究中心<120>日本血吸虫雌虫特异基因、其编码蛋白及应用<130>NP-10284<160>46<170>PatentIn version 3.3<210>1<211>1732<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>1atttaatcga catattagtg atctagactt ttatgttaat tatgttgacg gttattcaac60aagttttcag gggccaaact ataatttaag ccatatcaca acaaaaaatg tttcaatggc120tgtcaaacgg tatccattag ctgagcctcg tggtatcaaa gcacatttca ttatggatca180caatggaatt cttgttctta gtggtgttag ttgtctattc catcctattg aaaaaaatga240agtatctgac aattcaacaa aggaaaaatc tgcttttgaa aaaatcggga gtactttaag300tggattattt ggtggtggta gcagtagtag tggtaatgac aaaatatctc cggaacaaga360tacacctatc aacacaacaa acaatagtga cgttaatcca actggtgcaa attcagtaaa420ttttactaat aactctgctt tttcagaaaa tcaaactaga tctcatgttg acgataatgt480ttctggtgaa gatcaggcta cagtggttgt aaagtctacg gtaccatttt ctgaacctat540tgacagtgaa gttgtctatg tagattttcc tgatttaact tccaagcagt ttacactgtc600acaatctaaa ttacacgaat tacgtgaagc ggatagagcg aaagctgctt tagagcagtc660gattaatcaa cttgaaacat tattgattga tacacatgat aagatactaa ctgattataa720aatgtatggt actgaaagtg aactttcaca gttggataat acattacaaa gtgcttccag780ttggttttat gaacaaggtc atgatggttt aaaaagtgac tacgatttga ggataaggga840aattcaatcg ttgcttaatc ccattgaaag aagatttaat gaagttaaat atcgtgagaa900agcaatctta tcatttgata aaacgattgg attagctcag aaaacattaa atgaaatgat960tgagttatcg caattgtttt caaggacatt ggaaaccatt tctcctgaac tgcaacaaca1020aaaaccttca acttctacct ctgaacaagc aacaaaggaa tcaccttgga aaaacatttt1080caatgaggct gaaattgata tactaagaaa taaaattaat gaatcaaaga tttggttagc1140cgaatcaagc gaagctctaa acaaatctct aacataccag gatcccccag ttttagtttc1200tgccatccaa agcaaagagg ctgaattatc ccgagagact gtctatcaca ccacaaagat1260taatttatgg agatcagaaa ttgcccgtat gtttgctgaa caaagcaaaa taaatgctac1320atctgttcca ctaaaagaag aagctcaaac accaccaagt agtagtgatg aggagaccat1380tcctaaacca tcaactatca acgaagatca ggagcctaca ctaaaaccaa cacagacacc1440aagaccggaa ttgtgaatac aggatttatt cccaacatat tatgaaatga actcaactag1500agaaaggatg ataaattcac agttcataat agtctgttaa attaaaattt atagtttgct1560
taatttacat attcacttga aaatctgtac tgtgatgtct tctttactgt ttgtgtaaat1620gtacttcttc cattcatttt tctccttatc tttgtgtttc caatttaaat aattttatga1680gtagtattgt aagcattaat tgaattgcta atatatgtat tttttatagc gt1732<210>2<211>930<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>2gtttaaaggg aacaaagttg ggttcccccg cgggggcggc cctttaaaaa ttggtgctcc60cccgggcccc aggatttggc caggaggcgc ggccgtttga taaatcgaaa agacttgttt120atggccgtgt taagagtgtt gtgcggactt ctgctggttt ccattttgtt tcttgggttt180gtcctcagtg aagcaaatgg gccgaaagta acagaaaaag tgttctttga tattgaagtt240gataaacaac ctctcggtcg aataatcatc ggattatttg gtaaaacagt accgaagact300gtggaaaatt tcaagcaact ttctattggt acaacgttga aagatggtcg aactgccgca360tataaaggaa gcacttttca tcgtgtcata aagtcattta tgatacaggg tggtgatttt420acaaatcatg atggaactgg cggctttagt atttatggtg agcggtttcc cgatgagaat480ttcaaactaa aacatgttgg tgcagggtgg ctgtcgatgg ctaatgctgg tcctaacaca540aatggagccc aatttttcat cacgactaca aaaaccccgt ggttggatgg aaaacatgtt600gttttcggaa aagttgtaga agggatgagt gttgttagac aaattgaaaa catgcagacg660gactctagag ataggcctgt taaaagtgtg aagatagcaa actgtggaca cattcctgtt720gatgtaccgt tttcagtatc caacactgac gctgttgaat gaactcggat gaagaatttt780ctattttctt gtatttatat ttatacgtaa atataattta ggataatggg aaaaaccaat840aatatgattt attgacggaa ttggacgtaa aacccccgag ggggggccgg tacccaatct900cgcccttaag ggcggcctat acacacttcc 930<210>3<211>892<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>3cccaatacaa cgtaccgtca ggatagacgc aaagcgatct gaggctgaaa ttcacaatat60agcatgctta cttgttcgat ggccttactc tcttaatgaa atgaacttat tatatagatt120gattttatgt gtaacaactt ccaagttatt caattgagat ggctcttaat caactgtcgt180ttttagtaga taaagcaaaa aaagagtatc catcaagtct gaaattttta atgtgttgct240ctgcagcatt tgtttccgaa acgatcactt acccgctcga catgcttaaa actcgcactc300aggtatacgg ggaattacag tcttcatcat atcctggtgt agttagaatt tgttcctcga360taattaagaa tgaaggtttg tttcaacttt ggcaaggatt atctccggcg cttatcagac420acataattta tacaggagcc agaatgccaa tttatgaaat cattcgtcag gatattttcc480acttaccacc tgctagtcat ttcactgtaa agtcctctaa gaatttctat tcagaaacca540actcatctat taccctacat aataaattac ctgccactaa aactacagac tatgaattaa600tgcaggaaaa caaatcggat tttattattc gagcagcact tacaggtatg atagctggtt660
cgtttgctca gttcttagcc agtcccattg atttaattaa agttcggctg caaacagagc720gcaaatggat gtcagaaata catcccaaca attataaacc ttctaagcct actgaagtac780ccgtacgcta tcagtaaacg tcttagtttt atacgtacaa ctaagcaact tatttctgaa840ggtggcatca tcggtttatg gcgtggagga ttacctaatg tacaacgtgc ag892<210>4<211>782<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>4acataatttg tttcacgaca tactggtcag tcaattcaag ttcagcccct ctacgtattt60gcttgtttat caaatcagcc gataccttgt catctgaaca taatgaagat aaacattgat120ggcctactgg tctattttcc gtatgaatat atatatccag aacagtacca ctacatgatt180gagttgaaac gtactttaga tgctaaaggt catggtgtct tggaaatgcc atctgggact240gggaaaacag tctcattact ctcattaata gttgcctata tgaaagctcg tcctggaata300gttgaaaaat ttgtttattg ttcacgtaca gtacctgaat tagagaaagt cattgatgaa360ttaaaagtcc ttgataaata ttatgcagat gaaactaatg aaaaaggatg tggtctttta420ggaattattc ttagttcgcg taaaaatcta tgcattcacc gcgatgtaaa acatgctggt480gatggagctg ctgtagattc cgcctgcttt cgactaactg ctagttttgt acgtaaaaat540cgtatagctg atcctaatgt tgcgtactgt aagtactatg aggaattcga tttaaacggc600cgggataatc cttttgcccc ggggatttac agtatggcag atatcaaagc ttatgctaaa660aacataacta ttgcccttac tttcttacaa aaatgctatt tcctatgcaa atattatcgt720ctacagctac ttttacctac tcgatccaaa atagcaaatg tcgctctcgg aattacccaa780aa 782<210>5<211>1789<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>5gtacgtcagc atggcggtag tgtgacttag ttggcacagt aggatgatgt ggttgtcgtt60gttgttcatg ttagtggcac cgtttgtggc tgctaatgag gacaagcgga aaaatttact120tgtacttttg gacgatgttt atgtcaagga aactcattct acatttttaa aaatgctaac180aagtaggggc tacaacttga ctacaaaact tgctgatgac tcatctcttt cgctgaaaca240ccttggagaa tatttgtact caggcttaat aattttagcc ccatctgtca gtgagttcgg300cggtacgata aatgttaatg agataactaa tttcgtcgat gatggaggta acctcgttgt360agttgctggg cctaatgtcg gcgagtctat tcgtgagttg gggagtgatt gtggtgtaga420attcgacgaa gaaaatacct tcgttatcga ccatttcaat tttgacaaaa aagatagtgg480tttgcactct ttaattactg tacctgttag taatttgatc aaaagcaaag taataactgg540aggaaaagtc aatgcaccaa tcttatatcg tggtataggt atttcagctg atcaaactaa600ccccttgctt attgacgtct tacacggatc aaagacatcc tacagttaca atccagataa660atcagtaaca gattacccaa atacagtcgg gacaaacacc cagttaatca ctgcattaca720
agccagaaat aatgcacgtg ccttattcat tggttcgtta gattttctgt caaactcgtt780tttcgaatct accgtggaga ctgagtctaa aatgagtgtt ctatgtggca atcaggtgct840tactgaaaat ttgatgcgat gggttttagg tgaatgtggt cagctacggt acacttctgt900aaagcatcat cgggtagatg agactgtccc tcctagccaa tatacaatca tggatgatgt960tgtttacact atcaagatag agacaaaaga tgatagcgga aattggatcc cttatgatgc1020agacgatgtc cagcttgagt tcgtacgaat cgatccattt attagaaaga acatggagca1080caaagatggt gaatacaaac ttgtcatgaa attacccgat gtttatggag tatttaaatt1140tgtagttgat tattatcgtg ttggttacac acacctttta agcgtcgtac aagtgcctgt1200tcgtccattt actcatacac aatatgaaag attcctcgtg gctgcttatc cttattatac1260tagtgcttta tcaatgatgg ttggcctagt gctatttagt ttcgtatttt tatacttaaa1320agatgataaa gaaaagggtg aataagatgc tggccatcac tttgtgatat attcatacat1380atatttctga tattcgtttt aacacaatct tatgagtatt gtatggtaat ttatatactg1440tccagttttt gctgtcttgt atgttaggta cttattttgt gttctaattt gcatcagctc1500ttaattcttt ttgttaacct acactacact ttttttcatg tttgtatttt gtgtacatgt1560gtgttgagtt agaagtttgt tttcattaca ttgatttgaa ttgtattaat ttttaaagtt1620taatcaagtt atgtaaacaa cagtagcaaa aatagtgtat aataatttag tatgaaaaac1680taattccaaa atgttatttt tactcagtgc ttaaatgttt catacttgtt tgctaattgt1740tttgacacat aataaaaaat actttactac agtaaaaaaa aacaaaaac1789<210>6<211>843<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>6cccactgcct ctgtggggag gaatgggaga ggaggcccca attcgcaaac cccctctttc60cgccggggtt ttggccgttt cttttaacgc aagcgcgcca ggaccggttt tccggtttag120aaaagggggg caggacgcgc ccccccaatt aggggggggt tggctcccct cttaggcccc180cccggggttt acccttttat gtttccggtc tcgtagtttg tgggggactt ggagggggat240acaattttcc cccggggaac aggttatccc ctggtttacc ccaagctgga atttacccct300cattagaggg acctggcctt gccgtaagtc gtttcccagc cattctaaat atgccacgga360gaggagcaag tccttcaaga gcaccgtctc gtccttatgc aagtctacct gcacgcacac420ctcagtctgc tgtcgcacat ccaccagcac aaccgcggca accaggtttg atgggtcaaa480tggcggctac ggccggtggc gttgctgttg gtcacgtagt tggcagcgct atcacaggag540ccttctcagg tggggctgga agcagccctg cacaatcttc tgcacctgct gtgccgtcgg600atcagtacca aagtccttgt caattccaag tagatgagct aatcaaatgt gcacaaactc660aaagtgacat tagcgcttgt agtggcttct ctgaagcctt gaaggagtgc agtcgtcact720atggattata ccagtaattt cgtgaatctg gaataactca gttgtgtagt ttcacgtcaa780atagatatat ttgattgtgt taactttgct ttttggtttg aaataaatta attactatta840gca 843<210>7<211>1327<212>DNA
<213>日本血吸虫(Schistosoma japonicum)<400>7aattcgcggc cgcgtcgacg aaacacaatg ggcctactcc ccctgaatga aacatgatgg60gcctactccc cctgaatgaa acacaatggg cctactcccc ctgaatgaaa cacaatgggc120ctactccccc tgaatgaaac acaatgggcc tactccccct gaatgaaaca caatgggcct180accccccact gaatgaaaca caatgggcct actccccctg aatgaaacac aatgggccta240ctccccctga atgaaacaca atgggcctac cccccactga atgaaacaca atgggcctac300tccccctgga tgaaacacaa tgggcctacc ccccactgaa tgaaacacaa tgggcctact360ccccctgcga gtagttcatt gctatcatta ttattcggtt accttttatg caattctctg420ttggttggtg gaaatgcgaa ccgatactat tctagtcaag cagtacccta taagcagcag480acaaatcagt actatggata taataatgaa ggaggcagtg atgatacata tggaaatgat540aattacgata aaaatacaaa taagtactct actaataaca atgacaatta taataattat600ggtgacaata ataactacgg cagtaacgat tacaacgcaa aatcatatga taactacaat660ggtaataaca ataataatga ctacaactcc gactacacac caaccaaaga caattatgaa720gcgaataagt ataaagaaga atacacacca agtaatgaat ttgttccacc aggtggttct780attgatgata gttctatgcc atcatttact acaggaacaa tatccggttc caaacatagt840catggtcgtt tagatgtcag aggtaaaatg gctggtgttg gacatcaaga ttatggtgta900aattatttat cgttgacaaa attccgtaaa ggtggaggtt atgatatata tggtaaaaaa960cggcaatttc tagattacga tacgcaagga catgtgaaaa agtatggtaa tcaacatatg1020agaacgaaat ttgaagttgt tggcaactta aatggatatt ccacgtataa acagaaatct1080tcttttactc cacccagtgg tggctatagt agcaaacgtg gcaggagatc atactgaaaa1140aagaaaactt aatgaataac cattacttta ttgtcattaa taaagtcatt actcctgatt1200ataaaaaata tatttgtttt cattaaaaaa ataaatattt aatgcggcaa aaaaaaaaaa1260aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa1320aaaaaaa 1327<210>8<211>1795<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>8cgaggtccac ttcgtctcct atttcaccga aatgttttct tgttcctaac tagctactca60gcactgttgt aagccttcat tattatatgc aagtacttaa atcgtaagtt attcaagttt120atcaatatca aaaaacaact gagtagtttg ctgtcacagt aagaaggtgc ttcttctaat180gttcatctaa cacgttaagc tctttctcat tcaatcgaaa tgtgtaaatg tttagatgtc240gtccatcaat aaactaaaca tatcaaatta ttttgcgctc aatgagaaga atcattattt300ttctttattt tgtgcagttt tttggacagt ggcgttacaa gctatgcata tcaatataca360taattacatc aatctttttc atcatagcgt atttgtcagg ttctccaagt cgaattgtat420attcccaccc tgatgaactt atcgatgatg cactctgtag tctgccagaa cctgaagttt480gttgtcctaa caagactgat cttgatctgt ctcaatttag ttctatctct ccgacagata540ttttgaacat tttaaacgtc actcagcacg tttgtgatgg aagttggaag ccgaaatgtt600tgttttcaca aaaaatagct atcatcatac cttacaggga cagagaaaaa catcttaagt660
tactattacc tagactgcat gccctaatgc ttcgacaaaa tatgccatat tatgttttcg720tgatcgagca ggctgggacc acccctttca atcgtggact gttacttaat gttggcgtct780tatacgcact ggagattgat ccagaggtta actgttttgt ttttcatgac gttgatcttc840taccggaaaa aagcgaaaat ctttacttgt gtgatacaga gctaagacat ttgtcaccag900ccattgatga gtttcggtat catccaccat ttataaacta tgcaggtggc gttgctgcaa960tgtcgaagga aaatattttc aagattaatg gatttccaac acgtcattgg ggttggggaa1020gtgaagatga tgaattttcc gctcgtggtc taattttcaa tctgaaatta actagaccac1080ctgaacatat cggacgatat aaagcaccaa gacatagaaa aggttccata tcatttggac1140atcaaagcgc atttttaaaa tttcaaaatt atttacatga tggtttaaca ctattaagag1200ataactttta tccaaaatgg aaagtgcatt cattaaataa tagaataaat aaattttcat1260tgaataattt agaaaaattt aaaattcctg cctttgtaaa ccaatgtacg cgcgaaaata1320ttttatctca attaaatctt gataagaatt caactgaaat tgttcatgac cttgtaaaga1380tatcttcata taatatgaaa ttattaaatc acttttcacg tgataatctc tttaagcatt1440ttatatttga tccaactgac ttatataatc aaagtgaatt catattgaaa ccacaaggta1500atcatacgga tgaaacagta tggtggttcc ttcactttta tggatggatt taatgtaatc1560attgatgctt tattcgtatg taggtttgat tattgtttat gttgtacagt attcattgtt1620tctttatgat tgtcttcttc agagaaatgg catataaagc aaaaagacaa aaaagcatag1680ttcaatatct tcaatataaa gtcctcaatg ttctctttgt catgttacaa ctttatttgc1740tatcaattat ttttattttt attatatatg acaaaataaa atacactttt ttttc 1795<210>9<211>1619<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>9cactaccatt accaccgccg ccgccaacaa caacaataaa gaaaggaatt gattctagat60gacattgttt aatataaaac aattaatagt aatcatctac ataattagtt tatctcaatg120tttaggttat attgtgttaa tgtcaagtga aggttataga tatggacatc aattacaaaa180tccattacca tattatacta atgaagaaga cattatttca agtaatgatg atgcatctat240atatgatggt gaaagtggag agtatattga ttatcatgta tcaccatatg aaaatgttat300tgatgatact actattcctt atcaaaatga tgaatatgaa gaaggtgaag cgtattcaca360agattatcca aatgtagatt atcaaactta taatgatgag aatagtcaat attatggacc420agttgaatat aataatatgc aatatgatca atcaagttat gattataata tgccatataa480tgcatattat tcaagaagag atacaaatga ttattatcaa gaaagacctg atatacaata540tcgtgttagt cttggtaata gaccatatca atatcaagaa gattatgatg atgatgacgc600tgctgattac tatagtaatg aaggtaatta tgaagatgat tataacagta ataatgataa660ttataaaagt caatctgata gtaatgtaaa ttttcgccag tattacaaat tgcctacagc720atcatataaa catcaaaagt atgtaccgca tagattagat acattgaaag attatcaacc780aattgatgat aaattatatg tattctatcc aaaagatata aaaccacaat attatacatt840acgtaaaatg catacaaaat atggtaagct aaatccatat ttctcatcaa gaataatatc900aaaaaaatca ccaaatggat actttaaaaa tgttttacca actacatatc aatctgatga960tcatcataaa catagagata attctatgca tagcaacaaa aataatcatt attattacaa1020atcaataaat aaatcaccta attataaatc gattgataat tattcaccaa ctagcaacta1080
taaagatcat catttaaatg gtagatcatc atcttattat cgtaacaata ataagaatga1140atatagacca tcaaaaagta aagtatcata tcctactact aataataaga agaataaacc1200attacgtgat gtttatggta attcaatgag aacaacagat tatgaagaga caatttcata1260tcataaaagt ggtgaaaaag gtcctaaggg taaaatgaaa agttattctt cctatgacat1320ttacggtcat ttaaaacata ctggtaaatc aaaattaggc agtggtatta cacgttatca1380tacagtgatt gatagtgata aaagtaaaaa tagacattat gttacttatc aaccattaaa1440taatgaatat aataaataaa tatttataga ctgaattaac cattgacaat agtattacgt1500aataataatt gtcaataaga acatttatta agaaaataaa caacaacaaa aaaacaacaa1560tgaaagatta caaatcattc taaagtattc cattacttat tgatcgttat ttagattgt 1619<210>10<211>609<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>10attaaataat acacatagaa gttatgatac gtctatctac ttcagtatta ttcatcatat60tgggaataat ctattcactg aaagcaaacg aaaactatcc atcaaaacaa acagctgatg120gtagttggtg tgatgataac actgaacatg actactgttc accttacttt gtcatctgta180caacaaaaca atatacgcga cgttgtttga gcaagtatac atttggtgga gaaggtcccg240aatatgagaa caaggagaat ataacattta ctgaacaatt aggcgaaaat ataactaatc300cattgcaatt taccatgcct gaatggtcta atgatacagt aatacatgta gctgtattca360ataaaggcct taatgttgtt tcattattgg gtcgaagtga catattgata gattggatag420aaacacctgg caccaatgaa agtgaaaaat ggcaagaagt atattttaca gcagatgaga480gcgaaatggc attagatgca tacgtcaaag tatttacttc ataaatacga taatacatgg540aatgtccacc ttcctaatgc atattaacaa tataaattac aatatcaaat atacactata600actaaatgt609<210>11<211>894<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>11taaaatcttc tactttctta ataagtggat tgacgtaaga atttatatga attgtactta60aagattttgt atagtttaat ttatttaatg tatcggttac gttggtgaat agaggatcag120tttaagtacg aaaataataa ggatttgata tcttgatttg tctctttgga tcataagcgt180gttggtgttg tttatattat tcttggagtg tggggtggat ttataggttt aggtttaagt240ttgttgatac gtttaaattt ttgtgatcct tattataaac ttattccttg tgaggtatat300aattatttga taactaatca cggtatagca atgatttttt tttttttaat gcctgtttta360ataggtggtt ttggtaaata tcttcttccg tttttttttg agtatgagtg atttagcttt420gcctcgttta aattctttga gtgtttgaat gatggttcct tcaatatttt atatggaatt480gagtttgtat tatggatctg gtgttggttg gaccttttat ccacctttgt cttctttagc540tacttctggt gttggtgtag attacttaat gttctcttta catcttgctg gtgtatctag600
tttggattgg ttctataaat tttattacta ctataatgtt gcgtctaagg tcatgttctt660cagttattag atgatcttat ttatttacct tcggtgttgt tattgttatc gttgccggtt720cttgctgcag gtataactat gttgttgttt gatcgtaaat ttggtactgc tttttttgag780ccagcaggtg gtggtgatcc tgtgttattt caacatttat tttggttttt tggtcaccca840gaagtatatg ttttgatatg cctggatttg ggatagaaac caaaaatgat gtct 894<210>12<211>868<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>12caattgttaa cacaaatctc agtaaacaat tatctgctga aatgggcgga cttctcacaa60cgattaaaag ttattttagc aaaatggaag cccgtattct aatggttgga ttagatgctg120ctggcaagac aacaattttg tacaagctga aacttgggga agttgttaca actatcccta180caattggttt caacgtagaa acagtggaat ataaaaaaat tagctttacg gtatgggatg240ttgggggtca agataagatt cgtcctttat ggagacatta tttccaaaat acacagggtt300taatctttgt tgtcgatagt aacgatagtg aacgtattgg ggaagcagca gacgagttgc360atcgtatgtt aggcgaagat gagctgcgga atgcagtact tttggtcttt gcaaacaaac420aagatcttcc acaagctctg tcacctgctg atattacaga taaactgagg ctacgttcat480tacacaatcg cgattggtat attcaggcaa catgtgcaac tagtggttca ggtttagaag540agggtttcga atggctcagt catacaatta gccaaaagaa atattaagct tttgcatttt600cactcagtac tgtgaagcaa cggcgatcgg cattaatctt ttgttccacg ccactggttt660ctcctaaatc tgttgtgtat atttaatgtg gtcatttgaa tccgtcctta cagtaataga720ctaatggatc tcggtccagt tgtcgctttc atttttttaa tttgttaatg tttttatgca780gaaaagctga cgtattaggt ctgatttatg ttgaacttca tcttccgtta cctgattgca840aacttggtta ataaagcccc attctttc 868<210>13<211>555<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>13agcgcttaat ttgattaagg acgtgctgca atgagattgt tagttaggtc acaccttgga60ggctcgatcg tagtatctgc aggaccaaat gaatccgtta agtcactaaa agataagata120tcggtagcca ctggcattca tgcatctaca cagtctcttt actatcatgg tttaccgctt180cttgatagtt ctcttctgga agaactcgat tttactgatc tggataatgt tgatgtgaat240tgcgaacttc taggtggtgg gaaaaaaaga aaaaagaaaa attacactac tcctaggaag300atcaagcaca aaaagaagaa ggtaaagctc gcaacgctca agttttacaa ggttgactcg360aatggcaaga taacacgact acgaagagag tgccccagtg aaaagtgtgg tgcgggagtt420ttcatggcta gccattttga ccgtgaatac tgtggaaaat gtgcccttac atataagcat480gtaaacaagt aaaataaagt aagaaacttc ggaaaaaaaa aaaaaaaaaa aaaaacacac540aaacaaacaa aaaac 555
<210>14<211>1445<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>14ctggtatccc cggtactgga aaaactgctt cagttcaagc cgtattatct actatgcata60aattagttgc tgattcatgt ttagaaagtc aattacctgt atttcaaaca atttatgtta120atggcatgcg tgtcagtgat cctaaacaga tttacatcca gatatatgaa caattaactg180gtttgattgc aactacaaag tctgcgtgtg atttattaga aaaggaattt tgttctagta240caaataaaaa attaaatcat cgtgaagttt ctgagaaacc tgttatttta gtcatcgatg300aattggattt attatgtact cgtcgacaag atatacttta cagccttttc gatgggccca360cgcgccataa taaccgtcgt gttttgatag tattagccat tgctaacact atggatctac420cggaacgttt gcttcatcca cgagtagcaa gtcgtttagg cttaactaga cttactttcg480caccttattc ccatgaacaa ctttctcaga ttgtgagaca tcgtttatca tctttgtcaa540acatacttca gcctaaagca cttgaattag ctgcacgtaa agtggcggct gtctctggtg600atgttcgacg cgctcttgat atatgcaaac gagctgctga aatagtttca tctagtgaaa660agactaacaa agaaatagat atttcgcata taaatgctgc tctaaaagag atgttcgtta720cacccaaatc agatgcaata tgtgcctgtt cattgtacga aaaattgttt cttcgtgctg780ttatagccga atttcaggcg cgttcaacag aagaagctcg attggatcga tgtattcgtc840aaatgtcagc actttgtcga cttgaaggtg ttccatgccc aactacatca gaagtatttg900ctatttgtgc tagtcttggg gctcacaaat tattgctgac ggaaaggtcg cgatatgata960ttgctatgct tgttcgtctc aattgtacaa agtctgatat tttatactgc ttaaattcac1020aatcaaatgg ataaatgcaa gataaatggc tttgaagatt tacccattca tttgtacttg1080agactatatt gtttttcatt tcaaaaacca tacatggata tgtgtgcatt tagataaatg1140tttcgcattt tgtttaattt ctgttgtctc aaaccttact attcatggaa atcgaaattt1200ttaaatgttt ttataaatca ctttgtcaat aaattgcaag attactattt ttgtattatt1260tctattatat ttgtagataa aaactgattc ctatgtattt taaaatgctt cctctatatt1320tcttaaagtc actttctatt gcatctttta gaaatcttct tttattcatg atttattgat1380attctagggt agcttttaca atataaaatt aatattctat tgaatacatt atctttctaa1440tagat1445<210>15<211>495<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>15agaatcgatg ggatggtatc gctaaaagtt gacaacctgg cttataggac gactattgat60gacctgcgaa gagttttcag ccgatttggt gaagtcggtg atatctacat acctagggat120ccatatacat tcgaaagtcg ggggttcgct ttcgttcgct actgcactga tcgcgaggca180gattgtgcta tacggggtat ggatggccat aaagtagacg gcagagaagt tcgggttcag240agagcaaaat atggaagacc aacacccaat cgcagaagac gatcggttac tccgcgacgt300aatggacggc gttctcgttc tttttcatct ggtcgtgaca ggcggtcacc gcgtagaata360
aacgaccgat atcgtcttcc ttcctctcct cgacattata gtcgatcgtt gtcaagagaa420cgacgttaac tgatgtcatt tatttaaatt gttcattgat ttaacgcgta catatacatc480atgaatttat tatct 495<210>16<211>707<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>16atatttttgc tttcataaca tttatttgta gtaataaagc tgacaaattt cccactaaga60atattcttac agaaagttat aatgatgact acaaaggttt atacaatgaa aaacatcatg120atcgaaaaga ccctcaggat tattggaaac ccaatcatga ggataaaaag aactatgaat180ctaatggtta tggagagaat ggcaacaaat atgtagatga aggtcatgga aaagacagct240accctggcta cccttcaagt ccccatgaaa catataaacc taataaacga gatgataaaa300gaaaatatga tgttcatagc tatgacgatc attacgacta tttcaaagat ttcgataagc360atttgtatgg cgacaatcac aacaatcaac cttcacatga taagtatcca gaaaaaactt420atcaaaattc tgatgagcct cacacagacg actatgatgg taatcagata aagcctgaat480ataataatta tcaaaatgaa tataacgaac attatcaacc acaacaaaat gattactata540ctaattcagg ttatggttcg gaaggtgact actattatgt ttagagccta cggcatcatg600aaaactgaga aagaatttga gtattgaaat aaatgaaagt gtgtatatat aataagataa660tgtattctta tttgaggtta tgcaatatga agctcctaat gaattgc 707<210>17<211>925<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>17gttgtccaca cggtcgacct acactcatcg tgttgtacaa tgagaccatt tgttatcggg60ttcttgttct ccctcatatt aataattagg agtagtcaga tagatcaaag taatactgta120ttcattggtt caataaccgc tcgcgacaag gaccatttcc gtgcagtttt cgctaaacat180ctcagtgaat tgccgtccga agctttcata atttaccatg ctatactcgg ttcccactgc240ctagatgtaa aggtccccaa catagattca atctgttcta cacttaataa acctgtaact300aacgccgaag aatcattcta tgcaagttcg atctacaagc tcacaggttc ttcaaagtgc360aaggtatcaa ctgcagaggt cgaaactctg tccaagcagc tgttggtcga agatatacca420atcgaaagtt tattttatct aatttcatcg atgaaaaact tggatattaa aattgatgct480aaccgtgttt caacaatatt aggcaaaata aaagccaaag ataccagccc aatgacgtta540agcttcatgt tgcatattct atcacaactt gggttgacaa aatcagcttt aagtggctat600tcatctagtg taggtgatgt tttggatcaa gcaggtgaaa taagcgataa ccagctgttt660tacattaaag gcttatatac atcagcattt gttgcaaaaa gcgttgttga atttcttaca720gcatacggcg aagtcccaaa aggcgttgag aataaattag tcaaactgtt caattatctt780tacacacgtc gtcaaaccac caatatacgt gcttccgcat atttggttgc tgctttcaaa840tcattagctt atagctcatt actgatgcct gttgtaatcg aatcatcagt taaaactaat900
gaagattaag gtctctccat tcccc 925<210>18<211>870<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>18gcatgggagg aatatgatat cgctatcaaa aaaggtggct atagttacag gagcaagttc60aggaattgga agggcaactg ctatcttgtt tgcaaaacta ggagcatgtg ttgctttggt120tgctcgagat aaatcgcgat tgcaagaaac tcgacaagca tgtattcaag tatctcaccc180cgatgtttac gaaaagcata aggagccatt tttgtgcatt gaagctgatc taggtgattc240acatgaagtt gaaaaggctt atcgactggc tttaaatcac ttccatcaac ttgatatatt300ggtaaataat gcaggattta tgatacaaga cactgtagag acctttaata tagatgaata360tgaacgcttg atgaaattga acctaactgc agcaattact ttaacaaatc ttgctgttcc420tgatcttacc actaccaaag gttctatcgt aaatgtgtcc agtgtttgtg gcgaacgttc480attcccggga gtaatgtctt actgtattag caaagcagca ttggatcaat tcactaagtg540cactgcttta gatttagctt cgaaaggaat ccgagtaaac agtgtcaacc cagcagttat600tgttactgaa ctgcataagc gagctggaat gtcagaagac gattataaaa agtttctttt660aaaatctaaa gaatcacatg cactcggtcg aactggaact gtggaagaag tggctcaagc720aattgctttt ctttcaagtt ctgcatcttc cttcactacc ggtaatttat taatggttga780tggtggtcga tctatcatgt gcccacggta gagttttttt ttctacatat tataagttat840ttcttaaata aagtggatca ctggaaatgt 870<210>19<211> 1851<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>19acattcgtaa tcgagattcc tatgacgttt ttattcttca tgccttttcg aacaacacgt60ctcgcatcat tttattcgca ggtacttctt caacttgcga taattttgac cggaaactac120aatttcttca atttgttaac tatcgcttta tgttactctc tactgaagga tgatgatttt180aattctcggc gcagacgaaa atggacggta tctggatcat tgtctttcgt aatttcatgg240gttcttatca tttcaatatt tgtcatcagt gcttatctgt tcgaattttc catttcgaat300aactccatta ctagttctgt aggtttcacc aagaaggagt tcacttggtt cctaaacaca360tctgttaaat acagtattta ttttggagta gcattcttct ttgtagaagt tctacacagc420atagttgttg cactgtatgc acgtcgattt tggcgtaagc tctatgaatt agtgggtgtt480attgtcatca gctttattgg tttagctgta ctgatgagca gtttggtacc atttgcatca540ctgaatccaa ctatacaatt accgtctcaa aatcttcagg tttataaaca tttgcagcct600tattatatca caaatagtta tggtctattc cgccggatga ctggagttgg tggtcgaccg660gaacttatct tggaagctgc atcaaatctg aatggtccgt ggtctgagta cgccttcaac720ttcaaacctg gtcgtgtaga tgggagacct ccggttgtca tcccacatca gcctcgttta780gattggcaaa tgtggtttgc tgcgctcagt aactatcgta atcatccttg gtttatgaat840
ttaatttatc gattactgaa ccaacaacct gaagttttac aactgctcga tccatcgggt900ctgccgaata atccgaagta tattcgagct catctgtata cataccattt caccgattct960gctaacagca aggattggtg gaaacgtaca ttcaaatctg aatacctgcc accagtctca1020ttgtcttctg aaatattgcg tagctctatt caagaaaatg ggttggtagg aaaacgtcat1080cctcgtccac atgatccaac aattttgtca ctatcgttat ctcgtctcag aagttttgtt1140ggacaaccaa aagatttatc tcctttggtt atggtgtgct tagtgttagc aataactaag1200tttgcagtta atcaagctga ccaatcgact agaacaactt accatcacaa tagagcttga1260tgtgtttttc atagttcaaa ttattcaagg aataattcat cgtgtgttga ttatgatgaa1320gtatttgttt gtatttatgt attatttctt tgaatgcgta tagacgttca tattcttccc1380attccaatgt ttgttctact ctcctatatc catttagttt tcatattatg tatactcctg1440acaaaattca ataattcaac cggtcgtatt tgaaataaac tataaacttt tcattcatct1500attcttaaat tcaataaaag cgtaatctgt ataattaaat tattccttat accgtgtggt1560atttgaatct ttttgttaac tctctgttca tatatttatg tgtgtgttga attttcactg1620tgacataaat gtattcttcg gtcatcattt aattcatagt tctggtcatc tgtttcaacc1680tctttttttt tgtttggtcg taatacatct gaatagttat tttcactgtt catcttgtga1740ctctgagtta ttacctaata attgtttcat cgtaaataga cacaaaatta attactgtta1800ctcggattta cttagtatca tgattataaa tatctataga tttattcttt c 1851<210>20<211>1087<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>20gagaatatca gagttcacaa tcacatctcc gtctaaagat atatctcgtt tgttgaactg60tgtattagtg gaactatttt tgaatcactt ggtgttgcca agtcatattt ttactttcaa120gtgcctgaat acattcttac caacaatggg tcgtaaatac ccttcagaga cacctcgtag180tgcaatgcgt ggccaatgtg aaggatctag tgttttggta cagcgtactc ataaatcaac240ttccttagaa agtagtgcga attggtttaa agaagaagtc aggtatcaga tggaacgagg300tggtttgttt gaagatccat tttttcctgc agttgactca agtatcaaat caggatctag360atcgggtagc caaagttaca ggtggcttag acccagtgaa cttacacggt gcccgcgttt420tattgcagat ggagttagtc gcttcgatat taaacaaggc gagcttggaa actgttgggt480tatagctgca ttagcttcat tatcaatgta tccagattta ttcaaacaag ttgtaccacc540tgaccaatcc tttgagaaat cgtcaaaata tccatacgtt ggaatgtttt ggtttcgact600ttggcagttt ggtaattggt atgatgttgt tgtagatgat cgtttgccaa ctcgtaacgg660acatttggta tttatgcatt cagcagattc taatgaattt tggtcagcct tattagagaa720agcctatgcc aaacttgttt cttcatatga tgcacttcga ggtggatgta cagcagaggc780gatggaagat tttactggtg gtcttacaga actcgttgat ttaggcacga aaactcctgc840taatctatat ggtatgatgg aacatgcact taatcgagcc tctttaatgg cttgtagtat900tgatgctgat ccacatgaga ttgaagcaaa tggcccgttg ggattaattc ttggtcatgc960ctattctgtg acagatattc gagaggtgca cactaattat gaatctcgaa gcgttcgttt1020aattcgactg cgtaatccat ggggaaatga ttgtgaatgg tcaggtccat ggagtgatca1080gtctaaa 1087
<210>21<211>1624<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>21taactcatgt gaaattattg cactcaatct attttatatt actttacata acaataaaaa60ttttattcat ttgtactatt tcattagtat tatacacaca ttactactta tttatttatt120taattcattt caattattat tgtaaagtcg aattgattca ttgacagttg aatacattca180gaacttctta atagttacaa cagccacaga agaaaactac acttgaaagt atcatgttca240gttatattcg tctattatgt ctattgttgc atggaataac agcattaaat tatccaatga300atgattatgt accatcaaat atgtggactc ctgattgtat tccattaaat ttaccagtat360tcactagtga agaagcaaaa aatccattga ataataaaga atatgcatta ccaaatgaaa420tgaaagataa aagtttacaa gaatatggat tagatatgga tgatgaatat tctattccag480agttgactaa tttagctgtg atacaagaag aaaacaatta tggttattcg tatcataatg540ataatcagaa ttatcaacaa gttaatgatt ataatgataa taacagtaag ggtaatgata600atgatgaaga tgatgtcgta atacattttg atagtgaaca cggcaataac aataatgata660aaaaacgtag atattattat tataaacaga gaaaacaaac tgataattca atgagatata720tcagtaaatc atataagaaa tctaagcttc tgtatggaac aggttttatg ccaacacttt780ctgtaccagt ttcaataccg gtacaagcac aagttccagt ttcagtacca gtagcacaac840catacagtat aggtggacat gcttacatgc ctggtcattt tcattatggt tttcctgttc900aacctatgca accagtaatg ccacctcagc aaccacctcc aatgatgcca cctcaagttc960ctacaatggt accaccacaa caacctccaa tggtgccacc tcaacaacct ccagtgatac1020cacctcaaca accacctgct gtaaccccac agcaaccacc tgctgaaaca ccacaacaac1080caccaacttc agtaacacca cagcaaccac ctgctgtaac tccacaacag ccacctggaa1140taccaacaca aactcaacct gtagcaccaa cactaccatt atcaccaact caaccagttt1200tacaaagtca aacacaaact caattaagta tccaaccaca aataccacag gtaccacaaa1260ttcctcaatt accattgact ccacagcatc aattaataca attacaacaa ccaaaagcac1320ttacagttaa acatgttata ttgcctcaac aaatctattt aacagttaaa agtacaagag1380ttaaacccag gttcattaaa cgaatatatt attaatgttg tatagatgac aataatagta1440gatgataata ataataataa taataatatg aatattaggt aaactttaat aatttacttt1500cactttattt tggtgtttat taaagtcggc attaacaatt aattgacaaa tcaatcagaa1560tcgtgaaagt tactttaaga tgtttgaata acagaaggaa aacattaaga gagaggaaaa1620aaaa 1624<210>22<211>913<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>22ataaaataga cattaactta aaagcactac tataatctta ttatagttga agtgtaaaac60aatgaaaagt gttgcaagtt tgagtttatt catcatttta ccatgtgtat tggcagctga120ttataattca tactatcaaa atgataatta cggtggaaat tatgataatg gagattacaa180
taataataac aataatgatt attacaatgg tggaggtgat aattatggaa atgataataa240tggcaaaata caaacaattg gaaaatttgt tgccaacgga aatgctgata aaggcttatc300atataatcaa gttacatatt ttcataaagg tagcaaatat aagaagaaat ttgcaaatta360tgatacaaag ggtaaagtac atagttatgg taaacagaat atcaaagcga aattccatgt420cgaaagtaaa ctgcatagta aagggaagta taatggtggg aaattcgata atcttgtcgg480cggtggtggt gatattggtg gtaataacta tgcagaagga caaataccaa tagacaatgc540tgcatatggc agtggtccat caaatacccc gtcaaataat gataattaca ataacaatga600caattacaat aataatgaca actacaataa taaggacaat tataataaca atgacaatta660tggtgaaggt tcaggaggag gcggtgaata ttcaggtgat aatggatatt ccaaaggatc720atcttacgat gataacaatt ataataataa taataacaac tattattaat gactatagta780aaggacaagg atataagtca agttcatttt tatttgttta ttaataaaga tgaagtacta840tgtatggcaa gaagtaaact gaataatcac taattgttta cagaaaaaaa aaaaaaaaaa900aaaaaaaaaa aaa 913<210>23<211>2267<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>23ccgagcacca ccttgtaact gttgaatata cacttaacat atacacaaac acatttcttc60aagcattgaa tcatgtctct tcaaagaaaa cgcttcctcg aaggtagtat tagcaatgat120aataatcgca gtggcagcag caatgatgtt aatcaattcc aaagagactt ggagtctgaa180aagcatctat tgaggaactt agtaatcata ttatttggga cattactttc tgttatattg240ctatcactac ttatatatgt tatatgtcgt cgatttcgaa agcattcaaa agtacttaat300ggcaaaagta aacaagatga tgacgatgaa tacatttgtt ttactaaatc agataaagct360ccatacggtc gatatacaca actacaaaca cctgaaacta cattgacttc agtaaacatg420caattacaga cagtgaatag atcaaatgat ctatttaacc atgacaaaat gatctattca480gcagaagatg agatttatga tgatgaatta aatgaatata aaccgactta cggttactca540acacgtcatc agacaagtta cattaaatca aatgtcatgc agtcgactca acagtatatt600gagtcggctg aactaaaaag aagaaatgaa aatttatgca aattgaccag tgatcaggaa660aagccagaat catgtttgct aatagagaaa aatcgtcgaa gtattcgccg tgtgagtagt720acacctaact taacgaaatt tgttaacaat cagtcaaata atcttgatac caatcattta780cttgatcaca atgaagaagg ggatgaagac aaagatgaca aaaatggctg tgcacttgat840gattataaat atttcaaagt gaatgaggga aacgatgagg atattgctga cttaacattg900attgataaca tggaggaacc aaacaagaac gaaggatcag aatcattgga aaattcaacc960aatccactag cattgatacc aattattctt ccaaattggg caattgaacg tggagtacaa1020gaaagaggct atattacgct agctgtacaa gttgaaggtg atttaagtaa catcaaaaag1080tatccatttc tgtctgtacg catatttgat gtacgttgtg tgttgagtaa aaatgtagaa1140ccaaaagctg gaaaattcta tgtgaaagca cgtcttcaat tagctggtac caacaattcc1200gcctcattgt tatcattgga ccaaatctca acaaagtcat taaaagttct ttctgataaa1260atagatatgt ttacaaccaa tagaaattct actacagtga ctagtggtcg gacacctgtc1320agacgtgcct atcgttcacc agtgtttcga cataaaattc tcctatccct tccatcagat1380gtaatagata taatcaatag tcgaccaact tcatttgatg agtctacaga gtcattttca1440
tcgacgaact tagaaaaaga tacagtgata caagattatg aactgaaaat cgatttgaaa1500gaacgtaatg cacgtaaagt aaatgatctg catggaacta gtactgtcca atatttacca1560tactggaaat cttctcagtt gattggtagt gtgcgtattc ctataacaac taaaatgtgg1620caatatctgt tagaagatgt gaataagcta cgtcgtggca cagttgtgaa tgcgtctatc1680tgtcaagtta gcaatacatc gaataaattg ttatggtttg tgagatgtct acaagtttca1740gatgagaatg tcggttcacg gggagagatt actttcggta tgcaatataa tcaagaaaat1800ggtagtttaa ccattagcct attcaattgt gctgcaatga atttaccaaa acgaacaaaa1860aatttatttg ttcgtgcttc attatgttca aattggaaat taattcgaac tgttcaatcg1920agaccaacag ctagactgtc agtgaattca gctgaattta tattaggtga aaaattgagt1980tttattattg aagacgatgt tagtcgtgtc tgtataatat taaatgtatt tgcacgatat2040ggaagtaaac tacaagaggc tactacattg attggtcgtt gtgtaactgg accatcagat2100ttagcatgcg gtgatggact ttcacattgg acatcagtat gttctaaacg aggcatgata2160agacgtacac atattctgtt gtagatgaat ataacattta tttgagtggt aactttgcat2220tccatactga aatgaatgtt ttgaagttta aaaaaaaaaa aaaaaaa 2267<210>24<211>484<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>24FNRHISDLDF YVNYVDGYST SFQGPNYNLS HITTKNVSMA VKRYPLAEPR GIKAHFIMDH60NGILVLSGVS CLFHPIEKNE VSDNSTKEKS AFEKIGSTLS GLFGGGSSSS GNDKISPEQD120TPINTTNNSD VNPTGANSVN FTNNSAFSEN QTRSHVDDNV SGEDQATVVV KSTVPFSEPI180DSEVVYVDFP DLTSKQFTLS QSKLHELREA DRAKAALEQS INQLETLLID THDKILTDYK240MYGTESELSQ LDNTLQSASS WFYEQGHDGL KSDYDLRIRE IQSLLNPIER RFNEVKYREK300AILSFDKTIG LAQKTLNEMI ELSQLFSRTL ETISPELQQQ KPSTSTSEQA TKESPWKNIF360NEAEIDILRN KINESKIWLA ESSEALNKSL TYQDPPVLVS AIQSKEAELS RETVYHTTKI420NLWRSEIARM FAEQSKINAT SVPLKEEAQT PPSSSDEETI PKPSTINEDQ EPTLKPTQTP480RPEL 484<210>25<211>213<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>25MAVLRVLCGL LLVSILFLGF VLSEANGPKV TEKVFFDIEV DKQPLGRIII GLFGKTVPKT60VENFKQLSIG TTLKDGRTAA YKGSTFHRVI KSFMIQGGDF TNHDGTGGFS IYGERFPDEN120FKLKHVGAGW LSMANAGPNT NGAQFFITTT KTPWLDGKHV VFGKVVEGMS VVRQIENMQT180DSRDRPVKSV KIANCGHIPV DVPFSVSNTD AVE 213<210>26<211>212
<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>26MALNQLSFLV DKAKKEYPSS LKFLMCCSAA FVSETITYPL DMLKTRTQVY GELQSSSYPG60VVRICSSIIK NEGLFQLWQG LSPALIRHII YTGARMPIYE IIRQDIFHLP PASHFTVKSS120KNFYSETNSS ITLHNKLPAT KTTDYELMQE NKSDFIIRAA LTGMIAGSFA QFLASPIDLI180KVRLQTERKW MSEIHPNNYK PSKPTEVPVR YQ 212<210>27<211>226<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>27MKINIDGLLV YFPYEYIYPE QYHYMIELKR TLDAKGHGVL EMPSGTGKTV SLLSLIVAYM60KARPGIVEKF VYCSRTVPEL EKVIDELKVL DKYYADETNE KGCGLLGIIL SSRKNLCIHR120DVKHAGDGAA VDSACFRLTA SFVRKNRIAD PNVAYCKYYE EFDLNGRDNP FAPGIYSMAD180IKAYAKNITI ALTFLQKCYF LCKYYRLQLL LPTRSKIANV ALGITQ 226<210>28<211>433<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>28MMWLSLLFML VAPFVAANED KRKNLLVLLD DVYVKETHST FLKMLTSRGY NLTTKLADDS60SLSLKHLGEY LYSGLIILAP SVSEFGGTIN VNEITNFVDD GGNLVVVAGP NVGESIRELG120SDCGVEFDEE NTFVIDHFNF DKKDSGLHSL ITVPVSNLIK SKVITGGKVN APILYRGIGI180SADQTNPLLI DYLHGSKTSY SYNPDKSVTD YPNTVGTNTQ LITALQARNN ARALFIGSLD240FLSNSFFEST VETESKMSVL CGNQVLTENL MRWVLGECGQ LRYTSVKHHR VDETVPPSQY300TIMDDVVYTI KIETKDDSGN WIPYDADDVQ LEFVRIDPFI RKNMEHKDGE YKLVMKLPDV360YGVFKFVVDY YRVGYTHLLS VVQVPVRPFT HTQYERFLVA AYPYYTSALS MMVGLVLFSF420VFLYLKDDKE KGE 433<210>29<211>128<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>29MPRRGASPSR APSRPYASLP ARTPQSAVAH PPAQPRQPGL MGQMAATAGG VAVGHVVGSA60ITGAFSGGAG SSPAQSSAPA VPSDQYQSPC QFQVDELIKC AQTQSDISAC SGFSEALKEC120SRHYGLYQ 128
<210>30<211>265<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>30MKHNGPTPPA SSSLLSLLFG YLLCNSLLVG GNANRYYSSQ AVPYKQQTNQ YYGYNNEGGS60DDTYGNDNYD KNTNKYSTNN NDNYNNYGDN NNYGSNDYNA KSYDNYNGNN NNNDYNSDYT120PTKDNYEANK YKEEYTPSNE FVPPGGSIDD SSMPSFTTGT ISGSKHSHGR LDVRGKMAGV180GHQDYGVNYL SLTKFRKGGG YDIYGKKRQF LDYDTQGHVK KYGNQHMRTK FEVVGNLNGY240STYKQKSSFT PPSGGYSSKR GRRSY 265<210>31<211>423<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>31MRRIIIFLYF VQFFGQWRYK LCISIYIITS IFFIIAYLSG SPSRIVYSHP DELIDDALCS60LPEPEVCCPN KTDLDLSQFS SISPTDILNI LNVTQHVCDG SWKPKCLFSQ KIAIIIPYRD120REKHLKLLLP RLHALMLRQN MPYYVFVIEQ AGTTPFNRGL LLNVGVLYAL EIDPEVNCFV180FHDVDLLPEK SENLYLCDTE LRHLSPAIDE FRYHPPFINY AGGVAAMSKE NIFKINGFPT240RHWGWGSEDD EFSARGLIFN LKLTRPPEHI GRYKAPRHRK GSISFGHQSA FLKFQNYLHD300GLTLLRDNFY PKWKVHSLNN RINKFSLNNL EKFKIPAFVN QCTRENILSQ LNLDKNSTEI360VHDLVKISSY NMKLLNHFSR DNLFKHFIFD PTDLYNQSEF ILKPQGNHTD ETVWWFLHFY420GWI 423<210>32<211>466<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>32MTLFNIKQLI VIIYIISLSQ CLGYIVLMSS EGYRYGHQLQ NPLPYYTNEE DIISSNDDAS60IYDGESGEYI DYHVSPYENV IDDTTIPYQN DEYEEGEAYS QDYPNVDYQT YNDENSQYYG120PVEYNNMQYD QSSYDYNMPY NAYYSRRDTN DYYQERPDIQ YRVSLGNRPY QYQEDYDDDD180AADYYSNEGN YEDDYNSNND NYKSQSDSNV NFRQYYKLPT ASYKHQKYVP HRLDTLKDYQ240PIDDKLYVFY PKDIKPQYYT LRKMHTKYGK LNPYFSSRII SKKSPNGYFK NVLPTTYQSD300DHHKHRDNSM HSNKNNHYYY KSINKSPNYK SIDNYSPTSN YKDHHLNGRS SSYYRNNNKN360EYRPSKSKVS YPTTNNKKNK PLRDVYGNSM RTTDYEETIS YHKSGEKGPK GKMKSYSSYD420IYGHLKHTGK SKLGSGITRY HTVIDSDKSK NRHYVTYQPL NNEYNK 466<210>33
<211>166<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>33MIRLSTSVLF IILGIIYSLK ANENYPSKQT ADGSWCDDNT EHDYCSPYFV ICTTKQYTRR60CLSKYTFGGE GPEYENKENI TFTEQLGENI TNPLQFTMPE WSNDTVIHVA VFNKGLNVVS120LLGRSDILID WIETPGTNES EKWQEVYFTA DESEMALDAY VKVFTS 166<210>34<211>73<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>34MMVPSIFYME LSLYYGSGVG WTFYPPLSSL ATSGVGVDYL MFSLHLAGVS SLDWFYKFYY60YYNVASKVMF FSY 73<210>35<211>181<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>35MGGLLTTIKS YFSKMEARIL MVGLDAAGKT TILYKLKLGE VVTTIPTIGF NVETVEYKKI60SFTVWDVGGQ DKIRPLWRHY FQNTQGLIFV VDSNDSERIG EAADELHRML GEDELRNAVL120LVFANKQDLP QALSPADITD KLRLRSLHNR DWYIQATCAT SGSGLEEGFE WLSHTISQKK180Y181<210>36<211>153<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>36MRLLVRSHLG GSIVVSAGPN ESVKSLKDKI SVATGIHAST QSLYHHGLPL LDSSLLEELD60FTDLDNVDVN CELLGGGKKR KKKNYTTPRK IKHKKKKVKL ATLKFYKVDS NGKITRLRRE120CPSEKCGAGV FMASHFDREY CGKCALTYKH VNK 153<210>37<211>343<212>PRT<213>日本血吸虫(Schistosoma japonicum)
<400>37GIPGTGKTAS VQAVLSTMHK LVADSCLESQ LPVFQTIYVN GMRVSDPKQI YIQIYEQLTG60LIATTKSACD LLEKEFCSST NKKLNHREVS EKPVILVIDE LDLLCTRRQD ILYSLFDGPT120RHNNRRVLIV LAIANTMDLP ERLLHPRVAS RLGLTRLTFA PYSHEQLSQI VRHRLSSLSN180ILQPKALELA ARKVAAVSGD VRRALDICKR AAEIVSSSEK TNKEIDISHI NAALKEMFVT240PKSDAICACS LYEKLFLRAV IAEFQARSTE EARLDRCIRQ MSALCRLEGV PCPTTSEVFA300ICASLGAHKL LLTERSRYDI AMLVRLNCTK SDILYCLNSQ SNG 343<210>38<211>138<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>38MVSLKVDNLA YRTTIDDLRR VFSRFGEVGD IYIPRDPYTF ESRGFAFVRY CTDREADCAI60RGMDGHKVDG REVRVQRAKY GRPTPNRRRR SVTPRRNGRR SRSFSSGRDR RSPRRINDRY120RLPSSPRHYS RSLSRERR 138<210>39<211>193<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>39IFAFITFICS NKADKFPTKN ILTESYNDDY KGLYNEKHHD RKDPQDYWKP NHEDKKNYES60NGYGENGNKY VDEGHGKDSY PGYPSSPHET YKPNKRDDKR KYDVHSYDDH YDYFKDFDKH120LYGDNHNNQP SHDKYPEKTY QNSDEPHTDD YDGNQIKPEY NNYQNEYNEH YQPQQNDYYT180NSGYGSEGDY YYV193<210>40<211>302<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>40VVHTVDLHSS CCTMRPFVIG FLFSLILIIR SSQIDQSNTV FIGSITARDK DHFRAVFAKH60LSELPSEAFI IYHAILGSHC LDVKVPNIDS ICSTLNKPVT NAEESFYASS IYKLTGSSKC120KVSTAEVETL SKQLLVEDIP IESLFYLISS MKNLDIKIDA NRVSTILGKI KAKDTSPMTL180SFMLHILSQL GLTKSALSGY SSSVGDVLDQ AGEISDNQLF YIKGLYTSAF VAKSVVEFLT240AYGEVPKGVE NKLVKLFNYL YTRRQTTNIR ASAYLVAAFK SLAYSSLLMP VVIESSVKTN300ED 302<210>41<211>265
<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>41MISLSKKVAI VTGASSGIGR ATAILFAKLG ACVALVARDK SRLQETRQAC IQVSHPDVYE60KHKEPFLCIE ADLGDSHEVE KAYRLALNHF HQLDILVNNA GFMIQDTVET FNIDEYERLM120KLNLTAAITL TNLAVPDLTT TKGSIVNVSS VCGERSFPGV MSYCISKAAL DQFTKCTALD180LASKGIRVNS VNPAVIVTEL HKRAGMSEDD YKKFLLKSKE SHALGRTGTV EEVAQAIAFL240SSSASSFTTG NLLMVDGGRS IMCPR 265<210>42<211>419<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>42TFVIEIPMTF LFFMPFRTTR LASFYSQVLL QLAIILTGNY NFFNLLTIAL CYSLLKDDDF60NSRRRRKWTV SGSLSFVISW VLIISIFVIS AYLFEFSISN NSITSSVGFT KKEFTWFLNT120SVKYSIYFGV AFFFVEVLHS IVVALYARRF WRKLYELVGV IVISFIGLAV LMSSLVPFAS180LNPTIQLPSQ NLQVYKHLQP YYITNSYGLF RRMTGVGGRP ELILEAASNL NGPWSEYAFN240FKPGRVDGRP PVVIPHQPRL DWQMWFAALS NYRNHPWFMN LIYRLLNQQP EVLQLLDPSG300LPNNPKYIRA HLYTYHFTDS ANSKDWWKRT FKSEYLPPVS LSSEILRSSI QENGLVGKRH360PRPHDPTILS LSLSRLRSFV GQPKDLSPLV MVCLVLAITK FAVNQADQST RTTYHHNRA 419<210>43<211>314<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>43MGRKYPSETP RSAMRGQCEG SSVLVQRTHK STSLESSANW FKEEVRYQME RGGLFEDPFF60PAVDSSIKSG SRSGSQSYRW LRPSELTRCP RFIADGVSRF DIKQGELGNC WVIAALASLS120MYPDLFKQVV PPDQSFEKSS KYPYVGMFWF RLWQFGNWYD VVVDDRLPTR NGHLVFMHSA180DSNEFWSALL EKAYAKLVSS YDALRGGCTA EAMEDFTGGL TELVDLGTKT PANLYGMMEH240ALNRASLMAC SIDADPHEIE ANGPLGLILG HAYSVTDIRE VHTNYESRSV RLIRLRNPWG300NDCEWSGPWS DQSK 314<210>44<211>393<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>44MFSYIRLLCL LLHGITALNY PMNDYVPSNM WTPDCIPLNL PVFTSEEAKN PLNNKEYALP60
NEMKDKSLQE YGLDMDDEYS IPELTNLAVI QEENNYGYSY HNDNQNYQQV NDYNDNNSKG120NDNDEDDVVI HFDSEHGNNN NDKKRRYYYY KQRKQTDNSM RYISKSYKKS KLLYGTGFMP180TLSVPVSIPV QAQVPVSVPV AQPYSIGGHA YMPGHFHYGF PVQPMQPVMP PQQPPPMMPP240QVPTMVPPQQ PPMVPPQQPP VIPPQQPPAV TPQQPPAETP QQPPTSVTPQ QPPAVTPQQP300PGIPTQTQPV APTLPLSPTQ PVLQSQTQTQ LSIQPQIPQV PQIPQLPLTP QHQLIQLQQP360KALTVKHVIL PQQIYLTVKS TRVKPRFIKR IYY 393<210>45<211>235<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>45MKSVASLSLF IILPCVLAAD YNSYYQNDNY GGNYDNGDYN NNNNNDYYNG GGDNYGNDNN60GKIQTIGKFV ANGNADKGLS YNQVTYFHKG SKYKKKFANY DTKGKVHSYG KQNIKAKFHV120ESKLHSKGKY NGGKFDNLVG GGGDIGGNNY AEGQIPIDNA AYGSGPSNTP SNNDNYNNND180NYNNNDNYNN KDNYNNNDNY GEGSGGGGEY SGDNGYSKGS SYDDNNYNNN NNNYY 235<210>46<211>703<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>46MSLQRKRFLE GSISNDNNRS GSSNDVNQFQ RDLESEKHLL RNLVIILFGT LLSVILLSLL60IYVICRRFRK HSKVLNGKSK QDDDDEYICF TKSDKAPYGR YTQLQTPETT LTSVNMQLQT120VNRSNDLFNH DKMIYSAEDE IYDDELNEYK PTYGYSTRHQ TSYIKSNVMQ STQQYIESAE180LKRRNENLCK LTSDQEKPES CLLIEKNRRS IRRVSSTPNL TKFVNNQSNN LDTNHLLDHN240EEGDEDKDDK NGCALDDYKY FKVNEGNDED IADLTLIDNM EEPNKNEGSE SLENSTNPLA300LIPIILPNWA IERGVQERGY ITLAVQVEGD LSNIKKYPFL SVRIFDVRCV LSKNVEPKAG360KFYVKARLQL AGTNNSASLL SLDQISTKSL KVLSDKIDMF TTNRNSTTVT SGRTPVRRAY420RSPVFRHKIL LSLPSDVIDI INSRPTSFDE STESFSSTNL EKDTVIQDYE LKIDLKERNA480RKVNDLHGTS TVQYLPYWKS SQLIGSVRIP ITTKMWQYLL EDVNKLRRGT VVNASICQVS540NTSNKLLWFV RCLQVSDENV GSRGEITFGM QYNQENGSLT ISLFNCAAMN LPKRTKNLFV600RASLCSNWKL IRTVQSRPTA RLSVNSAEFI LGEKLSFIIE DDVSRVCIIL NVFARYGSKL660QEATTLIGRC VTGPSDLACG DGLSHWTSVC SKRGMIRRTH ILL 70权利要求
1.一类日本血吸虫雌性成虫特异蛋白，其特征在于，选自下组(a)具有SEQ ID No.24～SEQ ID No.46所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
2.如权利要求1所述的蛋白，其特征在于，它具有SEQ ID No.24～SEQ ID No.46所示的氨基酸序列。
3.一类日本血吸虫雌性成虫特异蛋白基因，其特征在于，它编码权利要求1所述的蛋白。
4.如权利要求3所述的基因，其特征在于，选自下组(a)SEQ ID No.1～SEQID No.23所示的核苷酸序列；(b)SEQ ID No.1～SEQ ID No.23所示的核苷酸序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.23所示的核苷酸序列中每条序列有至少70％同源性、且编码相同功能蛋白质的核苷酸序列。
5.如权利要求3或4所述的基因，其特征在于，它具有SEQ ID No.1～SEQ ID No.23所示的核苷酸序列。
6.一类日本血吸虫病防治疫苗，其特征在于，所述疫苗以权利要求1所述的蛋白为免疫原。
7.一类能与权利要求1所述的蛋白特异性结合的抗体。
8.权利要求1所述的蛋白在制备预防和治疗日本血吸虫病的药物中的应用。
全文摘要
本发明公开了日本血吸虫(Schistosoma japonicum)雌性成虫特异蛋白及其基因序列，还公开了与该蛋白特异结合的抗体，以及把雌性成虫特异蛋白作为免疫原制备所得的日本血吸虫病防治疫苗，此外，本发明还公开了日本血吸虫雌性成虫特异蛋白在制备预防和治疗日本血吸虫病的药物中的应用。运用本发明的蛋白，可抑制血吸虫雌性成虫的产卵，从而治疗血吸虫病、防止血吸虫的扩散。
文档编号C12N15/12GK1986564SQ20051011172
公开日2007年6月27日 申请日期2005年12月20日 优先权日2005年12月20日
发明者韩泽广, 刘锋, 王志勤, 胡薇, 冯正 申请人:上海人类基因组研究中心, 中国疾病预防控制中心寄生虫病预防控制所